title: Reporters for sensitive and quantitative measurement of auxin response.
abstract: The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.
authors: Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
authors: Che-Yang
authors: CY
authors: Liao
authors: Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
authors: Wouter
authors: W
authors: Smet
authors: Laboratoire de Reproduction et Développement des Plantes, CNRS, Institut National de la Recherche Agronomique, Ecole Normale Superieure Lyon, Lyon, France.
authors: Geraldine
authors: G
authors: Brunoud
authors: Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
authors: Saiko
authors: S
authors: Yoshida
authors: Laboratoire de Reproduction et Développement des Plantes, CNRS, Institut National de la Recherche Agronomique, Ecole Normale Superieure Lyon, Lyon, France.
authors: Teva
authors: T
authors: Vernoux
authors: Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
authors: Dolf
authors: D
authors: Weijers
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D007210:Indoleacetic Acids; D008164:Luminescent Proteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D049452:Green Fluorescent Proteins
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