title: Integrative taxonomy reveals a new species of Callisto (Lepidoptera, Gracillariidae) in the Alps.
abstract: Europe has one of the best-known Lepidopteran faunas in the world, yet many species are still being discovered, especially in groups of small moths. Here we describe a new gracillariid species from the south-eastern Alps, Callistobasistrigella Huemer, Deutsch & Triberti, sp. n. It shows differences from its sister species Callistocoffeella in morphology, the barcode region of the cytochrome c oxidase I gene and the nuclear gene histone H3. Both Callistobasistrigella and Callistocoffeella can co-occur in sympatry without evidence of admixture. Two Callistobasistrigella specimens show evidence of introgression. We highlight the importance of an integrative approach to delimit species, combining morphological and ecological data with mitochondrial and nuclear sequence data. Furthermore, in connection with this study, Ornixblandella Müller-Rutz, 1920, syn. n. is synonymized with Callistocoffeella (Zetterstedt, 1839).
authors: INRA, UR0633 Zoologie Forestière, F-45075 Orléans, France ; Sukachev Institute of Forest SB RAS, Akademgorodok 50/28, 660036, Krasnoyarsk, Russia ; Siberian Federal University, 79 Svobodny pr., 660041, Krasnoyarsk, Russia.
authors: Natalia
authors: N
authors: Kirichenko
authors: Naturwissenschaftliche Abteilung, Tiroler Landesmuseen Betriebsgesellschaft m.b.H., Feldstr. 11a, A-6020 Innsbruck, Austria.
authors: Peter
authors: P
authors: Huemer
authors: Bannberg 22, 9911 Assling, East Tyrol, Austria.
authors: Helmut
authors: H
authors: Deutsch
authors: Museo Civico di Storia Naturale, Lungadige Porta Vittoria 9, I37129, Verona, Italy.
authors: Paolo
authors: P
authors: Triberti
authors: INRA, UR0633 Zoologie Forestière, F-45075 Orléans, France ; Museum National d'Histoire Naturelle, UMR7205 ISYEB, F-75005 Paris, France.
authors: Rodolphe
authors: R
authors: Rougerie
authors: INRA, UR0633 Zoologie Forestière, F-45075 Orléans, France.
authors: Carlos
authors: C
authors: Lopez-Vaamonde
keywords: COI; DNA barcoding; contact zone; histone H3; leaf-mining moths; mitochondrial-nuclear discordance; new species
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