title: Dissecting molecular aspects of cell interactions using glycodendrimersomes with programmable glycan presentation and engineered human lectins.
abstract: Glycodendrimersomes with programmable surface display of glycan, together with artificially engineered galectins, were used to understand the physiological significance of human lectins with homodimeric and tandem-repeat-type displays. The mode of topological surface presentation and the density of glycan affected vesicle aggregation mediated by multivalent carbohydrate-protein interactions. The cross-linking capacity of homodimeric lectins was enhanced by covalent connection of the two carbohydrate-binding sites. These findings highlight the value of glycodendrimersomes as versatile cell membrane mimetics, and assays provide diagnostic tools for protein functionality. This work also provides guidelines for the design of cell separators, bioactive matrices, bioeffectors, and other biomedical applications.
authors: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323 (USA) http://percec02.chem.upenn.edu/
authors: Shaodong
authors: S
authors: Zhang
authors: Ralph-Olivier
authors: RO
authors: Moussodia
authors: Claire
authors: C
authors: Murzeau
authors: Hao-Jan
authors: HJ
authors: Sun
authors: Michael L
authors: ML
authors: Klein
authors: Sabine
authors: S
authors: Vértesy
authors: Sabine
authors: S
authors: André
authors: René
authors: R
authors: Roy
authors: Hans-Joachim
authors: HJ
authors: Gabius
authors: Virgil
authors: V
authors: Percec
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000737:chemistry; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D002477:Cells / Q000187:drug effects*; D003208:Concanavalin A / Q000737:chemistry; D003432:Cross-Linking Reagents; D050091:Dendrimers / Q000737:chemistry*; D005456:Fluorescent Dyes; D037161:Galectins / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D013499:Surface Properties; D020080:Tandem Repeat Sequences
chemical_list: D002241:Carbohydrates; D003432:Cross-Linking Reagents; D050091:Dendrimers; D005456:Fluorescent Dyes; D037161:Galectins; D003208:Concanavalin A
keywords: adhesion; agglutination; dendrimers; lectin; vesicles