title: Low-level laser therapy attenuates the myeloperoxidase activity and inflammatory mediator generation in lung inflammation induced by gut ischemia and reperfusion: a dose-response study.
abstract: BACKGROUND
  Intestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). Herein we evaluate the dose-response effect of low-level laser therapy (LLLT) on lung inflammation induced by i-I/R.
  
  
  METHODS
  Mice were subjected to mesenteric artery occlusion (45 min) and killed after clamp release and intestinal reperfusion (2h). Increasing doses (1, 3, 5 and 7,5 J/cm(2)) of laser irradiation (660 nm) was carried out on the mice skin over the upper bronchus for 5 min after initiating reperfusion. Neutrophils activation was determined by myeloperoxidase (MPO) activity. The mRNA expression and protein concentration of inflammatory mediators IL-1β, IL-6, TNF and IL-10 in lung were measured by RT-PCR and ELISA, respectively.
  
  
  RESULTS
  With exception of 1J/cm(2), LLLT reduced MPO activity as well as IL-1β levels in the lungs from inflamed mice. LLLT was also markedly effective in reducing both IL-6 and TNF expression and levels in the lungs from mice submitted to i-I/R in all laser doses studied. Otherwise, LLLT significantly increased the protein levels of IL-10 in inflamed mice by i-I/R; however only in the dose of 1J/cm(2).
  
  
  CONCLUSIONS
  We conclude that the LLLT is able to control the neutrophils activation and proinflammatorycytokines release into the lungs in a model of i-I/R in mice.
authors: Department of Immunology, University of São Paulo, ICB, Av. Professor Lineu Prestes, 1730, Butantã, São Paulo, SP, Brazil.
authors: Flávia Mafra
authors: FM
authors: de Lima
authors: Unifesp- ICT- Instituto de Ciências e Tecnologia, Rua Talim, 330- Jardim Aeroporto, São José dos Campos, SP, Brazil.
authors: Flávio
authors: F
authors: Aimbire
authors: Federal University Rio de Janeiro, Av. Carlos Chagas Filho, 540, Rio de Janeiro, RJ, Brazil.
authors: Humberto
authors: H
authors: Miranda
authors: Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil.
authors: Rodolfo de Paula
authors: RP
authors: Vieira
authors: Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil.
authors: Ana Paula Ligeiro
authors: AP
authors: de Oliveira
authors: Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil.
authors: Regiane
authors: R
authors: Albertini
keywords: Acute; doseresponse; inflammatory mediators; laser therapy; mice; respiratory distress syndrome
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