title: Validation of serum biomarkers derived from proteomic analysis for the early screening of preeclampsia.
abstract: OBJECTIVE
  To examine the potential value of previously identified biomarkers using proteomics in early screening for preeclampsia (PE).
  
  
  METHODS
  24 blood samples from women who subsequently developed PE and 48 from uncomplicated pregnancies were obtained at 11-13 weeks and analysed after delivery. Cystatin-C, sVCAM-1, and Pappalysin-1 were quantified by ELISA. Maternal characteristics and medical history were recorded.
  
  
  RESULTS
  Median values of Cystatin-C, sVCAM-1, and Pappalysin-1 in the PE group as compared to controls were 909.1 gEq/mL versus 480.0 gEq/mL, P = .000, 832.0 gEq/mL versus 738.8 gEq/mL, P = .024, and 234.4 gEq/mL versus 74.9 gEq/mL, P = .064, respectively. Areas under the receiver-operating characteristic curves (AUC, standard error (SE)) for predicting PE were Cystatin-C: 0.90 (SE 0.04), VCAM-1: 0.66 (SE 0.074), and Pappalysin-1: 0.63 (SE 0.083). To discriminate between cases at risk for PE and normal controls, cut-off values of 546.8 gEq/mL for Cystatin-C, 1059.5 gEq/mL for sVCAM-1, and 220.8 gEq/mL for Pappalysin-1 were chosen, providing sensitivity of 91%, 41%, and 54% and specificity of 85%, 100%, and 95%, respectively.
  
  
  CONCLUSIONS
  sVCAM-1 and Pappalysin-1 do not improve early screening for PE. Cystatin-C, however, seems to be associated with subsequent PE development, but larger studies are necessary to validate these findings.
authors: Department of Medical Genetics, Athens University, "Aghia Sofia" Children's Hospital, 11527 Athens, Greece.
authors: Aggeliki
authors: A
authors: Kolialexi
authors: 2nd Department of Pediatrics, Athens University, 11527 Athens, Greece.
authors: Dimitrios
authors: D
authors: Gourgiotis
authors: 1st Department of Obstetrics & Gynecology, Athens University, 11528 Athens, Greece.
authors: George
authors: G
authors: Daskalakis
authors: 2nd Department of Pediatrics, Athens University, 11527 Athens, Greece.
authors: Antonis
authors: A
authors: Marmarinos
authors: Department of Medical Genetics, Athens University, "Aghia Sofia" Children's Hospital, 11527 Athens, Greece.
authors: Alexandra
authors: A
authors: Lykoudi
authors: Department of Medical Genetics, Athens University, "Aghia Sofia" Children's Hospital, 11527 Athens, Greece.
authors: Danai
authors: D
authors: Mavreli
authors: Department of Medical Genetics, Athens University, "Aghia Sofia" Children's Hospital, 11527 Athens, Greece.
authors: Ariadni
authors: A
authors: Mavrou
authors: 1st Department of Obstetrics & Gynecology, Athens University, 11528 Athens, Greece.
authors: Nikolas
authors: N
authors: Papantoniou
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D055316:Cystatin C / Q000097:blood*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011266:Pregnancy-Associated Plasma Protein-A / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*
chemical_list: D015415:Biomarkers; D055316:Cystatin C; D020543:Proteome; D019010:Vascular Cell Adhesion Molecule-1; D011266:Pregnancy-Associated Plasma Protein-A; C579283:PAPPA protein, human
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