title: Immune response against 2,4-dinitrofluorobenzene-induced atopic dermatitis-like clinical manifestation is suppressed by spermidine in NC/Nga mice.
abstract: Of the biogenic polyamines, spermidine is a natural constituent of living cells and organisms. Spermidine is associated with regulation of cell growth, proliferation and differentiation, and with the suppression of oxidation and inflammation. Atopic dermatitis (AD) is a chronic inflammatory skin disease that has a complex and multiple pathogenesis, which includes genetic abnormality, modified or abnormal immune response and the production of nitric oxide and reactive oxygen species. We investigated whether spermidine can relieve AD-like clinical manifestation induced by the continual application of 2,4-dinitrofluorobenzene (DNFB) in NC/Nga mice. Spermidine at concentrations of 1 or 10 mg/kg reduced increasing ear swelling and attenuated oedema, haemorrhage and hyperkeratosis in AD-like skin lesions. Repetitive application of DNFB induced inflammatory cell infiltration to skin lesions, whereas intraperitoneal injection of spermidine inhibited DNFB-evoked infiltration of eosinophils, mast cells and T lymphocytes. Furthermore, spermidine suppressed mast cell degranulation and production of interferon-gamma by activated CD4(+) T cells in AD-like skin lesions. Spermidine may be a potential therapeutic agent for treatment of AD.
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul, Korea.
authors: G-D
authors: GD
authors: Kim
authors: T-H
authors: TH
authors: Kim
authors: Y S
authors: YS
authors: Park
authors: H-J
authors: HJ
authors: Ahn
authors: J-J
authors: JJ
authors: Cho
authors: C-S
authors: CS
authors: Park
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000008:administration & dosage*; D006651:Histocytochemistry; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D051379:Mice; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013095:Spermidine / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D013095:Spermidine