title: [Impact of cryopreservation duration of 605 units umbilical cord blood on quality of hematopoietic stem cell and outcome of clinical transplantation].
abstract: OBJECTIVE
  To investigate the impact of cryopreservation duration of umbilical cord blood (UCB) on quality of hematopoietic stem cell and outcome of clinical transplantation.
  
  
  METHODS
  605 units of UCB which had been used in clinical transplantation were previously cryopreserved for 820 (88-2651) days in average. UCB was detected for total nucleated cell count, CD34+ cells count, cell recovery rate, cell viability and CFU-GM after thawing.
  
  
  RESULTS
  No statistical correlation was found between cryopreservation duration and cell recovery rate, cell viability. CFU-GM decreased along with the extension of cryopreservation duration (P=0.011), ranging between 109.6 and 105.7/1 × 10⁵. There was no significant difference on hematopoietic reconstitution time, graft failure, acute GVHD and overall survival among groups with different cryopreservation duration.
  
  
  CONCLUSIONS
  Cryopreservation duration has no significant effect on cell recovery rate, cell viability and clinical transplantation outcome. Extension of cryopreservation duration may reduce CFU-GM of stem cells with fluctaion still in normal range. UCB could maintain cell viability and function to achieve satisfactory clinical transplantation outcome even when thawed after 3 to 7 years' cryopreservation.
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Yi
authors: Y
authors: Zhang
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Hua
authors: H
authors: Zhu
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Huanying
authors: H
authors: Jin
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Yinting
authors: Y
authors: Wang
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Xiayan
authors: X
authors: Shao
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Jingsi
authors: J
authors: Kong
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Wenhao
authors: W
authors: Huang
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Yan
authors: Y
authors: Hong
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Chunli
authors: C
authors: Li
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Feng
authors: F
authors: Gao
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Liang
authors: L
authors: Chen
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Feng
authors: F
authors: Wang
authors: Shanghai Cord Blood Bank. Shanghai, Shanghai Stem Cell Technology Co., LTD. 200051, China.
authors: Yao
authors: Y
authors: Lu
mesh_terms: D002452:Cell Count; D002470:Cell Survival; D015925:Cryopreservation; D005312:Fetal Blood; D006086:Graft vs Host Disease; D055014:Granulocyte-Macrophage Progenitor Cells; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D016896:Treatment Outcome
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