title: The viral transcription group determines the HLA class I cellular immune response against human respiratory syncytial virus.
abstract: The cytotoxic T-lymphocyte-mediated killing of virus-infected cells requires previous recognition of short viral antigenic peptides bound to human leukocyte antigen class I molecules that are exposed on the surface of infected cells. The cytotoxic T-lymphocyte response is critical for the clearance of human respiratory syncytial virus infection. In this study, naturally processed viral human leukocyte antigen class I ligands were identified with mass spectrometry analysis of complex human leukocyte antigen-bound peptide pools isolated from large amounts of human respiratory syncytial virus-infected cells. Acute antiviral T-cell response characterization showed that viral transcription determines both the immunoprevalence and immunodominance of the human leukocyte antigen class I response to human respiratory syncytial virus. These findings have clear implications for antiviral vaccine design.
authors: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain;
authors: Carolina
authors: C
authors: Johnstone
authors: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain;
authors: Elena
authors: E
authors: Lorente
authors: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain;
authors: Alejandro
authors: A
authors: Barriga
authors: §Department of Biology, Technion-Israel Institute of Technology, 32000 Haifa, Israel;
authors: Eilon
authors: E
authors: Barnea
authors: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain;
authors: Susana
authors: S
authors: Infantes
authors: ¶Unité d'Immunité Cellulaire Antivirale, Département d'Immunologie, Institut Pasteur, Paris Cedex 15, France;
authors: François A
authors: FA
authors: Lemonnier
authors: ‖Department of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905.
authors: Chella S
authors: CS
authors: David
authors: §Department of Biology, Technion-Israel Institute of Technology, 32000 Haifa, Israel;
authors: Arie
authors: A
authors: Admon
authors: From the ‡Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain; dlopez@isciii.es.
authors: Daniel
authors: D
authors: López
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D002457:Cell Extracts; D002460:Cell Line; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology; D008024:Ligands; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D020543:Proteome / Q000378:metabolism; D018113:Respiratory Syncytial Virus, Human / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic
chemical_list: D002457:Cell Extracts; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; D008024:Ligands; D010455:Peptides; D020543:Proteome
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