title: Aging of adhesive interfaces treated with benzalkonium chloride and benzalkonium methacrylate.
abstract: Inhibition of endogenous dentin matrix metalloproteinases (MMPs) within incompletely infiltrated hybrid layers can contribute to the preservation of resin-dentin bonds. This study evaluated the bond stability of interfaces treated with benzalkonium chloride (BAC) and benzalkonium methacrylate (MBAC), and the inhibitory properties of these compounds on dentin MMP activity. Single-component adhesive ALL-BOND UNIVERSAL, modified with BAC or MBAC at concentrations of 0, 0.5, 1.0, and 2.0%, was used for microtensile bond strength (μTBS) evaluation after 24 h, 6 months, and 1 yr. Beams produced from human dentin were treated with 37% phosphoric acid, dipped in 0.5% BAC, 1.0% BAC, or water (control) for 60 s, and then incubated in SensoLyte generic MMP substrate to determine MMP activity. A significant decrease in the μTBS after 6 months and 1 yr was observed for the control group only. No significant differences among groups were shown at 24 h. After 6 months and 1 yr, the control group demonstrated significantly lower μTBS than all treatment groups. When applied for 60 s, 0.5% BAC inhibited total MMP activity by 31%, and 1.0% BAC inhibited total MMP activity by 54%. Both BAC and MBAC contributed to the preservation of resin-dentin bonds, probably because of their inhibitory properties of endogenous dentin proteinases.
authors: Department of Restorative Dentistry, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA.
authors: Camila
authors: C
authors: Sabatini
authors: David H
authors: DH
authors: Pashley
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D001548:Benzalkonium Compounds / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003804:Dentin / Q000201:enzymology / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry; D020782:Matrix Metalloproteinases / Q000032:analysis; D008689:Methacrylates / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
chemical_list: C074864:All-Bond system; D001548:Benzalkonium Compounds; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; D010756:Phosphoric Acids; C030242:phosphoric acid; D020782:Matrix Metalloproteinases
keywords: benzalkonium chloride; bond strength; dental adhesive; dentin; matrix metalloproteinase
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