title: Dosage Effects of Salt and pH Stresses onSaccharomyces cerevisiaeas MonitoredviaMetabolites by Using Two Dimensional NMR Spectroscopy.
abstract: Saccharomyces cerevisiae, which is a common species of yeast, is by far the most extensively studied model of a eukaryote because although it is one of the simplest eukaryotes, its basic cellular processes resemble those of higher organisms. In addition, yeast is a commercially valuable organism for ethanol production. Since the yeast data can be extrapolated to the important aspects of higher organisms, many researchers have studied yeast metabolism under various conditions. In this report, we analyzed and compared metabolites ofSaccharomyces cerevisiaeunder salt and pH stresses of various strengths by using two-dimensional NMR spectroscopy. A total of 31 metabolites were identified for most of the samples. The levels of many identified metabolites showed gradual or drastic increases or decreases depending on the severity of the stresses involved. The statistical analysis produced a holistic outline: pH stresses were clustered together, but salt stresses were spread out depending on the severity. This work could provide a link between the metabolite profiles and mRNA or protein profiles under representative and well studied stress conditions.
authors: Department of Chemistry, Sejong University, Seoul 143-747, Korea.
authors: Young Kee
authors: YK
authors: Chae
authors: Department of Chemistry, Sejong University, Seoul 143-747, Korea.
authors: Seol Hyun
authors: SH
authors: Kim
authors: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors: James E
authors: JE
authors: Ellinger
authors: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors: John L
authors: JL
authors: Markley
keywords: Metabolite profiling; NMR; Stress; Yeast
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