title: Growth hormone-releasing peptide-biotin conjugate stimulates myocytes differentiation through insulin-like growth factor-1 and collagen type I.
abstract: Based on the potential beneficial effects of growth hormone releasing peptide (GHRP)-6 on muscle functions, a newly synthesized GHRP-6-biotin conjugate was tested on cultured myoblast cells. Increased expression of myogenic marker proteins was observed in GHRP-6-biotin conjugate-treated cells. Additionally, increased expression levels of insulin-like growth factor-1 and collagen type I were observed. Furthermore, GHRP-6-biotin conjugate-treated cells showed increased metabolic activity, as indicated by increased concentrations of energy metabolites, such as ATP and lactate, and increased enzymatic activity of lactate dehydrogenase and creatine kinase. Finally, binding protein analysis suggested few candidate proteins, including desmin, actin, and zinc finger protein 691 as potential targets for GHRP6-biotin conjugate action. These results suggest that the newly synthesized GHRP-6-biotin conjugate has myogenic stimulating activity through, at least in part, by stimulating collagen type I synthesis and several key proteins. Practical applications of the GHRP-6-biotin conjugate could include improving muscle condition.
authors: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea; Peptide R&D Center, Incospharm Corporation, Daejeon 34141, Korea.
authors: Chae Jin
authors: CJ
authors: Lim
authors: CRID Center, NeoPharm Co., Ltd., Daejeon 34037, Korea.
authors: Jung Eun
authors: JE
authors: Jeon
authors: CRID Center, NeoPharm Co., Ltd., Daejeon 34037, Korea.
authors: Se Kyoo
authors: SK
authors: Jeong
authors: Peptide R&D Center, Incospharm Corporation, Daejeon 28674, Korea.
authors: Seok Jeong
authors: SJ
authors: Yoon
authors: Peptide R&D Center, Incospharm Corporation, Daejeon 28674, Korea.
authors: Seon Deok
authors: SD
authors: Kwon
authors: Peptide R&D Center, Incospharm Corporation, Daejeon 28674, Korea.
authors: Jina
authors: J
authors: Lim
authors: Peptide R&D Center, Incospharm Corporation, Daejeon 28674, Korea.
authors: Keedon
authors: K
authors: Park
authors: Departments of Pharmaceutical Science and Engineering, Seowon University, Cheongju 28674, Korea.
authors: Dae Yong
authors: DY
authors: Kim
authors: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors: Jeong Keun
authors: JK
authors: Ahn
authors: Departments of Cosmetic Science & Technology, Seowon University, Cheongju 28674, Korea.
authors: Bong-Woo
authors: BW
authors: Kim
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D024510:Muscle Development / Q000187:drug effects; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D009842:Oligopeptides; C041048:growth hormone releasing hexapeptide; D007334:Insulin-Like Growth Factor I; D001710:Biotin; D003094:Collagen
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