title: Development of penicillin G biosensor based on penicillinase enzymes immobilized onto bio-chips.
abstract: Penicillin G (Pen-G) biosensor was developed by immobilizing penicillinase enzymes (Pen-X) onto tiny bio-chips using thioglycolic acid self-assembled monolayer (TGA-SAM). The selective pen-G biosensor was investigated by ferri/ferrocyanide couple using electrochemical method for catalytic hydrolysis of Pen-G/Pen-X in a very sensible approach. Pen-G was detected with modified bio-chip (Gold electrode of bio-chip/Thioglycolic acid/Penicillinase enzyme, in shortly AuE/TGA/Pen-X) by reliable cyclic voltammetric method at pH 7.1 in room conditions. The AuE/TGA/Pen-X modified bio-chip sensor demonstrates good linearity (50.0 nM to 5.0 mM; R = 0.9987), low-detection limit (~1.26 nM, SNR ~ 3), and higher sensitivity (~4.97 μA.μM(-1).cm(-2)), lowest-small sample volume (<70.0 μL), good stability, selectivity, and reproducibility. To the best of our knowledge, this is the first statement in which such a very high sensitivity, low-detection limit, and low-sample volume are required for Pen-G biosensor using AuE/TGA/Pen-X bio-chips assembly. The results of these studies have introduced for biomedical applications with interesting assembly (AuE/TGA/Pen-X) towards the development of selective Pen-G biosensors. The AuE/TGA-SAM system was implemented a facile approach to the integration of Pen-X/Pen-G fabricated bio-chips, which can offer analytical access to a large group of enzymes for wide range of biomedical applications in health-care fields.
authors: Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia, mmrahman@kau.edu.sa.
authors: Mohammed M
authors: MM
authors: Rahman
authors: Abdullah M
authors: AM
authors: Asiri
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis*; D010405:Penicillinase / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D010405:Penicillinase; D010400:Penicillin G