title: Canine distemper virus DNA vaccination of mink can overcome interference by maternal antibodies.
abstract: Canine distemper virus (CDV) is highly contagious and can cause severe disease against which conventional live vaccines are ineffective in the presence of maternal antibodies. Vaccination in the presences of maternal antibodies was challenged by vaccination of 5 days old and 3 weeks old mink kits with CDV DNA vaccines. Virus neutralising (VN) antibody responses were induced in mink kits vaccinated with a plasmid encoding the haemaglutinin protein (H) of CDV (n=5, pCDV-H) or a combination of the H, fusion (F) and nucleoprotein (N) of CDV (n=5, pCDV-HFN). These DNA vaccinated kits were protected against virulent experimental infection with field strains of CDV. The pCDV-H was more efficient in inducing protective immunity in the presence of maternal antibodies compared to the pCDV-HFN. The results show that DNA vaccination with the pCDV-H or pCDV-HFN (n=4) only given once at 5 days of age induces virus specific immune response in neonatal mink and protection against virulent CDV exposure later in life.
authors: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, DK-1870 Frederiksberg C, Denmark; Department of Chemistry and Bioscience, Faculty of Engineering and Sciences, Aalborg University/Aalborg Zoo, Frederik Bajers Vej 7H, DK-9220 Aalborg, Denmark(1). Electronic address: trine@bio.aau.dk.
authors: Trine Hammer
authors: TH
authors: Jensen
authors: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, DK-1870 Frederiksberg C, Denmark.
authors: Line
authors: L
authors: Nielsen
authors: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, DK-1870 Frederiksberg C, Denmark.
authors: Bent
authors: B
authors: Aasted
authors: Department of Chemistry and Bioscience, Faculty of Engineering and Sciences, Aalborg University/Aalborg Zoo, Frederik Bajers Vej 7H, DK-9220 Aalborg, Denmark(1).
authors: Cino
authors: C
authors: Pertoldi
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 København N, Denmark.
authors: Merete
authors: M
authors: Blixenkrone-Møller
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000276:immunology; D004216:Distemper / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology* / Q000472:pathogenicity; D006389:Hemagglutinins, Viral; D007112:Immunity, Maternally-Acquired; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008907:Mink / Q000821:virology*; D009500:Neutralization Tests; D009698:Nucleoproteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D009698:Nucleoproteins; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Canine distemper virus; DNA vaccination; Maternal antibodies; Mink; Morbillivirus; Neonatal