title: Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model.
abstract: OBJECTIVE
  HI-6 or asoxime in some sources is an antidotum for nerve agents. In recent experiments, implication of HI-6 in immunity response was proved; however, the issue was not studied in details. In this experiment, role of cytokines in HI-6 impact on immunity was searched. DESIG N: BALB/c mice were exposed to saline, HI-6 in a dose 1-100 mg/kg and/or 1 keyhole limpet hemocyanin (KLH) 1 mg/kg. Mice were sacrificed 21 days after experiment beginning and interleukins (IL) 1, 2, 4, 6 were determined by Enzyme Linked Immunosorbent Assay (ELISA).
  
  
  RESULTS
  The animals had no pathological manifestation. From the tested cytokines, no significant alteration was found for the IL-1, IL-4 and IL-6. IL-2 was significantly increased in a dose response manner.
  
  
  CONCLUSIONS
  The experimental data well correlates with the previous work where HI-6 caused increase of antibodies production. HI-6 is suitable to be used as an adjuvant whenever immunity should be pharmacologically altered.
authors: Miroslav
authors: M
authors: Pohanka
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006433:Hemocyanins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010091:Oximes / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D010091:Oximes; D011726:Pyridinium Compounds; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; C022870:asoxime chloride