title: Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China.
abstract: In this study, we attenuated a Chinese QX-like nephropathogenic infectious bronchitis virus (IBV) strain, YX10, by passaging through fertilized chicken eggs. The 90th passage strain (YX10p90) was selected as the live-attenuated vaccine candidate strain. YX10p90 was found to be safe in 7-day-old specific pathogen free chickens without induction of morbidity or mortality. YX10p90 provided nearly complete protection against QX-like (CH I genotype) strains and partial protection against other two major Chinese genotype strains. YX10p90 also showed no reversion to virulence after five back passages in chickens. An IBV polyvalent vaccine containing YX10p90 was developed and showed that it could provide better protection against major Chinese IBV virulent strains than commercial polyvalent vaccines. In addition, the complete genome sequence of YX10p90 was sequenced. Multiple-sequence alignments identified 38 nucleotide substitutions in the whole genome which resulted in 26 amino acid substitutions and a 110-bp deletion in the 3' untranslated region. In conclusion, the attenuated YX10p90 strain exhibited a fine balance between attenuation and immunogenicity, and should be considered as a candidate vaccine to prevent infection of Chinese QX-like nephropathogenic IBV.
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China.
authors: Keyu
authors: K
authors: Feng
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Wen's Foodstuff Group Co., Ltd., Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, PR China.
authors: Yu
authors: Y
authors: Xue
authors: Langfang Academy of Agriculture and Forestry Sciences, Langfang 065000, PR China.
authors: Jinglan
authors: J
authors: Wang
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China.
authors: Weiguo
authors: W
authors: Chen
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China.
authors: Feng
authors: F
authors: Chen
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China.
authors: Yingzuo
authors: Y
authors: Bi
authors: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, PR China. Electronic address: qmx@scau.edu.cn.
authors: Qingmei
authors: Q
authors: Xie
mesh_terms: D020413:3' Untranslated Regions; D019943:Amino Acid Substitution; D000818:Animals; D002645:Chickens; D002681:China; D018352:Coronavirus Infections / Q000453:epidemiology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D056738:Cross Protection; D064789:Immunity, Heterologous; D001351:Infectious bronchitis virus / Q000276:immunology*; D020125:Mutation, Missense; D017354:Point Mutation; D011201:Poultry Diseases / Q000453:epidemiology* / Q000517:prevention & control* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D012692:Serial Passage; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology* / Q000302:isolation & purification*; D014774:Virulence
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Infectious bronchitis virus; Live-attenuated vaccine strain; Nephropathogenic; QX-like
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