title: Identification of H-type BSE in Portugal.
abstract: During the bovine spongiform encephalopathy (BSE) epidemic, Portugal was the third most affected country. As a result of a successful national eradication plan, the number of BSE affected animals has been progressively declining in Portugal with no cases identified in 2013. However, within the scope of this active surveillance scheme, we have identified the first H-type BSE case born after the introduction of the reinforced ban in fallen stock. Here, we report the phenotypic features of this case and the analysis of the protein coding sequence of prnp as well as the prnp promoter and intron 1 insertion-deletions.
authors: a Laboratório de Patologia polos Benfica e Vairão; Instituto Nacional de Investigação Agrária e Veterinária, IP ; Lisboa e Vairão , Portugal.
authors: Leonor
authors: L
authors: Orge
authors: Carla Guedes
authors: CG
authors: Machado
authors: Luísa
authors: L
authors: Ramalho
authors: Renata
authors: R
authors: Carvalho
authors: João
authors: J
authors: Silva
authors: Paula
authors: P
authors: Almeida
authors: Paula
authors: P
authors: Tavares
authors: Cristina
authors: C
authors: Ochoa
authors: Carla
authors: C
authors: Lima
authors: Maria J Marques
authors: MJ
authors: Pinto
authors: J Pedro
authors: JP
authors: Simas
mesh_terms: D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000145:classification* / Q000453:epidemiology / Q000235:genetics*; D054643:INDEL Mutation / Q000235:genetics; D007438:Introns / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011174:Portugal / Q000453:epidemiology; D011328:Prions / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D011328:Prions
keywords: BARB, born after the reinforced ban of meat and bone meal; BSE, bovine spongiform encephalopathy; EU, European Union; H-BSE; MBM, meat and bone meal; OIE, Office International of Epizooties; Portugal; PrP, prion protein; PrPres; PrPres, protease resistant prion protein; aa, amino acid; prnp; prnp, prion protein gene
references: Vet Pathol. 2013 Jul;50(4):659-63
references: 23269348
references: Vet Rec. 1987 Oct 31;121(18):419-20
references: 3424605
references: Vet Rec. 1994 Oct 22;135(17):400-3
references: 7856030
references: Prion. 2007 Jan-Mar;1(1):61-8
references: 19164888
references: J Clin Microbiol. 2012 Dec;50(12):4171-4
references: 23035195
references: Prev Vet Med. 2011 Nov 1;102(2):112-7
references: 21592603
references: BMC Res Notes. 2011 Sep 29;4:376
references: 21958476
references: EMBO Rep. 2004 Jan;5(1):110-5
references: 14710195
references: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3065-70
references: 14970340
references: J Anim Sci. 2007 Dec;85(12):3142-7
references: 17709775
references: BMC Vet Res. 2012;8:22
references: 22401036
references: Nature. 1997 Oct 2;389(6650):448-50, 526
references: 9333232
references: PLoS One. 2008;3(3):e1830
references: 18350166
references: J Neuropathol Exp Neurol. 2013 Mar;72(3):211-8
references: 23399901
references: PLoS One. 2010;5(5):e10638
references: 20498835
references: BMC Vet Res. 2012;8:74
references: 22647660
references: Neurogenetics. 2004 Feb;5(1):19-25
references: 14727152
references: Vet Rec. 1988 Dec 17;123(25):638-44
references: 3218047
references: Brain Res. 2010 Sep 10;1351:13-22
references: 20654596
references: PLoS Pathog. 2006 Oct;2(10):e112
references: 17054396
references: J Vet Sci. 2012 Jun;13(2):127-37
references: 22705734
references: BMC Biol. 2006;4:33
references: 17014722
references: J Vet Diagn Invest. 2008 Jan;20(1):2-10
references: 18182501
references: PLoS Pathog. 2008;4(9):e1000156
references: 18787697
references: J Neurosci. 2004 Mar 3;24(9):2156-64
references: 14999066
references: Vet Rec. 2000 Oct 21;147(17):486-8
references: 11093402
references: Emerg Infect Dis. 2006 Jul;12(7):1125-8
references: 16836832
references: J Clin Microbiol. 2007 Jun;45(6):1821-9
references: 17442800
references: Emerg Infect Dis. 2011 Sep;17(9):1636-44
references: 21888788
references: PLoS One. 2011;6(1):e15839
references: 21264286
references: J Appl Genet. 2012 Aug;53(3):337-42
references: 22723200
references: BMC Genet. 2007;8:15
references: 17437640