title: Study on the mechanism of the interaction between acteoside and pepsin using spectroscopic techniques.
abstract: The interaction of acteoside with pepsin has been investigated using fluorescence spectra, UV/vis absorption spectra, three-dimensional (3D) fluorescence spectra and synchronous fluorescence spectra, along with a molecular docking method. The fluorescence experiments indicate that acteoside can quench the intrinsic fluorescence of pepsin through combined quenching at a low concentration of acteoside, and static quenching at high concentrations. Thermodynamic analysis suggests that hydrogen bonds and van der Waal's forces are the main forces between pepsin and acteoside. According to the theory of Förster's non-radiation energy transfer, the binding distance between pepsin and acteoside was calculated to be 2.018 nm, which implies that energy transfer occurs between acteoside and pepsin. In addition, experimental results from UV/vis absorption spectra, 3D fluorescence spectra and synchronous fluorescence spectra imply that pepsin undergoes a conformation change when it interacts with acteoside.
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Yifeng
authors: Y
authors: Fang
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Hong
authors: H
authors: Xu
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Liangliang
authors: L
authors: Shen
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Fengwen
authors: F
authors: Huang
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Shadaiti
authors: S
authors: Yibulayin
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Songyang
authors: S
authors: Huang
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Shengli
authors: S
authors: Tian
authors: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, China.
authors: Zhangli
authors: Z
authors: Hu
authors: School of Medicine, Shenzhen University, Shenzhen, 518060, China.
authors: Zhendan
authors: Z
authors: He
authors: Shenzhen Entry-Exit inspection and Quarantine Bureau, Shenzhen, 518001, China.
authors: Fangrong
authors: F
authors: Li
authors: Shenzhen Entry-Exit inspection and Quarantine Bureau, Shenzhen, 518001, China.
authors: Yinong
authors: Y
authors: Li
authors: Shenzhen Marine Environment and Resource Monitoring Center, Shenzhen, 518060, China.
authors: Kai
authors: K
authors: Zhou
mesh_terms: D001665:Binding Sites; D004735:Energy Transfer; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005960:Glucosides / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D062105:Molecular Docking Simulation; D010434:Pepsin A / Q000737:chemistry* / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010434:Pepsin A; D010636:Phenols; C058956:acteoside; D059808:Polyphenols
keywords: acteoside; interaction; molecular docking; pepsin; spectroscopy