title: Functional analysis of the uropathogenic Escherichia coli R049 gene.
abstract: The objective of this study was to determine the function of the novel uropathogenic Escherichia coli (UPEC) gene R049 during host infection. We infected the urinary tracts of mice with E. coli UPEC132 or the R049 deletion mutant UPEC132ΔR049.The mouse kidneys were harvested at 4 and 8h post-infection and screened for differentially expressed genes by microarray analysis. We identified 379 and 515 differentially expressed genes at 4 and 8 h post-infection, respectively. Thirty-four of these genes were associated with inflammatory and immune signaling pathways, including those related to mitogen-activated protein kinase signaling, leukocyte transendothelial migration, cytokine-cytokine receptor interaction, Toll-like receptor signaling, and apoptosis. Protein binding (GO 0005515) was the most prevalent molecular function in the Gene Ontology terms related to differentially expressed genes. In conclusion, R049 expression in UPEC132 is related to the early innate immune and inflammatory responses in UPEC-infected hosts. This work lays the foundation for further research on anti-infective immunity against UPEC.
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China. Electronic address: yangdongjing@cdctj.gov.cn.
authors: Dongjing
authors: D
authors: Yang
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Jie
authors: J
authors: Dong
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Xu
authors: X
authors: Su
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Wei
authors: W
authors: Zhang
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Li
authors: L
authors: Zhang
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Li
authors: L
authors: Li
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Likun
authors: L
authors: Lv
authors: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors: Liru
authors: L
authors: Guo
mesh_terms: D000818:Animals; D058491:Bacterial Load; D019295:Computational Biology; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D007668:Kidney / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome; D014552:Urinary Tract Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins
keywords: GO analysis; Gene expression profile; KEGG pathway analysis; Uropathogenic Escherichia coli