title: Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion.
abstract: After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.
authors: Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.
authors: Chenxu
authors: C
authors: Li
keywords: Buyang Huanwu decoction; brain; cell membrane fluidity; electron spin resonance; free radical; ischemia/reperfusion; neural regeneration
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