title: Lunasin alleviates allergic airway inflammation while increases antigen-specific Tregs.
abstract: Lunasin is a naturally occurring peptide isolated from soybeans and has been explored in cancer treatment. Lunasin inhibits NF-κB activation and thus pro-inflammatory cytokine and mediator production in macrophages. In this study we demonstrate that lunasin can effectively suppress allergic airway inflammation in two murine models of asthma. In an OVA+Alum sensitization model, intranasal lunasin treatment at the time of OVA challenges significantly reduced total cells counts in bronchoalveolar lavage (BAL) fluid and eosinophilia, peribronchiolar inflammatory infiltration, goblet cell metaplasia and airway IL-4 production. In an OVA+LPS intranasal sensitization model, lunasin treatment either at the time of sensitization or challenge has similar effects in suppress allergic airway inflammation including significantly reduced total cell and eosinophil counts in BAL fluid, inflammatory gene Fizz1 expression in the lung, and IL-4 production by OVA re-stimulated cells from mediastinal lymph nodes. We further show that intranasal instillation of OVA+lunasin significantly increases OVA-specific regulatory T cell (Treg) accumulation in the lung comparing to OVA only treatment. Taken together, our results suggest lunasin as an anti-inflammatory agent can be potentially used in asthma therapy or as an adjuvant to enhance the induction of antigen-specific Tregs and thus boost the efficacy of allergy immunotherapy.
authors: Department of Pediatrics, HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America; Department of Veterinary Medicine, Southwest University at Rongchang, Rongchang, China.
authors: Xiaowei
authors: X
authors: Yang
authors: Department of Pediatrics, HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America.
authors: Jingjing
authors: J
authors: Zhu
authors: Department of Biology, School of Science, Indiana University Purdue University at Indianapolis, Indianapolis, IN, 46202, United States of America.
authors: Chun-Yu
authors: CY
authors: Tung
authors: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America.
authors: Gail
authors: G
authors: Gardiner
authors: Department of Pediatrics, HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America.
authors: Qun
authors: Q
authors: Wang
authors: Department of Biology, School of Science, Indiana University Purdue University at Indianapolis, Indianapolis, IN, 46202, United States of America.
authors: Hua-Chen
authors: HC
authors: Chang
authors: Department of Pediatrics, HB Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America; Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, 46202, United States of America.
authors: Baohua
authors: B
authors: Zhou
mesh_terms: D000534:Alum Compounds; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000150:complications / Q000188:drug therapy / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology; D005260:Female; D020397:Goblet Cells / Q000187:drug effects / Q000276:immunology; D006967:Hypersensitivity / Q000150:complications*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D030262:Soybean Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology*
chemical_list: D000534:Alum Compounds; D000941:Antigens; C120514:GM2S-1 protein, soybean; D008070:Lipopolysaccharides; D030262:Soybean Proteins; C446402:ovalbumin-alum; D010047:Ovalbumin
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