title: Synthesis and characterization of Bombesin-superparamagnetic iron oxide nanoparticles as a targeted contrast agent for imaging of breast cancer using MRI.
abstract: The targeted delivery of superparamagnetic iron oxide nanoparticles (SPIONs) as a contrast agent may facilitate their accumulation in cancer cells and enhance the sensitivity of MR imaging. In this study, SPIONs coated with dextran (DSPIONs) were conjugated with bombesin (BBN) to produce a targeting contrast agent for detection of breast cancer using MRI. X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer analyses indicated the formation of dextran-coated superparamagnetic iron oxide nanoparticles with an average size of 6.0 ± 0.5 nm. Fourier transform infrared spectroscopy confirmed the conjugation of the BBN with the DSPIONs. A stability study proved the high optical stability of DSPION-BBN in human blood serum. DSPION-BBN biocompatibility was confirmed by cytotoxicity evaluation. A binding study showed the targeting ability of DSPION-BBN to bind to T47D breast cancer cells overexpressing gastrin-releasing peptide (GRP) receptors. T2-weighted and T2*-weighted color map MR images were acquired. The MRI study indicated that the DSPION-BBN possessed good diagnostic ability as a GRP-specific contrast agent, with appropriate signal reduction in T2*-weighted color map MR images in mice with breast tumors.
authors: Nanostructure Lab, Physics Department, University of Guilan, Rasht, Iran.
authors: Atefeh
authors: A
authors: Jafari
authors: Mojtaba
authors: M
authors: Salouti
authors: Saber Farjami
authors: SF
authors: Shayesteh
authors: Zahra
authors: Z
authors: Heidari
authors: Ahmad Bitarafan
authors: AB
authors: Rajabi
authors: Komail
authors: K
authors: Boustani
authors: Ali
authors: A
authors: Nahardani
mesh_terms: D000818:Animals; D001839:Bombesin / Q000737:chemistry*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D019886:Gastrin-Releasing Peptide / Q000097:blood; D006801:Humans; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D018377:Neurotransmitter Agents / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D018377:Neurotransmitter Agents; D010455:Peptides; C000499:ferric oxide; D019886:Gastrin-Releasing Peptide; D001839:Bombesin