title: Enzymatic cleavage of histone H3: a new consideration when measuring histone modifications in human samples.
abstract: Histone modifications are increasingly being used as biomarkers of cancer prognosis and survival. However, we identified a cleavage product of histone H3 in human peripheral blood mononuclear cells, which interferes with measures of certain H3 modifications. Therefore, the potential for enzymatic cleavage of histones should be considered when measuring histone modifications in human samples. Furthermore, the enzymatic cleavage of human H3 is itself a fascinating area of research and two important questions remain to be answered: 1) Does cleavage of human H3 occur in vivo, as it does in other organisms? and 2) Does it serve a biologically important function?
authors: Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 650 W. 168th Street, Room 1618, New York, NY 10032 USA.
authors: Caitlin G
authors: CG
authors: Howe
authors: Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 W. 168th Street, Room 1107E, New York, NY 10032 USA.
authors: Mary V
authors: MV
authors: Gamble
keywords: Enzymatic cleavage; Histone H3; Histone modifications
references: FASEB J. 2002 Mar;16(3):429-31
references: 11821259
references: Biochim Biophys Acta. 2003 Mar 21;1646(1-2):207-15
references: 12637028
references: Biochem Biophys Res Commun. 2003 Dec 26;312(4):1082-6
references: 14651982
references: Arch Gerontol Geriatr. 2000 Mar-Apr;30(2):109-14
references: 15374037
references: Nature. 2004 Nov 18;432(7015):406-11
references: 15525939
references: Antimicrob Agents Chemother. 2005 Jun;49(6):2399-406
references: 15917539
references: Cell. 2008 Oct 17;135(2):284-94
references: 18957203
references: Cell. 2008 Nov 14;135(4):604-7
references: 19013272
references: Nat Struct Mol Biol. 2009 Jan;16(1):17-22
references: 19079264
references: Fish Shellfish Immunol. 2009 Nov;27(5):639-46
references: 19706329
references: J Virol. 1990 Feb;64(2):748-56
references: 2153239
references: Virology. 1990 Feb;174(2):364-74
references: 2154880
references: Environ Health Perspect. 2012 Feb;120(2):198-203
references: 22024396
references: Biochem Biophys Res Commun. 2012 May 4;421(2):261-7
references: 22503985
references: Am J Cancer Res. 2012;2(5):589-97
references: 22957310
references: Cancer Epidemiol Biomarkers Prev. 2013 Jun;22(6):1052-60
references: 23629520
references: Mol Biol Rep. 2014 May;41(5):2717-30
references: 24469733
references: PLoS One. 2014 Feb 28;9(2):e90496
references: 24587380
references: Stem Cell Res. 2014 Jul;13(1):123-34
references: 24874291
references: PLoS One. 2014 Jul 23;9(7):e103230
references: 25054204
references: Curr Mol Med. 2014;14(9):1164-72
references: 25323999
references: Bioessays. 2015 Jan;37(1):70-9
references: 25350939
references: ISRN Mol Biol. 2012 Oct 15;2012:219656
references: 27335663
references: ISRN Mol Biol. 2013 Mar 03;2013:185807
references: 27335674
references: ISRN Mol Biol. 2013 Jun 02;2013:930216
references: 27398241
references: Cell. 1980 May;20(1):55-64
references: 6993010
references: Eur J Biochem. 1996 Apr 1;237(1):86-92
references: 8620898
references: Infect Immun. 1998 Jul;66(7):3255-63
references: 9632593
references: FEBS Lett. 1998 Oct 23;437(3):258-62
references: 9824303