title: Suitability of capillary blood for quantitative assessment of G6PD activity and performances of G6PD point-of-care tests.
abstract: The use of primaquine and other 8-aminoquinolines for malaria elimination is hampered by, among other factors, the limited availability of point-of-care tests for the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency. Historically, the most used source of blood for G6PD analyses is venous blood, whereas diagnostic devices used in the field require the use of capillary blood; data have shown that the two sources of blood often differ with respect to hemoglobin concentration and number of red blood cells. Therefore, we have analyzed, in both capillary and venous blood drawn from the same healthy donors, the correlation of G6PD activity assessed by two qualitative tests (the Fluorescent Spot test and the CareStart test) with the gold standard quantitative spectrophotometric assay. Results obtained on 150 subjects with normal, intermediate, and deficient G6PD phenotypes show that, although differences exist between the aforementioned characteristics in capillary and venous blood, these do not impact on the quantitative assessment of G6PD activity after corrected for hemoglobin concentration or red blood cell count. Furthermore, we have assessed the sensitivity and specificity of the two qualitative tests against the gold standard spectrophotometric assay at different activity thresholds of residual enzymatic activity in both blood sources.
authors: Germana
authors: G
authors: Bancone
authors: Cindy S
authors: CS
authors: Chu
authors: Nongnud
authors: N
authors: Chowwiwat
authors: Raweewan
authors: R
authors: Somsakchaicharoen
authors: Pornpimon
authors: P
authors: Wilaisrisak
authors: Prakaykaew
authors: P
authors: Charunwatthana
authors: Pooja
authors: P
authors: Bansil
authors: Sarah
authors: S
authors: McGray
authors: Gonzalo J
authors: GJ
authors: Domingo
authors: François H
authors: FH
authors: Nosten
mesh_terms: D000634:Aminoquinolines / Q000627:therapeutic use; D000962:Antimalarials / Q000627:therapeutic use*; D002196:Capillaries; D004796:Clinical Enzyme Tests / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000235:genetics; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000235:genetics*; D006801:Humans; D016780:Malaria, Vivax / Q000188:drug therapy*; D008297:Male; D019095:Point-of-Care Systems; D011319:Primaquine / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D014680:Veins
chemical_list: D000634:Aminoquinolines; D000962:Antimalarials; D005954:Glucosephosphate Dehydrogenase; D011319:Primaquine; C080436:8-aminoquinoline
references: Int J Lab Hematol. 2011 Oct;33(5):463-70
references: 21501392
references: Malar J. 2010;9:223
references: 20684792
references: Malar J. 2011;10:368
references: 22171972
references: Malar J. 2013;12:210
references: 23782846
references: Malar J. 2013;12:289
references: 23965028
references: PLoS One. 2013;8(8):e72721
references: 23991145
references: Malar J. 2013;12:418
references: 24228846
references: Lancet Infect Dis. 2014 Feb;14(2):130-9
references: 24239324
references: Pediatr Hematol Oncol. 2014 Feb;31(1):95-100
references: 24483842
references: Am J Trop Med Hyg. 2014 Jul;91(1):77-80
references: 24778197
references: Am J Trop Med Hyg. 2014 Oct;91(4):854-61
references: 25071003
references: J Med Screen. 2000;7(1):46-51
references: 10807147
references: Southeast Asian J Trop Med Public Health. 2003 Jun;34(2):405-14
references: 12971572
references: Blood. 1968 Nov;32(5):816-8
references: 4386875
references: Br J Haematol. 1977 Feb;35(2):331-40
references: 857853
references: Scand J Clin Lab Invest. 1988 Nov;48(7):723-6
references: 3201107
references: J Pediatr. 1991 Aug;119(2):293-9
references: 1824550
references: Scand J Clin Lab Invest. 1991 Nov;51(7):651-4
references: 1810025
references: Vox Sang. 2007 Nov;93(4):348-53
references: 18070280
references: J Hum Genet. 2008;53(1):48-54
references: 18046504
references: Am J Trop Med Hyg. 2010 Feb;82(2):210-4
references: 20133993
references: PLoS One. 2011;6(12):e28357
references: 22164279