title: Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton.
abstract: The fungal plant pathogen Verticillium dahliae is the causal agent of vascular wilt, a disease that can seriously diminish cotton fiber yield. The pathogenicity mechanism and the identity of the genes that interact with cotton during the infection process still remain unclear. In this study, we investigated the low-pathogenic, non-microsclerotium-producing mutant vdpr3 obtained in a previous study from the screening of a T-DNA insertional library of the highly virulent isolate Vd080; the pathogenicity-related gene (VdPR3) in wild-type strain Vd080 was cloned. Knockout mutants (ΔVdPR3) showed lower mycelium growth and obvious reduction in sporulation ability without microsclerotium formation. An evaluation of carbon utilization in mutants and wild-type isolate Vd080 demonstrated that mutants-lacking VdPR3 exhibited decreased cellulase and amylase activities, which was restored in the complementary mutants (ΔVdPR3-C) to levels similar to those of Vd080. ΔVdPR3 postponed infectious events in cotton and showed a significant reduction in pathogenicity. Reintroduction of a functional VdPR3 copy into ΔVdPR3-C restored the ability to infect cotton plants. These results suggest that VdPR3 is a multifunctional gene involved in growth development, extracellular enzyme activity, and virulence of V. dahliae on cotton.
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Ya-Lin
authors: YL
authors: Zhang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Zhi-Fang
authors: ZF
authors: Li
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Zi-Li
authors: ZL
authors: Feng
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Hong-Jie
authors: HJ
authors: Feng
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Li-Hong
authors: LH
authors: Zhao
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
authors: Yong-Qiang
authors: YQ
authors: Shi
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China. xphu@nwsuaf.edu.cn.
authors: Xiao-Ping
authors: XP
authors: Hu
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China. heqinanyang@163.com.
authors: He-Qin
authors: HQ
authors: Zhu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000681:Amylases / Q000235:genetics / Q000378:metabolism; D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D003368:Gossypium / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D020172:Verticillium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005656:Fungal Proteins; C036483:T-DNA; D037521:Virulence Factors; D000681:Amylases; D002480:Cellulase
keywords: Biological characteristics; Extracellular enzyme; VdPR3; Verticillium dahliae; Virulence
references: Mol Plant Microbe Interact. 2011 Jan;24(1):129-42
references: 20839958
references: PLoS Pathog. 2011 Jul;7(7):e1002137
references: 21829347
references: Mol Plant Microbe Interact. 2009 Apr;22(4):402-10
references: 19271955
references: Mol Biotechnol. 2011 Nov;49(3):209-21
references: 21424547
references: Phytopathology. 2008 Aug;98(8):871-85
references: 18943205
references: Mol Plant Microbe Interact. 2013 Mar;26(3):278-86
references: 23051172
references: Nat Genet. 2007 Apr;39(4):561-5
references: 17353894
references: Plant Physiol. 2008 Feb;146(2):669-81
references: 18065558
references: Fungal Genet Biol. 2005 Jun;42(6):483-92
references: 15893252
references: Mol Plant Microbe Interact. 2012 Jul;25(7):964-75
references: 22414440
references: Mol Plant Pathol. 2006 Mar 1;7(2):71-86
references: 20507429
references: Annu Rev Phytopathol. 2009;47:39-62
references: 19385730
references: Curr Genet. 2005 Aug;48(2):109-16
references: 16003535
references: Fungal Genet Biol. 2006 Apr;43(4):283-94
references: 16488633
references: J Appl Microbiol. 2007 Oct;103(4):1184-96
references: 17897223
references: Fungal Genet Biol. 2003 Feb;38(1):54-62
references: 12553936
references: PLoS One. 2010 Dec 07;5(12):e15319
references: 21151869
references: Fungal Genet Biol. 2012 Apr;49(4):271-83
references: 22387367
references: Gene. 2013 Oct 25;529(2):307-16
references: 23891822
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Fungal Genet Biol. 2010 May;47(5):406-15
references: 20144723
references: Fungal Genet Biol. 2004 Nov;41(11):973-81
references: 15465386
references: Genomics. 1995 Feb 10;25(3):674-81
references: 7759102
references: PLoS One. 2014 Jun 13;9(6):e100046
references: 24927478
references: PLoS One. 2013 Oct 16;8(10):e77501
references: 24147009
references: Fungal Genet Biol. 2008 Dec;45(12):1525-32
references: 18951989