title: Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells.
abstract: In this study, we used a microchamber to observe and analyze the gliding phenomenon of Navicula pavillardii diatom cells at different temperatures. The temperature of the culture medium was varied from 17.0 to 30.0 °C to examine the effect of temperature on diatom movement. Movement of each cell at different temperatures was monitored by use of an inverted optical microscope and continuously recorded as video data, from which the velocities of each cell were calculated, by using dedicated software to perform two-dimensional trajectory analysis. The velocities of the same cell at different temperatures were thereby successfully compared. The results showed that the change in cell velocity was insignificant when the temperature was increased from 17.0 to 25.0 °C. When the temperature was increased from 17.0 to 27.5 °C, non-uniformly disrupted cell movement was observed. When the temperature was further increased to 30.0 °C, cell movement was clearly inhibited. By use of single-cell analysis, the effects of the temperature increases on diatom movement were successfully evaluated. Finally, we characterized the experimental data by performing t tests to evaluate the effects of variations of the movement of individual cells on the data analysis.
authors: Department of Physics, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 162-8601, Japan, meicun2006@163.com.
authors: Kazuo
authors: K
authors: Umemura
authors: Toru
authors: T
authors: Miyabayashi
authors: Hisao
authors: H
authors: Taira
authors: Akira
authors: A
authors: Suzuki
authors: Yoshikazu
authors: Y
authors: Kumashiro
authors: Teruo
authors: T
authors: Okano
authors: Shigeki
authors: S
authors: Mayama
mesh_terms: D000465:Algorithms; D017377:Diatoms / Q000502:physiology*; D006358:Hot Temperature; D044085:Microfluidics / Q000379:methods; D009038:Motion; D059010:Single-Cell Analysis / Q000379:methods
references: J Microbiol Methods. 2013 Mar;92(3):349-54
references: 23337812
references: Plant Physiol. 1980 Jan;65(1):129-31
references: 16661126
references: Cell Motil Cytoskeleton. 1999;44(1):23-33
references: 10470016
references: J Microbiol Methods. 2011 Dec;87(3):316-9
references: 21963449
references: Proc Natl Acad Sci U S A. 1970 Sep;67(1):338-44
references: 16591861
references: Bioelectromagnetics. 1999;20(2):94-100
references: 10029135
references: J Gen Microbiol. 1958 Apr;18(2):427-32
references: 13525659
references: Ann Rev Mar Sci. 2010;2:333-65
references: 21141668
references: Eur Biophys J. 2012 Jun;41(6):545-50
references: 22484858
references: Nature. 2009 May 14;459(7244):185-92
references: 19444204
references: Exp Cell Res. 1972 Oct;74(2):552-8
references: 5080793