title: An UPLC-MS/MS method for determination of solasonine in rat plasma and its application of a pharmacokinetic and bioavailability study.
abstract: Solasonine, a known glycoalkaloid, is a potential anti-cancer agent. In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of solasonine in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an ACQUITY HSS T3 (100×2.1mm, 1.8μm) column with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1-500ng/mL (R(2)>0.994) with a low limit of detection (0.1ng/mL) and lower limit of quantification (0.5ng/mL). The extraction recovery was in the range of 92.4-94.9% for solasonine and 91.9% for dendrobine (internal standard, IS). The intra- and inter-day precision was below 9.8% and accuracy was from 86.0% to 94.3%. No notable matrix effect and astaticism was observed for solasonine. The method has been successfully applied to a pharmacokinetic and bioavailability study of solasonine in rats for the first time, which provides the basis for the further development and application of solasonine.
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China.
authors: Yuanyuan
authors: Y
authors: Chen
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China.
authors: Shuangshuang
authors: S
authors: Zhang
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China.
authors: Dahui
authors: D
authors: Chen
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China.
authors: Mi
authors: M
authors: Zhou
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China.
authors: Jing
authors: J
authors: Zheng
authors: School of Pharmaceutical Sciences, Wenzhou Medical University/, Wenzhou 325035, China. Electronic address: XZH0077@126.com.
authors: Zheng
authors: Z
authors: Xiang
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012991:Solanaceous Alkaloids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012991:Solanaceous Alkaloids; C026608:alpha-solamargine
keywords: Bioavailability; Pharmacokinetics; Solasonine; UPLC-MS/MS