title: Recombinase and translesion DNA polymerase decrease the speed of replication fork progression during the DNA damage response in Escherichia coli cells.
abstract: The SOS response is a DNA damage response pathway that serves as a general safeguard of genome integrity in bacteria. Extensive studies of the SOS response in Escherichia coli have contributed to establishing the key concepts of cellular responses to DNA damage. However, how the SOS response impacts on the dynamics of DNA replication fork movement remains unknown. We found that inducing the SOS response decreases the mean speed of individual replication forks by 30-50% in E. coli cells, leading to a 20-30% reduction in overall DNA synthesis. dinB and recA belong to a group of genes that are upregulated during the SOS response, and encode the highly conserved proteins DinB (also known as DNA polymerase IV) and RecA, which, respectively, specializes in translesion DNA synthesis and functions as the central recombination protein. Both genes were independently responsible for the SOS-dependent slowdown of replication fork progression. Furthermore, fork speed was reduced when each gene was ectopically expressed in SOS-uninduced cells to the levels at which they are expressed in SOS-induced cells. These results clearly indicate that the increased expression of dinB and recA performs a novel role in restraining the progression of an unperturbed replication fork during the SOS response.
authors: Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors: Kang Wei
authors: KW
authors: Tan
authors: Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors: Tuan Minh
authors: TM
authors: Pham
authors: Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors: Asako
authors: A
authors: Furukohri
authors: Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors: Hisaji
authors: H
authors: Maki
authors: Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan akiyamam@bs.naist.jp.
authors: Masahiro Tatsumi
authors: MT
authors: Akiyama
mesh_terms: D004249:DNA Damage; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D045522:Recombinases / Q000378:metabolism*; D013014:SOS Response, Genetics
chemical_list: D004269:DNA, Bacterial; D045522:Recombinases; D004259:DNA-Directed DNA Polymerase
references: J Bacteriol. 1995 Jan;177(1):156-65
references: 8002613
references: Bioessays. 1995 Jan;17(1):63-70
references: 7702595
references: Microbiol Mol Biol Rev. 1997 Jun;61(2):212-38
references: 9184011
references: Science. 1997 Sep 5;277(5331):1518-23
references: 9278517
references: Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13792-7
references: 9391106
references: Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9218-23
references: 10430923
references: Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3942-7
references: 15738384
references: Mol Microbiol. 2005 Sep;57(6):1664-75
references: 16135232
references: J Bacteriol. 2005 Nov;187(22):7607-18
references: 16267285
references: Mol Syst Biol. 2006;2:2006.0008
references: 16738554
references: EMBO J. 2006 Jun 7;25(11):2596-604
references: 16724111
references: J Mol Biol. 2007 Mar 30;367(3):665-80
references: 17280685
references: Cell Cycle. 2007 Feb 15;6(4):471-7
references: 17329970
references: Crit Rev Biochem Mol Biol. 2007 Jan-Feb;42(1):41-63
references: 17364684
references: Mol Cell Biol. 2007 Apr;27(8):3131-42
references: 17296725
references: Nat Genet. 2007 Jun;39(6):797-802
references: 17529976
references: Mol Cell Biol. 2007 Aug;27(16):5806-18
references: 17515603
references: Mol Cell. 2007 Dec 28;28(6):1058-70
references: 18158902
references: J Biol Chem. 2008 Apr 25;283(17):11260-9
references: 18308729
references: Cell Cycle. 2008 Aug;7(15):2315-20
references: 18677104
references: Mol Microbiol. 2008 Sep;69(6):1341-8
references: 18673457
references: Mol Microbiol. 2008 Nov;70(3):608-22
references: 18761688
references: Microbiol Mol Biol Rev. 2008 Dec;72(4):642-71, Table of Contents
references: 19052323
references: J Cell Biol. 2008 Dec 29;183(7):1203-12
references: 19103807
references: Mol Cell Biol. 2009 Jan;29(2):602-11
references: 19001087
references: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6031-8
references: 19279203
references: Genetics. 2009 May;182(1):55-68
references: 19270270
references: Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12664-9
references: 19617571
references: Mol Microbiol. 2009 Dec;74(5):1143-51
references: 19843218
references: Biochim Biophys Acta. 2010 May;1804(5):1167-79
references: 19540941
references: Mol Microbiol. 2011 Apr;80(2):524-41
references: 21320186
references: Curr Opin Chem Biol. 2011 Oct;15(5):595-605
references: 21865075
references: EMBO J. 2012 Feb 15;31(4):908-18
references: 22157819
references: Mol Cell. 2012 Mar 9;45(5):696-704
references: 22325992
references: J Bacteriol. 2012 Jul;194(14):3589-600
references: 22544274
references: Genes Genet Syst. 2012;87(2):75-87
references: 22820381
references: Nucleic Acids Res. 2012 Jul;40(13):6039-48
references: 22447448
references: J Bacteriol. 2013 Mar;195(6):1179-93
references: 23292773
references: Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5410-5
references: 23509251
references: J Cell Sci. 2013 Mar 15;126(Pt 6):1297-306
references: 23645160
references: Cold Spring Harb Perspect Biol. 2013 Oct;5(10):a010363
references: 23838442
references: Cold Spring Harb Perspect Biol. 2013 Nov;5(11):a012674
references: 24097899
references: J Mol Biol. 2013 Nov 29;425(23):4733-44
references: 23643490
references: Cold Spring Harb Perspect Biol. 2013 Dec;5(12):a012682
references: 24296168
references: Environ Mol Mutagen. 2014 Mar;55(2):92-102
references: 24243543
references: Mol Microbiol. 2013 Nov;90(3):584-96
references: 23998701
references: Nucleic Acids Res. 2014 Jun;42(10):6497-510
references: 24829446
references: J Mol Biol. 2013 Nov 29;425(23):4745-55
references: 24021813
references: Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents
references: 10585965
references: Nature. 2000 Mar 2;404(6773):37-41
references: 10716434
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
references: 10829079
references: Curr Biol. 2000 Dec 14-28;10(24):R908-11
references: 11137027
references: Genetics. 2001 May;158(1):41-64
references: 11333217
references: Mol Cell. 2001 Jul;8(1):7-8
references: 11515498
references: Mol Genet Genomics. 2001 Oct;266(2):207-15
references: 11683261
references: Genes Cells. 2003 May;8(5):437-49
references: 12694533
references: Genetics. 2003 Apr;163(4):1243-54
references: 12702672
references: Mol Cell. 2003 Apr;11(4):1109-17
references: 12718895
references: Annu Rev Genet. 2003;37:611-46
references: 14616075
references: J Biol Chem. 2004 May 14;279(20):21543-51
references: 15014081
references: Annu Rev Biochem. 2004;73:39-85
references: 15189136
references: Proc Natl Acad Sci U S A. 1967 Nov;58(5):1903-10
references: 4866981
references: J Bacteriol. 1969 Jan;97(1):244-9
references: 4884815
references: Basic Life Sci. 1975;5A:355-67
references: 1103845
references: J Bacteriol. 1981 Oct;148(1):386-90
references: 6457028
references: Proc Natl Acad Sci U S A. 1984 Dec;81(23):7539-43
references: 6390441
references: EMBO J. 1986 Jul;5(7):1711-7
references: 3527695
references: J Mol Biol. 1988 Oct 20;203(4):861-74
references: 2974887