title: Specific enrichment of phosphoproteins using functionalized multivalent nanoparticles.
abstract: Analysis of protein phosphorylation remains a significant challenge due to the low abundance of phosphoproteins and the low stoichiometry of phosphorylation, which requires effective enrichment of phosphoproteins. Here we have developed superparamagnetic nanoparticles (NPs) whose surface is functionalized by multivalent ligand molecules that specifically bind to the phosphate groups on any phosphoproteins. These NPs enrich phosphoproteins from complex cell and tissue lysates with high specificity as confirmed by SDS-PAGE analysis with a phosphoprotein-specific stain and mass spectrometry analysis of the enriched phosphoproteins. This method enables universal and effective capture, enrichment, and detection of intact phosphoproteins toward a comprehensive analysis of the phosphoproteome.
authors: Department of Chemistry, ‡Department of Cell and Regenerative Biology, §Molecular and Cellular Pharmacology Program, and ∥Human Proteomics Program, University of Wisconsin-Madison , Madison, Wisconsin 53719, United States.
authors: Leekyoung
authors: L
authors: Hwang
authors: Serife
authors: S
authors: Ayaz-Guner
authors: Zachery R
authors: ZR
authors: Gregorich
authors: Wenxuan
authors: W
authors: Cai
authors: Santosh G
authors: SG
authors: Valeja
authors: Song
authors: S
authors: Jin
authors: Ying
authors: Y
authors: Ge
mesh_terms: D008024:Ligands; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D058185:Magnetite Nanoparticles; D010750:Phosphoproteins
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