title: In vitro mechanistic study of endosulfan-induced spermatogenic cell apoptosis in the mouse.
abstract: To investigate the mechanisms of endosulfan-induced reproductive toxicity, the spermatogenic cell lines (GC-1 spg) of mice were treated with 0, 6, 12, and 24 μg/ml endosulfan for 24 h in vitro The results showed that endosulfan induced apoptosis as well as oxidative stress and mitochondrial dysfunction. Reactive oxygen species and damage of mitochondrial structure were considered as major factors to GC-1 spg cells apoptosis. We further examined the expression of apoptosis-related proteins in mitochondria pathway by Western blot and immunohistochemistry analysis as well as activities. The results showed that endosulfan significantly improved the expressions of cytochrome c and B-cell lymphoma 2 (Bcl-2)-associated X protein and increased the activities of caspases 9 and 3 as well as the downregulation of the expression of Bcl-2 in GC-1 spg cells. The results suggested that exposure to endosulfan might induce the apoptosis of spermatogenic cells via mitochondria-dependent pathway mediated by oxidative stress resulting in the damage of mitochondrial structure and mitochondrial dysfunction.
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors: Ying
authors: Y
authors: Xu
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors: Na
authors: N
authors: Wang
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors: Zhi-Xiong
authors: ZX
authors: Shi
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors: Yan-Bo
authors: YB
authors: Li
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China xqzhou@ccmu.edu.cn.
authors: Xian-Qing
authors: XQ
authors: Zhou
authors: Department of Health Toxicology and Health Chemistry, School of Public Health, Capital Medical University, Beijing, People's Republic of China Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors: Zhi-Wei
authors: ZW
authors: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D053453:Caspase 9 / Q000737:chemistry / Q000378:metabolism; D002461:Cell Line, Transformed; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004726:Endosulfan / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000819:agonists / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051028:bcl-2-Associated X Protein / Q000819:agonists / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D004785:Environmental Pollutants; D007306:Insecticides; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; D045304:Cytochromes c; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9; D004726:Endosulfan
keywords: Endosulfan; apoptosis; mechanism; spermatogenic cells