title: Regulation of autophagy of prostate cancer cells by β-catenin signaling.
abstract: OBJECTIVE
  Autophagy is a cellular degradation process for the recycling of damaged or superfluous intracellular compartments to provide an alternative energy source during periods of metabolic stress for maintaining cell homeostasis and viability. Although autophagy in different contexts have been shown to use similar signaling pathways, the exact molecular regulation of autophagy has been found to be cell-type dependent.
  
  
  METHODS
  We used rapamycin to trigger autophagy and used nitric oxide (NO) to inhibit autophagy in prostate cancer cells. IWP-2 was used to inhibit β-catenin signaling. Autophagy-associated proteins were examined by Western blot.
  
  
  RESULTS
  We found that nitric oxide (NO), a potent cellular messenger, impaired rapamycin-induced autophagy in prostate cancer cells. Further analyses showed that NO induced nuclear accumulation of β-catenin, a key factor of Wnt signaling pathway, to inhibit autophagy in prostate cancer cells.
  
  
  CONCLUSIONS
  We demonstrate involvement of β-catenin signaling in the regulation of autophagy of prostate cancer cells. Our results shed light on a previously unappreciated β-catenin signaling pathway for regulating autophagy in prostate cancer.
authors: Department of Urology Surgery, No. 180 Hospital of PLA, Quanzhou, China.
authors: Rongkai
authors: R
authors: Lin
authors: Jiayu
authors: J
authors: Feng
authors: Shaoliang
authors: S
authors: Dong
authors: Runyang
authors: R
authors: Pan
authors: Huiqiang
authors: H
authors: Zhuang
authors: Zhenyu
authors: Z
authors: Ding
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000187:drug effects / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D020123:Sirolimus / Q000008:administration & dosage; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D051176:beta Catenin; D009569:Nitric Oxide; D020123:Sirolimus