title: Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.
abstract: Puerarin, a traditional Chinese medicine, exerts a powerful neuroprotective effect in cerebral ischemia/reperfusion injury, but its mechanism is unknown. Here, we established rat models of middle cerebral artery ischemia/reperfusion injury using the suture method. Puerarin (100 mg/kg) was administered intraperitoneally 30 minutes before middle cerebral artery occlusion and 8 hours after reperfusion. Twenty-four hours after reperfusion, we found that puerarin significantly improved neurological deficit, reduced infarct size and brain water content, and notably diminished the expression of Toll-like receptor-4, myeloid differentiation factor 88, nuclear factor kappa B and tumor necrosis factor-α in the ischemic region. These data indicate that puerarin exerts an anti-inflammatory protective effect on brain tissue with ischemia/reperfusion damage by downregulating the expression of multiple inflammatory factors.
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Feng
authors: F
authors: Zhou
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China ; Medical College, Ningbo University, Ningbo, Zhejiang Province, China.
authors: Liang
authors: L
authors: Wang
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Panpan
authors: P
authors: Liu
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Weiwei
authors: W
authors: Hu
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Xiangdong
authors: X
authors: Zhu
authors: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Hong
authors: H
authors: Shen
authors: Department of Anesthesiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Yuanyuan
authors: Y
authors: Yao
keywords: Toll-like receptor-4; brain injury; cerebral ischemia; inflammatory reaction; middle cerebral artery occlusion; myeloid differentiation factor 88; nerve regeneration; neural regeneration; nuclear factor kappa B; puerarin; rats; reperfusion injury; tumor necrosis factor-α
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