title: Probiotic and technological properties of Lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis.
abstract: This work characterizes a set of lactobacilli strains isolated from the stomach of healthy humans that might serve as probiotic cultures. Ten different strains were recognized by rep-PCR and PFGE fingerprinting among 19 isolates from gastric biopsies and stomach juice samples. These strains belonged to five species, Lactobacillus gasseri (3), Lactobacillus reuteri (2), Lactobacillus vaginalis (2), Lactobacillus fermentum (2) and Lactobacillus casei (1). All ten strains were subjected to a series of in vitro tests to assess their functional and technological properties, including acid resistance, bile tolerance, adhesion to epithelial gastric cells, production of antimicrobial compounds, inhibition of Helicobacter pylori, antioxidative activity, antibiotic resistance, carbohydrate fermentation, glycosidic activities, and ability to grow in milk. As expected, given their origin, all strains showed good resistance to low pH (3.0), with small reductions in counts after 90 min exposition to this pH. Species- and strain-specific differences were detected in terms of the production of antimicrobials, antagonistic effects toward H. pylori, antioxidative activity and adhesion to gastric epithelial cells. None of the strains showed atypical resistance to a series of 16 antibiotics of clinical and veterinary importance. Two L. reuteri strains were deemed as the most appropriate candidates to be used as potential probiotics against microbial gastric disorders; these showed good survival under gastrointestinal conditions reproduced in vitro, along with strong anti-Helicobacter and antioxidative activities. The two L. reuteri strains further displayed appropriated technological traits for their inclusion as adjunct functional cultures in fermented dairy products.
authors: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC) Villaviciosa, Spain.
authors: Susana
authors: S
authors: Delgado
authors: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC) Villaviciosa, Spain.
authors: Analy M O
authors: AM
authors: Leite
authors: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC) Villaviciosa, Spain.
authors: Patricia
authors: P
authors: Ruas-Madiedo
authors: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC) Villaviciosa, Spain.
authors: Baltasar
authors: B
authors: Mayo
keywords: anti-Helicobacter activity; antioxidative activity; fermentation capability; functional characterization; gastric lactobacilli; specific probiotics; stomach microbiota
references: J Clin Microbiol. 1999 Sep;37(9):3062-4
references: 10449509
references: J Antimicrob Chemother. 2008 Apr;61(4):831-4
references: 18263567
references: Gut. 2007 Jun;56(6):772-81
references: 17170018
references: Genome Res. 1995 Nov;5(4):408-18
references: 8750201
references: BMC Microbiol. 2008 Jan 29;8:22
references: 18230145
references: Appl Environ Microbiol. 2004 Jan;70(1):518-26
references: 14711683
references: J Appl Microbiol. 1998 May;84(5):759-68
references: 9674129
references: PLoS One. 2008 Jul 30;3(7):e2836
references: 18665274
references: J Antimicrob Chemother. 2008 Jan;61(1):139-42
references: 17965421
references: Dig Dis Sci. 2004 Aug;49(7-8):1095-102
references: 15387328
references: J Food Sci. 2007 Oct;72(8):M310-5
references: 17995611
references: Appl Environ Microbiol. 2005 Dec;71(12):8982-6
references: 16332905
references: Int J Food Microbiol. 2005 Oct 25;104(3):267-77
references: 15975679
references: Appl Microbiol Biotechnol. 2004 Apr;64(3):326-32
references: 14735324
references: Appl Environ Microbiol. 2010 Jan;76(2):400-5
references: 19948869
references: J Antimicrob Chemother. 1999 Nov;44(5):629-40
references: 10552979
references: Helicobacter. 2008 Oct;13 Suppl 1:1-6
references: 18783514
references: Int J Food Microbiol. 1998 May 5;41(1):45-51
references: 9631336
references: PLoS One. 2009 Nov 24;4(11):e7985
references: 19956741
references: Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):732-7
references: 16407106
references: World J Gastroenterol. 2010 Jan 28;16(4):445-52
references: 20101769
references: Microb Ecol. 2013 Apr;65(3):763-72
references: 23397369
references: J Mol Microbiol Biotechnol. 2007;13(4):194-9
references: 17827969
references: Helicobacter. 2008 Apr;13(2):127-34
references: 18321302
references: J Clin Gastroenterol. 2005 Sep;39(8):692-8
references: 16082279
references: J Appl Microbiol. 2006 Jun;100(6):1324-32
references: 16696680
references: Lett Appl Microbiol. 2008 Oct;47(4):269-74
references: 19241519
references: Lett Appl Microbiol. 2009 Jun;48(6):797-800
references: 19344357
references: Int J Food Microbiol. 2002 Feb 5;72(3):215-24
references: 11845820
references: Nat Rev Cancer. 2002 Jan;2(1):28-37
references: 11902583
references: Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):77-82
references: 15653856
references: J Food Prot. 2001 Jun;64(6):833-7
references: 11403134