title: Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.
abstract: Fertilisation is the first step in embryonic development, and dynamic changes of key genes may potentially improve assisted reproduction techniques efficiency during this process. Here, we analysed genes that were differentially expressed between oocytes and zygotes and focused on cytokinesis-related genes. Plk1 and Dctn3 were identified as showing dramatic changes in expression during fertilisation and were suggested to play a key role in inducing aneuploidy in zygotes and 8-cell embryos. Moreover, we found that maternal Plk1 and Dctn3 were expressed at lower levels in in vitro matured oocytes, which may have contributed to the high ratio of resulting embryos with abnormal Plk1 and Dctn3 expression levels, thereby reducing the developmental competence of the resulting embryos. Furthermore, the overexpression of Dctn3 can silence Plk1 expression, which suggests a potential regulation mechanism. In conclusion, our present study showed that aberrant expression of Plk1 and Dctn3 increases embryo aneuploidy and developmental failure, particularly in in vitro matured oocytes. Our results facilitate a better understanding of the effects of oocyte maternal gene expression on embryonic development and can be used to improve the outcome of assisted reproduction techniques.
authors: Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
authors: Yong
authors: Y
authors: Fan
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China [3] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors: Hong-Cui
authors: HC
authors: Zhao
authors: Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
authors: Jianqiao
authors: J
authors: Liu
authors: Yunnan Key Laboratory of Primate Biomedical Research and Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming, 650500, China.
authors: Tao
authors: T
authors: Tan
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.
authors: Ting
authors: T
authors: Ding
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China [3] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors: Rong
authors: R
authors: Li
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors: Yue
authors: Y
authors: Zhao
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.
authors: Jie
authors: J
authors: Yan
authors: Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
authors: Xiaofang
authors: X
authors: Sun
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China [3] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors: Yang
authors: Y
authors: Yu
authors: 1] Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China [2] Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China [3] Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors: Jie
authors: J
authors: Qiao
mesh_terms: D000782:Aneuploidy; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D048749:Cytokinesis / Q000235:genetics; D000072159:Dynactin Complex; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015053:Zygote / Q000378:metabolism; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1; C000606362:DCTN3 protein, human
references: Curr Biol. 2011 Apr 26;21(8):651-7
references: 21497085
references: J Biol Chem. 2007 Jul 6;282(27):19355-64
references: 17449914
references: J Assist Reprod Genet. 2011 Jun;28(6):489-93
references: 21472419
references: Cell Div. 2008 Jan 23;3:4
references: 18215321
references: EMBO J. 2013 Apr 3;32(7):1023-35
references: 23455152
references: Genes Cells. 2010 Nov;15(11):1145-57
references: 20964796
references: Hum Reprod. 2006 Jul;21(7):1705-19
references: 16571642
references: Cancer Sci. 2008 Dec;99(12):2444-54
references: 19018766
references: Nature. 2013 Aug 29;500(7464):593-7
references: 23892778
references: Oncogene. 2013 Sep 26;32(39):4593-601
references: 23318447
references: Mol Biol Rep. 2012 May;39(5):6187-91
references: 22207182
references: J Cell Biol. 1996 Dec;135(6 Pt 2):1701-13
references: 8991084
references: Stem Cell Reports. 2014 Apr 8;2(4):491-506
references: 24749073
references: Fertil Steril. 1991 Jan;55(1):109-13
references: 1986950
references: PLoS One. 2013;8(2):e54511
references: 23408943
references: Oncogene. 2012 Aug 2;31(31):3597-606
references: 22120710
references: Hum Reprod. 2012 Jul;27(7):2146-59
references: 22532606
references: Nat Struct Mol Biol. 2013 Sep;20(9):1131-9
references: 23934149
references: Placenta. 2011 Sep;32 Suppl 3:S252-6
references: 21782239
references: Mutat Res. 1997 Aug 1;392(1-2):97-107
references: 9269334
references: Hum Genet. 2013 Sep;132(9):1001-13
references: 23620267
references: Semin Reprod Med. 2012 Aug;30(4):289-301
references: 22723010
references: Biol Reprod. 1984 Feb;30(1):1-11
references: 6320914
references: Reprod Biomed Online. 2009 Jun;18(6):777-83
references: 19490781
references: Cell. 2009 May 29;137(5):835-48
references: 19490893
references: Nat Methods. 2009 May;6(5):377-82
references: 19349980
references: J Cell Biol. 1996 Feb;132(4):617-33
references: 8647893
references: Cell. 2012 Mar 16;148(6):1293-307
references: 22424236
references: Hum Mol Genet. 2004 Jul 15;13(14):1461-70
references: 15150160
references: Mol Cancer Res. 2007 Sep;5(9):881-90
references: 17855657
references: Cytogenet Genome Res. 2005;111(3-4):250-5
references: 16192701
references: Fertil Steril. 2011 Apr;95(5):1624-8.e1-2
references: 21316045
references: Development. 2013 Sep;140(18):3719-30
references: 23981655
references: Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6888-93
references: 20348415
references: Fertil Steril. 2010 Aug;94(3):1152-3
references: 20580357
references: Fertil Steril. 2013 Mar 1;99(3):632-41
references: 23357453
references: Cell Mol Life Sci. 2014 Jul;71(14):2707-15
references: 24652479
references: Bioinformatics. 2010 Jan 1;26(1):136-8
references: 19855105
references: Fertil Steril. 2011 Jul;96(1):73-78.e1
references: 21645890
references: Reprod Biomed Online. 2010 Oct;21(4):552-9
references: 20800547
references: Hum Mol Genet. 2007 Oct 15;16 Spec No. 2:R203-8
references: 17911163
references: Fertil Steril. 2011 Jan;95(1):99-104
references: 20553786
references: Stem Cells Dev. 2014 Mar 15;23(6):563-75
references: 24261581
references: Reprod Biomed Online. 2002 Jul-Aug;5(1):17-21
references: 12470540
references: J Cell Sci. 2003 Dec 1;116(Pt 23):4727-38
references: 14600259
references: BMC Evol Biol. 2012;12:95
references: 22726940
references: Lancet. 1978 Aug 12;2(8085):366
references: 79723
references: J Biol Chem. 1995 Sep 22;270(38):22077-80
references: 7673179
references: Gamete Res. 1989 Jun;23(2):189-201
references: 2499525
references: J Cell Sci. 2001 Jul;114(Pt 13):2417-26
references: 11559750
references: Oncogene. 2010 Jul 15;29(28):4046-57
references: 20473324
references: Mol Aspects Med. 2014 Aug;38:54-85
references: 23796757
references: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20489-93
references: 21059955
references: Hum Reprod Update. 2013 Jan-Feb;19(1):52-66
references: 23054129
references: Chromosoma. 1998 Dec;107(6-7):424-9
references: 9914374
references: Cell Div. 2009 Jan 22;4:4
references: 19161615