title: A fluorescent Arg-Gly-Asp (RGD) peptide-naphthalenediimide (NDI) conjugate for imaging integrin α(v)β(3) in vitro.
abstract: We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.
authors: State Key Lab of Heavy Oil Processing, China University of Petroleum-Beijing, No. 18 Fuxue Road, 102249, Beijing, China. zh209@cup.edu.cn.
authors: Zhiyuan
authors: Z
authors: Hu
authors: Rory L
authors: RL
authors: Arrowsmith
authors: James A
authors: JA
authors: Tyson
authors: Vincenzo
authors: V
authors: Mirabello
authors: Haobo
authors: H
authors: Ge
authors: Ian M
authors: IM
authors: Eggleston
authors: Stanley W
authors: SW
authors: Botchway
authors: G Dan
authors: GD
authors: Pantos
authors: Sofia I
authors: SI
authors: Pascu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007094:Imides / Q000737:chemistry*; D039302:Integrin alphaVbeta3 / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D009281:Naphthalenes / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D007094:Imides; D039302:Integrin alphaVbeta3; D009281:Naphthalenes; D009842:Oligopeptides; C542131:naphthalenediimide; C047981:arginyl-glycyl-aspartic acid