title: Steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in G. barbadense and G. hirsutum.
abstract: In this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in Gossypium barbadense and Gossypium hirsutum species. Sequential 1% H2SO4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. By comparison, extremely high concentration alkali (16% NaOH) pretreatment with raw stalks resulted in the highest hexoses yields, but it had the lowest sugar-ethanol conversion rates. Characterization of wall polymer features indicated that biomass saccharification was enhanced with steam explosion by largely reducing cellulose DP and extracting hemicelluloses. It also showed that cellulose crystallinity and arabinose substitution degree of xylans were the major factors on biomass digestibility in cotton stalks. Hence, this study has provided the insights into cell wall modification and biomass process technology in cotton stalks and beyond.
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Yu
authors: Y
authors: Huang
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Xiaoyang
authors: X
authors: Wei
authors: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China.
authors: Shiguang
authors: S
authors: Zhou
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Mingyong
authors: M
authors: Liu
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Yuanyuan
authors: Y
authors: Tu
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Ao
authors: A
authors: Li
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Peng
authors: P
authors: Chen
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Yanting
authors: Y
authors: Wang
authors: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
authors: Xuewen
authors: X
authors: Zhang
authors: Institute of Agricultural Sciences and Technology, Agricultural Production Division, Xinjiang Production and Construction Corps, Alar Xinjiang 843300, China.
authors: Hongzhong
authors: H
authors: Tai
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Liangcai
authors: L
authors: Peng
authors: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xiatao@mail.hzau.edu.cn.
authors: Tao
authors: T
authors: Xia
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D003368:Gossypium / Q000737:chemistry*; D006601:Hexoses / Q000032:analysis; D008031:Lignin / Q000032:analysis; D058105:Polymerization; D012972:Sodium Hydroxide / Q000494:pharmacology; D013227:Steam; D013464:Sulfuric Acids / Q000494:pharmacology; D014866:Waste Products
chemical_list: D006601:Hexoses; D013227:Steam; D013464:Sulfuric Acids; D014866:Waste Products; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; C033158:sulfuric acid
keywords: Biomass saccharification; Cellulose DP; Cotton stalks; Steam explosion; Yeast fermentation