title: Comparative proteomics analysis reveals the mechanism of fertility alternation of thermosensitive genic male sterile rice lines under low temperature inducement.
abstract: Thermosensitive genic male sterile (TGMS) rice line has made great economical contributions in rice production. However, the fertility of TGMS rice line during hybrid seed production is frequently influenced by low temperature, thus leading to its fertility/sterility alteration and hybrid seed production failure. To understand the mechanism of fertility alternation under low temperature inducement, the extracted proteins from young panicles of two TGMS rice lines at the fertility alternation sensitivity stage were analyzed by 2DE. Eighty-three protein spots were found to be significantly changed in abundance, and identified by MALDI-TOF-TOF MS. The identified proteins were involved in 16 metabolic pathways and cellular processes. The young panicles of TGMS rice line Zhu 1S possessed the lower ROS-scavenging, indole-3-acetic acid level, soluble protein, and sugar contents as well as the faster anther wall disintegration than those of TGMS rice line Zhun S. All these major differences might result in that the former is more stable in fertility than the latter. Based on the majority of the 83 identified proteins, together with microstructural, physiological, and biochemical results, a possible fertile alteration mechanism in the young panicles of TGMS rice line under low temperature inducement was proposed. Such a result will help us in breeding TGMS rice lines and production of hybrid seed.
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, P. R. China.
authors: Liru
authors: L
authors: Song
authors: College of Agronomy, Hunan Agricultural University, Collaborative Innovation Center of Grain and Oil crops in South China, Changsha, P. R. China.|State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, P. R. China.
authors: Zhongqi
authors: Z
authors: Liu
authors: Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, P. R. China.
authors: Jianhua
authors: J
authors: Tong
authors: Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, P. R. China.
authors: Langtao
authors: L
authors: Xiao
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, P. R. China.
authors: Hao
authors: H
authors: Ma
authors: College of Agronomy, Hunan Agricultural University, Collaborative Innovation Center of Grain and Oil crops in South China, Changsha, P. R. China.|State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, P. R. China.
authors: Haiqing
authors: H
authors: Zhang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000502:physiology / Q000648:ultrastructure; D011392:Proline / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012098:Reproduction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D004798:Enzymes; D005875:Gibberellins; D007210:Indoleacetic Acids; D010940:Plant Proteins; C030737:indoleacetic acid; D000040:Abscisic Acid; D011392:Proline
keywords: Fertility alternation mechanism; Low temperature inducement; Plant proteomics; Thermosensitive genic male sterile rice