title: Polymorphisms within angiotensin II receptor type 1 gene associated with essential hypertension in Chinese Hani and Yi minorities.
abstract: BACKGROUND
  Angiotensin II receptor type 1 mediates the major cardiovascular effects of angiotensin II to regulate blood pressure. Polymorphisms of angiotensin II receptor type 1 are associated with essential hypertension, but the results are inconsistent and conflicting. The aim of the present study is to assess the association between angiotensin II receptor type 1 polymorphisms and essential hypertension risk in Chinese Hani and Yi minorities.
  
  
  METHODS
  This study recruited 692 unrelated Chinese Hani subjects (case vs. control = 346:346) and 615 unrelated Chinese Yi subjects (case vs. control = 303:312). Twelve selected single nucleotide polymorphisms in the angiotensin II receptor type 1 gene were genotyped using a polymerase chain reaction-restriction fragment length polymorphism method.
  
  
  RESULTS
  Statistical analysis indicated that the GC+CC genotype of rs387967 was significantly associated with the decreased susceptibility to essential hypertension compared with GG in a Yi population (odds ratio = 0.58, 95% confidence intervals 0.41-0.83, P = 0.003). Allele C was a protective allele for essential hypertension (odds ratio = 0.78, 95% confidence intervals 0.61-0.99, P = 0.040). This association was confirmed respectively by comparing systolic blood pressure and diastolic blood pressure between different genotypes and between different alleles, which indicated that the genotype (GC+CC) had a tendency of lower systolic blood pressure and diastolic blood pressure than GG (PSBP = 3.716 × 10(-4), PDBP = 1.187 × 10(-3)); Carriers with C had lower systolic blood pressure and diastolic blood pressure (PSBP = 7.301 × 10(-3), PDBP = 9.142 × 10(-4)). Another single nucleotide polymorphism (rs2638360) was analysed in a Hani minority, then replicated in a Yi minority. The C allele showed a consistent risk trend for essential hypertension in two independent populations (Hani: odds ratio = 1.74, 95% confidence intervals 1.01-2.99, P = 0.046; Yi: odds ratio = 1.27, 95% confidence intervals 0.82-1.96, P = 0.277). Meta-analysis revealed that the C allele could significantly increase the risk of essential hypertension (odds ratio = 1.44, 95% confidence intervals 1.02-2.02, P = 0.037).
  
  
  CONCLUSIONS
  Our findings suggest that rs387967 is associated with the susceptibility to essential hypertension in a Yi population and the tendency was replicated in systolic blood pressure and diastolic blood pressure detection. Meta-analysis revealed that C allele of rs2638360 could significantly increase the risk of essential hypertension. The two single nucleotide polymorphisms maybe play a role in the pathology of essential hypertension.
authors: The First Affiliated Hospital of Kunming Medical University, China.
authors: Hongju
authors: H
authors: Yang
authors: The First Affiliated Hospital of Kunming Medical University, China.
authors: Song
authors: S
authors: Bai
authors: Cell Biology and Genetics Department, Kunming Medical University, China.
authors: Yanrui
authors: Y
authors: Wu
authors: Human Genetics Center of Yunnan University, China.
authors: Qian
authors: Q
authors: Li
authors: Human Genetics Center of Yunnan University, China.
authors: Fangyu
authors: F
authors: Luo
authors: Human Genetics Center of Yunnan University, China.
authors: Bai
authors: B
authors: Li
authors: The First Affiliated Hospital of Kunming Medical University, China.
authors: Yanfen
authors: Y
authors: Jin
authors: Human Genetics Center of Yunnan University, China chunjiexiao123@163.com.
authors: Chunjie
authors: C
authors: Xiao
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics; D000075222:Essential Hypertension; D005260:Female; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008913:Minority Groups; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*
chemical_list: D044140:Receptor, Angiotensin, Type 1
keywords: Single nucleotide polymorphism; angiotensin II receptor type 1; association study; essential hypertension