title: Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus.
abstract: The in vivo functions of Hje and Hjc, two Holliday junction resolvases in Sulfolobus islandicus were investigated. We found that deletion of either hje or hjc had no effect on normal cell growth, while deletion of both hje and hjc is lethal. Although Hjc is the conserved resolvase in all archaea, the hje deletion rather than hjc deletion rendered cells more sensitive to DNA-damaging agents such as hydroxyurea, cisplatin, and methyl methanesulfonate than the wild type (WT). Intriguingly, the sensitivity of Δhje could not be rescued by ectopic expression of Hje from a plasmid and Hje overexpression slowed growth and large cells appeared with more than two genome equivalents. We showed that Hje was maintained at a low level in WT cells. Furthermore, transcriptomic microarray analysis revealed that the abundance of transcripts of many genes including those involved in DNA replication, repair, transcription regulation, and cell division changed drastically in the Hje-overexpressed strain. However, only limited genes were up- or downregulated in the hje deletion strain. Our findings collectively suggest that Hje is the primary resolvase involved in DNA repair and its expression must be tightly controlled in cells.
authors: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., Jinan, 250100, People's Republic of China.
authors: Qihong
authors: Q
authors: Huang
authors: Yansheng
authors: Y
authors: Li
authors: Chaoning
authors: C
authors: Zeng
authors: Tengteng
authors: T
authors: Song
authors: Zhou
authors: Z
authors: Yan
authors: Jinfeng
authors: J
authors: Ni
authors: Qunxin
authors: Q
authors: She
authors: Yulong
authors: Y
authors: Shen
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D045565:Holliday Junction Resolvases / Q000235:genetics* / Q000378:metabolism; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D045565:Holliday Junction Resolvases
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