title: Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines.
abstract: Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive elements, fragile sites and target genes. In HepG2, rhFIX was had an increased preference for chromosomes 6 and 17; the median distance to the nearest CpG islands was 15,240 base pairs and 37 % of the integrations occurred in RefSeq genes. In Hek293, rhFIX had an increased preference for chromosome 5; the median distance to the nearest CpG islands was 209,100 base pairs and 74 % of the integrations occurred in RefSeq genes. The integrations in both cell lines were distant from the TSS. The integration patterns associated with this vector are different in each cell line.
authors: Faculty of Medicine of Ribeirão Preto, Center for Cell Therapy and Regional Blood Center, University of São Paulo, Ribeirão Preto, Brazil.
authors: Andrielle
authors: A
authors: Castilho-Fernandes
authors: Aparecida Maria
authors: AM
authors: Fontes
authors: Kuruvilla Joseph
authors: KJ
authors: Abraham
authors: Marcela Cristina Corrêa
authors: MC
authors: de Freitas
authors: Nathalia Gonsales
authors: NG
authors: da Rosa
authors: Virginia
authors: V
authors: Picanço-Castro
authors: Elisa Maria
authors: EM
authors: de Sousa Russo-Carbolante
authors: Dimas Tadeu
authors: DT
authors: Covas
mesh_terms: D002460:Cell Line; D005164:Factor IX / Q000235:genetics* / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000502:physiology*; D008980:Moloney murine sarcoma virus / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D016662:Virus Integration
chemical_list: D011994:Recombinant Proteins; D005164:Factor IX