title: Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications.
abstract: To improve cardiovascular implant success, metal-based stents are designated to modulate endothelial cells adhesion and migration in order to prevent restenosis and late thrombosis diseases. Biomimetic coatings with extra-cellular matrix adhesive biomolecules onto stents surfaces are a strategy to recover a healthy endothelium. However, the appropriate bioactive sequences to selective promote growth of endothelium and the biomolecules surface immobilization strategy remains to be elucidated. In this study, biofunctionalization of cobalt chromium, CoCr, alloy surfaces with elastin-like recombinamers, ELR, genetically modified with an REDV sequence, was performed to enhance metal surfaces endothelialization. Moreover, physical adsorption and covalent bonding were used as biomolecules binding strategies onto CoCr alloy. Surfaces were activated with plasma and etched with sodium hydroxide previous to silanization with 3-chloropropyltriethoxysilane and functionalized with the ELR. CoCr alloy surfaces were successfully biofunctionalized and the use of an ELR with an REDV sequence, allows conferring bioactivity to the biomaterials surface, demonstrating a higher cell adhesion and spreading of HUVEC cells on the different CoCr surfaces. This effect is emphasized as increases the amount of immobilized biomolecules and directly related to the immobilization technique, covalent bonding, and the increase of surface charge electronegativity. Our strategy of REDV elastin-like recombinamers immobilization onto CoCr alloy surfaces via covalent bonding through organosilanes provides a bioactive surface that promotes endothelial cell adhesion and spreading.
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna 11, Ed. CEEI, 50118 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: maria.isabel.castellanos@upc.edu.
authors: Maria Isabel
authors: MI
authors: Castellanos
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: annesophie.zenses@gmail.com.
authors: Anne-Sophie
authors: AS
authors: Zenses
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: agraugar@gmail.com.
authors: Anna
authors: A
authors: Grau
authors: Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna 11, Ed. CEEI, 50118 Zaragoza, Spain; GIR BIOFORGE (Group for Advanced Materials and Nanobiotechnology), Universidad de Valladolid (UVA), Valladolid 47011, Spain. Electronic address: cabello@bioforge.uva.es.
authors: Jose Carlos
authors: JC
authors: Rodríguez-Cabello
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna 11, Ed. CEEI, 50118 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: francesc.xavier.gil@upc.edu.
authors: Francisco Javier
authors: FJ
authors: Gil
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna 11, Ed. CEEI, 50118 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: jose.maria.manero@upc.edu.
authors: Jose María
authors: JM
authors: Manero
authors: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna 11, Ed. CEEI, 50118 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE), UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: marta.pegueroles@upc.edu.
authors: Marta
authors: M
authors: Pegueroles
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D002319:Cardiovascular System / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002858:Chromium Alloys / Q000494:pharmacology*; D004549:Elastin / Q000737:chemistry* / Q000494:pharmacology*; D004727:Endothelium / Q000187:drug effects*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005453:Fluorescence; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007526:Isoelectric Point; D018625:Microscopy, Atomic Force; D009842:Oligopeptides / Q000494:pharmacology; D056951:Photoelectron Spectroscopy; D055672:Static Electricity; D013816:Thermodynamics; D017155:Wettability
chemical_list: D002858:Chromium Alloys; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004549:Elastin; D016650:Fluorescein-5-isothiocyanate
keywords: Biomimetic materials; CoCr alloy; Elastin-like recombinamers; Endothelialization; Surface functionalization