title: Influence of xanthan gum on the structural characteristics of myofibrillar proteins treated by high pressure.
abstract: The effects of xanthan gum on the structural modifications of myofibrillar proteins (0.3 M NaCl, pH 6) induced by high pressure (200, 400, and 600 MPa, 6 min) were investigated. The changes in the secondary and tertiary structures of myofibrillar proteins were analyzed by circular dichroism. The protein denaturation was also evaluated by differential scanning calorimetry. Likewise, the protein surface hydrophobicity and the solubility of myofibrillar proteins were measured. High pressure (600 MPa) induced the loss of α-helix structures and an increase of β-sheet structures. However, the presence of xanthan gum hindered the former mechanism of protein denaturation by high pressure. In fact, changes in the secondary (600 MPa) and the tertiary structure fingerprint of high-pressure-treated myofibrillar proteins (400 to 600 MPa) were observed in the presence of xanthan gum. These modifications were confirmed by the thermal analysis, the thermal transitions of high-pressure (400 to 600 MPa)-treated myofibrillar proteins were modified in systems containing xanthan gum. As consequence, the high-pressure-treated myofibrillar proteins with xanthan gum showed increased solubility from 400 MPa, in contrast to high-pressure treatment (600 MPa) without xanthan gum. Moreover, the surface hydrophobicity of high-pressure-treated myofibrillar proteins was enhanced in the presence of xanthan gum. These effects could be due to the unfolding of myofibrillar proteins at high-pressure levels, which exposed sites that most likely interacted with the anionic polysaccharide. This study suggests that the role of food additives could be considered for the development of meat products produced by high-pressure processing.
authors: ONIRIS, Food Process Engineering, UMR CNRS 6144 GEPEA, CS 82225, 44322, Nantes, Cedex 3, France.
authors: Gina
authors: G
authors: Villamonte
authors: Vanessa
authors: V
authors: Jury
authors: Stéphanie
authors: S
authors: Jung
authors: Marie
authors: M
authors: de Lamballerie
mesh_terms: D002152:Calorimetry, Differential Scanning; D005503:Food Additives / Q000737:chemistry*; D008461:Meat Products / Q000032:analysis*; D009124:Muscle Proteins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011312:Pressure; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012995:Solubility
chemical_list: D005503:Food Additives; D009124:Muscle Proteins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C002563:xanthan gum
keywords: High-pressure processing; myofibrillar protein; protein structure; xanthan gum