title: Heat shock activates splicing at latent alternative 5' splice sites in nematodes.
abstract: Pre-mRNA splicing is essential for the regulation of gene expression in eukaryotes and is fundamental in development and cancer, and involves the selection of a consensus sequence that defines the 5' splice site (5'SS). Human introns harbor multiple sequences that conform to the 5'SS consensus, which are not used under normal growth conditions. Under heat shock conditions, splicing at such intronic latent 5'SSs occurred in thousands of human transcripts, resulting in pre-maturely terminated aberrant proteins. Here we performed a survey of the C. elegans genome, showing that worm's introns contain latent 5'SSs, whose use for splicing would have resulted in pre-maturely terminated mRNAs. Splicing at these latent 5'SSs could not be detected under normal growth conditions, while heat shock activated latent splicing in a number of tested C. elegans transcripts. Two scenarios could account for the lack of latent splicing under normal growth conditions (i) Splicing at latent 5'SSs do occur, but the nonsense mRNAs thus formed are rapidly and efficiently degraded (e.g. by NMD); and (ii) Splicing events at intronic latent 5'SSs are suppressed. Here we support the second scenario, because, nematode smg mutants that are devoid of NMD-essential factors, did not show latent splicing under normal growth conditions. Hence, these experiments together with our previous experiments in mammalian cells, indicate the existence of a nuclear quality control mechanism, termed Suppression Of Splicing (SOS), which discriminates between latent and authentic 5'SSs in an open reading frame dependent manner, and allows splicing only at the latter. Our results show that SOS is an evolutionary conserved mechanism, probably shared by most eukaryotes.
authors: a Department of Genetics; The Hebrew University of Jerusalem ; Jerusalem , Israel.
authors: Yuval
authors: Y
authors: Nevo
authors: Joseph
authors: J
authors: Sperling
authors: Ruth
authors: R
authors: Sperling
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D018389:Codon, Nonsense; D005091:Exons; D049751:Genome, Helminth; D018869:Heat-Shock Response; D006358:Hot Temperature; D006801:Humans; D007438:Introns; D059365:Nonsense Mediated mRNA Decay; D016366:Open Reading Frames; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D022821:RNA Splice Sites; D013312:Stress, Physiological
chemical_list: D029742:Caenorhabditis elegans Proteins; D018389:Codon, Nonsense; D012322:RNA Precursors; D022821:RNA Splice Sites
keywords: 5′ splice site; 5′SS; NMD, nonsense mediated decay; PTC, premature termination codon; SOS, suppression of splicing; alternative splicing; heat shock; smg mutants; splicing regulation
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