title: Characterization of mitochondrial ATPase 6/8 genes in wild Labeo calbasu (Hamilton, 1822) and mapping of natural genetic diversity.
abstract: We characterized mitochondrial ATP synthase (ATPase) 6 and 8 genes in Labeo calbasu (Hamilton, 1822) and determined genetic variation in wild populations across the natural distribution in Indian rivers. A total of 206 individuals were sampled from 11 riverine sites belonging to distinct geographical locations covering five major river basins. Sequencing of 842 base pairs of ATPase 6/8 revealed 21 haplotypes with haplotype diversity ranging from 0.1250 (River Satluj) to 0.8846 (River Bhagirathi). Analysis of molecular variance (AMOVA) of mitochondrial DNA (mtDNA) data revealed significant genetic differentiation among sites (FST = 0.192, p < 0.0001) which was indicative of moderate level of genetic structuring in the wild L. calbasu populations. The patterns of genetic divergence and haplotype network of mtDNA revealed distinct clades present in Indian rivers. The analysis of data demonstrated the potential of ATPase 6/8 genes in determining the genetic diversity and indicated considerable sub-structuring in wild calbasu populations present in different rivers.
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: Rajeev K
authors: RK
authors: Singh
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: Kuldeep K
authors: KK
authors: Lal
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: Vindhya
authors: V
authors: Mohindra
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: Rama S
authors: RS
authors: Sah
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: Rajesh
authors: R
authors: Kumar
authors: a Fish Conservation Division, NBFGR , Lucknow , UP , India.
authors: J K
authors: JK
authors: Jena
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D054629:Genome, Mitochondrial; D006239:Haplotypes; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D045483:Rivers
chemical_list: C412204:ATP synthase subunit 6; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATPase 6/8; Labeo calbasu; genetic divergence; mtDNA