title: Peri-Implant Bone Regeneration Using rhPDGF-BB, BMSCs, and β-TCP in a Canine Model.
abstract: BACKGROUND
  The presence of insufficient bone volume remains a major clinical problem for dental implant placement to restore oral function. Tissue engineering provides a promising approach for inducing bone regeneration and enhancing osseointegration in dental implants.
  
  
  OBJECTIVE
  The tissue-engineered bone consisting of recombinant human platelet-derived growth factor (rhPDGF-BB), bone marrow stem cells (BMSCs), and beta-tricalcium phosphate (β-TCP) particles was validated for the first time in a preclinical large animal canine model in terms of its ability to promote new bone formation around the implants, as well as osseointegration between the tissue-engineered bone and dental implants.
  
  
  METHODS
  Proliferation and osteogenic differentiation of canine BMSCs treated with rhPDGF-BB were evaluated with an MTT, alkaline phosphatase (ALP) activity, Alizarin Red staining, and real-time quantitative PCR (RT-qPCR) analysis of osteogenic genes. The therapeutic potential of tissue-engineered bone consisting of rhPDGF-BB/BMSCs/β-TCP in bone repair was evaluated in mesial-implant defects of immediate postextraction implants in the canine mandible.
  
  
  RESULTS
  rhPDGF-BB treatment significantly increased proliferation and osteogenic differentiation of canine BMSCs. Furthermore, the tissue-engineered bone consisting of rhPDGF-BB/BMSCs/β-TCP significantly enhanced bone formation and osseointegration.
  
  
  CONCLUSIONS
  This study provides important evidence that supports the potential application of rhPDGF-BB/BMSCs/β-TCP tissue-engineered bone in immediate implantation for oral function restoration.
authors: Department of Prosthodontics, School of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Ling
authors: L
authors: Xu
authors: Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Wenjie
authors: W
authors: Zhang
authors: Department of Prosthodontics, School of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Kaige
authors: K
authors: Lv
authors: Department of Prosthodontics, School of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Weiqiang
authors: W
authors: Yu
authors: Department of Prosthodontics, School of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Xinquan
authors: X
authors: Jiang
authors: Department of Prosthodontics, School of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Fuqiang
authors: F
authors: Zhang
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002130:Calcium Phosphates; D015921:Dental Implants; D004285:Dogs; D006801:Humans; D008334:Mandible; D016348:Osseointegration; D010012:Osteogenesis; D010982:Platelet-Derived Growth Factor; D013234:Stem Cells
chemical_list: D002130:Calcium Phosphates; D015921:Dental Implants; D010982:Platelet-Derived Growth Factor; C485817:beta-tricalcium phosphate
keywords: BMSCs; immediate implantation; rhPDGF-BB; tissue engineering; β-TCP