title: Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina.
abstract: High-voltage-activated calcium channels are hetero-oligomeric protein complexes that mediate multiple cellular processes, including the influx of extracellular Ca(2+), neurotransmitter release, gene transcription, and synaptic plasticity. These channels consist of a primary α(1) pore-forming subunit, which is associated with an extracellular α(2)δ subunit and an intracellular β auxiliary subunit, which alter the gating properties and trafficking of the calcium channel. The cellular localization of the α(2)δ(3) subunit in the mouse and rat retina is unknown. In this study using RT-PCR, a single band at ∼ 305 bp corresponding to the predicted size of the α(2)δ(3) subunit fragment was found in mouse and rat retina and brain homogenates. Western blotting of rodent retina and brain homogenates showed a single 123-kDa band. Immunohistochemistry with an affinity-purified antibody to the α(2)δ(3) subunit revealed immunoreactive cell bodies in the ganglion cell layer and inner nuclear layer and immunoreactive processes in the inner plexiform layer and the outer plexiform layer. α(2)δ(3) immunoreactivity was localized to multiple cell types, including ganglion, amacrine, and bipolar cells and photoreceptors, but not horizontal cells. The expression of the α(2)δ(3) calcium channel subunit to multiple cell types suggests that this subunit participates widely in Ca-channel-mediated signaling in the retina.
authors: Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.
authors: Luis
authors: L
authors: Pérez de Sevilla Müller
authors: Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|Department of Medicine, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.
authors: Allison
authors: A
authors: Sargoy
authors: Physiology, Genetics and Microbiology, University of Alicante, 03690, Alicante, Spain.
authors: Laura
authors: L
authors: Fernández-Sánchez
authors: Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.
authors: Allen
authors: A
authors: Rodriguez
authors: Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.
authors: Janelle
authors: J
authors: Liu
authors: Physiology, Genetics and Microbiology, University of Alicante, 03690, Alicante, Spain.
authors: Nicolás
authors: N
authors: Cuenca
authors: Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|Department of Medicine, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|CURE-Digestive Diseases Research Center, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095.|Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California, 90073.
authors: Nicholas
authors: N
authors: Brecha
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D009419:Nerve Tissue Proteins
keywords: amacrine cell; calcium channel; ganglion cell; photoreceptor; retina; α2δ3 subunit
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