title: Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine.
abstract: Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.
authors: Department of Biochemistry, Raboud Institute for Molecular Life Sciences, Radboud University Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands.
authors: Marleen
authors: M
authors: Forkink
authors: Peter H G M
authors: PH
authors: Willems
authors: Werner J H
authors: WJ
authors: Koopman
authors: Sander
authors: S
authors: Grefte
mesh_terms: D002460:Cell Line; D003997:Dicarbethoxydihydrocollidine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008853:Microscopy / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; C514241:dihydroethidine; D003997:Dicarbethoxydihydrocollidine