title: The respiratory syncytial virus fusion protein formulated with a novel combination adjuvant induces balanced immune responses in lambs with maternal antibodies.
abstract: Respiratory syncytial virus (RSV) causes severe respiratory illness in infants. There are no licensed vaccines to prevent RSV infection. The neonate receives short-term protection from maternally derived antibodies, which, however, can also interfere with the active response to vaccination. A RSV vaccine consisting of a truncated version of the fusion protein formulated with polyI:C, innate defense regulator peptide and polyphosphazene (ΔF/TriAdj), was evaluated in two to three week-old lambs. When delivered intrapulmonary, ΔF/TriAdj elicited IgA production in the lung in addition to a robust systemic response similar to that induced by intramuscular immunization. To investigate potential interference by maternal antibodies, pregnant ewes were vaccinated with ΔF/TriAdj. Lambs born to RSV F-immune or non-immune ewes were then given three vaccinations with ΔF/TriAdj at 3 days, 4 weeks and 8 weeks post-birth. Lambs immunized intramuscularly with ΔF/TriAdj vaccine developed high-affinity ΔF-specific serum IgG and virus neutralizing antibodies, and displayed an increase in the frequency of IFN-γ-secreting cells by in vitro restimulated peripheral blood mononuclear cells. Maternal antibodies did not interfere with the development of an immune response to ΔF/TriAdj in the newborn lambs. These results indicate that immunization of neonates with ΔF/TriAdj is effective even in the face of maternal antibodies.
authors: VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
authors: R
authors: R
authors: Garg
authors: VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
authors: L
authors: L
authors: Latimer
authors: VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
authors: V
authors: V
authors: Gerdts
authors: VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
authors: A
authors: A
authors: Potter
authors: VIDO-Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3; Microbiology & Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3. Electronic address: sylvia.vandenhurk@usask.ca.
authors: S
authors: S
authors: van Drunen Littel-van den Hurk
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007112:Immunity, Maternally-Acquired; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D011247:Pregnancy; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D034561:Sheep, Domestic / Q000276:immunology*; D014611:Vaccination; D014760:Viral Fusion Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: Animal model; Lambs; Maternal antibodies; Maternal immunization; Neonatal immunization; RSV