title: Y-box Binding Protein-1 Is Part of a Complex Molecular Network Linking ΔNp63α to the PI3K/akt Pathway in Cutaneous Squamous Cell Carcinoma.
abstract: Cutaneous squamous cell carcinomas (SCCs) typically lack somatic oncogene-activating mutations and most of them contain p53 mutations. However, the presence of p53 mutations in skin premalignant lesions suggests that these represent early events during tumor progression and additional alterations may be required for SCC development. SCC cells frequently express high levels of ΔNp63α and Y-box binding 1 (YB-1 or YBX1) oncoproteins. Here, we show that knockdown of YB-1 in spontaneously immortalized HaCaT and non-metastatic SCC011 cells led to a dramatic decrease of ΔNp63α, cell detachment and death. In highly metastatic SCC022 cells, instead, YB-1 silencing induces PI3K/AKT signaling hyperactivation which counteracts the effect of YB-1 depletion and promotes cell survival. In summary, our results unveil a functional cross-talk between YB-1, ΔNp63α and the PI3K/AKT pathway critically governing survival of squamous carcinoma cells.
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Annaelena
authors: A
authors: Troiano
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Irene Schiano
authors: IS
authors: Lomoriello
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Orsola
authors: O
authors: di Martino
authors: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors: Sabato
authors: S
authors: Fusco
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Alessandra
authors: A
authors: Pollice
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Maria
authors: M
authors: Vivo
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Girolama
authors: G
authors: La Mantia
authors: Department of Biology, University of Naples 'Federico II,', Naples, Italy.
authors: Viola
authors: V
authors: Calabrò
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D051058:Oncogene Protein v-akt / Q000235:genetics* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D051840:Y-Box-Binding Protein 1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C115051:TP63 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D051840:Y-Box-Binding Protein 1; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt