title: Acute response of peripheral CCr5 chemoreceptor and NK cells in individuals submitted to a single session of low-intensity strength exercise with blood flow restriction.
abstract: The purpose of this study was to compare the peripheral expression of natural killers and CCR5 in a session of low-intensity strength training with vascular occlusion and in high-intensity training. Young males were randomized into session groups of a high-intensity strength training (HI) and a session group of low-intensity strength training with vascular occlusion (LI-BFR). The exercise session consisted in knee extension and bicep curl in 80% 1RM (HI) and 30% 1RM (LI-BFR) with equalized volumes. Blood collection was made before, immediately after and 24 h after each training session. Immunophenotyping was carried out through CD195+ (CCR5) e CD3-CD16+CD56+ (NK) in peripheral blood and analysed by flow cytometry and presented in frequency (%). Peripheral frequency of NK cells showed no significant difference in LI-BFR group in time effect, while a gradual reduction of NK cells was identified in HI group in before-24 h postexercise and after-24 h postexercise comparison. However, significant differences have been found in relative change of NK cells immediately after exercise between sessions. In addition, HI and LI-BFR groups showed a significant reduction in the cells expressed CCR5 during 24 h postsession compared to the postsession, but CCR5 also differed when comparing before-24 h after session in the HI group. No differences were observed amongst the groups. LIO induced CCR5 response similar to the HI session, while the NK cells remained in similar frequency during the studied moments in LI-BFR, but not in HI group, suggesting that local hypoxia created by the blood flow restriction was able to prevent a change in the frequency of peripheral cells and a possible immunosuppression.
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.
authors: Gilson Pires
authors: GP
authors: Dorneles
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.
authors: Alana Schraiber
authors: AS
authors: Colato
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.
authors: Simone Lunelli
authors: SL
authors: Galvão
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.
authors: Thiago Rozales
authors: TR
authors: Ramis
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.
authors: Jerri Luiz
authors: JL
authors: Ribeiro
authors: Department of Basic Health Sciences, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Brazil.
authors: Pedro Roosevelt
authors: PR
authors: Romão
authors: Laboratory of Immunology and Exercise Physiology, Centro Universitário Metodista IPA, Porto Alegre, Brazil.|Department of Basic Health Sciences, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Brazil.
authors: Alessandra
authors: A
authors: Peres
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015536:Down-Regulation; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D016130:Immunophenotyping / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D009119:Muscle Contraction; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000098:blood supply*; D010641:Phenotype; D011897:Random Allocation; D019713:Receptors, CCR5 / Q000097:blood*; D012039:Regional Blood Flow; D055070:Resistance Training / Q000379:methods*; D013997:Time Factors; D014111:Tourniquets; D055815:Young Adult
chemical_list: D015415:Biomarkers; C584933:CCR5 protein, human; D019713:Receptors, CCR5
keywords: chemoreceptor; immune; inflammation; leucocytes; natural killer cells; resistance exercise; vascular occlusion