title: Mutagenic and genotoxic effects of Guelma's urban wastewater, Algeria.
abstract: Assessment of water pollution and its effect upon river biotic communities and human health is indispensable to develop control and management strategies. In this study, the mutagenicity and genotoxicity of urban wastewater of the city of Guelma in Algeria were examined between April 2012 and April 2013. For this, two biological tests, namely Amesand chromosomal aberrations (CA) test in Allium cepa root tips were employed on the samples collected from five different sampling stages (S1-S5). In Ames test, two strains of Salmonella typhimurium TA98 and TA100 with or without metabolic activation (S9-mix) were used. All water samples were found to be mutagenic to S. typhimurium TA98 with or without S9-mix. A significant decrease in mitotic index (MI) was observed with a decrease in the percentage of cells in the prophase and an increase in the telophase. Main aberrations observed were anaphase bridges, disturbed anaphase-telophase cells, vagrants and stickiness in anaphase-telophase cells. All treatments of wastewater in April 2012, at S5 in July 2012, at S1 and S5 in November 2012, at S5 in February 2013, and at S1 in April 2013 induced CA when compared to the negative control. Some physicochemical parameters and heavy metals (Cd, Pb, and Cu) were also recorded in the samples examined.
authors: Biology Department, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University 8 Mai 1945 Guelma, BP 401, Guelma, 24000, Algeria.
authors: Mouna
authors: M
authors: Tabet
authors: Ahlem
authors: A
authors: Abda
authors: Djamel E
authors: DE
authors: Benouareth
authors: Recep
authors: R
authors: Liman
authors: Muhsin
authors: M
authors: Konuk
authors: Messaouda
authors: M
authors: Khallef
authors: Ali
authors: A
authors: Taher
mesh_terms: D000462:Algeria; D000705:Anaphase; D002869:Chromosome Aberrations; D004249:DNA Damage; D004784:Environmental Monitoring; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000633:toxicity; D019697:Onions; D045483:Rivers; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development; D013692:Telophase; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D009153:Mutagens; D062065:Wastewater; D014874:Water Pollutants, Chemical
references: Mutat Res. 2004 Aug 18;552(1-2):141-51
references: 15288547
references: Mutat Res. 1998 Dec 3;420(1-3):37-48
references: 9838036
references: Mutat Res. 2004 Oct 4;554(1-2):375-85
references: 15450433
references: Sci Total Environ. 2005 Jul 15;347(1-3):46-52
references: 16084966
references: J Environ Radioact. 2003;68(3):235-48
references: 12782475
references: Mutat Res. 1999 May 19;426(2):183-8
references: 10350595
references: Environ Pollut. 1996;93(2):135-9
references: 15091352
references: Ecotoxicol Environ Saf. 2009 Oct;72(7):1925-32
references: 19524298
references: Ecotoxicol Environ Saf. 1991 Aug;22(1):94-125
references: 1915000
references: Chemosphere. 2013 Jun;92(1):1-9
references: 23557725
references: Mutat Res. 2000 Nov 20;455(1-2):29-60
references: 11113466
references: Ecotoxicol Environ Saf. 2010 Sep;73(6):1231-7
references: 20663557
references: Toxicology. 2005 Dec 15;216(2-3):244-52
references: 16168554
references: Mutat Res. 1996 Jul 5;368(3-4):171-9
references: 8692223
references: Hereditas. 1985;102(1):99-112
references: 3988545
references: Mutat Res. 1995 Apr;334(2):185-95
references: 7885371
references: Chemosphere. 2010 Aug;80(9):1056-61
references: 20605189
references: Mutat Res. 1998 Jun;410(3):223-36
references: 9630643
references: Chemosphere. 2009 May;75(7):934-8
references: 19201446
references: Chemosphere. 2003 May;51(6):539-43
references: 12615107
references: Ecotoxicol Environ Saf. 2010 Mar;73(3):320-5
references: 19910047
references: Mutat Res. 2009 Jul-Aug;682(1):71-81
references: 19577002
references: Ecotoxicol Environ Saf. 2009 Nov;72 (8):2090-5
references: 19647317
references: Mutagenesis. 2006 Jan;21(1):77-81
references: 16434449
references: Mutat Res. 1988 Feb;197(2):243-60
references: 3340086
references: Ecotoxicol Environ Saf. 2014 Mar;101:90-6
references: 24507132
references: Cytotechnology. 2015 Jan;67(1):157-63
references: 24337653
references: Chem Biol Interact. 2006 Mar 10;160(1):1-40
references: 16430879
references: Arch Environ Contam Toxicol. 1999 Feb;36(2):115-9
references: 9888954
references: J Hazard Mater. 2008 Jul 15;155(3):403-6
references: 18178309
references: Mutat Res. 1983 May;113(3-4):173-215
references: 6341825
references: Chemosphere. 2006 Oct;65(5):863-72
references: 16635506
references: Mutat Res. 2005 Jan 3;565(2):151-62
references: 15661613
references: J Prev Med Hyg. 2009 Sep;50(3):170-4
references: 20411651
references: Mutat Res. 2011 Nov 27;726(1):15-20
references: 21855648
references: Environ Pollut. 2006 Apr;140(3):506-15
references: 16168540
references: Mutat Res. 1998 Oct 12;418(2-3):113-9
references: 9757013
references: Environ Monit Assess. 2011 Dec;183(1-4):517-24
references: 21365443
references: Sci Total Environ. 2010 Feb 1;408(5):1228-33
references: 20018345