title: A high boronate avidity monolithic capillary for the selective enrichment of trace glycoproteins.
abstract: Boronate affinity materials, as effective sample enrichment sorbents for glycoproteomic analysis, have attracted increasing attention in recent years. However, most of boronate affinity materials suffer from an apparent limitation, limited binding strength. As a result, extraction of glycoproteins of trace concentration is rather difficult or impossible. In this study, we present a high boronate avidity monolithic capillary. Branched polyethyleneimine (PEI) was used as a scaffold to amplify the number of boronic acid moieties. While 2,4-difluoro-3-formyl-phenylboronic acid (DFFPBA), which exhibited ultrahigh affinity toward cis-diol-containing compounds, was employed as an affinity ligand. Due to the PEI-assisted synergistic multivalent binding, the monolithic column exhibited high boronate avidity toward glycoproteins, with binding constants of 10(-6)-10(-7)M. Such binding strength was the highest among already reported boronic acid-functionalized materials that can be used for glycoproteomic analysis. Besides, the boronate avidity monolithic column exhibited one additional beneficial feature, lowered binding pH (≥6.5). These features greatly favored the selective enrichment of trace glycoproteins from real samples. The feasibility for practical applications was demonstrated with the selective enrichment of trace glycoproteins in human saliva. As compared with other boronate avidity/affinity materials, the boronate avidity monolithic capillary exhibited the best performance.
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China.
authors: Daojin
authors: D
authors: Li
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors: Yang
authors: Y
authors: Li
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China.
authors: Xinglin
authors: X
authors: Li
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China.
authors: Zijun
authors: Z
authors: Bie
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China.
authors: Xianghua
authors: X
authors: Pan
authors: Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China.
authors: Qian
authors: Q
authors: Zhang
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China; Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China. Electronic address: zhenliu@nju.edu.cn.
authors: Zhen
authors: Z
authors: Liu
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D012463:Saliva / Q000737:chemistry
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins; C010686:benzeneboronic acid
keywords: Binding strength; Boronic acid; Enrichment; Glycoprotein; Monolithic column; Synergistic multiple binding