title: [Use of a synthetic peptide of HIV-2 glycoprotein 36 in antigen mixtures for the immunodiagnosis of HIV types 1 and 2].
abstract: BACKGROUND
  The HIV-2 glycoprotein 36 (gp36) is often used in ELISA. An evaluation of the diagnostic indexes of antigen mixtures with a synthetic peptide of HIV2 gp36 (5) is performed in this study.
  
  
  METHODS
  Five mixtures of gp36 (5) and the recombinant proteins of HIV1/2 were prepared. A total of 1306 samples were evaluated with UMELISA HIV1+2 RECOMBINANT used as reference.
  
  
  RESULTS
  The variant (V-1) showed very good agreement as regards the reference method.
  
  
  CONCLUSIONS
  The V-1 variant was shown to be highly effective in the immunodiagnosis of HIV 1/2.
authors: Laboratorio de Retrovirus, Centro de Inmunoensayo, La Habana, Cuba.
authors: Grisell
authors: G
authors: Ramos Martínez
authors: Laboratorio de Retrovirus, Centro de Inmunoensayo, La Habana, Cuba. Electronic address: marcos.valdespino@cie.cu.
authors: Marcos Antonio
authors: MA
authors: Valdespino-Díaz
authors: Laboratorio de Síntesis de Péptidos, Centro de Inmunoensayo, La Habana, Cuba.
authors: Milenen
authors: M
authors: Hernández Marín
authors: Facultad de Biología, Universidad de la Habana, La Habana, Cuba.
authors: Elizabeth
authors: E
authors: Valle Carvajal
authors: Laboratorio de Retrovirus, Centro de Inmunoensayo, La Habana, Cuba.
authors: María Elena
authors: ME
authors: Selles León
authors: Laboratorio de Retrovirus, Centro de Inmunoensayo, La Habana, Cuba.
authors: Lilliam
authors: L
authors: Pozo Peña
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D015498:HIV-2 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000276:immunology; D012680:Sensitivity and Specificity; D054299:env Gene Products, Human Immunodeficiency Virus / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D010446:Peptide Fragments; D011994:Recombinant Proteins; D054299:env Gene Products, Human Immunodeficiency Virus; C059763:env protein gp36, Human immunodeficiency virus
keywords: ELISA; HIV; Péptido sintético; Synthetic peptide; VIH; gp36