title: Use of conditioned medium for efficient transformation and cost-effective cultivation of Nannochloropsis salina.
abstract: The oleaginous microalga Nannochloropsis sp. has been spotlighted as a promising candidate in genetic engineering research for biodiesel production. However, one of the major bottlenecks in the genetic manipulation against Nannochloropsis sp. is low transformation efficiency. Based on the idea that they grow rapidly in broth culture, the effect of conditioned medium on colonization and transformation efficiency of Nannochloropsis salina was investigated. Cells grown on agar plates with 20-40% conditioned medium produced colonies that were approximately 2.3-fold larger than cells grown without conditioned medium. More importantly, the transformation efficiency was about 2-fold greater on plates with 30% conditioned medium relative to those without conditioned medium. In addition, FAME productivity in liquid cultures with 100% conditioned medium increased up to 20% compared with cultures of control medium. These results suggest that conditioned medium can be applied for efficient transformation and cost-effective cultivation of N. salina for biodiesel production.
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Nam Kyu
authors: NK
authors: Kang
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Bongsoo
authors: B
authors: Lee
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Sung-Eun
authors: SE
authors: Shin
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Seungjib
authors: S
authors: Jeon
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Min S
authors: MS
authors: Park
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: jwyang@kaist.ac.kr.
authors: Ji-Won
authors: JW
authors: Yang
mesh_terms: D001761:Bleomycin / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000191:economics* / Q000379:methods*; D003362:Cost-Benefit Analysis; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004952:Esters / Q000032:analysis; D015203:Reproducibility of Results; D058009:Stramenopiles / Q000187:drug effects / Q000254:growth & development*; D014170:Transformation, Genetic / Q000187:drug effects*; D019076:Transgenes
chemical_list: D017077:Culture Media, Conditioned; D004952:Esters; D001761:Bleomycin; C105427:Zeocin
keywords: Colonization; Conditioned medium; Fatty acid methyl ester (FAME); Nannochloropsis salina; Transformation efficiency