title: In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice.
abstract: The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0 T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.
authors: Neuroimmunology and Neuromuscular Diseases Unit, Fondazione IRCCS Istituto Neurologico 'Carlo Besta', Milan, Italy.
authors: Laura
authors: L
authors: Turati
authors: Marco
authors: M
authors: Moscatelli
authors: Alfonso
authors: A
authors: Mastropietro
authors: Nicholas G
authors: NG
authors: Dowell
authors: Ileana
authors: I
authors: Zucca
authors: Alessandra
authors: A
authors: Erbetta
authors: Chiara
authors: C
authors: Cordiglieri
authors: Greta
authors: G
authors: Brenna
authors: Beatrice
authors: B
authors: Bianchi
authors: Renato
authors: R
authors: Mantegazza
authors: Mara
authors: M
authors: Cercignani
authors: Fulvio
authors: F
authors: Baggi
authors: Ludovico
authors: L
authors: Minati
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D003471:Cuprizone; D005260:Female; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging; D008297:Male; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000378:metabolism / Q000473:pathology*
chemical_list: D003471:Cuprizone
keywords: animal model; cuprizone; demyelination; macromolecular pool size ratio (F); multiple sclerosis; quantitative magnetization transfer imaging (qMT); remyelination