title: Microstructural and mechanical characterization of scarred vocal folds.
abstract: The goal of this study was to characterize the vocal folds microstructure and elasticity using nonlinear laser scanning microscopy and atomic force microscopy-based indentation, respectively. As a pilot study, the vocal folds of fourteen rats were unilaterally injured by full removal of lamina propria; the uninjured folds of the same animals served as controls. The area fraction of collagen fibrils was found to be greater in scarred tissues two months after injury than the uninjured controls. A novel mathematical model was also proposed to relate collagen concentration and tissue bulk modulus. This work presents a first step towards systematic investigation of microstructural and mechanical characteristics in scarred vocal fold tissue.
authors: Biomechanics Laboratory, Department of Mechanical Engineering, McGill University, 817 rue Sherbrooke Ouest, Montreal, QC, Canada H3A 0C3.
authors: Hossein K
authors: HK
authors: Heris
authors: Biomechanics Laboratory, Department of Mechanical Engineering, McGill University, 817 rue Sherbrooke Ouest, Montreal, QC, Canada H3A 0C3. Electronic address: amir.miriramsheh@mail.mcgill.ca.
authors: Amir K
authors: AK
authors: Miri
authors: Physics and Chemistry Departments, McGill University, 3600 rue University, Montreal, QC, Canada H3A 2T8.
authors: Nageswara R
authors: NR
authors: Ghattamaneni
authors: School of Communication Sciences and Disorders, McGill University, 2001 McGill College, Montreal, QC, Canada H3A 1G1.
authors: Nicole Y K
authors: NYK
authors: Li
authors: Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin, Madison, WI 53792, United States.
authors: Susan L
authors: SL
authors: Thibeault
authors: Physics and Chemistry Departments, McGill University, 3600 rue University, Montreal, QC, Canada H3A 2T8.
authors: Paul W
authors: PW
authors: Wiseman
authors: Biomechanics Laboratory, Department of Mechanical Engineering, McGill University, 817 rue Sherbrooke Ouest, Montreal, QC, Canada H3A 0C3.
authors: Luc
authors: L
authors: Mongeau
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D002921:Cicatrix / Q000473:pathology* / Q000503:physiopathology*; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure; D004548:Elasticity / Q000502:physiology*; D008297:Male; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D023421:Models, Animal; D008954:Models, Biological; D008962:Models, Theoretical; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014827:Vocal Cords / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D003094:Collagen
keywords: Atomic force microscopy; Scarring; Second harmonic generation; Vocal fold
references: J Voice. 2012 Nov;26(6):688-97
references: 22483778
references: J Voice. 2006 Mar;20(1):110-20
references: 15964741
references: Res Vet Sci. 1992 Nov;53(3):354-9
references: 1465509
references: J Speech Lang Hear Res. 2009 Aug;52(4):1008-20
references: 19641079
references: Otolaryngol Head Neck Surg. 1996 Nov;115(5):474-82
references: 8903451
references: J Speech Lang Hear Res. 2003 Aug;46(4):919-32
references: 12959470
references: J Voice. 2011 Mar;25(2):254-7
references: 20171830
references: Laryngoscope. 2012 Feb;122(2):356-63
references: 22252839
references: J Mech Behav Biomed Mater. 2013 Dec;28:383-92
references: 23829979
references: Acta Biomater. 2013 Aug;9(8):7957-67
references: 23643604
references: Appl Opt. 1991 Apr 10;30(11):1369-73
references: 20700292
references: Ann Otol Rhinol Laryngol. 2005 Mar;114(3):183-91
references: 15825566
references: J Voice. 2011 Jan;25(1):1-7
references: 20171829
references: J Biomech. 2000 Jun;33(6):645-52
references: 10807984
references: J Biomech. 2006;39(9):1563-82
references: 16000201
references: Ann Otol Rhinol Laryngol. 2006 Apr;115(4):285-92
references: 16676825