title: Synthesis and characterization of a new Inonotus obliquus polysaccharide-iron(III) complex.
abstract: A new Inonotus obliquus polysaccharide-iron(III) complex (IOPS-iron) was synthesized and characterized. The preparation conditions of IOPS-iron(III) were optimized and the physicochemical properties were characterized by physicochemical methods, scanning electron microscopy (SEM), electron paramagnetic resonance (EPR) spectroscopy, fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy, respectively. The highest iron content of IOPS-iron(III) complex (19.40%) was obtained at the conditions: the ratio of IOPS and FeCl3 • 6H2O was 3:5 (w/w), the pH value of alkali solution was 10, the reaction temperature was 30 °C and the reaction time was 6h. The iron(III) was shown to be bound through the binding sites of the polysaccharide IOPS and it could form spatially separated iron centers on the polysaccharide backbone. IOPS-iron(III) complex was found to have good digestive availability and antioxidant activities in the in vitro assays, which suggested the IOPS-iron(III) complex might be used as a new iron supplement candidate.
authors: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
authors: Jia
authors: J
authors: Wang
authors: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China. Electronic address: chenhx@tju.edu.cn.
authors: Haixia
authors: H
authors: Chen
authors: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
authors: Yanwei
authors: Y
authors: Wang
authors: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
authors: Lisha
authors: L
authors: Xing
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000648:ultrastructure; D066244:Proton Magnetic Resonance Spectroscopy; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D014867:Water
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antioxidant properties; Digestive availability; EPR; FTIR; Inonotus obliquus polysaccharide-iron(III) complex; NMR