title: Oral immunization of mice with recombinant rabies vaccine strain (ERAG3G) induces complete protection.
abstract: OBJECTIVE
  New rabies vaccine bait for both pets and raccoon dogs residing in Korea is needed to eradicate rabies infection among animals. In this study, we constructed a recombinant rabies virus (RABV), the ERAG3G strain, using a reverse genetics system. Then we investigated the efficacy of this strain in mice after oral administration and the safety of this strain in cats after intramuscular administration.
  
  
  METHODS
  The ERAG3G strain was rescued in BHK/T7-9 cells using the full-length genome mutated at the amino acid position 333 of the glycoprotein gene of RABV and helper plasmids. Four-week-old mice underwent one or two oral administrations of the ERAG3G strain and were challenged with the highly virulent RABV strain CVSN2c 14 days after the second administration. Clinical symptoms were observed and body weights were measured every day after the challenge.
  
  
  RESULTS
  All mice showed complete protection against virulent RABV. In addition, cats intramuscularly inoculated with the ERAG3G strain showed high antibody titers ranging from 2.62 to 23.9 IU/mL at 28-day postinoculation.
  
  
  CONCLUSIONS
  The oral immunization of the ERAG3G strain plays an important role in conferring complete protection in mice, and intramuscular inoculation of the ERAG3G strain induces the formation of anti-rabies neutralizing antibody in cats.
authors: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors: Dong-Kun
authors: 0000-0001-5765-3043
authors: DK
authors: Yang
authors: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors: Ha-Hyun
authors: 0000-0001-6473-0035
authors: HH
authors: Kim
authors: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors: Sung-Suk
authors: 0000-0001-5124-9736
authors: SS
authors: Choi
authors: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors: Jong-Taek
authors: 0000-0002-6388-550X
authors: JT
authors: Kim
authors: Gangwon-do Veterinary Service Laboratory, Chuncheon, Korea.
authors: Woong-Ho
authors: 0000-0003-1776-8297
authors: WH
authors: Jeong
authors: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors: Jae-Young
authors: 0000-0002-9554-9252
authors: JY
authors: Song
keywords: Animals; Mouse; Recombinant rabies virus; Reverse genetics
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