title: [Grape seed proanthocyanidins extract inhibits pancreatic cancer cell growth through down-regulation of miR-27a expression].
abstract: OBJECTIVE
  To explore the eff ect of grape seed proanthocyanidins extract (GSPE) on the growth of pancreatic cancer cells and the underlying mechanisms.
  
  
  METHODS
  The pancreatic cancer AsPC-1 cells were cultured in vitro. The effects of GSPE on cell proliferation, apoptosis and migration were analyzed by MTT, Annexin V-FITC/PI and Transwell migration assay, respectively. The expression of miR-27a and FOXO1 in AsPC-1 cells was determined by real-time RT-PCR and Western blot, respectively. The miR-27a inhibitors were applied to verify the role of miR-27a in mediation of GSPE effects.
  
  
  RESULTS
  GSPE inhibited cell growth in a dose-dependent manner. This inhibitory effect was significant when the dosage of GSPE was more than 50 μg/mL (P<0.05 vs control). GSPE also could induce apoptosis and inhibit cell migration. MiR-27a expression was notably down-regulated when the dosage of GSPE was 75 μg/mL (P<0.01 vs control). Compared with the control group, cell proliferation inhibition was significantly increased in the miR-27a inhibitor group, the GSPE group and the miR-27a inhibitor plus GSPE group (P<0.01), while cell migration was significantly decreased (P<0.01). Compared with the GSPE or the miR-27a inhibitor group, the growth and migration inhibitory effects in the miR-27a inhibitor plus GSPE group were more obviously (P<0.01). Both GSPE and miR-27a inhibitor alone could up-regulate FOXO1 expression. But these effects were more apparent when they are applied in combination.
  
  
  CONCLUSIONS
  GSPE inhibites AsPC-1 cells' growth and migration partly through down-regulation of miR-27a expression.
authors: Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu Anhui 233030, China.
authors: Jia
authors: J
authors: Ma
authors: Binbin
authors: B
authors: Fang
authors: Fanpeng
authors: F
authors: Zeng
authors: Haijie
authors: H
authors: Pang
authors: Cong
authors: C
authors: Ma
authors: Jun
authors: J
authors: Xia
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D056604:Grape Seed Extract / Q000494:pharmacology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D044945:Proanthocyanidins