title: Protective effect of sodium nitroprusside on the rat small intestine transplanted mucosa.
abstract: The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reported in vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na(+)-K(+)-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.
authors: Ultrasound Department, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China.
authors: Feng-Hua
authors: 0000-0003-3309-3787
authors: FH
authors: Chen
authors: Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China.
authors: Ke
authors: 0000-0003-3131-4427
authors: K
authors: Li
authors: Shanghai Institute of Digestive Surgery, Shanghai Jiaotong University Medical College, Shanghai 200025, China.
authors: Lu
authors: L
authors: Yin
authors: Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China.
authors: Chun-Qiu
authors: 0000-0002-3594-9692
authors: CQ
authors: Chen
authors: Department of Pathology, Shanghai Jiaotong University Medical College, Shanghai 200025, China.
authors: Zhao-Wen
authors: ZW
authors: Yan
authors: Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China.
authors: Gui-Ming
authors: 0000-0002-3433-4987
authors: GM
authors: Chen
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