title: Enzyme degradable polymersomes from hyaluronic acid-block-poly(ε-caprolactone) copolymers for the detection of enzymes of pathogenic bacteria.
abstract: We introduce a new hyaluronidase-responsive amphiphilic block copolymer system, based on hyaluronic acid (HYA) and polycaprolactone (PCL), that can be assembled into polymersomes by an inversed solvent shift method. By exploiting the triggered release of encapsulated dye molecules, these HYA-block-PCL polymersomes lend themselves as an autonomous sensing system for the detection of the presence of hyaluronidase, which is produced among others by the pathogenic bacterium Staphylococcus aureus. The synthesis of the enzyme-responsive HYA-block-PCL block copolymers was carried out by copper-catalyzed Huisgen 1,3-dipolar cycloaddition of ω-azide-terminated PCL and ω-alkyne-functionalized HYA. The structure of the HYA-block-PCL assemblies and their enzyme-triggered degradation and concomitant cargo release were investigated by dynamic light scattering, fluorescence spectroscopy, confocal laser-scanning microscopy, scanning and transmission electron, and atomic force microscopy. As shown, a wide range of reporter dye molecules as well as antimicrobials can be encapsulated into the vesicles during formation and are released upon the addition of hyaluronidase.
authors: Physical Chemistry I, Department of Chemistry and Biology, University of Siegen , Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.
authors: Simon
authors: S
authors: Haas
authors: Nicole
authors: N
authors: Hain
authors: Mohammad
authors: M
authors: Raoufi
authors: Stephan
authors: S
authors: Handschuh-Wang
authors: Tao
authors: T
authors: Wang
authors: Xin
authors: X
authors: Jiang
authors: Holger
authors: H
authors: Schönherr
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000737:chemistry; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000737:chemistry*; D007700:Kinetics; D053769:Nanocapsules / Q000737:chemistry; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D053769:Nanocapsules; D011091:Polyesters; C016240:polycaprolactone; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase