title: Establishing a China malaria diagnosis reference laboratory network for malaria elimination.
abstract: BACKGROUND
  In China, the prevalence of malaria has reduced dramatically due to the elimination programme. The continued success of the programme will depend upon the accurate diagnosis of the disease in the laboratory. The basic requirements for this are a reliable malaria diagnosis laboratory network and quality management system to support case verification and source tracking.
  
  
  METHODS
  The baseline information of provincial malaria laboratories in the China malaria diagnosis reference laboratory network was collected and analysed, and a quality-assurance activity was carried out to assess their accuracies in malaria diagnosis by microscopy using WHO standards and PCR.
  
  
  RESULTS
  By the end of 2013, nineteen of 24 provincial laboratories have been included in the network. In the study, a total of 168 staff were registered and there was no bias in their age, gender, education level, and position. Generally Plasmodium species were identified with great accuracy by microscopy and PCR. However, Plasmodium ovale was likely to be misdiagnosed as Plasmodium vivax by microscopy.
  
  
  CONCLUSIONS
  China has established a laboratory network for primary malaria diagnosis which will cover a larger area. Currently, Plasmodium species can be identified fairly accurately by microscopy and PCR. However, laboratory staff need additional trainings on accurate identification of P. ovale microscopically and good performance of PCR operations.
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. chart2543@163.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. chart2543@163.com.
authors: Jian-hai
authors: JH
authors: Yin
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. yanhe1012@126.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. yanhe1012@126.com.
authors: He
authors: H
authors: Yan
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. ipdhuangfang@163.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. ipdhuangfang@163.com.
authors: Fang
authors: F
authors: Huang
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. li_mei76@163.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. li_mei76@163.com.
authors: Mei
authors: M
authors: Li
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. huihuixiao1001@126.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. huihuixiao1001@126.com.
authors: Hui-hui
authors: HH
authors: Xiao
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. zss163@hotmail.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. zss163@hotmail.com.
authors: Shui-sen
authors: SS
authors: Zhou
authors: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, Shanghai, People's Republic of China. nipdxzhg@163.com.|WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, People's Republic of China. nipdxzhg@163.com.
authors: Zhi-gui
authors: ZG
authors: Xia
mesh_terms: D002681:China / Q000453:epidemiology; D019411:Clinical Laboratory Techniques / Q000379:methods / Q000592:standards; D003955:Diagnostic Tests, Routine / Q000379:methods / Q000592:standards; D060740:Disease Eradication / Q000379:methods*; D006801:Humans; D007753:Laboratories / Q000458:organization & administration*; D008288:Malaria / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control*; D008853:Microscopy / Q000379:methods / Q000592:standards; D010961:Plasmodium / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards
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