title: Prostatic inflammation induces urinary frequency in adult mice.
abstract: Lower urinary tract symptoms (LUTS) including urinary frequency and nocturia are common in aging men. Recent studies have revealed a strong association of prostatic inflammation with LUTS. We developed an animal model of bacterial induced, isolated prostatic inflammation and examined the effect of prostatic inflammation on voiding behavior in adult C57BL/6J mice. Prostatic inflammation was induced by transurethral inoculation of uropathogenic E. coli-1677. Bacterial cystitis was prevented by continuous administration of nitrofurantoin. Hematoxylin and eosin (H&E) staining and bacterial culture were preformed to validate our animal model. Voiding behavior was examined by metabolic cage testing on post-instillation day 1 (PID 1), PID 4, PID 7 and PID 14 and both voiding frequency and volume per void were determined. Mice with prostatic inflammation showed significantly increased voiding frequency at PID 1, 7 and 14, and decreased volume per void at all time points, as compared to mice instilled with saline and receiving nitrofurantoin (NTF). Linked analysis of voiding frequency and voided volumes revealed an overwhelming preponderance of high frequency, low volume voiding in mice with prostatic inflammation. These observations suggest that prostatic inflammation may be causal for symptoms of urinary frequency and nocturia.
authors: Department of Urology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America; Cellular and Molecular Biology Program, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America.
authors: Sanghee
authors: S
authors: Lee
authors: Department of Urology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America.
authors: Guang
authors: G
authors: Yang
authors: Department of Urology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America; Carbone Cancer Center, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, United States of America.
authors: Wade
authors: W
authors: Bushman
mesh_terms: D000818:Animals; D007249:Inflammation / Q000150:complications; D059411:Lower Urinary Tract Symptoms / Q000150:complications* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011467:Prostate / Q000382:microbiology / Q000473:pathology; D011469:Prostatic Diseases / Q000150:complications* / Q000382:microbiology / Q000473:pathology; D012669:Seminal Vesicles / Q000382:microbiology / Q000473:pathology; D014554:Urination
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