title: Comparative analysis of glycoprotein B (gB) of equine herpesvirus type 1 and type 4 (EHV-1 and EHV-4) in cellular tropism and cell-to-cell transmission.
abstract: Glycoprotein B (gB) plays an important role in alphaherpesvirus cellular entry and acts in concert with gD and the gH/gL complex. To evaluate whether functional differences exist between gB1 and gB4, the corresponding genes were exchanged between the two viruses. The gB4-containing-EHV-1 (EHV-1_gB4) recombinant virus was analyzed for growth in culture, cell tropism, and cell entry rivaling no significant differences when compared to parental virus. We also disrupted a potential integrin-binding motif, which did not affect the function of gB in culture. In contrast, a significant reduction of plaque sizes and growth kinetics of gB1-containing-EHV-4 (EHV-4_gB1) was evident when compared to parental EHV-4 and revertant viruses. The reduction in virus growth may be attributable to the loss of functional interaction between gB and the other envelope proteins involved in virus entry, including gD and gH/gL. Alternatively, gB4 might have an additional function, required for EHV-4 replication, which is not fulfilled by gB1. In conclusion, our results show that the exchange of gB between EHV-1 and EHV-4 is possible, but results in a significant attenuation of virus growth in the case of EHV-4_gB1. The generation of stable recombinant viruses is a valuable tool to address viral entry in a comparative fashion and investigate this aspect of virus replication further.
authors: Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, Berlin 14163, Germany. bart.spiesschaert@fu-berlin.de.
authors: Bart
authors: B
authors: Spiesschaert
authors: Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, Berlin 14163, Germany. no.34@fu-berlin.de.
authors: Nikolaus
authors: N
authors: Osterrieder
authors: Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, Berlin 14163, Germany. wfazab@zedat.fu-berlin.de.|Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt. wfazab@zedat.fu-berlin.de.
authors: Walid
authors: W
authors: Azab
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004285:Dogs; D015870:Gene Expression; D016679:Genome, Viral; D006566:Herpesviridae Infections / Q000662:veterinary; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D023141:Herpesvirus 4, Equid / Q000502:physiology*; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D006801:Humans; D016023:Integrins / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D056189:Viral Tropism; D053586:Virus Internalization; D014779:Virus Replication / Q000235:genetics
chemical_list: D016023:Integrins; D014759:Viral Envelope Proteins
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