title: Comparative effects of mesenchymal stem cell therapy in distinct stages of chronic renal failure.
abstract: BACKGROUND
  The therapeutic potential of adult stem cells in the treatment of chronic diseases is becoming increasingly evident. In the present study, we sought to assess whether treatment with mesenchymal stem cells (MSCs) efficiently retards progression of chronic renal failure (CRF) when administered to experimental models of less severe CRF.
  
  
  METHODS
  We used two renal mass reduction models to simulate different stages of CRF (5/6 or 2/3 mass renal reduction). Renal functional parameters measured were serum creatinine (SCr), creatinine clearance (CCr), rate of decline in CCr (RCCr), and 24-h proteinuria (PT24h). We also evaluated renal morphology by histology and immunohistochemistry. MSCs were obtained from bone marrow aspirates and injected into the renal parenchyma of the remnant kidneys of both groups of rats with CRF (MSC5/6 or MSC2/3).
  
  
  RESULTS
  Animals from groups MSC5/6 and CRF2/3 seemed to benefit from MSC therapy because they showed significantly reduction in SCr and PT24h, increase in CCr and slowed the RCCr after 90 days. Treatment reduced glomerulosclerosis but significant improvement did occur in the tubulointerstitial compartment with much less fibrosis and atrophy. MSC therapy reduced inflammation by decreasing macrophage accumulation proliferative activity (PCNA-positive cells) and fibrosis (α-SM-actin). Comparisons of renal functional and morphological parameters responses between the two groups showed that rats MSC2/3 were more responsive to MSC therapy than MSC5/6.
  
  
  CONCLUSIONS
  This study showed that MSC therapy is efficient to retard CRF progression and might be more effective when administered during less severe stages of CRF.
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.
authors: Heloisa Cristina
authors: HC
authors: Caldas
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.
authors: Thaís Amarante Peres
authors: TA
authors: de Paula Couto
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.
authors: Ida Maria Maximina
authors: IM
authors: Fernandes
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.
authors: Maria Alice Sperto Ferreira
authors: MA
authors: Baptista
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.|Genetics and Molecular Biology Research Unit Laboratory-UPGEM, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Sao Jose do Rio Preto, SP, Brazil.
authors: Rosa Sayoko
authors: RS
authors: Kawasaki-Oyama
authors: Genetics and Molecular Biology Research Unit Laboratory-UPGEM, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Sao Jose do Rio Preto, SP, Brazil.
authors: Eny Maria
authors: EM
authors: Goloni-Bertollo
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil.
authors: Domingo Marcolino
authors: DM
authors: Braile
authors: Laboratory of Immunology and Experimental Transplantation-LITEX, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Av Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, SP, 15090-000, Brazil. mabbud@terra.com.br.|Genetics and Molecular Biology Research Unit Laboratory-UPGEM, Department of Medicine/Nephrology, Medical School, FAMERP-HB/FUNFARME, Sao Jose do Rio Preto, SP, Brazil. mabbud@terra.com.br.|Instituto de Urologia e Nefrologia, Sao Jose do Rio Preto, SP, Brazil. mabbud@terra.com.br.
authors: Mario
authors: M
authors: Abbud-Filho
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation; D003404:Creatinine / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005355:Fibrosis / Q000473:pathology; D005923:Glomerulosclerosis, Focal Segmental / Q000473:pathology / Q000628:therapy; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000473:pathology / Q000628:therapy*; D007677:Kidney Function Tests; D008264:Macrophages / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D011507:Proteinuria / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000199:Actins; D003404:Creatinine; D018809:Proliferating Cell Nuclear Antigen; C498862:Acta2 protein, rat
keywords: Chronic renal failure progression; Mesenchymal stem cells; Stem cells
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