title: lspA gene of Mycobacterium tuberculosis co-transcribes with Rv1540 and induced by surface and acidic stress.
abstract: Lipoprotein signal peptidase, lspA (Rv1539), is the only known gene in mycobacterial genome for cleaving the signal sequence from prolipoprotein to form mature lipoprotein. It has been implicated in maintaining the virulence of Mycobacterium tuberculosis. The regulation of lspA had not been studied so far. Here, we identify a novel operon lspA-Rv1540 in M. tuberculosis. We detected co-transcription of the open reading frames of lspA-Rv1540 in in-vitro as well as in ex-vivo conditions. Analysis of the sequence upstream to lspA revealed a strong promoter activity that was shown to be induced significantly by surface stress and acidic environment.
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: rakeshvpci@gmail.com.
authors: Rakesh
authors: R
authors: Pathak
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: microknp@gmail.com.
authors: Nisha
authors: N
authors: Rathor
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: garima_kushal@yahoo.co.in.
authors: Kushal
authors: K
authors: Garima
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: sharmanaresh47@yahoo.com.
authors: Naresh Kumar
authors: NK
authors: Sharma
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: poojamedmicro@gmail.com.
authors: Pooja
authors: P
authors: Singh
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: mandirav@rediffmail.com.
authors: Mandira
authors: M
authors: Varma-Basil
authors: Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: bosemridula@yahoo.co.in.
authors: Mridula
authors: M
authors: Bose
mesh_terms: D000143:Acids / Q000494:pharmacology*; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D019751:Intramolecular Transferases / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009876:Operon / Q000187:drug effects / Q000235:genetics; D011401:Promoter Regions, Genetic; D013314:Stress, Mechanical; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013499:Surface Properties; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000143:Acids; D001426:Bacterial Proteins; D016282:Aspartic Acid Endopeptidases; C095652:signal peptidase II; D019751:Intramolecular Transferases; C096995:pseudouridine synthases
keywords: Mycobacterium tuberculosis; Regulation; lspA promoter