title: Research on the bioactivity of isoquercetin extracted from marestail on bladder cancer EJ cell and the mechanism of its occurrence.
abstract: Research studies in recent years have found that isoquercetin has an inhibiting effect on multiple carcinogens, but research studies filed on isoquercetin in bladder cancer are quite few. This paper observed the influence of isoquercetin on biological activity of the EJ cell of bladder cancer through HC dyeing and trypan blue counting, studied the EJ cell cycle by flow cytometry (FCM), and then analyzed the influence of isoquercetin and its effect on the protein expression of STAT3 and STAT3-inhibiting factors (PIAS3) in EJ cells. Research has shown that isoquercetin has an inhibitory effect on the EJ cells of bladder cancer, but it is not obvious.
authors: a Department of Anesthesiology , The First Affiliated Hospital of Zhengzhou University , No.1 East-JianShe Road, Zhengzhou, Henan , China.
authors: Juhong
authors: J
authors: Ran
authors: a Department of Anesthesiology , The First Affiliated Hospital of Zhengzhou University , No.1 East-JianShe Road, Zhengzhou, Henan , China.
authors: Yanping
authors: Y
authors: Wang
authors: a Department of Anesthesiology , The First Affiliated Hospital of Zhengzhou University , No.1 East-JianShe Road, Zhengzhou, Henan , China.
authors: Wei
authors: W
authors: Zhang
authors: a Department of Anesthesiology , The First Affiliated Hospital of Zhengzhou University , No.1 East-JianShe Road, Zhengzhou, Henan , China.
authors: Minyu
authors: M
authors: Ma
authors: b Yantai Hill Hospital of Yantai City , Shandong , China.
authors: Haiying
authors: H
authors: Zhang
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018832:Molecular Chaperones / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010946:Plants, Medicinal / Q000737:chemistry*; D050828:Protein Inhibitors of Activated STAT / Q000096:biosynthesis; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000096:biosynthesis; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; C016527:isoquercitrin; D011794:Quercetin
keywords: EJ cell; STAT3 apoptosis; bladder cancer; isoquercetin