title: Hydroxyaptite nanorods patterned ZrO2 bilayer coating on zirconium for the application of percutaneous implants.
abstract: Percutaneous implant requires a tight bond between the underlying dermis of skin and implant surface to prevent epithelial down-growth and infection, while fibroblasts play a key role in the skin-implant integration. In this work, nanorod-shaped hydroxyaptite (HA) with a mean diameter of 70 nm and length of 400 nm was hydrothermally grown on micro-arc oxidized (MAOed) Ca- and P-doped ZrO2 to form a bilayer coating. The hydrothermal formation mechanism of HA nanorods was explored, and the adsorption of total protein on the coating from α-MEM medium containing 10% fetal bovine serum was examined. Employing L-929 cells, the behaviors of fibroblasts on the bilayer coating, including adhesion and proliferation were evaluated together the polished Zr and as-MAOed ZrO2. The obtained results show that the HA nanorods nucleated on ZrO2 and grew at the expense of the doped Ca and P ions during the hydrothermal treatment (HT). The HA nanorods patterned coating enhanced protein absorption, and significantly improved the adhesion and proliferation of fibroblasts compared to the as-MAOed ZrO2 and polished Zr. It suggests that the HA nanorods/ZrO2 coated zirconium has a potential application for percutaneous implants to enhance the attachment of skin.
authors: State-key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors: Lan
authors: L
authors: Zhang
authors: State-key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: yonghan@mail.xjtu.edu.cn.
authors: Yong
authors: Y
authors: Han
authors: Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
authors: Guoxin
authors: G
authors: Tan
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D019736:Prostheses and Implants; D012867:Skin / Q000187:drug effects*; D013696:Temperature; D014867:Water / Q000494:pharmacology; D014961:X-Ray Diffraction; D015040:Zirconium / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; D014867:Water; D017886:Durapatite; D015040:Zirconium; C028541:zirconium oxide
keywords: Adhesion; Fibroblast; Formation mechanism; HA nanorod; Proliferation