title: Transcriptome analysis of sexually dimorphic Chinese white wax scale insects reveals key differences in developmental programs and transcription factor expression.
abstract: The Chinese white wax scale insect, Ericerus pela, represents one of the most dramatic examples of sexual dimorphism in any insect species. In this study, we showed that although E. pela males display complete metamorphosis similar to holometabolous insects, the species forms the sister group to Acyrthosiphon pisum and cluster with hemimetabolous insects. The gene expression profile and Gene Ontology (GO) analyses revealed that the two sexes engaged in distinct developmental programs. In particular, female development appeared to prioritize the expression of genes related to cellular, metabolic, and developmental processes and to anatomical structure formation in nymphs. By contrast, male nymphal development is characterized by the significant down-regulation of genes involved in chitin, the respiratory system, and neurons. The wing and appendage morphogenesis, anatomical and tissue structure morphogenesis programs activated after male nymphal development. Transcription factors (that convey juvenile hormone or ecdysone signals, and Hox genes) and DNA methyltransferase were also differentially expressed between females and males. These results may indicate the roles that these differentially expressed genes play in regulating sexual dimorphism through orchestrating complex genetic programs. This differential expression was particularly prominent for processes linked to female development and wing development in males.
authors: Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China.
authors: Pu
authors: P
authors: Yang
authors: Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China.
authors: Xiao-Ming
authors: XM
authors: Chen
authors: Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China.
authors: Wei-Wei
authors: WW
authors: Liu
authors: Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China.
authors: Ying
authors: Y
authors: Feng
authors: Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming, 650224, China.
authors: Tao
authors: T
authors: Sun
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049750:Genome, Insect; D006430:Hemiptera / Q000235:genetics* / Q000254:growth & development*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D012727:Sex Characteristics; D014157:Transcription Factors / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics
chemical_list: D019476:Insect Proteins; D014157:Transcription Factors
references: Insect Biochem Mol Biol. 2014 Feb;45:69-76
references: 24361539
references: Sci Rep. 2014;4:4088
references: 24526031
references: Insect Mol Biol. 2004 Aug;13(4):407-12
references: 15271213
references: BMC Bioinformatics. 2004 Aug 19;5:113
references: 15318951
references: Science. 1997 Apr 4;276(5309):114-7
references: 9082981
references: Genome Res. 1997 Oct;7(10):986-95
references: 9331369
references: Chromosoma. 2004 Dec;113(6):284-94
references: 15503092
references: Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4252-7
references: 15755808
references: Bioinformatics. 2005 Oct 1;21(19):3787-93
references: 15817693
references: Dev Biol. 2005 Nov 15;287(2):440-55
references: 16183053
references: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D572-80
references: 16381935
references: Cytogenet Genome Res. 2006;113(1-4):41-52
references: 16575162
references: Genome Res. 2006 Nov;16(11):1334-8
references: 17065606
references: J Cell Sci. 2007 Mar 15;120(Pt 6):1072-80
references: 17327272
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D480-4
references: 18077471
references: Nat Methods. 2008 Jul;5(7):621-8
references: 18516045
references: Annu Rev Entomol. 2009;54:285-302
references: 19067633
references: Insect Biochem Mol Biol. 2009 Apr;39(4):254-62
references: 19168135
references: Dev Biol. 2009 May 1;329(1):142-51
references: 19382295
references: Bioinformatics. 2009 Aug 1;25(15):1966-7
references: 19497933
references: Dev Biol. 2009 Oct 1;334(1):300-10
references: 19580803
references: FEBS J. 2009 Nov;276(21):6128-57
references: 19796154
references: PLoS Comput Biol. 2009 Dec;5(12):e1000598
references: 20011106
references: Nature. 2010 Jan 21;463(7279):311-7
references: 20010809
references: Insect Biochem Mol Biol. 2010 Mar;40(3):235-40
references: 20018241
references: Amino Acids. 2010 May;38(5):1333-42
references: 19730979
references: Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12168-73
references: 20566863
references: J Insect Physiol. 2010 Oct;56(10):1420-6
references: 20417215
references: Biol Rev Camb Philos Soc. 2010 Nov;85(4):807-28
references: 20233171
references: Curr Biol. 2010 Nov 23;20(22):R972-3
references: 21093787
references: PLoS One. 2010;5(12):e15633
references: 21209894
references: Science. 1999 Dec 10;286(5447):2179-84
references: 10591654
references: Crit Rev Eukaryot Gene Expr. 2001;11(1-3):145-71
references: 11693959
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Development. 2002 Apr;129(7):1739-50
references: 11923209
references: Science. 2002 Aug 16;297(5584):1135-6
references: 12183618
references: Proc Biol Sci. 2003 Jun 22;270(1521):1263-70
references: 12816639
references: Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4877-82
references: 15041751
references: Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2855-60
references: 21282633
references: PLoS One. 2011;6(12):e28728
references: 22174880
references: Insect Mol Biol. 2012 Feb;21(1):129-38
references: 22122805
references: Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2949-54
references: 22308414
references: PLoS One. 2012;7(2):e31045
references: 22359564
references: Trends Genet. 2012 Apr;28(4):175-84
references: 22425532
references: PLoS One. 2012;7(4):e35719
references: 22536429
references: Biochim Biophys Acta. 2013 Jan;1830(1):2178-87
references: 23041750
references: Annu Rev Entomol. 2013;58:181-204
references: 22994547
references: Sci Rep. 2013;3:1860
references: 23681267
references: Insect Sci. 2013 Apr;20(2):254-60
references: 23955865