title: Early Detection of Lung Cancer in Serum by a Panel of MicroRNA Biomarkers.
abstract: BACKGROUND
  The objective of the study was to develop a panel of microRNAs (miRNAs) as highly sensitive and specific biomarkers for lung cancer early detection.
  
  
  METHODS
  The study contained 2 phases: first, preliminary marker selection based on previous reports on the serum of 24 early stage lung cancer patients and 24 healthy control subjects by TaqMan probe-based real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR); and second, validation of miRNA markers on 94 early stage lung cancer, 48 stage III to IV lung cancer, and 111 healthy control serum samples.
  
  
  RESULTS
  A total of 3 miRNAs (miR-125a-5p, miR-25, and miR-126) were selected for further analysis in this study. The combination of the 3 miRNAs could produce 0.936 area under the receiver operating characteristic curve value in distinguishing early stage lung cancer patients from control subjects with 87.5% sensitivity and 87.5% specificity, respectively. The diagnostic value of the miRNA panel in an independent set of lung cancer patients confirmed the sensitivity and specificity.
  
  
  CONCLUSIONS
  The results demonstrated that the panel of miRNA biomarkers had the potential for the early detection of lung cancer.
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China; University of Chinese Academy of Sciences, Beijing, China.
authors: Ping
authors: P
authors: Wang
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Dawei
authors: D
authors: Yang
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China.
authors: Honglian
authors: H
authors: Zhang
authors: Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Xuyu
authors: X
authors: Wei
authors: Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Tianle
authors: T
authors: Ma
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China; University of Chinese Academy of Sciences, Beijing, China.
authors: Zule
authors: Z
authors: Cheng
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Qunying
authors: Q
authors: Hong
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Jie
authors: J
authors: Hu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Hanjing
authors: H
authors: Zhuo
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Yuanlin
authors: Y
authors: Song
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China.
authors: Chunping
authors: C
authors: Jia
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China.
authors: Fengxiang
authors: F
authors: Jing
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China.
authors: Qinghui
authors: Q
authors: Jin
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Chunxue
authors: C
authors: Bai
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China. Electronic address: hjmao@mail.sim.ac.cn.
authors: Hongju
authors: H
authors: Mao
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, China.
authors: Jianlong
authors: J
authors: Zhao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarker; Early detection; Lung cancer; MicroRNA; Serum