title: Cortical neuronal loss after chronic prenatal hypoxia: a comparative laboratory study.
abstract: OBJECTIVE
  The purpose of this study was to investigate the prenatal hypoxic effect on the fetal brain development.
  
  
  METHODS
  We used the guinea pig chronic placental insufficiency model to investigate the effect of hypoxia on fetal brain development. We ligated unilateral uterine artery at 30-32 days of gestation (dg : with term defined as -67 dg). At 50 dg, 60 dg, fetuses were sacrificed and assigned to either the growth-restricted (GR) or control (no ligation) group. After fixation, dissection, and sectioning of cerebral tissue from these animals, immunohistochemistry was performed with NeuN antibody, which is a mature neuronal marker in the cerebral cortex.
  
  
  RESULTS
  The number of NeuN-immunoreactive (IR) cells in the cerebral cortex did not differ between the GR and control groups at 50 dg. However, the number of NeuN-IR cells was lesser in GR fetuses than in controls at 60 dg (p<0.05).
  
  
  CONCLUSIONS
  These findings show that chronic prenatal hypoxia affect the number of neuron in the cerebral cortex of guinea pig fetus at 60 dg. The approach used in this study is helpful for extending our understanding of neurogenesis in the cerebral cortex, and the findings may be useful for elucidating the brain injury caused by prenatal hypoxia.
authors: Department of Anatomy, School of Medicine, Chosun University, Gwangju, Korea.
authors: Yoon Young
authors: YY
authors: Chung
authors: Department of Anatomy, School of Medicine, Chosun University, Gwangju, Korea.
authors: Yong Hyun
authors: YH
authors: Jeon
authors: Department of Neurosurgery, School of Medicine, Chosun University, Gwangju, Korea.
authors: Seok Won
authors: SW
authors: Kim
keywords: Cerebral cortex; Hypoxia; Neuron
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