title: CXCL13 promotes the effect of bone marrow mesenchymal stem cells (MSCs) on tendon-bone healing in rats and in C3HIOT1/2 cells.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) are potential effective therapy for tissue repair and bone regeneration. In present study, the effects of CXC chemokine ligand-13 (CXCL13) were evaluated on tendon-bone healing of rats.
  
  
  METHODS
  Tendon bone healing of the rat model was established and biomechanical testing was performed at 2, 4, 8 weeks after surgery. Murine mesenchymal cell line (C3HIOT1/2 cells) was cultured. The expression of miRNA-23a was detected by real-time PCR. The protein expression of ERK1/2, JNK and p38 was detected by western blotting. MiR-23a mimic and inhibitor were used to overexpress or silence the expression of miR-23a.
  
  
  RESULTS
  MSCs significantly elevated the levels of ultimate load to failure, stiffness and stress in specimens of rats, the effects of which were enhanced by CXCL13. The expression of miR-23a was down-regulated and the protein of ERK1/2 level was up-regulated by CXCL13 treatment in both in vivo and in vitro experiments. ERK1/2 expression was elevated by overexpression of miR-23a and reduced by miR-23a inhibitor.
  
  
  CONCLUSIONS
  These findings revealed that CXCL13 promoted the tendon-bone healing in rats with MSCs treatment, and implied that the activation of ERK1/2 via miR-23a was involved in the process of MSCs treated bone regeneration.
authors: Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. ftiangogo@163.com.
authors: Feng
authors: F
authors: Tian
authors: Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Xianglu_Ji@163.com.
authors: Xiang-Lu
authors: XL
authors: Ji
authors: Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Wan_anXiao@163.com.
authors: Wan-An
authors: WA
authors: Xiao
authors: Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Bin_Wang1011@163.com.
authors: Bin
authors: B
authors: Wang
authors: Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Fei_Wang1001@163.com.
authors: Fei
authors: F
authors: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D054382:Chemokine CXCL13 / Q000378:metabolism* / Q000494:pharmacology; D004195:Disease Models, Animal; D015870:Gene Expression; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference; D051381:Rats; D013708:Tendon Injuries / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D014945:Wound Healing
chemical_list: D054382:Chemokine CXCL13; D035683:MicroRNAs; D020928:Mitogen-Activated Protein Kinases; C000590974:MIRN23a microRNA, rat
references: Tissue Eng Part A. 2009 Nov;15(11):3487-97
references: 19438323
references: Nucleic Acids Res. 2014 Aug;42(14):9313-26
references: 25030899
references: J Korean Med Sci. 2009 Aug;24(4):547-54
references: 19654931
references: Nat Biotechnol. 2009 May;27(5):459-61
references: 19363475
references: Cell Commun Adhes. 2004 Sep-Dec;11(5-6):137-53
references: 16194881
references: J Bone Miner Res. 2006 Jul;21(7):993-1002
references: 16813520
references: Exp Gerontol. 2004 Apr;39(4):659-65
references: 15050303
references: J Biol Chem. 2000 Mar 31;275(13):9645-52
references: 10734116
references: PLoS One. 2012;7(7):e40831
references: 22808271
references: PLoS One. 2013;8(9):e75697
references: 24086616
references: Stem Cells. 2013 Dec;31(12):2703-13
references: 23836581
references: Acta Pharmacol Sin. 2013 Jun;34(6):747-54
references: 23736003
references: Bone. 2008 Jul;43(1):40-7
references: 18486585
references: Nature. 2001 Mar 1;410(6824):37-40
references: 11242034
references: Int Orthop. 2012 May;36(5):1099-107
references: 22134708
references: Cancer Lett. 2009 Sep 28;283(1):29-35
references: 19375853
references: FASEB J. 2013 Sep;27(9):3505-13
references: 23704087
references: Exp Cell Res. 2008 May 1;314(8):1777-88
references: 18378228
references: Exp Mol Pathol. 2014 Aug;97(1):23-30
references: 24269648
references: Am J Sports Med. 2014 Aug;42(8):1920-9
references: 24853168
references: Lab Invest. 2014 Mar;94(3):286-96
references: 24395111
references: Stem Cells Int. 2013;2013:132642
references: 23554816
references: J Mol Cell Biol. 2011 Apr;3(2):123-31
references: 21278199
references: Int J Biol Sci. 2011;7(6):762-8
references: 21698002
references: Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9863-8
references: 21628588
references: J Biol Chem. 2007 Oct 19;282(42):30938-48
references: 17720811