title: Effect-directed analysis and mixture effects of AhR-active PAHs in crude oil and coastal sediments contaminated by the Hebei Spirit oil spill.
abstract: The major AhR-active PAHs were identified in crude oil and oil-contaminated sediments by use of effect-directed analysis. As part of the study, an enhanced potency balance analysis was conducted by establishing the novel relative potency values of (alkyl)-PAHs from the H4IIE-luc bioassay. Silica gel column fractionation of crude oil resulted in greater AhR-mediated potencies in fractions of aromatics (F2) and resins (F3), and such trend was also observed for field collected sediment samples. AhR-mediated potencies of six F2 sub-fractions from HPLC indicated that the majority of F2 responses were attributable to 3-4 ring aromatics. Target PAHs including C4-phenanthrene, C1-chrysene, and C3-chrysene in sediments explained ∼ 18% of the bioassay-derived TCDD-EQs, however, the unknown AhR agonists and potential mixture effects remain in question. Overall, the AhR-potency and antagonistic potential of residual oil in sediment tended to decrease over time, thus monitoring of weathering process would be key for the post management of oil-contaminated sites.
authors: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea; Division of Environmental Sciences and Ecological Engineering, Korea University, Seoul, Republic of Korea.
authors: Seongjin
authors: S
authors: Hong
authors: Department of Environmental Health, Seoul National University, Seoul, Republic of Korea.
authors: Sangwoo
authors: S
authors: Lee
authors: Department of Environmental Health, Seoul National University, Seoul, Republic of Korea.
authors: Kyungho
authors: K
authors: Choi
authors: Institute of Marine Industry, Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea.
authors: Gi Beum
authors: GB
authors: Kim
authors: Oil and POPs Research Group, Korea Institute of Ocean Science and Technology (KIOST), Geoje, Republic of Korea.
authors: Sung Yong
authors: SY
authors: Ha
authors: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
authors: Bong-Oh
authors: BO
authors: Kwon
authors: Department of Marine Biotechnology, Anyang University, Ganghwa-gun, Incheon, Republic of Korea.
authors: Jongseong
authors: J
authors: Ryu
authors: Oil and POPs Research Group, Korea Institute of Ocean Science and Technology (KIOST), Geoje, Republic of Korea.
authors: Un Hyuk
authors: UH
authors: Yim
authors: Oil and POPs Research Group, Korea Institute of Ocean Science and Technology (KIOST), Geoje, Republic of Korea.
authors: Won Joon
authors: WJ
authors: Shim
authors: Division of Environmental Sciences and Ecological Engineering, Korea University, Seoul, Republic of Korea.
authors: Jinho
authors: J
authors: Jung
authors: Department of Veterinary Biomedical Sciences & Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Department of Zoology & Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Biology and Chemistry & State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, SAR, China.
authors: John P
authors: JP
authors: Giesy
authors: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea. Electronic address: jskocean@snu.ac.kr.
authors: Jong Seong
authors: JS
authors: Khim
mesh_terms: D001681:Biological Assay / Q000379:methods; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000032:analysis / Q000737:chemistry*; D010578:Petroleum / Q000032:analysis* / Q000633:toxicity; D059392:Petroleum Pollution; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity
chemical_list: D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical
keywords: Effect-directed analysis; H4IIE-luc bioassay; Oil weathering; Polycyclic aromatic hydrocarbons; Sediments; Yellow Sea