title: Mangiferin ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in mouse model of postoperative ileus.
abstract: Our previous study has shown that mangiferin (MGF), a glucosylxanthone from Mangifera indica, exerts gastrointestinal prokinetic action involving a cholinergic mechanism. Postoperative ileus (POI) is a temporary disturbance in gastrointestinal motility following surgery, and intestinal inflammatory response plays a critical role in the pathogenesis of POI. The present study investigated to know whether MGF having anti-inflammatory and prokinetic actions can ameliorate the intestinal inflammation and impaired gastrointestinal transit seen in the mouse model of POI. Experimental POI was induced in adult male Swiss mice by standardized small intestinal manipulation (IM). Twenty-four hours later, gastrointestinal transit was assessed by charcoal transport. MGF was administered orally 1 h before the measurement of GIT. To evaluate the inflammatory response, plasma levels of proinflammatory cytokines TNF-α, IL-1β, IL-6, and chemokine MCP-1, and the myeloperoxidase activity, nitrate/nitrite level, and histological changes of ileum were determined in mice treated or not with MGF. Experimental POI in mice was characterized by decreased gastrointestinal transit and marked intestinal and systemic inflammatory response. MGF treatment led to recovery of the delayed intestinal transit induced by IM. MGF in ileum significantly inhibited the myeloperoxidase activity, a marker of neutrophil infiltration, and nitrate/nitrite level and reduced the plasma levels of TNF-α, IL-1β, IL-6, and MCP-1 as well. MGF treatment ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in the mouse model of POI.
authors: Department of Physiology and Pharmacology, Faculty of Medicine, INCT-IBISAB-Brazilian Semi-Arid Institute of Biomedicine, 60430-270, Fortaleza, Ceará, Brazil.
authors: Talita Cavalcante
authors: TC
authors: Morais
authors: Bruno Rodrigues
authors: BR
authors: Arruda
authors: Hebert
authors: H
authors: de Sousa Magalhães
authors: Maria Teresa Salles
authors: MT
authors: Trevisan
authors: Daniel
authors: D
authors: de Araújo Viana
authors: Vietla Satyanarayana
authors: VS
authors: Rao
authors: Flavia Almeida
authors: FA
authors: Santos
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004760:Enterocolitis / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005765:Gastrointestinal Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007082:Ileum / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D045823:Ileus / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D031022:Mangifera / Q000737:chemistry; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011183:Postoperative Complications / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D044004:Xanthones / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*
chemical_list: D016207:Cytokines; D005765:Gastrointestinal Agents; D044004:Xanthones; C013592:mangiferin
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