title: Yeast as a tool to study mitochondrial retrograde pathway en route to cell stress response.
abstract: Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional mitochondria relay signals that lead to cell stress adaptation in physiopathological conditions by changes in nuclear gene expression. The best comprehension of components and regulation of retrograde signaling have been obtained in Saccharomyces cerevisiae, where retrograde target gene expression is regulated by RTG genes. In this chapter, we describe the methods to measure mitochondrial retrograde pathway activation in yeast cells by monitoring the mRNA levels of RTG target genes, such as those encoding for peroxisomal citrate synthase, aconitase, and NAD(+)-specific isocitrate dehydrogenase subunit 1, as well as the phosphorylation status of Rtg1/3p transcriptional factor which controls RTG target gene transcription.
authors: CNR, Istituto di Biomembrane e Bioenergetica, Via Amendola 165/A, 70126, Bari, Italy.
authors: Maša
authors: M
authors: Ždralević
authors: Nicoletta
authors: N
authors: Guaragnella
authors: Sergio
authors: S
authors: Giannattasio
mesh_terms: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D029701:Saccharomyces cerevisiae Proteins