title: The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella typhimurium infection.
abstract: Resistance is increasing to several critical antimicrobials used to treat Salmonella typhimurium infection, urging people to search for new antimicrobial agents. In this work, we reported the possibility of a potent antimicrobial peptide cathelicidin-BF found in the venom of the snake Bungarus fasciatus in treating Salmonella typhimurium infection. We tested its activity in biological fluids and in vivo using a mouse model of Salmonella typhimurium infection, and examined the effect of cathelicidin-BF on Salmonella invasion to epithelial cells. In addition, the biodistribution of cathelicidin-BF was evaluated by using in vivo optical imaging. The results revealed that cathelicidin-BF was unstable in gastrointestinal tract, but retained substantially active in murine serum. Cathelicidin-BF attenuated the clinical symptoms of Salmonella infected-mice, significantly reduced the number of internalized Salmonella and attenuated Salmonella-induced decreases in TER in epithelial cells. Our results provide a first indication for the potential of cathelicidin-BF as a novel therapeutic option for salmonellosis.
authors: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, 310058 Hangzhou, China.
authors: Xi
authors: X
authors: Xia
authors: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, 310058 Hangzhou, China.
authors: Lin
authors: L
authors: Zhang
authors: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, 310058 Hangzhou, China. Electronic address: yzwang321@zju.edu.cn.
authors: Yizhen
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology* / Q000627:therapeutic use; D058491:Bacterial Load; D004195:Disease Models, Animal; D004355:Drug Stability; D007413:Intestinal Mucosa / Q000187:drug effects / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000382:microbiology; D008297:Male; D051379:Mice; D012480:Salmonella Infections / Q000188:drug therapy / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects / Q000382:microbiology; D014018:Tissue Distribution; D054804:Cathelicidins
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins
keywords: Antimicrobial peptides; Cathelicidin-BF; Salmonella typhimurium