title: Osteosarcoma tissues and cell lines from patients with differing serum alkaline phosphatase concentrations display minimal differences in gene expression patterns.
abstract: Serum alkaline phosphatase (ALP) concentration is a prognostic factor for osteosarcoma in multiple studies, although its biological significance remains incompletely understood. To determine whether gene expression patterns differed in osteosarcoma from patients with differing serum ALP concentrations, microarray analysis was performed on 18 primary osteosarcoma samples and six osteosarcoma cell lines from dogs with normal and increased serum ALP concentration. No differences in gene expression patterns were noted between tumours or cell lines with differing serum ALP concentration using a gene-specific two-sample t-test. Using a more sensitive empirical Bayes procedure, defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was increased in both the tissue and cell lines of the normal ALP group. Using quantitative PCR (qPCR), differences in DCUN1D1 expression between the two groups failed to reach significance. The homogeneity of gene expression patterns of osteosarcoma associated differing serum ALP concentrations are consistent with previous studies suggesting serum ALP concentration is not associated with intrinsic differences of osteosarcoma cells.
authors: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors: L C de Sá
authors: LC
authors: Rodrigues
authors: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors: K E
authors: KE
authors: Holmes
authors: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors: V
authors: V
authors: Thompson
authors: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors: C M
authors: CM
authors: Piskun
authors: Flint Animal Cancer Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft Collins, CO, USA.
authors: S E
authors: SE
authors: Lana
authors: Departments of Statistics and of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI, USA.|Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA.
authors: M A
authors: MA
authors: Newton
authors: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.|Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA.|Institute for Clinical & Translational Research, University of Wisconsin-Madison, Madison, WI, USA.
authors: T J
authors: TJ
authors: Stein
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000671:Amputation, Surgical / Q000662:veterinary; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism / Q000628:therapy / Q000662:veterinary*
chemical_list: D000970:Antineoplastic Agents; D000469:Alkaline Phosphatase
keywords: cell signalling; comparative oncology; genetics; oncology; tumour biology
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