title: The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1.
abstract: We have shown that Mg/EGTA (5 mM Mg(2+) and 1.5 mM EGTA) could effectively promote the adhesion of integrin αLβ2 to its ligand ICAM-1 but could not promote that of the αMβ2 to denatured BSA. In order to determine the structural differences between αL and αM that specifically contribute to Mg/EGTA sensitivity, a series of αL/αM chimeras were constructed. Our results showed that αLβ2 with αM calf-1 domain completely lost the response to Mg/EGTA activation. In the reverse experiment, αMβ2 would require the presence of both the αL calf-1 and calf-2 domain to initiate the Mg/EGTA sensitivity.
authors: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors: Siyu
authors: S
authors: Guan
authors: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors: Ming
authors: M
authors: Cheng
authors: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. Electronic address: alaw@ntu.edu.sg.
authors: S K Alex
authors: SK
authors: Law
mesh_terms: D001665:Binding Sites; D002448:Cell Adhesion / Q000502:physiology*; D004533:Egtazic Acid / Q000737:chemistry* / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000737:chemistry* / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D013329:Structure-Activity Relationship
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D004533:Egtazic Acid; D008274:Magnesium
keywords: Cell adhesion; Divalent cation; Domain swapping; Integrin activation