title: The viability and protein expression of Beijing/W lineage Mycobacterium tuberculosis circulating in Xinjiang, China.
abstract: Beijing/W lineage strains of Mycobacterium tuberculosis spread faster than other strains, tend to be more virulent and frequently associated with drug resistance. In this study, to distinguish the characteristics of Beijing/W lineage and non-Beijing/W lineage M. tuberculosis, we assessed the growth between the two groups under conditions of hypoxia, nutrient starvation, and intracellular growth in murine macrophages. We also examined the DNA, RNA, and protein levels of 5 major M. tuberculosis proteins, including HspX, Hsp65, 38 kDa, Ag85B, and MPT64 of the different types of strains by sequencing, quantitative RT-PCR, and Western blotting. The results showed that Beijing/W and non-Beijing/W lineage strains of M. tuberculosis have similar viability in ex vivo culture but differ in their ability to survive within macrophages, and the intracellular viability of the Beijing/W lineage strains was significantly more than the viability of the non-Beijing/W lineage strains at 2, 3, and 5 days after infection (P < 0.05). Psts1 and fbpB were expressed at statistically lower levels in Beijing/W lineage strains in their mRNA expression levels (P < 0.05). The expression of their corresponding 38 kDa and Ag85B was lower in the Beijing/W lineage strains than the non-Beijing/W lineage strains (P < 0.05). The expression of HspX and Hsp65 was higher in the Beijing/W lineage strains in their protein expression levels at 24 h after infection of RAW264.7 macrophages (P < 0.05). In conclusion, the increased viability of the Beijing/W lineage strains might be related to the expression levels of these proteins.
authors: Department of Microbiology and Immunology, Xinjiang Laboratory of Endemic and Ethnic Disease, Shihezi University, Shihezi, 832000, China, lf703@163.com.
authors: Feng
authors: F
authors: Li
authors: Hua
authors: H
authors: Li
authors: Wei-Ze
authors: WZ
authors: Zuo
authors: Ligu
authors: L
authors: Mi
authors: Xian
authors: X
authors: Wang
authors: Yuanzhi
authors: Y
authors: Wang
authors: Hong
authors: H
authors: Wang
authors: Aiping
authors: A
authors: Shen
authors: Shuaili
authors: S
authors: Cao
authors: Li
authors: L
authors: Yuan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D050296:Microbial Viability; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial
references: Infect Immun. 1999 Nov;67(11):6187-90
references: 10531287
references: Eur J Biochem. 2000 Jul;267(13):4028-32
references: 10866802
references: Annu Rev Microbiol. 2001;55:139-63
references: 11544352
references: Mol Microbiol. 2002 Feb;43(3):717-31
references: 11929527
references: J Clin Microbiol. 2003 Apr;41(4):1637-50
references: 12682155
references: Tuberculosis (Edinb). 2004;84(1-2):29-44
references: 14670344
references: Infect Immun. 1998 Jan;66(1):176-80
references: 9423855
references: J Infect Dis. 1999 May;179(5):1213-7
references: 10191225
references: Nature. 1999 Jul 15;400(6741):269-71
references: 10421369
references: J Biol Chem. 2005 Feb 25;280(8):6337-48
references: 15545279
references: J Clin Microbiol. 2005 Jul;43(7):3185-91
references: 16000433
references: BMC Microbiol. 2006;6:23
references: 16519816
references: Scand J Immunol. 2009 Jan;69(1):20-8
references: 19140873
references: Clin Vaccine Immunol. 2010 Mar;17(3):372-5
references: 20053875
references: PLoS Pathog. 2011 Jun;7(6):e1002093
references: 21731490
references: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Dec;27(12):1301-3
references: 22152810
references: Zhonghua Yu Fang Yi Xue Za Zhi. 2012 Feb;46(2):158-64
references: 22490200