title: Exposures of zebrafish through diet to three environmentally relevant mixtures of PAHs produce behavioral disruptions in unexposed F1 and F2 descendant.
abstract: The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades. PAHs are hydrophobic molecules which can accumulate in high concentrations in sediments acting then as major secondary sources. Fish contamination can occur through contact or residence nearby sediments or though dietary exposure. In this study, we analyzed certain physiological traits in unexposed fish (F1) issued from parents (F0) exposed through diet to three PAH mixtures at similar and environmentally relevant concentrations but differing in their compositions. For each mixture, no morphological differences were observed between concentrations. An increase in locomotor activity was observed in larvae issued from fish exposed to the highest concentration of a pyrolytic (PY) mixture. On the contrary, a decrease in locomotor activity was observed in larvae issued from heavy oil mixture (HO). In the case of the third mixture, light oil (LO), a reduction of the diurnal activity was observed during the setup of larval activity. Behavioral disruptions persisted in F1-PY juveniles and in their offspring (F2). Endocrine disruption was analyzed using cyp19a1b:GFP transgenic line and revealed disruptions in PY and LO offspring. Since no PAH metabolites were dosed in larvae, these findings suggest possible underlying mechanisms such as altered parental signaling molecule and/or hormone transferred in the gametes, eventually leading to early imprinting. Taken together, these results indicate that physiological disruptions are observed in offspring of fish exposed to PAH mixtures through diet.
authors: Ecotoxicology Laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Caroline
authors: C
authors: Vignet
authors: Fisheries laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Lucette
authors: L
authors: Joassard
authors: Fisheries laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Laura
authors: L
authors: Lyphout
authors: Fisheries laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Tiphaine
authors: T
authors: Guionnet
authors: Ecotoxicology Laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Manon
authors: M
authors: Goubeau
authors: University of Bordeaux 1, EPOC, UMR CNRS 5805, 33405, Talence, France.
authors: Karyn
authors: K
authors: Le Menach
authors: Unité d'Ecotoxicologie in vitro et in vivo, Direction des Risques Chroniques, INERIS, 60550, Verneuil-en-Halatte, France.
authors: François
authors: F
authors: Brion
authors: INSERM U1085, Research Institute in Health, Environment and Occupation, Team NEED, Case 1302Université de Rennes 1 Campus de Beaulieu, 35 042, Rennes cedex, France.
authors: Olivier
authors: O
authors: Kah
authors: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
authors: Bon-Chu
authors: BC
authors: Chung
authors: University of Bordeaux 1, EPOC, UMR CNRS 5805, 33405, Talence, France.
authors: Hélène
authors: H
authors: Budzinski
authors: Fisheries laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors: Marie-Laure
authors: ML
authors: Bégout
authors: Ecotoxicology Laboratory, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France. xcousin@ifremer.fr.|INRA LPGP, Campus de Beaulieu, 35042, Rennes, France. xcousin@ifremer.fr.
authors: Xavier
authors: X
authors: Cousin
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced; D001141:Aromatase / Q000378:metabolism; D004032:Diet; D052244:Endocrine Disruptors / Q000633:toxicity*; D018392:Genomic Imprinting / Q000187:drug effects; D007814:Larva / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D059392:Petroleum Pollution; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D013550:Swimming; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D001141:Aromatase
keywords: Anxiety-like behavior; Danio rerio; Heavy oil; Light crude oil; Locomotor activity; Offspring; Photomotor response; Polycyclic aromatic hydrocarbon; Transgenerational effect
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