title: Denitrifying sulfide removal by Pseudomonas sp. C27 at excess carbon supply: mechanisms.
abstract: Pseudomonas sp. C27 can effectively conduct mixotrophic denitrifying sulfide removal (DSR) reactions using both organic matters and sulfide as electron donors. This study conducted DSR tests using C27 and quantitatively analyzed the protein abundances at C/N=1.26, 1.63 and 3.0. At C/N=1.26, C27 principally adopted autotrophic denitrification pathway in DSR reaction. As C/N ratio was increased to 1.63, C27 enhanced heterotrophic denitrification pathway for removing nitrous compounds. As the C/N ratio was further increased to 3.0, C27 accelerated metabolism via coupled-cycles pathway. The C/N ratio for coupled-cycles pathway was estimated ranging 2.0-2.3 in the studied medium. Optimal C/N ratio of traditional DSR processes ranged 1.05-1.26. With the coupled-cycles pathway, the accessible C/N/S regime for C27 on DSR reactions is enlarged. Minor revision of the coupled-cycles pathway considering production of ammonium step was made.
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Hongliang
authors: H
authors: Guo
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Chuan
authors: C
authors: Chen
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan. Electronic address: djlee@ntu.edu.tw.
authors: Duu-Jong
authors: DJ
authors: Lee
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Aijie
authors: A
authors: Wang
authors: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors: Nanqi
authors: N
authors: Ren
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D058440:Denitrification; D009584:Nitrogen; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013440:Sulfides; D002244:Carbon; D009584:Nitrogen
keywords: C/N ratio; DSR; Nitrate; Pseudomonas sp. C27; Sulfide