title: Effects of infusion of human methemoglobin solution following hydrogen sulfide poisoning.
abstract: BACKGROUND
  We have recently reported that infusion of a solution containing methemoglobin (MetHb) during exposure to hydrogen sulfide results in a rapid and large decrease in the concentration of the pool of soluble/diffusible H2S in the blood. However, since the pool of dissolved H2S disappears very quickly after H2S exposure, it is unclear if the ability of MetHb to "trap" sulfide in the blood has any clinical interest and relevance in the treatment of sulfide poisoning.
  
  
  METHODS
  In anesthetized rats, repetition of short bouts of high level of H2S infusions was applied to allow the rapid development of an oxygen deficit. A solution containing MetHb (600 mg/kg) or its vehicle was administered 1 min and a half after the end of H2S intoxication.
  
  
  RESULTS
  The injection of MetHb solution increased methemoglobinemia to about 6%, almost instantly, but was unable to affect the blood concentration of soluble H2S, which had already vanished at the time of infusion, or to increase combined H2S. In addition, H2S-induced O2 deficit and lactate production as well as the recovery of carotid blood flow and blood pressure were similar in treated and control animals.
  
  
  CONCLUSIONS
  Our results do not support the view that administration of MetHb or drugs-induced methemoglobinemia during the recovery phase following severe H2S intoxication in sedated rats can restore cellular oxidative metabolism, as the pool of diffusible sulfide, accessible to MetHb, disappears rapidly from the blood after H2S exposure.
authors: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Pennsylvania State University College of Medicine , Hershey, PA , USA.
authors: B
authors: B
authors: Chenuel
authors: T
authors: T
authors: Sonobe
authors: P
authors: P
authors: Haouzi
mesh_terms: D000818:Animals; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D006862:Hydrogen Sulfide / Q000097:blood / Q000493:pharmacokinetics / Q000506:poisoning*; D007262:Infusions, Intravenous; D019344:Lactic Acid / Q000097:blood; D008706:Methemoglobin / Q000008:administration & dosage / Q000627:therapeutic use*; D010101:Oxygen Consumption / Q000187:drug effects; D019999:Pharmaceutical Solutions; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019999:Pharmaceutical Solutions; D019344:Lactic Acid; D008706:Methemoglobin; D006862:Hydrogen Sulfide
keywords: H2S toxicity; Metallo-compound; Methemoglobin; Rat model
references: Antioxid Redox Signal. 2012 Jul 1;17(1):32-44
references: 22074253
references: Respir Physiol Neurobiol. 2011 Aug 15;177(3):273-83
references: 21569867
references: Antioxid Redox Signal. 2013 Aug 10;19(5):510-6
references: 22233239
references: Am J Physiol Regul Integr Comp Physiol. 2013 Sep 15;305(6):R630-8
references: 23904109
references: Clin Toxicol (Phila). 2014 Jun;52(5):566
references: 24895940
references: Crit Care. 2012;16(5):R178
references: 23031430
references: Toxicol Sci. 2014 Oct;141(2):493-504
references: 25015662
references: Toxicol Sci. 2002 Jan;65(1):18-25
references: 11752681
references: Drug Metab Dispos. 2002 Jun;30(6):676-83
references: 12019195
references: Toxicol Appl Pharmacol. 1966 Jan;8(1):159-72
references: 5921892
references: Mol Pharmacol. 1967 Jul;3(4):378-85
references: 6033636
references: Toxicol Appl Pharmacol. 1969 Nov;15(3):505-16
references: 5353816
references: Arch Environ Health. 1976 May-Jun;31(3):166-9
references: 1275562
references: J Occup Med. 1979 Feb;21(2):93-7
references: 556262
references: Ann Intern Med. 1981 Dec;95(6):782
references: 7305166
references: Am Ind Hyg Assoc J. 1981 Nov;42(11):805-9
references: 7315740
references: Blood. 1984 Feb;63(2):335-41
references: 6692038
references: Am J Ind Med. 1991;20(1):91-101
references: 1867221
references: Hum Exp Toxicol. 1996 Jan;15(1):19-25
references: 8845204
references: Occup Med (Lond). 1996 Oct;46(5):367-71
references: 8918153
references: Vet Hum Toxicol. 1997 Jun;39(3):152-4
references: 9167244
references: J Biol Chem. 1998 May 15;273(20):12128-34
references: 9575158
references: Biochim Biophys Acta. 2005 Sep 15;1725(2):201-12
references: 15996823
references: J Hypertens. 2007 Mar;25(3):599-608
references: 17278977
references: Toxicology. 2007 Dec 5;242(1-3):16-22
references: 17976885
references: Respir Physiol Neurobiol. 2008 Jan 1;160(1):109-15
references: 17980679
references: Biochim Biophys Acta. 2008 Oct;1784(10):1378-81
references: 18206989
references: J Bioenerg Biomembr. 2008 Oct;40(5):533-9
references: 18839291
references: Respir Physiol Neurobiol. 2009 Jul 31;167(3):316-22
references: 19539788
references: Basic Clin Pharmacol Toxicol. 2009 Nov;105(5):294-300
references: 19671050
references: Sci Signal. 2009;2(96):ra72
references: 19903941
references: Br J Pharmacol. 2010 Jun;160(4):941-57
references: 20590590
references: Respir Physiol Neurobiol. 2013 Mar 1;186(1):87-94
references: 23333818