title: Adenovirus encoding Smad4 suppresses glioma cell proliferation and increases apoptosis through cell cycle arrest at G1 phase.
abstract: Mothers against decapentaplegic homologue 4 (Smad4) is associated with several human cancers. However, the exact mechanism of Smad4 in human glioma is still unknown. In this study, we constructed a recombinant adenovirus encoding Smad4 and transduced it into glioma cells. The results demonstrated that the overexpression of Smad4 not only suppressed glioma cell proliferation but also increased cell apoptosis by promoting cell cycle arrest at G1 phase. Furthermore, an adenovirus encoding Smad4 suppressed tumor formation in nude mice. These findings clearly demonstrate that Smad4 plays an important role in human glioma development by regulating cell proliferation. Moreover, Smad4 may represent a potential therapeutic target in glioma.
authors: Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China.
authors: Zhao
authors: Z
authors: Yang
authors: Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China.
authors: Lina
authors: L
authors: Zhong
authors: Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China.
authors: Shanchuan
authors: S
authors: Zhong
authors: Department of Neurosurgery, the 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors: Ronghua
authors: R
authors: Xian
authors: Department of Neurosurgery, the 476th Hospital of PLA, Fuzhou, Fujian 350025, China. Electronic address: ytt83812280@sina.com.
authors: Bangqing
authors: B
authors: Yuan
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006131:Growth Inhibitors / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D051901:Smad4 Protein / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D006131:Growth Inhibitors; D051901:Smad4 Protein
keywords: Adenovirus; Glioma cell; Proliferation; Smad4