title: Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave-assisted fluorescence in situ hybridization.
abstract: Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML-RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd.
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Silvia
authors: http://orcid.org/0000-0001-7822-6550
authors: S
authors: Soriani
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Cinzia
authors: C
authors: Mura
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Anna Rita
authors: AR
authors: Panico
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Anna Maria
authors: AM
authors: Scarpa
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Patrizia
authors: P
authors: Recchimuzzo
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Raffaella
authors: R
authors: Dadati
authors: Department of Hematology, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Renata
authors: R
authors: Farioli
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Gabriella
authors: G
authors: De Canal
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Maria Angela
authors: MA
authors: Mura
authors: Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy.
authors: Clara
authors: C
authors: Cesana
mesh_terms: D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D016130:Immunophenotyping; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D008872:Microwaves; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
keywords: acute promyelocytic leukaemia; fluorescence in situ hybridization; microwave oven