title: Effect of cyclosporine on liver regeneration in partial hepatectomized rats.
abstract: OBJECTIVE
  To evaluate the influence of the cyclosporine in liver regeneration in rats submitted to an experimental model of 70% hepatectomy.
  
  
  METHODS
  Forty male rats were randomly divided in four subgroups (C.24h, C.7d, E.24h, E.7d), according to the drug used and the day of sacrifice (24 hours and 7 days). Cyclosporine (10 mg/Kg/day) was given to the study subgroup and 1 ml of 0.9% sodium chloride was to the control subgroup. Resection of left lateral lobe and median lobe performing 70% of liver mass. During the animals' death, KWON formula was applied. Counting of mitotic figures and percentage of positive nucleus with PCNA and Ki-67 were evaluated.
  
  
  RESULTS
  In the 2nd, 4th PO and death days, E.7d lose more weight than C.7d. Regarding to the KWON formula, the C.7d regenerated more than the C.24h and the same with the E.7d. Comparing between the groups, only E7d subgroup was statistically significant compared with C.7d, showing the stimulating effect of cyclosporine in liver regeneration. Immunohistochemestry had significant results between the study subgroups. The mitotic index revealed statistical differences in the control subgroups.
  
  
  CONCLUSIONS
  Cyclosporine, in spite of being an immunosuppressive drug, has a positive effect in liver regeneration, although reduce the animal's body weight.
authors: Department of Surgery and Liver Transplantation, Federal University of Parana, Curitiba, PR, Brazil.
authors: Giorgio Alfredo Pedroso
authors: GA
authors: Baretta
authors: Department of Surgery and Liver Transplantation, UFPR, Curitiba, PR, Brazil.
authors: Ozimo
authors: O
authors: Gama Filho
authors: Department of Surgery and Liver Transplantation, UFPR, Curitiba, PR, Brazil.
authors: Edimar Leandro
authors: EL
authors: Toderke
authors: Department of Surgery and Liver Transplantation, Federal University of Parana, Curitiba, PR, Brazil.
authors: André Ricardo Dall'Oglio
authors: AR
authors: Tolazzi
authors: Department of Surgery and Liver Transplantation, UFPR, Curitiba, PR, Brazil.
authors: Jorge Eduardo Fouto
authors: JE
authors: Matias
mesh_terms: D000818:Animals; D001835:Body Weight; D002452:Cell Count; D049109:Cell Proliferation; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D006498:Hepatectomy / Q000379:methods*; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008940:Mitotic Index; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D007166:Immunosuppressive Agents; D018809:Proliferating Cell Nuclear Antigen; D016572:Cyclosporine