title: Early infectious acute respiratory distress syndrome is characterized by activation and proliferation of alveolar T-cells.
abstract: Acute respiratory distress syndrome (ARDS) in humans is characterized by the infiltration of polymorphonuclears in the alveolar spaces. However, the role of T-cells in ARDS is unknown. Our aim was to characterize the T-cell phenotype in bronchoalveolar lavage (BAL) during the early phase of acute lung infection(ALI)/ARDS-infected patients in comparison to a control group (CG). BAL lymphocyte phenotypes of two ALI, 16 ARDS, and eight CG were examined by flow cytometry. ALI/ARDS showed a significant increase in CD4 and CD8 T-cell activation as compared to CG. Moreover, a significant level of proliferation was observed using the Ki67 marker in ARDS patients as compared to controls (median): 37 versus 6 % for CD4 T-cells (p = 0.022) and 34 versus 2 % for CD8 T-cells (p = 0.009). In contrast, the percentage of T-regulatory cells and apoptotic T-cells were similar in both groups. Among costimulatory molecules, we observed an overexpression of CTLA-4/CD152 on CD4 T-cells in ALI/ARDS as compared to CG: 30 versus 7 %, respectively (p = 0.063). In further characterizing T-cell subsets expressing high levels of CD152, we found the presence of IL-17 secreting CD4 T-cells in ALI/ARDS. In humans, ALI/ARDS due to infection is associated with a high level of T-cell activation and proliferation, along with the presence of Th17 cells, which are known to attract polymorphonuclears.
authors: Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France.
authors: K
authors: K
authors: Risso
authors: G
authors: G
authors: Kumar
authors: M
authors: M
authors: Ticchioni
authors: C
authors: C
authors: Sanfiorenzo
authors: J
authors: J
authors: Dellamonica
authors: F
authors: F
authors: Guillouet-de Salvador
authors: G
authors: G
authors: Bernardin
authors: C-H
authors: CH
authors: Marquette
authors: P-M
authors: PM
authors: Roger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000473:pathology*; D011446:Prospective Studies; D011650:Pulmonary Alveoli / Q000473:pathology*; D012128:Respiratory Distress Syndrome / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000473:pathology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD
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