title: [Mechanism study on leptin resistance in lung cancer cachexia rats treated by Xiaoyan Decoction].
abstract: OBJECTIVE
  To study the leptin resistance mechanism of Xiaoyan Decoction (XD) in lung cancer cachexia (LCC) rats.
  
  
  METHODS
  An LCC rat model was established. Totally 40 rats were randomly divided into the normal control group, the LCC model group, the XD group, and the positive control group, 10 in each group. After LCC model was set up, rats in the LCC model group were administered with normal saline, 2 mL each time. Rats in the XD group were administered with XD at the daily dose of 2 mL. Those in the positive control group were administered with Medroxyprogesterone Acetate suspension (20 mg/kg) by gastrogavage at the daily dose of 2 mL. All medication lasted for 14 days. The general condition and tumor growth were observed. Serum levels of leptin and leptin receptor in the hypothalamus were detected using enzyme-linked immunosorbent assay. Contents of neuropeptide Y (NPY) and anorexia for genomic POMC were detected using real-time PCR technique.
  
  
  RESULTS
  Serum leptin levels were lower in the LCC model group than in the normal control group with statistical significance (P < 0.05). Compared with the LCC model groups, serum leptin levels significantly increased in the XD group (P < 0.01). Leptin receptor levels in the hypothalamus increased significantly in the LCC model group (P < 0.01). Increased receptor levels in the LCC model group indicated that either XD or Medroxyprogesterone Acetate could effectively reduce levels of leptin receptor with statistical significance (P < 0.01). There was also statistical difference between the XD group and the positive control group (P < 0.05). Contents of NPY was higher in the LCC model group than in the other groups with statistical difference (P < 0.05). There was no statistical difference in NPY between the normal control group and the rest 2 treatment groups (P > 0.05). There was statistical difference in POMC between the normal control group and the LCC model group (P < 0.05). POMC could be decreased in the XD group and the positive control group with statistical significance (P < 0.05), and it was more obviously decreased in the XD group (P < 0.05).
  
  
  CONCLUSIONS
  Leptin resistance existed in LCC rats. XD could increase serum leptin levels and reduce leptin receptor levels in the hypothalamus. LCC could be improved by elevating NPY contents in the hypothalamus and reducing POMC contents, promoting the appetite, and increasing food intake from the periphery pathway and the central pathway.
authors: Yun-Chao
authors: YC
authors: Zhang
authors: Ying-Jie
authors: YJ
authors: Jia
authors: Pei-Ying
authors: PY
authors: Yang
authors: Xing
authors: X
authors: Zhang
authors: Xiao-Jiang
authors: XJ
authors: Li
authors: Ying
authors: Y
authors: Zhang
authors: Jin-Li
authors: JL
authors: Zhu
authors: Yi-Yu
authors: YY
authors: Sun
authors: Jun
authors: J
authors: Chen
authors: Hao-Guo
authors: HG
authors: Duan
authors: Hua
authors: H
authors: Guo
authors: Chao
authors: C
authors: Li
mesh_terms: D000818:Animals; D002100:Cachexia / Q000188:drug therapy* / Q000209:etiology; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004435:Eating; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008175:Lung Neoplasms / Q000150:complications*; D009478:Neuropeptide Y / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D020738:Leptin; D009478:Neuropeptide Y