title: Enzymatic cleavage of cell surface proteins of pig and cow erythrocytes and its effect on concanavalin-mediated agglutinability.
abstract: Study was carried out to understand and compare architecture of the proteins of erythrocyte cell surface of some mammals viz., Homo sapiens (human), Sus scorfa domestica (pig) and Bos taurus domestica (cow). In this study, we investigated the action of proteinases viz., trypsin and chymotrypsin and neuraminidase on the erythrocyte surface proteins and erythrocyte agglutination tendency with a lectin (concanavalin A). The electrophoretic pattern of membrane proteins and glycophorins (analyzed by SDS-PAGE and visualized by Coomassie brilliant blue and periodic acid-schiff stains, respectively) and concanavalin A (Con A) agglutinability revealed that: (i) There were variations in the number and molecular weights of glycophorins in human, pig and cow, (ii) trypsin action on pig and cow erythrocyte membrane proteins was similar, unlike human, (iii) glycophorins degradation by trypsin and chymotrypsin was not similar in pig, as compared to that of human and cow, (iv) erythrocytes agglutination with Con A was significantly different due to differences in membrane composition and alterations in the surface proteins after enzyme treatment, (v) a direct correlation was found between degradation of glycophorins and Con A agglutinability, and (vi) removal of erythrocyte surface sialic acids by neuraminidase specifically indicated an increase in Con A agglutinability of pig and cow erythrocytes, similar to human.
authors: Savita
authors: S
authors: Sharma
authors: Sadashiv M
authors: SM
authors: Gokhale
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism*; D006384:Hemagglutination / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000494:pharmacology*; D013045:Species Specificity; D013552:Swine
chemical_list: D008565:Membrane Proteins; D003208:Concanavalin A; D010447:Peptide Hydrolases