title: Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement.
abstract: OBJECTIVE
  To evaluate the antibacterial and antifungal properties of calcium-based cement, Biodentine (Ca3SiO2), compared to commercial glass ionomer cements (GICs) and mineral trioxide aggregate (MTA).
  
  
  METHODS
  Pellets of GICs, ProRoot MTA, and Biodentine were prepared to test the influence of these cements on the growth of four oral microbial strains: Streptococcus mutans, Enterococcus faecalis, Escherichia coli, and Candida albicans; using agar diffusion method. Wells were formed by removing the agar and the manipulated materials were immediately placed in the wells. The pellets were lodged in seeded plates and the growth inhibition diameter around the material was measured after 24-72 h incubation at 37°C. The data were analyzed using analysis of variance (ANOVA) test to compare the differences among the three cements at different concentrations.
  
  
  RESULTS
  Test indicates that the antimicrobial activity of Biodentine, on all the microorganisms tested, was very strong, showing a mean inhibition zone of 3.2 mm, which extends over time towards all the strains. For Biodentine, GIC, and MTA, the diameters of the inhibition zones for S. mutans were significantly larger than for E. faecalis, Candida, and E. coli (P < 0.05).
  
  
  CONCLUSIONS
  All materials showed antimicrobial activity against the tested strains except for GIC on Candida. Largest inhibition zone was observed for Streptococcus group. Biodentine created larger inhibition zones than MTA and GIC.
authors: Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India.
authors: Vankayala
authors: V
authors: Bhavana
authors: Department of Conservative Dentistry and Endodontics, Saraswati Dhanawantari Dental College, Parbhani, Maharashtra, India.
authors: Krishna Popuri
authors: KP
authors: Chaitanya
authors: Department of Conservative Dentistry and Endodontics, Sri Venkata Sai Institute of Dental Sciences, Mahabubnagar, Telangana, India.
authors: Padma
authors: P
authors: Gandi
authors: Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India.
authors: Jayaprakash
authors: J
authors: Patil
authors: Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India.
authors: Binoy
authors: B
authors: Dola
authors: Department of Prosthodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India.
authors: Rahul B
authors: RB
authors: Reddy
keywords: Antibacterial activity; MTA; giodentine; inhibition zone
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