title: Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage (Brassica campestris L. ssp. Pekinensis) leaves.
abstract: The beneficial role of selenium (Se) in alleviation of chromium (Cr)-induced oxidative stress is well established. However, little is known about the underlying mechanism. The impacts of exogenous Se (0.1mg/L) on Cr(1mg/L)-induced oxidative stress and antioxidant systems in leaves of cabbage (Brassica campestris L. ssp. Pekinensis) were investigated by using cellular and biochemical approaches. The results showed that supplementation of the medium with Se was effective in reducing Cr-induced increased levels of lipid peroxides and superoxide free radicals (O(-)2(·)), as well as increasing activities of superoxide dismutase (SOD) and peroxidase (POD). Meanwhile, 1mg/L Cr induced loss of plasma membrane integrity, growth inhibition, as well as ultrastructural changes of leaves were significantly reversed due to Se supplementation in the medium. In addition, Se application significantly altered the subcellular distribution of Cr which transported from mitochondria, nucleus and the cell-wall material to the soluble fraction and chloroplasts. However, Se application did no significant alteration of Cr effects on osmotic adjustment accumulating products. The study suggested that Se is able to protect leaves of cabbage against Cr toxicity by alleviation of Cr induced oxidative stress, and re-distribution of Cr in the subcellular of the leaf. Furthermore, free radicals, lipid peroxides, activity of SOD and POD, and subcellular distribution of Cr can be considered the efficient biomarkers to indicate the efficiency of Se to detoxification Cr.
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Xuejiao
authors: X
authors: Qing
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Pollution Processes and Environmental Criteria (Nankai University), Ministry of Education, Tianjin 300071, China; Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality, Ministry of Agriculture, Guanghou 510640, China. Electronic address: xhzhao@mail.hzau.edu.cn.
authors: Xiaohu
authors: X
authors: Zhao
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Chengxiao
authors: C
authors: Hu
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Peng
authors: P
authors: Wang
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Ying
authors: Y
authors: Zhang
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Xuan
authors: X
authors: Zhang
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Pengcheng
authors: P
authors: Wang
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Hanzhi
authors: H
authors: Shi
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Fen
authors: F
authors: Jia
authors: Hubei Provincial Engineering Laboratory for New-type Fertilizer, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
authors: Chanjuan
authors: C
authors: Qu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001937:Brassica / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002857:Chromium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D008054:Lipid Peroxides / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D012643:Selenium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004785:Environmental Pollutants; D008054:Lipid Peroxides; D002857:Chromium; D013481:Superoxides; C074702:chromium hexavalent ion; D009195:Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium
keywords: Antioxidant system; Brassica campestris L. ssp. Pekinensis; Chromium; Detoxification; Oxidative stress; Selenium