title: Effects of some natural carotenoids on TRPA1- and TRPV1-induced neurogenic inflammatory processes in vivo in the mouse skin.
abstract: Mechanisms of the potent anti-inflammatory actions of carotenoids are unknown. Since carotenoids are incorporated into membranes, they might modulate transient receptor potential ankyrin 1 and vanilloid 1 (TRPA1 and TRPV1) activation predominantly on peptidergic sensory nerves. We therefore investigated the effects of three carotenoids (β-carotene, lutein and lycopene) on cutaneous neurogenic inflammation. Acute neurogenic edema and inflammatory cell recruitment were induced by smearing the TRPA1 agonist mustard oil (5%) or the TRPV1 activator capsaicin (2.5%) on the mouse ear. Ear thickness was then determined by micrometry, microcirculation by laser Doppler imaging and neutrophil accumulation by histopathology and spectrophotometric determination of myeloperoxidase activity. The effects of lutein on the stimulatory action of the TRPA1 agonist mustard oil were also tested on the guinea-pig small intestine, in isolated organ experiments. Mustard oil evoked 50-55% ear edema and granulocyte influx, as shown by histology and myeloperoxidase activity. Swelling was significantly reduced between 2 and 4 h after administration of lutein or β-carotene (100 mg/kg subcutane three times during 24 h). Lutein also decreased neutrophil accumulation induced by TRPA1 activation, but did not affect mustard oil-evoked intestinal contraction. Lycopene had no effect on any of these parameters. None of the three carotenoids altered capsaicin-evoked inflammation. It is proposed that the dihydroxycarotenoid lutein selectively inhibits TRPA1 activation and consequent neurogenic inflammation, possibly by modulating lipid rafts.
authors: Department of Pharmacognosy, Medical School, University of Pécs, Rókus utca 2., Pécs, 7624, Hungary, gyorgyi.horvath@aok.pte.hu.
authors: Györgyi
authors: G
authors: Horváth
authors: Ágnes
authors: Á
authors: Kemény
authors: Loránd
authors: L
authors: Barthó
authors: Péter
authors: P
authors: Molnár
authors: József
authors: J
authors: Deli
authors: Lajos
authors: L
authors: Szente
authors: Tamás
authors: T
authors: Bozó
authors: Szilárd
authors: S
authors: Pál
authors: Katalin
authors: K
authors: Sándor
authors: Éva
authors: É
authors: Szőke
authors: János
authors: J
authors: Szolcsányi
authors: Zsuzsanna
authors: Z
authors: Helyes
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D002338:Carotenoids / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006168:Guinea Pigs; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009149:Mustard Plant; D020078:Neurogenic Inflammation / Q000188:drug therapy* / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels / Q000819:agonists / Q000378:metabolism*; D050051:Transient Receptor Potential Channels / Q000819:agonists / Q000378:metabolism*
chemical_list: D010938:Plant Oils; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D050051:Transient Receptor Potential Channels; C475685:Trpa1 protein, mouse; D002338:Carotenoids; D002211:Capsaicin; C027793:mustard oil
references: Neuropeptides. 2004 Dec;38(6):377-84
references: 15567473
references: Basic Clin Pharmacol Toxicol. 2013 Aug;113(2):141-3
references: 23438208
references: Neuroscience. 2004;125(2):449-59
references: 15062987
references: Curr Opin Allergy Clin Immunol. 2007 Oct;7(5):365-73
references: 17873574
references: Ann N Y Acad Sci. 1998 Nov 20;854:443-7
references: 9928451
references: Eur J Pharmacol. 2012 Aug 15;689(1-3):56-64
references: 22721614
references: Eur J Pharmacol. 2010 Feb 25;628(1-3):67-74
references: 19958765
references: Clin Nutr. 2003 Oct;22(5):459-62
references: 14512033
references: J Mol Neurosci. 2012 Jan;46(1):1-9
references: 21541689
references: BJU Int. 2008 Mar;101 Suppl 3:2-6
references: 18307678
references: Biochim Biophys Acta. 2007 Jan;1768(1):167-74
references: 17070769
references: Eur J Pharmacol. 2006 Mar 8;533(1-3):207-14
references: 16464449
references: Eur J Pharmacol. 2004 Oct 1;500(1-3):143-57
references: 15464028
references: Nat Rev Mol Cell Biol. 2000 Oct;1(1):31-9
references: 11413487
references: Arch Biochem Biophys. 2009 Mar 15;483(2):165-8
references: 18848517
references: Am J Cardiol. 2008 May 22;101(10A):20D-29D
references: 18474269
references: Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;305(1):75-81
references: 31571
references: Am J Cardiol. 2008 May 22;101(10A):4D-13D
references: 18474273
references: Acta Physiol (Oxf). 2007 May;190(1):47-53
references: 17428232
references: Mol Aspects Med. 2005 Dec;26(6):459-516
references: 16309738
references: Biomed Pharmacother. 2004 Mar;58(2):100-10
references: 14992791
references: Neuroscience. 1999 Apr;90(1):221-8
references: 10188948
references: Am J Cardiol. 2008 May 22;101(10A):58D-68D
references: 18474276