title: DNA barcoding of Japanese click beetles (Coleoptera, Elateridae).
abstract: Click beetles (Coleoptera: Elateridae) represent one of the largest groups of beetle insects. Some click beetles in larval form, known as wireworms, are destructive agricultural pests. Morphological identification of click beetles is generally difficult and requires taxonomic expertise. This study reports on the DNA barcoding of Japanese click beetles to enable their rapid and accurate identification. We collected and assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% of the common species found on the Japanese main island, Honshu. This barcode library also contains 20 out of the 21 potential pest species recorded in Japan. Our analysis shows that most morphologically identified species form distinct phylogenetic clusters separated from each other by large molecular distances. This supports the general usefulness of the DNA barcoding approach for quick and reliable identification of Japanese elaterid species for environmental impact assessment, agricultural pest control, and biodiversity analysis. On the other hand, the taxonomic boundary in dozens of species did not agree with the boundary of barcode index numbers (a criterion for sequence-based species delimitation). These findings urge taxonomic reinvestigation of these mismatched taxa.
authors: Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
authors: Yuichi
authors: Y
authors: Oba
authors: Kitsuneyama, Maigi-cho, Okazaki, 444-3511, Japan.
authors: Hitoo
authors: H
authors: Ôhira
authors: Research Center for Biological Diversity, Graduate School of Natural Sciences, Nagoya City University, Nagoya, 467-8501, Japan.
authors: Yukio
authors: Y
authors: Murase
authors: Research Center for Biological Diversity, Graduate School of Natural Sciences, Nagoya City University, Nagoya, 467-8501, Japan.
authors: Akihiko
authors: A
authors: Moriyama
authors: Research Center for Biological Diversity, Graduate School of Natural Sciences, Nagoya City University, Nagoya, 467-8501, Japan.
authors: Yoshinori
authors: Y
authors: Kumazawa
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D003576:Electron Transport Complex IV / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics; D007564:Japan; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV
references: Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
references: 7881515
references: J Chem Ecol. 2007 Feb;33(2):345-51
references: 17191156
references: Mol Ecol Notes. 2007 May 1;7(3):355-364
references: 18784790
references: PLoS One. 2013;8(7):e66213
references: 23861743
references: Bull Entomol Res. 2011 Apr;101(2):201-10
references: 21034519
references: J Econ Entomol. 2008 Oct;101(5):1568-74
references: 18950038
references: Proc Biol Sci. 2003 Aug 7;270 Suppl 1:S96-9
references: 12952648
references: Mol Phylogenet Evol. 2007 Feb;42(2):410-21
references: 16971143
references: PLoS One. 2014;9(9):e108651
references: 25255319