title: Characterization of mouse model-derived osteosarcoma (OS) cells in vitro and in vivo.
abstract: Osteosarcoma (OS) is the most common primary tumor of bone with a high incidence in children. Treatment options for OS are limited, and once metastasized, the prognosis is very poor. Genetically engineered mouse models (GEMMs) are valuable tools to understand the mechanisms of tumorigenesis and to test possible therapies. In this chapter, we summarize the methods related to the isolation, characterization, and transplantation of OS cells obtained from GEMMs.
authors: BBVA Foundation-CNIO Cancer Cell Biology Program, Spanish National Cancer Research Centre (CNIO), Madrid, 28029, Spain.
authors: Özge
authors: Ö
authors: Uluçkan
authors: Latifa
authors: L
authors: Bakiri
authors: Erwin F
authors: EF
authors: Wagner
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001859:Bone Neoplasms / Q000473:pathology; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D051379:Mice; D012516:Osteosarcoma / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D000880:Anthraquinones; C010078:alizarin; D000469:Alkaline Phosphatase