title: Dopaminergic modulation of locomotor network activity in the neonatal mouse spinal cord.
abstract: Dopamine is now well established as a modulator of locomotor rhythms in a variety of developing and adult vertebrates. However, in mice, while all five dopamine receptor subtypes are present in the spinal cord, it is unclear which receptor subtypes modulate the rhythm. Dopamine receptors can be grouped into two families-the D1/5 receptor group and the D2/3/4 group, which have excitatory and inhibitory effects, respectively. Our data suggest that dopamine exerts contrasting dose-dependent modulatory effects via the two receptor families. Our data show that administration of dopamine at concentrations >35 μM slowed and increased the regularity of a locomotor rhythm evoked by bath application of 5-hydroxytryptamine (5-HT) and N-methyl-d(l)-aspartic acid (NMA). This effect was independent of the baseline frequency of the rhythm that was manipulated by altering the NMA concentration. We next examined the contribution of the D1- and D2-like receptor families on the rhythm. Our data suggest that the D1-like receptor contributes to enhancement of the stability of the rhythm. Overall, the D2-like family had a pronounced slowing effect on the rhythm; however, quinpirole, the D2-like agonist, also enhanced rhythm stability. These data indicate a receptor-dependent delegation of the modulatory effects of dopamine on the spinal locomotor pattern generator.
authors: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada;
authors: Simon A
authors: SA
authors: Sharples
authors: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada;
authors: Jennifer M
authors: JM
authors: Humphreys
authors: Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina; and.
authors: A Marley
authors: AM
authors: Jensen
authors: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada;
authors: Sunny
authors: S
authors: Dhoopar
authors: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada;
authors: Nicole
authors: N
authors: Delaloye
authors: Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina; and.
authors: Stefan
authors: S
authors: Clemens
authors: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, Alberta, Canada whelan@ucalgary.ca.
authors: Patrick J
authors: http://orcid.org/0000-0002-1234-5415
authors: PJ
authors: Whelan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004298:Dopamine / Q000494:pharmacology / Q000502:physiology*; D008124:Locomotion / Q000187:drug effects; D051379:Mice; D017447:Receptors, Dopamine D1 / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*
chemical_list: D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D004298:Dopamine
keywords: dopamine; locomotion; monoamine; spinal cord
references: Neuroscience. 2007 Nov 23;149(4):885-97
references: 17936519
references: Front Neural Circuits. 2014;8:55
references: 24982614
references: Front Neural Circuits. 2014;8:62
references: 24966815
references: Prog Brain Res. 2002;137:125-39
references: 12440364
references: Physiology (Bethesda). 2011 Dec;26(6):393-411
references: 22170958
references: J Physiol. 2009 Apr 1;587(Pt 7):1499-511
references: 19204052
references: Front Neural Circuits. 2010 Aug 06;4:null
references: 20844601
references: J Neurophysiol. 2006 Dec;96(6):3122-9
references: 16956991
references: Brain Res Bull. 2000 Nov 15;53(5):689-710
references: 11165804
references: Nat Commun. 2011;2:274
references: 21505430
references: Neurosci Lett. 1988 Nov 22;94(1-2):5-9
references: 3071747
references: Acta Physiol Scand. 1967 Jul-Aug;70(3):389-402
references: 4294400
references: Brain Res. 1992 Dec 11;598(1-2):233-41
references: 1486484
references: Brain Res Bull. 1993;30(3-4):319-24
references: 8457880
references: J Comp Neurol. 1996 Dec 23;376(4):631-52
references: 8978475
references: J Neurophysiol. 1997 Jan;77(1):247-59
references: 9120567
references: Neuropharmacology. 2013 Apr;67:370-8
references: 23238327
references: Neurosci Lett. 1993 Aug 20;158(2):217-20
references: 8233099
references: Brain Res. 2003 Apr 25;970(1-2):1-8
references: 12706243
references: J Neurophysiol. 1996 Apr;75(4):1472-82
references: 8727391
references: J Physiol. 1992 Sep;455:187-204
references: 1362441
references: Nature. 1995 Mar 16;374(6519):266-8
references: 7885446
references: Brain Res. 1998 May 25;794(1):169-73
references: 9630613
references: J Neurosci. 1996 Sep 15;16(18):5777-94
references: 8795632
references: J Neurophysiol. 2000 Dec;84(6):2821-33
references: 11110812
references: J Neurophysiol. 2012 Apr;107(8):2250-9
references: 22262823
references: J Neurosci. 2007 Nov 28;27(48):13192-204
references: 18045913
references: J Neurophysiol. 1995 Sep;74(3):1109-17
references: 7500136
references: J Recept Signal Transduct Res. 2004 Aug;24(3):165-205
references: 15521361
references: Brain Res. 1991 Apr 19;546(2):250-60
references: 2070262
references: J Neurophysiol. 2012 Dec;108(12):3370-81
references: 22993259
references: J Neurophysiol. 1999 Aug;82(2):741-6
references: 10444672
references: Eur J Pharmacol. 1986 Nov 19;131(2-3):301-6
references: 3493161
references: J Neurosci. 2000 Sep 1;20(17):6666-71
references: 10964971
references: J Neurophysiol. 1997 Mar;77(3):1155-70
references: 9084588
references: J Neurophysiol. 2004 Sep;92(3):1566-76
references: 15163678
references: J Neurophysiol. 2011 Mar;105(3):1212-24
references: 21228305
references: J Neurosci. 1996 Oct 15;16(20):6579-91
references: 8815934
references: Acta Physiol Scand. 1967 Jul-Aug;70(3):369-88
references: 4293473
references: J Physiol. 2010 Jan 1;588(Pt 1):139-56
references: 19884315
references: FASEB J. 1988 Apr;2(7):2283-8
references: 2450802
references: Neuroscience. 2009 Feb 18;158(4):1699-707
references: 19110039
references: Neurosci Lett. 1994 Jan 17;166(1):23-6
references: 8190353
references: Exp Brain Res. 2006 Jan;168(1-2):152-6
references: 16044299
references: Physiol Rev. 1998 Jan;78(1):189-225
references: 9457173
references: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1945-9
references: 8700864
references: Front Neural Circuits. 2014;8:77
references: 25071458
references: Eur J Neurosci. 2003 Dec;18(11):2919-24
references: 14656287
references: ScientificWorldJournal. 2007;7:58-63
references: 17982577
references: Brain Res. 1999 Jan 23;816(2):493-9
references: 9878874
references: J Neurophysiol. 2003 Aug;90(2):531-8
references: 12904484
references: Brain Res Dev Brain Res. 1997 May 20;100(1):130-2
references: 9174256
references: J Neurophysiol. 1997 Jan;77(1):289-98
references: 9120571
references: Eur J Neurosci. 2004 Mar;19(5):1325-35
references: 15016090
references: Nat Rev Neurosci. 2003 Jul;4(7):573-86
references: 12838332
references: Neurosci Lett. 2000 Feb 25;280(3):187-90
references: 10675792
references: J Physiol. 2008 May 15;586(10):2487-97
references: 18372309
references: J Neurophysiol. 2007 Nov;98(5):2807-17
references: 17715187