title: Phylogenetic analysis, genomic diversity and classification of M class gene segments of turkey reoviruses.
abstract: From 2011 to 2014, 13 turkey arthritis reoviruses (TARVs) were isolated from cases of swollen hock joints in 2-18-week-old turkeys. In addition, two isolates from similar cases of turkey arthritis were received from another laboratory. Eight turkey enteric reoviruses (TERVs) isolated from fecal samples of turkeys were also used for comparison. The aims of this study were to characterize turkey reovirus (TRV) based on complete M class genome segments and to determine genetic diversity within TARVs in comparison to TERVs and chicken reoviruses (CRVs). Nucleotide (nt) cut off values of 84%, 83% and 85% for the M1, M2 and M3 gene segments were proposed and used for genotype classification, generating 5, 7, and 3 genotypes, respectively. Using these nt cut off values, we propose M class genotype constellations (GCs) for avian reoviruses. Of the seven GCs, GC1 and GC3 were shared between the TARVs and TERVs, indicating possible reassortment between turkey and chicken reoviruses. The TARVs and TERVs were divided into three GCs, and GC2 was unique to TARVs and TERVs. The proposed new GC approach should be useful in identifying reassortant viruses, which may ultimately be used in the design of a universal vaccine against both chicken and turkey reoviruses.
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States.
authors: Sunil K
authors: SK
authors: Mor
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States.
authors: Douglas
authors: D
authors: Marthaler
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States.
authors: Harsha
authors: H
authors: Verma
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States.
authors: Tamer A
authors: TA
authors: Sharafeldin
authors: Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125 004, India.
authors: Naresh
authors: N
authors: Jindal
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States.
authors: Robert E
authors: RE
authors: Porter
authors: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, United States. Electronic address: goyal001@umn.edu.
authors: Sagar M
authors: SM
authors: Goyal
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D030101:Orthoreovirus, Avian / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D014422:Turkeys / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Genotypes; M class gene segments; Mutations; Phylogenetic analysis; Reassortment; Turkey arthritis reovirus