title: Effect of microgrooved surface topography on osteoblast maturation and protein adsorption.
abstract: Microgrooved surfaces have been used extensively to influence cell contact guidance. Guiding cell growth, extracellular matrix deposition, and mineralization is important for bone implant longevity. In this study, we investigated the osteoblast response to microgrooved metallic surfaces in serum-supplemented medium. Groove spacing was comparable with the spread osteoblast size. Focal adhesions were observed to confine to the intervening ridge/groove boundaries. Osteoblasts bridged over the grooves and were unable to conform to the concave shape of the underlying grooves. Microgrooved surfaces induced higher osteoblast proliferation and metabolic activity after 14 days in osteogenic medium compared with as-received surfaces, resulting in higher mineralization and alignment of cell-secreted collagen after 28 days. To establish whether preferential cell attachment at the ridge/groove boundaries was influenced by the adhesion proteins contained in the serum-supplemented media, fluorescently labeled fibronectin was adsorbed onto the microgrooved substrates at low concentrations, mimicking the concentrations found in blood serum. Fibronectin was found to selectively adsorb onto the ridge/groove boundaries, the osteoblast focal adhesion sites, suggesting that protein adsorption may have influenced the cell attachment pattern.
authors: Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, United Kingdom.
authors: Alba C
authors: AC
authors: De Luca
authors: Faculty of Physics and Earth Sciences, Institute for Experimental Physics I, Soft Matter Physics Division, University of Leipzig, Linnéstraße 5, 04103, Leipzig, Germany.
authors: Mareike
authors: M
authors: Zink
authors: Faculty of Physics and Earth Sciences, Institute for Experimental Physics I, Soft Matter Physics Division, University of Leipzig, Linnéstraße 5, 04103, Leipzig, Germany.
authors: Astrid
authors: A
authors: Weidt
authors: Leibniz Institute for Surface Modification (IOM), Translational Centre for Regenerative Medicine and Faculty of Physics and Earth Sciences, University of Leipzig, Permoserstraße 15, 04318, Leipzig, Germany.
authors: Stefan G
authors: SG
authors: Mayr
authors: Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, United Kingdom.
authors: Athina E
authors: AE
authors: Markaki
mesh_terms: D000327:Adsorption; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D011506:Proteins
keywords: contact guidance; fibronectin; grooved substrates; osteoblast cells; surface topography