title: Cellulosic ethanol production by natural bacterial consortia is enhanced by Pseudoxanthomonas taiwanensis.
abstract: BACKGROUND
  Natural bacterial consortia are considered a promising solution for one-step production of ethanol from lignocellulose because of their adaptation to a wide range of natural lignocellulosic substrates and their capacity for efficient cellulose degradation. However, their low ethanol conversion efficiency has greatly limited the development and application of natural bacterial consortia.
  
  
  RESULTS
  In the present study, we analyzed 16 different natural bacterial consortia from a variety of habitats in China and found that the HP consortium exhibited relatively high ethanol production (2.06 g/L ethanol titer from 7 g/L α-cellulose at 55°C in 6 days). Further studies showed that Pseudoxanthomonas taiwanensis played an important role in the high ethanol productivity of HP and that this strain effectively boosted the ethanol production of various other natural bacterial consortia. Finally, we developed a new consortium, termed HPP, by optimizing the proportion of P. taiwanensis in the HP consortium to achieve the highest ethanol production reported for natural consortia. The ethanol conversion ratio reached 78%, with ethanol titers up to 2.5 g/L.
  
  
  CONCLUSIONS
  In the present study, we found a natural bacterial consortium with outstanding ethanol production performance, and revealed an efficient method with potentially broad applicability for further improving the ethanol production of natural bacterial consortia.
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.|Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
authors: Ran
authors: R
authors: Du
authors: The Tsinghua University-Peking University Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084 China.
authors: Jianbin
authors: J
authors: Yan
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.|Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
authors: Shizhong
authors: S
authors: Li
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.|Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
authors: Lei
authors: L
authors: Zhang
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.|Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
authors: Sandra
authors: S
authors: Zhang
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.|Beijing Engineering Research Center for Biofuels, Tsinghua University, Beijing, 100084 China.
authors: Jihong
authors: J
authors: Li
authors: Institute of New Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084 China.
authors: Gang
authors: G
authors: Zhao
authors: Research Institute of Petroleum Processing, Beijing, 100000 China.
authors: Panlu
authors: P
authors: Qi
keywords: Biomass; Cellulose; Ethanol; Natural consortium; Pseudoxanthomonas taiwanensis
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