title: Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.
abstract: We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function.
authors: Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors: Yao-Tong
authors: YT
authors: Lai
authors: Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors: Mayu
authors: M
authors: Sato
authors: Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Seiichi
authors: S
authors: Ohta
authors: Department of Environmental and Energy Chemistry, Kogakuin University, 2665-1 Nakano-machi, Hachioji-shi, Tokyo 192-0015, Japan.
authors: Kazuki
authors: K
authors: Akamatsu
authors: Department of Environmental and Energy Chemistry, Kogakuin University, 2665-1 Nakano-machi, Hachioji-shi, Tokyo 192-0015, Japan.
authors: Shin-ichi
authors: S
authors: Nakao
authors: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors: Yasuyuki
authors: Y
authors: Sakai
authors: Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: taichi@m.u-tokyo.ac.jp.
authors: Taichi
authors: T
authors: Ito
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D061848:Optical Imaging; D010100:Oxygen / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D033081:Shear Strength; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
chemical_list: D004655:Emulsions; D006454:Hemoglobins; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D010100:Oxygen
keywords: Albumin; Hemoglobin; Membrane emulsification; Oxygen carriers; Shirasu porous glass membrane