title: An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients.
abstract: Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL(-1) and a linear detection range (LDR) of 0-0.1 U mL(-1). Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125.
authors: Research Centre for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. rashidi@tbzmed.ac.ir yomidi@tbzmed.ac.ir.
authors: M
authors: M
authors: Johari-Ahar
authors: M R
authors: MR
authors: Rashidi
authors: J
authors: J
authors: Barar
authors: M
authors: M
authors: Aghaie
authors: D
authors: D
authors: Mohammadnejad
authors: A
authors: A
authors: Ramazani
authors: P
authors: P
authors: Karami
authors: G
authors: G
authors: Coukos
authors: Y
authors: Y
authors: Omidi
mesh_terms: D000588:Amines / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000097:blood*; D019187:Cadmium Compounds; D058266:Dielectric Spectroscopy; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008565:Membrane Proteins / Q000097:blood*; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D010051:Ovarian Neoplasms / Q000097:blood*; D011422:Propionates / Q000737:chemistry; D018036:Selenium Compounds; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000588:Amines; D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D019187:Cadmium Compounds; C451759:MUC16 protein, human; D008565:Membrane Proteins; D011422:Propionates; D018036:Selenium Compounds; D006046:Gold; D012822:Silicon Dioxide; C058667:cadmium selenide