title: Characterization of mitochondrial populations during stem cell differentiation.
abstract: Mitochondrial dynamics play an important role in numerous physiological and pathophysiological phenomena in the developing and adult human heart. Alterations in structural aspects of cellular mitochondrial composition as a function of changes in physiology can easily be visualized using fluorescence microscopy. Commonly, mitochondrial location, number, and morphology are reported qualitatively due to the lack of automated and user-friendly computer-based analysis tools. Mitochondrial Quantification using MATLAB (MQM) is a computer-based tool to quantitatively assess these parameters by analyzing fluorescently labeled mitochondria within the cell; in particular, MQM provides numerical information on the number, area, and location of mitochondria within a cell in a time-efficient, automated, and unbiased way. This chapter describes the use of MQM's capabilities to quantify mitochondrial changes during human pluripotent stem cell (hPSC) differentiation into spontaneously contracting cardiomyocytes (SC-CMs), which follows physiological pathways of human heart development.
authors: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL, 36849, USA.
authors: Petra
authors: P
authors: Kerscher
authors: Blakely S
authors: BS
authors: Bussie
authors: Katherine M
authors: KM
authors: DeSimone
authors: David A
authors: DA
authors: Dunn
authors: Elizabeth A
authors: EA
authors: Lipke
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D007091:Image Processing, Computer-Assisted; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000502:physiology*; D057054:Molecular Imaging / Q000379:methods; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*