title: Cytosolic irradiation of femtosecond laser induces mitochondria-dependent apoptosis-like cell death via intrinsic reactive oxygen cascades.
abstract: High-intensity femtosecond lasers have recently been used to irreversibly disrupt nanoscale structures, such as intracellular organelles, and to modify biological functions in a reversible manner: so-called nanosurgery and biophotomodulation. Femtosecond laser pulses above the threshold intensity sufficient for reversible biophotomodulation can cause irreversible changes in the irradiated cell, eventually leading to cell death. Here, we demonstrated that cytosolic irradiation with a femtosecond laser produced intrinsic cascades of reactive oxygen species (ROS), which led to rapid apoptosis-like cell death via a caspase and poly (ADP-ribose) polymerase 1 (PARP-1) signaling pathway. We further showed that cells with enhanced mitochondrial fusion activity are more resilient to laser-induced stress compared to those with enforced mitochondrial fission. Taken together, these findings provide fundamental insight into how optical stimulation intervenes in intrinsic cellular signaling pathways and functions.
authors: 1] Department of Bio and Brain Engineering, KAIST, Daejeon, Korea [2] KAIST Institute for Optical Science and Technology, KAIST, Daejeon, Korea.
authors: Jonghee
authors: J
authors: Yoon
authors: 1] Department of Bio and Brain Engineering, KAIST, Daejeon, Korea [2] KAIST Institute for the BioCentury, KAIST, Daejeon, Korea.
authors: Seung-Wook
authors: SW
authors: Ryu
authors: Department of Bio and Brain Engineering, KAIST, Daejeon, Korea.
authors: Seunghee
authors: S
authors: Lee
authors: 1] Department of Bio and Brain Engineering, KAIST, Daejeon, Korea [2] KAIST Institute for Optical Science and Technology, KAIST, Daejeon, Korea [3] KAIST Institute for the BioCentury, KAIST, Daejeon, Korea.
authors: Chulhee
authors: C
authors: Choi
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects*; D063154:Mitochondrial Dynamics / Q000528:radiation effects; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000528:radiation effects
chemical_list: D017382:Reactive Oxygen Species; D011065:Poly(ADP-ribose) Polymerases; D002118:Calcium
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