title: Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst.
abstract: The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12- ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors: Byeong-Hun
authors: BH
authors: Lee
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors: Sae-Bom
authors: SB
authors: Lee
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors: Hyeon-Soo
authors: HS
authors: Kim
authors: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors: Ki-Jun
authors: KJ
authors: Jeong
authors: Department of Food Science and Engineering, Ewha Woman's University, Seoul 120-750, Republic of Korea.
authors: Jinb-Yung
authors: JY
authors: Park
authors: Department of Biological and Chemical Engineering, Hongik University, Sejong 339-701, Republic of Korea.
authors: Kyung-Moon
authors: KM
authors: Park
authors: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors: Jin-Won
authors: JW
authors: Lee
mesh_terms: D055162:Biocatalysis / Q000187:drug effects; D019149:Bioreactors; D001711:Biotransformation / Q000187:drug effects; D002021:Buffers; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D005285:Fermentation; D005818:Genetic Engineering; D006863:Hydrogen-Ion Concentration; D009829:Oleic Acids / Q000378:metabolism; D012277:Ricinoleic Acids / Q000378:metabolism*; D013696:Temperature
chemical_list: D002021:Buffers; D003902:Detergents; D009829:Oleic Acids; D012277:Ricinoleic Acids; C048050:12-ketooleic acid; C030521:ricinoleic acid