title: Surface modification of titanium substrate with a novel covalently-bound copolymer thin film for improving its platelet compatibility.
abstract: Despite of its widely uses in various clinical applications, the titanium-based material still faces different challenges, such as hemocompatibility and anti-biofouling characteristics required in various situations. The objective of this investigation was to develop a novel surface modification strategy for titanium-based material to improve the platelet compatibility that is important in rigorous blood-contacting cardiovascular applications. In this work, a series of copolymers, which composed of novel 6-acryloyloxy hexyl phosphonic acid (AcrHPA) and sulfobetaine methacrylate (SBMA) was synthesized. The phosphonic acid group in these copolymers can impart covalent binding to the titanium substrate while the zwitterionic sulfobetaine functionality is considered being able to reduce the platelet adhesion and activation on the modified titanium substrate. NMR analyses suggested that copolymerization reaction is likely not an ideal statistical reaction but to add the monomers in a random order. Studies have shown that the composition of the monomers affected the surface characteristics and platelet compatibility of these covalent-bound AcrHPA-SBMA copolymers on titanium substrate. Contact angle analysis has shown the addition of SBMA can increase surface hydrophilicity of the spun-coated copolymers. In addition, AFM analyses have revealed that the surface roughness of the spun-coated copolymer layer were varied with the ratio of AcrHPA and SBMA. The most platelet compatible surface was noted on the one modified by the highest amount of SBMA added (i.e. 70 mol%) in copolymerization. In summary, the surface modification scheme presented here would be of potential as well as manufacturing process applicable for future development in blood-contacting titanium-based biomedical devices.
authors: Department of Chemical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan.
authors: Ching-Hsiung
authors: CH
authors: Shen
authors: Yu-Jen
authors: YJ
authors: Cho
authors: Yi-Ching
authors: YC
authors: Lin
authors: Li-Chin
authors: LC
authors: Chien
authors: Tzer-Min
authors: TM
authors: Lee
authors: Wen-Hsi
authors: WH
authors: Chuang
authors: Jui-Che
authors: JC
authors: Lin
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D010757:Phosphorous Acids / Q000737:chemistry / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects* / Q000502:physiology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D010757:Phosphorous Acids; C521589:sulfobetaine methacrylate polymer; C570063:phosphonic acid; D014025:Titanium
references: J Mech Behav Biomed Mater. 2013 Apr;20:407-15
references: 23507261
references: Langmuir. 2012 Jan 10;28(1):640-7
references: 22111508
references: J Biomed Mater Res. 1994 Feb;28(2):217-24
references: 8207034
references: Thromb Haemost. 1999 Jul;82(1):58-64
references: 10456455
references: J Biomed Mater Res A. 2008 Feb;84(2):425-35
references: 17618480
references: Langmuir. 2010 Feb 2;26(3):1747-54
references: 20039608
references: Biomacromolecules. 2006 Sep;7(9):2552-9
references: 16961317
references: J Periodontal Implant Sci. 2011 Dec;41(6):263-72
references: 22324003
references: Langmuir. 2009 Dec 1;25(23):13516-21
references: 19689148
references: Macromol Biosci. 2011 Nov 10;11(11):1515-25
references: 21818855
references: Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):215-20
references: 21807481
references: Langmuir. 2004 Mar 16;20(6):2270-3
references: 15835682
references: Biomacromolecules. 2006 Apr;7(4):1329-34
references: 16602757
references: Int J Artif Organs. 1993 Jul;16(7):545-50
references: 8370610
references: Acta Biomater. 2012 Nov;8(11):3888-903
references: 22765961
references: Colloids Surf B Biointerfaces. 2004 Nov 25;39(1-2):87-94
references: 15542345
references: Adv Mater. 2010 Mar 5;22(9):920-32
references: 20217815
references: J Biomed Mater Res A. 2013 Nov;101(11):3349-64
references: 23766134
references: Langmuir. 2011 Jun 7;27(11):7091-8
references: 21563809
references: Biomaterials. 2008 Nov;29(32):4285-91
references: 18722010
references: Langmuir. 2005 Jan 4;21(1):225-8
references: 15620307
references: Langmuir. 2010 Jan 5;26(1):74-82
references: 19691273
references: Langmuir. 2006 Sep 12;22(19):8197-204
references: 16952262
references: J Biomed Mater Res A. 2009 Oct;91(1):18-28
references: 18683221
references: Biomed Eng Online. 2007 Jul 17;6:31
references: 17640335
references: J Biomed Mater Res. 2000 Nov;52(2):413-21
references: 10951383
references: Colloids Surf B Biointerfaces. 2013 Jan 1;101:376-83
references: 23010044
references: Langmuir. 2008 May 20;24(10):5453-8
references: 18399670
references: J Biomed Mater Res A. 2009 Sep 15;90(4):947-58
references: 18646200
references: J Phys Chem B. 2006 Dec 28;110(51):25603-5
references: 17181194
references: J Biomed Mater Res A. 2010 Nov;95(2):341-9
references: 20623672