title: Interleukin-1β-induced memory reconsolidation impairment is mediated by a reduction in glutamate release and zif268 expression and α-melanocyte-stimulating hormone prevented these effects.
abstract: The immune system is an important modulator of learning, memory and neural plasticity. Interleukin 1β (IL-1β), a pro-inflammatory cytokine, significantly affects several cognitive processes. Previous studies by our group have demonstrated that intrahippocampal administration of IL-1β impairs reconsolidation of contextual fear memory. This effect was reversed by the melanocortin alpha-melanocyte-stimulating hormone (α-MSH). The mechanisms underlying the effect of IL-1β on memory reconsolidation have not yet been established. Therefore, we examined the effect of IL-1β on glutamate release, ERK phosphorylation and the activation of the transcription factor zinc finger- 268 (zif268) during reconsolidation. Our results demonstrated that IL-1β induced a significant decrease of glutamate release after reactivation of the fear memory and this effect was related to calcium concentration in hippocampal synaptosomes. IL-1β also reduced ERK phosphorylation and zif268 expression in the hippocampus. Central administration of α-MSH prevented the decrease in glutamate release, ERK phosphorylation and zif268 expression induced by IL-1β. Our results establish possible mechanisms involved in the detrimental effect of IL-1β on memory reconsolidation and also indicate that α-MSH may exert a beneficial modulatory role in preventing IL-1β effects.
authors: IFEC-CONICET, Depto. Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
authors: Ivana
authors: I
authors: Machado
authors: IFEC-CONICET, Depto. Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
authors: Patricia V
authors: PV
authors: Gonzalez
authors: CIQUIBIC-CONICET, Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
authors: Alejandro
authors: A
authors: Vilcaes
authors: Instituto de Investigaciones Biomédicas INBIOMED UBA-CONICET, Facultad de Medicina, Buenos Aires, Argentina.
authors: Lila
authors: L
authors: Carniglia
authors: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors: Helgi B
authors: HB
authors: Schiöth
authors: Instituto de Investigaciones Biomédicas INBIOMED UBA-CONICET, Facultad de Medicina, Buenos Aires, Argentina.
authors: Mercedes
authors: M
authors: Lasaga
authors: IFEC-CONICET, Depto. Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. Electronic address: tscimonelli@mail.fcq.unc.edu.ar.
authors: Teresa N
authors: TN
authors: Scimonelli
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D005239:Fear / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism; D000521:alpha-MSH / Q000494:pharmacology*
chemical_list: D051766:Early Growth Response Protein 1; D053583:Interleukin-1beta; D018698:Glutamic Acid; D000521:alpha-MSH; D002118:Calcium
keywords: Alpha-melanocyte-stimulating hormone (α-MSH); Ca(2+); ERK; Glutamate release; Hippocampus; Interleukin-1β (IL-1β); Memory reconsolidation; zif268