title: Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.
abstract: The adenovirus E4orf4 protein expressed at high levels kills cancer cells but not normal human primary cells. Previous studies suggested that disruption of processes that regulate mitosis may underlie E4orf4 toxicity. Here we have used live imaging to show that E4orf4 induces a slowed defective transit through mitosis, exhibiting a delay or often failure in cytokinesis that may account for an accumulation of G1 tetraploids in the population of dying E4orf4-expressing cells.
authors: Department of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.
authors: Neera
authors: N
authors: Sriskandarajah
authors: Department of Biochemistry, McGill University, Montreal, Québec, Canada.
authors: Paola
authors: P
authors: Blanchette
authors: Department of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.
authors: Thomas J
authors: TJ
authors: Kucharski
authors: Department of Biochemistry, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada.
authors: Jose G
authors: http://orcid.org/0000-0002-3713-6839
authors: JG
authors: Teodoro
authors: Department of Biochemistry, McGill University, Montreal, Québec, Canada Department of Oncology, McGill University, Montreal, Québec, Canada Goodman Cancer Research Centre, McGill University, Montreal, Québec, Canada philip.branton@mcgill.ca.
authors: Philip E
authors: PE
authors: Branton
mesh_terms: D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D057054:Molecular Imaging / Q000379:methods*; D059008:Time-Lapse Imaging; D014764:Viral Proteins / Q000502:physiology* / Q000648:ultrastructure*
chemical_list: C121517:E4orf4 protein, adenovirus; D014764:Viral Proteins
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