title: Identification and development of a functional marker from6-SFT-A2associated with grain weight in wheat.
abstract: As a class of water-soluble, fructose-based oligo- and polysaccharides, fructans are major nonstructural carbohydrates and an important carbon source for grain filling in wheat (Triticum aestivumL.). Four enzymes are involved in fructan synthesis in higher plants, and 6-SFT is a key enzyme in fructan biosynthesis. In this study, thirteen single nucleotide polymorphisms were detected in6-SFT-A2in 24 wheat accessions, forming three haplotypes. Two cleaved amplified polymorphic sequence markers developed based on polymorphisms at sites 1870(A-G) and 1951(A-G) distinguished the three haplotypes.6-SFT-A2was located on chromosome 4A, between markersP2454.3andP3465.1in a doubled haploid (DH) population derived from the cross Hanxuan 10 × Lumai 14. The DH population comprising 150 lines and a historical population consisting of 154 accessions were used in a6-SFT-A2marker-trait association analysis. The three haplotypes were significantly associated with thousand-grain weight (TGW) under rainfed conditions.HapIII had a significant positive effect on TGW. There were significant differences between the Hanxuan 10 and Lumai 14 genotypes in both rainfed and irrigated environments. The average TGW of Lumai 14 (HapIII) was higher than that of Hanxuan 10 (HapI). The frequencies of6-SFT-A2HapIII in cultivars released at different periods showed that it had been strongly positively selected in breeding programs. The preferredHapIII for TGW occurred at higher frequencies in Gansu, Beijing, Shanxi, and Hebei than other regions in northern China.
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China ; Agronomy College, Shanxi Agricultural University, Taigu, 030801 China.
authors: Aiqin
authors: A
authors: Yue
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
authors: Ang
authors: A
authors: Li
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
authors: Xinguo
authors: X
authors: Mao
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
authors: Xiaoping
authors: X
authors: Chang
authors: Agronomy College, Shanxi Agricultural University, Taigu, 030801 China.
authors: Runzhi
authors: R
authors: Li
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
authors: Ruilian
authors: R
authors: Jing
keywords: 6-SFT; Association analysis; Haplotype; Marker development; Thousand-grain weight; Triticum aestivum
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