title: Manipulating circadian clock neuron firing rate resets molecular circadian rhythms and behavior.
abstract: To examine the interaction between molecular, electrical and behavioral circadian rhythms, we combined optogenetic manipulation of suprachiasmatic nucleus (SCN) firing rate with bioluminescence imaging and locomotor activity monitoring. Manipulating firing rate reset circadian rhythms both ex vivo and in vivo, and this resetting required spikes and network communication. This suggests that SCN firing rate is fundamental to circadian pacemaking as both an input to and output of the molecular clockworks.
authors: Neuroscience Graduate Program, Vanderbilt University, Nashville, Tennessee, USA.
authors: Jeff R
authors: 0000000283867798
authors: JR
authors: Jones
authors: Neuroscience Graduate Program, Vanderbilt University, Nashville, Tennessee, USA.
authors: Michael C
authors: MC
authors: Tackenberg
authors: 1] Neuroscience Graduate Program, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
authors: Douglas G
authors: DG
authors: McMahon
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000075402:Channelrhodopsins; D002940:Circadian Rhythm / Q000502:physiology*; D066298:In Vitro Techniques; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009043:Motor Activity / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism; D010775:Photic Stimulation; D017447:Receptors, Dopamine D1 / Q000235:genetics / Q000378:metabolism; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013493:Suprachiasmatic Nucleus / Q000166:cytology*; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D000075402:Channelrhodopsins; C583261:Drd1 protein, mouse; D008164:Luminescent Proteins; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D017447:Receptors, Dopamine D1; D026941:Sodium Channel Blockers; C048507:vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin
references: Science. 2003 Nov 21;302(5649):1408-12
references: 14631044
references: Neuron. 2013 May 22;78(4):714-28
references: 23623697
references: J Biol Rhythms. 2006 Dec;21(6):470-81
references: 17107937
references: Proc Natl Acad Sci U S A. 1987 Mar;84(6):1694-8
references: 3470750
references: PLoS One. 2010 Sep 07;5(9):e12612
references: 20830308
references: Brain Res Rev. 2006 Jun;51(1):1-60
references: 16337005
references: J Neurosci. 2007 Oct 31;27(44):12078-87
references: 17978049
references: Neuron. 2013 Nov 20;80(4):973-83
references: 24267653
references: Neuroscience. 2014 Feb 28;260:249-64
references: 24355494
references: Cell. 2002 May 17;109(4):485-95
references: 12086605
references: J Neural Eng. 2007 Sep;4(3):S143-56
references: 17873414
references: Neuron. 2013 Jun 5;78(5):799-806
references: 23764285
references: Annu Rev Physiol. 2010;72:551-77
references: 20148688
references: PLoS One. 2012;7(6):e38985
references: 22701739
references: Neuron. 1995 Apr;14(4):697-706
references: 7718233
references: Neuron. 2011 Jul 14;71(1):9-34
references: 21745635
references: Nat Rev Neurosci. 2011 Sep 02;12(10):553-69
references: 21886186
references: J Neurophysiol. 1999 Aug;82(2):533-9
references: 10444653
references: Neuron. 2010 Nov 4;68(3):512-28
references: 21040851
references: Nat Neurosci. 1998 Dec;1(8):708-13
references: 10196587
references: J Neurophysiol. 2013 Sep;110(5):1097-106
references: 23741043
references: PLoS Biol. 2008 Oct 14;6(10):e249
references: 18959477
references: Science. 1994 Dec 9;266(5191):1713-7
references: 7527589
references: J Neurosci. 2003 Feb 15;23(4):1441-50
references: 12598633