title: Synthesis of triazole-nucleoside phosphoramidites and their use in solid-phase oligonucleotide synthesis.
abstract: Triazole-backbone oligonucleotides are macromolecules that have one or more triazole units that are acting as a backbone mimic. Triazoles within the backbone have been used within oligonucleotides for a variety of applications. This unit describes the preparation and synthesis of two triazole-nucleoside phosphoramidites [uracil-triazole-uracil (UtU) and cytosine-triazole-uracil (CtU)] based on a PNA-like scaffold, and their incorporation within oligonucleotides.
authors: Faculty of Science, University of Ontario Institute of Technology, Oshawa, Ontario, Canada.
authors: Brandon J
authors: BJ
authors: Peel
authors: Tim C
authors: TC
authors: Efthymiou
authors: Jean-Paul
authors: JP
authors: Desaulniers
mesh_terms: D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides; D014230:Triazoles
keywords: cycloaddition; phosphoramidite; solid‐phase oligonucleotide synthesis; triazole‐linkage