title: YB-1 overexpression promotes a TGF-β1-induced epithelial-mesenchymal transition via Akt activation.
abstract: The Y-box binding protein-1 (YB-1) is a transcription/translation regulatory protein, and the expression thereof is associated with cancer aggressiveness. In the present study, we explored the regulatory effects of YB-1 during the transforming growth factor-β1 (TGF-β1)-induced epithelial-to-mesenchymal transition (EMT) in lung adenocarcinoma cells. Downregulation of YB-1 increased E-cadherin promoter activity, and upregulation of YB-1 decreased promoter activity, suggesting that the YB-1 level may be correlated with the EMT. TGF-β1 induced YB-1 expression, and TGF-β1 translocated cytosolic YB-1 into the nucleus. YB-1 overexpression promoted TGF-β1-induced downregulation of epithelial markers, upregulation of mesenchymal markers, and cell migration. Moreover, YB-1 overexpression enhanced the expression of E-cadherin transcriptional repressors via TGF-β1-induced Akt activation. Our findings afford new insights into the role played by YB-1 in the TGF-β1 signaling pathway.
authors: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Republic of Korea.
authors: Bin
authors: B
authors: Ha
authors: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Republic of Korea.
authors: Eun Byul
authors: EB
authors: Lee
authors: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Republic of Korea.
authors: Jun
authors: J
authors: Cui
authors: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Republic of Korea.
authors: Yosup
authors: Y
authors: Kim
authors: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Republic of Korea; Gachon Medical Research Institute, Gil Medical Center, Gachon University, Incheon, Republic of Korea. Electronic address: hhjang@gachon.ac.kr.
authors: Ho Hee
authors: HH
authors: Jang
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015854:Up-Regulation; D051840:Y-Box-Binding Protein 1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; E-cadherin; Epithelial-mesenchymal transition; TGF-β1; YB-1