title: Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov., isolated from an ancient tree trunk, and emended description of the genus Pedobacter.
abstract: Two Gram-stain-negative, yellow, aerobic and rod-shaped bacterial isolates, designated THG-DN3.18(T) and THG-DN3.19(T), were isolated from an ancient tree trunk from Daejeon, South Korea. 16S rRNA gene sequence similarity showed that both strains belong to the genus Pedobacter within the family Sphingobacteriaceae . Strain THG-DN3.18(T) exhibited maximum sequence similarity with Pedobacter boryungensis KCTC 23344(T) (98.5%) while strain THG-DN3.19(T) exhibited maximum sequence similarity with Pedobacter nyackensis LMG 24260(T) (97.3%). In DNA-DNA hybridization tests, the two strains showed less than 35% relatedness with respect to closely related species of the genus Pedobacter . Both strains contained iso-C(15 : 0) and C(16 : 1)ω6c and/or C(16 : 1)ω7c (summed feature 3) as the predominant fatty acids and MK-7 as the major isoprenoid quinone. The DNA G+C contents of strains THG-DN3.18(T) and THG-DN3.19(T) were 35.5 and 40.1 mol%, respectively. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strains THG-DN3.18(T) and THG-DN3.19(T) represent novel species of the genus Pedobacter , for which the names Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov. are proposed. The type strains are THG-DN3.18(T) ( = KCTC 42230(T) = JCM 30352(T)) and THG-DN3.19(T) ( = KCTC 42233(T) = JCM 30353(T)), respectively.
authors: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Juan
authors: J
authors: Du
authors: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Hina
authors: H
authors: Singh
authors: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Hien T T
authors: HTT
authors: Ngo
authors: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Kyung-Hwa
authors: KH
authors: Won
authors: Department of Genetic Engineering, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Ki-Young
authors: KY
authors: Kim
authors: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Tae-Hoo
authors: TH
authors: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014197:Trees / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7