title: Real-time monitoring of H2O2 release from single cells using nanoporous gold microelectrodes decorated with platinum nanoparticles.
abstract: Here, we report a self-supported nanoporous gold microelectrode decorated with well-dispersed and tiny platinum nanoparticles as an electrochemical nonenzymatic hydrogen peroxide biosensor. Nanoporous gold was fabricated by electrochemical alloying/dealloying and then small-sized platinum nanoparticles were electrodeposited uniformly on them. This novel hybrid nanostructure endows the sensor with high sensitivity and selectivity towards the reduction of hydrogen peroxide with a low detection limit of 0.3 nM. The sensor has been successfully applied for the measurement of H2O2 release from a single isolated human breast cancer cell, demonstrating its great potential for further physiological and pathological applications.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. whhuang@whu.edu.cn.
authors: Chong
authors: C
authors: Xiao
authors: Yan-Ling
authors: YL
authors: Liu
authors: Jia-Quan
authors: JQ
authors: Xu
authors: Song-Wei
authors: SW
authors: Lv
authors: Shan
authors: S
authors: Guo
authors: Wei-Hua
authors: WH
authors: Huang
mesh_terms: D000497:Alloys / Q000737:chemistry; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D058608:Nanopores; D010316:Particle Size; D010984:Platinum / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D000497:Alloys; D010984:Platinum; D006046:Gold; D006861:Hydrogen Peroxide