title: Overexpression of Tbx3 is correlated with Epithelial-Mesenchymal Transition phenotype and predicts poor prognosis of colorectal cancer.
abstract: OBJECTIVE
  To investigate the clinical significance of Tbx3 in colorectal cancer (CRC) and the possible association between Tbx3 expression and Epithelial- Transition Mesenchymal (EMT) phenotype.
  
  
  METHODS
  Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and western blotting were employed to evaluate the expression of Tbx3 in 30 fresh CRC and matched normal tissues. Using immunochemistry, protein level of Tbx3 and EMT markers (E-cadherin and N-cadherin) were identified in 150 pairs of paraffin-embedded specimen.
  
  
  RESULTS
  The results of qRT-PCR and western blotting showed that Tbx3 expression was higher in CRC tissues than in corresponding normal tissues. The statistical analysis based on immunohistochemical evaluation suggested that Tbx3 aberrant expression was significantly associated with tumor size (P=0.049), differentiation (P=0.032), invasion (P=0.019), lymph node metastasis (P=0.049) and TNM stage (P=0.018). Patients who displayed high expression of Tbx3 may achieve a poorer overall survival (OS) and disease-free survival (DFS), compared to those with low expression of Tbx3. This tendency was also observed in patients with intermediate levels of disease (II and III stage). The multivariate analysis indicated Tbx3 expression could independently predict the outcome of CRC patients. Interestingly, correlation analysis suggested Tbx3 expression was negatively correlated with E-cadherin expression, but positively correlated with N-cadherin expression.
  
  
  CONCLUSIONS
  Tbx3 may promote CRC progression by involving EMT program and has the potential to be an effective prognostic predictor for CRC patients.
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Ze-Zhi
authors: ZZ
authors: Shan
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Xue-Bing
authors: XB
authors: Yan
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Lei-Lei
authors: LL
authors: Yan
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Yuan
authors: Y
authors: Tian
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Qing-Cai
authors: QC
authors: Meng
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Wang-Wang
authors: WW
authors: Qiu
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Zhen
authors: Z
authors: Zhang
authors: Department of General Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200233, P. R. China.
authors: Zhi-Ming
authors: ZM
authors: Jin
keywords: Epithelial-Mesenchymal Transition; Tbx3; biomarker; colorectal cancer; prognosis
references: J Biol Chem. 2011 Mar 11;286(10):8425-36
references: 21189255
references: J Cancer. 2014 Mar 16;5(4):272-80
references: 24790655
references: Anat Cell Biol. 2014 Mar;47(1):1-11
references: 24693477
references: Int J Biochem Cell Biol. 2012 Dec;44(12):2144-51
references: 22981632
references: Histopathology. 2013 Nov;63(5):603-15
references: 23991632
references: Int J Biochem Cell Biol. 2007;39(12):2153-60
references: 17825600
references: Bioessays. 1998 Jan;20(1):9-19
references: 9504043
references: CA Cancer J Clin. 2014 Mar-Apr;64(2):104-17
references: 24639052
references: Dev Growth Differ. 2001 Feb;43(1):1-11
references: 11148447
references: Mol Biol Cell. 2012 Jun;23(12):2362-72
references: 22535523
references: Wiley Interdiscip Rev Syst Biol Med. 2012 May-Jun;4(3):273-83
references: 22334480
references: J Hematol Oncol. 2014 Mar 05;7:19
references: 24598126
references: Cell. 2011 Mar 4;144(5):646-74
references: 21376230
references: Cancer Res. 2007 Feb 1;67(3):901-10
references: 17283120
references: Cell Adh Migr. 2012 Jul-Aug;6(4):365-73
references: 22796940
references: Cell. 2008 May 16;133(4):704-15
references: 18485877
references: Cancer Manag Res. 2014 Jul 02;6:291-300
references: 25061338
references: Ann Surg. 2013 Nov;258(5):775-82; discussion 782-3
references: 23989057
references: Cancer Res. 2008 Oct 1;68(19):7872-81
references: 18829543
references: Gastroenterology. 2013 Apr;144(4):799-807.e24
references: 23266556
references: Cell Signal. 2014 Mar;26(3):580-93
references: 24316392
references: BMC Cancer. 2012 Oct 19;12 :481
references: 23082988
references: PLoS One. 2013;8(3):e57692
references: 23520479
references: Mol Biol Cell. 2013 Nov;24(22):3569-76
references: 24025717
references: Biochim Biophys Acta. 2009 Dec;1796(2):75-90
references: 19306912
references: Genetics. 1996 Sep;144(1):249-54
references: 8878690
references: Int J Gynecol Cancer. 2014 Oct;24(8):1359-65
references: 25054447
references: J Natl Cancer Inst. 2004 Oct 6;96(19):1420-5
references: 15467030
references: J Invest Dermatol. 2013 May;133(5):1269-77
references: 23190890