title: Spatial mapping of proteoglycan content in articular cartilage using near-infrared (NIR) spectroscopy.
abstract: Diagnosis of articular cartilage pathology in the early disease stages using current clinical diagnostic imaging modalities is challenging, particularly because there is often no visible change in the tissue surface and matrix content, such as proteoglycans (PG). In this study, we propose the use of near infrared (NIR) spectroscopy to spatially map PG content in articular cartilage. The relationship between NIR spectra and reference data (PG content) obtained from histology of normal and artificially induced PG-depleted cartilage samples was investigated using principal component (PC) and partial least squares (PLS) regression analyses. Significant correlation was obtained between both data (R(2) = 91.40%, p<0.0001). The resulting correlation was used to predict PG content from spectra acquired from whole joint sample, this was then employed to spatially map this component of cartilage across the intact sample. We conclude that NIR spectroscopy is a feasible tool for evaluating cartilage contents and mapping their distribution across mammalian joint.
authors: Department of Applied Physics, University of Eastern Finland, Kuopio, Finland ; School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
authors: Isaac O
authors: IO
authors: Afara
authors: School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
authors: Hayley
authors: H
authors: Moody
authors: Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.
authors: Sanjleena
authors: S
authors: Singh
authors: School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia.
authors: Indira
authors: I
authors: Prasadam
authors: School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, Australia ; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
authors: Adekunle
authors: A
authors: Oloyede
keywords: (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization
references: J Bone Joint Surg Br. 2002 Jan;84(1):42-7
references: 11837831
references: Clin Biomech (Bristol, Avon). 2007 Aug;22(7):843-8
references: 17573168
references: Osteoarthritis Cartilage. 2002 Jun;10(6):432-63
references: 12056848
references: Histochemistry. 1985;82(3):249-55
references: 2581923
references: Biochim Biophys Acta. 2006 Jul;1758(7):934-41
references: 16815242
references: Eur Radiol. 2013 Feb;23(2):496-504
references: 22886535
references: Am J Sports Med. 2010 Dec;38(12):2516-21
references: 20847221
references: Biomed Opt Express. 2012 Sep 1;3(9):2288-98
references: 23024920
references: Arch Orthop Trauma Surg. 2011 Mar;131(3):377-81
references: 21249375
references: J Bone Joint Surg Am. 1971 Jan;53(1):69-82
references: 4250366
references: Osteoarthritis Cartilage. 2012 Nov;20(11):1367-73
references: 22820498
references: Histochem J. 1996 Aug;28(8):577-90
references: 8894661
references: Med Eng Phys. 2008 Apr;30(3):285-92
references: 17553725
references: Clin Biomech (Bristol, Avon). 2010 May;25(4):332-40
references: 20096492
references: J Orthop Res. 1994 Jul;12(4):474-84
references: 8064478
references: Arthroscopy. 2014 Sep;30(9):1146-55
references: 24951136
references: Clin Phys Physiol Meas. 1991 Nov;12(4):353-8
references: 1778034
references: Osteoarthritis Cartilage. 2010 Jan;18(1):73-81
references: 19733642
references: Osteoarthritis Cartilage. 2000 Jul;8(4):288-93
references: 10903883
references: J Anat. 2006 Aug;209(2):259-67
references: 16879604
references: Microsc Res Tech. 2010 May;73(5):503-12
references: 19839035
references: Instr Course Lect. 1998;47:487-504
references: 9571450
references: J Mech Behav Biomed Mater. 2013 Apr;20:249-58
references: 23384759
references: Magn Reson Med. 2003 Mar;49(3):488-92
references: 12594751
references: BMC Musculoskelet Disord. 2007 May 29;8:47
references: 17535439
references: Bone. 2013 Apr;53(2):350-7
references: 23274676
references: Med Eng Phys. 2013 Jan;35(1):88-95
references: 22824725