title: Multicentric study underlining the interest of adding CD5, CD7 and CD56 expression assessment to the flow cytometric Ogata score in myelodysplastic syndromes and myelodysplastic/myeloproliferative neoplasms.
abstract: Although numerous recent publications have demonstrated interest in multiparameter flow cytometry in the investigation of myelodysplastic disorders, it is perceived by many laboratory hematologists as difficult and expensive, requiring a high level of expertise. We report a multicentric open real-life study aimed at evaluating the added value of the technically simple flow cytometry score described by the Ogata group for the diagnosis of myelodysplastic syndromes. A total of 652 patients were recruited prospectively in four different centers: 346 myelodysplastic syndromes, 53 myelodysplastic/myeloproliferative neoplasms, and 253 controls. The Ogata score was assessed using CD45 and CD34 staining, with the addition of CD10 and CD19. Moreover, labeling of CD5, CD7 and CD56 for the evaluation of myeloid progenitors and monocytes was tested on a subset of 294 patients. On the whole series, the specificity of Ogata score reached 89%. Respective sensitivities were 54% for low-risk myelodysplastic syndromes, 68% and 84% for type 1 and type 2 refractory anemia with excess of blasts, and 72% for myelodysplastic/myeloproliferative neoplasms. CD5 expression was poorly informative. When adding CD56 or CD7 labeling to the Ogata score, sensitivity rose to 66% for low-risk myelodysplastic syndromes, to 89% for myelodysplastic/myeloproliferative neoplasms and to 97% for refractory anemia with excess of blasts. This large multicenter study confirms the feasibility of Ogata scoring in routine flow cytometry diagnosis but highlights its poor sensitivity in low-risk myelodysplastic syndromes. The addition of CD7 and CD56 in flow cytometry panels improves the sensitivity but more sophisticated panels would be more informative.
authors: Service d'Hématologie Biologique, Hôpitaux Universitaires Paris Centre-Cochin, Faculté de Médecine Paris Descartes, INSERM U1016, UMR 8104, Paris.
authors: Valérie
authors: V
authors: Bardet
authors: Département d'Hématologie et d'Immunologie Biologiques, Hôpitaux Universitaires Henri Mondor, APHP, Faculté de Médecine UPEC, Créteil.
authors: Orianne
authors: O
authors: Wagner-Ballon
authors: Service d'Hématologie Biologique, CHU de Dijon.
authors: Julien
authors: J
authors: Guy
authors: Service d'Hématologie Biologique, Hôpital Dupuytren, CHU de Limoges, Faculté de Médecine et UMR CNRS 7276, Limoges.
authors: Céline
authors: C
authors: Morvan
authors: Service d'Hématologie Biologique, Hôpitaux Universitaires Paris Centre-Cochin, Faculté de Médecine Paris Descartes, INSERM U1016, UMR 8104, Paris.
authors: Camille
authors: C
authors: Debord
authors: Service d'Hématologie Biologique, Hôpital Dupuytren, CHU de Limoges, Faculté de Médecine et UMR CNRS 7276, Limoges.
authors: Franck
authors: F
authors: Trimoreau
authors: Département d'Hématologie et d'Immunologie Biologiques, Hôpitaux Universitaires Henri Mondor, APHP, Faculté de Médecine UPEC, Créteil.
authors: Emmanuel
authors: E
authors: Benayoun
authors: Service d'Hématologie Biologique, Hôpitaux Universitaires Paris Centre-Cochin, Faculté de Médecine Paris Descartes, INSERM U1016, UMR 8104, Paris.
authors: Nicolas
authors: N
authors: Chapuis
authors: Département d'Hématologie et d'Immunologie Biologiques, Hôpitaux Universitaires Henri Mondor, APHP, Faculté de Médecine UPEC, Créteil.
authors: Nicolas
authors: N
authors: Freynet
authors: Service d'Hématologie Biologique, CHU de Dijon.
authors: Cédric
authors: C
authors: Rossi
authors: Service d'Hématologie Biologique, Hôpitaux Universitaires Paris Centre-Cochin, Faculté de Médecine Paris Descartes, INSERM U1016, UMR 8104, Paris.
authors: Stéphanie
authors: S
authors: Mathis
authors: Service d'Hématologie Clinique, Hôpital Dupuytren, CHU de Limoges.
authors: Marie-Pierre
authors: MP
authors: Gourin
authors: Service d'Hématologie Clinique, Hôpitaux Universitaires Henri Mondor, APHP, Créteil.
authors: Andréa
authors: A
authors: Toma
authors: Service d'Hématologie Biologique, CHU de Nantes, France.
authors: Marie C
authors: MC
authors: Béné
authors: Service d'Hématologie Biologique, Hôpital Dupuytren, CHU de Limoges, Faculté de Médecine et UMR CNRS 7276, Limoges.
authors: Jean
authors: J
authors: Feuillard
authors: Service d'Hématologie Biologique, Hôpital Dupuytren, CHU de Limoges, Faculté de Médecine et UMR CNRS 7276, Limoges estelle.guerin@chu-limoges.fr.
authors: Estelle
authors: E
authors: Guérin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019011:Antigens, CD7 / Q000235:genetics / Q000378:metabolism*; D018956:CD5 Antigens / Q000235:genetics / Q000378:metabolism*; D019002:CD56 Antigen / Q000235:genetics / Q000378:metabolism*; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D054437:Myelodysplastic-Myeloproliferative Diseases / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D019011:Antigens, CD7; D018956:CD5 Antigens; D019002:CD56 Antigen
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