title: Analysis of mitochondrial morphology and function under conditions of mitofusin 2 deficiency.
abstract: Recent discoveries linking mitochondrial dynamics to various pathologies have generated interest in the design of robust methods to screen proteins involved in mitochondrial dynamics and small molecules that modulate mitochondrial dynamics. Here, we describe functional screening protocols to analyze mitochondrial parameters such as mitochondrial morphology, reactive oxygen species (ROS) levels, mitochondrial calcium, and oxygen consumption rate in cultured cells. Novel proteins that participate in the regulation of mitochondrial dynamics and function can be identified using such an approach.
authors: Institute for Research in Biomedicine (IRB Barcelona), C/Baldiri Reixac 10, 08028, Barcelona, Spain.
authors: Juan Pablo
authors: JP
authors: Muñoz
authors: Antonio
authors: A
authors: Zorzano
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D005434:Flow Cytometry; D020558:GTP Phosphohydrolases / Q000172:deficiency* / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000172:deficiency* / Q000235:genetics; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; D020558:GTP Phosphohydrolases; C470018:MFN2 protein, human; C488751:Mfn2 protein, mouse; D002118:Calcium