title: Molecular characterization of three gonadotropin subunits and their expression patterns during ovarian maturation in Cynoglossus semilaevis.
abstract: The endocrine regulation of reproduction in a multiple spawning flatfish with an ovary of asynchronous development remains largely unknown. The objectives of this study were to monitor changes in mRNA expression patterns of three gonadotropin hormone (GTH) subunits (FSHβ, LHβ and CGα) and plasma GTH levels during ovarian maturation of half-smooth tongue sole Cynoglossus semilaevis. Cloning and sequence analysis revealed that the cDNAs of FSHβ, LHβ and CGα were 541, 670 and 685 bp in length, and encode for peptides of 130, 158 and 127 amino acids, respectively. The number of cysteine residues and potential N-linked glycosylation sites of the flatfish GTHs were conserved among teleosts. However, the primary structure of GTHs in Pleuronectiformes appeared to be highly divergent. The FSHβ transcriptional level in the pituitary remained high during the vitellogenic stage while plasma levels of FSH peaked and oocyte development was stimulated. The LHβ expression in the pituitary and ovary reached the maximum level during oocyte maturation stages when the plasma levels of LH peaked. The brain GTHs were expressed at the different ovarian stages. These results suggested that FSH and LH may simultaneously regulate ovarian development and maturation through the brain-pituitary-ovary axis endocrine system in tongue sole.
authors: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China. shibao@ysfri.ac.cn.
authors: Bao
authors: B
authors: Shi
authors: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China. liuxz@ysfri.ac.cn.
authors: Xuezhou
authors: X
authors: Liu
authors: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China. xuyj@ysfri.ac.cn.
authors: Yongjiang
authors: Y
authors: Xu
authors: Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China. 0610wangshan@163.com.
authors: Shanshan
authors: S
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D005412:Flatfishes / Q000254:growth & development*; D037201:Follicle Stimulating Hormone, beta Subunit / Q000097:blood / Q000145:classification / Q000378:metabolism*; D015292:Glycoprotein Hormones, alpha Subunit / Q000097:blood / Q000145:classification / Q000378:metabolism*; D037101:Luteinizing Hormone, beta Subunit / Q000097:blood / Q000145:classification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010053:Ovary / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D010802:Phylogeny; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D015292:Glycoprotein Hormones, alpha Subunit; D037101:Luteinizing Hormone, beta Subunit; D012333:RNA, Messenger
references: Genome Res. 2014 Apr;24(4):604-15
references: 24487721
references: Gen Comp Endocrinol. 2010 Feb 1;165(3):367-89
references: 19505465
references: Gen Comp Endocrinol. 2003 Jul;132(3):369-78
references: 12849959
references: Gen Comp Endocrinol. 2003 Sep;133(2):216-32
references: 12928011
references: Endocrine. 1999 Dec;11(3):205-15
references: 10786817
references: Comp Biochem Physiol A Mol Integr Physiol. 2007 Mar;146(3):342-54
references: 17208478
references: Comp Biochem Physiol B Biochem Mol Biol. 2012 Sep;163(1):59-64
references: 22580269
references: Comp Biochem Physiol B Biochem Mol Biol. 2009 Sep;154(1):62-7
references: 19427917
references: Gen Comp Endocrinol. 2001 May;122(2):117-29
references: 11316417
references: Neuroendocrinology. 2009;89(1):27-37
references: 18758154
references: Gen Comp Endocrinol. 1988 Sep;71(3):459-67
references: 3192069
references: Mar Biotechnol (NY). 2010 Oct;12(5):558-68
references: 19957095
references: Gen Comp Endocrinol. 2012 Mar 1;176(1):70-8
references: 22227219
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: J Mol Endocrinol. 1996 Apr;16(2):171-81
references: 9156520
references: Biol Reprod. 1997 Nov;57(5):1238-44
