title: Brucella CβG induces a dual pro- and anti-inflammatory response leading to a transient neutrophil recruitment.
abstract: Brucella is the causing agent of a chronic zoonosis called brucellosis. The Brucella β-1,2 cyclic glucan (CβG) is a virulence factor, which has been described as a potent immune stimulator, albeit with no toxicity for cells and animals. We first used a genome-wide approach to characterize human myeloid dendritic cell (mDC) responses to CβG. Transcripts related to inflammation (IL-6, IL2RA, PTGS2), chemokine (CXCR7, CXCL2) and anti-inflammatory pathways (TNFAIP6, SOCS3) were highly expressed in CβG-treated mDC. In mouse GMCSF-derived DC, CβG triggered the expression of both activation (CXCL2, KC) and inhibition (SOCS3 and TNFAIP6) molecules. We then characterized the inflammatory infiltrates at the level of mouse ear when injected with CβG or LPS. CβG yielded a lower and transient recruitment of neutrophils compared to LPS. The consequence of these dual pro- and anti-inflammatory signals triggered by CβG corresponds to the induction of a controlled local inflammation.
authors: a Centre d'Immunologie de Marseille-Luminy (CIML) ; Aix-Marseille University ; Marseille , France.
authors: Clara
authors: C
authors: Degos
authors: Aurélie
authors: A
authors: Gagnaire
authors: Romain
authors: R
authors: Banchereau
authors: Ignacio
authors: I
authors: Moriyón
authors: Jean-Pierre
authors: JP
authors: Gorvel
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000276:immunology* / Q000472:pathogenicity; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002478:Cells, Cultured; D054426:Chemokine CXCL2 / Q000096:biosynthesis; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D004423:Ear / Q000502:physiology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D054387:Receptors, CXCR / Q000096:biosynthesis; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis; D047071:beta-Glucans / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules; D054426:Chemokine CXCL2; C501897:Cmkor1 protein, mouse; C404915:Cxcl2 protein, mouse; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D015850:Interleukin-6; D054387:Receptors, CXCR; C491411:Socs3 protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C513057:Tnfaip6 protein, mouse; D047071:beta-Glucans; C080135:cyclic beta(1-2)glucan; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: Brucella; CβG, Beta-1,2 cyclic glucan; DC, dendritic cells; E. coli, Escherichia coli; LPS, lipopolysaccharide; cyclic β glucan; inflammation; lipopolysaccharide; neutrophil
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