title: Cysteine dioxygenase type 1 promotes adipogenesis via interaction with peroxisome proliferator-activated receptor gamma.
abstract: Mammalian cysteine dioxygenase type 1 (CDO1) is an essential enzyme for taurine biosynthesis and the biodegradation of toxic cysteine. As previously suggested, Cdo1 may be a marker of liposarcoma progression and adipogenic differentiation, but the role of Cdo1 in adipogenesis has yet been reported. In this study, we found that the expression of Cdo1 is dramatically elevated during adipogenic differentiation of 3T3-L1 pre-adipocytes and mouse bone marrow-derived mesenchymal stem cells (mBMSCs). Conversely, knockdown of Cdo1 inhibited expression of adipogenic specific genes and lipid droplet formation in 3T3-L1 cells and mBMSCs. Mechanistically, we found Cdo1 interacted with Pparγ in response to adipogenic stimulus. Further, depletion of Cdo1 reduced the recruitment of Pparγ to the promoters of C/EBPα and Fabp4. Collectively, our finding indicates that Cdo1 may be a co-activator of Pparγ in adipogenesis, and may contribute to the development of disease associated with excessive adipose tissue.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Peng
authors: P
authors: Deng
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Yi
authors: Y
authors: Chen
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Ning
authors: N
authors: Ji
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Yunfeng
authors: Y
authors: Lin
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Quan
authors: Q
authors: Yuan
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China.
authors: Ling
authors: L
authors: Ye
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, SiChuan University, Chengdu, Sichuan Province, China. Electronic address: qmchen@scu.edu.cn.
authors: Qianming
authors: Q
authors: Chen
mesh_terms: D041721:3T3-L1 Cells / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D050558:Cysteine Dioxygenase / Q000235:genetics / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D066292:Lipid Droplets / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015854:Up-Regulation
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D050558:Cysteine Dioxygenase
keywords: Adipogenesis; Cdo1; MSCs; Pparγ; Pre-adipocytes