title: Influence of menstrual cycle on the expression of clock genes in peripheral blood mononuclear cells in Macaca fascicularis.
abstract: OBJECTIVE
  To investigate whether the expression patterns of periphery clock genes were influenced by menstrual cycle in a monkey model.
  
  
  METHODS
  In this preliminary study, the expression patterns of four clock genes (Bmal1, Clock, Cry1 and Per2) in peripheral blood mononuclear cells (PBMCs) from 6 female Macaca fascicularis in menstrual, late follicular and mid luteal phases of menstrual cycle were determined by qrt-PCR.
  
  
  RESULTS
  Bmal1 and Per2 mRNA levels were found to exhibit significant diurnal rhythms in all phases of the menstrual cycle. The expression of Cry1 mRNA was statistically rhythmic in late follicular and mid luteal phases. A main effect of menstrual cycle existed on the rhythms of Bmal1, Cry1 and Per2 expression, but not Clock expression. No significant differences were detected between menstrual phase and late follicular phase in all clock genes. Significant differences were found on the expression of Bmal1, Cry1 or Per2 mRNA between late follicular phase and mid luteal phase, when no difference existed in estrogen level, indicating the role of progesterone on biological clock gene expression. Furthermore, the peak of Bmal1 mRNA level slightly advanced in mid luteal phase compared with that in menstrual and late follicular phases.
  
  
  CONCLUSIONS
  The expression patterns of clock genes in PBMCs were influenced by menstrual cycle, potentially by the change of progesterone levels, and this effect maybe correlated with early pregnancy.
authors: Reproductive Medicine Center, Guangzhou Women and Children's Medical Center, Guangzhou, China; Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Jian
authors: J
authors: Xu
authors: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China. Electronic address: xuyanwen@live.cn.
authors: Yanwen
authors: Y
authors: Xu
authors: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Benyu
authors: B
authors: Miao
authors: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Mingfen
authors: M
authors: Deng
authors: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
authors: Yizi
authors: Y
authors: Wang
authors: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou, China.
authors: Peng
authors: P
authors: Xiang
authors: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou 510080, China; Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China. Electronic address: gzzhoucanquan@163.com.
authors: Canquan
authors: C
authors: Zhou
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics; D000818:Animals; D001683:Biological Clocks / Q000235:genetics*; D056926:CLOCK Proteins / Q000235:genetics; D004967:Estrogens / Q000097:blood*; D005260:Female; D015870:Gene Expression; D007963:Leukocytes, Mononuclear; D008252:Macaca fascicularis; D008597:Menstrual Cycle / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood*
chemical_list: D056930:ARNTL Transcription Factors; D004967:Estrogens; D012333:RNA, Messenger; D056926:CLOCK Proteins
keywords: Clock gene; Macaca fascicularis; Menstrual cycle; PBMCs