title: The neostriatum: two entities, one structure?
abstract: The striosome (or patch) was first identified with anatomical techniques as neurons organized in a three-dimensional labyrinth inserted in and interdigitating the rest of neostriatum: the matrix. Striosome and matrix rapidly became known as two neuronal compartments expressing different biochemical markers, embryonic development and afferent and efferent connectivity. In spite of extensive intrinsic neuronal axonal and dendritic extensions supposed to exchange information between matrix and striosomes, evidence suggested the presence of independent areas. Here, we report that indeed these two areas do not exchange synaptic information. We used genetic expression of channel rhodopsin 2 carried by adeno-associated virus serotype 10 (AAVrh10) that only expresses in neurons of the matrix compartment. Whole-cell patch-clamp recordings of matrix neurons activated by light pulses consistently produced inhibitory postsynaptic currents (IPSCs), but the same manipulation did not evoke IPSCs in striosome neurons. The matrix contains both direct and indirect striatal output pathways. By targeting striatal matrix expression of designer receptors exclusively activated by a designer drug (DREADD) hM3di carried by AAVrh10, we were able to inhibit the matrix neuronal compartment of the dorsolateral striatum during performance of a learned single-pellet reach-to-grasp task. As expected, inhibition of matrix neurons by systemic administration of DREADD agonist clozapine-n-oxide interfered with performance of the learned task.
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan.
authors: Violeta G
authors: VG
authors: Lopez-Huerta
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan.
authors: Yoko
authors: Y
authors: Nakano
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan.
authors: Johannes
authors: J
authors: Bausenwein
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan.
authors: Omar
authors: O
authors: Jaidar
authors: University of Tsukuba, Ibaraki, 305-0006, Japan.
authors: Michael
authors: M
authors: Lazarus
authors: University of Tsukuba, Ibaraki, 305-0006, Japan.
authors: Yoan
authors: Y
authors: Cherassse
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan.
authors: Marianela
authors: M
authors: Garcia-Munoz
authors: Brain Mechanisms for Behavior Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son, Kunigami Gun, Okinawa, 904-0412, Japan. gordon@oist.jp.
authors: Gordon
authors: G
authors: Arbuthnott
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D003024:Clozapine / Q000008:administration & dosage / Q000031:analogs & derivatives; D015198:Designer Drugs / Q000008:administration & dosage; D005260:Female; D053444:Inhibitory Postsynaptic Potentials; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009043:Motor Activity / Q000187:drug effects; D017072:Neostriatum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D015198:Designer Drugs; D003024:Clozapine; C079149:clozapine N-oxide
keywords: Adeno-associated virus; Dopamine; Limbic system; Neuromodulation; Reach-to-grasp; Volume transmission
references: Brain. 1986 Oct;109 ( Pt 5):805-43
references: 3779371
references: Neuroscience. 1999;89(4):1009-23
references: 10362291
references: Mol Ther. 2006 Mar;13(3):528-37
references: 16413228
references: Nature. 1981 Jun 4;291(5814):415-8
references: 6165892
references: Science. 1989 Oct 20;246(4928):385-8
references: 2799392
references: J Neurosci. 1988 Mar;8(3):892-900
references: 3346727
