title: Identification of reference genes and validation for gene expression studies in diverse axolotl (Ambystoma mexicanum) tissues.
abstract: For the precise quantitative RT-PCR normalization a set of valid reference genes is obligatory. Moreover have to be taken into concern the experimental conditions as they bias the regulation of reference genes. Up till now, no reference targets have been described for the axolotl (Ambystoma mexicanum). In a search in the public database SalSite for genetic information of the axolotl we identified fourteen presumptive reference genes, eleven of which were further tested for their gene expression stability. This study characterizes the expressional patterns of 11 putative endogenous control genes during axolotl limb regeneration and in an axolotl tissue panel. All 11 reference genes showed variable expression. Strikingly, ACTB was to be found most stable expressed in all comparative tissue groups, so we reason it to be suitable for all different kinds of axolotl tissue-type investigations. Moreover do we suggest GAPDH and RPLP0 as suitable for certain axolotl tissue analysis. When it comes to axolotl limb regeneration, a validated pair of reference genes is ODC and RPLP0. With these findings, new insights into axolotl gene expression profiling might be gained.
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Eileen
authors: E
authors: Guelke
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Vesna
authors: V
authors: Bucan
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Christina
authors: C
authors: Liebsch
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Andrea
authors: A
authors: Lazaridis
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Christine
authors: C
authors: Radtke
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
authors: Peter M
authors: PM
authors: Vogt
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany. Electronic address: reimers.kerstin@mh-hannover.de.
authors: Kerstin
authors: K
authors: Reimers
mesh_terms: D000558:Ambystoma mexicanum / Q000235:genetics* / Q000502:physiology; D000818:Animals; D005121:Extremities / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D020043:Genes, Essential; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D009955:Ornithine Decarboxylase / Q000235:genetics; D020871:RNA Stability; D012015:Reference Standards; D012038:Regeneration / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*; D012269:Ribosomal Proteins / Q000235:genetics; D054928:Validation Studies as Topic
chemical_list: D012269:Ribosomal Proteins; C054675:ribosomal protein P0; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009955:Ornithine Decarboxylase
keywords: ACTB; Axolotl (Ambystoma mexicanum); GAPDH; Gene expression; Limb regeneration; Normalization; Reference genes