title: [Analysis of microdeletions of azoospermia factor genes on Y chromosome in infertile males].
abstract: OBJECTIVE
  To investigate the location and characteristics of microdeletions of Y chromosome azoospermia factor (AZF) genes in infertile males with azoospermia and severe oligozoospermia in southern Sichuan.
  
  
  METHODS
  Multiplex PCR was used to detect 18 sequence tagged sites (STS) involved in Y chromosome AZF microdeletions among 224 infertile males (including 134 azoospermia cases and 90 severe oligozoospermia cases) and 70 healthy males.
  
  
  RESULTS
  Among the 224 infertile males, the overall frequency of microdeletions was 12.1% (27/224), and were 13.4% (18/134) in those with azoospermia and 10.0% (9/90) in those with severe oligozoospermia. The most frequent microdeletions have occurred in the AZFc region (51.9%). Compared with the 6 STS loci recommended by European Academy of Andrology and European Molecular Genetics Quality Network, 22.7% more deletions were detected based on the 18 STS loci selected from the AZF region.
  
  
  CONCLUSIONS
  Identification of Y chromosome microdeletions has a significant implication on the diagnosis of male infertility. The most frequent microdeletions have occurred in the AZFc region in southern Sichuan. To use more sequence tagged sites for the screening can improve the reliability and detection rate of Y chromosome microdeletions.
authors: Department of Human Assisted Human Reproduction, the Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China. Email: 1987flpays@163.com.
authors: Li
authors: L
authors: Fu
authors: Xiguang
authors: X
authors: Mao
authors: Shaowei
authors: S
authors: Chen
authors: Hongbin
authors: H
authors: Zhang
authors: Mingyong
authors: M
authors: Wang
authors: Guiying
authors: G
authors: Huang
authors: Fang
authors: F
authors: Wang
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008875:Middle Aged