title: Rapid simultaneous determination of eperisone, tolperisone, and tizanidine in human serum by using a MonoSpin® C18 extraction column and liquid chromatography/tandem mass spectrometry.
abstract: A method was developed for rapid toxicological analysis of eperisone, tolperisone, and tizanidine in human serum using a MonoSpin® C18 extraction column and LC/MS/MS. The method was validated for LOD, linearity, precision, and extraction recovery. This method was rapid with an LOD of 0.5 ng/mL, linearity range 1-500.0 ng/mL (r2 = 0.999), and RSD value below 14.6%. Extraction recovery from the sample was greater than 98.6, 98.8, and 88.5% for eperisone, tolperisone, and tizanidine, respectively. Results showed that combination of the MonoSpin C18 extraction column and LC/MS/MS is a simple and rapid method for the analysis of these three analytes, and a method is described for simultaneous quantitative determination of the analytes in human serum by LC/MSIMS. This method was used to determine the serum levels of eperisone in a patient with eperisone poisoning, and could be successfully applied for screening analyses in clinical cases other than poisoning.
authors: Naoya
authors: N
authors: Miura
authors: Takeshi
authors: T
authors: Saito
authors: Takayuki
authors: T
authors: Taira
authors: Takeshi
authors: T
authors: Yamagiwa
authors: Sein
authors: S
authors: Morita
authors: Sadaki
authors: S
authors: Inokuchi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D003000:Clonidine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008875:Middle Aged; D009125:Muscle Relaxants, Central / Q000097:blood*; D011427:Propiophenones / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D014049:Tolperisone / Q000097:blood*
chemical_list: D009125:Muscle Relaxants, Central; D011427:Propiophenones; C030848:eperisone; C023754:tizanidine; D014049:Tolperisone; D003000:Clonidine