title: Lateralization, maturation, and anteroposterior topography in the lateral habenula revealed by ZIF268/EGR1 immunoreactivity and labeling history of neuronal activity.
abstract: We report habenular lateralization in a simple transgenic mouse model used for labeling a facet of neuronal activity history. A transgenic construct comprised of a zif268/egr1 immediate-early gene promoter and a gene for normal Venus fluorescent protein with a membrane tag converted promoter activity into long-life fluorescent proteins, which was thought to describe a facet of neuronal activity history by summing neuronal activity. In addition to mapping the immediate-early gene-immunopositive cells, this method helped demonstrate the functionality of the lateral habenular nucleus (LHb). During postnatal development, the LHb was activated between postnatal days 10 and 16. The water-immersion restraint stress also activated the LHb over a similar period. LHb activation was functionally lateralized, but had no directional bias at the population level. Moreover, the posterior LHb was activated in the early stage after the stress, while the anterior LHb was activated in the later stage. Our results indicate lateralization, maturation, and anteroposterior topography of the LHb during postnatal development and the stress response.
authors: Department of Anatomy, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan; Department of Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan. Electronic address: ichijo@med.u-toyama.ac.jp.
authors: Hiroyuki
authors: H
authors: Ichijo
authors: Department of Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors: Michito
authors: M
authors: Hamada
authors: Department of Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan; Laboratory Animal Resource Center, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors: Satoru
authors: S
authors: Takahashi
authors: Department of Molecular and Developmental Biology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors: Makoto
authors: M
authors: Kobayashi
authors: The Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567-0047, Japan.
authors: Takeharu
authors: T
authors: Nagai
authors: Department of Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors: Tomoko
authors: T
authors: Toyama
authors: Department of Anatomy, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors: Masahumi
authors: M
authors: Kawaguchi
mesh_terms: D000818:Animals; D051766:Early Growth Response Protein 1 / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D007839:Functional Laterality; D019262:Habenula / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012149:Restraint, Physical; D013194:Staining and Labeling / Q000379:methods*; D013315:Stress, Psychological / Q000378:metabolism
chemical_list: D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D008164:Luminescent Proteins
keywords: Asymmetry; Fasciculus retroflexus; Habenula; Immediate-early gene; Stress