title: Maresin-1 reduces airway inflammation associated with acute and repetitive exposures to organic dust.
abstract: Agriculture industry workers are at a higher risk for chronic bronchitis and obstructive pulmonary diseases, and current therapeutics are not entirely effective. We previously found that the specialized proresolving lipid mediator maresin-1 (MaR1) reduced proinflammatory cytokine release and intracellular adhesion molecule-1 (ICAM-1) expression in bronchial epithelial cells exposed to extracts of organic dust (DE) derived from swine confinement facilities in vitro. The objective of this study was to determine whether MaR1 is effective at limiting lung inflammation associated with acute and repetitive exposures to DE in an established murine model of inhalant dust exposures. C57Bl/6 mice were treated with MaR1 or vehicle control and intranasally instilled with DE once or daily for 3 weeks. Bronchioalveolar lavage fluid was analyzed for total and differential cell counts and proinflammatory cytokine levels, and lung tissues were assessed for histopathology and ICAM-1 expression. In both single and repetitive DE exposure studies, MaR1 significantly decreased bronchoalveolar lavage neutrophil infiltration, interleukin 6, tumor necrosis factor α, and chemokine C-X-C motif ligand 1 levels without altering repetitive DE-induced bronchioalveolar inflammation or lymphoid aggregate formation. Lung tissue ICAM-1 expression was also reduced in both single and repetitive exposure studies. These data suggest that MaR1 might contribute to an effective strategy to reduce airway inflammatory diseases induced by agricultural-related organic dust environmental exposures.
authors: Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb.
authors: Tara M
authors: TM
authors: Nordgren
authors: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Neb.
authors: Christopher D
authors: CD
authors: Bauer
authors: Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb.
authors: Art J
authors: AJ
authors: Heires
authors: Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb.
authors: Jill A
authors: JA
authors: Poole
authors: VA Nebraska-Western Iowa Health Care System, Research Service and Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb; Department of Environmental, Agricultural, and Occupational Health, University of Nebraska Medical Center, Omaha, Neb.
authors: Todd A
authors: TA
authors: Wyatt
authors: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Neb.
authors: William W
authors: WW
authors: West
authors: Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb; VA Nebraska-Western Iowa Health Care System, Research Service and Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb. Electronic address: dromberg@unmc.edu.
authors: Debra J
authors: DJ
authors: Romberger
mesh_terms: D000382:Agricultural Workers' Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D000395:Air Pollutants, Occupational / Q000009:adverse effects; D000822:Animal Husbandry; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004391:Dust; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D009930:Organic Chemicals / Q000009:adverse effects; D052638:Particulate Matter / Q000009:adverse effects; D011014:Pneumonia / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D034421:Sus scrofa; D057170:Translational Research, Biomedical
chemical_list: C535211:7,14-dihydroxydocosa-4,8,10,12,16,19-hexaenoic acid; D000395:Air Pollutants, Occupational; D016207:Cytokines; D004391:Dust; D009930:Organic Chemicals; D052638:Particulate Matter; D018799:Intercellular Adhesion Molecule-1; D004281:Docosahexaenoic Acids
references: PLoS One. 2013;8(3):e58258
references: 23484005
references: Nutrients. 2014 Dec;6(12):5434-52
references: 25436433
references: J Immunol. 2013 Oct 15;191(8):4288-98
references: 24038091
references: Br J Pharmacol. 2014 Jul;171(14):3539-50
references: 24697684
references: Respir Res. 2013;14:51
references: 23663457
references: Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16526-31
references: 25369934
references: Environ Health Perspect. 2000 Aug;108 Suppl 4:705-12
references: 10931789
references: Eur Respir J. 2000 Sep;16(3):404-8
references: 11028651
references: J Appl Physiol (1985). 2002 Jul;93(1):289-96
references: 12070216
references: Scand J Work Environ Health. 2002 Aug;28(4):256-63
references: 12199427
references: J Agric Saf Health. 2003 Aug;9(3):185-96
references: 12970949
references: J Appl Physiol (1985). 2004 May;96(5):1738-44
references: 14729721
references: Am J Ind Med. 2004 Oct;46(4):357-62
references: 15376214
references: Am J Respir Cell Mol Biol. 1992 Aug;7(2):214-21
references: 1353976
references: Am J Respir Cell Mol Biol. 1993 Dec;9(6):586-93
references: 7504927
references: Am J Respir Crit Care Med. 1996 Sep;154(3 Pt 1):602-11
references: 8810593
references: Am J Respir Crit Care Med. 1997 Jan;155(1):254-9
references: 9001321
references: Eur Respir J. 1997 Feb;10(2):381-7
references: 9042636
references: Am J Respir Crit Care Med. 1998 Nov;158(5 Pt 2):S1-S76
references: 9817727
references: Occup Med. 1999 Apr-Jun;14(2):337-50
references: 10329909
references: Respir Med. 2006 May;100(5):846-54
references: 16214322
references: Curr Opin Pharmacol. 2006 Aug;6(4):414-20
references: 16750421
references: Biochem Biophys Res Commun. 2008 Mar 7;367(2):509-15
references: 18190790
references: J Agric Saf Health. 2008 Apr;14(2):189-203
references: 18524284
references: J Periodontol. 2008 Aug;79(8 Suppl):1520-6
references: 18673006
references: J Immunol. 2010 Jan 15;184(2):836-43
references: 20007539
references: Curr Top Med Chem. 2011;11(6):629-47
references: 21261595
references: Trends Neurosci. 2011 Nov;34(11):599-609
references: 21963090
references: Ann Agric Environ Med. 2011;18(2):310-3
references: 22216804
references: J Nutr. 2012 Mar;142(3):587S-591S
references: 22323763
references: Prostaglandins Other Lipid Mediat. 2012 Mar;97(3-4):73-82
references: 22326554
references: FASEB J. 2012 Apr;26(4):1755-65
references: 22253477
references: N C Med J. 2011 Nov-Dec;72(6):477-80
references: 22523860
references: Br J Nutr. 2012 Jun;107 Suppl 2:S85-106
references: 22591907
references: J Clin Lipidol. 2012 May-Jun;6(3):216-34
references: 22658146
references: BMC Med. 2012;10:50
references: 22613931
references: J Immunol. 2012 Aug 15;189(4):1983-91
references: 22802419
references: Ann Allergy Asthma Immunol. 2012 Oct;109(4):266-273.e2
references: 23010233
references: Am J Respir Cell Mol Biol. 2012 Nov;47(5):652-9
references: 22822029
references: Respir Res. 2012;13:110
references: 23199346
references: Blood. 2013 Jun 13;121(24):4930-7
references: 23645836
references: J Allergy Clin Immunol. 2008 Aug;122(2):375-82, 382.e1-4
references: 18585769
references: Intensive Care Med. 2008 Sep;34(9):1580-92
references: 18461305
references: JPEN J Parenter Enteral Nutr. 2008 Nov-Dec;32(6):596-605
references: 18974237
references: J Exp Med. 2009 Jan 16;206(1):15-23
references: 19103881
references: Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L1085-95
references: 19395665
references: Chest. 2009 Sep;136(3):716-25
references: 19318669