title: Bioconjugated persistent luminescence nanoparticles for Föster resonance energy transfer immunoassay of prostate specific antigen in serum and cell extracts without in situ excitation.
abstract: A novel Föster resonance energy transfer (FRET) immunoassay based on persistent luminescence nanoparticles (PLNP) was established for prostate specific antigen (PSA) detection in serum and cell extracts without in situ excitation. The specific FRET behavior allows highly selective and sensitive ratiometric photoluminescent detection of PSA in biological samples.
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 30071, China. xpyan@nankai.edu.cn.
authors: Bo-Yue
authors: BY
authors: Wu
authors: Xiu-Ping
authors: XP
authors: Yan
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002457:Cell Extracts; D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D049449:Luminescence; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017430:Prostate-Specific Antigen / Q000097:blood / Q000276:immunology / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D005456:Fluorescent Dyes; D012235:Rhodamines; D017430:Prostate-Specific Antigen; C029773:rhodamine B