title: In vitro antimicrobial activity of extracts and compounds isolated from Cladonia uncialis.
abstract: Heptane (Hep), diethyl ether (Et2O), acetone (Me2CO) and methanolic (MeOH) extracts, as well as (-)-usnic acid and squamatic acid, were obtained from thallus of Cladonia uncialis (Cladoniaceae). The antimicrobial activities of these extracts, (-)-usnic acid and squamatic acid, were tested against reference strains: Staphylococcus aureus, Escherichia coli and Candida albicans. In addition, Me2CO extract was analysed against 10 strains of Methicillin-resistant S. aureus (MRSA) isolated from patients. All extracts exerted antibacterial activity against the reference strain S. aureus, comparably to chloramphenicol [minimum inhibitory concentration (MIC) = 5.0 μg/mL]. The Me2CO extract exhibited the strongest activity against S. aureus (MIC = 0.5 μg/mL), higher than (-)-usnic acid, whereas squamatic acid proved inactive. The Me2CO extract showed potent antimicrobial activity against MRSA (MIC 2.5-7.5 μg/mL). Also no activity of C. uncialis extracts against E. coli and C. albicans was observed.
authors: a Department of Pharmacognosy , Poznan University of Medical Sciences , Święcickiego 4, 60-781 Poznań , Poland.
authors: Elżbieta
authors: E
authors: Studzińska-Sroka
authors: b Institute of Natural Fibres and Medicinal Plants , Libelta 27, 61-707 Poznań , Poland.
authors: Elżbieta
authors: E
authors: Hołderna-Kędzia
authors: c Department of Pharmacognosy , Jagiellonian University Medical College , Medyczna 9, 30-688 Kraków , Poland.
authors: Agnieszka
authors: A
authors: Galanty
authors: a Department of Pharmacognosy , Poznan University of Medical Sciences , Święcickiego 4, 60-781 Poznań , Poland.
authors: Wiesława
authors: W
authors: Bylka
authors: d Faculty of Chemistry, Adam Mickiewicz University , Grunwaldzka 6, 60-780 Poznań , Poland.
authors: Karol
authors: K
authors: Kacprzak
authors: e Department of Laboratory Diagnostics , Josef Strus Multidisciplinary Municipal Hospital , Szwajcarska 3, 61-285 Poznań , Poland.
authors: Karolina
authors: K
authors: Ćwiklińska
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D001572:Benzofurans / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008011:Lichens / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D001572:Benzofurans; C073339:usnic acid
keywords: Cladonia uncialis; antimicrobial activity; extracts; squamatic acid; usnic acid