title: Microfluidic Single-Cell Analysis with Affinity Beads.
abstract: A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green).
authors: Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors: Michael
authors: M
authors: Werner
authors: Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors: Raghavendra
authors: R
authors: Palankar
authors: Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors: Loïc
authors: L
authors: Arm
authors: Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors: Ruud
authors: R
authors: Hovius
authors: Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors: Horst
authors: H
authors: Vogel
mesh_terms: D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D052898:Optical Tweezers
keywords: affinity beads; microfluidics; optical tweezers; photochemical internalization; single-cell analysis