title: Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology.
abstract: Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.
authors: Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
authors: Stephan A
authors: SA
authors: Pless
authors: Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, Vancouver, BC, Canada.
authors: Robin Y
authors: RY
authors: Kim
authors: Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.
authors: Christopher A
authors: CA
authors: Ahern
authors: Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, Vancouver, BC, Canada.
authors: Harley T
authors: HT
authors: Kurata
mesh_terms: D000596:Amino Acids / Q000235:genetics; D000818:Animals; D007473:Ion Channels / Q000737:chemistry / Q000502:physiology; D016296:Mutagenesis
chemical_list: D000596:Amino Acids; D007473:Ion Channels
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