title: Combined use of high-resolution α-glucosidase inhibition profiling and high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for investigation of antidiabetic principles in crude plant extracts.
abstract: Type 2 diabetes is a metabolic disorder affecting millions of people worldwide, and new drug leads or functional foods containing selective α-glucosidase inhibitors are needed. Crude extract of 24 plants were assessed for α-glucosidase inhibitory activity. Methanol extracts of Cinnamomum zeylanicum bark, Rheum rhabarbarum peel, and Rheum palmatum root and ethyl acetate extracts of C. zeylanicum bark, Allium ascalonicum peel, and R. palmatum root showed IC50 values below 20 μg/mL. Subsequently, high-resolution α-glucosidase profiling was used in combination with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for identification of metabolites responsible for the α-glucosidase inhibitory activity. Quercetin (1) and its dimer (2), trimer (3), and tetramer (4) were identified as main α-glucosidase inhibitors in A. ascalonicum peel, whereas (E)-piceatannol 3'-O-β-D-glucopyranoside (5), (E)-rhapontigenin 3'-O-β-D-glucopyranoside (6), (E)-piceatannol (8), and emodin (12) were identified as main α-glucosidase inhibitors in R. palmatum root.
authors: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors: Kenneth T
authors: KT
authors: Kongstad
authors: Ceylan
authors: C
authors: Özdemir
authors: Asmah
authors: A
authors: Barzak
authors: Sileshi G
authors: SG
authors: Wubshet
authors: Dan
authors: D
authors: Staerk
mesh_terms: D027424:Cinnamomum / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D012250:Rheum / Q000737:chemistry*; D027781:Shallots / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D000520:alpha-Glucosidases / Q000032:analysis
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000520:alpha-Glucosidases
keywords: Allium ascalonicum; Cinnamonum zeylanicum; HPLC−SPE−NMR; Rheum palmatum; high-resolution screening; type 2 diabetes; α-glucosidase