title: Frequency of virulence factors in Helicobacter pylori-infected patients with gastritis.
abstract: The outcome of Helicobacter pylori infection has been related to specific virulence-associated bacterial genotypes. The vacuolating cytotoxin (vacA), cagA gene, oipA and babA2 gene are important virulence factor involving gastric diseases. The objective of this study was to assess the relationship between virulence factors of H. pylori and histopathological findings.
  
  
  METHODS
  Gastroduodenoscopy was performed in 436 dyspeptic patients. Antrum biopsy was obtained for detection of H. pylori, virulence factors and for histopathological assessment. The polymerase chain reaction was used to detect virulence factors of H. pylori using specific primers.
  
  
  RESULTS
  vacA genotypes in patients infected with H. pylori were associated with cagA, iceA1 and iceA2. In the patients with H. pylori infection there was a significant relationship between cagA positivity and neutrophil activity (P = 0.004) and chronic inflammation (P = 0.013) and with H. pylori density (P = 0.034). Neutrophil infiltration was found to be more severe in the s1 group than in the s2 group (P = 0.042). Also was a significant relationship between oipA positivity and neutrophil activity (P = 0.004) and with H. pylori density (P = 0.018). No significant relationships were observed between other vacA genotypes and histopathological parameters.
  
  
  CONCLUSIONS
  H. pylori strains showing cagA, vacA s1 and oipA positivity are associated with more severe gastritis in some histological features but virulence factors of H. pylori do not appear to determine the overall pattern of gastritis.
authors: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Loghman
authors: L
authors: Salimzadeh
authors: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Nader
authors: N
authors: Bagheri
authors: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Behnam
authors: B
authors: Zamanzad
authors: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Fatemeh
authors: F
authors: Azadegan-Dehkordi
authors: Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Ghorbanali
authors: G
authors: Rahimian
authors: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Morteza
authors: M
authors: Hashemzadeh-Chaleshtori
authors: Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: Mahmoud
authors: M
authors: Rafieian-Kopaei
authors: Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Mohammad Hossein
authors: MH
authors: Sanei
authors: Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. Electronic address: shirzadeh@SKUMS.ac.ir.
authors: Hedayatollah
authors: H
authors: Shirzad
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D016145:Endoscopy, Digestive System; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012720:Severity of Illness Index; D013223:Statistics as Topic; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Gastritis; Helicobacter pylori; PCR; Virulence factors