title: Validity and reliability of a novel salivary immunoassay for individual profiling in applied sports science.
abstract: The aim of this study was to examine the validity and reliability of a novel immunoassay, developed to assess salivary Immunoglobulin A (s-IgA). Validity and reliability of the Individual Profiling Lateral Flow Device (IPRO LFD) for s-IgA concentrations ([s-IgA]) was assessed in males (n = 12) and females (n =13) who were involved in recreational activities. Reliability of the IPRO LFD method was assessed by comparing [s-IgA] of two saliva samples collected concurrently, while validity was assessed by comparing with an enzyme-linked immunosorbent assay (ELISA) method. The IPRO LFD had a strong positive correlation (r = 0.93, p < 0.001), with no difference in [s-IgA] compared with the ELISA. The IPRO LFD was considered reliable (ICC r = 0.89, p < 0.001 and CV = 9.40 %) for measures of [s-IgA]. We concluded that the IPRO LFD method may be a substitute to the ELISA method for measurements of [s-IgA].
authors: a Faculty of Health Sciences and Medicine , Bond University , Queensland , Australia.
authors: Sam
authors: S
authors: Coad
authors: Christopher
authors: C
authors: Mclellan
authors: Timothy
authors: T
authors: Whitehouse
authors: Bon
authors: B
authors: Gray
mesh_terms: D000328:Adult; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis*; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013178:Sports Medicine; D055815:Young Adult
chemical_list: D007070:Immunoglobulin A
keywords: athlete monitoring; bio-sensory technology; immunology