title: Rapid and easy multiresidue method for the analysis of antibiotics in meats by ultrahigh-performance liquid chromatography-tandem mass spectrometry.
abstract: This study involved the development of a multiresidue method for the rapid analysis of 43 antibiotics in meats using ultrahigh-performance liquid chromatography-tandem mass spectrometry. This method was performed using dispersive-solid phase extraction, which is able to analyze 20 samples within 2 h. All compounds were determined simultaneously on a C18 separation column with gradient elution. Validation of the analytical method was performed by carrying out linearity, limit of quantification (LOQ), accuracy, precision, and recovery tests in different meat products. The validation criteria were set according to AOAC International and Japanese validation guidelines. The linearity of each compound was almost the coefficient of determination (r(2)) > 0.98. The LOQs of all tested antibiotics were <10 μg/kg. The results verify that this method is capable of quantitative analysis of 36, 33, and 37 compounds in beef, pork, and chicken, respectively. This method can be used for rapid and easy multiresidue screening of antibiotics for three meats (pork, beef, and chicken).
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors: Takahiro
authors: T
authors: Yamaguchi
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors: Masahiro
authors: M
authors: Okihashi
authors: §Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.|#Global Collaboration Center, Osaka University, 2-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Kazuo
authors: K
authors: Harada
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors: Kotaro
authors: K
authors: Uchida
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors: Yoshimasa
authors: Y
authors: Konishi
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
authors: Keiji
authors: K
authors: Kajimura
authors: §Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.|#Global Collaboration Center, Osaka University, 2-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Kazumasa
authors: K
authors: Hirata
authors: †Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.|#Global Collaboration Center, Osaka University, 2-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Yoshimasa
authors: Y
authors: Yamamoto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000737:chemistry* / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D052616:Solid Phase Extraction; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: antibiotic residues; dispersive solid-phase extraction; meat; ultrahigh-performance liquid chromatography−tandem mass spectrometry