title: Assessment of MLL methyltransferase gene expression in larynx carcinoma.
abstract: Larynx cancer is the second most common type of cancer among all head and neck cancers. Deregulation of epigenetic effectors, including altered expression of histone methyltransferases from the MLL (mixed lineage leukemia) family, have been reported in many cancer types, yet little is known concerning their involvement in larynx cancer. Our objective was to determine the expression profile of MLL genes in larynx carcinoma and normal adjacent tissues and correlate this profile to tumor characteristics. We analyzed the expression profile of 5 MLL genes in 13 cases of larynx carcinoma and their adjacent non-tumor tissues using quantitative real-time PCR. MLL3 was significantly downregulated in tumor samples compared to their normal counterparts, and all MLL genes showed decreased expression in advanced tumors compared to tumors in the initial stage. Altered expression in a single MLL gene was associated with a similar alteration in the other MLL genes, revealing a strong correlation of expression in each individual patient. In conclusion, MLL genes may have similar transcriptional control, and decreased expression of these genes may contribute to larynx cancer progression.
authors: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil.
authors: David Livingstone Alves
authors: DL
authors: Figueiredo
authors: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil.
authors: Doralina
authors: D
authors: do Amaral Rabello
authors: Center for Cell Therapy and Regional Blood Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
authors: Dalila Luciola
authors: DL
authors: Zanette
authors: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
authors: Fabiano Pinto
authors: FP
authors: Saggioro
authors: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
authors: Rui Celso Martins
authors: RC
authors: Mamede
authors: Center for Cell Therapy and Regional Blood Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
authors: Marco Antonio
authors: MA
authors: Zago
authors: Center for Cell Therapy and Regional Blood Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
authors: Wilson Araújo
authors: WA
authors: da Silva
authors: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil.
authors: Felipe
authors: F
authors: Saldanha-Araújo
authors: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil.
authors: Fabio
authors: F
authors: Pittella Silva
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008780:Methyltransferases / Q000235:genetics*; D008875:Middle Aged; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C497422:KMT2A protein, human; D051788:Myeloid-Lymphoid Leukemia Protein; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase