title: [Effect of Par-4 on the apoptosis of islet β cell].
abstract: OBJECTIVE
  To explore the effect of high glucose and lipid intervention on islet cell apoptosis through the inhibition of prostate apoptosis response factor-4 (Par-4) expression and the underlying mechanisms.
  
  
  METHODS
  The mice islet β cells (NIT-1 cells) were randomly divided into a control group, a Par-4 inhibited group, a glucose and fatty acid intervented group and a glucose and fatty acid intervented+Par-4 inhibited group. Cell apoptosis was detected by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL), and the protein expression levels of Par-4 and glucose regulated protein 78 (GRP78) were detected by Western blot.
  
  
  RESULTS
  Compared with the control group [(3.14 ± 1.08)%], the apoptosis rate of islet beta cell [(33.82 ± 3.15)%] in the glucose and fatty acid intervented group was significantly increased accompanied by the dramatically elevated Par-4 and GRP78 expression (both P<0.05). Compared with the glucose and fatty acid intervented group, the apoptosis rate in glucose and fatty acid intervented+Par-4 inhibit group [(18.3 4 ± 2.11)%] was significantly decreased concomitant with the significantly decreased Par-4 and GRP78 expression (both P<0.05).
  
  
  CONCLUSIONS
  The glucose and fatty acid-induced apoptosis of mice islet β cells could be improved through the inhibition of Par-4 expression, which might be related to reduction of endoplasmic reticulum stress.
authors: Department of Endocrinology, First Affi liated Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Xiaguang
authors: X
authors: Gan
authors: Qi'nan
authors: Q
authors: Wu
authors: Wuquan
authors: W
authors: Deng
authors: Ling
authors: L
authors: Zhang
authors: Bing
authors: B
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002460:Cell Line; D000091342:Endoplasmic Reticulum Chaperone BiP; D005227:Fatty Acids; D005786:Gene Expression Regulation; D005947:Glucose; D006360:Heat-Shock Proteins / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D007515:Islets of Langerhans / Q000166:cytology*; D051379:Mice
chemical_list: D051017:Apoptosis Regulatory Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D005227:Fatty Acids; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; C101296:prostate apoptosis response-4 protein; D005947:Glucose