title: Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass.
abstract: Lactic acid bacteria (LAB) are important for the processing of various food products. Although genetically modified organisms have contributed to improvements in various food products, there are some limitations. Thus, the discovery of wild strains from natural sources must be considered as the most suitable approach for identifying new LAB. Therefore, we planned to isolate and characterise the LAB from Italian ryegrass forage and evaluate their biological potential.
  
  
  
  A total of 28 strains were isolated and screened for their anti-fungal and probiotic properties. A single strain was selected due to its antifungal and probiotic efficiency. The strain was identified as Pediococcus pentosaceus KCC-23. The strain KCC-23 showed effective inhibition against Aspergillus fumigatus, Pencillium chrysogenum, Pencillium roqueforti, Botrytis elliptica and Fusarium oxysporum. Further, it survived low pH, and the presence of bile salts and gastric juice. It exhibited significant aggregation and hydrophobicity properties. The KCC-23 effectively assimilated cholesterol and had the ability to utilise pre-biotics such as raffinose and inulin. Finally, KCC-23 exhibited significant free radical scavenging activity.
  
  
  
  P. pentosaceus KCC-23 showed effective anti-fungal, probiotic and anti-oxidant properties and would be a promising isolate for exploitation in the formulation of food for ruminants and humans.
authors: Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
authors: Soundharrajan
authors: S
authors: Ilavenil
authors: Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
authors: Mayakrishnan
authors: M
authors: Vijayakumar
authors: The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi, Japan.
authors: Da Hye
authors: DH
authors: Kim
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
authors: Mariadhas
authors: M
authors: Valan Arasu
authors: Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
authors: Hyung Su
authors: HS
authors: Park
authors: Department of Biotechnology, PRIST University, Thanjavur, Tamilnadu, 613-403, India.
authors: Sivanesan
authors: S
authors: Ravikumar
authors: Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
authors: Ki Choon
authors: KC
authors: Choi
mesh_terms: D000924:Anticholesteremic Agents; D000975:Antioxidants; D001647:Bile Acids and Salts / Q000494:pharmacology; D005285:Fermentation; D005659:Fungicides, Industrial; D005750:Gastric Juice; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000378:metabolism; D007558:Italy; D008129:Lolium / Q000382:microbiology*; D010376:Pediococcus / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D056692:Prebiotics; D019936:Probiotics; D011887:Raffinose / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D000975:Antioxidants; D001647:Bile Acids and Salts; D005659:Fungicides, Industrial; D056692:Prebiotics; D007444:Inulin; D011887:Raffinose
keywords: Italian ryegrass; LAB; P. pentosaceus KCC-23; antifungal; antioxidant; probiotic