title: Protein profile of Acetobacter pasteurianus HSZ3-21.
abstract: Acetobacter pasteurianus plays an important role in the process of traditional vinegar production and is also essential for the fermentation of Zhenjiang aromatic vinegar. In this study, we utilized the proteomic approach to analyze the proteomic profile of A. pasteurianus HSZ3-21, and 258 proteins were successfully identified by MALDI-TOF-MS and database search. The hydropathy and GO analyse combined with COG results of the identified proteins revealed the molecular biological characteristics of A. pasteurianus proteins, that is, most proteins of A. pasteurianus were related to metabolic process, binding, catalytic or cellular response. Meanwhile, our results also showed that some proteins of A. pasteurianus may be responsible for acetic acid tolerance, thermotolerance, and stress response. Therefore, the identification of 258 proteins not only deciphers protein composition and functional classification of A. pasteurianus, but also provides useful information for improving quality of Zhenjiang aromatic vinegar.
authors: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China, yanziljh@163.com.
authors: Zhiyan
authors: Z
authors: Zhang
authors: Haile
authors: H
authors: Ma
authors: Yanhua
authors: Y
authors: Yang
authors: Li
authors: L
authors: Dai
authors: Keping
authors: K
authors: Chen
mesh_terms: D019342:Acetic Acid / Q000378:metabolism / Q000633:toxicity; D000091:Acetobacter / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D019342:Acetic Acid
references: Microbiology. 2012 Jan;158(Pt 1):229-39
references: 22016572
references: J Proteomics. 2012 Mar 16;75(6):1701-17
references: 22155126
references: PLoS One. 2011;6(6):e20063
references: 21695201
references: Appl Environ Microbiol. 2006 Jan;72(1):497-505
references: 16391084
references: J Mol Biol. 1982 May 5;157(1):105-32
references: 7108955
references: FEMS Microbiol Lett. 2004 Jun 15;235(2):315-22
references: 15183880
references: Curr Microbiol. 2010 Apr;60(4):280-6
references: 19924479
references: Biosci Biotechnol Biochem. 2002 Apr;66(4):777-83
references: 12036050
references: Int J Food Microbiol. 2006 Feb 1;106(2):209-12
references: 16214251
references: BMC Genomics. 2013 Aug 01;14:526
references: 23902333
references: Appl Environ Microbiol. 2001 Dec;67(12):5474-81
references: 11722895
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Proteomics. 2003 Mar;3(3):337-54
references: 12627387
references: J Biosci Bioeng. 2004;97(5):339-42
references: 16233640
references: Biotechnol Prog. 1997 Sep-Oct;13(5):519-23
references: 9336975
references: J Biosci Bioeng. 2002;94(2):140-7
references: 16233284
references: Arch Microbiol. 2000 Oct;174(4):217-24
references: 11081789