title: Imaging mitochondrial hydrogen peroxide in living cells.
abstract: Hydrogen peroxide (H2O2) produced from mitochondria is intimately involved in human health and disease but is challenging to selectively monitor inside living systems. The fluorescent probe MitoPY1 provides a practical tool for imaging mitochondrial H2O2 and has been demonstrated to function in a variety of diverse cell types. In this chapter, we describe the synthetic preparation of the small-molecule probe MitoPY1, methods for validating this probe in vitro and in live cells, and an example procedure for measuring mitochondrial H2O2 in a cell culture model of Parkinson's disease.
authors: Department of Chemistry and Center for Drug Discovery, Design, and Delivery, Southern Methodist University, Fondren Science Building, Office 141, 3215 Daniel Avenue, Dallas, TX, 75275, USA, alippert@smu.edu.
authors: Alexander R
authors: AR
authors: Lippert
authors: Bryan C
authors: BC
authors: Dickinson
authors: Elizabeth J
authors: EJ
authors: New
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D060326:Chemistry Techniques, Synthetic; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000138:chemical synthesis; D015203:Reproducibility of Results
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D006861:Hydrogen Peroxide