title: Horseradish peroxidase and antibody labeled gold nanoparticle probe for amplified immunoassay of ciguatoxin in fish samples based on capillary electrophoresis with electrochemical detection.
abstract: This paper describes a new amplified immunoassay with horseradish peroxidase (HRP) and antibody (Ab) labeled gold nanoparticles (AuNPs) probe hyphenated to capillary electrophoresis (CE) with electrochemical (EC) detection for ultrasensitive determination of ciguatoxin CTX1B. AuNPs were conjugated with HRP and Ab, and then incubated with limited amount of CTX1B to produce immunocomplex. The immunoreactive sample was injected into capillary for CE separation and EC detection. Enhanced sensitivity was obtained by adopting the AuNPs as carriers of HRP and Ab at high HRP/Ab molar ratio. The calibration curve of CTX1B was in the range of 0.06-90 ng/mL. The detection limit was 0.045 ng/mL, which is 38-fold lower than that of HPLC-MS method for CTX1B analysis. The proposed method was successfully applied for the quantification of CTX1B in contamined fish samples by simultaneously labeling Ab and HRP on AuNPs. The amplified IA with HRP and Ab labeled AuNPs probe hyphenated to CE and EC detection provides a sensitive analytical approach for the determination of trace ciguatoxin in complex samples.
authors: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address: qustzhzhx@126.com.
authors: Zhaoxiang
authors: Z
authors: Zhang
authors: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors: Ying
authors: Y
authors: Liu
authors: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors: Chaoying
authors: C
authors: Zhang
authors: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors: Wenxiu
authors: W
authors: Luan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002681:China; D002922:Ciguatoxins / Q000032:analysis*; D055664:Electrochemical Techniques; D019075:Electrophoresis, Capillary; D005399:Fishes / Q000378:metabolism*; D006046:Gold / Q000378:metabolism; D006735:Horseradish Peroxidase; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000906:Antibodies; D002922:Ciguatoxins; D006046:Gold; D006735:Horseradish Peroxidase
keywords: CTX1B; Capillary electrophoresis; Electrochemical detection; Gold nanoparticles; Immunoassay