title: Adhesion and activation of platelets from subjects with coronary artery disease and apparently healthy individuals on biomaterials.
abstract: On the basis of the clinical studies in patients with coronary artery disease (CAD) presenting an increased percentage of activated platelets, we hypothesized that hemocompatibility testing utilizing platelets from healthy individuals may result in an underestimation of the materials' thrombogenicity. Therefore, we investigated the interaction of polymer-based biomaterials with platelets from CAD patients in comparison to platelets from apparently healthy individuals. In vitro static thrombogenicity tests revealed that adherent platelet densities and total platelet covered areas were significantly increased for the low (polydimethylsiloxane, PDMS) and medium (Collagen) thrombogenic surfaces in the CAD group compared to the healthy subjects group. The area per single platelet-indicating the spreading and activation of the platelets-was markedly increased on PDMS treated with PRP from CAD subjects. This could not be observed for collagen or polytetrafluoroethylene (PTFE). For the latter material, platelet adhesion and surface coverage did not differ between the two groups. Irrespective of the substrate, the variability of these parameters was increased for CAD patients compared to healthy subjects. This indicates a higher reactivity of platelets from CAD patients compared to the healthy individuals. Our results revealed, for the first time, that utilizing platelets from apparently healthy donors bears the risk of underestimating the thrombogenicity of polymer-based biomaterials.
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors: S
authors: S
authors: Braune
authors: Department of Cardiology and Pneumology, Charitè-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.
authors: M
authors: M
authors: Groß
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors: M
authors: M
authors: Walter
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors: S
authors: S
authors: Zhou
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors: S
authors: S
authors: Dietze
authors: Department of Cardiology and Pneumology, Charitè-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.
authors: S
authors: S
authors: Rutschow
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors: A
authors: A
authors: Lendlein
authors: Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.|Department of Cardiology, Charitè-Universitätsmedizin Berlin, Campus Virchow, Berlin, Germany.
authors: C
authors: C
authors: Tschöpe
authors: Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Teltow and Berlin, Germany.
authors: F
authors: F
authors: Jung
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D003324:Coronary Artery Disease; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D010973:Platelet Adhesiveness; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012828:Silicones; C078211:polymethylsiloxane; D011138:Polytetrafluoroethylene
keywords: biomaterials; cardiovascular disease; cardiovascular implant; hemocompatibility; platelets