title: Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer.
abstract: Colorectal cancer (CRC) remains an important public health problem in the world. Long noncoding RNA (lncRNA) is an RNA molecular that is longer than 200 nucleotides and cannot be translated into a protein. Recent studies have shown that lncRNAs play important roles in carcinogenesis and cancer metastasis. The aim of this study was to evaluate the expression and biological role of lncRNA maternally expressed gene 3 (MEG3) in colorectal cancer. Quantitative real-time-PCR (qRT-PCR) was performed to investigate the expression of MEG3 in tumor tissues and corresponding nontumor colorectal tissues from 62 patients. The lower expression of MEG3 was remarkably correlated with low histological grade, deep tumor invasion, and advanced tumor node metastasis (TNM) stage. Multivariate analyses revealed that MEG3 expression served as an independent predictor for overall survival. Further experiments revealed that overexpressed MEG3 significantly inhibited CRC cell proliferation both in vitro and in vivo. In conclusion, our study demonstrated that MEG3 is involved in the development and progression of colorectal cancer by regulating cell proliferation and shows that MEG3 may be a potential diagnostic and prognostic target in patients with colorectal cancer.
authors: Cancer Research and Therapy Center, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu, China.
authors: Dan-Dan
authors: DD
authors: Yin
authors: Zhi-Jun
authors: ZJ
authors: Liu
authors: Erbao
authors: E
authors: Zhang
authors: Rong
authors: R
authors: Kong
authors: Zhi-Hong
authors: ZH
authors: Zhang
authors: Ren-Hua
authors: RH
authors: Guo
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C498907:MEG3 non-coding RNA, human; D062085:RNA, Long Noncoding
references: Oncotarget. 2014 Apr 30;5(8):2276-92
references: 24810364
references: J Cell Biochem. 2012 Jun;113(6):1868-74
references: 22234798
references: Med Oncol. 2014 Nov;31(11):253
references: 25326054
references: Oncol Lett. 2014 Aug;8(2):869-875
references: 25013510
references: Cancer Res. 2011 Jun 1;71(11):3852-62
references: 21558391
references: J Clin Endocrinol Metab. 2003 Nov;88(11):5119-26
references: 14602737
references: J Biol Chem. 2007 Aug 24;282(34):24731-42
references: 17569660
references: Oncogene. 2011 Apr 21;30(16):1956-62
references: 21151178
references: CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
references: 19474385
references: Endocrinology. 2010 Mar;151(3):939-47
references: 20032057
references: Front Genet. 2012 Mar 08;3:32
references: 22408643
references: Cancer Res. 2011 Oct 15;71(20):6320-6
references: 21862635
references: J Int Med Res. 2011;39(6):2119-28
references: 22289527
references: Cancer Res. 2012 Mar 1;72(5):1126-36
references: 22258453
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D983-6
references: 23175614
references: Cancer Res. 2014 Mar 15;74(6):1651-60
references: 24473064
references: Br J Cancer. 2014 Aug 12;111(4):736-48
references: 25025966
references: Dev Dyn. 1998 Jun;212(2):214-28
references: 9626496
references: PLoS One. 2012;7(11):e49462
references: 23145177
references: Int J Cancer. 2011 Aug 15;129(4):773-9
references: 21400503
references: J Mol Endocrinol. 2012 Apr 02;48(3):R45-53
references: 22393162
references: Cancer. 2010 Feb 1;116(3):544-73
references: 19998273
references: Clin Cancer Res. 2014 Mar 15;20(6):1531-41
references: 24449823
references: Trends Genet. 2008 Jun;24(6):306-16
references: 18471925
references: Mol Biosyst. 2013 Mar;9(3):407-11
references: 23295831
references: Pathology. 2014 Aug;46(5):396-401
references: 24977734
references: J Neurooncol. 2013 Mar;112(1):1-8
references: 23307326
references: Trends Genet. 2010 Jan;26(1):21-8
references: 19944475
references: Cancer Res. 2011 Jan 1;71(1):3-7
references: 21199792
references: Trends Cell Biol. 2011 Jun;21(6):354-61
references: 21550244
references: Nature. 2010 Apr 15;464(7291):1071-6
references: 20393566