title: Simultaneous determination of seven taxoids in rat plasma by UPLC-MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts.
abstract: A rapid, sensitive and reliable method has been developed and validated for the simultaneous determination of seven taxoids including 10-deacetylbaccatin III (10-DAB III), baccatin III, 5-epi-canadensene, taxinine M, 10-deacetyltaxol (10-DAT), cephalomannine and paclitaxel in rat plasma using docetaxel as the internal standard (IS). The plasma samples were pretreated by liquid-liquid extraction with methyl tert-butyl ether. The chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 1.8 μm, Waters, USA) with a gradient elution program consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Detection was performed under the selected reaction monitoring (SRM) scan using an electrospray ionization (ESI) in the positive ion mode. The mass transitions were as follows: m/z 567.4→444.9 for 10-DAB III, m/z 609.0→549.3 for baccatin III, m/z 617.4→496.9 for 5-epi-canadensene, m/z 709.6→649.3 for taxinine M, m/z 834.8→307.9 for 10-DAT, m/z 854.5→285.4 for cephalomannine, m/z 876.8→307.3 for paclitaxel and m/z 830.8→549.6 for IS, respectively. All calibration curves exhibited good linearity (r(2)>0.99) over a wide concentration range for all components. The intra-day and inter-day precisions at three different levels were both less than 14.3% in terms of relative standard deviation (RSD) and the accuracies ranged from -8.3% to 14.8% in terms of relative error (RE). The extraction recoveries of the seven compounds ranged from 62.5% to 100.5%. The developed method was successfully applied to the pharmacokinetic study of the seven taxoids in rat plasma after oral administration of the crude extract of the twigs and leaves of Taxus yunnanensis.
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Bin
authors: B
authors: Liu
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Xiaoli
authors: X
authors: Gou
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Xupeng
authors: X
authors: Bai
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Xiangyu
authors: X
authors: Hou
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Dongshun
authors: D
authors: Li
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China.
authors: Guoping
authors: G
authors: Zhong
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China. Electronic address: jinjing@mail.sysu.edu.cn.
authors: Jing
authors: J
authors: Jin
authors: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, China. Electronic address: huangmin@mail.sysu.edu.cn.
authors: Min
authors: M
authors: Huang
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood / Q000737:chemistry; D000818:Animals; D001952:Bridged-Ring Compounds / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000077143:Docetaxel; D008297:Male; D008738:Methyl Ethers / Q000097:blood / Q000737:chemistry; D017239:Paclitaxel / Q000097:blood / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000493:pharmacokinetics*; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D043823:Taxoids / Q000097:blood* / Q000737:chemistry*; D020946:Taxus / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D001952:Bridged-Ring Compounds; D008738:Methyl Ethers; D010936:Plant Extracts; D043823:Taxoids; C071771:taxinine M; D000077143:Docetaxel; C043243:methyl tert-butyl ether; C073950:baccatin III; C042213:10-deacetylbaccatine III; C056348:cephalomannine; C031494:10-deacetyltaxol; D017239:Paclitaxel
keywords: Pharmacokinetics; Plasma concentration; Taxoids; Taxus yunnanensis; UPLC–MS/MS