title: Real-time immuno-PCR: an approach for detection of trace amounts of transgenic proteins.
abstract: The research on manipulation of crop genomes for transgenic development is continuously increasing due to several benefits. The major concerns linked to the effect of transgenic crops are human health and environment sustainability. To monitor transgenic samples in the food chain, several highly sensitive and specific DNA-based and protein-based detection methods are being used. However, real- time immunio-PCR (RT-IPCR) assay would be able to provide a sensitive detection of trace amounts of transgenic proteins or allergens in the samples and help in monitoring these materials. In the present study, we developed a novel RT-IPCR method to monitor CrylAc transgenic protein in samples with an LOD of 100 pg/mL. The assay may also be useful in the evaluation of functional stability of transgenes inserted in the plant genome.
authors: Rajesh
authors: R
authors: Kumar
authors: Rajeshwar P
authors: RP
authors: Sinha
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D018556:Crops, Agricultural / Q000235:genetics; D004731:Endotoxins / Q000032:analysis* / Q000235:genetics*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019076:Transgenes
chemical_list: D055910:Antibodies, Immobilized; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis