title: Annotation of proteins of unknown function: initial enzyme results.
abstract: Working with a combination of ProMOL (a plugin for PyMOL that searches a library of enzymatic motifs for local structural homologs), BLAST and Pfam (servers that identify global sequence homologs), and Dali (a server that identifies global structural homologs), we have begun the process of assigning functional annotations to the approximately 3,500 structures in the Protein Data Bank that are currently classified as having "unknown function". Using a limited template library of 388 motifs, over 500 promising in silico matches have been identified by ProMOL, among which 65 exceptionally good matches have been identified. The characteristics of the exceptionally good matches are discussed.
authors: College of Science, RIT, Rochester, NY, USA.
authors: Talia
authors: T
authors: McKay
authors: Kaitlin
authors: K
authors: Hart
authors: Alison
authors: A
authors: Horn
authors: Haeja
authors: H
authors: Kessler
authors: Greg
authors: G
authors: Dodge
authors: Keti
authors: K
authors: Bardhi
authors: Kostandina
authors: K
authors: Bardhi
authors: Jeffrey L
authors: JL
authors: Mills
authors: Herbert J
authors: HJ
authors: Bernstein
authors: Paul A
authors: PA
authors: Craig
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D030562:Databases, Protein; D008958:Models, Molecular; D058977:Molecular Sequence Annotation / Q000379:methods*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D017386:Sequence Homology, Amino Acid; D012984:Software
chemical_list: D011506:Proteins
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