title: Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin.
abstract: Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation in a maturation medium on the meiotic competence and fertilisability of sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM199 supplemented with sericin at various concentrations of 0 (control), 0.1, 0.25 and 0.5%, either with or without bovine serum albumin (BSA). When the COCs were matured without BSA, the supplementation of 0.1% sericin significantly increased the rates of maturation to metaphase II and the total fertilisation of oocytes compared with the other concentrations of sericin. When the COCs were matured with BSA, the beneficial effects of 0.1% sericin supplementation on the maturation and fertilisation of oocytes were not observed. Our findings indicate that supplementation with 0.1% sericin during maturation culture may improve the nuclear maturation and fertilisability of sheep oocytes. Moreover, it may be possible to replace BSA with sericin in chemically defined media without the risk of disease transmission.
authors: Bogor Agricultural University Division of Reproduction, Department of Clinic, Reproduction, and Pathology Bogor, Jawa Barat Indonesia.
authors: Cut
authors: C
authors: Yasmin
authors: The United Graduate School of Veterinary Science Laboratory of Animal Reproduction Yamaguchi 753-8515 Japan.
authors: Takeshige
authors: T
authors: Otoi
authors: Bogor Agricultural University Division of Reproduction, Department of Clinic, Reproduction, and Pathology Bogor, Jawa Barat Indonesia.
authors: Mohamad Agus
authors: MA
authors: Setiadi
authors: Bogor Agricultural University Division of Reproduction, Department of Clinic, Reproduction, and Pathology Bogor, Jawa Barat Indonesia.
authors: Ni Wayan Kurniani
authors: NW
authors: Karja
keywords: Albumin; antioxidants; in vitro maturation; meiotic competence; ovine oocyte; sericin; silk protein