title: Detection of vancomycin resistances in enterococci within 3 ½ hours.
abstract: Vancomycin resistant enterococci (VRE) constitute a challenging problem in health care institutions worldwide. Novel methods to rapidly identify resistances are highly required to ensure an early start of tailored therapy and to prevent further spread of the bacteria. Here, a spectroscopy-based rapid test is presented that reveals resistances of enterococci towards vancomycin within 3.5 hours. Without any specific knowledge on the strain, VRE can be recognized with high accuracy in two different enterococci species. By means of dielectrophoresis, bacteria are directly captured from dilute suspensions, making sample preparation very easy. Raman spectroscopic analysis of the trapped bacteria over a time span of two hours in absence and presence of antibiotics reveals characteristic differences in the molecular response of sensitive as well as resistant Enterococcus faecalis and Enterococcus faecium. Furthermore, the spectroscopic fingerprints provide an indication on the mechanisms of induced resistance in VRE.
authors: 1] Leibniz Institute of Photonic Technology (IPHT), Jena, Germany [2] Center for Sepsis Control and Care (CSCC), Jena University Hospital, Germany.
authors: U-Ch
authors: UC
authors: Schröder
authors: Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
authors: C
authors: C
authors: Beleites
authors: Center for Sepsis Control and Care (CSCC), Jena University Hospital, Germany.
authors: C
authors: C
authors: Assmann
authors: 1] Leibniz Institute of Photonic Technology (IPHT), Jena, Germany [2] Center for Sepsis Control and Care (CSCC), Jena University Hospital, Germany.
authors: U
authors: U
authors: Glaser
authors: Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
authors: U
authors: U
authors: Hübner
authors: Institute of Medical Microbiology, Jena University Hospital, Germany.
authors: W
authors: W
authors: Pfister
authors: Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
authors: W
authors: W
authors: Fritzsche
authors: 1] Leibniz Institute of Photonic Technology (IPHT), Jena, Germany [2] Center for Sepsis Control and Care (CSCC), Jena University Hospital, Germany [3] Institute of Physical Chemistry and Abbe Center of Photonics, University Jena, Germany.
authors: J
authors: J
authors: Popp
authors: 1] Leibniz Institute of Photonic Technology (IPHT), Jena, Germany [2] Center for Sepsis Control and Care (CSCC), Jena University Hospital, Germany.
authors: U
authors: U
authors: Neugebauer
mesh_terms: D013293:Enterococcus faecalis / Q000737:chemistry / Q000187:drug effects*; D016984:Enterococcus faecium / Q000737:chemistry / Q000187:drug effects*; D013059:Spectrum Analysis, Raman; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
chemical_list: D014640:Vancomycin
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