title: Characterization ofAspergillus aculeatusβ-glucosidase 1 accelerating cellulose hydrolysis withTrichodermacellulase system.
abstract: Aspergillus aculeatusβ-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis byTrichodermacellulase system, was characterized and compared some properties to a commercially supplied orthologue inA. niger(AnBGL) to elucidate advantages of recombinant AaBGL1 (rAaBGL1) for synergistic effect onTrichodermaenzymes. Steady-state kinetic studies revealed that rAaBGL1 showed high catalytic efficiency towards β-linked glucooligosaccharides. Up to a degree of polymerization (DP) 3, rAaBGL1 prefered to hydrolyze β-1,3 linked glucooligosaccharides, but longer than DP 3, preferred β-1,4 glucooligosaccharides (up to DP 5). This result suggested that there were different formation for subsites in the catalytic cleft of AaBGL1 between β-1,3 and β-1,4 glucooligosaccharides, therefore rAaBGL1 preferred short chain of laminarioligosaccharides and long chain of cellooligosaccharides on hydrolysis. rAaBGL1 was more insensitive to glucose inhibition and more efficient to hydrolyze the one of major transglycosylation product, gentiobiose than AnBGL, resulting that rAaBGL1 completely hydrolyzed 5% cellobiose to glucose faster than AnBGL. These data indicate that AaBGL1 is valuable for the use of cellulosic biomass conversion.
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 Japan.
authors: Yutaro
authors: Y
authors: Baba
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 Japan.
authors: Jun-Ichi
authors: JI
authors: Sumitani
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 Japan.
authors: Shuji
authors: S
authors: Tani
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 Japan.
authors: Takashi
authors: T
authors: Kawaguchi
keywords: Aspergillus aculeatus; Biomass conversion; Cellulase; Trichoderma reesei; β-glucosidase
references: J Ind Microbiol Biotechnol. 2012 Dec;39(12):1741-9
references: 23053344
references: J Biosci Bioeng. 2003;95(6):572-6
references: 16233459
references: Proteins. 2000 Nov 1;41(2):257-69
references: 10966578
references: Appl Biochem Biotechnol. 2010 May;161(1-8):313-7
references: 19830597
references: Carbohydr Res. 2004 Dec 27;339(18):2851-7
references: 15582611
references: Biochem J. 1993 Aug 1;293 ( Pt 3):781-8
references: 8352747
references: Bioresour Technol. 2013 Nov;148:105-13
references: 24045198
references: Bioresour Technol. 2013 Jan;127:500-7
references: 23069613
references: Protein J. 2004 Jan;23(1):11-23
references: 15115178
references: Biotechnol Bioeng. 2007 Jun 1;97(2):287-96
references: 17058283
references: Glycoconj J. 1999 Aug;16(8):415-23
references: 10737327
references: Biochim Biophys Acta. 1973 Apr 12;302(2):362-75
references: 4699245
references: Gene. 1996 Sep 16;173(2):287-8
references: 8964516
references: Biochem J. 1996 Jun 1;316 ( Pt 2):695-6
references: 8687420
references: Appl Environ Microbiol. 2010 Oct;76(20):6760-8
references: 20729316
references: Carbohydr Res. 1997 Feb 20;298(1-2):51-7
references: 9076931
references: Biosci Biotechnol Biochem. 1998 Oct;62(10):1938-46
references: 9836430
references: J Biol Chem. 2000 Feb 18;275(7):4973-80
references: 10671536
references: Appl Microbiol Biotechnol. 2011 Aug;91(4):1073-82
references: 21626020
references: Biotechnol Bioeng. 2012 Jan;109(1):92-9
references: 21830204
references: Appl Biochem Biotechnol. 2004 Spring;113-116:1115-26
references: 15054257
references: J Biosci Bioeng. 2001;92(2):131-7
references: 16233072
references: Biochem J. 2013 Jun 1;452(2):211-21
references: 23537284
references: Biotechnol Biofuels. 2013 Jan 28;6(1):8
references: 23356733
references: Biotechnol Bioeng. 1986 Sep;28(9):1438-42
references: 18561233
references: Biotechnol Adv. 2010 May-Jun;28(3):308-24
references: 20080173
references: Microb Cell Fact. 2012 May 20;11:63
references: 22607229
references: Plant Cell. 2002 May;14(5):1033-52
references: 12034895
references: Biochem J. 1991 Dec 1;280 ( Pt 2):309-16
references: 1747104
references: Biochim Biophys Acta. 2006 May;1764(5):972-8
references: 16650812
references: Appl Microbiol Biotechnol. 2012 Mar;93(5):1989-98
references: 21850431
references: Eur J Biochem. 1992 Oct 15;209(2):651-9
references: 1425671
references: Biotechnol Biofuels. 2010 Feb 11;3(1):3
references: 20181208
references: Appl Environ Microbiol. 1999 Dec;65(12):5546-53
references: 10584016