title: MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.
  
  
  METHODS
  The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was down-regulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.
  
  
  RESULTS
  MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.
  
  
  CONCLUSIONS
  Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation.
authors: Department of Stomatology, People's Hospital of Mianzhu, Mianzhu, Sichuan, China (mainland).
authors: Wanke
authors: W
authors: Lei
authors: New Era Stroke Care and Research Institute, The PLA Second Artillery General Hospital, Beijing, China (mainland).
authors: Yun-e
authors: YE
authors: Liu
authors: Department of Oncology, Third People's Hospital of Chengdu, Chengdu, Sichuan, China (mainland).
authors: Yuzhu
authors: Y
authors: Zheng
authors: Department of Centre for Disease Prevention and Control, Chengdu Military Region, Chengdu, Sichuan, China (mainland).
authors: Lin
authors: L
authors: Qu
mesh_terms: D000465:Algorithms; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D049109:Cell Proliferation; D002469:Cell Separation; D019295:Computational Biology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D018398:Homeodomain Proteins; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D014157:Transcription Factors; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1
references: BMC Public Health. 2010;10:196
references: 20398380
references: Int J Biol Sci. 2013;9(7):743-52
references: 23983607
references: J Biol Chem. 2011 Oct 21;286(42):36677-85
references: 21878637
references: Cell. 2012 Mar 16;148(6):1172-87
references: 22424228
references: Int J Biol Sci. 2013;9(7):680-92
references: 23904792
references: Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R121-32
references: 15809264
references: Gastroenterol Res Pract. 2013;2013:804128
references: 24204382
references: Cell Biochem Biophys. 2014 Sep;70(1):215-24
references: 24633485
references: Oncogene. 2010 Jul 29;29(30):4297-306
references: 20514023
references: Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7
references: 23552692
references: Cell Physiol Biochem. 2013;31(6):823-32
references: 23816858
references: Nat Rev Mol Cell Biol. 2009 Feb;10(2):126-39
references: 19165215
references: Histol Histopathol. 2011 Oct;26(10):1353-61
references: 21870338
references: J Clin Oncol. 2005 Jan 10;23(2):346-56
references: 15637397
references: Cell. 2003 Dec 26;115(7):787-98
references: 14697198
references: Int J Biol Sci. 2011;7(3):347-63
references: 21494432
references: Nucleic Acids Res. 2007 Jan;35(Database issue):D149-55
references: 17108354
references: J Cell Biochem. 2011 Sep;112(9):2508-17
references: 21557297
references: Nature. 2004 Sep 16;431(7006):350-5
references: 15372042
references: Methods Mol Biol. 2011;731:237-45
references: 21516412
references: Int J Mol Sci. 2013;14(6):10749-60
references: 23702845
references: Nature. 2008 Jan 10;451(7175):147-52
references: 18185580
references: Genes Chromosomes Cancer. 2009 Jul;48(7):569-82
references: 19396866
references: Nat Rev Genet. 2009 Oct;10(10):704-14
references: 19763153
references: Mol Biol Cell. 2007 Sep;18(9):3533-44
references: 17615296
references: Nucleic Acids Res. 2010 Jan;38(Database issue):D137-41
references: 19850714
references: J Oral Pathol Med. 2015 Oct;44(9):693-8
references: 25367287
references: Nat Genet. 2005 May;37(5):495-500
references: 15806104
references: Mayo Clin Proc. 2007 Jul;82(7):878-87
references: 17605971
references: Cancer Res. 2008 Jan 15;68(2):537-44
references: 18199550
references: Nat Cell Biol. 2008 May;10(5):593-601
references: 18376396
references: PLoS One. 2011;6(11):e27840
references: 22132151
references: Nucleic Acids Res. 2005;33(20):6566-78
references: 16314317
references: J Natl Cancer Inst. 1998 Nov 4;90(21):1626-36
references: 9811312
references: Cancer Cell. 2011 Feb 15;19(2):232-43
references: 21316602
references: CA Cancer J Clin. 2013 Jan;63(1):11-30
references: 23335087
references: Gynecol Oncol. 2010 Jan;116(1):117-25
references: 19854497
references: J Can Dent Assoc. 2008 Apr;74(3):269-72
references: 18387267
references: PLoS Biol. 2004 Nov;2(11):e363
references: 15502875
references: Nucleic Acids Res. 2013 Jul;41(Web Server issue):W159-64
references: 23703208
references: Nat Rev Cancer. 2006 Nov;6(11):857-66
references: 17060945
references: Cancer Biol Ther. 2010 Aug 1;10(3):219-22
references: 20592490
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
references: 17991681
references: Nat Med. 2013 Mar;19(3):313-21
references: 23396211
references: Mol Cell. 2001 Jun;7(6):1267-78
references: 11430829
references: CA Cancer J Clin. 2014 Jan-Feb;64(1):9-29
references: 24399786
references: J Cell Physiol. 2007 Nov;213(2):374-83
references: 17680632
references: Cell. 2009 Jan 23;136(2):215-33
references: 19167326
references: CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
references: 21296855
references: Genes Dev. 2006 Mar 1;20(5):515-24
references: 16510870
references: Cell Physiol Biochem. 2014;33(2):457-67
references: 24556800
references: Cell Oncol (Dordr). 2013 Oct;36(5):385-94
references: 23999873
references: Cell. 2001 Dec 28;107(7):823-6
references: 11779458
references: J Calif Dent Assoc. 2009 Nov;37(11):789-98
references: 19998655
references: Nat Rev Cancer. 2002 Jun;2(6):442-54
references: 12189386
references: Annu Rev Med. 2009;60:167-79
references: 19630570