title: Direct analysis of six antibiotics in wastewater samples using rapid high-performance liquid chromatography coupled with diode array detector: a chemometric study towards green analytical chemistry.
abstract: In this work, a rapid HPLC-DAD method has been developed for the analysis of six antibiotics (amoxicillin, metronidazole, sulfamethoxazole, ofloxacine, sulfadiazine and sulfamerazine) in the sewage treatment plant influent and effluent samples. Decreasing the chromatographic run time to less than 4 min as well as lowering the cost per analysis, were achieved through direct injection of the samples into the HPLC system followed by chemometric analysis. The problem of the complete separation of the analytes from each other and/or from the matrix ingredients was resolved as a posteriori. The performance of MCR/ALS and U-PLS/RBL, as second-order algorithms, was studied and comparable results were obtained from implication of these modeling methods. It was demonstrated that the proposed methods could be used promisingly as green analytical strategies for detection and quantification of the targeted pollutants in wastewater samples while avoiding the more complicated high cost instrumentations.
authors: Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran. Electronic address: vosough@ccerci.ac.ir.
authors: Maryam
authors: M
authors: Vosough
authors: Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran.
authors: Masoumeh
authors: M
authors: Rashvand
authors: Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran.
authors: Hadi M
authors: HM
authors: Esfahani
authors: Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran.
authors: Kazem
authors: K
authors: Kargosha
authors: Environmental Sciences Research Institute, Shahid Beheshti University, G.C., Tehran, Iran.
authors: Amir
authors: A
authors: Salemi
mesh_terms: D000658:Amoxicillin / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055772:Green Chemistry Technology; D008795:Metronidazole / Q000032:analysis; D015242:Ofloxacin / Q000032:analysis; D013411:Sulfadiazine / Q000032:analysis; D013416:Sulfamerazine / Q000032:analysis; D013420:Sulfamethoxazole / Q000032:analysis; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000658:Amoxicillin; D000900:Anti-Bacterial Agents; D008795:Metronidazole; D015242:Ofloxacin; D013411:Sulfadiazine; D013416:Sulfamerazine; D013420:Sulfamethoxazole; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Antibiotics; Direct analysis; HPLC–DAD; Second-order calibration; Wastewater