title: Comparison of surgical methods of transient middle cerebral artery occlusion between rats and mice.
abstract: Rodent models of focal cerebral ischemia that do not require craniotomy have been developed by intraluminal suture middle cerebral artery occlusion (MCAo). Mouse MCAo models have been widely used and extended to genetic studies of cell death or recovery mechanisms. Therefore, we compared surgery-related parameters and techniques between such rats and mice. In rodent MCAo models, has to be considered body temperature during the operative period, as well as the need for the use of a standardized tip in terms of the outer diameter of probes. Induction of focal cerebral ischemia was measured by neurological dysfunction parameters. Our methods could induce stable moderate-severity ischemic brain injury models and histological alteration at 24 hr after MCAo surgery. Moreover approximately 80% (rats) and 85% (mice) survival ratios were shown indicating with model engineering success. Finally, we described and compared major parameters between rats and mice, including probe size, thread insert length, operation and occlusion periods, and differences in the procedures.
authors: Biohealth Products Research Center (BPRC), Inje University, Gimhae, Korea.
authors: Seunghoon
authors: S
authors: Lee
authors: Yunkyung
authors: Y
authors: Hong
authors: Sookyoung
authors: S
authors: Park
authors: Sang-Rae
authors: SR
authors: Lee
authors: Kyu-Tae
authors: KT
authors: Chang
authors: Yonggeun
authors: Y
authors: Hong
mesh_terms: D000818:Animals; D001831:Body Temperature; D002536:Cerebral Arteries / Q000601:surgery*; D002561:Cerebrovascular Disorders / Q000150:complications / Q000601:surgery*; D004195:Disease Models, Animal; D002546:Ischemic Attack, Transient / Q000209:etiology*; D051379:Mice; D051381:Rats; D014656:Vascular Surgical Procedures / Q000379:methods*
references: Brain Res. 1997 Mar 28;752(1-2):209-18
references: 9106459
references: Neuroreport. 1998 May 11;9(7):1317-9
references: 9631421
references: J Biomed Biotechnol. 2011;2011:464701
references: 21331357
references: Brain Res. 2003 Apr 25;970(1-2):131-9
references: 12706254
references: Neuropathol Appl Neurobiol. 2000 Jun;26(3):232-42
references: 10886681
references: Brain Res. 1999 Mar 20;822(1-2):260-4
references: 10082907
references: Cerebrovasc Dis. 2001;11 Suppl 1:20-30
references: 11244197
references: Stroke. 1989 Jan;20(1):84-91
references: 2643202
references: Nat Cell Biol. 2013 Feb;15(2):126-34
references: 23334500
references: J Vis Exp. 2012;(69). pii: 4038. doi: 10.3791/4038
references: 23168377
references: J Neurosci Methods. 2002 Jan 15;113(1):51-8
references: 11741721
references: ILAR J. 2003;44(2):85-95
references: 12652003
references: Lab Anim Res. 2012 Mar;28(1):47-54
references: 22474474
references: Pharmacol Biochem Behav. 2007 May;87(1):179-97
references: 17521716
references: J Neurochem. 2010 Apr;113(2):303-12
references: 20405577
references: Neural Regen Res. 2012 May 5;7(13):993-9
references: 25722687
references: J Vis Exp. 2011;(48). pii: 2761. doi: 10.3791/2761
references: 21372780
references: Stroke. 1986 May-Jun;17(3):472-6
references: 3715945
references: J Neurosci Methods. 2005 Aug 30;147(1):22-8
references: 16054513
references: Neurobiol Dis. 1999 Apr;6(2):101-8
references: 10343325
references: Mol Neurobiol. 2010 Jun;41(2-3):172-9
references: 20157789
references: Handb Exp Pharmacol. 2009;(190):359-81
references: 19096787
references: Circulation. 2008 Jan 29;117(4):e25-146
references: 18086926
references: Brain Sci. 2012 Oct 16;2(4):483-503
references: 24961257
references: Neurosurgery. 2000 Feb;46(2):448-60; discussion 460-1
references: 10690735
references: Neural Regen Res. 2014 Apr 1;9(7):757-8
references: 25206884
references: J Vis Exp. 2011;(47). pii: 2423. doi: 10.3791/2423
references: 21248698
references: CNS Neurosci Ther. 2011 Dec;17(6):661-9
references: 21129167