title: Injectable hybrid hydrogels of hyaluronic Acid crosslinked by well-defined synthetic polycations: preparation and characterization in vitro and in vivo.
abstract: An injectable hybrid hydrogel system was developed consisting of hyaluronic acid (HA) crosslinked by well-defined block copolymers of the cationic poly(2-aminoethyl methacrylate) (PAEM) and polyethylene glycol (PEG). Robust, shear-thinning hybrid hydrogel was produced by mixing HA and 4-arm star PEG-PAEM block copolymer at 1:1 charge ratio. The encapsulation and release of highly viable human mesenchymal stem cells in physiological media was demonstrated. After subcutaneous injection of the hybrid gel in mice, mild but resolvable inflammatory response was observed. This hybrid gel could serve as a model system for studying structure-function relationship of polyelectrolyte hydrogels and as a practical injectable biomaterial for medical applications.
authors: Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church Street S. E., Minneapolis, Minnesota, 55455, USA.
authors: Daisy
authors: D
authors: Cross
authors: Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church Street S. E., Minneapolis, Minnesota, 55455, USA.
authors: Xiaoze
authors: X
authors: Jiang
authors: Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church Street S. E., Minneapolis, Minnesota, 55455, USA.
authors: Weihang
authors: W
authors: Ji
authors: Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church Street S. E., Minneapolis, Minnesota, 55455, USA.
authors: Wenqing
authors: W
authors: Han
authors: Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church Street S. E., Minneapolis, Minnesota, 55455, USA. wangx504@umn.edu.
authors: Chun
authors: C
authors: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007267:Injections; D007279:Injections, Subcutaneous; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008810:Mice, Inbred C57BL; D008863:Microspheres; D008970:Molecular Weight; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000071228:Polyelectrolytes; D058105:Polymerization; D012212:Rheology; D012997:Solvents; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid; D020100:Hydrogels; D011073:Polyamines; D000071228:Polyelectrolytes; D012997:Solvents
keywords: hyaluronic acid; hybrid hydrogel; physical hydrogel; polyelectrolyte hydrogel; star block copolymer