title: Inhalation toxicology methods: the generation and characterization of exposure atmospheres and inhalational exposures.
abstract: In this unit, the need for laboratory-based inhalation toxicology studies, the historical background on adverse health effects of airborne toxicants, and the benefits of advance planning for the building of analytic options into the study design to maximize the scientific gains to be derived from the investments in the study are outlined. The following methods are described: (1) the generation and characterization of exposure atmospheres for inhalation exposures in humans and laboratory animals; (2) the delivery and distribution into and within whole-body exposure chambers, head-only exposure chambers, face-masks, and mouthpieces or nasal catheters; (3) options for on-line functional assays during and between exposures; and (4) options for serial non-invasive assays of response. In doing so, a description beyond exposures to single agents and simple mixtures is presented, and included are methods for evaluating biological responses to complex environmental mixtures. It is also emphasized that great care should be taken in the design and execution of such studies so that the scientific returns can be maximized both initially, and in follow-up utilization of archived samples of the exposure atmospheres, excreta, and tissues collected for histology.
authors: Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, New York.
authors: Lung-Chi
authors: LC
authors: Chen
authors: Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, New York.
authors: Morton
authors: M
authors: Lippmann
mesh_terms: D000336:Aerosols; D000818:Animals; D001273:Atmosphere Exposure Chambers; D004785:Environmental Pollutants / Q000009:adverse effects* / Q000378:metabolism; D004867:Equipment Design; D005260:Female; D006801:Humans; D019570:Inhalation Exposure; D008297:Male; D051379:Mice; D023421:Models, Animal; D010316:Particle Size; D051381:Rats; D015203:Reproducibility of Results; D012137:Respiratory System / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D018570:Risk Assessment; D013686:Telemetry; D014018:Tissue Distribution; D014116:Toxicology / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000336:Aerosols; D004785:Environmental Pollutants
keywords: aerosols; airborne toxicants; animal exposures; atmosphere generation; complex mixtures; exposure chambers; exposure-response; extrapulmonary; human exposures; inhalation exposures; non-invasive; particle size; telemetry
references: Arch Environ Health. 1969 Jan;18(1):30-41
references: 5782495
references: Am J Respir Crit Care Med. 2007 Jan 15;175(2):126-35
references: 17095746
references: Inhal Toxicol. 2014 Aug;26(10):588-97
references: 25144474
references: Inhal Toxicol. 2005 Apr;17(4-5):235-41
references: 15804941
references: Environ Sci Technol. 2007 Oct 15;41(20):6969-75
references: 17993136
references: Inhal Toxicol. 2011 Feb;23(2):95-103
references: 21261442
references: Toxicol Appl Pharmacol. 1987 Jun 15;89(1):1-8
references: 3590183
references: J Toxicol Environ Health A. 2008;71(20):1352-62
references: 18704828
references: Am J Physiol Heart Circ Physiol. 2006 Sep;291(3):H985-1002
references: 16632549
references: Environ Health Perspect. 2011 Feb;119(2):176-81
references: 20864429
references: J Toxicol Environ Health. 1990;29(2):169-84
references: 2299692
references: Toxicol Sci. 2006 Jan;89(1):135-44
references: 16207940
references: Am Ind Hyg Assoc J. 1969 May-Jun;30(3):257-75
references: 5793995
references: Inhal Toxicol. 2011 Aug;23 Suppl 2:1-10
references: 21639692
references: Public Health Monogr. 1959;57:1-54
references: 13824625
references: Toxicol Appl Pharmacol. 1983 Oct;71(1):123-31
references: 6636178
references: Herz. 2007 Oct;32(7):568-72
references: 17972030
