title: A molecular signature for the prediction of recurrence in colorectal cancer.
abstract: BACKGROUND
  Several clinical and pathological factors have an impact on the prognosis of colorectal cancer (CRC), but they are not yet adequate for risk assessment. We aimed to identify a molecular signature that can reliably identify CRC patients at high risk for recurrence.
  
  
  RESULTS
  Two hundred eighty-one CRC samples (stage II/III) were included in this study. A two-step gene expression profiling study was conducted. First, gene expression measurements from 81 fresh frozen CRC samples were obtained using Affymetrix Human Genome U133 Plus 2.0 Arrays. Second, a focused gene expression assay, including prognostic genes and genes of interest from literature reviews, was performed using 200 fresh frozen samples and a Taqman low-density array (TLDA) analysis. An optimal 31-gene expression classifier for the prediction of recurrence among patients with stage II/III CRC was developed using logistic regression analysis. This gene expression signature classified 58.5% of patients as low-risk and 41.5% as high-risk (P < 0.001). The signature was the strongest independent prognostic factor in the multivariate analysis. The five-year relapse-free survival (RFS) rates for the low-risk patients and the high-risk patients were 88.5% and 41.3% (P < 0.001), respectively.
  
  
  CONCLUSIONS
  We identified a 31-gene expression signature that is closely associated with the clinical outcome of stage II/III CRC patients.
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. lishawang9@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. lishawang9@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. lishawang9@163.com.
authors: Lisha
authors: L
authors: Wang
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. 21326217@qq.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. 21326217@qq.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. 21326217@qq.com.
authors: Xiaohan
authors: X
authors: Shen
authors: Department of Genetics, Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center and Shanghai Industrial Technology Institute, Shanghai, 201203, China. wangzhm@chgc.sh.cn.
authors: Zhimin
authors: Z
authors: Wang
authors: Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, China. xiaoxiuying2002@163.com.
authors: Xiuying
authors: X
authors: Xiao
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. ripplepz@gmail.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. ripplepz@gmail.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. ripplepz@gmail.com.
authors: Ping
authors: P
authors: Wei
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. wangqifeng19821982@126.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. wangqifeng19821982@126.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. wangqifeng19821982@126.com.
authors: Qifeng
authors: Q
authors: Wang
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. renfei0319@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. renfei0319@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. renfei0319@163.com.
authors: Fei
authors: F
authors: Ren
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. yiqinwang11@fudan.edu.cn.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. yiqinwang11@fudan.edu.cn.|Institute of Pathology, Fudan University, Shanghai, 200032, China. yiqinwang11@fudan.edu.cn.
authors: Yiqin
authors: Y
authors: Wang
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. zebing080@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. zebing080@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. zebing080@163.com.
authors: Zebing
authors: Z
authors: Liu
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. shengweiqi2006@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. shengweiqi2006@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. shengweiqi2006@163.com.
authors: Weiqi
authors: W
authors: Sheng
authors: Department of Genetics, Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center and Shanghai Industrial Technology Institute, Shanghai, 201203, China. huangwei@chgc.sh.cn.
authors: Wei
authors: W
authors: Huang
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. 13524324387@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. 13524324387@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. 13524324387@163.com.
authors: Xiaoyan
authors: X
authors: Zhou
authors: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China. dx2008cn@163.com.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. dx2008cn@163.com.|Institute of Pathology, Fudan University, Shanghai, 200032, China. dx2008cn@163.com.|Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China. dx2008cn@163.com.
authors: Xiang
authors: X
authors: Du
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging / Q000379:methods; D011379:Prognosis; D011446:Prospective Studies; D012306:Risk; D059467:Transcriptome / Q000235:genetics*
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