title: miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells.
abstract: Human telomerase reverse transcriptase (hTERT) plays a crucial role in cancer development. We previously identified paired-like homeodomain1 (PITX1) as an hTERT suppressor gene. However, the underlying mechanisms that are involved in the regulation of PITX1 remain unknown. Here, we report that the microRNA-19b (miR-19b) regulates hTERT expression and cell proliferation through inhibition of PITX1. Compared with normal melanocyte cells, miR-19b expression was higher in most melanoma cells and was accompanied by downregulation of PITX1. Moreover, overexpression of miR-19b inhibited PITX1 mRNA translation through a miR-19b binding site within the 3'UTR of the PITX1 mRNA. Our combined findings indicate the participation of miR-19b as a novel upstream effector of hTERT transcription via direct targeting of PITX1.
authors: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Takahito
authors: T
authors: Ohira
authors: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Sunamura
authors: S
authors: Naohiro
authors: Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Yuji
authors: Y
authors: Nakayama
authors: 1] Division of Pathological Biochemistry, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan [2] Chromosome Engineering Research Center, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Mitsuhiko
authors: M
authors: Osaki
authors: 1] Division of Pathological Biochemistry, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan [2] Chromosome Engineering Research Center, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Futoshi
authors: F
authors: Okada
authors: Chromosome Engineering Research Center, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Mitsuo
authors: M
authors: Oshimura
authors: 1] Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan [2] Chromosome Engineering Research Center, Tottori University, Yonago, Tottori 683-8503, Japan.
authors: Hiroyuki
authors: H
authors: Kugoh
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D051761:Paired Box Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016415:Sequence Alignment; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; C101485:homeobox protein PITX1; C509186:TERT protein, human; D019098:Telomerase
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