title: Distribution of miRNA genes in the pig genome.
abstract: BACKGROUND
  Recent completion of swine genome may simplify the production of swine as a large biomedical model. Here we studied sequence and location of known swine miRNA genes, key regulators of protein-coding genes at the level of RNA, and compared them to human and mouse data to prioritize future molecular studies.
  
  
  RESULTS
  Distribution of miRNA genes in pig genome shows no particular relation to different genomic features including protein coding genes - proportions of miRNA genes in intergenic regions, introns and exons roughly agree with the size of these regions in the pig genome. Our analyses indicate that host genes harbouring intragenic miRNAs are longer from other protein-coding genes, however, no important GO enrichment was found. Swine mature miRNAs show high sequence similarity to their human and mouse orthologues. Location of miRNA genes relative to protein-coding genes is also similar among studied species, however, there are differences in the precise position in particular intergenic regions and within particular hosts. The most prominent difference between pig and human miRNAs is a large group of pig-specific sequences (53% of swine miRNAs). We found no evidence that this group of evolutionary new pig miRNAs is different from old miRNAs genes with respect to genomic location except that they are less likely to be clustered.
  
  
  CONCLUSIONS
  There are differences in precise location of orthologues miRNA genes in particular intergenic regions and within particular hosts, and their meaning for coexpression with protein-coding genes deserves experimental studies. Functional studies of a large group of pig-specific sequences in future may reveal limits of the pig as a model organism to study human gene expression.
authors: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poland, Wolynska 33, 60-637, Poznan, Poland. paulina.paczynska@gmail.com.
authors: Paulina
authors: P
authors: Paczynska
authors: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poland, Wolynska 33, 60-637, Poznan, Poland. adrian.grzemski@gmail.com.
authors: Adrian
authors: A
authors: Grzemski
authors: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poland, Wolynska 33, 60-637, Poznan, Poland. maciej@up.poznan.pl.
authors: Maciej
authors: M
authors: Szydlowski
mesh_terms: D000818:Animals; D016678:Genome; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010802:Phylogeny; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D035683:MicroRNAs
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