title: Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations.
abstract: The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono-, di-, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.
authors: Faculty of Chemical, Environmental and Biological Science and Technology, Analytical Center, Dalian University of Technology, Dalian, China.
authors: Huihui
authors: H
authors: Wan
authors: Qianying
authors: Q
authors: Sheng
authors: Hongmin
authors: H
authors: Zhong
authors: Xiujie
authors: X
authors: Guo
authors: Qing
authors: Q
authors: Fu
authors: Yanfang
authors: Y
authors: Liu
authors: Xingya
authors: X
authors: Xue
authors: Xinmiao
authors: X
authors: Liang
mesh_terms: D002021:Buffers; D002241:Carbohydrates / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D045786:Diterpenes, Kaurane / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009584:Nitrogen / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D012503:Saponins / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D002021:Buffers; D002241:Carbohydrates; D045786:Diterpenes, Kaurane; D005960:Glucosides; D006027:Glycosides; D009844:Oligosaccharides; D012492:Salts; D012503:Saponins; C012042:steviol; D012822:Silicon Dioxide; D009584:Nitrogen; D012825:Silicon
keywords: Carbohydrates; Hydrophilic interaction liquid chromatography; Silica; Silicon oxynitride; Stationary phases