title: Observational scores of dampness and mold associated with measurements of microbial agents and moisture in three public schools.
abstract: We examined associations between observational dampness scores and measurements of microbial agents and moisture in three public schools. A dampness score was created for each room from 4-point-scale scores (0-3) of water damage, water stains, visible mold, moldy odor, and wetness for each of 8 room components (ceiling, walls, windows, floor, ventilation, furniture, floor trench, and pipes), when present. We created mixed microbial exposure indices (MMEIs) for each of 121 rooms by summing decile ranks of 8 analytes (total culturable fungi; total, Gram-negative, and Gram-positive culturable bacteria; ergosterol; (1→3)-β-D-glucan; muramic acid; and endotoxin) in floor dust. We found significant (P ≤ 0.01) linear associations between the dampness score and culturable bacteria (total, Gram-positive, and Gram-negative) and the MMEIs. Rooms with dampness scores greater than 0.25 (median) had significantly (P < 0.05) higher levels of most microbial agents, MMEIs, and relative moisture content than those with lower scores (≤0.25). Rooms with reported recent water leaks had significantly (P < 0.05) higher dampness scores than those with historical or no reported water leaks. This study suggests that observational assessment of dampness and mold using a standardized form may be valuable for identifying and documenting water damage and associated microbial contamination.
authors: Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV, USA.
authors: S J
authors: SJ
authors: Cho
authors: Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV, USA.
authors: J M
authors: JM
authors: Cox-Ganser
authors: Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV, USA.
authors: J-H
authors: JH
authors: Park
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000706:statistics & numerical data*; D001419:Bacteria; D004391:Dust; D004731:Endotoxins / Q000032:analysis; D004784:Environmental Monitoring; D004875:Ergosterol / Q000032:analysis; D005658:Fungi / Q000254:growth & development*; D006813:Humidity; D012574:Schools / Q000706:statistics & numerical data*; D014691:Ventilation
chemical_list: D004391:Dust; D004731:Endotoxins; D004875:Ergosterol
keywords: Assessment; Building; Dampness; Microbial; Mold; School
references: J Environ Monit. 2011 Apr;13(4):1082-7
references: 21359295
references: Indoor Air. 2011 Apr;21(2):156-64
references: 21204984
references: Sci Total Environ. 2012 Jul 15;430:75-81
references: 22634552
references: Occup Environ Med. 2013 May;70(5):325-31
references: 23396522
references: Occup Environ Med. 2013 Oct;70(10):681-7
references: 23775866
references: Indoor Air. 2014 Apr;24(2):158-70
references: 24016225
references: Indoor Air. 2001 Jun;11(2):72-86
references: 11394014
references: Indoor Air. 2001 Sep;11(3):192-9
references: 11521504
references: Environ Health Perspect. 2002 May;110(5):543-7
references: 12003761
references: Appl Environ Microbiol. 2003 Jun;69(6):3103-9
references: 12788704
references: Indoor Air. 2004 Aug;14(4):243-57
references: 15217478
references: Indoor Air. 2004 Dec;14(6):425-33
references: 15500636
references: Environ Res. 1992 Apr;57(2):212-30
references: 1568442
references: Indoor Air. 1999 Mar;9(1):26-32
references: 10195273
references: Indoor Air. 2006 Jun;16(3):192-203
references: 16683938
references: Scand J Public Health. 2007;35(2):125-32
references: 17454915
references: Sci Total Environ. 2007 Nov 15;387(1-3):276-82
references: 17692898
references: Indoor Air. 2009 Aug;19(4):280-90
references: 19500175
references: J Microbiol Methods. 2010 Jul;82(1):78-84
references: 20434494
references: Comput Methods Programs Biomed. 2011 Apr;102(1):75-80
references: 21146248
references: Environ Health Perspect. 2011 Jun;119(6):748-56
references: 21269928