title: Aerobic Microbial Community of Insectary Population of Phlebotomus papatasi.
abstract: BACKGROUND
  Microbes particularly bacteria presenting in the gut of haematophagous insects may have an important role in the epidemiology of human infectious disease.
  
  
  METHODS
  The microbial flora of gut and surrounding environmental of a laboratory strain of Phlebotomus papatasi, the main vector of Zoonotic Cutaneous Leishmaniasis (ZCL) in the old world, was investigated. Biochemical reactions and 16s rDNA sequencing of the isolated bacteria against 24 sugars and amino acids were used for bacteria species identification. Common mycological media used for fungi identification as well.
  
  
  RESULTS
  Most isolates belonged to the Enterobacteriaceae, a large, heterogeneous group of gram-negative rods whose natural habitat is the intestinal tract of humans and animals. Enterobacteriaceae groups included Edwardsiella, Enterobacter, Escherichia, Klebsiella, Kluyvera, Leminorella, Pantoea, Proteus, Providencia, Rahnella, Serratia, Shigella, Tatumella, and Yersinia and non Enterobacteriaceae groups included Bacillus, Staphylococcus and Pseudomonas. The most prevalent isolates were Proteus mirabilis and P. vulgaris. These saprophytic and swarming motile bacteria were isolated from all immature, pupae, and mature fed or unfed male or female sand flies as well as from larval and adult food sources. Five fungi species were also isolated from sand flies, their food sources and colonization materials where Candida sp. was common in all mentioned sources.
  
  
  CONCLUSIONS
  Midgut microbiota are increasingly seen as an important factor for modulating vector competence in insect vectors so their possible effects of the mirobiota on the biology of P. papatasi and their roles in the sandfly-Leishmania interaction are discussed.
authors: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Naseh
authors: N
authors: Maleki-Ravasan
authors: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Oshaghi
authors: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Sara
authors: S
authors: Hajikhani
authors: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Zahra
authors: Z
authors: Saeidi
authors: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Amir Ahmad
authors: AA
authors: Akhavan
authors: Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohsen
authors: M
authors: Gerami-Shoar
authors: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Hasan
authors: MH
authors: Shirazi
authors: Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors: Bagher
authors: B
authors: Yakhchali
authors: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Yavar
authors: Y
authors: Rassi
authors: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Davoud
authors: D
authors: Afshar
keywords: Bacteria; Fungi; Leishmaniasis; Microflora; Phlebotomus papatasi; Symbiont
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