title: Mitochondria-targeted RNA import.
abstract: The import of a modest number of nucleus-encoded RNAs into mitochondria has been reported in species ranging from yeast to human. With the advent of high-throughput RNA sequencing, additional nucleus-encoded mitochondrial RNAs are being identified. Confirming the mitochondrial localization of candidate RNAs of interest (e.g., small noncoding RNAs, miRNAs, tRNAs, and possibly lncRNAs and viral RNAs) and understanding their function within the mitochondrion is assisted by in vitro and in vivo import assay systems. Here we describe these two systems for studying mitochondrial RNA import, processing, and functions.
authors: School of Life Sciences, Tsinghua University, Beijing, 100084, China.
authors: Geng
authors: G
authors: Wang
authors: Eriko
authors: E
authors: Shimada
authors: Mahta
authors: M
authors: Nili
authors: Carla M
authors: CM
authors: Koehler
authors: Michael A
authors: MA
authors: Teitell
mesh_terms: D002474:Cell-Free System; D066298:In Vitro Techniques; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D034443:RNA Transport; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA
references: Genet Eng (N Y). 2002;24:191-213
references: 12416306
references: Cell. 1989 Jan 13;56(1):131-9
references: 2910496
references: Methods Enzymol. 2013;530:227-51
references: 24034325
references: Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4840-5
references: 22411789
references: Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):998-1007
references: 22023881
references: Cell. 2010 Aug 6;142(3):456-67
references: 20691904