title: The association between environmental exposure to pyrethroids and sperm aneuploidy.
abstract: The aim of the present study is to determine whether the environmental exposure to pyrethroids was associated with males sperm chromosome disomy. The study population consisted of 195 men who attended the infertility clinic for diagnostic purposes and who had normal semen concentration of 20-300×10(6) mL(-1) or slight oligozoospermia (semen concentration of 15-20×10(6) mL(-1)) (WHO, 1999). Participants were interviewed and provided a semen sample. The pyrethroids metabolites: 3-phenoxybenzoic acid (3PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (CDCCA), trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (TDCCA) and cis-2,2-dibromovinyl-2,2-dimethylcyclopropane-1-carboxylic acid (DBCA) were analysed in the urine using a validated gas chromatography ion-tap mass spectrometry method. Sperm aneuploidy was assessed using multicolor FISH (DNA probes specific for chromosomes X, Y, 18, 13, 21). Our results showed that CDCCA >50th percentile was associated with disomy of chromosome 18 (p=0.05) whereas the level of TDCCA in urine >50th percentile was related to XY disomy (p=0.04) and disomy of chromosome 21 (p=0.05). Urinary 3PBA level ⩽50 and >50 percentile was related to disomy of sex chromosomes: XY disomy (p=0.05 and p=0.02 respectively), Y disomy (p=0.04 and 0.02 respectively), disomy of chromosome 21 (p=0.04 and p=0.04 respectively) and total disomy (p=0.03 and p=0.04 respectively). Additionally disomy of chromosome 18 was positively associated with urinary level of 3PBA >50 percentile (p=0.03). The results reported here are found that pyrethroids may be a sperm aneugens. These findings may be of concern due to increased pyrethroid use and prevalent human exposure.
authors: Department of Gynecology and Reproduction, "Gameta" Hospital, 34/36 Rudzka St, 95-030 Rzgów, Poland. Electronic address: mradwan@gameta.pl.
authors: Michał
authors: M
authors: Radwan
authors: Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, 8 Teresy St, 91-362 Lodz, Poland. Electronic address: joannaj@imp.lodz.pl.
authors: Joanna
authors: J
authors: Jurewicz
authors: Department of Toxicology, Medical University of Gdańsk, 107 Hallera St, Gdańsk, Poland.
authors: Bartosz
authors: B
authors: Wielgomas
authors: Department of Toxicology, Medical University of Gdańsk, 107 Hallera St, Gdańsk, Poland.
authors: Marta
authors: M
authors: Piskunowicz
authors: Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, 8 Teresy St, 91-362 Lodz, Poland.
authors: Wojciech
authors: W
authors: Sobala
authors: Department of Gynecology and Reproduction, "Gameta" Hospital, 34/36 Rudzka St, 95-030 Rzgów, Poland.
authors: Paweł
authors: P
authors: Radwan
authors: Department of Medical Genetics, Polish Mother's Memorial Hospital, Research Institute, 281/289 Rzgowska St, Lodz, Poland.
authors: Lucjusz
authors: L
authors: Jakubowski
authors: Department of Medical Genetics, Polish Mother's Memorial Hospital, Research Institute, 281/289 Rzgowska St, Lodz, Poland.
authors: Wanda
authors: W
authors: Hawuła
authors: Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, 8 Teresy St, 91-362 Lodz, Poland.
authors: Wojciech
authors: W
authors: Hanke
mesh_terms: D000328:Adult; D000782:Aneuploidy; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008856:Microscopy, Fluorescence; D011044:Poland; D011722:Pyrethrins / Q000652:urine*; D013094:Spermatozoa / Q000187:drug effects*; D055815:Young Adult
chemical_list: D004785:Environmental Pollutants; D011722:Pyrethrins
keywords: Environmental exposure; Pyrethroids; Sperm aneuploidy; Sperm chromosome disomy