title: Angular-type furocoumarins from the roots of Angelica atropurpurea and their inhibitory activity on the NFAT signal transduction pathway.
abstract: One new (1) and two known angular-type (2,3) furocoumarins, isoarchangelicin (1), archangelicin (2), and 2'-angeloyl-3'-isovaleryl vaginate (3), were isolated from the roots of Angelica atropurpurea. The structure of the new compound was established on the basis of one- and two-dimensional NMR spectra and other spectroscopic studies. The inhibitory activity of these three compounds and a deacylated form of archangelicin (4) on the nuclear factor of activated T cells (NFAT) signal transduction pathway was tested by NFAT-responsive luciferase reporter gene assay in cultured cells. Although 4 did not exhibit inhibitory activity on NFAT signaling, 1-3 exhibited dose-dependent inhibition with IC50 values of 16.5 (1), 9.0 (2), and 9.2 (3) μM.
authors: Azumi
authors: A
authors: Nagasawa
authors: Mitsuyoshi
authors: M
authors: Sakasai
authors: Daishi
authors: D
authors: Sakaguchi
authors: Shigeru
authors: S
authors: Moriwaki
authors: Yoshinori
authors: Y
authors: Nishizawa
authors: Takashi
authors: T
authors: Kitahara
mesh_terms: D029969:Angelica / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D011564:Furocoumarins / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D050778:NFATC Transcription Factors / Q000037:antagonists & inhibitors*; D018517:Plant Roots / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D011564:Furocoumarins; D050778:NFATC Transcription Factors