title: Metformin down-regulates endometrial carcinoma cell secretion of IGF-1 and expression of IGF-1R.
abstract: As metformin can inhibit endometrial carcinoma (EC) cell growth and the insulin growth factor (IGF) system is active in EC, the question of whether t can regulate endometrial carcinoma cell secretion of IGF-1 or expression of IGF-1 receptor (IGF-1R) is of interest. In this study, serum IGF-1 levels in EC patients were found to be comparable with that in the non EC patients (p>0.05). However, the IGF-1 level in the medium of cultured cells after treatment with metformin was decreased (p<0.05). IGF-1R was highly expressed in human endometrial carcinoma paraffin sections, but IGF-1R and phosphor-protein kinase B/protein kinase B (p-Akt/ Akt) expression was down-regulated after metformin treatment (p<0.05). In summary, metformin can reduce the secretion of IGF-1 by Ishikawa and JEC EC cell lines and their expression of IGF-1R to deactivate downstream signaling involving the PI-3K/Akt pathway to inhibit endometrial carcinoma cell growth.
authors: Department of Gynecology The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, PR China E-mail : zhyuchenchen@163.com, tigerlee777@163.com.
authors: Yu
authors: Y
authors: Zhang
authors: Meng-Xiong
authors: MX
authors: Li
authors: Huan
authors: H
authors: Wang
authors: Zheng
authors: Z
authors: Zeng
authors: Xiao-Mao
authors: XM
authors: Li
mesh_terms: D002277:Carcinoma / Q000188:drug therapy* / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000235:genetics; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008687:Metformin / Q000494:pharmacology*; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D017526:Receptor, IGF Type 1 / Q000235:genetics* / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D007334:Insulin-Like Growth Factor I; D008687:Metformin; D019869:Phosphatidylinositol 3-Kinases; D017526:Receptor, IGF Type 1; D051057:Proto-Oncogene Proteins c-akt