title: Trichoderma reesei Sch9 and Yak1 regulate vegetative growth, conidiation, and stress response and induced cellulase production.
abstract: Protein kinases are key players in controlling many basic cellular processes in almost all the organisms via mediating signal transduction processes. In the present study, we characterized the cellulolytic Trichoderma reesei orthologs of Saccharomyces cerevisiae Sch9 and Yak1 by sequence alignment and functional analysis. The T. reesei Trsch9Δ and Tryak1Δ mutant strains displayed a decreased growth rate on different carbon sources and produced less conidia. The absence of these two kinases also resulted in different but abnormal polarized apical growth as well as sensitivity to various stresses. In addition, disruption of the genes Trsch9 or Tryak1 resulted in perturbation of cell wall integrity. Interestingly, while the induced production of cellulases was slightly compromised in the Trsch9Δ strain, the extracellular production of cellulases was significantly improved in the absence of Yak1. The results indicate that TrSch9 and TrYak1 play an important role in filamentous growth, stress response and induced production of cellulases in T. reesei.
authors: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No.27 Shanda South Road, Jinan, 250100, Shandong, P. R. China.
authors: Xinxing
authors: X
authors: Lv
authors: Weixin
authors: W
authors: Zhang
authors: Guanjun
authors: G
authors: Chen
authors: Weifeng
authors: W
authors: Liu
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D009154:Mutation; D025282:Mycelium / Q000302:isolation & purification / Q000648:ultrastructure; D010802:Phylogeny; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000254:growth & development*; D013312:Stress, Physiological; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C071569:SCH9 protein kinase; D017346:Protein Serine-Threonine Kinases; C530964:SCH9 protein, S cerevisiae; C062391:YAK1 protein, S cerevisiae; D002480:Cellulase
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