title: [Differential expressions of serum cytokines in cognitive dysfunction patients after colorectal surgery].
abstract: OBJECTIVE
  To evaluate the serum levels of cytokines in postoperative cognitive dysfunction (POCD) patients having received colorectal surgery.
  
  
  METHODS
  Eighty patients who had received colorectal surgery were enrolled in the study based on the defined inclusion and exclusion criteria. Cognitive function was assessed on day 1 before operation and day 7 after operation using a battery of neuropsychological tests. Serum levels of 120 cytokines were measured at two time points (day 1 before surgery and 4 hours after surgery) using high-throughput cytokine screening. Serum interleukin 6 (IL-6), IL-11, IL-17, Fas, methyl-accepting chemotaxis protein 3 (MCP3), myeloid progenitor inhibitory factor-1 (MPIF-1), macrophage inflammatory protein 3 alpha (MIP-3α), brain-derived neurotrophic factor (BDNF) were determined by ELISA.
  
  
  RESULTS
  Seventy-seven patients completed both neurocognitive tests and blood samples collection. Nineteen cases were diagnosed with POCD at 7 days after surgery. The age, ASA classification and diabetes incidence of POCD patients were significantly higher and the education level was lower than those in patients without POCD. Serum IL-17, Fas, MIP-3α levels were higher and leptin was lower when compared with non-POCD patients at 1 day before surgery. Serum IL-6, IL-11, IL-17, MCP3, MPIF-1, MIP-3α levels were higher and BDNF was lower when compared with non-POCD group at 4 hours after surgery. In POCD group, serum IL-6, IL-17, MCP3 and MPIF-1 levels at 4 hours postoperatively were higher when compared with ones at 1 day preoperatively. In non-POCD group, serum IL-6 and IL-17 at 4 hours postoperatively were higher while Fas and BDNF were lower when compared with ones at 1 day preoperatively.
  
  
  CONCLUSIONS
  Serum cytokines IL-6, IL-11, IL-17, Fas, MPIF-1, MIP-3α, MCP3, leptin, BDNF were found to display differential expressions in POCD patients after colorectal surgery.
authors: Department of Molecular Cell Biology and Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166; Department of Anesthesiology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029; Department of Anesthesiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors: Yuan
authors: Y
authors: Zhang
authors: Department of Anesthesiology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Yanning
authors: Y
authors: Qian
authors: Department of Anesthesiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors: Yanna
authors: Y
authors: Si
authors: Department of Anesthesiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors: Hongguang
authors: H
authors: Bao
authors: Department of Molecular Cell Biology and Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors: Jianwei
authors: J
authors: Zhou
mesh_terms: D000368:Aged; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D054418:Chemokine CCL20 / Q000097:blood; D003072:Cognition Disorders / Q000097:blood* / Q000209:etiology; D003108:Colonic Diseases / Q000601:surgery*; D003107:Colorectal Surgery / Q000009:adverse effects*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D017370:Interleukin-11 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D011183:Postoperative Complications / Q000097:blood* / Q000209:etiology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C108355:CCL20 protein, human; D054418:Chemokine CCL20; D016207:Cytokines; D017370:Interleukin-11; D020381:Interleukin-17; D015850:Interleukin-6