title: A neuroprotective role for microRNA miR-1000 mediated by limiting glutamate excitotoxicity.
abstract: Evidence has begun to emerge for microRNAs as regulators of synaptic signaling, specifically acting to control postsynaptic responsiveness during synaptic transmission. In this report, we provide evidence that Drosophila melanogaster miR-1000 acts presynaptically to regulate glutamate release at the synapse by controlling expression of the vesicular glutamate transporter (VGlut). Genetic deletion of miR-1000 led to elevated apoptosis in the brain as a result of glutamatergic excitotoxicity. The seed-similar miR-137 regulated VGluT2 expression in mouse neurons. These conserved miRNAs share a neuroprotective function in the brains of flies and mice. Drosophila miR-1000 showed activity-dependent expression, which might serve as a mechanism to allow neuronal activity to fine-tune the strength of excitatory synaptic transmission.
authors: 1] Institute of Molecular and Cell Biology, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore.
authors: Pushpa
authors: P
authors: Verma
authors: 1] Institute of Molecular and Cell Biology, Singapore. [2] Duke-NUS Graduate Medical School, Singapore. [3] Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, Republic of Korea. [4] Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors: George J
authors: GJ
authors: Augustine
authors: 1] Warwick-Nanyang Technological University Neuroscience Programme, School of Biological Sciences, Nanyang Technological University, Singapore. [2] School of Life Sciences, University of Warwick, Coventry, UK.
authors: Mohamed-Raafet
authors: MR
authors: Ammar
authors: 1] Warwick-Nanyang Technological University Neuroscience Programme, School of Biological Sciences, Nanyang Technological University, Singapore. [2] School of Life Sciences, University of Warwick, Coventry, UK.
authors: Ayumu
authors: A
authors: Tashiro
authors: 1] Institute of Molecular and Cell Biology, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore.
authors: Stephen M
authors: SM
authors: Cohen
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000517:prevention & control*; D009435:Synaptic Transmission / Q000187:drug effects / Q000235:genetics; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C558262:MIRN100 microRNA, Drosophila; D035683:MicroRNAs; C558180:Mirn100 microRNA, mouse; C493477:Slc17a6 protein, mouse; D050599:Vesicular Glutamate Transport Protein 2
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