title: New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells.
abstract: A new linear pyranocoumarin named (-)-hydroxydecursinol (1) and a new biscoumarin named (±)-dahuribiscoumarin (2), together with six known compounds isoimperatorin (3), imperatorin (4), phellopterin (5), isodemethylfuropinarine (6), demethylfuropinarine (7), and (+)-decursinol (8) were isolated from the 75% ethanolic extract of the roots of Angelica dahurica var. formosana cv. Chuanbaizhi. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry, and the structure of 2 was confirmed by single-crystal X-ray diffraction. All of the isolated compounds were evaluated for the inhibition against nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cell line, and exhibited the inhibitory activity on NO production in a concentration-dependent manner. Furthermore, real-time PCR analysis revealed that compounds 2, 5-8 could significantly suppress the expression levels of inducible nitric oxide synthase mRNA in a concentration-dependent manner. And their primary structure-activity relationships of NO inhibitory effects were also briefly discussed. These compounds are potential candidates for further bioassay studies to determine their suitability as drug leads.
authors: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, PR China. Electronic address: 378525114@qq.com.
authors: Gai-Gai
authors: GG
authors: Deng
authors: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, PR China.
authors: Wei
authors: W
authors: Wei
authors: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, PR China. Electronic address: xwyang@bjmu.edu.cn.
authors: Xiu-Wei
authors: XW
authors: Yang
authors: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, PR China.
authors: You-Bo
authors: YB
authors: Zhang
authors: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, PR China.
authors: Wei
authors: W
authors: Xu
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors: Ning-Bo
authors: NB
authors: Gong
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors: Yang
authors: Y
authors: Lü
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors: Feng-Feng
authors: FF
authors: Wang
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D003374:Coumarins / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D003374:Coumarins; D008070:Lipopolysaccharides; C000597870:dahuribiscoumarin; C000597868:hydroxydecursinol; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
keywords: (+)-Decursinol (PubChem CID: 442127); (±)-Dahuribiscoumarin; (−)-Hydroxydecursinol; Angelica dahurica var. formosana cv. Chuanbaizhi; Coumarins; Imperatorin (PubChem CID: 10212); Inducible nitric oxide synthase; Isoimperatorin (PubChem CID: 68081); Nitric oxide; Phellopterin (PubChem CID: 98608)