title: Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants.
abstract: Eight strains, representing two novel anamorphic yeast species, consisted of five strains isolated from the external surfaces of rice leaves (DMKU-RP72(T), DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and one from a corn leaf (DMKU-CP430(T))4 collected in Thailand, and one strain isolated from each of a composite flower (11-1114) and a fallen dead leaf (12-301); the latter two were collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, they were suggested to be two novel species of the genus Hannaella. Seven strains (DMKU-RP72(T), DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0-3 nt substitutions in the D1/D2 region and by 0-1 nt substitutions in the ITS region. In terms of pairwise sequence similarities of the D1/D2 region these seven strains were closest to Hannaella zeae, but with 1.2-1.7% (7-9) nucleotide substitutions. The sequences of the ITS region of these seven strains differed from H. zeae by 3.7-3.9% (16-17) nucleotide substitutions. Therefore, they were assigned to a single novel species and the name Hannaella siamensis sp. nov. has been proposed. The type strain is DMKU-RP72(T) ( = BCC 69493(T) = NBRC 110425(T) = CBS 13533(T)). Strain DMKU-CP430(T) represents the second novel species and was also most closely related to H. zeae, but with 1.0% (6) nucleotide substitutions in the D1/D2 region and 3.2% (14) nucleotide substitutions in the ITS region. It was assigned to the proposed novel species, Hannaella phetchabunensis sp. nov. (type strain DMKU-CP430(T) = BCC 69492(T) = NBRC 110424(T) = CBS 13386(T)).
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Rungluk
authors: R
authors: Kaewwichian
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Sasitorn
authors: S
authors: Jindamorakot
authors: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
authors: Somjit
authors: S
authors: Am-In
authors: Department of Genetics and Applied Microbiology, University of Debrecen, Debrecen 4000, Hungary.
authors: Matthias
authors: M
authors: Sipiczki
authors: Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Bangkok 10900, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Savitree
authors: S
authors: Limtong
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal