title: Effect of interleukin-17 on in vitro cytokine production in healthy controls and patients with severe sepsis.
abstract: OBJECTIVE
  Interleukin (IL)-17 family members (IL-17A to IL-17F) are appearing to play key roles in host defense and inflammatory disease. Recently, several cytokines, such as IL-6, IL-10, IL-12, and transforming growth factor (TGF)-β1, were shown to have vital roles in severe sepsis. However, the influence of IL-17 on these cytokine responses from peripheral blood mononuclear cells (PBMCs) is unclear.
  
  
  METHODS
  Fifty-two patients who were admitted to our intensive care unit (ICU) because of severe sepsis were enrolled into this study. To validate experimental findings, 25 healthy controls were enrolled. Lipopolysaccharide-stimulated PBMCs with IL-17 or anti-IL-17 treatments were cultured for 24 hours. IL-6, IL-10, IL-12, and TGF-β1 levels in supernatants were measured.
  
  
  RESULTS
  The IL-12 production from stimulated PBMCs was increased after IL-17 treatment in both control and patient groups. Additional treatment of anti-IL-17 enhanced IL-10 production but decreased IL-12 production from stimulated PBMCs of healthy controls and patients with severe sepsis.
  
  
  CONCLUSIONS
  IL-17 was helpful for inflammation in severe sepsis. Lack of IL-17 decreased IL-12 and enhanced IL-10 production from PBMCs, which resulted in immune imbalance.
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors: Huang-Pin
authors: HP
authors: Wu
authors: Department of Emergence, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors: Chi-Chung
authors: CC
authors: Shih
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors: Chien-Ming
authors: CM
authors: Chu
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors: Chih-Yu
authors: CY
authors: Huang
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors: Chung-Ching
authors: CC
authors: Hua
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors: Yu-Chih
authors: YC
authors: Liu
authors: Department of Chemistry, National Chung-Hsing University, Taichung, Taiwan. Electronic address: chuang@nchu.edu.tw.
authors: Duen-Yau
authors: DY
authors: Chuang
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism; D020381:Interleukin-17 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000276:immunology*; D013624:Taiwan; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; C508600:IL6 protein, human; D020381:Interleukin-17; D015850:Interleukin-6; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D018664:Interleukin-12
keywords: cytokine response; interleukin-17; severe sepsis