title: The role of vascular endothelial growth factor receptor-1 signaling in compensatory contralateral lung growth following unilateral pneumonectomy.
abstract: Compensatory lung growth models have been widely used to investigate alveolization because the remaining lung can be kept intact and volume loss can be controlled. Vascular endothelial growth factor (VEGF) plays an important role in blood formation during lung growth and repair, but the precise mechanisms involved are poorly understood; therefore, the aim of this study was to investigate the role of VEGF signaling in compensatory lung growth. After left pneumonectomy, the right lung weight was higher in VEGF transgenic mice than wild-type (WT) mice. Compensatory lung growth was suppressed significantly in mice injected with a VEGF neutralizing antibody and in VEGF receptor-1 tyrosine kinase-deficient mice (TK(-/-) mice). The mobilization of progenitor cells expressing VEGFR1(+) cells from bone marrow and the recruitment of these cells to lung tissue were also suppressed in the TK(-/-) mice. WT mice transplanted with bone marrow from TK(-/-)transgenic GFP(+) mice had significantly lower numbers of GFP(+)/aquaporin 5(+), GFP(+)/surfactant protein A(+), and GFP(+)/VEGFR1(+) cells than WT mice transplanted with bone marrow from WTGFP(+) mice. The GFP(+)/VEGFR1(+) cells also co-stained for aquaporin 5 and surfactant protein A. Overall, these results suggest that VEGF signaling contributes to compensatory lung growth by mobilizing VEGFR1(+) cells.
authors: Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Yoshio
authors: Y
authors: Matsui
authors: 1] Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan [2] Department of Clinical Research Center, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Hideki
authors: H
authors: Amano
authors: Department of Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Yoshiya
authors: Y
authors: Ito
authors: Department of Immunology, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Koji
authors: K
authors: Eshima
authors: Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Hideaki
authors: H
authors: Tamaki
authors: Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Fumihiro
authors: F
authors: Ogawa
authors: Department of Thoracic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Akira
authors: A
authors: Iyoda
authors: Department of Molecular Oncology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Masafumi
authors: M
authors: Shibuya
authors: Department of Clinical Research Center, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Yuji
authors: Y
authors: Kumagai
authors: Department of Thoracic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Yukitoshi
authors: Y
authors: Satoh
authors: Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan.
authors: Masataka
authors: M
authors: Majima
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D016207:Cytokines / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology* / Q000601:surgery; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009929:Organ Size / Q000502:physiology; D011013:Pneumonectomy; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology; D015398:Signal Transduction / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D042461:Vascular Endothelial Growth Factor A; D011505:Protein-Tyrosine Kinases; D040281:Vascular Endothelial Growth Factor Receptor-1
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