title: In vitro plant regeneration of Aster scaber via somatic embryogenesis.
abstract: We established an in vitro plant regeneration system via somatic embryogenesis of Aster scaber, an important source of various biologically active phytochemicals. We examined the callus induction and embryogenic capacities of three explants, including leaves, petioles, and roots, on 25 different media containing different combinations of α-naphthalene acetic acid (NAA) and 6-benzyladenine (BA). The optimum concentrations of NAA and BA for the production of embryogenic calli were 5.0 μM and 0.05 μM, respectively. Media containing higher concentrations of auxin and cytokinin (such as 25 μM NAA and 25 μM BA) were suitable for shoot regeneration, especially for leaf-derived calli, which are the most readily available calli and are highly competent. For root induction from regenerated shoots, supplemental auxin and/or cytokinin did not improve rooting, but instead caused unwanted callus induction or retarded growth of regenerated plants. Therefore, plant growth regulator-free medium was preferable for root induction. Normal plants were successfully obtained from calli under the optimized conditions described above. This is the first report of the complete process of in vitro plant regeneration of A. scaber via somatic embryogenesis.
authors: a Department of Biotechnology, College of Applied Life Science (SARI) , Jeju National University , Jeju , Republic of Korea.
authors: Kyung Hwan
authors: KH
authors: Boo
authors: Dang Viet
authors: DV
authors: Cao
authors: Reniel S
authors: RS
authors: Pamplona
authors: Doseung
authors: D
authors: Lee
authors: Key-Zung
authors: KZ
authors: Riu
authors: Dong-Sun
authors: DS
authors: Lee
mesh_terms: D031173:Aster Plant / Q000187:drug effects / Q000502:physiology*; D001593:Benzyl Compounds; D066298:In Vitro Techniques; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D011687:Purines; D012038:Regeneration; D012987:Soil
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D012987:Soil; C034182:1-naphthaleneacetic acid; C480551:benzylaminopurine; D007701:Kinetin
keywords: Aster scaber; plant regeneration; somatic embryogenesis; tissue culture