title: In vitro and in vivo evaluation of vancomycin-loaded PMMA cement in combination with ultrasound and microbubbles-mediated ultrasound.
abstract: Ultrasound (US) has been used to increase elution of antibiotic from an antibiotic-loaded poly(methyl methacrylate) (PMMA) bone cement (ALBC). We aimed to further investigate whether microbubbles-mediated US (US + MB) facilitate elution of vancomycin (VAN) from cylindrical specimens and enhance the activity of the eluted antibiotic against Staphylococcus aureus (S. aureus) in vitro. The study groups comprised cylindrical bone cement fabricated with VAN (VAN), ALBC using US (VAN + US), and ALBC using MB-mediated US (VAN + US + MB). We also carried out an in vivo study involving the activity of VAN from cylindrical cement implanted in tibiae of New Zealand white rabbits inoculated with S. aureus. We found that (1) in vitro, elution from VAN + US + MB cylinders was significantly higher than from either the VAN or VAN + US specimens; (2) the activity of the eluted VAN from the VAN + US + MB cylinders against planktonic S. aureus was significantly higher than from either the control or VAN or VAN + US specimens; and (3) in the rabbits, the activity of the eluted VAN from the VAN + US + MB cylinders against S. aureus was significantly higher than from either the control or VAN or VAN + US specimens. The present results suggest that VAN-loaded PMMA cement irradiated with MB-mediated US may have a role in controlling prosthetic joint infection.
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Tiao
authors: 0000-0002-7703-612X
authors: T
authors: Lin
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Xun-Zi
authors: 0000-0002-0595-9603
authors: XZ
authors: Cai
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Ming-Min
authors: MM
authors: Shi
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Zhi-Min
authors: ZM
authors: Ying
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Bin
authors: 0000-0003-3693-8204
authors: B
authors: Hu
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Chen-He
authors: CH
authors: Zhou
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Wei
authors: 0000-0002-6822-8162
authors: W
authors: Wang
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Zhong-Li
authors: ZL
authors: Shi
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors: Shi-Gui
authors: SG
authors: Yan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001843:Bone Cements / Q000494:pharmacology* / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D045423:Microbubbles; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D013977:Tibia / Q000000981:diagnostic imaging* / Q000187:drug effects; D014463:Ultrasonography; D014640:Vancomycin / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D001843:Bone Cements; D014640:Vancomycin; D019904:Polymethyl Methacrylate
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