title: Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem.
abstract: Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.
authors: Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
authors: Jun
authors: J
authors: Shigeto
authors: Yoshitaka
authors: Y
authors: Itoh
authors: Sakie
authors: S
authors: Hirao
authors: Kaori
authors: K
authors: Ohira
authors: Koki
authors: K
authors: Fujita
authors: Yuji
authors: Y
authors: Tsutsumi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D009154:Mutation; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D063245:Plant Development; D018547:Plant Stems / Q000033:anatomy & histology* / Q000378:metabolism*; D021382:Protein Sorting Signals; D021381:Protein Transport; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D021382:Protein Sorting Signals; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D002482:Cellulose; D008031:Lignin; D010544:Peroxidases; C000597915:Prx2 protein, Arabidopsis; C000597914:Prx25 protein, Arabidopsis; C000597916:Prx71 protein, Arabidopsis; D002480:Cellulase; D005947:Glucose
keywords: Arabidopsis; knockout mutant; lignin biosynthesis; plant peroxidase