title: The oncolytic effects of reovirus in canine solid tumor cell lines.
abstract: Oncolytic virotherapy is a new strategy for cancer treatment for humans and dogs. Reovirus has been proven to be a potent oncolytic virus in human medicine. Our laboratory has previously reported that canine mast cell tumor and canine lymphoma were susceptible to reovirus. In this study, canine solid tumor cell lines (mammary gland tumor, osteosarcoma and malignant melanoma) were tested to determine their susceptibility towards reovirus. We demonstrated that reovirus induces more than 50% cell death in three canine mammary gland tumors and one canine malignant melanoma cell line. The reovirus-induced cell death occurred via the activation of caspase 3. Ras activation has been shown to be one of the important mechanisms of reovirus-susceptibility in human cancers. However, Ras activation was not related to the reovirus-susceptibility in canine solid tumor cell lines, which was similar to reports in canine mast cell tumor and canine lymphoma. The results of this study highly suggest that canine mammary gland tumor and canine malignant melanoma are also potential candidates for reovirus therapy in veterinary oncology.
authors: Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors: Masaya
authors: M
authors: Igase
authors: Chung Chew
authors: CC
authors: Hwang
authors: Matt
authors: M
authors: Coffey
authors: Masaru
authors: M
authors: Okuda
authors: Shunsuke
authors: S
authors: Noguchi
authors: Takuya
authors: T
authors: Mizuno
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000821:virology*; D008545:Melanoma / Q000821:virology*; D050504:Oncolytic Viruses / Q000502:physiology*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D012087:Reoviridae / Q000502:physiology*; D014779:Virus Replication
chemical_list: D011065:Poly(ADP-ribose) Polymerases
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