title: Modulation of flavivirus population diversity by RNA interference.
abstract: To test the hypothesis that RNA interference (RNAi) imposes diversifying selection on RNA virus genomes, we quantified West Nile virus (WNV) quasispecies diversity after passage in Drosophila cells in which RNAi was left intact, depleted, or stimulated against WNV. As predicted, WNV diversity was significantly lower in RNAi-depleted cells and significantly greater in RNAi-stimulated cells relative to that in controls. These findings reveal that an innate immune defense can shape viral population structure.
authors: Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA doug.brackney@ct.gov.
authors: Doug E
authors: DE
authors: Brackney
authors: Department of Biology, New Mexico State University, Las Cruces, New Mexico, USA.
authors: Erin E
authors: EE
authors: Schirtzinger
authors: Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
authors: Thomas D
authors: TD
authors: Harrison
authors: Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
authors: Gregory D
authors: GD
authors: Ebel
authors: Department of Biology, New Mexico State University, Las Cruces, New Mexico, USA.
authors: Kathryn A
authors: KA
authors: Hanley
mesh_terms: D000818:Animals; D002460:Cell Line; D004330:Drosophila; D014644:Genetic Variation; D007113:Immunity, Innate; D034622:RNA Interference; D012641:Selection, Genetic; D014902:West Nile virus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*
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