title: Processing by convertases is required for glypican-3-induced inhibition of Hedgehog signaling.
abstract: Glypican-3 (GPC3) is one of the six members of the mammalian glypican family. We have previously reported that GPC3 inhibits Hedgehog (Hh) signaling by competing with Patched (Ptc) for Hh binding. We also showed that GPC3 binds with high affinity to Hh through its core protein, but that it does not interact with Ptc. Several members of the glypican family, including GPC3, are subjected to an endoproteolytic cleavage by the furin-like convertase family of endoproteases. Surprisingly, however, we have found that a mutant GPC3 that cannot be processed by convertases is as potent as wild-type GPC3 in stimulating Wnt activity in hepatocellular carcinoma cell lines and 293T cells and in promoting hepatocellular carcinoma growth. In this study, we show that processing by convertases is essential for GPC3-induced inhibition of Hh signaling. Moreover, we show that a convertase-resistant GPC3 stimulates Hh signaling by increasing the binding of this growth factor to Ptc. Consistent with this, we show that the convertase-resistant mutant binds to both Hh and Ptc through its heparan sulfate (HS) chains. Unexpectedly, we found that the mutant core protein does not bind to Hh. We also report that the convertase-resistant mutant GPC3 carries HS chains with a significantly higher degree of sulfation than those of wild-type GPC3. We propose that the structural changes generated by the lack of cleavage determine a change in the sulfation of the HS chains and that these hypersulfated chains mediate the interaction of the mutant GPC3 with Ptc.
authors: From the Biological Sciences, Sunnybrook Research Institute, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M4N 3M5, Canada and.
authors: Mariana
authors: M
authors: Capurro
authors: From the Biological Sciences, Sunnybrook Research Institute, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M4N 3M5, Canada and.
authors: Wen
authors: W
authors: Shi
authors: From the Biological Sciences, Sunnybrook Research Institute, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M4N 3M5, Canada and.
authors: Tomomi
authors: T
authors: Izumikawa
authors: the Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan.
authors: Hiroshi
authors: H
authors: Kitagawa
authors: From the Biological Sciences, Sunnybrook Research Institute, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M4N 3M5, Canada and jorge.filmus@sri.utoronto.ca.
authors: Jorge
authors: J
authors: Filmus
mesh_terms: D016475:3T3 Cells; D000818:Animals; D053673:Glypicans / Q000502:physiology*; D057809:HEK293 Cells; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D043484:Proprotein Convertases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D053673:Glypicans; D053823:Hedgehog Proteins; D043484:Proprotein Convertases
keywords: Convertase; Glycosaminoglycan; Glypican-3; Hedgehog Signaling Pathway; Heparan Sulfate; Proteoglycan
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