title: Isolation and quality control of functional mitochondria.
abstract: Numerous protocols are available being adapted for different cell or tissue types allowing isolation of pure mitochondria trying to preserve their "structural and functional" integrity. In this chapter we intend to provide a more general framework introducing differential isopycnic density gradient centrifugation strategy with a special focus sensitizing for the specific challenges coming along with this method and how to obtain "functional," enriched, "intact" mitochondria. Due to the fact that in any study dealing with these organelles standardized processing is mandatory. Here we describe a strategy addressing quality control of prepared intact mitochondria. The quality control should be an integrated part of all isolation processes. The underlying protocol should be seen as starting point and has to be carefully adjusted to cover different sample types used for the diverse research questions.
authors: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf, Leibniz Center for Diabetes Research, Auf'm Hennekamp 65, 40225, Duesseldorf, Germany.
authors: Sonja
authors: S
authors: Hartwig
authors: Jorg
authors: J
authors: Kotzka
authors: Stefan
authors: S
authors: Lehr
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods* / Q000592:standards*; D002499:Centrifugation, Density Gradient / Q000379:methods / Q000592:standards; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism; D011786:Quality Control
chemical_list: D004798:Enzymes; D024101:Mitochondrial Proteins