title: Nanoparticle labeling of bone marrow-derived rat mesenchymal stem cells: their use in differentiation and tracking.
abstract: Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies due to their ability to migrate to damaged tissue without inducing immune reaction. Many techniques have been developed to trace MSCs and their differentiation efficacy; however, all of these methods have limitations. Conjugated polymer based water-dispersible nanoparticles (CPN) represent a new class of probes because they offer high brightness, improved photostability, high fluorescent quantum yield, and noncytotoxicity comparing to conventional dyes and quantum dots. We aimed to use this tool for tracing MSCs' fate in vitro and in vivo. MSC marker expression, survival, and differentiation capacity were assessed upon CPN treatment. Our results showed that after CPN labeling, MSC markers did not change and significant number of cells were found to be viable as revealed by MTT. Fluorescent signals were retained for 3 weeks after they were differentiated into osteocytes, adipocytes, and chondrocytes in vitro. We also showed that the labeled MSCs migrated to the site of injury and retained their labels in an in vivo liver regeneration model. The utilization of nanoparticle could be a promising tool for the tracking of MSCs in vivo and in vitro and therefore can be a useful tool to understand differentiation and homing mechanisms of MSCs.
authors: Department of Molecular Biology and Genetics, Bilkent University, 06800 Ankara, Turkey.
authors: Ece
authors: E
authors: Akhan
authors: Department of Chemistry, Bilkent University, 06800 Ankara, Turkey.
authors: Donus
authors: D
authors: Tuncel
authors: Department of Biophysics, Ankara University Faculty of Medicine, 06100 Ankara, Turkey.
authors: Kamil C
authors: KC
authors: Akcali
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D058948:Cell Tracking / Q000379:methods*; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water
references: Nucl Med Biol. 2011 Oct;38(7):961-7
references: 21810549
references: Stem Cells. 2007 Nov;25(11):2896-902
references: 17901396
references: J Nanobiotechnology. 2007 Nov 07;5:9
references: 17988386
references: World J Gastroenterol. 2004 Jan 15;10(2):279-83
references: 14716839
references: Nat Rev Clin Oncol. 2011 Nov;8(11):677-88
references: 21946842
references: Stem Cell Rev. 2010 Jun;6(2):224-33
references: 20180048
references: Nanoscale. 2012 Feb 7;4(3):824-30
references: 22159192
references: Cell Tissue Kinet. 1970 Oct;3(4):393-403
references: 5523063
references: Contrast Media Mol Imaging. 2009 Sep-Oct;4(5):207-19
references: 19810053
references: Methods Mol Biol. 2011;750:79-90
references: 21618084
references: Tissue Eng. 2005 Jul-Aug;11(7-8):1198-211
references: 16144456
references: J Control Release. 2005 Nov 28;108(2-3):472-83
references: 16214254
references: Int J Biomed Imaging. 2012;2012:942326
references: 23197973
references: Stem Cells. 2010 Sep;28(9):1686-702
references: 20629172
references: Biochem Biophys Res Commun. 2010 Apr 2;394(2):405-11
references: 20226164
references: Cell Stem Cell. 2009 Mar 6;4(3):206-16
references: 19265660
references: Magn Reson Imaging. 2010 Sep;28(7):1004-13
references: 20663626
references: Can J Physiol Pharmacol. 2009 Feb;87(2):143-50
references: 19234578
references: Radiology. 2004 Dec;233(3):781-9
references: 15486216
references: Angew Chem Int Ed Engl. 2003 Mar 17;42(11):1210-50
references: 12645054
references: Science. 2006 Apr 14;312(5771):217-24
references: 16614209
references: Cell Death Differ. 2007 Jul;14(7):1336-42
references: 17396130
references: Biomed Res Int. 2014;2014:786234
references: 24949469
references: Eur J Cardiothorac Surg. 1999 Apr;15(4):519-24
references: 10371132
references: Science. 1999 Apr 2;284(5411):143-7
references: 10102814
references: J Cell Mol Med. 2011 Jun;15(6):1266-86
references: 21251219
references: Assay Drug Dev Technol. 2011 Feb;9(1):13-20
references: 21050072
references: Nanoscale. 2010 Apr;2(4):484-94
references: 20644748
references: Artif Organs. 2006 Apr;30(4):213-32
references: 16643380
references: Nat Med. 2003 Sep;9(9):1195-201
references: 12910262
references: Nat Biotechnol. 2007 Feb;25(2):217-9
references: 17259977
references: Stem Cells. 2008 Sep;26(9):2287-99
references: 18566331