title: Dysbiosis gut microbiota associated with inflammation and impaired mucosal immune function in intestine of humans with non-alcoholic fatty liver disease.
abstract: Non-alcoholic fatty liver disease (NAFLD) has recently been considered to be under the influence of the gut microbiota, which might exert toxic effects on the human host after intestinal absorption and delivery to the liver via the portal vein. In this study, the composition of the gut microbiota in NAFLD patients and healthy subjects was determined via 16S ribosomal RNA Illumina next-generation sequencing. Among those taxa displaying greater than 0.1% average abundance in all samples, five genera, including Alistipes and Prevotella, were significantly more abundant in the gut microbiota of healthy subjects compared to NAFLD patients. Alternatively, Escherichia, Anaerobacter, Lactobacillus and Streptococcus were increased in the gut microbiota of NAFLD patients compared to healthy subjects. In addition, decreased numbers of CD4+ and CD8+ T lymphocytes and increased levels of TNF-α, IL-6 and IFN-γ were detected in the NAFLD group compared to the healthy group. Furthermore, irregularly arranged microvilli and widened tight junctions were observed in the gut mucosa of the NAFLD patients via transmission electron microscopy. We postulate that aside from dysbiosis of the gut microbiota, gut microbiota-mediated inflammation of the intestinal mucosa and the related impairment in mucosal immune function play an important role in the pathogenesis of NAFLD.
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Weiwei
authors: W
authors: Jiang
authors: Institute of Clinical Molecular Biology &Central Laboratory, Peking University People's Hospital, Beijing, China.
authors: Na
authors: N
authors: Wu
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Xuemei
authors: X
authors: Wang
authors: Institute of Clinical Molecular Biology &Central Laboratory, Peking University People's Hospital, Beijing, China.
authors: Yujing
authors: Y
authors: Chi
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Yuanyuan
authors: Y
authors: Zhang
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Xinyun
authors: X
authors: Qiu
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Ying
authors: Y
authors: Hu
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Jing
authors: J
authors: Li
authors: Department of Gastroenterology, Peking University People's Hospital, Beijing, China.
authors: Yulan
authors: Y
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003430:Cross-Sectional Studies; D064806:Dysbiosis; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D008297:Male; D064307:Microbiota; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017423:Sequence Analysis, RNA; D019108:Tight Junctions / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D012336:RNA, Ribosomal, 16S; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
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