title: Identification and characterization of MAVS from black carp Mylopharyngodon piceus.
abstract: MAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate immune response against microbial pathogens. In this study, MAVS has been cloned and characterized from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to mammalian MAVS. bcMAVS is constitutively transcribed in all the selected tissues including gill, kidney, heart, intestine, liver, muscle, skin and spleen; bcMAVS mRNA level in intestine, liver, muscle increased but decreased in spleen right after GCRV or SVCV infection. Multiple bands of bcMAVS were detected in western blot when it was expressed in tissue culture, which is similar to mammalian MAVS. Immunofluorescence assay determined that bcMAVS is a mitochondria protein and luciferase reporter assay demonstrated that bcMAVS could induce zebrafish IFN and EPC IFN expression in tissue culture. Data generated in this manuscript has built a solid foundation for further elucidating the function of bcMAVS in the innate immune system of black carp.
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Wei
authors: W
authors: Zhou
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Jujun
authors: J
authors: Zhou
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Ying
authors: Y
authors: Lv
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Yixiao
authors: Y
authors: Qu
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Mengdie
authors: M
authors: Chi
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China.
authors: Jun
authors: J
authors: Li
authors: Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan, 430072, China. Electronic address: fenghao@hunnu.edu.cn.
authors: Hao
authors: H
authors: Feng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012087:Reoviridae / Q000502:physiology; D012088:Reoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary / Q000821:virology; D012209:Rhabdoviridae / Q000502:physiology; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary / Q000821:virology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029941:Fish Proteins
keywords: Black carp; Innate immunity; MAVS