title: Effects of Toll-like receptor 3 on herpes simplex virus type-1-infected mouse neural stem cells.
abstract: In this study, we aimed to investigate the effect of herpes simplex virus type-1 (HSV-1) infection on the phosphorylation of interferon regulatory factor 3 (IRF3) and the expression of interferon-β (IFN-β), as well as to clarify the functions of toll-like receptor 3 (TLR3) in mouse neural stem cells (NSCs) infected with HSV-1. In HSV-1-infected cultured NSCs, immunofluorescence, reverse transcription - polymerase chain reaction, Western blot, and ELISA were performed to reveal the expression patterns of TLR3, IRF3, and IFN-β. Then, lentivirus-mediated RNA interference (RNAi) was used to block the expression of TLR3, and its effect on host resistance to HSV-1 infection was investigated. Under uninfected conditions, NSCs expressed TLR3 and phosphorylated IRF3, but after infection, the expression level of TLR3 was upregulated and the phosphorylation level of IRF3 in the nucleus was significantly enhanced, while IFN-β was also expressed. After TLR3 expression was blocked by lentivirus-mediated RNAi, IRF3 phosphorylation and IFN-β expression were downregulated. Therefore, HSV-1 upregulated the expression of TLR3 in NSCs and promoted nuclear translocation after IRF3 was phosphorylated to induce IFN-β expression. TLR3 exhibited an anti-HSV-1 infection capacity via innate immune functions.
authors: Department of Parasitology, Weifang Medical University, Shandong 261053, People's Republic of China.
authors: Xiuning
authors: X
authors: Sun
authors: Lihong
authors: L
authors: Shi
authors: Haoyun
authors: H
authors: Zhang
authors: Ruifang
authors: R
authors: Li
authors: Ruiwen
authors: R
authors: Liang
authors: Zhijun
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000378:metabolism* / Q000821:virology; D010766:Phosphorylation; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism*
chemical_list: D050838:Interferon Regulatory Factor-3; D051196:Toll-Like Receptor 3; D016899:Interferon-beta
keywords: IFN-β; cellule souche neurale; herpes simplex virus-1; immunité innée; infection; innate immune; interferon regulatory factor 3; neural stem cell; toll-like receptor; « interferon regulatory factor 3 »; « toll-like receptor 3 »