title: [Experiment study on the repair of rabbit skull defect with HAP-GEL scaffolds combined with cultured osteoblast].
abstract: OBJECTIVE
  To investigate the ability of composite graft of osteoblasts and HAP-GEL scaffolds to repair cranial bone defect.
  
  
  METHODS
  HAP-GEL and osteoblasts were co-cultured. The rabbit cranial parietal bone defect models were established. HAP-GEL scaffolds and osteoblasts were implanted into the cranial bone defect with blank defect as negative control, autologous skull as positive control. After 4,8,12 weeks, the result was evaluated by gross inspection, three-dimensional computed tomographic scanning and histological examination, respectively.
  
  
  RESULTS
  After 4, 8, 12 weeks, blank defects displayed significantly radiolucent area, HAP-GEL with osteoblasts and autologous skull restoration showed high density area, the edges were slightly blur. With time going by, part of the scaffolds were absorbed, and the new bone trabecular was observed in histological examination. In the control groups, only fibrous tissues were observed in the defect region, there was no new bone formation.
  
  
  CONCLUSIONS
  the engineering bone constructed by osteoblasts and HAP-GEL scaffold can be applied to repair bone defect.
authors: Hai-xia
authors: HX
authors: Geng
authors: Xiu-juan
authors: XJ
authors: Guo
authors: Jun-rong
authors: JR
authors: Qian
authors: Wei
authors: W
authors: Feng
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D010006:Osteoblasts; D011817:Rabbits; D012886:Skull; D023822:Tissue Engineering; D014945:Wound Healing