title: Simultaneous quantification of 16 bioactive constituents in Common cnidium fruit by liquid chromatography-electrospray ionization-mass spectrometry.
abstract: A novel quantitative method using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry was developed for simultaneous determination of the 16 important bioactive constituents including nine coumarins, and seven flavonoids in Common cnidium fruit samples from different regions. The separation was performed on a C18 column with linear gradient elution of acetonitrile and 0.1% acetic acid at a flow rate of 1.0 ml/min in 15 min. Quantification of the analytes was achieved by use of a hybrid quadrupole linear ion-trap mass spectrometer. Multiple-reaction monitoring scanning was employed with switching electrospray ion source polarity between positive and negative modes in a single run. The validation results of the method indicated that the method was simple, rapid, specific, and reliable. The results demonstrated that the quantitative difference in content of 16 bioactive constituents was useful not only for chemotaxonomy of many samples from different sources but also for the standardization and differentiation of many similar samples. Simultaneous quantification of bioactive components by high performance liquid chromatography-tandem mass spectrometric method would be a well acceptable strategy to comprehensively control the quality of C. cnidium fruit.
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, China; Hebei Institute for Food and Drug Control, Shijiazhuang, China.
authors: Gengshen
authors: G
authors: Song
authors: CSPC Zhongqi Pharmaceutical Technology (Shijiazhuang) Co. Ltd., Shijiazhuang, China.
authors: Li
authors: L
authors: Zhou
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, China.
authors: Ning
authors: N
authors: Sheng
authors: Hebei Institute for Food and Drug Control, Shijiazhuang, China.
authors: Xiru
authors: X
authors: Zhang
authors: Hebei Institute for Food and Drug Control, Shijiazhuang, China.
authors: Yanmei
authors: Y
authors: Xu
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, China. Electronic address: zhanglantong@263.net.
authors: Lantong
authors: L
authors: Zhang
authors: Hebei Institute for Food and Drug Control, Shijiazhuang, China.
authors: Xiaoqian
authors: X
authors: Li
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D001685:Biological Factors / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000097:Acetonitriles; D001685:Biological Factors; D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts; C032159:acetonitrile
keywords: Bioactive constituents; Common cnidium fruit; HPLC–ESI-MS/MS; Multiple-reaction monitoring; Quantification