title: Improved gene transfection efficacy and cytocompatibility of multifunctional polyamidoamine-cross-linked hyaluronan particles.
abstract: We describe a multi-functional, cationic hyaluronic acid (HA)-based gene carrier with improved transfection over non-cross-linked HA, and negligible cytotoxicity. Cationized particles are developed by cross-linking HA chain carboxyl groups with polyamidoamine amine termini to produce well segregated particles of 350-400 nm with a surface charge density of +2 mV, compared with -35 mV for non-cationized particles. A tethered antibody fragment retains ligand binding for cell targeting. Cationized and antibody-linked particles complex plasmid DNA efficiently and the cationized particles successfully deliver reporter genes to bovine intervertebral disk cells as an intervertebral disk regeneration model.
authors: Network of Excellence for Functional Biomaterials, Biosciences, NUI Galway, Galway, Ireland.
authors: Akshay
authors: A
authors: Srivastava
authors: Microbiology and Network of Excellence for Functional Biomaterials, NUI Galway, University Road, Galway, Ireland.
authors: Claire
authors: C
authors: Cunningham
authors: Network of Excellence for Functional Biomaterials, Biosciences, NUI Galway, Galway, Ireland.
authors: Abhay
authors: A
authors: Pandit
authors: Microbiology and Network of Excellence for Functional Biomaterials, NUI Galway, University Road, Galway, Ireland. gerard.wall@nuigalway.ie.
authors: J Gerard
authors: JG
authors: Wall
mesh_terms: D000818:Animals; D002412:Cations; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007128:Immunoglobulin Fragments / Q000378:metabolism; D007403:Intervertebral Disc / Q000166:cytology*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D003432:Cross-Linking Reagents; D007128:Immunoglobulin Fragments; C531249:Poly(amidoamine); D011073:Polyamines; D006820:Hyaluronic Acid; D016650:Fluorescein-5-isothiocyanate
keywords: conjugated polymers; drug delivery systems; hyaluronic acid; intervertebral disk; poly(amidoamine)