title: Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques.
abstract: Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P < 0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species.
authors: Forest Genomics and Informatics Laboratory (fGiL), Department of Molecular Biology, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak Malaysia.
authors: Kit-Siong
authors: KS
authors: Liew
authors: Forest Genomics and Informatics Laboratory (fGiL), Department of Molecular Biology, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak Malaysia.
authors: Wei-Seng
authors: WS
authors: Ho
authors: Applied Forest Science and Industry Development (AFSID), Sarawak Forestry Corporation, 93250 Kuching, Sarawak Malaysia.
authors: Shek-Ling
authors: SL
authors: Pang
authors: Applied Forest Science and Industry Development (AFSID), Sarawak Forestry Corporation, 93250 Kuching, Sarawak Malaysia.
authors: Abdullah
authors: A
authors: Julaihi
keywords: Duabanga moluccana; ISSR-PCR; Microsatellite markers; Planted forest; Population genetics
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