title: Radical Scavenging Activities of Tannin Extracted from Amaranth (Amaranthus caudatus L.).
abstract: This study investigates the bioactivity of tannin from amaranth (Amaranthus caudatus L.) extracts. The antioxidant activities of the extracts from amaranth leaves, flowers, and seeds were evaluated. Tannin from leaves of amaranth has been evaluated for superoxide scavenging activity by using DPPH and ABTS(+) analysis, reducing power, protective effect against H2O2-induced oxidative damage in L-132 and BNL-CL2 cells, and inhibition of superoxide radical effects on HL-60 cells. At a concentration of 100 μg/ml, tannin showed protective effects and restored cell survival to 69.2% and 41.8% for L-132 and BNL-CL2 cells, respectively. Furthermore, at the same concentration, tannin inhibited 41% of the activity of the superoxide radical on HL-60 cells and 43.4% of the increase in nitric oxide levels in RAW 264.7 cells. The expression levels of the antioxidant-associated protein SOD-1 were significantly increased in a concentration-dependent manner in RAW 264.7 cells treated with tannin from amaranth leaves. These results suggest that tannin from the leaves of Amaranthus caudatus L. is a promising source of antioxidant component that can be used as a food preservative or nutraceutical.
authors: Department of Food Science and Technology, Seoul National University of Science & Technology, Seoul 139-743, Republic of Korea.
authors: Hyeon-Ju
authors: HJ
authors: Jo
authors: Department of Food Science and Technology, Seoul National University of Science & Technology, Seoul 139-743, Republic of Korea.
authors: Kang-Hyun
authors: KH
authors: Chung
authors: Department of Food & Nutrition, Baewha Women's University, Seoul 110-735, Republic of Korea.
authors: Jin A
authors: JA
authors: Yoon
authors: Department of Advanced Materials Engineering, Daejeon University, Daejeon 300-716, Republic of Korea.
authors: Kwon-Jai
authors: KJ
authors: Lee
authors: Division of Food Bioscience, Konkuk University, Chungju 380-701, Republic of Korea.
authors: Byeong Chun
authors: BC
authors: Song
authors: Division of Food Bioscience, Konkuk University, Chungju 380-701, Republic of Korea.
authors: Jeung Hee
authors: JH
authors: An
mesh_terms: D027721:Amaranthus / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D016877:Oxidants / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013634:Tannins / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D016877:Oxidants; D010936:Plant Extracts; D013634:Tannins