title: High-throughput screening assay for the environmental water samples using cellular response profiles.
abstract: Chemical and physical analyses are commonly used as screening methods for the environmental water. However, these methods can only look for the targeted substance but may miss unexpected toxicants. Furthermore, the synergistic effects of mixture cannot be detected. In order to set up the assay criteria for determining various biological activities at a cellular level that could potentially lead to toxicity of environmental water samples, a novel test based on cellular response by using Real-Time Cellular Analyzer (RTCA) is proposed in this study. First, the water sample is diluted to a series of strengths (80%, 60%, 40%, 30%, 20% and 10%) to get the multi-concentration cellular response profile. Then, the area under the cellular response profile (AUCRP) is calculated. Comparing to the normal cell growth of negative control, a new biological activity index named Percentage of Effect (PoE) has been presented which reflects the cumulative inhibitory activity of cell growth over the log-phase. Finally, a synthetical index PoE50 is proposed to evaluate the intensity of biological activities in water samples. The biological experiment demonstrates the effectiveness of the proposed method.
authors: School of Electrical & Information Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Department of Chemical & Materials Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2G6. Electronic address: thpan@ujs.edu.cn.
authors: Tianhong
authors: T
authors: Pan
authors: School of Electrical & Information Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: lzming@ujs.edu.cn.
authors: Haoran
authors: H
authors: Li
authors: Department of Chemical & Materials Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2G6; Department of Mathematics, Indian Institute of Technology, Kharagpur, West Bengal 721302, India. Electronic address: srkhare@maths.iitkgp.ernet.in.
authors: Swanand
authors: S
authors: Khare
authors: Department of Chemical & Materials Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2G6. Electronic address: biao.huang@ualberta.ca.
authors: Biao
authors: B
authors: Huang
authors: Alberta Centre for Toxicology, University of Calgary, Calgary, Alberta, Canada T2N 4N1. Electronic address: yhuang@ucalgary.ca.
authors: Dorothy
authors: D
authors: Yu Huang
authors: Alberta Health, Edmonton, Alberta, Canada T5J 1S6. Electronic address: weiping.zhang@gov.ab.ca.
authors: Weiping
authors: W
authors: Zhang
authors: Division of Analytical and Environmental Toxicology, University of Alberta, Edmonton, Alberta, Canada T6G 2G3. Electronic address: sgabos@ualberta.ca.
authors: Stephan
authors: S
authors: Gabos
mesh_terms: D048708:Cell Growth Processes / Q000187:drug effects; D002460:Cell Line; D054750:Ecotoxicology / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical
keywords: Area under the cellular response profile (AUCRP); Biological activity; Concentration–response curve; Cytotoxicity; High-throughput screening (HTS)