title: Enhancement of osteogenesis post-splenectomy does not attenuate bone loss in ovariectomized rats.
abstract: The roles of different immune cell populations and cytokines in bone metabolism have been extensively investigated. However, the influence of whole immune organ removal on osteopathology remains unknown. In the current study, we investigated the effects of splenectomy on bone metabolism and microarchitecture in rats with or without concurrent ovariectomy. Ovariectomized (OVX) rats were used as osteoporosis model. Sixty 12-week-old female rats were randomized into 4 groups (n = 15): sham, splenectomized (SP), ovariectomized, as well as ovariectomized and splenectomized (OVX + SP). Bone microarchitecture was assessed by micro CT analysis at 4 week and 12 week post-operation, respectively. Bone pathology and metabolism were evaluated via immunohistochemical staining. The serum levels of alkaline phosphatase (ALP), tumor necrosis factor-alpha (TNF-α), tartrate-resistant acid phosphatase 5b (Tracp5b), and C-terminal telopeptide (CTx) were analyzed at 4 and 12 weeks post-operation. Removal of the spleen led to alterations in the homeostasis of bone metabolism and increased bone formation in rats. In this study, our findings indicate that the spleen is involved in skeletal metabolism.
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.|Department of Orthopedics & Microsurgery, Guangdong No. 2 Provincial People's Hospital, Guangzhou, 510317, China.
authors: Dawei
authors: D
authors: Sun
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.|Department of Orthopedics, the Second Affiliated Hospital of Luohe Medical College, Luohe, 462300, China.
authors: Xiaochen
authors: X
authors: Zheng
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
authors: Yuhui
authors: Y
authors: Chen
authors: Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou, 510515, China.
authors: Chunhong
authors: C
authors: Jia
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.|Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou, 510515, China.
authors: Song
authors: S
authors: Xu
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
authors: Chuangxin
authors: C
authors: Lin
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
authors: Pei
authors: P
authors: Zhang
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
authors: Zhongmin
authors: Z
authors: Zhang
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
authors: Daozhang
authors: D
authors: Cai
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.
authors: Dadi
authors: D
authors: Jin
authors: Department of Rehabilitation Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.
authors: Baiyu
authors: B
authors: Zhang
authors: Department of Orthopedics, Academy of Orthopedics, Guangdong Province, The Third Affiliated Hospital, Southern Medical University, Guangzhou, 510665, China.|Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou, 510515, China.
authors: Xiaochun
authors: X
authors: Bai
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001835:Body Weight; D001851:Bone Diseases, Metabolic / Q000503:physiopathology*; D001842:Bone and Bones / Q000473:pathology; D024042:Collagen Type I / Q000097:blood; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000097:blood; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis; D010024:Osteoporosis / Q000503:physiopathology*; D010052:Ovariectomy; D010455:Peptides / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013156:Splenectomy; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055114:X-Ray Microtomography
chemical_list: D024042:Collagen Type I; D007527:Isoenzymes; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C052929:collagen type I trimeric cross-linked peptide; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: osteoclastogenesis; osteogenesis; osteoporosis; ovariectomy; splenectomy