title: Identification and genetic characterization by high-throughput SNP analysis of intervarietal substitution lines of rapeseed (Brassica napus L.) with enhanced embryogenic potential.
abstract: Seven intervarietal substitution lines were identified with embryogenic potentials up to 40.4 times that of the recurrent parent, providing an ideal material for further in depth studies of this trait. To identify genomic regions that carry genetic factors controlling embryogenic potential of isolated microspores of rapeseed, marker segregations were analysed in a segregating population of haploid microspore-derived embryos and a BC1 population from a cross between 'Express 617' and 'RS239'. After map construction 15 intervarietal substitution lines from the same cross with 'Express 617' as recurrent parent were selected with donor segments covering five genomic regions that had shown skewed segregations in the population of microspore-derived embryos but not in the BC1 population. By comparing the embryogenic potential of microspores of the 15 substitution lines and 'Express 617', seven lines were identified with significantly enhanced embryogenic potential ranging from 4.1 to 40.4 times that of 'Express 617'. To improve the genetic characterization of the selected lines, they were subjected to a high-throughput SNP analysis using the Illumina Infinium 60K chip for rapeseed. Based on 7,960 mapped SNP markers, one to eight donor segments per line, which cover 0.64-6.79% of the 2,126.1 cM of the SNP map, were found. The SNP analysis also gave evidence that homoeologous exchanges had occurred during the development of the substitution line population, increasing the genetic diversity within this population. By comparing donor segments between lines with significantly enhanced embryogenic potential and non-significant lines, 12 genomic regions were identified that may contain genetic factors controlling embryogenic potential in rapeseed. These regions range in size from 0 (represented by just one marker) to 26.8 cM and cover together just 5.42% of the SNP map.
authors: Department of Crop Sciences, Georg-August-Universität Göttingen, Von-Siebold-Str. 8, 37075, Göttingen, Germany, wecke@gwdg.de.
authors: Wolfgang
authors: W
authors: Ecke
authors: Anthimos
authors: A
authors: Kampouridis
authors: Katharina
authors: K
authors: Ziese-Kubon
authors: Ann-Catrin
authors: AC
authors: Hirsch
mesh_terms: D029688:Brassica napus / Q000196:embryology* / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
references: Plant Physiol. 2007 May;144(1):134-54
references: 17384168
references: Theor Appl Genet. 1995 Feb;90(2):194-204
references: 24173891
references: Theor Appl Genet. 2010 Mar;120(5):921-31
references: 19953219
references: Theor Appl Genet. 1995 Nov;91(6-7):841-7
references: 24169967
references: New Phytol. 2010 Apr;186(1):102-12
references: 20149113
references: Science. 2014 Aug 22;345(6199):950-3
references: 25146293
references: Theor Appl Genet. 2006 Dec;114(1):67-80
references: 17033785
references: Genome. 1995 Dec;38(6):1112-21
references: 18470235
references: Theor Appl Genet. 2006 Nov;113(7):1331-45
references: 16960716
references: Genetics. 2005 Feb;169(2):967-79
references: 15520255
references: Genome. 1995 Dec;38(6):1122-31
references: 18470236
references: Theor Appl Genet. 1994 Nov;89(5):615-21
references: 24177938
references: Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7719-23
references: 7644483
references: Genome. 1996 Apr;39(2):348-58
references: 18469898
references: Plant Cell Rep. 1998 Mar;17(5):323-328
references: 30736566
references: Genetics. 2008 Jul;179(3):1547-58
references: 18562665