title: Peptide network for detection of tissue-remodeling enzyme in the prognosis of hepatocellular carcinoma.
abstract: In this work, a surface-confined peptide network that can exhibit distinct structural features under protease cleavage and electrochemical treatment is developed as a highly sensitive biosensor for clinical detection of kallikrein 6 (KLK6). KLK6 is a serine protease of the tissue-remodeling process determining the development of hepatocellular carcinoma (HCC). The peptide network, immobilized on the electrode surface by a Au-S bond, loses its integrity upon KLK6 cleavage and is removed from the electrode by electrochemical desorption of thiol groups, while the network without protease cleavage can remain attached by a few intact Au-S bonds. In this manner, distinct conductivity of the electrode surface in the presence/absence of protease can result in a large signal-to-background ratio, enabling KLK6 detection in clinical samples. The detected KLK6 abundance can manifest the correlation between up-regulated KLK6 activity and the progress of HCC. These results suggest a potential future use of this peptide network as a biosensor to provide diagnostic information for better administration of HCC.
authors: Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing 210008, China.
authors: Yue
authors: Y
authors: Yu
authors: Hao
authors: H
authors: Li
authors: Bin
authors: B
authors: Zhang
authors: Xiaoshu
authors: X
authors: Pan
authors: Xiaoli
authors: X
authors: Zhu
authors: Yitao
authors: Y
authors: Ding
authors: Genxi
authors: G
authors: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D018450:Disease Progression; D006801:Humans; D007610:Kallikreins / Q000032:analysis* / Q000378:metabolism; D008113:Liver Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D010455:Peptides / Q000378:metabolism*; D011379:Prognosis
chemical_list: D010455:Peptides; C103551:KLK6 protein, human; D007610:Kallikreins
keywords: electrochemical detection; extracellular matrix; hepatocellular carcinoma; kallikrein 6; peptide network