title: Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats.
abstract: Yupingfeng is a Chinese herbal compound used efficaciously to treat respiratory tract diseases. Total glucosides of Yupingfeng have been proven effective in anti-inflammation and immunoregulation. Nevertheless, the role of total extract of Yupingfeng (YTE) in pulmonary fibrosis (PF), a severe lung disease with no substantial therapies, remains unknown. Present study was conducted to elucidate the anti-fibrotic activity of YTE. The rat PF model was induced by intratracheal administration of bleomycin (BLM, 5 mg/kg), and YTE (12 mg/kg/d) was gavaged from the second day. At 14 and 28 days, the lungs were harvested and stained with H&E and Masson's trichrome. The content of hydroxyproline (HYP) and type I collagen (Col-I) were detected, while the protein expression of high-mobility group box 1 (HMGB1), transforming growth factor-beta 1 (TGF-β1), Col-I and α-smooth muscle actin (α-SMA) were analyzed by immunohistochemistry or Western blot. As observed, YTE treatment attenuated the alveolitis and fibrosis induced by BLM, reduced the loss of body weight and increase of lung coefficient. Meanwhile, YTE strongly decreased the levels of HYP and Col-I, and reduced the over-expression of HMGB1, TGF-β1, Col-I and α-SMA. In conclusion, YTE could ameliorate BLM-induced lung fibrosis by alleviating HMGB1 activity and TGF-β1 activation, suggesting therapeutic potential for PF.
authors: School of Pharmacy (Anhui Key Laboratory of Bioactivity of Natural Products), Anhui Medical University, Hefei 230032, China; Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors: Liucheng
authors: L
authors: Li
authors: Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
authors: Delin
authors: D
authors: Li
authors: Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
authors: Liang
authors: L
authors: Xu
authors: Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
authors: Ping
authors: P
authors: Zhao
authors: School of Pharmacy (Anhui Key Laboratory of Bioactivity of Natural Products), Anhui Medical University, Hefei 230032, China; The Second Affiliated Hospital of Anhui Medical University, Hefei 230012, China.
authors: Ziyu
authors: Z
authors: Deng
authors: School of Pharmacy (Anhui Key Laboratory of Bioactivity of Natural Products), Anhui Medical University, Hefei 230032, China; Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors: Xiaoting
authors: X
authors: Mo
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors: Ping
authors: P
authors: Li
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors: Lianwen
authors: L
authors: Qi
authors: School of Pharmacy (Anhui Key Laboratory of Bioactivity of Natural Products), Anhui Medical University, Hefei 230032, China. Electronic address: lijun@ahmu.edu.cn.
authors: Jun
authors: J
authors: Li
authors: Pharmaceutical Preparation Section (Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine (TCM-2009-202)), The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. Electronic address: gaojianayfy@163.com.
authors: Jian
authors: J
authors: Gao
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D024042:Collagen Type I / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal; D005260:Female; D024243:HMGB1 Protein / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy*; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000199:Actins; D001761:Bleomycin; D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; D006909:Hydroxyproline; D053773:Transforming Growth Factor beta1; C104200:Hbp1 protein, rat; C498862:Acta2 protein, rat; C532967:yupingfeng
keywords: Astragalus membranaceus; High-mobility group box 1; Pulmonary fibrosis; Total extract of Yupingfeng; Transforming growth factor-beta 1