title: Influence of ornithine decarboxylase antizymes and antizyme inhibitors on agmatine uptake by mammalian cells.
abstract: Agmatine (4-aminobutylguanidine), a dicationic molecule at physiological pH, exerts relevant modulatory actions at many different molecular target sites in mammalian cells, having been suggested that the administration of this compound may have therapeutic interest. Several plasma membrane transporters have been implicated in agmatine uptake by mammalian cells. Here we report that in kidney-derived COS-7 cell line, at physiological agmatine levels, the general polyamine transporter participates in the plasma membrane translocation of agmatine, with an apparent Km of 44 ± 7 µM and Vmax of 17.3 ± 3.3 nmol h(-1) mg(-1) protein, but that at elevated concentrations, agmatine can be also taken up by other transport systems. In the first case, the physiological polyamines (putrescine, spermidine and spermine), several diguanidines and bis(2-aminoimidazolines) and the polyamine transport inhibitor AMXT-1501 markedly decreased agmatine uptake. In cells transfected with any of the three ornithine decarboxylase antizymes (AZ1, AZ2 and AZ3), agmatine uptake was dramatically reduced. On the contrary, transfection with antizyme inhibitors (AZIN1 and AZIN2) markedly increased the transport of agmatine. Furthermore, whereas putrescine uptake was significantly decreased in cells transfected with ornithine decarboxylase (ODC), the accumulation of agmatine was stimulated, suggesting a trans-activating effect of intracellular putrescine on agmatine uptake. All these results indicate that ODC and its regulatory proteins (antizymes and antizyme inhibitors) may influence agmatine homeostasis in mammalian tissues.
authors: Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Medicina, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.
authors: Bruno
authors: B
authors: Ramos-Molina
authors: Andrés J
authors: AJ
authors: López-Contreras
authors: Ana
authors: A
authors: Lambertos
authors: Christophe
authors: C
authors: Dardonville
authors: Asunción
authors: A
authors: Cremades
authors: Rafael
authors: R
authors: Peñafiel
mesh_terms: D000376:Agmatine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D019556:COS Cells; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000494:pharmacology; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D019791:Guanidine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D014162:Transfection
chemical_list: C484011:AZIN1 protein, human; D002352:Carrier Proteins; D005663:Furans; D065108:Ornithine Decarboxylase Inhibitors; C520808:diguanidine; D013096:Spermine; D000376:Agmatine; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C546395:AZIN2 protein, human; D019791:Guanidine; D013095:Spermidine; D011700:Putrescine