title: Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon.
abstract: BACKGROUND
  A new Marseilleviridae virus family member, giant blood Marseille-like (GBM) virus, was recently reported in persons from France in the serum of an infant with adenitis, in the blood of 4% of healthy blood donors, and in 9% of multiply transfused thalassemia patients. These results suggested the presence of a nucleocytoplasmic large DNA virus potentially transmissible by blood product transfusion.
  
  
  METHODS
  To investigate this possibility we tested the plasma from 113 US blood donors and 74 multiply transfused Cameroon patients for GBM viral DNA using highly sensitive polymerase chain reaction (PCR) assays.
  
  
  RESULTS
  GBM DNA was not detected by nested PCR in any of these 187 human specimens.
  
  
  CONCLUSIONS
  Further testing is required to confirm the occurrence of human GBM virus infections.
authors: Blood Systems Research Institute.|Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California.
authors: Tung Gia
authors: TG
authors: Phan
authors: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France.
authors: Christelle
authors: C
authors: Desnues
authors: Laboratory Branch, Division of HIV/AIDS Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors: William M
authors: WM
authors: Switzer
authors: Global Viral Cameroon, Yaoundé, Cameroon.
authors: Cyrille F
authors: CF
authors: Djoko
authors: Metabiota, San Francisco, California.
authors: Bradley S
authors: BS
authors: Schneider
authors: Blood Systems Research Institute.|Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California.
authors: Xutao
authors: X
authors: Deng
authors: Blood Systems Research Institute.|Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California.
authors: Eric
authors: E
authors: Delwart
mesh_terms: D000048:Acanthamoeba / Q000821:virology; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors; D002163:Cameroon / Q000453:epidemiology; D017931:DNA Primers; D004266:DNA Virus Infections / Q000097:blood / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D004267:DNA Viruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood*; D005602:France / Q000453:epidemiology; D006801:Humans; D019169:Jurkat Cells / Q000821:virology; D008297:Male; D008875:Middle Aged; D016366:Open Reading Frames / Q000235:genetics; D010949:Plasma / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology; D065227:Transfusion Reaction; D014481:United States / Q000453:epidemiology; D014764:Viral Proteins / Q000097:blood / Q000235:genetics; D014766:Viremia / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins