title: Lipid accumulation by Rhodococcus rhodochrous grown on glucose.
abstract: Biodiesel is an alternative fuel made from costly vegetable oil feedstocks. Some microorganisms can accumulate lipids when nutrients are limited and carbon is in excess. Rhodococcus rhodochrous is a gram-positive bacterium most often used in bioremediation or acrylamide production. The purpose of this study was to investigate and characterize the lipid accumulation capabilities of R. rhodochrous. Shake flasks and a large-scale fermentation were used to cultivate R. rhodochrous in varying concentrations of glucose. R. rhodochrous achieved almost 50 % of dry cell mass as lipid when grown in 20 g/L of glucose. Wax esters and triglycerides were identified in R. rhodochrous lipid extract. The transesterified extractables of R. rhodochrous consisted of mostly palmitic (35 %) and oleic (42 %) acid methyl esters. This study shows R. rhodochrous to be an oleaginous bacterium with potential for application in alternative fuels.
authors: Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA.
authors: Sara A
authors: SA
authors: Shields-Menard
authors: Marta
authors: M
authors: Amirsadeghi
authors: Badamkhand
authors: B
authors: Sukhbaatar
authors: Emmanuel
authors: E
authors: Revellame
authors: Rafael
authors: R
authors: Hernandez
authors: Janet R
authors: JR
authors: Donaldson
authors: W Todd
authors: WT
authors: French
mesh_terms: D056804:Biofuels / Q000600:supply & distribution; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D009829:Oleic Acids / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D012240:Rhodococcus / Q000166:cytology / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D014885:Waxes / Q000378:metabolism
chemical_list: D056804:Biofuels; D008055:Lipids; D009829:Oleic Acids; D014280:Triglycerides; D014885:Waxes; D019308:Palmitic Acid; D002244:Carbon; D005947:Glucose
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