title: Alpha-synuclein amyloid oligomers act as multivalent nanoparticles to cause hemifusion in negatively charged vesicles.
abstract: Multivalent membrane binding sites on the α-synuclein oligomer result in clustering of vesicles and hemifusion of negatively charged model membranes. These multivalent, biological nanoparticles are reminiscent of inorganic nanoparticles in their interactions with membranes. Alpha-synuclein oligomers induce lipid exchange efficiently, with fewer than 10 oligomers/vesicle required to complete hemifusion. No full fusion or vesicle content mixing is observed.
authors: Nanobiophysics, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.
authors: Anja N D
authors: AN
authors: Stefanovic
authors: Nanobiophysics, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.|MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.
authors: Mireille M A E
authors: MM
authors: Claessens
authors: Nanobiophysics, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.
authors: Christian
authors: C
authors: Blum
authors: Nanobiophysics, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.|MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, PO Box 217, 7500, AE, Enschede, The Netherlands.|FOM Institute AMOLF, Science Park 104, 1098, XG, Amsterdam, The Netherlands.
authors: Vinod
authors: V
authors: Subramaniam
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D055503:Protein Multimerization; D053835:Unilamellar Liposomes / Q000737:chemistry*; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: D000682:Amyloid; D005456:Fluorescent Dyes; D010715:Phosphatidylglycerols; D053835:Unilamellar Liposomes; D051844:alpha-Synuclein; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol
keywords: Parkinson's disease; alpha-synuclein; hemifusion; multivalent nanoparticles; oligomers; vesicles