title: The networks of human gut microbe-metabolite associations are different between health and irritable bowel syndrome.
abstract: The goal of this study was to determine if fecal metabolite and microbiota profiles can serve as biomarkers of human intestinal diseases, and to uncover possible gut microbe-metabolite associations. We employed proton nuclear magnetic resonance to measure fecal metabolites of healthy children and those diagnosed with diarrhea-predominant irritable bowel syndrome (IBS-D). Metabolite levels were associated with fecal microbial abundances. Using several ordination techniques, healthy and irritable bowel syndrome (IBS) samples could be distinguished based on the metabolite profiles of fecal samples, and such partitioning was congruent with the microbiota-based sample separation. Measurements of individual metabolites indicated that the intestinal environment in IBS-D was characterized by increased proteolysis, incomplete anaerobic fermentation and possible change in methane production. By correlating metabolite levels with abundances of microbial genera, a number of statistically significant metabolite-genus associations were detected in stools of healthy children. No such associations were evident for IBS children. This finding complemented the previously observed reduction in the number of microbe-microbe associations in the distal gut of the same cohort of IBS-D children.
authors: Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
authors: Vijay
authors: V
authors: Shankar
authors: Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
authors: Daniel
authors: D
authors: Homer
authors: Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
authors: Laura
authors: L
authors: Rigsbee
authors: Department of Mathematics and Statistics, Wright State University, Dayton, OH, USA.
authors: Harry J
authors: HJ
authors: Khamis
authors: Department of Pediatrics, Wright State University, Dayton, OH, USA.
authors: Sonia
authors: S
authors: Michail
authors: Department of Computer Sciences, Wright State University, Dayton, OH, USA.
authors: Michael
authors: M
authors: Raymer
authors: Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
authors: Nicholas V
authors: NV
authors: Reo
authors: Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
authors: Oleg
authors: O
authors: Paliy
mesh_terms: D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005260:Female; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000378:metabolism / Q000382:microbiology*; D008297:Male; D064307:Microbiota
chemical_list: D015415:Biomarkers
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