title: Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori.
abstract: Female moths employ their own pheromone blends as a communicational medium in mating behavior. The biosynthesis and release of sex pheromone in female moths are regulated by pheromone biosynthesis activating neuropeptide (PBAN) and the corresponding action of PBAN has been well elucidated in Bombyx mori. However, very little is known about the molecular mechanism regarding the biosynthesis of sex pheromone precursor. In this study, quantitative proteomics was utilized to comprehensively elucidate the expression dynamics of pheromone glands (PGs) during development. Proteomic analysis revealed a serial of differentially expressed sex pheromone biosynthesis-associated proteins at the different time points of B. mori development. Most interestingly B. mori glycerol-3-phosphate O-acyltransferase (BmGPAT) was found to be expressed during the key periods of sex pheromone biosynthesis. RNAi knockdown of BmGPAT confirmed the important function of this protein in the biosynthesis of sex pheromone precursor, triacylglcerol (TAG), and subsequently PBAN-induced production of sex pheromone, bombykol. Behavioral analysis showed that RNAi knockdown of GPAT significantly impaired the ability of females to attract males. Our findings indicate that GPAT acts to regulate the biosynthesis of sex pheromone precursor, TAG, thus influencing PBAN-induced sex pheromone production and subsequent mating behavior.
authors: State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.
authors: Mengfang
authors: M
authors: Du
authors: State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.
authors: Xiaoguang
authors: X
authors: Liu
authors: State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.
authors: Xiaoming
authors: X
authors: Liu
authors: State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.
authors: Xinming
authors: X
authors: Yin
authors: Translational Research Center, Zhengzhou University People's Hospital, Zhengzhou 450003 P.R. China.
authors: Shuangyin
authors: S
authors: Han
authors: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors: Qisheng
authors: Q
authors: Song
authors: State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.
authors: Shiheng
authors: S
authors: An
mesh_terms: D000595:Amino Acid Sequence; D000825:Animal Structures / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005233:Fatty Alcohols / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000494:pharmacology*; D040901:Proteomics; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D012724:Sex Attractants / Q000096:biosynthesis*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D005233:Fatty Alcohols; D019476:Insect Proteins; D009479:Neuropeptides; D012724:Sex Attractants; C006787:bombykol; D005992:Glycerol-3-Phosphate O-Acyltransferase
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