title: Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary.
abstract: In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.
authors: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P.O. Box 18, 1581, Budapest, Hungary, adoszpoly@vmri.hu.
authors: Andor
authors: A
authors: Doszpoly
authors: Melitta
authors: M
authors: Papp
authors: Petra P
authors: PP
authors: Deákné
authors: Róbert
authors: R
authors: Glávits
authors: Krisztina
authors: K
authors: Ursu
authors: Ádám
authors: Á
authors: Dán
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D016000:Cluster Analysis; D003530:Cyprinidae / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000401:mortality / Q000473:pathology / Q000821:virology*; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D006651:Histocytochemistry; D006814:Hungary / Q000453:epidemiology; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins