title: Transcriptome of the human retina, retinal pigmented epithelium and choroid.
abstract: The retina and its adjacent supporting tissues - retinal pigmented epithelium (RPE) and choroid - are critical structures in human eyes required for normal visual perception. Abnormal changes in these layers have been implicated in diseases such as age-related macular degeneration and glaucoma. With the advent of high-throughput methods, such as serial analysis of gene expression, cDNA microarray, and RNA sequencing, there is unprecedented opportunity to facilitate our understanding of the normal retina, RPE, and choroid. This information can be used to identify dysfunction in age-related macular degeneration and glaucoma. In this review, we describe the current status in our understanding of these transcriptomes through the use of high-throughput techniques.
authors: Center for Applied Genomics, The Children's Hospital of Philadelphia, Philadelphia, Pa 19104, USA. Electronic address: tianl@email.chop.edu.
authors: Lifeng
authors: L
authors: Tian
authors: Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pa 19104, USA. Electronic address: kristakazmar@gmail.com.
authors: Krista L
authors: KL
authors: Kazmierkiewicz
authors: Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pa 19104, USA. Electronic address: anitabow@mail.med.upenn.edu.
authors: Anita S
authors: AS
authors: Bowman
authors: Department of Biostatistics and Epidemiology, University of Pennsylvania, Philadelphia, Pa 19104, USA. Electronic address: mingyao@mail.med.upenn.edu.
authors: Mingyao
authors: M
authors: Li
authors: Department of Ophthalmology, University of Alabama School of Medicine, Birmingham, Al 35294, USA. Electronic address: curcio@uab.edu.
authors: Christine A
authors: CA
authors: Curcio
authors: Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pa 19104, USA. Electronic address: stamboli@mail.med.upenn.edu.
authors: Dwight E
authors: DE
authors: Stambolian
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002829:Choroid / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D010858:Pigmentation; D012326:RNA Splicing; D012160:Retina / Q000378:metabolism*; D059467:Transcriptome
keywords: Age-related macular degeneration; Choroid; Glaucoma; RNA-Seq; Retina; Retinal pigmented epithelium; Serial analysis of gene expression; Transcriptome; cDNA microarray
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