title: Increased expression of MUC5AC and MUC5B promoting bacterial biofilm formation in chronic rhinosinusitis patients.
abstract: OBJECTIVE
  Binding of bacteria to mucin and then colonizing is the first step of bacterial biofilm (BBF) formation in chronic rhinosinusitis (CRS) patients. Yet, information is sparse on how mucins effects on BBF formation. In this study, we aimed to investigate the relationship between mucin expression and the formation of BBF in CRS patients.
  
  
  METHODS
  Sinus mucosa were harvested from 24 patients undergoing endoscopic surgery for CRS. The positive of BBF formation were detected by scanning electronic microscopy (SEM) and the expression of MUC1, MUC2, MUC5AC, and MUC5B were determined by immunohistochemistry (IHC). The difference expression of mucins were analyzed between the BBF positive and negative cohorts in CRS patients.
  
  
  RESULTS
  MUC5AC and MUC5B are two major mucins in CRS mucosa with the former mainly restricted to the goblet cells of epithelium and the latter mainly restricted to the submucosal glands. Expression of MUC5AC and MUC5B in the sinus mucosa of BBF(+) CRS group was significantly higher than those in BBF(-) CRS group (p<0.05). For MUC1 and MUC2, no significant difference was found between the two groups (p>0.05).
  
  
  CONCLUSIONS
  Our study indicated that increased expression of MUC5AC and MUC5B may play an important role in the pathogenesis of BBF formation.
authors: Department of Head-Neck Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors: Yong-Jun
authors: YJ
authors: Mao
authors: Department of Head-Neck Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. Electronic address: haihong_zju@hotmail.com.
authors: Hai-Hong
authors: HH
authors: Chen
authors: Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors: Bo
authors: B
authors: Wang
authors: Department of Head-Neck Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors: Xiang
authors: X
authors: Liu
authors: Department of Head-Neck Otolaryngology, TongDe Hospital of Zhejiang Province, Hangzhou 310012, China.
authors: Gao-Yun
authors: GY
authors: Xiong
mesh_terms: D000328:Adult; D000368:Aged; D018441:Biofilms; D002908:Chronic Disease; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism; D018396:Mucin-1 / Q000378:metabolism; D055262:Mucin-2 / Q000378:metabolism; D055257:Mucin-5B / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D011446:Prospective Studies; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000382:microbiology; D012220:Rhinitis / Q000378:metabolism* / Q000382:microbiology / Q000601:surgery; D012852:Sinusitis / Q000378:metabolism* / Q000382:microbiology / Q000601:surgery; D055815:Young Adult
chemical_list: C060192:MUC1 protein, human; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524578:MUC5B protein, human; D055271:Mucin 5AC; D018396:Mucin-1; D055262:Mucin-2; D055257:Mucin-5B; D009077:Mucins
keywords: Bacterial biofilm; Chronic rhinosinusitis; MUC1; MUC2; MUC5AC; MUC5B