title: Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette.
abstract: Host DNA may adversely affect metagenomic studies focusing on the prokaryotic microbiota. This study compared the levels of host DNA in subgingival plaque collected by paper points and curette, using quantitative PCR. Lower proportions of host DNA and higher proportions of bacterial DNA were recovered from samples collected with curettes.
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil. Electronic address: julianaperezch@gmail.com.
authors: P J
authors: PJ
authors: Pérez-Chaparro
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: P M
authors: PM
authors: Duarte
authors: Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
authors: C M
authors: CM
authors: Pannuti
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: L C
authors: LC
authors: Figueiredo
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: M J
authors: MJ
authors: Mestnik
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: C P S
authors: CP
authors: Gonçalves
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: M
authors: M
authors: Faveri
authors: Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
authors: M
authors: M
authors: Feres
mesh_terms: D055113:Chronic Periodontitis / Q000382:microbiology; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D003773:Dental Plaque / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D056186:Metagenomics; D064307:Microbiota; D010209:Paper; D060888:Real-Time Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D004247:DNA
keywords: Dental plaque; High-Throughput Nucleotide Sequencing; Microbiota; Quantitative Real-Time Polymerase Chain Reaction