title: Simple micellar electrokinetic chromatography method for the determination of hydrogen sulfide in hen tissues.
abstract: A new method for the determination of hydrogen sulfide in hen tissues has been developed and validated. For estimation of hydrogen sulfide content, a sample (0.1 g) of hen tissue was treated according to the procedure consisted of some essential steps: simultaneous homogenization of a tissue and derivatization of hydrogen sulfide to its S-quinolinium derivative with 2-chloro-1-methylquinolinium tetrafluoroborate, separation of so-formed derivative by micellar electrokinetic chromatography with sweeping, and detection and quantitation with the use of UV detector set to measure analytical signals at 375 nm. Effective electrophoretic separation was achieved using fused silica capillary (effective length 41.5 cm, 75 μm id) and 0.05 mol/L, pH 8 phosphate buffer with the addition of 0.04 mol/L SDS and 26% ACN. The lower limit of quantification was 0.12 μmol hydrogen sulfide in 1 g of tissue. The calibration curve prepared in tissue homogenate for hydrogen sulfide showed linearity in the range from 0.15 to 2.0 μmol/g, with the coefficient of correlation 0.9978. The relative standard deviation of the points of the calibration curve varied from 8.3 to 3.2% RSD.
authors: Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, Poland.
authors: Paweł
authors: P
authors: Kubalczyk
authors: Kamila
authors: K
authors: Borowczyk
authors: Grażyna
authors: G
authors: Chwatko
authors: Rafał
authors: R
authors: Głowacki
mesh_terms: D000818:Animals; D002138:Calibration; D002645:Chickens; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006862:Hydrogen Sulfide / Q000032:analysis*; D011806:Quinolinium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: C000607170:2-chloro-1-methylquinolinium; D011806:Quinolinium Compounds; D006862:Hydrogen Sulfide
keywords: Derivatization; Hydrogen sulfide; MEKC; Sweeping; Tissue