title: Evaluation of BCL6 and MUM1 expression in patients with diffuse large B cell lymphoma and their correlations with staging and prognosis in Iran.
abstract: BACKGROUND
  Diffuse large B-cell lymphoma (DLBCL) is the most common form of non-Hodgkins lymphoma (NHL), accounting for approximately 25% of NHL cases. The aim of this study was to evaluate the association between the BCL6 and MUM1 gene expression and patient prognosis and stage.
  
  
  METHODS
  After ethical approval, in a cross-sectional study, tissue samples of 80 patients with diffuse large B-cell lymphoma were analyzed for BCL6 and MUM1 gene expression. Immunohistochemical staining was performed with division into categories of 0-5%, 5-25%, 26-50%, 51-75% and more than 75%. Other clinical and histological information such as lymph node involvement, T-stage, B symptoms and patient outcome were also recorded. Data were analyzed with SPSS version 16 and a P-value less than 0.05 was considered significant.
  
  
  RESULTS
  The patient mean age was 46.9±10.5 years (47.6±10.7 and 46.1±9.6 for males and females, respectively). A significant association was seen between lymphoma stage and BCL6 (p=0.045) but not MUM1 expression (p=0.09). However, the latter was associated with mortality (p=0.006) as was also the BCL6 level (p=0.006).
  
  
  CONCLUSIONS
  : Overexpression of MUM1 and BCL6 is associated with poor prognosis in patients with diffuse large B-cell lymphoma.
authors: Hematology Oncology Department, Mashhad University of Medical Science, Mashhad, Iran E-mail : rahmanish3@mums.ac.ir.
authors: Hossein
authors: H
authors: Rahimi
authors: Amirhossein
authors: A
authors: Jafarian
authors: Alireza
authors: A
authors: Samadi
authors: Bahram
authors: B
authors: Meamar
authors: Shaghayegh
authors: S
authors: Rahmani
mesh_terms: D003430:Cross-Sectional Studies; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006801:Humans; D050835:Interferon Regulatory Factors / Q000096:biosynthesis*; D007492:Iran; D016403:Lymphoma, Large B-Cell, Diffuse / Q000401:mortality* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000096:biosynthesis; D016335:Zinc Fingers / Q000235:genetics; D000099218:Interferon Regulatory Factor-4
chemical_list: D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors; D000099218:Interferon Regulatory Factor-4; C084103:BCL6 protein, human