title: Diagnostic values of vascular endothelial growth factor and epidermal growth factor receptor for benign and malignant hydrothorax.
abstract: BACKGROUND
  Hydrothorax, as one of the common complications of malignant tumors, still cannot be sensitively detected in clinical practice, thus requiring a sensitive, specific method for diagnosis. The aim of this study was to analyze the correlation between levels of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in patients with benign and malignant hydrothorax.
  
  
  METHODS
  The contents of VEGF in the pleural effusion and serum of the patients with malignant pleural effusion (n = 35) and benign pleural effusion (n = 30) were detected by double antibody sandwich enzyme linked immunosorbent assay. The gene copy number level of EGFR in pleural effusion was detected by fluorescence in situ hybridization (FISH). The points with the highest sensitivity and specificity were selected as the critical values to calculate the diagnostic value of the VEGF in pleural effusion and serum, and EGFR gene copy number in pleural effusion.
  
  
  RESULTS
  The contents of VEGF in pleural effusion and serum of patients with malignant hydrothorax were (384.91 ± 120.18), and (129.62 ± 46.35) ng/L, respectively, which were significantly higher than those of the patients with benign hydrothorax (207.97 ± 64.04), (63.49 ± 24.58) ng/L (P < 0.01). The sensitivity and specificity of detecting VEGF in pleural effusion were 80.0% and 96.7% (the boundary value was 297.06 ng/L), respectively for diagnosing benign and malignant hydrothorax. The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax. The diagnostic efficiencies of EGFR and VEGF in hydrothorax were similar. There was a significant correlation between EGFR and VEGF in hydrothorax (P < 0.01).
  
  
  CONCLUSIONS
  VEGF and EGFR play important roles in the formation of pleural effusion. VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions. It is feasible to detect the gene copy number of the pleural effusion cell mass EGFR by FISH technique. Joint detection can improve the diagnostic sensitivity.
authors: Yan
authors: Y
authors: Gu
authors: Min
authors: M
authors: Zhang
authors: Guo-Hua
authors: GH
authors: Li
authors: Jun-Zhen
authors: JZ
authors: Gao
authors: Liping
authors: L
authors: Guo
authors: Xiao-Juan
authors: XJ
authors: Qiao
authors: Li-Hong
authors: LH
authors: Wang
authors: Lan
authors: L
authors: He
authors: Mei-Ling
authors: ML
authors: Wang
authors: Li
authors: L
authors: Yan
authors: Department of Respiratory Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, China.
authors: Xiu-Hua
authors: XH
authors: Fu
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D066246:ErbB Receptors / Q000097:blood*; D005260:Female; D018628:Gene Dosage / Q000235:genetics; D006801:Humans; D006876:Hydrothorax / Q000097:blood*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D066246:ErbB Receptors
references: Expert Rev Mol Diagn. 2012 Sep;12(7):679-81
references: 23153235
references: Zhonghua Yi Xue Za Zhi. 2011 Mar 22;91(11):775-7
references: 21600106
references: Cancer Cytopathol. 2010 Oct 25;118(5):269-77
references: 20665655
references: J Cancer Res Clin Oncol. 2012 Sep;138(9):1541-9
references: 22538453
references: Nat Med. 2008 Sep;14(9):985-90
references: 18670422
references: Am J Respir Crit Care Med. 2000 Nov;162(5):1987-2001
references: 11069845
references: Lung Cancer. 2008 May;60(2):160-5
references: 18367287
references: J Mol Diagn. 2009 May;11(3):182-5
references: 19324991
references: Hum Pathol. 2009 Mar;40(3):356-65
references: 18976796
references: Clin Cancer Res. 2004 Dec 15;10(24):8195-203
references: 15623594
references: Differentiation. 2007 Nov;75(9):770-87
references: 17999740
references: Lung Cancer. 2008 Sep;61(3):328-39
references: 18304690
references: Cancer Res. 2008 Apr 1;68(7):2106-11
references: 18381415
references: Pneumonol Alergol Pol. 2002;70(5-6):258-64
references: 12518624
references: Cancer. 2014 May 1;120(9):1379-86
references: 24481719
references: Acta Cytol. 2007 Jul-Aug;51(4):679-80
references: 17718156
references: N Engl J Med. 2008 Jul 24;359(4):366-77
references: 18596266
references: J Clin Oncol. 2008 Feb 20;26(6):983-94
references: 18281673
references: Cancer Immunol Immunother. 2010 Feb;59(2):239-46
references: 19633845
references: Clin Chem. 2006 Oct;52(10):1833-42
references: 16423903
references: Biomaterials. 2014 Apr;35(12):3729-35
references: 24484673
references: Lung. 2005 May-Jun;183(3):185-95
references: 16078040