title: Field distribution of END phenomenon-negative bovine viral diarrhea virus.
abstract: Field isolates of BVDV which do not show the exaltation of Newcastle disease virus (END) phenomenon (END(-)) are rarely reported. In this study, 45 BVDV field isolates from cattle in Hokkaido prefecture in Japan were analyzed by the reverse plaque formation method, the END method and observation of cytopathic effects. END(-) virus was detected in 34 of 45 isolates (75.6%), although 35 of 45 field isolates contained END phenomenon positive virus as the predominant virus population. We propose that END(-) viruses are widely distributed in the field and that it is possible that the mixture of biologically distinct BVDV correlates with the appearance of disease in infected animals.
authors: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
authors: Kaoru
authors: K
authors: Nishine
authors: Hiroshi
authors: H
authors: Aoki
authors: Yoshihiro
authors: Y
authors: Sakoda
authors: Akio
authors: A
authors: Fukusho
mesh_terms: D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology* / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000502:physiology; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000472:pathogenicity*; D007564:Japan / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000254:growth & development; D009841:Oligonucleotides / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D013737:Testis / Q000166:cytology
chemical_list: D009841:Oligonucleotides
references: Am J Vet Res. 2002 Oct;63(10):1455-63
references: 12375578
references: Vet Res. 2014;45:47
references: 24742209
references: Jpn J Microbiol. 1968 Mar;12(1):35-49
references: 4970697
references: Am J Vet Res. 1970 Oct;31(10):1787-94
references: 4319263
references: Infect Immun. 1976 Aug;14(2):332-6
references: 61176
references: Arch Virol. 1978;56(3):273-7
references: 205199
references: Vet Rec. 1984 Jun 2;114(22):535-6
references: 6087539
references: Microbiol Immunol. 1984;28(10):1163-7
references: 6097801
references: J Am Vet Med Assoc. 1986 Mar 15;188(6):618-9
references: 3957770
references: J Am Vet Med Assoc. 1987 Jun 1;190(11):1449-58
references: 3038804
references: Res Vet Sci. 1989 May;46(3):307-11
references: 2544969
references: Am J Vet Res. 1992 Nov;53(11):2157-63
references: 1334641
references: Vet Microbiol. 1993 Dec;38(1-2):173-9
references: 8128599
references: Vet Clin North Am Food Anim Pract. 1995 Nov;11(3):489-500
references: 8581859
references: J Vet Med Sci. 1997 May;59(5):361-70
references: 9192357
references: Vet Microbiol. 1999 Feb 23;65(1):75-86
references: 10068129
references: Science. 1958 Aug 15;128(3320):366
references: 13568799
references: Arch Gesamte Virusforsch. 1963 May 20;13:425-9
references: 14046088
references: Virology. 2005 Sep 30;340(2):265-76
references: 16043207
references: Vet Microbiol. 2005 Nov 30;111(1-2):35-40
references: 16263224
references: Prev Vet Med. 2005 Nov 15;72(1-2):177-81; discussion 215-9
references: 16162364
references: Prev Vet Med. 2005 Nov 15;72(1-2):189-207; discussion 215-9
references: 16213612
references: J Virol. 2009 Jan;83(2):817-29
references: 18987150
references: Biologicals. 2011 Jul;39(4):242-8
references: 21719306
references: Arch Virol. 2013 Apr;158(4):753-63
references: 23178968
references: J Virol. 2003 Jul;77(13):7645-54
references: 12805464