title: Presentation of a nano-based tag for immunoassay, based on amine-modified bovine serum albumin nanoparticles.
abstract: The aim of this study was to evaluate four immunoassays, based on amine-modified bovine serum albumin nanoparticles (AMBSANPs). First, the capability of nitrate absorption by AMBSANPs under different conditions was evaluated. Then, serial concentrations of pure βHCG were added to wells coated with βHCG antibody for immunoassays 1 and 2, and wells coated with βHCG aptamer for immunoassays 3 and 4. Next, AMBSANPs conjugated with βHCG antibody was added for immunoassays 1 and 3, and AMBSANPs conjugated with βHCG aptamer were added for immunoassays 2 and 4. Finally, the optical density (OD) of each well was read at 340 nm, and compared with controls. Moreover, the concentration of βHCG in the clinical samples was quantified by immunoassays 1, 2, 3, 4 and ELISA, and then compared. The effect of some serum interferences on these immunoassay methods was evaluated. The authors observed that the amount of nitrate absorption by AMBSANPs increased with an increase in H+ ion concentration and temperature, and decreased with an increase in ion strength. The correlation (R2) between ELISA and immunoassays 1, 2, 3 and 4 were 0.97, 0.97, 0.98, 0.99, respectively. It was found that the increase in the serum interferences led to a decrease in the measured βHCG concentration.
authors: Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran.
authors: Ali
authors: A
authors: Jebali
authors: Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors: Seyedhossein
authors: S
authors: Hekmatimoghaddam
authors: Department of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. bahram_14@yahoo.com.
authors: Bahram
authors: B
authors: Kazemi
authors: Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Translation Medicine, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People's Republic of China.
authors: Jesus Martinez
authors: JM
authors: De La Fuente
mesh_terms: D000328:Adult; D000588:Amines; D055910:Antibodies, Immobilized / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008875:Middle Aged; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000588:Amines; D055910:Antibodies, Immobilized; D006063:Chorionic Gonadotropin; D012710:Serum Albumin, Bovine