title: Proteomic analysis of ligamentum flavum from patients with lumbar spinal stenosis.
abstract: Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its molecular pathology has not been investigated using proteomics. Thus, we used several proteomic technologies to analyze the ligamentum flavum (LF) of individuals with LSS. Using comprehensive proteomics with strong cation exchange fractionation, we detected 1288 proteins in these LF samples. A GO analysis of the comprehensive proteome revealed that more than 30% of the identified proteins were extracellular. Next, we used 2D image converted analysis of LC/MS to compare LF obtained from individuals with LSS to that obtained from individuals with disc herniation (nondegenerative control). We detected 64 781 MS peaks and identified 1675 differentially expressed peptides derived from 286 proteins. We verified four differentially expressed proteins (fibronectin, serine protease HTRA1, tenascin, and asporin) by quantitative proteomics using SRM/MRM. The present proteomic study is the first to identify proteins from degenerated and hypertrophied LF in LSS, which will help in studying LSS.
authors: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan.
authors: Masahiro
authors: M
authors: Kamita
authors: Taiki
authors: T
authors: Mori
authors: Yoshihito
authors: Y
authors: Sakai
authors: Sadayuki
authors: S
authors: Ito
authors: Masahiro
authors: M
authors: Gomi
authors: Yuko
authors: Y
authors: Miyamoto
authors: Atsushi
authors: A
authors: Harada
authors: Shumpei
authors: S
authors: Niida
authors: Tesshi
authors: T
authors: Yamada
authors: Ken
authors: K
authors: Watanabe
authors: Masaya
authors: M
authors: Ono
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007405:Intervertebral Disc Displacement / Q000473:pathology; D017843:Ligamentum Flavum / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D013130:Spinal Stenosis / Q000473:pathology*
chemical_list: D020543:Proteome
keywords: 2D image converted analysis of LC/MS; Biomedicine; Ligamentum flavum; Lumbar spinal stenosis