title: Interbreeding among deeply divergent mitochondrial lineages in the American cockroach (Periplaneta americana).
abstract: DNA barcoding promises to be a useful tool to identify pest species assuming adequate representation of genetic variants in a reference library. Here we examined mitochondrial DNA barcodes in a global urban pest, the American cockroach (Periplaneta americana). Our sampling effort generated 284 cockroach specimens, most from New York City, plus 15 additional U.S. states and six other countries, enabling the first large-scale survey of P. americana barcode variation. Periplaneta americana barcode sequences (n = 247, including 24 GenBank records) formed a monophyletic lineage separate from other Periplaneta species. We found three distinct P. americana haplogroups with relatively small differences within (≤0.6%) and larger differences among groups (2.4%-4.7%). This could be interpreted as indicative of multiple cryptic species. However, nuclear DNA sequences (n = 77 specimens) revealed extensive gene flow among mitochondrial haplogroups, confirming a single species. This unusual genetic pattern likely reflects multiple introductions from genetically divergent source populations, followed by interbreeding in the invasive range. Our findings highlight the need for comprehensive reference databases in DNA barcoding studies, especially when dealing with invasive populations that might be derived from multiple genetically distinct source populations.
authors: Laboratory of Insect Social Evolution, The Rockefeller University, New York, NY, USA.
authors: Christoph
authors: C
authors: von Beeren
authors: Program for the Human Environment, The Rockefeller University, New York, NY, USA.
authors: Mark Y
authors: MY
authors: Stoeckle
authors: 1] Laboratory of Insect Social Evolution, The Rockefeller University, New York, NY, USA [2] Hunter College High School, New York, NY, USA.
authors: Joyce
authors: J
authors: Xia
authors: 1] Laboratory of Insect Social Evolution, The Rockefeller University, New York, NY, USA [2] Bard College, Annandale-on-Hudson, NY, USA.
authors: Griffin
authors: G
authors: Burke
authors: Laboratory of Insect Social Evolution, The Rockefeller University, New York, NY, USA.
authors: Daniel J C
authors: DJ
authors: Kronauer
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic; D004272:DNA, Mitochondrial; D014644:Genetic Variation; D006239:Haplotypes; D007178:Inbreeding; D010526:Periplaneta / Q000145:classification* / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D058974:Phylogeography
chemical_list: D004272:DNA, Mitochondrial
references: Heredity (Edinb). 1999 Sep;83 ( Pt 3):260-70
references: 10504423
references: Annu Rev Entomol. 2002;47:33-55
references: 11729068
references: Environ Health Perspect. 2002 May;110(5):507-14
references: 12003754
references: Genetics. 2003 May;164(1):5-12
references: 12750316
references: Evolution. 2003 Jul;57(7):1631-52
references: 12940367
references: Proc Biol Sci. 2003 Aug 7;270 Suppl 1:S96-9
references: 12952648
references: Environ Health Perspect. 2003 Oct;111(13):1649-53
references: 14527845
references: Pest Manag Sci. 2003 Oct;59(10):1063-75
references: 14561063
references: Mol Ecol. 2003 Nov;12(11):3027-39
references: 14629383
references: Mol Ecol. 2004 Apr;13(4):729-44
references: 15012752
references: PLoS Biol. 2004 Oct;2(10):e312
references: 15455034
references: Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14812-7
references: 15465915
references: Heredity (Edinb). 2005 Apr;94(4):385
references: 15602569
references: Proc Biol Sci. 2005 Aug 7;272(1572):1525-34
references: 16048766
references: Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1813-23
references: 16214740
references: Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1847-57
references: 16214743
references: Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1859-68
references: 16221604
references: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):968-71
references: 16418261
references: Conserv Biol. 2006 Aug;20(4):1026-33
references: 16922219
references: Bioessays. 2007 Feb;29(2):188-97
references: 17226815
references: Trends Genet. 2007 Apr;23(4):167-72
references: 17316886
references: Front Zool. 2007 Mar 07;4:8
references: 17343734
references: Mol Phylogenet Evol. 2007 Sep;44(3):1346-51
references: 17350292
references: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3883-8
references: 17360447
references: Proc Biol Sci. 2007 Jul 22;274(1619):1731-9
references: 17472911
references: Trends Ecol Evol. 2007 Sep;22(9):454-64
references: 17673331
references: Mol Phylogenet Evol. 2007 Nov;45(2):534-46
references: 17693098
references: Proc Biol Sci. 2007 Nov 22;274(1627):2881-9
references: 17785265
references: Mol Ecol. 2008 Jan;17(1):431-49
references: 17908213
references: Evolution. 2007 Dec;61(12):2879-97
references: 17941840
references: Mol Ecol. 2008 Feb;17(3):773-82
references: 18194168
references: Mol Phylogenet Evol. 2008 Sep;48(3):877-91
references: 18644735
references: Annu Rev Genet. 2008;42:683-707
references: 18713031
references: Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12359-64
references: 18716001
references: Mol Ecol Notes. 2007 May 1;7(3):355-364
references: 18784790
references: Syst Biol. 2008 Oct;57(5):809-13
references: 18853366
references: Mol Biol Evol. 2010 Jul;27(7):1659-72
references: 20167609
references: Proc Biol Sci. 2011 Jan 7;278(1702):2-8
references: 20685700
references: Bioessays. 2011 Feb;33(2):135-47
references: 21184470
references: J Evol Biol. 2011 May;24(5):1044-52
references: 21342302
references: Syst Biol. 2011 Jul;60(4):482-502
references: 21471306
references: Mol Ecol Resour. 2009 May;9 Suppl s1:140-50
references: 21564973
references: Biol Lett. 2012 Jun 23;8(3):430-3
references: 22130172
references: BMC Evol Biol. 2011 Dec 12;11:358
references: 22151691
references: Evolution. 2012 Jun;66(6):1907-16
references: 22671555
references: Methods Mol Biol. 2012;858:269-310
references: 22684961
references: BMC Genet. 2013 Mar 09;14:20
references: 23497346
references: Sci Rep. 2013;3:2635
references: 24022383
references: Annu Rev Entomol. 1990;35:521-51
references: 2405773
references: Mol Biol Evol. 2013 Dec;30(12):2725-9
references: 24132122
references: PLoS One. 2013 Nov 06;8(11):e78107
references: 24223136
references: Bioinformatics. 2014 May 1;30(9):1312-3
references: 24451623
references: Zookeys. 2013 Dec 30;(365):235-44
references: 24453560
references: J Econ Entomol. 2013 Dec;106(6):2275-9
references: 24498724
references: PLoS One. 2014 Mar 07;9(3):e90653
references: 24609003
references: Mol Ecol Resour. 2015 Mar;15(2):268-77
references: 25042335
references: Evol Appl. 2011 Jan;4(1):71-88
references: 25567954
references: Evolution. 1984 Nov;38(6):1358-1370
references: 28563791
references: Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
references: 7881515