title: Laboratory recommendations for scoring deep molecular responses following treatment for chronic myeloid leukemia.
abstract: Treatment of chronic myeloid leukemia (CML) with tyrosine kinase inhibitors has advanced to a stage where many patients achieve very low or undetectable levels of disease. Remarkably, some of these patients remain in sustained remission when treatment is withdrawn, suggesting that they may be at least operationally cured of their disease. Accurate definition of deep molecular responses (MRs) is therefore increasingly important for optimal patient management and comparison of independent data sets. We previously published proposals for broad standardized definitions of MR at different levels of sensitivity. Here we present detailed laboratory recommendations, developed as part of the European Treatment and Outcome Study for CML (EUTOS), to enable testing laboratories to score MR in a reproducible manner for CML patients expressing the most common BCR-ABL1 variants.
authors: 1] Faculty of Medicine, University of Southampton, Southampton, UK [2] National Genetics Reference Laboratory (Wessex), Salisbury District Hospital, Salisbury, UK.
authors: N C P
authors: NC
authors: Cross
authors: 1] Faculty of Medicine, University of Southampton, Southampton, UK [2] National Genetics Reference Laboratory (Wessex), Salisbury District Hospital, Salisbury, UK.
authors: H E
authors: HE
authors: White
authors: Hematopathology Unit, Hospital Clinic, IDIBAPS, Barcelona, Spain.
authors: D
authors: D
authors: Colomer
authors: Department of Clinical Genetics, Laboratory Medicine, Lund University, Lund, Sweden.
authors: H
authors: H
authors: Ehrencrona
authors: Imperial Molecular Pathology Laboratory, Centre for Haematology, Imperial College London, London, UK.
authors: L
authors: L
authors: Foroni
authors: Department of Clinical and Biological Science, University of Turin, Turin, Italy.
authors: E
authors: E
authors: Gottardi
authors: Abteilung für Hämatologie und internistische Onkologie, Universität Leipzig, Leipzig, Germany.
authors: T
authors: T
authors: Lange
authors: Children's Cancer Research Institute/LabDia Labordiagnostik and Medical University, Vienna, Austria.
authors: T
authors: T
authors: Lion
authors: Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
authors: K
authors: K
authors: Machova Polakova
authors: Laboratoire Hematologie, CHU Bordeaux, Hematopoiese Leucemique et Cibles Therapeutiques, INSERM U1035, Université Bordeaux, Bordeaux, France.
authors: S
authors: S
authors: Dulucq
authors: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
authors: G
authors: G
authors: Martinelli
authors: Molecular Diagnostics Laboratory, Department of Hematology, University Hospital Bern, Bern, Switzerland.
authors: E
authors: E
authors: Oppliger Leibundgut
authors: Klinisk Biokemi, Vejle Sygehus, Vejle, Denmark.
authors: N
authors: N
authors: Pallisgaard
authors: Department of Molecular Medicine and Surgery, Clinical Genetics, Karolinska Institutet, Stockholm, Sweden.
authors: G
authors: G
authors: Barbany
authors: Hematology Department, Jagiellonian University, Krakow, Poland.
authors: T
authors: T
authors: Sacha
authors: Hematology Department - Fundeni Clinical Institute, University of Medicine and Pharmacy 'Carol Davila', Bucharest, Romania.
authors: R
authors: R
authors: Talmaci
authors: Department of Clinical Medicine and Surgery, University 'Federico II' of Naples, Naples, Italy.
authors: B
authors: B
authors: Izzo
authors: Department of Clinical and Biological Science, University of Turin, Turin, Italy.
authors: G
authors: G
authors: Saglio
authors: 1] Department of Clinical Medicine and Surgery, University 'Federico II' of Naples, Naples, Italy [2] CEINGE - Biotecnologie Avanzate, Naples, Italy.
authors: F
authors: F
authors: Pane
authors: III. Medizinische Klinik, Universitätsmedizin Mannheim, Mannheim, Germany.
authors: M C
authors: MC
authors: Müller
authors: Abteilung Hämatologie/Onkologie, Klinik für Innere Medizin II, Universitätsklinikum Jena, Jena, Germany.
authors: A
authors: A
authors: Hochhaus
mesh_terms: D002138:Calibration; D005060:Europe; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D014644:Genetic Variation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000378:metabolism*; D057230:Limit of Detection; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D016896:Treatment Outcome
chemical_list: C581634:BCR-ABL1 fusion protein, human; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl
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