title: [Effect of Bushen Tongdu Capsule on RANK/RANKL/OPG pathway of collagen induced arthritis rats].
abstract: OBJECTIVE
  To study the effect of Bushen Tongdu Capsule (BTC) on RANK/RANKL/ OPG pathway of collagen induced arthritis (CIA) rats, thereby laying theoretic evidence for treating rheumatic arthritis (RA) by Chinese medicine.
  
  
  METHODS
  RA model was induced by CIA. Totally 42 rats were randomly divided into six groups, i.e., the normal control group, the model group, the low dose BTC (BSL) group, the medium dose BTC (BSM) group, the high dose BTC (BSH) group, and the Tripterygium Glycosides (TG) group, 7 in each group. BTC at the daily dose of 120, 240, and 480 mg/kg was given by gastrogavage to rats in the BSL, BSM, and BSH group respectively from the 13th day of modeling. TG at the daily dose of 24 mg/kg was given by gastrogavage to rats in the TG group. All medication was given once daily, 2 mL each time. Two mL normal saline was administered to rats in the normal control group and the model group. All medication lasted for 18 days. Samples were taken at day 31. The TRAP section of the ankle joint was fixed in 10% formalin for TRAP stain. Serum levels of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), and macrophage colony-stimulating factor (M-CSF) were detected using ELISA.
  
  
  RESULTS
  Compared with the normal control group, positive reactions of pathological ankle joint section, inflammation, and osteoclasia degree were significantly improved in the model group, serum levels of RANKL and M-CSF were up-regulated, levels of OPG and OPG/RANKL were significantly lowered (all P < 0.01). Compared with the model group, positive reactions of pathological ankle joint section, inflammation, and osteoclasia degree also significantly decreased in the BSH group and the TG group (all P < 0.01). RANKL and M-CSF were significantly down-regulated in each medicated group, while levels of OPG and OPG/RANKL were significantly up-regulated (all P < 0.01). Compared with the TG group, M-CSF was lower, but levels of OPG and OPG/RANKL were significantly up-regulated in the normal control group (all P < 0.01). RANKL and M-CSF were significantly up-regulated, while levels of OPG and OPG/RANKL were significantly down-regulated in the model group and each BS group (all P < 0.01).
  
  
  CONCLUSIONS
  BTC could relieve bone damage of CIA rats possibly through regulating and controlling osteoclasts.
authors: Yang-Chun
authors: YC
authors: Zhu
authors: Lin
authors: L
authors: Lin
authors: Xiao-Li
authors: XL
authors: Zhou
authors: Rong-Fang
authors: RF
authors: Li
authors: Li-Ping
authors: LP
authors: Hou
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007249:Inflammation; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D010010:Osteoclasts; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism; D029757:Tripterygium
chemical_list: D004365:Drugs, Chinese Herbal; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D016173:Macrophage Colony-Stimulating Factor