title: Photo-crosslinkable biopolymers targeting stem cell adhesion and proliferation: the case study of gelatin and starch-based IPNs.
abstract: The present work focuses on the development of biomaterials that support the adhesion and the proliferation of adipose-tissue derived stem cells. Therefore, gelatin and starch are selected as starting materials. Both hydrogel building blocks are of great interest as they provide a general chemical structure comparable to the protein and the polysaccharide constituting part of the extracellular matrix. Crosslinkable side groups are incorporated on both biopolymers to enable the subsequent chemical crosslinking, thereby ensuring their stability at physiological temperature. An in vitro cellular assay revealed that the hydrogels developed are biocompatible and supported cell adhesion of adipose-tissue derived mesenchymal stem cells. The presence of the starch phase tempered the adhesion resulting in local cell detachment. The results thus indicate that by carefully varying the ratio of the two building blocks, hydrogels can be developed possessing a controllable cell adhesion behavior.
authors: Polymer Chemistry & Biomaterials Group, Ghent University, Krijgslaan 281, S4-Bis, 9000, Ghent, Belgium.
authors: Ine
authors: I
authors: Van Nieuwenhove
authors: Sandra
authors: S
authors: Van Vlierberghe
authors: Achim
authors: A
authors: Salamon
authors: Kirsten
authors: K
authors: Peters
authors: Hugo
authors: H
authors: Thienpont
authors: Peter
authors: P
authors: Dubruel
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D019245:Compressive Strength; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000633:toxicity; D004058:Diffusion; D005780:Gelatin / Q000737:chemistry* / Q000633:toxicity; D006244:Hardness; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000633:toxicity; D008027:Light; D008422:Materials Testing; D008795:Metronidazole / Q000008:administration & dosage / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods; D013213:Starch / Q000737:chemistry* / Q000633:toxicity; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D008795:Metronidazole; D005780:Gelatin; D013213:Starch
references: J Mater Sci Mater Med. 2008 Apr;19(4):1459-66
references: 18299964
references: Biomaterials. 2012 Jan;33(1):48-58
references: 21955690
references: Biomacromolecules. 2011 May 9;12(5):1387-408
references: 21388145
references: Pharm Res. 1997 Jul;14(7):840-1
references: 9244137
references: Biomaterials. 2003 Nov;24(24):4337-51
references: 12922147
references: J Biomed Mater Res A. 2012 Feb;100(2):470-6
references: 22125000
references: Nature. 2010 Aug 12;466(7308):829-34
references: 20703299
references: Biomacromolecules. 2007 Feb;8(2):331-7
references: 17291055
references: Nat Mater. 2011 Sep 23;10(10):727-9
references: 21941268
references: Trends Biotechnol. 1998 May;16(5):224-30
references: 9621462
references: Appl Spectrosc. 2010 Oct;64(10):1176-80
references: 20925989
references: Materials (Basel). 2014 Feb 19;7(2):1342-1359
references: 28788517
references: Stem Cells Transl Med. 2012 Jun;1(6):510-9
references: 23197855
references: Macromol Rapid Commun. 2014 Aug;35(15):1351-5
references: 24942823
references: Adv Drug Deliv Rev. 1998 Aug 3;33(1-2):165-182
references: 10837658
references: J Pediatr Surg. 2012 Jan;47(1):17-28
references: 22244387
references: Cell. 2007 Oct 19;131(2):324-36
references: 17956733
references: Biomacromolecules. 2007 Feb;8(2):338-44
references: 17291056
references: Macromol Biosci. 2009 Nov 10;9(11):1105-15
references: 19626719