title: Multi-target determination of organic ultraviolet absorbents in organism tissues by ultrasonic assisted extraction and ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and reliable method was developed for multi-target determination of 13 most widely used organic ultraviolet (UV) absorbents (including UV filters and UV stabilizers) in aquatic organism tissues. The organic UV absorbents were extracted using ultrasonic-assisted extraction, purified via gel permeation chromatography coupled with silica gel column chromatography, and determined by ultra-high performance liquid chromatography-tandem mass spectrometry. Recoveries of the UV absorbents from organism tissues mostly ranged from 70% to 120% from fish filet with satisfactory reproducibility. Method quantification limits were 0.003-1.0ngg(-1) dry weight (dw) except for 2-ethylhexyl 4-methoxycinnamate. This method has been applied to analysis of the UV absorbents in wild and farmed aquatic organisms collected from the Pearl River Estuary, South China. 2-Hydroxy-4-methoxybenzophenone and UV-P were frequently detected in both wild and farmed marine organisms at low ngg(-1)dw. 3-(4-Methylbenzylidene)camphor and most of the benzotriazole UV stabilizers were also frequently detected in maricultured fish. Octocrylene and 2-ethylhexyl 4-methoxycinnamate were not detected in any sample. This work lays basis for in-depth study about bioaccumulation and biomagnification of the UV absorbents in marine environment.
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: pengx@gig.ac.cn.
authors: Xianzhi
authors: X
authors: Peng
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Jiabin
authors: J
authors: Jin
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Chunwei
authors: C
authors: Wang
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Weihui
authors: W
authors: Ou
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Caiming
authors: C
authors: Tang
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000737:chemistry*; D002681:China; D002853:Chromatography, Liquid; D005399:Fishes; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D013473:Sunscreening Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014465:Ultrasonics; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D013473:Sunscreening Agents; D014874:Water Pollutants, Chemical
keywords: Organism tissue; Pearl River Estuary; Silica gel column chromatography; Ultra-high performance liquid chromatography–tandem mass spectrometry; Ultrasonic-assisted extraction; Ultraviolet absorbents