title: Perturbation of maize phenylpropanoid metabolism by an AvrE family type III effector from Pantoea stewartii.
abstract: AvrE family type III effector proteins share the ability to suppress host defenses, induce disease-associated cell death, and promote bacterial growth. However, despite widespread contributions to numerous bacterial diseases in agriculturally important plants, the mode of action of these effectors remains largely unknown. WtsE is an AvrE family member required for the ability of Pantoea stewartii ssp. stewartii (Pnss) to proliferate efficiently and cause wilt and leaf blight symptoms in maize (Zea mays) plants. Notably, when WtsE is delivered by a heterologous system into the leaf cells of susceptible maize seedlings, it alone produces water-soaked disease symptoms reminiscent of those produced by Pnss. Thus, WtsE is a pathogenicity and virulence factor in maize, and an Escherichia coli heterologous delivery system can be used to study the activity of WtsE in isolation from other factors produced by Pnss. Transcriptional profiling of maize revealed the effects of WtsE, including induction of genes involved in secondary metabolism and suppression of genes involved in photosynthesis. Targeted metabolite quantification revealed that WtsE perturbs maize metabolism, including the induction of coumaroyl tyramine. The ability of mutant WtsE derivatives to elicit transcriptional and metabolic changes in susceptible maize seedlings correlated with their ability to promote disease. Furthermore, chemical inhibitors that block metabolic flux into the phenylpropanoid pathways targeted by WtsE also disrupted the pathogenicity and virulence activity of WtsE. While numerous metabolites produced downstream of the shikimate pathway are known to promote plant defense, our results indicate that misregulated induction of phenylpropanoid metabolism also can be used to promote pathogen virulence.
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Jo Ann E
authors: JE
authors: Asselin
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Jinshan
authors: J
authors: Lin
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Alvaro L
authors: AL
authors: Perez-Quintero
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Irene
authors: I
authors: Gentzel
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Doris
authors: D
authors: Majerczak
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Stephen O
authors: SO
authors: Opiyo
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Wanying
authors: W
authors: Zhao
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Seung-Mann
authors: SM
authors: Paek
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Min Gab
authors: MG
authors: Kim
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: David L
authors: DL
authors: Coplin
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.).
authors: Joshua J
authors: JJ
authors: Blakeslee
authors: Department of Horticulture and Crop Science (J.E.A., J.L., A.L.P.-Q., Do.M., W.Z., J.J.B., Da.M.), Molecular and Cellular Imaging Center-Columbus, Ohio Agricultural Research and Development Center (J.L., S.O.O., J.J.B.), Translational Plant Sciences Graduate Program (I.G.), Center for Applied Plant Sciences (I.G., Da.M.), Department of Plant Pathology (D.L.C.), and Department of Molecular Genetics (Da.M.), Ohio State University, Columbus, Ohio 43210; andCollege of Pharmacy, Research Institute of Pharmaceutical Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-751, Republic of Korea (S.-M.P., M.G.K.) mackey.86@osu.edu.
authors: David
authors: D
authors: Mackey
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D058947:Bacterial Secretion Systems / Q000187:drug effects; D001681:Biological Assay; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D018745:Genome, Plant; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D020636:Pantoea / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D020005:Propanols / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D012765:Shikimic Acid / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014439:Tyramine; D014774:Virulence / Q000187:drug effects; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D020005:Propanols; C439395:1-phenylpropanol; D012765:Shikimic Acid; D010650:Phenylalanine Ammonia-Lyase; D014439:Tyramine
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