title: Eudoraea chungangensis sp. nov., isolated from an aquafarm waste water sludge.
abstract: A Gram-stain negative, non-spore-forming, non-motile, strictly aerobic bacterial strain, designated CAU 1296(T), was isolated from an aquafarm waste water sludge and its taxonomic position was investigated using a polyphasic approach. Cells of strain CAU 1296(T) grew optimally at 30 °C, at pH 7.5 and in the presence of 4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1296(T) formed a distinct lineage within the genus Eudoraea and exhibited similarity to Eudoraea adriatica AS06/20a(T) (95.4 % similarity). The major cellular fatty acids of the isolate were iso-C15:0, iso-C15:1 G, and summed feature 3 (C16:1 ω6c and C16:1 ω7c). The polar lipid pattern of strain CAU 1296(T) consisted of phosphatidylethanolamine, and unidentified lipids including a phosphoglycolipid, phospholipid, glycolipid, aminophospholipid, two aminolipids, and six further unidentified lipids. The strain contained menaquinone-6 (MK-6) as the major isoprenoid quinone. The G+C content of the genomic DNA was 38.7 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain CAU 1296(T) should be classified as a novel species in the genus Eudoraea, for which the name Eudoraea chungangensis sp. nov. is proposed. The type strain is CAU 1296(T) (=KCTC 42048(T), =CCUG 66239(T), =CECT 8744(T)).
authors: Department of Microbiology, College of Medicine, Chung-Ang University (CAU), Seoul, South Korea.
authors: Chatuphon
authors: C
authors: Siamphan
authors: Young-Hyo
authors: YH
authors: Chang
authors: Wonyong
authors: W
authors: Kim
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D007220:Industrial Waste; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012965:Sodium Chloride; D024482:Vitamin K 2; D062065:Wastewater