title: Influence of reactive species on the modification of biomolecules generated from the soft plasma.
abstract: Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N2 and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H2O2 with Hb and Mb, which reveals that H2O2 molecules preferably attack the amino acids near heme group. We have also shown that N2 gas plasma has strong deformation action on biomolecules and compared to other gases plasma.
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
authors: Pankaj
authors: P
authors: Attri
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
authors: Naresh
authors: N
authors: Kumar
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
authors: Ji Hoon
authors: JH
authors: Park
authors: Laboratory of Nanoscale Characterization &Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
authors: Dharmendra Kumar
authors: DK
authors: Yadav
authors: Department of Biochemistry, College of Life Science &Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Sooho
authors: S
authors: Choi
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
authors: Han S
authors: HS
authors: Uhm
authors: Department of Chemistry, Kwangwoon University, Seoul, Korea 139-701.
authors: In Tae
authors: IT
authors: Kim
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
authors: Eun Ha
authors: EH
authors: Choi
authors: Department of Biochemistry, College of Life Science &Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Weontae
authors: W
authors: Lee
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry; D013058:Mass Spectrometry; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D011485:Protein Binding; D017433:Protein Structure, Secondary; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D009211:Myoglobin; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D004247:DNA
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