title: Association of eNOS gene polymorphisms and systemic lupus erythematosus in southeast Iran.
abstract: OBJECTIVE
  Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with unknown etiology. Genetic and environmental factors play important roles in the pathogenesis of SLE. The primary objective of this study was to investigate the possible association of eNOS gene intron 4b/a, Glu298Asp and T-786C polymorphisms with SLE in southeast Iran populations.
  
  
  METHODS
  This was a case-control study comparing eNOS polymorphisms in 106 SLE patients and 196 age- and sex-matched healthy controls. The 4b/a, Glu298Asp and T-786C polymorphisms were analyzed using polymerase chain reaction and restriction fragment length polymorphism.
  
  
  RESULTS
  Our findings indicated that the 4b/a polymorphism was associated with SLE, and the risk of SLE was 3.5- and 1.75-fold higher in patients with aa and ba genotypes than in patients with bb genotype. No association was observed between Glu298Asp and T-786C polymorphisms and SLE. There were no differences in eNOS gene polymorphisms between the Balouch and Fars population.
  
  
  CONCLUSIONS
  Statistically significant differences were observed in genotypes and allele frequencies of 4b/a polymorphism between patients with SLE and healthy controls in southeast Iran.
authors: Department of Internal Medicine, Ali-EbneAbitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.|Clinical Research Development Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Mahnaz
authors: M
authors: Sandoughi
authors: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.|Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Saeedeh
authors: S
authors: Salimi
authors: Department of Internal Medicine, Ali-EbneAbitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.|Clinical Research Development Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Zahra
authors: Z
authors: Zakeri
authors: Department of Internal Medicine, Ali-EbneAbitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Ebrahim Jahani
authors: EJ
authors: Darbandi
authors: Department of Pathology, Ali-EbneAbitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Mehdi
authors: M
authors: Jahantigh
authors: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Bita
authors: B
authors: Moudi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005091:Exons; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns; D007492:Iran; D008180:Lupus Erythematosus, Systemic / Q000000981:diagnostic imaging / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D012307:Risk Factors; D055815:Young Adult
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: endothelial nitric oxide synthase; polymorphism; systemic lupus erythematosus