title: Measurement of cell traction forces with ImageJ.
abstract: The quantification of cell traction forces requires three key steps: cell plating on a deformable substrate, measurement of substrate deformation, and the numerical estimation of the corresponding cell traction forces. The computing steps to measure gel deformation and estimate the force field have somehow limited the adoption of this method in cell biology labs. Here we propose a set of ImageJ plug-ins so that every lab equipped with a fluorescent microscope can measure cell traction forces.
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Jean-Louis
authors: JL
authors: Martiel
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Aldo
authors: A
authors: Leal
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Laetitia
authors: L
authors: Kurzawa
authors: Laboratoire Interdisciplinaire de Physique, UMR 5588, CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Martial
authors: M
authors: Balland
authors: Laboratoire Interdisciplinaire de Physique, UMR 5588, CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Irene
authors: I
authors: Wang
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Timothée
authors: T
authors: Vignaud
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France.
authors: Qingzong
authors: Q
authors: Tseng
authors: CytoMorpho Lab, Institut de Recherche en Technologie et Science pour le Vivant, LPCV/UMR5168, CEA/INRA/CNRS/Univ. Grenoble-Alpes, Grenoble, France; CytoMorpho Lab, Hopital Saint Louis, Institut Universitaire d'Hematologie, U1160, INSERM/AP-HP/Université Paris Diderot, Paris, France.
authors: Manuel
authors: M
authors: Théry
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D005782:Gels; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D012984:Software; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D016326:Extracellular Matrix Proteins; D005782:Gels; D000099166:Polyacrylamides
keywords: Cell mechanics; Contractility; Hydrogel; ImageJ plug-in; Polyacrylamide; Traction force microscopy