title: Transplantation of bone marrow mononuclear cells modulates hippocampal expression of growth factors in chronically epileptic animals.
abstract: OBJECTIVE
  In previous studies, transplantation of bone marrow mononuclear cells (BMMCs) in epileptic animals has been found to be neuroprotective. However, the mechanism by which the BMMCs act remains unclear. We hypothesize that BMMCs may provide neuroprotection to the epileptic brain through trophic support. To test our hypothesis, we studied the temporal expression of neurotrophins after BMMC transplantation in the epileptic rat hippocampus.
  
  
  METHODS
  Chronically epileptic rats were intravenously transplanted with 1 × 10(7) BMMCs isolated from GFP transgenic mice. Expression levels of BDNF, GDNF, NGF, VEGF, and TGF-β1, and their receptors, were evaluated by ELISA and/or qRT-PCR analysis.
  
  
  RESULTS
  Our data revealed increased protein expression of BDNF, GDNF, NGF, and VEGF and reduced levels of TGF-β1 in the hippocampus of transplanted epileptic animals. Additionally, an increase in the mRNA expression of BDNF, GDNF, and VEGF, a reduction in TGF-β1, and a decrease in mRNA levels of the TrkA and TGFR-β1 receptors were also observed.
  
  
  CONCLUSIONS
  The gain provided by transplanted BMMCs in the epileptic brain may be related to the ability of these cells in modulating the network of neurotrophins and angiogenic signals.
authors: PUCRS, Pós-Graduação em Medicina e Ciências da Saúde, Instituto do Cérebro do Rio Grande do Sul (InsCer), Instituto de Pesquisas Biomódicas, Laboratório de Neurociências e Sinalização Celular, Porto Alegre, RS, Brazil.
authors: Gabriele
authors: G
authors: Zanirati
authors: Pamella Nunes
authors: PN
authors: Azevedo
authors: Daniel Rodrigo
authors: DR
authors: Marinowic
authors: Felipe
authors: F
authors: Rodrigues
authors: Ana Christina
authors: AC
authors: de Oliveira Dias
authors: Gianina Teribele
authors: GT
authors: Venturin
authors: Samuel
authors: S
authors: Greggio
authors: Fabrício
authors: F
authors: Simão
authors: Jaderson Costa
authors: JC
authors: DaCosta
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002908:Chronic Disease; D004195:Disease Models, Animal; D004827:Epilepsy / Q000378:metabolism* / Q000628:therapy*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010862:Pilocarpine; D017208:Rats, Wistar
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010862:Pilocarpine; D049452:Green Fluorescent Proteins
keywords: Epilepsy; Neurotrophic factors; Pilocarpine; Stem cells
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