title: Composition and antimicrobial activity of the essential oils of Laserpitium latifolium L. and L. ochridanum Micevski (Apiaceae).
abstract: The chemical composition and antimicrobial activity of essential oils of Laserpitium latifolium and L. ochridanum were investigated. The essential oils were isolated by steam distillation and characterized by GC-FID and GC/MS analyses. All essential oils were distinguished by high contents of monoterpenes, and α-pinene was the most abundant compound in the essential oils of L. latifolium underground parts and fruits (contents of 44.4 and 44.0%, resp.). The fruit essential oil was also rich in sabinene (26.8%). Regarding the L. ochridanum essential oils, the main constituents were limonene in the fruit oil (57.7%) and sabinene in the herb oil (25.9%). The antimicrobial activity of these essential oils as well as that of L. ochridanum underground parts, whose composition was reported previously, was tested by the broth-microdilution method against four Gram-positive and three Gram-negative bacteria and two Candida albicans strains. Except the L. latifolium underground-parts essential oil, the other investigated oils showed a high antimicrobial potential against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, or Candida albicans (minimal inhibitory concentrations of 13.0-73.0 μg/ml), comparable to or even higher than that of thymol, which was used as reference compound.
authors: University of Belgrade, Faculty of Pharmacy, Department of Pharmacognosy, V. Stepe 450, RS-11221 Belgrade (phone: +381-11-3951319; fax: +381-11-3972840); Ghent University, Faculty of Science, Laboratory for Organic and Bio-organic Synthesis, Department of Organic and Macromolecular Chemistry, Krijgslaan 281 S4, BE-9000 Ghent, (phone: +32-9-2644476; fax: +32-9-2644998). Visnja.Popovic@UGent.be, visnjap@pharmacy.bg.ac.rs.
authors: Višnja B
authors: VB
authors: Popović
authors: Silvana D
authors: SD
authors: Petrović
authors: Marina T
authors: MT
authors: Milenković
authors: Milica M
authors: MM
authors: Drobac
authors: Maria A
authors: MA
authors: Couladis
authors: Marjan S
authors: MS
authors: Niketić
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile
keywords: Antimicrobial activity; Essential oils; Laserpitium species