title: RIG-I specifically mediates group II type I IFN activation in nervous necrosis virus infected zebrafish cells.
abstract: The type I interferon (IFN) response has been shown to be crucial for the survival of zebrafish larvae infected with nervous necrosis virus (NNV). Teleost type I IFNs can be divided into two groups, based on their cysteine content. While teleost group I IFNs have been extensively studied in terms of their regulation and anti-viral properties, the characteristics of teleost group II IFNs have been relatively unexplored. In this study, we describe the mechanism by which group II IFNs are activated in response to NNV infection in a zebrafish cell line, by focusing on the relationship between type I IFNs and pattern recognition receptors. Expression profile analysis of infected cells by microarray and qPCR revealed signaling activation of two pattern recognition receptors (PRRs): RIG-I like receptors (RLRs) and MyD88-dependent Toll-like receptors (TLRs). Knockdown of retinoic acid-inducible gene I (RIG-I) specifically repressed induction of group II IFNs (IFNϕ2, IFNϕ3) by NNV infection. Furthermore, Ingenuity Pathway Analysis (IPA) was used to demonstrate that RIG-I knockdown results in down-regulation of the inflammatory response in NNV-infected cells. Taken together, our results indicate that RIG-I plays an essential role in zebrafish group II type I IFN induction and the inflammatory response to NNV infection.
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan.
authors: Hsu-Yu
authors: HY
authors: Chen
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Wangta
authors: W
authors: Liu
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan.
authors: Sung-Yu
authors: SY
authors: Wu
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan.
authors: Pinwen Peter
authors: PP
authors: Chiou
authors: Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
authors: Yen-Hsing
authors: YH
authors: Li
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Yen-Chun
authors: YC
authors: Chen
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan.
authors: Gen-Hwa
authors: GH
authors: Lin
authors: Department of Aquaculture, The College of Life and Resource Science, National Taiwan Ocean University, Keelung 202, Taiwan. Electronic address: mingwei@mail.ntou.edu.tw.
authors: Ming-Wei
authors: MW
authors: Lu
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 115, Taiwan. Electronic address: jlwu@gate.sinica.edu.tw.
authors: Jen-Leih
authors: JL
authors: Wu
mesh_terms: D000818:Animals; D015536:Down-Regulation; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D007370:Interferon Type I / Q000235:genetics* / Q000378:metabolism; D030381:Nodaviridae / Q000502:physiology*; D012327:RNA Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D051192:Receptors, Pattern Recognition / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D007370:Interferon Type I; D051192:Receptors, Pattern Recognition; D051193:Toll-Like Receptors; D029961:Zebrafish Proteins; C000599980:ddx58 protein, zebrafish
keywords: Inflammatory response; NNV; RIG-I; Type I IFN; Zebrafish