title: [Juglone inhibits proliferation and induces apoptosis of human cervical squamous cancer SiHa cells].
abstract: OBJECTIVE
  To explore the effect of juglone on proliferation and apoptosis of human cervical squamous cancer SiHa cells.
  
  
  METHODS
  Cultured SiHa cells in the exponential growth phase were grouped into blank control group and 10, 20, 50, 80 and 100 μmol/L juglone treatment groups. Methyl thiazolyl tetrazolium (MTT) assay was adopted to observe the inhibitory effect of juglone on the proliferation of SiHa cells, and then 50% inhibitory concentration (IC50) was calculated through formula. Annexin V-FITC/PI double staining and flow cytometry were used to detect the effect of 20 μmol/L juglone on SiHa cell apoptosis. Western blot was applied to determine the expressions of Bcl-2 and Bax.
  
  
  RESULTS
  MTT assay showed that, compared with the control group, treatment groups all showed significant inhibitory effects on SiHa cell growth, and IC50 was 20.4 μmol/L. Flow cytometry demonstrated that early apoptosis rate of SiHa cells in the control group was (2.46 ± 0.37)%, and after treatment with 20 μmol/L Juglone for 12 hours, the apoptosis rate was raised to (18.47 ± 2.26)%; Western blot analysis showed that the expression of Bcl-2 decreased while the expression of Bax increased significantly in SiHa cells treated with 20 μmol/L juglone.
  
  
  CONCLUSIONS
  Juglone could significantly inhibit the proliferation and induce the apoptosis of SiHa cells.
authors: Jilin Medical College, Jilin 132013, China.
authors: Wei
authors: W
authors: Zhang
authors: Jilin Medical College, Jilin 132013, China.
authors: Yan
authors: Y
authors: Li
authors: Jilin Medical College, Jilin 132013, China.
authors: Jun
authors: J
authors: Luo
authors: Jilin Medical College, Jilin 132013, China.
authors: Xiaojing
authors: X
authors: Lu
authors: Jilin Medical College, Jilin 132013, China.
authors: Moran
authors: M
authors: Chen
authors: Jilin Medical College, Jilin 132013, China.
authors: Wenhe
authors: W
authors: Zhu
authors: Jilin Medical College, Jilin 132013, China.
authors: Yanxia
authors: Y
authors: Jiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009285:Naphthoquinones / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D009285:Naphthoquinones; D051028:bcl-2-Associated X Protein; C005134:juglone