title: Analgesic effect of electroacupuncture on paclitaxel-induced neuropathic pain via spinal opioidergic and adrenergic mechanisms in mice.
abstract: This study was designed to determine the antinociceptive effect and related neuronal mechanism of electroacupuncture (EA) on paclitaxel (PTX)-induced neuropathic pain in mice. PTX (4 mg/kg, i.p.) was administered once a day for 5 consecutive days to induce neuropathic pain. EA stimulation (2 mA, 2 Hz, 30 min) was applied at the ST36 acupoint bilaterally once in every 2 days. Repeated EA stimulation significantly attenuated PTX-induced mechanical allodynia and thermal hyperalgesia. In a separate set of experiment, the antinociceptive effect of a single EA stimulation 8 days after PTX treatment was reduced by intrathecal pretreatment with naloxone (opioid receptor antagonist), idazoxan (alpha2-adrenoceptor antagonist) or propranolol (beta-adrenoceptor antagonist), but not prazosin (alpha1-adrenoceptor antagonist). Moreover, EA remarkably suppressed the PTX-enhanced phosphorylation of the NMDA receptor NR2B subunit in the spinal dorsal horn, and intrathecal pretreatment of naloxone, idazoxan (IDA) or propranolol blocked the effect of EA. In conclusion, EA stimulation at the ST36 acupoint significantly diminished PTX-induced neuropathic pain in mice via the mediation of spinal opioid receptor, alpha2- and beta-adrenoceptors.
authors: Department of Physiology and Brain Research Institute, Chungnam National University, School of Medicine, Daejeon 301-747, South Korea.
authors: Jung-Wan
authors: JW
authors: Choi
authors: Suk-Yun
authors: SY
authors: Kang
authors: Jae-Gyun
authors: JG
authors: Choi
authors: Dong-Wook
authors: DW
authors: Kang
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Sang Do
authors: SD
authors: Lee
authors: Jin Bong
authors: JB
authors: Park
authors: Yeon-Hee
authors: YH
authors: Ryu
authors: Hyun-Woo
authors: HW
authors: Kim
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000009:adverse effects*; D015671:Electroacupuncture; D008813:Mice, Inbred ICR; D009437:Neuralgia / Q000139:chemically induced* / Q000628:therapy; D017239:Paclitaxel / Q000008:administration & dosage / Q000009:adverse effects*; D010446:Peptide Fragments / Q000378:metabolism; D010766:Phosphorylation; D018341:Receptors, Adrenergic, alpha-2 / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D011957:Receptors, Opioid / Q000502:physiology*; D013116:Spinal Cord
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C485096:NR2B protein (1292-1308); D010446:Peptide Fragments; D018341:Receptors, Adrenergic, alpha-2; D011943:Receptors, Adrenergic, beta; D016194:Receptors, N-Methyl-D-Aspartate; D011957:Receptors, Opioid; D017239:Paclitaxel
keywords: Chemotherapy; Electroacupuncture; Neuropathic Pain; Paclitaxel; ST36