title: A novel recombinant bivalent outer membrane protein of Vibrio vulnificus and Aeromonas hydrophila as a vaccine antigen of American eel (Anguilla rostrata).
abstract: The immogenicity of a novel vaccine antigen was evaluated after immunized American eels (Anguilla rostrata) with a recombinant bivalent expressed outer membrane protein (OMP) of Vibrio vulnificus and Aeromonas hydrophila. Three groups of eels were intraperitoneal (i.p) injected with phosphate-buffered saline (PBS group), formaline-killed-whole-cell (FKC) of A. hydrophila and V. vulnificus (FKC group) or the bivalent OMP (OMP group). On 14, 21, 28 and 42 days post-vaccination respectively, proliferation of the whole blood cells, titers of specific antibody and lysozyme activities of experimental eels were detected. On 28 day post-vaccination, eels from three groups were challenged by i.p injection of live A. hydrophila or V. vulnificus. The results showed that, compared with the PBS group, proliferation of whole blood cells in OMP group was significant enhanced on 28 days, and the serum titers of anti-A.hydrophila and anti-V. vulnificus antibody in eels of FKC and OMP group were significant increased on 14, 21 and 28d. Lysozyme Activities in serum, skin mucus, liver and kidney were significant changed between the three groups. Relative Percent Survival (RPS) after challenged A. hydrophila in KFC vs. PBS group and OMP vs. PBS group were 62.5% and 50% respectively, and the RPS challenged V. vulnificus in FKC and OMP vs. PBS group were 37.5% and 50% respectively. These results suggest that American eels immunized with the bivalent OMP would positively affect specific as well as non-specific immune parameters and protect against infection by the two pathogens in fresh water farming.
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China. Electronic address: gsl@jmu.edu.cn.
authors: Guo
authors: G
authors: SongLin
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China.
authors: Lu
authors: L
authors: PanPan
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China.
authors: Feng
authors: F
authors: JianJun
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China.
authors: Zhao
authors: Z
authors: JinPing
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China.
authors: Lin
authors: L
authors: Peng
authors: Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China.
authors: Duan
authors: D
authors: LiHua
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D016980:Aeromonas hydrophila / Q000276:immunology; D000811:Anguilla; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D018272:Porins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D041261:Vibrio vulnificus / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018272:Porins; D011994:Recombinant Proteins; C406585:porin II protein, Aeromonas
keywords: Aeromonas hydrophila; Bivalent expressed OMP; Vaccine antigen; Vibrio vulnificus