title: Effect of the crude extract of Eugenia uniflora in morphogenesis and secretion of hydrolytic enzymes in Candida albicans from the oral cavity of kidney transplant recipients.
abstract: BACKGROUND
  Candida albicans is a diploid yeast that in some circumstances may cause oral or oropharyngeal infections. Yeasts virulence factors contribute for both the maintenance of colonizing strains in addition to damage and cause tissue invasion, thus the establishment of infection occurs. The limited arsenal of antifungal drugs for the treatment of candidiasis turn the investigation of natural products mandatory for the discovery of new targets for antifungal drug development. Therefore, tropical countries emerge as important providers of natural products with potential antimicrobial activity. This study aimed to investigate morphogenesis and secretion of hydrolytic enzymes (phospholipase and proteinase) in the presence of the CE of Eugenia uniflora.
  
  
  METHODS
  The isolates were tested for their ability to form hyphae in both solid and liquid media under three different conditions: YPD + 20% FBS, Spider medium and GlcNac and the ability to secrete phospholipase and proteinase in the presence of 2000 μg/mL of E. uniflora.
  
  
  RESULTS
  The CE of E. uniflora inhibited hypha formation in both liquid and solid media tested. It also impaired hydrolytic enzymes production.
  
  
  CONCLUSIONS
  This was the first study to describe the interaction of a natural product with the full expression of three different factors in C. albicans. E. uniflora may be an alternative therapeutic for oral candidiasis in the future.
authors: Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. wrplinio@hotmail.com.
authors: Walicyranison Plinio
authors: WP
authors: Silva-Rocha
authors: Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. vitorluiz_06@yahoo.com.br.
authors: Vitor Luiz
authors: VL
authors: de Brito Lemos
authors: Departamento de Farmácia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. magda_nani@hotmail.com.
authors: Magda Rhayanny Assunção
authors: MR
authors: Ferreira
authors: Departamento de Farmácia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. phtech@uol.com.br.
authors: Luiz Alberto Lira
authors: LA
authors: Soares
authors: Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. terezinha@email.com.
authors: Terezinha Inês Estivalet
authors: TI
authors: Svidzisnki
authors: Departamento de Infectologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. evepipolo@gmail.com.
authors: Eveline Pipolo
authors: EP
authors: Milan
authors: Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. guilherme.chaves@ufrnet.br.
authors: Guilherme Maranhão
authors: GM
authors: Chaves
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D002180:Candidiasis, Oral / Q000188:drug therapy* / Q000209:etiology / Q000382:microbiology; D045424:Complex Mixtures; D006801:Humans; D006868:Hydrolysis; D025301:Hyphae / Q000187:drug effects; D007668:Kidney / Q000378:metabolism / Q000601:surgery; D016030:Kidney Transplantation / Q000009:adverse effects; D009024:Morphogenesis / Q000187:drug effects; D010447:Peptide Hydrolases / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D027842:Syzygium; D066027:Transplant Recipients; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D045424:Complex Mixtures; D010936:Plant Extracts; D037521:Virulence Factors; D010740:Phospholipases; D010447:Peptide Hydrolases
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