title: Brain clearing for connectomics.
abstract: The article reflects on the development of tissue clearing methods in the last decade. This technological innovation has been achieved in the neuroscience community thanks to the combined efforts of molecular biologists, brain anatomists and light microscopists. Although this technology is becoming increasingly diversified, it mostly aims at the large-scale, three-dimensional reconstruction of brain structures.
authors: Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako city, Saitama, 351-0198, Japan Biotechnological Optics Research Team, Center for Advanced Photonics, RIKEN, 2-1 Hirosawa, Wako city, Saitama, 351-0198, Japan matsushi@brain.riken.go.jp.
authors: Atsushi
authors: A
authors: Miyawaki
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D063132:Connectome / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008322:Mammals; D008856:Microscopy, Fluorescence / Q000379:methods; D008967:Molecular Biology; D009488:Neurosciences
keywords: fluorescence microscopy; large-scale 3D reconstruction; optical clearing