title: Tissue non-specific alkaline phosphatase production by human dental pulp stromal cells is enhanced by high density cell culture.
abstract: The cell surface hydrolase tissue non-specific alkaline phosphatase (TNAP) (also known as MSCA-1) is used to identify a sub-population of bone marrow stromal cells (BMSCs) with high mineralising potential and is found on subsets of cells within the dental pulp. We aim to determine whether TNAP is co-expressed by human dental pulp stromal cells (hDPSCs) alongside a range of BMSC markers, whether this is an active form of the enzyme and the effects of culture duration and cell density on its expression. Cells from primary dental pulp and culture expanded hDPSCs expressed TNAP. Subsequent analyses revealed persistent TNAP expression and co-expression with BMSC markers such as CD73 and CD90. Flow cytometry and biochemical assays showed that increased culture durations and cell densities enhanced TNAP expression by hDPSCs. Arresting the hDPSC cell cycle also increased TNAP expression. These data confirm that TNAP is co-expressed by hDPSCs together with other BMSC markers and show that cell density affects TNAP expression levels. We conclude that TNAP is a potentially useful marker for hDPSC selection especially for uses in mineralised tissue regenerative therapies.
authors: Department of Oral Biology, School of Dentistry, University of Leeds, Leeds, LS9 7TF, UK, m.tomlinson@leeds.ac.uk.
authors: Matthew J
authors: MJ
authors: Tomlinson
authors: Caitriona
authors: C
authors: Dennis
authors: Xuebin B
authors: XB
authors: Yang
authors: Jennifer
authors: J
authors: Kirkham
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000032:analysis / Q000378:metabolism; D055815:Young Adult
chemical_list: D018800:Thy-1 Antigens; C548267:ALPL protein, human; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase
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