title: Sepsis Pathogen Identification.
abstract: Sepsis is a rapidly progressing, severe inflammatory response to infection, causing more than 200,000 deaths per year. Rapid, specific pathogen identification is important to guide sepsis treatment. In this review, we describe and compare currently available commercial products for sepsis diagnosis and pathogen identification, based on microbiological, molecular, and mass spectrometric technologies. Microbiological techniques, the current "gold standard" in sepsis pathogen identification, include blood culture followed by subculturing and pathogen identification via biochemical or microscopic means. These methods have been automated but nevertheless require several days to generate results. Alternative technologies, including highly multiplexed PCR-based methods and mass spectrometric approaches, can decrease the required turnaround time. Matrix-assisted laser-desorption ionization time-of-flight-based systems have recently become an attractive option to rapidly identify a broad spectrum of sepsis pathogens with good sensitivity and specificity. Effectively integrating rapid sepsis pathogen identification into the hospital workflow can improve patient outcomes and can reduce the length of hospitalization and cost per patient.
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Katy
authors: K
authors: Chun
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Chas
authors: C
authors: Syndergaard
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Carlos
authors: C
authors: Damas
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Richard
authors: R
authors: Trubey
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Amruthavani
authors: A
authors: Mukindaraj
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Shenyu
authors: S
authors: Qian
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Xin
authors: X
authors: Jin
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA.
authors: Scott
authors: S
authors: Breslow
authors: Keck Graduate Institute of Applied Life Sciences, Claremont, CA, USA aniemz@kgi.edu.
authors: Angelika
authors: A
authors: Niemz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D057205:Automation, Laboratory; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D003937:Diagnosis, Differential; D005658:Fungi / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing / Q000639:trends; D016533:Mycological Typing Techniques / Q000639:trends; D009181:Mycoses / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D018805:Sepsis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D012772:Shock, Septic / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D014780:Viruses / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents
keywords: mass spectrometry; microbiology; nucleic acid testing; sepsis