title: Emergence of oxacillin-resistant Staphylococcus lugdunensis carrying staphylococcal cassette chromosome mec type V in central Taiwan.
abstract: BACKGROUND
  Staphylococcus lugdunensis has emerged as a key pathogen for clinical infection. It is sensitive to most antistaphylococcal agents, but it is increasingly resistant to β-lactam antibiotics. Oxacillin-resistant S. lugdunensis isolates carrying the mecA gene pose a major concern for therapy failure.
  
  
  METHODS
  To assess the epidemiology and presence of mecA in S. lugdunensis, we gauged the prevalence and antibiotic resistance of S. lugdunensis in clinical specimens by using multiplex polymerase chain reaction (PCR) and pulsed-field gel electrophoresis.
  
  
  RESULTS
  Thirty S. lugdunensis isolates were collected and examined between October 2009 and December 2010. The resistance to penicillin (87%) and oxacillin (20%) was noted. All oxacillin-resistant isolates (6/30) had type V or VTSCCmec. Most (67%, 4/6) isolates carried SCCmec type V. These organisms caused invasive infections such as peritonitis, osteomyelitis, and septic arthritis. Pulsed-field gel electrophoresis analyses showed most (83%, 5/6) isolates carrying mecA were pulsotype D with high similarity (93.8%).
  
  
  CONCLUSIONS
  The findings suggest oxacillin-resistant S. lugdunensis carrying SCCmec type V is emerging in central Taiwan.
authors: Department of Pediatrics, Children's Hospital, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan.
authors: Ting-Yu
authors: TY
authors: Yen
authors: Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
authors: Yun-Ju
authors: YJ
authors: Sung
authors: Department of Pediatrics, Children's Hospital, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan.
authors: Hsiao-Chuan
authors: HC
authors: Lin
authors: Department of Pediatrics, Children's Hospital, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan.
authors: Ching-Tien
authors: CT
authors: Peng
authors: Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
authors: Ni
authors: N
authors: Tien
authors: Department of Pediatrics, Children's Hospital, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan.
authors: Kao-Pin
authors: KP
authors: Hwang
authors: Department of Medical Research, China Medical University Hospital, Taichung, Taiwan; Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kwei-Shan, Taoyuan, Taiwan. Electronic address: janglu45@gmail.com.
authors: Jang-Jih
authors: JJ
authors: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001170:Arthritis, Infectious / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D010019:Osteomyelitis / Q000382:microbiology; D010068:Oxacillin / Q000494:pharmacology*; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D010538:Peritonitis / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology*; D057787:Staphylococcus lugdunensis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013624:Taiwan; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D010068:Oxacillin
keywords: SCCmec gene; Staphylococcus lugdunensis; coagulase-negative staphylococcus; oxacillin resistance