title: Astragalin inhibits IL-1β-induced inflammatory mediators production in human osteoarthritis chondrocyte by inhibiting NF-κB and MAPK activation.
abstract: Astragalin, a bioactive component isolated from Rosa agrestis, has been described to exhibit anti-inflammatory activity. The aim of this study was to investigate the anti-inflammatory effects and the underlying mechanisms of astragalin on IL-1β-stimulated human osteoarthritis chondrocyte. The production of NO and PGE2 was detected by Griess reaction and ELISA. The expression of iNOS and COX-2 was detected by western blotting. The expression of NF-κB and MAPKs was detected by western blot analysis. We found that astragalin dose-dependently inhibited IL-1β-induced NO and PGE2 production, as well as iNOS and COX-2 expression. Meanwhile, western blot analysis showed that astragalin inhibited IL-1β-induced NF-κB and MAPK activation in human osteoarthritis chondrocyte. In addition, astragalin was found to activate PPAR-γ. The inhibition of astragalin on IL-1β-induced NO and PGE2 production can be reversed by PPAR-γ antagonist GW9662. Astragalin suppressed IL-1β-induced inflammatory mediators via activating PPAR-γ, which subsequently inhibited IL-1β-induced NF-κB and MAPK activation. Astragalin may be a potential agent in the treatment of osteoarthritis.
authors: Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People's Republic of China.
authors: Zhiqiang
authors: Z
authors: Ma
authors: Children's Hospital of Harbin, Harbin, Heilongjiang Province 150010, People's Republic of China.
authors: Taikui
authors: T
authors: Piao
authors: Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People's Republic of China.
authors: Yanlong
authors: Y
authors: Wang
authors: Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People's Republic of China. Electronic address: Liujianyu6@hotmail.com.
authors: Jianyu
authors: J
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000813:Anilides / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000276:immunology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology; D044949:Kaempferols / Q000494:pharmacology*; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000276:immunology; D047495:PPAR gamma / Q000037:antagonists & inhibitors; D027826:Rosa / Q000276:immunology*; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D044949:Kaempferols; D016328:NF-kappa B; D047495:PPAR gamma; D009569:Nitric Oxide; C001579:astragalin; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; D015232:Dinoprostone
keywords: Astragalin; IL-1β; NF-κB; Osteoarthritis