title: Protective effect of 940 nm laser on gamma-irradiated mice.
abstract: OBJECTIVE
  The purpose of this study was to investigate the radioprotective features of 940 nm laser on the life span of mice, and absolute counts of blood cells and their proportions in gamma-irradiated mice.
  
  
  BACKGROUND
  An important feature of laser light is activation of mitotic division and differentiation of cells, which may be useful in activation of hematopoiesis in gamma-irradiated organisms.
  
  
  METHODS
  Mice were randomly assigned to 11 groups according to the type(s) of influence. Generally, mice were irradiated in three different ways: with laser at different fluences, with gamma irradiation, or by combination of laser at different fluences and gamma irradiation in a different order. Mice were treated with 940 nm laser at 3, 12, or 18 J/cm(2) and/or a lethal dose of gamma irradiation (8.7 Gy). Each group was randomly subdivided into two subgroups, in which the life span of the mice and blood cell counts (on 12th and 45th day after gamma irradiation) were analyzed.
  
  
  RESULTS
  Laser (940 nm) at a fluence of 3 J/cm(2) significantly prolonged the life span of gamma-irradiated mice (p<0.05). In the same group, counts of white blood cells, lymphocytes, and neutrophils were higher on day 12 than in the gamma group. On day 45 after gamma irradiation, some signs of hematopoiesis repair were found in blood. There were no significant differences in counts of erythrocytes, monocytes, neutrophils, or the proportion of neutrophils between this group and the control group.
  
  
  CONCLUSIONS
  In summary, 940 nm laser at a fluence of 3 J/cm(2) demonstrates radioprotective features in an experiment with lethally irradiated mice. Mechanisms responsible for this effect will be investigated in further studies.
authors: 1 Department of Health Care Disciplines and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague , Kladno, Czech Republic .
authors: Yulia
authors: Y
authors: Efremova
authors: Zuzana
authors: Z
authors: Sinkorova
authors: Leos
authors: L
authors: Navratil
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D005260:Female; D005720:Gamma Rays; D054023:Lasers, Semiconductor; D008136:Longevity / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation
references: Radiat Res. 2003 May;159(5):567-80
references: 12710868
references: Radiat Res. 2003 May;159(5):581-96
references: 12710869
references: Lasers Surg Med. 2012 Nov;44(9):726-35
references: 23001637
references: Photomed Laser Surg. 2012 Oct;30(10):579-86
references: 22891782
references: Drug Discov Ther. 2012 Aug;6(4):169-77
references: 23006987
references: Photomed Laser Surg. 2012 Aug;30(8):405-17
references: 22747309
references: Lasers Med Sci. 2012 Mar;27(2):325-31
references: 21246387
references: Lasers Surg Med. 2011 Sep;43(8):851-9
references: 21956634
references: PLoS One. 2011;6(7):e22453
references: 21814580
references: Lasers Med Sci. 2011 Jan;26(1):125-31
references: 20865435
references: Photomed Laser Surg. 2009 Dec;27(6):863-9
references: 19697999
references: BMC Complement Altern Med. 2009;9:8
references: 19368718
references: Antioxid Redox Signal. 2014 Mar 20;20(9):1463-80
references: 24180340
references: Biol Res Nurs. 2014 Apr;16(2):191-6
references: 23559459
references: J Oral Pathol Med. 2014 Feb;43(2):103-10
references: 23952682
references: J Biomed Opt. 2013 Dec;18(12):128001
references: 24337495
references: J Biophotonics. 2013 Oct;6(10):829-38
references: 23281261
references: Photomed Laser Surg. 2013 Jul;31(7):315-21
references: 23789588
references: Lasers Med Sci. 2013 Jul;28(4):1169-76
references: 23053250
references: Lasers Med Sci. 2013 Feb;28(2):431-6
references: 22399243
references: Lasers Med Sci. 2013 Feb;28(2):383-90
references: 22354548
references: Photochem Photobiol. 2013 Jan-Feb;89(1):179-88
references: 22882462
references: Photomed Laser Surg. 2006 Feb;24(1):33-7
references: 16503786
references: J Biomed Sci. 2009;16:4
references: 19272168
references: Lasers Surg Med. 2009 Apr;41(4):282-90
references: 19347944