title: Ultrasensitive SERS detection of VEGF based on a self-assembled Ag ornamented-AU pyramid superstructure.
abstract: For the first time, we demonstrated the fabrication of silver nanoparticle ornamented-gold nanoparticle pyramids (Ag-Au Pys) using an aptamer-based self-assembly process and investigated their surface-enhanced Raman scattering (SERS) properties in the detection of vascular endothelial growth factor (VEGF). Under optimized conditions, the SERS signal was negatively related to VEGF concentration over the range 0.01-1.0 fM and the limit of detection (LOD) was as low as 22.6 aM. The matrix effect and the specificity of this developed method were further examined, and the results showed that the superstructure sensor was ultrasensitive and highly selective. This developed aptamer-based SERS detection method suggests that it may be a promising strategy for a variety of sensing applications.
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Sen
authors: S
authors: Zhao
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Wei
authors: W
authors: Ma
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China. Electronic address: xuliguang2006@126.com.
authors: Liguang
authors: L
authors: Xu
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Xiaoling
authors: X
authors: Wu
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Hua
authors: H
authors: Kuang
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Libing
authors: L
authors: Wang
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors: Chuanlai
authors: C
authors: Xu
mesh_terms: D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D042461:Vascular Endothelial Growth Factor A / Q000302:isolation & purification*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D012834:Silver; D006046:Gold
keywords: Detection; Pyramid; Self-assembly; Surface-enhanced Raman scattering; Ultrasensitive; Vascular endothelial growth factor