title: Molecular epidemiology of group A rotavirus among children admitted to hospital in Salto, Uruguay, 2011-2012: first detection of the emerging genotype G12.
abstract: Group A rotavirus (RVA) is the most important etiologic agent of infant acute gastroenteritis (AGE) worldwide. Detection and molecular characterization of RVA in Salto department, Northwestern region of Uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with AGE, between 0-15 years old, from two hospitals of Salto city during 2011 and 2012. RVA was detected and genotyped by seminested multiplex RT-PCR in order to determine G- and P-genotypes. Positive samples were sequenced and phylogenetic analyses were carried out in order to determine lineages and sub-lineages. RVA were detected in 64 (37%) of the samples and the G and P genotypes observed were: 6% G1P[8], 23% G2P[4]/G2P[X]/GXP[4], 23% G3P[8]/G3P[X], 14% G12P[8]/G12P[X], 16% GXP[8], 1,5% G12P[9], 3% G2P[4]/[8], and 16% non-typeable. VP7 and VP4 genotypes related to DS-1 like gene constellation were prevalent during 2011 and those VP7 and VP4 genotypes related to Wa-like constellation were prevalent during 2012 (mainly represented by G3P[8]). Interestingly, RVA was detected in vomit samples in a high prevalence (41%). RVA was observed mainly in the age group between 1 and 5 years old (75% of the cases), and seasonality with a high detection rate in winter season was observed for the two consecutive years of surveillance. To our knowledge, this study represents the first detection and molecular characterization of RVA in Salto department, Northwestern region of Uruguay; and the first identification of the emerging genotype G12 in the country.
authors: Molecular Virology Laboratory, North Regional, University of Republic, Salto, Uruguay.
authors: Luis Fernando López
authors: LF
authors: Tort
authors: Matías
authors: M
authors: Victoria
authors: Andrés
authors: A
authors: Lizasoain A
authors: Matías
authors: M
authors: Castells
authors: Leticia
authors: L
authors: Maya
authors: Mariela Martínez
authors: MM
authors: Gómez
authors: Edit
authors: E
authors: Arreseigor
authors: Patricia
authors: P
authors: López
authors: Juan
authors: J
authors: Cristina
authors: Jose Paulo Gagliardi
authors: JP
authors: Leite
authors: Rodney
authors: R
authors: Colina
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012307:Risk Factors; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D012621:Seasons; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014505:Urban Population; D014583:Uruguay / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus
keywords: G- and P-genotypes; Salto; Uruguay; group A rotavirus; hospitalized children