title: Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection.
abstract: BACKGROUND
  Epigallocatechin-3-gallate (EGCG) is a flavonoid extracted from green tea that demonstrated antimicrobial activity.
  
  
  OBJECTIVE
  To evaluate the efficacy of EGCG 0.5%, 1%, and 2% concentrations as an antimicrobial solution in dentin caries-like lesions induced in a bacterial-based in vitro model.
  
  
  METHODS
  Twenty-five human dentin specimens were submitted to a microbial-based caries model by immersion in brain heart infusion (BHI) broth inoculated with Streptococcus mutans UA159, for 5 days. After the demineralization period, the specimens were randomly divided into groups: Group I: 0.9% saline solution; Group II: 2% chlorhexidine digluconate; Group III: 0.5% EGCG; Group IV: 1% EGCG; and Group V: 2% EGCG. After the treatments, carious dentin samples were harvested from dentin specimens and analyzed by colony-forming unit (CFU) counts. Data were analyzed by ANOVA and Tukey's test.
  
  
  RESULTS
  Log reduction values (SD, CFU.mg(-1)) for Groups I-V were: 5.02 (0.16), 3.96 (0.43), 4.74 (0.26), 4.89 (0.56), and 4.91 (0.40), respectively. There was no statistical difference between the EGCG concentrations and saline solution (P > 0.05). Furthermore, there was no statistical difference between EGCG concentrations (P > 0.05). However, there was a statistically significant difference between the chlorhexidine digluconate group and the other groups (P < 0.05).
  
  
  CONCLUSIONS
  EGCG at the studied concentrations were not effective in eliminating S. mutans from dentin caries-like lesions.
authors: Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Jorgiana Silva
authors: JS
authors: de Assis
authors: Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Ramille Araujio
authors: RA
authors: Lima
authors: Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Juliana Paiva
authors: JP
authors: Marques Lima
authors: Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Lidiany Karle
authors: LK
authors: Azevedo Rodrigues
authors: Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Sérgio Lima
authors: SL
authors: Santiago
keywords: Dentin; catechin; streptococcus mutans
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