title: Expression and role of V1A subunit of V-ATPases in gastric cancer cells.
abstract: BACKGROUND
  Vacuolar-ATPases (V-ATPases) play an important role in maintaining a relatively neutral pHi (internal pH) and are responsible for the progression of cancer. V-ATPases contain different subunits and few studies have been conducted on subunit V1A. This study aimed to investigate the gene expression of V1A subunit of V-ATPases in gastric cancer tissues and explore its role in the progression and prognosis of gastric cancer.
  
  
  METHODS
  The protein expressions of the V1A subunit of V-ATPase gene in 100 normal gastric specimens and 100 gastric cancer tissues were determined by immunohistochemistry. The role of V1A subunit of V-ATPases was studied using a specific small interfering RNA (siRNA).
  
  
  RESULTS
  The positive expression rate of the V1A subunit of V-ATPases was 76 % in gastric cancer tissue samples, much higher than that in normal tissue samples (30 %, P < 0.05), and was correlated with histological grade (P = 0.001), lymph node metastasis (P = 0.002), TNM (P = 0.040), and vascular invasion (P = 0.010), but not with patient age, sex, depth of tumor invasion, tumor size, or histological type. The median overall survival times of 76 patients who had positive staining for tumor cell V1A subunit of V-ATPases and 24 patients who had negative staining were 31.7 and 59.2 months, respectively. When the expression of V1A subunit was knocked down using siRNA, the proliferation and invasion of gastric cancer cells in vitro were significantly inhibited.
  
  
  CONCLUSIONS
  V1A subunit of V-ATPases can be a prognostic indicator for poor outcome and is a therapeutic target in gastric cancer.
authors: Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, Wuxi, 214400, People's Republic of China.
authors: Pengfei
authors: P
authors: Liu
authors: Haijiao
authors: H
authors: Chen
authors: Lanxiu
authors: L
authors: Han
authors: Xiaoping
authors: X
authors: Zou
authors: Weidong
authors: W
authors: Shen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018450:Disease Progression; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D021122:Protein Subunits / Q000096:biosynthesis* / Q000235:genetics; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D034741:RNA, Small Interfering; D025262:Vacuolar Proton-Translocating ATPases
references: Future Oncol. 2005 Dec;1(6):779-86
references: 16556057
references: Cell Biochem Biophys. 2004;40(2):185-206
references: 15054222
references: BMC Biochem. 2005 Aug 04;6:13
references: 16080788
references: Cancer Res. 2005 Aug 1;65(15):6843-9
references: 16061667
references: Cancer Treat Rev. 2009 Dec;35(8):707-13
references: 19758758
references: Braz J Biol. 2012 Feb;72(1):189-98
references: 22437401
references: FEBS Lett. 2006 May 15;580(11):2723-30
references: 16647709
references: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references: 15761078
references: Biotech Histochem. 2012 Feb;87(2):133-9
references: 21526910
references: Pflugers Arch. 2009 Jan;457(3):589-98
references: 18026982
references: J Exp Biol. 2006 Feb;209(Pt 4):577-89
references: 16449553
references: Cancer Sci. 2009 Aug;100(8):1460-7
references: 19459857
references: Cell Biol Int. 2009 Sep;33(9):1008-19
references: 19501661
references: Mol Pharmacol. 2006 Dec;70(6):1856-65
references: 16940187
references: Pigment Cell Melanoma Res. 2013 Mar;26(2):205-17
references: 23205854
references: Dis Model Mech. 2013 May;6(3):689-700
references: 23335205
references: Nat Cell Biol. 2006 Feb;8(2):124-36
references: 16415858
references: Cancer Res. 2007 Jun 1;67(11):5408-17
references: 17545622
references: Neuron. 2010 Dec 22;68(6):1020-2
references: 21172605
references: Pathology. 2001 Aug;33(3):271-7
references: 11523923
references: Am J Physiol Cell Physiol. 2004 Jun;286(6):C1443-52
references: 14761893
references: Cancer Cell. 2004 Feb;5(2):121-5
references: 14998488
references: Cancer Res. 2012 Aug 15;72(16):3938-47
references: 22719070
references: J Clin Gastroenterol. 2003 May-Jun;36(5 Suppl):S29-36; discussion S61-2
references: 12702963