title: Induction of pro-inflammatory gene expression by Escherichia coli and mycotoxin zearalenone contamination and protection by a Lactobacillus mixture in porcine IPEC-1 cells.
abstract: This work investigated the effect of Escherichia coli K88 and zearalenone contamination on pro-inflammatory gene expression (Toll like receptors, cytokines) and signalling molecules and the protective activity of a mixture of Lactobacilli sp. (Lactobacillus plantarum, Lactobacillus acidofilus and Lactobacillus paracasei) in porcine intestinal epithelial cells as part of the local immune system. IPEC-1 cell monolayer was exposed for 1 h to the individual or combined action of E. coli, zearalenone and lactobacilli mixture. Our results showed that TLRs (1-10) and cytokine (IL-1,-6,-8,-10, TNF-α, IFN-γ) genes expressed early (after 1 h of culture) in IPEC-1 cells. E. coli alone increased the TLRs mRNA expression, especially TLR4 and the inflammatory cytokines while ZEA alone showed either no effect or a marginally effect on TLRs, cytokines, and signalling genes when compared to untreated cells. The combined actions of the two contaminants lead to a synergistically up-regulation of key cytokines (IFN-γ, IL-10 and TNF-α) and TLRs (-2,-3,-4,-6, and -10). The live lactobacilli mixture was able to attenuate the pathogen and mycotoxin-induced response by downregulated the majority of inflammatory related genes suggesting that this mixture has an immunomodulatory potential and may be used to lower the inflammatory response.
authors: Laboratory of Animal Biology, National Research and Development Institute for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania. Electronic address: ionelia.taranu@ibna.ro.
authors: Ionelia
authors: I
authors: Taranu
authors: Laboratory of Animal Biology, National Research and Development Institute for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors: Daniela Eliza
authors: DE
authors: Marin
authors: Laboratory of Animal Biology, National Research and Development Institute for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors: Gina Cecilia
authors: GC
authors: Pistol
authors: Laboratory of Animal Biology, National Research and Development Institute for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors: Monica
authors: M
authors: Motiu
authors: Department of Genetics, Biology Faculty, University of Bucharest, Intr. Portocalelor, No. 1-3, Sect. 6, Bucharest 060101, Romania.
authors: Diana
authors: D
authors: Pelinescu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D020895:Enterocytes / Q000187:drug effects*; D054307:Enterotoxigenic Escherichia coli / Q000472:pathogenicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D051193:Toll-Like Receptors / Q000378:metabolism; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D016207:Cytokines; D017931:DNA Primers; D012333:RNA, Messenger; D051193:Toll-Like Receptors; D015025:Zearalenone
keywords: Escherichia coli; Gene expressions; Intestin; Pig; Zearalenone