title: Age and metabolic risk factors associated with oxidatively damaged DNA in human peripheral blood mononuclear cells.
abstract: Aging is associated with oxidative stress-generated damage to DNA and this could be related to metabolic disturbances. This study investigated the association between levels of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs) and metabolic risk factors in 1,019 subjects, aged 18-93 years. DNA damage was analyzed as strand breaks by the comet assay and levels of formamidopyrimidine (FPG-) and human 8-oxoguanine DNA glycosylase 1 (hOGG1)-sensitive sites There was an association between age and levels of FPG-sensitive sites for women, but not for men. The same tendency was observed for the level of hOGG1-sensitive sites, whereas there was no association with the level of strand breaks. The effect of age on oxidatively damaged DNA in women disappeared in multivariate models, which showed robust positive associations between DNA damage and plasma levels of triglycerides, cholesterol and glycosylated hemoglobin (HbA1c). In the group of men, there were significant positive associations between alcohol intake, HbA1c and FPG-sensitive sites in multivariate analysis. The levels of metabolic risk factors were positively associated with age, yet only few subjects fulfilled all metabolic syndrome criteria. In summary, positive associations between age and levels of oxidatively damaged DNA appeared mediated by age-related increases in metabolic risk factors.
authors: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.
authors: Mille
authors: M
authors: Løhr
authors: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.
authors: Annie
authors: A
authors: Jensen
authors: National Institute of Public Health, University of Southern Denmark, Odense, Denmark.
authors: Louise
authors: L
authors: Eriksen
authors: National Institute of Public Health, University of Southern Denmark, Odense, Denmark.
authors: Morten
authors: M
authors: Grønbæk
authors: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.
authors: Steffen
authors: S
authors: Loft
authors: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.
authors: Peter
authors: P
authors: Møller
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood* / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D004249:DNA Damage; D045647:DNA Glycosylases / Q000378:metabolism; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008019:Life Style; D016014:Linear Models; D008055:Lipids / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D012307:Risk Factors; D012737:Sex Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D045647:DNA Glycosylases; D006442:Glycated Hemoglobin; D008055:Lipids; C517652:hemoglobin A1c protein, human; C473634:NEIL1 protein, human; C479058:oxoguanine glycosylase 1, human
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