title: Investigation of killer cell immunoglobulin-like receptors KIR2DL2 and KIR2DL3 diversity and identification of ten novel KIR2DL3 alleles in the Chinese Han population.
abstract: KIR2DL2 and KIR2DL3 are important inhibitory receptors that recognize a subset of HLA-C allelic products carrying Ser77 and Asn80. In this study, we have determined KIR2DL2 and KIR2DL3 diversity in the Chinese Han population by a PCR sequence-based typing. Based on sequencing, the coding regions of 166 Chinese Han individuals, seven new polymorphic sites (238G>A, 405G>A, 476A>G, 550G>A, 608G>A, 789T>C, 947T>C) were found. KIR2DL2*00301, *00101, KIR2DL3*00101,*00201,*013, *015 and ten new KIR2DL3 variants (KIR2DL3*00105, 00106, 00107, 00108, 019, 020, 021, 022, 023 and 024) were identified, of which KIR2DL3*00101 was the most frequent allele. Compared with the sequences of KIR2DL3*00101, all sequences of 2DL3*00105, 2DL3*00106, 2DL3*00107 and 2DL3*00108 had one nucleotide substitution(789T>C, 261C>T, 489G>A and 405G>A),but none resulted in amino acid change. An A>G substitution was observed in nucleotide position 476 in 2DL3*019, 608 G>A in 2DL3*020, 824T>C in 2DL3*021 and 238 G>A in 2DL3*023. In addition, 2DL3*022 probably arose from 2DL3*00201 with a nucleotide substitution G>A at 550. There were more HLA-C1 positive individuals than HLA-C2. In conclusion, the data of allelic polymorphism for KIR2DL2 and KIR2DL3 were obtained in the Chinese Han population and ten novel KIR2DL3 alleles were identified.
authors: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China ; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors: Y
authors: Y
authors: He
authors: S
authors: S
authors: Tao
authors: Y
authors: Y
authors: Ying
authors: J
authors: J
authors: He
authors: F
authors: F
authors: Zhu
authors: H
authors: H
authors: Lv
mesh_terms: D000483:Alleles; D044466:Asian People; D004247:DNA / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation / Q000276:immunology; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000276:immunology; D054340:Receptors, KIR / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D015236:HLA-C Antigens; D054340:Receptors, KIR; D004247:DNA