title: Influence of L-arginine during bovine in vitro fertilization.
abstract: The objective of this work was to evaluate the effect of using L-arginine during in vitro fertilization (IVF) on in vitro embryonic development using Bos taurus and Bos indicus semen. Effect of different concentrations (0, 1, 10 and 50 mM) of L-arginine, added to the IVF medium, was evaluated on the fertilization rate at 18 h post-fertilization (hpf), NO3(-)/NO2(-) production during IVF by the Griess colorimetric method (30 hpf), cleavage and blastocyst rates (on Day 2 and Day 7 of culture, respectively) and total blastocyst cell number (Day 7 of culture). The results reveal that the addition of 50 mM L-arginine to IVF medium, with either Bos taurus or Bos indicus spermatozoa, decreased the cleavage rate and blastocyst rate compared to the control group. Other concentrations did not affect embryo production. However, 1 mM L-arginine with Bos indicus semen increased the proportion of hatched blastocysts. These results indicate that high L-arginine concentrations may exhibit toxic effects on bovine gametes during in vitro fertilization.
authors: Thiago Velasco Guimarães
authors: TV
authors: Silva
authors: Bruno Baraúna
authors: BB
authors: da Silva
authors: André Luiz Alves
authors: AL
authors: de Sá
authors: Nathalia Nogueira
authors: NN
authors: da Costa
authors: Rafael Vilar
authors: RV
authors: Sampaio
authors: Marcela da Silva
authors: Mda S
authors: Cordeiro
authors: Priscila Di Paula Bessa
authors: PD
authors: Santana
authors: Paulo Roberto
authors: PR
authors: Adona
authors: Simone do Socorro Damasceno
authors: Sdo S
authors: Santos
authors: Moysés dos Santos
authors: Mdos S
authors: Miranda
authors: Otávio Mitio
authors: OM
authors: Ohashi
mesh_terms: D000818:Animals; D001120:Arginine; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro / Q000187:drug effects*; D008297:Male; D008856:Microscopy, Fluorescence; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D009566:Nitrates; D009573:Nitrites; D001120:Arginine