title: Noncovalent hydrogel beads as microcarriers for cell culture.
abstract: Hydrogel beads as microcarriers could have many applications in biotechnology. However, bead formation by noncovalent cross-linking to achieve high cell compatibility by avoiding chemical reactions remains challenging because of rapid gelation rates and/or low stability. Here we report the preparation of homogeneous, tunable, and robust hydrogel beads from peptide-polyethylene glycol conjugates and oligosaccharides under mild, cell-compatible conditions using a noncovalent crosslinking mechanism. Large proteins can be released from beads easily. Further noncovalent modification allows for bead labeling and functionalization with various compounds. High survival rates of embedded cells were achieved under standard cell culture conditions and after freezing the beads, demonstrating its suitability for encapsulating and conserving cells. Hydrogel beads as functional system have been realized by generating protein-producing microcarriers with embedded eGFP-secreting insect cells.
authors: B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307 Dresden (Germany).
authors: Robert
authors: R
authors: Wieduwild
authors: Swati
authors: S
authors: Krishnan
authors: Karolina
authors: K
authors: Chwalek
authors: Annett
authors: A
authors: Boden
authors: Mirko
authors: M
authors: Nowak
authors: David
authors: D
authors: Drechsel
authors: Carsten
authors: C
authors: Werner
authors: Yixin
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D001709:Biotechnology; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D004337:Drug Carriers / Q000138:chemical synthesis*; D004339:Drug Compounding; D005615:Freezing; D049452:Green Fluorescent Proteins; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D007313:Insecta; D046210:Microfluidic Analytical Techniques; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D020100:Hydrogels; D009844:Oligosaccharides; D010455:Peptides; D013501:Surface-Active Agents; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols
keywords: beads; cell encapsulation; gels; noncovalent interactions; protein release