title: Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin.
abstract: Fast neural conduction requires accumulation of Na(+) channels at nodes of Ranvier. Dedicated adhesion molecules on myelinating cells and axons govern node organization. Among those, specific laminins and dystroglycan complexes contribute to Na(+) channel clustering at peripheral nodes by unknown mechanisms. We show that in addition to facing the basal lamina, dystroglycan is found near the nodal matrix around axons, binds matrix components, and participates in initial events of nodogenesis. We identify the dystroglycan-ligand perlecan as a novel nodal component and show that dystroglycan is required for the selective accumulation of perlecan at nodes. Perlecan binds the clustering molecule gliomedin and enhances clustering of node of Ranvier components. These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier. Further, our data indicate that dystroglycan binds free matrix that is not organized in a basal lamina.
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.
authors: Cristina
authors: C
authors: Colombelli
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203 Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203.
authors: Marilena
authors: M
authors: Palmisano
authors: Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Yael
authors: Y
authors: Eshed-Eisenbach
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.
authors: Desirée
authors: D
authors: Zambroni
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.
authors: Ernesto
authors: E
authors: Pavoni
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.
authors: Cinzia
authors: C
authors: Ferri
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy.
authors: Stefania
authors: S
authors: Saccucci
authors: Institut du Cerveau et de la Moelle Épinière, 75013 Paris, France Institut National de la Santé et de la Recherche Médicale, U1127, 75019 Paris, France Sorbonne Universités, Université Pierre et Marie Currie, UMRS1127, 75252 Paris, France Centre National de la Recherche Scientifique, UMR 7225, 75013 Paris, France.
authors: Sophie
authors: S
authors: Nicole
authors: Oulu Center for Cell-Extracellular Matrix Research, University of Oulu, 90014 Oulu, Finland.
authors: Raija
authors: R
authors: Soininen
authors: Rutgers University, Piscataway, NJ 08854.
authors: Karen K
authors: KK
authors: McKee
authors: Rutgers University, Piscataway, NJ 08854.
authors: Peter D
authors: PD
authors: Yurchenco
authors: Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Elior
authors: E
authors: Peles
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203 Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203.
authors: Lawrence
authors: L
authors: Wrabetz
authors: Division of Genetics and Cell Biology, San Raffaele Hospital, 20132 Milan, Italy Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203 Department of Biochemistry and Department of Neurology, Hunter James Kelly Research Institute, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14203 mlfeltri@buffalo.edu.
authors: M Laura
authors: ML
authors: Feltri
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002478:Cells, Cultured; D018920:Coculture Techniques; D049030:Dystroglycans / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006801:Humans; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008871:Microvilli / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D059748:Proteolysis; D011901:Ranvier's Nodes / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D019812:Heparan Sulfate Proteoglycans; D015222:Sodium Channels; C520020:gliomedin protein, mouse; C071927:perlecan; D049030:Dystroglycans
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