title: Infectious Maize rayado fino virus from Cloned cDNA.
abstract: A full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur.
authors: First and second authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Cereal Crops Research Unit, Fargo, ND 58102-2765; third and fourth authors: USDA-ARS Corn, Soybean, and Wheat Quality Research Unit, Wooster, OH 44691.
authors: Michael C
authors: MC
authors: Edwards
authors: First and second authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Cereal Crops Research Unit, Fargo, ND 58102-2765; third and fourth authors: USDA-ARS Corn, Soybean, and Wheat Quality Research Unit, Wooster, OH 44691.
authors: John J
authors: JJ
authors: Weiland
authors: First and second authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Cereal Crops Research Unit, Fargo, ND 58102-2765; third and fourth authors: USDA-ARS Corn, Soybean, and Wheat Quality Research Unit, Wooster, OH 44691.
authors: Jane
authors: J
authors: Todd
authors: First and second authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Cereal Crops Research Unit, Fargo, ND 58102-2765; third and fourth authors: USDA-ARS Corn, Soybean, and Wheat Quality Research Unit, Wooster, OH 44691.
authors: Lucy R
authors: LR
authors: Stewart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D046830:Tymoviridae / Q000235:genetics / Q000302:isolation & purification*; D003313:Zea mays / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D018076:DNA, Complementary; D012367:RNA, Viral