title: In vitro induction of human adipose-derived stem cells into lymphatic endothelial-like cells.
abstract: Human adipose-derived stem cells (hADSCs) may provide a suitable number of progenitors for the treatment of lymphatic edema; however, to date the protocols for inducing hADSCs into this tissue type have not been standardized. We wished to investigate the induction of hADSCs into lymphatic endothelial-like cells using vascular endothelial growth factor-C156S (VEGF-C156S) and other growth factors in vitro. hADSCs from healthy adult adipose tissue were purified using enzyme digestion. Differentiation was induced using medium containing VEGF-C156S and bovine fibroblast growth factor (bFGF). Differentiation was confirmed using immunostaining for lymphatic vessel endothelial hyaluronan receptor (LYVE-1) and fms-related tyrosine kinase 4 (FLT-4), two lymphatic endothelial cell markers. The expression levels of LYVE-1, prospero homeobox 1 (PROX-1), and FLT-4 throughout induction were assessed using reverse transcriptase quantitative polymerase chain reaction. hADSCs were successfully obtained by trypsin digest and purification. Flow cytometry showed these cells were similar to mesenchymal stem cells, with a high positive rate of CD13, CD29, CD44, and CD105, and a low positive rate of CD31, CD34, CD45, and HLA-DR. Induction to lymphatic endothelial-like cells was successful, with cells expressing high levels of LYVE-1, PROX-1, and FLT-4. Adipose-derived stem cells can be induced to differentiate into lymphatic endothelial-like cells using a medium containing VEGF-C156S, bFGF, and other growth factors. This population of lymphatic endothelial-like cells may be useful for lymphatic reconstruction in the future.
authors: 1 Department of Microsurgery and Orthopedic Trauma, The First Affiliated Hospital of Sun Yat-sen University , Guangzhou, 510080, China .
authors: Yi
authors: Y
authors: Yang
authors: Xiao-hu
authors: XH
authors: Chen
authors: Fu-gui
authors: FG
authors: Li
authors: Yun-xian
authors: YX
authors: Chen
authors: Li-qiang
authors: LQ
authors: Gu
authors: Jia-kai
authors: JK
authors: Zhu
authors: Ping
authors: P
authors: Li
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D042582:Vascular Endothelial Growth Factor C / Q000494:pharmacology*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000378:metabolism; D000098551:Prospero-Related Homeobox 1 Protein
chemical_list: D015703:Antigens, CD; D018398:Homeodomain Proteins; C119248:LYVE1 protein, human; D025521:Tumor Suppressor Proteins; D042582:Vascular Endothelial Growth Factor C; D033921:Vesicular Transport Proteins; D000098551:Prospero-Related Homeobox 1 Protein; C581309:FLT4 protein, human; D040321:Vascular Endothelial Growth Factor Receptor-3