title: Polarity protein Par3 controls B-cell receptor dynamics and antigen extraction at the immune synapse.
abstract: B-cell receptor (BCR) engagement with surface-tethered antigens leads to the formation of an immune synapse, which facilitates antigen uptake for presentation to T-lymphocytes. Antigen internalization and processing rely on the early dynein-dependent transport of BCR-antigen microclusters to the synapse center, as well as on the later polarization of the microtubule-organizing center (MTOC). MTOC repositioning allows the release of proteases and the delivery of MHC class II molecules at the synapse. Whether and how these events are coordinated have not been addressed. Here we show that the ancestral polarity protein Par3 promotes BCR-antigen microcluster gathering, as well as MTOC polarization and lysosome exocytosis, at the synapse by facilitating local dynein recruitment. Par3 is also required for antigen presentation to T-lymphocytes. Par3 therefore emerges as a key molecule in the coupling of the early and late events needed for efficient extraction and processing of immobilized antigen by B-cells.
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Anne
authors: A
authors: Reversat
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France Departamento de Biologia Celular y Molecular, Pontificia Universidad Catolica de Chile, 6513677 Santiago, Chile.
authors: Maria-Isabel
authors: MI
authors: Yuseff
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Danielle
authors: D
authors: Lankar
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Odile
authors: O
authors: Malbec
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Dorian
authors: D
authors: Obino
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Mathieu
authors: M
authors: Maurin
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Naga Venkata Gayathri
authors: NV
authors: Penmatcha
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France Facultad de Ciencias de la Salud, Universidad San Sebastián, 7510157 Santiago, Chile.
authors: Alejandro
authors: A
authors: Amoroso
authors: Cell and Tissue Imaging Core Facility (PICT-IBiSA) and Nikon Imaging Centre, Institut Curie, UMR144, Centre de Recherche, 75005 Paris, France.
authors: Lucie
authors: L
authors: Sengmanivong
authors: Department of Pathology and Cell Biology, Columbia University, New York, NY 10032.
authors: Gregg G
authors: GG
authors: Gundersen
authors: Genentech, San Francisco, CA 94080.
authors: Ira
authors: I
authors: Mellman
authors: Université Paris Descartes, Sorbonne Paris Cité, CNRS UMR8250, 75270 Paris Cedex 06, France.
authors: François
authors: F
authors: Darchen
authors: Université Paris Descartes, Sorbonne Paris Cité, CNRS UMR8250, 75270 Paris Cedex 06, France.
authors: Claire
authors: C
authors: Desnos
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Paolo
authors: P
authors: Pierobon
authors: INSERM U932, Institut Curie, Centre de Recherche, 75005 Paris, France.
authors: Ana-Maria
authors: AM
authors: Lennon-Duménil
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000502:physiology*; D018797:Cell Cycle Proteins; D054992:Immunological Synapses / Q000276:immunology*; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000941:Antigens; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; C115084:Pard3 protein, mouse; D011947:Receptors, Antigen, B-Cell
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