title: miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner.
abstract: Trefoil factor 1 (TFF1), a member of the trefoil peptide family, is not only associated with mucosal protection and restoration but is also correlated with tumorigenesis of the gastrointestinal tract. In an early study, we performed sequence analysis and identified one potential miR423-5p binding site within the 3'-untranslated region of TFF1 using microRNA target prediction tools. In the current study, we demonstrated that the coding DNA region within TFF1 is also a candidate for miR218-5p targeting. We used real-time PCR and in situ hybridization to analyze the correlation between miR218-5p and TFF1 expression in tumor lesions and paracancerous tissue in gastric cancer (GC) samples. Additionally, endogenous and exogenous TFF1 were suppressed by miR218-5p in gastric cancer cells and influenced the progression of GC in an Erk1/2-dependent manner. Targeting miR218-5p may provide a novel strategy for the treatment of GC.
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Ying
authors: Y
authors: Shi
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China; Department of Gastroenterology, Xiamen Zhongshan Teaching Hospital of Fujian Medical University, Xiamen 361004, Fujian, China.
authors: Guo-Bin
authors: GB
authors: Chen
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Qing-Wen
authors: QW
authors: Huang
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Xu
authors: X
authors: Chen
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Jing-Jing
authors: JJ
authors: Liu
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Wei
authors: W
authors: Xu
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Xiao-Xiao
authors: XX
authors: Huang
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Yun-Peng
authors: YP
authors: Liu
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China.
authors: Chuan-Xing
authors: CX
authors: Xiao
authors: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City 807, Taiwan; Division of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung City 812, Taiwan.
authors: Deng-Chyang
authors: DC
authors: Wu
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China; Faculty of Clinical Medicine, Medical College of Xiamen University, 168 University Road, Xiamen 361005, Fujian, China; State Key Laboratory of Cellular Stress Biology, Xiamen University, 168 University Road, Xiamen 361005, Fujian, China. Electronic address: bayasi8@gmail.com.
authors: Bayasi
authors: B
authors: Guleng
authors: Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian, China. Electronic address: renjianl@xmu.edu.cn.
authors: Jian-Lin
authors: JL
authors: Ren
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous; D000071163:Trefoil Factor-1; D047368:Tumor Burden / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C547471:MIRN218 microRNA, human; D035683:MicroRNAs; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Coding region; Erk1/2; Gastric cancer; TFF1; miR218-5p