title: Capillary zone electrophoresis on-line coupled to mass spectrometry: A perspective application for clinical proteomics.
abstract: Clinical proteomics, a rapidly growing field, intends to use specific diagnostic proteomic/peptidomic markers for initial diagnosis or prognosis of the progression of various diseases. Analyses of disease-associated markers in defined biological samples can provide valuable molecular diagnostic information for these diseases. This approach relies on sensitive and highly standardized modern analytical techniques. In the recent years, one of these technologies, CZE online coupled to MS (CZE-MS), has been increasingly used for the detection of peptide biomarkers (<20 kDa) in body fluids such as urine. This review presents the most relevant urinary proteomic studies addressing the application of CZE-MS in clinically relevant biomarker research between the years 2006 and 2014.
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Martin
authors: M
authors: Pejchinovski
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Dajana
authors: D
authors: Hrnjez
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Adela
authors: A
authors: Ramirez-Torres
authors: Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
authors: Vasiliki
authors: V
authors: Bitsika
authors: Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
authors: George
authors: G
authors: Mermelekas
authors: Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.|School of Biomedical and Healthcare Sciences, Plymouth University, Plymouth, UK.
authors: Antonia
authors: A
authors: Vlahou
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Petra
authors: P
authors: Zürbig
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.|Institute of Cardiovascular and Medical Sciences, University of Glasgow, UK.
authors: Harald
authors: H
authors: Mischak
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Jochen
authors: J
authors: Metzger
authors: Mosaiques Diagnostics GmbH, Hanover, Germany.
authors: Thomas
authors: T
authors: Koeck
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D002318:Cardiovascular Diseases / Q000652:urine; D019075:Electrophoresis, Capillary; D006086:Graft vs Host Disease / Q000652:urine; D006801:Humans; D009369:Neoplasms / Q000652:urine; D011379:Prognosis; D011507:Proteinuria / Q000652:urine*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Biomarker; Capillary electrophoresis-mass spectrometry; Clinical; Proteomics