title: In vitro anti-hyperglycemic activity of 4-hydroxyisoleucine derivatives.
abstract: The nonproteinogenic amino acid, 4-hydroxyisoleucine (1) has been isolated in large quantities from the fenugreek (T. foenum-graecum) seeds. Few novel derivatives (3-11 and 13-18) were prepared from the naturally occurring 4-hydroxyisoleucine (1) and screened for their in vitro glucose uptake stimulatory effect in L-6 skeletal muscle cells. The derivatives 6, 7, 8, 10 and 11 exhibited better glucose uptake stimulatory activity than parent compound, 4-hydroxyisoleucine at 5 and 10µM concentrations and compounds 7 and 11 enhanced translocation of insulin sensitive glucose transporters-4 in skeletal muscle cells.
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
authors: Venkateswarlu
authors: V
authors: Korthikunta
authors: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
authors: Jyotsana
authors: J
authors: Pandey
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
authors: Rohit
authors: R
authors: Singh
authors: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
authors: Rohit
authors: R
authors: Srivastava
authors: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
authors: Arvind K
authors: AK
authors: Srivastava
authors: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India. Electronic address: akhilesh_tamrakar@cdri.res.in.
authors: Akhilesh K
authors: AK
authors: Tamrakar
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India. Electronic address: t_narendra@cdri.res.in.
authors: Narender
authors: N
authors: Tadigoppula
mesh_terms: D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007532:Isoleucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D032342:Muscle Cells / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology; D051381:Rats; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry
chemical_list: C104672:4-hydroxyisoleucine; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; C495694:Slc2a4 protein, rat; D007532:Isoleucine; C502516:(2S,3S,4R)-gamma-hydroxyisoleucine; D005947:Glucose
keywords: 4-Hydroxyisoleucine; Anti-hyperglycemic activity; Fenugreek seeds; GLUT4 translocation; Glucose uptake