title: A dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and β-ether lignin dimer.
abstract: In the biorefinery using lignocellulosic biomass as feedstock, pretreatment to breakdown or loosen lignin is important step and various approaches have been conducted. For biological pretreatment, we screened Bacillus subtilis KCTC2023 as a potential lignin-degrading bacterium based on veratryl alcohol (VA) oxidation test and the putative heme-containing dye-decolorizing peroxidase was found in the genome of B. subtilis KCTC2023. The peroxidase from B. subtilis KCTC2023 (BsDyP) was capable of oxidizing various substrates and atypically exhibits substrate-dependent optimum temperature: 30°C for dyes (Reactive Blue19 and Reactive Black5) and 50°C for high redox potential substrates (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], VA, and veratryl glycerol-β-guaiacyl ether [VGE]) over +1.0 V vs. normal hydrogen electrode. At 50°C, optimum temperature for high redox potential substrates, BsDyP not only showed the highest VA oxidation activity (0.13 Umg(-1)) among the previously reported bacterial peroxidases but also successfully achieved VGE decomposition by cleaving Cα-Cβ bond in the absence of any oxidative mediator with a specific activity of 0.086 Umg(-1) and a conversion rate of 53.5%. Based on our results, BsDyP was identified as the first bacterial peroxidase capable of oxidizing high redox potential lignin-related model compounds, especially VGE, revealing a previously unknown versatility of lignin degrading biocatalyst in nature.
authors: Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors: Kyoungseon
authors: K
authors: Min
authors: 1] Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea [2] Interdisciplinary program of Bioengineering, Seoul National University, Seoul 151-741, Republic of Korea.
authors: Gyeongtaek
authors: G
authors: Gong
authors: Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors: Han Min
authors: HM
authors: Woo
authors: Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors: Yunje
authors: Y
authors: Kim
authors: Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors: Youngsoon
authors: Y
authors: Um
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001592:Benzyl Alcohols; D004396:Coloring Agents; D011994:Recombinant Proteins; D008031:Lignin; D009195:Peroxidase; C042197:veratryl alcohol
references: Chem Biol. 2002 May;9(5):555-65
references: 12031662
references: Biotechnol Prog. 2004 Sep-Oct;20(5):1588-92
references: 15458349
references: FEBS Lett. 2012 Dec 14;586(24):4351-6
references: 23159941
references: Bioresour Technol. 2010 Dec;101(23):8915-22
references: 20667714
references: FEBS J. 2011 Jul;278(13):2387-94
references: 21569205
references: Appl Microbiol Biotechnol. 2010 May;86(5):1419-30
references: 19967355
references: Enzyme Microb Technol. 2014 Jul-Aug;61-62:48-54
references: 24910336
references: Bioresour Technol. 2010 Oct;101(19):7592-7
references: 20537891
references: Appl Environ Microbiol. 2009 Dec;75(23):7509-18
references: 19801472
references: Appl Environ Microbiol. 1999 Mar;65(3):1029-35
references: 10049859
references: J Biol Chem. 2012 Nov 30;287(49):41053-67
references: 23071108
references: Bioresour Technol. 2014;152:429-36
references: 24316485
references: Appl Environ Microbiol. 2013 Jul;79(14):4316-24
references: 23666335
references: Enzyme Microb Technol. 2001 May 7;28(7-8):602-605
references: 11339941
references: Biotechnol Adv. 2011 Nov-Dec;29(6):675-85
references: 21624451
references: Appl Biochem Biotechnol. 2009 Jan;152(1):6-14
references: 18506630
references: Biochim Biophys Acta. 1998 Mar 2;1379(3):381-90
references: 9545600
references: ACS Chem Biol. 2012 Dec 21;7(12):2074-81
references: 23054399
references: Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16084-9
references: 19805263
references: Biochemistry. 2011 Jun 14;50(23):5096-107
references: 21534568
references: Biodegradation. 2011 Sep;22(5):1017-27
references: 21350882
references: Environ Technol. 2012 Dec;33(22-24):2603-9
references: 23437660
references: Appl Microbiol Biotechnol. 2013 Jun;97(12):5627-34
references: 23624707
references: Curr Opin Biotechnol. 2011 Jun;22(3):394-400
references: 21071202
references: Biochemistry. 1999 Nov 23;38(47):15548-55
references: 10569938
references: Appl Microbiol Biotechnol. 2014 Mar;98(5):2053-65
references: 23820555
references: Mol Biosyst. 2010 May;6(5):815-21
references: 20567767
references: BMC Biotechnol. 2011;11:94
references: 21995752
references: FEBS Lett. 1998 May 29;428(3):141-6
references: 9654123
references: J Exp Bot. 2009;60(2):441-52
references: 18987391