title: Effect of contact angle, zeta potential and particles size on the in vitro studies of Al2O3 and SiO2 nanoparticles.
abstract: Currently, nanometal oxides find their role in different biological applications such as tissue engineering, implant and bone replacement materials. Owing to the increased use of nanoparticles, it is necessary to understand their release and toxicity in the biological system. In this regard, three independent studies such as in vitro cytotoxicity, antioxidant activity and biocompatibility of nano- and micrometal oxide particles such as alumina (Al2O3) and silica (SiO2) are evaluated. It is evident from cell viability study that nanoAl2O3 and SiO2 particles are less toxic when compared with microAl2O3 and SiO2 to NIH 3T3 cell lines up to 200 µg/ml. Antioxidant properties of micro- and nanoAl2O3 in terms of radical scavenging percentage for micro- and nanoAl2O3 are 59.1% and 72.1%, respectively, at 100 mg. Similarly, the radical scavenging percentage of nano- and bulk SiO2 are 81.0% and 67.2%, respectively. The present study reveals that the cellular behaviour, interaction and biocompatibility of metal oxides differ with dose, particle size, contact angle and zeta potential. The present study opens up a new strategy to analyse in vitro nanotoxicity.
authors: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India.
authors: Gopalu
authors: G
authors: Karunakaran
authors: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India.
authors: Rangaraj
authors: R
authors: Suriyaprabha
authors: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India. veerajendran@gmail.com.
authors: Venkatachalam
authors: V
authors: Rajendran
authors: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode-637215, Tamil Nadu, India.
authors: Narayanasamy
authors: N
authors: Kannan
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008954:Models, Biological; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D012822:Silicon Dioxide; D000537:Aluminum Oxide