title: Inhibition of Anopheles gambiae odorant receptor function by mosquito repellents.
abstract: The identification of molecular targets of insect repellents has been a challenging task, with their effects on odorant receptors (ORs) remaining a debatable issue. Here, we describe a study on the effects of selected mosquito repellents, including the widely used repellent N,N-diethyl-meta-toluamide (DEET), on the function of specific ORs of the African malaria vector Anopheles gambiae. This study, which has been based on quantitative measurements of a Ca(2+)-activated photoprotein biosensor of recombinant OR function in an insect cell-based expression platform and a sequential compound addition protocol, revealed that heteromeric OR (ORx/Orco) function was susceptible to strong inhibition by all tested mosquito repellents except DEET. Moreover, our results demonstrated that the observed inhibition was due to efficient blocking of Orco (olfactory receptor coreceptor) function. This mechanism of repellent action, which is reported for the first time, is distinct from the mode of action of other characterized insect repellents including DEET.
authors: From the Insect Molecular Genetics and Biotechnology Group, Institute of Biosciences and Applications, National Centre for Scientific Research "Demokritos", 15310 Athens and.
authors: Panagiota
authors: P
authors: Tsitoura
authors: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 70013 Heraklion, Crete, Greece.
authors: Konstantinos
authors: K
authors: Koussis
authors: From the Insect Molecular Genetics and Biotechnology Group, Institute of Biosciences and Applications, National Centre for Scientific Research "Demokritos", 15310 Athens and iatrou@bio.demokritos.gr.
authors: Kostas
authors: K
authors: Iatrou
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism / Q000502:physiology*; D007302:Insect Repellents; D018035:Receptors, Odorant / Q000378:metabolism*
chemical_list: D007302:Insect Repellents; D018035:Receptors, Odorant
keywords: Biosensor; Chemical Biology; High-throughput Screening (HTS); Insect; Lepidopteran Insect Cells; Mosquito Olfaction; Olfactory Receptors; Orco Co-receptor Antagonism; Recombinant Protein Expression
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