title: Dynamic subcellular localization of aquaporin-7 in white adipocytes.
abstract: Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.
authors: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. Electronic address: miyauchi@z3.keio.jp.
authors: Takayuki
authors: T
authors: Miyauchi
authors: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
authors: Hiroyuki
authors: H
authors: Yamamoto
authors: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
authors: Yoichiro
authors: Y
authors: Abe
authors: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
authors: Go J
authors: GJ
authors: Yoshida
authors: Department of Health Science and Technology, Aalborg University, Fredrik Bajersvej 7, 9220 Aalborg, Denmark.
authors: Aleksandra
authors: A
authors: Rojek
authors: Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Tokyo 113-8519, Japan.
authors: Eisei
authors: E
authors: Sohara
authors: Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Tokyo 113-8519, Japan.
authors: Shinichi
authors: S
authors: Uchida
authors: Department of Health Science and Technology, Aalborg University, Fredrik Bajersvej 7, 9220 Aalborg, Denmark.
authors: Søren
authors: S
authors: Nielsen
authors: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. Electronic address: myasui@a3.keio.jp.
authors: Masato
authors: M
authors: Yasui
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D041721:3T3-L1 Cells; D052438:Adipocytes, White / Q000378:metabolism*; D000818:Animals; D020346:Aquaporins / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D021381:Protein Transport
chemical_list: D020346:Aquaporins; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; C492986:Abhd5 protein, mouse; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: Adipocyte; Aquaporin-7; Comparative gene identification 58; Lipolysis