title: Effect of long-term administration of arachidonic acid on n-3 fatty acid deficient mice.
abstract: The effect of long-term oral administration of arachidonic acid (ARA, 240 mg/kg/day) on brain function was assessed for mice maintained on an n-3 fatty acid adequate or deficient diet. The administration of ARA for 13 weeks resulted in an elevation of spontaneous motor activity, or the tendency thereof, in both the n-3 fatty acid adequate and deficient groups. However, the n-3 fatty acid deficient mice that were administered with ARA revealed marked deterioration in motor function in a motor coordination test. In the experiment to investigate changes over time, the motor activity of the ARA-administered group continued to increase mildly in n-3 deficient mice, although that of the control group showed a decrease involving habituation for both diet groups from the second week. The fatty acid composition of the brain at the end of the behavioral experiments indicated an increase in the levels of ARA and other n-6 fatty acids, as well as a decrease in the levels of docosahexaenoic acid. These results suggest that long-term administration of ARA causes an increase of futile spontaneous motor activity and the diminution of motor function by aggravation of n-3 fatty acid deficiency.
authors: Laboratory for Functional Analysis of Marine Materials, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors: Akiko
authors: A
authors: Harauma
authors: Laboratory of Food and Nutritional Science, Department of Food and Life Science, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors: Makiko
authors: M
authors: Tomita
authors: Laboratory of Food and Nutritional Science, Department of Food and Life Science, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors: Daiki
authors: D
authors: Muto
authors: Laboratory for Functional Analysis of Marine Materials, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan; Laboratory of Food and Nutritional Science, Department of Food and Life Science, School of Life and Environmental Science, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: moriguchi@azabu-u.ac.jp.
authors: Toru
authors: T
authors: Moriguchi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage*; D001835:Body Weight / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000378:metabolism; D004334:Drug Administration Schedule; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid
keywords: Arachidonic acid; Brain function; Mice; Motor coordination; Rota-Rod test; Spontaneous motor activity