title: A comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus.
abstract: Salt loading (SL) and water deprivation (WD) are experimental challenges that are often used to study the osmotic circuitry of the brain. Central to this circuit is the supraoptic nucleus (SON) of the hypothalamus, which is responsible for the biosynthesis of the hormones, arginine vasopressin (AVP) and oxytocin (OXT), and their transport to terminals that reside in the posterior lobe of the pituitary. On osmotic challenge evoked by a change in blood volume or osmolality, the SON undergoes a function-related plasticity that creates an environment that allows for an appropriate hormone response. Here, we have described the impact of SL and WD compared with euhydrated (EU) controls in terms of drinking and eating behavior, body weight, and recorded physiological data including circulating hormone data and plasma and urine osmolality. We have also used microarrays to profile the transcriptome of the SON following SL and remined data from the SON that describes the transcriptome response to WD. From a list of 2,783 commonly regulated transcripts, we selected 20 genes for validation by qPCR. All of the 9 genes that have already been described as expressed or regulated in the SON by osmotic stimuli were confirmed in our models. Of the 11 novel genes, 5 were successfully validated while 6 were false discoveries.
authors: School of Clinical Sciences, University of Bristol, Bristol, United Kingdom;
authors: Michael P
authors: MP
authors: Greenwood
authors: Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Physiological Sciences, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropedica, Brazil;
authors: Andre S
authors: AS
authors: Mecawi
authors: Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
authors: See Ziau
authors: SZ
authors: Hoe
authors: Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
authors: Mohd Rais
authors: MR
authors: Mustafa
authors: Clinical Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland;
authors: Kory R
authors: KR
authors: Johnson
authors: Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Al Tarfa, Doha, Qatar;
authors: Ghada A
authors: GA
authors: Al-Mahmoud
authors: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
authors: Lucila L K
authors: LL
authors: Elias
authors: School of Physiology and Pharmacology, University Walk, Bristol, United Kingdom; and.
authors: Julian F R
authors: JF
authors: Paton
authors: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
authors: Jose
authors: J
authors: Antunes-Rodrigues
authors: Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
authors: Harold
authors: H
authors: Gainer
authors: School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
authors: David
authors: D
authors: Murphy
authors: School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; c.hindmarch@bristol.ac.uk.
authors: Charles C T
authors: 0000-0002-6271-758X
authors: CC
authors: Hindmarch
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D001810:Blood Volume; D001835:Body Weight; D004326:Drinking; D004435:Eating; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009994:Osmolar Concentration; D064587:Osmoregulation; D010121:Oxytocin / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage*; D013495:Supraoptic Nucleus / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D059467:Transcriptome; D014868:Water Deprivation
chemical_list: D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary; D001127:Arginine Vasopressin; D010121:Oxytocin
keywords: neuroendocrine; salt load; supraoptic nucleus; transcriptome; water restriction
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