title: Local adaptations in bryophytes revisited: the genetic structure of the calcium-tolerant peatmoss Sphagnum warnstorfii along geographic and pH gradients.
abstract: Bryophytes dominate some ecosystems despite their extraordinary sensitivity to habitat quality. Nevertheless, some species behave differently across various regions. The existence of local adaptations is questioned by a high dispersal ability, which is thought to redistribute genetic variability among populations. Although Sphagnum warnstorfii is an important ecosystem engineer in fen peatlands, the causes of its rather wide niche along the pH/calcium gradient are poorly understood. Here, we studied the genetic variability of its global populations, with a detailed focus on the wide pH/calcium gradient in Central Europe. Principal coordinates analysis of 12 polymorphic microsatellite loci revealed a significant gradient coinciding with water pH, but independent of geography; even samples from the same fens were clearly separated along this gradient. However, most of the genetic variations remained unexplained, possibly because of the introgression from phylogenetically allied species. This explanation is supported by the small heterogeneous cluster of samples that appeared when populations morphologically transitional to S. subnites, S. rubellum, or S. russowii were included into the analysis. Alternatively, this unexplained variation might be attributed to a legacy of glacial refugia with recently dissolved ecological and biogeographic consequences. Isolation by distance appeared at the smallest scale only (up to 43 km). Negative spatial correlations occurred more frequently, mainly at long distances (up to 950 km), implying a genetic similarity among samples which are very distant geographically. Our results confirm the high dispersal ability of peatmosses, but simultaneously suggested that their ability to cope with a high pH/calcium level is at least partially determined genetically, perhaps via specific physiological mechanisms or a hummock-forming ability.
authors: Department of Botany and Zoology, Faculty of Science, Masaryk University Kotlářská 2, Brno, CZ 61137, Czech Republic.
authors: Eva
authors: E
authors: Mikulášková
authors: Department of Botany and Zoology, Faculty of Science, Masaryk University Kotlářská 2, Brno, CZ 61137, Czech Republic ; Department of Vegetation Ecology, Institute of Botany, Academy of Sciences of the Czech Republic Lidická 25/27, Brno, CZ 65720, Czech Republic.
authors: Michal
authors: M
authors: Hájek
authors: Department of Botany and Zoology, Faculty of Science, Masaryk University Kotlářská 2, Brno, CZ 61137, Czech Republic.
authors: Adam
authors: A
authors: Veleba
authors: Plant Science and Conservation, Chicago Botanic Gardens 1000 Lake Cook Road, Glencoe, IL, 60022, USA.
authors: Matthew G
authors: MG
authors: Johnson
authors: Department of Functional Ecology, Institute of Botany, Academy of Sciences of the Czech Republic Dukelská 135, Třeboň, CZ 37982, Czech Republic.
authors: Tomáš
authors: T
authors: Hájek
authors: Department of Biology, Duke University Durham, NC, 27708, USA.
authors: Jonathan A
authors: JA
authors: Shaw
keywords: Calcium tolerance; Sphagnum warnstorfii; ecotypic adaptation; hybridization; microsatellites; population structure
references: Mol Phylogenet Evol. 2009 Oct;53(1):277-86
references: 19539771
references: Ecology. 2010 Oct;91(10):3047-56
references: 21058564
references: Mol Ecol. 2011 Nov;20(22):4631-42
references: 21999331
references: Mol Ecol. 2004 Aug;13(8):2109-19
references: 15245387
references: New Phytol. 2012 Mar;193(4):1088-97
references: 22188609
references: Bioinformatics. 2007 Jul 15;23(14):1801-6
references: 17485429
references: Mol Ecol. 2011 Feb;20(4):753-67
references: 21199037
references: Science. 2006 May 19;312(5776):1015
references: 16709777
references: Evolution. 2011 Apr;65(4):1181-94
references: 21073451
references: Genetics. 2000 Jun;155(2):945-59
references: 10835412
references: New Phytol. 2010 Feb;185(3):852-64
references: 20002317
references: Mol Ecol. 2007 Jan;16(2):305-12
references: 17217346
references: Am J Bot. 2010 Oct;97(10):1707-18
references: 21616804
references: Mol Ecol. 2011 Aug;20(15):3202-18
references: 21722226
references: Mol Ecol. 2013 Dec;22(24):5983-99
references: 24128305
references: New Phytol. 2010 Mar;185(4):886-9; author reply 889-91
references: 20356344
references: Bioinformatics. 2012 Oct 1;28(19):2537-9
references: 22820204
references: Am J Bot. 2008 Dec;95(12):1606-20
references: 21628167
references: Mol Ecol. 2006 Aug;15(9):2305-15
references: 16842407
references: Mol Ecol. 2008 Dec;17(24):5364-77
references: 19121003
references: Mol Ecol. 2007 Feb;16(4):811-8
references: 17284213
references: Mol Ecol. 2012 Nov;21(22):5461-72
references: 23062192
references: Mol Phylogenet Evol. 2010 Apr;55(1):293-304
references: 19919850
references: Mol Ecol. 2005 Jul;14(8):2611-20
references: 15969739
references: Am J Bot. 2005 Nov;92(11):1774-83
references: 21646095
references: Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):637-42
references: 16407098
references: Heredity (Edinb). 2001 Oct;87(Pt 4):485-96
references: 11737298
references: Heredity (Edinb). 1999 Apr;82 Pt 4:391-400
references: 10383657
references: Am J Bot. 1999 Dec;86(12):1687-98
references: 10602762
references: Mol Ecol. 2009 Apr;18(7):1439-54
references: 19368647
references: Ecology. 2012 May;93(5):1106-14
references: 22764496