title: A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis.
abstract: Heterotrimeric G-proteins are versatile regulators involved in diverse cellular processes in eukaryotes. In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate drought tolerance in Arabidopsis. Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling. Our study found that transcript levels of three ABA responsive genes, AtMPK6, AtVIP1 and AtMYB44 (downstream gene of AtVIP1), were significantly up-regulated in agb1-2 lines after ABA or drought treatments. Other ABA-responsive and drought-inducible genes, such as RD29A (downstream gene of AtMYB44), were also up-regulated in agb1-2 lines. Furthermore, overexpression of AtVIP1 resulted in hypersensitivity to ABA at seed germination and seedling stages, and significantly enhanced drought tolerance in transgenic plants. These results suggest that AGB1 was involved in the ABA signaling pathway and drought tolerance in Arabidopsis through down-regulating the AtMPK6, AtVIP1 and AtMYB44 cascade.
authors: College of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: Dong-bei
authors: DB
authors: Xu
authors: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: Ming
authors: M
authors: Chen
authors: College of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: Ya-nan
authors: YN
authors: Ma
authors: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: Zhao-shi
authors: ZS
authors: Xu
authors: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: Lian-cheng
authors: LC
authors: Li
authors: College of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Yao-feng
authors: YF
authors: Chen
authors: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
authors: You-zhi
authors: YZ
authors: Ma
mesh_terms: D000040:Abscisic Acid / Q000502:physiology; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D003681:Dehydration / Q000378:metabolism; D015536:Down-Regulation; D055864:Droughts; D044387:GTP-Binding Protein beta Subunits / Q000502:physiology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000502:physiology; D015398:Signal Transduction
chemical_list: C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044387:GTP-Binding Protein beta Subunits; D010937:Plant Growth Regulators; C432104:VIP1 protein, Arabidopsis; D000040:Abscisic Acid; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases
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