title: Matrigel basement membrane matrix induces eccrine sweat gland cells to reconstitute sweat gland-like structures in nude mice.
abstract: BACKGROUND
  Severe burn results in irreversible damage to eccrine sweat glands, for which no effective treatment is available. Interaction between the extracellular matrix and epithelial cells is critical for proper three-dimensional organization and function of the epithelium.
  
  
  METHODS
  Matrigel-embedded eccrine sweat gland cells were subcutaneously implanted into the inguinal regions of nude mice. Two weeks later, the Matrigel plugs were removed and evaluated for series of detection items.
  
  
  RESULTS
  Sweat gland cells developed into sweat gland-like structures in the Matrigel plugs based on: (1) de novo formation of tubular-like structures with one or more hollow lumens, (2) expression of epithelial and sweat gland markers (pancytokeratin, CK5/7/14/19, α-SMA and CEA), (3) basement membrane formation, (4) myoepithelial cells presenting in and encompassing the tubular-like structures, (5) cellular polarization, evident by the expression of tight junction proteins (claudin-1 and ZO-2), anchoring junctions (desmoglein-1 and -2 and E-cadherin) and CEA in the luminal membrane, (6) expression of proteins related to sweat secretion and absorption (Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotranspoter 1, Na(+)/H(+) exchanger 1, aquaporin-5, epithelial sodium channel, cystic fibrosis transmembrane conductance regulator, potassium channel and vacuolar-type H+-ATPase), and (7) about 20% of the tubular-like structures are de novo coils and 80% are de novo ducts.
  
  
  CONCLUSIONS
  This study provides not only an excellent model to study eccrine sweat gland development, cytodifferentiation and reconstitution, but also an in vivo model for regeneration of eccrine sweat glands.
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China. Electronic address: lihaihong1051@126.com.
authors: Haihong
authors: H
authors: Li
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Lu
authors: L
authors: Chen
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Shaopeng
authors: S
authors: Zeng
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Xuexue
authors: X
authors: Li
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Xiang
authors: X
authors: Zhang
authors: Department of Histology and Embryology, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong Province 515041, PR China.
authors: Changmin
authors: C
authors: Lin
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Mingjun
authors: M
authors: Zhang
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Sitian
authors: S
authors: Xie
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Yunpu
authors: Y
authors: He
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Shenyou
authors: S
authors: Shu
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Lvjun
authors: L
authors: Yang
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
authors: Shijie
authors: S
authors: Tang
authors: Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical School, 51 Fucheng Road, Beijing 100037, PR China.
authors: Xiaobing
authors: X
authors: Fu
mesh_terms: D000293:Adolescent; D000818:Animals; D001485:Basement Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D003470:Culture Media; D004338:Drug Combinations; D004439:Eccrine Glands / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011509:Proteoglycans / Q000737:chemistry*; D012038:Regeneration; D013542:Sweat / Q000378:metabolism; D055815:Young Adult
chemical_list: D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: Eccrine sweat gland; Matrigel basement membrane matrix; Nude mice; Reconstitution; Sweat gland-like structure