title: Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase.
abstract: Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.
authors: a Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering , Hokkaido University , Sapporo , Japan.
authors: Toshinori
authors: T
authors: Yokoo
authors: Ken'ichiro
authors: K
authors: Matsumoto
authors: Takashi
authors: T
authors: Ooba
authors: Kenjiro
authors: K
authors: Morimoto
authors: Seiichi
authors: S
authors: Taguchi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D055162:Biocatalysis; D002460:Cell Line; D006885:Hydroxybutyrates / Q000378:metabolism*; D007700:Kinetics; D030821:Plants, Genetically Modified; D011091:Polyesters / Q000378:metabolism*; D015202:Protein Engineering; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics*; D014170:Transformation, Genetic; D000094122:Polyhydroxybutyrates
chemical_list: D000429:Alcohol Oxidoreductases; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023744:acetoacetyl-CoA reductase
keywords: biodegradable plastic; polyhydroxyalkanoate; polyhydroxybutyrate