title: Isolation and manipulation of mouse trophoblast stem cells.
abstract: The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre-implantation blastocysts or from the extraembryonic ectoderm of early post-implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage. This unit describes how to derive, maintain, and manipulate TS cells, including DNA transfection and chimera formation.
authors: Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Koji
authors: K
authors: Hayakawa
authors: Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Emi
authors: E
authors: Himeno
authors: Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Tanaka
authors: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom.
authors: Tilo
authors: T
authors: Kunath
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002678:Chimera; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D018274:Electroporation; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005615:Freezing; D008055:Lipids; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014162:Transfection; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned; D008055:Lipids; C086724:Lipofectamine; D016685:Mitomycin; D004247:DNA
keywords: FGF4; TS cells; extraembryonic ectoderm; trophectoderm; trophoblast; trophoblast stem cells
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