title: Association of hsa-miR‑145 overexpression in human testicular cells with male infertility.
abstract: MicroRNAs (miRs) have crucial functions in spermatogenesis and implications for male infertility. In the present study, Homo sapiens (hsa)‑miR‑145 was designed and cloned into the eukaryotic expression plasmid pGenesil‑1. The recombinant plasmids were transfected into Hs 1.tes normal testicular cells and NTERA‑2 testicular cancer cells. Quantitative polymerase chain reaction of hsa‑miR‑145 indicated that pGenesil‑1‑miR‑145 effectively upregulated the expression of hsa‑miR‑145 in vitro. hsa‑miR‑145 overexpression inhibited the mRNA and protein expression of sex-determining region Y Box 9 in Hs 1.tes cells. The proliferation rates of NTERA‑2 cells transfected with pGenesil‑1‑miR‑145 were significantly decreased. High expression levels of miR‑145 promoted cell apoptosis in NTERA‑2 cells. The results revealed that altered hsa‑miR‑145 expression in testicular cells affects the regulation of target genes associated with male infertility.
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Liyuan
authors: L
authors: Zhang
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Xianping
authors: X
authors: Ding
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Shuangshuang
authors: S
authors: Nie
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Jesse
authors: J
authors: Li-Ling
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Yuping
authors: Y
authors: Zhang
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Hui
authors: H
authors: Zhang
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Lin
authors: L
authors: Chen
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Lingxiao
authors: L
authors: Li
authors: Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Min
authors: M
authors: Ding
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D030541:Databases, Genetic; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055758:SOX9 Transcription Factor / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; C502658:FSCN1 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; D050814:Octamer Transcription Factor-3; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D055748:SOXB1 Transcription Factors