title: Substrate specificity and biochemical characterization of an adenylation domain from an obligate marine actinomycete.
abstract: Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specifically recognized and activated tryptophan (Trp) and phenylalanine (Phe). The basic kinetic parameters of Sare0357 for Trp were K m = 0.04 mM, V max = 2.1 μM/min, k cat = 14.2 min(-1), and for Phe were K m = 0.03 mM, V max = 1.6 μM/min, kcat = 10.4 min(-1). Our data elucidated Sare0357 biological role and biochemical properties as a Trp and Phe-activating adenylation domain.
authors: Hubei Key Laboratory for Genetic Regulation & Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China, sxxsfh@mails.ccnu.edu.cn.
authors: Xiao
authors: X
authors: Shu
authors: Yanling
authors: Y
authors: Ma
authors: Chong
authors: C
authors: Lu
authors: Ming
authors: M
authors: Lei
authors: Yanli
authors: Y
authors: Liu
authors: Ke
authors: K
authors: Liu
authors: Guomei
authors: G
authors: Xiong
authors: Sisi
authors: S
authors: Xia
authors: Zhijuan
authors: Z
authors: Zhao
authors: Wenkai
authors: W
authors: Luo
authors: Qiao
authors: Q
authors: Fu
authors: Chao
authors: C
authors: Qi
mesh_terms: D059001:Aquatic Organisms / Q000201:enzymology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007700:Kinetics; D008848:Micromonosporaceae / Q000201:enzymology* / Q000302:isolation & purification; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D013379:Substrate Specificity; D014364:Tryptophan / Q000378:metabolism
chemical_list: D010649:Phenylalanine; D014364:Tryptophan; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase