title: Phosphorescent quantum dots/ethidium bromide nanohybrids based on photoinduced electron transfer for DNA detection.
abstract: Mercaptopropionic acid-capped Mn-doped ZnS quantum dots/ethidium bromide (EB) nanohybrids were constructed for photoinduced electron transfer (PIET) and then used as a room-temperature phosphorescence (RTP) probe for DNA detection. EB could quench the RTP of Mn-doped ZnS QDs by PIET, thereby forming Mn-doped ZnS QDs/EB nanohybrids and storing RTP. Meanwhile, EB could be inserted into DNA and EB could be competitively desorbed from the surface of Mn-doped ZnS QDs by DNA, thereby releasing the RTP of Mn-doped ZnS QDs. Based on this mechanism, a RTP sensor for DNA detection was developed. Under optimal conditions, the detection limit for DNA was 0.045 mg L(-1), the relative standard deviation was 1.7%, and the method linear ranged from 0.2 to 20 mg L(-1). The proposed method was applied to biological fluids, in which satisfactory results were obtained.
authors: School of Life and Technology, Changchun University of Science and Technology, Changchun 130022, PR China.
authors: Lin
authors: L
authors: Bi
authors: School of Life and Technology, Changchun University of Science and Technology, Changchun 130022, PR China. Electronic address: yuyuanhua888@126.com.
authors: Yuan-Hua
authors: YH
authors: Yu
mesh_terms: D004247:DNA / Q000032:analysis / Q000652:urine*; D004579:Electron Transport; D004996:Ethidium / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D049408:Luminescent Agents; D004247:DNA; D004996:Ethidium
keywords: DNA; Nanohybrids; Photoinduced electron transfer; Room-temperature phosphorescence