title: Interferon γ-induced GTPase promotes invasion of Listeria monocytogenes into trophoblast giant cells.
abstract: Listeria monocytogenes is well known for having the ability to cross the placental barrier, leading to fetal infections and abortion. However, the mechanisms leading to infectious abortion are poorly understood. In this study, we demonstrate that interferon γ-induced GTPase (IGTP) contributes to the invasion of L. monocytogenes into trophoblast giant (TG) cells, which are placental immune cells. Knockdown of IGTP in TG cells decreased the relative efficiencies of L. monocytogenes invasion. Moreover, IGTP accumulated around infected L. monocytogenes in TG cells. Treatment of TG cells with phosphatidylinositol 3-kinase (PI3K)/Akt inhibitors also reduced bacterial invasion. PI3K/Akt inhibitor or IGTP knockdown reduced the amount of phosphorylated Akt. Monosialotetrahexosylganglioside (GM1) gangliosides, lipid raft markers, accumulated in the membrane of L. monocytogenes-containing vacuoles in TG cells. Furthermore, treatment with a lipid raft inhibitor reduced bacterial invasion. These results suggest that IGTP-induced activation of the PI3K/Akt signaling pathway promotes bacterial invasion into TG cells.
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Masato
authors: M
authors: Tachibana
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Masanori
authors: M
authors: Hashino
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Kenta
authors: K
authors: Watanabe
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Takashi
authors: T
authors: Shimizu
authors: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors: Masahisa
authors: M
authors: Watarai
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000472:pathogenicity*; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D020558:GTP Phosphohydrolases; D007371:Interferon-gamma; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; C101131:Igtp protein, mouse
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