title: The effects of Smad6 via small RNA pathways on the ischemic injury in PC12 cells.
abstract: BACKGROUND
  Though we have reported the neuroprotective effect of exogenous ActA on oxygen-glucose deprivation (OGD) injury, the endogenous role of Smad6 remains not well understood. Smad6 is an important regulator of the ActA/smads signaling via a negative feedback circuit.
  
  
  METHODS
  In this study, nerve growth factor (NGF) and OGD were used to stimulate (rat adrenal pheochromocytoma) PC12 cells converting them into neurons to establish an ischemia in vitro model. Combined with the small interfering technology of Smad6 and FCM, Hoechst and Western blot were used to identify the apoptosis rate. The effect of silencing of Smad6 with siRNA was observed.
  
  
  RESULTS
  These results showed that the apoptosis rate was 21.54% by 16-h OGD. For the combined Smad6-siRNA, the apoptosis rate was 10.55%.
  
  
  CONCLUSIONS
  The expression of procaspase-3 protein was increased by Smad6-siRNA.The expression of ActA and p300 was also increased. The apoptosis rate was decreased in the ischemic injury with Smad6-siRNA. At the same time, it provided a reference to study the mechanism of Smad6 and its signaling in response to the acute ischemic damage.
authors: Chun-Li
authors: CL
authors: Mei
authors: Li-Juan
authors: LJ
authors: Zhang
authors: Zhen-Wei
authors: ZW
authors: Fan
authors: Yan
authors: Y
authors: Song
authors: Min
authors: M
authors: Zhang
authors: Hui-Min
authors: HM
authors: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D053148:Caspase 3 / Q000378:metabolism; D015687:Cell Hypoxia; D049109:Cell Proliferation; D002470:Cell Survival; D005947:Glucose / Q000172:deficiency; D028322:Inhibin-beta Subunits / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D016716:PC12 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D051905:Smad6 Protein / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D050880:p300-CBP Transcription Factors / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D051905:Smad6 Protein; C497804:Smad6 protein, rat; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits; D050880:p300-CBP Transcription Factors; C505410:Casp3 protein, rat; D053148:Caspase 3; D005947:Glucose