title: Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.
abstract: Transgenic farm animals are attractive alternative mammalian models to rodents for the study of developmental, genetic, reproductive and disease-related biological questions, as well for the production of recombinant proteins, or the assessment of xenotransplants for human patients. Until recently, the ability to generate transgenic farm animals relied on methods of passive transgenesis. In recent years, significant improvements have been made to introduce and apply active techniques of transgenesis and genetic engineering in these species. These new approaches dramatically enhance the ease and speed with which livestock species can be genetically modified, and allow to performing precise genetic modifications. This paper provides a synopsis of enzyme-mediated genetic engineering in livestock species covering the early attempts employing naturally occurring DNA-modifying proteins to recent approaches working with tailored enzymatic systems.
authors: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina.
authors: Pablo
authors: P
authors: Bosch
authors: Diego O
authors: DO
authors: Forcato
authors: Fabrisio E
authors: FE
authors: Alustiza
authors: Ana P
authors: AP
authors: Alessio
authors: Alejandro E
authors: AE
authors: Fili
authors: María F
authors: MF
authors: Olmos Nicotra
authors: Ana C
authors: AC
authors: Liaudat
authors: Nancy
authors: N
authors: Rodríguez
authors: Thirumala R
authors: TR
authors: Talluri
authors: Wilfried A
authors: WA
authors: Kues
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements / Q000235:genetics*; D003851:Deoxyribonucleases / Q000378:metabolism; D018014:Gene Transfer Techniques / Q000662:veterinary*; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D019426:Integrases / Q000378:metabolism; D058751:Livestock / Q000235:genetics*; D023421:Models, Animal; D008954:Models, Biological; D045522:Recombinases / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D045522:Recombinases; D019426:Integrases; D003851:Deoxyribonucleases
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