title: Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury.
abstract: Hyperhomocysteinemia is a risk factor for cardiovascular disease and the mechanism of homocysteine (HCY)-induced vascular endothelial cell injury has been intensively studied for many years. Recently, a large number of studies have shown inhibitory effects of genistein (GEN), a soy isoflavone, in the process of endothelial cell injury. In the present study, the protective effects of GEN in HCY-induced endothelial cell inflammatory injury were investigated. A model of HCY-induced endothelial cell (ECV-304) inflammatory injury was established in vitro, and the protective effect of GEN in this procession was explored. According to our results, GEN protected HCY-induced endothelial cell from viability decreases, meanwhile prevented the changes of cell morphology and the production of reactive oxygen species (ROS). The expression of NF-kB P-65, IL-6, and ICAM-1 was all down-regulated. During the HCY-induced endothelial cell injury, the endothelial cell apoptosis and proliferation disorder were alleviated. Therefore, we conclude that HCY-induced endothelial cell inflammatory injury could be blocked by GEN. The present findings suggest that GEN protects HCY-induced endothelial cell inflammatory injury may through reducing the release of ROS, inhibiting NF-kB activation, down-regulating the expression of cytokine IL-6 and adhesion molecules ICAM-1, avoiding inflammatory cells and platelet adhesion, accordingly, leading to a balance of endothelial cell proliferation and apoptosis.
authors: Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, Zhengzhou, 450007, China, hshb_1980@sohu.com.
authors: Shengbo
authors: S
authors: Han
authors: Hui
authors: H
authors: Wu
authors: Wenxue
authors: W
authors: Li
authors: Pan
authors: P
authors: Gao
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006710:Homocysteine / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D020011:Protective Agents; D017382:Reactive Oxygen Species; D051996:Transcription Factor RelA; D006710:Homocysteine; D018799:Intercellular Adhesion Molecule-1; D019833:Genistein
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