title: Oil accumulation by the oleaginous diatom Fistulifera solaris as revealed by the genome and transcriptome.
abstract: Oleaginous photosynthetic organisms such as microalgae are promising sources for biofuel production through the generation of carbon-neutral sustainable energy. However, the metabolic mechanisms driving high-rate lipid production in these oleaginous organisms remain unclear, thus impeding efforts to improve productivity through genetic modifications. We analyzed the genome and transcriptome of the oleaginous diatom Fistulifera solaris JPCC DA0580. Next-generation sequencing technology provided evidence of an allodiploid genome structure, suggesting unorthodox molecular evolutionary and genetic regulatory systems for reinforcing metabolic efficiencies. Although major metabolic pathways were shared with nonoleaginous diatoms, transcriptome analysis revealed unique expression patterns, such as concomitant upregulation of fatty acid/triacylglycerol biosynthesis and fatty acid degradation (β-oxidation) in concert with ATP production. This peculiar pattern of gene expression may account for the simultaneous growth and oil accumulation phenotype and may inspire novel biofuel production technology based on this oleaginous microalga.
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan JST, CREST, Chiyoda-ku, Tokyo 102-0075, Japan tsuyo@cc.tuat.ac.jp.
authors: Tsuyoshi
authors: http://orcid.org/0000-0003-2601-2256
authors: T
authors: Tanaka
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors: Yoshiaki
authors: http://orcid.org/0000-0001-9206-2601
authors: Y
authors: Maeda
authors: Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 75005 Paris, France.
authors: Alaguraj
authors: A
authors: Veluchamy
authors: Center for iPS Cell Research and Application, Kyoto University, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo 135-0064, Japan.
authors: Michihiro
authors: M
authors: Tanaka
authors: Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 75005 Paris, France.
authors: Heni
authors: H
authors: Abida
authors: Laboratoire de Physiologie Cellulaire et Végétale, UMR 5168, CNRS-CEA-INRA-Université Grenoble Alpes, CEA Grenoble, 38054, Grenoble Cedex 09, France.
authors: Eric
authors: E
authors: Maréchal
authors: Environmental and Evolutionary Genomics Section, CNRS UMR8197 INSERM U1024, Institut de Biologie de l'Ecole Normale Supérieure, 75005 Paris, France.
authors: Chris
authors: C
authors: Bowler
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan JST, CREST, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Masaki
authors: M
authors: Muto
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan JST, CREST, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Yoshihiko
authors: Y
authors: Sunaga
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan JST, CREST, Chiyoda-ku, Tokyo 102-0075, Japan.
authors: Masayoshi
authors: M
authors: Tanaka
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors: Tomoko
authors: http://orcid.org/0000-0002-4505-2774
authors: T
authors: Yoshino
authors: Mitsubishi Research Institute, Chiyoda-ku, Tokyo 100-8141, Japan.
authors: Takeaki
authors: T
authors: Taniguchi
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors: Yorikane
authors: Y
authors: Fukuda
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors: Michiko
authors: M
authors: Nemoto
authors: JST, CREST, Chiyoda-ku, Tokyo 102-0075, Japan Biotechnology Laboratory, Electric Power Development Co., Wakamatsu-ku, Kitakyusyu, Fukuoka 808-0111, Japan.
authors: Mitsufumi
authors: M
authors: Matsumoto
authors: Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo 135-0064, Japan.
authors: Pui Shan
authors: PS
authors: Wong
authors: Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo 135-0064, Japan.
authors: Sachiyo
authors: S
authors: Aburatani
authors: Center for iPS Cell Research and Application, Kyoto University, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo 135-0064, Japan.
authors: Wataru
authors: W
authors: Fujibuchi
mesh_terms: D017377:Diatoms / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D018745:Genome, Plant / Q000235:genetics; D059467:Transcriptome / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D014280:Triglycerides
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