title: Construction and analysis of antennal cDNA library from rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), and expression profiles of putative odorant-binding protein and chemosensory protein genes.
abstract: In this study, we constructed a high-quality cDNA library from the antennae of the Chilo suppressalis (Walker) (Lepidoptera: Pyralidae). A total of 1,235 colonies with inserts greater than 0.7 kb were sequenced and analyzed. Homology searching coupled with bioinformatics analysis identified 15 and 7 cDNA sequences, respectively, encoding putative odorant-binding proteins (OBPs) and chemosensory proteins (CSPs). A phylogenetic tree of CsupCSPs showed that each CsupCSP has orthologs in Manduca sexta and Bombyx mori with strong bootstrapping support. One CSP was either very specific or more related to the CSPs of another species than to conspecific CSP. The expression profiles of the OBPs and CSPs in different tissues were measured by real-time quantitative PCR. The results revealed that of the 11 OBP genes, the transcript levels of CsupOBP1, CsupOBP5, and CsupOBP7 were higher in both male and female antennae than those in other tissues. And CsupCSP7 was highly expressed in both male and female antennae. Based on these results, the possible physiological functions of CsupOBPs and CsupCSPs were discussed.
authors: State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China; Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Hangzhou, China; Institute of Insect Sciences, Zhejiang University, Hangzhou, China; Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China.
authors: Zhong-Jun
authors: ZJ
authors: Gong
authors: Su
authors: S
authors: Liu
authors: Yan-Dong
authors: YD
authors: Jiang
authors: Wen-Wu
authors: WW
authors: Zhou
authors: Qing-Mei
authors: QM
authors: Liang
authors: Jiaan
authors: J
authors: Cheng
authors: Chuan-Xi
authors: CX
authors: Zhang
authors: Zeng-Rong
authors: ZR
authors: Zhu
authors: Geoff M
authors: GM
authors: Gurr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Chilo suppressalis; cDNA library; chemosensory protein; odorant-binding protein; real-time PCR