title: Calcium and magnesium concentrations in uterine fluid and blood serum during the estrous cycle in the bovine.
abstract: To investigate uterine and serum Ca(++) and Mg(++) variations during the estrous cycle in the bovine, 66 genital tracts and blood samples were collected from Urmia abattoir, Urmia, Iran. The phase of the estrous cycle was determined by examination of the structures present on ovaries and uterine tonicity. Of the collected samples, 17 were pro-estrus, 12 estrus, 14 metestrus and 23 diestrus. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The mean ± SEM concentration of serum Ca(++) in pro-estrus, estrus, metestrus and diestrus was 5.77 ± 0.69, 8.87 ± 1.83, 10.95 ± 1.52, 11.09 ± 1.08 mg dL(-1), and the mean concentration of uterine fluid Ca(++) was 4.40 ± 0.72, 3.15 ± 0.67, 5.89 ± 0.88, 8.63 ± 0.97 mg dL(-1), respectively. The mean concentration of serum Mg(++) in pro-estrus, estrus, metestrus and diestrus was 3.53 ± 0.30, 4.20 ± 0.52, 3.49 ± 0.38, 3.39 ± 0.29 mg dL(-1), and mean concentration of uterine fluid Mg(++) was 5.27 ± 0.42, 4.92 ± 0.60, 5.56 ± 0.30, 5.88 ± 0.36 mg dL(-1), respectively. The serum and uterine fluid Ca(++) in pro-estrus were significantly different from those of the metestrus and diestrus. In all stages of estrous cycle the mean concentration of serum Ca(++) was higher than that in the uterine fluid. The difference between serum and uterine fluid Ca(++) in estrus, metestrus and diestrus was significant. There was no significant difference between serum Mg(++) content nor was it different from uterine fluid Mg(++) content at any stages of estrous cycle. In all stages of estrous cycle the uterine fluid Mg(++) was higher than that of the serum. These results suggest that during the estrous cycle in the cow, Ca(++) is passively secreted in uterine fluids and is mostly dependent on blood serum Ca(++) variations but Mg(++) is secreted independently and does not follow variations in the serum concentrations.
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Sayed Mortaza
authors: SM
authors: Alavi-Shoushtari
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Siamak
authors: S
authors: Asri-Rezaie
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Roya
authors: R
authors: Abedizadeh
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Amir
authors: A
authors: Khaki
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Mozhgan
authors: M
authors: Pak
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Sajad
authors: S
authors: Alizadeh
keywords: Bovine; Calcium; Estrous cycle; Magnesium; Uterine fluid
references: J Dairy Sci. 1992 Jan;75(1):58-65
references: 1541742
references: Hum Reprod Update. 1995 Jan;1(1):63-72
references: 9080207
references: Nature. 1977 Aug 11;268(5620):534-6
references: 560629
references: J Reprod Immunol. 1983 Jan;5(1):1-15
references: 6834337
references: Biochim Biophys Acta. 1968 Jun 26;160(2):289-91
references: 5658137
references: Arch Biochem Biophys. 1976 Dec;177(2):499-507
references: 1015831
references: Am J Physiol Cell Physiol. 2006 Dec;291(6):C1193-7
references: 16885395
references: J Reprod Fertil Suppl. 1982;32:181-5
references: 6962852
references: J Dairy Sci. 1983 Sep;66(9):1854-62
references: 6685141
references: Biol Reprod. 1977 Jun;16(5):682-90
references: 861339
references: Biol Reprod Suppl. 1970;2:128-58
references: 12254592
references: J Reprod Fertil. 1982 Mar;64(2):409-20
references: 7069659
references: Theriogenology. 2000 Jan 15;53(2):619-26
references: 10735054
references: Anim Reprod Sci. 2008 May;105(3-4):302-10
references: 17459619
references: Vet Med Nauki. 1986;23(2):29-33
references: 3727378
references: J Reprod Fertil Suppl. 1993;48:143-55
references: 8145202
references: Cornell Vet. 1991 Jul;81(3):313-28
references: 1879144
references: Anim Reprod Sci. 2006 Nov;96(1-2):10-20
references: 16412593
references: Theriogenology. 2007 Sep 1;68(4):538-48
references: 17617447