title: Purification and characterization of chitinases from ridgetail white prawn Exopalaemon carinicauda.
abstract: In this paper, we purified two native chitinases from the hepatopancreas of the ridgetail white prawn Exopalaemon carinicauda by using ion-exchange resin chromatography (IEC) and gel filtration. These two chitinases, named EcChi1 and EcChi2, were identified by chitinolytic activity assay and LC-ESI-MS/MS. Their apparent molecular weights were 44 kDa and 65 kDa as determined by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity of EcChi1 and EcChi2 was 1305.97 U·mg-1 and 28.69 U·mg-1. The optimal temperature and pH of EcChi1 were 37 °C and pH 4.0, respectively. Co2+, Fe3+, Zn2+, Cd2+, and Cu2+ had an obvious promoting effect upon chitinase activity of EcChi1. For colloidal chitin, the Km and Vmax values of EcChi1 were 2.09 mg·mL-1 and 31.15 U·mL-1·h-1.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. wangjing926453@gmail.com.
authors: Jing
authors: J
authors: Wang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. zhangjiquan@qdio.ac.cn.
authors: Jiquan
authors: J
authors: Zhang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. xiangyangge2@163.com.
authors: Fengge
authors: F
authors: Song
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. guitianshu@hust.edu.cn.
authors: Tianshu
authors: T
authors: Gui
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. jhxiang@qdio.ac.cn.
authors: Jianhai
authors: J
authors: Xiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000302:isolation & purification*; D001483:Base Sequence; D055162:Biocatalysis; D002686:Chitin / Q000737:chemistry; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033541:Palaemonidae / Q000201:enzymology*; D017422:Sequence Analysis, DNA
chemical_list: D060829:Arthropod Proteins; D002686:Chitin; D002688:Chitinases
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