title: Role of macrophage migration inhibitory factor (MIF) in pollen-induced allergic conjunctivitis and pollen dermatitis in mice.
abstract: Pollen is a clinically important airborne allergen and one of the major causes of allergic conjunctivitis. A subpopulation of patients with atopic dermatitis (AD) are also known to have exacerbated skin eruptions on the face, especially around the eyelids, after contact with pollen. This pollen-induced skin reaction is now known as pollen dermatitis. Macrophage migration inhibitory factor (MIF) is a pluripotent cytokine that plays an essential role in allergic inflammation. Recent findings suggest that MIF is involved in several allergic disorders, including AD. In this study, MIF knockout (KO), MIF transgenic (Tg) and WT littermate mice were immunized with ragweed (RW) pollen or Japanese cedar (JC) pollen and challenged via eye drops. We observed that the numbers of conjunctiva- and eyelid-infiltrating eosinophils were significantly increased in RW and JC pollen-sensitized MIF Tg compared with WT mice or MIF KO mice. The mRNA expression levels of eotaxin, interleukin (IL)-5 and IL-13 were increased in pollen-sensitized eyelid skin sites of MIF Tg mice. An in vitro analysis revealed that high eotaxin expression was induced in dermal fibroblasts by MIF combined with stimulation of IL-4 or IL-13. This eotaxin expression was inhibited by the treatment with CD74 siRNA in fibroblasts. These findings indicate that MIF can induce eosinophil accumulation in the conjunctiva and eyelid dermis exposed to pollen. Therefore, targeted inhibition of MIF might result as a new option to control pollen-induced allergic conjunctivitis and pollen dermatitis.
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors: Yuka
authors: Y
authors: Nagata
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors: Yoko
authors: Y
authors: Yoshihisa
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors: Kenji
authors: K
authors: Matsunaga
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors: Mati Ur
authors: MU
authors: Rehman
authors: Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan.
authors: Nobuyoshi
authors: N
authors: Kitaichi
authors: Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan.
authors: Nobuyuki
authors: N
authors: Kitaichi
authors: Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors: Tadamichi
authors: T
authors: Shimizu
mesh_terms: D031164:Ambrosia / Q000276:immunology; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D003233:Conjunctivitis, Allergic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D032485:Cryptomeria / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D003872:Dermatitis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008263:Macrophage Migration-Inhibitory Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011058:Pollen / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D014162:Transfection; D014611:Vaccination
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D008263:Macrophage Migration-Inhibitory Factors; D034741:RNA, Small Interfering; C045084:invariant chain
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