title: Gramella aestuariivivens sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated BG-MY13(T), was isolated from a tidal flat sediment on the South Sea, South Korea. Strain BG-MY13(T) grew optimally at 30-35 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BG-MY13(T) falls within the cluster comprising the type strains of species of the genus Gramella . Strain BG-MY13(T) exhibited 16S rRNA gene sequ4ence similarity values of 96.9-97.8% to the type strains of Gramella echinicola , Gramella gaetbulicola , Gramella portivictoriae and Gramella marina and of 94.6-96.5% to the type strains of other species of the genus Gramella with validly published names. Strain BG-MY13(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0) and iso-C(17 : 0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BG-MY13(T) was 38.9 mol% and DNA-DNA relatedness values with the type strains of G. echinicola , G. gaetbulicola , G. portivictoriae and G. marina were 12-23%. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain BG-MY13(T) is separated from other species of the genus Gramella . On the basis of the data presented, strain BG-MY13(T) is considered to represent a novel species of the genus Gramella , for which the name Gramella aestuariivivens sp. nov. is proposed. The type strain is BG-MY13(T) ( = KCTC 42285(T) = NBRC 110677(T)).
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Ji-Min
authors: JM
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sung-Min
authors: SM
authors: Won
authors: University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.|Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Yong-Taek
authors: YT
authors: Jung
authors: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea.
authors: Kee-Sun
authors: KS
authors: Shin
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6