title: Synthesis of Ca+2-Al+3-xanthan biopolymer particles and evaluation of in vitro protein release behaviors.
abstract: In this work, a hydrogelation method was used for the development of protein-loaded xanthan biopolymer particles. The gelation of the aqueous polymer droplets was accomplished in a mixture of calcium chloride and aluminium chloride solution. The particles were less than 900 μm in diameter and appeared spherical under scanning electron microscope. The particles retained a maximum of 90% of its initial load and released the protein molecules over an extended period in phosphate buffer saline solution (pH 7.4). The protein release was <13% in acidic medium (pH 1.2). The protein release was interrelated with pH-dependent swelling of the particles. The polymer relaxation phenomenon predominated over simple diffusion mechanism in anomalous protein release process as the ratio of di- and trivalent metal ions was decreased. No protein-biopolymer interaction was evident by FTIR spectroscopy. The divalent and trivalent metal ion cross-linked xanthan particles showed immense potential in controlled oral delivery of macromolecules.
authors: Gupta College of Technological Sciences, Ashram More, G.T. Road, Asansol 713301, West Bengal, India.
authors: Sabyasachi
authors: S
authors: Maiti
authors: Gupta College of Technological Sciences, Ashram More, G.T. Road, Asansol 713301, West Bengal, India. Electronic address: bibek_gcts@rediffmail.com.
authors: Bibek
authors: B
authors: Laha
authors: Gupta College of Technological Sciences, Ashram More, G.T. Road, Asansol 713301, West Bengal, India.
authors: Leena
authors: L
authors: Kumari
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D002417:Cattle; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001704:Biopolymers; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine; D000535:Aluminum; D002118:Calcium; C002563:xanthan gum
keywords: Biopolymer particles; Hydrogelation method; Polymer swelling; Protein entrapment efficiency; Protein release