title: Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription.
abstract: It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
authors: Department of Cell Biology, German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin, Germany.
authors: Özen
authors: Ö
authors: Sercan Alp
authors: Sibel
authors: S
authors: Durlanik
authors: Daniel
authors: D
authors: Schulz
authors: Mairi
authors: M
authors: McGrath
authors: Joachim R
authors: JR
authors: Grün
authors: Marcus
authors: M
authors: Bardua
authors: Koichi
authors: K
authors: Ikuta
authors: Evridiki
authors: E
authors: Sgouroudis
authors: René
authors: R
authors: Riedel
authors: Sandra
authors: S
authors: Zehentmeier
authors: Anja E
authors: AE
authors: Hauser
authors: Motokazu
authors: M
authors: Tsuneto
authors: Fritz
authors: F
authors: Melchers
authors: Koji
authors: K
authors: Tokoyoda
authors: Hyun-Dong
authors: HD
authors: Chang
authors: Andreas
authors: A
authors: Thiel
authors: Andreas
authors: A
authors: Radbruch
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D007156:Immunologic Memory / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015851:Interleukin-7 / Q000276:immunology; D019394:Ki-67 Antigen / Q000096:biosynthesis; D037181:Lectins, C-Type / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D016192:Resting Phase, Cell Cycle / Q000276:immunology*; D017154:Stromal Cells / Q000276:immunology*; D014158:Transcription, Genetic; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000096:biosynthesis; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007371:Interferon-gamma; D007376:Interleukin-2; D015851:Interleukin-7; D019394:Ki-67 Antigen; D037181:Lectins, C-Type; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D000099192:CD69 Antigens; C498355:Mki67 protein, mouse
keywords: Bone marrow; CD8 T cell; Gene expression; Interleukin-7; Memory cells
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