title: Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand.
abstract: Two Gram-stain-positive, endospore-forming lactic acid bacteria, designated BK92(T) and BK117-1(T), were isolated from tree barks in Thailand. Cells were catalase-negative and facultatively anaerobic rods. 16S rRNA gene sequence analysis indicated that these strains belonged to the genus Sporolactobacillus . Strains BK92(T) and BK117-1(T) showed the highest 16S rRNA gene sequence similarity to Sporolactobacillus putidus QC81-06(T) with 97.7% and 97.1% similarity, respectively. Analysis of phylogenetic relationships based on 16S rRNA and gyrB gene sequencing revealed that the positions of strains BK92(T) and BK117-1(T) were clearly separated from all related species of the genus Sporolactobacillus . Strains BK92(T) and BK117-1(T) had low DNA-DNA relatedness between each other and also with S. putidus QC81-06(T) and Sporolactobacillus vineae SL153(T). The DNA G+C content of strains BK92(T) and BK117-1(T) was 46.6 mol% and 47.4 mol%, respectively. The major fatty acids of strains BK92(T) and BK117-1(T) were anteiso-C(17 : 0) and anteiso-C(15 : 0). They contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant menaquinone. Based on evidence including phenotypic, genotypic and chemotaxonomic studies, strains BK92(T) and BK117-1(T) should be classified as representatives of novel species of the genus Sporolactobacillus , for which the names Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov. are proposed, respectively. The type strains are BK92(T) ( = JCM 19541(T) = LMG 28365(T) = PCU 336(T) = TISTR 2234(T)) and BK117-1(T) ( = JCM 19542(T) = LMG 28366(T) = PCU 337(T) = TISTR 2235(T)).
authors: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Tanatip
authors: T
authors: Thamacharoensuk
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors: Maki
authors: M
authors: Kitahara
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors: Moriya
authors: M
authors: Ohkuma
authors: Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Nuttha
authors: N
authors: Thongchul
authors: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Somboon
authors: S
authors: Tanasupawat
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D019344:Lactic Acid; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial; D013785:Thailand; D014197:Trees / Q000382:microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D019344:Lactic Acid; D003960:Diaminopimelic Acid; C062629:menaquinone 7