title: [Cytotoxicity of a novel endodontic treatment material iRoot BP Plus to human gingival fibroblasts].
abstract: OBJECTIVE
  To compare the cytotoxicity of a novel endodontic treatment material iRoot BP Plus and mineral trioxide aggregate (MTA) to human gingival fibroblasts in vitro.
  
  
  METHODS
  Cultured human gingival fibroblasts were exposed to multiple concentrations of material elutes (no dilution, 1:2 dilution, and 1:5 dilution). The test material samples were immersed and incubated in the culture medium for 1, 3 or 7 days at 37 degrees centigrade. The proliferation rate was evaluated using methylthiazol tetrazolium (MTT) assay. Cell relative growth rates were presented as x±s. The data was statistically analyzed by factorial design ANOVA using SPSS 20.0 software package.
  
  
  RESULTS
  Cell relative growth rates of the eluates of iRoot BP Plus and MTA in different concentrations ranged from 77.31% to 113.82%. The cytotoxicity grade of both materials was 0 or 1 (no cytotoxicity). There was no significant difference in the relative growth rate in different concentrations of iRoot BP plus and MTA eluates under different elution times (F(concentration×time×material)=1.393, P=0.256).
  
  
  CONCLUSIONS
  Both iRoot BP Plus and MTA exhibit minimal level of cytotoxicity.
authors: Department of Pediatric Dentistry, School of Stomatology, China Medical University,Liaoning Research Institute of Stomatology. Shenyang 110002, Liaoning Province, China.E-mail:shuangshi@hotmail.com.
authors: Shuang
authors: S
authors: Shi
authors: Zhi-fan
authors: ZF
authors: Bao
authors: Xu
authors: X
authors: Chen
authors: Dan-dan
authors: DD
authors: Zhang
mesh_terms: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002470:Cell Survival; D004338:Drug Combinations; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D010087:Oxides; D017640:Silicates; D013778:Tetrazolium Salts
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; D013778:Tetrazolium Salts; C086631:mineral trioxide aggregate