title: A conserved amphipathic helix is required for membrane tubule formation by Yop1p.
abstract: The integral membrane proteins of the DP1 (deleted in polyposis) and reticulon families are responsible for maintaining the high membrane curvature required for both smooth endoplasmic reticulum (ER) tubules and the edges of ER sheets, and mutations in these proteins lead to motor neuron diseases, such as hereditary spastic paraplegia. Reticulon/DP1 proteins contain reticulon homology domains (RHDs) that have unusually long hydrophobic segments and are proposed to adopt intramembrane helical hairpins that stabilize membrane curvature. We have characterized the secondary structure and dynamics of the DP1 family protein produced from the YOP1 gene (Yop1p) and identified a C-terminal conserved amphipathic helix (APH) that, on its own, interacts strongly with negatively charged membranes and is necessary for membrane tubule formation. Analyses of DP1 and reticulon family members indicate that most, if not all, contain C-terminal sequences capable of forming APHs. Together, these results indicate that APHs play a previously unrecognized role in RHD membrane curvature stabilization.
authors: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors: Jacob P
authors: JP
authors: Brady
authors: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors: Jolyon K
authors: JK
authors: Claridge
authors: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors: Peter G
authors: PG
authors: Smith
authors: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom jason.schnell@bioch.ox.ac.uk.
authors: Jason R
authors: JR
authors: Schnell
mesh_terms: D001704:Biopolymers; D002462:Cell Membrane / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary
chemical_list: D001704:Biopolymers; D029968:Escherichia coli Proteins; D008823:Micelles
keywords: DP1; Yop1p; amphipathic helix; reticulon; tubular ER
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