title: In vitro assessment of platelet lesions during 5-day storage in Iranian Blood Transfusion Organization (IBTO) centers.
abstract: OBJECTIVE
  Platelet concentrates (PC) are used in thrombocytopenia and inherited or acquired platelet dysfunction disorders. Thus, retaining the platelets quality and function during storage will lead to desirable outcomes in treatment of such patients.
  
  
  METHODS
  In this study, we evaluated 40 PC bags, prepared by PRP method in IBTO centers. We applied an array of assays, on first, third and fifth days of storage for PC quality control, including swirling, cell counting, bacterial contamination, measurement of CD62P, pH, and platelet aggregation test, to evaluate platelet lesion during storage.
  
  
  RESULTS
  All units were negative for bacterial contamination. Swirling was positive for all units on various days; platelet count was in the acceptable range. Measurement of CD62P on fifth day was not significantly higher than third or first day (P > 0.15) (P > 0.05). pH on fifth day was significantly lower than first day (P < 0.01) (P < 0.05). Platelet aggregation with arachidonic acid and ristocetin showed significant decrease on fifth day compared to third day (P < 0.01) (P < 0.05).
  
  
  CONCLUSIONS
  CD62P associated with other platelet function tests can be used as an activation marker in evaluation of PC functions during storage.
authors: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. gharehbaghian@sbmu.ac.ir.
authors: Behruz
authors: B
authors: Ghezelbash
authors: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors: Sedigheh
authors: S
authors: Amini Kafiabad
authors: Mazandaran University of Medical Sciences, Mazandaran, Iran.
authors: Mohammad Taher
authors: MT
authors: Hojjati
authors: Shahid Beheshti University of medical sciences, Tehran, Iran.
authors: Mohsen
authors: M
authors: Hamidpoor
authors: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors: Shahram
authors: S
authors: Vaeli
authors: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors: Mohammad Reza
authors: MR
authors: Tabtabae
authors: Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Ahmad
authors: A
authors: Gharehbaghian
mesh_terms: D015415:Biomarkers; D001792:Blood Platelets / Q000166:cytology* / Q000382:microbiology; D001793:Blood Preservation; D001803:Blood Transfusion; D003430:Cross-Sectional Studies; D006801:Humans; D007492:Iran; D019007:P-Selectin / Q000378:metabolism*; D010974:Platelet Aggregation; D010976:Platelet Count / Q000592:standards*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019007:P-Selectin; C546937:SELP protein, human