title: Novel methods for measuring the mitochondrial membrane potential.
abstract: The mitochondrial membrane potential is a critical parameter for understanding mitochondrial function, but it is challenging to quantitate with current methodologies which are based on the accumulation of cation indicators. Recently we have introduced a new methodology based on the redox poise of the b-hemes of the bc 1 complex. Here we describe the thermodynamic framework and algorithms necessary to calculate the membrane potential from the measured oxidation states of the b-hemes.
authors: Department of Radiology, Dartmouth Medical School, HB 7786, 1 Rope Ferry Road, Hanover, NH, 03755, USA, RSpringett@Dartmouth.edu.
authors: Roger
authors: R
authors: Springett
mesh_terms: D000465:Algorithms; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D010084:Oxidation-Reduction; D013816:Thermodynamics