title: The status of RNAi-based transgenic research in plant nematology.
abstract: With the understanding of nematode-plant interactions at the molecular level, new avenues for engineering resistance have opened up, with RNA interference being one of them. Induction of RNAi by delivering double-stranded RNA (dsRNA) has been very successful in the model non-parasitic nematode, Caenorhabditis elegans, while in plant nematodes, dsRNA delivery has been accomplished by soaking nematodes with dsRNA solution mixed with synthetic neurostimulants. The success of in vitro RNAi of target genes has inspired the use of in planta delivery of dsRNA to feeding nematodes. The most convincing success of host-delivered RNAi has been achieved against root-knot nematodes. Plant-mediated RNAi has been shown to lead to the specific down-regulation of target genes in invading nematodes, which had a profound effect on nematode development. RNAi-based transgenics are advantageous as they do not produce any functional foreign proteins and target organisms in a sequence-specific manner. Although the development of RNAi-based transgenics against plant nematodes is still in the preliminary stage, they offer novel management strategy for the future.
authors: Division of Nematology, Indian Agricultural Research Institute New Delhi, India.
authors: Tushar K
authors: TK
authors: Dutta
authors: Division of Nematology, Indian Agricultural Research Institute New Delhi, India.
authors: Prakash
authors: P
authors: Banakar
authors: Division of Nematology, Indian Agricultural Research Institute New Delhi, India.
authors: Uma
authors: U
authors: Rao
keywords: cyst nematodes; dsRNA; gene silencing; host-delivered RNAi; root-knot nematodes; siRNA
references: PLoS One. 2013 Nov 06;8(11):e80603
references: 24223228
references: Science. 2000 Mar 31;287(5462):2494-7
references: 10741970
references: Phytopathology. 2013 Feb;103(2):175-81
references: 23294405
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D720-8
references: 22139919
references: PLoS Pathog. 2013 Oct;9(10):e1003745
references: 24204279
references: Exp Parasitol. 2011 Jan;127(1):90-9
references: 20599433
references: PLoS One. 2013 Jul 16;8(7):e69463
references: 23874962
references: Planta. 2009 Jun;230(1):53-71
references: 19347355
references: Annu Rev Phytopathol. 2009;47:207-32
references: 19400649
references: Funct Plant Biol. 2006 Nov;33(11):991-999
references: 32689310
references: PLoS One. 2013 Jun 05;8(6):e66011
references: 23755297
references: Planta. 2010 Aug;232(3):775-85
references: 20582434
references: Plant Cell Rep. 2010 Feb;29(2):113-23
references: 20012965
references: Trends Plant Sci. 2005 Aug;10(8):362-7
references: 16027029
references: Mol Plant Pathol. 2007 Sep;8(5):701-11
references: 20507531
references: Mol Plant Pathol. 2013 Dec;14(9):946-61
references: 23809086
references: Trends Parasitol. 2004 Mar;20(3):134-41
references: 15036035
references: Planta. 2007 Nov;226(6):1525-33
references: 17653759
references: Exp Parasitol. 2013 Feb;133(2):166-78
references: 23201220
references: Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14302-6
references: 16985000
references: J Exp Bot. 2009;60(1):315-24
references: 19015219
references: Methods. 2003 Aug;30(4):289-95
references: 12828942
references: Int J Parasitol. 2010 Jun;40(7):855-64
references: 20100489
references: Curr Opin Biotechnol. 2012 Apr;23(2):251-6
references: 21996368
references: BMC Genomics. 2013 Oct 01;14:668
references: 24083348
references: Plant Biotechnol J. 2014 Sep;12(7):821-31
references: 25040343
references: Mol Plant Microbe Interact. 2013 Jan;26(1):97-105
references: 22857385
references: Nature. 1998 Feb 19;391(6669):806-11
references: 9486653
references: Mol Plant Microbe Interact. 2002 Aug;15(8):747-52
references: 12182331
references: Phytopathology. 2014 Oct;104(10):1098-106
references: 24835223
references: Parasitology. 2012 Apr;139(5):630-40
references: 22217302
references: PLoS Pathog. 2014 Sep 25;10(9):e1004391
references: 25255291
references: PLoS One. 2013 May 22;8(5):e64586
references: 23717636
references: Phytopathology. 2013 Nov;103(11):1092-102
references: 23777404
references: Mol Plant Microbe Interact. 1997 Apr;10(3):394-400
references: 9100383
references: Mol Biochem Parasitol. 2006 Aug;148(2):219-22
references: 16678282
references: Mol Plant Microbe Interact. 2005 Jul;18(7):621-5
references: 16042007
references: J Exp Bot. 2010;61(1):235-48
references: 19887499