title: HIV microarray for the mapping and characterization of HIV-specific antibody responses.
abstract: We used the microarray technology to develop chips containing a comprehensive set of proteins and peptides covering the proteome of HIV-1 clade C, which is the HIV-1 subtype that causes the majority of infections worldwide. We demonstrate that the HIV microarray allows simultaneous, sensitive and specific detection of antibody responses for the major immunoglobulin classes (IgG, IgA, IgM, IgE) and subclasses (IgG1-4) with minute amounts of serum samples towards a large number of HIV antigens and peptides. Furthermore, we show that the HIV chip can be used for the monitoring of antibody responses during the course of the disease and during treatment. The HIV microarray should be useful to study antibody responses to multiple HIV antigens and epitopes in HIV-infected patients to explore pathomechanisms of the disease, for diagnosis and for monitoring of treatment and of vaccine trials.
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Währinger Gürtel 18-20, 3Q, 1090 Vienna, Austria. rudolf.valenta@meduniwien.ac.at.
authors: Daniela
authors: D
authors: Gallerano
authors: Eva
authors: E
authors: Wollmann
authors: Christian
authors: C
authors: Lupinek
authors: Thomas
authors: T
authors: Schlederer
authors: Daniel
authors: D
authors: Ebner
authors: Christian
authors: C
authors: Harwanegg
authors: Katarzyna
authors: K
authors: Niespodziana
authors: Klaus
authors: K
authors: Schmetterer
authors: Winfried
authors: W
authors: Pickl
authors: Elisabeth
authors: E
authors: Puchhammer-Stöckl
authors: Elopy
authors: E
authors: Sibanda
authors: Rudolf
authors: R
authors: Valenta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D015658:HIV Infections / Q000097:blood / Q000628:therapy; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics; D013997:Time Factors; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014764:Viral Proteins