title: Metabolites of isocorynoxeine in rats after its oral administration.
abstract: This work presents the metabolites of isocorynoxeine (ICOR), which is one of four bioactive tetracyclic oxindole alkaloids isolated from Uncaria hooks used commonly in the traditional Chinese medicines and Kampo medicines. After oral administration of 40 mg kg(-1) ICOR to rats, bile was drained and analyzed by LC-MS. Two phase I metabolites, namely 11-hydroxyisocorynoxeine (M1) and 10-hydroxyisocorynoxeine (M2), and two phase II metabolites, namely 11-hydroxyisocorynoxeine 11-O-β-D-glucuronide (M3) and 10-hydroxyisocorynoxeine 10-O-β-D-glucuronide (M4), were isolated from rat excreta and bile, respectively, whose structures were elucidated on the basis of CD, NMR, and MS.
authors: a School of Pharmaceutical Sciences and Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University , Kunming 650500 , China.
authors: Ya-Ping
authors: YP
authors: Chen
authors: Min-Nan
authors: MN
authors: Lu
authors: Jing-Chao
authors: JC
authors: Hao
authors: Mei-Hong
authors: MH
authors: Li
authors: Masao
authors: M
authors: Hattori
authors: Wei
authors: W
authors: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid; D020719:Glucuronides / Q000737:chemistry; D026121:Indole Alkaloids / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D051381:Rats; D032070:Uncaria / Q000737:chemistry*
chemical_list: D020719:Glucuronides; D026121:Indole Alkaloids; C054799:corynoxeine
keywords: isocorynoxeine; metabolites; structure elucidation