title: [Expressions of osteogenesis related factors in mg63 cells co-cultured with domestic porous tantalum].
abstract: OBJECTIVE
  To investigate the feasibility of the domestic porous tantalum as scaffold material of bone tissue engineering by observing the expressions of osteogenesis related factors of MG63 cells co-cultured with domestic porous tantalum.
  
  
  METHODS
  MG63 cells were cultured with porous tantalum scaffolds (group A), with porous tantalum leaching solution (group B), and with MEM as control group (group C). The cell adhesion of group A was observed on the scaffolds at 3, 5, and 7 days after culture by scanning electron microscopy (SEM); immunohistochemistry and Western blot methods were used to detect the expressions of Runt-related transcription factor 2 (Runx-2), osteocalcin (OC), and fibronectin (FN).
  
  
  RESULTS
  At 3 days after culture, the cells of group A adhered the surface and pore of the porous tantalum scaffolds, with sparse cell arrangement and less protuberances; at 5 days after culture, adjacent cells connected to be a flat each other, which covered the surface and pore of the scaffold; at 7 days after culture, cells secreted plenty of extracellular matrix, covering most of the material surface. The expressions of Runx-2, OC, and FN were positive in 3 groups; darker staining of the cytoplasm was observed in group A, the expressions were significantly higher in group A than in other 2 groups. The results of immunohistochemistry and Western blot showed that the expressions of Runx-2 and OC were significantly increased in group A when compared with those in groups B and C (P < 0.05), but no significant difference was found between groups B and C (P > 0.05). The expression of FN had no significant difference among 3 groups (P > 0.05).
  
  
  CONCLUSIONS
  Domestic porous tantalum could promote MG63 cells adhesion and growth, and may promote the expressions of Runx-2 and OC, so it can be used as a scaffold material of bone tissue engineering.
authors: Qian
authors: Q
authors: Wang
authors: Hui
authors: H
authors: Zhang
authors: Lixin
authors: L
authors: Geng
authors: Qijia
authors: Q
authors: Li
authors: Hongquan
authors: H
authors: Gan
authors: Hui
authors: H
authors: Wang
authors: Cheng
authors: C
authors: Bi
authors: Zhiqiang
authors: Z
authors: Wang
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D001842:Bone and Bones; D002478:Cells, Cultured / Q000378:metabolism; D018920:Coculture Techniques; D005109:Extracellular Matrix; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D013635:Tantalum / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D013635:Tantalum