title: Endochondral ossification model system: designed cell fate of human epiphyseal chondrocytes during long-term implantation.
abstract: The aim of this study is to establish a recapitulation system of human endochondral ossification as a paradigm of developmental engineering. Chondrocytes were isolated from the epiphyseal cartilage of the supernumerary digits of infants with polydactyly. In vivo studies showed that implanted chondrocytes exhibited cartilaginous regeneration over a short period of time and subsequent endochondral ossification with a marrow cavity. Tracing studies revealed that cells of donor origin at the periphery of the cartilage migrated into the center of the cartilage and transformed into osteoblasts, adipocytes, and endothelial cells. Bone marrow was formed through anastomosis with the recipient endothelial system at 13 weeks, and from the migration of recipient hematopoietic cells at 50 weeks. This study provides a human endochondral ossification model system with transdifferentiation of the donor cells at the periphery of the cartilage. J. Cell. Physiol. 230: 1376-1388, 2015. © 2015 Wiley Periodicals, Inc., A Wiley Company.
authors: Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
authors: Michiyo
authors: M
authors: Nasu
authors: Shinichiro
authors: S
authors: Takayama
authors: Akihiro
authors: A
authors: Umezawa
mesh_terms: D001853:Bone Marrow / Q000378:metabolism; D002356:Cartilage / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D054338:Cell Transdifferentiation / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D008954:Models, Biological; D010012:Osteogenesis / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D013997:Time Factors