title: Chemical synthesis of dinucleotide cap analogs.
abstract: This unit describes a reliable, efficient and general method for the synthesis of standard cap analog (mCAP), m(7)G[5']ppp[5']G, and anti-reverse cap analog (ARCA), m(7,3')(O)G[5']ppp[5']G. The synthesis of required intermediate m(7)GDP or m(2)(7,3'O)GDP has been achieved through regioselective methylation of the corresponding diphosphate using dimethyl sulfate under aqueous conditions. Then, the coupling reaction of m(7)GDP or m(2)(7,3'O)GDP with ImGMP using ZnCl(2)/DMF system affords the corresponding cap analog in good yields.
authors: Bioorganic Chemistry Division, Life Technologies Corporation, Austin, Texas.
authors: Anilkumar R
authors: AR
authors: Kore
authors: Muthian
authors: M
authors: Shanmugasundaram
mesh_terms: D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D012314:RNA Cap Analogs
keywords: anti–reverse cap analog; cap analog; eukaryotic mRNA; mRNA translation; regioselective methylation