title: Krt6a-positive mammary epithelial progenitors are not at increased vulnerability to tumorigenesis initiated by ErbB2.
abstract: While most breast cancers are thought to arise from the luminal layer of the breast tissue, it remains unclear which specific cells in the luminal layer are the cells of origin of breast cancer. We have previously reported that WAP-positive luminal epithelial cells are at increased susceptibility to tumor initiation by ErbB2 compared to the bulk population, while the mammary cells with canonical Wnt signaling activity fail to evolve into tumors upon ErbB2 activation. Here, we used retrovirus to introduce ErbB2 into the Krt6a-positive mammary progenitor subset of the luminal epithelium and, for comparison, into the mammary luminal epithelium indiscriminately. Tumors developed from both groups of cells with a similar latency. These data indicate that the Krt6a-positive subset of mammary epithelial cells can be induced to form cancer by ErbB2 but it is not more susceptible to tumorigenesis initiated by ErbB2 than the bulk population of the luminal epithelium.
authors: Lester & Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, United States of America.
authors: Kimberly R
authors: KR
authors: Holloway
authors: Lester & Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, United States of America; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, United States of America.
authors: Vidya C
authors: VC
authors: Sinha
authors: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, United States of America.
authors: Michael J
authors: MJ
authors: Toneff
authors: Lester & Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, United States of America; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, United States of America.
authors: Wen
authors: W
authors: Bu
authors: Lester & Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, United States of America.
authors: Susan G
authors: SG
authors: Hilsenbeck
authors: Lester & Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, United States of America; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, United States of America; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America.
authors: Yi
authors: Y
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D053553:Keratin-6 / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000473:pathology*; D008324:Mammary Tumor Virus, Mouse / Q000378:metabolism; D008822:Mice, Transgenic; D010766:Phosphorylation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D012190:Retroviridae / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D014771:Virion / Q000378:metabolism; D017735:Virus Latency
chemical_list: D053553:Keratin-6; D018719:Erb-b2 Receptor Tyrosine Kinases; D050799:STAT5 Transcription Factor; C524056:Erbb2 protein, mouse
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