title: Selective removal of ATP degradation products from food matrices II: Rapid screening of hypoxanthine and inosine by molecularly imprinted matrix solid-phase dispersion for evaluation of fish freshness.
abstract: A water compatible molecularly imprinted polymer (MIP), synthesized using theophylline (TPH) as dummy-template and acrylamide (AM) as functional monomer, has been employed as supporting material in matrix solid-phase dispersion combined with ultra performance liquid chromatography-photodiode array detection (MSPD-UPLC-PDA) for selective determination of adenosine triphosphate (ATP) derivatives in fish samples. ATP degradation products are used as freshness index for assessment of fish quality. The solid sample was directly blended with MIP in MSPD procedure resulting in sample disruption and subsequent adsorption of the compounds on the MIP. By using n-hexane and ammonium hydroxide aqueous solution at pH 9 as the washing and elution solvent, respectively, satisfactory recoveries and clean chromatograms have been obtained. Good linearity for hypoxanthine (HYP) and inosine (INO) has been observed with correlation coefficients (R(2)) of 0.9987 and 0.9986, respectively. The recoveries of the two ATP derivatives at three different spiked levels ranged from 106.5% to 113.4% for HYP and from 103.1% to 111.2% for INO, with average relative standard deviations lower than 4.2% in both cases. This new method, which is rapid, simple and sensitive, can be used as an alternative tool to conventional tedious methods.
authors: Grupo de Polímeros, Centro de Investigaciones Tecnológicas (CIT), Departamento de Física, Escuela Universitaria Politécnica, Universidad de A Coruña (UDC), Campus de Ferrol, 15471 Ferrol, Spain.
authors: M C
authors: MC
authors: Cela-Pérez
authors: Departamento de Ingeniería Química, Escuela de Ingeniería Industrial (EEI), Universidad de de Vigo, Campus As Lagoas, Marcosende 36310, Vigo-Pontevedra, Spain.
authors: L
authors: L
authors: Barbosa-Pereira
authors: Departamento de Ingeniería Química, Escuela de Ingeniería Industrial (EEI), Universidad de de Vigo, Campus As Lagoas, Marcosende 36310, Vigo-Pontevedra, Spain.
authors: X
authors: X
authors: Vecino
authors: Departamento de Ingeniería Química, Escuela de Ingeniería Industrial (EEI), Universidad de de Vigo, Campus As Lagoas, Marcosende 36310, Vigo-Pontevedra, Spain.
authors: M
authors: M
authors: Pérez-Ameneiro
authors: Grupo de Polímeros, Centro de Investigaciones Tecnológicas (CIT), Departamento de Física, Escuela Universitaria Politécnica, Universidad de A Coruña (UDC), Campus de Ferrol, 15471 Ferrol, Spain; Departamento de Química Orgánica I, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, Arcos de Jalón no. 118, Madrid 28037, Spain.
authors: Aurora
authors: A
authors: Lasagabaster Latorre
authors: Grupo de Polímeros, Centro de Investigaciones Tecnológicas (CIT), Departamento de Física, Escuela Universitaria Politécnica, Universidad de A Coruña (UDC), Campus de Ferrol, 15471 Ferrol, Spain.
authors: J M
authors: JM
authors: López-Vilariño
authors: Grupo de Polímeros, Centro de Investigaciones Tecnológicas (CIT), Departamento de Física, Escuela Universitaria Politécnica, Universidad de A Coruña (UDC), Campus de Ferrol, 15471 Ferrol, Spain. Electronic address: victoria.gonzalez.rodriguez@udc.es.
authors: M V
authors: MV
authors: González Rodríguez
authors: Departamento de Ingeniería Química, Escuela de Ingeniería Industrial (EEI), Universidad de de Vigo, Campus As Lagoas, Marcosende 36310, Vigo-Pontevedra, Spain.
authors: A B
authors: AB
authors: Moldes
authors: Departamento de Ingeniería Química, Escuela de Ingeniería Industrial (EEI), Universidad de de Vigo, Campus As Lagoas, Marcosende 36310, Vigo-Pontevedra, Spain.
authors: J M
authors: JM
authors: Cruz
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D005504:Food Analysis; D063427:Food Quality; D048388:Gadiformes; D019271:Hypoxanthine / Q000032:analysis*; D007288:Inosine / Q000032:analysis*; D054802:Molecular Imprinting
chemical_list: D019271:Hypoxanthine; D007288:Inosine; D000255:Adenosine Triphosphate
keywords: Fish samples; Freshness; Hypoxanthine; Inosine; Matrix solid-phase dispersion; Molecularly imprinted polymer