title: Wastewater treatment and biodiesel production by Scenedesmus obliquus in a two-stage cultivation process.
abstract: The microalga Scenedesmus obliquus was cultured in two cultivation stages: (1) in batch with real wastewater; (2) maintaining the stationary phase with different conditions of CO2, light and salinity according to a factorial design in order to improve the lipid content. The presence of the three factors increased lipid content from 35.8% to 49% at the end of the second stage; CO2 presence presented the highest direct effect increasing lipid content followed by light presence and salt presence. The ω-3 fatty acids content increased with CO2 and light presence acting in isolation, nevertheless, when both factors acted together the interaction effect was negative. The ω-3 eicosapentaenoic acid content of the oil from S. obliquus slightly exceeded the 1% maximum to be used as biodiesel source (EU normative). Therefore, it is suggested the blend with other oils or the selective extraction of the ω-3 fatty acids from S. obliquus oil.
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain. Electronic address: pablo.alvarez@uca.es.
authors: P D
authors: PD
authors: Álvarez-Díaz
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain.
authors: J
authors: J
authors: Ruiz
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain; FCC Aqualia, Avenida Camino de Santiago 40, 28050 Madrid, Spain.
authors: Z
authors: Z
authors: Arbib
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain; Chiclana Natural S.A.M., Pza. de España S.N., 11130 Chiclana, Cádiz, Spain.
authors: J
authors: J
authors: Barragán
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain.
authors: M C
authors: MC
authors: Garrido-Pérez
authors: Department of Environmental Technologies, Andalusian Center of Science and Marine Technology (CACYTMAR), International Campus of Excellence of the Sea (CEIMAR), Universidad de Cádiz, Campus of Puerto Real, 11510 Puerto Real, Cádiz, Spain.
authors: J A
authors: JA
authors: Perales
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D008027:Light; D054712:Salinity; D044444:Scenedesmus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology; D062065:Wastewater; D018508:Water Purification / Q000379:methods*
chemical_list: D056804:Biofuels; D002245:Carbon Dioxide; D005227:Fatty Acids; D012965:Sodium Chloride; D062065:Wastewater
keywords: Biodiesel; Fatty acid; Lipid; Scenedesmus obliquus; Wastewater