title: Enzyme responsive mesoporous silica nanoparticles for targeted tumor therapy in vitro and in vivo.
abstract: This study reports a biocompatible controlled drug release system based on mesoporous silica nanoparticles (MSNs) for tumor microenvironment responsive drug delivery. It was fabricated by grafting phenylboronic acid conjugated human serum albumin (PBA-HSA) onto the surfaces of MSNs as a sealing agent, via an intermediate linker of a functional polypeptide, which was composed of two functional units: the polycation cell penetrating peptide (CPP) polyarginine, and matrix metalloproteinase 2 (MMP-2) substrate peptide. A series of characterizations confirmed that the system had been successfully constructed. In vitro tests proved that the anticancer drug loading system could efficiently induce cell apoptosis in vitro. More importantly, the in vivo tumor experiments confirmed that the anticancer loading system could efficiently inhibit tumor growth with minimal side effects.
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China. kaiyong_cai@cqu.edu.cn.
authors: Junjie
authors: J
authors: Liu
authors: Beilu
authors: B
authors: Zhang
authors: Zhong
authors: Z
authors: Luo
authors: Xingwei
authors: X
authors: Ding
authors: Jinghua
authors: J
authors: Li
authors: Liangliang
authors: L
authors: Dai
authors: Jun
authors: J
authors: Zhou
authors: Xiaojing
authors: X
authors: Zhao
authors: Jingya
authors: J
authors: Ye
authors: Kaiyong
authors: K
authors: Cai
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D017209:Apoptosis; D001897:Boronic Acids / Q000737:chemistry; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes / Q000737:chemistry*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D010455:Peptides / Q000737:chemistry; D010587:Phagocytosis; D012709:Serum Albumin / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001897:Boronic Acids; D004337:Drug Carriers; D004798:Enzymes; D010455:Peptides; D012709:Serum Albumin; C015462:polyarginine; D012822:Silicon Dioxide; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C010686:benzeneboronic acid