title: Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains.
abstract: Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l(-1) during shake flask conditions. A volume-corrected concentration of 11.3 g l(-1) of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.
authors: Sector for Biotechnology and Nanomedicine, Department of Molecular Biology, SINTEF Materials and Chemistry, Trondheim, Norway.
authors: Ingemar
authors: I
authors: Naerdal
authors: Johannes
authors: J
authors: Pfeifenschneider
authors: Trygve
authors: T
authors: Brautaset
authors: Volker F
authors: VF
authors: Wendisch
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000378:metabolism*; D002103:Cadaverine / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D060847:Metabolic Engineering; D000432:Methanol / Q000378:metabolism*; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D002103:Cadaverine; D000432:Methanol
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