title: Transcriptomic analysis of Rongchang pig brains and livers.
abstract: Recent developments in high-throughput RNA sequencing (RNA-seq) technology have led to a dramatic impact on our understanding of the structure and expression profiles of the mammalian transcriptome. To gain insights into the usefulness of swine production and biomedical model, the transcriptome profiling of Rongchang pig brains and livers was characterized using RNA-seq technology to uncover functional candidate molecules. In the study, total RNAs from brains and livers of Rongchang pig were sequenced and 8.6Gb sequencing data was obtained. This analysis revealed tissue specificity through the identification of 5575 and 4600 differentially expressed genes (DEGs) in brains and livers, respectively and the functional analysis of DEGs. Furthermore, 83 neuropeptide gene transcripts, 69 neuropeptide receptor gene transcripts, 10 pro-neuropeptide convertase gene transcripts and many other neuropeptide related protein gene transcripts were identified. Totally, the major characteristics of the transcriptional profiles of Rongchang pig brains and livers were present.
authors: The Department of Veterinary Medicine, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China. Electronic address: gl9089@sina.com.
authors: Ling
authors: L
authors: Gan
authors: Center of Molecular Medicine, University of Georgia, Athens, GA 30602, USA. Electronic address: liweixie@uga.edu.
authors: Liwei
authors: L
authors: Xie
authors: The Department of Animal Husbandry, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China. Electronic address: zfuyuan@163.com.
authors: Fuyuan
authors: F
authors: Zuo
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. Electronic address: xbxzh@swu.edu.cn.
authors: Zhonghuai
authors: Z
authors: Xiang
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. Electronic address: hejia@swu.edu.cn.
authors: Ningjia
authors: N
authors: He
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017423:Sequence Analysis, RNA; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
keywords: Neuropeptide; Neuropeptide receptor; Pigs; RNA sequence; Transcriptome profiles