title: Comparison of eight commercially available kits for DNA extraction from formalin-fixed paraffin-embedded tissues.
abstract: A proper extraction method from formalin-fixed paraffin-embedded (FFPE) blocks is essential to obtain DNA of satisfactory quality/quantity. We compared the effectiveness of eight commercially available kits for DNA extraction based on 10 FFPE tissues. Kits differed significantly in terms of DNA yield, purity, and quality. Using the QIAamp DNA FFPE Tissue Kit (Qiagen) and the ReliaPrep FFPE gDNA Miniprep System (Promega), we obtained DNA of the highest quality and acceptable quantity. We also demonstrated that overnight digestion of samples usually improved DNA yield and/or purity. For precious or limited material, double elution is recommended for obtaining up to 42% higher amount of DNA.
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Centre and Institute of Oncology, Krakow Branch, 31-115 Krakow, Poland. Electronic address: janecka.zrk@gmail.com.
authors: Anna
authors: A
authors: Janecka
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Centre and Institute of Oncology, Krakow Branch, 31-115 Krakow, Poland.
authors: Agnieszka
authors: A
authors: Adamczyk
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Centre and Institute of Oncology, Krakow Branch, 31-115 Krakow, Poland.
authors: Anna
authors: A
authors: Gasińska
mesh_terms: D004247:DNA / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry*; D005821:Genetic Techniques / Q000592:standards*; D016612:Paraffin Embedding; D011933:Reagent Kits, Diagnostic; D016707:Tissue Fixation
chemical_list: D011933:Reagent Kits, Diagnostic; D005557:Formaldehyde; D004247:DNA
keywords: Commercially available kit; DNA extraction; DNA yield and purity; FFPE tissue; Real-time PCR