title: Hippocampal receptor complexes paralleling LTP reinforcement in the spatial memory holeboard test in the rat.
abstract: The current study was designed to examine learning-induced transformation of early-LTP into late-LTP. Recording electrodes were implanted into the dentate gyrus of the hippocampus in male rats and early-LTP was induced by weak tetanic stimulation of the medial perforant path. Dorsal right hippocampi were removed, membrane proteins were extracted, separated by blue-native gel electrophoresis with subsequent immunoblotting using brain receptor antibodies. Spatial training resulted into reinforcement of LTP and the reinforced LTP was persistent for 6h. Receptor complex levels containing GluN1 and GluN2A of NMDARs, GluA1 and GluA2 of AMPARs, nAchα7R and the D(1A) dopamine receptor were significantly-elevated in rat hippocampi of animals underwent spatial learning, whilst levels of GluA3 and 5-HT1A receptor containing complexes were significantly reduced. Evidence for complex formation between GluN1 and D(1A) dopamine receptor was provided by antibody shift assay, co-immunoprecipitation and mass spectrometric analysis. Thus our results propose that behavioural stimuli like spatial learning reinforce early LTP into late LTP and this reinforced LTP is accompanied by changes in certain receptor levels in the membrane fraction of the rat hippocampus.
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien.
authors: Saraswathi
authors: S
authors: Subramaniyan
authors: Institute of Biology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany.
authors: Vahid
authors: V
authors: Hajali
authors: Institute of Biology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany.
authors: Thomas
authors: T
authors: Scherf
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien.
authors: Sunetra Jitkar
authors: SJ
authors: Sase
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien.
authors: Fernando J
authors: FJ
authors: Sialana
authors: Core facilities, Core Facility Imaging, Medizinische Universität Wien, Anna Spiegel Forschungsgebäude, 1090 Vienna, Austria.
authors: Marion
authors: M
authors: Gröger
authors: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT25.3 and 1090 Vienna, Austria.
authors: Keiryn L
authors: KL
authors: Bennett
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien.
authors: Arnold
authors: A
authors: Pollak
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien.
authors: Lin
authors: L
authors: Li
authors: Institute of Biology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany. Electronic address: volker.korz@ovgu.de.
authors: Volker
authors: V
authors: Korz
authors: Department of Paediatrics, Medizinische Universität Wien, Währinger Gürtel 18A, 1090 Wien. Electronic address: gert.lubec@meduniwien.ac.at.
authors: Gert
authors: G
authors: Lubec
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000502:physiology*; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D019580:Perforant Pathway / Q000502:physiology*; D017208:Rats, Wistar; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D018091:Receptors, AMPA / Q000378:metabolism; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012054:Reinforcement, Psychology; D065853:Spatial Learning / Q000502:physiology; D065852:Spatial Memory / Q000502:physiology*; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000378:metabolism
chemical_list: D044282:Receptor, Serotonin, 5-HT1A; D018091:Receptors, AMPA; D017447:Receptors, Dopamine D1; D016194:Receptors, N-Methyl-D-Aspartate; D064569:alpha7 Nicotinic Acetylcholine Receptor; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2
keywords: Early-LTP; Hippocampus; LTP reinforcement; Late-LTP; Membrane receptor; Spatial learning