title: Human beta-defensin-3 producing cells in septic implant loosening.
abstract: Human β-defensin-3 (hBD-3) has been found in synovial fluid and later in periprosthetic tissues in septic joint implant loosening. The aim of the present study was to identify its cellular sources. Tissue samples from 12 patients were analyzed. A fully automatic Leica BOND MAX staining robot was used. Affinity-purified rabbit anti-human hBD-3 IgG was applied in a two-layer horse radish peroxidase/anti-rabbit-labeled polymer method. Double immunofluorescence of hBD3 together with CD68, CD31, heat shock protein 47 (HSP47) and mast cell tryptase (MCT) staining was done. Human BD-3 was found in monocyte/macrophage-like cells, vascular endothelial cells and fibroblasts-like cells, but was weakly expressed in foreign body giant cells and negative in neutrophils. Human BD-3 was found in CD68 and CD31 immunoreactive cells, whereas HSP47 and MCT positive cells were hBD-3 negative. Immunostaining of hBD-3 was strong in some tissue areas but weak or absent in others. Monocyte/macrophages and endothelial cells were established in this study as the major cellular sources of hBD-3 in septic loosening, but fibroblasts and foreign body giant cells can also contribute to its production. The heterogeneous topological staining of hBD-3 suggests local regulation, possibly by bacterial products, damage-associated molecular patterns and cytokines. The results explain the increased synovial fluid/tissue concentrations of hBD-3 in septic loosening.
authors: Department of Anatomy, Institute of Biomedicine, BMH 1, PO Box 63, 00014, Helsinki, Finland, jhlevo@utu.fi.
authors: Jaakko
authors: J
authors: Levón
authors: Ahmed
authors: A
authors: Al-Samadi
authors: Zygmunt
authors: Z
authors: Mackiewicz
authors: Andrej
authors: A
authors: Coer
authors: Rihard
authors: R
authors: Trebse
authors: Eero
authors: E
authors: Waris
authors: Yrjö T
authors: YT
authors: Konttinen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006622:Hip Prosthesis / Q000009:adverse effects; D006801:Humans; D007150:Immunohistochemistry; D007720:Knee Prosthesis / Q000009:adverse effects; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D011475:Prosthesis Failure / Q000209:etiology*; D016459:Prosthesis-Related Infections / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D011817:Rabbits; D018805:Sepsis / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D013582:Synovial Fluid / Q000378:metabolism; D023083:beta-Defensins / Q000096:biosynthesis*
chemical_list: C422950:DEFB103A protein, human; D023083:beta-Defensins
references: J Histochem Cytochem. 1985 Nov;33(11):1110-5
references: 3902962
references: Cell Tissue Res. 2001 Nov;306(2):257-64
references: 11702237
references: Science. 1987 Sep 25;237(4822):1588-95
references: 3629258
references: Eur J Immunol. 2007 Sep;37(9):2474-86
references: 17705135
references: J Dermatol Sci. 2004 Nov;36(2):87-95
references: 15519138
references: J Arthroplasty. 2011 Dec;26(8):1136-8
references: 22075161
references: J Rheumatol. 2003 Aug;30(8):1719-24
references: 12913926
references: Biomaterials. 2012 Sep;33(26):5967-82
references: 22695065
references: Orthopedics. 2012 Jan 16;35(1):e53-60
references: 22229614
references: Antimicrob Agents Chemother. 2006 Feb;50(2):806-9
references: 16436752
references: J Biol Chem. 2001 Feb 23;276(8):5707-13
references: 11085990
references: Pharmaceuticals (Basel). 2014 May 13;7(5):545-94
references: 24828484
references: BMC Microbiol. 2008 Nov 26;8:205
references: 19036162
references: Orthopedics. 2014 Apr;37(4):e384-90
references: 24762845
references: Best Pract Res Clin Rheumatol. 2006 Dec;20(6):1045-63
references: 17127196
references: Eur J Oral Sci. 2002 Apr;110(2):121-4
references: 12013554
references: J Innate Immun. 2012;4(4):337-48
references: 22441423
references: J Pathol. 2002 Nov;198(3):369-77
references: 12375270
references: J Arthroplasty. 2008 Oct;23(7):984-91
references: 18534466
references: J Immunol. 2010 Jun 15;184(12):6688-94
references: 20483750
references: Arch Oral Biol. 2013 Oct;58(10 ):1415-21
references: 23726315
references: J Periodontal Res. 2005 Dec;40(6):474-81
references: 16302926
references: J Clin Pathol. 1998 Jul;51(7):506-11
references: 9797726
references: J Dent Res. 2005 May;84(5):445-50
references: 15840781
references: J Immunol. 2013 Jul 15;191(2):865-74
references: 23776172
references: Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18631-5
references: 18006661
references: Mol Med Rep. 2014 Aug;10(2):825-31
references: 24913184
references: Biochem J. 2006 Oct 1;399(1):1-7
references: 16956326
references: Biochim Biophys Acta. 2006 Sep;1758(9):1499-512
references: 16978580
references: J Surg Res. 2014 Jan;186(1):99-100
references: 23639356
references: J Biomed Mater Res A. 2007 Jun 15;81(4):1017-26
references: 17415764
references: J Bone Joint Surg Am. 2013 Apr 3;95(7):644-51
references: 23553300
references: Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8880-5
references: 12840147
references: Future Microbiol. 2011 Nov;6(11):1329-49
references: 22082292
references: Arch Pathol Lab Med. 1979 Mar;103(3):135-8
references: 85440
references: J Rheumatol. 2009 Mar;36(3):598-608
references: 19208601
references: Acta Orthop. 2012 Jun;83(3):299-304
references: 22616742
references: Front Immunol. 2012 Jul 17;3:195
references: 22822405
references: Acta Orthop. 2013 Dec;84(6):524-9
references: 24237422
references: N Engl J Med. 2009 Aug 20;361(8):787-94
references: 19692690
references: J Endod. 2013 Dec;39(12):1625-9
references: 24238461