title: Generation of a persistently infected MDBK cell line with natural bovine spongiform encephalopathy (BSE).
abstract: Bovine spongiform encephalopathy (BSE) is a zoonotic transmissible spongiform encephalopathy (TSE) thought to be caused by the same prion strain as variant Creutzfeldt-Jakob disease (vCJD). Unlike scrapie and chronic wasting disease there is no cell culture model allowing the replication of proteinase K resistant BSE (PrPBSE) and the further in vitro study of this disease. We have generated a cell line based on the Madin-Darby Bovine Kidney (MDBK) cell line over-expressing the bovine prion protein. After exposure to naturally BSE-infected bovine brain homogenate this cell line has shown to replicate and accumulate PrPBSE and maintain infection up to passage 83 after initial challenge. Collectively, we demonstrate, for the first time, that the BSE agent can infect cell lines over-expressing the bovine prion protein similar to other prion diseases. These BSE infected cells will provide a useful tool to facilitate the study of potential therapeutic agents and the diagnosis of BSE.
authors: Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Dongseob
authors: D
authors: Tark
authors: Department of Animal Disease Control and Quarantine, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Hyojin
authors: H
authors: Kim
authors: Pathology and Host Susceptibility Department, Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom.
authors: Michael H
authors: MH
authors: Neale
authors: Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Minjeong
authors: M
authors: Kim
authors: Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Hyunjoo
authors: H
authors: Sohn
authors: Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Yoonhee
authors: Y
authors: Lee
authors: Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Insoo
authors: I
authors: Cho
authors: Department of Animal Disease Control and Quarantine, Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors: Yiseok
authors: Y
authors: Joo
authors: Pathology and Host Susceptibility Department, Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom.
authors: Otto
authors: O
authors: Windl
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics; D016643:Encephalopathy, Bovine Spongiform / Q000235:genetics*; D005821:Genetic Techniques; D016086:Lentivirus / Q000235:genetics; D011328:Prions / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D004274:DNA, Recombinant; D011328:Prions
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