title: The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione.
abstract: Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2-oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2-oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an excess of glutathione did not compete for substrate uptake nor could transport of glutathione be measured directly. Thus these mitochondrial carriers do not transport glutathione and the identity of the mitochondrial glutathione transporter remains unknown.
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors: Lee M
authors: LM
authors: Booty
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors: Martin S
authors: MS
authors: King
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors: Chancievan
authors: C
authors: Thangaratnarajah
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors: Homa
authors: H
authors: Majd
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.
authors: Andrew M
authors: AM
authors: James
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK. Electronic address: ek@mrc-mbu.cam.ac.uk.
authors: Edmund R S
authors: ER
authors: Kunji
authors: Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK. Electronic address: mpm@mrc-mbu.cam.ac.uk.
authors: Michael P
authors: MP
authors: Murphy
mesh_terms: D029382:Dicarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D015151:Immunoblotting; D007656:Ketoglutaric Acids / Q000378:metabolism; D013294:Lactococcus lactis / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D029382:Dicarboxylic Acid Transporters; D007656:Ketoglutaric Acids; D026901:Membrane Transport Proteins; D017382:Reactive Oxygen Species; C021723:oxoglutarate translocator; D005978:Glutathione
keywords: Competitive inhibition; Glutathione transport; Lactococcus lactis; Metabolite exchange; Mitochondrial carriers; Reactive oxygen species
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