title: Crystal Diagnostics MultiPath System™.
abstract: The Crystal Diagnostics MultiPath System™ provides rapid detection of Escherichia coli O157 in fresh raw ground beef, raw beeftrim, and spinach. The Crystal Diagnostics system combines patented Liquid Crystal technology with antibody-coated paramagnetic microspheres to selectively capture and detect E. coli O157 in food matrixes. This is the only liquid crystal-based biosensor commercially available for the detection of pathogens. The Crystal Diagnostics system expeditiously provides the sensitivity and accuracy of the U.S. Department of Agriculture Food Safety Inspection Service (USDA-FSIS) and the U.S. Food and Drug Administration Bacteriological Analytical Manual (FDA-BAM) methods for detecting as low as one CFU of E. coli O157 per 375 g of raw ground beef and raw beef trim, or 200 g of raw spinach. An internal inclusivity validation demonstrated detection of all 50 tested strains of . coli O157. The internal and independent laboratory tests demonstrate that the method is rapid and sensitive for detecting of E. coli O157 in fresh raw ground beef, beef trim, and spinach.
authors: Curtis H
authors: CH
authors: Stumpf
authors: Weidong
authors: W
authors: Zhao
authors: Brian
authors: B
authors: Bullard
authors: Christine
authors: C
authors: Ammons
authors: Karl I
authors: KI
authors: Devlin
authors: Gary D
authors: GD
authors: Niehaus
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation; D002417:Cattle; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology / Q000295:instrumentation*; D008460:Meat / Q000382:microbiology*; D018724:Spinacia oleracea / Q000382:microbiology*