title: Clinical-scale in vitro expansion preserves biological characteristics of cardiac atrial appendage stem cells.
abstract: OBJECTIVE
  Cardiac atrial appendage stem cells (CASCs) have recently emerged as an attractive candidate for cardiac regeneration after myocardial infarction. As with other cardiac stem cells, CASCs have to be expanded ex vivo to obtain clinically relevant cell numbers. However, foetal calf serum (FCS), which is routinely used for cell culturing, is unsuitable for clinical purposes, and influence of long-term in vitro culture on CASC behaviour is unknown.
  
  
  METHODS
  We examined effects on CASC biology of prolonged expansion, and evaluated a culture protocol suitable for human use.
  
  
  RESULTS
  In FCS-supplemented medium, CASCs could be kept in culture for 55.75 ± 3.63 days, before reaching senescence. Despite a small reduction in numbers of proliferating CASCs (1.37 ± 0.52% per passage) and signs of progressive telomere shortening (0.04 ± 0.02 kb per passage), their immunophenotype and myocardial differentiation potential remained unaffected during the entire culture period. The cells were successfully expanded in human platelet plasma supernatant, while maintaining their biological properties.
  
  
  CONCLUSIONS
  We successfully developed a protocol for long-term culture, to obtain clinically relevant CASC numbers, while retaining their cardiogenic potential. These insights in CASC biology and optimization of a humanized platelet-based culture method are an important step towards clinical application of CASCs for cardiac regenerative medicine.
authors: Laboratory of Experimental Hematology, Jessa Hospital, 3500, Hasselt, Belgium; Faculty of Medicine and Life Sciences, Hasselt University, 3590, Diepenbeek, Belgium.
authors: S
authors: S
authors: Windmolders
authors: L
authors: L
authors: Willems
authors: A
authors: A
authors: Daniëls
authors: L
authors: L
authors: Linsen
authors: Y
authors: Y
authors: Fanton
authors: M
authors: M
authors: Hendrikx
authors: R
authors: R
authors: Koninckx
authors: J-L
authors: JL
authors: Rummens
authors: K
authors: K
authors: Hensen
mesh_terms: D000368:Aged; D020517:Atrial Appendage / Q000166:cytology*; D001792:Blood Platelets / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D009203:Myocardial Infarction / Q000628:therapy; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*; D019098:Telomerase / Q000032:analysis; D059506:Telomere Shortening; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D019098:Telomerase
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