title: Identification and structural elucidation of steroidal saponins from the root of Paris polyphylla by HPLC-ESI-QTOF-MS/MS.
abstract: The root of Paris polyphylla (RPP) is widely used as a traditional Chinese medicine for a long time due to the good properties of heat-clearing and detoxicating, detumescence, sedation, acesodyne and haemostasis. To clarify on the bioactive substances and ensure the safety in clinical medication, a feasible and accurate strategy was developed by applying the high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). Separation was performed an Agilent poroshell 120 EC-C18 column (2.7 × 100 mm, i.d., 2.7 μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Based on the proposed strategy, 30 constituents, mainly including steroidal saponins, were characterised or tentatively identified, 2 of which were the first to be reported as the potential new steroidal saponins in RPP. In conclusion, the HPLC-ESI-QTOF-MS/MS is a feasible and credible technique to separate and identify steroidal saponins from botanical extracts.
authors: a Department of Pharmaceutical and Life Sciences , Jiujiang University , Jiujiang 332005 , People's Republic of China.
authors: Yun
authors: Y
authors: Ling
authors: Zhiwen
authors: Z
authors: Fu
authors: Qing
authors: Q
authors: Zhang
authors: Lingling
authors: L
authors: Xu
authors: Liang
authors: L
authors: Liao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D010840:Phytosterols; D010936:Plant Extracts; D012503:Saponins
keywords: HPLC-ESI-QTOF-MS/MS; Paris polyphylla; separation