title: Coupling Freshly Isolated CD44(+) Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis.
abstract: An alternative strategy to the use of in vitro expanded cells in regenerative medicine is the use of freshly isolated stromal cells, where a bioactive scaffold is used to provide an environment conducive to proliferation and tissue-specific differentiation in vivo. The objective of this study is to develop a cartilage extracellular matrix (ECM)-derived scaffold that could facilitate the rapid proliferation and chondrogenic differentiation of freshly isolated stromal cells. By freeze-drying cryomilled cartilage ECM of differing concentrations, it is possible to produce scaffolds with a range of pore sizes. The migration, proliferation, and chondrogenic differentiation of infrapatellar fat pad-derived stem cells (FPSCs) depend on the concentration/porosity of these scaffolds, with greater sulphated glycosaminoglycan (sGAG) accumulation observed in scaffolds with larger-sized pores. It is then sought to determine if freshly isolated fat pad-derived stromal cells, seeded onto a transforming growth factor (TGF)-β3 eluting ECM-derived scaffold, could promote chondrogenesis in vivo. While a more cartilage-like tissue could be generated using culture expanded FPSCs compared to nonenriched freshly isolated cells, fresh CD44(+) stromal cells are capable of producing a tissue in vivo that stained strongly for sGAGs and type II collagen. These findings open up new possibilities for in-theatre cell-based therapies for joint regeneration.
authors: Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.|Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.
authors: Henrique V
authors: HV
authors: Almeida
authors: Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.|Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.
authors: Gráinne M
authors: GM
authors: Cunniffe
authors: School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors: Tatiana
authors: T
authors: Vinardell
authors: Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.|Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.
authors: Conor T
authors: CT
authors: Buckley
authors: Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.|Department of Anatomy, Royal College of Surgeons in Ireland, Dublin 2, Ireland.|Advanced Materials and Bioengineering Research Centre (AMBER), Trinity College Dublin and RCSI, Dublin 2, Ireland.
authors: Fergal J
authors: FJ
authors: O'Brien
authors: Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.|Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.|Department of Anatomy, Royal College of Surgeons in Ireland, Dublin 2, Ireland.|Advanced Materials and Bioengineering Research Centre (AMBER), Trinity College Dublin and RCSI, Dublin 2, Ireland.
authors: Daniel J
authors: DJ
authors: Kelly
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D008875:Middle Aged; D012038:Regeneration / Q000502:physiology; D044968:Regenerative Medicine / Q000379:methods; D017154:Stromal Cells / Q000378:metabolism*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*
chemical_list: D024043:Collagen Type II; D018960:Hyaluronan Receptors; D053782:Transforming Growth Factor beta3
keywords: articular cartilage; extracellular matrix; scaffolds; single-stage therapies