title: Genomic and phenotypic characterization of Rhizobium gallicum phage vB_RglS_P106B.
abstract: The phage P106B (vB_RglS_P106B) is a Siphoviridae phage with a narrow spectrum of infectivity, which has been isolated from soils with a history of pea cultivation. The trapping host of P106B is an indigenous strain of Rhizobium gallicum (SO14B-4) isolated from soils associated with Vicia cracca. Phenotypic characterization of the phage revealed that P106B has an approximate burst size of 21 p.f.u. per infected cell with 60 min and 100 min eclipse and latent periods, respectively. Phage P106B was unable to transduce under the conditions tested. The genome of P106B is 56 024 bp in length with a mean DNA G+C content of 47.9 %. The complete genome sequence contains 95 putative ORFs and a single tRNA gene coding for leucine with the anticodon TTA. Putative functions could only be assigned to 22 of the predicted ORFs while a significant number of ORFs (47) shared no sequence similarities to previously characterized proteins. The remaining 26 putative protein-coding genes exhibited a sequence resemblance to other hypothetical proteins. No lysogeny-related genes were found in the P106B genome.
authors: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.
authors: Anupama P
authors: AP
authors: Halmillawewa
authors: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.
authors: Marcela
authors: M
authors: Restrepo-Córdoba
authors: Department of Biology, University of Regina, Regina, SK, Canada.
authors: Christopher K
authors: CK
authors: Yost
authors: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada hynes@ucalgary.ca.
authors: Michael F
authors: MF
authors: Hynes
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016679:Genome, Viral; D023281:Genomics; D008242:Lysogeny; D010641:Phenotype; D012231:Rhizobium / Q000821:virology*; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012988:Soil Microbiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins