title: MMP-9 expression and activity is concurrent with endothelial cell apoptosis in the basilar artery after subarachnoid hemorrhaging in rats.
abstract: Subarachnoid hemorrhage refers to one kind of lethal stroke. While several studies have shown the role of matrix metalloproteinase 9 in cerebral tissues and microvasculature after subarachnoid hemorrhage the role of matrix metalloproteinase 9 in large cerebral vasculature is limited, The present study aimed at investigating the relationship between the expression and activity of matrix metalloproteinase 9 and vascular endothelial cell apoptosis in the basilar artery at early-stage post-subarachnoid hemorrhaging in rats. The expression and activity of matrix metalloproteinase 9 in the basilar artery increased at 12 h after subarachnoid hemorrhage and peaked at 24 h. Concurrently, the number of apoptotic cells in the basilar artery increased at 12 h and peaked at 24 h following subarachnoid hemorrhage. Additionally, laminin and cleaved caspase 3 reached to their lowest and highest levels at 24 h after subarachnoid hemorrhage, respectively. Our results suggested that the change in expression and activity of matrix metalloproteinase 9 paralleled that of apoptotic cells in the basilar artery following subarachnoid hemorrhage. Matrix metalloproteinase 9 may be contributing to the injury of the basilar artery after subarachnoid hemorrhage via laminin degradation. Although the underlying mechanisms need to be explored further, our study has shown a potential association of matrix metalloproteinase 9 with injury of the basilar artery and can be associated with early-stage brain injury following subarachnoid hemorrhage.
authors: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China, stonegzd@gmail.com.
authors: Zongduo
authors: Z
authors: Guo
authors: Liang
authors: L
authors: Xu
authors: Xiaoying
authors: X
authors: Wang
authors: Xiaochuan
authors: X
authors: Sun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis; D001488:Basilar Artery / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000201:enzymology* / Q000473:pathology*; D005786:Gene Expression Regulation / Q000502:physiology; D020287:In Situ Nick-End Labeling; D007797:Laminin / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic; D013345:Subarachnoid Hemorrhage / Q000473:pathology*; D013997:Time Factors
chemical_list: D007797:Laminin; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9
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