title: Ecdysone receptor (EcR) is involved in the transcription of cell cycle genes in the silkworm.
abstract: EcR (ecdysone receptor)-mediated ecdysone signaling pathway contributes to regulate the transcription of genes involved in various processes during insect development. In this work, we detected the expression of EcR gene in silkworm ovary-derived BmN4 cells and found that EcR RNAi result in an alteration of cell shape, indicating that EcR may orchestrate cell cycle progression. EcR RNAi and EcR overexpression analysis revealed that in the cultured BmN4 cells, EcR respectively promoted and suppressed the transcription of E2F-1 and CycE, two genes controlling cell cycle progression. Further examination demonstrated that ecdysone application in BmN4 cells not only changed the transcription of these two cell cycle genes like that under EcR overexpression, but also induced cell cycle arrest at G2/M phase. In vivo analysis confirmed that E2F-1 expression was elevated in silk gland of silkworm larvae after ecdysone application, which is same as its response to ecdysone in BmN4 cells. However, ecdysone also promotes CycE transcription in silk gland, and this is converse with the observation in BmN4 cells. These results provide new insights into understanding the roles of EcR-mediated ecdysone signaling in the regulation of cell cycle.
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. wenliang20081103@126.com.
authors: Wenliang
authors: W
authors: Qian
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. hnklx1986@163.com.
authors: Lixia
authors: L
authors: Kang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. zhangtianlei2011@sina.com.
authors: Tianlei
authors: T
authors: Zhang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. keven190@sina.com.
authors: Meng
authors: M
authors: Meng
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. wangyonghu2006@163.com.
authors: Yonghu
authors: Y
authors: Wang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. lizhiqing@swu.edu.cn.
authors: Zhiqing
authors: Z
authors: Li
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. xiaqy@swu.edu.cn.
authors: Qingyou
authors: Q
authors: Xia
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. chengdj@swu.edu.cn.
authors: Daojun
authors: D
authors: Cheng
mesh_terms: D000818:Animals; D012831:Bombyx / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004440:Ecdysone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D034622:RNA Interference; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D011987:Receptors, Steroid; C055469:ecdysone receptor; D004440:Ecdysone
references: Arch Insect Biochem Physiol. 2000 Feb;43(2):87-96
references: 10644973
references: Genome Res. 2009 Jun;19(6):1006-13
references: 19237466
references: J Insect Physiol. 2010 Oct;56(10):1396-401
references: 20303973
references: BMC Dev Biol. 2013;13:28
references: 23848468
references: PLoS One. 2011;6(10):e25708
references: 21991338
references: Mech Dev. 2008 Jan-Feb;125(1-2):91-105
references: 18036785
references: Annu Rev Entomol. 2013;58:497-516
references: 23072462
references: Semin Cell Dev Biol. 2005 Jun;16(3):311-21
references: 15840440
references: Curr Opin Cell Biol. 2001 Dec;13(6):731-7
references: 11698190
references: Curr Opin Cell Biol. 1998 Dec;10(6):784-90
references: 9914170
references: Development. 1994 Nov;120(11):3131-43
references: 7720557
references: Trends Genet. 1996 Aug;12(8):306-10
references: 8783940
references: Arch Insect Biochem Physiol. 1996;33(3-4):231-44
references: 8913033
references: Bioessays. 1995 Jun;17(6):471-80
references: 7575488
references: PLoS Biol. 2009 Apr 7;7(4):e1000079
references: 19355788
references: Development. 2008 Aug;135(16):2707-16
references: 18614577
references: Cell. 1991 Oct 4;67(1):59-77
references: 1913820
references: Cell Cycle. 2012 Jan 1;11(1):57-64
references: 22186781
references: Nat Rev Genet. 2005 Apr;6(4):311-23
references: 15803199
references: Insect Mol Biol. 2005 Apr;14(2):151-61
references: 15796748
references: Genes Dev. 1995 Jun 15;9(12):1456-68
references: 7601350
references: Genes Dev. 1995 Jun 15;9(12):1445-55
references: 7601349
references: Cell. 1994 Apr 8;77(1):107-20
references: 8156587
references: Development. 2013 Aug;140(15):3079-93
references: 23861057
references: J Insect Physiol. 2004 Sep;50(9):831-8
references: 15350503
references: J Insect Physiol. 2012 Mar;58(3):293-302
references: 22310011
references: Trends Genet. 1992 Apr;8(4):132-8
references: 1631956
references: Trends Endocrinol Metab. 2005 Jan-Feb;16(1):6-11
references: 15620543
references: Mol Cell Biol. 2000 May;20(10):3633-9
references: 10779353
references: Annu Rev Entomol. 2002;47:467-500
references: 11729082
references: Insect Biochem Mol Biol. 2001 Mar 15;31(4-5):349-58
references: 11222944
references: Cancer Res. 1994 Mar 15;54(6):1402-6
references: 8137237
references: Development. 2000 Jul;127(13):2897-905
references: 10851134
references: Methods Mol Biol. 2005;296:69-94
references: 15576927
references: Mol Biol Rep. 2012 May;39(5):5575-88
references: 22187347
references: Cell. 1980 Dec;22(3):675-82
references: 6780197
references: Prog Cell Cycle Res. 1997;3:171-91
references: 9552414
references: Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):12024-9
references: 9342356
references: Development. 1997 Jul;124(13):2499-506
references: 9216992
references: Cell. 1998 Jun 26;93(7):1183-93
references: 9657151
references: Nat Cell Biol. 2003 Sep;5(9):827-33
references: 12942089
references: RNA Biol. 2012 Jan;9(1):40-6
references: 22293577
references: Nature. 1998 Jul 2;394(6688):82-4
references: 9665132
references: Cell. 2004 Apr 16;117(2):253-64
references: 15084262
references: Genes Dev. 1994 Jul 1;8(13):1514-25
references: 7958836
references: Development. 2004 May;131(10):2241-6
references: 15128665
references: PLoS One. 2012;7(4):e34330
references: 22485166
references: Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11729-34
references: 22753472
references: Science. 1996 Dec 6;274(5293):1646-52
references: 8939845
references: Insect Biochem Mol Biol. 2008 May;38(5):529-39
references: 18405831
references: Cell Div. 2009 Jan 20;4:3
references: 19154610
references: Annu Rev Entomol. 2012;57:83-106
references: 22017307