title: Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study.
abstract: OBJECTIVE
  In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice.
  
  
  METHODS
  Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides.
  
  
  RESULTS
  Based on analysis of immune response it was observed mice boosted with Ag85B peptides showed significant (p < 0.001) cytokines levels (interferon γ, interleukin 12) and BCG specific antibodies (anti-BCG and anti-purified protein derivative titre) compared to booster dose of BCG, Ag85B and BCG alone.
  
  
  CONCLUSIONS
  Our pilot results suggest that prime boost regimes with Ag85B peptides can boost waning BCG induced immunity and may improve immunogenicity of BCG vaccine. However, lot of work is further needed using experimental model of tuberculosis infection to justify the result.
authors: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, India.
authors: Aliabbas A
authors: 0000-0002-0765-3325
authors: AA
authors: Husain
authors: Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Nagpur, India.
authors: Shubhangi R
authors: 0000-0003-2831-5074
authors: SR
authors: Warke
authors: Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Nagpur, India.
authors: Dewanand R
authors: 0000-0002-4061-909X
authors: DR
authors: Kalorey
authors: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, India.
authors: Hatim F
authors: 0000-0001-6081-6480
authors: HF
authors: Daginawala
authors: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, India.
authors: Girdhar M
authors: 0000-0001-8710-3894
authors: GM
authors: Taori
authors: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, India.
authors: Rajpal S
authors: 0000-0001-6643-0300
authors: RS
authors: Kashyap
keywords: BCG vaccine; Tuberculosis; antigen 85B; antigen 85B peptide
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