title: Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f.sp. heterophylla mediated by its root exudates in a consecutive monoculture system.
abstract: In this study, quantitative real-time PCR (qPCR) was used to determine the amount of Fusarium oxysporum, an important replant disease pathogen in Pseudostellaria heterophylla rhizospheric soil. Moreover, HPLC was used to identify phenolic acids in root exudates then it was further to explore the effects of the phenolic acid allelochemicals on the growth of F. oxysporum f.sp. heterophylla. The amount of F. oxysporum increased significantly in P. heterophylla rhizosphere soil under a consecutive replant system as monitored through qPCR analysis. Furthermore, the growth of F. oxysporum f.sp. heterophylla mycelium was enhanced by root exudates with a maximum increase of 23.8%. In addition, the number of spores increased to a maximum of 12.5-fold. Some phenolic acids promoted the growth of F. oxysporum f.sp. heterophylla mycelium and spore production. Our study revealed that phenolic acids in the root secretion of P. heterophylla increased long with its development, which was closely related to changes in rhizospheric microorganisms. The population of pathogenic microorganisms such as F. oxysporum in the rhizosphere soil of P. heterophylla also sharply increased. Our results on plant-microbe communication will help to better clarify the cause of problems associated with P. heterophylla under consecutive monoculture treatment.
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Yongpo
authors: Y
authors: Zhao
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Linkun
authors: L
authors: Wu
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Leixia
authors: L
authors: Chu
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Yanqiu
authors: Y
authors: Yang
authors: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China.
authors: Zhenfang
authors: Z
authors: Li
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Saadia
authors: S
authors: Azeem
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Zhixing
authors: Z
authors: Zhang
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Changxun
authors: C
authors: Fang
authors: 1] Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (Fujian Agriculture and Forestry University), Fuzhou 35002, China [2] College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China.
authors: Wenxiong
authors: W
authors: Lin
mesh_terms: D029748:Caryophyllaceae / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D005670:Fusarium / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D062385:Hydroxybenzoates / Q000032:analysis; D053147:Plant Exudates / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012331:RNA, Fungal / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D058441:Rhizosphere; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D062385:Hydroxybenzoates; D053147:Plant Exudates; D012331:RNA, Fungal; C017616:phenolic acid
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