title: Serological Profile of HSV-2 in STD Patients: Evaluation of Diagnostic Utility of HSV-2 IgM and IgG Detection.
abstract: OBJECTIVE
  The present study was undertaken to determine Herpes Simplex Virus-2 seroprevalence in sexually active adults aged 20-49 and to investigate the correlation with sociodemographic characteristics and to find its association with other sexually transmitted diseases especially HIV and also to assess the proportion of primary and reactivated HSV-2 cases.
  
  
  METHODS
  This prospective study was carried out for a period of six months in a tertiary care hospital. Serum samples were taken from 91 patients attending the out Patient clinic of the Department of Venereology. The serological testing for HSV-2 was performed on all the specimens by using Euroimmun anti-HSV2 (gG2) IgM ELISA and IgG ELISA.
  
  
  RESULTS
  Out of the 91 STD patients in the study group, 18 males (34.62%) and 14 females (36.84%) tested positive for HSV-2 antibodies. Seropositivity rate is 35.16%. More number of HSV-2 positive cases were seen among males, older age, rural residence, low socioeconomic status, single marital status, irregular condom usage during the sexual intercourses with new partners and with higher number of sexual partners during lifetime. HSV-2 IgM alone was positive in three cases, HSV-2 IgG alone was positive in 26 cases and three had a positive HSV-2 IgM and IgG result. Addition of IgM testing increased rate of detecting seroconversion, 31.87%, when only IgG ELISA was used, to 35.16 % patients when IgM test was added. In the study group four cases tested positive for VDRL, and one of them was a known positive case. Among the 55 HIV positive cases in the study group, HSV 2 was positive in 17 cases and among the 36 HIV negative cases HSV 2 was positive in 15 cases. (30.91% and 47.22%).Though the number of HIV cases were high, HSV 2 positivity among them was statistically not significant.
  
  
  CONCLUSIONS
  The purpose of screening for HSV-2 is not only to identify seropositivity, but to help seropositive people identify symptoms and protect themselves from acquiring HIV and to protect their partners and seronegative people from acquiring HSV-2 and/or HIV.
authors: Senior Assistant Professor, Department of Microbiology, Tirunelveli Medical College , Tirunelveli, Tamil Nadu, India .
authors: V P
authors: VP
authors: Amudha
authors: Student, Department of Microbiology, Tirunelveli Medical College , Tirunelveli, Tamil Nadu, India .
authors: Rashetha
authors: Senior Assistant Professor, Department of Microbiology, Tirunelveli Medical College , Tirunelveli, Tamil Nadu, India .
authors: G
authors: G
authors: Sucilathangam
authors: Senior Assistant Professor, Department of Microbiology, Tirunelveli Medical College , Tirunelveli, Tamil Nadu, India .
authors: B
authors: B
authors: Cinthujah
authors: Professor and Head, Department of Microbiology, Tirunelveli Medical College , Tirunelveli, Tamil Nadu, India .
authors: C
authors: C
authors: Revathy
keywords: HIV; Herpes simplex virus-2; IgG ELISA; IgM ELISA; Seroprevalence
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