title: Intestinal epithelial cells promote secretion of leptin and adiponectin in adipocytes.
abstract: Although leptin and adiponectin are the predominant adipokines, how their circulating levels are regulated is incompletely understood. The present study tested whether intestinal epithelial cells influence the expression and secretion of these adipokines by adipocytes. Leptin gene expression and secretion by cultured human primary adipocytes and Simpson-Golabi-Behmel Syndrome adipocytes increased upon coculture with human enterocytic Caco-2 cells or incubation in conditioned medium of Caco-2 cells. Although adiponectin secretion increased, its mRNA levels decreased. Tissue homogenate of the ileum (but not the jejunum, colon, or liver) of nonobese C57BL/6J mice also stimulated leptin and adiponectin secretion by cultured murine 3T3-L1 adipocytes. However, ileal homogenate of obese KK-Ay mice had no effect on leptin and adiponectin secretion. We propose that as yet unidentified humoral factors released from intestinal epithelial cells are involved in regulating circulating leptin and adiponectin levels. Decreased production of such factors may contribute to hyperphagia in KK-Ay mice.
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors: Rino
authors: R
authors: Ishihara
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors: Yuki
authors: Y
authors: Mizuno
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors: Akiho
authors: A
authors: Miwa
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors: Akihiro
authors: A
authors: Hamada
authors: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors: Takeshi
authors: T
authors: Tsuruta
authors: Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University of Ulm, Ulm 89075, Germany.
authors: Martin
authors: M
authors: Wabitsch
authors: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: ksnym@chem.agr.hokudai.ac.jp.
authors: Kei
authors: K
authors: Sonoyama
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D018938:Caco-2 Cells; D002450:Cell Communication / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D015854:Up-Regulation / Q000502:physiology
chemical_list: D052242:Adiponectin; D020738:Leptin
keywords: Adipocyte; Adiponectin; Intestine; Leptin