title: Analysis of by-product formation and sugar monomerization in sugarcane bagasse pretreated at pilot plant scale: differences between autohydrolysis, alkaline and acid pretreatment.
abstract: Sugarcane bagasse is an interesting feedstock for the biobased economy since a large fraction is polymerized sugars. Autohydrolysis, alkaline and acid pretreatment conditions combined with enzyme hydrolysis were used on lignocellulose rich bagasse to acquire monomeric. By-products found after pretreatment included acetic, glycolic and coumaric acid in concentrations up to 40, 21 and 2.5 g/kg dry weight bagasse respectively. Alkaline pretreated material contained up to 45 g/kg bagasse DW of sodium. Acid and autohydrolysis pretreatment results in a furan formation of 14 g/kg and 25 g/kg DW bagasse respectively. Enzyme monomerization efficiencies of pretreated solid material after 72 h were 81% for acid pretreatment, 77% for autohydrolysis and 57% for alkaline pretreatment. Solid material was washed with superheated water to decrease the amount of by-products. Washing decreased organic acid, phenol and furan concentrations in solid material by at least 60%, without a major sugar loss.
authors: Food and Biobased Research, Wageningen University and Research Center, PO Box 17, 6700 AA Wageningen, The Netherlands; Bioprocess Engineering, Wageningen University and Research Center, PO Box 16, 6700 AA Wageningen, The Netherlands. Electronic address: edwin.vanderpol@wur.nl.
authors: Edwin
authors: E
authors: van der Pol
authors: Food and Biobased Research, Wageningen University and Research Center, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors: Rob
authors: R
authors: Bakker
authors: Food and Biobased Research, Wageningen University and Research Center, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors: Alniek
authors: A
authors: van Zeeland
authors: Corbion Purac Biochem, PO Box 21, 4200 AA Gorinchem, The Netherlands.
authors: David
authors: D
authors: Sanchez Garcia
authors: Laboratory of Food Chemistry, Wageningen University and Research center, Bornse Weilanden 9, 6708 WG, The Netherlands.
authors: Arjen
authors: A
authors: Punt
authors: Food and Biobased Research, Wageningen University and Research Center, PO Box 17, 6700 AA Wageningen, The Netherlands; Bioprocess Engineering, Wageningen University and Research Center, PO Box 16, 6700 AA Wageningen, The Netherlands.
authors: Gerrit
authors: G
authors: Eggink
mesh_terms: D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000096:biosynthesis*; D002264:Carboxylic Acids / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005663:Furans / Q000032:analysis; D006868:Hydrolysis; D007477:Ions; D013058:Mass Spectrometry; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D010636:Phenols / Q000032:analysis; D010865:Pilot Projects; D031786:Saccharum / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013464:Sulfuric Acids / Q000494:pharmacology*
chemical_list: D002241:Carbohydrates; D002264:Carboxylic Acids; D005663:Furans; D007477:Ions; D008670:Metals; D010636:Phenols; D013464:Sulfuric Acids; D012972:Sodium Hydroxide; D002482:Cellulose; C027433:bagasse; C033158:sulfuric acid
keywords: Analysis; By-products; Inhibitors; Lignocellulose; Pretreatment