title: Evaluation of Ebola virus inactivation procedures for Plasmodium falciparum malaria diagnostics.
abstract: Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. We compared the performances of a rapid diagnostic test (BinaxNOW) and real-time PCR with P. falciparum-positive specimens before and after heat and Triton X-100 inactivation, and we documented no loss of sensitivity.
authors: Public Health Ontario Laboratories, Public Health Ontario, Toronto, Ontario, Canada.
authors: Rachel
authors: R
authors: Lau
authors: Public Health Ontario Laboratories, Public Health Ontario, Toronto, Ontario, Canada.
authors: Amanda
authors: A
authors: Wang
authors: Public Health Ontario Laboratories, Public Health Ontario, Toronto, Ontario, Canada.
authors: Ann
authors: A
authors: Chong-Kit
authors: Public Health Ontario Laboratories, Public Health Ontario, Toronto, Ontario, Canada.
authors: Filip
authors: F
authors: Ralevski
authors: Public Health Ontario Laboratories, Public Health Ontario, Toronto, Ontario, Canada Tropical Disease Unit, Division of Infectious Diseases, UHN-Toronto General Hospital, Toronto, Ontario, Canada Department of Medicine, University of Toronto, Toronto, Ontario, Canada andrea.boggild@utoronto.ca.
authors: Andrea K
authors: AK
authors: Boggild
mesh_terms: D029043:Ebolavirus / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis* / Q000821:virology; D006358:Hot Temperature; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D017830:Octoxynol; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D038301:Virus Inactivation
chemical_list: D011933:Reagent Kits, Diagnostic; D017830:Octoxynol
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