title: Depletion of cellular adenosine triphosphate and hepatocellular damage in rat after subchronic exposure to leachate from anthropogenic recycling site.
abstract: One of the major hazards arising from recycling sites is the generation of leachate containing mixed metal. This study evaluated the toxic effects of leachate obtained from Elewi Odo municipal auto-battery recycling site (EOMABRSL) on male liver functions using hepatic indices and biomarker of cellular adenosine triphosphate (ATP) in rat via the oral route. Concentrations of heavy metals analysis showed that lead, cadmium, nickel, chromium, manganese, and iron were 1.5-, 2-, 2.5-, 1.36-, 19.61-, and 8.89-folds, respectively, higher than acceptable limits set by regulatory authority World Health Organization. Copper, zinc, and cobalt were 5.9-, 300-, and 1.02-folds, respectively, lower than permissible limits. The EOMABRSL was administered at 20, 40, 60, 80, and 100% concentrations to adult male rats for 60 days. Following exposure, plasma and livers were collected for several biochemistry assays. Exposure of animals to EOMABRSL resulted in 27.51, 28.14, 63.93, 28.42, and 40.16% increase in aspartate aminotransferase activity, whereas it elevated alanine aminotransferase activity by 5.35, 22.33, 88.68, 183.02, and 193.08%, respectively, when compared with the control. Similarly, γ-glutamyl transferase activity increased by 111.22, 114.19, 122.96, 573.14, and 437.02%, respectively, when compared with the control. EOMABRSL administration significantly decreased catalase activity and reduced glutathione level and superoxide dismutase with concomitant increase in malondialdehyde and hydrogen peroxide levels. Also, significant (p < 0.05) decrease in lactate dehydrogenase (LDH) activity (marker of cellular ATP) was observed. Taken together, the hepatotoxicity of EOMABRSL could be due to the depletion of LDH and induction of oxidative damage, which may suggest possible health hazards in subjects with occupational or environmental exposure.
authors: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria Department of Biosciences and Biotechnology, Biochemistry Unit, College of Pure and Applied Sciences, Kwara State University, Malete, Nigeria akintundejacob@yahoo.com.
authors: J K
authors: JK
authors: Akintunde
authors: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
authors: G
authors: G
authors: Oboh
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity*; D009549:Nigeria; D017208:Rats, Wistar; D059027:Recycling; D059550:Toxicity Tests, Subchronic; D018505:Waste Management / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Cellular ATP; anthropogenic recycling site; hepatocellular damage; mixed metal; rat model