title: Identification of the interaction domains of white spot syndrome virus envelope proteins VP28 and VP24.
abstract: VP28 and VP24 are two major envelope proteins of white spot syndrome virus (WSSV). The direct interaction between VP28 and VP24 has been described in previous studies. In this study, we confirmed this interaction and mapped the interaction domains of VP28 and VP24 by constructing a series of deletion mutants. By co-immunoprecipitation, two VP28-binding domains of VP24 were located at amino acid residues 46-61 and 148-160, while VP24-binding domain of VP28 was located at amino acid residues 31-45. These binding domains were further corroborated by peptide blocking assay, in which synthetic peptides spanning the binding domains were able to inhibit VP28-VP24 interaction, whereas same-size control peptides from non-binging regions did not.
authors: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China; School of Life Science, Xiamen University, Xiamen 361005, PR China.
authors: Zaipeng
authors: Z
authors: Li
authors: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China.
authors: Weiyu
authors: W
authors: Chen
authors: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China.
authors: Limei
authors: L
authors: Xu
authors: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China. Electronic address: lifang_87@163.com.
authors: Fang
authors: F
authors: Li
authors: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China. Electronic address: yangfeng5274@163.com.
authors: Feng
authors: F
authors: Yang
mesh_terms: D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins
keywords: Interaction domains; VP24; VP28; WSSV