title: Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco.
abstract: EaCHS1 functions in the tolerance of plantlets to salinity stress by maintaining ROS homeostasis. Chalcone synthase (CHS) is an essential enzyme in the biosynthesis of flavonoids. Expression of CHS is governed by a wide range of environmental stimuli, including UV light, pathogen attack, and circadian clocks. However, little research exists on the relationship between CHS and salinity stress. In this work, we constructed separate overexpression and RNA interference vectors of EaCHS1, and transferred them into tobacco. Overexpression of EaCHS1 increased the production of downstream flavonoids and the expressions of related genes in the phenylpropanoid pathway. It also improved resistance to salinity stress during seed germination and root development. In contrast, heterologous silencing of endogenous CHS in tobacco by a conserved EaCHS1 fragment had opposite effect. Together, our results indicated that changing the expression level of EaCHS1 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to salinity stress by maintaining ROS homeostasis.
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Chen
authors: C
authors: Lijuan
authors: Guo
authors: G
authors: Huiming
authors: Lin
authors: L
authors: Yi
authors: Cheng
authors: C
authors: Hongmei
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D036603:Ageratina / Q000201:enzymology* / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055049:Salt Tolerance; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000502:physiology
chemical_list: D005419:Flavonoids; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D000217:Acyltransferases; C020141:flavanone synthetase
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