title: Genetic diversity of human blastocystis isolates in khorramabad, central iran.
abstract: BACKGROUND
  There are some genetic differences in Blastocystis that show the existence of species or genotypes. One of these genes that help in identifying Blastocystis is SSUrRNA. The aim of this study was assessment of genetic diversity of Blastocystis by PCR with seven pairs of STS primers.
  
  
  METHODS
  This study was done on 511 stool samples collected from patients referred to the health care centers of Khorramabad, Central Iran, in 2012. Genomic DNA was extracted and in order to determine the Blastocystis subtype in contaminated samples, seven pairs of primers STS (subtype specific sequence-tagged site) were used.
  
  
  RESULTS
  Out of 511 samples, 33 (6.5%) samples were infected with Blastocystis. Subtype (ST) of 30 samples was identified and three subtypes 2, 3 and 4 were determined. Mix infection was reported 10% which 3.33% of the infection was for the mixture of ST 3 and ST5 and 6.67% was for the mixture of ST 2 and ST 3.
  
  
  CONCLUSIONS
  The predominant subtype was ST3 that is the main human subtype. The dominance of ST2 and 5 are important in this study. This superiority has been reported in some of the studies in ST 2 which is different from the studies in other countries, because they have announced priorities of the ST1 and ST6 after ST3.
authors: Dept. of Parasitology, Faculty Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Ebrahim
authors: E
authors: Badparva
authors: Dept. of Parasitology, Faculty Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Javid
authors: J
authors: Sadraee
authors: Dept. of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors: Farnaz
authors: F
authors: Kheirandish
authors: Dept. of Parasitology, Faculty Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Mehdi
authors: M
authors: Frouzandeh
keywords: Blastocystis; Iran; PCR; Subtype
references: Biol Rev Camb Philos Soc. 1998 Aug;73(3):203-66
references: 9809012
references: Parasitol Int. 2007 Dec;56(4):281-6
references: 17627869
references: J Parasitol. 2006 Oct;92(5):1081-7
references: 17152954
references: Parasitol Res. 2008 Apr;102(5):853-60
references: 18193282
references: Parasitol Res. 2007 Nov;101(6):1527-32
references: 17665214
references: Trends Parasitol. 2007 Mar;23(3):93-6
references: 17241816
references: Parasitol Res. 2006 Oct;99(5):597-601
references: 16688468
references: Mol Cell Probes. 1998 Jun;12(3):153-9
references: 9664577
references: Parasitol Res. 2011 Jul;109(1):205-12
references: 21210149
references: Eur J Clin Microbiol Infect Dis. 2012 Jul;31(7):1689-96
references: 22350386
references: Parasitol Res. 2009 Nov;105(6):1589-92
references: 19685075
references: Ann Trop Med Parasitol. 2008 Jan;102(1):85-8
references: 18186983
references: Microbiology. 2004 May;150(Pt 5):1147-51
references: 15133074
references: Parasitol Res. 2009 Aug;105(2):413-21
references: 19290540
references: Parasitol Res. 2012 Dec;111(6):2311-5
references: 22948205
references: Infect Genet Evol. 2012 Mar;12(2):263-73
references: 22116021
references: Clin Microbiol Rev. 2008 Oct;21(4):639-65
references: 18854485
references: J Parasitol. 2003 Jun;89(3):585-94
references: 12880261
references: Int J Parasitol. 2009 Mar;39(4):473-9
references: 18755193
references: J Eukaryot Microbiol. 1996 Mar-Apr;43(2):127-30
references: 8720942
references: Parasitol Res. 2008 Dec;104(1):85-93
references: 18795333
references: Parasitol Res. 2009 Oct;105(4):949-55
references: 19471964