title: Tracing the radiation of Maniola (Nymphalidae) butterflies: new insights from phylogeography hint at one single incompletely differentiated species complex.
abstract: The use of DNA sequence data often leads to the recognition of cryptic species within putatively well-known taxa. The opposite case, detecting less diversity than originally described, has, however, far more rarely been documented. Maniola jurtina, the Meadow Brown butterfly, occurs all over Europe, whereas all other six species in the genus Maniola are restricted to the Mediterranean area. Among them, three are island endemics on Sardinia, Cyprus, and Chios, respectively. Maniola species are almost indistinguishable morphologically, and hybridization seems to occur occasionally. To clarify species boundaries and diversification history of the genus, we reconstructed the phylogeography and phylogeny of all seven species within Maniola analyzing 138 individuals from across its range using mitochondrial and nuclear genetic markers. Examination of variation in mitochondrial and nuclear DNA surprisingly revealed a case of taxonomic "oversplitting". The topology of the recovered phylogenetic tree is not consistent with accepted taxonomy, but rather reveals haplotype clades that are incongruent with nominal species boundaries: instead of seven species, we recognized only two major, yet incompletely segregated, lineages. Our results are consistent with the hypothesis that Maniola originated in Africa. We suggest that one lineage dispersed over the Strait of Gibraltar and the Iberian Peninsula to the west of Europe, while the other lineage spreads eastward through Asia Minor and over the Bosporus to Eastern Europe.
authors: Department of Botany and Biodiversity Research, University of Vienna Vienna, Austria.
authors: Angelina J
authors: AJ
authors: Kreuzinger
authors: Department of Botany and Biodiversity Research, University of Vienna Vienna, Austria.
authors: Konrad
authors: K
authors: Fiedler
authors: Department of Botany and Biodiversity Research, University of Vienna Vienna, Austria.
authors: Harald
authors: H
authors: Letsch
authors: Department of Botany and Biodiversity Research, University of Vienna Vienna, Austria.
authors: Andrea
authors: A
authors: Grill
keywords: Biogeography; DNA barcoding; endemism; expansion routes; phylogeny; speciation; species delimitation
references: Nat Commun. 2011;2:324
references: 21610727
references: Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73
references: 291943
references: Mol Biol Evol. 1999 Jan;16(1):37-48
references: 10331250
references: Mol Ecol. 2012 Apr;21(8):1864-77
references: 21883587
references: Mol Phylogenet Evol. 2006 Jul;40(1):29-49
references: 16563805
references: Mol Ecol. 2005 Jul;14(8):2433-43
references: 15969725
references: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):968-71
references: 16418261
references: Mol Phylogenet Evol. 2013 Jan;66(1):316-26
references: 23085677
references: Mol Biol Evol. 1995 Jul;12(4):650-6
references: 7659020
references: Heredity (Edinb). 1997 Mar;78 ( Pt 3):311-27
references: 9119706
references: J Evol Biol. 2007 Jul;20(4):1255-70
references: 17584221
references: Mol Phylogenet Evol. 2009 Nov;53(2):463-78
references: 19580878
references: J Evol Biol. 2007 Nov;20(6):2181-91
references: 17887973
references: Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14812-7
references: 15465915
references: Insect Mol Biol. 1998 Feb;7(1):73-82
references: 9459431
references: PLoS One. 2013 Jul 08;8(7):e66213
references: 23861743
references: PLoS One. 2011 Feb 14;6(2):e17134
references: 21423340
references: Mol Ecol. 2012 Sep;21(18):4422-36
references: 22891635
references: Bioinformatics. 2009 Jun 1;25(11):1451-2
references: 19346325
references: Nucleic Acids Res. 2011 Jul;39(Web Server issue):W470-4
references: 21646336
references: Cladistics. 2013 Apr;29(2):166-192
references: 34818826
references: Bioinformatics. 2001 Aug;17(8):754-5
references: 11524383
references: Mol Phylogenet Evol. 2001 Feb;18(2):264-81
references: 11161761
references: Mol Biol Evol. 2011 Oct;28(10):2731-9
references: 21546353