title: Daily variations in the expression of miR-16 and miR-181a in human leukocytes.
abstract: Circadian rhythms are controlled by a molecular mechanism that is organized in transcriptional and translational feedback loops of gene expression. Recent studies have been demonstrating the involvement of microRNAs (miRs) in post-transcriptional/translational control of circadian rhythms. In the present study we aimed to analyze the daily variations of miR-16 and miR-181a expression in human leukocytes. These miRs were independently associated with hematopoiesis and circadian rhythms in previous studies using experimental models. Peripheral blood from 6 subjects was sampled in a 24 hour period for expression analysis using quantitative real-time PCR (RT-qPCR). Initially, we evaluated the expression stability of RNU6-2, RNU1A-1, RNU5A-1, SNORD-25, SCARNA-17 and SNORA-73A as candidate genes for normalization of RT-qPCR data. The combination of the four most stable genes (SNORA-73A/SCARNA-17/SNORD-25/RNU6-2) was indicated to provide a better normalization of miRs expressions. The results show a daily variation of miR-181a and miR-16 expression in human leukocytes, suggesting a potential participation of these genes in the modulation of the circadian rhythms present in blood cells.
authors: Laboratory of Molecular Chronobiology, Universidade Federal de Alagoas, Campus Arapiraca, Alagoas, Brazil.
authors: Diego de Siqueira
authors: Dde S
authors: Figueredo
authors: Laboratory of Cellular and Molecular Biology, Universidade Federal de Alagoas, Maceió, Alagoas, Brazil.
authors: Daniel Leite Góes
authors: DL
authors: Gitaí
authors: Laboratory of Molecular Chronobiology, Universidade Federal de Alagoas, Campus Arapiraca, Alagoas, Brazil. Electronic address: deandrade.tiago@pq.cnpq.br.
authors: Tiago Gomes de
authors: TG
authors: Andrade
mesh_terms: D002940:Circadian Rhythm / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020043:Genes, Essential; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D012015:Reference Standards; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C505362:MIRN16 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D020537:RNA, Small Nucleolar; D012261:Ribonucleoproteins
keywords: Circadian; Hematopoiesis; Housekeeping genes; Human leukocytes; miR