title: Role of HLA-G1 in trophoblast cell proliferation, adhesion and invasion.
abstract: Trophoblast cells are important in embryo implantation and fetomaternal tolerance. HLA-G is specifically expressed at the maternal-fetal interface and is a regulator in pregnancy. The aim of the present study was to detect the effect of HLA-G1 on trophoblast cell proliferation, adhesion, and invasion. Human trophoblast cell lines (JAR and HTR-8/SVneo cells) were infected with HLA-G1-expressing lentivirus. After infection, HLA-G1 expression of the cells was detected by western blotting. Cell proliferation was detected by the BrdU assay. The cell cycle and apoptosis of JAR and HTR-8/SVneo cells was measured by flow cytometry (FCM). The invasion of the cells under different conditions was detected by the transwell invasion chamber assay. HLA-G1 didn't show any significant influence on the proliferation, apoptosis, adhesion, and invasion of trophocytes in normal culture conditions. However, HLA-G1 inhibited JAR and HTR-8/SVneo cells invasion induced by hepatocyte growth factor (HGF) under normal oxygen conditions. In conditions of hypoxia, HLA-G1 couldn't inhibit the induction of cell invasion by HGF. HLA-G1 is not an independent factor for regulating the trophocytes. It may play an indirect role in embryo implantation and formation of the placenta.
authors: Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi'an 710038, China. Electronic address: jiangfeng1161@163.com.
authors: Feng
authors: F
authors: Jiang
authors: Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi'an 710038, China.
authors: Hongxi
authors: H
authors: Zhao
authors: Department of Gynecology and Obstetrics, The Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.
authors: Li
authors: L
authors: Wang
authors: Assisted Reproductive Center, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
authors: Xinyu
authors: X
authors: Guo
authors: Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi'an 710038, China.
authors: Xiaohong
authors: X
authors: Wang
authors: Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi'an 710038, China.
authors: Guowu
authors: G
authors: Yin
authors: Department of Orthopedics, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
authors: Yunsheng
authors: Y
authors: Hu
authors: Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi'an 710038, China.
authors: Yi
authors: Y
authors: Li
authors: Department of Gynecology and Obstetrics, The Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China. Electronic address: yuanqingyaoxa@163.com.
authors: Yuanqing
authors: Y
authors: Yao
mesh_terms: D017209:Apoptosis; D002448:Cell Adhesion; D002453:Cell Cycle / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D059951:HLA-G Antigens / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism / Q000473:pathology
chemical_list: D059951:HLA-G Antigens; D017228:Hepatocyte Growth Factor
keywords: Adhesion; Embryo implantation; HLA-G1; Invasion; Proliferation; Trophoblast cells