title: Efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model.
abstract: BACKGROUND
  Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.
  
  
  METHODS
  MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.
  
  
  RESULTS
  Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.
  
  
  CONCLUSIONS
  The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.
authors: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Azra
authors: A
authors: Kenarkoohi
authors: Dept. of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Masoud
authors: M
authors: Soleimani
authors: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Taravat
authors: T
authors: Bamdad
authors: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Hoorieh
authors: H
authors: Soleimanjahi
authors: Dept. of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors: Hajar
authors: H
authors: Estiri
authors: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Mohammad Hadi
authors: MH
authors: Razavi-Nikoo
keywords: Adipose Tissue-Derived mouse Mesenchymal Stem Cells; Lentivector; Transduction; cervical cancer
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