title: RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuroglial cell cultures.
abstract: We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
authors: Department of Neuroscience, Imaging and Clinical Sciences, Unit of Functional Biotechnology, Aging Research Center (Ce.S.I.), "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 29, 66100 Chieti, Italy ; Interuniversity Institute of Myology, Italy.
authors: Caterina
authors: 0000-0002-5144-2584
authors: C
authors: Morabito
authors: Interuniversity Institute of Myology, Italy ; Laboratory of Tissue Engineering, Department of Clinical and Experimental Sciences, School of Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.
authors: Nathalie
authors: 0000-0001-7695-0991
authors: N
authors: Steimberg
authors: Interuniversity Institute of Myology, Italy ; Laboratory of Tissue Engineering, Department of Clinical and Experimental Sciences, School of Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.
authors: Giovanna
authors: G
authors: Mazzoleni
authors: Department of Neuroscience, Imaging and Clinical Sciences, Unit of Functional Biotechnology, Aging Research Center (Ce.S.I.), "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 29, 66100 Chieti, Italy ; Interuniversity Institute of Myology, Italy.
authors: Simone
authors: 0000-0002-2916-2965
authors: S
authors: Guarnieri
authors: Department of Neuroscience, Imaging and Clinical Sciences, Unit of Functional Biotechnology, Aging Research Center (Ce.S.I.), "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 29, 66100 Chieti, Italy ; Interuniversity Institute of Myology, Italy.
authors: Giorgio
authors: G
authors: Fanò-Illic
authors: Department of Neuroscience, Imaging and Clinical Sciences, Unit of Functional Biotechnology, Aging Research Center (Ce.S.I.), "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 29, 66100 Chieti, Italy ; Interuniversity Institute of Myology, Italy ; Section of Physiology and Physiopathology, Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy.
authors: Maria A
authors: MA
authors: Mariggiò
mesh_terms: D019149:Bioreactors; D002450:Cell Communication / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology*; D014893:Weightlessness
references: Neurochem Res. 2004 Nov;29(11):2015-35
references: 15662836
references: Biotechnol Bioeng. 2000 Oct 5;70(1):32-40
references: 10940861
references: BMC Physiol. 2001;1:4
references: 11384510
references: Prog Neurobiol. 1996 Jun;49(3):185-214
references: 8878303
references: J Neurosci Res. 2004 Jan 15;75(2):241-52
references: 14705145
references: Protoplasma. 2006 Dec;229(2-4):225-34
references: 17180506
references: J Neurosci Methods. 2009 Jun 15;180(2):243-54
references: 19443039
references: Brain Res Brain Res Rev. 2000 Apr;32(1):57-71
references: 10751657
references: Int J Dev Neurosci. 1993 Aug;11(4):485-92
references: 8237465
references: Glia. 2008 Dec;56(16):1791-8
references: 18649405
references: Int J Dev Neurosci. 2009 Apr;27(2):135-47
references: 19111604
references: Neuroscience. 1993 May;54(2):477-92
references: 8101641
references: J Neurochem. 2009 Feb;108(3):533-44
references: 19187090
references: J Neurosci Methods. 1996 May;66(1):23-33
references: 8794937
references: Brain Res Brain Res Rev. 1998 Nov;28(1-2):19-24
references: 9795111
references: Int J Dev Neurosci. 2000 Apr-Jun;18(2-3):193-9
references: 10715574
references: Trends Neurosci. 2005 Apr;28(4):188-95
references: 15808353
references: Peptides. 2008 Sep;29(9):1486-97
references: 18573569
references: Aviat Space Environ Med. 1991 Dec;62(12):1159-65
references: 1755797
references: Genes Nutr. 2009 Mar;4(1):13-22
references: 19104883
references: Invest Ophthalmol Vis Sci. 2008 Feb;49(2):800-6
references: 18235030
references: Ann N Y Acad Sci. 2002 Oct;974:504-17
references: 12446344
references: Eur J Histochem. 2002;46(3):209-14
references: 12472115
references: Cold Spring Harb Perspect Biol. 2009 Jul;1(1):a002576
references: 20066080
references: Adv Space Biol Med. 1994;4:85-110
references: 7757255
references: J Cell Biol. 1987 May;104(5):1353-60
references: 3571332
references: Stem Cells. 2006 Sep;24(9):2052-9
references: 16728560
references: J Neurosci. 2007 Dec 19;27(51):13949-57
references: 18094232
references: Nat Med. 1998 Aug;4(8):901-7
references: 9701241
references: Prog Neurobiol. 2009 Aug;88(4):221-45
references: 19552996
references: Am J Physiol Renal Physiol. 2001 Jul;281(1):F12-25
references: 11399642
references: J Neurosci. 2001 Aug 15;21(16):6147-58
references: 11487638
references: In Vitro Cell Dev Biol Anim. 2004 May-Jun;40(5-6):159-65
references: 15479120
references: PLoS One. 2013;8(9):e73857
references: 24066080
references: Trends Biotechnol. 2013 Feb;31(2):108-15
references: 23336996
references: Adv Space Biol Med. 2002;8:177-95
references: 12951697
references: Eur J Cancer. 1995;31A(4):453-8
references: 7576944
references: Int Rev Cytol. 2005;245:245-325
references: 16125549
references: Biochim Biophys Acta. 2009 Jun;1793(6):1008-22
references: 19110011
references: Cell Mol Neurobiol. 1997 Oct;17(5):509-19
references: 9353592
references: Neurotoxicology. 2009 Jan;30(1):127-35
references: 19056420
references: Biochim Biophys Acta. 2004 Mar 23;1662(1-2):149-58
references: 15033585