title: Phosphorylcholine-modified chitosan films as effective promoters of cell aggregation: correlation between the films properties and cellular response.
abstract: This study describes chitosan-phosphorylcholine (CH-PC) films able to support the formation of cell aggregates (spheroids), which are important for tissue engineering and pharmacological studies. The surface topography, charge, thickness, and rheology of CH-PC thin films were characterized by AFM, zeta-potential measurements, SPR spectroscopy, and QCM-D measurements. The CH-PC films are highly hydrated gels, independently of the level of PC incorporation (15-40 mol-% PC/glucosamine units). QCM-D studies established that the amount of fibrinogen adsorbed on CH-PC films decreased with increasing PC content. CH-PC surfaces underwent a transition from moderately cell-adhesive (CH-PC15) to non-adhesive (CH-PC40). Optical micrographs of HUVEC and MCF-7 cell lines cultured on CH-PC surfaces showed that they form spheroids on CH-PC25 and CH-PC40 films.
authors: Faculté de Pharmacie and Département de Chimie, Université de Montreal, CP 6128 Succursale Centre Ville, Montreal, QC H3C 3J7, Canada.
authors: Baowen
authors: B
authors: Qi
authors: Piotr
authors: P
authors: Kujawa
authors: Sayaka
authors: S
authors: Toita
authors: Grégory
authors: G
authors: Beaune
authors: Françoise M
authors: FM
authors: Winnik
mesh_terms: D002449:Cell Aggregation / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D061986:MCF-7 Cells; D010767:Phosphorylcholine / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering
chemical_list: D010767:Phosphorylcholine; D048271:Chitosan
keywords: adsorption; biopolymers; chitosan; polyelectroylte; thin films