title: [Detection of Bcl-2 in human hepatocellular carcinoma and down-regulation with siRNA].
abstract: OBJECTIVE
  To investigate the relationship between the level of Bcl-2 in human hepatocellular carcinoma (HCC) tissues and the clinicopathological characteristics and prognosis of patients, and to detect the proliferation of SMMC-7721 cells after Bcl-2 is down-regulated with siRNA.
  
  
  METHODS
  The level of Bcl-2 in 68 HCC tissues was detected by reverse transcription PCR, SP immunohistochemistry and Western blotting. The 68 HCC tissues were divided into two groups based on the level of Bcl-2. The clinicopathological characteristics and prognosis of the patients were compared between groups. The proliferation of SMMC-7721 cells was evaluated by MTT assay after Bcl-2 was inhibited with siRNA.
  
  
  RESULTS
  Compared with corresponding noncancerous liver tissues, the level of Bcl-2 was significantly elevated in HCC tissues. The level of Bcl-2 was correlated with clinicopathological characteristics, and the patient with higher expression level of Bcl-2 had relatively poorer prognosis. Down-regulation of Bcl-2 expression by siRNA significantly inhibited the proliferation of SMMC-7721 cells.
  
  
  CONCLUSIONS
  The level of Bcl-2 is abnormally elevated in HCC tissues and can serve as a predictor of the prognosis of patients with HCC. The down-regulated of Bcl-2 in SMMC-7721 cells can inhibit the proliferation of cancer cells.
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Zhikui
authors: Z
authors: Liu
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Tao
authors: T
authors: Song
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Changwei
authors: C
authors: Dou
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Xin
authors: X
authors: Zheng
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Yingmin
authors: Y
authors: Yao
authors: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors: Qingguang
authors: Q
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D017207:Rats, Sprague-Dawley
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering