title: Loss of wwox expression in zebrafish embryos causes edema and alters Ca(2+) dynamics.
abstract: We investigated the role of the WW domain-containing oxidoreductase (wwox) gene in the embryonic development of zebrafish, with particular emphasis on intracellular Ca(2+) dynamics because Ca(2+) is an important intracellular messenger. Comparisons between zebrafish wwox and human WWOX sequences identified highly conserved domain structures. wwox was expressed in developing heart tissues in the zebrafish embryo. Moreover, wwox knockdown induced pericardial edema with similarities to conditions observed in human breast cancer. The wwox knockdown embryos with the edema died within a week. High Ca(2+) levels were observed at the boundary between the edema and yolk in wwox knockdown embryos.
authors: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) , Yokosuka , Japan.
authors: Yusuke
authors: Y
authors: Tsuruwaka
authors: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST) , Nomi, Ishikawa , Japan.
authors: Masataka
authors: M
authors: Konishi
authors: Department of Animal Science, University of California , Davis, CA , USA.
authors: Eriko
authors: E
authors: Shimada
keywords: Intracellular calcium; WWOX; Yellow cameleon; Zebrafish
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