title: PsbS is required for systemic acquired acclimation and post-excess-light-stress optimization of chlorophyll fluorescence decay times in Arabidopsis.
abstract: Systemic acquired acclimation (SAA) is an important light acclimatory mechanism that depends on the global adjustments of non-photochemical quenching and chloroplast retrograde signaling. As the exact regulation of these processes is not known, we measured time-resolved fluorescence of chlorophyll a in Arabidopsis thaliana leaves exposed to excess light, in leaves undergoing SAA, and in leaves after excess light episode. We compare the behavior induced in wild-type plants with null mutant of non-photochemical quenching (npq4-1). The wild type rosettes exhibit a small reduction of fluorescence decay times in leaves directly exposed to excess light and in leaves undergoing SAA in ambient low light. However in npq4-1 exposition to excess light results in much faster fluorescence decay, which is insensitive to excitation power. At the same time npq4-1 leaves undergoing SAA displayed intermediate fluorescence decay. The npq4-1 plants also lost the ability to optimize florescence decay, and thus chlorophyll a dynamics up to 2 h after excess light episode. The fluorescence decay dynamics in both WT and npq4-1 can be described by a set of 3 maximum decay times. Based on the results, we concluded that functional PsbS is required for optimization of absorbed photon fate and optimal light acclimatory responses such as SAA or after excess light stress.
authors: a Institute of Physics; Faculty of Physics; Astronomy and Informatics ; Nicolaus Copernicus University ; Toruń , Poland.
authors: Kamil
authors: K
authors: Ciszak
authors: Milena
authors: M
authors: Kulasek
authors: Anna
authors: A
authors: Barczak
authors: Justyna
authors: J
authors: Grzelak
authors: Sebastian
authors: S
authors: Maćkowski
authors: Stanisław
authors: S
authors: Karpiński
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000502:physiology*; D005453:Fluorescence; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; C489731:NPQ4 protein, Arabidopsis; D045332:Photosystem II Protein Complex; D002734:Chlorophyll
keywords: DCMU, 3,4-dichlorophenyl)-1,1-dimethyl urea; EEE, Excess Excitation Energy; EL, Excess Light; F0, chlorophyll fluorescence zero; FD, chlorophyll fluorescence decay; Fm, chlorophyll fluorescence maximum; Fv, chlorophyll fluorescence variable; Fv/Fm, maximum quantum efficiency of PSII; LED, Light Emitting Diode; LHC, chlorophyll a/b/xanthophyll-binding proteins; NPQ, Non-Photochemical Quenching; PSII, Photosystem II; ROS, Reactive Oxygen Species; SAA, Systemic Acquired Acclimation; WT, Wild Type; dynamics of chlorophyll fluorescence; excess excitation energy dissipation; light acclimation; non-photochemical quenching; photosystem II; qE, EEE thermal dissipation; qI, photoinhibition; qT, state transition; qZ, zeaxanthin formation; systemic acquired acclimation
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