title: A peroxisomal APX from Puccinellia tenuiflora improves the abiotic stress tolerance of transgenic Arabidopsis thaliana through decreasing of H2O2 accumulation.
abstract: Ascorbate peroxidase (APX, EC 1.11.1.11) is one of the major members of the ROS scavenging system that plays an important role in improving saline-alkali tolerance. Puccinellia tenuiflora, as a perennial wild grass, is able to grow in extreme saline-alkali soil environments. In this study, we investigated the relationship between the P. tenuiflora ascorbate peroxidase (PutAPX) gene and saline-alkali tolerance. A phylogenetic analysis indicated that PutAPX is closely related to AtAPX3 and OsAPX4 and that these genes are on the same branch. The PutAPX-GFP fusion protein is located in the peroxisome in onion epidermal cells. The transcriptional expression of PutAPX increased with prolonged exposure to NaCl, NaHCO3, PEG6000 and H2O2 stresses in P. tenuiflora. The overexpression of PutAPX in Arabidopsis thaliana significantly increased the tolerance of plants treated with 150 and 175mM NaCl and decreased the extent of lipid peroxidation. The transgenic seedlings presented higher chlorophyll content than wild type (WT) seedlings treated with 1, 3, and 5mM NaHCO3 and 3mM H2O2. The DAB staining results revealed that the H2O2 content in transgenic seedlings was significantly lower than that in WT plants under both normal conditions and 200mM NaCl stress. Moreover, the expression of APX proteins and enzyme activity in the transgenic seedlings increased to level that were greater than twofold higher than those found in WT plants exposed to 200mM NaCl. The saline-alkali tolerance conferred by the PutAPX gene may provide a reliable basis for the use of molecular breeding techniques to improve plant tolerance and obtain a better understanding of the physiological mechanism of anti-oxidative and ROS stresses.
authors: Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, No. 26 Hexing Road, Nangang District, Harbin 150040, China; Laboratory of Soybean Molecular Biology and Molecular Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, No. 138 Haping Road, Nangang District, Harbin 150081, China.
authors: Qingjie
authors: Q
authors: Guan
authors: Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, No. 26 Hexing Road, Nangang District, Harbin 150040, China.
authors: Zhenjuan
authors: Z
authors: Wang
authors: Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, No. 26 Hexing Road, Nangang District, Harbin 150040, China.
authors: Xuhui
authors: X
authors: Wang
authors: Asian Natural Environmental Science Center, The University of Tokyo, Nishitokyo, Tokyo 188-0002, Japan.
authors: Tetsuo
authors: T
authors: Takano
authors: Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, No. 26 Hexing Road, Nangang District, Harbin 150040, China. Electronic address: shenkuiliu@nefu.edu.cn.
authors: Shenkui
authors: S
authors: Liu
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation; D020675:Peroxisomes / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000201:enzymology* / Q000235:genetics; D055049:Salt Tolerance; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases
keywords: APX (ascorbate peroxidase); Arabidopsis thaliana; Puccinellia tenuiflora; Salt-alkali and oxidative stresses; Transgenic plant