title: Behavioral and circuit basis of sucrose rejection by Drosophila females in a simple decision-making task.
abstract: Drosophila melanogaster egg-laying site selection offers a genetic model to study a simple form of value-based decision. We have previously shown that Drosophila females consistently reject a sucrose-containing substrate and choose a plain (sucrose-free) substrate for egg laying in our sucrose versus plain decision assay. However, either substrate is accepted when it is the sole option. Here we describe the neural mechanism that underlies females' sucrose rejection in our sucrose versus plain assay. First, we demonstrate that females explored the sucrose substrate frequently before most egg-laying events, suggesting that they actively suppress laying eggs on the sucrose substrate as opposed to avoiding visits to it. Second, we show that activating a specific subset of DA neurons triggered a preference for laying eggs on the sucrose substrate over the plain one, suggesting that activating these DA neurons can increase the value of the sucrose substrate for egg laying. Third, we demonstrate that neither ablating nor inhibiting the mushroom body (MB), a known Drosophila learning and decision center, affected females' egg-laying preferences in our sucrose versus plain assay, suggesting that MB does not mediate this specific decision-making task. We propose that the value of a sucrose substrate- as an egg-laying option-can be adjusted by the activities of a specific DA circuit. Once the sucrose substrate is determined to be the lesser valued option, females execute their decision to reject this inferior substrate not by stopping their visits to it, but by actively suppressing their egg-laying motor program during their visits.
authors: Department of Neurobiology, Duke University Medical School, Durham, North Carolina 27710 and yang@neuro.duke.edu.
authors: Chung-Hui
authors: http://orcid.org/0000-0003-2117-3595
authors: CH
authors: Yang
authors: Department of Neurobiology, Duke University Medical School, Durham, North Carolina 27710 and.
authors: Ruo
authors: R
authors: He
authors: Yang Laboratory, Durham, North Carolina 27705.
authors: Ulrich
authors: U
authors: Stern
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000502:physiology*; D016827:CD8 Antigens / Q000378:metabolism; D003216:Conditioning, Operant / Q000502:physiology; D003657:Decision Making / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D005106:Exploratory Behavior; D005260:Female; D005518:Food Preferences / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D010058:Oviposition / Q000502:physiology; D013395:Sucrose / Q000008:administration & dosage*; D013549:Sweetening Agents / Q000008:administration & dosage*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D013549:Sweetening Agents; D014157:Transcription Factors; D013395:Sucrose; D014446:Tyrosine 3-Monooxygenase
keywords: Drosophila; decision making; dopamine; egg laying; sucrose rejection
references: Neuron. 1996 Jun;16(6):1127-35
references: 8663989
references: Annu Rev Neurosci. 2011;34:333-59
references: 21456961
references: Science. 2014 May 23;344(6186):901-4
references: 24855268
references: Nat Methods. 2009 Jun;6(6):451-7
references: 19412169
references: Development. 1993 Jun;118(2):401-15
references: 8223268
references: Curr Biol. 2013 Dec 16;23(24):2472-80
references: 24316206
references: Cell. 2012 Feb 3;148(3):583-95
references: 22304923
references: Nature. 2012 Dec 20;492(7429):433-7
references: 23103875
references: Neuron. 2012 Mar 8;73(5):941-50
references: 22405204
references: Nature. 2012 Sep 6;489(7414):145-9
references: 22902500
references: Science. 2008 Mar 21;319(5870):1679-83
references: 18356529
references: Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14554-9
references: 12391323
references: J Neurosci. 2008 May 21;28(21):5539-46
references: 18495888
references: Nat Genet. 2004 Mar;36(3):283-7
references: 14981521
references: Nat Neurosci. 2014 Apr;17(4):559-68
references: 24561998
references: J Neurobiol. 2003 Mar;54(4):618-27
references: 12555273
references: Front Neural Circuits. 2009 Jul 01;3:5
references: 19597562
references: PLoS One. 2012;7(5):e37910
references: 22693584
references: Nature. 2012 Aug 23;488(7412):512-6
references: 22810589
references: Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):21153-8
references: 24324162
references: Nature. 2008 Jul 10;454(7201):217-20
references: 18548007
references: Nat Neurosci. 2008 Jan;11(1):95-102
references: 18066060
references: Nature. 2006 May 11;441(7090):223-6
references: 16633341
references: Genetics. 2012 Oct;192(2):521-32
references: 22798487
references: Nature. 1999 Apr 22;398(6729):704-8
references: 10227292
references: Nat Neurosci. 2006 May;9(5):703-9
references: 16582903
references: Science. 1994 Feb 4;263(5147):692-5
references: 8303280
references: J Neurosci. 2007 Jul 18;27(29):7640-7
references: 17634358
references: PLoS One. 2010;5(4):e9954
references: 20376353
references: Neuron. 2007 Nov 8;56(3):503-16
references: 17988633
references: Science. 2007 Jun 29;316(5833):1901-4
references: 17600217
references: Curr Biol. 2012 Nov 20;22(22):2114-23
references: 23022067
references: Neuron. 2013 Dec 4;80(5):1322-36
references: 24314733
references: Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11352-7
references: 19541615
references: Annu Rev Neurosci. 2007;30:31-56
references: 17417936
references: Neuron. 2011 Oct 20;72(2):202-30
references: 22017985
references: J Neurobiol. 2001 May;47(2):81-92
references: 11291099