title: In situ fluorescence labelling of jasmonic acid binding sites in plant tissues with cadmium-free quantum dots.
abstract: The fluorescence labelling of plant hormone binding sites is an important analytical technique in research on the molecular mechanisms of plant hormone activities. The authors synthesised a jasmonic acid (JA)-conjugated ZnS:Mn quantum dot (QD) probe, with a cubic structure and average hydrodynamic sizes of about 17.0 nm. The maximum fluorescence emission of the probe was recorded at about 585 nm. The probe was used for fluorescence labelling of JA binding sites in mung bean seedling tissues. Analysis revealed that the probe exhibited high selectivity to JA binding sites and good performance in eliminating interference from background fluorescence in plant tissues. In addition, the probe did not exhibit any apparent biotoxicity, and is much more suitable than probes constructed from CdTe QDs for the analysis of biological samples.
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China.
authors: Qiumei
authors: Q
authors: Liao
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China. yuyhs@scnu.edu.cn.
authors: Ying
authors: Y
authors: Yu
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China.
authors: Yujuan
authors: Y
authors: Cao
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China.
authors: Bixia
authors: B
authors: Lin
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China.
authors: Jingjing
authors: J
authors: Wei
mesh_terms: D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D003517:Cyclopentanes / Q000737:chemistry* / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008274:Magnesium; D054883:Oxylipins / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D012639:Seeds / Q000187:drug effects / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013440:Sulfides; D017967:Zinc Compounds
chemical_list: D001672:Biocompatible Materials; D003517:Cyclopentanes; D005456:Fluorescent Dyes; D054883:Oxylipins; D013440:Sulfides; D017967:Zinc Compounds; C011006:jasmonic acid; D008274:Magnesium; C031238:zinc sulfide