title: Histone acetyltransferase p300/CBP-associated factor is an effective suppressor of secretory immunoglobulin synthesis in immature B cells.
abstract: The histone acetyltransferase p300/CBP-associated factor (PCAF) catalyzes acetylation of core histones and plays important roles in epigenetics by altering the chromatin structure in vertebrates. In this study, PCAF-deficient DT40 mutants were analyzed and it was found that PCAF participates in regulation of secretory IgM heavy chain (H-chain) synthesis. Remarkably, PCAF-deficiency causes an increase in the amount of secretory IgM H-chain mRNA, but not in that of IgM light chain and membrane-bound IgM H-chain mRNAs, resulting in dramatic up-regulation of the amount of secretory IgM protein. These findings suggest that PCAF regulates soluble antibody production and is thus an effective suppressor of secretory IgM H-chain synthesis.
authors: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki, 889-1692; Division of Bacteriology, Department of Infectious Diseases Control, International Research Center for Infectious Diseases.
authors: Hidehiko
authors: H
authors: Kikuchi
authors: Masami
authors: M
authors: Nakayama
authors: Chikage
authors: C
authors: Kawai
authors: Futoshi
authors: F
authors: Kuribayashi
authors: Hitomi
authors: H
authors: Mimuro
authors: Shinobu
authors: S
authors: Imajoh-Ohmi
authors: Hideki
authors: H
authors: Nishitoh
authors: Yasunari
authors: Y
authors: Takami
authors: Tatsuo
authors: T
authors: Nakayama
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D015536:Down-Regulation; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000235:genetics; D054448:Precursor Cells, B-Lymphoid / Q000201:enzymology / Q000378:metabolism*; D050880:p300-CBP Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C033027:secretory IgM
keywords: epigenetic regulation; histone acetyltransferase; immunoglobulin; p300/CBP-associated factor