title: Ocular biocompatibility of polyquaternium 10 gel: functional and morphological results.
abstract: This paper deals with the characterization study of topical and intraocular biocompatibility and toxicity of cationic hydroxyethylcellulose Polyquaternium 10 (PQ10). It also evaluates the rheological properties of gels. The cytotoxicity assays were done in two cell lines: HEp-2 and VERO (human larynx epidermoid carcinoma cell and African green monkey kidney cells respectively). For the in vivo study, New Zealand albino rabbits were used. The in vitro cytotoxic activity of PQ10 shows no statistically significant differences in relation to the control of hydroxypropylmethylcellulose (HPMC) in any of the cell lines used in this study. Similarly, the signs of inflammation observed after treatment showed no significant difference between the groups of animals treated with the polymer compared to the control group. Normal histological characteristics were seen in both groups with no histological inflammatory reaction. After 1 month of the intracameral application of 2% PQ10 (treatment group) or 0.3% HPMC (control group), electroretinograms showed similar levels of a- and b-waves latencies and amplitude. In summary, PQ10 gel was well tolerated in these experiments, with proper monitoring, it could stand as a new alternative in the development of ophthalmic viscosurgical devices.
authors: Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR), Ministerio de Ciencia y Tecnología de Córdoba, Pabellón CEPROCOR, CP 5164, Santa María de Punilla, Córdoba, Argentina.
authors: Roxana Valeria
authors: RV
authors: Alasino
authors: Luciana Guadalupe
authors: LG
authors: Garcia
authors: Ana Laura
authors: AL
authors: Gramajo
authors: Juan Pablo
authors: JP
authors: Pusterla
authors: Dante Miguel
authors: DM
authors: Beltramo
authors: José Domingo
authors: JD
authors: Luna
mesh_terms: D060433:Administration, Ophthalmic; D000287:Administration, Topical; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D002522:Chlorocebus aethiops; D055119:Elastic Modulus; D005123:Eye / Q000187:drug effects* / Q000473:pathology*; D005782:Gels / Q000737:chemistry / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D009883:Ophthalmic Solutions / Q000737:chemistry* / Q000633:toxicity*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000633:toxicity*; D011817:Rabbits; D014709:Vero Cells; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D009883:Ophthalmic Solutions; C506623:PQ10 compound; D000644:Quaternary Ammonium Compounds; D002482:Cellulose
references: Arch Ophthalmol. 2006 Oct;124(10):1441-8
references: 17030712
references: J Biomed Mater Res A. 2006 Aug;78(2):394-8
references: 16680700
references: Nature. 2004 Apr 1;428(6982):487-92
references: 15057821
references: Acta Ophthalmol (Copenh). 1979;57(6):1004-13
references: 545996
references: Indian J Pharm Sci. 2009 May;71(3):242-51
references: 20490289
references: J Biomed Mater Res A. 2006 Dec 15;79(4):963-73
references: 16948146
references: Acta Ophthalmol (Copenh). 1991 Feb;69(1):50-6
references: 1903016
references: J Cataract Refract Surg. 1999 Feb;25(2):167-73
references: 9951659
references: Chem Rev. 2001 Jul;101(7):1869-79
references: 11710233
references: Transplantation. 2005 Dec 15;80(11):1586-95
references: 16371930
references: Biomacromolecules. 2006 Jun;7(6):1935-41
references: 16768417
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Ophthalmology. 2004 Mar;111(3):476-82
references: 15019322
references: J Biomed Mater Res A. 2010 Mar 15;92(4):1421-30
references: 19358259
references: Biomacromolecules. 2006 Jun;7(6):1819-28
references: 16768403
references: Biomaterials. 2002 Jan;23(2):457-62
references: 11761166
references: Nanomedicine (Lond). 2010 Apr;5(3):469-84
references: 20394538
references: J Biomed Mater Res A. 2005 Oct 1;75(1):156-67
references: 16052490
references: Eur J Pharm Biopharm. 2003 Jul;56(1):133-42
references: 12837491
references: Adv Mater. 2009 Sep 4;21(32-33):3307-29
references: 20882499