title: A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.
abstract: Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.
authors: Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Howon
authors: H
authors: Lee
authors: Celemics Inc., 371-17, Gasan-dong, Geumcheon-gu, Seoul 153-718, Republic of Korea.
authors: Hyoki
authors: H
authors: Kim
authors: 1] Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea [2] Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors: Sungsik
authors: S
authors: Kim
authors: 1] Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea [2] Department of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Republic of Korea.
authors: Taehoon
authors: T
authors: Ryu
authors: Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA.
authors: Hwangbeom
authors: H
authors: Kim
authors: Department of Chemistry, Yonsei University, Seoul 120-749, Republic of Korea.
authors: Duhee
authors: D
authors: Bang
authors: 1] Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea [2] Department of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Republic of Korea [3] Center for Nanoparticle Research, Institute for Basic Science, Seoul National University, Seoul 151-742, Republic of Korea [4] Seoul National University Hospital Biomedical Research Institute 101 Daehakro, Chungrogu, Seoul National University Hospital, Seoul 110-744, Republic of Korea.
authors: Sunghoon
authors: S
authors: Kwon
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA
references: Nature. 2011 Sep 22;477(7365):471-6
references: 21918511
references: Nat Biotechnol. 2011 May;29(5):449-52
references: 21516083
references: Science. 2008 Feb 29;319(5867):1215-20
references: 18218864
references: Nature. 2011 Jul 21;475(7356):348-52
references: 21776081
references: Cell. 1976 Sep;9(1):91-9
references: 788919
references: BMC Biotechnol. 2014;14:10
references: 24479830
references: Nucleic Acids Res. 2010 Oct;38(19):e180
references: 20693531
references: Nat Biotechnol. 2010 Dec;28(12):1295-9
references: 21113165
references: Nat Methods. 2012 Sep;9(9):913-5
references: 22886093
references: Science. 2009 Jan 2;323(5910):133-8
references: 19023044
references: Science. 2002 Aug 9;297(5583):1016-8
references: 12114528
references: Nat Biotechnol. 2010 Dec;28(12):1291-4
references: 21113166
references: Nucleic Acids Res. 2004;32(17):5011-8
references: 15448182
references: Science. 1980 Dec 5;210(4474):1081-8
references: 17831450
references: Science. 2010 Jan 1;327(5961):78-81
references: 19892942
references: Cell. 2006 Mar 24;124(6):1283-98
references: 16564017
references: Science. 2008 Apr 4;320(5872):106-9
references: 18388294
references: Science. 2010 Jul 2;329(5987):52-6
references: 20488990
references: Anal Chem. 2007 Jan 15;79(2):682-7
references: 17222037
references: Genome Biol. 2011;12(2):R18
references: 21338519
references: Nature. 2004 Dec 23;432(7020):1050-4
references: 15616567
references: Nature. 2005 Sep 15;437(7057):376-80
references: 16056220
references: Nature. 2008 Nov 6;456(7218):53-9
references: 18987734
references: Science. 2005 Sep 9;309(5741):1728-32
references: 16081699
references: Nucleic Acids Res. 2012 Oct;40(18):e140
references: 22705793