title: Ozone affects growth and development of Pieris brassicae on the wild host plant Brassica nigra.
abstract: When plants are exposed to ozone they exhibit changes in both primary and secondary metabolism, which may affect their interactions with herbivorous insects. Here we investigated the performance and preferences of the specialist herbivore Pieris brassicae on the wild plant Brassica nigra under elevated ozone conditions. The direct and indirect effects of ozone on the plant-herbivore system were studied. In both cases ozone exposure had a negative effect on P. brassicae development. However, in dual-choice tests larvae preferentially consumed plant material previously fumigated with the highest concentration tested, showing a lack of correlation between larval preference and performance on ozone exposed plants. Metabolomic analysis of leaf material subjected to combinations of ozone and herbivore-feeding, and focussing on known defence metabolites, indicated that P. brassicae behaviour and performance were associated with ozone-induced alterations to glucosinolate and phenolic pools.
authors: Department of Environmental Science, University of Eastern Finland, P.O.Box 1627, FIN-70211, Kuopio, Finland. Electronic address: eliezer.khaling@uef.fi.
authors: Eliezer
authors: E
authors: Khaling
authors: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187, Umeå, Sweden.
authors: Stefano
authors: S
authors: Papazian
authors: Laboratory of Entomology, Wageningen University, P.O. Box 8031, NL-6700, EH, Wageningen, The Netherlands.
authors: Erik H
authors: EH
authors: Poelman
authors: Department of Environmental Science, University of Eastern Finland, P.O.Box 1627, FIN-70211, Kuopio, Finland.
authors: Jarmo K
authors: JK
authors: Holopainen
authors: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187, Umeå, Sweden; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK 1871, Frederiksberg C, Denmark.
authors: Benedicte R
authors: BR
authors: Albrectsen
authors: Department of Environmental Science, University of Eastern Finland, P.O.Box 1627, FIN-70211, Kuopio, Finland.
authors: James D
authors: JD
authors: Blande
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001937:Brassica / Q000187:drug effects / Q000378:metabolism; D002080:Butterflies / Q000254:growth & development / Q000502:physiology*; D005961:Glucosinolates / Q000378:metabolism; D060434:Herbivory; D007814:Larva / Q000254:growth & development; D009149:Mustard Plant; D010126:Ozone / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects; D013312:Stress, Physiological
chemical_list: D000393:Air Pollutants; D005961:Glucosinolates; D010126:Ozone
keywords: Brassica nigra; Defence compounds; Glucosinolates; Growth and performance; Ozone exposure; Phenolics; Pieris brassicae