title: Characterization of conformational dynamics of bistable RNA by equilibrium and non-equilibrium NMR.
abstract: Unlike proteins, a given RNA sequence can adopt more than a single conformation. The two (or more) conformations are long-lived and have similar stabilities, but interconvert only slowly. Such bi- or multistability is often linked to the biological functions of the RNA. This unit describes how nuclear magnetic resonance (NMR) spectroscopy can be used to characterize the conformational dynamics of bistable RNAs.
authors: Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany; Institute of Organic Chemistry and Chemical Biology, Cluster of Excellence Macromolecular Complexes, Johann Wolfgang Goethe University, Frankfurt, Germany.
authors: Boris
authors: B
authors: Fürtig
authors: Anke
authors: A
authors: Reining
authors: Florian
authors: F
authors: Sochor
authors: Eva Marie
authors: EM
authors: Oberhauser
authors: Alexander
authors: A
authors: Heckel
authors: Harald
authors: H
authors: Schwalbe
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D059370:RNA Folding
chemical_list: D012313:RNA
keywords: RNA folding; bistable RNA; exchange spectroscopy; photo‐caged RNA; real‐time NMR