title: Exoproteome analysis ofClostridium cellulovoransin natural soft-biomass degradation.
abstract: Clostridium cellulovoransis an anaerobic, cellulolytic bacterium, capable of effectively degrading various types of soft biomass. Its excellent capacity for degradation results from optimization of the composition of the protein complex (cellulosome) and production of non-cellulosomal proteins according to the type of substrates. In this study, we performed a quantitative proteome analysis to determine changes in the extracellular proteins produced byC. cellulovoransfor degradation of several types of natural soft biomass.C. cellulovoranswas cultured in media containing bagasse, corn germ, rice straw (natural soft biomass), or cellobiose (control). Using an isobaric tag method and a liquid chromatograph equipped with a long monolithic silica capillary column/mass spectrometer, we identified 372 proteins in the culture supernatant. Of these, we focused on 77 saccharification-related proteins of both cellulosomal and non-cellulosomal origins. Statistical analysis showed that 18 of the proteins were specifically produced during degradation of types of natural soft biomass. Interestingly, the protein Clocel_3197 was found and commonly involved in the degradation of every natural soft biomass studied. This protein may perform functions, in addition to its known metabolic functions, that contribute to effective degradation of natural soft biomass.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto Japan.
authors: Kohei
authors: K
authors: Esaka
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto Japan.
authors: Shunsuke
authors: S
authors: Aburaya
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto Japan ; Kyoto Integrated Science and Technology Bio-Analysis Center, Shimogyo-ku, Kyoto Japan.
authors: Hironobu
authors: H
authors: Morisaka
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto Japan.
authors: Kouichi
authors: K
authors: Kuroda
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto Japan ; Kyoto Integrated Science and Technology Bio-Analysis Center, Shimogyo-ku, Kyoto Japan.
authors: Mitsuyoshi
authors: M
authors: Ueda
keywords: Cellulosome; Clostridium cellulovorans; Monolithic column; Proteome analysis; Soft-biomass degradation
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