title: Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step.
abstract: Sclerotinia resistance was transferred into rapeseed from a wild relative of Brassica oleracea (B. incana) using hexaploids derived from crosses between B. incana and rapeseed as a bridge. A high level of resistance against Sclerotinia sclerotiorum has been documented in wild Brassica oleracea, but not in cultivated rapeseed (Brassica napus). To transfer sclerotinia resistance from a wild relative into rapeseed, a strategy was proposed using hexaploids (AACCCC) derived from crosses between the wild B. oleracea-related B. incana genotype 'C01' and the Chinese rapeseed variety 'Zhongshuang 9' as a bridge. Progenies (BC1F1) generated by backcrossing the hexaploid to 'Zhongshuang 9' could be generated with a high crossability (average 18.3 seeds per pod). Seventy-three individuals in BC1F1 were firstly screened for resistance with five molecular markers linked to the major resistance QTL on chromosome C09 in 'C01', and 11 individuals harboring resistance loci were selected to develop vegetative clones. Of these, five exhibited significantly higher resistance than 'Zhongshuang 9' and the most resistant individual was chosen to develop the BC1F2 progeny. Finally, five individual genotypes with nearly twofold higher resistance than 'Zhongshuang 9' were found among 100 BC1F2 individuals by using marker-assisted selection and resistance evaluation. Hereof, one rapeseed-type individual with 38 chromosomes and good self-fertility (15.0 ± 3.56 seeds/pod) was identified. Our results indicate that the proposed strategy is effective for transferring sclerotinia resistance from a wild relative of B. oleracea into rapeseed.
authors: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China, jiaqinmay@163.com.
authors: Jiaqin
authors: J
authors: Mei
authors: Yao
authors: Y
authors: Liu
authors: Dayong
authors: D
authors: Wei
authors: Benjamin
authors: B
authors: Wittkop
authors: Yijuan
authors: Y
authors: Ding
authors: Qinfei
authors: Q
authors: Li
authors: Jiana
authors: J
authors: Li
authors: Huafang
authors: H
authors: Wan
authors: Zaiyun
authors: Z
authors: Li
authors: Xianhong
authors: X
authors: Ge
authors: Martin
authors: M
authors: Frauen
authors: Rod J
authors: RJ
authors: Snowdon
authors: Wei
authors: W
authors: Qian
authors: Wolfgang
authors: W
authors: Friedt
mesh_terms: D001203:Ascomycota; D001937:Brassica / Q000235:genetics; D029688:Brassica napus / Q000235:genetics*; D001947:Breeding; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D005838:Genotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011123:Polyploidy
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