title: Methods to study tumor surveillance using tumor cell transplantation into genetically engineered mice.
abstract: When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice.
authors: Ludwig Boltzmann Institute for Cancer Research, Vienna, Austria.
authors: Eva
authors: E
authors: Bauer
authors: Agnieszka
authors: A
authors: Witalisz
authors: Birgit
authors: B
authors: Strobl
authors: Dagmar
authors: D
authors: Stoiber
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism; D045744:Cell Line, Tumor; D017690:Cell Transplantation / Q000379:methods*; D055786:Gene Knockout Techniques; D007157:Immunologic Surveillance; D051379:Mice; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation / Q000276:immunology* / Q000379:methods* / Q000473:pathology; D009369:Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology*
chemical_list: D015415:Biomarkers