title: A comparative analysis of methylome profiles of Campylobacter jejuni sheep abortion isolate and gastroenteric strains using PacBio data.
abstract: Campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the United States. The recent emergence of a highly virulent, tetracycline-resistant C. jejuni subsp. jejuni sheep abortion clone (clone SA) in the United States, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. Pacific Biosciences' Single Molecule, Real-Time sequencing technology was used to explore the variation in the genome-wide methylation patterns of the abortifacient clone SA (IA3902) and phenotypically distinct gastrointestinal-specific C. jejuni strains (NCTC 11168 and 81-176). Several notable differences were discovered that distinguished the methylome of IA3902 from that of 11168 and 81-176: identification of motifs novel to IA3902, genome-specific hypo- and hypermethylated regions, strain level variability in genes methylated, and differences in the types of methylation motifs present in each strain. These observations suggest a possible role of methylation in the contrasting disease presentations of these three C. jejuni strains. In addition, the methylation profiles between IA3902 and a luxS mutant were explored to determine if variations in methylation patterns could be identified that might explain the role of LuxS-dependent methyl recycling in IA3902 abortifacient potential.
authors: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University Ames, IA, USA.
authors: Kathy T
authors: KT
authors: Mou
authors: Genome Informatics Facility, Office of Biotechnology, Iowa State University Ames, IA, USA.
authors: Usha K
authors: UK
authors: Muppirala
authors: Genome Informatics Facility, Office of Biotechnology, Iowa State University Ames, IA, USA.
authors: Andrew J
authors: AJ
authors: Severin
authors: Pacific Biosciences Menlo Park, CA, USA.
authors: Tyson A
authors: TA
authors: Clark
authors: Pacific Biosciences Menlo Park, CA, USA.
authors: Matthew
authors: M
authors: Boitano
authors: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University Ames, IA, USA.
authors: Paul J
authors: PJ
authors: Plummer
keywords: Campylobacter jejuni; LuxS; methylation; methylome; quorum sensing
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