title: Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves.
abstract: BACKGROUND
  In this paper, we have studied the essential oils chemical composition of the leaves of seven Eucalyptus species developed in Tunisia. Eucalyptus leaves were picked from trees growing in different arboretums in Tunisia. Choucha and Mrifeg arboretums located in Sedjnene, region of Bizerte (Choucha: E. maideni, E. astrengens et E. cinerea; Mrifeg : E. leucoxylon), Korbous arboretums located in the region of Nabeul, North East Tunisia with sub-humid bioclimate, (E. lehmani), Souiniet-Ain Drahem arboretum located in region of Jendouba (E. sideroxylon, E. bicostata). Essential oils were individually tested against a large panel of microorganisms including Staphylococcus aureus (ATCC 6539), Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC29212), Listeria ivanovii (RBL 30), Bacillus cereus (ATCC11778).
  
  
  RESULTS
  The yield of essential oils ranged from 1.2% to 3% (w/w) for the different Eucalyptus species. All essential oils contain α-pinene, 1,8-cineol and pinocarveol-trans for all Eucalyptus species studied. The 1,8-cineol was the major compound in all species (49.07 to 83.59%). Diameter of inhibition zone of essential oils of Eucalyptus species varied from 10 to 29 mm. The largest zone of inhibition was obtained for Bacillus cereus (E. astrengens) and the lowest for Staphylococcus aureus (E. cinerea). The essential oils from E. maideni, E. astrengens, E. cinerea (arboretum of Bizerte), E. bicostata (arboretum of Aindraham) showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus.
  
  
  CONCLUSIONS
  The major constituents of Eucalyptus leaves essential oils are 1,8-cineol (49.07 to 83.59%) and α-pinene (1.27 to 26.35%). The essential oils from E. maideni, E. astrengens, E. cinerea, E. bicostata showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus, they may have potential applications in food and pharmaceutical products.
authors: Laboratoire de Biochimie des Lipides et Interactions avec les Macromolécules, Faculté des Sciences de Tunis, Université de Tunis-El-Manar, Elmanar, 2092, Tunis, Tunisia. k.sebei@yahoo.fr.
authors: Khaled
authors: K
authors: Sebei
authors: Laboratoire de Biochimie des Lipides et Interactions avec les Macromolécules, Faculté des Sciences de Tunis, Université de Tunis-El-Manar, Elmanar, 2092, Tunis, Tunisia. Faouzi.Sakouhi@fst.rnu.tn.
authors: Fawzi
authors: F
authors: Sakouhi
authors: Laboratoire de Biochimie des Lipides et Interactions avec les Macromolécules, Faculté des Sciences de Tunis, Université de Tunis-El-Manar, Elmanar, 2092, Tunis, Tunisia. wahid1bio@yahoo.fr.
authors: Wahid
authors: W
authors: Herchi
authors: Laboratoire d'Ecologie, INRGREF, B.P10, Ariana, 2080, Tunisia. khouja.medlarbi@iresa.agrinet.tn.
authors: Mohamed Larbi
authors: ML
authors: Khouja
authors: Laboratoire de Biochimie des Lipides et Interactions avec les Macromolécules, Faculté des Sciences de Tunis, Université de Tunis-El-Manar, Elmanar, 2092, Tunis, Tunisia. sboukhchina@yahoo.fr.
authors: Sadok
authors: S
authors: Boukhchina
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects; D000081008:Bicyclic Monoterpenes; D049109:Cell Proliferation / Q000187:drug effects; D003511:Cyclohexanols / Q000032:analysis; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D000077591:Eucalyptol; D005052:Eucalyptus / Q000737:chemistry* / Q000145:classification*; D008401:Gas Chromatography-Mass Spectrometry; D008087:Listeria / Q000145:classification / Q000187:drug effects; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D014416:Tunisia
chemical_list: D000900:Anti-Bacterial Agents; D000081008:Bicyclic Monoterpenes; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; C534320:6,6-dimethyl-2-methylenebicyclo(3.1.1)heptan-3-ol; C005451:alpha-pinene; D000077591:Eucalyptol
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