title: Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach.
abstract: Despite the importance of saliva in the regulation of oral cavity homeostasis, few studies have been conducted to quantitatively compare the saliva of different mammal species. Aiming to define a proteome signature of mammals' saliva, an in-depth SDS-PAGE-LC coupled to MS/MS (GeLC-MS/MS) approach was used to characterize the saliva from primates (human), carnivores (dog), glires (rat and rabbit), and ungulates (sheep, cattle, horse). Despite the high variability in the number of distinct proteins identified per species, most protein families were shared by the mammals studied with the exception of cattle and horse. Alpha-amylase is an example that seems to reflect the natural selection related to digestion efficacy and food recognition. Casein protein family was identified in all species but human, suggesting an alternative to statherin in the protection of hard tissues. Overall, data suggest that different proteins might assure a similar role in the regulation of oral cavity homeostasis, potentially explaining the specific mammals' salivary proteome signature. Moreover, some protein families were identified for the first time in the saliva of some species, the presence of proline-rich proteins in rabbit's saliva being a good example.
authors: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal; CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, Vairão, Portugal.
authors: Patrícia
authors: P
authors: de Sousa-Pereira
authors: Marta
authors: M
authors: Cova
authors: Joana
authors: J
authors: Abrantes
authors: Rita
authors: R
authors: Ferreira
authors: Fábio
authors: F
authors: Trindade
authors: António
authors: A
authors: Barros
authors: Pedro
authors: P
authors: Gomes
authors: Bruno
authors: B
authors: Colaço
authors: Francisco
authors: F
authors: Amado
authors: Pedro J
authors: PJ
authors: Esteves
authors: Rui
authors: R
authors: Vitorino
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D004285:Dogs; D006736:Horses; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000737:chemistry*; D012756:Sheep; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D020543:Proteome
keywords: Animal proteomics; Carnivorous; GeLC-MS/MS; Glires; Primates; Ungulates