title: Validated univariate and multivariate spectrophotometric methods for the determination of pharmaceuticals mixture in complex wastewater.
abstract: Five, accurate, precise, and sensitive univariate and multivariate spectrophotometric methods were developed for the simultaneous determination of a ternary mixture containing Trimethoprim (TMP), Sulphamethoxazole (SMZ) and Oxytetracycline (OTC) in waste water samples collected from different cites either production wastewater or livestock wastewater after their solid phase extraction using OASIS HLB cartridges. In univariate methods OTC was determined at its λmax 355.7 nm (0D), while (TMP) and (SMZ) were determined by three different univariate methods. Method (A) is based on successive spectrophotometric resolution technique (SSRT). The technique starts with the ratio subtraction method followed by ratio difference method for determination of TMP and SMZ. Method (B) is successive derivative ratio technique (SDR). Method (C) is mean centering of the ratio spectra (MCR). The developed multivariate methods are principle component regression (PCR) and partial least squares (PLS). The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures containing different ratios of the three drugs. The obtained results are statistically compared with those obtained by the official methods, showing no significant difference with respect to accuracy and precision at p=0.05.
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors: Safaa M
authors: SM
authors: Riad
authors: Analytical Chemistry Department, Faculty of Pharmacy, Deraya University, Menia, Egypt.
authors: Hesham
authors: H
authors: Salem
authors: Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October City, Egypt. Electronic address: heba_elbalkiny@hotmail.com.
authors: Heba T
authors: HT
authors: Elbalkiny
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors: Fatma I
authors: FI
authors: Khattab
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D016018:Least-Squares Analysis; D010118:Oxytetracycline / Q000032:analysis*; D025341:Principal Component Analysis; D013053:Spectrophotometry / Q000379:methods; D013420:Sulfamethoxazole / Q000032:analysis*; D014295:Trimethoprim / Q000032:analysis*; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D010118:Oxytetracycline; D013420:Sulfamethoxazole; D014295:Trimethoprim; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Mean centering of ratio spectra; Oxytetracycline; Successive derivative ratio; Sulphamethoxazole; Trimethoprim; Waste water