references: 9369193
references: Gen Comp Endocrinol. 1999 Mar;113(3):413-28
references: 10068502
references: J Biol Chem. 2004 Jan 16;279(3):1899-906
references: 14581462
references: J Mol Recognit. 2000 Sep-Oct;13(5):223-78
references: 10992290
references: Biol Reprod. 2004 Feb;70(2):356-63
references: 14561644
references: Fish Physiol Biochem. 2013 Jun;39(3):671-82
references: 23053612
references: Endocrinology. 2004 Nov;145(11):5210-20
references: 15284199
references: Comp Biochem Physiol B Biochem Mol Biol. 2009 Jul;153(3):288-95
references: 19344778
references: Gen Comp Endocrinol. 2008 May 1;156(3):470-81
references: 18353326
references: Gen Comp Endocrinol. 1997 Nov;108(2):223-36
references: 9356218
references: Gen Comp Endocrinol. 2007 Aug-Sep;153(1-3):323-32
references: 17507016
references: Gen Comp Endocrinol. 2011 Jan 1;170(1):99-109
references: 20858497
references: Fish Physiol Biochem. 2010 Dec;36(4):1001-12
references: 20069358
references: Nat Genet. 2014 Mar;46(3):253-60
references: 24487278
references: Gen Comp Endocrinol. 2012 Sep 15;178(3):463-9
references: 22819935
references: Biol Reprod. 2005 Jun;72(6):1382-96
references: 15728794
references: J Genet Genomics. 2010 Feb;37(2):137-45
references: 20227047
references: Gen Comp Endocrinol. 2008 Aug;158(1):68-76
references: 18558403
references: Gen Comp Endocrinol. 2010 Feb 1;165(3):438-55
references: 19393655
references: Biol Reprod. 1996 Jun;54(6):1375-82
references: 8724367
references: Gen Comp Endocrinol. 1998 Jul;111(1):38-50
references: 9653020
references: Biol Reprod. 1991 Jan;44(1):29-38
references: 2015351
references: Nature. 1994 Jun 9;369(6480):455-61
references: 8202136
references: Gen Comp Endocrinol. 2006 Feb;145(3):263-9
references: 16289183
references: Comp Biochem Physiol A Mol Integr Physiol. 2014 Sep;175:102-9
references: 24892730
references: Mar Biotechnol (NY). 2001 Nov;3(6):590-9
references: 14961331
references: Acta Biochim Pol. 2000;47(3):815-9
references: 11310980
references: Gen Comp Endocrinol. 2009 Jun;162(2):192-202
references: 19327361
references: Gen Comp Endocrinol. 1997 Mar;105(3):379-89
references: 9073500
references: Int Rev Cytol. 2003;225:131-85
references: 12696592
references: Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jun;152(1):107-13
references: 20298809
references: Gen Comp Endocrinol. 2004 Jul;137(3):288-99
references: 15201067
references: Biol Reprod. 2003 Apr;68(4):1324-32
references: 12606486
references: Biol Reprod. 2000 Jul;63(1):308-19
references: 10859273
references: Biol Reprod. 2000 Jul;63(1):222-8
references: 10859263
references: Gen Comp Endocrinol. 2011 Jun 1;172(2):268-76
references: 21420971
references: J Mol Endocrinol. 2007 Feb;38(1-2):99-111
references: 17242173
references: Gen Comp Endocrinol. 2003 Apr;131(2):87-96
references: 12679085
references: Gen Comp Endocrinol. 2012 Jan 15;175(2):329-36
references: 22154644
references: Int J Mol Sci. 2014;15(7):12952-8
references: 25054319
references: J Mol Endocrinol. 1999 Oct;23(2):177-87
references: 10514555
references: Comp Biochem Physiol A Mol Integr Physiol. 2004 Mar;137(3):447-77
references: 15123185
references: Gen Comp Endocrinol. 2010 Feb 1;165(3):412-37
references: 19686749
references: J Endocrinol. 1998 Mar;156(3):529-42
references: 9582510
references: Gen Comp Endocrinol. 1998 Jul;111(1):28-37
references: 9653019
references: Mol Cell Endocrinol. 2007 Apr 15;269(1-2):9-16
references: 17350753
references: Int J Biol Sci. 2011;7(4):452-9
references: 21547062
references: Fish Physiol Biochem. 2012 Jun;38(3):883-97
references: 22109677
references: Gen Comp Endocrinol. 1999 Mar;113(3):436-44
references: 10068504
references: Fish Physiol Biochem. 1989 Jun;7(1-6):169-76
references: 24221769
references: Gen Comp Endocrinol. 1998 Oct;112(1):80-8
references: 9748406
references: Gene. 2012 Feb 1;493(1):92-104
references: 22120536