title: Osteoinductive activity of insulin-functionalized cell culture surfaces obtained using diazonium chemistry.
abstract: Polymeric surfaces suitable for cell culture (DR/Pec) were constructed from diazoresin (DR) and pectin (Pec) in a form of ultrathin films using the layer-by-layer (LbL) technique. The surfaces were functionalized with insulin using diazonium chemistry. Such functionalized surfaces were used to culture human mesenchymal stem cells (hMSCs) to assess their suitability for bone tissue engineering and regeneration. The activity of insulin immobilized on the surfaces (DR/Pec/Ins) was compared to that of insulin dissolved in the culture medium. Human MSC grown on insulin-immobilized DR/Pec surfaces displayed increased proliferation and higher osteogenic activity. The latter was determined by means of alkaline phosphatase (ALP) activity, which increases at early stages of osteoblasts differentiation. Insulin dissolved in the culture medium did not stimulate cell proliferation and its osteogenic activity was significantly lower. Addition of recombinant human bone morphogenetic protein 2 (rhBMP-2) to the culture medium further increased ALP activity in hMSCs indicating additive osteogenic action of immobilized insulin and rhBMP-2.
authors: Nanotechnology of Polymers and Biomaterials, Faculty of Chemistry, Jagiellonian University Kraków, Poland.
authors: Anna
authors: A
authors: Mikulska
authors: Department of Biology and Cell Imaging, Faculty of Biology and Earth Sciences, Jagiellonian University Kraków, Poland.
authors: Joanna
authors: J
authors: Filipowska
authors: Department of Biology and Cell Imaging, Faculty of Biology and Earth Sciences, Jagiellonian University Kraków, Poland.
authors: Anna M
authors: AM
authors: Osyczka
authors: Nanotechnology of Polymers and Biomaterials, Faculty of Chemistry, Jagiellonian University Kraków, Poland.
authors: Maria
authors: M
authors: Nowakowska
authors: Nanotechnology of Polymers and Biomaterials, Faculty of Chemistry, Jagiellonian University Kraków, Poland.
authors: Krzysztof
authors: K
authors: Szczubiałka
keywords: alkaline phosphatase; bone morphogenetic protein 2; cell culture surfaces; diazoresin; human mesenchymal stem cells; insulin; osteogenesis; pectin
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