title: Shifting gear in antimicrobial and anticancer peptides biophysical studies: from vesicles to cells.
abstract: Despite the intensive study on the mechanism of action of membrane-active molecules such as antimicrobial and anticancer peptides, most of the biophysical work has been performed using artificial model systems, mainly lipid vesicles. The use of these systems allows full control of the experimental parameters, and to obtain molecular-level detail on the action of peptides, the correlation with biological action is intangible. Recently, several biophysical methodologies have been translated to studies using bacterial and cancer cells. Here, we review biophysical studies on the mechanism of action of antimicrobial and anticancer peptides performed directly on cells. The data in these studies allow to correlate vesicle-based and cell-based studies and fill the vesicle-cell interdisciplinary gap.
authors: Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028, Lisboa, Portugal.
authors: João M
authors: JM
authors: Freire
authors: Diana
authors: D
authors: Gaspar
authors: Ana Salomé
authors: AS
authors: Veiga
authors: Miguel A R B
authors: MA
authors: Castanho
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002623:Chemistry Techniques, Analytical; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D008051:Lipid Bilayers; D008081:Liposomes
keywords: anticancer; antimicrobial; bacteria; biophysics; cell; lipid; membrane; peptide; spectroscopy; vesicle