title: In situ surface-enhanced Raman spectroelectrochemical analysis system with a hemin modified nanostructured gold surface.
abstract: An integrated surface-enhanced Raman scattering (SERS) spectroelectrochemical (SEC) analysis system is presented that combines a small volume microfluidic sample chamber (<100 μL) with a compact three-electrode configuration for in situ surface-enhanced Raman spectroelectrochemistry. The SEC system includes a nanostructured Au surface that serves dual roles as the electrochemical working electrode (WE) and SERS substrate, a microfabricated Pt counter electrode (CE), and an external Ag/AgCl reference electrode (RE). The nanostructured Au WE enables highly sensitive in situ SERS spectroscopy through large and reproducible SERS enhancements, which eliminates the need for resonant wavelength matching of the laser excitation source with the electronic absorption of the target molecule. The new SEC analysis system has the merits of wide applicability to target molecules, small sample volume, and a low detection limit. We demonstrate in situ SERS spectroelectrochemistry measurements of the metalloporphyrin hemin showing shifts of the iron oxidation marker band ν4 with the nanostructured Au working electrode under precise potential control.
authors: Analytical Biochemistry and Department of Pharmacy, University of Groningen , Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
authors: Tao
authors: T
authors: Yuan
authors: Loan
authors: L
authors: Le Thi Ngoc
authors: Jan
authors: J
authors: van Nieuwkasteele
authors: Mathieu
authors: M
authors: Odijk
authors: Albert
authors: A
authors: van den Berg
authors: Hjalmar
authors: H
authors: Permentier
authors: Rainer
authors: R
authors: Bischoff
authors: Edwin T
authors: ET
authors: Carlen
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties
chemical_list: D006427:Hemin; D006046:Gold