title: Association of miR-1266 with recurrence/metastasis potential in estrogen receptor positive breast cancer patients.
abstract: The Homeobox B13 (HOXB13):Interleukin 17 Receptor B (IL17BR) index of estrogen receptor (ER)-positive breast cancer (ER (+) BC) patients may be a potential biomarker of recurrence/ metastasis. However, effects of microRNA (miRNA) binding to the 3' untranslated region (3 ?UTR) of HOXB13 and IL17BR and its function on recurrence/metastasis in ER (+) BC remains elusive. The aims of this study were to determine the expression of miRNAs that bind to 3 ?UTR of HOXB13 and IL17BR in ER (+) BC patients and asess the effects of these miRNAs on recurrence/metastasis. The expression profiles of HOXB13 and IL17BR were evaluated using RT- PCR in tumors and normal tissue samples from 40 ER (+) BC patients. The expression level of 4 miRNAs, which were predicted to bind the 3 ?UTR of HOXB13 and IL17BR using TargetScan, microRNA.org and miRDB online databases, were further evaluated with RT-PCR. Our findings demonstrated that high miR-1266 levels might be significant prognostic factor for recurrence/metastasis occurrence (3.05 fold p=0.004) and tamoxifen response (3.90 fold; p=0.2514) in ER (+) BC cases. Although we suggest that modulation of miR-1266 expression may be an important mechanism underlying the chemoresistance of ER (+) BC, advanced studies and validation are required.
authors: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey E-mail : egeli@uludag.edu.tr.
authors: Elif Demirdogen
authors: ED
authors: Sevinc
authors: Unal
authors: U
authors: Egeli
authors: Gulsah
authors: G
authors: Cecener
authors: Gulcin
authors: G
authors: Tezcan
authors: Berrin
authors: B
authors: Tunca
authors: Sehsuvar
authors: S
authors: Gokgoz
authors: Ismet
authors: I
authors: Tasdelen
authors: Sahsine
authors: S
authors: Tolunay
authors: Turkkan
authors: T
authors: Evrensel
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics; D018567:Breast Neoplasms, Male / Q000188:drug therapy / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000235:genetics; D053722:Receptors, Interleukin-17; D013629:Tamoxifen / Q000627:therapeutic use
chemical_list: D020413:3' Untranslated Regions; D018931:Antineoplastic Agents, Hormonal; D014408:Biomarkers, Tumor; C484414:HOXB13 protein, human; D018398:Homeodomain Proteins; C408922:IL17RB protein, human; C571915:MIRN1266 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D013629:Tamoxifen