title: Cryptosporidium huwi n. sp. (Apicomplexa: Eimeriidae) from the guppy (Poecilia reticulata).
abstract: The morphological, biological, and molecular characteristics of Cryptosporidium piscine genotype 1 from the guppy (Poecilia reticulata) are described, and the species name Cryptosporidium huwi n. sp. is proposed to reflect its genetic and biological differences from gastric and intestinal Cryptosporidium species. Oocysts of C.huwi n. sp. over-lap in size with Cryptosporidium molnari, measuring approximately 4.4-4.9 µm (mean 4.6) by 4.0-4.8 µm (mean 4.4 µm) with a length to width ratio of 1.04 (0.92-1.35) (n = 50). Similar to C.molnari, C.huwi n. sp. was identified in the stomach only and clusters of oogonial and sporogonial stages were identified deep within the epithelium. However, phylogenetic analysis of 18S rRNA sequences indicated that C. huwi n. sp. exhibited 8.5-9.2% and 3.5% genetic distance from C.molnari isolates and piscine genotype 7 respectively. At the actin locus, the genetic distance between C.huwi n. sp. and C.molnari was 16.6%. The genetic distance between C.huwi n. sp. and other Cryptosporidium species at the 18S locus was 13.2%-17% and at the actin locus was 18.9%-26.3%. Therefore C. huwi n. sp. is genetically distinct from previously described Cryptosporidium species.
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia. Electronic address: una.ryan@murdoch.edu.au.
authors: Una
authors: U
authors: Ryan
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Andrea
authors: A
authors: Paparini
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Kaising
authors: K
authors: Tong
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Rongchang
authors: R
authors: Yang
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Susan
authors: S
authors: Gibson-Kueh
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Amanda
authors: A
authors: O'Hara
authors: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia 6150, Australia.
authors: Alan
authors: A
authors: Lymbery
authors: Public Health Services, U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors: Lihua
authors: L
authors: Xiao
mesh_terms: D000818:Animals; D001483:Base Sequence; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D005838:Genotype; D010802:Phylogeny; D011036:Poecilia / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D013270:Stomach / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: 18S rRNA; Actin gene; Cryptosporidium huwi n. sp; Genetic characterization; Morphology; Phylogeny