title: Developmental and endocrine regulation of kisspeptin expression in mouse Leydig cells.
abstract: Kisspeptin, encoded by the Kiss1 gene, binds to a specific G protein-coupled receptor (kisspeptin1 receptor) to regulate the central reproductive axis. Kisspeptin has also been reported to be expressed in peripheral tissues, including the testes. However, factors regulating testicular kisspeptin and its role in reproduction are unknown. Our objective herein was to begin to address kisspeptin function in the testis. In particular, we sought to determine the level of kisspeptin in the testis in comparison with the brain and other tissues, how these levels change from the prepubertal period through sexual maturation, and the factors involved in kisspeptin regulation in the testis. Immunohistochemical analysis of testis sections using a validated kisspeptin antibody localized kisspeptin to the Leydig cells. Kisspeptin was not detected in germ cells or Sertoli cells within the seminiferous tubules at any developmental time period studied, from prepuberty to sexual maturation. A developmental time course of testicular kisspeptin revealed that its mRNA and protein levels increased during development, reaching robust levels at postnatal day 28, correlating with pubertal onset. In vitro studies of primary mouse Leydig cells, as well as in vivo studies, indicated clearly that LH is involved in regulating levels of Leydig cell kisspeptin. Interestingly, gonadectomy resulted in elevated LH but reduced serum kisspeptin levels, suggesting that testicular kisspeptin may be secreted. These data document kisspeptin expression in mouse Leydig cells, its secretion into peripheral serum, and its regulation by changes in reproductive neuroendocrine function.
authors: Divisions of Endocrinology (S.S., I.A., A.W., S.R.) and Metabolism (B.A.H., F.W.), Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287; and Department of Biochemistry and Molecular Biology (H.C., B.R.Z.), Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205.
authors: Sajad
authors: S
authors: Salehi
authors: Ikeoluwa
authors: I
authors: Adeshina
authors: Haolin
authors: H
authors: Chen
authors: Barry R
authors: BR
authors: Zirkin
authors: Mehboob A
authors: MA
authors: Hussain
authors: Fredric
authors: F
authors: Wondisford
authors: Andrew
authors: A
authors: Wolfe
authors: Sally
authors: S
authors: Radovick
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism*; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D051379:Mice; D012708:Sertoli Cells / Q000378:metabolism; D012741:Sexual Maturation / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
chemical_list: C491407:Kiss1 protein, mouse; D059648:Kisspeptins; D007986:Luteinizing Hormone
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