title: Sequencing of the mitochondrial genome of the avocado lace bug Pseudacysta perseae (Heteroptera, Tingidae) using a genome skimming approach.
abstract: Lace bugs (Tingidae) are a family of phytophagous heteropterans, some of which are important agricultural and forestry pests. They currently comprise around 2500 species distributed worldwide, for which only one mitochondrial genome has been described so far. We sequenced the complete mitochondrial genome and the nuclear ribosomal gene segment of the avocado lace bug Pseudacysta perseae using a genome skimming approach on an Illumina Hiseq 2000 platform. Fifty-four additional heteropteran mitogenomes, including the one of the sycamore lace bug Corythucha ciliata, were retrieved to allow for comparisons and phylogenetic analyses. P. perseae mitochondrial genome was determined to be 15,850 bp long, and presented the typical organisation of insect mitogenomes. The phylogenetic analysis placed P. perseae as a sister to C. ciliata but did not confirm the monophyly of Miroidae including Tingidae. Our results contradicted widely accepted phylogenetic hypothesis, which highlights the limits of analyses based on mitochondrial data only. Shotgun sequencing approaches should provide substantial improvements in harmonizing mitochondrial and nuclear databases.
authors: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), université Paul-Sabatier, bâtiment 4R1, 118, route de Narbonne, 31062 Toulouse cedex 9, France. Electronic address: arthur.kocher@gmail.com.
authors: Arthur
authors: A
authors: Kocher
authors: UMR 7205 CNRS, CP50, département de systématique et évolution, Muséum national d'histoire naturelle, 57, rue Cuvier, 75005 Paris, France.
authors: Éric
authors: É
authors: Guilbert
authors: INRA, UAR 1209 département de génétique animale, GeT-PlaGe, Campus INRA, Auzeville, 31326 Castanet-Tolosan, France.
authors: Émeline
authors: É
authors: Lhuillier
authors: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), université Paul-Sabatier, bâtiment 4R1, 118, route de Narbonne, 31062 Toulouse cedex 9, France.
authors: Jerôme
authors: J
authors: Murienne
mesh_terms: D000818:Animals; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D020062:Heteroptera / Q000235:genetics*; D027421:Persea; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
keywords: French Guiana; Guyane; Illumina Hiseq; Mitogenome; Mitogénome; Phylogenomics; Phylogénomique; Shotgun sequencing; Séquençage aléatoire