title: Parameters of dynamic contrast-enhanced MRI as imaging markers for angiogenesis and proliferation in human breast cancer.
abstract: BACKGROUND
  Breast cancer is the most common malignancy and the leading cause of cancer death in women worldwide; however, early diagnosis has been difficult due to its complex pathological structure. This study evaluated the value of morphological examination in conjunction with dynamic contrast-enhanced MRI (DCE-MRI) for more precise diagnosis of breast cancer, as well as their correlation with angiogenesis and proliferation biomarkers.
  
  
  METHODS
  DCE-MRI parameters (including Ktrans: volume transfer coefficient reflecting vascular permeability, Kep: flux rate constant, Ve: extracellular volume ratio reflecting vascular permeability, and ADC: apparent diffusion coefficient) were obtained from 124 patients with breast cancer (124 lesions). Microvessel density (MVD) was evaluated by the immunohistochemical analysis of tumor vessels for CD31 and CD105 expression. The proliferation was assessed by analyzing Ki67.
  
  
  RESULTS
  Ktrans values were in the order of: malignant lesions>benign lesions>normal glands. Similar results were observed for Kep. The opposite changes were seen with Ve. Ktrans and Kep values were significantly higher in invasive ductal carcinoma (IDC) and ductal carcinoma in situ (DCIS) than in mammary ductal dysplasia (MDD; ANOVA followed by Dunnett's test). In sharp contrast, ADC values were lower in IDC and DCIS than in MDD, and Ve was not significantly different among the three groups. The data from MIP (maximum intensity projection) showed that benign breast lesions had no or only one blood vessel, whereas malignant lesions had two or more blood vessels. In addition, expression of CD105 and Ki67, the commonly recognized markers for angiogenesis and proliferation, respectively, were closely correlated with MRI parameters as revealed by Pearson analysis.
  
  
  CONCLUSIONS
  Determination of Ktrans, Kep and ADC values permits estimation of tumor angiogenesis and proliferation in breast cancer and DCE-MRI parameters can be used as imaging biomarkers to predict patient prognosis and the biologic aggressiveness of the tumor.
authors: Department of Radiology, Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Lin
authors: L
authors: Li
authors: Molecular Imaging Center, Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Kai
authors: K
authors: Wang
authors: Molecular Imaging Center, Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Xilin
authors: X
authors: Sun
authors: Molecular Imaging Center, Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Kezheng
authors: K
authors: Wang
authors: Molecular Imaging Center, Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Yingying
authors: Y
authors: Sun
authors: Department of Radiology, Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Guangfeng
authors: G
authors: Zhang
authors: Department of Radiology, Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Baozhong
authors: B
authors: Shen
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D003287:Contrast Media / Q000737:chemistry*; D000071063:Endoglin; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*; D010539:Permeability; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D003287:Contrast Media; C064206:ENG protein, human; D000071063:Endoglin; D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface
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