title: Renalase protects against contrast-induced nephropathy in Sprague-Dawley rats.
abstract: BACKGROUND
  Contrast-induced nephropathy (CIN) is the third leading cause of hospital-acquired acute renal failure. Oxidative stress, apoptosis and inflammation play crucial roles in CIN. Renalase is a newly discovered monoamine oxidase from the kidney. We hypothesize that renalase could protect against CIN through anti-oxidation, anti-inflammation and anti-apoptosis pathways.
  
  
  METHODS
  We tested our hypothesis in vivo with a rat model of Ioversol-induced CIN and in vitro. Sprague-Dawley rats were divided into 4 groups (n = 6 per group): control group, Ioversol group (rats subjected to Ioversol-induced CIN), Ioversol plus vehicle group (CIN rats pretreated with vehicle) and Ioversol plus renalase group (CIN rats pretreated with 2 mg/kg recombinant renalase). HK2 cells were treated with Ioversol or H2O2.
  
  
  RESULTS
  The results showed that pretreatment with renalase attenuated the deterioration of renal function, tubular necrosis, oxidative stress, apoptosis and inflammation (P<0.05). Furthermore, renalase protected HK2 cells against the cytotoxicity of Ioversol and suppressed Caspase-3 activity, oxidative stress and apoptosis induced by H2O2.
  
  
  CONCLUSIONS
  Recombinant renalase protected CIN in rats through anti-oxidation, anti-apoptosis and anti-inflammation mechanisms.
authors: Department of Radiology, Tongji University Affiliated Shanghai Tenth People's Hospital, Shanghai, China.
authors: Binghui
authors: B
authors: Zhao
authors: Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors: Qing
authors: Q
authors: Zhao
authors: Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors: Junhui
authors: J
authors: Li
authors: St. Vincent's Hospital, Melbourne, Australia.
authors: Tao
authors: T
authors: Xing
authors: Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors: Feng
authors: F
authors: Wang
authors: Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors: Niansong
authors: N
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003287:Contrast Media / Q000009:adverse effects*; D003404:Creatinine / Q000097:blood; D007249:Inflammation / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008995:Monoamine Oxidase / Q000494:pharmacology / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D014283:Triiodobenzoic Acids / Q000633:toxicity
chemical_list: D003287:Contrast Media; D020011:Protective Agents; D014283:Triiodobenzoic Acids; D003404:Creatinine; D008995:Monoamine Oxidase; C521557:renalase; C054871:ioversol
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