title: Association of TNF -1031 C/C as a potential protection marker for leprosy development in Amazonas state patients, Brazil.
abstract: Polymorphisms present in the TNF promoter region has shown to influence the gene transcription. Leprosy displays different clinical manifestations according to the immune responses of the individual infected with Mycobacterium leprae. In this study, we evaluated the single nucleotide polymorphisms (SNPs) -238 G/A (rs361525), -308 G/A (rs1800629), -857 C/T (rs1799724), -863 A/C (rs1800630) and -1031 T/C (rs1799964) in the promoter region of the TNF to see whether these SNPs influence host-susceptibility to leprosy and the different clinical manifestation. Nucleotide sequencing was performed with DNA samples from 108 leprosy patients and 253 control subjects. An association between -1031 C/C genotype and protection from leprosy was observed when leprosy patients were compared to controls (OR 0.11; 95% CI=0.01-0.82; p=0.012). The -857 C/T genotype may be associated with susceptibility to leprosy (OR=1.81; 95% CI=1.09-3.00; p=0.028). Similar genotype and allele frequencies for the SNPs -308 G/A and -238 G/A were observed between leprosy patients and control subjects. Altogether, TNF polymorphisms in the promoter region may be predictive of leprosy outcome.
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil. Electronic address: georgevillarouco@hotmail.com.
authors: G A V
authors: GA
authors: Silva
authors: Fundação de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus, Amazonas, Brazil; Faculdade de Medicina, Universidade Nilton Lins, Manaus, Amazonas, Brazil.
authors: R
authors: R
authors: Ramasawmy
authors: Laboratório de Imunologia, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, Brazil.
authors: A L
authors: AL
authors: Boechat
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil.
authors: A C
authors: AC
authors: Morais
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil.
authors: B K S
authors: BK
authors: Carvalho
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil.
authors: K B A
authors: KB
authors: Sousa
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil.
authors: V C
authors: VC
authors: Souza
authors: Fundação de Dermatologia e Venereologia Alfredo da Matta, Manaus, Amazonas, Brazil.
authors: M G S
authors: MG
authors: Cunha
authors: Laboratório de Micobacteriologia, Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil.
authors: R H
authors: RH
authors: Barletta-Naveca
authors: Laboratório de Micobacteriologia, Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil.
authors: M P
authors: MP
authors: Santos
authors: Instituto Leônidas e Maria Deane, FIOCRUZ Amazônia, Manaus, Amazonas, Brazil.
authors: F G
authors: FG
authors: Naveca
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D004252:DNA Mutational Analysis; D018450:Disease Progression; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007918:Leprosy / Q000175:diagnosis / Q000235:genetics / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha
keywords: Genetic marker; Leprosy; Polymorphism; TNF