title: Go-sha-jinki-Gan (GJG), a traditional Japanese herbal medicine, protects against sarcopenia in senescence-accelerated mice.
abstract: Sarcopenia is characterized by age-associated skeletal muscle atrophy and reduced muscle strength; currently, no pharmaceutical treatment is available. Go-sha-jinki-Gan (GJG) is a traditional Japanese herbal medicine that is used to alleviate various age-related symptoms, especially motor disorders. Here, we investigated the effect of GJG on aging-associated skeletal muscle atrophy by using senescence-accelerated mice (SAMP8). Immunohistochemical and western blotting analyses clearly showed that GJG significantly reduced the loss of skeletal muscle mass and ameliorated the increase in slow skeletal muscle fibers in SAMP8 mice compared to control mice. The expression levels of Akt and GSK-3β, the phosphorylation of FoxO4, and the phosphorylations of AMPK and mitochondrial-related transcription factors such as PGC-1α were suppressed, while the expression of MuRF1 increased in SAMP8 mice, but approximated that in senescence-accelerated aging-resistant (SAMR1) mice after GJG treatment. We demonstrate for the first time that GJG has a therapeutic effect against sarcopenia.
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Yuki
authors: Y
authors: Kishida
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Syota
authors: S
authors: Kagawa
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Junsuke
authors: J
authors: Arimitsu
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Miho
authors: M
authors: Nakanishi
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Noriko
authors: N
authors: Sakashita
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Shizue
authors: S
authors: Otsuka
authors: Department of Orthopaedics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Hideki
authors: H
authors: Yoshikawa
authors: Department of Kampo Medicine, Osaka University Graduate School of Medicine, Osaka, Japan. Electronic address: hagihara.keisuke@gmail.com.
authors: Keisuke
authors: K
authors: Hagihara
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000375:Aging; D000818:Animals; D018797:Cell Cycle Proteins; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D009124:Muscle Proteins / Q000378:metabolism; D053580:Muscle Strength / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects*; D009133:Muscular Atrophy / Q000188:drug therapy; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D055948:Sarcopenia / Q000188:drug therapy*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C525394:FoxO4 protein, mouse; D009124:Muscle Proteins; D000070584:Tripartite Motif Proteins; C119642:gosha-jinki-gan; C519530:insulin-like growth factor-1, mouse; D007334:Insulin-Like Growth Factor I; C499084:Trim63 protein, mouse; D044767:Ubiquitin-Protein Ligases; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; D055372:AMP-Activated Protein Kinases
keywords: Go-sha-jinki-Gan (GJG); Sarcopenia; Senescence-accelerated mice (SAMP8)