title: A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8.
abstract: The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1 g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.
authors: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
authors: Svenja
authors: 0000-0003-3569-034X
authors: S
authors: Fengler
authors: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
authors: Ina
authors: 0000-0002-8770-4486
authors: I
authors: Spirer
authors: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
authors: Maren
authors: M
authors: Neef
authors: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
authors: Margret
authors: M
authors: Ecke
authors: Center for Bioinformatics, University of Tübingen, Sand 14, 72076 Tübingen, Germany.
authors: Kay
authors: 0000-0002-1283-7065
authors: K
authors: Nieselt
authors: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany.
authors: Rüdiger
authors: R
authors: Hampp
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013026:Space Flight; D018531:Spacecraft; D013696:Temperature; D046509:Tissue Culture Techniques / Q000379:methods*; D014893:Weightlessness
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