title: Isolation and characterization of bacteria from the rhizosphere and bulk soil of Stellera chamaejasme L.
abstract: This study is the first to describe the composition and characteristics of culturable bacterial isolates from the rhizosphere and bulk soil of the medicinal plant Stellera chamaejasme L. at different growth stages. Using a cultivation-dependent approach, a total of 148 isolates showing different phenotypic properties were obtained from the rhizosphere and bulk soil. Firmicutes and Actinobacteria were the major bacterial groups in both the rhizosphere and bulk soil at all 4 growth stages of S. chamaejasme. The diversity of the bacterial community in the rhizosphere was higher than that in bulk soil in flowering and fruiting stages. The abundance of bacterial communities in the rhizosphere changed with the growth stages and had a major shift at the fruiting stage. Dynamic changes of bacterial abundance and many bacterial groups in the rhizosphere were similar to those in bulk soil. Furthermore, most bacterial isolates exhibited single or multiple biochemical activities associated with S. chamaejasme growth, which revealed that bacteria with multiple physiological functions were abundant and widespread in the rhizosphere and bulk soil. These results are essential (i) for understanding the ecological roles of bacteria in the rhizosphere and bulk soil and (ii) as a foundation for further evaluating their efficacy as effective S. chamaejasme growth-promoting rhizobacteria.
authors: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 18 Tianshui Middle Road, Lanzhou 730000, People's Republic of China., Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors: Haiyan
authors: H
authors: Cui
authors: Xiaoyan
authors: X
authors: Yang
authors: Dengxue
authors: D
authors: Lu
authors: Hui
authors: H
authors: Jin
authors: Zhiqiang
authors: Z
authors: Yan
authors: Jixiang
authors: J
authors: Chen
authors: Xiuzhuang
authors: X
authors: Li
authors: Bo
authors: B
authors: Qin
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D044822:Biodiversity; D008969:Molecular Sequence Data; D010802:Phylogeny; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D029645:Thymelaeaceae / Q000254:growth & development*
chemical_list: D012987:Soil
keywords: Stellera chamaejasme L.; communauté bactérienne cultivable; culturable bacterial community; plant growth promotion; rhizosphere and bulk soil; rhizosphère et sol en vrac; stimulation de la croissance végétale