title: Ozone oxidative preconditioning protects the rat kidney from reperfusion injury via modulation of the TLR4-NF-κB pathway.
abstract: OBJECTIVE
  To investigate the protective effects of ozone oxidative preconditioning (OzoneOP) were associated with the modulation of TLR4-NF-κB pathway.
  
  
  METHODS
  Thirty six rats were subjected to 45 min of renal ischemia, with or without treatment with OzoneOP (1 mg/kg). Blood samples were collected for the detection of blood urea nitrogen and creatinine levels. Histologic examinations were evaluated and immunohistochemistry was also performed for localization of TLR4 and NF-κB. The expression of TNF-α, IL-1β, IL-6, ICAM-1 and MCP-1 were studied by Real-time PCR. Western blot was performed to detect the expression of TLR4 and NF-κB.
  
  
  RESULTS
  The results indicated that blood urea nitrogen and creatinine levels increased significantly in I/R group. Rats treated with OzoneOP showed obviously less renal damage. Immunohistochemistry showed that TLR4 were ameliorated by OzoneOP. Realtime PCR showed that OzoneOP could significantly inhibit the increased mRNA levels of TNF-α, IL-1β, IL-6, ICAM-1 and MCP-1 induced by I/R. Western blot indicated that the expression of TLR4 and NF-κB were upregulated in I/R group, but OzoneOP could inhibit this increase.
  
  
  CONCLUSIONS
  These findings indicated that OzoneOP had potent anti-inflammatory properties by the modulation of the TLR4-NF-κB pathway in renal ischemia/reperfusion injury.
authors: Department of Radiology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Bianzhi
authors: B
authors: Xing
authors: Department of Urology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Hui
authors: H
authors: Chen
authors: Department of Urology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Lei
authors: L
authors: Wang
authors: Department of Urology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Xiaodong
authors: X
authors: Weng
authors: Department of Urology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Zhiyuan
authors: Z
authors: Chen
authors: Department of Urology, Renmin Hospital, Wuhan University, Hubei, China.
authors: Xiuheng
authors: X
authors: Li
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D015153:Blotting, Western; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000032:analysis; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000032:analysis* / Q000378:metabolism; D010126:Ozone / Q000494:pharmacology*; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000517:prevention & control*; D015203:Reproducibility of Results; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000032:analysis* / Q000378:metabolism
chemical_list: D016207:Cytokines; D016328:NF-kappa B; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D018799:Intercellular Adhesion Molecule-1; D010126:Ozone; D003404:Creatinine