title: A phytosterol enriched refined extract of Brassica campestris L. pollen significantly improves benign prostatic hyperplasia (BPH) in a rat model as compared to the classical TCM pollen preparation Qianlie Kang Pule'an Tablets.
abstract: In Qinghai Province, the Brassica campestris L. pollen preparation Qianlie Kang Pule'an Tablet (QKPT) is traditionally used for BPH therapy. However, in QKPT the content of supposedly active phytosterols is relatively low at 2.59%, necessitating high doses for successful therapy. Therefore, a phytosterol enriched (4.54%) refined extract of B. campestris pollen (PE) was developed and compared with QKPT in a BPH rat model. Six groups of rats (n=8 each), namely sham-operated distilled water control, castrated distilled water control, castrated QKPT 2.0g/kg, castrated PE 0.1g/kg, castrated PE 0.2g/kg, and castrated PE 0.4g/kg, were intragastrically treated with the respective daily doses. Testosterone propionate (0.3mg/day) was administered to all castrated rats, while the sham-operated group received placebo injections. After 30 days, the animals were sacrificed and prostates as well as seminal vesicles excised and weighted in order to calculate prostate volume index (PVI) as well as prostate index (PI) and seminal vesicle index (SVI), defined as organ weight in g per 100g body weight. Compared with sham-operated controls, PI (p<0.01), PVI (p<0.01), and SVI (p<0.01) were all significantly increased in all castrated, testosterone treated rats. After treatment with PE at 0.4 and 0.2g/kg or QKPT at 2.0g/kg per day, both indices were significantly reduced (p<0.01) as compared to the castrated distilled water control. For PE at 0.1g/kg per day only PI was significantly reduced (p<0.05). At the highest PE concentration of 0.4g/kg per day both PI and SVI were also significantly reduced when compared to the QKPT group (p<0.05). Both PE and QKPT demonstrated curative effects against BPH in the applied animal model. In its highest dose at 0.4g/kg per day, PE was clearly superior to QKPT.
authors: Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou 310052, PR China; Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Hangzhou 310052, PR China.
authors: Ruwei
authors: R
authors: Wang
authors: Faculty of Medicine, Shimane University, 693-8501 Izumo, Enya 89-1, Japan.
authors: Yuta
authors: Y
authors: Kobayashi
authors: Medical Corporation Soujikai, 541-0046 Osaka, Chuo-ku, Hirano 2-2-2, Japan.
authors: Yu
authors: Y
authors: Lin
authors: Department of Pharmaceutical Biology, Leipzig University, Johannisallee 23, 04103 Leipzig, Germany.
authors: Hans Wilhelm
authors: HW
authors: Rauwald
authors: Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou 310052, PR China; Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Hangzhou 310052, PR China.
authors: Ling
authors: L
authors: Fang
authors: Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou 310052, PR China; Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Hangzhou 310052, PR China.
authors: Hongxiang
authors: H
authors: Qiao
authors: Department of Pharmaceutical Biology, Leipzig University, Johannisallee 23, 04103 Leipzig, Germany; Natural Products Chemistry Research, Department of Food and Nutrition, Sanyo Gakuen University-College, 703-8501 Okayama, Naka-ku, Hirai 1-14-1, Japan. Electronic address: kkuchta@rz.uni-leipzig.de.
authors: Kenny
authors: K
authors: Kuchta
mesh_terms: D000818:Animals; D001937:Brassica / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D008297:Male; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D010840:Phytosterols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011058:Pollen / Q000737:chemistry*; D011467:Prostate / Q000187:drug effects / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043343:Testosterone Propionate
chemical_list: D004365:Drugs, Chinese Herbal; D010840:Phytosterols; D010936:Plant Extracts; D043343:Testosterone Propionate
keywords: Benign prostatic hyperplasia; Brassica campestris L.; Brassicaceae; Phytosterols; Pollen; TCM