title: Biocompatibility and characterization of a peptide amphiphile hydrogel for applications in peripheral nerve regeneration.
abstract: Peripheral nerve injury is a debilitating condition for which new bioengineering solutions are needed. Autografting, the gold standard in treatment, involves sacrifice of a healthy nerve and results in loss of sensation or function at the donor site. One alternative solution to autografting is to use a nerve guide conduit designed to physically guide the nerve as it regenerates across the injury gap. Such conduits are effective for short gap injuries, but fail to surpass autografting in long gap injuries. One strategy to enhance regeneration inside conduits in long gap injuries is to fill the guide conduits with a hydrogel to mimic the native extracellular matrix found in peripheral nerves. In this work, a peptide amphiphile (PA)-based hydrogel was optimized for peripheral nerve repair. Hydrogels consisting of the PA C16GSH were compared with a commercially available collagen gel. Schwann cells, a cell type important in the peripheral nerve regenerative cascade, were able to spread, proliferate, and migrate better on C16GSH gels in vitro when compared with cells seeded on collagen gels. Moreover, C16GSH gels were implanted subcutaneously in a murine model and were found to be biocompatible, degrade over time, and support angiogenesis without causing inflammation or a foreign body immune response. Taken together, these results help optimize and instruct the development of a new synthetic hydrogel as a luminal filler for conduit-mediated peripheral nerve repair.
authors: 1 Department of Bioengineering, University of California Berkeley , Berkeley, California.
authors: Katie A
authors: KA
authors: Black
authors: Brian F
authors: BF
authors: Lin
authors: Emily A
authors: EA
authors: Wonder
authors: Seema S
authors: SS
authors: Desai
authors: Eun Ji
authors: EJ
authors: Chung
authors: Bret D
authors: BD
authors: Ulery
authors: Ravi S
authors: RS
authors: Katari
authors: Matthew V
authors: MV
authors: Tirrell
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D005260:Female; D005782:Gels / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects; D008807:Mice, Inbred BALB C; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D010455:Peptides; D013501:Surface-Active Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen
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