title: Suppression of 7,12-dimethylbenz(a)anthracene-induced mammary tumorigenesis by glyceollins.
abstract: METHODS
  Our previous study showed that glyceollins induced phase 2 detoxifying and antioxidant enzymes. As chemical carcinogens can be detoxified by metabolic activity of phase 2 enzymes, subsequently decreasing their tumorigenic potential, we investigated whether 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumorigenesis could be attenuated by treatment with glyceollins in a mouse model.
  
  
  RESULTS
  Pretreatment with glyceollins (5 mg/kg body weight) caused a significant reduction in tumor formation and an increase in survival rate. The protective effect of glyceollins against DMBA-induced tumorigenesis was found to be mainly associated with their potential to induce phase 2/antioxidant enzymes that are, in turn, regulated by the nuclear factor E2-related factor 2 signaling pathway, and to a less extent to suppress phase 1 enzymes. As glyceollins increased the number of terminal end buds, a tumor-prone mammary tissue type in mice, it is unlikely that they exert their antitumorigenic action through their estrogenic activity.
  
  
  CONCLUSIONS
  Glyceollins were found to have a protective effect in a chemically induced mammary tumor model.
authors: School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
authors: Bo Ram
authors: BR
authors: Kim
authors: Ji-Yeon
authors: JY
authors: Seo
authors: Mi-Kyung
authors: MK
authors: Sung
authors: Jung Han Yoon
authors: JH
authors: Park
authors: Hwa-Jin
authors: HJ
authors: Suh
authors: Kwang Hyeon
authors: KH
authors: Liu
authors: Jong-Sang
authors: JS
authors: Kim
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D018396:Mucin-1 / Q000032:analysis; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D036343:Pterocarpans / Q000494:pharmacology*
chemical_list: D018396:Mucin-1; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D036343:Pterocarpans; C577056:muc1 protein, mouse; D015127:9,10-Dimethyl-1,2-benzanthracene; C017343:glyceollin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D019363:Cytochrome P-450 CYP1A1; D003849:Deoxyguanosine
keywords: DMBA; Glyceollins; Mammary tumor; Nrf2; Phase 2 detoxifying enzymes