title: Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase.
abstract: Effect of female sex hormones on the production/release of adipocyte-derived cytokines has been debatable. Furthermore, whether the cellular signaling triggered by these hormones involve Rho-kinase has not been investigated yet. Therefore, in this study, effects of 17β-estradiol and progesterone as well as the Rho-kinase inhibitor, Y-27632 on the level of adipokines such as resistin, adiponectin, leptin, TNF-α and IL-6 were investigated in 3T3-L1-derived adipocytes. Differentiation was induced in the post-confluent preadipocytes by the standard differentiation medium (Dulbecco's modified Eagle's medium with 10% fetal bovine serum together with the mixture of isobutylmethylxanthine, dexamethasone and insulin) in the presence of 17β-estradiol (10(-8)-10(-7)M), progesterone (10(-6)-10(-5)M), the Rho-kinase inhibitor, Y-27632 (10(-5)M) and their combination for 8days. Measurements of the adipokines were performed in the culturing medium by ELISA kits using specific monoclonal antibodies. 17β-estradiol elevated resistin but decreased adiponectin and IL-6 levels; however, it did not alter the concentration of leptin and TNF-α. Y-27632 pretreatment inhibited the rise of resistin and the fall of adiponectin by 17β-estradiol without any effects by its own. Progesterone did not change resistin, leptin and TNF-α level; however, it elevated adiponectin and decreased IL-6 production. Neither 17β-estradiol nor Y-27632 was able to antagonize the increase of adiponectin and the reduction of IL-6 levels by progesterone. While Y-27632 alone lowered IL-6 level, it increased leptin and TNF-α concentration without altering resistin and adiponectin. In conclusion, 17β-estradiol could modify adipokine production in 3T3-L1 adipocytes with the actions some of which involve Rho-kinase mediation.
authors: Department of Pharmacology Medical Faculty, Mersin University Campus Ciftlikkoy, 33343 Mersin, Turkey.
authors: Mehtap
authors: M
authors: Pektaş
authors: Department of Pharmacology Medical Faculty Sutcu Imam University, Kahramanmaras, Turkey.
authors: Akif Hakan
authors: AH
authors: Kurt
authors: Department of Pharmacology Medical Faculty, Mersin University Campus Ciftlikkoy, 33343 Mersin, Turkey. Electronic address: unisfarma@yahoo.com.
authors: İsmail
authors: İ
authors: Ün
authors: Department of Pharmacology Medical Faculty, Mersin University Campus Ciftlikkoy, 33343 Mersin, Turkey.
authors: Rukiye Nalan
authors: RN
authors: Tiftik
authors: Department of Pharmacology Medical Faculty, Mersin University Campus Ciftlikkoy, 33343 Mersin, Turkey.
authors: Kansu
authors: K
authors: Büyükafşar
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis; D054392:Adipokines / Q000096:biosynthesis* / Q000502:physiology*; D052242:Adiponectin / Q000096:biosynthesis; D000577:Amides / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D020738:Leptin / Q000096:biosynthesis; D051379:Mice; D011374:Progesterone / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology; D052243:Resistin / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D054392:Adipokines; D052242:Adiponectin; D000577:Amides; D016207:Cytokines; D015850:Interleukin-6; D020738:Leptin; D011725:Pyridines; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; C108830:Y 27632; D011374:Progesterone; D004958:Estradiol; D054460:rho-Associated Kinases
keywords: 17β-estradiol; Adipocyte; Adipokines; Progesterone; Rho-kinase