title: In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments.
abstract: Surface modification treatments can be used to improve the biocompatibility of austenitic stainless steels. In the present research two different modifications of AISI 316L stainless steel were considered, low temperature nitriding and collagen-I coating, applied as single treatment or in conjunction. Low temperature nitriding produced modified surface layers consisting mainly of S phase, which enhanced corrosion resistance in PBS solution. Biocompatibility was assessed using human peripheral blood mononuclear cells (PBMC) in culture. Proliferation, lactate dehydrogenase (LDH) levels, release of cytokines (TNF-α, IL-1β, IL-12, IL-10), secretion of metalloproteinase (MMP)-9 and its inhibitor TIMP-1, and the gelatinolytic activity of MMP-9 were determined. While the 48-h incubation of PBMC with all the sample types did not negatively influence cell proliferation, LDH and MMP-9 levels, suggesting therefore a good biocompatibility, the release of the pro-inflammatory cytokines was always remarkable when compared to that of control cells. However, in the presence of the nitrided and collagen coated samples, the release of the pro-inflammatory cytokine IL-1β decreased, while that of the anti-inflammatory cytokine IL-10 increased, in comparison with the untreated AISI 316L samples. Our results suggest that some biological parameters were ameliorated by these surface treatments of AISI 316L.
authors: Department of Biomedical, Experimental and Clinical Sciences 'Mario Serio', Section of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134, Florence, Italy.
authors: Maria
authors: M
authors: Stio
authors: Maria
authors: M
authors: Martinesi
authors: Cristina
authors: C
authors: Treves
authors: Francesca
authors: F
authors: Borgioli
mesh_terms: D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003343:Corrosion; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism*; D008422:Materials Testing; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D016207:Cytokines; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; C426023:austenitic steel; D013193:Stainless Steel; D003094:Collagen; D007770:L-Lactate Dehydrogenase; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D009584:Nitrogen