title: Methods to study primary tumor cells and residual tumor cells in mouse models of oncogene dependence.
abstract: The studies of oncogene dependence are aimed to understand an unfortunate and puzzling aspect of targeted anticancer treatments-their progression to drug resistance. Drug resistance develops from a pool of cells that survive the original treatment, called minimal residual disease. Mouse models based on tetracycline-dependent expression of transgenic oncogenes are used to imitate targeted oncogene blockade and to reproduce minimal residual disease in humans. Here we describe a novel method for generating oncogene-dependent mammary tumors using somatic transfer of transactivator-containing retroviruses into transgenic mice with tetracycline-dependent oncogenes and a method for measuring continuous mitotic activity in epithelial cells in real time.
authors: Institut de Recherches Cliniques de Montréal, Université de Montréal, 110 avenue des Pins Ouest, lab 6125, Montreal, QC, Canada, H2w 1R7.
authors: Caroline
authors: C
authors: Botta
authors: Cedric
authors: C
authors: Darini
authors: Guillaume
authors: G
authors: Darrasse-Jèze
authors: Katrina
authors: K
authors: Podsypanina
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D007267:Injections; D008321:Mammary Glands, Animal / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D018365:Neoplasm, Residual; D009857:Oncogenes; D013194:Staining and Labeling; D013388:Succinimides / Q000378:metabolism; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides