title: Evaluation of the effect of the structure of bacterial cellulose on full thickness skin wound repair on a microfluidic chip.
abstract: Bacterial cellulose (BC) is a kind of nanobiomaterial for tissue engineering. How the nanoscale structure of BC affects skin wound repair is unexplored. Here, the hierarchical structure of BC films and their different effects on skin wound healing were studied both in vitro and in vivo. The bottom side of the BC film had a larger pore size, and a looser and rougher structure than that of the top side. By using a microfluidics-based in vitro wound healing model, we revealed that the bottom side of the BC film can better promote the migration of cells to facilitate wound healing. Furthermore, the full-thickness skin wounds on Wistar rats demonstrated that, compared with gauze and the top side of the BC film, the wound covered by the bottom side of the BC film showed faster recovery rate and less inflammatory response. The results indicate that the platform based on the microfluidic chip provide a rapid, reliable, and repeatable method for wound dressing screening. As an excellent biomaterial for wound healing, the BC film displays different properties on different sides, which not only provides a method to optimize the biocompatibility of wound dressings but also paves a new way to building heterogeneous BC-based biomaterials for complex tissue engineering.
authors: National Engineering Research Center for Nano-Medicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan, 430074, China.
authors: Ying
authors: Y
authors: Li
authors: Shiwen
authors: S
authors: Wang
authors: Rong
authors: R
authors: Huang
authors: Zhuo
authors: Z
authors: Huang
authors: Binfeng
authors: B
authors: Hu
authors: Wenfu
authors: W
authors: Zheng
authors: Guang
authors: G
authors: Yang
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D000818:Animals; D001458:Bandages; D002482:Cellulose / Q000627:therapeutic use*; D004285:Dogs; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D008422:Materials Testing; D051379:Mice; D044085:Microfluidics; D041681:NIH 3T3 Cells; D011135:Polysaccharides, Bacterial / Q000627:therapeutic use*; D017208:Rats, Wistar; D012867:Skin / Q000503:physiopathology*; D014945:Wound Healing
chemical_list: D011135:Polysaccharides, Bacterial; D002482:Cellulose