title: Developmental genetic mechanisms of C4 syndrome based on transcriptome analysis of C3 cotyledons and C4 assimilating shoots in Haloxylon ammodendron.
abstract: It is believed that transferring the C4 engine into C3 crops will greatly increase the yields of major C3 crops. Many efforts have been made since the 1960s, but relatively little success has been achieved because C4plant traits, referred to collectively as C4 syndrome, are very complex, and little is known about the genetic mechanisms involved. Unfortunately, there exists no ideal genetic model system to study C4 syndrome. It was previously reported that the Haloxylon species have different photosynthetic pathways in different photosynthetic organs, cotyledons and assimilating shoots. Here, we took advantage of the developmental switch from the C3 to the C4 pathway to study the genetic mechanisms behind this natural transition. We compared the transcriptomes of cotyledons and assimilating shoots using mRNA-Seq to gain insight into the molecular and cellular events associated with C4 syndrome. A total of 2959 differentially expressed genes [FDR ≤ 0.001 and abs (|log2(Fold change)| ≥ 1)] were identified, revealing that the transcriptomes of cotyledons and assimilating shoots are considerably different. We further identified a set of putative regulators of C4 syndrome. This study expands our understanding of the development of C4 syndrome and provides a new model system for future studies on the C3-to- C4 switch mechanism.
authors: Key Laboratory of Systems Biology, Shandong Normal University, Jinan, Shandong, China; Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors: Yuanyuan
authors: Y
authors: Li
authors: Key Laboratory of Plant Stress Research, Shandong Normal University, Jinan, Shandong, China.
authors: Xiuling
authors: X
authors: Ma
authors: Key Laboratory of Plant Stress Research, Shandong Normal University, Jinan, Shandong, China.
authors: Jialei
authors: J
authors: Zhao
authors: Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors: Jiajia
authors: J
authors: Xu
authors: Key Laboratory of Plant Stress Research, Shandong Normal University, Jinan, Shandong, China.
authors: Junfeng
authors: J
authors: Shi
authors: Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors: Xin-Guang
authors: XG
authors: Zhu
authors: Key Laboratory of Plant Stress Research, Shandong Normal University, Jinan, Shandong, China.
authors: Yanxiu
authors: Y
authors: Zhao
authors: Key Laboratory of Plant Stress Research, Shandong Normal University, Jinan, Shandong, China.
authors: Hui
authors: H
authors: Zhang
mesh_terms: D027703:Amaranthaceae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D057486:Carbon Cycle / Q000235:genetics*; D019069:Cell Respiration / Q000235:genetics; D018548:Cotyledon / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics; D010788:Photosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D015854:Up-Regulation
chemical_list: D014157:Transcription Factors
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