title: Pi64, Encoding a Novel CC-NBS-LRR Protein, Confers Resistance to Leaf and Neck Blast in Rice.
abstract: Rice blast caused by Magnaporthe oryzae poses a major threat to rice production worldwide. The utilization of host resistance (R) genes is considered to be the most effective and economic means to control rice blast. Here, we show that the japonica landrace Yangmaogu (YMG) displays a broader spectrum of resistance to blast isolates than other previously reported broad-spectrum resistant (BSR) cultivars. Genetic analysis suggested that YMG contains at least three major R genes. One gene, Pi64, which exhibits resistance to indica-sourced isolate CH43 and several other isolates, was mapped to a 43-kb interval on chromosome 1 of YMG. Two open reading frames (NBS-1 and NBS-2) encoding nucleotide-binding site and leucine-rich repeat proteins were short-listed as candidate genes for Pi64. Constructs containing each candidate gene were transformed into three susceptible japonica cultivars. Only transformants with NBS-2 conferred resistance to leaf and neck blast, validating the idea that NBS-2 represents the functional Pi64 gene. Pi64 is constitutively expressed at all development stages and in all tissues examined. Pi64 protein is localized in both the cytoplasm and nucleus. Furthermore, introgression of Pi64 into susceptible cultivars via gene transformation and marker-assisted selection conferred high-level and broad-spectrum leaf and neck blast resistance to indica-sourced isolates, demonstrating its potential utility in breeding BSR rice cultivars.
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Jian
authors: J
authors: Ma
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Cailin
authors: C
authors: Lei
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Xingtao
authors: X
authors: Xu
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Kun
authors: K
authors: Hao
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Jiulin
authors: J
authors: Wang
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Zhijun
authors: Z
authors: Cheng
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Xiaoding
authors: X
authors: Ma
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Jin
authors: J
authors: Ma
authors: 2National Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
authors: Kunneng
authors: K
authors: Zhou
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Xin
authors: X
authors: Zhang
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Xiuping
authors: X
authors: Guo
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Fuqing
authors: F
authors: Wu
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Qibing
authors: Q
authors: Lin
authors: 2National Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
authors: Chunming
authors: C
authors: Wang
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Huqu
authors: H
authors: Zhai
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
authors: Haiyang
authors: H
authors: Wang
authors: 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;|2National Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jianmin
authors: J
authors: Wan
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001947:Breeding; D002874:Chromosome Mapping; D060467:Disease Resistance; D017930:Genes, Reporter; D000090263:Leucine-Rich Repeat Proteins; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011523:Protoplasts; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins