title: Expression of recombinant Arabian camel lactoferricin-related peptide in Pichia pastoris and its antimicrobial identification.
abstract: BACKGROUND
  Lactoferricin (LFcin) is a strong cationic peptide released from the N-terminus of lactoferrin by gastric pepsin digestion. LFcin has some important properties, including high antimicrobial activity. To date, lactoferricins have been isolated and characterised from various animal species, but not from camel. The aim of this study was to characterise and express recombinant camel lactoferricin (LFcinC) in Pichia pastoris and investigate its antimicrobial activity.
  
  
  RESULTS
  After methanol induction, LFcinC was expressed and secreted into a culture broth medium and the results determined by concentrated supernatant culture medium showed high antimicrobial activity against the following microorganisms: Escherichia coli PTCC 1330 (ATCC 8739), Staphylococcus aureus PTCC 1112 (ATCC 6538), Pseudomonas aeruginosa PTCC 1074 (ATCC 9027), Bacillus subtilis PTCC 1023 (ATCC 6633), and Candida albicans PTCC 5027 (ATCC 10231). Thermal stability was clarified with antibacterial activity against Escherichia coli PTCC 1330 (ATCC 8739).
  
  
  CONCLUSIONS
  Results confirmed that camel lactoferricin had suitable antimicrobial activity and its production by Pichia pastoris can be used for recombinant production.
authors: Institute of Biotechnology, Shiraz University, Islamic Republic of Iran.
authors: Mahmood
authors: M
authors: Chahardooli
authors: Biotechnology Center, College of Agriculture, Shiraz University, Islamic Republic of Iran.
authors: Ali
authors: A
authors: Niazi
authors: Institute of Biotechnology, Shiraz University, Islamic Republic of Iran.
authors: Farzaneh
authors: F
authors: Aram
authors: Institute of Biotechnology, Shiraz University, Islamic Republic of Iran.
authors: Seyyed Mohsen
authors: SM
authors: Sohrabi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001483:Base Sequence; D002162:Camelus; D002176:Candida albicans / Q000187:drug effects; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D015870:Gene Expression; D006358:Hot Temperature; D007781:Lactoferrin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000494:pharmacology; D010843:Pichia / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012529:Saudi Arabia; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D010455:Peptides; D011994:Recombinant Proteins; C082171:lactoferricin B; D007781:Lactoferrin
keywords: Pichia pastoris; antimicrobial activity; camel lactoferricin; thermal stability