title: Acute Effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) or Paraquat on Core Temperature in C57BL/6J Mice.
abstract: BACKGROUND
  MPTP and paraquat are two compounds that have been used to model Parkinson's disease in mice. Previous studies in two non-traditional strains of mice have shown that a single dose of MPTP can induce changes in body temperature, while the effects of paraquat have not been examined. Examination of body temperature is important since small fluctuations in an animal's core temperature can significantly affect drug metabolism, and if significant enough can even culminate in an animal's death.
  
  
  OBJECTIVE
  To determine how external heating can alter the survival of C57BL/6J mice following MPTP administration.
  
  
  METHODS
  In this study, we examine the effects of MPTP (4×20 mg/kg, 2 hours apart) and paraquat (2×10 mg/kg/week for 3 weeks) on core temperature of C57BL/6J mice. Correlations of purine and catecholamine levels were also done in mice treated with MPTP.
  
  
  RESULTS
  We find that MPTP induces a significant hypothermia in C57BL/6J mice that reduces their core temperature below the limit of fatal hypothermia. Unlike MPTP, paraquat did not induce a significant hypothermia. Placement of animals on heating pads significantly abrogates the loss of core temperature. In both heated and non-heated conditions, mice treated with MPTP showed a significant depletion of ATP within 2 hours of administration in both striatum and SN that started to recover 2 hours after MPTP administration was complete. Striatal DA and DOPAC are significantly reduced starting 4-6 hours after MPTP.
  
  
  CONCLUSIONS
  The fatal hypothermic effects of MPTP can be abrogated through use of external heating.
authors: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors: Yun
authors: Y
authors: Jiao
authors: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors: Yuchen
authors: Y
authors: Dou
authors: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.|Department of Biochemistry, University of Bath, Bath, UK.
authors: Georgina
authors: G
authors: Lockwood
authors: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors: Amar
authors: A
authors: Pani
authors: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors: Richard
authors: R
authors: Jay Smeyne
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D003342:Corpus Striatum / Q000187:drug effects; D004195:Disease Models, Animal; D020267:MPTP Poisoning / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010269:Paraquat / Q000378:metabolism / Q000633:toxicity*; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D011687:Purines / Q000378:metabolism; D013378:Substantia Nigra / Q000187:drug effects
chemical_list: D011687:Purines; D010269:Paraquat; C030985:purine
keywords: MPTP; Mouse strain; fatal hypothermic temperature; paraquat; temperature
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