title: Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons.
abstract: The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction time. Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions. These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.
authors: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.
authors: Bin
authors: B
authors: Liu
authors: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.
authors: Chunying
authors: C
authors: Deng
authors: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.
authors: Yuqin
authors: Y
authors: Zhang
authors: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.
authors: Jinxia
authors: J
authors: Zhang
keywords: Wnt3a; adipose-derived stem cells; cholinergic neurons; differentiation; induction; neural regeneration; neural stem cells
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