title: Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.
abstract: Dystrophic cardiomyopathy is a poorly understood consequence of muscular dystrophy. Generating induced Pluripotent Stem Cells (iPSCs) from patients with muscular dystrophy is an invaluable cellular source for in vitro disease model systems and can be used for drug screening studies. Patient-derived urine cells have been used in successful reprogramming into induced pluripotent stem cells in order to model dystrophic cardiomyopathy(1). Addressing the safety concerns of integrating vector systems, we present a protocol using a non-integrating Sendai virus vector for transduction of Yamanaka factors into urine cells collected from patients with muscular dystrophy. This protocol generates fully reprogrammed clones within 2-3 weeks. The pluripotent cells are vector-free by passage-13. These dystrophic iPSCs can be differentiated into cardiomyocytes and used either to study disease mechanisms or for drug screening.
authors: Department of Medicine, Medical College of Wisconsin.
authors: Muhammad Z
authors: MZ
authors: Afzal
authors: Department of Medicine, Medical College of Wisconsin; jstrande@mcw.edu.
authors: Jennifer L
authors: JL
authors: Strande
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D009136:Muscular Dystrophies / Q000473:pathology* / Q000652:urine*; D032383:Myocytes, Cardiac / Q000166:cytology; D029082:Sendai virus / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods
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