title: Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio).
abstract: The effect of dietary Echinacea purpurea (EP) on the response of zebrafish (Danio rerio) to a Flavobacterium columnare vaccine was investigated. Two hundred D. rerio with an average weight of 290 ± 40 g were selected and fed different levels of E. purpurea (5 g kg(-1) diet--group 1, 10 g kg(-1) diet--group 2, 20 g kg(-1) diet--group 3, 30 g kg(-1) diet--group 4, and 0 g kg(-1) diet--group 5). Experimental feeding was begun 3 weeks prior to bath immunization and continued until the end of the experiment. Twenty-eight days after immunization the fish were challenged by bath immersion with F. columnare at a concentration of 1 x 10(6) CFU/ml. The relative percent survival of the experimental groups (1, 2, 3, 4 and 5) was 5.0, 6.0, 30.0, 36.0 and 5.0, respectively. In conclusion, diet supplementation with E. purpurea may effectively enhance the response of zebrafish to a F. columnare vaccine.
authors: L
authors: L
authors: Guz
authors: K
authors: K
authors: Puk
authors: N
authors: N
authors: Walczak
authors: T
authors: T
authors: Oniszczuk
authors: A
authors: A
authors: Oniszczuk
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D020900:Echinacea / Q000737:chemistry*; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control; D045826:Flavobacteriaceae Infections / Q000517:prevention & control*; D005417:Flavobacterium / Q000276:immunology*; D015027:Zebrafish
chemical_list: D001428:Bacterial Vaccines