title: Non-contact acoustic radiation force impulse microscopy via photoacoustic detection for probing breast cancer cell mechanics.
abstract: We demonstrate a novel non-contact method: acoustic radiation force impulse microscopy via photoacoustic detection (PA-ARFI), capable of probing cell mechanics. A 30 MHz lithium niobate ultrasound transducer is utilized for both detection of phatoacoustic signals and generation of acoustic radiation force. To track cell membrane displacements by acoustic radiation force, functionalized single-walled carbon nanotubes are attached to cell membrane. Using the developed microscopy evaluated with agar phantoms, the mechanics of highly- and weakly-metastatic breast cancer cells are quantified. These results clearly show that the PA-ARFI microscopy may serve as a novel tool to probe mechanics of single breast cancer cells.
authors: Department of Information and Communication Engineering, Daegu Gyeongbuk Institute of Science & Technology, Daegu, South Korea.
authors: Jae Youn
authors: JY
authors: Hwang
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
authors: Bong Jin
authors: BJ
authors: Kang
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
authors: Changyang
authors: C
authors: Lee
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
authors: Hyung Ham
authors: HH
authors: Kim
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA ; qifazhou@usc.edu.
authors: Jinhyoung
authors: J
authors: Park
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA ; Co-corresponding authors ; qifazhou@usc.edu.
authors: Qifa
authors: Q
authors: Zhou
authors: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
authors: K Kirk
authors: KK
authors: Shung
keywords: (170.0180) Microscopy; (170.5120) Photoacoustic imaging; (170.7170) Ultrasound
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