title: Basic biological characterization of feline morbillivirus.
abstract: Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.
authors: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Rie
authors: R
authors: Koide
authors: Shoichi
authors: S
authors: Sakaguchi
authors: Takayuki
authors: T
authors: Miyazawa
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006358:Hot Temperature; D018103:Morbillivirus / Q000145:classification* / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication
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