title: Loss of Angiopoietin-like 7 diminishes the regeneration capacity of hematopoietic stem and progenitor cells.
abstract: Successful expansion of hematopoietic stem cells (HSCs) would benefit the use of HSC transplants in the clinic. Angiopoietin-like 7 promotes the expansion of hematopoietic stem and progenitor cells (HSPC) in vitro and ex vivo. However, the impact of loss of Angptl7 on HSPCs in vivo has not been characterized. Here, we generated Angptl7-deficient mice by TALEN-mediated gene targeting and found that HSC compartments in Angptl7-null mice were compromised. In addition, wild type (WT) HSPCs failed to repopulate in the BM of Angptl7-null mice after serial transplantations while the engraftment of Angptl7-deficient HSPCs in WT mice was not impaired. These results suggest that Angptl7 is required for HSPCs repopulation in a non-cell autonomous manner.
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. xiao_yiren@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. xiao_yiren@gibh.ac.cn.
authors: Yiren
authors: Y
authors: Xiao
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. wei_xinru@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. wei_xinru@gibh.ac.cn.
authors: Xinru
authors: X
authors: Wei
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. jiang_zhiwu@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. jiang_zhiwu@gibh.ac.cn.
authors: Zhiwu
authors: Z
authors: Jiang
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China. helenwxm@sina.com.
authors: Xiangmeng
authors: X
authors: Wang
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. ye_wei@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. ye_wei@gibh.ac.cn.
authors: Wei
authors: W
authors: Ye
authors: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, 518055, China. xin.liu@siat.ac.cn.
authors: Xin
authors: X
authors: Liu
authors: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, 518055, China. minjie.zhang@gmail.com.
authors: Minjie
authors: M
authors: Zhang
authors: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. sau_xuyan@163.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. sau_xuyan@163.com.
authors: Yan
authors: Y
authors: Xu
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. wu_donghai@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. wu_donghai@gibh.ac.cn.
authors: Donghai
authors: D
authors: Wu
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. lai_liangxue@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. lai_liangxue@gibh.ac.cn.
authors: Liangxue
authors: L
authors: Lai
authors: Department of Outpatient, The 91th Military Hospital, 454003, Jiaozuo, China. jiefangjun118@sina.com.
authors: Huihui
authors: H
authors: Yao
authors: Division of Reproductive Endocrinology, The 91th Military Hospital, 454003, Jiaozuo, China. 18639157796@163.com.
authors: Zixia
authors: Z
authors: Liu
authors: Division of General Pediatrics, The 91th Military Hospital, 454003, Jiaozuo, China. jfj91zxyyek@yeah.com.
authors: Su
authors: S
authors: Cao
authors: Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, England, UK. pl2@sanger.ac.uk.
authors: Pentao
authors: P
authors: Liu
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China. xubingzhangjian@126.com.
authors: Bing
authors: B
authors: Xu
authors: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors: Yangqiu
authors: Y
authors: Li
authors: Drug Discovery Pipeline, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. yao_yao@gibh.ac.cn.
authors: Yao
authors: Y
authors: Yao
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. pei_duanqing@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. pei_duanqing@gibh.ac.cn.
authors: Duanqing
authors: D
authors: Pei
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Science Park, Guangzhou, 7, Guangdong, 510530, China. li_peng@gibh.ac.cn.|Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. li_peng@gibh.ac.cn.
authors: Peng
authors: P
authors: Li
mesh_terms: D000091307:Angiopoietin-Like Protein 7; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000378:metabolism*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D060833:Cellular Microenvironment / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D051379:Mice; D018345:Mice, Knockout
chemical_list: C000599407:ANGPTL7 protein, mouse; D000091307:Angiopoietin-Like Protein 7; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins
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