title: MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9.
abstract: MicroRNAs (miRNAs) represent a class of small non‑coding RNAs regulating gene expression by inducing the degradation of RNA or interfering with translation. Aberrant miRNA expression has been described in several types of cancer in humans. In the present study, it was demonstrated that miR‑145 is downregulated in pancreatic cancer tissues and the Panc‑1 cell line. Restoration of miR‑145 inhibited cell proliferation, invasion and migration in Panc‑1 cells. Neural precursor cell expressed, developmentally down‑regulated 9 (NEDD9) has been identified as a novel potential miR‑145 target using bioinformatics. Using luciferase reporter constructs, it was observed that the NEDD9 3'‑untranslated region is the location of the direct binding site for miR‑145. Additionally, it was identified that miR‑145 is inversely correlated with NEDD9 expression in pancreatic cancer tissues and that restoration of miR‑145 in Panc‑1 cells reduced NEDD9 mRNA and protein expression accompanied by inhibition of cell proliferation, invasion and migration. In conclusion, these findings indicate that miR‑145 may be an effective target for pancreatic cancer therapy.
authors: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors: Tong
authors: T
authors: Han
authors: Department of Radiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors: Xiao-Ping
authors: XP
authors: Yi
authors: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410008, P.R. China.
authors: Bo
authors: B
authors: Liu
authors: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors: Mu-Jing
authors: MJ
authors: Ke
authors: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors: Yi-Xiong
authors: YX
authors: Li
mesh_terms: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001483:Base Sequence; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D010183:Pancreatic Ducts / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D010750:Phosphoproteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C099942:NEDD9 protein, human; D010750:Phosphoproteins
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