title: Asymmetric inheritance of the apical domain and self-renewal of retinal ganglion cell progenitors depend on Anillin function.
abstract: Divisions that generate one neuronal lineage-committed and one self-renewing cell maintain the balance of proliferation and differentiation for the generation of neuronal diversity. The asymmetric inheritance of apical domains and components of the cell division machinery has been implicated in this process, and might involve interactions with cell fate determinants in regulatory feedback loops of an as yet unknown nature. Here, we report the dynamics of Anillin - an essential F-actin regulator and furrow component - and its contribution to progenitor cell divisions in the developing zebrafish retina. We find that asymmetrically dividing retinal ganglion cell progenitors position the Anillin-rich midbody at the apical domain of the differentiating daughter. anillin hypomorphic conditions disrupt asymmetric apical domain inheritance and affect daughter cell fate. Consequently, the retinal cell type composition is profoundly affected, such that the ganglion cell layer is dramatically expanded. This study provides the first in vivo evidence for the requirement of Anillin during asymmetric neurogenic divisions. It also provides insights into a reciprocal regulation between Anillin and the ganglion cell fate determinant Ath5, suggesting a mechanism whereby the balance of proliferation and differentiation is accomplished during progenitor cell divisions in vivo.
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Alessio
authors: A
authors: Paolini
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Anne-Laure
authors: AL
authors: Duchemin
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Shahad
authors: S
authors: Albadri
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany Department of Ophthalmology, University of Heidelberg, Heidelberg 69120, Germany.
authors: Eva
authors: E
authors: Patzel
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Dorothee
authors: D
authors: Bornhorst
authors: Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Paula
authors: P
authors: González Avalos
authors: Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
authors: Steffen
authors: S
authors: Lemke
authors: Biotechnology Center and Center for Regenerative Therapies Dresden, TU Dresden, Fetscherstrasse 105, Dresden 01307, Germany.
authors: Anja
authors: A
authors: Machate
authors: Biotechnology Center and Center for Regenerative Therapies Dresden, TU Dresden, Fetscherstrasse 105, Dresden 01307, Germany.
authors: Michael
authors: M
authors: Brand
authors: Department of Ophthalmology, University of Heidelberg, Heidelberg 69120, Germany.
authors: Saadettin
authors: S
authors: Sel
authors: Institut Curie - Centre de Recherche, U934/UMR3215, Paris 75248, Cedex 05, France.
authors: Vincenzo
authors: V
authors: Di Donato
authors: Institut Curie - Centre de Recherche, U934/UMR3215, Paris 75248, Cedex 05, France.
authors: Filippo
authors: F
authors: Del Bene
authors: Sección Biología Celular, Facultad de Ciencias, Universidad de la República and Institut Pasteur de Montevideo, 11400 Montevideo, Uruguay.
authors: Flavio R
authors: FR
authors: Zolessi
authors: Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria 3187, Australia.
authors: Mirana
authors: M
authors: Ramialison
authors: Department of Developmental Biology/Physiology, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany lucia.poggi@cos.uni-heidelberg.de.
authors: Lucia
authors: L
authors: Poggi
mesh_terms: D000818:Animals; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003285:Contractile Proteins; D029961:Zebrafish Proteins; C095886:anillin
keywords: Anillin; Apical domain inheritance; Asymmetric cell division; Ath5 (Atoh7); Neurogenesis; Retina development