title: Microbial community shifts on an anammox reactor after a temperature shock using 454-pyrosequencing analysis.
abstract: To explore the changes in the microbial community structure during the recovery process of an anammox reactor after a temperature shock, the 454-pyrosequencing technique was used. The temperature shock reduced the nitrogen removal rate up to 92% compared to that just before the temperature shock, and it took 70 days to recover a similar nitrogen removal rate to that before the temperature shock (ca. 0.30 g N L(-1) d(-1)). Pyrosequencing results indicated that microbial diversity in the reactor decreased as the reactor progressively recovered from the temperature shock. Anammox bacteria were accounted as 6%, 35% and 46% of total sequence reads in samples taken 13, 45 and 166 days after the temperature shock. These results were in agreement with N-removal performance results and anammox activity measured in the reactor during the recovery process. An anammox specific primer was used to precisely determine the anammox species in the biomass samples.
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors: Eduardo
authors: E
authors: Isanta
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors: Tercia
authors: T
authors: Bezerra
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors: Isaac
authors: I
authors: Fernández
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors: María Eugenia
authors: ME
authors: Suárez-Ojeda
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors: Julio
authors: J
authors: Pérez
authors: GENOCOV Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Spain. Electronic address: julian.carrera@uab.cat.
authors: Julián
authors: J
authors: Carrera
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D064751:Ammonium Compounds / Q000378:metabolism; D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D009573:Nitrites; D000641:Ammonia; D009584:Nitrogen
keywords: 454-Pyrosequencing; Brocadia anammoxidans; Candidatus Kuenenia; Microbial diversity