title: Effect of L-carnitine supplementation on maturation and early embryo development of immature mouse oocytes selected by brilliant cresyle blue staining.
abstract: OBJECTIVE
  The present study was designed to investigate the effect of L-carnitine treatment during IVM on nuclear and cytoplasmic maturation of immature oocytes selected by Brilliant Cresyle Blue (BCB) staining, and their subsequent developmental competence.
  
  
  METHODS
  Compact cumulus-oocyte complexes (COCs) were collected from NMRI mice ovaries and stained with BCB staining. BCB+ (colored cytoplasm) oocytes were then cultured in tissue culture medium (TCM) 199 with 0.0, 0.3 and 0.6 mg/ml L-carnitine.
  
  
  RESULTS
  The both L-carnitine concentrations significantly increased the intracellular glutathione (P<0.001), nuclear maturation (P<0.01) and expression levels of cyclin-dependent kinase1 (CDK1) (P<0.05). Moreover, treated oocytes with 0.6 mg/ml L-carnitine showed increased (P < 0.05) expression of mitogen-activated protein kinase1 (MAPK1) mRNA. Also, adding L-carnitine (0.6 mg/ml) to IVM medium significantly increased the cleavage rate (P<0.05). The blastocyst development rate (BDR) in the both L-carnitine treated groups was significantly higher (P<0.001) than the control group. L-carnitine had no significant effect on total blastocyst cell numbers.
  
  
  CONCLUSIONS
  These data indicated that L-carnitine supplementation during IVM of immature BCB+ oocytes improved preimplantation developmental competence of oocytes after IVF, probably by accelerating cytoplasmic and nuclear maturation of oocytes. It may provide a novel approach to improving ART outcomes in infertile couples.
authors: Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Zohreh
authors: Z
authors: Zare
authors: Reza
authors: R
authors: Masteri Farahani
authors: Mohammad
authors: M
authors: Salehi
authors: Abbas
authors: A
authors: Piryaei
authors: Marefat
authors: M
authors: Ghaffari Novin
authors: Fatemeh
authors: F
authors: Fadaei Fathabadi
authors: Moslem
authors: M
authors: Mohammadi
authors: Maryam
authors: M
authors: Dehghani-Mohammadabadi
mesh_terms: D000818:Animals; D001557:Benzenesulfonates; D002331:Carnitine / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D001557:Benzenesulfonates; C006796:brilliant blue; D002331:Carnitine
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