title: Bioresorbable scaffolds for bone tissue engineering: optimal design, fabrication, mechanical testing and scale-size effects analysis.
abstract: Bone scaffolds for tissue regeneration require an optimal trade-off between biological and mechanical criteria. Optimal designs may be obtained using topology optimization (homogenization approach) and prototypes produced using additive manufacturing techniques. However, the process from design to manufacture remains a research challenge and will be a requirement of FDA design controls to engineering scaffolds. This work investigates how the design to manufacture chain affects the reproducibility of complex optimized design characteristics in the manufactured product. The design and prototypes are analyzed taking into account the computational assumptions and the final mechanical properties determined through mechanical tests. The scaffold is an assembly of unit-cells, and thus scale size effects on the mechanical response considering finite periodicity are investigated and compared with the predictions from the homogenization method which assumes in the limit infinitely repeated unit cells. Results show that a limited number of unit-cells (3-5 repeated on a side) introduce some scale-effects but the discrepancies are below 10%. Higher discrepancies are found when comparing the experimental data to numerical simulations due to differences between the manufactured and designed scaffold feature shapes and sizes as well as micro-porosities introduced by the manufacturing process. However good regression correlations (R(2) > 0.85) were found between numerical and experimental values, with slopes close to 1 for 2 out of 3 designs.
authors: UNIDEMI, Department of Mechanical and Industrial Engineering, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors: Pedro G
authors: PG
authors: Coelho
authors: Department of Biomedical Engineering, University of Michigan, MI 48109-2125, USA.
authors: Scott J
authors: SJ
authors: Hollister
authors: Department of Biomedical Engineering, University of Michigan, MI 48109-2125, USA.
authors: Colleen L
authors: CL
authors: Flanagan
authors: LAETA, IDMEC, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal. Electronic address: prfernan@dem.ist.utl.pt.
authors: Paulo R
authors: PR
authors: Fernandes
mesh_terms: D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology*; D008422:Materials Testing; D055595:Mechanical Phenomena; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
keywords: Biofabrication; Bone scaffolds; Homogenization; Mechanical testing; Multiscale models