title: Anti-desmocollin autoantibodies in nonclassical pemphigus.
abstract: BACKGROUND
  Despite the established pathogenic role of anti-desmoglein (Dsg) antibodies in classical pemphigus, the significance of autoantibodies to another desmosomal cadherin, desmocollin (Dsc) is at present unknown. No consistent immunoassay for immunoglobulin (Ig) G autoantibodies to Dscs has been developed.
  
  
  OBJECTIVE
  The aim of this study was to develop reliable assays to detect anti-Dsc autoantibodies.
  
  
  METHODS
  We expressed soluble recombinant proteins (RPs) of human Dsc1-3 in mammalian cells and examined sera of various types of pemphigus, including 79 paraneoplastic pemphigus (PNP) sera, by novel enzyme-linked immunosorbent assays (ELISAs) using the RPs. We also performed ELISAs of Dsc baculoproteins and used the complementary DNA (cDNA) transfection method, and compared the results with those of mammalian ELISAs.
  
  
  RESULTS
  Through mammalian ELISAs, IgG autoantibodies to Dsc1, Dsc2 and Dsc3 were detected in 16.5%, 36.7% and 59.5% of PNP sera, respectively, and considerable numbers of pemphigus herpetiformis (PH) and pemphigus vegetans (PVeg) sera reacted strongly with Dsc1 and Dsc3. Mammalian ELISAs were highly specific and more sensitive than baculoprotein ELISAs or the cDNA transfection method. Several Dsc-positive sera, particularly PH sera, showed no reactivity with Dsgs. The reactivity of PNP serum and PVeg serum with Dscs was not abolished by pre-absorption with Dsg RPs.
  
  
  CONCLUSIONS
  The results of these novel ELISAs indicated that IgG anti-Dsc autoantibodies were frequently detected and potentially pathogenic in nonclassical pemphigus.
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: N
authors: N
authors: Ishii
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: K
authors: K
authors: Teye
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: S
authors: S
authors: Fukuda
authors: Intellectual Property and Clinical Development Department/Business Development Division, Medical & Biological Laboratories Co., Ltd, Nagoya, Japan.
authors: R
authors: R
authors: Uehara
authors: Antibody Engineering Department/Manufacturing Division, Medical & Biological Laboratories Co., Ltd, Nagoya, Japan.
authors: T
authors: T
authors: Hachiya
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: H
authors: H
authors: Koga
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: A
authors: A
authors: Tsuchisaka
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: S
authors: S
authors: Numata
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: B
authors: B
authors: Ohyama
authors: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: C
authors: C
authors: Tateishi
authors: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: D
authors: D
authors: Tsuruta
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: M
authors: M
authors: Furumura
authors: Biostatistics Center, Kurume University, Kurume, Japan.
authors: S
authors: S
authors: Hattori
authors: Department of Dermatology, St Marianna University School of Medicine, Kawasaki, Japan.
authors: T
authors: T
authors: Kawakami
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: C
authors: C
authors: Ohata
authors: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan.
authors: T
authors: T
authors: Hashimoto
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D018076:DNA, Complementary / Q000032:analysis; D051187:Desmocollins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D047468:Immunoprecipitation / Q000379:methods; D010392:Pemphigus / Q000276:immunology*; D012372:ROC Curve; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D001323:Autoantibodies; D018076:DNA, Complementary; D051187:Desmocollins; D007074:Immunoglobulin G; D011994:Recombinant Proteins