title: Interaction and colocalization of HERMES/RBPMS with NonO, PSF, and G3BP1 in neuronal cytoplasmic RNP granules in mouse retinal line cells.
abstract: HERMES, also called RBPMS, is a conserved RNA binding protein with a single RNA recognition motif (RRM) that is abundantly expressed in retinal ganglion cells (RGCs) and in the heart in vertebrates. Here, we identified NonO and PSF as the interacting proteins of HERMES only when the neuronal differentiation of the retinal cell line RGC-5 was induced. Although NonO and PSF are nuclear paraspeckle components, these proteins formed cytoplasmic granules with HERMES in the neurites. G3BP1, a component of stress granules, was also colocalized to the granules, interacting with NonO and HERMES even in the absence of cellular stress. Consistent with a previous report that KIF5 interacts with neuronal granules, the localization of KIF5A overlapped with the cytoplasmic granules in differentiated RGC-5 cells. Thus, our study strongly suggests that the cytoplasmic granule containing HERMES, NonO, PSF, and G3BP1 is a neuronal RNA-protein granule that is transported in neurites during retinal differentiation.
authors: Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan.
authors: Mari T
authors: MT
authors: Furukawa
authors: Hiroshi
authors: H
authors: Sakamoto
authors: Kunio
authors: K
authors: Inoue
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000072670:PTB-Associated Splicing Factor; D000075223:Poly-ADP-Ribose Binding Proteins; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C507512:Kif5A protein, mouse; C500331:Nono protein, mouse; D000072670:PTB-Associated Splicing Factor; D000075223:Poly-ADP-Ribose Binding Proteins; D047428:Protein Kinase Inhibitors; D000067776:RNA Recognition Motif Proteins; D016601:RNA-Binding Proteins; C119265:Rbpms protein, mouse; D017411:Ribonucleoproteins, Small Nuclear; D004265:DNA Helicases; C492055:G3bp1 protein, mouse; D020365:RNA Helicases; D016547:Kinesins; D019311:Staurosporine