title: Detection and quantitation of low abundance oligosaccharides in recombinant monoclonal antibodies.
abstract: Oligosaccharides are critical for structural integrity, stability, and biological functions of recombinant monoclonal antibodies. It is relatively easy to characterize, quantify, and determine the impact of major glycoforms. While challenging to detect and quantify, certain low abundance oligosaccharides are highly relevant to the stability and functions of recombinant monoclonal antibodies. Methods were established in this study based on enzymatic digestion to consolidate peaks of the same type of oligosaccharides by removing heterogeneity and thus increase detectability of low abundance peaks. Endo H was used to collapse high mannose oligosaccharides to a single peak of GlcNAc for ease of detection and quantitation. β-Galactosidase and β-N-acetylhexosaminidase were used to convert complex oligosaccharides into two peaks containing either GlcNAc2Man3Fuc or GlcNAc2Man3, which simplified the chromatograms and data analysis. More importantly, low abundance hybrid oligosaccharides can only be detected and qualified after β-galactosidase and β-N-acetylhexosaminidase digestion. Detection and quantitation of low abundance oligosaccharides can also be achieved using a combination of all three enzymes. These methods can be applied to the development of recombinant monoclonal antibody therapeutics.
authors: Product Characterization, Alexion Pharmaceuticals Inc. , 352 Knotter Drive, Cheshire, Connecticut 06410, United States.
authors: Gomathinayagam
authors: G
authors: Ponniah
authors: Christine
authors: C
authors: Nowak
authors: Nidia
authors: N
authors: Gonzalez
authors: Dino
authors: D
authors: Miano
authors: Hongcheng
authors: H
authors: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000737:chemistry; D006801:Humans; D008358:Mannose / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D009844:Oligosaccharides; D011994:Recombinant Proteins; D005643:Fucose; D001616:beta-Galactosidase; D008358:Mannose