title: Osteogenesis and mineralization in a rabbit mandibular distraction osteogenesis model is promoted by the human LMP-1 gene.
abstract: To observe the effects of LIM mineralization protein-1 (LMP-1) on bone regeneration in the distraction zone based on gene transduction, 36 New Zealand white rabbits underwent mandibular lengthening with a distraction rate of 2 mm/day. The animals were then randomly divided into group A and group B (n = 18, each). At the end of the distraction, Ad5-EGFP viruses and Ad5-LMP-1/EGFP viruses were injected into the distraction gaps in groups A and B, respectively. Seven days later, five randomly selected animals from each group were sacrificed to evaluate the survival of the virus. Four and 8 weeks after distraction osteogenesis (DO), six samples randomly selected from each group underwent CT scanning and dual energy X-ray absorptiometry detection. Eight weeks after DO, the rabbits were sacrificed, and the distracted mandibles were harvested. Six animals from each group processed for radiography, micro-CT, histology, and the rest samples were taken three-point bend testing. Using this model, better bone formation and mineralization in the distracted callus were observed in group B when compared with those in group A. The results suggest local transduction with LMP-1 gene promotes osteogenesis and mineralization in DO.
authors: Department of Stomatology, The First People's Hospital of Chenzhou, University of China South, No. 102 Luojiajing Road, Chenzhou, Hunan Province, 423000, China.
authors: Xiaowen
authors: X
authors: Jiang
authors: Yanzhe
authors: Y
authors: Chen
authors: Xiaosheng
authors: X
authors: Fan
authors: Hao
authors: H
authors: Zhang
authors: Lu
authors: L
authors: Kun
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001696:Biomechanical Phenomena; D002113:Calcification, Physiologic / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D060588:LIM Domain Proteins / Q000378:metabolism*; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology / Q000502:physiology* / Q000601:surgery*; D023421:Models, Animal; D019857:Osteogenesis, Distraction; D011817:Rabbits; D011859:Radiography; D011897:Random Allocation; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; C484380:PDLIM7 protein, human
keywords: LIM mineralization protein; adenovirus; distraction osteogenesis; mineralization; osteogenesis