title: Cadmium accumulation and metallothionein biosynthesis in cadmium-treated freshwater mussel Anodonta woodiana.
abstract: This study investigated the distribution of cadmium (Cd) and the protein level of metallothionein (MT) and examined the relationship of Cd accumulation and the MT concentration in different tissues of freshwater mussel Anodonta woodiana following Cd treatment. The mussels were exposed to Cd (4.21, 8.43, 16.86, 33.72 and 67.45 mg L-1) for 24, 48, 72 and 96 h, respectively. After Cd treatment, the gills, mantle, foot, visceral mass and digestive gland tissues were collected for analysis. We found that, in the controls, Cd distributed in all tissues in the concentration order of gills>mantle>foot>visceral mass>digestive gland. Upon Cd treatment, Cd concentration significantly increased in all tissues. The highest Cd accumulation was found in the digestive gland, which was 0.142 mg g-1 (P<0.05). MT levels in the gills and mantle of the mussels increased significantly (P<0.05), which were in positive correlation with Cd accumulation in the tissues (P<0.05). In conclusion, our results demonstrated a correlation between Cd accumulation and MT up-regulation in gills and mantle of the mussels after Cd treatment. It is suggested that the protein level of MT in gills and mantle of Anodonta woodiana is a good biomarker for Cd contamination.
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Yongquan
authors: Y
authors: Li
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Huizhen
authors: H
authors: Yang
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Na
authors: N
authors: Liu
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Jixian
authors: J
authors: Luo
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Qian
authors: Q
authors: Wang
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: Lan
authors: L
authors: Wang
mesh_terms: D000818:Animals; D049877:Anodonta / Q000737:chemistry / Q000378:metabolism*; D002104:Cadmium / Q000032:analysis / Q000378:metabolism*; D004784:Environmental Monitoring; D008668:Metallothionein / Q000032:analysis / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D008668:Metallothionein
references: Environ Toxicol. 2008 Jun;23(3):393-400
references: 18214890
references: Mar Environ Res. 2002 Apr;53(3):227-41
references: 11939291
references: Biochimie. 2006 Nov;88(11):1787-92
references: 16935407
references: Environ Pollut. 2003;125(3):301-4
references: 12826407
references: Environ Monit Assess. 2012 Apr;184(4):2177-92
references: 21611846
references: Comp Biochem Physiol C Toxicol Pharmacol. 2001 Apr;128(4):479-93
references: 11301290
references: Aquat Toxicol. 2012 Apr;110-111:208-13
references: 22343466
references: Biometals. 2006 Aug;19(4):399-407
references: 16841249
references: Arch Environ Contam Toxicol. 2003 Nov;45(4):468-78
references: 14708663
references: PLoS One. 2013 Nov 18;8(11):e80475
references: 24260400
references: Environ Pollut. 2002;118(1):5-17
references: 11996382
references: Toxicol Appl Pharmacol. 2007 Apr 15;220(2):186-96
references: 17350662
references: Aquat Toxicol. 2006 Feb 10;76(2):160-202
references: 16289342
references: Histochem J. 1990 Apr;22(4):187-91
references: 2201669
references: Toxicol Appl Pharmacol. 1982 Apr;63(2):270-4
references: 7089976
references: Mutat Res. 2003 Dec 10;533(1-2):211-26
references: 14643422
references: Ecotoxicol Environ Saf. 2010 Mar;73(3):306-11
references: 19913912
references: Cell Mol Biol (Noisy-le-grand). 2000 Mar;46(2):311-30
references: 10774922
references: Nucleic Acids Res. 1994 Nov 25;22(23):5016-23
references: 7800494
references: Ecotoxicol Environ Saf. 2005 Sep;62(1):140-3
references: 15978300
references: Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jan;140(1):39-45
references: 15792621
references: Comp Biochem Physiol B Biochem Mol Biol. 1997 Jan;116(1):75-86
references: 9080664
references: Arch Environ Contam Toxicol. 2009 May;56(4):747-53
references: 18846314
references: Nature. 1985 Oct 31-Nov 6;317(6040):828-31
references: 4058587
references: Comp Biochem Physiol C Toxicol Pharmacol. 2009 Aug;150(2):186-95
references: 19409510
references: Int J Older People Nurs. 2010 Mar;5(1):51-60
references: 20925757
references: Environ Toxicol. 2002;17(3):170-86
references: 12112625
references: Sci Total Environ. 2000 Mar 20;247(2-3):295-311
references: 10803557
references: Cell Mol Biol (Noisy-le-grand). 2000 Mar;46(2):295-309
references: 10774921
references: Ecotoxicol Environ Saf. 1997 Nov;38(2):122-31
references: 9417853
references: Annu Rev Biochem. 1986;55:913-51
references: 3527054
references: Biochem Pharmacol. 2000 Jan 1;59(1):95-104
references: 10605938
references: Biomarkers. 2001;6(2):91-107
references: 23886106
references: PLoS One. 2013 May 30;8(5):e64020
references: 23737962
references: Comp Biochem Physiol C Toxicol Pharmacol. 2005 Mar-Apr;140(3-4):383-94
references: 15925547
references: Comp Biochem Physiol C Toxicol Pharmacol. 2007 Apr;145(3):413-9
references: 17324630
references: Sci Total Environ. 2008 Jan 15;389(1):37-45
references: 17910979
references: Proc Natl Acad Sci U S A. 1984 Dec;81(23):7318-22
references: 6095286
references: Biomarkers. 2005 Sep-Oct;10(5):342-59
references: 16243720
references: Environ Res. 2005 Jun;98(2):210-4
references: 15820727
references: Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1219-23
references: 8108390
references: Aquat Toxicol. 2005 Mar 25;72(1-2):83-97
references: 15748749