title: High-density lipoprotein oxidation in type 2 diabetic patients and young patients with premature myocardial infarction.
abstract: OBJECTIVE
  ApoA-I can undergo oxidative changes that reduce anti-atherogenic role of HDL. The aim of this study was to seek any significant differences in methionine sulfoxide (MetO) content in the ApoA-I of HDL isolated from young patients with coronary heart disease (CHD), type 2 diabetics and healthy subjects.
  
  
  RESULTS
  We evaluated the lipid profile of 21 type 2 diabetic patients, 23 young patients with premature MI and 21 healthy volunteers; we determined in all patients the MetO content of ApoA-I in by MALDI/TOF/TOF technique. The typical MALDI spectra of the tryptic digest obtained from HDL plasma fractions all patients showed a relative abundance of peptides containing Met(112)O in ApoA-I in type 2 diabetic and CHD patients. This relative abundance is given as percentages of oxidized ApoA-I (OxApoA-I). OxApoA-I showed no significant correlations with lipoproteins in all patients studied, while a strong correlation emerged between the duration of diabetic disease and OxApoA-I levels in type 2 diabetic patients.
  
  
  CONCLUSIONS
  The most remarkable finding of our study lies in the evidence it produced of an increased HDL oxidation in patients highly susceptible to CHD. Levels of MetO residues in plasma ApoA-I, measured using an accurate, specific method, should be investigated and considered in prospective future studies to assess their role in CHD.
authors: Department of Medicine - DIMED, University of Padova, Italy.
authors: G
authors: G
authors: Sartore
authors: CNR-IENI, Padova, Italy.
authors: R
authors: R
authors: Seraglia
authors: Department of Medicine - DIMED, University of Padova, Italy. Electronic address: silvia.burlina@studenti.unipd.it.
authors: S
authors: S
authors: Burlina
authors: Department of Medicine - DIMED, University of Padova, Italy.
authors: A
authors: A
authors: Bolis
authors: Department of Medicine - DIMED, University of Padova, Italy.
authors: R
authors: R
authors: Marin
authors: Department of Medicine - DIMED, University of Padova, Italy.
authors: E
authors: E
authors: Manzato
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Italy.
authors: E
authors: E
authors: Ragazzi
authors: CNR-IENI, Padova, Italy.
authors: P
authors: P
authors: Traldi
authors: Department of Medicine - DIMED, University of Padova, Italy.
authors: A
authors: A
authors: Lapolla
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*; D018384:Oxidative Stress; D012307:Risk Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008715:Methionine; C013111:methionine sulfoxide
keywords: Apolipoprotein A-I; HDL oxidation; Type 2 diabetes