title: An adjuvanted, tetravalent dengue virus purified inactivated vaccine candidate induces long-lasting and protective antibody responses against dengue challenge in rhesus macaques.
abstract: The immunogenicity and protective efficacy of a candidate tetravalent dengue virus purified inactivated vaccine (TDENV PIV) formulated with alum or an Adjuvant System (AS01, AS03 tested at three different dose levels, or AS04) was evaluated in a 0, 1-month vaccination schedule in rhesus macaques. One month after dose 2, all adjuvanted formulations elicited robust and persisting neutralizing antibody titers against all four dengue virus serotypes. Most of the formulations tested prevented viremia after challenge, with the dengue serotype 1 and 2 virus strains administered at 40 and 32 weeks post-dose 2, respectively. This study shows that inactivated dengue vaccines, when formulated with alum or an Adjuvant System, are candidates for further development.
authors: Stefan
authors: S
authors: Fernandez
authors: Stephen J
authors: SJ
authors: Thomas
authors: Rafael
authors: R
authors: De La Barrera
authors: Rawiwan
authors: R
authors: Im-Erbsin
authors: Richard G
authors: RG
authors: Jarman
authors: Benoît
authors: B
authors: Baras
authors: Jean-François
authors: JF
authors: Toussaint
authors: Sally
authors: S
authors: Mossman
authors: Bruce L
authors: BL
authors: Innis
authors: Alexander
authors: A
authors: Schmidt
authors: Marie-Pierre
authors: MP
authors: Malice
authors: Pascale
authors: P
authors: Festraets
authors: Lucile
authors: L
authors: Warter
authors: J Robert
authors: JR
authors: Putnak
authors: Kenneth H
authors: KH
authors: Eckels
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002522:Chlorocebus aethiops; D003715:Dengue / Q000517:prevention & control* / Q000821:virology; D053059:Dengue Vaccines / Q000008:administration & dosage / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D008253:Macaca mulatta; D008297:Male; D012367:RNA, Viral / Q000097:blood; D011897:Random Allocation; D013997:Time Factors; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014709:Vero Cells; D014766:Viremia / Q000517:prevention & control / Q000821:virology
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D053059:Dengue Vaccines; D012367:RNA, Viral; D015164:Vaccines, Inactivated
references: Vaccine. 2011 Aug 11;29(35):5925-31
references: 21723353
references: J Virol. 2006 Oct;80(19):9577-85
references: 16973561
references: J Virol. 1990 Sep;64(9):4356-63
references: 2143542
references: Nature. 1977 Feb 24;265(5596):739-41
references: 404559
references: J Med Virol. 2008 Jan;80(1):134-46
references: 18041019
references: Yale J Biol Med. 1970 Apr;42(5):350-62
references: 5419208
references: J Infect Dis. 1973 Jul;128(1):15-22
references: 4198024
references: J Infect Dis. 2013 Mar 15;207(6):957-65
references: 23329850
references: Vaccine. 2008 Feb 6;26(6):817-28
references: 18191005
references: J Infect Dis. 2004 Mar 15;189(6):990-1000
references: 14999601
references: Expert Rev Vaccines. 2012 Mar;11(3):349-66
references: 22380826
references: Curr Opin Infect Dis. 2013 Jun;26(3):284-9
references: 23449140
references: Vaccine. 2011 Mar 16;29(13):2461-73
references: 21256188
references: Am J Trop Med Hyg. 2013 Jan;88(1):73-88
references: 23208878
references: Intervirology. 2001;44(2-3):176-97
references: 11509879
references: Clin Vaccine Immunol. 2011 Apr;18(4):523-32
references: 21270280
references: Lancet. 2012 Nov 3;380(9853):1559-67
references: 22975340
references: J Immunol. 2009 Nov 15;183(10):6186-97
references: 19864596
references: Curr Top Microbiol Immunol. 2010;338:129-43
references: 19802583
references: Front Immunol. 2014 May 06;5:195
references: 24834067
references: Vaccine. 2005 Aug 15;23(35):4442-52
references: 16005749
references: Virol J. 2007;4:23
references: 17328799
references: Curr Top Microbiol Immunol. 2010;338:83-98
references: 19802580
references: J Infect Dis. 2012 Aug 1;206(3):389-98
references: 22615312
references: Influenza Other Respir Viruses. 2008 Nov;2(6):251-60
references: 19453402
references: J Infect Dis. 1973 Jul;128(1):7-14
references: 4198027
references: J Infect Dis. 1996 Dec;174(6):1176-84
references: 8940206
references: Vaccine. 2010 Jan 8;28(3):849-57
references: 19835828
references: Vaccine. 2013 Jan 7;31(3):461-71
references: 23153447
references: Am J Trop Med Hyg. 2011 Jun;84(6):978-87
references: 21633037
references: Pediatr Infect Dis J. 2004 Feb;23(2):99-109
references: 14872173
references: Trop Med Int Health. 2011 Aug;16(8):936-48
references: 21624014
references: Nature. 2013 Apr 25;496(7446):504-7
references: 23563266
references: AIDS. 2013 Jan 2;27(1):87-93
references: 23018437
references: Vaccine. 2007 May 22;25(21):4130-9
references: 17428588
references: Expert Rev Vaccines. 2011 Apr;10(4):471-86
references: 21506645
references: J Virol. 2008 Jul;82(13):6631-43
references: 18448542
references: J Infect Dis. 2006 Jun 15;193(12):1658-65
references: 16703509
references: Vaccine. 2005 Nov 1;23(45):5205-11
references: 16055233
references: Clin Microbiol Rev. 1998 Jul;11(3):480-96
references: 9665979
references: J Infect Dis. 1988 Oct;158(4):876-80
references: 3171230
references: PLoS Negl Trop Dis. 2011 Aug;5(8):e1267
references: 21829748
references: Vaccine. 2013 Sep 23;31(41):4501-7
references: 23896423
references: Vaccine. 2011 Aug 26;29(37):6408-18
references: 21554915
references: Trop Med Health. 2011 Dec;39(4 Suppl):45-51
references: 22500136
references: J Virol Methods. 2008 Oct;153(1):1-6
references: 18652847
references: Curr Opin Microbiol. 2008 Aug;11(4):369-77
references: 18644250
references: Am J Trop Med Hyg. 2003 Dec;69(6 Suppl):12-6
references: 14740950
references: Vaccine. 2007 Apr 30;25(17):3445-53
references: 17241714
references: J Infect Dis. 2014 Jun 15;209 Suppl 2:S61-5
references: 24872398
references: J Infect Dis. 2008 Aug 15;198(4):493-9
references: 18588481
references: Expert Rev Vaccines. 2013 Aug;12(8):871-3
references: 23944374
references: Virology. 2010 Jan 20;396(2):280-8
references: 19913867
references: Science. 2010 May 7;328(5979):745-8
references: 20448183
references: J Virol. 2005 May;79(9):5516-28
references: 15827166
references: Nat Rev Microbiol. 2005 Jan;3(1):13-22
references: 15608696
references: Vaccine. 2010 Jan 8;28(3):632-49
references: 19808029