title: Human periosteum cell osteogenic differentiation enhanced by ionic silicon release from porous amorphous silica fibrous scaffolds.
abstract: Current synthetic grafts for bone defect filling in the sinus can support new bone formation but lack the ability to stimulate or enhance osteogenic healing. To promote such healing, osteoblast progenitors such as human periosteum cells must undergo osteogenic differentiation. In this study, we tested the hypothesis that degradation of porous amorphous silica fibrous (PASF) scaffolds can enhance human periosteum cell osteogenic differentiation. Two types of PASF were prepared and evaluated according to their densities (PASF99, PASF98) with 99 and 98% porosity, respectively. Silicon (Si) ions were observed to rapidly release from both scaffolds within 24 h in vitro. PASF99 Si ion release rate was estimated to be nearly double that of PASF98 scaffolds. Mechanical tests revealed a lower compressive strength in PASF99 as compared with PASF98. Osteogenic expression analysis showed that PASF99 scaffolds enhanced the expression of activating transcription factor 4, alkaline phosphatase, and collagen (Col(I)α1, Col(I)α2). Scanning electron microscopy showed cellular and extracellular matrix (ECM) ingress into both scaffolds within 16 days and the formation of Ca-P precipitates within 85 days. In conclusion, this study demonstrated that PASF scaffolds enhance human periosteum cell osteogenic differentiation by releasing ionic Si, and structurally supporting cellular and ECM ingress.
authors: Department of Biomedical Sciences, Baylor College of Dentistry, Texas a & M Health Science Center, Dallas, Texas, 75246.|Department of Applied Prosthodontics, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, 8528588, Japan.|Material Science and Engineering Department, University of Texas at Arlington, Arlington, Texas, 76019.
authors: Tetsurou
authors: T
authors: Odatsu
authors: Department of Biomedical Sciences, Baylor College of Dentistry, Texas a & M Health Science Center, Dallas, Texas, 75246.
authors: Taha
authors: T
authors: Azimaie
authors: Material Science and Engineering Department, University of Texas at Arlington, Arlington, Texas, 76019.
authors: Megan F
authors: MF
authors: Velten
authors: Department of Biomedical Sciences, Baylor College of Dentistry, Texas a & M Health Science Center, Dallas, Texas, 75246.
authors: Michael
authors: M
authors: Vu
authors: VADM Joel T, Boone Chair of Health and Security Studies, U.S. Naval War College, Center for Naval Warfare Studies, Newport, Rhode Island, 02841.
authors: Mark B
authors: MB
authors: Lyles
authors: Center for Excellence in Hip Disorders, Texas Scottish Rite Hospital for Children, Dallas, Texas, 75219.|Orthopedic Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, 753908883.
authors: Harry K
authors: HK
authors: Kim
authors: Material Science and Engineering Department, University of Texas at Arlington, Arlington, Texas, 76019.
authors: Pranesh B
authors: PB
authors: Aswath
authors: Department of Biomedical Sciences, Baylor College of Dentistry, Texas a & M Health Science Center, Dallas, Texas, 75246.
authors: Venu G
authors: VG
authors: Varanasi
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D010521:Periosteum / Q000166:cytology*; D012825:Silicon; D054457:Tissue Scaffolds
chemical_list: D012825:Silicon
keywords: Runx2; Si; osteocalcin; periosteum cell; scaffold
references: Biomaterials. 2000 Dec;21(23):2347-59
references: 11055282
references: J Biomed Mater Res. 2001 May;55(2):151-7
references: 11255166
references: Stem Cells. 2001;19(3):180-92
references: 11359943
references: Clin Orthop Relat Res. 2002 Feb;(395):44-52
references: 11937865
references: Biomaterials. 2003 Jun;24(13):2161-75
references: 12699652
references: Tissue Eng. 2003;9 Suppl 1:S45-64
references: 14511470
references: Clin Implant Dent Relat Res. 2004;6(3):165-73
references: 15726851
references: J Orthop Sci. 2005;10(2):192-9
references: 15815868
references: Tissue Eng. 2005 Nov-Dec;11(11-12):1640-9
references: 16411809
references: Biotechniques. 2007 Mar;42(3):355-62
references: 17390542
references: J Mater Sci Mater Med. 2008 Apr;19(4):1565-73
references: 18299963
references: Biochim Biophys Acta. 2009 Feb;1790(2):110-8
references: 19007861
references: Acta Biomater. 2009 May;5(4):1284-93
references: 19010097
references: Clin Implant Dent Relat Res. 2009 Oct;11 Suppl 1:e7-13
references: 19220847
references: J Biomed Mater Res A. 2010 Feb;92(2):596-603
references: 19235208
references: Acta Biomater. 2009 Nov;5(9):3536-47
references: 19497391
references: J Orthop Trauma. 2010 Mar;24 Suppl 1:S36-40
references: 20182233
references: Clin Oral Implants Res. 2010 Mar;21(3):346-9
references: 20443793
references: Tissue Eng Part A. 2011 Apr;17(7-8):1083-97
references: 21091326
references: Indian J Orthop. 2011 Jan;45(1):15-22
references: 21221218
references: J Mater Sci Mater Med. 2011 Mar;22(3):647-56
references: 21287244
references: Clin Oral Implants Res. 2011 Dec;22(12):1366-71
references: 21382087
references: Acta Biomater. 2011 Jun;7(6):2355-73
references: 21421084
references: Clin Oral Implants Res. 2012 Jun;23(6):682-689
references: 21631596
references: Adv Mater. 2012 Feb 7;24(6):776-80
references: 22228389
references: Biomaterials. 2012 Jun;33(16):4044-58
references: 22381474
references: J Biomed Mater Res A. 2012 Nov;100(11):3020-8
references: 22707185
references: Biomacromolecules. 2012 Sep 10;13(9):2811-20
references: 22813212
references: J Oral Implantol. 2012 Aug;38(4):325-36
references: 22913306
references: Bone. 2013 May;54(1):141-50
references: 23360788
references: Int J Oral Maxillofac Implants. 2013 Jan-Feb;28(1):77-83
references: 23377050
references: J Biomed Mater Res A. 2013 Aug;101(8):2405-11
references: 23526767
references: Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2757-65
references: 23623093
references: Biomaterials. 2013 Nov;34(35):8887-98
references: 23958029
references: Spine (Phila Pa 1976). 1995 May 1;20(9):1055-60
references: 7631235