title: Nd(III)-induced rice mitochondrial dysfunction investigated by spectroscopic and microscopic methods.
abstract: The production capacity and yield of neodymium (Nd) in China have ranked the first in the world. Because of its unique biophysical and biochemical properties, Nd compounds have entered into the agricultural environment greatly to promote plant growth. Mitochondria play a crucial role in respiration and metabolism during the growth of plants. However, little is known about the mechanism by which Nd act at the mitochondrial level in plant cells. In this study, rice mitochondrial swelling, collapsed transmembrane potential and decreased membrane fluidity were examined to be important factors for mitochondria permeability transition pore (mPTP) opening induced by Nd(III). The protection of cyclosporin A (CsA) and dithiothreitol (DTT) could confirm that Nd(III) could trigger mPTP opening. Additionally, mitochondrial membrane breakdown observed by TEM and the release of cytochrome c (Cyt c) could also elucidate the mPTP opening from another point of view. At last, the study showed that Nd(III) could restrain the mitochondrial membrane lipid peroxide, so it might interact with anionic lipid too. This detection will be conductive to the safe application of Nd compounds in agriculture and food industry.
authors: School of Chemistry and Materials Science, Hubei Engineering University, Xiaogan, 432000, People's Republic of China.
authors: Cai-Fen
authors: CF
authors: Xia
authors: Long
authors: L
authors: Lv
authors: Xin-You
authors: XY
authors: Chen
authors: Bo-Qiao
authors: BQ
authors: Fu
authors: Ke-Lin
authors: KL
authors: Lei
authors: Cai-Qin
authors: CQ
authors: Qin
authors: Yi
authors: Y
authors: Liu
mesh_terms: D045304:Cytochromes c / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008560:Membrane Fluidity / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008853:Microscopy; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D008933:Mitochondrial Swelling / Q000187:drug effects; D009354:Neodymium / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D013057:Spectrum Analysis
chemical_list: D009354:Neodymium; D045304:Cytochromes c
references: J Inorg Biochem. 2002 May 21;90(1-2):43-50
references: 12009254
references: Biochim Biophys Acta. 1996 Jul 18;1275(1-2):5-9
references: 8688451
references: FEBS Lett. 1995 May 22;365(1):7-9
references: 7774718
references: Langmuir. 2005 Jul 19;21(15):6987-94
references: 16008413
references: J Membr Biol. 2011 Dec;244(3):105-12
references: 22045332
references: Biochemistry. 1999 Jul 20;38(29):9295-300
references: 10413503
references: Nanomedicine. 2006 Dec;2(4):217-21
references: 17292146
references: J Biol Chem. 1992 Dec 25;267(36):25648-51
references: 1361185
references: Biophys J. 2008 Sep;95(5):2583-98
references: 18424491
references: Cell Mol Life Sci. 2012 Jun;69(11):1787-97
references: 22179840
references: Mol Pharmacol. 1998 Oct;54(4):722-32
references: 9765516
references: Biochim Biophys Acta. 2005 Jan 7;1706(1-2):40-52
references: 15620364
references: Biochem Biophys Res Commun. 2005 Dec 9;338(1):668-76
references: 16126168
references: Biochim Biophys Acta. 1995 Jul 17;1241(2):139-76
references: 7640294
references: J Biol Chem. 2002 Jan 18;277(3):1780-7
references: 11704674
references: Mitochondrion. 2003 Mar;2(4):237-44
references: 16120324
references: J Biol Chem. 1999 Sep 17;274(38):26683-90
references: 10480870
references: Biochim Biophys Acta. 2007 Jan;1767(1):31-44
references: 17123460
references: Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):946-52
references: 18407825
references: Mitochondrion. 2003 Apr;2(5):319-43
references: 16120331
references: Biol Trace Elem Res. 2013 May;152(2):284-91
references: 23417494
references: J Membr Biol. 2013 May;246(5):375-81
references: 23595822
references: Circ Res. 2007 Mar 2;100(4):460-73
references: 17332437
references: Surgery. 1976 Oct;80(4):465-73
references: 135364
references: Chemosphere. 2014 Apr;100:194-9
references: 24321333
references: Biochemistry. 1998 Feb 3;37(5):1394-402
references: 9477968
references: Mitochondrion. 2006 Aug;6(4):176-85
references: 16890028
references: J Membr Biol. 2011 May;241(1):39-49
references: 21461940
references: Biochem Biophys Res Commun. 2005 Nov 11;337(1):320-4
references: 16188235
references: Mitochondrion. 2003 Mar;2(4):305-11
references: 16120330
references: Biochim Biophys Acta. 2006 May-Jun;1763(5-6):430-41
references: 16545471
references: Chem Biol Interact. 2003 Jul 25;146(1):27-37
references: 12902150