title: The inhibition of Platycodin D on Mycoplasma pneumoniae proliferation and its effect on promoting cell growth after anti-Mycoplasma pneumoniae treatment.
abstract: Platycodin D, extract from the root of Platycodon grandiflorum, is one of the most important monomers of the Qinbaiqingfei pellets (Qinbai) that has already been approved as the first Traditional Chinese Medicine for clinic use as an anti-M. pneumoniae agent. Qinbai constituents Scutellaria baicalensis and Platycodon grandiflorum were used to treat thousands of patients clinically in China each year. In this study, a M. pneumoniae-infected mouse strain, BALB/c, and a human-derived epithelial cell line, A549 type II pneumocytes, were used as experimental model. Anti-M. pneumoniae effect of Platycodin D was measured by the Real-time quantitative PCR, while the cell pathological change with hematoxylin and eosin and the growth recovery effects were determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Trypan Blue dye in the experimental model after M. pneumoniae infection. Our research results showed that Platycodin D could significantly inhibit M. pneumoniae and promote cell growth after anti- M. pneumoniae treatment in the infected cells or mice.
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Yanli
authors: Y
authors: Meng
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Yang
authors: Y
authors: Yang
authors: School of Food Science and Engineering, Harbin Institute of Technology Harbin, China.
authors: Weihong
authors: W
authors: Lu
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Yingyan
authors: Y
authors: Wang
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Feng
authors: F
authors: Qian
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Xin
authors: X
authors: Wang
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Zhihua
authors: Z
authors: Zhang
authors: Heilongjiang Academy of Chinese Medicine Sciences Harbin, China.
authors: Weiming
authors: W
authors: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009177:Mycoplasma pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D031238:Platycodon / Q000737:chemistry*; D011019:Pneumonia, Mycoplasma / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology; D012503:Saponins / Q000008:administration & dosage*; D014315:Triterpenes / Q000008:administration & dosage*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C108953:platycodin D
keywords: A549 cells; M. pneumoniae; Platycodin D; cell growth; mouse models
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