title: Cellular imaging using temporally flickering nanoparticles.
abstract: Utilizing the surface plasmon resonance effect in gold nanoparticles enables their use as contrast agents in a variety of applications for compound cellular imaging. However, most techniques suffer from poor signal to noise ratio (SNR) statistics due to high shot noise that is associated with low photon count in addition to high background noise. We demonstrate an effective way to improve the SNR, in particular when the inspected signal is indistinguishable in the given noisy environment. We excite the temporal flickering of the scattered light from gold nanoparticle that labels a biological sample. By preforming temporal spectral analysis of the received spatial image and by inspecting the proper spectral component corresponding to the modulation frequency, we separate the signal from the wide spread spectral noise (lock-in amplification).
authors: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors: Tali
authors: T
authors: Ilovitsh
authors: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors: Yossef
authors: Y
authors: Danan
authors: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors: Rinat
authors: R
authors: Meir
authors: Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah, USA.
authors: Amihai
authors: A
authors: Meiri
authors: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
authors: Zeev
authors: Z
authors: Zalevsky
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods* / Q000592:standards; D006046:Gold; D008546:Melanoma, Experimental; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D059629:Signal-To-Noise Ratio
chemical_list: D006046:Gold
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