title: Identification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12.
abstract: Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.
authors: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101, Japan, hmori@gtc.naist.jp.
authors: Hirotada
authors: H
authors: Mori
authors: Tomoya
authors: T
authors: Baba
authors: Katsushi
authors: K
authors: Yokoyama
authors: Rikiya
authors: R
authors: Takeuchi
authors: Wataru
authors: W
authors: Nomura
authors: Kazuichi
authors: K
authors: Makishi
authors: Yuta
authors: Y
authors: Otsuka
authors: Hitomi
authors: H
authors: Dose
authors: Barry L
authors: BL
authors: Wanner
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003227:Conjugation, Genetic; D017931:DNA Primers / Q000378:metabolism; D018274:Electroporation; D048168:Escherichia coli K12 / Q000235:genetics*; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D020043:Genes, Essential; D005804:Genes, Lethal; D005813:Genes, Synthetic; D005816:Genetic Complementation Test; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers