title: Structural, functional, and ACE inhibitory properties of water-soluble polysaccharides from chickpea flours.
abstract: The present study aimed to characterize and investigate the functional and angiotensin-I converting enzyme (ACE) inhibition activities of chickpea water-soluble polysaccharides (CPWSP). Physico-chemical characteristics were determined by nuclear magnetic resonance spectroscopy (NMR), Fourier transform-infrared spectroscopy (FT-IR) analysis, and X-ray diffractometry (XRD). Functional properties (water holding capacity: WHC, water solubility index: WSI, swelling capacity: SC, oil holding capacity: OHC, foaming, and emulsion properties) and ACE activities were also investigated using well-established procedures. The FT-IR spectra obtained for the CPWSP revealed two significant peaks, at about 3500 and 500 cm(-1), which corresponded to the carbohydrate region and were characteristic of polysaccharides. All spectra showed the presence of a broad absorption between 1500 and 670 cm(-1), which could be attributed to CH, CO, and OH bands in the polysaccharides. CPWSP had an XRD pattern that was typical for a semi-crystalline polymer with a major crystalline reflection at 19.6 °C. They also displayed important techno-functional properties (SWC, WSI, WHC, and OHC) that can be modulated according to temperature. The CPWSP were also noted to display good anti-hypertensive activities. Overall, the results indicate that CPWSP have attractive chemical, biological, and functional properties that make them potential promising candidates for application as alternative additives in various food, cosmetic, and pharmaceutical preparations.
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire Analyses Alimentaires, route de Soukra, 3038 Sfax, Tunisia.
authors: Abir
authors: A
authors: Mokni Ghribi
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Unité Enzymes et Bioconversion, route de Soukra, 3038 Sfax, Tunisia; Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Equipe ProBioGEM, Université Lille 1, France.
authors: Assaâd
authors: A
authors: Sila
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire Analyses Alimentaires, route de Soukra, 3038 Sfax, Tunisia.
authors: Ines
authors: I
authors: Maklouf Gafsi
authors: Université de Liège, Gembloux Agro Bio-Tech, Unité de Technologie des Industries Agro-Alimentaires, passage des Déportés 2, 5030 Gembloux, Belgium.
authors: Christophe
authors: C
authors: Blecker
authors: Université de Liège, Gembloux Agro Bio-Tech, Unité de Technologie des Industries Agro-Alimentaires, passage des Déportés 2, 5030 Gembloux, Belgium.
authors: Sabine
authors: S
authors: Danthine
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire Analyses Alimentaires, route de Soukra, 3038 Sfax, Tunisia.
authors: Hamadi
authors: H
authors: Attia
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Unité Enzymes et Bioconversion, route de Soukra, 3038 Sfax, Tunisia.
authors: Ali
authors: A
authors: Bougatef
authors: Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire Analyses Alimentaires, route de Soukra, 3038 Sfax, Tunisia. Electronic address: besbes.s@voila.fr.
authors: Souhail
authors: S
authors: Besbes
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D029863:Cicer / Q000737:chemistry; D004655:Emulsions; D005433:Flour; D009821:Oils / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004655:Emulsions; D009821:Oils; D011134:Polysaccharides; D014867:Water
keywords: Anti-hypertensive properties; Chickpea water-soluble polysaccharides; Functional properties; Physico-chemical characteristics