title: Production of CoQ10 in fission yeast by expression of genes responsible for CoQ10 biosynthesis.
abstract: Coenzyme Q10 (CoQ10) is essential for energy production and has become a popular supplement in recent years. In this study, CoQ10 productivity was improved in the fission yeast Schizosaccharomyces pombe. Ten CoQ biosynthetic genes were cloned and overexpressed in S. pombe. Strains expressing individual CoQ biosynthetic genes did not produce higher than a 10% increase in CoQ10 production. In addition, simultaneous expression of all ten coq genes did not result in yield improvements. Genes responsible for the biosynthesis of p-hydroxybenzoate and decaprenyl diphosphate, both of which are CoQ biosynthesis precursors, were also overexpressed. CoQ10 production was increased by overexpression of Eco_ubiC (encoding chorismate lyase), Eco_aroF(FBR) (encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase), or Sce_thmgr1 (encoding truncated HMG-CoA reductase). Furthermore, simultaneous expression of these precursor genes resulted in two fold increases in CoQ10 production.
authors: a QOL division , Kaneka Corporation , Takasago , Japan.
authors: Daisuke
authors: D
authors: Moriyama
authors: Kouji
authors: K
authors: Hosono
authors: Makoto
authors: M
authors: Fujii
authors: Motohisa
authors: M
authors: Washida
authors: Hirokazu
authors: H
authors: Nanba
authors: Tomohiro
authors: T
authors: Kaino
authors: Makoto
authors: M
authors: Kawamukai
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10
keywords: Schizosaccharomyces pombe; coenzyme Q; isoprenoids; mevalonate; ubiquinone