title: [Identification of miR-126 knockdown mouse and the change of blood glucose].
abstract: OBJECTIVE
  To detect the expression of miR-126 in different tissues and organs and the change of peripheral blood glucose in microRNA-126 knock down (miR-126 KD) mouse, and to explore the pathological significance.
  
  
  METHODS
  Total RNAs were isolated from twelve kinds of tissues and organs in wild-type mouse (WT) and miR-126 KD mouse respectively. Th en, the expression level of miR-126 was detected by real-time PCR assay. Th e levels of peripheral blood glucose and body weight of miR-126 KD mice were measured. Th e pathologic changes of pancreas and lung tissue were observed by HE staining.
  
  
  RESULTS
  Compared with the WT mice, the relative expression of miR-126 in spleen, liver, muscle and lung from the miR-126 KD mice were dramatically decreased respectively (P<0.05). The level of peripheral blood glucose in the miR-126 KD mouse increased significantly at seven week and sixteen week after the birth (P<0.05). HE staining showed that the pathological structure of pancreas and liver were abnormal. The body weight of miR-126 KD mice was increased obviously from thirteen week after birth (P<0.05).
  
  
  CONCLUSIONS
  Peripheral blood glucose levels in the miR-126 KD mouse were dramatically elevated, which might be related to the pathological changes in the structure of pancreas and liver. These results suggest that miR-126 may play an important role in the metabolism of blood glucose and the development of type 2 diabetes mellitus.
authors: Department of Immunology and Immunology Innovation Base of Postgraduate Education, Zunyi Medical College, Zunyi Guizhou 563000, China.
authors: Yan
authors: Y
authors: Hu
authors: Yongju
authors: Y
authors: Li
authors: Chao
authors: C
authors: Chen
authors: Shunfei
authors: S
authors: Zhu
authors: Mengmeng
authors: M
authors: Guo
authors: Sijing
authors: S
authors: Liu
authors: Jing
authors: J
authors: Zheng
authors: Nalin
authors: N
authors: Qin
authors: Lin
authors: L
authors: Xu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D001835:Body Weight; D003924:Diabetes Mellitus, Type 2; D055785:Gene Knockdown Techniques; D008099:Liver / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010179:Pancreas / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001786:Blood Glucose; C545201:MIRN126 microRNA, mouse; D035683:MicroRNAs