title: Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection.
abstract: Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice and brutally affects the yield up to 50 % of total production. Here, we report a comparative proteomics analysis of total foliar protein isolated from infected rice leaves of susceptible Pusa Basmati 1 (PB1) and resistant Oryza longistaminata genotypes. Two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) approaches identified 29 protein spots encoding unique proteins from both the genotypes. Identified proteins belonged to a large number of biological and molecular functions related to biotic and abiotic stress proteins which are potentially involved during Xoo infection. Biotic and abiotic stress-related proteins were induced during Xoo infection, indicating the activation of common stress pathway during bacterial blight infection. Candidate genes conferring tolerance against bacterial blight, which include germin-like protein, putative r40c1, cyclin-dependent kinase C, Ent-isokaur-15-ene synthase and glutathione-dependent dehydroascorbate reductase 1 (GSH-DHAR1), were also induced, with germin-like proteins induced only in the resistant rice genotype O. longistaminata. Energy, metabolism and hypothetical proteins were common among both the genotypes. Further, host defence/stress-related proteins were mostly expressed in resistant genotype O. longistaminata, indicating possible co-evolution of the pathogen and the wild rice, O. longistaminata.
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad, 500046, India.
authors: Anirudh
authors: A
authors: Kumar
authors: Waikhom
authors: W
authors: Bimolata
authors: Monica
authors: M
authors: Kannan
authors: P B
authors: PB
authors: Kirti
authors: Insaf Ahmed
authors: IA
authors: Qureshi
authors: Irfan Ahmad
authors: IA
authors: Ghazi
mesh_terms: D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D060467:Disease Resistance / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D020543:Proteome; D002104:Cadmium; D005982:Glutathione Transferase; D016203:CDC2 Protein Kinase
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