title: Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients.
abstract: A novel electrochemical sensing method was devised for the first time to detect plasma cortisol, a potential psychological stress biomarker, in human immunodeficiency virus (HIV)-positive subjects. A miniaturized potentiostat (reconfigured LMP91000 chip) interfaced with a microfluidic manifold containing a cortisol immunosensor was employed to demonstrate electrochemical cortisol sensing. This fully integrated and optimized electrochemical sensing device exhibited a wide cortisol-detection range from 10 pg/mL to 500 ng/mL, a low detection limit of 10 pg/mL, and sensitivity of 5.8 μA (pg mL)(-1), with a regression coefficient of 0.995. This cortisol-selective sensing system was employed to estimate plasma cortisol in ten samples from HIV patients. The electrochemical cortisol-sensing performance was validated using an enzyme-linked immunosorbent assay technique. The results obtained using both methodologies were comparable within 2%-5% variation. The information related to psychological stress of HIV patients can be correlated with disease-progression parameters to optimize diagnosis, therapeutic, and personalized health monitoring.
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Ajeet
authors: A
authors: Kaushik
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Adriana
authors: A
authors: Yndart
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Rahul Dev
authors: RD
authors: Jayant
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Vidya
authors: V
authors: Sagar
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Venkata
authors: V
authors: Atluri
authors: BioMEMS Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA.
authors: Shekhar
authors: S
authors: Bhansali
authors: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immun ology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, USA.
authors: Madhavan
authors: M
authors: Nair
mesh_terms: D015415:Biomarkers / Q000097:blood; D055664:Electrochemical Techniques / Q000379:methods*; D015658:HIV Infections / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D019095:Point-of-Care Systems; D013315:Stress, Psychological
chemical_list: D015415:Biomarkers; D006854:Hydrocortisone
keywords: HIV; cortisol; electrochemical immunosensing; miniaturized sensing device; personalized health care; psychological stress
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