title: Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi.
abstract: Genomics has revolutionized the research on fungal secondary metabolite (SM) biosynthesis. To elucidate the molecular and enzymatic mechanisms underlying the biosynthesis of a specific SM compound, the important first step is often to find the genes that responsible for its synthesis. The accessibility to fungal genome sequences allows the bypass of the cumbersome traditional library construction and screening approach. The advance in next-generation sequencing (NGS) technologies have further improved the speed and reduced the cost of microbial genome sequencing in the past few years, which has accelerated the research in this field. Here, we will present an example work flow for identifying the gene cluster encoding the biosynthesis of SMs of interest using an NGS approach. We will also review the different strategies that can be employed to pinpoint the targeted gene clusters rapidly by giving several examples stemming from our work.
authors: Chemical and Biomolecular Engineering Department, University of California Los Angeles, Los Angeles, CA, USA.
authors: Ralph A
authors: RA
authors: Cacho
authors: Chemical and Biomolecular Engineering Department, University of California Los Angeles, Los Angeles, CA, USA ; Chemistry and Biochemistry Department, University of California Los Angeles, Los Angeles, CA, USA.
authors: Yi
authors: Y
authors: Tang
authors: Plant Sciences Division, Research School of Biology, The Australian National University Canberra, ACT, Australia.
authors: Yit-Heng
authors: YH
authors: Chooi
keywords: filamentous fungi; gene clusters; genome mining; next generation sequencing; secondary metabolites
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