title: Identification of a sustained neurogenic zone at the dorsal surface of the adult mouse hippocampus and its regulation by the chemokine SDF-1.
abstract: We identified a previously unknown neurogenic region at the dorsal surface of the hippocampus; (the "subhippocampal zone," SHZ) in the adult brain. Using a reporter mouse in which SHZ cells and their progeny could be traced through the expression of EGFP under the control of the CXCR4 chemokine receptor promoter we observed the presence of a pool of EGFP expressing cells migrating in direction of the dentate gyrus (DG), which is maintained throughout adulthood. This population appeared to originate from the SHZ where cells entered a caudal migratory stream (aCMS) that included the fimbria, the meninges and the DG. Deletion of CXCR4 from neural stem cells (NSCs) or neuroinflammation resulted in the appearance of neurons in the DG, which were the result of migration of NSCs from the SHZ. Some of these neurons were ectopically placed. Our observations indicate that the SHZ is a neurogenic zone in the adult brain through migration of NSCs in the aCMS. Regulation of CXCR4 signaling in these cells may be involved in repair of the DG and may also give rise to ectopic granule cells in the DG in the context of neuropathology.
authors: Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Abdelhak
authors: A
authors: Belmadani
authors: Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Dongjun
authors: D
authors: Ren
authors: Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Bula J
authors: BJ
authors: Bhattacharyya
authors: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Katharina B
authors: KB
authors: Rothwangl
authors: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Thomas J
authors: TJ
authors: Hope
authors: Department of Medicine and Rheumatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Harris
authors: H
authors: Perlman
authors: Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
authors: Richard J
authors: RJ
authors: Miller
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D018345:Mice, Knockout; D058953:Neural Stem Cells; D055495:Neurogenesis / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*
chemical_list: C514936:CXCR4 protein, mouse; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D005456:Fluorescent Dyes; D019718:Receptors, CXCR4; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: CXCR4; SDF-1; adult neurogenesis; chemokines; dentate gyrus
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