title: Novel localization of peripherin 2, the photoreceptor-specific retinal degeneration slow protein, in retinal pigment epithelium.
abstract: Retinal pigment epithelium (RPE) builds the outer blood-retinal barrier of the eye. Since one typical feature of the autoimmune disease, equine recurrent uveitis (ERU), is the breakdown of this barrier, we recently performed comparative analysis of healthy and uveitic RPE. We identified for the first time peripherin 2, which is responsible for visual perception and retina development, to be localized in RPE. The purpose of this study was therefore to validate our findings by characterizing the expression patterns of peripherin 2 in RPE and retina. We also investigated whether peripherin 2 expression changes in ERU and if it is expressed by the RPE itself. Via immunohistochemistry, significant downregulation of peripherin 2 in uveitic RPE compared to the control was detectable, but there was no difference in healthy and uveitic retina. A further interesting finding was the clear distinction between peripherin 2 and the phagocytosis marker, rhodopsin, in healthy RPE. In conclusion, changes in the expression pattern of peripherin 2 selectively affect RPE, but not retina, in ERU. Moreover, peripherin 2 is clearly detectable in healthy RPE due to both phagocytosis and the expression by the RPE cells themselves. Our novel findings are very promising for better understanding the molecular mechanisms taking place on RPE in uveitis.
authors: Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. p.uhl@tiph.vetmed.uni-muenchen.de.
authors: Patrizia B
authors: PB
authors: Uhl
authors: Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. baerbl.amann@tiph.vetmed.uni-muenchen.de.
authors: Barbara
authors: B
authors: Amann
authors: Research Unit for Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany. hauck@helmholtz-muenchen.de.
authors: Stefanie M
authors: SM
authors: Hauck
authors: Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. deeg@tiph.vetmed.uni-muenchen.de.
authors: Cornelia A
authors: CA
authors: Deeg
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D006736:Horses; D006801:Humans; D007150:Immunohistochemistry; D064531:Peripherins / Q000378:metabolism*; D010587:Phagocytosis; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism; D014605:Uveitis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary
chemical_list: D064531:Peripherins; D012243:Rhodopsin
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