title: Antioxidant properties and evaluation of phytochemical composition of Salvia verbenaca L. extracts at different developmental stages.
abstract: Changes in the contents and composition of polyphenolics and resulting antioxidant activities of S. verbenaca by-products were investigated at three phenological stages (flowering, early fruiting and late fruiting stages). The highest accumulation of total phenolics was detected at the flowering stage (58.36 mg GAE/g DW). HPLC analysis of methanolic extracts showed the prevalence of methyl carnosate (821.45-919.82 μg/g DW) and rosmarinic acid (544.51-649.26 μg/g DW). Phenolic diterpenes (1056.90-1148.42 μg/g DW) was the most represented class of compounds. Three complementary tests namely, DPPH(•) (IC50 value, 49.22 μg/mL) and ABTS(•+)-radical scavenging assays (146.86 μM TE/mg) and FRAP reducing power test (188.93 mM Fe(II)/mg) were used to evaluate the antioxidant capacity and showed the best performance at the early fruiting period. The current study evidenced the significant effect of phenophase on antioxidants and contributed to valorize S. verbenaca extracts as a source of functional phenolic compounds.
authors: Laboratoire de Biochimie et Biologie moléculaire, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia, mounabfarhat@yahoo.fr.
authors: Mouna
authors: M
authors: Ben Farhat
authors: Rym
authors: R
authors: Chaouch-Hamada
authors: Jose A
authors: JA
authors: Sotomayor
authors: Ahmed
authors: A
authors: Landoulsi
authors: María J
authors: MJ
authors: Jordán
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D004224:Diterpenes / Q000032:analysis; D035264:Flowers / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D027544:Salvia / Q000737:chemistry*; D013329:Structure-Activity Relationship; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D004224:Diterpenes; D010636:Phenols; D010936:Plant Extracts; D000097123:Rosmarinic Acid; C582746:methyl carnosate
references: Plant Foods Hum Nutr. 2009 Dec;64(4):303-11
references: 19904611
references: Free Radic Biol Med. 1999 May;26(9-10):1231-7
references: 10381194
references: Food Chem. 2013 Jan 1;136(1):120-9
references: 23017402
references: Food Chem. 2011 Jul 15;127(2):427-33
references: 23140682
references: Plant Foods Hum Nutr. 2012 Sep;67(3):242-6
references: 22850976
references: J Agric Food Chem. 2002 Nov 6;50(23 ):6882-90
references: 12405792
references: Bioresour Technol. 2008 Apr;99(6):1584-8
references: 17531471
references: Plant Foods Hum Nutr. 2007 Dec;62(4):151-5
references: 17912643
references: J Chromatogr A. 2006 Jul 7;1120(1-2):221-9
references: 16563403
references: Nat Prod Rep. 2003 Feb;20(1):119-36
references: 12636087
references: Plant Foods Hum Nutr. 2008 Jun;63(2):47-52
references: 18224444
references: Anal Biochem. 1996 Jul 15;239(1):70-6
references: 8660627
references: Plant Cell. 1995 Jul;7(7):1085-1097
references: 12242399
references: Plant Physiol. 2003 Apr;131(4):1816-25
references: 12692341
references: Phytochemistry. 2002 Jan;59(2):117-40
references: 11809447
references: J Agric Food Chem. 2001 Nov;49(11):5165-70
references: 11714298