title: Isoform-specific Na,K-ATPase alterations precede disuse-induced atrophy of rat soleus muscle.
abstract: This study examines the isoform-specific effects of short-term hindlimb suspension (HS) on the Na,K-ATPase in rat soleus muscle. Rats were exposed to 24-72 h of HS and we analyzed the consequences on soleus muscle mass and contractile parameters; excitability and the resting membrane potential (RMP) of muscle fibers; the electrogenic activity, protein, and mRNA content of the α1 and α2 Na,K-ATPase; the functional activity and plasma membrane localization of the α2 Na,K-ATPase. Our results indicate that 24-72 h of HS specifically decreases the electrogenic activity of the Na,K-ATPase α2 isozyme and the RMP of soleus muscle fibers. This decrease occurs prior to muscle atrophy or any change in contractile parameters. The α2 mRNA and protein content increased after 24 h of HS and returned to initial levels at 72 h; however, even the increased content was not able to restore α2 enzyme activity in the disused soleus muscle. There was no change in the membrane localization of α2 Na,K-ATPase. The α1 Na,K-ATPase electrogenic activity, protein and mRNA content did not change. Our findings suggest that skeletal muscle use is absolutely required for α2 Na,K-ATPase transport activity and provide the first evidence that Na,K-ATPase alterations precede HS-induced muscle atrophy.
authors: St. Petersburg State University, 7/9 University emb., St. Petersburg 199034, Russia.
authors: Violetta V
authors: VV
authors: Kravtsova
authors: Aarhus University, Ole Worms Alle bygn. 4, 1163, C 8000 Aarhus, Denmark.
authors: Vladimir V
authors: VV
authors: Matchkov
authors: Aarhus University, Ole Worms Alle bygn. 4, 1163, C 8000 Aarhus, Denmark.
authors: Elena V
authors: 0000-0001-9511-8009
authors: EV
authors: Bouzinova
authors: St. Petersburg State University, 7/9 University emb., St. Petersburg 199034, Russia.
authors: Alexander N
authors: AN
authors: Vasiliev
authors: St. Petersburg State University, 7/9 University emb., St. Petersburg 199034, Russia.
authors: Irina A
authors: IA
authors: Razgovorova
authors: University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
authors: Judith A
authors: JA
authors: Heiny
authors: St. Petersburg State University, 7/9 University emb., St. Petersburg 199034, Russia.
authors: Igor I
authors: II
authors: Krivoi
mesh_terms: D000818:Animals; D001835:Body Weight; D019417:Hindlimb Suspension; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology* / Q000503:physiopathology; D020966:Muscular Disorders, Atrophic / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D009538:Nicotine / Q000494:pharmacology; D009929:Organ Size; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D009538:Nicotine; D000254:Sodium-Potassium-Exchanging ATPase
references: Muscle Nerve. 2011 Dec;44(6):854-61
references: 22102453
references: Am J Physiol Cell Physiol. 2001 Mar;280(3):C509-16
references: 11171570
references: Prog Neurobiol. 2001 Jul;64(4):393-429
references: 11275359
references: Am J Physiol Regul Integr Comp Physiol. 2009 Jan;296(1):R125-32
references: 18987285
references: J Cell Physiol. 2007 Aug;212(2):509-18
references: 17458903
references: Int J Biochem Cell Biol. 2013 Oct;45(10):2200-8
references: 23800384
references: J Exp Biol. 2005 Dec;208(Pt 24):4585-92
references: 16326940
references: J Biol Chem. 2012 Jul 6;287(28):23451-63
references: 22610379
references: Am J Physiol Regul Integr Comp Physiol. 2005 Jul;289(1):R266-74
references: 15790751
references: Physiol Res. 2009;58(4):599-603
references: 19761352
references: Pflugers Arch. 2006 Sep;452(6):756-65
references: 16636868
references: J Biol Chem. 2010 Sep 10;285(37):28614-26
references: 20595385
references: Am J Physiol. 1998 Nov;275(5 Pt 2):F633-50
references: 9815123
references: J Biol Chem. 2013 Jan 11;288(2):1226-37
references: 23192345
references: Am J Physiol Regul Integr Comp Physiol. 2006 May;290(5):R1397-406
references: 16357096
references: Adv Space Biol Med. 2005;10:7-40
references: 16101103
references: Pflugers Arch. 2008 Aug;456(5):979-89
references: 18214523
references: Brain. 2001 Jun;124(Pt 6):1100-13
references: 11353726
references: Curr Opin Nephrol Hypertens. 2008 Sep;17(5):526-32
references: 18695395
references: Biophys J. 2002 Apr;82(4):1907-19
references: 11916849
references: J Gen Physiol. 2013 Oct;142(4):327-45
references: 24081980
references: Physiol Rev. 2003 Oct;83(4):1269-324
references: 14506306
references: Ann N Y Acad Sci. 2003 Apr;986:639-41
references: 12763908
references: Acta Physiol (Oxf). 2008 Sep;194(1):67-79
references: 18373741
references: Jpn J Physiol. 2002 Jun;52(3):235-45
references: 12230800
references: Brain. 2002 Jul;125(Pt 7):1510-21
references: 12077001
references: PLoS One. 2012;7(3):e33719
references: 22442718
references: J Appl Physiol (1985). 1990 Jan;68(1):1-12
references: 2179205
references: PLoS One. 2014;9(4):e96395
references: 24780915
references: Aviat Space Environ Med. 2001 May;72(5):471-6
references: 11346014
references: Biofizika. 2008 Nov-Dec;53(6):1051-7
references: 19137692
references: Trends Neurosci. 2001 Oct;24(10):602-8
references: 11576675
references: Am J Physiol Regul Integr Comp Physiol. 2001 Sep;281(3):R917-25
references: 11507009
references: PLoS One. 2014;9(10):e110514
references: 25310715
references: Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10
references: 15253893
references: Acta Physiol (Oxf). 2010 Apr;198(4):487-98
references: 19895607
references: J Muscle Res Cell Motil. 2008;29(6-8):221-30
references: 19130271
references: Biochem J. 1994 Dec 1;304 ( Pt 2):635-40
references: 7999001
references: Front Physiol. 2013 Oct 11;4:284
references: 24130531
references: Mol Cell Biochem. 2012 Aug;367(1-2):113-24
references: 22547201
references: Physiol Rev. 2000 Jul;80(3):1215-65
references: 10893434
references: J Cell Sci. 2001 Feb;114(Pt 4):751-62
references: 11171381
references: J Biol Chem. 1988 Jul 25;263(21):10436-42
references: 2839491
references: Biofizika. 2012 Sep-Oct;57(5):771-88
references: 23136769
references: Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2565-70
references: 19196986
references: J Physiol. 2007 Nov 1;584(Pt 3):983-95
references: 17855757
references: Exp Physiol. 2008 Dec;93(12):1249-62
references: 18586859
references: Physiol Rev. 2000 Oct;80(4):1411-81
references: 11015618