title: Antitumor and antimicrobial potential of bromoditerpenes isolated from the red alga, Sphaerococcus coronopifolius.
abstract: Cancer and infectious diseases continue to be a major public health problem, and new drugs are necessary. As marine organisms are well known to provide a wide range of original compounds, the aim of this study was to investigate the bioactivity of the main constituents of the cosmopolitan red alga, Sphaerococcus coronopifolius. The structure of several bromoditerpenes was determined by extensive spectroscopic analysis and comparison with literature data. Five molecules were isolated and characterized which include a new brominated diterpene belonging to the rare dactylomelane family and named sphaerodactylomelol (1), along with four already known sphaerane bromoditerpenes (2-5). Antitumor activity was assessed by cytotoxicity and anti-proliferative assays on an in vitro model of human hepatocellular carcinoma (HepG-2 cells). Antimicrobial activity was evaluated against four pathogenic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Compound 4 exhibited the highest antimicrobial activity against S. aureus (IC50 6.35 µM) and compound 5 the highest anti-proliferative activity on HepG-2 cells (IC50 42.9 µM). The new diterpene, sphaerodactylomelol (1), induced inhibition of cell proliferation (IC50 280 µM) and cytotoxicity (IC50 720 µM) on HepG-2 cells and showed antimicrobial activity against S. aureus (IC50 96.3 µM).
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. daniel.rodrigues@imbe.fr.
authors: Daniel
authors: D
authors: Rodrigues
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. celso.alves@ipleiria.pt.
authors: Celso
authors: C
authors: Alves
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. andre.horta@ipleiria.pt.
authors: André
authors: A
authors: Horta
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. susete.pinteus@ipleiria.pt.
authors: Susete
authors: S
authors: Pinteus
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. joana.m.silva@ipleiria.pt.
authors: Joana
authors: J
authors: Silva
authors: Nice Institute of Chemistry-PCRE, UMR 7272 CNRS, University de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice, France. culioli@univ-tln.fr.
authors: Gérald
authors: G
authors: Culioli
authors: Nice Institute of Chemistry-PCRE, UMR 7272 CNRS, University de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice, France. olivier.thomas@unice.fr.
authors: Olivier P
authors: OP
authors: Thomas
authors: Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. rpedrosa@ipleiria.pt.
authors: Rui
authors: R
authors: Pedrosa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D004224:Diterpenes; D006842:Hydrocarbons, Brominated
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