title: Impact of dietary fatty acids on muscle composition, liver lipids, milt composition and sperm performance in European eel.
abstract: In order for European eel aquaculture to be sustainable, the life cycle should be completed in captivity. Development of broodstock diets may improve the species' reproductive success in captivity, through the production of high-quality gametes. Here, our aim was to evaluate the influence of dietary regime on muscle composition, and liver lipids prior to induced maturation, and the resulting sperm composition and performance. To accomplish this fish were reared on three "enhanced" diets and one commercial diet, each with different levels of fatty acids, arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Neutral lipids from the muscle and liver incorporated the majority of the fatty acid profile, while phospholipids incorporated only certain fatty acids. Diet had an effect on the majority of sperm fatty acids, on the total volume of extractable milt, and on the percentage of motile sperm. Here, our results suggest that the total volume of extractable milt is a DHA-dependent process, as we found the diets with the highest DHA levels induced the most milt while the diet with the lowest DHA level induced the least amount of milt. The diet with the highest level of ARA induced medium milt volumes but had the highest sperm motility. EPA also seems important for sperm quality parameters since diets with higher EPA percentages had a higher volume of milt and higher sperm motility. In conclusion, dietary fatty acids had an influence on fatty acids in the tissues of male eel and this impacted sperm performance.
authors: Technical University of Denmark, National Institute of Aquatic Resources, Jægersborg Allé 1, 2920 Charlottenlund, Denmark. Electronic address: ianb@aqua.dtu.dk.
authors: Ian Anthony Ernest
authors: IA
authors: Butts
authors: Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.
authors: Rosa
authors: R
authors: Baeza
authors: Technical University of Denmark, National Institute of Aquatic Resources, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.
authors: Josianne Gatt
authors: JG
authors: Støttrup
authors: Technical University of Denmark, National Institute of Aquatic Resources, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.
authors: Maria
authors: M
authors: Krüger-Johnsen
authors: National Food Institute, Technical University of Denmark, Lyngby, Denmark.
authors: Charlotte
authors: C
authors: Jacobsen
authors: Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.
authors: Luz
authors: L
authors: Pérez
authors: Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.
authors: Juan F
authors: JF
authors: Asturiano
authors: Technical University of Denmark, National Institute of Aquatic Resources, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.
authors: Jonna
authors: J
authors: Tomkiewicz
mesh_terms: D000811:Anguilla / Q000502:physiology*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D009132:Muscles / Q000737:chemistry* / Q000187:drug effects; D012661:Semen / Q000737:chemistry* / Q000187:drug effects; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid
keywords: Arachidonic acid; Broodstock diet; Docosahexaenoic acid; Eicosapentaenoic acid; Fatty acid composition; Sperm