title: Rapid mitochondrial DNA isolation method for direct sequencing.
abstract: Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.
authors: Department of Genetics, Albert Einstein College of Medicine, New York, NY, 10461, USA.
authors: Wilber
authors: W
authors: Quispe-Tintaya
authors: Ryan R
authors: RR
authors: White
authors: Vasily N
authors: VN
authors: Popov
authors: Jan
authors: J
authors: Vijg
authors: Alexander Y
authors: AY
authors: Maslov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans
chemical_list: D004272:DNA, Mitochondrial