title: Necrotizing Enteritis and Hyperammonemic Encephalopathy Associated With Equine Coronavirus Infection in Equids.
abstract: Equine coronavirus (ECoV) is a Betacoronavirus recently associated clinically and epidemiologically with emerging outbreaks of pyrogenic, enteric, and/or neurologic disease in horses in the United States, Japan, and Europe. We describe the pathologic, immunohistochemical, ultrastructural, and molecular findings in 2 horses and 1 donkey that succumbed to natural infection with ECoV. One horse and the donkey (case Nos. 1, 3) had severe diffuse necrotizing enteritis with marked villous attenuation, epithelial cell necrosis at the tips of the villi, neutrophilic and fibrinous extravasation into the small intestinal lumen (pseudomembrane formation), as well as crypt necrosis, microthrombosis, and hemorrhage. The other horse (case No. 2) had hyperammonemic encephalopathy with Alzheimer type II astrocytosis throughout the cerebral cortex. ECoV was detected by quantitative polymerase chain reaction in small intestinal tissue, contents, and/or feces, and coronavirus antigen was detected by immunohistochemistry in the small intestine in all cases. Coronavirus-like particles characterized by spherical, moderately electron lucent, enveloped virions with distinct peplomer-like structures projecting from the surface were detected by negatively stained transmission electron microscopy in small intestine in case No. 1, and transmission electron microscopy of fixed small intestinal tissue from the same case revealed similar 85- to 100-nm intracytoplasmic particles located in vacuoles and free in the cytoplasm of unidentified (presumably epithelial) cells. Sequence comparison showed 97.9% to 99.0% sequence identity with the ECoV-NC99 and Tokachi09 strains. All together, these results indicate that ECoV is associated with necrotizing enteritis and hyperammonemic encephalopathy in equids.
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA fgiannitti@yahoo.com.
authors: F
authors: F
authors: Giannitti
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: S
authors: S
authors: Diab
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: A
authors: A
authors: Mete
authors: Department of Veterinary Microbiology and Pathology and Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
authors: J B
authors: JB
authors: Stanton
authors: Loomis Basin Equine Medical Center, Loomis, CA, USA.
authors: L
authors: L
authors: Fielding
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: B
authors: B
authors: Crossley
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: K
authors: K
authors: Sverlow
authors: California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: S
authors: S
authors: Fish
authors: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: S
authors: S
authors: Mapes
authors: Idaho Equine Hospital, Nampa, ID, USA.
authors: L
authors: L
authors: Scott
authors: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: N
authors: N
authors: Pusterla
mesh_terms: D000818:Animals; D001483:Base Sequence; D001927:Brain Diseases / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017934:Coronavirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004751:Enteritis / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D018397:Equidae; D005243:Feces / Q000821:virology; D005260:Female; D006734:Horse Diseases / Q000473:pathology* / Q000821:virology; D006736:Horses; D022124:Hyperammonemia / Q000662:veterinary; D007421:Intestine, Small / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D009336:Necrosis / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
keywords: betacoronavirus; encephalopathy; enteritis; equine coronavirus; horse; hyperammonemia; immunohistochemistry; infectious disease