title: 4-Methylcatechol-induced cell damage in TM4 Sertoli cells.
abstract: 4-Methylcatechol (4-MC) is one of the metabolites of quercetin, which is a potential drug for neuroprotection and tumorigenesis inhibition. This study was performed to investigate the cytotoxic effect of 4-MC in mouse TM4 Sertoli cells. TM4 Sertoli cell viability was significantly inhibited by 4-MC in a time- and dose-dependent manner. The number of apoptotic and dead cells was significantly increased after 4-MC treatment. Caspase 3 activity increased by prolonged exposure of TM4 Sertoli cells to 200 μM 4-MC. The 4-MC significantly upregulated the mRNA level of Bax gene and considerably downregulated the Bcl-2 gene expression in a concentration-dependent manner. Results showed that 4-MC could induce TM4 Sertoli cell apoptosis, and the cytotoxic effect of 4-MC on TM4 Sertoli cells may be associated with upregulated Bax gene expression, which induced caspase cascade activation.
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Chunjin
authors: C
authors: Li
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Wanhong
authors: W
authors: Li
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Shuxiong
authors: S
authors: Chen
authors: Department of Rehabilitation, The second hospital of Jilin University, Changchun, 130041, P. R. China.
authors: Zhichen
authors: Z
authors: Kang
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Lina
authors: L
authors: Sun
authors: Hongjiao
authors: H
authors: Li
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Lu
authors: L
authors: Chen
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Jiahui
authors: J
authors: Rao
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Yun
authors: Y
authors: Zhao
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Jiaxin
authors: J
authors: Yu
authors: College of Animal Sciences, Jilin University, 5333 Xi'an Avenue, Changchun, Jilin Province, 130062, P.R. China.
authors: Xu
authors: X
authors: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D012708:Sertoli Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology
chemical_list: D002396:Catechols; C018599:4-methylcatechol; D053148:Caspase 3
keywords: 4-Methylcatechol; TM4 Sertoli cells; apoptosis; cytotoxicity