title: Proteomics-driven analysis of ovine whey colostrum.
abstract: The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.
authors: Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Domenica
authors: D
authors: Scumaci
authors: Dpt. of Health Science, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Francesca
authors: F
authors: Trimboli
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: Ludovica
authors: L
authors: Dell'Aquila
authors: Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Antonio
authors: A
authors: Concolino
authors: Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Giusi
authors: G
authors: Pappaianni
authors: Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Laura
authors: L
authors: Tammè
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: Giorgio
authors: G
authors: Vignola
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: Alessia
authors: A
authors: Luciani
authors: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM) "G. Caporale", Teramo, Italy.
authors: Daniela
authors: D
authors: Morelli
authors: Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Giovanni
authors: G
authors: Cuda
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: Andrea
authors: A
authors: Boari
authors: Dpt. of Health Science, Magna Græcia University of Catanzaro, Catanzaro, Italy.
authors: Domenico
authors: D
authors: Britti
mesh_terms: D000818:Animals; D003126:Colostrum / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D063990:Gene Ontology; D007774:Lactation; D008894:Milk Proteins / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D040901:Proteomics; D012756:Sheep / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins
references: Brain Res. 2000 Sep 29;878(1-2):174-82
references: 10996148
references: Int J Gynecol Cancer. 2014 May;24(4):649-58
references: 24614826
references: Br J Nutr. 2000 Nov;84 Suppl 1:S75-80
references: 11242450
references: Traffic. 2000 Oct;1(10):769-82
references: 11208067
references: J Nutr. 2001 May;131(5):1433-7
references: 11340095
references: Eur J Biochem. 2002 Feb;269(4):1209-18
references: 11856354
references: J Appl Physiol (1985). 2002 May;92(5):2177-86
references: 11960972
references: Electrophoresis. 2002 Apr;23(7-8):1153-60
references: 11981865
references: Am J Physiol Lung Cell Mol Physiol. 2002 Aug;283(2):L452-9
references: 12114208
references: J Physiol. 2002 Dec 1;545(Pt 2):567-79
references: 12456835
references: Electrophoresis. 2004 May;25(9):1327-33
references: 15174055
references: Proteomics. 2004 Jul;4(7):2094-100
references: 15221770
references: Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10084-9
references: 15226505
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: J Anim Sci. 1977 Nov;45(5):1146-51
references: 563861
references: J Am Vet Med Assoc. 1977 Dec 15;171(12):1255-9
references: 604324
references: N Z Vet J. 1977 Dec;25(12):361-5
references: 353601
references: Vet Rec. 1978 Nov 25;103(22):489-92
references: 107648
references: Annu Rev Genet. 1988;22:631-77
references: 2853609
references: Biol Neonate. 1990;57(1):30-6
references: 2302435
references: Cell. 1990 Oct 19;63(2):245-7
references: 2208284
references: Food Chem Toxicol. 1996 Jan;34(1):131-45
references: 8603791
references: Endocrinology. 1996 Dec;137(12):5685-93
references: 8940400
references: Chin J Dig Dis. 2004;5(2):45-50
references: 15612656
references: Mol Nutr Food Res. 2004 Oct;48(5):363-9
references: 15672476
references: J Am Vet Med Assoc. 2005 Mar 15;226(6):951-5
references: 15786999
references: J Dairy Sci. 2006 Apr;89(4):1235-43
references: 16537956
references: Proteomics. 2006 Apr;6(7):2208-16
references: 16502470
references: Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10385-90
references: 16801554
references: Curr Pharm Des. 2007;13(8):813-28
references: 17430183
references: Curr Pharm Des. 2007;13(8):829-43
references: 17430184
references: Matrix Biol. 2007 Sep;26(7):508-23
references: 17560775
references: J Am Vet Med Assoc. 2007 Dec 15;231(12):1873-7
references: 18081529
references: Proteomics. 2008 Sep;8(18):3833-47
references: 18780401
references: Curr Opin Struct Biol. 2008 Dec;18(6):714-25
references: 18948193
references: J Anim Sci. 2009 Apr;87(13 Suppl):3-9
references: 18952725
references: Int J Nanomedicine. 2010;5:1005-15
references: 21187942
references: J Proteome Res. 2011 Apr 1;10(4):1746-54
references: 21361340
references: Vet Microbiol. 2011 Jun 2;150(3-4):373-8
references: 21511407
references: Infect Immun. 2011 Sep;79(9):3833-45
references: 21690237
references: Anim Sci J. 2011 Oct;82(5):698-706
references: 21951907
references: Anim Sci J. 2012 Feb;83(2):162-8
references: 22339698
references: Proteomics. 2012 Sep;12(18):2866-78
references: 22848049
references: J Comp Pathol. 2012 Nov;147(4):441-51
references: 22721818
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D377-86
references: 23193289
references: Biochim Biophys Acta. 2013 Jun;1831(6):1067-78
references: 23388354
references: J Proteomics. 2013 May 27;83:144-59
references: 23563085
references: Mol Endocrinol. 2013 Jul;27(7):1065-77
references: 23660595
references: Curr Protein Pept Sci. 2014 Feb;15(1):64-74
references: 24555887
references: Anim Sci J. 2014 Apr;85(4):440-8
references: 24450292
references: Rapid Commun Mass Spectrom. 2001;15(4):258-64
references: 11223956