title: NS5ATP9 Promotes Beclin 1-Dependent Starvation-Induced Autophagy of Hepatoblastoma Cells.
abstract: NS5ATP9, a gene up-regulated by NS5A, plays a crucial oncogenic role in several types of human tumours. However, the underlying mechanisms remain unclear. Autophagy, an evolutionarily conserved catabolic process, maintains cellular homeostasis under stress conditions, such as starvation, and plays a crucial role in tumour initiation and progression. Here, we report that NS5ATP9 mRNA and protein expression was up-regulated in starved HepG2 cells and that the up-regulated NS5ATP9 played a functional role in starvation-induced autophagy. Overexpression or silencing of this gene showed contrasting effects on Beclin 1 and on starvation-induced autophagy. Furthermore, NS5ATP9-mediated autophagy is required for promotion of tumour cell growth, and this effect could be inhibited with 3-methyladenine, chloroquine or by Beclin 1-silencing. Thus, the mechanism for NS5ATP9-promoted autophagy is Beclin 1-dependent in the condition of starvation, and for hepatoblastoma cell growth is also Beclin 1-dependent.
authors: Department of General Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Min
authors: M
authors: Quan
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Shunai
authors: S
authors: Liu
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Qi
authors: Q
authors: Wang
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Guoli
authors: G
authors: Li
authors: Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.
authors: Yu
authors: Y
authors: Zhang
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Shenghu
authors: S
authors: Feng
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Jinqiu
authors: J
authors: Liang
authors: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China.
authors: Jun
authors: J
authors: Cheng
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D001343:Autophagy; D000071186:Beclin-1; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002738:Chloroquine / Q000494:pharmacology; D004268:DNA-Binding Proteins; D056945:Hep G2 Cells; D018197:Hepatoblastoma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C425208:PCLAF protein, human; C025946:3-methyladenine; D002738:Chloroquine; D000225:Adenine
keywords: AUTOPHAGY; BECLIN 1; NS5ATP9; PROLIFERATION; STARVATION