title: Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate.
abstract: Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05-1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed.
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. j.pliego.sandoval@zoho.com.
authors: Jorge
authors: J
authors: Pliego
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. jcmateos@ciatej.mx.
authors: Juan Carlos
authors: JC
authors: Mateos
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. jrodriguez@ciatej.mx.
authors: Jorge
authors: J
authors: Rodriguez
authors: Departament d'Enginyeria Química, Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. francisco.valero@uab.cat.
authors: Francisco
authors: F
authors: Valero
authors: Departament de Química, Facultat de Ciències, Edifici C-Nord, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. MariaDelMar.Baeza@uab.cat.
authors: Mireia
authors: M
authors: Baeza
authors: Grupo de Biodinámica y Sistemas Alineales, División de Matemáticas Aplicadas, Instituto Potosinode Investigación Científicay Tecnológica. A.C. Camino a la Presa San José 2055, Lomas 4 Sección, C.P. 78216, San Luis Potosí S.L.P., Mexico. rfemat@ipicyt.edu.mx.
authors: Ricardo
authors: R
authors: Femat
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. rcamacho@ciatej.mx.
authors: Rosa
authors: R
authors: Camacho
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. gsandoval@ciatej.mx.
authors: Georgina
authors: G
authors: Sandoval
authors: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico. eherrera@ciatej.mx.
authors: Enrique J
authors: EJ
authors: Herrera-López
mesh_terms: D015374:Biosensing Techniques; D002087:Butyrates / Q000737:chemistry; D004950:Esterases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D002087:Butyrates; C033592:4-nitrophenyl butyrate; D004950:Esterases; D008049:Lipase
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