title: A portable and antibody-free sandwich assay for determination of chloramphenicol in food based on a personal glucose meter.
abstract: A portable and antibody-free sandwich assay was fabricated for determination of chloramphenicol (CAP) in animal-derived food by a personal glucose meter (PGM). The sandwich-type strategy was developed on the basis of magnetic molecularly imprinted probe (m-MIP) nanoparticles and a β-cyclodextrin/invertase-functionalized signal tag. Firstly, the m-MIPs were fabricated using polydopamine molecularly imprinted film modified Fe3O4 nanoparticles with 2,2-dichloroacetamide as a template that could capture the 2,2-dichloroacetamide segment of CAP. Secondly, β-cyclodextrin/invertase polymer bioconjungate was synthesized as a signal tag that could recognize the nitrobenzene segment of CAP through host-guest interaction. The dual-specificity recognition model relies on the formation of a sandwich between m-MIPs, different segments of CAP, and the β-cyclodextrin-functionalized signal tag. The sandwich-type complex formed was then subjected to detection with a PGM. The complexes can hydrolyze sucrose to glucose, which can be used for detection with a PGM through invertase. According to our experiment, the concentration of CAP was proportional to the amount of glucose formed, which could quantitatively assess the CAP with a dynamic range of 0.5-50 ng mL(-1) and a detection limit of 0.16 ng mL(-1) (signal-to-noise ratio of 3). The detection time of the assay was about 1 h, which was obviously shorter than that of competitive ELISA. More importantly, we have successfully applied this on-site assay for CAP screening in animal-derived food.
authors: Faculty of Marine Science, Ningbo University, Ningbo, 315211, China.
authors: Si
authors: S
authors: Chen
authors: Ning
authors: N
authors: Gan
authors: Huairong
authors: H
authors: Zhang
authors: Futao
authors: F
authors: Hu
authors: Tianhua
authors: T
authors: Li
authors: Huan
authors: H
authors: Cui
authors: Yuting
authors: Y
authors: Cao
authors: Qianli
authors: Q
authors: Jiang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002701:Chloramphenicol / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*
chemical_list: D002701:Chloramphenicol; D005947:Glucose