title: Bone Marrow work-up: report of a pilot study.
abstract: In this pilot study we changed several pre-analytical variables of bone marrow trephine biopsy processing with the task to achieve not only a preservation of morphology and antigens but also of nucleic acids. The changes involved employment of a newly established decalcification solution in conjunction with a short fixation time (2 h after receiving the specimens) and performance of decalcification at 37 °C. The comparison of the obtained results from three specimens with those of our routinely established protocol unequivocally revealed that the novel decalcification solution results in a superior preservation of nucleic acids, with only slight differences in preservation of morphology and cellular antigens. These encouraging results imply that this novel decalcification solution will allow a widely accepted standardization of bone marrow trephine biopsy processing.
authors: Institute for Pathology, Charité Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany, ioannis.anagnostopoulos@charite.de.
authors: Ioannis
authors: I
authors: Anagnostopoulos
authors: Dido
authors: D
authors: Lenze
authors: Michael
authors: M
authors: Hummel
authors: Manfred
authors: M
authors: Dietel
authors: Korinna
authors: K
authors: Jöhrens
mesh_terms: D001706:Biopsy / Q000379:methods; D001853:Bone Marrow / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D060965:Clonal Evolution; D004247:DNA / Q000302:isolation & purification / Q000592:standards; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000592:standards; D011786:Quality Control; D013048:Specimen Handling / Q000379:methods* / Q000592:standards
chemical_list: D007143:Immunoglobulin Heavy Chains; D004247:DNA