title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii.
abstract: Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides.
authors: Central Laboratory Division-LIM03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil jnaj99@gmail.com.
authors: João
authors: J
authors: Nobrega de Almeida
authors: Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
authors: Gilda M B
authors: GM
authors: Del Negro
authors: Department of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Rafaella C
authors: RC
authors: Grenfell
authors: Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
authors: Monica S M
authors: MS
authors: Vidal
authors: Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
authors: Danilo Y
authors: DY
authors: Thomaz
authors: Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
authors: Dulce S Y
authors: DS
authors: de Figueiredo
authors: Department of Microbiology and Immunology, Instituto de Biociências de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, Brazil.
authors: Eduardo
authors: E
authors: Bagagli
authors: Department of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Luiz
authors: L
authors: Juliano
authors: Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
authors: Gil
authors: G
authors: Benard
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012331:RNA, Fungal / Q000235:genetics; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D018840:HSP70 Heat-Shock Proteins; D012331:RNA, Fungal
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