title: Anti-inflammation effect of methyl salicylate 2-O-β-D-lactoside on adjuvant induced-arthritis rats and lipopolysaccharide (LPS)-treated murine macrophages RAW264.7 cells.
abstract: Methyl salicylate 2-O-β-D-lactoside (MSL) is a derivative of natural salicylate isolated from Gaultheria yunnanensis (Franch.) Rehder, which is widely used for treating rheumatoid arthritis (RA), swelling and pain. The aim of the present study was to investigate the effect of MSL on the progression of adjuvant-induced arthritis (AIA) in rat in vivo and explore the anti-inflammatory effects and mechanism of MSL in lipopolysaccharide (LPS)-treated murine macrophages RAW264.7 cells in vitro. Our results showed that MSL significantly inhibited the arthritis progression in AIA rats, decreasing the right hind paw swelling and ankle diameter, attenuating histopathological changes and suppressing the plasma levels of TNF-α and IL-1β in AIA rats. Besides, MSL had potent anti-inflammatory effects on the LPS-activated RAW264.7. MSL dose-dependently inhibited the activity of COX-1, and COX-2. Moreover, MSL prominently inhibited LPS-induced activation of MAPK in RAW264.7 cells by blocking phosphorylation of p38 and ERK. Our study suggests that MSL may be effective in the treatment of inflammatory diseases by inhibiting the pro-inflammatory cytokine production and regulating the MAPK signal pathway.
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: xuezhang@imm.ac.cn.
authors: Xue
authors: X
authors: Zhang
authors: Pharmacy Department of the Affiliated Hospital of Qingdao University, Qingdao 266003, China. Electronic address: sjlsyyk-412@163.com.
authors: Jialin
authors: J
authors: Sun
authors: Binzhou Medical University, Yantai 264003, China. Electronic address: xinwenyu1391139@163.com.
authors: Wenyu
authors: W
authors: Xin
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: leeyongjie@imm.ac.cn.
authors: Yongjie
authors: Y
authors: Li
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Lin
authors: L
authors: Ni
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Xiaowei
authors: X
authors: Ma
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Dan
authors: D
authors: Zhang
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Dongming
authors: D
authors: Zhang
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: ttzhang@imm.ac.cn.
authors: Tiantai
authors: T
authors: Zhang
authors: Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: dugh@imm.ac.cn.
authors: Guanhua
authors: G
authors: Du
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology; D002460:Cell Line; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D018450:Disease Progression; D031282:Gaultheria / Q000276:immunology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007785:Lactose / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008070:Lipopolysaccharides / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012459:Salicylates / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D012459:Salicylates; D014409:Tumor Necrosis Factor-alpha; C000597005:methyl salicylate 2-O-lactoside; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases; D007785:Lactose
keywords: Adjuvant-induced arthritis; Cyclooxygenase; MAPK signaling pathway; Methyl salicylate 2-O-β-D-lactoside; Prostaglandin E(2)