title: Comparison of immune responses to intranasal and intrapulmonary vaccinations with the attenuated Mycoplasma hyopneumoniae 168 strain in pigs.
abstract: The aim of this study was to evaluate the immune responses to intranasal and intrapulmonary vaccinations with the attenuated Mycoplasma hyopneumoniae (Mhp) 168 strain in the local respiratory tract in pigs. Twenty-four pigs were randomly divided into 4 groups: an intranasal immunization group, an intrapulmonary immunization group, an intramuscular immunization group and a control group. The levels of local respiratory tract cellular and humoral immune responses were investigated. The levels of interleukin (IL)-6 in the early stage of immunization (P<0.01), local specific secretory IgA (sIgA) in nasal swab samples (P<0.01); and IgA- and IgG-secreting cells in the nasal mucosa and trachea were higher after intranasal vaccination (P<0.01) than in the control group. Interestingly, intrapulmonary immunization induced much stronger immune responses than intranasal immunization. Intrapulmonary immunization also significantly increased the secretion of IL-6 and local specific sIgA and the numbers of IgA- and IgG-secreting cells. The levels of IL-10 and interferon-γ in the nasal swab samples and the numbers of CD4(+) and CD8(+) T lymphocytes in the lung and hilar lymph nodes were significantly increased by intrapulmonary immunization compared with those in the control group (P<0.01). These data suggest that intrapulmonary immunization with attenuated Mhp is effective in evoking local cellular and humoral immune responses in the respiratory tract. Intrapulmonary immunization with Mhp may be a promising route for defense against Mhp in pigs.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors: Pengcheng
authors: P
authors: Li
authors: Yunfeng
authors: Y
authors: Li
authors: Guoqing
authors: G
authors: Shao
authors: Qinghua
authors: Q
authors: Yu
authors: Qian
authors: Q
authors: Yang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004333:Drug Administration Routes; D009175:Mycoplasma Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D045705:Mycoplasma hyopneumoniae / Q000145:classification*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014613:Vaccines, Attenuated
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D014613:Vaccines, Attenuated
references: Am J Vet Res. 2000 Nov;61(11):1384-9
references: 11108184
references: Vaccine. 2007 Apr 30;25(17):3254-62
references: 17303292
references: Vet Immunol Immunopathol. 2002 Sep 10;87(3-4):161-8
references: 12072230
references: Nat Rev Immunol. 2003 Jan;3(1):79-84
references: 12511878
references: Crit Rev Ther Drug Carrier Syst. 2004;21(1):21-66
references: 15099184
references: Reg Immunol. 1990 Jan-Feb;3(1):62-71
references: 2223558
references: Vet Microbiol. 1992 Mar;30(4):329-41
references: 1533978
references: Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11187-91
references: 7972032
references: Microbiol Immunol. 1995;39(5):351-9
references: 7565176
references: Microb Pathog. 1999 Jun;26(6):325-31
references: 10343061
references: Res Vet Sci. 1999 Aug;67(1):47-52
references: 10425240
references: Expert Opin Drug Deliv. 2006 Jan;3(1):87-95
references: 16370942
references: Vaccine. 2012 Mar 9;30(12):2153-8
references: 22266290
references: Biotechnol Lett. 2008 Feb;30(2):229-33
references: 17928958
references: Mucosal Immunol. 2008 Nov;1(6):489-96
references: 19079216
references: Vet Immunol Immunopathol. 2009 May 15;129(1-2):76-81
references: 19157567
references: J Pediatr. 2010 Feb;156(2 Suppl):S8-15
references: 20105666
references: Vet Microbiol. 2010 Jul 14;143(2-4):410-6
references: 20053508
references: Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):152-8
references: 21176971
references: Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):312-6
references: 21272944
references: Vaccine. 2011 May 23;29(23):4067-76
references: 21419164
references: J Infect Dis. 2011 Nov;204 Suppl 3:S1032-42
references: 21987739
references: Clin Vaccine Immunol. 2011 Nov;18(11):1996-8
references: 21918121
references: Rev Med Virol. 2001 Mar-Apr;11(2):117-33
references: 11262530