title: Kin cell lysis is a danger signal that activates antibacterial pathways of Pseudomonas aeruginosa.
abstract: The perception and response to cellular death is an important aspect of multicellular eukaryotic life. For example, damage-associated molecular patterns activate an inflammatory cascade that leads to removal of cellular debris and promotion of healing. We demonstrate that lysis of Pseudomonas aeruginosa cells triggers a program in the remaining population that confers fitness in interspecies co-culture. We find that this program, termed P. aeruginosa response to antagonism (PARA), involves rapid deployment of antibacterial factors and is mediated by the Gac/Rsm global regulatory pathway. Type VI secretion, and, unexpectedly, conjugative type IV secretion within competing bacteria, induce P. aeruginosa lysis and activate PARA, thus providing a mechanism for the enhanced capacity of P. aeruginosa to target bacteria that elaborate these factors. Our finding that bacteria sense damaged kin and respond via a widely distributed pathway to mount a complex response raises the possibility that danger sensing is an evolutionarily conserved process.
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Michele
authors: M
authors: LeRoux
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Robin L
authors: RL
authors: Kirkpatrick
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Elena I
authors: EI
authors: Montauti
authors: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, United States.
authors: Bao Q
authors: BQ
authors: Tran
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: S Brook
authors: SB
authors: Peterson
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Brittany N
authors: BN
authors: Harding
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: John C
authors: JC
authors: Whitney
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Alistair B
authors: AB
authors: Russell
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Beth
authors: B
authors: Traxler
authors: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, United States.
authors: Young Ah
authors: YA
authors: Goo
authors: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, United States.
authors: David R
authors: DR
authors: Goodlett
authors: Department of Physics, University of Washington, Seattle, United States.
authors: Paul A
authors: PA
authors: Wiggins
authors: Department of Microbiology, University of Washington, Seattle, United States.
authors: Joseph D
authors: JD
authors: Mougous
mesh_terms: D000900:Anti-Bacterial Agents; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*
chemical_list: D000900:Anti-Bacterial Agents
keywords: DAMP; fluorescence microscopy; infectious disease; interbacterial; intercellular signaling; mass spectrometry; microbiology
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