title: Mixed carbonates as useful substrates for a fluorogenic assay for lipases and esterases.
abstract: Mixed carbonates of 7-hydroxy-4-methylcoumarin were shown to be a new class of probe for fluorogenic assays. They are promising substrates for fingerprinting enzyme hydrolytic activity, and proved particularly useful because of the low level of nonspecific degradation and ease of synthesis. They are highly relevant for screening lipase and esterase libraries. These advantages make umbelliferyl carbonates highly suitable substrates for high-throughput screening. Moreover, we report the use of chiral fluorogenic carbonates as enantiopreference probes.
authors: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw (Poland).
authors: Anna
authors: A
authors: Zadlo
authors: Dominik
authors: D
authors: Koszelewski
authors: Filip
authors: F
authors: Borys
authors: Ryszard
authors: R
authors: Ostaszewski
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology; D002175:Candida / Q000201:enzymology; D002254:Carbonates / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D004950:Esterases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods; D006736:Horses; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013379:Substrate Specificity; D013552:Swine
chemical_list: D002254:Carbonates; D005456:Fluorescent Dyes; D004950:Esterases; D008049:Lipase
keywords: carbonates; enzyme catalysis; fluorescent probes; high-throughput screening; hydrolases