title: Correlation of circulating betatrophin concentrations with insulin secretion capacity, evaluated by glucagon stimulation tests.
abstract: OBJECTIVE
  To investigate the relationship between plasma betatrophin concentrations and insulin secretion capacity in people with Type 2 diabetes.
  
  
  METHODS
  Glucagon stimulation tests (1 mg) were performed in 70 people with Type 2 diabetes after an overnight fast. Plasma betatrophin concentrations were measured using an enzyme-linked immunosorbent assay. Insulin secretion capacity was evaluated by measuring increments of C-peptide concentration in response to glucagon stimulation, and creatinine clearance was determined by comparing creatinine concentrations in serum and 24-h urine samples.
  
  
  RESULTS
  Plasma betatrophin concentrations were positively correlated with duration of Type 2 diabetes (r = 0.34, P = 0.003), and negatively correlated with increments of C-peptide concentration (r = 0.37, P = 0.001) and creatinine clearance (r = 0.37, P = 0.001). The correlation with increments of C-peptide concentration remained significant after adjustment for age and duration of Type 2 diabetes (r = 0.25, P = 0.037). Multivariate analysis identified age and increments of C-peptide concentration as independent factors associated with plasma betatrophin levels.
  
  
  CONCLUSIONS
  Plasma betatrophin levels inversely correlate with insulin secretion capacity, suggesting that betatrophin levels are regulated by insulin secretion capacity in humans.
authors: Centre for Diabetes and Endocrinology, Tazuke Kofukai Foundation, Medical Research Institute, Kitano Hospital, Osaka, Japan.
authors: S
authors: S
authors: Tokumoto
authors: Y
authors: Y
authors: Hamamoto
authors: K
authors: K
authors: Fujimoto
authors: E
authors: E
authors: Yamaguchi
authors: E
authors: E
authors: Okamura
authors: S
authors: S
authors: Honjo
authors: H
authors: H
authors: Ikeda
authors: Y
authors: Y
authors: Wada
authors: A
authors: A
authors: Hamasaki
authors: H
authors: H
authors: Koshiyama
mesh_terms: D000368:Aged; D000091306:Angiopoietin-Like Protein 8; D000075802:Angiopoietin-like Proteins; D015415:Biomarkers / Q000097:blood; D002096:C-Peptide / Q000097:blood; D003404:Creatinine / Q000097:blood / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D005260:Female; D005934:Glucagon / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000097:blood*; D050417:Insulin-Secreting Cells / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D036361:Peptide Hormones / Q000097:blood*; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: C576683:ANGPTL8 protein, human; D000091306:Angiopoietin-Like Protein 8; D000075802:Angiopoietin-like Proteins; D015415:Biomarkers; D002096:C-Peptide; D007328:Insulin; D036361:Peptide Hormones; D005934:Glucagon; D003404:Creatinine
references: Diabetologia. 2014 Jun;57(6):1204-8
references: 24623100
references: Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E334-51
references: 22569073
references: J Am Geriatr Soc. 1985 Apr;33(4):278-85
references: 3989190
references: Cell. 2013 May 9;153(4):747-58
references: 23623304
references: Diabetologia. 2014 Jan;57(1):50-3
references: 24078058
references: Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19751-6
references: 23150577
references: Biochem Biophys Res Commun. 2012 Aug 10;424(4):786-92
references: 22809513
references: Diabetes Res Clin Pract. 2008 Dec;82(3):353-8
references: 18950889
references: Diabet Med. 2015 May;32(5):653-6
references: 25655786
references: Diabetes Care. 2004 Nov;27(11):2648-53
references: 15505000
references: Metabolism. 2004 Jul;53(7):831-5
references: 15254872
references: Int J Cancer. 2004 Nov 1;112(2):239-48
references: 15352036
references: J Clin Endocrinol Metab. 2014 Oct;99(10):E2004-9
references: 25050901
references: Diabetes. 2014 Apr;63(4):1283-8
references: 24353178
references: Diabetes Care. 2014 Oct;37(10):2718-22
references: 25024395