title: Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor.
abstract: Microencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. In this paper, we address the growth and Desmodus rotundus salivary plasminogen activator (DSPA) production of recombinant CHO (rCHO) in a stirred tank bioreactor. Cells were cultured using two different methods--in an unmicroencapsulated versus microencapsulated culture--and compared differences between them in terms of cell reproduction and DSPA protein productivity. Compared to the unmicroencapsulated rCHO, microencapsulated cells got higher cell density and prolonged the plateau phase. Microencapsulated rCHO promoted DSPA production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of DSPA was 3.3 higher than in unmicroencapsulated cells. Negative relationship was found between specific growth rate and DSPA production capacity of unit cells. These findings will facilitate the methods for higher DSPA production in stirred tank bioreactors.
authors: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China, wangy1802@dicp.ac.cn.
authors: Yu
authors: Y
authors: Wang
authors: Ying
authors: Y
authors: Zhang
authors: Na
authors: N
authors: Li
authors: Li
authors: L
authors: Chen
authors: Demeng
authors: D
authors: Zhang
authors: Dongsheng
authors: D
authors: Sun
authors: Guojun
authors: G
authors: Lv
authors: Weiting
authors: W
authors: Yu
authors: Xin
authors: X
authors: Guo
authors: Xiaojun
authors: X
authors: Ma
mesh_terms: D019149:Bioreactors; D004339:Drug Compounding; D010960:Plasminogen Activators / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; C069588:salivary plasminogen activator alpha 1, Desmodus rotundus; D010960:Plasminogen Activators