title: Does Chemotherapy Change Expression of VEGF A&C and MVD in Acute Myeloid Leukemia?
abstract: BACKGROUND
  Acute Myeloid Leukemia is a malignant transformation of hematopoietic tissue, bone marrow infiltration of undifferentiated cells known as blasts that interfere with the production of normal cells. Vascular endothelial growth factor (VEGF) is persistently secreted from myeloid cells and high levels can be detected in patients' serum.
  
  
  METHODS
  Twenty-one AML patients, who were chemotherapy candidates were evaluated in a clinical trial. Serum VEGF was measured by ELISA. VEGFA, VEGFC mRNA and bone marrow MVD were measured in all patients before and after chemotherapy and then all results were analyzed.
  
  
  RESULTS
  There were 10 (48%) female and 11(52%) male patients ranged in age from 20 to 60 years, with an average age of 39.5 ±14.1 years. The mean amount of MVD was reduced from 10.8±3.6 before chemotherapy to7.6±3.3 after chemotherapy (P=0.008). VEGF was also reduced from 0.59±0.16 before chemotherapy to 0.24±0.03 after chemotherapy (P=0.005). Gene expression differences for VEGFA mRNA was 4.6±1.4, while it was 120.7±93.2 for VEGFC mRNA, showing the significance only for VEGA mRNA (P=0.02).
  
  
  CONCLUSIONS
  Regarding reduced angiogenesis, we can conclude that anti-angiogenic preparations can be effective in treatment course of AML in combination with chemotherapy regimen.
authors: Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz ,Iran.
authors: Zohreh
authors: Z
authors: Sanaat
authors: Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz ,Iran.
authors: Reza
authors: R
authors: Khalili
authors: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Shohreh
authors: S
authors: Almasi
authors: Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz ,Iran.
authors: Mohammad Reza
authors: MR
authors: Aliparasti
authors: Pathology department, shariati hospital, Tehran University of Medical Science, Tehran, Iran.
authors: Seyed-Mohammad
authors: SM
authors: Tavangar
authors: Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz ,Iran.
authors: Aliakbar
authors: A
authors: Movasaghpoor
authors: Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz ,Iran.
authors: Fariba
authors: F
authors: Kazemi
authors: Basic research team, KUMC Cardiovascular Research Institute, Kansas city, Kansas, USA.
authors: Arash
authors: A
authors: Davani
keywords: AML; Angiogenesis; MVD; VEGF
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