title: Ischemic postconditioning enhances glycogen synthase kinase-3β expression and alleviates cerebral ischemia/reperfusion injury.
abstract: The present study established global brain ischemia using the four-vessel occlusion method. Following three rounds of reperfusion for 30 seconds, and occlusion for 10 seconds, followed by reperfusion for 48 hours, infarct area, the number of TUNEL-positive cells and Bcl-2 expression were significantly reduced. However, glycogen synthase kinase-3β activity, cortical Bax and caspase-3 expression significantly increased, similar to results following ischemic postconditioning. Our results indicated that ischemic postconditioning may enhance glycogen synthase kinase-3β activity, a downstream molecule of the phosphatase and tensin homolog deleted on chromosome 10/phosphatidylinositol 3-kinase/protein kinase B signaling pathway, which reduces caspase-3 expression to protect the brain against ischemic injury.
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors: Bo
authors: B
authors: Zhao
authors: Department of Intensive Care Unit, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors: Wenwei
authors: W
authors: Gao
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors: Jiabao
authors: J
authors: Hou
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors: Yang
authors: Y
authors: Wu
authors: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors: Zhongyuan
authors: Z
authors: Xia
keywords: apoptosis; cerebral ischemia/reperfusion; glycogen synthase kinase-3β; ischemic postconditioning; ischemic preconditioning; neural regeneration
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