title: Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs.
abstract: Forty-eight Duroc x Landrace x Large White gilts were used to determine the relationship between proteome changes of longissimus muscle and intramuscular fat (IMF) content in arginine-supplemented pigs. Beginning at 60 kg BW, pigs were fed a corn- and soybean meal-based diet supplemented or not with 1% L-arginine until they reached a BW of 100 kg. Supplementation with 1% L-arginine did not affect the growth performance or carcass traits, while it increased IMF content by 32% (P < 0.01), it also decreased the drip loss at 48 h post-mortem and the b* meat color value at 24 h post-mortem; supplementation with 1% dietary L-arginine did not change the proportion of SFA and MUFA in muscle lipids. The proteome changes in longissimus muscle between the control and supplemented pigs showed that L-arginine significantly influenced the abundance of proteins related to energy metabolism, fiber type and structure. The increase in IMF content was positively correlated with the increased abundance of slow twitch troponin I (TNNI1) protein and negatively correlated with myosin heavy chain IIb (MyHC IIb) protein content. It is suggested that the proteome changes in longissimus muscle contributed to the greater IMF content in L-arginine supplemented pigs.
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Xianyong
authors: X
authors: Ma
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Chuntian
authors: C
authors: Zheng
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Youjun
authors: Y
authors: Hu
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Li
authors: L
authors: Wang
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Xuefen
authors: X
authors: Yang
authors: Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
authors: Zongyong
authors: Z
authors: Jiang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D015153:Blotting, Western; D002452:Cell Count; D004032:Diet; D019587:Dietary Supplements; D005227:Fatty Acids / Q000378:metabolism; D048788:Growth and Development / Q000187:drug effects; D008460:Meat; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019655:Quantitative Trait, Heritable; D034421:Sus scrofa
chemical_list: D005227:Fatty Acids; D009124:Muscle Proteins; D020543:Proteome; D001120:Arginine
references: Mol Cell. 1999 Oct;4(4):611-7
references: 10549292
references: Gene. 2000 Jan 25;242(1-2):313-20
references: 10721725
references: J Cell Biol. 2002 May 27;157(5):831-8
references: 12034774
references: Biomed Pharmacother. 2002 Jul;56(5):215-22
references: 12199620
references: J Biol Chem. 2003 Sep 19;278(38):36603-10
references: 12857748
references: J Cell Sci. 2003 Oct 1;116(Pt 19):3893-904
references: 12915587
references: J Nutr. 2004 Mar;134(3):625-30
references: 14988458
references: Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7521-5
references: 1502163
references: J Nutr. 2005 Apr;135(4):714-21
references: 15795423
references: J Nutr Biochem. 2006 Sep;17(9):571-88
references: 16524713
references: J Anim Sci. 2006 Apr;84(4):918-24
references: 16543570
references: Trends Mol Med. 2006 May;12(5):189-92
references: 16595190
references: J Nutr. 2006 Jul;136(7):1759-62
references: 16772433
references: Mol Cell Biochem. 2007 Jan;294(1-2):163-71
references: 16838108
references: J Anim Sci. 2007 Apr;85(4):1074-9
references: 17121979
references: Mol Biol Cell. 2007 May;18(5):1723-33
references: 17332502
references: J Nutr Biochem. 2007 Nov;18(11):753-9
references: 17434723
references: Br J Nutr. 2008 Mar;99(3):455-61
references: 17697403
references: Physiol Genomics. 2007 Dec 19;32(1):58-63
references: 17848603
references: J Anim Sci. 2008 Apr;86(4):795-803
references: 18156349
references: J Nutr. 2008 Jan;138(1):60-6
references: 18156405
references: Amino Acids. 2009 May;37(1):169-75
references: 18683021
references: Amino Acids. 2009 May;37(1):199-208
references: 18989615
references: Amino Acids. 2010 Jan;38(1):95-102
references: 19057978
references: J Nutr. 2009 Feb;139(2):230-7
references: 19106310
references: J Pathol. 2009 Mar;217(4):497-506
references: 19156775
references: Amino Acids. 2009 May;37(1):187-98
references: 19212806
references: Biochem Biophys Res Commun. 2009 Apr 17;381(4):523-7
references: 19239903
references: J Nutr. 2009 Jul;139(7):1279-85
references: 19439462
references: BMB Rep. 2009 Jul 31;42(7):433-8
references: 19643041
references: J Appl Genet. 2010;51(1):51-7
references: 20145300
references: Meat Sci. 2009 Dec;83(4):744-51
references: 20416627
references: Amino Acids. 2010 Jul;39(2):349-57
references: 20437186
references: J Muscle Res Cell Motil. 1991 Feb;12(1):37-44
references: 2050810
references: J Nutr Biochem. 2011 May;22(5):441-5
references: 20619625
references: Meat Sci. 2010 Nov;86(3):764-9
references: 20663615
references: Meat Sci. 2010 Oct;86(2):324-30
references: 20665991
references: Meat Sci. 1994;38(2):269-77
references: 22059664
references: Meat Sci. 2000 Jun;55(2):187-95
references: 22061084
references: Meat Sci. 2007 Sep;77(1):46-54
references: 22061395
references: Meat Sci. 2005 Jan;69(1):79-91
references: 22062642
references: Meat Sci. 2003 May;64(1):93-103
references: 22062667
references: Meat Sci. 1999 Sep;53(1):59-65
references: 22062933
references: Meat Sci. 2005 Jun;70(2):307-17
references: 22063488
references: Meat Sci. 2007 Jun;76(2):281-8
references: 22064297
references: Med Oncol. 2012 Dec;29(4):2877-88
references: 22215415
references: J Proteomics. 2012 Apr 3;75(7):2093-108
references: 22270015
references: Amino Acids. 2012 Oct;43(4):1713-26
references: 22383090
references: Front Neuroenergetics. 2012 Mar 02;4:3
references: 22403540
references: Front Biosci (Landmark Ed). 2012 Jun 01;17:2237-46
references: 22652774
references: Curr Opin Clin Nutr Metab Care. 2012 Nov;15(6):529-38
references: 23075933
references: PLoS One. 2012;7(11):e49281
references: 23145141
references: Mamm Genome. 2013 Feb;24(1-2):72-9
references: 23160730
references: J Anim Sci. 2013 Feb;91(2):654-65
references: 23307841
references: Int J Biol Sci. 2013;9(1):108-18
references: 23355796
references: Biofactors. 2013 Jul-Aug;39(4):383-91
references: 23553707
references: Amino Acids. 2014 Feb;46(2):353-66
references: 24327170
references: J Anim Breed Genet. 2014 Aug;131(4):258-70
references: 24506540
references: Annu Rev Anim Biosci. 2014 Feb;2:387-417
references: 25384149
references: Differentiation. 1989 Dec;42(2):65-74
references: 2633939
references: Nature. 1979 Jun 28;279(5716):773-7
references: 450128
references: J Biol Chem. 1994 Apr 8;269(14):10651-9
references: 8144655
references: J Anim Sci. 1993 Nov;71(11):3011-9
references: 8270522
references: J Cell Biol. 1997 Oct 20;139(2):507-15
references: 9334352
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051