title: McMYB10 regulates coloration via activating McF3'H and later structural genes in ever-red leaf crabapple.
abstract: The ever-red leaf trait, which is important for breeding ornamental and higher anthocyanin plants, rarely appears in Malus families, but little is known about the regulation of anthocyanin biosynthesis involved in the red leaves. In our study, HPLC analysis showed that the anthocyanin concentration in ever-red leaves, especially cyanidin, was significantly higher than that in evergreen leaves. The transcript level of McMYB10 was significantly correlated with anthocyanin synthesis between the 'Royalty' and evergreen leaf 'Flame' cultivars during leaf development. We also found the ever-red leaf colour cultivar 'Royalty' contained the known R6 : McMYB10 sequence, but was not in the evergreen leaf colour cultivar 'Flame', which have been reported in apple fruit. The distinction in promoter region maybe is the main reason why higher expression level of McMYB10 in red foliage crabapple cultivar. Furthermore, McMYB10 promoted anthocyanin biosynthesis in crabapple leaves and callus at low temperatures and during long-day treatments. Both heterologous expression in tobacco (Nicotiana tabacum) and Arabidopsis pap1 mutant, and homologous expression in crabapple and apple suggested that McMYB10 could promote anthocyanins synthesis and enhanced anthocyanin accumulation in plants. Interestingly, electrophoretic mobility shift assays, coupled with yeast one-hybrid analysis, revealed that McMYB10 positively regulates McF3'H via directly binding to AACCTAAC and TATCCAACC motifs in the promoter. To sum up, our results demonstrated that McMYB10 plays an important role in ever-red leaf coloration, by positively regulating McF3'H in crabapple. Therefore, our work provides new perspectives for ornamental fruit tree breeding.
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Ji
authors: J
authors: Tian
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Zhen
authors: Z
authors: Peng
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Jie
authors: J
authors: Zhang
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Tingting
authors: T
authors: Song
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Huihua
authors: H
authors: Wan
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Meiling
authors: M
authors: Zhang
authors: Beijing Key Laboratory for Agricultural Application and New Technique, Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
authors: Yuncong
authors: Y
authors: Yao
mesh_terms: D018506:Gene Expression Regulation, Plant; D027845:Malus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D000073718:Pancreatitis-Associated Proteins; D010858:Pigmentation / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000073718:Pancreatitis-Associated Proteins; D010940:Plant Proteins; C000612141:REG3A protein, human
keywords: Malus crabapple; McMYB10; cyanidin; ever-red leaf; flavonoid 3′-hydroxylase; transformation