title: Utilising the resources of the International Knockout Mouse Consortium: the Australian experience.
abstract: Mouse models play a key role in the understanding gene function, human development and disease. In 2007, the Australian Government provided funding to establish the Monash University embryonic stem cell-to-mouse (ES2M) facility. This was part of the broader Australian Phenomics Network, a national infrastructure initiative aimed at maximising access to global resources for understanding gene function in the mouse. The remit of the ES2M facility is to provide subsidised access for Australian biomedical researchers to the ES cell resources available from the International Knockout Mouse Consortium (IKMC). The stated aim of the IKMC is to generate a genetically modified mouse ES cell line for all of the ~23,000 genes in the mouse genome. The principal function of the Monash University ES2M service is to import genetically modified ES cells into Australia and to convert them into live mice with the potential to study human disease. Through advantages of economy of scale and established relationships with ES cell repositories worldwide, we have created over 110 germline mouse strains sourced from all of the major ES providers worldwide. We comment on our experience in generating these mouse lines; providing a snapshot of a "clients" perspective of using the IKMC resource and one which we hope will serve as a guide to other institutions or organisations contemplating establishing a similar centralised service.
authors: Australian Phenomics Network (APN), Embryonic Stem Cell (ES Cell)-to-Mouse Service, Monash University, Clayton, VIC, 3800, Australia.
authors: Leanne M
authors: LM
authors: Cotton
authors: Michelle L
authors: ML
authors: Meilak
authors: Tanya
authors: T
authors: Templeton
authors: Jose G
authors: JG
authors: Gonzales
authors: Arianna
authors: A
authors: Nenci
authors: Melissa
authors: M
authors: Cooney
authors: Dirk
authors: D
authors: Truman
authors: Fleur
authors: F
authors: Rodda
authors: Alyce
authors: A
authors: Lynas
authors: Elizabeth
authors: E
authors: Viney
authors: Nadia
authors: N
authors: Rosenthal
authors: Deborah M
authors: DM
authors: Bianco
authors: Moira K
authors: MK
authors: O'Bryan
authors: Ian M
authors: IM
authors: Smyth
mesh_terms: D000818:Animals; D001315:Australia; D035843:Biomedical Research / Q000458:organization & administration; D002460:Cell Line; D053595:Embryonic Stem Cells; D051379:Mice; D018345:Mice, Knockout
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