title: Preliminary evidence for a serum disaccharide signature of invasive Candida albicans infection detected by MALDI Mass Spectrometry.
abstract: The diagnosis of systemic Candida infections is a recognized challenge. We developed a mass spectrometry strategy to detect signals from Candida molecules in patients' sera. Pre-analytical procedures were designed to extract oligosaccharides from serum. A peak m/z of at 365 was specifically revealed in sera from patients with candidaemia with regard to healthy controls. This biomarker was identified as a disaccharide, its presence did not correlate with mannanaemia or glucanaemia. Mouse models of Candida albicans colonization and infection showed that the signal was specifically associated with tissue invasion, suggesting that clinical evaluation of its usefulness in discriminating colonized and infected patients would be worthwhile.
authors: Université de Lille, Lille, France; Service de Parasitologie-Mycologie, CHRU, Lille, France; INSERM 995, Lille, France.
authors: B
authors: B
authors: Sendid
authors: Université de Lille, Lille, France; INSERM 995, Lille, France; Pôle de Réanimation CHRU, Lille, France.
authors: J
authors: J
authors: Poissy
authors: Service de Parasitologie-Mycologie, CHRU, Lille, France; INSERM 995, Lille, France.
authors: N
authors: N
authors: François
authors: SATT Nord-de-France, Lille, France.
authors: A
authors: A
authors: Mery
authors: Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille, Villeneuve d'Ascq, France; CNRS, UMR 8576, Villeneuve d'Ascq, France.
authors: S
authors: S
authors: Courtecuisse
authors: Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille, Villeneuve d'Ascq, France; CNRS, UMR 8576, Villeneuve d'Ascq, France; PAGés, CNRS FR3637, Université Lille1, Villeneuve d'Ascq, France.
authors: F
authors: F
authors: Krzewinski
authors: Université de Lille, Lille, France; INSERM 995, Lille, France.
authors: S
authors: S
authors: Jawhara
authors: Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille, Villeneuve d'Ascq, France; CNRS, UMR 8576, Villeneuve d'Ascq, France.
authors: Y
authors: Y
authors: Guerardel
authors: Université de Lille, Lille, France; Service de Parasitologie-Mycologie, CHRU, Lille, France; INSERM 995, Lille, France; Délégation à la Recherche Clinique et à l'Innovation, CHRU, Lille, France. Electronic address: dpoulain@univ-lille2.fr.
authors: D
authors: D
authors: Poulain
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002176:Candida albicans; D058365:Candidiasis, Invasive / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D004187:Disaccharides / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009172:Mycology / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D015415:Biomarkers; D004187:Disaccharides
keywords: Candida albicans; diagnosis; disaccharide; invasive fungal infections; mass spectrometry; matrix-assisted laser desorption/ionization time-of flight