title: CE-MS-based proteomics in biomarker discovery and clinical application.
abstract: CE-MS is applied in clinical proteomics for both the identification of biomarkers of disease and assessment of biomarkers in clinical diagnosis. The analysis is reproducible, fast, and requires only small sample volumes. However, successful CE-MS analysis depends on several critical steps that can be consolidated as follows: (i) proper sample preparation and fractionation, (ii) application of suitable capillary coating and appropriate CE-MS interfaces, to ensure the reproducibility and stability of the analysis, and (iii) an optimized clinical and statistical study design to increase the chances for obtaining clinically relevant results. In this review, we cover all these aspects, and present several examples of the application of CE-MS in clinical proteomics.
authors: Department of R&D, Mosaiques Diagnostics GmbH, Hanover, Germany; Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: Claudia
authors: C
authors: Pontillo
authors: Szymon
authors: S
authors: Filip
authors: Daniel M
authors: DM
authors: Borràs
authors: William
authors: W
authors: Mullen
authors: Antonia
authors: A
authors: Vlahou
authors: Harald
authors: H
authors: Mischak
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers
keywords: Biomarker; Capillary electrophoresis; Clinical proteomics; Mass spectrometry; Peptidomics