title: Mechanism of alcohol-enhanced lucigenin chemiluminescence in alkaline solution.
abstract: The chemiluminescence (CL) of lucigenin (Luc(2+)) can be enhanced by different alcohols in alkaline solution. The effect of different fatty alcohols on the CL of lucigenin was related to the carbon chain length and the number of hydroxyl groups. Glycerol provides the greatest enhancement. UV/Vis absorption spectra and fluorescence spectra showed that N-methylacridone (NMA) was produced in the CL reaction in the presence of different alcohols. The peak of the CL spectrum was located at 470 nm in all cases, indicating that the luminophore was always the excited-state NMA. The quenching of lucigenin CL by superoxide dismutase (SOD) and the electron spin resonance (ESR) results with the spin trap of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) demonstrated that superoxide anions (O2 (•-)) were generated from dissolved oxygen in the CL reaction and that glycerol and dihydroxyacetone (DHA) can promote O2 (•-) production by the reduction of dissolved oxygen in alkaline solution. It was assumed that the enhancement provided by different alcohols was related to the solvent effect and reducing capacity. Glycerol and DHA can also reduce Luc(2+) into lucigenin cation radicals (Luc(•+) ), which react with O2 (•-) to produce CL, and glycerol can slowly transform into DHA, which is oxidized quickly in alkaline solution.
authors: College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, People's Republic of China.
authors: Quan
authors: Q
authors: Chi
authors: College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, People's Republic of China.
authors: Wanying
authors: W
authors: Chen
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors: Zhike
authors: Z
authors: He
mesh_terms: D000166:Acridines / Q000737:chemistry*; D000438:Alcohols / Q000737:chemistry*; D000468:Alkalies / Q000737:chemistry*; D049449:Luminescence; D012996:Solutions
chemical_list: D000166:Acridines; D000438:Alcohols; D000468:Alkalies; D012996:Solutions; C033472:10,10'-dimethyl-9,9'-biacridinium
keywords: chemiluminescence; dihydroxyacetone; glycerol; lucigenin; superoxide anion