title: Interleukin-23 facilitates Th1 and Th2 cell differentiation in vitro following respiratory syncytial virus infection.
abstract: Respiratory syncytial virus (RSV) infection induces activation and imbalance of the immune system; however, the role of T helper 17 cells (Th17) in the response to RSV infection remains unclear. Interleukin-23 (IL-23) is a key cytokine in Th17 cell differentiation. The aim of this study was to explore the function of IL-23 in determining the distribution of Th lymphocyte subsets (Th1, Th2, and Th17) after RSV infection in vitro. Human bronchial epithelial cell line BEAS-2B was infected with mock or RSV at various multiplicities of infection (MOI) and transcript expression of IL-6, IL-23p19, and transforming growth factor (TGF-β) was detected by real-time polymerase chain reaction; IL-6, IL-23, and TGF-β in the supernatant were measured by enzyme-linked immunosorbent assay. The Th subset distribution in lymphocytes was determined by flow cytometry after co-culture with supernatants from mock and 72-hr RSV infection cultures. The role of IL-23 in lymphocytes was assessed by specific receptor blockade (IL-23R) prior to co-culture with supernatants from RSV-infected BEAS-2B cells, followed by flow cytometry to analyze Th subset differentiation. Cytokine expression increased after RSV infection. IL-23R blockade suppressed the differentiation of Th1, Th2, and Th17 cells in the presence of supernatants from RSV-infected BEAS-2B cells. RSV infection may induce cytokine secretion, thus inducing Th1, Th2, and Th17 differentiation via an IL-23R-dependent process.
authors: Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
authors: Jingjing
authors: J
authors: Feng
authors: Yunwen
authors: Y
authors: Hu
authors: Zhigang
authors: Z
authors: Song
authors: Yi
authors: Y
authors: Liu
authors: Xuejun
authors: X
authors: Guo
authors: Zhijun
authors: Z
authors: Jie
mesh_terms: D002460:Cell Line; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D053759:Interleukin-23 / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D053759:Interleukin-23
keywords: IL-23R; Th17 cells; human bronchial epithelial cells