title: Nanoscale imaging of caveolin-1 membrane domains in vivo.
abstract: Light microscopy enables noninvasive imaging of fluorescent species in biological specimens, but resolution is generally limited by diffraction to ~200-250 nm. Many biological processes occur on smaller length scales, highlighting the importance of techniques that can image below the diffraction limit and provide valuable single-molecule information. In recent years, imaging techniques have been developed which can achieve resolution below the diffraction limit. Utilizing one such technique, fluorescence photoactivation localization microscopy (FPALM), we demonstrated its ability to construct super-resolution images from single molecules in a living zebrafish embryo, expanding the realm of previous super-resolution imaging to a living vertebrate organism. We imaged caveolin-1 in vivo, in living zebrafish embryos. Our results demonstrate the successful image acquisition of super-resolution images in a living vertebrate organism, opening several opportunities to answer more dynamic biological questions in vivo at the previously inaccessible nanoscale.
authors: Department of Physics and Astronomy, University of Maine, Orono, Maine, United States of America; Graduate School of Biomedical Sciences, University of Maine, Orono, Maine, United States of America; Department of Molecular and Biomedical Sciences, University of Maine, Orono, Maine, United States of America.
authors: Kristin A
authors: KA
authors: Gabor
authors: Department of Physics and Astronomy, University of Maine, Orono, Maine, United States of America.
authors: Dahan
authors: D
authors: Kim
authors: Graduate School of Biomedical Sciences, University of Maine, Orono, Maine, United States of America; Department of Molecular and Biomedical Sciences, University of Maine, Orono, Maine, United States of America.
authors: Carol H
authors: CH
authors: Kim
authors: Department of Physics and Astronomy, University of Maine, Orono, Maine, United States of America; Department of Molecular and Biomedical Sciences, University of Maine, Orono, Maine, United States of America.
authors: Samuel T
authors: ST
authors: Hess
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015027:Zebrafish
chemical_list: D051242:Caveolin 1
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