title: Expression and functional analysis of the plant-specific histone deacetylase HDT701 in rice.
abstract: Reversible histone acetylation and deacetylation at the N-terminus of histone tails play a crucial role in regulating eukaryotic gene activity. Acetylation of core histones is associated with gene activation, whereas deacetylation of histone is often correlated with gene repression. The level of histone acetylation is antagonistically catalyzed by histone acetyltransferases citation(HATs) and histone deacetylases (HDACs). In this work, we examined the subcellular localization, expression pattern and function of HDT701, a member of the plant-specific HD2-type histone deacetylase in rice. HDT701 is localized at the subcellular level in the nucleus. Histochemical GUS-staining analysis revealed that HDT701 is constitutively expressed throughout the life cycle of rice. Overexpression of HDT701 in rice decreases ABA, salt and osmotic stress resistance during seed germination. Delayed seed germination of HDT701 overexpression lines is associated with decreased histone H4 acetylation and down-regulated expression of GA biosynthetic genes. Moreover, overexpression of HDT701 in rice enhances salt and osmotic stress resistance during the seedling stage. Taken together, our findings suggested that HDT701 may play an important role in regulating seed germination in response to abiotic stresses in rice.
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China ; University of Chinese Academy of Sciences, Chinese Academy of Sciences Beijing, China.
authors: Jinhui
authors: J
authors: Zhao
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Jianxia
authors: J
authors: Zhang
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Wei
authors: W
authors: Zhang
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Kunlin
authors: K
authors: Wu
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Feng
authors: F
authors: Zheng
authors: Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada London, ON, Canada.
authors: Lining
authors: L
authors: Tian
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Xuncheng
authors: X
authors: Liu
authors: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors: Jun
authors: J
authors: Duan
keywords: HDT701; abiotic stress; gene expression; histone deacetylase; seed germination
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