title: Proteomics beyond large-scale protein expression analysis.
abstract: Proteomics is commonly referred to as the application of high-throughput approaches to protein expression analysis. Typical results of proteomics studies are inventories of the protein content of a sample or lists of differentially expressed proteins across multiple conditions. Recently, however, an explosion of novel proteomics workflows has significantly expanded proteomics beyond the analysis of protein expression. Targeted proteomics methods, for example, enable the analysis of the fine dynamics of protein systems, such as a specific pathway or a network of interacting proteins, and the determination of protein complex stoichiometries. Structural proteomics tools allow extraction of restraints for structural modeling and identification of structurally altered proteins on a proteome-wide scale. Other variations of the proteomic workflow can be applied to the large-scale analysis of protein activity, location, degradation and turnover. These exciting developments provide new tools for multi-level 'omics' analysis and for the modeling of biological networks in the context of systems biology studies.
authors: Institute of Biochemistry, Department of Biology, ETH Zurich, Otto-Stern-Weg 3, CH-8093 Zurich, Switzerland.
authors: Paul J
authors: PJ
authors: Boersema
authors: Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
authors: Abdullah
authors: A
authors: Kahraman
authors: Institute of Biochemistry, Department of Biology, ETH Zurich, Otto-Stern-Weg 3, CH-8093 Zurich, Switzerland. Electronic address: paola.picotti@bc.biol.ethz.ch.
authors: Paola
authors: P
authors: Picotti
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D006801:Humans; D010766:Phosphorylation; D059748:Proteolysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D000596:Amino Acids; D020543:Proteome; D000255:Adenosine Triphosphate