title: A gain-of-function mutation of plastidic invertase alters nuclear gene expression with sucrose treatment partially via GENOMES UNCOUPLED1-mediated signaling.
abstract: Plastid gene expression (PGE) is one of the signals that regulate the expression of photosynthesis-associated nuclear genes (PhANGs) via GENOMES UNCOUPLED1 (GUN1)-dependent retrograde signaling. We recently isolated Arabidopsis sugar-inducible cotyledon yellow-192 (sicy-192), a gain-of-function mutant of plastidic invertase, and showed that following the treatment of this mutant with sucrose, the expression of PhANGs as well as PGE decreased, suggesting that the sicy-192 mutation activates a PGE-evoked and GUN1-mediated retrograde pathway. To clarify the relationship between the sicy-192 mutation, PGE, and GUN1-mediated pathway, plastid and nuclear gene expression in a double mutant of sicy-192 and gun1-101, a null mutant of GUN1 was studied. Plastid-encoded RNA polymerase (PEP)-dependent PGE was markedly suppressed in the sicy-192 mutant by the sucrose treatment, but the suppression as well as cotyledon yellow phenotype was not mitigated by GUN1 disruption. Microarray analysis revealed that the altered expression of nuclear genes such as PhANG in the sucrose-treated sicy-192 mutant was largely dependent on GUN1. The present findings demonstrated that the sicy-192 mutation alters nuclear gene expression with sucrose treatment via GUN1, which is possibly followed by inhibiting PEP-dependent PGE, providing a new insight into the role of plastid sugar metabolism in nuclear gene expression.
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan.
authors: Takanori
authors: T
authors: Maruta
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.
authors: Nozomi
authors: N
authors: Miyazaki
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.
authors: Ryota
authors: R
authors: Nosaka
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.
authors: Hiroyuki
authors: H
authors: Tanaka
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.
authors: Daniel
authors: D
authors: Padilla-Chacon
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.
authors: Kumi
authors: K
authors: Otori
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.
authors: Ayako
authors: A
authors: Kimura
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.
authors: Noriaki
authors: N
authors: Tanabe
authors: Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.|Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan.
authors: Kazuya
authors: K
authors: Yoshimura
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.
authors: Masahiro
authors: M
authors: Tamoi
authors: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631-8505, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan.
authors: Shigeru
authors: S
authors: Shigeoka
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010788:Photosynthesis / Q000235:genetics; D018087:Plastids / Q000201:enzymology*; D015398:Signal Transduction; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C519677:GUN1 protein, Arabidopsis; D013395:Sucrose; D043324:beta-Fructofuranosidase; D009584:Nitrogen
keywords: Arabidopsis; genomes uncoupled 1; plastid gene expression; plastidic invertase; retrograde signaling
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