title: In vitro viability of human periodontal ligament cells in green tea extract.
abstract: BACKGROUND
  Delayed replantation of avulsed teeth may be successful if the majority of periodontal ligament cells (PDL) survive. A proper transport medium is required when immediate replantation is not possible. Green tea extract (GTE) may be effective in preserving the cells because of its special properties.
  
  
  OBJECTIVE
  This study was done to evaluate the potential of GTE in periodontal ligament cells preservation.
  
  
  METHODS
  Fifty-four extracted human teeth with closed apices were randomly divided into three groups each with 18 teeth as follow: GTE, water (negative control), and Hank's balanced salt solution (HBSS) (positive control). The specimens were immersed in the media for 1, 3, and 15 hours at 4°C (n = 6) and treated with collagenase 1A for 45 minutes. Cell viability was determined using the trypan blue exclusion technique.
  
  
  METHODS
  Data were analyzed by one-way analysis of variance (ANOVA), post hoc Tukey and paired t-test at significance level of P < 0.05.
  
  
  RESULTS
  Means (standard deviation, SD) of viable cells in HBSS, water, and GTE were estimated 348.33 ± 88.49, 101 ± 14.18, and 310.56 ± 56.97 at 1 hours; 273.4 ± 44.80, 64.16 ± 16.44, and 310.2 ± 11.21 at 3 hours; and 373.72 ± 67.81, 14.41 ± 2.88 and 315.24 ± 34.48 at 15 hours; respectively. No significant differences were found between HBSS and GTE at all the time intervals. Both these solutions could preserve the cells more than water significantly.
  
  
  CONCLUSIONS
  GTE and HBSS were equally effective in preserving the cells and were significantly superior to water.
authors: Department of Pediatrics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
authors: Maryam
authors: M
authors: Ghasempour
authors: Department of Pharmacology and Physiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors: Ali Akbar
authors: AA
authors: Moghadamnia
authors: Department of Cellular and Molecular Research Centre, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors: Zeynab
authors: Z
authors: Abedian
authors: Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors: Mahdi Pour
authors: MP
authors: Amir
authors: Department of Cellular and Molecular Research Centre, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors: Farideh
authors: F
authors: Feizi
authors: Department of Pediatrics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
authors: Samane
authors: S
authors: Gharekhani
keywords: Avulsion; green tea; periodontal ligament cell; storage medium
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