title: Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates.
abstract: In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates. Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4-producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 β-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicates that blaCMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with blaDHA-1. All isolates carrying blaCMY-4 displayed the transposon-like structures ISEcp1/ΔISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates in Algeria.
authors: Laboratoire de Microbiologie Appliquée, Département de Microbiologie, FSNV, Université de Bejaia, 06000, Algeria.
authors: Alima
authors: A
authors: Gharout-Sait
authors: Laboratoire d'Ecologie Microbienne, Département de Microbiologie, FSNV, Université de Bejaia, 06000, Algeria. Electronic address: ziz1999@yahoo.fr.
authors: Abdelaziz
authors: A
authors: Touati
authors: Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France.
authors: Thomas
authors: T
authors: Guillard
authors: Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France.
authors: Lucien
authors: L
authors: Brasme
authors: Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France; Université de Reims-Champagne-Ardenne, Reims, France.
authors: Christophe
authors: C
authors: de Champs
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002440:Cefoxitin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002440:Cefoxitin; C048795:AmpC beta-lactamases; D001618:beta-Lactamases
keywords: Algeria; AmpC β-lactamases; Enterobacteriaceae; Genotyping
references: Microb Drug Resist. 2006 Spring;12(1):44-9
references: 16584308
references: J Food Prot. 2009 Mar;72(3):624-30
references: 19343954
references: Int J Antimicrob Agents. 2006 May;27(5):397-402
references: 16621456
references: J Clin Microbiol. 2005 Aug;43(8):4178-82
references: 16081970
references: Diagn Microbiol Infect Dis. 2011 Jun;70(2):253-9
references: 21596225
references: Antimicrob Agents Chemother. 2013 Jan;57(1):269-76
references: 23114770
references: Antimicrob Agents Chemother. 2009 Jul;53(7):3126-30
references: 19398647
references: Foodborne Pathog Dis. 2012 Sep;9(9):803-8
references: 22871227
references: Antimicrob Agents Chemother. 2004 Aug;48(8):2845-52
references: 15273090
references: J Microbiol Methods. 2005 Dec;63(3):219-28
references: 15935499
references: J Food Sci. 2009 Sep;74(7):M405-10
references: 19895488
references: Emerg Infect Dis. 2007 Mar;13(3):443-8
references: 17552098
references: Indian J Med Res. 2012;135:114-9
references: 22382192
references: Antimicrob Agents Chemother. 2006 Sep;50(9):3203-6
references: 16940132
references: J Clin Microbiol. 2002 Sep;40(9):3121-6
references: 12202541
references: Environ Microbiol Rep. 2013 Feb;5(1):58-65
references: 23757131
references: Med Mal Infect. 2012 Jan;42(1):20-9
references: 22056377
references: Int J Antimicrob Agents. 2009 Oct;34(4):340-2
references: 19570655
references: BMC Microbiol. 2013 Jun 25;13:147
references: 23800277
references: J Antimicrob Chemother. 2009 Sep;64(3):524-8
references: 19525516
references: J Microbiol Methods. 2014 Jun;101:24-7
references: 24685601
references: Clin Microbiol Infect. 2011 Jul;17(7):1032-6
references: 20673270
references: Clin Microbiol Infect. 2008 Jan;14 Suppl 1:90-103
references: 18154532
references: Antimicrob Agents Chemother. 2006 Feb;50(2):607-17
references: 16436717
references: Rev Esp Quimioter. 2006 Jun;19(2):144-51
references: 16964332
references: Chemotherapy. 2010;56(1):46-53
references: 20203489
references: Antimicrob Agents Chemother. 2002 Jan;46(1):1-11
references: 11751104
references: Int J Antimicrob Agents. 2013 May;41(5):414-20
references: 23434250
references: Antimicrob Agents Chemother. 2012 May;56(5):2705-12
references: 22354297
references: J Med Microbiol. 2014 Oct;63(Pt 10):1316-1323
references: 25009193
references: Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents
references: 19136439
references: Int J Med Microbiol. 2013 Dec;303(8):553-7
references: 23999104
references: J Antimicrob Chemother. 2009 Feb;63(2):274-81
references: 19001452
references: J Clin Microbiol. 2010 Jan;48(1):286-9
references: 19923484
references: Antimicrob Agents Chemother. 2009 Sep;53(9):4002-6
references: 19596889
references: Nat Rev Microbiol. 2010 Mar;8(3):207-17
references: 20157339
references: J Antimicrob Chemother. 2007 Aug;60(2):398-401
references: 17526503
references: J Infect. 2012 Feb;64(2):176-83
references: 22138600
references: Diagn Microbiol Infect Dis. 2014 Oct;80(2):159-61
references: 25053201
references: Appl Environ Microbiol. 2009 Apr;75(7):1908-15
references: 19181840
references: J Clin Microbiol. 2010 Sep;48(9):3267-73
references: 20610688
references: J Infect Chemother. 2007 Feb;13(1):18-23
references: 17334724
references: Antimicrob Agents Chemother. 2009 Jun;53(6):2227-38
references: 19307361
references: Clin Microbiol Infect. 2010 May;16(5):472-6
references: 19523051
references: J Med Microbiol. 2008 Oct;57(Pt 10):1308-1310
references: 18809566