title: [Effects of Mycobacterium tuberculosis antigen and anti-TCRγδ antibody on IL-17 production of human γδT cells in polarization culture].
abstract: OBJECTIVE
  To investigate the effects of Mycobacterium tuberculosis heat-resistant antigen (MTB-HAg) and anti-TCR γδ monoclonal antibody (mAb) on the induction and differentiation of human IL-17-producing γδT cells under polarization culture conditions.
  
  
  METHODS
  From human peripheral blood mononuclear cells, the γδT cells were purified by magnetic-activated cell sorting (MACS) and then stimulated with MTB-HAg or anti-TCRγδ mAb and with or without anti-CD28 mAb, and cultured in the presence or absence of the cytokine cocktails (CK) (IL-1β, IL-6, TGF-β and IL-23) for 10 to 12 days. The polarized cultured γδT cells were collected and re-stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 6 hours, and the number of IL-17-producing cells was detected by ELISPOT.
  
  
  RESULTS
  The number of IL-17-producing cells (per 10⁵ γδT cells) in anti-TCRγδ combined with CK group was significantly higher than that in the anti-TCRγδ group. Meanwhile, the IL-17-producing γδT cell number in anti-TCRγδ combined with anti-CD28 group was lower than that in the anti-TCRγδ combined with anti-CD28 and CK group. The IL-17-producing γδT cell number of anti-TCRγδ combined with anti-CD28 and CK group was significantly higher than that in anti-TCRγδ combined with CK group, and that in MTB-HAg combined with anti-CD28 and CK group was significantly lower than that in anti-TCRγδ combined with anti-CD28 and CK group, but higher than that in MTB-HAg combined with anti-CD28 group.
  
  
  CONCLUSIONS
  The CK (IL-1β, IL-6, TGF-β and IL-23) required for the differentiation of Th17 cells also induced the differentiation of IL-17⁺ γδT cells after pre-activated by MTB-HAg or anti-TCRγδ antibody, while anti-TCRγδ mAb showed the stronger stimulatory effect than MTB-HAg. In addition, CD28 co-stimulation enhanced the differentiation of IL-17 from activated γδT cells.
authors: Department of Microbiology and Parasitology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Li
authors: L
authors: Chen
authors: Department of Microbiology and Parasitology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Hui
authors: H
authors: Xia
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Hongtao
authors: H
authors: Wang
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Lingling
authors: L
authors: Sheng
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Zhiqing
authors: Z
authors: Xu
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Yao
authors: Y
authors: Sun
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Jie
authors: J
authors: Tang
authors: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
authors: Baiqing
authors: B
authors: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology*; D053759:Interleukin-23 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D020381:Interleukin-17; D053759:Interleukin-23; D015850:Interleukin-6; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016212:Transforming Growth Factor beta; C072681:Mycobacterium tuberculosis antigens