title: Elevated levels of CYP94 family gene expression alleviate the jasmonate response and enhance salt tolerance in rice.
abstract: The plant hormone jasmonate and its conjugates (JAs) have important roles in growth control, leaf senescence and defense responses against insects and microbial attacks. JA biosynthesis is induced by several stresses, including mechanical wounding, pathogen attacks, drought and salinity stresses. However, the roles of JAs under abiotic stress conditions are unclear. Here we report that increased expression of the Cyt P450 family gene CYP94C2b enhanced viability of rice plants under saline conditions. This gene encodes an enzyme closely related to CYP94C1 that catalyzes conversion of bioactive jasmonate-isoleucine (JA-Ile) into 12OH-JA-Ile and 12COOH-JA-Ile. Inactivation of JA was facilitated in a rice line with enhanced CYP94C2b expression, and responses to exogenous JA and wounding were alleviated. Moreover, salt stress-induced leaf senescence but not natural senescence was delayed in the transgenic rice. These results suggest that bioactive JAs have a negative effect on viability under salt stress conditions and demonstrate that manipulating JA metabolism confers enhanced salt tolerance in rice.
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Ken-ichi
authors: K
authors: Kurotani
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Kenji
authors: K
authors: Hayashi
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Saki
authors: S
authors: Hatanaka
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Yosuke
authors: Y
authors: Toda
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Daisuke
authors: D
authors: Ogawa
authors: National Institute of Agrobiological Sciences, Kannondai, Tsukuba, 305-8602 Japan.
authors: Hiroaki
authors: H
authors: Ichikawa
authors: Department of Chemistry, Tohoku University, Sendai, 980-8578 Japan.
authors: Yasuhiro
authors: Y
authors: Ishimaru
authors: Department of Chemistry, Tohoku University, Sendai, 980-8578 Japan.
authors: Ryo
authors: R
authors: Tashita
authors: Department of Chemistry, Tohoku University, Sendai, 980-8578 Japan.
authors: Takeshi
authors: T
authors: Suzuki
authors: Department of Chemistry, Tohoku University, Sendai, 980-8578 Japan.
authors: Minoru
authors: M
authors: Ueda
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan.
authors: Tsukaho
authors: T
authors: Hattori
authors: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan takeda@agr.nagoya-u.ac.jp.
authors: Shin
authors: S
authors: Takeda
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D005810:Multigene Family; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D012965:Sodium Chloride; C011006:jasmonic acid; D003577:Cytochrome P-450 Enzyme System
keywords: Cytochrome P450; Jasmonate; Rice; Salt tolerance; Senescence