title: Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum.
abstract: 9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell apoptosis by euptox A. The apoptosis‑inducing effect of the euptox A on HeLa cells was examined by MTT assay. The underlying mechanism was analyzed by flow cytometry and quantitative PCR. Flow cytometry results suggested that euptox A effectively inhibited the proliferation of HeLa cells, arrested the cell cycle transition from S to G2/M phase, did not continue to complete the cell cycle activity (mainly from 4 times and mitosis), and induced cell proliferation. The RT-qPCR detection results showed that euptox A induced apoptosis by improving the gene expression level of apoptotic proteases such as caspase-10 in HeLa cells. Its mechanism of action was associated with the upregulation of apoptotic gene expression and arresting of the cell cycle.
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Fei
authors: F
authors: Liao
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Yanchun
authors: Y
authors: Hu
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Lei
authors: L
authors: Wu
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Hui
authors: H
authors: Tan
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Biao
authors: B
authors: Luo
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Yajun
authors: Y
authors: He
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Yan
authors: Y
authors: Qiao
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Quan
authors: Q
authors: Mo
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Ya
authors: Y
authors: Wang
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Zhicai
authors: Z
authors: Zuo
authors: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
authors: Junliang
authors: J
authors: Deng
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an 710069, P.R. China.
authors: Yahui
authors: Y
authors: Wei
mesh_terms: D036603:Ageratina / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: C098165:9-oxo-10,11-dehydroagerophorone; D010936:Plant Extracts; D012717:Sesquiterpenes