title: Analysis of mitochondrial dysfunction during cell death.
abstract: Mitochondria play a key role in various modes of cell death. Analysis of mitochondrial dysfunction and the release of proteins from the intermembrane space of mitochondria represent essential tools in cell death investigation. Here we describe how to evaluate release of intermembrane space proteins during apoptosis, alterations in the mitochondrial membrane potential, and oxygen consumption in apoptotic cells.
authors: Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, 210, Stockholm, 171 77, Sweden, Vladimir.Gogvadze@ki.se.
authors: Vladimir
authors: V
authors: Gogvadze
authors: Sten
authors: S
authors: Orrenius
authors: Boris
authors: B
authors: Zhivotovsky
mesh_terms: D017209:Apoptosis; D016923:Cell Death; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D019069:Cell Respiration; D045304:Cytochromes c / Q000378:metabolism; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption
chemical_list: D045304:Cytochromes c; D004072:Digitonin