title: microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics.
abstract: The stem cell niche is thought to affect cell cycle quiescence, proliferative capacity, and communication between stem cells and their neighbors. How these activities are controlled is not completely understood. Here we define a microRNA family (miRs-103/107) preferentially expressed in the stem cell-enriched limbal epithelium that regulates and integrates these stem cell characteristics. miRs-103/107 target the ribosomal kinase p90RSK2, thereby arresting cells in G0/G1 and contributing to a slow-cycling phenotype. Furthermore, miRs-103/107 increase the proliferative capacity of keratinocytes by targeting Wnt3a, which enhances Sox9 and YAP1 levels and thus promotes a stem cell phenotype. This miRNA family also regulates keratinocyte cell-cell communication by targeting: (a) the scaffolding protein NEDD9, preserving E-cadherin-mediated cell adhesion; and (b) the tyrosine phosphatase PTPRM, which negatively regulates connexin 43-based gap junctions. We propose that such regulation of cell communication and adhesion molecules maintains the integrity of the stem cell niche ultimately preserving self-renewal, a hallmark of epithelial stem cells.
authors: Department of Dermatology, Northwestern University, Chicago, Illinois, USA.
authors: Han
authors: H
authors: Peng
authors: Jong Kook
authors: JK
authors: Park
authors: Julia
authors: J
authors: Katsnelson
authors: Nihal
authors: N
authors: Kaplan
authors: Wending
authors: W
authors: Yang
authors: Spiro
authors: S
authors: Getsios
authors: Robert M
authors: RM
authors: Lavker
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D017629:Gap Junctions / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D016850:Limbus Corneae / Q000166:cytology; D008297:Male; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D060509:Wnt3A Protein / Q000494:pharmacology
chemical_list: D015820:Cadherins; C544749:MIRN103 microRNA, human; C555364:MIRN103 microRNA, mouse; C549045:MIRN107 microRNA, human; C555365:MIRN107 microRNA, mouse; D035683:MicroRNAs; D060509:Wnt3A Protein; D038744:Ribosomal Protein S6 Kinases, 90-kDa
keywords: Adherens junctions; Cell cycle; Cell-cell communication; Gap junctions; Proliferative capacity; Wnt signaling
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