title: Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield.
abstract: Geranylgeranyl pyrophosphate synthase (GGPS) is a key enzyme for a structurally diverse class of isoprenoid biosynthetic metabolites including gibberellins, carotenoids, chlorophylls and rubber. We expressed a chloroplast-targeted GGPS isolated from sunflower (Helianthus annuus) under control of the cauliflower mosaic virus 35S promoter in tobacco (Nicotiana tabacum). The resulting transgenic tobacco plants expressing heterologous GGPS showed remarkably enhanced growth (an increase in shoot and root biomass and height), early flowering, increased number of seed pods and greater seed yield compared with that of GUS-transgenic lines (control) or wild-type plants. The gibberellin levels in HaGGPS-transgenic plants were higher than those in control plants, indicating that the observed phenotype may result from increased gibberellin content. However, in HaGGPS-transformant tobacco plants, we did not observe the phenotypic defects such as reduced chlorophyll content and greater petiole and stalk length, which were previously reported for transgenic plants expressing gibberellin biosynthetic genes. Fast plant growth was also observed in HaGGPS-expressing Arabidopsis and dandelion plants. The results of this study suggest that GGPS expression in crop plants may yield desirable agronomic traits, including enhanced growth of shoots and roots, early flowering, greater numbers of seed pods and/or higher seed yield. This research has potential applications for fast production of plant biomass that provides commercially valuable biomaterials or bioenergy.
authors: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, Korea.|Biosystems & Bioengineering Division, University of Science and Technology, Daejeon, Korea.
authors: Sandeep Kumar
authors: SK
authors: Tata
authors: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, Korea.|Biosystems & Bioengineering Division, University of Science and Technology, Daejeon, Korea.
authors: Jihye
authors: J
authors: Jung
authors: School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
authors: Yoon-Ha
authors: YH
authors: Kim
authors: School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors: Jun Young
authors: JY
authors: Choi
authors: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, Korea.
authors: Ji-Yul
authors: JY
authors: Jung
authors: School of Applied Biosciences, Kyungpook National University, Daegu, Korea.
authors: In-Jung
authors: IJ
authors: Lee
authors: School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors: Jeong Sheop
authors: JS
authors: Shin
authors: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, Korea.|Biosystems & Bioengineering Division, University of Science and Technology, Daejeon, Korea.
authors: Stephen Beungtae
authors: http://orcid.org/0000-0002-2009-8733
authors: SB
authors: Ryu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology*; D003433:Crosses, Genetic; D035264:Flowers / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000378:metabolism*; D005875:Gibberellins / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006368:Helianthus / Q000201:enzymology*; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology; D030821:Plants, Genetically Modified; D021381:Protein Transport; D012639:Seeds / Q000254:growth & development*; D013347:Subcellular Fractions / Q000201:enzymology; D031205:Taraxacum / Q000235:genetics / Q000254:growth & development; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development*; D019076:Transgenes
chemical_list: D005875:Gibberellins; D002734:Chlorophyll; D049452:Green Fluorescent Proteins; D002338:Carotenoids; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D005966:Glucuronidase
keywords: chloroplast; fast growth; geranylgeranyl pyrophosphate synthase; gibberellins; higher yield
references: Plant Physiol. 1994 Apr;104(4):1469-70
references: 8016276
references: Nature. 1993 Nov 4;366(6450):14-5
references: 8232528
references: Plant Mol Biol. 1995 Jan;27(2):425-8
references: 7888631
references: Plant J. 1998 Dec;16(6):735-43
references: 10069079
references: Plant J. 2000 May;22(3):247-56
references: 10849342
references: Plant J. 2014 Nov;80(3):462-74
references: 25146977
references: Plant Cell. 2011 Feb;23(2):628-40
references: 21325138
references: Biotechnol Lett. 2009 Feb;31(2):305-12
references: 18936881
references: Plant Sci. 2000 Apr 25;153(2):97-105
references: 10717315
references: Mol Biol Rep. 2012 Mar;39(3):3235-43
references: 21706164
references: J Exp Bot. 2007;58(6):1407-20
references: 17317673
references: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Oct;32(5):563-9
references: 17075180
references: Plant J. 1992 Jan;2(1):25-34
references: 1303794
references: Plant Cell. 2002;14 Suppl:S61-80
references: 12045270
references: Plant Physiol. 2000 Apr;122(4):1045-56
references: 10759500
references: Plant Physiol. 1998 Nov;118(3):773-81
references: 9808721
references: Plant Cell. 1991 May;3(5):445-459
references: 12324600
references: Plant Physiol. 2001 Dec;127(4):1682-93
references: 11743113
references: Plant Physiol. 1984 Jun;75(2):318-22
references: 16663618
references: BMC Plant Biol. 2004 Nov 18;4:18
references: 15550168
references: Plant Physiol. 1998 Mar;116(3):1003-11
references: 9501132
references: Plant Cell Physiol. 2010 Oct;51(10):1694-706
references: 20798276
references: Proc Natl Acad Sci U S A. 2004 May 18;101(20):7827-32
references: 15128937
references: Physiol Plant. 2001 Jun;112(2):251-260
references: 11454231
references: Plant Physiol. 1995 Jun;108(2):837-8
references: 7610177
references: J Food Sci. 2010 Jan-Feb;75(1):C55-61
references: 20492150
references: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W585-7
references: 17517783
references: Plant Physiol. 2004 May;135(1):254-65
references: 15122040
references: Plant Mol Biol. 1997 Oct;35(3):331-41
references: 9349257
references: Nat Biotechnol. 2000 Jun;18(6):666-9
references: 10835607
references: Arabidopsis Book. 2012;10:e0158
references: 22582030
references: Plant Mol Biol. 2013 Jul;82(4-5):393-416
references: 23729351