title: Evaluation of metronidazole-loaded poly(3-hydroxybutyrate) membranes to potential application in periodontitis treatment.
abstract: Guided tissue regeneration is a technique used for periodontium reconstruction. This technique uses barrier membranes, which prevent epithelial growth in the wound site and may also be used to release antibiotics, to protect the wound against opportunistic infections. Periodontal poly(3-hydroxybutyrate) membranes containing metronidazole (a drug used to help in infection control) were produced and characterized. The kinetic mechanism of the metronidazole delivery of leached and nonleached membrane as well as its cytotoxicity and structural integrity were evaluated. Poly(3-hydroxybutyrate) membranes containing 0.5-2 wt % of the drug and 20 wt % of the plasticizer were manufactured via compression molding. Based on morphological analysis, membranes loaded with 2% metronidazole were considered for detailed studies. The results revealed that metronidazole delivery by the leached membranes seemed to follow the Fick's law. Membranes were noncytotoxic. The amount of metronidazole delivered was in the range of the minimal inhibitory concentration for Porphyromonas gingivalis, and the membranes inhibited the proliferation of these bacteria. Besides, they maintained their mechanical resistance after 30 days of immersion in phosphate buffer at pH 7.4.
authors: COPPE/Program of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Marcio A C
authors: MA
authors: da Silva
authors: COPPE/Program of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Renata N
authors: RN
authors: Oliveira
authors: Chemical Engineering Department of the Technology Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Roberta Helena
authors: RH
authors: Mendonça
authors: Institute of Microbiology Paulo de Góes, Centre of Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Talita G B
authors: TG
authors: Lourenço
authors: Institute of Microbiology Paulo de Góes, Centre of Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Ana Paula V
authors: AP
authors: Colombo
authors: Bioceramics and Cell Culture Lab, Brazilian Center for Physics Research, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Marcelo N
authors: MN
authors: Tanaka
authors: Bioceramics and Cell Culture Lab, Brazilian Center for Physics Research, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Elena M O
authors: EM
authors: Tude
authors: COPPE/Program of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Marysilvia F
authors: MF
authors: da Costa
authors: COPPE/Program of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
authors: Rossana Mara S M
authors: RM
authors: Thiré
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics; D004353:Drug Evaluation, Preclinical; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008567:Membranes, Artificial; D008795:Metronidazole / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy*; D011091:Polyesters / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000254:growth & development*; D000094122:Polyhydroxybutyrates
chemical_list: D000900:Anti-Bacterial Agents; D006885:Hydroxybutyrates; D008567:Membranes, Artificial; D008795:Metronidazole; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: antimicrobial; drug delivery/release; guided tissue regeneration (GTR); periodontitis; poly(3-hydroxybutyrate)