title: Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation.
abstract: A hydroponics experiment was aimed at identifying the lead (Pb) tolerance and phytoremediation potential of Moso bamboo (Phyllostachys pubescens) seedlings grown under different Pb treatments. Experimental results indicated that at the highest Pb concentration (400 μmol/L), the growth of bamboo seedlings was inhibited and Pb concentrations in leaves, stems, and roots reached the maximum of 148.8, 482.2, and 4282.8 mg/kg, respectively. Scanning electron microscopy revealed that the excessive Pb caused decreased stomatal opening, formation of abundant inclusions in roots, and just a few inclusions in stems. The ultrastructural analysis using transmission electron microscopy revealed that the addition of excessive Pb caused abnormally shaped chloroplasts, disappearance of endoplasmic reticulum, shrinkage of nucleus and nucleolus, and loss of thylakoid membranes. Although ultrastructural analysis revealed some internal damage, even the plants exposed to 400 µmol/L Pb survived and no visual Pb toxicity symptoms such as necrosis and chlorosis were observed in these plants. Even at the highest Pb treatment, no significant difference was observed for the dry weight of stem compared with controls. It is suggested that use of Moso bamboo as an experimental material provides a new perspective for remediation of heavy metal contaminated soil owing to its high metal tolerance and greater biomass.
authors: Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, School of Environmental and Resource Sciences, Zhejiang A & F University, Lin'an 311300, China; National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000, Pakistan.
authors: Dan
authors: D
authors: Liu
authors: Song
authors: S
authors: Li
authors: Ejazul
authors: E
authors: Islam
authors: Jun-ren
authors: JR
authors: Chen
authors: Jia-sen
authors: JS
authors: Wu
authors: Zheng-qian
authors: ZQ
authors: Ye
authors: Dan-li
authors: DL
authors: Peng
authors: Wen-bo
authors: WB
authors: Yan
authors: Kou-ping
authors: KP
authors: Lu
mesh_terms: D001673:Biodegradation, Environmental; D004361:Drug Tolerance / Q000502:physiology; D007854:Lead / Q000493:pharmacokinetics* / Q000494:pharmacology*; D031787:Sasa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D036226:Seedlings / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012989:Soil Pollutants / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D012989:Soil Pollutants; D007854:Lead
keywords: Moso bamboo; Pb; Phytoremediation; Scanning electron microscopy; Transmission electron microscopy
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