title: Physicochemical properties of ternary oxovanadium(IV) complexes with oxydiacetate and 1,10-phenanthroline or 2,2'-bipyridine. Cytoprotective activity in hippocampal neuronal HT22 cells.
abstract: The aim of this work was to find a relationship between physicochemical properties of the oxovanadium(IV) complexes, namely [VO(ODA)(H2O)2], [VO(ODA)(phen)]·1.5H2O and [VO(ODA)(bipy)]·2H2O (ODA = oxydiacetate) as well as [VO(H2O)5](2+), and their biological activity. A potentiometric titration method has been used to characterize the stability of the complexes in aqueous solutions. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the NBT assay as well as a cyclic voltammetry (CV) technique. Additionally, the investigations of the antioxidant properties of the complexes were complemented by studying their reactivity towards organic radicals (the ABTS and DPPH tests). Finally, the biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Hippocampal neuronal cell line HT22 (the MTT and LDH tests). The obtained results showed that all the compounds under study display antioxidant properties but a concentration-depended protective effect against the oxidative damage was found for [VO(ODA)(bipy)]·2H2O only.
authors: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.
authors: Dariusz
authors: D
authors: Wyrzykowski
authors: Iwona
authors: I
authors: Inkielewicz-Stępniak
authors: Joanna
authors: J
authors: Pranczk
authors: Krzysztof
authors: K
authors: Żamojć
authors: Patrycja
authors: P
authors: Zięba
authors: Aleksandra
authors: A
authors: Tesmar
authors: Dagmara
authors: D
authors: Jacewicz
authors: Tadeusz
authors: T
authors: Ossowski
authors: Lech
authors: L
authors: Chmurzyński
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry; D012996:Solutions; D013481:Superoxides / Q000737:chemistry; D014638:Vanadates / Q000737:chemistry*
chemical_list: D000085:Acetates; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010618:Phenanthrolines; D012996:Solutions; D013481:Superoxides; C075743:oxovanadium IV; D014638:Vanadates; D015082:2,2'-Dipyridyl; C025205:1,10-phenanthroline