title: Analysis of fatty acid samples by hydrophilic interaction liquid chromatography and charged aerosol detector.
abstract: HILIC/CAD techniques were used in analysis of samples containing fatty acids. Amine base column appeared to be the more retentive stationary phase compared to zwitterionic and BEH silica. The retention decreased with pH mobile phases changing from 3 to 5. Acetonitrile and acetone organic modifier were compared. Acetone gave higher eluotropic strength and better peak symmetry whereas acetonitrile led to higher efficiency. The retention decreased when ammonium acetate concentration increased from 5 to 20mM. The use of sub-2μm column did not show flat Van Deemter curves at high flow rates. A rapid separation of PGI2 and its main degradation product, 6-keto prostaglandin F1α was obtained in 1.6min with a Hypersil GOLD, 50mm×2.1mm, 1.9μm with; acetonitrile/acetate ammonium pH 5 at 20mM (85/15; v/v at 0.7ml/min).
authors: EA 4575 Analytical and Pharmaceutical Developments Applied to Neglected Diseases and Counterfeits, Université de Bordeaux, 146, rue Léo Saignat, France.
authors: Claude-Eric
authors: CE
authors: Roy
authors: EA 4575 Analytical and Pharmaceutical Developments Applied to Neglected Diseases and Counterfeits, Université de Bordeaux, 146, rue Léo Saignat, France.
authors: Tina
authors: T
authors: Kauss
authors: EA 4575 Analytical and Pharmaceutical Developments Applied to Neglected Diseases and Counterfeits, Université de Bordeaux, 146, rue Léo Saignat, France.
authors: Sandrine
authors: S
authors: Prevot
authors: ChemBioMed, U869 INSERM, Université de Bordeaux, 146, rue Léo Saignat, France.
authors: Philippe
authors: P
authors: Barthelemy
authors: EA 4575 Analytical and Pharmaceutical Developments Applied to Neglected Diseases and Counterfeits, Université de Bordeaux, 146, rue Léo Saignat, France. Electronic address: Karen.gaudin@u-bordeaux.fr.
authors: Karen
authors: K
authors: Gaudin
mesh_terms: D000336:Aerosols / Q000737:chemistry*; D000527:Alprostadil / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002846:Chromatography, Affinity; D011464:Epoprostenol / Q000032:analysis* / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics
chemical_list: D000336:Aerosols; D005227:Fatty Acids; C022950:6-ketoprostaglandin E1; D011464:Epoprostenol; D000527:Alprostadil
keywords: CAD; Fatty acid; HILIC; PGI2; Prostacyclin; Prostaglandin