title: OxeR1 regulates angiotensin II and cAMP-stimulated steroid production in human H295R adrenocortical cells.
abstract: Hormone-regulated steroidogenesis and StAR protein induction involve the action of lipoxygenated products. The products of 5-lipoxygenase act on inflammation and immunity by stimulation of a membrane receptor called OxeR1. The presence of OxeR1 in other systems has not been described up to date and little is known about its mechanism of action and other functions. In this context, the aim of this study was the identification and characterization of OxeR1 as a mediator of cAMP-dependent and independent pathways. Overexpression of OxeR1 in MA-10 Leydig cells increased cAMP-dependent progesterone production. Angiotensin II and cAMP stimulation of adrenocortical human H295R cells produced an increase in StAR protein induction and steroidogenesis in cells overexpressing OxeR1 as compared to mock-transfected cells. Additionally, activation of OxeR1 caused a time-dependent increase in ERK1/2 phosphorylation. In summary, membrane receptor OxeR1 is involved in StAR protein induction and activation of steroidogenesis triggered by cAMP or angiotensin II, acting, at least in part, through ERK1/2 activation.
authors: INBIOMED - UBA/CONICET, Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina.
authors: Melina
authors: M
authors: Dattilo
authors: INBIOMED - UBA/CONICET, Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina.
authors: Isabel
authors: I
authors: Neuman
authors: INBIOMED - UBA/CONICET, Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina.
authors: Mariana
authors: M
authors: Muñoz
authors: INBIOMED - UBA/CONICET, Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina.
authors: Paula
authors: P
authors: Maloberti
authors: INBIOMED - UBA/CONICET, Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina. Electronic address: fcornejo@fmed.uba.ar.
authors: Fabiana
authors: F
authors: Cornejo Maciel
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010957:Plasmids / Q000378:metabolism; D018055:Receptors, Eicosanoid / Q000378:metabolism*; D013256:Steroids / Q000096:biosynthesis*; D014162:Transfection; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D001095:Arachidonic Acids; C504018:OXER1 protein, human; D010750:Phosphoproteins; D018055:Receptors, Eicosanoid; D013256:Steroids; D000098682:Steroidogenic Acute Regulatory Protein; D000804:Angiotensin II; C080828:5-oxo-6,8,11,14-eicosatetraenoic acid; D000242:Cyclic AMP; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Angiotensin II; ERK1/2; Oxoeicosanoid receptor OxeR1; StAR protein; Steroidogenesis; cAMP