title: Overexpression of p28GANK accelerates the metastasis of oesophageal squamous cell carcinoma.
abstract: The present study aimed to evaluate the effects of p28GANK expression on the metastasis of oesophageal squamous cell carcinoma (ESCC) tissues and to investigate its roles in the metastasis of highly invasive and non‑invasive ESCC cell lines. Quantitative polymerase chain reaction (qPCR) and immunohistochemical analyses were performed to assess p28GANK mRNA and protein expression in ESCC tissues and to analyse its significance in ESCC metastasis. qPCR and western blot analyses were used to detect p28GANK mRNA and protein expression in highly invasive and non‑invasive cell lines. Subsequently, lentivirus‑mediated p28GANK short interfering RNA (siRNA) was transfected into highly invasive ESCC cells, and Transwell assays were performed to analyse the effects of p28GANK knockdown on their migration and invasion. The mean expression levels of p28GANK mRNA in the ESCC tissues of patients with metastasis were signiﬁcantly higher than those in the ESCC specimens from patients without metastasis. p28GANK expression in ESCC tissues was correlated with T‑stage, lymph node metastasis and lymphatic invasion. The mRNA and protein expression levels of p28GANK were significantly higher in highly invasive cell lines compared with those of matched, non‑invasive cell lines. Lentivirus‑mediated siRNA knockdown of p28GANK markedly decreased p28GANK expression in EC109‑P and EC9706‑P cells and supressed the metastasis of ESCC cells in vitro. In conclusion, p28GANK expression was increased in metastatic ESCC tissues and cells, and p28GANK knockdown decreased the metastatic ability of ESCC cells. These results suggested that p28GANK may be a potential therapeutic marker for ESCC metastasis.
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Shanhong
authors: S
authors: Tang
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Jianping
authors: J
authors: Qin
authors: Department of General Surgery, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Weihui
authors: W
authors: Liu
authors: Department of Thoracic Surgery, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Xiushan
authors: X
authors: Zheng
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Xiaoling
authors: X
authors: Wu
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Hui
authors: H
authors: Xu
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Lijuan
authors: L
authors: Qiao
authors: Prevention and Control Centre of Disease of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Quanshui
authors: Q
authors: Fan
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Weizheng
authors: W
authors: Zeng
authors: Department of Digestion, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, P.R. China.
authors: Mingde
authors: M
authors: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference
chemical_list: C403591:PSMD10 protein, human; D011518:Proto-Oncogene Proteins; D046988:Proteasome Endopeptidase Complex