title: Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis.
abstract: Chlorogenic acid (CGA) exhibits various biological properties, including the inhibition of oxidation, obesity, apoptosis and tumorigenesis. CGA is also able to promote cell survival and proliferation. The aim of the present study was to determine the effects and underlying molecular mechanisms of CGA on the adipogenesis of bone marrow‑derived mesenchymal stem cells (BMSCs). Treatment with CGA had a marginal effect on cell proliferation, by promoting the expression levels of phosphorylated Akt and cyclin D1. Furthermore, treatment with CGA also upregulated the phosphorylation of extracellular signal‑regulated kinase (Erk) and inhibited the adipocyte differentiation of BMSCs by inhibiting the expression of peroxisome proliferator‑activated receptor (PPAR)γ and CCAAT/enhancer binding protein (C/EBP)α. However, knockdown of the expression of Shp2 attenuated CGA‑induced proliferation and inhibited the phosphorylation of Akt and expression of cyclin D1. Furthermore, CGA treatment upregulated Erk phosphorylation and decreased the expression levels of PPARγ and CEBPα, which was inhibited by treatment with the Shp2 PTPase activity inhibitor, NSC‑87877. The results of the present study suggested that CGA‑induced Akt and Erk pathways regulate proliferation and differentiation and that Shp2 is important in the proliferation and differentiation of BMSCs.
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Rong-Ping
authors: RP
authors: Zhou
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Ming-Tao
authors: MT
authors: Deng
authors: Pharmacy College, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors: Lan-Ying
authors: LY
authors: Chen
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Ning
authors: N
authors: Fang
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Chuan
authors: C
authors: Du
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Lin-Pan
authors: LP
authors: Chen
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Ye-Qing
authors: YQ
authors: Zou
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Jiang-Hua
authors: JH
authors: Dai
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Mei-Lan
authors: ML
authors: Zhu
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Wei
authors: W
authors: Wang
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Si-Jian
authors: SJ
authors: Lin
authors: Pharmacy College, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors: Rong-Hua
authors: RH
authors: Liu
authors: Orthopaedic Department, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors: Jun
authors: J
authors: Luo
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D019938:Cyclin D1 / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C512715:NSC-87877; D047495:PPAR gamma; D011804:Quinolines; D034741:RNA, Small Interfering; D019938:Cyclin D1; D002726:Chlorogenic Acid; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11