title: Detection of Salivary Human Papilloma Viruses 16 and 18 (HPV) in Smoker Men in an Iranian Population by PCR: A Pilot Study.
abstract: BACKGROUND
  Human papilloma virus (HPV) infection of the oropharynx is acquired through a variety of sexual and social forms of transmission. Recent epidemiologic evidence has suggested that HPV may be an independent risk factor for oropharyngeal cancers, but risk factors for persistent HPV infection in the oropharynx are unknown. More evidence is needed regarding the prevalence of oral high-risk HPV among healthy smoker and nonsmoker adults.
  
  
  OBJECTIVE
  The aim of this study was to compare salivary infection with human papilloma virus types 16 and 18 between smokers and nonsmokers.
  
  
  METHODS
  A hundred healthy adult subjects were selected from Zahedan dental school for this pilot study. DNA was isolated from saliva samples and screened for high-risk HPV strains of HPV 16 and 18. Then, further processed using Real Time PCR for quantification and confirmation of sensitivity and specificity of the test. Data was analyzed by t-test.
  
  
  RESULTS
  There were no high-risk types of virus in patients and no significant differences between the groups (P = 1).
  
  
  CONCLUSIONS
  It seems that smoking cannot increase the prevalence of high risk HPV 16, 18 in saliva samples.
authors: Genetics of Non Communicable Disease Research Center, Department of Oral Medicine, Zahedan University of Medical Sciences.
authors: Fateme
authors: F
authors: Arbabi-Kalati
authors: Dental Research center, Department of Oral Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran.
authors: Tahereh
authors: T
authors: Nosratzehi
authors: Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran.
authors: Zakaria
authors: Z
authors: Bameri
authors: Department of Dentistry, Zahedan University of Medical Sciences, Zahedan, IR Iran.
authors: Fiz Mohammad
authors: FM
authors: Rigi
keywords: DNA Probes, HPV; Saliva; Smoking
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