title: Human bone marrow mesenchymal stem cell transplantation attenuates axonal injury in stroke rats.
abstract: Previous studies have shown that transplantation of human bone marrow mesenchymal stem cells promotes neural functional recovery after stroke, but the neurorestorative mechanisms remain largely unknown. We hypothesized that functional recovery of myelinated axons may be one of underlying mechanisms. In this study, an ischemia/reperfusion rat model was established using the middle cerebral artery occlusion method. Rats were used to test the hypothesis that intravenous transplantation of human bone marrow mesenchymal stem cells through the femoral vein could exert neuroprotective effects against cerebral ischemia via a mechanism associated with the ability to attenuate axonal injury. The results of behavioral tests, infarction volume analysis and immunohistochemistry showed that cerebral ischemia caused severe damage to the myelin sheath and axons. After rats were intravenously transplanted with human bone marrow mesenchymal stem cells, the levels of axon and myelin sheath-related proteins, including microtubule-associated protein 2, myelin basic protein, and growth-associated protein 43, were elevated, infarct volume was decreased and neural function was improved in cerebral ischemic rats. These findings suggest that intravenously transplanted human bone marrow mesenchymal stem cells promote neural function. Possible mechanisms underlying these beneficial effects include resistance to demyelination after cerebral ischemia, prevention of axonal degeneration, and promotion of axonal regeneration.
authors: Department of Neurosurgery, General Hospital of Chinese PLA, Beijing, China.
authors: Yi
authors: Y
authors: Xu
authors: Department of Neurosurgery, General Hospital of Armed Police Forces, Beijing, China.
authors: Shiwei
authors: S
authors: Du
authors: Department of Neurosurgery, General Hospital of Chinese PLA, Beijing, China.
authors: Xinguang
authors: X
authors: Yu
authors: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Institute of Basic Medical Sciences, Beijing, China ; Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medcal Sciences of Xuyuan Hospital, Beijing, China.
authors: Xiao
authors: X
authors: Han
authors: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Institute of Basic Medical Sciences, Beijing, China ; Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medcal Sciences of Xuyuan Hospital, Beijing, China.
authors: Jincai
authors: J
authors: Hou
authors: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Institute of Basic Medical Sciences, Beijing, China ; Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medcal Sciences of Xuyuan Hospital, Beijing, China.
authors: Hao
authors: H
authors: Guo
keywords: axon regeneration; demyelination; growth associated protein 43; human bone marrow mesenchymal stem cells; ischemic stroke; microtubule-associated protein 2; myelin basic protein; myelin sheath; nerve regeneration; neural function; neural regeneration; neuraxon; neuroprotection
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