title: Fabrication of hydroxyapatite on pure titanium by micro-arc oxidation coupled with microwave-hydrothermal treatment.
abstract: Porous hydroxyapatite (HA)-containing composite films were prepared by a novel method consisting of micro-arc oxidation (MAO) combined with microwave-hydrothermal (M-H) treatment. The morphology, composition and phase composition of the bioactive films were investigated with scanning electron microscopy with energy dispersive X-ray spectroscopy and X-ray diffraction. MTT assay was carried out to investigate the in vitro effects of the different surfaces on bone integration properties. The prepared MAO films consisted mainly of anatase, rutile and tricalcium phosphate along with amorphous calcium (Ca) and phosphorus (P) phases. The M-H-treated composite films were composed primarily of anatase, rutile and HA. As the time and temperature of the M-H treatment increased, the number of HA crystals gradually increased. Using the M-H method, HA was obtained at a lower temperature and in a shorter period of time compared to the conventional hydrothermal method. The results suggest that the M-H method significantly decreases the hydrothermal reaction temperature and also greatly shortens the reaction time. Due to the nanocrystallinity and porosity of the prepared composite films, the method presented here shows promise for the formation of bioactive materials for medical applications.
authors: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.
authors: Quan-ming
authors: QM
authors: Zhao
authors: Hui-lin
authors: HL
authors: Yang
authors: Zhong-tang
authors: ZT
authors: Liu
authors: Xiao-feng
authors: XF
authors: Gu
authors: Cheng
authors: C
authors: Li
authors: De-hong
authors: DH
authors: Feng
mesh_terms: D016475:3T3 Cells; D000818:Animals; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D016348:Osseointegration; D010084:Oxidation-Reduction; D016062:Porosity; D019736:Prostheses and Implants; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D014025:Titanium; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite; D014025:Titanium
references: Angew Chem Int Ed Engl. 2011 Nov 25;50(48):11312-59
references: 22058070
references: Dent Mater. 2005 Nov;21(11):1017-24
references: 15882899
references: J Mater Sci Mater Med. 1997 Apr;8(4):193-200
references: 15348759
references: Nanotechnology. 2008 Jan 16;19(2):025710
references: 21817559
references: Materials (Basel). 2010 Jul 09;3(7):3994-4050
references: 28883319
references: Biomaterials. 2004 Feb;25(3):443-50
references: 14585692
references: Biomaterials. 1998 Sep;19(18):1621-39
references: 9839998
references: J Mater Sci Mater Med. 2008 Dec;19(12):3489-95
references: 18622764
references: J Biomed Mater Res. 1999 May;45(2):100-7
references: 10397963
references: Biomaterials. 2002 Jan;23(1):313-7
references: 11762851
references: Micron. 2013 Jan;44:21-44
references: 22770618
references: Biomaterials. 2004 Jun;25(14):2867-75
references: 14962565
references: J Biomed Mater Res. 1996 Nov;32(3):409-17
references: 8897146
references: J Tissue Eng Regen Med. 2007 Jan-Feb;1(1):25-32
references: 18038389
references: Nanoscale. 2010 Aug;2(8):1358-74
references: 20845524