title: Identification and analysis of metabolite production with biotechnological potential in Xanthophyllomyces dendrorhous isolates.
abstract: Antarctic microorganisms have developed different strategies to live in their environments, including modifications to their membrane components to regulate fluidity and the production of photoprotective metabolites such as carotenoids. Three yeast colonies (ANCH01, ANCH06 and ANCH08) were isolated from soil samples collected at King George Island, which according to their rDNA sequence analyses, were determined to be Xanthophyllomyces dendrorhous. This yeast is of biotechnological interest, because it can synthesize astaxanthin as its main carotenoid, which is a powerful antioxidant pigment used in aquaculture. Then, the aim of this work was to characterize the ANCH isolates at their molecular and phenotypic level. The isolates did not display any differences in their rDNA and COX1 gene nucleotide sequences. However, ANCH01 produces approximately sixfold more astaxanthin than other wild type strains. Moreover, even though ANCH06 and ANCH08 produce astaxanthin, their main carotenoid was β-carotene. In contrast to other X. dendrorhous strains, the ANCH isolates did not produce mycosporines. Finally, the ANCH isolates had a higher proportion of polyunsaturated fatty acids than other wild type strains. In conclusion, the reported X. dendrorhous isolates are phenotypically different from other wild type strains, including characteristics that could make them more resistant and better able to inhabit their original habitat, which may also have biotechnological potential.
authors: Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Ñuñoa, Santiago, Chile.
authors: Gabriela
authors: G
authors: Contreras
authors: Salvador
authors: S
authors: Barahona
authors: Dionisia
authors: D
authors: Sepúlveda
authors: Marcelo
authors: M
authors: Baeza
authors: Víctor
authors: V
authors: Cifuentes
authors: Jennifer
authors: J
authors: Alcaíno
mesh_terms: D000864:Antarctic Regions; D001487:Basidiomycota / Q000302:isolation & purification* / Q000378:metabolism*; D002338:Carotenoids / Q000032:analysis; D016000:Cluster Analysis; D003511:Cyclohexanols / Q000032:analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D003511:Cyclohexanols; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005231:Fatty Acids, Unsaturated; D002338:Carotenoids; D003576:Electron Transport Complex IV
references: BMC Microbiol. 2012 Nov 06;12:251
references: 23131126
references: Iran J Microbiol. 2011 Dec;3(4):216-21
references: 22530091
references: Appl Microbiol Biotechnol. 2004 Feb;63(6):635-46
references: 14586578
references: Biomed Pharmacother. 2002 Oct;56(8):365-79
references: 12442909
references: Biosci Biotechnol Biochem. 1995 Mar;59(3):482-6
references: 7766188
references: J Basic Microbiol. 2009 Apr;49(2):135-41
references: 18792046
references: J Biol Chem. 1978 Sep 10;253(17):6218-25
references: 355252
references: Iran J Microbiol. 2013 Sep;5(3):293-8
references: 24475339
references: J Clin Microbiol. 2002 May;40(5):1826-30
references: 11980969
references: Int J Food Microbiol. 2012 Apr 16;155(3):191-8
references: 22405355
references: Syst Appl Microbiol. 2005 Oct;28(8):749-54
references: 16261865
references: Folia Microbiol (Praha). 2001;46(5):402-6
references: 11899472
references: BMC Microbiol. 2012 Oct 18;12:235
references: 23075035
references: FEMS Microbiol Lett. 2007 Apr;269(1):1-10
references: 17286572
references: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references: 13671378
references: Yeast. 1998 Aug;14(11):1007-16
references: 9730280
references: Proc Biol Sci. 2003 Aug 7;270 Suppl 1:S96-9
references: 12952648
references: Photochem Photobiol Sci. 2004 Mar;3(3):281-6
references: 14993945
references: J Microbiol Methods. 2002 Jan;48(1):87-93
references: 11733084
references: Trends Microbiol. 2011 Nov;19(11):540-8
references: 21893414
references: FEMS Yeast Res. 2011 Feb;11(1):52-9
references: 20955516
references: Int J Syst Evol Microbiol. 2002 May;52(Pt 3):1033-1038
references: 12054220
references: J Chem Technol Biotechnol. 1992;53(4):313-27
references: 1368195
references: FEMS Yeast Res. 2002 Dec;2(4):495-517
references: 12702266
references: J Basic Microbiol. 2013 Sep;53(9):766-72
references: 23417292
references: Crit Rev Food Sci Nutr. 2006;46(2):185-96
references: 16431409
references: J Biosci Bioeng. 2006 Jan;101(1):38-41
references: 16503289
references: Appl Microbiol Biotechnol. 2011 Feb;89(3):555-71
references: 21046372
references: Antonie Van Leeuwenhoek. 1998 Feb;73(2):163-8
references: 9717574
references: Curr Genet. 1990 Oct;18(3):277-80
references: 2249259
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353
references: Trends Genet. 1995 Jan;11(1):8
references: 7900196
references: J Ind Microbiol Biotechnol. 2008 Mar;35(3):151-8
references: 18000694
references: Antimicrob Agents Chemother. 1998 Jul;42(7):1756-61
references: 9661017
references: Appl Environ Microbiol. 2007 Feb;73(4):1120-5
references: 17189439
references: Appl Environ Microbiol. 1989 Jan;55(1):116-24
references: 16347815
references: Mycol Res. 2008 Jul;112(Pt 7):861-7
references: 18501574
references: Chem Rev. 2003 Jun;103(6):2203-37
references: 12797829
references: Int J Food Microbiol. 2003 Feb 25;81(1):63-71
references: 12423919
references: FEMS Microbiol Ecol. 2009 Aug;69(2):143-57
references: 19527292
references: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3901-6
references: 17360450
references: Persoonia. 2008 Dec;21:57-69
references: 20396577
references: FEMS Microbiol Ecol. 2009 Sep;69(3):363-72
references: 19624740
references: Yeast. 1995 Feb;11(2):101-10
references: 7732720
references: EMBO Rep. 2006 Apr;7(4):385-9
references: 16585939
references: Biosci Biotechnol Biochem. 2005 Dec;69(12):2381-8
references: 16377897
references: FEMS Yeast Res. 2007 Dec;7(8):1399-408
references: 17825066
references: Mutat Res. 2000 Jun 30;451(1-2):13-24
references: 10915862
references: Microb Cell Fact. 2010 Sep 23;9:73
references: 20863365
references: Genetics. 2008 Feb;178(2):851-72
references: 18245339
references: FEMS Yeast Res. 2011 Mar;11(2):192-201
references: 21155970