title: Development of antibacterial coating on silicone surface via chlorhexidine-loaded nanospheres.
abstract: Urinary tract infections (UTIs) are the most common type of hospital-acquired infection which cause significant morbidity and mortality. Antibacterial urinary devices to prevent UTIs are in great demand, while the problem of releasing antibacterials is still limited by duration of antibacterial release and hinders their clinical applications. This study investigated a new approach to sustain release of chlorhexidine (CHX) from urinary devices by coating of chlorhexidine-loaded nanospheres (CHX-NPs) on the surface. CHX-NPs were prepared by high-pressure emulsification-solvent evaporation technique that provided the size of nanospheres at 198.8 nm and the drug loading content at 5.6%. These nanospheres were spray-coated on silicone surface with reproducible and predictable amount of CHX. Release studies conducted in artificial urine to mimic in vivo condition showed that suitable dose of CHX was released in a sustained manner within a couple of weeks. Additionally, CHX-NPs showed antibacterial activity against common bacteria causing UTIs up to 15 days, which is threefold longer than that of physical mixing between CHX and polymer. Results from this study suggest possible applications of CHX-NPs in coating the surface of ureteral-relating devices for sustained antibacterial release.
authors: Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, 25/25 Puttamonthon 4 Salaya, Nakhon Pathom, 73170, Thailand.
authors: Hathaichanok
authors: H
authors: Phuengkham
authors: Norased
authors: N
authors: Nasongkla
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002710:Chlorhexidine / Q000008:administration & dosage* / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000138:chemical synthesis; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000138:chemical synthesis; D004058:Diffusion; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D054118:Nanospheres / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D012828:Silicones / Q000737:chemistry*; D062885:Urinary Catheters / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D012828:Silicones; D002710:Chlorhexidine
references: J Mater Sci Mater Med. 2014 Jun;25(6):1471-81
references: 24510225
references: Invest Urol. 1976 Mar;13(5):346-50
references: 815197
references: Am J Infect Control. 2007 Nov;35(9):594-9
references: 17980238
references: Am J Infect Control. 2000 Feb;28(1):68-75
references: 10679141
references: Toxicol In Vitro. 2001 Aug-Oct;15(4-5):271-6
references: 11566548
references: Biomaterials. 2004 Sep;25(20):5003-12
references: 15109862
references: Science. 1999 May 21;284(5418):1318-22
references: 10334980
references: Nano Lett. 2006 Nov;6(11):2427-30
references: 17090068
references: Expert Rev Med Devices. 2009 Sep;6(5):553-67
references: 19751126
references: J Mater Sci Mater Med. 2013 Aug;24(8):1825-35
references: 23661258
references: Int Wound J. 2008 Jun;5(3):416-22
references: 18205786
references: J Control Release. 1999 Feb 22;57(3):259-68
references: 9895413
references: J Microencapsul. 2013;30(4):390-7
references: 23181625
references: Antimicrob Agents Chemother. 1994 Jul;38(7):1490-5
references: 7979277
references: J Chemother. 2006 Oct;18(5):473-9
references: 17127222
references: J Control Release. 2005 Nov 2;108(1):178-89
references: 16162366
references: J Antimicrob Chemother. 2008 Mar;61(3):651-7
references: 18258612
references: J Control Release. 2001 Jan 29;70(1-2):1-20
references: 11166403
references: Int J Pharm. 2008 Mar 20;352(1-2):317-23
references: 18160236
references: Soft Matter. 2014 Feb 14;10 (6):804-7
references: 24838052
references: Clin Microbiol Rev. 1999 Jan;12(1):147-79
references: 9880479
references: Pharm Dev Technol. 2016;21(1):8-13
references: 25220889
references: Int J Pharm. 2007 May 24;336(2):367-75
references: 17207944
references: Beilstein J Nanotechnol. 2014 Jun 18;5:872-80
references: 24991524
references: J Control Release. 2012 Jan 10;157(1):64-71
references: 21939701
references: N Engl J Med. 2013 Feb 7;368(6):533-42
references: 23388005
references: Antimicrob Agents Chemother. 1999 Dec;43(12):2990-5
references: 10582894
references: Int J Pharm. 2010 Dec 15;402(1-2):175-83
references: 20951780
references: Infect Control Hosp Epidemiol. 2009 Feb;30(2):190-2
references: 19090771
references: Orthopedics. 2012 Jul 1;35(7):e1046-50
references: 22784898
references: Clin Infect Dis. 2010 Mar 1;50(5):625-63
references: 20175247
references: Chem Pharm Bull (Tokyo). 2004 Nov;52(11):1273-9
references: 15516745