title: Protective Effects of a Novel Probiotic Strain of Lactobacillus plantarum JSA22 from Traditional Fermented Soybean Food Against Infection by Salmonella enterica Serovar Typhimurium.
abstract: Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biological preservatives in the food and agricultural industries. However, the molecular mechanisms used by lactobacilli to suppress pathogenic bacterial infections have been poorly characterized. We previously isolated Lactobacillus plantarum JSA22 from buckwheat sokseongjang, a traditional Korean fermented soybean food, which possessed high enzymatic, fibrinolytic, and broad-spectrum antimicrobial activity against foodborne pathogens. In this study, we investigated the effects of L. plantarum JSA22 on the growth of S. Typhimurium and S. Typhimurium-induced cytotoxicity by stimulating the host immune response in intestinal epithelial cells. The results showed that coincubation of S. Typhimurium and L. plantarum JSA22 with intestinal epithelial cells suppressed S. Typhimurium infection, S. Typhimurium-induced NF-kappaB activation, and IL-8 production, and lowered the phosphorylation of both Akt and p38. These data indicated that L. plantarum JSA22 has probiotic properties, and can inhibit S. Typhimurium infection of intestinal epithelial cells. Our findings can be used to develop therapeutic and prophylactic agents against pathogenic bacteria.
authors: Department of Agro-food Resources, National Academy of Agricultural Science, Jeollabuk-do 565-851, Republic of Korea.
authors: Jeong Seon
authors: JS
authors: Eom
authors: Department of Agro-food Resources, National Academy of Agricultural Science, Jeollabuk-do 565-851, Republic of Korea.
authors: Jin
authors: J
authors: Song
authors: Department of Agro-food Resources, National Academy of Agricultural Science, Jeollabuk-do 565-851, Republic of Korea.
authors: Hye Sun
authors: HS
authors: Choi
mesh_terms: D000898:Antibiosis; D002460:Cell Line; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D050296:Microbial Viability; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D019936:Probiotics / Q000008:administration & dosage*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012480:Salmonella Infections / Q000378:metabolism / Q000382:microbiology* / Q000517:prevention & control*; D012486:Salmonella typhimurium; D045730:Soy Foods / Q000382:microbiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D016328:NF-kappa B; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases