title: Enzymatic production of glycerol acetate from glycerol.
abstract: In this study, we report the enzymatic production of glycerol acetate from glycerol and methyl acetate. Lipases are essential for the catalysis of this reaction. To find the optimum conditions for glycerol acetate production, sequential experiments were designed. Type of lipase, lipase concentration, molar ratio of reactants, reaction temperature and solvents were investigated for the optimum conversion of glycerol to glycerol acetate. As the result of lipase screening, Novozym 435 (Immobilized Candida antarctica lipase B) was turned out to be the optimal lipase for the reaction. Under the optimal conditions (2.5 g/L of Novozym 435, 1:40 molar ratio of glycerol to methyl acetate, 40 °C and tert-butanol as the solvent), glycerol acetate production was achieved in 95.00% conversion.
authors: Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul 139-701, Republic of Korea.
authors: Seokhyeon
authors: S
authors: Oh
authors: Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul 139-701, Republic of Korea. Electronic address: chpark@kw.ac.kr.
authors: Chulhwan
authors: C
authors: Park
mesh_terms: D000085:Acetates / Q000378:metabolism; D056804:Biofuels; D004075:Diglycerides / Q000096:biosynthesis; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D005656:Fungal Proteins / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D050178:Monoglycerides / Q000096:biosynthesis; D012997:Solvents; D013696:Temperature; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D000085:Acetates; D056804:Biofuels; D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides; D050178:Monoglycerides; D012997:Solvents; D014280:Triglycerides; C093667:Novozyme 435; D008049:Lipase; C431349:lipase B, Candida antarctica; D005990:Glycerol; C046923:methyl acetate
keywords: Glycerol; Glycerol acetate; Novozym 435; Optimization