title: N-linked glycoproteome profiling of seedling leaf in Brachypodium distachyon L.
abstract: Brachypodium distachyon L., a model plant for cereal crops, has become important as an alternative and potential biofuel grass. In plants, N-glycosylation is one of the most common and important protein modifications, playing important roles in signal recognition, increase in protein activity, stability of protein structure, and formation of tissues and organs. In this study, we performed the first glycoproteome analysis in the seedling leaves of B. distachyon. Using lectin affinity chromatography enrichment and mass-spectrometry-based analysis, we identified 47 glycosylation sites representing 46 N-linked glycoproteins. Motif-X analysis showed that two conserved motifs, N-X-T/S (X is any amino acid, except Pro), were significantly enriched. Further functional analysis suggested that some of these identified glycoproteins are involved in signal transduction, protein trafficking, and quality control and the modification and remodeling of cell-wall components such as receptor-like kinases, protein disulfide isomerase, and polygalacturonase. Moreover, transmembrane helices and signal peptide prediction showed that most of these glycoproteins could participate in typical protein secretory pathways in eukaryotes. The results provide a general overview of protein N-glycosylation modifications during the early growth of seedling leaves in B. distachyon and supplement the glycoproteome databases of plants.
authors: †College of Life Science, Capital Normal University, Xisanhuan Beilu No. 105, 100048 Beijing, China.|‡College of Life Science, Heze University, University Road No. 2269, 274015 Shandong, China.
authors: Ming
authors: M
authors: Zhang
authors: †College of Life Science, Capital Normal University, Xisanhuan Beilu No. 105, 100048 Beijing, China.
authors: Guan-Xing
authors: GX
authors: Chen
authors: †College of Life Science, Capital Normal University, Xisanhuan Beilu No. 105, 100048 Beijing, China.
authors: Dong-Wen
authors: DW
authors: Lv
authors: †College of Life Science, Capital Normal University, Xisanhuan Beilu No. 105, 100048 Beijing, China.
authors: Xiao-Hui
authors: XH
authors: Li
authors: †College of Life Science, Capital Normal University, Xisanhuan Beilu No. 105, 100048 Beijing, China.|§Hubei Collaborative Innovation Center for Grain Industry, Jing Secret Road No. 88, 434025 Jingzhou, China.
authors: Yue-Ming
authors: YM
authors: Yan
mesh_terms: D058431:Brachypodium / Q000235:genetics*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D036226:Seedlings / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins
keywords: Brachypodium distachyon; glycoproteome; glycosylation motif; leaf; lectin affinity chromatography enrichment