title: Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.
abstract: In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay.
authors: Max-Planck-Institut für biophysikalische Chemie (MPI-bpc), Am Fassberg 11, 37077, Goettingen, Germany, shevelkov@fmp-berlin.de.
authors: Veniamin
authors: V
authors: Chevelkov
authors: ShengQi
authors: S
authors: Xiang
authors: Karin
authors: K
authors: Giller
authors: Stefan
authors: S
authors: Becker
authors: Adam
authors: A
authors: Lange
authors: Bernd
authors: B
authors: Reif
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D060526:Magnetic Fields; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D011506:Proteins; D012997:Solvents; D014867:Water; D003903:Deuterium
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