title: Diversity in the Strength and Structure of Unruptured Cerebral Aneurysms.
abstract: Intracranial aneurysms are pathological enlargements of brain arteries that are believed to arise from progressive wall degeneration and remodeling. Earlier work using classical histological approaches identified variability in cerebral aneurysm mural content, ranging from layered walls with intact endothelium and aligned smooth muscle cells, to thin, hypocellular walls. Here, we take advantage of recent advances in multiphoton microscopy, to provide novel results for collagen fiber architecture in 15 human aneurysm domes without staining or fixation as well as in 12 control cerebral arteries. For all aneurysm samples, the elastic lamina was absent and the abluminal collagen fibers had similar diameters to control arteries. In contrast, the collagen fibers on the luminal side showed great variability in both diameter and architecture ranging from dense fiber layers to sparse fiber constructs suggestive of ineffective remodeling efforts. The mechanical integrity of eight aneurysm samples was assessed using uniaxial experiments, revealing two sub-classes (i) vulnerable unruptured aneurysms (low failure stress and failure pressure), and (ii) strong unruptured aneurysms (high failure stress and failure pressure). These results suggest a need to refine the end-point of risk assessment studies that currently do not distinguish risk levels among unruptured aneurysms. We propose that a measure of wall integrity that identifies this vulnerable wall subpopulation will be useful for interpreting future biological and structural data.
authors: Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA, USA, rbertson@pitt.edu.
authors: Anne M
authors: AM
authors: Robertson
authors: Xinjie
authors: X
authors: Duan
authors: Khaled M
authors: KM
authors: Aziz
authors: Michael R
authors: MR
authors: Hill
authors: Simon C
authors: SC
authors: Watkins
authors: Juan R
authors: JR
authors: Cebral
mesh_terms: D000328:Adult; D000368:Aged; D001696:Biomechanical Phenomena; D002536:Cerebral Arteries / Q000378:metabolism / Q000503:physiopathology*; D003094:Collagen / Q000378:metabolism; D005260:Female; D006801:Humans; D002532:Intracranial Aneurysm / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton; D008875:Middle Aged; D013314:Stress, Mechanical
chemical_list: D003094:Collagen
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