title: Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling.
abstract: A root canal sealer with antibacterial activity can be efficacious in preventing reinfection that results from residual microorganisms and/or the leakage of microorganisms. In the present study, a series of injectable, self-curing polyurethane (PU)-based antibacterial sealers with different concentrations of silver phosphate (Ag3PO4) were fabricated. Subsequently, their physicochemical properties, antibacterial abilities, and preliminary cytocompatibilities were evaluated. The results indicated that the fabricated PU-based sealers can achieve a high conversion rate in a short amount of time. More than 95% of the isocyanate group of PU sealers with 3 wt% (PU3) and 5 wt% (PU5) concentrations of Ag3PO4 were included in the curing reaction after 7 hours. With the exception of those for film thickness for PU5, the results of setting time, film thickness, and solubility were able to meet the requirements of the International Organization for Standardization. The antibacterial tests showed that PU3 and PU5 exhibit stronger antimicrobial effects than that achieved with 1 wt% Ag3PO4 (PU1) and AH Plus (positive control) against Streptococcus mutans. The cytocompatibility evaluation revealed that the PU1 and PU3 sealers possess good cytocompatibility and low cytotoxicity. These results demonstrate that the PU3 sealer offers good physicochemical and antimicrobial properties along with cytocompatibility, which may hold great application potential in the field of root canal fillings.
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Jian
authors: J
authors: Wang
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yi
authors: Y
authors: Zuo
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Minghui
authors: M
authors: Zhao
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Jiaxing
authors: J
authors: Jiang
authors: College of Stomatology, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yi
authors: Y
authors: Man
authors: Department of Orthopedics, Chongqing Medical University, Chongqing, People's Republic of China.
authors: Jun
authors: J
authors: Wu
authors: Department of Orthopedics, Chongqing Medical University, Chongqing, People's Republic of China.
authors: Yunjiu
authors: Y
authors: Hu
authors: College of Chemistry, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Changlei
authors: C
authors: Liu
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yubao
authors: Y
authors: Li
authors: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Jidong
authors: J
authors: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003786:Dental Pulp Cavity / Q000382:microbiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000187:drug effects*; D015035:Zinc Oxide-Eugenol Cement / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D011140:Polyurethanes; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C112894:canals sealer
keywords: antibacterial properties; direct contact test; polyurethane; root canal sealer; silver phosphate
references: J Oral Rehabil. 2004 Apr;31(4):364-7
references: 15089943
references: Int Endod J. 2005 Jul;38(7):483-8
references: 15946270
references: Int Endod J. 2003 Jul;36(7):453-63
references: 12823700
references: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999 Aug;88(2):221-5
references: 10468467
references: Endod Dent Traumatol. 1996 Aug;12(4):179-84
references: 9028181
references: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Dec;94(6):658-66
references: 12464887
references: Biochemistry. 2005 Oct 4;44(39):13214-23
references: 16185089
references: J Endod. 2008 Feb;34(2):186-9
references: 18215678
references: Eur J Oral Sci. 2004 Jun;112(3):207-15
references: 15154917
references: Biomaterials. 2004 Aug;25(18):4383-91
references: 15046929
references: J Oral Rehabil. 1994 Jul;21(4):441-52
references: 7965355
references: J Oral Sci. 2004 Sep;46(3):185-9
references: 15508752
references: J Endod. 2005 Nov;31(11):809-13
references: 16249724
references: Environ Sci Technol. 2011 Oct 15;45(20):9003-8
references: 21950450
references: Acta Biomater. 2010 Aug;6(8):3199-207
references: 20197122
references: Mater Sci Eng C Mater Biol Appl. 2013 Aug 1;33(6):3138-45
references: 23706193
references: Met Based Drugs. 1994;1(5-6):467-82
references: 18476264
references: J Biomed Mater Res. 2000 Dec 15;52(4):662-8
references: 11033548
references: J Endod. 2008 Mar;34(3):303-5
references: 18291281
references: Biomaterials. 2005 May;26(14):2081-8
references: 15576182
references: J Endod. 2002 Nov;28(11):779-83
references: 12470024
references: J Dent. 2006 Jan;34(1):35-40
references: 15907357
references: Int J Prosthodont. 2003 Mar-Apr;16(2):189-93
references: 12737253
references: Dent Mater. 2013 Mar;29(3):e29-34
references: 23107192
references: J Endod. 2004 Jan;30(1):57-60
references: 14760911
references: Int Endod J. 2008 Jan;41(1):72-7
references: 17931390
references: J Dent. 2003 May;31(4):275-81
references: 12735922
references: Clin Oral Investig. 2008 Mar;12(1):1-8
references: 18040729
references: J Prosthet Dent. 2005 Apr;93(4):364-71
references: 15798687
references: J Endod. 2000 Apr;26(4):210-6
references: 11199720
references: Oper Dent. 2000 Jul-Aug;25(4):265-9
references: 11203829