title: Globally invading populations of the fungal plant pathogen Verticillium dahliae are dominated by multiple divergent lineages.
abstract: The spread of aggressive fungal pathogens into previously non-endemic regions is a major threat to plant health and food security. Analyses of the spatial and genetic structure of plant pathogens offer valuable insights into their origin, dispersal mechanisms and evolution, and have been useful to develop successful disease management strategies. Here, we elucidated the genetic diversity, population structure and demographic history of worldwide invasion of the ascomycete Verticillium dahliae, a soil-borne pathogen, using a global collection of 1100 isolates from multiple plant hosts and countries. Seven well-differentiated genetic clusters were revealed through discriminant analysis of principal components (DAPC), but no strong associations between these clusters and host/geographic origin of isolates were found. Analyses of clonal evolutionary relationships among multilocus genotypes with the eBURST algorithm and analyses of genetic distances revealed that genetic clusters represented several ancient evolutionary lineages with broad geographic distribution and wide host range. Comparison of different scenarios of demographic history using approximate Bayesian computations revealed the branching order among the different genetic clusters and lineages. The different lineages may represent incipient species, and this raises questions with respect to their evolutionary origin and the factors allowing their maintenance in the same areas and same hosts without evidence of admixture between them. Based on the above findings and the biology of V. dahliae, we conclude that anthropogenic movement has played an important role in spreading V. dahliae lineages. Our findings have implications for the development of management strategies such as quarantine measures and crop resistance breeding.
authors: Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
authors: Dylan P G
authors: DP
authors: Short
authors: Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
authors: Suraj
authors: S
authors: Gurung
authors: Ecologie Systematique Evolution, CNRS, Université Paris Sud, Orsay, F-91405, France.
authors: Pierre
authors: P
authors: Gladieux
authors: Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
authors: Patrik
authors: P
authors: Inderbitzin
authors: Department of Advanced Technology, Hartnell College, Salinas, CA, 93905, USA.
authors: Zahi K
authors: ZK
authors: Atallah
authors: Department of Soil, Plant, and Food Sciences, University of Bari 'Aldo Moro', Bari, 70126, Italy.
authors: Franco
authors: F
authors: Nigro
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Guoqing
authors: G
authors: Li
authors: Adnan Menderes University, Aydin, 09100, Turkey.
authors: Seher
authors: S
authors: Benlioglu
authors: Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
authors: Krishna V
authors: KV
authors: Subbarao
mesh_terms: D001499:Bayes Theorem; D019143:Evolution, Molecular; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D058507:Host Specificity / Q000235:genetics; D058865:Introduced Species; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D020172:Verticillium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification