title: Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure.
abstract: mFISH analysis of chromosome aberration profiles of 47 and 144 h lymphocyte cultures following exposure to 193 mGy α-particle radiation confirmed that the frequency of stable aberrant cells and stable cells carrying translocations remains constant through repeated cell divisions. Age-specific rates and in vitro dose-response curves were used to derive expected translocation yields in nine workers from the Mayak nuclear facility in Russia. Five had external exposure to γ-radiation, two of whom also had exposure to neutrons, and four had external exposure to γ-radiation and internal exposure to α-particle radiation from incorporated plutonium. Doubts over the appropriateness of the dose response used to estimate translocations from the neutron component made interpretation difficult in two of the workers with external exposure, but the other three had translocation yields broadly in line with expectations. Three of the four plutonium workers had translocation yields in line with expectations, thus supporting the application of the recently derived in vitro α-particle dose response for translocations in stable cells. Overall this report demonstrates that with adequate reference in vitro dose-response curves, translocation yield has the potential to be a useful tool in the validation of red bone marrow doses resulting from mixed exposure to external and internal radiation.
authors: Centre for Integrated Genomic Medical Research (CIGMR), School of Population Health, Faculty of Medical and Human Sciences, The University of Manchester, Manchester, M13 9PT, UK.
authors: Gillian B
authors: GB
authors: Curwen
authors: Natalia V
authors: NV
authors: Sotnik
authors: Kevin K
authors: KK
authors: Cadwell
authors: Tamara V
authors: TV
authors: Azizova
authors: Mark A
authors: MA
authors: Hill
authors: E Janet
authors: EJ
authors: Tawn
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000512:Alpha Particles / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009688:Nuclear Reactors; D016273:Occupational Exposure / Q000009:adverse effects*; D011005:Plutonium / Q000009:adverse effects*; D000069079:Radiation Exposure / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D011005:Plutonium
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