title: Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis.
abstract: Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson's diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.
authors: Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan.
authors: Noriko
authors: N
authors: Ido
authors: Kaori
authors: K
authors: Iwabuchi
authors: Yusuke
authors: Y
authors: Sato'o
authors: Yasuo
authors: Y
authors: Sato
authors: Masaru
authors: M
authors: Sugawara
authors: Gakuji
authors: G
authors: Yaegashi
authors: Masaru
authors: M
authors: Konno
authors: Masato
authors: M
authors: Akiba
authors: Kiyoshi
authors: K
authors: Tanaka
authors: Katsuhiko
authors: K
authors: Omoe
authors: Ikuo
authors: I
authors: Uchida
mesh_terms: D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D058885:Multilocus Sequence Typing / Q000379:methods / Q000662:veterinary*; D045483:Rivers / Q000382:microbiology*; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology
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