title: Generation of transgenic mouse model using PTTG as an oncogene.
abstract: The close physiological similarity between the mouse and human has provided tools to understanding the biological function of particular genes in vivo by introduction or deletion of a gene of interest. Using a mouse as a model has provided a wealth of resources, knowledge, and technology, helping scientists to understand the biological functions, translocation, trafficking, and interaction of a candidate gene with other intracellular molecules, transcriptional regulation, posttranslational modification, and discovery of novel signaling pathways for a particular gene. Most importantly, the generation of the mouse model for a specific human disease has provided a powerful tool to understand the etiology of a disease and discovery of novel therapeutics. This chapter describes in detail the step-by-step generation of the transgenic mouse model, which can be helpful in guiding new investigators in developing successful models. For practical purposes, we will describe the generation of a mouse model using pituitary tumor transforming gene (PTTG) as the candidate gene of interest.
authors: Department of Physiology and Biophysics, and James Graham Brown Cancer Center, University of Louisville, 580 South Preston Street, Baxter II, Room 321E, Louisville, KY, 40202, USA, sham.kakar@louisville.edu.
authors: Sham S
authors: SS
authors: Kakar
authors: Cohin
authors: C
authors: Kakar
mesh_terms: D000818:Animals; D001947:Breeding; D063646:Carcinogenesis; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D018703:Founder Effect; D005818:Genetic Engineering / Q000379:methods*; D060005:Genotyping Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D018146:Muromegalovirus / Q000235:genetics; D009857:Oncogenes / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D064249:Securin / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D015053:Zygote / Q000378:metabolism
chemical_list: D064249:Securin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA