title: Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity.
abstract: BACKGROUND
  Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture.
  
  
  RESULTS
  Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity.
  
  
  CONCLUSIONS
  We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production.
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan ; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075 Japan.
authors: Masaki
authors: M
authors: Muto
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan.
authors: Masayoshi
authors: M
authors: Tanaka
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan.
authors: Yue
authors: Y
authors: Liang
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan.
authors: Tomoko
authors: T
authors: Yoshino
authors: JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075 Japan ; Biotechnology Laboratory, Electric Power Development Co., Ltd., Yanagisaki-machi, Wakamatsu-ku, Kitakyusyu 808-0111 Japan.
authors: Mitsufumi
authors: M
authors: Matsumoto
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan ; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075 Japan.
authors: Tsuyoshi
authors: T
authors: Tanaka
keywords: Biodiesel fuel; Fistulifera solaris; Glycerol kinase; Metabolic engineering; Mixotrophic cultivation; Oleaginous microalgae
references: Appl Biochem Biotechnol. 2010 May;161(1-8):483-90
references: 19756412
references: Photosynth Res. 2011 Sep;109(1-3):223-9
references: 21290260
references: Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21265-9
references: 22123974
references: Science. 2001 Jun 15;292(5524):2073-5
references: 11408656
references: Metab Eng. 2011 Jan;13(1):89-95
references: 20971205
references: Biochem J. 1985 Sep 1;230(2):379-88
references: 4052051
references: Biotechnol Bioeng. 2010 Oct 1;107(2):258-68
references: 20506159
references: Planta. 2004 Sep;219(5):827-35
references: 15107995
references: Science. 2004 Oct 1;306(5693):79-86
references: 15459382
references: Bioresour Technol. 2013 Jun;137:132-8
references: 23584413
references: Biotechnol Lett. 2009 Jul;31(7):1043-9
references: 19322523
references: Crit Rev Biotechnol. 2002;22(3):245-79
references: 12405558
references: Can J Microbiol. 1962 Apr;8:229-39
references: 13902807
references: Biotechnol Prog. 2011 Jan-Feb;27(1):54-60
references: 21312355
references: Mar Biotechnol (NY). 2013 Feb;15(1):48-55
references: 22555859
references: J Biosci Bioeng. 2014 Jun;117(6):720-4
references: 24388444
references: Nat Commun. 2012 Feb 21;3:686
references: 22353717
references: Plant Biotechnol J. 2007 Nov;5(6):802-14
references: 17764518
references: Biotechnol Bioeng. 2013 Jan;110(1):343-7
references: 22886471
references: Plant J. 2004 Feb;37(4):617-25
references: 14756771
references: Appl Microbiol Biotechnol. 2011 Aug;91(3):835-44
references: 21698379
references: Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19748-53
references: 24248374
references: Mol Gen Genet. 1996 Oct 16;252(5):572-9
references: 8914518
references: Nature. 2012 Aug 16;488(7411):329-35
references: 22895338