title: Inhibition of thermal induced protein denaturation of extract/fractions of Withania somnifera and isolated withanolides.
abstract: This study describes the in vitro inhibition of protein denaturation of extract/fractions of Withania somnifera and isolated withanolides including 20β hydroxy-1-oxo(22R)-witha-2,5,24 trienolide (1), (20R,22R-14α,20α)-dihydroxy-1-oxowitha-2,5,16,24 tetraenolide (2). The results showed that the extract/fractions of the plant evoked profound inhibitory effect on thermal-induced protein denaturation. The chloroform fraction caused the most dominant attenuation of 68% at 500 μg/mL. The bioactivity-guided isolation from chloroform fraction led to the isolation of compounds 1 and 2 that showed profound protein inhibition with 78.05% and 80.43% effect at 500 μg/mL and thus strongly complimented the activity of extract/fractions. In conclusion, extract/fractions of W. somnifera possessed strong inhibition of protein denaturation that can be attributed to these isolated withanolides.
authors: a Department of Chemistry , Kohat University of Science and Technology , Kohat , Pakistan.
authors: Murad Ali
authors: MA
authors: Khan
authors: b Department of Pharmacy , Abdul Wali Khan University , Mardan 23200 , Pakistan.
authors: Haroon
authors: H
authors: Khan
authors: c Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors: Abdul
authors: A
authors: Rauf
authors: d Materials Chemistry Laboratory, Faculty of Sciences, Mohammed First University , Oujda 60000 , Morocco.
authors: Taibi
authors: T
authors: Ben Hadda
mesh_terms: D006358:Hot Temperature; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D011489:Protein Denaturation; D032341:Withania / Q000737:chemistry*; D054358:Withanolides / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D054358:Withanolides
keywords: Withania somnifera; extract/fractions; inhibition of protein denaturation; withanolides