title: Acute and chronic local inflammatory reaction after implantation of different extracellular porcine dermis collagen matrices in rats.
abstract: Two cross-linked acellular porcine dermal collagen matrices (Permacol and NRX) were implanted into rats and the acute and chronic local inflammatory tissue reactions were investigated after 7, 14, 28, and 112 days. Both membranes were stable in vivo for up to 112 days. All investigated immune cell populations (CD68+ macrophages, CD163+ macrophages, T lymphocytes, MHC class II positive cells, mast cells, and NK cells) were present. Their amount decreased significantly over time compared to day 7 after implantation. A change from an acute to a chronic inflammation and an associated shift from proinflammatory M1-like to anti-inflammatory M2-like macrophages were observed. In the early phase there was a significant correlation of T cells to CD68+ (M1-like) macrophages, whereas in the chronic phase T lymphocytes were positively correlated with CD163+ (M2-like) macrophages. The material NRX showed an enhanced inflammatory reaction in comparison to Permacol possibly caused by material characteristics such as a twofold higher thickness of the membrane, roughness, and water absorption capacity. Nevertheless, a more pronounced regenerative process as, for example, indicated by nestin expression demonstrated its possible suitability for applications as wound repair material.
authors: Department of Medical Biochemistry and Molecular Biology, University Medical Center Greifswald, Greifswalder Straße 11c, 17495 Karlsburg, Germany.
authors: Silke
authors: 0000-0001-7706-3008
authors: S
authors: Lucke
authors: Department of Surgery, University Medical Center Greifswald, Ferdinand-Sauerbruch-Straße, 17475 Greifswald, Germany.
authors: Andreas
authors: A
authors: Hoene
authors: Department of Medical Biochemistry and Molecular Biology, University Medical Center Greifswald, Greifswalder Straße 11c, 17495 Karlsburg, Germany.
authors: Uwe
authors: 0000-0001-9368-8315
authors: U
authors: Walschus
authors: MBP Medical Biomaterial Products GmbH, Lederstraße 7, 19306 Neustadt-Glewe, Germany.
authors: Anette
authors: A
authors: Kob
authors: MBP Medical Biomaterial Products GmbH, Lederstraße 7, 19306 Neustadt-Glewe, Germany.
authors: Jens-Wolfgang
authors: JW
authors: Pissarek
authors: Department of Medical Biochemistry and Molecular Biology, University Medical Center Greifswald, Greifswalder Straße 11c, 17495 Karlsburg, Germany.
authors: Michael
authors: 0000-0001-8173-6578
authors: M
authors: Schlosser
mesh_terms: D062088:Acellular Dermis / Q000009:adverse effects*; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D002908:Chronic Disease; D003094:Collagen / Q000009:adverse effects*; D007249:Inflammation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D019736:Prostheses and Implants; D051381:Rats; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D001672:Biocompatible Materials; C422414:Permacol; D003094:Collagen
references: Br J Surg. 1998 Apr;85(4):444-60
references: 9607524
references: J Leukoc Biol. 1989 Dec;46(6):556-64
references: 2572659
references: Br J Plast Surg. 2005 Jun;58(4):518-32
references: 15897038
references: Nat Rev Immunol. 2005 Dec;5(12):953-64
references: 16322748
references: Hernia. 2005 Oct;9(3):269-76
references: 16136391
references: Clin Dermatol. 2007 Jan-Feb;25(1):9-18
references: 17276196
references: Biomaterials. 2007 Sep;28(25):3587-93
references: 17524477
references: Br J Surg. 2008 Apr;95(4):438-46
references: 18256994
references: J Wound Care. 2008 May;17(5):218-20, 222
references: 18546997
references: Acta Biomater. 2009 Jan;5(1):1-13
references: 18938117
references: Acta Biomater. 2009 Feb;5(2):776-84
references: 18951072
references: Biomaterials. 2009 Mar;30(8):1482-91
references: 19121538
references: Acta Biomater. 2010 Feb;6(2):676-83
references: 19751851
references: Nat Immunol. 2010 Oct;11(10):889-96
references: 20856220
references: J Mater Sci Mater Med. 2011 Apr;22(4):1015-26
references: 21455678
references: Semin Immunopathol. 2011 May;33(3):221-33
references: 21271251
references: Hernia. 2011 Jun;15(3):315-9
references: 21234626
references: Hernia. 2012 Feb;16(1):77-89
references: 21805341
references: Biomaterials. 2012 May;33(15):3792-802
references: 22386919
references: J Mater Sci Mater Med. 2013 Mar;24(3):761-71
references: 23314622
references: Plast Reconstr Surg. 2013 Apr;131(4):697-705
references: 23249980
references: Biomed Mater. 2013 Jun;8(3):035009
references: 23598370
references: MBio. 2013;4(3):e00264-13
references: 23781069
references: J Biomed Mater Res A. 2013 Sep;101(9):2679-88
references: 23427077
references: Am J Physiol Regul Integr Comp Physiol. 2010 May;298(5):R1173-87
references: 20219869
references: Br J Dermatol. 2000 Jul;143(1):59-65
references: 10886136
references: Int J Pharm. 2001 Jun 19;221(1-2):1-22
references: 11397563
references: Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3880-5
references: 15004274
references: Am J Surg. 2004 May;187(5A):11S-16S
references: 15147986
references: Burns. 2004 Aug;30(5):431-7
references: 15225907
references: J Cancer Res Clin Oncol. 1981;101(1):13-22
references: 6792209
references: J Histochem Cytochem. 1984 Feb;32(2):219-29
references: 6198355
references: Immunology. 1985 Mar;54(3):589-99
references: 3882559
references: Surgery. 1987 Aug;102(2):300-5
references: 3497461
references: Trends Immunol. 2004 Dec;25(12):677-86
references: 15530839