title: Graphene oxide-based optical biosensor functionalized with peptides for explosive detection.
abstract: A label-free optical biosensor was constructed with biofunctionalized graphene oxide (GO) for specific detection of 2,4,6-trinitrotoluene (TNT). By chemically binding TNT-specific peptides with GO, the biosensor gained unique optoelectronic properties and high biological sensitivity, with transducing bimolecular bonding into optical signals. Through UV absorption detection, increasing absorbance responses could be observed in presence of TNT at different concentrations, as low as 4.40×10(-9) mM, and showed dose-dependence and stable behavior. Specific responses of the biosensor were verified with the corporation of 2,6-dinitrotoluene (DNT), which had similar molecular structure to TNT. Thus, with high sensitivity and selectivity, the biosensor provided a convenient approach for detection of explosives as miniaturizing and integrating devices.
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors: Qian
authors: Q
authors: Zhang
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors: Diming
authors: D
authors: Zhang
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors: Yanli
authors: Y
authors: Lu
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors: Yao
authors: Y
authors: Yao
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors: Shuang
authors: S
authors: Li
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China; Cyber Innovation Joint Research Center, Zhejiang University, Hangzhou 310027, PR China. Electronic address: qjliu@zju.edu.cn.
authors: Qingjun
authors: Q
authors: Liu
mesh_terms: D015374:Biosensing Techniques; D053834:Explosive Agents / Q000737:chemistry / Q000302:isolation & purification*; D006108:Graphite / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D014303:Trinitrotoluene / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D053834:Explosive Agents; D010087:Oxides; D010455:Peptides; D014303:Trinitrotoluene; D006108:Graphite
keywords: Biosensor; Explosive; Graphene oxide; Optical detection; Peptide