title: High pH microbial ecosystems in a newly discovered, ephemeral, serpentinizing fluid seep at Yanartaş (Chimera), Turkey.
abstract: Gas seeps emanating from Yanartaş (Chimera), Turkey, have been documented for thousands of years. Active serpentinization produces hydrogen and a range of carbon gases that may provide fuel for life. Here we report a newly discovered, ephemeral fluid seep emanating from a small gas vent at Yanartaş. Fluids and biofilms were sampled at the source and points downstream. We describe site conditions, and provide microbiological data in the form of enrichment cultures, Scanning electron microscopy (SEM), carbon and nitrogen isotopic composition of solids, and PCR screens of nitrogen cycle genes. Source fluids are pH 11.95, with a Ca:Mg of ~200, and sediments under the ignited gas seep measure 60°C. Collectively, these data suggest the fluid is the product of active serpentinization at depth. Source sediments are primarily calcite and alteration products (chlorite and montmorillonite). Downstream, biofilms are mixed with montmorillonite. SEM shows biofilms distributed homogeneously with carbonates. Organic carbon accounts for 60% of the total carbon at the source, decreasing downstream to <15% as inorganic carbon precipitates. δ(13)C ratios of the organic carbon fraction of solids are depleted (-25 to -28‰) relative to the carbonates (-11 to -20‰). We conclude that heterotrophic processes are dominant throughout the surface ecosystem, and carbon fixation may be key down channel. δ(15)N ratios ~3‰, and absence of nifH in extracted DNA suggest that nitrogen fixation is not occurring in sediments. However, the presence of narG and nirS at most locations and in enrichments indicates genomic potential for nitrate and nitrite reduction. This small seep with shallow run-off is likely ephemeral, but abundant preserved microterracettes in the outflow and the surrounding area suggest it has been present for some time. This site and others like it present an opportunity for investigations of preserved deep biosphere signatures, and subsurface-surface interactions.
authors: Department of Earth and Environmental Sciences, University of Illinois at Chicago Chicago, IL, USA.
authors: D'Arcy R
authors: DR
authors: Meyer-Dombard
authors: Department of Earth and Environmental Sciences, University of Illinois at Chicago Chicago, IL, USA.
authors: Kristin M
authors: KM
authors: Woycheese
authors: Department of Earth and Environmental Sciences, University of Illinois at Chicago Chicago, IL, USA.
authors: Erin N
authors: EN
authors: Yargıçoğlu
authors: Department of Geosciences, University of Rhode Island Kingston, RI, USA.
authors: Dawn
authors: D
authors: Cardace
authors: School of Earth and Space Exploration, Arizona State University Tempe, AZ, USA ; Department of Chemistry and Biochemistry, Arizona State University Tempe, AZ, USA.
authors: Everett L
authors: EL
authors: Shock
authors: Faculty of Science, University of Istanbul Istanbul, Turkey.
authors: Yasemin
authors: Y
authors: Güleçal-Pektas
authors: Department of Freshwater Biology, Istanbul University Istanbul, Turkey.
authors: Mustafa
authors: M
authors: Temel
keywords: Tekirova ophiolite; Yanartaş (Chimera) Turkey; deep subsurface; high pH springs; serpentinization; ultramafic
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