title: Angiogenesis effect of therapeutic ultrasound on ischemic hind limb in mice.
abstract: Although significant progress in bypass surgery and catheter intervention against peripheral artery disease, the number of severe critical limb ischemia (CLI) patients is increasing. Thus, it is crucial to develop new, non-invasive therapeutic strategies. The purpose of this study was to determine the mechanism of therapeutic ultrasound (TUS) on ischemic angiogenesis using mouse model of hindlimb ischemia and the cellular/molecular mechanisms underlying TUS-related neovascularization. The hindlimb ischemic mice were exposed to extracorporeal TUS for 3, 6, 9 minute per day (1 MHz, 0.3 W/cm(2)) until day 14 after left femoral artery ligation. Increased blood perfusion and capillary density were determined following 9 min of TUS compared with ischemic group. Moreover, TUS treatment increased the protein levels of vascular endothelial growth factor (VEGF), hypoxic inducible factor-1α (HIF-1α), endothelial nitric oxide synthase (eNOS) and p-Akt in vivo. TUS promoted capillary-like tube formation, migration and motility of human umbilical venous endothelial cells (HUVECs). Furthermore, the protein expressions of VEGF, eNOS and p-Akt were increased after TUS treatment. In conclusion, TUS therapy promotes postnatal neovascularization through multiple angiogenic pathways in mice model of ischemic hindlimb.
authors: Department of Gerontology, Xin Hua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Jing-Juan
authors: JJ
authors: Huang
authors: Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of Medicine Dongfang Road 1630, Shanghai 200127, China.
authors: Yi-Qin
authors: YQ
authors: Shi
authors: Department of Gerontology, Xin Hua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Rui-Lin
authors: RL
authors: Li
authors: Department of Otolaryngology, Gong li Hospital Miaopu Road 219, Shanghai 200135, China.
authors: An
authors: A
authors: Hu
authors: Shanghai Acoustics Laboratory, Chinese Academy of Science Xiaonuqiao Road 456, Shanghai 200032, China.
authors: Hong-Sheng
authors: HS
authors: Zhou
authors: Institute of Acoustics, Tongji University Siping Road 1239, Shanghai 200092, China.
authors: Qian
authors: Q
authors: Cheng
authors: Institute of Acoustics, Tongji University Siping Road 1239, Shanghai 200092, China.
authors: Zheng
authors: Z
authors: Xu
authors: Department of Cardiology, The Second Hospital of Shanxi Medical University Wuyi Road 382, Taiyuan 030001, China.
authors: Zhi-Ming
authors: ZM
authors: Yang
authors: Department of Vascular Surgery, Ren Ji Hospital, Shanghai Jiaotong University School of Medicine Dongfang Road 1630, Shanghai 200127, China.
authors: Chang-Ning
authors: CN
authors: Hao
authors: Department of Gerontology, Xin Hua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Jun-Li
authors: JL
authors: Duan
keywords: Therapeutic ultrasound; VEGF; angiogenesis; critical limb ischemia
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