title: Methylenetetrahydrofolate reductase C677T and A1298C polymorphism and susceptibility to acute lymphoblastic leukemia in a cohort of Egyptian children.
abstract: This case-control study was planned to investigate the possible role of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms as a risk factor for the development of acute lymphoblastic leukemia (ALL) in a cohort of Egyptian children. Typing of MTHFR C677T and A1298C polymorphisms was done using restriction fragment length polymorphism (RFLP) for 100 children with ALL and 100 age- and sex-matched healthy controls. No significant differences were found between patients with ALL and controls for the frequency of MTHFR C677T and A1298C alleles, genotypes, combined genotypes or haplotypes. The C677T and A1298C genotype frequency was different from that in Korean and Chinese populations (p < 0.5) and was similar to that in British, French-Canadian and German-Caucasian populations (p > 0.5). Our findings suggest that MTHFR C677T and A1298C polymorphisms are unlikely to affect the development of childhood ALL in an Egyptian population from Delta.
authors: a Clinical Immunology Unit, Clinical Pathology Department and Mansoura Research Center for Cord Stem Cells (MARC_CSC) , Faculty of Medicine, Mansoura University , Mansoura , Egypt.
authors: Youssef M
authors: YM
authors: Mosaad
authors: b Clinical Hematology Unit, Clinical Pathology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors: Nashwa K
authors: NK
authors: Abousamra
authors: c Hematology/Oncology Unit, Mansoura University Children's Hospital, Faculty of Medicine, Mansoura University , Mansoura , Egypt.
authors: Rasha
authors: R
authors: Elashery
authors: d Laboratory Medicine Department, Mansoura Fever Hospital , Ministry of Health , Mansoura , Egypt.
authors: Iman M
authors: IM
authors: Fawzy
authors: b Clinical Hematology Unit, Clinical Pathology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors: Omar A Sharaf
authors: OA
authors: Eldein
authors: e Medical Microbiology and Immunology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
authors: Doaa M
authors: DM
authors: Sherief
authors: f Laboratory Medicine Department, Samanoud Central Hospital , Ministry of Health , Samanoud , Egypt.
authors: Hend M M
authors: HM
authors: El Azab
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000483:Alleles; D019943:Amino Acid Substitution; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003062:Codon; D004534:Egypt; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D015810:Linkage Disequilibrium; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*
chemical_list: D003062:Codon; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: ALL; Egyptian; LD; MTHFR; RFLP; childhood