title: DHEA Alleviates Oxidative Stress of Muscle Cells via Activation of Nrf2 Pathway.
abstract: Dehydroepiandrosterone (DHEA) has been proposed to regulate muscle dystrophy, while the underlying mechanisms for its protection against muscle atrophy are unknown. The present study was carried out to improve our understanding of the mechanism of DHEA's protective effect on muscle cells. We observed that DHEA significantly decreased the loss of cell death associated with H2O2-induced toxicity. Pretreating the muscle cells with DHEA led to a reduction of the intracellular accumulation of reactive oxygen species (ROS) in response to H2O2. In addition, DHEA reduced the H2O2-induced phosphorylation of ERK and p38 in a dose-dependent manner. Moreover, DHEA stimulated the activation of Nrf2, which led to the expression of an antioxidant response gene, HO-1. These results suggest that both antioxidants and anti-inflammatory properties mediate DHEA's effects for protection against muscle atrophy.
authors: Dongguk University Research Institute of Biotechnology, Seoul, 100-715, Republic of Korea.
authors: Songhee
authors: S
authors: Jeon
authors: Jinyoung
authors: J
authors: Hur
authors: Jongpil
authors: J
authors: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009133:Muscular Atrophy / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D032446:Myoblasts / Q000378:metabolism* / Q000473:pathology; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D009124:Muscle Proteins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D003687:Dehydroepiandrosterone; D006861:Hydrogen Peroxide; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D048051:p38 Mitogen-Activated Protein Kinases