title: Artemisinin resistance-associated polymorphisms at the K13-propeller locus are absent in Plasmodium falciparum isolates from Haiti.
abstract: Antimalarial drugs are a key tool in malaria elimination programs. With the emergence of artemisinin resistance in southeast Asia, an effort to identify molecular markers for surveillance of resistant malaria parasites is underway. Non-synonymous mutations in the kelch propeller domain (K13-propeller) in Plasmodium falciparum have been associated with artemisinin resistance in samples from southeast Asia, but additional studies are needed to characterize this locus in other P. falciparum populations with different levels of artemisinin use. Here, we sequenced the K13-propeller locus in 82 samples from Haiti, where limited government oversight of non-governmental organizations may have resulted in low-level use of artemisinin-based combination therapies. We detected a single-nucleotide polymorphism (SNP) at nucleotide 1,359 in a single isolate. Our results contribute to our understanding of the global genomic diversity of the K13-propeller locus in P. falciparum populations.
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida tamarec@ufl.edu.
authors: Tamar E
authors: TE
authors: Carter
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Alexis
authors: A
authors: Boulter
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Alexandre
authors: A
authors: Existe
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Jean R
authors: JR
authors: Romain
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Jean Yves
authors: JY
authors: St Victor
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Connie J
authors: CJ
authors: Mulligan
authors: Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; National Public Health Laboratory, Ministry of Public Health and the Population, Port-au-Prince, Haiti; Hospital Saint Croix, Leogane, Haiti; Blanchard Clinic, Terre Noire, Haiti; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department of Environmental and Global Health, University of Florida, Gainesville, Florida.
authors: Bernard A
authors: BA
authors: Okech
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology*; D004351:Drug Resistance; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000453:epidemiology / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000187:drug effects*; D011110:Polymorphism, Genetic
chemical_list: D000962:Antimalarials; D037621:Artemisinins; C031327:artemisinin
references: N Engl J Med. 2014 Jul 31;371(5):411-23
references: 25075834
references: N Engl J Med. 2009 Jul 30;361(5):455-67
references: 19641202
references: Am J Trop Med Hyg. 2012 Jan;86(1):29-31
references: 22232446
references: N Engl J Med. 2008 Dec 11;359(24):2619-20
references: 19064625
references: Malar J. 2012;11:275
references: 22889367
references: Nature. 2014 Jan 2;505(7481):50-5
references: 24352242
references: Emerg Infect Dis. 2012 Sep;18(9):1487-9
references: 22932030
references: PLoS One. 2014;9(8):e105690
references: 25144768
references: Malar J. 2013;12:426
references: 24252305
references: Lancet. 2005 Dec 3;366(9501):1960-3
references: 16325698
references: Malar J. 2013;12:30
references: 23347639
references: Science. 2010 May 14;328(5980):850-1
references: 20466921
references: J Infect Dis. 2015 Mar 1;211(5):680-8
references: 25180240
references: PLoS One. 2013;8(3):e57689
references: 23520478
references: Med Sante Trop. 2012 Oct-Dec;22(4):435-9
references: 23353074
references: Lancet. 2012 May 26;379(9830):1960-6
references: 22484134
references: Malar J. 2014;13:361
references: 25218803
references: Bull World Health Organ. 1984;62(4):623-6
references: 6386210
references: Bull Soc Pathol Exot. 2015 Feb;108(1):14-6
references: 24903032
references: Antimicrob Agents Chemother. 2012 May;56(5):2546-52
references: 22354307
references: PLoS One. 2014;9(4):e93684
references: 24691467
references: PLoS One. 2013;8(3):e59192
references: 23555629