title: Dietary supplement of banana (Musa acuminata) peels hot-water extract to enhance the growth, anti-hypothermal stress, immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii.
abstract: In the present study, Macrobrachium rosenbergii were fed with diets containing extracts of banana, Musa acuminate, fruit's peel (banana peels extract, BPE) at 0, 1.0, 3.0 and 6.0 g kg(-1). The non-specific immune parameters, disease resistance and anti-hypothermal stress were evaluated at 2, 4, 8, 16 and 32 days of post feeding. Also, we demonstrated the percent weight gain (PWG), percent length gain (PLG), feeding efficiency (FE), and survival rate of giant freshwater prawn at 30, 60, 90, and 120 days of post feeding. The PWG, PLG, FE and survival rate of prawns fed at 0, 1.0, 3.0 and 6.0 g kg(-1) BPE-containing diets after 120 days were 69.5%, 75.4%, 77.8% and 83.3%; 21.8%, 23.6%, 27.8% and 33.9%; 0.60, 0.72, 0.75 and 0.90; and 55.4%, 62.2%, 62.3% and 75.3%, respectively. After 32 days of post feeding, a significant increase in total haemocyte count (THC), different haemocyte count (DHC), respiratory bursts (RBs), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, phenoloxidase (PO) activity and transglutaminase (TG) activity, and meanwhile, a decreased haemolymph coagulation time was observed. Furthermore, phagocytic activity and clearance efficiency of prawns against Lactococcus garvieae infection were significantly increased. Prawns challenged with L. garvieae after 32 days of feeding at 1.0, 3.0 and 6.0 g kg(-1) had a significantly higher survival rate (33.3%, 40.0% and 56.7%) than those fed with the control diet. Subsequently, hypothermal (14 °C) stress was 43.4%, 50.0% and 50.0%, respectively. Altogether, we therefore recommend the dietary BPE administration at 6.0 g kg(-1) promotes growth, anti-hypothermal stress, and enhance immunity and resistance against L. garvieae in M. rosenbergii.
authors: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors: Wutti
authors: W
authors: Rattanavichai
authors: Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC. Electronic address: winton@mail.npust.edu.tw.
authors: Winton
authors: W
authors: Cheng
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003080:Cold Temperature; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D007113:Immunity, Innate; D017045:Lactococcus / Q000502:physiology*; D028521:Musa / Q000737:chemistry*; D033541:Palaemonidae / Q000254:growth & development / Q000276:immunology / Q000382:microbiology* / Q000502:physiology*; D010936:Plant Extracts
chemical_list: D010936:Plant Extracts
keywords: Anti-hypothermal stress; Banana peels extract; Disease resistance; Immunity; Macrobrachium rosenbergii