title: Assessment of short- and medium-chain fatty acids on mitochondrial function in severe inflammation.
abstract: Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The influence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under inflammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under inflammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-α to mimic inflammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR.
authors: University of Giessen and Marburg Lung Center (UGMLC), Justus-Liebig-University of Giessen, Giessen, Germany, Matthias.Hecker@innere.med.uni-giessen.de.
authors: Matthias
authors: M
authors: Hecker
authors: Natascha
authors: N
authors: Sommer
authors: Konstantin
authors: K
authors: Mayer
mesh_terms: D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption; D012720:Severity of Illness Index
chemical_list: D005227:Fatty Acids