title: Effect of platelet-rich plasma with self-assembled peptide on the rotator cuff tear model in rat.
abstract: Several trials have been carried out to improve the healing of rotator cuff tear, but their effects remain controversial. In this study, we examined the therapeutic effect of platelet-rich plasma (PRP) in combination with self-assembled peptide (SAP) on the healing of rotator cuff tear in the rat. Twenty-seven Sprague-Dawley rats, aged 15 weeks, were used for the rotator cuff tear model. The supraspinatus muscle at the insertion site was dissected and truncated, leaving the cut edge free. A week after the surgery, the rats were randomly divided into SAP, PRP, SAP-PRP and control groups and 0.2 ml SAP, PRP, SAP-PRP and saline were injected, respectively. Bonar scores and synovial inflammation grade were checked 5 weeks after the injection. Immunofluorescence staining for heat shock protein (HSP)-70 and caspase-3 was conducted. Furthermore, maximum stride length was measured before and at 2 and 4 weeks after the injection. The Bonar scores were 6.4 ± 1.8 in the SAP group, 5.9 ± 2.0 in the PRP group, 4.7 ± 1.1 in the SAP-PRP group and 8.3 ± 2.3 in the control group. There was significant difference between the SAP-PRP and control groups in post hoc multiple comparison analysis. Among four categories of Bonar scores, collagen arrangement and vascular infiltration showed improvement after SAP-PRP injection. Immunofluorescence images showed that HSP-70 and caspase-3 were much less expressed in the SAP-PRP group than in the other groups. In conclusion, SAP-PRP can be effective in healing a rotator cuff tear by enhancing the collagen arrangement and inhibiting inflammatory changes and apoptosis. Copyright © 2015 John Wiley & Sons, Ltd.
authors: Department of Physical and Rehabilitation Medicine, Samsung Medical Centre, Seoul, Republic of Korea.
authors: Sang Jun
authors: SJ
authors: Kim
authors: Department of Physical and Rehabilitation Medicine, Samsung Medical Centre, Seoul, Republic of Korea.
authors: Sang Mok
authors: SM
authors: Lee
authors: Centre for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea.|NBIT, KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea.
authors: Ji Eun
authors: JE
authors: Kim
authors: Centre for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea.|NBIT, KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea.|Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Korea.
authors: Soo Hyun
authors: SH
authors: Kim
authors: Centre for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Korea.
authors: Youngmee
authors: 0000-0002-4778-4629
authors: Y
authors: Jung
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D001522:Behavior, Animal; D053148:Caspase 3 / Q000378:metabolism; D003094:Collagen / Q000737:chemistry; D004195:Disease Models, Animal; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007249:Inflammation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017006:Rotator Cuff / Q000601:surgery; D000070636:Rotator Cuff Injuries / Q000601:surgery*; D014945:Wound Healing
chemical_list: D018840:HSP70 Heat-Shock Proteins; D020100:Hydrogels; D003094:Collagen; C505410:Casp3 protein, rat; D053148:Caspase 3