title: Preparation of molecularly imprinted polymers for the recognition of proteins via the generation of peptide-fragment binding sites by semi-covalent imprinting and enzymatic digestion.
abstract: Molecularly imprinted polymers bearing peptide fragment-based binding sites within the protein-imprinted cavities were prepared by copolymerization of the acrylated protein with 6-monoacryloyl-trehalose and 6,6'-diacryloyl-trehalose as a hydrophilic comonomer and a crosslinker respectively, followed by enzymatic decomposition of the grafted protein into the polymer matrix with pepsin, resulting in the creation of peptide fragment-based protein-binding sites.
authors: Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan. takeuchi@gold.kobe-u.ac.jp.
authors: Hironori
authors: H
authors: Taguchi
authors: Hirobumi
authors: H
authors: Sunayama
authors: Eri
authors: E
authors: Takano
authors: Yukiya
authors: Y
authors: Kitayama
authors: Toshifumi
authors: T
authors: Takeuchi
mesh_terms: D001665:Binding Sites; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D010434:Pepsin A / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014199:Trehalose / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D011108:Polymers; D045304:Cytochromes c; D014199:Trehalose; D010434:Pepsin A