title: The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo.
abstract: We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achyranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Qiong
authors: Q
authors: Cheng
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Chunyi
authors: C
authors: Jiang
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Caiping
authors: C
authors: Wang
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Shu
authors: S
authors: Yu
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Qi
authors: Q
authors: Zhang
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Xiaosong
authors: X
authors: Gu
authors: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University, Nantong, Jiangsu Province, China.
authors: Fei
authors: F
authors: Ding
keywords: Achyranthes bidentata polypeptides; crush injury; dorsal root ganglion; nerve regeneration; neural regeneration; neurite outgrowth; neuroactive component; peripheral nerve regeneration; sciatic nerve
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