title: Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species.
abstract: Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78(T)) and the external surfaces of rice leaves (YE170(T)) collected in Thailand. The two strains were genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel species of the genus Yamadazyma although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit rRNA gene, the closest relative of strain ST-78(T) was Candida lessepsii CBS 9941(T) but with 3.8% nucleotide substitutions, while the closest relative of strain YE170(T) was strain ST-78(T) but with 4.3% nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1-2) regions revealed that strain ST-78 differed from C. lessepsii CBS 9941(T) by 8.8% nucleotide substitutions and from strain YE170(T) by 9.4% nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1-2 regions together with phylogenetic analysis indicated that strains ST-78(T) and YE170(T) represented two novel species within the Yamadazyma clade. The names Yamadazyma insecticola f.a., sp. nov. (type strain ST-78(T) = BCC 8314(T) = NBRC 110421(T) = CBS 13382(T); MycoBank no. MB810546) and Yamadazyma epiphylla f.a., sp. nov. (type strain YE170(T) = BCC 63466(T) = NBRC 110423(T) = CBS 13384(T); MycoBank no. MB810547) are proposed for the two novel yeast species.
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Sasitorn
authors: S
authors: Jindamorakot
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Somjit
authors: S
authors: Am-In
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Rungluk
authors: R
authors: Kaewwichian
authors: Center for Advanced Studies in Tropical Natural Resources, National Research University Kasetsart University, Bangkok 10900, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Savitree
authors: S
authors: Limtong
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005243:Feces / Q000382:microbiology; D007313:Insecta / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012275:Oryza; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal