title: Spatiotemporal characteristics and pharmacological modulation of multiple gamma oscillations in the CA1 region of the hippocampus.
abstract: Multiple components of "γ-oscillations" between 30-170 Hz in the CA1 region of the hippocampus have been described, based on their coherence with oscillations in other brain regions and on their cross-frequency coupling with local θ-oscillations. However, it remains unclear whether the different sub-bands are generated by a single broadband oscillator coupled to multiple external inputs, or by separate oscillators that incorporate distinct circuit elements. To distinguish between these possibilities, we used high-density linear array recording electrodes in awake behaving mice to examine the spatiotemporal characteristics of γ-oscillations and their responses to midazolam and atropine. We characterized oscillations using current source density (CSD) analysis, and measured θ-γ phase-amplitude coupling by cross frequency coupling (CFC) analysis. Prominent peaks were present in the CSD signal in the mid- and distal apical dendritic layers at all frequencies, and at stratum pyramidale for γ(slow) (30-45 Hz) and γ(mid) (50-90 Hz), but not γ(fast) (90-170 Hz) oscillations. Differences in the strength and timing of θ-γ(slow) and θ-γ(mid) cross frequency coupling, and a lack of coupling at the soma and mid-apical region for γ(fast) oscillations, indicated that separate circuit components generate the three sub-bands. Midazolam altered CSD amplitudes and cross-frequency coupling in a lamina- and frequency specific manner, providing further evidence for separate generator circuits. Atropine altered CSD amplitudes and θ-γ CFC uniformly at all locations. Simulations using a detailed compartmental model were consistent with γ(slow) and γ(mid) oscillations driven primarily by inputs at the mid-apical dendrites, and γ(fast) at the distal apical dendrite. Our results indicate that multiple distinct local circuits generate γ-oscillations in the CA1 region of the hippocampus, and provide detailed information about their spatiotemporal characteristics.
authors: Neuroscience Training Program, University of Wisconsin-Madison Madison, WI, USA ; Department of Anesthesiology, University of Wisconsin-Madison Madison, WI, USA.
authors: Shilpashree
authors: S
authors: Balakrishnan
authors: Department of Anesthesiology, University of Wisconsin-Madison Madison, WI, USA.
authors: Robert A
authors: RA
authors: Pearce
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000502:physiology*; D003198:Computer Simulation; D004567:Electrodes, Implanted; D018757:GABA Modulators / Q000494:pharmacology; D065818:Gamma Rhythm / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008822:Mice, Transgenic; D008874:Midazolam / Q000494:pharmacology; D008959:Models, Neurological; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D012815:Signal Processing, Computer-Assisted
chemical_list: D018757:GABA Modulators; D018727:Muscarinic Antagonists; D001285:Atropine; D008874:Midazolam
keywords: atropine; compartmental modeling; cross frequency coupling; current source density analysis; gamma oscillations; midazolam; theta oscillations
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