title: Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans.
abstract: The inner nuclear membrane proteins emerin and LEMD2 have both overlapping and separate functions in regulation of nuclear organization, gene expression and cell differentiation. We report here that emerin (EMR-1) and LEM domain protein 2 (LEM-2) are expressed in all tissues throughout Caenorhaditis elegans development but their relative distribution differs between cell types. The ratio of EMR-1 to LEM-2 is particularly high in contractile tissues, intermediate in neurons and hypodermis and lowest in intestine and germ line. We find that LEM-2 is recruited earlier than EMR-1 to reforming nuclear envelopes, suggesting the presence of separate mitotic membrane compartments and specific functions of each protein. Concordantly, we observe that nuclei of lem-2 mutant embryos, but not of emr-1 mutants, have reduced nuclear circularity. Finally, we uncover a so-far-unknown role of LEM-2 in nuclear separation and anchoring of microtubule organizing centers.
authors: Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, Carretera de Utrera, km 1, 41013 Seville, Spain.
authors: Adela
authors: A
authors: Morales-Martínez
authors: Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, Carretera de Utrera, km 1, 41013 Seville, Spain.
authors: Agnieszka
authors: A
authors: Dobrzynska
authors: Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, Carretera de Utrera, km 1, 41013 Seville, Spain pask@upo.es.
authors: Peter
authors: P
authors: Askjaer
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; C573699:EMR-1 protein, C elegans; D008565:Membrane Proteins; D009687:Nuclear Proteins; C485768:lem-2 protein, C elegans
keywords: Caenorhabditis elegans; Emerin; LEMD2; LINC; Laminopathy; Nuclear envelope