title: Antimicrobial, antioxidant, and cytotoxic properties of vasicine acetate synthesized from vasicine isolated from Adhatoda vasica L.
abstract: Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. vasica using column chromatography. Vasicine acetate was obtained by acetylation of Vasicine. Vasicine acetate exhibited good zone of inhibition against bacteria: 10 mm against E. aerogenes, 10 mm against S. epidermidis, and 10 mm against P. aeruginosa. Vasicine acetate showed minimum inhibitory concentration values against bacteria: M. luteus (125 μg/mL), E. aerogenes (125 μg/mL), S. epidermidis (125 μg/mL), and P. aeruginosa (125 μg/mL). The radical scavenging activity of Vasicine acetate was the maximum at 1000 μg/mL (66.15%). The compound showed prominent cytotoxic activity in vitro against A549 lung adenocarcinoma cancer cell line. Quantification of Vasicine and Vasicine acetate by HPLC-DAD analysis showed their contents to be 0.2293% and 0.0156%, respectively, on dry weight basis of the leaves. Vasicine acetate could be probed further in drug discovery programme.
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia ; Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: V
authors: V
authors: Duraipandiyan
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors: N A
authors: NA
authors: Al-Dhabi
authors: Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: C
authors: C
authors: Balachandran
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia ; Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India ; Visiting Professor Programme, Deanship of Scientific Research, College of Science, King Saud University, Saudi Arabia.
authors: S
authors: S
authors: Ignacimuthu
authors: Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: C
authors: C
authors: Sankar
authors: Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India.
authors: K
authors: K
authors: Balakrishna
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D027902:Justicia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D011799:Quinazolines / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D011799:Quinazolines; C000601701:vasicine acetate; C019592:vasicine; C004931:1,1-diphenyl-2-picrylhydrazyl
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