title: Protein-peptide arrays for detection of specific anti-hepatitis D virus (HDV) genotype 1, 6, and 8 antibodies among HDV-infected patients by surface plasmon resonance imaging.
abstract: Liver diseases linked to hepatitis B-hepatitis D virus co- or superinfections are more severe than those during hepatitis B virus (HBV) monoinfection. The diagnosis of hepatitis D virus (HDV) infection therefore remains crucial in monitoring patients but is often overlooked. To integrate HDV markers into high-throughput viral hepatitis diagnostics, we studied the binding of anti-HDV antibodies (Abs) using surface plasmon resonance imaging (SPRi). We focused on the ubiquitous HDV genotype 1 (HDV1) and the more uncommon African-HDV6 and HDV8 genotypes to define an array with recombinant proteins or peptides. Full-length and truncated small hepatitis D antigen (S-HDAg) recombinant proteins of HDV genotype 1 (HDV1) and 11 HDV peptides of HDV1, 6, and 8, representing various portions of the delta antigen were grafted onto biochips, allowing SPRi measurements to be made. Sixteen to 17 serum samples from patients infected with different HDV genotypes were injected onto protein and peptide chips. In all, Abs against HDV proteins and/or peptides were detected in 16 out of 17 infected patients (94.12%), although the amplitude of the SPR signal varied. The amino-terminal part of the protein was poorly immunogenic, while epitope 65-80, exposed on the viral ribonucleoprotein, may be immunodominant, as 9 patient samples led to a specific SPR signal on peptide 65 type 1 (65#1), independently of the infecting genotype. In this pilot study, we confirmed that HDV infection screening based on the reactivity of patient Abs against carefully chosen HDV peptides and/or proteins can be included in a syndrome-based viral hepatitis diagnostic assay. The preliminary results indicated that SPRi studying direct physical HDAg-anti-HDV Ab interactions was more convenient using linear peptide epitopes than full-length S-HDAg proteins, due to the regeneration process, and may represent an innovative approach for a hepatitis syndrome-viral etiology-exploring array.
authors: INSERM, U823, Grenoble, France Université Grenoble Alpes, Institut Albert Bonniot, Grenoble, France.
authors: Marie-Bernadette
authors: MB
authors: Villiers
authors: Centre National de Référence des Entérovirus, EA Site Laennec, Université Lyon 1, Lyon, France.
authors: Jean-Claude
authors: JC
authors: Cortay
authors: INSERM, U823, Grenoble, France Université Grenoble Alpes, Institut Albert Bonniot, Grenoble, France.
authors: Sandra
authors: S
authors: Cortès
authors: Centre National de Référence des Entérovirus, EA Site Laennec, Université Lyon 1, Lyon, France INSERM, U1052, UMR CNRS 5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France.
authors: Bénédicte
authors: B
authors: Bloquel
authors: Service de Bactériologie, Virologie-Hygiène, Hôpital Avicenne, Groupe des Hôpitaux Universitaires Paris Seine Saint-Denis, Assistance Publique-Hôpitaux de Paris, Laboratoire Associé au Centre National de Référence des Hépatites B, C et delta, Bobigny, France Université Paris 13/Sorbonne Paris Cité, UFR Santé Médecine Biologie Humaine, Bobigny, France.
authors: Ségolène
authors: S
authors: Brichler
authors: INSERM, U823, Grenoble, France Université Grenoble Alpes, Institut Albert Bonniot, Grenoble, France.
authors: Carine
authors: C
authors: Brakha
authors: INSERM, U1052, UMR CNRS 5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France.
authors: Alan
authors: A
authors: Kay
authors: Centre National de Référence des Entérovirus, EA Site Laennec, Université Lyon 1, Lyon, France.
authors: Nisrine
authors: N
authors: Falah
authors: INSERM, U1052, UMR CNRS 5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France Hospices Civils Lyon, Lyon, France Université de Lyon 1, Lyon, France.
authors: Fabien
authors: F
authors: Zoulim
authors: Université de Lyon 1, Lyon, France Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires (GEMBAS), Université Lyon 1, CNRS 5246 ICBMS, Villeurbanne, Cedex, France.
authors: Christophe
authors: C
authors: Marquette
authors: INSERM, U823, Grenoble, France Université Grenoble Alpes, Institut Albert Bonniot, Grenoble, France patrice.marche@inserm.fr paul.deny@inserm.fr.
authors: Patrice N
authors: PN
authors: Marche
authors: INSERM, U1052, UMR CNRS 5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France Service de Bactériologie, Virologie-Hygiène, Hôpital Avicenne, Groupe des Hôpitaux Universitaires Paris Seine Saint-Denis, Assistance Publique-Hôpitaux de Paris, Laboratoire Associé au Centre National de Référence des Hépatites B, C et delta, Bobigny, France Université Paris 13/Sorbonne Paris Cité, UFR Santé Médecine Biologie Humaine, Bobigny, France patrice.marche@inserm.fr paul.deny@inserm.fr.
authors: Paul
authors: P
authors: Dény
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D006508:Hepatitis Antibodies / Q000097:blood*; D003699:Hepatitis D / Q000276:immunology* / Q000821:virology; D003698:Hepatitis Delta Virus / Q000235:genetics* / Q000276:immunology*; D035923:Hepatitis delta Antigens / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000379:methods*; D016415:Sequence Alignment; D020349:Surface Plasmon Resonance; D055815:Young Adult
chemical_list: D006508:Hepatitis Antibodies; D035923:Hepatitis delta Antigens
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