title: Human plasma and human platelet-rich plasma as a substitute for fetal calf serum during long-term cultivation of mesenchymal dental pulp stem cells.
abstract: OBJECTIVE
  Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties.
  
  
  METHODS
  DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically.
  
  
  RESULTS
  We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 ± 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 ± 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 ± 10.4 hours), 2% PRP (DT 40.1 ± 5.7 hours) and 2% HP (DT 49.0 ± 15.2 hours) showed almost the same proliferative activity. DPSC's viability in the 9th passage was over 90% except for the DPSC cultivated in the 10% HP media.
  
  
  CONCLUSIONS
  We proved that human blood components are suitable substitution for FCS in cultivation media for long-term DPSC cultivation.
authors: Department of Dentistry, Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic.
authors: Tereza
authors: T
authors: Suchánková Kleplová
authors: Department of Histology and Embryology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic.
authors: Tomáš
authors: T
authors: Soukup
authors: Transfusion Department, University Hospital, Hradec Králové, Czech Republic.
authors: Vít
authors: V
authors: Řeháček
authors: Department of Dentistry, Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic.
authors: Jakub
authors: J
authors: Suchánek
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D005312:Fetal Blood; D006801:Humans; D059630:Mesenchymal Stem Cells; D053657:Platelet-Rich Plasma; D013995:Time
chemical_list: D003470:Culture Media