title: Role of microRNAs -29b-2, -155, -197 and -205 as diagnostic biomarkers in serum of breast cancer females.
abstract: Micro-RNAs (miRs) are known to be differentially expressed in the serum of cancer patients and controls, and can thus be used as biomarkers for cancer screening. We detected the expression level of miR-29b-2, 155, 197 and 205 in the serum of female breast cancer patients and healthy controls to detect whether serum level of this chosen micro-RNAs could detect patients with breast cancer and also to detect difference in level of micro-RNAs between non-metastatic cases and metastatic cases, also we tried to detect any relation between level of micro-RNAs and the stage of the tumor, the size of tumor, nodal affection, the presence of metastasis and also the site of metastasis. Serum samples were collected from 130 female patients, 80 with non-metastatic breast cancer, 20 with metastatic breast cancer and 30 healthy controls. Real-time quantitative PCR was used to detect the expression level of miR-29b-2, -155, -197 and -205. The expression level of miR-29b-2, -155, -197 and -205 was significantly increased in the serum of breast cancer patients. miR-29b-2, -155, -197 and -205 may be useful as a blood-based biomarker for breast cancer screening.
authors: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Electronic address: olfatshaker@yahoo.com.
authors: Olfat
authors: O
authors: Shaker
authors: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
authors: Moataz
authors: M
authors: Maher
authors: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
authors: Yasser
authors: Y
authors: Nassar
authors: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
authors: George
authors: G
authors: Morcos
authors: Surgical Oncology, National Cancer Institute, Cairo University, Egypt.
authors: Ziad
authors: Z
authors: Gad
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C524942:MIRN155 microRNA, human; C546085:MIRN197 microRNA, human; C534425:MIR205, human; C532045:MIRN29a microRNA, human
keywords: Breast cancer; Metastasis; miR-155; miR-197; miR-205; miR-29b-2