title: Type III secretion system and virulence markers highlight similarities and differences between human- and plant-associated pseudomonads related to Pseudomonas fluorescens and P. putida.
abstract: Pseudomonas fluorescens is commonly considered a saprophytic rhizobacterium devoid of pathogenic potential. Nevertheless, the recurrent isolation of strains from clinical human cases could indicate the emergence of novel strains originating from the rhizosphere reservoir, which could be particularly resistant to the immune system and clinical treatment. The importance of type three secretion systems (T3SSs) in the related Pseudomonas aeruginosa nosocomial species and the occurrence of this secretion system in plant-associated P. fluorescens raise the question of whether clinical isolates may also harbor T3SSs. In this study, isolates associated with clinical infections and identified in hospitals as belonging to P. fluorescens were compared with fluorescent pseudomonads harboring T3SSs isolated from plants. Bacterial isolates were tested for (i) their genetic relationships based on their 16S rRNA phylogeny, (ii) the presence of T3SS genes by PCR, and (iii) their infectious potential on animals and plants under environmental or physiological temperature conditions. Two groups of bacteria were delineated among the clinical isolates. The first group encompassed thermotolerant (41°C) isolates from patients suffering from blood infections; these isolates were finally found to not belong to P. fluorescens but were closely related and harbored highly conserved T3SS genes belonging to the Ysc-T3SS family, like the T3SSs from P. aeruginosa. The second group encompassed isolates from patients suffering from cystic fibrosis; these isolates belonged to P. fluorescens and harbored T3SS genes belonging to the Hrp1-T3SS family found commonly in plant-associated P. fluorescens.
authors: INRA, UMR 1347 Agroécologie, Dijon, France.
authors: Sylvie
authors: S
authors: Mazurier
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Annabelle
authors: A
authors: Merieau
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Dorian
authors: D
authors: Bergeau
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Victorien
authors: V
authors: Decoin
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Daniel
authors: D
authors: Sperandio
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Alexandre
authors: A
authors: Crépin
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France.
authors: Corinne
authors: C
authors: Barbey
authors: Université de Franche-Comté, Laboratoire de Bacteriologie, Faculté de médecine, Besançon, France.
authors: Katy
authors: K
authors: Jeannot
authors: Normandie Université, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale-EA 4358-VASI-PRIMACEN (IRIB), Université de Rouen, Mont-Saint-Aignan, France.
authors: Maïté
authors: M
authors: Vicré-Gibouin
authors: Université de Franche-Comté, Laboratoire de Bacteriologie, Faculté de médecine, Besançon, France.
authors: Patrick
authors: P
authors: Plésiat
authors: INRA, UMR 1347 Agroécologie, Dijon, France philippe.lemanceau@dijon.inra.fr xavier.latour@univ-rouen.fr.
authors: Philippe
authors: P
authors: Lemanceau
authors: Normandie Université, Laboratoire de Microbiologie, Signaux et Microenvironnement-EA 4312 & IRIB Université de Rouen-IUT Evreux, Evreux, France philippe.lemanceau@dijon.inra.fr xavier.latour@univ-rouen.fr.
authors: Xavier
authors: X
authors: Latour
mesh_terms: D016470:Bacteremia / Q000382:microbiology; D058947:Bacterial Secretion Systems / Q000235:genetics*; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004023:Dictyostelium / Q000254:growth & development / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012141:Respiratory Tract Infections / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013696:Temperature; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D058947:Bacterial Secretion Systems; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D037521:Virulence Factors
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