title: Sodium metabisulphite, a preservative agent, decreases the heart capillary volume and length, and curcumin, the main component of Curcuma longa, cannot protect it.
abstract: Sodium metabisulphite is used as an antioxidant agent in many pharmaceutical formulations. It is extensively used as a food preservative and disinfectant. It has been demonstrated that sulphite exposure can affect some organs. Curcumin, the main element of Curcuma longa, has been identified to have multiple protective properties. The present study extends the earlier works to quantitative evaluation of the effects of sulphite and curcumin on the heart structure using stereological methods. In this study, 28 rats were randomly divided into four experimental groups. The rats in groups I to IV received distilled water (group I), sodium metabisulphite (25 mg/ kg/day) (group II), curcumin (100 mg/kg/day) (group III), and sodium metabisulphite+curcumin (group IV), respectively, for 8 weeks. The left ventricle was subjected to stereological methods to estimate the quantitative parameters of the myocardium. A 20 % decrease was observed in the total volume of ventricular tissue in the sulphite-treated animals compared to the distilled water treatment (P < 0.02). Also, the volume and length of the capillaries were reduced by 43 % on average in the sulphite-treated rats in comparison to the distilled water-treated animals (P < 0.02). However, no significant change was seen in the mean and total volume of the myocardium and the cavity and diameter of the capillaries after sulphite ingestion. Treatment with curcumin did not protect the animals against the structural changes of the ventricle. Sulphite, as a preservative food agent, reduced the length and volume of the ventricular capillaries and curcumin could not protect them.
authors: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: A
authors: A
authors: Noorafshan
authors: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: R
authors: R
authors: Asadi-Golshan
authors: Department of Basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: S
authors: S
authors: Monjezi
authors: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: S
authors: S
authors: Karbalay-Doust
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects* / Q000648:ultrastructure; D048429:Cell Size; D003238:Connective Tissue / Q000187:drug effects / Q000648:ultrastructure; D003326:Coronary Circulation / Q000187:drug effects; D003331:Coronary Vessels / Q000187:drug effects* / Q000648:ultrastructure; D003474:Curcumin / Q000494:pharmacology*; D005520:Food Preservatives / Q000633:toxicity*; D006352:Heart Ventricles / Q000187:drug effects*; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009206:Myocardium / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000648:ultrastructure; D009929:Organ Size; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013447:Sulfites / Q000633:toxicity*
chemical_list: D005520:Food Preservatives; D013447:Sulfites; C005200:sodium metabisulfite; D003474:Curcumin