title: Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis.
abstract: Hydrogen sulfide (H2S) signaling has been considered a key regulator of plant developmental processes and defenses. In this study, we demonstrate that high levels of H2S inhibit auxin transport and lead to alterations in root system development. H2S inhibits auxin transport by altering the polar subcellular distribution of PIN proteins. The vesicle trafficking and distribution of the PIN proteins are an actin-dependent process. H2S changes the expression of several actin-binding proteins (ABPs) and decreases the occupancy percentage of F-actin bundles in the Arabidopsis roots. We observed the effects of H2S on F-actin in T-DNA insertion mutants of cpa, cpb and prf3, indicating that the effects of H2S on F-actin are partially removed in the mutant plants. Thus, these data imply that the ABPs act as downstream effectors of the H2S signal and thereby regulate the assembly and depolymerization of F-actin in root cells. Taken together, our data suggest that the existence of a tightly regulated intertwined signaling network between auxin, H2S and actin that controls root system development. In the proposed process, H2S plays an important role in modulating auxin transport by an actin-dependent method, which results in alterations in root development in Arabidopsis.
authors: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Honglei
authors: H
authors: Jia
authors: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Nangang District, Harbin 150000, China.
authors: Yanfeng
authors: Y
authors: Hu
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Tingting
authors: T
authors: Fan
authors: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Jisheng
authors: J
authors: Li
mesh_terms: D000199:Actins / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics*; D009154:Mutation; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D008840:Microfilament Proteins; D006862:Hydrogen Sulfide
references: Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1510-4
references: 8108438
references: Anal Biochem. 1977 May 1;79(1-2):394-405
references: 869184
references: EMBO J. 2010 Aug 18;29(16):2700-14
references: 20717140
references: Annu Rev Plant Biol. 2006;57:109-25
references: 16669757
references: Plant Cell. 2002 Feb;14(2):293-9
references: 11884675
references: Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18506-11
references: 22021439
references: Cell. 2009 Mar 20;136(6):1005-16
references: 19303845
references: Plant Physiol. 2000 Feb;122(2):481-90
references: 10677441
references: Plant Sci. 2012 Sep;193-194:110-9
references: 22794924
references: Nature. 2005 Jan 6;433(7021):39-44
references: 15635403
references: New Phytol. 2011 Sep;191(4):970-83
references: 21569034
references: Plant Physiol Biochem. 2014 Mar;76:44-51
references: 24463534
references: Plant Cell Physiol. 2003 Nov;44(11):1131-40
references: 14634149
references: J Integr Plant Biol. 2009 Dec;51(12):1086-94
references: 20021556
references: Dev Cell. 2012 Jun 12;22(6):1275-85
references: 22698285
references: Neurochem Int. 2010 Jan;56(1):3-10
references: 19703504
references: J Mol Biol. 2007 Feb 2;365(5):1350-8
references: 17134718
references: Ann Bot. 2005 Apr;95(5):707-35
references: 15749753
references: Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4489-94
references: 18337510
references: Development. 2004 Jan;131(2):389-400
references: 14681187
references: New Phytol. 2010 Dec;188(4):977-84
references: 20831717
references: Trends Plant Sci. 2009 Jul;14(7):399-408
references: 19559642
references: Cold Spring Harb Perspect Biol. 2010 Jan;2(1):a001594
references: 20182605
references: Plant Cell. 2006 Nov;18(11):3171-81
references: 17114355
references: Plant Physiol Biochem. 2013 May;66:105-17
references: 23500713
references: Plant Physiol. 2004 May;135(1):266-78
references: 15122028
references: Plant J. 2005 Apr;42(2):188-200
references: 15807782
references: FASEB J. 2004 Mar;18(3):557-9
references: 14734631
references: J Biol Chem. 2003 Nov 7;278(45):44832-42
references: 12947123
references: Science. 2009 Nov 27;326(5957):1208-12
references: 19965462
references: FEBS Lett. 2005 Oct 10;579(24):5399-5406
references: 16198350
references: Anal Biochem. 1977 Dec;83(2):346-56
references: 603028
references: Plant J. 2007 May;50(3):514-28
references: 17419848
references: Protoplasma. 2014 Jul;251(4):899-912
references: 24318675
references: Development. 1997 Jan;124(1):33-44
references: 9006065
references: J Exp Bot. 2009;60(6):1605-17
references: 19261922
references: EMBO J. 1987 Dec 20;6(13):3901-7
references: 3327686
references: Cell. 2003 Nov 26;115(5):591-602
references: 14651850
references: J Integr Plant Biol. 2008 Dec;50(12):1518-29
references: 19093970
references: PLoS One. 2013;8(4):e62048
references: 23614010
references: Plant Cell. 2009 Jun;21(6):1659-68
references: 19491238
references: Cell. 1999 Nov 24;99(5):463-72
references: 10589675
references: Mol Biol Cell. 2006 Apr;17(4):1946-58
references: 16436516
references: Biometals. 2012 Jun;25(3):617-31
references: 22538639
references: Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:257-288
references: 15012193
references: Science. 2006 May 12;312(5775):883
references: 16601151
references: Curr Biol. 2006 Jun 6;16(11):1123-7
references: 16677815
references: Nature. 2003 Nov 13;426(6963):147-53
references: 14614497
references: J Exp Bot. 2013 Apr;64(7):1953-66
references: 23567865
references: Plant Cell. 2004 May;16(5):1191-205
references: 15100399
references: Curr Biol. 2013 May 6;23(9):817-22
references: 23583551
references: Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8790-4
references: 18559858
references: Plant Cell Rep. 2013 Jan;32(1):149-60
references: 23052593
references: Plant Cell Environ. 2013 Aug;36(8):1564-72
references: 23489239
references: Plant Physiol. 1978 Feb;61(2):184-9
references: 16660257
references: J Biol Chem. 2013 Nov 8;288(45):32277-88
references: 24072702
references: Plant Cell. 2009 Mar;21(3):701-18
references: 19304937
references: Plant Cell. 2010 Aug;22(8):2749-67
references: 20807879