title: Near-infrared fluorescence probe for the determination of acid phosphatase and imaging of prostate cancer cells.
abstract: In this paper, we developed a near-infrared mercaptopropionic acid (MPA)-capped CuInS2 quantum dot (QD) fluorescence probe for the detection of acid phosphatases (ACP), which is an important biomarker and indicator of prostate cancer. The fluorescence of CuInS2 QDs could be quenched by Cu(2+), and then the addition of adenosine-5'-triphosphate (ATP) could effectively turn on the quenched fluorescence due to the strong interaction between Cu(2+) and ATP. The ACP could catalyze the hydrolysis of ATP, which would disassemble the complex of Cu(2+)-ATP. Therefore, the recovered fluorescence could be quenched again by the addition of ACP. In our method, the limit of detection (LOD) is considerably low for ACP detection in solution. Using the CuInS2 QDs fluorescence probe, we successfully performed in vitro imaging of human prostate cancer cells.
authors: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China. suxg@jlu.edu.cn.
authors: Zihan
authors: Z
authors: Lin
authors: Ziping
authors: Z
authors: Liu
authors: Hao
authors: H
authors: Zhang
authors: Xingguang
authors: X
authors: Su
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000378:metabolism; D003300:Copper / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007204:Indium / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013440:Sulfides / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D013440:Sulfides; D007204:Indium; D003300:Copper; D000255:Adenosine Triphosphate; D000135:Acid Phosphatase