title: Preparation of on-plate immobilized metal ion affinity chromatography platform via dopamine chemistry for highly selective isolation of phosphopeptides with matrix assisted laser desorption/ionization mass spectrometry analysis.
abstract: In this study, a novel on-plate IMAC technique was developed for highly selective enrichment and isolation of phosphopeptides with high-throughput MALDI-TOF-MS analysis. At first, a MALDI plate was coated with polydopamine (PDA), and then Ti(4+) was immobilized on the PDA-coated plate. The obtained IMAC plate was successfully applied to the highly selective enrichment and isolation of phosphopeptides in protein digests and human serum. Because of no loss of samples, the on-plate IMAC platform exhibits excellent selectivity and sensitivity in the selective enrichment and isolation of phosphopeptides, which provides a potential technique for high selectivity in the detection of low-abundance phosphopeptides in biological samples.
authors: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors: Chenyi
authors: C
authors: Shi
authors: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors: Qinrui
authors: Q
authors: Lin
authors: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors: Chunhui
authors: C
authors: Deng
mesh_terms: D002364:Caseins / Q000737:chemistry; D002846:Chromatography, Affinity; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D010748:Phosphopeptides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000737:chemistry*
chemical_list: D002364:Caseins; D007211:Indoles; D010748:Phosphopeptides; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine; D014025:Titanium
keywords: Dopamine chemistry; Mass spectrometry analysis; On-plate IMAC platform; Selective enrichment and isolation of phosphopeptides