references: Proc Natl Acad Sci U S A. 1978 Nov;75(11):5723-6
references: 103101
references: Trends Neurosci. 2007 May;30(5):203-10
references: 17400301
references: Neuroscience. 1993 Dec;57(3):661-71
references: 8309529
references: Z Anat Entwicklungsgesch. 1973 Aug 30;140(3):281-318
references: 4749135
references: Behav Brain Res. 2006 Nov 25;175(1):82-9
references: 16956678
references: Brain Res. 1985 Dec 2;348(2):220-8
references: 3907747
references: Int Rev Immunol. 2014 Jan;33(1):45-53
references: 24127823
references: J Comp Neurol. 1988 Mar 8;269(2):275-89
references: 2833538
references: Can J Physiol Pharmacol. 2004 Aug-Sep;82(8-9):732-9
references: 15523530
references: Annu Rev Neurosci. 2007;30:259-88
references: 17600522
references: Neuroscience. 1988 Mar;24(3):853-75
references: 2454418
references: Exp Brain Res. 1979 Jan 2;34(1):189-95
references: 759226
references: Neuroscience. 2009 Sep 29;163(1):109-19
references: 19463902
references: Brain Res Dev Brain Res. 1990 Dec 1;57(1):93-102
references: 1965303
references: Behav Brain Res. 2006 Jan 30;166(2):189-96
references: 16153716
references: J Neurosci Methods. 1994 Jun;52(2):169-74
references: 7967719
references: Neuroscience. 1989;32(2):297-321
references: 2479881
references: Neuroscience. 1991;40(3):725-33
references: 1676494
references: Proc Natl Acad Sci U S A. 1989 Nov;86(22):9020-4
references: 2813436
references: Exp Eye Res. 2010 Nov;91(5):652-9
references: 20723541
references: J Neurosci. 2003 Jan 15;23(2):579-86
references: 12533618
references: J Neurosci. 2002 Jan 1;22(1):305-14
references: 11756514
references: Trends Pharmacol Sci. 2012 Aug;33(8):442-8
references: 22621975
references: Neuroreport. 1996 Sep 2;7(13):2119-24
references: 8930971
references: Nat Neurosci. 2005 Sep;8(9):1263-8
references: 16116447
references: J Neurophysiol. 2002 Sep;88(3):1263-9
references: 12205147
references: J Neurosci. 1994 Nov;14(11 Pt 2):7148-58
references: 7965105
references: J Neurosci Methods. 2010 Oct 30;193(1):82-5
references: 20800620
references: Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8
references: 17360345
references: Curr Opin Neurobiol. 2014 Apr;25:194-200
references: 24566242
references: Nature. 1986 Oct 16-22;323(6089):625-7
references: 3773990
references: Nat Neurosci. 2000 Apr;3(4):377-83
references: 10725928
references: J Neurosci. 1987 Dec;7(12):3915-34
references: 2891799
references: Nature. 1984 Oct 4-10;311(5985):461-4
references: 6207434
references: Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):774-9
references: 16407107
references: J Neurosci. 2011 Jan 26;31(4):1183-92
references: 21273403
references: Brain Res. 1972 Sep 15;44(1):283-8
references: 4403485
references: Neuropsychologia. 1981;19(3):463-7
references: 7266840
references: Mol Ther. 2008 Oct;16(10):1710-8
references: 18714307
references: Neurosci Lett. 2005 Sep 16;385(3):198-203
references: 15970379
references: Brain Res. 1996 Jan 29;707(2):221-7
references: 8919299
references: Gene Ther. 2014 Jan;21(1):28-36
references: 24131981
references: J Comp Neurol. 1992 Jun 15;320(3):304-22
references: 1351896
references: Neuroscience. 1986 Mar;17(3):547-71
references: 2422590
references: J Comp Neurol. 1991 Sep 1;311(1):134-67
references: 1719043
references: Neuroscience. 1981;6(3):377-97
references: 6164013
references: Gene Ther. 2005 Jun;12(11):873-80
references: 15829993
references: Vision Res. 2008 Feb;48(3):353-9
references: 17923143
references: Front Neural Circuits. 2012 Jun 27;6:38
references: 22754501
