title: RNAPro•SAL: a device for rapid and standardized collection of saliva RNA and proteins.
abstract: The stabilization and processing of salivary transcriptome and proteome biomarkers is a critical challenge due to the ubiquitous nature of nucleases and proteases as well as the inherent instability of these biomarkers. Furthermore, extension of salivary transcriptome and proteome analysis to point-of-care and remote sites requires the availability of self-administered ambient temperature collection and storage tools. To address these challenges, a self-contained whole saliva collection and extraction system, RNAPro•SAL, has been developed that provides rapid ambient temperature collection along with concurrent processing and stabilization of extracellular RNA (exRNA) and proteins. The system was compared to the University of California, Los Angeles (UCLA) standard clinical collection process (standard operating procedure, SOP). Both systems measured total RNA and protein, and exRNA IL-8, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin and ribosomal protein S9 (RPS9) by qPCR. Proteome analysis was measured by EIA analysis of interleukin-8 (IL-8), and β-actin, as well as total protein. Over 97% of viable cells were removed by both methods. The system compared favorably to the labor-intensive clinical SOP, which requires low-temperature collection and isolation, yielding samples with similar protein and exRNA recovery and stability.
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Samantha H
authors: SH
authors: Chiang
authors: Oasis Diagnostics Corporation, Vancouver, WA.
authors: Gerald A
authors: GA
authors: Thomas
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Wei
authors: W
authors: Liao
authors: Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA.
authors: Tristan
authors: T
authors: Grogan
authors: Oasis Diagnostics Corporation, Vancouver, WA.
authors: Robert L
authors: RL
authors: Buck
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Laurel
authors: L
authors: Fuentes
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Maha
authors: M
authors: Yakob
authors: Oasis Diagnostics Corporation, Vancouver, WA.
authors: Mary J
authors: MJ
authors: Laughlin
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Chris
authors: C
authors: Schafer
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Abu
authors: A
authors: Nazmul-Hossain
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: Fang
authors: F
authors: Wei
authors: Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA.
authors: David
authors: D
authors: Elashoff
authors: Oasis Diagnostics Corporation, Vancouver, WA.
authors: Paul D
authors: PD
authors: Slowey
authors: UCLA School of Dentistry, Los Angeles, CA.
authors: David T W
authors: DT
authors: Wong
mesh_terms: D000328:Adult; D002470:Cell Survival; D006801:Humans; D008875:Middle Aged; D055550:Protein Stability; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020871:RNA Stability; D012015:Reference Standards; D015203:Reproducibility of Results; D012463:Saliva / Q000166:cytology / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature; D059467:Transcriptome / Q000235:genetics
chemical_list: D020543:Proteome; D012471:Salivary Proteins and Peptides; D012313:RNA
keywords: ambient temperature; point-of-care settings; saliva diagnostic tool; salivary extracellular RNA; salivary protein
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