references: Environ Sci Technol. 1986 Feb 1;20(2):138-45
references: 22288800
references: Mech Ageing Dev. 2001 Apr 15;122(4):415-26
references: 11240163
references: Inhal Toxicol. 2010 Jun;22(7):580-92
references: 20387995
references: JAMA. 2005 Dec 21;294(23):3003-10
references: 16414948
references: J Expo Sci Environ Epidemiol. 2014 Jan-Feb;24(1):105-12
references: 24220216
references: Inhal Toxicol. 2014 Sep;26(11):643-50
references: 25162718
references: Inhal Toxicol. 2010 Jan;22(1):33-41
references: 20017592
references: Ann Otol Rhinol Laryngol. 1970 Jun;79(3):519-28
references: 5426884
references: J Immunotoxicol. 2009 Dec;6(4):266-75
references: 19908945
references: Diabetes. 2009 Feb;58(2):344-51
references: 19008341
references: J Vasc Res. 2007;44(2):138-48
references: 17215585
references: Mol Cancer Ther. 2006 May;5(5):1117-25
references: 16731743
references: Fundam Appl Toxicol. 1981 Jan-Feb;1(1):8-12
references: 7184776
references: PLoS One. 2012;7(4):e35114
references: 22509389
references: Toxicol Sci. 2006 Nov;94(1):183-92
references: 16929010
references: Crit Rev Toxicol. 2014 Sep;44(8):643-95
references: 25168068
references: J Toxicol Environ Health. 1992 Mar;35(3):165-74
references: 1602522
references: Arch Environ Health. 1969 May;18(5):738-55
references: 5781920
references: Am J Respir Cell Mol Biol. 2010 Jan;42(1):96-104
references: 19346316
references: Inhal Toxicol. 2010 Dec;22 Suppl 2:95-9
references: 20936915
references: Arch Environ Health. 1970 Jul;21(1):25-31
references: 5422321
references: Neurotoxicology. 2013 May;36:112-7
references: 22057156
references: Am Ind Hyg Assoc J. 1981 Apr;42(4):273-82
references: 7234686
references: Inhal Toxicol. 2005 Apr;17(4-5):243-53
references: 15804942
references: Inhal Toxicol. 2005 Apr;17(4-5):189-97
references: 15804936
references: Am Ind Hyg Assoc J. 1992 Aug;53(8):503-9
references: 1509990
references: Environ Health Perspect. 2006 Nov;114(11):1662-9
references: 17107850
references: Toxicol Sci. 2002 Jan;65(1):115-25
references: 11752691
references: Arch Environ Health. 1975 Jul;30(7):361-7
references: 1147701
references: Am Ind Hyg Assoc J. 1983 Dec;44(12):923-8
references: 6660193
references: Exp Lung Res. 2006 May;32(5):181-99
references: 16908446
references: Inhal Toxicol. 2010 Aug;22(9):738-53
references: 20462391
references: Nanotoxicology. 2010 Mar 1;4(1):106-119
references: 20730025
references: Inhal Toxicol. 2010 May;22(6):449-59
references: 20235771
references: J Toxicol Environ Health. 1984;13(2-3):441-69
references: 6376822
references: Am Ind Hyg Assoc J. 1980 Jun;41(6):399-409
references: 7395753
references: Res Rep Health Eff Inst. 2013 Oct;(177):5-13
references: 24377209
references: J Immunol. 2005 Sep 15;175(6):3577-83
references: 16148101
references: Res Rep Health Eff Inst. 2013 Oct;(178):5-8
references: 24377210
references: Am Ind Hyg Assoc J. 1984 May;45(5):285-92
references: 6741776
references: Inhal Toxicol. 2010 Dec;22 Suppl 2:100-6
references: 21142798
references: Toxicol Appl Pharmacol. 1997 Jun;144(2):348-55
references: 9194419
references: J Toxicol Environ Health A. 2002 Oct 25;65(20):1531-43
references: 12396867
references: Curr Opin Endocrinol Diabetes Obes. 2007 Oct;14(5):365-9
references: 17940464
references: Environ Health Perspect. 1989 Feb;79:147-50
references: 2707194
references: Res Rep Health Eff Inst. 2000 Aug;(96):5-74; disc. 75-86
references: 11205815
references: Environ Health Perspect. 2003 Jun;111(7):981-91
references: 12782502
references: Cell Prolif. 2008 Feb;41 Suppl 1:146-64
references: 18181954
references: Free Radic Res. 2000 Nov;33 Suppl:S99-108
references: 11191280
references: Environ Health Perspect. 1989 Feb;79:167-72
references: 2707196
references: Inhal Toxicol. 2011 Aug;23 Suppl 2:95-103
references: 21913822