title: Infiltrating mast cells correlate with angiogenesis in bone metastases from gastric cancer patients.
abstract: While gastric cancer is a well established angiogenesis driven tumor, no data has been published regarding angiogenesis stimulated by mast cells (MCs) positive for tryptase in bone metastases from gastric cancer patients (BMGCP). It is well established that MCs play a role in immune responses and more recently it was demonstrated that MCs have been involved in tumor angiogenesis. We analyzed infiltrating MCs and neovascularization in BMGCP diagnosed by histology. A series of 15 stage T3-4N2-3M1 (by AJCC for Gastric Cancer Staging 7th Edition) BMGCP from bone biopsies were selected. Tumour tissue samples were evaluated by mean of immunohistochemistry and image analysis methods in terms of MCs density positive to tryptase (MCDPT), MCs area positive to tryptase (MCAPT), microvascular density (MVD) and endothelial area (EA). A significant correlation between MCDPT, MCAPT, MVD and EA groups to each other was found by Pearson and t-test analysis (r ranged from 0.68 to 0.82; p-value ranged from 0.00 to 0.02). Our very preliminary data suggest that infiltrating MCs positive for tryptase may play a role in BMGCP angiogenesis, and could be further evaluated as a novel target of anti-angiogenic therapy.
authors: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, Catanzaro 88100, Italy. eustachio.ruggieri@tiscali.it.|Surgery Unit, National Cancer Research Centre Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, Bari 70124, Italy. eustachio.ruggieri@tiscali.it.
authors: Michele
authors: M
authors: Ammendola
authors: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, Bari 70124, Italy. ilariamare@tin.it.
authors: Ilaria
authors: I
authors: Marech
authors: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, Catanzaro 88100, Italy. sammarco@unicz.it.
authors: Giuseppe
authors: G
authors: Sammarco
authors: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, Catanzaro 88100, Italy. valezy@libero.it.
authors: Valeria
authors: V
authors: Zuccalà
authors: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, Catanzaro 88100, Italy. marilyn_luposella@live.it.
authors: Maria
authors: M
authors: Luposella
authors: Chair of Pathology, Veterinary Medical School, University "Aldo Moro" of Bari, Via Casamassima, Bari 70010, Italy. nicola.zizzo@uniba.it.
authors: Nicola
authors: N
authors: Zizzo
authors: Chair of Pathology, Veterinary Medical School, University "Aldo Moro" of Bari, Via Casamassima, Bari 70010, Italy. rosavet@libero.it.
authors: Rosa
authors: R
authors: Patruno
authors: Chair of Pathology, Veterinary Medical School, University "Aldo Moro" of Bari, Via Casamassima, Bari 70010, Italy. alberto.crovace@libero.it.|Department of Emergency and Organ Transplantation (D.E.T.O.), Veterinary Medical School, Università "Aldo Moro", Via Casamassima, Bari 70010, Italy. alberto.crovace@libero.it.
authors: Alberto
authors: A
authors: Crovace
authors: Surgery Unit, National Cancer Research Centre Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, Bari 70124, Italy. eustachio.ruggieri@tiscali.it.
authors: Eustachio
authors: E
authors: Ruggieri
authors: Pathology Unit, ASL BA, Contrada Capo Scardicchio 92, Bari 70100, Italy. fazito@libero.it.
authors: Alfredo Francesco
authors: AF
authors: Zito
authors: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, Bari 70124, Italy. c.gadaleta@oncologico.bari.it.
authors: Cosmo Damiano
authors: CD
authors: Gadaleta
authors: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, Catanzaro 88100, Italy. sacco@unicz.it.
authors: Rosario
authors: R
authors: Sacco
authors: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, Bari 70124, Italy. giroran@tiscalinet.it.
authors: Girolamo
authors: G
authors: Ranieri
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001859:Bone Neoplasms / Q000473:pathology* / Q000556:secondary*; D002452:Cell Count; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000276:immunology / Q000378:metabolism; D013274:Stomach Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D047368:Tumor Burden
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