title: The Pathological Response to Anthracycline is Associated with Topoisomerase IIα Gene Amplification in the HER2 Breast Cancer Subset.
abstract: BACKGROUND
  HER2-positive breast cancer sensitivity to anthracyclines is enhanced when topoisomerase IIα (TOP2A) is co-amplified under both adjuvant and metastatic settings. However, the relationship between anthracycline sensitivity and TOP2A amplification in HER2-positive breast cancers in neoadjuvant settings is not known.
  
  
  METHODS
  The TOP2A gene status was examined by FISH in biopsies from 18 patients who received anthracycline and cyclophosphamide before surgery.
  
  
  RESULTS
  The TOP2A gene was amplified in 6/17 patients and was significantly associated with pathological response to the chemotherapy regimen.
  
  
  CONCLUSIONS
  TOP2A amplification could predict anthracycline-sensitivity. Thus, the HER2/TOP2A co-amplified subtype may be effectively treated by anthracycline-containing regimens alone.
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Takashi
authors: T
authors: Ishikawa
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Takeshi
authors: T
authors: Sasaki
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Mikiko
authors: M
authors: Tanabe
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Kazutaka
authors: K
authors: Narui
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Kumiko
authors: K
authors: Kida
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Kazuhiro
authors: K
authors: Shimada
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Daisuke
authors: D
authors: Shimizu
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Akimitsu
authors: A
authors: Yamada
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Satoshi
authors: S
authors: Morita
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Mari S
authors: MS
authors: Oba
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Kae
authors: K
authors: Kawachi
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Akinori
authors: A
authors: Nozawa
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Yasushi
authors: Y
authors: Ichikawa
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Kazuaki
authors: K
authors: Takabe
authors: Department of Breast Surgery, Tokyo Medical University (TI); Departments of Breast and Thyroid Surgery (TI, KN, KK, KS, DS, AY), Pathology (TS, MT, KK) and Biostatistics and Epidemiology (SM, MSO), Yokohama City University Medical Center, Yokohama, Japan; Department of Gastrointestinal Surgery and Clinical Oncology, Yokohama City University, Yokohama, Japan (YI, IE); and Division of Surgical Oncology, Department of Surgery, Virginia Commonwealth University School of Medicine and Massey Cancer Center, Richmond, VA, USA (KT).
authors: Itaru
authors: I
authors: Endo
keywords: Breast Cancer; FISH; HER2; Neoadjuvant; Predictive factor; TOP2A
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