title: Single muscle fiber proteomics reveals unexpected mitochondrial specialization.
abstract: Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.
authors: Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Biomedical Sciences, University of Padova, Padua, Italy mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.
authors: Marta
authors: M
authors: Murgia
authors: Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
authors: Nagarjuna
authors: N
authors: Nagaraj
authors: Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
authors: Atul S
authors: AS
authors: Deshmukh
authors: Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
authors: Marlis
authors: M
authors: Zeiler
authors: Department of Biomedical Sciences, University of Padova, Padua, Italy.
authors: Pasqua
authors: P
authors: Cancellara
authors: Venetian Institute of Molecular Medicine, Padua, Italy.
authors: Irene
authors: I
authors: Moretti
authors: Department of Biomedical Sciences, University of Padova, Padua, Italy.
authors: Carlo
authors: C
authors: Reggiani
authors: Venetian Institute of Molecular Medicine, Padua, Italy mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.
authors: Stefano
authors: S
authors: Schiaffino
authors: Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.
authors: Matthias
authors: M
authors: Mann
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology / Q000379:methods; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: exercise; metabolism; mitochondria; muscle fibers; single cell
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