title: Isolation, characterization, and optimization of an aerobic butanol-producing bacterium from Singapore.
abstract: We aimed to isolate aerobic butanol-producing microorganisms from environmental samples as potential platform strains for butanol production. Soil samples collected were subjected to a semi-high-throughput screening strategy. A microorganism capable of producing butanol in high concentrations under aerobic conditions was isolated and identified as Bacillus species by 16S rDNA analysis. The growth and butanol production under both aerobic and anaerobic conditions of the isolated Bacillus sp. 15, together with a different composition of by-products, suggest different metabolic networks from the obligate anaerobes Clostridia. At 1 L scale fermentation with 0.2 L/Min of ariflow, butanol titer reached up to 10.38 g/L in a batch culture. The fermentation process of the isolate also occurred in two phases and the acidic condition is critical for butanol production. The butanol concentration was further improved to 12.3 g/L with minimized by-products using a microaerobic condition. With the above-mentioned distinct features, the isolated Bacillus sp. 15 is a suitable platform strain for further process development and metabolic engineering for butanol production.
authors: Institute of Chemical and Engineering Sciences, Jurong Island, Singapore.
authors: Cheng Ying Chloe
authors: CY
authors: Ng
authors: Mitsui Chemical Singapore R&D Centre, Singapore.
authors: Katsuyuki
authors: K
authors: Takahashi
authors: Institute of Chemical and Engineering Sciences, Jurong Island, Singapore.
authors: Zhibin
authors: Z
authors: Liu
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000332:Aerobiosis; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D012846:Singapore; D012988:Soil Microbiology
chemical_list: D020001:1-Butanol
keywords: Bacillus; fermentation; microaerobic; n-butanol