title: DNA-pairing and annealing processes in homologous recombination and homology-directed repair.
abstract: The formation of heteroduplex DNA is a central step in the exchange of DNA sequences via homologous recombination, and in the accurate repair of broken chromosomes via homology-directed repair pathways. In cells, heteroduplex DNA largely arises through the activities of recombination proteins that promote DNA-pairing and annealing reactions. Classes of proteins involved in pairing and annealing include RecA-family DNA-pairing proteins, single-stranded DNA (ssDNA)-binding proteins, recombination mediator proteins, annealing proteins, and nucleases. This review explores the properties of these pairing and annealing proteins, and highlights their roles in complex recombination processes including the double Holliday junction (DhJ) formation, synthesis-dependent strand annealing, and single-strand annealing pathways--DNA transactions that are critical both for genome stability in individual organisms and for the evolution of species.
authors: Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05405.
authors: Scott W
authors: SW
authors: Morrical
mesh_terms: D004247:DNA / Q000737:chemistry*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D045566:DNA, Cruciform / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology; D042822:Genomic Instability; D059765:Homologous Recombination; D008957:Models, Genetic; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*
chemical_list: D045566:DNA, Cruciform; D004268:DNA-Binding Proteins; D009692:Nucleic Acid Heteroduplexes; D004247:DNA
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