title: Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance.
abstract: Phospholipase C1 (PLC1), secretory phospholipase A2 (sPLA2) and Ca(2+)/H(+) exchanger proteins regulate calcium signaling and homeostasis in eukaryotes. In this study, we investigate functions for phospholipase C1 (plc-1), sPLA2 (splA2) and a Ca(2+)/H(+) exchanger (cpe-1) in the filamentous fungus Neurospora crassa. The Δplc-1, ΔsplA2, and Δcpe-1 mutants exhibited a growth defect on medium supplemented with the divalent ionophore A23187, suggesting that these genes might play a role in regulation of cytosolic free Ca(2+) concentration ([Ca(2+)](c)) in N. crassa. The strains lacking plc-1, splA2, and cpe-1 possessed higher carotenoid content than wild type at 8°C, 22°C, and 30°C, and showed increased ultraviolet (UV)-survival under conditions that induced carotenoid accumulation. Moreover, Δplc-1, ΔsplA2, and Δcpe-1 mutants showed reduced survival rate under hydrogen peroxide-induced oxidative stress and induced thermotolerance after exposure to heat shock temperatures. Thus, this study revealed multiple cellular roles for plc-1, splA2, and cpe-1 genes in regulation of [Ca(2+)](c), carotenoid accumulation, survival under stress conditions, and acquisition of thermotolerance induced by heat shock.
authors: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, India.
authors: Ananya
authors: A
authors: Barman
authors: Ranjan
authors: R
authors: Tamuli
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020013:Calcium Signaling; D002338:Carotenoids / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D018869:Heat-Shock Response; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D018384:Oxidative Stress; D054467:Phospholipases A2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D010738:Type C Phospholipases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D017920:Antiporters; D027682:Cation Transport Proteins; C027614:calcium-hydrogen antiporters; D002338:Carotenoids; D006861:Hydrogen Peroxide; D054467:Phospholipases A2; D010738:Type C Phospholipases; C432456:phospholipase C1
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