title: Rapid formation of a supramolecular polypeptide-DNA hydrogel for in situ three-dimensional multilayer bioprinting.
abstract: A rapidly formed supramolecular polypeptide-DNA hydrogel was prepared and used for in situ multilayer three-dimensional bioprinting for the first time. By alternative deposition of two complementary bio-inks, designed structures can be printed. Based on their healing properties and high mechanical strengths, the printed structures are geometrically uniform without boundaries and can keep their shapes up to the millimeter scale without collapse. 3D cell printing was demonstrated to fabricate live-cell-containing structures with normal cellular functions. Together with the unique properties of biocompatibility, permeability, and biodegradability, the hydrogel becomes an ideal biomaterial for 3D bioprinting to produce designable 3D constructs for applications in tissue engineering.
authors: Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084 (China).
authors: Chuang
authors: C
authors: Li
authors: Alan
authors: A
authors: Faulkner-Jones
authors: Alison R
authors: AR
authors: Dun
authors: Juan
authors: J
authors: Jin
authors: Ping
authors: P
authors: Chen
authors: Yongzheng
authors: Y
authors: Xing
authors: Zhongqiang
authors: Z
authors: Yang
authors: Zhibo
authors: Z
authors: Li
authors: Wenmiao
authors: W
authors: Shu
authors: Dongsheng
authors: D
authors: Liu
authors: Rory R
authors: RR
authors: Duncan
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D062028:Bioprinting / Q000379:methods*; D002468:Cell Physiological Phenomena; D002477:Cells / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000138:chemical synthesis*; D010539:Permeability; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D004247:DNA; D003851:Deoxyribonucleases
keywords: DNA structures; biodegradability; hydrogels; polypeptides; supramolecular chemistry