title: Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection.
abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4 (DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry; however, changes at positions 288 and 330 can confer permissivity. Position 330 changes the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor impacting DPP4 receptor function. These results provide insight into DPP4 species-specific differences impacting MERS-CoV host range and may inform MERS-CoV mouse model development.
authors: Department of Biology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors: Kayla M
authors: http://orcid.org/0000-0001-7745-6995
authors: KM
authors: Peck
authors: Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors: Adam S
authors: AS
authors: Cockrell
authors: Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors: Boyd L
authors: BL
authors: Yount
authors: Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors: Trevor
authors: T
authors: Scobey
authors: Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA rbaric@unc.edu mark_heisem@med.unc.edu.
authors: Ralph S
authors: RS
authors: Baric
authors: Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA rbaric@unc.edu mark_heisem@med.unc.edu.
authors: Mark T
authors: MT
authors: Heise
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018352:Coronavirus Infections / Q000517:prevention & control*; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006031:Glycosylation; D051379:Mice; D065207:Middle East Respiratory Syndrome Coronavirus / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013045:Species Specificity; D053586:Virus Internalization
chemical_list: D018819:Dipeptidyl Peptidase 4; C545245:Dpp4 protein, mouse
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