title: Ethanol extract of Portulaca Oleracea L. reduced the carbon tetrachloride induced liver injury in mice involving enhancement of NF-κB activity.
abstract: Acute hepatic injury causes high morbidity and mortality world-wide. Management of severe acute hepatic failure continues to be one of the most challenging problems in clinical medicine. In present study, carbon tetrachloride (CCl4) was used to induce acute liver damage in mice and the protective effects of ethanol extract of Portulaca Oleracea L. (PO) were examined. The aminotransferase activities were biochemical estimated and the liver damage was tested by morphological histological analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. The role of PO on the activity of NF-κB was determined by luciferase reporter gene assay and immunohistochemistry. The level of p-p65 was tested by western blot. Our results showed that PO administration on mice would decrease the serum aminotransferase level and reduced the liver histological damage. We also found that nuclear translocation of p65 was enhanced in liver tissues of mice treated with PO compared with control animals. In addition, in cultured hepatic cells, PO increased the NF-κB luciferase reporter gene activity and upregulated the level of phosphorylation of p65, but had no effects on mice liver SOD activity and MDA level. Collectively, PO attenuated CCl4 induced mice liver damage by enhancement of NF-κB activity.
authors: Division of Nephrology, Kidney Institute of PLA, Changzheng Hospital Shanghai, China ; The 401 Hospital of PLA Qingdao, Shandong, China.
authors: Hongguang
authors: H
authors: Shi
authors: Department of Emergency of Changzheng Hospital, The Second Military Medical University Shanghai, China.
authors: Xuefeng
authors: X
authors: Liu
authors: Department of Haematology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors: Gusheng
authors: G
authors: Tang
authors: The 401 Hospital of PLA Qingdao, Shandong, China.
authors: Haiyan
authors: H
authors: Liu
authors: Division of Nephrology, Kidney Institute of PLA, Changzheng Hospital Shanghai, China.
authors: Yinghui
authors: Y
authors: Zhang
authors: Division of Nephrology, Kidney Institute of PLA, Changzheng Hospital Shanghai, China.
authors: Bo
authors: B
authors: Zhang
authors: Division of Nephrology, Kidney Institute of PLA, Changzheng Hospital Shanghai, China.
authors: Xuezhi
authors: X
authors: Zhao
authors: Department of Military Hygiene, Faculty of Naval Medicine, The Second Military Medical University 800 Xiangyin Road, Shanghai, China.
authors: Wanyin
authors: W
authors: Wang
keywords: Carbon tetrachloride (CCl4); NF-κB; ethanol extract of Portulaca Oleracea L (PO); liver injury
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