title: Perlecan and synaptophysin changes in denervated skeletal muscle.
abstract: The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.
authors: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.
authors: Kai
authors: K
authors: Ma
authors: School of Pharmacy, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China.
authors: Zhifeng
authors: Z
authors: Huang
authors: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.
authors: Jianfeng
authors: J
authors: Ma
authors: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors: Longquan
authors: L
authors: Shao
authors: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China.
authors: Huiming
authors: H
authors: Wang
authors: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.
authors: Yanliang
authors: Y
authors: Wang
keywords: acetylcholine esterase; denervated; extracellular matrix; neural regeneration; neuromuscular junction; perlecan; sciatic nerve; skeletal muscle; synaptophysin
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