title: Simultaneous characterization of instantaneous Young's modulus and specific membrane capacitance of single cells using a microfluidic system.
abstract: This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young's modulus (E(instantaneous)) and specific membrane capacitance (C(specific membrane)) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (L(instantaneous)) and transitional aspiration length (L(transitional)), which was further translated to E(instantaneous). An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine C(specific membrane). A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing E(instantaneous) of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular E(instantaneous) and C(specific membrane), this approach can facilitate a more comprehensive understanding of cellular biophysical properties.
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. gaochunzy@gmail.com.
authors: Yang
authors: Y
authors: Zhao
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. dychen@mail.ie.ac.cn.
authors: Deyong
authors: D
authors: Chen
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. luoyana88@126.com.
authors: Yana
authors: Y
authors: Luo
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. ccmucf@aliyun.com.
authors: Feng
authors: F
authors: Chen
authors: Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China. zhao_xiaoting@126.com.
authors: Xiaoting
authors: X
authors: Zhao
authors: Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China. jmcyf627@sina.com.
authors: Mei
authors: M
authors: Jiang
authors: Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China. yuewentao@gmail.com.
authors: Wentao
authors: W
authors: Yue
authors: Department of Mechanical Engineering, University of Alberta, Edmonton, AB T6G 2G8, Canada. rlong2@ualberta.ca.
authors: Rong
authors: R
authors: Long
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. jbwang@mail.ie.ac.cn.
authors: Junbo
authors: J
authors: Wang
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. chenjian@mail.ie.ac.cn.
authors: Jian
authors: J
authors: Chen
mesh_terms: D015374:Biosensing Techniques; D002462:Cell Membrane / Q000737:chemistry; D017097:Electric Impedance; D006801:Humans; D046210:Microfluidic Analytical Techniques; D059010:Single-Cell Analysis
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