title: Mucus Barriers to Microparticles and Microbes are Altered in Hirschsprung's Disease.
abstract: Mucus forms a protective hydrogel layer over the intestinal epithelium, presenting a selective and robust barrier to the uptake of particulates and microbe invasion. Disease can alter mucus production and composition, thus potentially modifying mucosal barrier properties. Hirschsprung's disease (HD) is a developmental abnormality of the nervous system often complicated by intestinal infection. An investigation of colonic mucus barrier properties in an HD animal model, endothelin receptor B mutant mice, revealed significantly reduced microsphere (passive) and microbe (active) transport rates (7-fold and 3.6-fold, respectively, in proximal colonic mucus) relative to wild-type. Transport differences were evident in both the ganglionic and aganglionic colon segments, in agreement with the risk of HD-associated enterocolitis after surgery to remove aganglionic colon segments. The development of therapies aimed at altering colonic mucus barrier properties could be explored towards preventing the onset of enterocolitis in HD.
authors: Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, 02115, USA.
authors: Hasan M
authors: HM
authors: Yildiz
authors: Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, 02115, USA.
authors: Taylor L
authors: TL
authors: Carlson
authors: Department of Pediatric Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA.
authors: Allan M
authors: AM
authors: Goldstein
authors: Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, 02115, USA. rebecca@coe.neu.edu.
authors: Rebecca L
authors: RL
authors: Carrier
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D003106:Colon / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000502:physiology*; D006627:Hirschsprung Disease / Q000382:microbiology* / Q000473:pathology*; D051379:Mice; D009093:Mucus / Q000382:microbiology*; D044023:Receptor, Endothelin B / Q000172:deficiency / Q000378:metabolism
chemical_list: C000602969:EDNRB protein, mouse; D044023:Receptor, Endothelin B
keywords: Hirschsprung's disease; intestine; microbe transport; mucus; nanoparticle diffusion
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