title: The endosomal protein CHARGED MULTIVESICULAR BODY PROTEIN1 regulates the autophagic turnover of plastids in Arabidopsis.
abstract: Endosomal Sorting Complex Required for Transport (ESCRT)-III proteins mediate membrane remodeling and the release of endosomal intraluminal vesicles into multivesicular bodies. Here, we show that the ESCRT-III subunit paralogs CHARGED MULTIVESICULAR BODY PROTEIN1 (CHMP1A) and CHMP1B are required for autophagic degradation of plastid proteins in Arabidopsis thaliana. Similar to autophagy mutants, chmp1a chmp1b (chmp1) plants hyperaccumulated plastid components, including proteins involved in plastid division. The autophagy machinery directed the release of bodies containing plastid material into the cytoplasm, whereas CHMP1A and B were required for delivery of these bodies to the vacuole. Autophagy was upregulated in chmp1 as indicated by an increase in vacuolar green fluorescent protein (GFP) cleavage from the autophagic reporter GFP-ATG8. However, autophagic degradation of the stromal cargo RECA-GFP was drastically reduced in the chmp1 plants upon starvation, suggesting that CHMP1 mediates the efficient delivery of autophagic plastid cargo to the vacuole. Consistent with the compromised degradation of plastid proteins, chmp1 plastids show severe morphological defects and aberrant division. We propose that CHMP1 plays a direct role in the autophagic turnover of plastid constituents.
authors: Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Christoph
authors: C
authors: Spitzer
authors: Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Faqiang
authors: F
authors: Li
authors: Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Rafael
authors: R
authors: Buono
authors: Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Hannetz
authors: H
authors: Roschzttardtz
authors: Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Taijoon
authors: T
authors: Chung
authors: Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824.
authors: Min
authors: M
authors: Zhang
authors: Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824.
authors: Katherine W
authors: http://orcid.org/0000-0002-0028-2509
authors: KW
authors: Osteryoung
authors: Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors: Richard D
authors: http://orcid.org/0000-0003-0210-3516
authors: RD
authors: Vierstra
authors: Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706 Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin 53706 otegui@wisc.edu.
authors: Marisa S
authors: http://orcid.org/0000-0003-4699-6950
authors: MS
authors: Otegui
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001343:Autophagy; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008954:Models, Biological; D057146:Multivesicular Bodies / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010588:Phagosomes / Q000378:metabolism; D018087:Plastids / Q000378:metabolism / Q000648:ultrastructure*; D021381:Protein Transport; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000603132:CHMP1A protein, Arabidopsis; C000603133:CHMP1B protein, Arabidopsis; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins
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