title: [Leptin inhibits the differentiation of RAW264.7 macrophages into osteoclasts via depressing the expression of PPARγ].
abstract: OBJECTIVE
  To investigate the effects of leptin on the differentiation of RAW264.7 cells into osteoclasts induced by soluble receptor activator of nuclear factor-κB ligand (sRANKL) and its possible mechanism.
  
  
  METHODS
  The effects of leptin on the differentiation from osteoclast precursor cells into osteoclasts were detected by tartrate-resistant acid phosphatase (TRAP) staining. The mRNA expressions of TRAP and peroxisome proliferator-activated receptor γ (PPARγ) in osteoclast precursor cells were measured by real-time quantitative PCR (qRT-PCR). The protein expressions of TRAP and PPARγ were detected by Western blotting.
  
  
  RESULTS
  Compared with sRANKL treatment group, the differentiation from osteoclast precursor cells into osteoclasts was significantly inhibited in sRANKL combined with leptin treatment groups. Additionally, the mRNA and protein expressions of both TRAP and PPARγ significantly decreased in sRANKL combined with leptin treatment groups compared with the sRANKL treatment alone. Furthermore, the expression of PPARγ was reduced in a dose-dependent manner.
  
  
  CONCLUSIONS
  Leptin could inhibit the differentiation of RAW264.7 cells into osteoclasts via inhibiting the expression of PPARγ.
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Zhengyu
authors: Z
authors: Wang
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Yun
authors: Y
authors: Zhu
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Xiaozhong
authors: X
authors: Yang
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Gang
authors: G
authors: Yang
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Weiwen
authors: W
authors: Zhu
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Tao
authors: T
authors: Ma
authors: Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Jian
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D020738:Leptin / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism
chemical_list: D020738:Leptin; D047495:PPAR gamma