title: Mitochondrial, acidic, and cytosolic pHs determination by ³¹P NMR spectroscopy: design of new sensitive targeted pH probes.
abstract: (31)P nuclear magnetic resonance (NMR) is a unique technique to monitor noninvasively the energetics of living systems at real time through the detection of a variety of phosphorylated metabolites. Using adequately designed α-aminophosphonates as external probes, we have shown earlier that (31)P NMR can also give access simultaneously to the accurate pH of cytosolic and acidic compartments in normal and stressed cultured cells or isolated perfused organs, a feature that was not possible using endogenous inorganic phosphate as the probe. More recently, we obtained a series of derivatives of these new pH probes that incorporate a triphenylphosphonium cation as a specific vector to the mitochondrion. Here, we describe the synthesis, (31)P NMR pH titrating properties in buffers, and application in cultures of the green alga Chlamydomonas reinhardtii of two of these mitochondria-targeted pH probes in comparison with one nonvectorized, yet still informative α-aminophosphonate.
authors: UMR 7273, CNRS, Equipe Sondes Moléculaires en Biologie et Stress Oxydant, Centre scientifique de Saint-Jérôme, Institut de Chimie Radicalaire, Aix-Marseille Université, Service 522, Avenue Escadrille Normandie-Niemen, Marseille Cedex 20, 13397, France.
authors: Marcel
authors: M
authors: Culcasi
authors: Sophie
authors: S
authors: Thétiot-Laurent
authors: Ariane
authors: A
authors: Atteia
authors: Sylvia
authors: S
authors: Pietri
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*