title: A Cotton MYB Transcription Factor, GbMYB5, is Positively Involved in Plant Adaptive Response to Drought Stress.
abstract: Drought stress negatively affects plant growth and limits plant productivity. Genes functioning in plant responses to drought stress are essential for the development of drought-tolerant crops. Here, we report that an R2R3-type MYB transcription factor gene in Gossypium barbadense, GbMYB5, confers drought tolerance in cotton and transgenic tobacco. Virus-induced gene silencing of GbMYB5 compromised the tolerance of cotton plantlets to drought stress and reduced the post-rewatering water recovery survival rate to 50% as compared with the 90% survival rate in the wild type (WT). Silencing GbMYB5 decreased proline content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under drought stress. The expression levels of drought-inducible genes NCED3, RD22 and RD26 were not affected by the silencing of GbMYB5. However, GbMYB5-overexpressing tobacco lines displayed hypersensitivity to ABA and improved survival rates as well as reduced water loss rates under drought stress. Furthermore, stomatal size and the rate of opening of stomata were markedly decreased in transgenic tobacco. The overexpression of GbMYB5 enhanced the accumulation of proline and antioxidant enzymes while it reduced production of MDA in transgenic tobacco as compared with the WT under drought stress. The transcript levels of the antioxidant genes SOD, CAT and GST, polyamine biosynthesis genes ADC1 and SAMDC, the late embryogenesis abundant protein-encoding gene ERD10D and drought-responsive genes NCED3, BG and RD26 were generally higher in GbMYB5-overexpressing tobacco than in the WT under drought stress. Collectively, our data suggested that GbMYB5 was positively involved in the plant adaptive response to drought stress.
authors: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China These authors contributed equally to this work.
authors: Tianzi
authors: T
authors: Chen
authors: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China These authors contributed equally to this work.
authors: Wenjuan
authors: W
authors: Li
authors: College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Xuehong
authors: X
authors: Hu
authors: College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jiaru
authors: J
authors: Guo
authors: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China zhbl2248@hotmail.com amliu@jaas.ac.cn.
authors: Aimin
authors: A
authors: Liu
authors: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China zhbl2248@hotmail.com amliu@jaas.ac.cn.
authors: Baolong
authors: B
authors: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D003890:Desiccation; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D017343:Genes, Plant; D003368:Gossypium / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D055442:Metabolome / Q000187:drug effects / Q000235:genetics; D008954:Models, Biological; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid; D011392:Proline
keywords: Drought stress; GbMYB5; Gossypium barbadense; Overexpression; Tolerance; Virus-induced gene silencing