title: Pretreatment with scutellaria baicalensis stem-leaf total flavonoid protects against cerebral ischemia/reperfusion injury in hippocampal neurons.
abstract: Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid (SSTF). However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric administration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderate- and high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Xiangyu
authors: X
authors: Kong
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Wei
authors: W
authors: Kong
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Guangxin
authors: G
authors: Miao
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Shumin
authors: S
authors: Zhao
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Meng
authors: M
authors: Chen
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Xiaoying
authors: X
authors: Zheng
authors: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China.
authors: Jiangtao
authors: J
authors: Bai
keywords: apoptosis; cerebral ischemia/reperfusion; free radicals; hippocampus; nerve regeneration; neural regeneration; pretreatment; scutellaria baicalensis stem-leaf total flavonoid; vascular permeability
references: J Clin Invest. 2000 Sep;106(6):723-31
references: 10995780
references: Stroke. 1989 Jan;20(1):84-91
references: 2643202
references: Pediatr Res. 2006 Aug;60(2):169-73
references: 16864698
references: Neurochem Int. 2008 Sep;53(3-4):95-101
references: 18598728
references: Neurosci Biobehav Rev. 1997 Mar;21(2):207-17
references: 9062944
references: J Neurosci. 1999 May 15;19(10):3809-17
references: 10234013
references: Neural Regen Res. 2013 Dec 5;8(34):3183-92
references: 25206639
references: J Anat. 2005 Apr;206(4):405-10
references: 15817108
references: J Hypertens. 2008 Jul;26(7):1435-45
references: 18551021