title: Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway.
abstract: The fully grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. Here we show that during the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that was previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR-eIF4F pathway. Here we reveal a mechanism that-following the resumption of meiosis-controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation.
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Andrej
authors: A
authors: Susor
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Denisa
authors: D
authors: Jansova
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Renata
authors: R
authors: Cerna
authors: CEITEC-Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic.
authors: Anna
authors: A
authors: Danylevska
authors: 1] Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic [2] CEITEC-Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic.
authors: Martin
authors: M
authors: Anger
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Tereza
authors: T
authors: Toralova
authors: Institute of Molecular Genetics, ASCR, Videnska 1083, 142 20 Prague, Czech Republic.
authors: Radek
authors: R
authors: Malik
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Jaroslava
authors: J
authors: Supolikova
authors: The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, California 94143, USA.
authors: Matthew S
authors: MS
authors: Cook
authors: Department of Genetic Engineering, Sungkyunkwan University, Gyeonggi-do, Suwon 440-746, South Korea.
authors: Jeong Su
authors: JS
authors: Oh
authors: Institute of Animal Physiology and Genetics, ASCR, Rumburska 89, 277 21 Libechov, Czech Republic.
authors: Michal
authors: M
authors: Kubelka
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000378:metabolism; D015536:Down-Regulation; D039562:Eukaryotic Initiation Factor-4F / Q000378:metabolism*; D005306:Fertilization; D042822:Genomic Instability; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D008540:Meiosis; D051379:Mice; D009685:Nuclear Envelope / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D013997:Time Factors
chemical_list: D039562:Eukaryotic Initiation Factor-4F; D012315:RNA Caps; D012333:RNA, Messenger; D058570:TOR Serine-Threonine Kinases
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