title: Identification and application of keto acids transporters in Yarrowia lipolytica.
abstract: Production of organic acids by microorganisms is of great importance for obtaining building-block chemicals from sustainable biomass. Extracellular accumulation of organic acids involved a series of transporters, which play important roles in the accumulation of specific organic acid while lack of systematic demonstration in eukaryotic microorganisms. To circumvent accumulation of by-product, efforts have being orchestrated to carboxylate transport mechanism for potential clue in Yarrowia lipolytica WSH-Z06. Six endogenous putative transporter genes, YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D24607g, YALI0D20108g and YALI0E32901g, were identified. Transport characteristics and substrate specificities were further investigated using a carboxylate-transport-deficient Saccharomyces cerevisiae strain. These transporters were expressed in Y. lipolytica WSH-Z06 to assess their roles in regulating extracellular keto acids accumulation. In a Y. lipolytica T1 line over expressing YALI0B19470g, α-ketoglutarate accumulated to 46.7 g·L(-1), whereas the concentration of pyruvate decreased to 12.3 g·L(-1). Systematic identification of these keto acids transporters would provide clues to further improve the accumulation of specific organic acids with higher efficiency in eukaryotic microorganisms.
authors: 1] School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China [2] Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Hongwei
authors: H
authors: Guo
authors: School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Peiran
authors: P
authors: Liu
authors: UMR1238 Microbiologie et Génétique Moléculaire, INRA/CNRS/AgroPan's Tech, CBAI, BP 01, 78850 Thiverval-Grignon, France.
authors: Catherine
authors: C
authors: Madzak
authors: 1] School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China [2] Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Guocheng
authors: G
authors: Du
authors: 1] School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China [2] Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Jingwen
authors: J
authors: Zhou
authors: 1] School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China [2] Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Jian
authors: J
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D018628:Gene Dosage; D005800:Genes, Fungal; D042541:Intracellular Space / Q000378:metabolism; D007651:Keto Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000302:isolation & purification; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors; D014158:Transcription, Genetic; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D007651:Keto Acids; D026901:Membrane Transport Proteins; D012331:RNA, Fungal
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