title: Long circulating micelles of an amphiphilic random copolymer bearing cell outer membrane phosphorylcholine zwitterions.
abstract: Polymeric micelles with cell outer membrane mimetic structure were prepared in water from amphiphilic random copolymers bearing both the hydrophilic phosphorylcholine zwitterions and hydrophobic octadecyl side chains of cell outer membrane. The polymeric micelles showed sizes ranging from 80 nm to 120 nm in hydrodynamic diameter and zeta-potentials from -6.4 mV to -2.4 mV by dynamic light scattering measurements. The micelles loaded with 6-coumarin as a fluorescence probe were stable to investigate their blood circulation and biodistribution. The in vitro phagocytosis results using murine peritoneal macrophages showed 10-fold reduction compared with a reference micelle. The in vivo blood circulation half-life of the polymeric micelles following intravenous administration in New Zealand Rabbits was increased from 0.55 h to 90.5h. More interestingly, tissue distribution results showed that the concentration of the micelles in the kidney is 4-fold higher than that in the liver and other organs 48 h after administration. The results of this work show great promise for designing more effective stealth drug carriers that can minimize reticuloendothelial system clearance and circulate for long time to reach target by using simple cell membrane mimetic random copolymer micelles.
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, PR China; School of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an 710048, Shaanxi, PR China.
authors: Jing
authors: J
authors: Zhao
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, PR China.
authors: Yu-Dong
authors: YD
authors: Chai
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, PR China.
authors: Jing
authors: J
authors: Zhang
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, PR China.
authors: Peng-Fei
authors: PF
authors: Huang
authors: Department of Chemistry, Faculty of Science and Engineering, Saga University, 1 Honjo-machi, Saga 840-8502, Japan. Electronic address: nakashik@cc.saga-u.ac.jp.
authors: Kenichi
authors: K
authors: Nakashima
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, Shaanxi, PR China. Electronic address: gongyk@nwu.edu.cn.
authors: Yong-Kuan
authors: YK
authors: Gong
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003374:Coumarins / Q000494:pharmacology; D005453:Fluorescence; D006207:Half-Life; D057446:Hydrodynamics; D007477:Ions; D008297:Male; D008807:Mice, Inbred BALB C; D008823:Micelles; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D010767:Phosphorylcholine / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D012542:Scattering, Radiation; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D003374:Coumarins; D007477:Ions; D008823:Micelles; D011108:Polymers; D013501:Surface-Active Agents; D010767:Phosphorylcholine; C030123:coumarin
keywords: Biodistribution; Cell membrane mimetic; Long-circulation; Phosphorylcholine; Polymeric micelles