title: Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes.
abstract: B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Hai-Nan
authors: HN
authors: Lan
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Hai-Long
authors: HL
authors: Jiang
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Wei
authors: W
authors: Li
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Tian-Cheng
authors: TC
authors: Wu
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Pan
authors: P
authors: Hong
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Yu Meng
authors: YM
authors: Li
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Hui
authors: H
authors: Zhang
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Huan-Zhong
authors: HZ
authors: Cui
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China .
authors: Xin
authors: X
authors: Zheng
keywords: Janus Kinase (2)-Signal Transducers and Activators of Transcription [JAK2-STAT5]; Monoclonal Anti-idiotypic Antibodies (Mab2s); Porcine Growth Hormone; Porcine Hepatocytes
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