title: The role of antioxidant activity in the prevention and treatment of infertility caused by Cisplatin in rats.
abstract: OBJECTIVE
  To investigate the importance of antioxidant activity in infertility caused by cisplatin in rats.
  
  
  METHODS
  Rats in cisplatin control (CG), Vitamin E + cisplatin (ECG), Vitamin C + cisplatin (CCG), Hippophae rhamnoides extract (HRE) + cisplatin (HRECG), and thiamine pyrophosphate (TPP) + cisplatin (TPPCG) groups were injected intraperitoneally (ip) with (100 mg/kg) Vitamin E, Vitamin C, HRE, and TPP, respectively. One hour later, ip cisplatin was administered (5 mg/kg), and then antioxidant medications were continued for 10 days. Cisplatin + Vitamin E (CEG-1), cisplatin + Vitamin C (CCG-1), cisplatin + HRE (CHREG-1), and cisplatin + TPP (TPPCG-1) rats received cisplatin (5 mg/kg, ip) and were kept for 10 days. At the end of that period, rats received antioxidant medications for 10 days. (n = 12, for each group). Six rats from each group were sacrificed. Ovaries were removed to measure malondialdehyde, total glutathione, glutathione S-transferase, and glutathione reductase levels. The remaining rats were kept in a suitable laboratory environment.
  
  
  RESULTS
  Cisplatin-induced oxidative stress was best prevented by HRE, Vitamin E, Vitamin C, and TPP, in that order. However, infertility caused by cisplatin was only prevented and treated by TPP.
  
  
  CONCLUSIONS
  Oxidative stress is not a major component in the pathogenesis of cisplatin-associated infertility.
authors: Department of Obsterics and Gynecology, Nenehatun Hospital, Erzurum, Turkey.
authors: Ayse Nur
authors: AN
authors: Aksoy
authors: Suna
authors: S
authors: Kabil Kucur
authors: Gonca
authors: G
authors: Batmaz
authors: Ilay
authors: I
authors: Gözükara
authors: Mehmet
authors: M
authors: Aksoy
authors: Nezahat
authors: N
authors: Kurt
authors: Renad
authors: R
authors: Mammadov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D007247:Infertility, Female / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002945:Cisplatin