title: Induction of colorectal cancer in mice and histomorphometric evaluation of tumors.
abstract: Colorectal cancer (CRC) originates from the epithelial cells lining the colon or rectum of the gastrointestinal tract and represents the third most common form of cancer worldwide. CRC is frequently associated with Colitis Ulcerosa or Crohn's Disease demonstrating the tumor-promoting role of inflammation. Colorectal tumor cells establish heterotypic interactions with inflammatory cells and cancer-associated fibroblasts in the tumor stroma that support tumor angiogenesis and are essential for tumor progression. Therefore, establishment of suitable mouse models mimicking the inflammatory etiology of CRC is important. Here we describe methods to induce CRC in mice, to quantify tumor parameters (multiplicity, tumor load, mean tumor size), and to analyze the cellular composition of the CRC tumor stroma.
authors: Institute of Cancer Research (ICR) & Comprehensive Cancer Center (CCC), Medical University Vienna, Borschkegasse 8a, 1090, Vienna, Austria.
authors: Ilija
authors: I
authors: Crncec
authors: Paulina
authors: P
authors: Pathria
authors: Jasmin
authors: J
authors: Svinka
authors: Robert
authors: R
authors: Eferl
mesh_terms: D000818:Animals; D001397:Azoxymethane / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000378:metabolism; D012984:Software; D013194:Staining and Labeling; D013431:Sulfates / Q000494:pharmacology; D047368:Tumor Burden
chemical_list: D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C470944:S100a4 protein, mouse; D013431:Sulfates; D017479:Receptors, Platelet-Derived Growth Factor; D001397:Azoxymethane; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin