title: Expression pattern of parkin isoforms in lung adenocarcinomas.
abstract: The parkin gene has been shown to be genetically altered in a wide variety of human tumors including lung cancer. Although many parkin splice variants have been identified, to date, most of the studies have only been focused on originally cloned isoforms. In this work, for the first time, the expression profile of parkin isoforms in human lung adenocarcinomas has been analyzed to identify their involvement in lung cancer. Their contribution in some biological conditions, such as proteasomal degradation or mitophagy or cell death, has been analyzed in human lung cells. The expression profile of parkin isoforms has been investigated in paraffin-embedded samples of human lung adenocarcinomas by using Western blot analysis. Their expression has also been evaluated in human lung adenocarcinoma and in human normal bronchial epithelial cell lines following treatment with a proteasome inhibitor or mitochondrial depolarizing agent, or in serum starvation. Parkin proteins were detected on blot by using two antibodies, AbI and AbII, which recognize different domains of originally cloned parkin. Furthermore, parkin immunolocalization has been visualized in both cell lines by using immunofluorescence analysis. Results have shown that H1 and/or H5, H14, H4 and/or H8 and/or H17 and H3 and/or H12 isoforms are expressed in human lung adenocarcinomas. Some of them are also present in A549 cell line, whereas they are absent or faintly expressed in BEAS-2B cells. Furthermore, their expression changed after treatment. Human lung adenocarcinomas express different parkin isoforms, which might represent markers of malignancy and could be linked to specific biological functions.
authors: Department of Biomedical and Biotechnological Sciences, Section of Human Anatomy and Histology, University of Catania, Via S.Sofia, 87, 95123, Catania, Italy.
authors: Agata Grazia
authors: AG
authors: D'Amico
authors: Grazia
authors: G
authors: Maugeri
authors: Gaetano
authors: G
authors: Magro
authors: Lucia
authors: L
authors: Salvatorelli
authors: Filippo
authors: F
authors: Drago
authors: Velia
authors: V
authors: D'Agata
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020033:Protein Isoforms / Q000096:biosynthesis* / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein
references: Proc Natl Acad Sci U S A. 2012 Apr 17;109 (16):6283-8
references: 22460798
references: Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1638-43
references: 18230723
references: Neurobiol Dis. 2007 Jun;26(3):681-7
references: 17467279
references: Eur J Neurosci. 2000 Dec;12 (12 ):4181-94
references: 11122330
references: Trends Cell Biol. 2014 Jun;24(6):332-41
references: 24485851
references: J Biol Chem. 2011 Jun 3;286(22):19630-40
references: 21454557
references: Clin Cancer Res. 2004 Apr 15;10 (8):2720-4
references: 15102676
references: Biomed Res Int. 2014;2014:690796
references: 25136611
references: Nat Genet. 2000 Jul;25(3):302-5
references: 10888878
references: Nat Genet. 2010 Jan;42(1):77-82
references: 19946270
references: Mol Biol Cell. 2003 Nov;14 (11):4541-56
references: 12937272
references: Science. 2013 Apr 26;340(6131):471-5
references: 23620051
references: Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15145-50
references: 20696900
references: Cell Death Dis. 2014 Jul 03;5:e1313
references: 24991765
references: Nature. 1998 Apr 9;392(6676):605-8
references: 9560156
references: Oncogene. 2008 Oct 9;27(46):6002-11
references: 18574468
references: Mamm Genome. 2000 Jun;11(6):417-21
references: 10818204
references: Oncogene. 2007 Jan 11;26(2):298-307
references: 16819513
references: Curr Genomics. 2014 Jun;15(3):203-16
references: 24955028
references: Hum Mol Genet. 2009 Oct 15;18(20):3832-50
references: 19617636
references: Hum Mol Genet. 1999 Apr;8(4):567-74
references: 10072423
references: Neurogenetics. 2005 Dec;6(4):179-84
references: 16086186
references: Neurodegener Dis. 2014;13(2-3):118-21
references: 24008413
references: Neurogenetics. 2008 Jul;9(3):163-72
references: 18335262
references: Neurochem Res. 2004 Sep;29(9):1715-24
references: 15453267
references: Brain Res Mol Brain Res. 2000 Feb 22;75(2):345-9
references: 10686358
references: J Biol Chem. 2002 Dec 6;277(49):47870-7
references: 12364339
references: Nat Rev Mol Cell Biol. 2011 Jan;12(1):9-14
references: 21179058
references: Ann Neurol. 2006 Oct;60(4):389-98
references: 17068789
references: Hum Mol Genet. 2003 Oct 15;12 (20):2587-97
references: 12925569
references: Proc Natl Acad Sci U S A. 2003 May 13;100(10 ):5956-61
references: 12719539
references: J Mol Neurosci. 2013 Oct;51(2):503-13
references: 23900722
references: Hum Mol Genet. 2004 Aug 15;13(16):1745-54
references: 15198987
references: Int J Cancer. 2009 Nov 1;125(9):2029-35
references: 19585504
references: Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14503-8
references: 18799731
references: J Cell Biol. 2008 Dec 1;183(5):795-803
references: 19029340
references: PLoS Biol. 2010 Jan 26;8(1):e1000298
references: 20126261
references: J Cell Sci. 2003 Oct 1;116(Pt 19):4011-9
references: 12928331
references: Nat Cell Biol. 2009 Nov;11(11):1370-5
references: 19801972
references: Nat Rev Genet. 2006 Apr;7(4):306-18
references: 16543934
references: Cell. 2001 Jun 29;105(7):891-902
references: 11439185
references: Proc Natl Acad Sci U S A. 2008 May 13;105(19):7070-5
references: 18443288
references: Hum Mol Genet. 2004 Jan 1;13(1):117-35
references: 14645198