references: Proc Natl Acad Sci U S A. 1985 Dec;82(24):8780-4
references: 3909155
references: J Neurosci. 1995 Sep;15(9):5999-6013
references: 7666184
references: J Neurophysiol. 1991 Oct;66(4):1249-63
references: 1722244
references: J Neurosci. 1987 Dec;7(12):3935-44
references: 2891800
references: Behav Neurosci. 1988 Jun;102(3):429-40
references: 3395453
references: Nat Methods. 2006 Oct;3(10):785-92
references: 16990810
references: J Chem Neuroanat. 1994 Aug;7(3):191-201
references: 7531455
references: Neuropharmacology. 2014 Oct;85:451-60
references: 24951857
references: J Neurophysiol. 1989 Nov;62(5):1052-68
references: 2585039
references: J Comp Neurol. 1993 Nov 22;337(4):614-28
references: 8288774
references: Neuropsychopharmacology. 2010 Jan;35(1):48-69
references: 19776734
references: Neuroscience. 1995 Nov;69(1):71-81
references: 8637634
references: Anat Rec A Discov Mol Cell Evol Biol. 2004 Jul;279(1):583-91
references: 15224400
references: Brain Res. 1987 Jan 13;401(1):155-61
references: 3028569
references: J Neurosci. 2001 Sep 15;21(18):7247-60
references: 11549735
references: J Neurosci. 1993 Mar;13(3):1120-37
references: 7680067
references: Annu Rev Neurosci. 2008;31:359-87
references: 18558860
references: Prog Neurobiol. 1996 Nov;50(4):381-425
references: 9004351
references: Virol J. 2013;10:74
references: 23497173
references: J Virol. 2004 May;78(9):4421-32
references: 15078923
references: Neuroscience. 1995 Apr;65(3):709-30
references: 7609871
references: Eur J Neurosci. 2008 Apr;27(7):1615-25
references: 18380664
references: Eur J Neurosci. 2011 Feb;33(4):668-77
references: 21314848
references: J Physiol. 1991 Apr;435:275-93
references: 1837562
references: Brain Res. 1987 Oct 13;423(1-2):329-32
references: 3499959
references: J Neurosci. 2011 Jul 6;31(27):10067-75
references: 21734299
references: Curr Biol. 2012 Oct 23;22(20):1918-24
references: 23000149
references: Curr Gene Ther. 2005 Jun;5(3):299-310
references: 15975007
references: J Comp Neurol. 1990 Dec 8;302(2):197-205
references: 2289971
references: Trends Neurosci. 2000 Oct;23(10 Suppl):S71-7
references: 11052223
references: Eur J Neurosci. 1999 Feb;11(2):481-90
references: 10051749
references: Front Neuroanat. 2011 Sep 07;5:59
references: 21941467
references: Brain Res Rev. 2008 Aug;58(2):272-81
references: 18054796
references: Proc Natl Acad Sci U S A. 1976 Oct;73(10):3729-33
references: 185626
references: Brain Res. 1986 Feb 19;365(2):397-403
references: 3004664
references: J Comp Neurol. 1996 Oct 28;374(4):578-92
references: 8910736
references: Neurosci Lett. 2002 Oct 25;332(1):5-8
references: 12377371
references: Proc Natl Acad Sci U S A. 1998 May 26;95(11):6486-91
references: 9600993
references: Drug Discov Today. 2014 Apr;19(4):469-73
references: 24184433
references: J Neurosci. 1990 Oct;10(10):3421-38
references: 1698947
references: Genomics. 2004 May;83(5):790-801
references: 15081109
references: Neuron. 2012 Jun 7;74(5):858-73
references: 22681690
references: Trends Neurosci. 2004 May;27(5):270-7
references: 15111009
references: Science. 2007 Sep 7;317(5843):1355
references: 17690262
references: J Neurophysiol. 1992 Jun;67(6):1669-82
references: 1352806
references: J Neurosci. 1991 Mar;11(3):779-91
references: 1705968
references: J Comp Neurol. 2009 Jun 10;514(5):507-17
references: 19350670
references: J Virol. 1999 May;73(5):3994-4003
references: 10196295
references: J Comp Neurol. 1993 Nov 22;337(4):629-39
references: 8288775
references: Prog Brain Res. 2000;125:303-8
references: 11098666