title: Laser-induced shockwave paired with FRET: a method to study cell signaling.
abstract: Cells within the body are subject to various forces; however, the details concerning the way in which cells respond to mechanical stimuli are not well understood. We demonstrate that laser-induced shockwaves (LIS) combined with biosensors based on fluorescence resonance energy transfer (FRET) is a promising new approach to study biological processes in single live cells. As "proof-of-concept," using a FRET biosensor, we show that in response to LIS, cells release intracellular calcium. With the parameters used, cells retain their morphology and remain viable. LIS combined with FRET permits observation of the cells immediate response to a sudden shear force.
authors: Institute of Engineering in Medicine, University of California, San Diego 9500, Gilman Drive La Jolla, California, 92093-0435.
authors: Veronica
authors: V
authors: Gomez-Godinez
authors: Daryl
authors: D
authors: Preece
authors: Linda
authors: L
authors: Shi
authors: Nima
authors: N
authors: Khatibzadeh
authors: Derrick
authors: D
authors: Rosales
authors: Yijia
authors: Y
authors: Pan
authors: Lie
authors: L
authors: Lei
authors: Yingxiao
authors: Y
authors: Wang
authors: Michael W
authors: MW
authors: Berns
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D042783:Endothelial Cells / Q000502:physiology* / Q000528:radiation effects*; D031541:Fluorescence Resonance Energy Transfer; D007834:Lasers; D055595:Mechanical Phenomena; D061848:Optical Imaging / Q000379:methods; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D002118:Calcium
keywords: Calcium; FRET; laser-induced shockwave
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