title: Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension.
abstract: Increased endogenous αB-crystallin protein levels have been shown to reduce cell apoptosis, although the effects of exogenous αB-crystallin protein remain poorly understood. The present study established an acute ocular hypertension model in the right eye of Sprague-Dawley rats. Fluorogold retrograde tracing and immunofluorescence methods showed that the number of retinal ganglion cells decreased in the right eyes and caspase-3 expression increased following acute ocular hypertension. Intravitreal injection of αB-crystallin in the right eye increased the number of retinal ganglion cells and reduced caspase-3 expression. Results demonstrated that exogenous αB-crystallin protein inhibited caspase-3 expression and improved retinal ganglion cell survival following acute ocular hypertension.
authors: Department of Ophthalmology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors: Zhihong
authors: Z
authors: Wu
authors: Ningbo Eye Hospital, Ningbo 315000, Zhejiang Province, China.
authors: Layi
authors: L
authors: Wang
authors: Department of Ophthalmology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors: Shike
authors: S
authors: Hou
keywords: acute ocular hypertension; caspase-3; neural regeneration; retinal ganglion cells; αB-crystallin protein
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