title: Comparison of multiplex immunochemical and molecular serotyping methods for Shiga toxin-producing Escherichia coli.
abstract: Traditionally, serotyping of Escherichia coli has been performed via slide agglutination methods using antisera. More recently, multiplex immunoassays and "molecular serotyping" via polymerase chain reaction (PCR) have been validated for this purpose. In this study, the serogroups of 161 Shiga toxin-producing Escherichia coli (STEC) strains isolated from fecal samples of California cattle were typed by conventional methods using antisera as well as two newly developed multiplex PCR- and antibody-based microbead assays using the Luminex technology. Using the Luminex assays, we were capable of serotyping 11 STEC isolates that were previously determined untypeable for the O antigen by conventional methods using antisera. Except for 14 isolates, results from the 2 Luminex assays agreed.
authors: 1 MagArray , Milpitas, California.
authors: Laurie M
authors: LM
authors: Clotilde
authors: Alexandra
authors: A
authors: Salvador
authors: Clay
authors: C
authors: Bernard
authors: Andrew
authors: A
authors: Lin
authors: Carol
authors: C
authors: Lauzon
authors: Mark
authors: M
authors: Muldoon
authors: Yichun
authors: Y
authors: Xu
authors: John Mark
authors: JM
authors: Carter
mesh_terms: D000818:Animals; D002140:California; D002417:Cattle; D029968:Escherichia coli Proteins / Q000032:analysis; D005243:Feces / Q000382:microbiology; D016231:Fluorescence Polarization Immunoassay; D008863:Microspheres; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D019081:O Antigens / Q000032:analysis; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D019081:O Antigens