title: Arabidopsis HFR1 is a potential nuclear substrate regulated by the Xanthomonas type III effector XopD(Xcc8004).
abstract: XopDXcc8004, a type III effector of Xanthomonas campestris pv. campestris (Xcc) 8004, is considered a shorter version of the XopD, which lacks the N-terminal domain. To understand the functions of XopDXcc8004, in planta, a transgenic approach combined with inducible promoter to analyze the effects of XopDXcc8004 in Arabidopsis was done. Here, the expression of XopDXcc8004, in Arabidopsis elicited the accumulation of host defense-response genes. These molecular changes were dependent on salicylic acid and correlated with lesion-mimic phenotypes observed in XVE::XopDXcc8004 transgenic plants. Moreover, XopDXcc8004 was able to desumoylate HFR1, a basic helix-loop-helix transcription factor involved in photomorphogenesis, through SUMO protease activity. Interestingly, the hfr1-201 mutant increased the expression of host defense-response genes and displayed a resistance phenotype to Xcc8004. These data suggest that HFR1 is involved in plant innate immunity and is potentially regulated by XopDXcc8004.
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan.
authors: Choon Meng
authors: CM
authors: Tan
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
authors: Meng-Ying
authors: MY
authors: Li
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
authors: Pei-Yun
authors: PY
authors: Yang
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
authors: Shu Heng
authors: SH
authors: Chang
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
authors: Yi-Ping
authors: YP
authors: Ho
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
authors: Hong
authors: H
authors: Lin
authors: Department of Plant Pathology, National ChungHsing University, Taichung, Taiwan.
authors: Wen-Ling
authors: WL
authors: Deng
authors: Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan; Institute of Biotechnology, National ChungHsing University, Taichung, Taiwan; NCHU-UCD Plant and Food Biotechnology Center, National ChungHsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National ChungHsing University, Taichung, Taiwan.
authors: Jun-Yi
authors: JY
authors: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology; D020156:Salicylic Acid / Q000276:immunology; D025841:Small Ubiquitin-Related Modifier Proteins / Q000276:immunology; D016959:Xanthomonas campestris / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020156:Salicylic Acid; D025841:Small Ubiquitin-Related Modifier Proteins; C414438:At1g02340 protein, Arabidopsis
references: FEBS Lett. 2010 Jul 2;584(13):2748-58
references: 20493189
references: Mol Cells. 2013 Jan;35(1):70-8
references: 23269432
references: New Phytol. 2014 Nov;204(3):595-608
references: 25040905
references: Nat Rev Genet. 2010 Aug;11(8):539-48
references: 20585331
references: Plant Cell. 2010 Jun;22(6):1998-2016
references: 20525853
references: Methods Mol Biol. 2011;678:65-76
references: 20931373
references: PLoS One. 2010;5(12):e15773
references: 21203472
references: Genome Biol. 2010;11(10):R106
references: 20979621
references: Cell Host Microbe. 2013 Feb 13;13(2):143-54
references: 23414755
references: Mol Plant Microbe Interact. 2013 Apr;26(4):419-30
references: 23252460
references: Microb Biotechnol. 2013 May;6(3):230-40
references: 23433088
references: J Biol Chem. 2013 Jun 28;288(26):19288-95
references: 23673667
references: Annu Rev Phytopathol. 2013;51:473-98
references: 23725467
references: Plant Signal Behav. 2010 Dec;5(12):1597-601
references: 21150289
references: Nat Commun. 2011;2:400
references: 21772271
references: Mol Plant Pathol. 2011 Oct;12(8):715-30
references: 21726373
references: Plant Cell. 2011 Sep;23(9):3498-511
references: 21917550
references: Plant J. 2000 Oct;24(2):265-73
references: 11069700
references: Plant J. 2002 Jun;30(6):711-9
references: 12061902
references: Methods Enzymol. 2002;350:87-96
references: 12073338
references: Plant J. 2002 Jul;31(1):87-95
references: 12100485
references: Mol Microbiol. 2003 Oct;50(2):377-89
references: 14617166
references: Curr Opin Plant Biol. 2004 Aug;7(4):384-90
references: 15231260
references: Gene. 1994 Jul 22;145(1):69-73
references: 8045426
references: Biochem Mol Biol Int. 1994 Sep;34(2):409-17
references: 7849652
references: Plant J. 1998 Dec;16(6):735-43
references: 10069079
references: Curr Biol. 2004 Dec 29;14(24):2296-301
references: 15620659
references: Mol Cell Biol. 2005 Feb;25(4):1549-59
references: 15684403
references: Genes Dev. 2005 Mar 1;19(5):593-602
references: 15741320
references: Plant Cell. 2005 Mar;17(3):804-21
references: 15705947
references: Curr Opin Plant Biol. 2006 Aug;9(4):376-82
references: 16713730
references: Plant Physiol. 2006 Sep;142(1):318-32
references: 16920872
references: Methods Mol Biol. 2006;343:43-53
references: 16988332
references: Annu Rev Microbiol. 2006;60:425-49
references: 16753033
references: Mol Cell. 2006 Nov 3;24(3):341-54
references: 17081986
references: Plant Cell. 2006 Nov;18(11):2919-28
references: 17114357
references: Plant J. 2007 Jan;49(1):79-90
references: 17163880
references: Annu Rev Phytopathol. 2008;46:189-215
references: 18422429
references: Mol Plant Pathol. 2008 Jul;9(4):545-60
references: 18705867
references: Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4935-40
references: 19251652
references: Plant Cell Physiol. 2009 Jun;50(6):1049-61
references: 19376783
references: Plant Signal Behav. 2009 Aug;4(8):713-7
references: 19820324
references: Mol Cells. 2011 Oct;32(4):305-16
references: 21912873
references: J Exp Bot. 2012 Feb;63(4):1619-36
references: 22282535
references: PLoS One. 2012;7(8):e43137
references: 22905217
references: Plant Physiol. 2012 Sep;160(1):145-55
references: 22802612
references: Plant J. 2012 Oct;72(2):345-54
references: 22631228
references: Trends Plant Sci. 2012 Nov;17(11):644-55
references: 22796464
references: Plant Physiol. 2009 Dec;151(4):1930-42
references: 19837819