title: STAT3 regulation the expression of VEGF-D in HGC-27 gastric cancer cell.
abstract: OBJECTIVE
  To explore the potential mechanism of vascular endothelial growth factor D (VEGF-D) contribution to the lymphangiogenesis was regulated by the signal transducer and activator of transcription 3 (STAT3).
  
  
  METHODS
  We detected the expression in GC tissue, adjacent non-tumor tissue, GC cell lines (AGS, SUN-1, KATO-III, BGC-823, MGC-803, SGC-7901, and HGC-27), and GES-1 cell line. STAT3 siRNA transfection and genome microarray were applied to demonstrate whether the expression of VEGF-D was mediated by the STAT3 in GC.
  
  
  RESULTS
  We showed the STAT3, pSTAT3, and VEGF-D expression in GC tissue was significantly higher than those in adjacent non-tumor tissue, respectively. In addition, both STAT3 and VEGF-D mRNA expression was much higher in each GC cell line than those in GES-1 cell line. With STAT3 siRNA transfection, we demonstrated that VEGF-D expression level decreased significantly in HGC-27 cell by using the genome microarray representing STAT3 potential regulation the VEGF-D expression.
  
  
  CONCLUSIONS
  STAT3, a novel signal transducer inactivating in the GC cell, can contribute to the lymph node metastasis by promoting lymphangiogenesis via up-regulation expression of VEGF-D.
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Jingyu
authors: J
authors: Deng
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Jingli
authors: J
authors: Cui
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Nan
authors: N
authors: Jiang
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Rupeng
authors: R
authors: Zhang
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Li
authors: L
authors: Zhang
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Xishan
authors: X
authors: Hao
authors: Department of Gastroenterology, Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy, Nation Clinical Center of Cancer Tianjin, China.
authors: Han
authors: H
authors: Liang
keywords: Stomach; lymphatic metastasis; neoplasm; signal transducer and activator of transcription 3; vascular endothelial growth factor
references: Tumour Biol. 2013 Dec;34(6):3627-36
references: 23824571
references: Cancer Sci. 2004 Apr;95(4):328-33
references: 15072591
references: EMBO J. 2001 Feb 15;20(4):672-82
references: 11179212
references: Mol Med Rep. 2012 Jun;5(6):1438-42
references: 22469989
references: J Pathol. 2003 Dec;201(4):544-54
references: 14648657
references: Exp Ther Med. 2012 Oct;4(4):633-639
references: 23170117
references: J Gastrointest Surg. 2011 Sep;15(9):1526-31
references: 21717282
references: Int J Clin Oncol. 2005 Oct;10(5):318-27
references: 16247658
references: Pathol Int. 2007 Nov;57(11):712-8
references: 17922682
references: Neoplasma. 2012;59(1):52-61
references: 22082308
references: Curr Mol Med. 2009 Jun;9(5):626-33
references: 19601811
references: World J Surg. 2011 May;35(5):1010-6
references: 21387130
references: J Surg Res. 2011 Jun 15;168(2):188-96
references: 20189585
references: J Clin Pathol. 2006 Jan;59(1):77-82
references: 16394285
references: Oncol Rep. 2010 Oct;24(4):1093-9
references: 20811694
references: Asian Pac J Cancer Prev. 2012;13(6):2873-7
references: 22938476
references: Oncol Rep. 2008 Jan;19(1):33-9
references: 18097573
references: J Breast Cancer. 2013 Mar;16(1):40-9
references: 23593080
references: Int J Clin Exp Pathol. 2011 Apr;4(4):356-62
references: 21577321
references: Asian Pac J Cancer Prev. 2012;13(7):3089-97
references: 22994715
references: Hepatogastroenterology. 2013 Mar-Apr;60(122):277-80
references: 23574654
references: J Korean Med Sci. 2008 Aug;23(4):592-7
references: 18756043
references: J Pathol. 2009 Jun;218(2):192-200
references: 19224540
references: World J Gastroenterol. 2010 Nov 14;16(42):5380-7
references: 21072904
references: Tumour Biol. 2013 Jun;34(3):1493-501
references: 23475632