title: Detection of disseminated tumor cells in aspirative drains after neck dissection.
abstract: The dissemination of individual tumor cells is a common phenomenon in solid cancers. Detection of tumor cells in bone marrow disseminated tumor cells (DTC) and in peripheral blood circulating tumor cells (CTC) in nonmetastatic situation is of high prognostic significance. Compared to breast, colon and prostate cancers, the studies on CTC and DTC in head and neck cancers are sparse. The objective of our study was to detect DTC in drains after neck dissection. Fourteen patients undergoing surgery for stages III and IV head and neck cancers were enrolled in this study--twelve presenting with squamous cell carcinoma and two with adenocarcinoma. Redon drain analysis was performed by the Cellsearch method using immunomagnetic and fluorescence approaches. A positivity threshold value was set at 2DTC/7.5 ml of the sample. Tumor cells were detected in drains of 69 % of patients a few days after surgery. The range of quantification was 3-2,094 DTC/5 ml and we showed morphological differences between the two types of carcinoma cells. DTC were detected after neck dissection both in squamous cell carcinoma and in adenocarcinoma. Potential clinical significance of tumor cells needs to be further investigated as their presence could affect pre-surgical and post-operative treatments.
authors: Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France. r.mastronicola@nancy.unicancer.fr.|CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France. r.mastronicola@nancy.unicancer.fr.|Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France. r.mastronicola@nancy.unicancer.fr.
authors: R
authors: R
authors: Mastronicola
authors: Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: C
authors: C
authors: Berteau
authors: Immunology Laboratory, Nancytomique, CHU de Nancy, Rue du Morvan, Vandœuvre-Lès-Nancy, France.|Faculty of Medicine, EA4369 RHEM Nancy University, 9 Avenue de la Forêt de Haye, Vandœuvre-Lès-Nancy, France.
authors: Q
authors: Q
authors: Tu
authors: Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: S
authors: S
authors: Cortese
authors: Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: J
authors: J
authors: Guillet
authors: Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: B
authors: B
authors: Phulpin
authors: Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: P
authors: P
authors: Gangloff
authors: Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: L
authors: L
authors: Bezdetnaya
authors: Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: J-L
authors: JL
authors: Merlin
authors: Immunology Laboratory, Nancytomique, CHU de Nancy, Rue du Morvan, Vandœuvre-Lès-Nancy, France.|Faculty of Medicine, EA4369 RHEM Nancy University, 9 Avenue de la Forêt de Haye, Vandœuvre-Lès-Nancy, France.
authors: G
authors: G
authors: Faure
authors: Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.|Institut de Cancérologie de Lorraine, 54519, Vandœuvre-Lès-Nancy, France.
authors: G
authors: G
authors: Dolivet
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000556:secondary / Q000601:surgery; D004322:Drainage; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000556:secondary / Q000601:surgery; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D037981:Neck Dissection / Q000379:methods*; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011183:Postoperative Complications; D011379:Prognosis; D000077195:Squamous Cell Carcinoma of Head and Neck
keywords: Cellsearch method; Disseminated tumor cells; Head and neck cancer; Redon drains
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