title: HIV DNA loads, plasma residual viraemia and risk of virological rebound in heavily treated, virologically suppressed HIV-infected patients.
abstract: In this single-centre, retrospective study, we analyzed data of 194 patients receiving antiretroviral therapy with <50 human immunodeficiency virus (HIV) RNA copies/mL in plasma and 318 HIV RNA/DNA paired samples. By kinetic polymerase chain reaction (kPCR) molecular system analysis, 104 (54%) subjects had undetectable HIV RNA and 90 (46%) had residual viraemia. Median (interquartile range) HIV DNA load was 780 (380-1930) copies/10(6) peripheral blood lymphocytes (PBL), and HIV DNA loads were independently associated with residual viraemia (p 0.002). Virological rebound occurred in 29/194 (15%) patients over a median (interquartile range) follow-up of 17.5 (13.5-31.5) months. Residual viraemia (p 0.002), but not HIV DNA load, was independently associated with virological rebound.
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy. Electronic address: nicola.gianotti@hsr.it.
authors: N
authors: N
authors: Gianotti
authors: Microbiology and Virology, San Raffaele Scientific Institute, Milan, Italy; Dipartimento di Biotecnologie e Scienze della Vita, University of Insubria, Varese, Italy.
authors: F
authors: F
authors: Canducci
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: L
authors: L
authors: Galli
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: F
authors: F
authors: Cossarini
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: S
authors: S
authors: Salpietro
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: A
authors: A
authors: Poli
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: S
authors: S
authors: Nozza
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: V
authors: V
authors: Spagnuolo
authors: Microbiology and Virology, San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, Italy.
authors: M
authors: M
authors: Clementi
authors: Microbiology and Virology, San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, Italy.
authors: M
authors: M
authors: Sampaolo
authors: Microbiology and Virology, San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, Italy.
authors: E R
authors: ER
authors: Ceresola
authors: Microbiology and Virology, San Raffaele Scientific Institute, Milan, Italy.
authors: S
authors: S
authors: Racca
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, Italy.
authors: A
authors: A
authors: Lazzarin
authors: Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors: A
authors: A
authors: Castagna
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood; D012008:Recurrence; D012189:Retrospective Studies; D019562:Viral Load; D014766:Viremia / Q000188:drug therapy* / Q000453:epidemiology / Q000821:virology*
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral
keywords: HIV-1 DNA load; kPCR; residual viraemia; virological failure; virological rebound