title: Profiling of fatty acids composition in suet oil based on GC-EI-qMS and chemometrics analysis.
abstract: Fatty acid (FA) composition of suet oil (SO) was measured by precolumn methylesterification (PME) optimized using a Box-Behnken design (BBD) and gas chromatography/electron ionization-quadrupole mass spectrometry (GC-EI-qMS). A spectral library (NIST 08) and standard compounds were used to identify FAs in SO representing 90.89% of the total peak area. The ten most abundant FAs were derivatized into FA methyl esters (FAMEs) and quantified by GC-EI-qMS; the correlation coefficient of each FAME was 0.999 and the lowest concentration quantified was 0.01 μg/mL. The range of recovery of the FAMEs was 82.1%-98.7% (relative standard deviation 2.2%-6.8%). The limits of quantification (LOQ) were 1.25-5.95 μg/L. The number of carbon atoms in the FAs identified ranged from 12 to 20; hexadecanoic and octadecanoic acids were the most abundant. Eighteen samples of SO purchased from Qinghai, Anhui and Jiangsu provinces of China were categorized into three groups by principal component analysis (PCA) according to the contents of the most abundant FAs. The results showed SOs samples were rich in FAs with significantly different profiles from different origins. The method described here can be used for quality control and SO differentiation on the basis of the FA profile.
authors: Affiliated Hospital on Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Xianlin Avenue 138#, Xianlin University City, Nanjing 210023, China. xuyan9323@126.com.
authors: Jun
authors: J
authors: Jiang
authors: Affiliated Hospital on Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Xianlin Avenue 138#, Xianlin University City, Nanjing 210023, China. xiaobinjia_nj@126.com.
authors: Xiaobin
authors: X
authors: Jia
mesh_terms: D000818:Animals; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006041:Goats; D009821:Oils / Q000737:chemistry*; D025341:Principal Component Analysis; D012756:Sheep
chemical_list: D005227:Fatty Acids; D009821:Oils
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