title: DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring.
abstract: Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fishes. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.
authors: Departamento de Biologia Geral, Universidade Estadual de Londrina, Centro de Ciências Biológicas, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, 86057-970, Londrina-PR, Brasil.
authors: W
authors: W
authors: Frantine-Silva
authors: Departamento de Biologia Geral, Universidade Estadual de Londrina, Centro de Ciências Biológicas, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, 86057-970, Londrina-PR, Brasil.
authors: S H
authors: SH
authors: Sofia
authors: Departamento de Biologia Animal e Vegetal, Universidade Estadual de Londrina, Centro de Ciências Biológicas, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, 86057-970, Londrina-PR, Brasil.
authors: M L
authors: ML
authors: Orsi
authors: Departamento de Biologia Geral, Universidade Estadual de Londrina, Centro de Ciências Biológicas, Campus Universitário, Rodovia Celso Garcia Cid, PR 445 km 380, 86057-970, Londrina-PR, Brasil.
authors: F S
authors: FS
authors: Almeida
mesh_terms: D000818:Animals; D044822:Biodiversity; D001938:Brazil; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D005618:Fresh Water; D007814:Larva / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D045483:Rivers; D017422:Sequence Analysis, DNA; D015053:Zygote / Q000145:classification
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
keywords: conservation; eggs; fish recruitment; upper Paraná River