title: Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products.
abstract: Genetic manipulation of bacteria often requires the joining together of more than one DNA segment to form a hybrid DNA molecule. This can be accomplished by PCR followed by restriction endonuclease digestions and ligations. However, this approach can often become laborious and expensive. Here is described a well-established method for using primer design and PCR to obtain hybrid products for use in cloning vectors, mutagenesis protocols, and other applications.
authors: Department of Biological Sciences, Mississippi State University, 295 E. Lee Blvd., Mississippi State, MS, 39762, USA. thornton@biology.msstate.edu.
authors: Justin A
authors: JA
authors: Thornton
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012326:RNA Splicing / Q000235:genetics*; D013210:Staphylococcus / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D017931:DNA Primers
keywords: Gene splicing; Mutagenesis; PCR fusion; Polymerase chain reaction