title: Association of IL-13 single nucleotide polymorphisms in Iranian patients to multiple sclerosis.
abstract: MS is an autoimmune disease and interleukin 13 (IL-13) has been proposed to be an important neuroprotective mediator in MS. Because of plausible effect of single nucleotide polymorphisms (SNPs) in expression level or biological activity of any cytokine, we sought to investigate association of IL-13 SNPs, C-1112T, A-1512C and G+2044A, with risk to MS. Sixty-eight RRMS patients and 110 healthy controls were involved in this study. After extraction of genomic DNA, frequency of genotypes and alleles were determined by PCR-RFLP and data were analyzed statistically. Results showed significant higher frequency of CC, CC, and AA genotypes and C, C, and A alleles of -1112CT, -1512AC and +2044GA SNPs respectively, in patients group. There was significant association between -1112C allele with onset age of MS. No significant association was seen between any of genotypes or alleles with expanded disability status scale (EDSS) of patients. Our findings showed significant association between three studied SNPs of IL-13 with susceptibility to MS in Iranian patients. More studies should be done on other IL-13 SNPs, and also polymorphisms of IL-13 receptor and other cytokines to determine the exact role of SNPs in protecting or predisposing of individuals for MS.
authors: Neuroscience Research Center, Imam Reza Teaching Hospital, School of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Narges
authors: N
authors: Seyfizadeh
authors: Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Tohid
authors: T
authors: Kazemi
authors: Neuroscience Research Center, Imam Reza Teaching Hospital, School of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Mehdi
authors: M
authors: Farhoudi
authors: Immunology Unit, Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Mohammad Reza
authors: MR
authors: Aliparasti
authors: Department of Statistics & Epidemiology, Faculty of Health & Nutrition Faculty, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Homayoun
authors: H
authors: Sadeghi-Bazargani
authors: Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Shohreh
authors: S
authors: Almasi
authors: Neuroscience Research Center, Imam Reza Teaching Hospital, School of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran ; Immunology Unit, Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors: Zohreh
authors: Z
authors: Babaloo
keywords: Multiple sclerosis; PCR; RFLP; interleukin 13; polymorphism
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