title: [Experimental study of adipose-derived mesenchymal stem cells in the treatment of Crohn's disease].
abstract: OBJECTIVE
  To explore the efficacy of adipose-derived mesenchymal stem cells (ADMSCs) in a murine model of inflammatory bowel disease, and its potential mechanism.
  
  
  METHODS
  Murine colitis mouse model of Crohn's disease(CD) was created by trinitrobenzene sulfonic acid(TNBS)-induced colitis. Seventy-five 6-8 weeks female BALB/c mice were randomly divided into 3 groups: control group, TNBS group and ADMSC group. To verify the therapeutic effect of ADMSC, real-time PCR and immunohistochemical staining were performed to measure inflammatory cytokines levels in colon tissues. The 10-day survival statuses were recorded after the infusion of ADMSCs.
  
  
  RESULTS
  Intraperitoneal injection of ADMSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and increased survival(60% vs. 30%, P<0.05) in the TNBS-induced mouse model of CD. Compared with TNBS group, proinflammatory cytokines, including TNF-α, IL-12 and VEGF of ADMSC group were significantly reduced, with significant increase of IL-10 expression.
  
  
  CONCLUSIONS
  ADMSCs can effectively repair the injury of colonitis through down-regulation of proinflammatory cytokines TNF-α, IL-12 and VEGF expression, and up-regulation of anti-inflammatory cytokine IL-10 expression, which may be a potential new alternative of cell-based therapy for CD.
authors: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, China. sabiston@126.com.
authors: Minghao
authors: M
authors: Xie
authors: Xiaosheng
authors: X
authors: He
authors: Jinling
authors: J
authors: Zhu
authors: Zhen
authors: Z
authors: He
authors: Xiaowen
authors: X
authors: He
authors: Ping
authors: P
authors: Lan
authors: Lei
authors: L
authors: Lian
mesh_terms: D017667:Adipocytes; D000818:Animals; D003092:Colitis; D003424:Crohn Disease; D016207:Cytokines; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D015212:Inflammatory Bowel Diseases; D059630:Mesenchymal Stem Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D014302:Trinitrobenzenesulfonic Acid; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D014302:Trinitrobenzenesulfonic Acid