title: Variants in miRNA regulating cardiac growth are not a common cause of hypertrophic cardiomyopathy.
abstract: OBJECTIVE
  A substantial proportion of patients with hypertrophic cardiomyopathy (HCM) do not have causative mutations in the genes for heart sarcomere. The purpose of this study was to evaluate the association between microRNA (miRNA) sequence variants and HCM.
  
  
  METHODS
  We performed genetic testing on 56 HCM patients who had previously been found to be negative for mutations in the 4 major genes for sarcomeric proteins. The coding and adjacent regions (120-220 nt) of selected miRNAs were analyzed for the presence of sequence variants. The testing was based on PCR amplification of DNA-encoding miRNAs and subsequent denaturing capillary electrophoresis.
  
  
  RESULTS
  A total of 3 different variants were detected in the 11 selected miRNAs. These included polymorphisms rs45489294 in miRNA 208b, rs13136737 in miRNA 367 and rs9989532 in miRNA 1-2. In the patient group, the most frequent polymorphism was in miRNA 208b (10 times) followed by miRNA 367 (7 times). Both polymorphisms were found to occur with similar frequencies in the group of healthy controls. The remaining detected variant was not present in the control group, but was not connected with the HCM phenotype in the children of the probands.
  
  
  CONCLUSIONS
  Sequence variants in miRNAs of patients with HCM are not frequent and the contribution of these variants to the development of this disease was not demonstrated.
authors: Cardiocenter, Department of Cardiology, 3rd Faculty of Medicine, Charles University and University Hospital Kralovske Vinohrady, Prague, Czech Republic.
authors: Karol
authors: K
authors: Curila
authors: Lucie
authors: L
authors: Benesova
authors: Pavol
authors: P
authors: Tomasov
authors: Barbora
authors: B
authors: Belsanova
authors: Petr
authors: P
authors: Widimsky
authors: Marek
authors: M
authors: Minarik
authors: David
authors: D
authors: Zemanek
authors: Josef
authors: J
authors: Veselka
authors: Pavel
authors: P
authors: Gregor
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics*; D004247:DNA; D005260:Female; D005820:Genetic Testing; D014644:Genetic Variation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: C553207:MIRN1-2 microRNA, human; C550906:MIRN208 microRNA, human; C568081:MIRN367 microRNA, human; D035683:MicroRNAs; D004247:DNA