title: The zinc finger transcription factor SlZFP2 negatively regulates abscisic acid biosynthesis and fruit ripening in tomato.
abstract: Abscisic acid (ABA) regulates plant development and adaptation to environmental conditions. Although the ABA biosynthesis pathway in plants has been thoroughly elucidated, how ABA biosynthetic genes are regulated at the molecular level during plant development is less well understood. Here, we show that the tomato (Solanum lycopersicum) zinc finger transcription factor SlZFP2 is involved in the regulation of ABA biosynthesis during fruit development. Overexpression of SlZFP2 resulted in multiple phenotypic changes, including more branches, early flowering, delayed fruit ripening, lighter seeds, and faster seed germination, whereas down-regulation of its expression caused problematic fruit set, accelerated ripening, and inhibited seed germination. SlZFP2 represses ABA biosynthesis during fruit development through direct suppression of the ABA biosynthetic genes NOTABILIS, SITIENS, and FLACCA and the aldehyde oxidase SlAO1. We also show that SlZFP2 regulates fruit ripening through transcriptional suppression of the ripening regulator COLORLESS NON-RIPENING. Using bacterial one-hybrid screening and a selected amplification and binding assay, we identified the (A/T)(G/C)TT motif as the core binding sequence of SlZFP2. Furthermore, by RNA sequencing profiling, we found that 193 genes containing the SlZFP2-binding motifs in their promoters were differentially expressed in 2 d post anthesis fruits between the SlZFP2 RNA interference line and its nontransgenic sibling. We propose that SlZFP2 functions as a repressor to fine-tune ABA biosynthesis during fruit development and provides a potentially valuable tool for dissecting the role of ABA in fruit ripening.
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.).
authors: Lin
authors: L
authors: Weng
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.).
authors: Fangfang
authors: F
authors: Zhao
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.).
authors: Rong
authors: R
authors: Li
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.).
authors: Changjie
authors: C
authors: Xu
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.).
authors: Kunsong
authors: K
authors: Chen
authors: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (L.W., F.Z., R.L., H.X.); andFruit Science Institute, Zhejiang University, Hangzhou 310058, China (C.X., K.C.) hanxiao@sibs.ac.cn.
authors: Han
authors: http://orcid.org/0000-0001-5937-6510
authors: H
authors: Xiao
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis*; D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000235:genetics; D005638:Fruit / Q000254:growth & development* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination; D018551:Solanum lycopersicum / Q000254:growth & development* / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction; D012639:Seeds / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D000040:Abscisic Acid
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