title: Disturbances of modulating molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA expressions in the orbital tissue from patients with severe graves' ophthalmopathy.
abstract: Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves' orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n = 6) or severe GO (n = 20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R = 0.796, P = 0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO.
authors: Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, Poland ; Department of Pediatric Ophthalmology with Strabismus Treatment Unit, Medical University of Białystok, 17 Waszyngtona Street, 15-274 Białystok, Poland.
authors: Przemyslaw
authors: P
authors: Pawlowski
authors: Department of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
authors: Natalia
authors: 0000-0002-7071-0783
authors: N
authors: Wawrusiewicz-Kurylonek
authors: Department of Ophthalmology, Essen University Hospital, University of Duisburg-Essen, Hufeland Straße 55, 45 122 Essen, Germany.
authors: Anja
authors: A
authors: Eckstein
authors: Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, Poland.
authors: Joanna
authors: 0000-0002-0169-0897
authors: J
authors: Reszec
authors: Department of Pediatrics, Endocrinology and Diabetology with Cardiology Division, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Białystok, Poland.
authors: Wlodzimierz
authors: W
authors: Luczynski
authors: Bayer HealthCare, 10 Kaiser-Wilhelm-Allee, 51373 Leverkusen, Germany.
authors: Kristian
authors: K
authors: Johnson
authors: Department of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
authors: Adam
authors: A
authors: Kretowski
authors: Department of Pediatric Ophthalmology with Strabismus Treatment Unit, Medical University of Białystok, 17 Waszyngtona Street, 15-274 Białystok, Poland.
authors: Alina
authors: A
authors: Bakunowicz-Lazarczyk
authors: Department of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
authors: Maria
authors: M
authors: Gorska
authors: Department of Gynaecology and Oncologic Gynaecology, Medical University of Bialystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
authors: Jacek
authors: J
authors: Szamatowicz
authors: Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, Poland.
authors: Lech
authors: L
authors: Chyczewski
authors: Department of Nuclear Medicine, Medical University of Bialystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, Poland.
authors: Janusz
authors: J
authors: Mysliwiec
mesh_terms: D000328:Adult; D060887:B7 Antigens / Q000235:genetics*; D018106:CD28 Antigens / Q000235:genetics*; D017252:CD3 Complex / Q000378:metabolism; D019013:CD40 Antigens / Q000235:genetics*; D023201:CD40 Ligand / Q000235:genetics*; D060908:CTLA-4 Antigen / Q000235:genetics*; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D049970:Graves Ophthalmopathy / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D060887:B7 Antigens; D018106:CD28 Antigens; D017252:CD3 Complex; D019013:CD40 Antigens; D060908:CTLA-4 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D023201:CD40 Ligand
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