title: Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination.
abstract: In nature, plants are often exposed to combinations of different stresses at the same time, while in many laboratory studies of molecular stress induction phenomena, single stress responses are analyzed. This study aims to identify the common (i.e. more general stress-responsive) and the stress-specific adjustments of the leaf proteome of wild barley to two often co-occurring stress phenomena, i.e. in response to (long-term) drought acclimation (DA) or to (transient) heat stress (HS). In addition, we analyzed those alterations which are specific for the combination of both stresses. Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry with a 1.5 threshold value of changes in relative protein contents. DA resulted in specific upregulation of proteins with cell detoxification functions, water homeostasis maintenance, amino acids synthesis and lipid metabolism and distinct forms of heat shock proteins (HSPs) and proteins with chaperon functions while proteins related to nitrogen metabolism were downregulated. This response was distinguished from the response to transient HS, which included upregulation of a broad range of HSP products. The common response to both stressors revealed upregulation of additional forms of HSPs and the downregulation of enzymes of the photosynthetic apparatus and chlorophyll binding proteins. The simultaneous exposure to both stress conditions resulted mostly in a combination of both stress responses and to unique abundance changes of proteins with yet unclear functions.
authors: Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue Str. 13, 60438, Frankfurt am Main, Germany, ashoub@em.uni-frankfurt.de.
authors: Ahmed
authors: A
authors: Ashoub
authors: Marion
authors: M
authors: Baeumlisberger
authors: Moritz
authors: M
authors: Neupaertl
authors: Michael
authors: M
authors: Karas
authors: Wolfgang
authors: W
authors: Brüggemann
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism* / Q000502:physiology*; D006358:Hot Temperature; D018515:Plant Leaves / Q000378:metabolism* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins
references: J Exp Bot. 2006;57(7):1537-46
references: 16574749
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Annu Rev Biochem. 2001;70:603-47
references: 11395418
references: Proteomics. 2005 Nov;5(16):4185-96
references: 16254930
references: J Biomol Tech. 2009 Dec;20(5):293-6
references: 19949705
references: Planta. 2013 Mar;237(3):771-81
references: 23129216
references: Amino Acids. 2013 Feb;44(2):345-59
references: 22707152
references: Plant Physiol. 1998 Feb 1;116(2):539-46
references: 9490757
references: Curr Protein Pept Sci. 2001 Sep;2(3):205-25
references: 12369933
references: J Exp Bot. 2014 Mar;65(5):1241-57
references: 24368505
references: Proteomics. 2005 Jan;5(1):235-44
references: 15672456
references: Proteomics. 2004 Jul;4(7):1873-82
references: 15221744
references: Trends Plant Sci. 2004 May;9(5):244-52
references: 15130550
references: J Exp Bot. 2009;60(12):3531-44
references: 19561048
references: Plant Physiol. 2013 Apr;161(4):2159-70
references: 23444343
references: J Exp Bot. 2013 Aug;64(11):3201-12
references: 23918963
references: Methods Mol Biol. 2007;355:1-8
references: 17093296
references: Arch Biochem Biophys. 1978 May;188(1):206-13
references: 209742
references: Plant J. 2002 Aug;31(3):279-92
references: 12164808
references: Nature. 1996 Jun 13;381(6583):571-9
references: 8637592
references: Science. 2003 Dec 12;302(5652):1917-9
references: 14671289
references: Plant Physiol. 2001 Apr;125(4):1912-8
references: 11299370
references: Plant Physiol. 2004 Apr;134(4):1683-96
references: 15047901
references: Mol Biol Rep. 2012 May;39(5):6387-97
references: 22297690
references: New Phytol. 2009;182(3):608-20
references: 19422547
references: J Exp Bot. 2000 Jan;51(342):9-17
references: 10938791
references: Plant Mol Biol. 2013 Sep;83(1-2):89-103
references: 23443681
references: Plant Physiol. 1974 Feb;53(2):258-60
references: 16658686
references: Science. 1966 Sep 2;153(3740):1074-80
references: 17737582
references: Curr Opin Plant Biol. 2005 Feb;8(1):86-92
references: 15653405
references: J Proteome Res. 2009 Nov;8(11):5317-24
references: 19725589
references: Funct Integr Genomics. 2008 Nov;8(4):387-405
references: 18512091
references: J Exp Bot. 2006;57(15):4003-13
references: 17075075
references: Nat Protoc. 2006;1(4):1852-8
references: 17487168
references: Plant Physiol. 2002 Nov;130(3):1143-51
references: 12427981
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Mol Syst Biol. 2012;8:606
references: 22929616
references: J Biol Chem. 1975 May 25;250(10):4007-21
references: 236308
references: New Phytol. 2014 Jul;203(1):32-43
references: 24720847
references: Planta. 2003 Nov;218(1):1-14
references: 14513379
references: Funct Integr Genomics. 2014 Mar;14(1):135-48
references: 24122211
references: Electrophoresis. 1988 Jun;9(6):255-62
references: 2466658