title: Proteomic analysis of Streptomyces sp. ssr-198 grown on paddy straw.
abstract: The filamentous bacteria Streptomyces spp. produces diverse extracellular enzymes and other secondary metabolites. Proteomic analysis of the secretome of holocellulolytic Streptomyces sp. ssr-198 was done by tandem mass spectrometry using an Orbitrap Velos hybrid mass spectrometer. A wide range of hydrolytic enzymes, including glycoside hydrolases (17), proteases (17), polysaccharide lyases (3), esterases (2), and hypothetical proteins (14) were detected in the secretome analyzed. Overall, the secretome composition constituted of 12.50% cellulases, 17.50% hemicellulases, 21.25% proteases, 17.50% hypothetical proteins, and 31.25% other proteins. Comprehensive analysis of secretome will be useful in gaining better understanding of the unique role of hydrolytic enzymes in lignocellulose hydrolysis and helps in determining the industrial applications of these potent enzymes.
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
authors: Surender
authors: S
authors: Singh
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
authors: Rameshwar
authors: R
authors: Tiwari
authors: Institute of Bioinformatics, International Technology Park, Bangalore, Karnataka, India.
authors: Santosh
authors: S
authors: Renuse
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
authors: Kumar
authors: K
authors: Pranaw
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
authors: Lata
authors: L
authors: Nain
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D044602:Cellulases / Q000032:analysis / Q000378:metabolism; D006867:Hydrolases / Q000032:analysis* / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D013302:Streptomyces / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; C036909:lignocellulose; D008031:Lignin; D006867:Hydrolases; D044602:Cellulases
keywords: Bioethanol; Holocellulase; Orbitrap; Paddy straw; Streptomyces