title: Comparison of the effects of fresh leaf and peel extracts of walnut (Juglans regia L.) on blood glucose and β-cells of streptozotocin-induced diabetic rats.
abstract: There is some report about the hypoglycemic effect of Juglans rejia L. leaf in alloxan induced diabetic rats and hypoglycemic effect of its fruit peel administered intra peritoneally. Thirty male Wistar rats divided into five groups, to evaluate the hypoglycemic and pancreas β-cells regenerative effects of oral methanolic extracts of leaf and fruit peel of walnut. Rats were made diabetic by intravenous (IV) injection of 50 mg kg(-1) streptozotocin (STZ). Negative control group did not get STZ and any treatment. Positive control, leaf extract, peel extract and insulin groups were treated orally by extract solvent, 200 mg kg(-1) leaf extract, 200 mg kg(-1) peel extract and 5 IU kg(-1) of subcutaneous neutral protamine Hagedorn (NPH) insulin, respectively. Four weeks later, blood was collected for biochemical analysis and pancreases were removed for β-cells counts in histological sections. Diabetes leads to increase of fast blood sugar (FBS) and HbA1c, and decrease of β-cell number and insulin. FBS decreased only in leaf extract group. HbA1c decreased in leaf extract and insulin groups. The β-cells number increased in leaf and peel extract groups. Insulin increased moderately in all treatment groups. We showed the proliferative properties of leaves and peel of Juglans regia L. methanolic extract in STZ- induced diabetic rats, which was accompanied by hypoglycemic effect of leaf extract.
authors: Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
authors: Somaye
authors: S
authors: Javidanpour
authors: Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
authors: Seyed Reza
authors: SR
authors: Fatemi Tabtabaei
authors: Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors: Amir
authors: A
authors: Siahpoosh
authors: Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
authors: Hasan
authors: H
authors: Morovati
authors: Department of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
authors: Ali
authors: A
authors: Shahriari
keywords: Diabetes; Hypoglycemia; Insulin; Walnut leaf; Walnut peel
references: Biol Trace Elem Res. 1990 Mar;24(3):183-8
references: 1702671
references: Plant Foods Hum Nutr. 2001;56(1):75-82
references: 11213171
references: Food Chem Toxicol. 2008 Jun;46(6):2103-11
references: 18334279
references: Mycoses. 1999;42(11-12):665-72
references: 10680445
references: J Med Food. 2008 Sep;11(3):533-8
references: 18800903
references: Food Chem Toxicol. 2007 Nov;45(11):2287-95
references: 17637491
references: Eur J Clin Microbiol Infect Dis. 2010 Jan;29(1):81-8
references: 19899011
references: J Nutr. 1980 May;110(5):931-6
references: 6989965
references: Metabolism. 1973 Feb;22(2):269-71
references: 4687947
references: N Engl J Med. 1976 Aug 19;295(8):443-4
references: 934243
references: Diabetes Care. 2001 Nov;24(11):1936-40
references: 11679460
references: Am J Vet Res. 1991 Dec;52(12):2060-6
references: 1665025
references: Br J Nutr. 1999 Mar;81(3):203-9
references: 10434846
references: Drugs. 1997 Sep;54(3):355-68
references: 9279500
references: J Biol Chem. 1987 May 5;262(13):6238-47
references: 3553187
references: Am J Manag Care. 2003 Mar;9(3 Suppl):S63-80; quiz S81-4
references: 12653455
references: Afr J Tradit Complement Altern Med. 2007 Feb 16;4(3):299-305
references: 20161893
references: J Ethnopharmacol. 2003 Nov;89(1):123-9
references: 14522443
references: J Ethnopharmacol. 1990 Oct;30(3):265-79
references: 2259215