title: Intestinal anti-inflammatory activity of apigenin K in two rat colitis models induced by trinitrobenzenesulfonic acid and dextran sulphate sodium.
abstract: Flavonoids are polyphenolic compounds that are widespread in nature, and consumed as part of the human diet in significant amounts. The aim of the present study was to test the intestinal anti-inflammatory activity of apigenin K, a soluble form of apigenin, in two models of rat colitis, namely the trinitrobenzenesulfonic acid (TNBS) model and the dextran sulphate sodium (DSS) model. Apigenin K (1, 3 and 10 mg/kg; by the oral route; n 4-6 per group) was administered as a pre-treatment to rats with TNBS and DSS colitis, and colonic status was checked by macroscopic and biochemical examination. Apigenin K pre-treatment resulted in the amelioration of morphological signs and biochemical markers in the TNBS model. The results demonstrated a reduction in the inflamed area, as well as lower values of score and colonic weight:length ratio compared with the TNBS group. Myeloperoxidase (MPO) activity was reduced by 30 % (P< 0·05). Moreover, apigenin K pre-treatment ameliorated morphological signs and biochemical markers in the DSS model. Thus, macroscopic damage was significantly reduced and the colonic weight:length ratio was lowered by approximately 10 %, while colonic MPO and alkaline phosphatase activities were decreased by 35 and 21 %, respectively (P< 0·05). Apigenin K pre-treatment also tended to normalise the expression of a number of colonic inflammatory markers (e.g. TNF-α, transforming growth factor-β, IL-6, intercellular adhesion molecule 1 or chemokine (C-C motif) ligand 2). In conclusion, apigenin K is found to have anti-inflammatory effects in two preclinical models of inflammatory bowel disease.
authors: Department of Pharmacology,CIBERehd, School of Pharmacy, University of Granada,Granada,Spain.
authors: Cristina
authors: C
authors: Mascaraque
authors: Department of Biochemistry and Molecular Biology II,CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.Granada, University of Granada,Granada,Spain.
authors: Raquel
authors: R
authors: González
authors: Department of Biochemistry and Molecular Biology II,CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.Granada, University of Granada,Granada,Spain.
authors: María Dolores
authors: MD
authors: Suárez
authors: Department of Pharmacology,CIBERehd, School of Pharmacy, University of Granada,Granada,Spain.
authors: Antonio
authors: A
authors: Zarzuelo
authors: Department of Pharmacology,CIBERehd, School of Pharmacy, University of Granada,Granada,Spain.
authors: Fermín
authors: F
authors: Sánchez de Medina
authors: Department of Biochemistry and Molecular Biology II,CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.Granada, University of Granada,Granada,Spain.
authors: Olga
authors: O
authors: Martínez-Augustin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D047310:Apigenin / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D003092:Colitis / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005765:Gastrointestinal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009929:Organ Size; D010865:Pilot Projects; D011897:Random Allocation; D017208:Rats, Wistar; D012995:Solubility; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D005765:Gastrointestinal Agents; D018836:Inflammation Mediators; D047310:Apigenin; D014302:Trinitrobenzenesulfonic Acid; D016264:Dextran Sulfate
keywords: Nutraceuticals