title: Vascularized versus nonvascularized facial nerve grafts using a new rabbit model.
abstract: BACKGROUND
  The use of vascularized nerve graft models has been limited because of the complexity of the operation. The authors sought to develop a simple and effective rabbit model for facial nerve repair and evaluated its advantages over conventional nerve grafts.
  
  
  METHODS
  Rabbits were divided into three groups consisting of six rabbits each. The central auricular nerve and its nutrient vessels were used as a vascularized graft. Rabbits were grafted with a vascularized facial nerve graft (vascularized nerve graft group), with a free nerve graft (free nerve graft group), or with a vascularized nerve graft and a free nerve graft on each side of the face (vascularized nerve graft/free nerve graft group). Four months after surgery, facial performance and electrophysiologic monitoring were evaluated. The rabbits were then killed to prepare the nerve specimens for histologic, immunohistochemical, and transmission electron microscope study.
  
  
  RESULTS
  At 4 months after the facial nerve repair, the functional recovery of the facial nerve was observed and analyzed. The side grafted with vascularized nerve graft was superior to the side grafted with free nerve graft. Regenerated nerve fibers were observed in all groups, and rabbits grafted with vascularized nerve grafts had more regenerated axons than those that underwent free nerve grafting, although the regenerated nerves were not as good as the natural nerves.
  
  
  CONCLUSIONS
  This study demonstrates that it is feasible to establish a vascularized nerve graft model in rabbits. The model offers the obvious advantages of operability and reliability. The vascularized nerve graft is demonstrated to have a superior value for facial nerve repair.
authors: Shanghai, People's Republic of China From the Departments of Oral and Maxillofacial Head and Neck Oncology and Oral Pathology, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology.
authors: Yun
authors: Y
authors: Zhu
authors: Shengwen
authors: S
authors: Liu
authors: Shanghui
authors: S
authors: Zhou
authors: Zhiwei
authors: Z
authors: Yu
authors: Zhen
authors: Z
authors: Tian
authors: Chenping
authors: C
authors: Zhang
authors: Wenjun
authors: W
authors: Yang
mesh_terms: D000818:Animals; D005146:Facial Asymmetry / Q000517:prevention & control; D005154:Facial Nerve / Q000098:blood supply / Q000502:physiology / Q000601:surgery* / Q000648:ultrastructure; D008854:Microscopy, Electron; D023421:Models, Animal; D009416:Nerve Regeneration; D016067:Nerve Transfer / Q000379:methods*; D009431:Neural Conduction; D011183:Postoperative Complications / Q000517:prevention & control; D011817:Rabbits / Q000601:surgery*; D011897:Random Allocation; D020127:Recovery of Function; D012583:Schwann Cells / Q000648:ultrastructure
references: Schultes G, Gaggl A, Kleinert R, Kärcher H. Vascularized versus non-vascularized nerve transfers: Histologic study in rats. J Reconstr Microsurg. 2001;17:637–642
references: Taylor GI, Ham FJ. The free vascularized nerve graft: A further experimental and clinical application of microvascular techniques. Plast Reconstr Surg. 1976;57:413–426
references: Ozcan G, Shenaq S, Spira M. Vascularized nerve tube: An experimental alternative for vascularized nerve grafts over short gaps. J Reconstr Microsurg. 1993;9:405–413
references: Lux P, Breidenbach W, Firrell J. Determination of temporal changes in blood flow in vascularized and nonvascularized nerve grafts in the dog. Plast Reconstr Surg. 1988;82:133–144
references: Koshima I, Harii K. Experimental study of vascularized nerve grafts: Multifactorial analyses of axonal regeneration of nerves transplanted into an acute burn wound. J Hand Surg Am. 1985;10:64–72
references: Kimata Y, Sakuraba M, Hishinuma S, Ebihara S, Hayashi R, Asakage T. Free vascularized nerve grafting for immediate facial nerve reconstruction. Laryngoscope. 2005;115:331–336
references: Shibata M, Tsai TM, Firrell J, Breidenbach WC. Experimental comparison of vascularized and nonvascularized nerve grafting. J Hand Surg Am. 1988;13:358–365
references: Tark KC, Roh TS. Morphometric study of regeneration through vascularized nerve graft in a rabbit sciatic nerve model. J Reconstr Microsurg. 2001;17:109–114
references: Ozcan G, Shenaq S, Spira M. A new vascularized nerve graft model in the rabbit. J Reconstr Microsurg. 1992;8:35–40
references: Yian CH, Paniello RC, Gershon Spector J. Inhibition of motor nerve regeneration in a rabbit facial nerve model. Laryngoscope. 2001;111:786–791
references: Midrio M. The denervated muscle: Facts and hypotheses. A historical review. Eur J Appl Physiol. 2006;98:1–21
references: Iwai M, Tamai S, Yajima H, Kawanishi K. Experimental study of vascularized nerve graft: Evaluation of nerve regeneration using choline acetyltransferase activity. Microsurgery. 2001;21:43–51
references: Bertelli JA, Taleb M, Mira JC, Calixto JB. Muscle fiber type reorganization and behavioral functional recovery of rat median nerve repair with vascularized or conventional nerve grafts. Restor Neurol Neurosci. 1996;10:5–12
references: Ozcan G, Shenaq S, Mirabi B, Spira M. Nerve regeneration in a bony bed: Vascularized versus nonvascularized nerve grafts. Plast Reconstr Surg. 1993;91:1322–1331
references: Jeannon JP, Calman F, Gleeson M, et al. Management of advanced parotid cancer: A systematic review. Eur J Surg Oncol. 2009;35:908–915
references: Spiro RH. Salivary neoplasms: Overview of a 35-year experience with 2,807 patients. Head Neck Surg. 1986;8:177–184
references: Kimata Y, Sakuraba M, Hishinuma S, Ebihara S, Hayashi R, Asakage T. Free vascularized nerve grafting for immediate facial nerve reconstruction. Laryngoscope. 2005;115:331–336