title: Mitochondrial coenzyme Q10 determination via isotope dilution liquid chromatography tandem mass spectrometry.
abstract: Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain. Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ10 in isolated mitochondria. In the assay, mitochondrial suspensions are spiked with CoQ10-[(2)H6] internal standard, extracted with organic solvents, and CoQ10 quantified by LC-MS/MS using multiple reaction monitoring (MRM).
authors: Laboratory Division HUSLAB, Helsinki University Central Hospital, Haartmaninkatu 2, Helsinki, 00029, Finland, outi.itkonen@hus.fi.
authors: Outi
authors: O
authors: Itkonen
authors: Ursula
authors: U
authors: Turpeinen
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D007553:Isotope Labeling; D008928:Mitochondria / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10