title: A ribosomal protein AgRPS3aE from halophilic Aspergillus glaucus confers salt tolerance in heterologous organisms.
abstract: High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops.
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. xlliang09@mails.jlu.edu.cn.
authors: Xilong
authors: X
authors: Liang
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. liuyiling21th@126.com.
authors: Yiling
authors: Y
authors: Liu
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. lixiaxie82@126.com.
authors: Lixia
authors: L
authors: Xie
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. xlliang09@mails.jlu.edu.cn.
authors: Xiaodan
authors: X
authors: Liu
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. wyziyu@163.com.
authors: Yi
authors: Y
authors: Wei
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. yangxiaoZ897@163.com.
authors: Xiaoyang
authors: X
authors: Zhou
authors: College of Plant Sciences, Jilin University, Changchun 130062, China. zhang_sh@jlu.edu.cn.
authors: Shihong
authors: S
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism; D001230:Aspergillus / Q000378:metabolism*; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000378:metabolism; D012269:Ribosomal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance; D036226:Seedlings / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D012269:Ribosomal Proteins
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