title: One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates.
abstract: Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1-day pulsed-field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.
authors: Department of Microbiology, University of Bialystok, Bialystok, Poland.
authors: Paulina S
authors: PS
authors: Kaminska
authors: Krzysztof
authors: K
authors: Fiedoruk
authors: Dominika
authors: D
authors: Jankowska
authors: Jacques
authors: J
authors: Mahillon
authors: Karol
authors: K
authors: Nowosad
authors: Ewa
authors: E
authors: Drewicka
authors: Monika
authors: M
authors: Zambrzycka
authors: Izabela
authors: I
authors: Swiecicka
mesh_terms: D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity*; D047630:Depsipeptides / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D014839:Vomiting / Q000382:microbiology
chemical_list: D047630:Depsipeptides; C089294:cereulide
keywords: Bacillus cereus; Emetic isolates; PFGE; Rapid protocol