title: Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
abstract: Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT, while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.
authors: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC San Luis Potosí, México.
authors: María A
authors: MA
authors: Ortega-Amaro
authors: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC San Luis Potosí, México.
authors: Aída A
authors: AA
authors: Rodríguez-Hernández
authors: Facultad de Ciencias, Universidad Autónoma de San Luis Potosí San Luis Potosi, Mexico.
authors: Margarita
authors: M
authors: Rodríguez-Kessler
authors: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC San Luis Potosí, México.
authors: Eloísa
authors: E
authors: Hernández-Lucero
authors: Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí San Luis Potosi, Mexico.
authors: Sergio
authors: S
authors: Rosales-Mendoza
authors: Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí San Luis Potosi, Mexico.
authors: Alejandro
authors: A
authors: Ibáñez-Salazar
authors: Facultad de Agronomía, Universidad Autónoma de San Luis Potosí San Luis Potosi, México.
authors: Pablo
authors: P
authors: Delgado-Sánchez
authors: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC San Luis Potosí, México.
authors: Juan F
authors: JF
authors: Jiménez-Bremont
keywords: Arabidopsis thaliana; Lactuca sativa; development; glycine-rich domain protein; indole-3-acetic acid; salt stress
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