title: Effects of kallistatin on oxidative stress and inflammation on renal ischemia-reperfusion injury in mice.
abstract: BACKGROUND
  Kallistatin (KS) is a serine proteinase. The result of KS on 'Renal Ischemia- Reperfusion Injury' (IRI) has not clearly been researched. In this study, investigative research has been conducted to draw results on the administration of human KS on kidney response conducted within a mouse model of IRI.
  
  
  METHODS
  BALB/c mice were used and given 30 min ischemia that was injected into the kidney which was followed with 24 h reperfusion. The human KS gene contained within an adenoviral vector was injected intravenously 30 min before reperfusion and 12h after reperfusion. Analyses illustrated what impact KS had on renal IRI in realtion to tubular necrosis, apoptotic cell death, inflammatory cytokines, renal function, and inflammatory cell infiltration.
  
  
  RESULTS
  KS gene transfer significantly had a positive impact on renal function (reduced blood urea-nitrogen: 73.5±13.6 vs. 195.4±14.6 mg/dL at day three, p < 0.05 and the serum creatinine levels: 0.23±0.02 vs. 0.71±0.14 mg/dL at day three, p < 0.05), reduced tubular necrosis and apoptosis of IRI kidneys. The permeation of cells that were inflamed and the manufacturing of pro-inflammatory cytokines (RANTES-is regulated through been activated with normal T-cell which are expressed and secreted, tumour necrosis interleukin-1β factor-α, and interferon-γ) resulted in significantly suppressing KS in mice with IRI. The efficacy to scavenge superoxide in tubule cells was also demonstrated by high-performance liquid chromatography.
  
  
  CONCLUSIONS
  Our study suggests a novel role of KS in renal protection after 'Renal Ischemia-Reperfusion Injury' blocking of oxidative stress and renal inflammation.
authors: Shuqin
authors: S
authors: Zhou
authors: Yingying
authors: Y
authors: Sun
authors: Yugang
authors: Y
authors: Zhuang
authors: Wei
authors: W
authors: Zhao
authors: Yuanzhuo
authors: Y
authors: Chen
authors: Bojie
authors: B
authors: Jiang
authors: Changfeng
authors: C
authors: Guo
authors: Zhonglin
authors: Z
authors: Zhang
authors: Department of Emergency & Critical Care Medicine，Shanghai Tenth People's Hospital, Tongji University, 301 Yanchang Road, Shanghai 200072, PR China. penghu_shdsyy@126.com.
authors: Hu
authors: H
authors: Peng
authors: Yanqing
authors: Y
authors: Chen
mesh_terms: D058186:Acute Kidney Injury / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D009336:Necrosis; D009393:Nephritis / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D018384:Oxidative Stress; D015427:Reperfusion Injury / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D015843:Serpins; C051324:kallistatin; D003404:Creatinine