title: Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue.
abstract: Long-term excessive fluoride (F) intake disrupts the balance of bone deposition and remodeling activities and is linked to skeletal fluorosis. Type I collagen, which is responsible for bone stability and cell biological functions, can be damaged by excessive F ingestion. In this study, Sodium fluoride (NaF) was orally administrated to rat at 150 mg L(-1) for 60 and 120 d. We examined the effects of excessive F ingestion on the ultrastructure and collagen morphology of bone in rats by using transmission electron microscopy (TEM). Furthermore, we investigated the effect of F consumption on the expression levels of COL1A1 and COL1A2 in the bone tissues of rats by using quantitative real time (qRT)-PCR, to elucidate the molecular mechanisms of F-induced collagen protein damage. Our results showed that F affected collagen I arrangement and produced ultrastructural changes in bone tissue. Meanwhile, the mRNA expression of COL1A1 and COL1A2 were reduced and the COL I protein levels decreased in the fluorosis group. We concluded that excessive F ingestion adversely affected collagen I arrangement and caused ultrastructural changes in bone tissue. Reduced COL1A1 mRNA expression and altered COL I protein levels may contribute to the skeletal damage resulting from F exposure.
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China; Department of Biology, Taiyuan Normal University (TYNU), Taiyuan 030031, People's Republic of China.
authors: Xiaoyan
authors: X
authors: Yan
authors: Department of Biology, Taiyuan Normal University (TYNU), Taiyuan 030031, People's Republic of China.
authors: Xianhui
authors: X
authors: Hao
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China.
authors: Qingli
authors: Q
authors: Nie
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China.
authors: Cuiping
authors: C
authors: Feng
authors: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471003, People's Republic of China.
authors: Hongwei
authors: H
authors: Wang
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China.
authors: Zilong
authors: Z
authors: Sun
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China.
authors: Ruiyan
authors: R
authors: Niu
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China. Electronic address: wangjd@sxau.edu.cn.
authors: Jundong
authors: J
authors: Wang
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D005459:Fluorides / Q000633:toxicity*; D005786:Gene Expression Regulation; D008297:Male; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D005459:Fluorides
keywords: Fluoride; Hard tissue; Rat; Type I collagen; Ultrastructure