title: Light and life in Baltimore--and beyond.
abstract: Baltimore has been the home of numerous biophysical studies using light to probe cells. One such study, quantitative measurement of lateral diffusion of rhodopsin, set the standard for experiments in which recovery after photobleaching is used to measure lateral diffusion. Development of this method from specialized microscopes to commercial scanning confocal microscopes has led to widespread use of the technique to measure lateral diffusion of membrane proteins and lipids, and as well diffusion and binding interactions in cell organelles and cytoplasm. Perturbation of equilibrium distributions by photobleaching has also been developed into a robust method to image molecular proximity in terms of fluorescence resonance energy transfer between donor and acceptor fluorophores.
authors: Biology Department, Johns Hopkins University, Baltimore, Maryland. Electronic address: edidin@jhu.edu.
authors: Michael
authors: M
authors: Edidin
mesh_terms: D000818:Animals; D015142:Baltimore; D015415:Biomarkers / Q000378:metabolism; D001703:Biophysics / Q000266:history*; D003226:Congresses as Topic / Q000266:history* / Q000639:trends; D036681:Fluorescence Recovery After Photobleaching / Q000266:history; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D008027:Light; D051379:Mice; D008856:Microscopy, Fluorescence / Q000266:history
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes
references: J Biochem Biophys Methods. 1980 Jan-Feb;2(1):91-119
references: 6158533
references: Annu Rev Biophys Biophys Chem. 1989;18:271-308
references: 2660827
references: Annu Rev Biochem. 1985;54:43-71
references: 3896124
references: J Cell Biol. 1987 Aug;105(2):755-60
references: 3624308
references: J Immunol. 1979 Nov;123(5):2161-7
references: 385777
references: Biophys J. 2006 Aug 15;91(4):1169-91
references: 16679358
references: Nat New Biol. 1972 Mar 15;236(63):39-43
references: 4537062
references: Proc Natl Acad Sci U S A. 1976 Jul;73(7):2409-13
references: 1065895
references: Methods Mol Biol. 2015;1232:255-71
references: 25331140
references: Immunity. 1999 Aug;11(2):231-40
references: 10485658
references: J Cell Biol. 1991 Oct;115(1):245-55
references: 1717480
references: Biophys J. 2000 Oct;79(4):1761-70
references: 11023884
references: Biophys J. 1999 May;76(5):2843-51
references: 10233100
references: Pharm Res. 2014 Feb;31(2):255-70
references: 24019022
references: Cytometry A. 2013 Sep;83(9):780-93
references: 23813736
references: Annu Rev Biophys Biomol Struct. 1997;26:373-99
references: 9241424
references: Nature. 1974 Feb 15;247(5441):438-41
references: 4818543
references: J Cell Biol. 1998 Jul 13;142(1):69-84
references: 9660864
references: Trends Biochem Sci. 2011 Nov;36(11):604-15
references: 21917465
references: Science. 1996 Aug 9;273(5276):797-801
references: 8670420
references: Methods Cell Biol. 1989;30:271-306
references: 2648113
references: Biochim Biophys Acta. 1976 Apr 16;433(1):215-22
references: 177080
references: Science. 1976 Feb 6;191(4226):466-8
references: 1246629
references: J Theor Biol. 1973 Jul;40(1):11-7
references: 4723546
references: Z Wiss Mikrosk. 1967 Nov;68(3):129-42
references: 4882659
references: Biophys J. 1976 Sep;16(9):1055-69
references: 786399
references: Biophys J. 1996 Jun;70(6):2767-73
references: 8744314
references: Biochim Biophys Acta. 1976 Apr 16;433(1):209-14
references: 1260061
references: J Cell Biol. 1997 Sep 22;138(6):1193-206
references: 9298976
references: J Cell Sci. 1970 Sep;7(2):319-35
references: 4098863
references: J Immunol. 1961 Nov;87:499-503
references: 13881115
references: Trends Cell Biol. 2005 Feb;15(2):84-91
references: 15695095
references: Annu Rev Biochem. 2006;75:743-67
references: 16756510
references: Science. 1977 Jan 21;195(4275):307-9
references: 556653
references: Biophys J. 2011 Dec 21;101(12):2855-70
references: 22208184
references: Biophys Chem. 1979 Sep;10(2):221-9
references: 16997218
references: Biochim Biophys Acta. 1974 Nov 15;367(3):282-94
references: 4429678