title: Study of the rice straw biodegradation in mixed culture of Trichoderma viride and Aspergillus niger by GC-MS and FTIR.
abstract: This study was conducted to investigate the biodegradation ability of the mixed culture of Trichoderma viride and Aspergillus niger through the study of the organic matter extracted from rice straw and the lignocellulose structure by using gas chromatography-mass spectrometer (GC-MS) and Fourier transform infrared spectroscopy (FTIR). The results of the GC-MS showed that the mixed culture possessed shorter alkane (heptane) at the end of the incubation and more kinds of organic matter (except the alkanes, 29 kinds of organic matter were detected) than the pure cultures. It could be deduced that the organic matter could indicate the degradation degree of the lignocellulose to some extent. Moreover, pinene was detected in the mixed culture on days 5 and 10, which might represent the antagonistic relationship between T. viride and A. niger. The analysis of FTIR spectrums which indirectly verified the GC-MS results showed that the mixed culture possessed a better degradation of rice straw compared with the pure culture. Therefore, the methods used in this research could be considered as effective ones to investigate the lignocellulose degradation mechanism in mixed culture.
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China, cyn@hnu.edu.cn.
authors: Yaoning
authors: Y
authors: Chen
authors: Jingxia
authors: J
authors: Huang
authors: Yuanping
authors: Y
authors: Li
authors: Guangming
authors: G
authors: Zeng
authors: Jiachao
authors: J
authors: Zhang
authors: Aizhi
authors: A
authors: Huang
authors: Jie
authors: J
authors: Zhang
authors: Shuang
authors: S
authors: Ma
authors: Xuebin
authors: X
authors: Tan
authors: Wei
authors: W
authors: Xu
authors: Wei
authors: W
authors: Zhou
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism*; D001673:Biodegradation, Environmental; D046508:Culture Techniques; D003890:Desiccation; D008401:Gas Chromatography-Mass Spectrometry; D008031:Lignin / Q000378:metabolism*; D059013:Microbial Consortia; D012275:Oryza / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014242:Trichoderma / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D008031:Lignin
references: Bioresour Technol. 2010 Jul;101(14):5431-6
references: 20335024
references: Anal Chim Acta. 2013 Apr 24;774:33-43
references: 23567114
references: J Biotechnol. 2005 Mar 30;116(3):305-17
references: 15707691
references: FEMS Microbiol Ecol. 2000 Mar 1;31(3):185-194
references: 10719199
references: J Ethnopharmacol. 2003 Nov;89(1):165-9
references: 14522450
references: Bioresour Technol. 2002 May;83(1):1-11
references: 12058826
references: Appl Biochem Biotechnol. 2005 Spring;121-124:1081-99
references: 15930583
references: Mycol Res. 2006 Feb;110(Pt 2):161-8
references: 16488366
references: Toxicon. 2008 Jan;51(1):37-44
references: 18037463
references: Mycol Res. 2004 Dec;108(Pt 12):1447-57
references: 15757181
references: Appl Microbiol Biotechnol. 2014 May;98(9):4233-43
references: 24442505
references: Bioresour Technol. 2009 Sep;100(17):3948-62
references: 19362819
references: Bioresour Technol. 2012 Jun;114:437-42
references: 22483569
references: J Chem Ecol. 2007 Jan;33(1):43-57
references: 17146718
references: Bioresour Technol. 2005 Apr;96(6):673-86
references: 15588770
references: Bioresour Technol. 2014 Jan;151:306-13
references: 24262840