title: Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy.
abstract: Multiphoton microscopy has become a novel biological imaging technique that allows cellular and subcellular microstructure imaging based on two-photon excited fluorescence and second harmonic generation. In this work, we used multiphoton microscopy to obtain the high-contrast images of human normal breast tissue and two main histologic types of fibroadenoma (intracanalicular, pericanalicular). Moreover, quantitative image analysis was performed to characterize the changes of collagen morphology (collagen content, collagen orientation). The results show that multiphoton microscopy combined with quantitative method has the ability to identify the characteristics of fibroadenoma including changes of the duct architecture and collagen morphology in stroma. With the advancement of multiphoton microscopy, we believe that the technique has great potential to be a real-time histopathological diagnostic tool for intraoperative detection of fibroadenoma in the future.
authors: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.
authors: Y T
authors: YT
authors: Nie
authors: Y
authors: Y
authors: Wu
authors: F M
authors: FM
authors: Fu
authors: Y E
authors: YE
authors: Lian
authors: S M
authors: SM
authors: Zhuo
authors: C
authors: C
authors: Wang
authors: J X
authors: JX
authors: Chen
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000648:ultrastructure*; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure; D005260:Female; D018226:Fibroadenoma / Q000175:diagnosis / Q000648:ultrastructure*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055815:Young Adult
chemical_list: D003094:Collagen
keywords: Fibroadenoma; multiphoton microscopy; normal breast tissue