title: Surface Enzyme Chemistries for Ultrasensitive Microarray Biosensing with SPR Imaging.
abstract: The sensitivity and selectivity of surface plasmon resonance imaging (SPRI) biosensing with nucleic acid microarrays can be greatly enhanced by exploiting various nucleic acid ligases, nucleases, and polymerases that manipulate the surface-bound DNA and RNA. We describe here various examples from each of these different classes of surface enzyme chemistries that have been incorporated into novel detection strategies that either drastically enhance the sensitivity of or create uniquely selective methods for the SPRI biosensing of proteins and nucleic acids. A dual-element generator-detector microarray approach that couples a bioaffinity adsorption event on one microarray element to nanoparticle-enhanced SPRI measurements of nucleic acid hybridization adsorption on a different microarray element is used to quantitatively detect DNA, RNA, and proteins at femtomolar concentrations. Additionally, this dual-element format can be combined with the transcription and translation of RNA from surface-bound double-stranded DNA (dsDNA) templates for the on-chip multiplexed biosynthesis of aptamer and protein microarrays in a microfluidic format; these microarrays can be immediately used for real-time SPRI bioaffinity sensing measurements.
authors: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors: Jennifer B
authors: JB
authors: Fasoli
authors: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors: Robert M
authors: RM
authors: Corn
mesh_terms: D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D009696:Nucleic Acids / Q000032:analysis; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D021881:DNA, Catalytic; D009696:Nucleic Acids; D011506:Proteins; D004259:DNA-Directed DNA Polymerase; D008025:Ligases
references: Nat Rev Genet. 2004 Jul;5(7):522-31
references: 15211354
references: Phys Chem Chem Phys. 2011 Jun 28;13(24):11551-67
references: 21509385
references: Acc Chem Res. 2011 Dec 20;44(12):1349-58
references: 21819040
references: Anal Chem. 2004 Oct 1;76(19):5677-84
references: 15456285
references: Chem Biol. 1994 Dec;1(4):223-9
references: 9383394
references: J Am Chem Soc. 2002 Sep 4;124(35):10596-604
references: 12197762
references: Science. 2004 Jul 2;305(5680):86-90
references: 15232106
references: J Am Chem Soc. 2006 Nov 1;128(43):14044-6
references: 17061884
references: Anal Chem. 2004 Dec 15;76(24):7257-62
references: 15595867
references: Langmuir. 2006 Jun 6;22(12):5241-50
references: 16732647
references: J Am Chem Soc. 2012 Aug 1;134(30):12358-61
references: 22793370
references: Angew Chem Int Ed Engl. 2008;47(34):6330-7
references: 18680110
references: Nat Rev Cancer. 2003 Apr;3(4):267-75
references: 12671665
references: Genome Biol. 2001;2(2):RESEARCH0004
references: 11182887
references: Langmuir. 2012 Jun 5;28(22):8281-5
references: 22458258
references: Biosens Bioelectron. 2011 Jan 15;26(5):2053-9
references: 20926281
references: Curr Opin Chem Eng. 2014 May 1;4:79-87
references: 24791224
references: Lab Chip. 2010 Mar 7;10(5):581-8
references: 20162233
references: Annu Rev Biochem. 1999;68:611-47
references: 10872462
references: RNA. 2006 May;12(5):913-20
references: 16540696
references: Anal Chem. 2007 Feb 1;79(3):1082-8
references: 17263339
references: Science. 1990 Aug 3;249(4968):505-10
references: 2200121
references: Anal Chem. 2011 May 15;83(10):3897-902
references: 21524060
references: Anal Chem. 1998 Dec 15;70(24):5177-83
references: 9868916
references: Anal Bioanal Chem. 2008 Jul;391(5):1509-19
references: 18265962
references: Anal Chem. 2005 Aug 15;77(16):5096-100
references: 16097744
references: Nucleic Acids Res. 2005;33(19):6151-63
references: 16286368
references: J Am Chem Soc. 2007 May 9;129(18):5804-5
references: 17432859
references: Anal Bioanal Chem. 2007 Mar;387(5):1831-40
references: 17203259
references: Biosens Bioelectron. 2010 Oct 15;26(2):575-9
references: 20692147
references: Nucleic Acids Res. 2006;34(22):6416-24
references: 17130155
references: Nanoscale. 2011 Dec;3(12):5067-75
references: 22037864
references: J Immunol Methods. 2005 Feb;297(1-2):125-32
references: 15777936
references: Annu Rev Biochem. 2005;74:53-82
references: 15952881
references: Nano Lett. 2010 Jul 14;10(7):2549-54
references: 20509678
references: Annu Rev Phys Chem. 2000;51:41-63
references: 11031275
references: Anal Chem. 2007 Dec 1;79(23):8994-9
references: 17963362
references: Nat Genet. 1999 Jan;21(1 Suppl):10-4
references: 9915494
references: J Am Chem Soc. 2013 Feb 13;135(6):2072-5
references: 23351044
references: Anal Chem. 2006 May 1;78(9):3158-64
references: 16643008
references: Anal Chem. 2001 Nov 15;73(22):5525-31
references: 11816583
references: J Biotechnol. 2000 Mar;74(1):5-13
references: 10943568
references: Anal Chem. 2004 Nov 1;76(21):6173-8
references: 15516107
references: Nat Rev Cancer. 2006 Nov;6(11):857-66
references: 17060945
references: Nat Genet. 2005 May;37(5):549-54
references: 15838508
references: Nat Rev Drug Discov. 2006 Apr;5(4):310-20
references: 16582876
references: Science. 2000 Sep 8;289(5485):1757-60
references: 10976070
references: Nat Methods. 2008 Feb;5(2):175-7
references: 18204456
references: J Am Chem Soc. 2011 Mar 30;133(12):4271-3
references: 21391582
references: Anal Chem. 2012 Jan 17;84(2):793-821
references: 22111937
references: Langmuir. 2011 May 17;27(10):6534-40
references: 21488682
references: J Phys Chem Lett. 2012 Sep 20;3(18):2682-91
references: 26295892
references: Genes Dev. 1997 Nov 1;11(21):2755-66
references: 9353246
references: J Am Chem Soc. 2010 Jul 14;132(27):9265-7
references: 20565098
references: Analyst. 2011 Feb 7;136(3):436-47
references: 21049107
references: J Am Chem Soc. 2004 Apr 7;126(13):4086-7
references: 15053580
references: Langmuir. 2005 Apr 26;21(9):4050-7
references: 15835973
references: Nature. 2000 Jan 13;403(6766):175-9
references: 10646598
references: Nucleic Acids Res. 1987 Nov 11;15(21):8783-98
references: 3684574
references: Clin Chem. 1999 Sep;45(9):1628-50
references: 10471678
references: Anal Chem. 2012 Jan 3;84(1):440-5
references: 22126812
references: Nature. 1990 Aug 30;346(6287):818-22
references: 1697402
references: Anal Chem. 2005 Dec 1;77(23):7832-7
references: 16316195