title: Detection of Bacterial Biofilms in Chronic Pharyngitis Resistant to Medical Treatment.
abstract: OBJECTIVE
  To evaluate the role played by adenoids as a reservoir for infection in children assigned for adenoidectomy.
  
  
  METHODS
  The study included 35 children with adenoid hypertrophy. All patients underwent clinical examination and adenoidectomy, adenotonsillectomy, or myringotomy with insertion of aeration tube according to indications. Surgical specimens were processed for conventional bacterial culture examination and to assay for biofilm formation. The obtained adherence values using spectrophotometer at 595 nm (OD595) was used to classify isolates according to its biofilm forming capacity.
  
  
  RESULTS
  We did adenotonsillectomy and myringotomy with insertion of aeration tube in 5 patients having adenotonsillitis with otitis media with effusion. We did adenotonsillectomy in 12 patients having adenotonsillitis and adenoidectomy in 18 patients having adenoid hypertrophy. Thirty-one surgical specimens showed bacterial growth on conventional media, while 4 specimens failed to give growth. The predominant organism was H influenzae then Staph aureus and Strept pneumoniae. Thirty-two specimens showed biofilm forming capacity (BFC) of variable extent, while others showed no BFC.
  
  
  CONCLUSIONS
  Adenoids act as a bacterial reservoir secondary to bacterial biofilm formation so could induce chronicity and initiate development of complications. Determination of BFC using the proposed protocol is feasible, inexpensive, and available and spares the need for sophisticated instruments or approaches.
authors: Department of Otorhinolaryngology, Faculty of Medicine, Cairo University, Egypt Hatembadran@hotmail.com.
authors: Hatem
authors: H
authors: Badran
authors: Department of Otorhinolaryngology, Faculty of Medicine, Zagazig University, Egypt.
authors: Mohamad
authors: M
authors: Salah
authors: Department of Otorhinolaryngology, Faculty of Medicine, Fayoum University, Egypt.
authors: Mohamed
authors: M
authors: Fawzy
authors: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Egypt.
authors: Amal
authors: A
authors: Sayed
authors: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Egypt.
authors: Doaa
authors: D
authors: Ghaith
mesh_terms: D000233:Adenoidectomy; D000234:Adenoids / Q000382:microbiology* / Q000601:surgery / Q000648:ultrastructure; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001419:Bacteria; D018441:Biofilms; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D024881:Drug Resistance, Bacterial; D005240:Feasibility Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010612:Pharyngitis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D012189:Retrospective Studies; D013053:Spectrophotometry; D046509:Tissue Culture Techniques
chemical_list: D000900:Anti-Bacterial Agents
keywords: H influenzae; adenoid; bacterial growth; biofilm forming capacity; otitis media