title: Extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins.
abstract: Sulfated polysaccharides were extracted from gray triggerfish (GTSP) and smooth hound (SHSP) skins. Their chemical and physical characteristics were determined using X-ray diffraction and Infrared spectroscopic analysis. The antibacterial activities of GTSP and SHSP against Listeria monocytogenes (ATCC 43251), Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Salmonella enterica (ATCC 43972) and Enterobacter sp were evaluated by determining clear growth inhibition zone diameters and the minimum inhibitory concentration (MIC) values and by essays in liquid media. GTSP and SHSP were fractionated by a Diethylaminoethyl-cellulose chromatography. Fraction FGII, from GTSP, and fraction FSII, from SHSP, showed the most important inhibitory effects against the tested bacterial species. The sulfated polysaccharides from fish skins did not show hemolytic activity towards bovine erythrocytes. Overall, the results suggested that those polysaccharides could offer promising sources of polysaccharides for future application as dietary ingredients in the nutraceutical industry.
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Fatma
authors: F
authors: Krichen
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Wafa
authors: W
authors: Karoud
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Assaâd
authors: A
authors: Sila
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Baha Eddine
authors: BE
authors: Abdelmalek
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Raoudha
authors: R
authors: Ghorbel
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia.
authors: Semia
authors: S
authors: Ellouz-Chaabouni
authors: Enzyme and Bioconversion Unit, National School of Engineering of Sfax, University of Sfax, 3038 Sfax, Tunisia. Electronic address: ali.bougatef79@gmail.com.
authors: Ali
authors: A
authors: Bougatef
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D002852:Chromatography, Ion Exchange; D003636:DEAE-Cellulose / Q000737:chemistry; D005399:Fishes; D005658:Fungi / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000302:isolation & purification* / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D011134:Polysaccharides; D013431:Sulfates; D003636:DEAE-Cellulose
keywords: Antimicrobial activity; Fish skins; Functional foods; Pathogenic strains; Sulfated polysaccharides