title: Searching for biomarkers of comorbidities in sera of treated HIV-infected patients by isoelectric focusing.
abstract: Based on their characteristics, we hypothesized that the following parameters, namely collagen IV, glutathione S-transferase, secretory component (SC), and AMP-activated protein kinase α1α2 may be useful serum markers in the detection of comorbidities in treated HIV-infected patients. These parameters were determined in 204 HIV-infected patients and 35 controls by using IEF and densitometry. Collagen IV was undetectable in controls and the majority of HIV-infected patients. Twenty-two HIV-infected patients presented significantly elevated levels of collagen IV, most of them were coinfected with hepatitis C virus and/or hepatitis B virus. SC was undetectable in controls. SC was significantly increased in 81 HIV-infected patients and significantly correlated with aspartate aminotransferase (r = 0.267, p = 0.0049), alkaline phosphatase (r = 0.309, p = 0.0011), and γ-glutamyl-transferase (r = 0.264, p = 0.0054). Glutathione S-transferase levels of HIV-infected patients were significantly higher than the controls (3779 ± 5860 vs. 785 ± 71 DU, p = 0.0007) and significantly correlated with serum urea (r = 0.204, p = 0.0038), triglycerides (r = 0.209, p = 0.0033), and lipase (r = 0.219, p = 0.0025). AMP-activated protein kinase α1α2 levels of HIV-infected patients were significantly higher than the controls (5676 ± 6248 vs. 1189 ± 6248 DU, p = 0.0009). Further studies are needed to demonstrate the relevance of these results to diagnose non-AIDS-related illnesses in HIV-infected patients.
authors: Fédération de Biochimie de l'hôpital Edouard Herriot, Lyon, France.
authors: Etienne
authors: E
authors: Malvoisin
authors: Service des Maladies Infectieuses et Tropicales de l'hôpital Edouard Herriot, Lyon, France.
authors: Djamila
authors: D
authors: Makhloufi
authors: Service des Maladies Infectieuses et Tropicales de l'hôpital Edouard Herriot, Lyon, France.
authors: Jean-Michel
authors: JM
authors: Livrozet
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D024141:Collagen Type IV / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D015658:HIV Infections / Q000097:blood* / Q000150:complications*; D006509:Hepatitis B / Q000097:blood / Q000150:complications*; D006526:Hepatitis C / Q000097:blood / Q000150:complications*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*
chemical_list: D015415:Biomarkers; D024141:Collagen Type IV; D005982:Glutathione Transferase; D055372:AMP-Activated Protein Kinases
keywords: AMP-activated protein kinase; Glutathione S-transferase; HIV; Isoelectric focusing; Secretory component; Type IV collagen