title: Internal extracellular bacteria of Diaphorina citri Kuwayama (Hemiptera: Psyllidae), the Asian citrus psyllid.
abstract: The Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an invasive insect pest that transmits Candidatus Liberibacter spp. This insect/pathogen system was first identified in North America in the early 2000's and has become the top threat to the citrus industry. Limited options for management of this problem exist; therefore, innovative pest management strategies are being developed. In this study, we describe the first step toward a paratransgenic approach (also referred to symbiotic control) for control of the insect vector or the pathogen. Culturable bacteria from the gut of Asian Citrus Psyllids were identified using standard culture techniques followed by sequencing of the cultured microorganisms. Further, 454 pyrosequencing of the gut was performed to audit bacterial presence in order to begin to identify any relationship between psyllid symbionts and C. Liberibacter spp.
authors: Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd., Hayward, CA, 94543, USA.
authors: Lakshmi D
authors: LD
authors: Kolora
authors: Christopher M
authors: CM
authors: Powell
authors: W
authors: W
authors: Hunter
authors: B
authors: B
authors: Bextine
authors: C R
authors: CR
authors: Lauzon
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D058448:Biota; D002957:Citrus / Q000469:parasitology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D006430:Hemiptera / Q000382:microbiology*; D009656:North America; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
references: Appl Environ Microbiol. 2008 Aug;74(16):4985-96
references: 18539818
references: J Appl Microbiol. 2002;92(4):759-63
references: 11966918
references: Appl Environ Microbiol. 2002 Oct;68(10):4906-14
references: 12324338
references: Environ Entomol. 2012 Feb;41(1):98-107
references: 22525064
references: Trends Microbiol. 2004 Aug;12(8):386-93
references: 15276615
references: Curr Microbiol. 2014 Oct;69(4):580-5
references: 24934994
references: Int J Parasitol. 2007 May;37(6):595-603
references: 17224154
references: PLoS Pathog. 2012;8(5):e1002742
references: 22693451
references: FEMS Microbiol Ecol. 2004 Jun 1;48(3):437-46
references: 19712312
references: Appl Environ Microbiol. 2007 Dec;73(23):7531-5
references: 17933921
references: BMC Microbiol. 2008 Jul 24;8:125
references: 18652685
references: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references: 17586664
references: Appl Environ Microbiol. 2000 Jun;66(6):2578-88
references: 10831441
references: Curr Microbiol. 2004 May;48(5):327-31
references: 15060727
references: PLoS One. 2008 Oct 03;3(10):e3326
references: 18833331
references: J Econ Entomol. 2010 Oct;103(5):1531-41
references: 21061950
references: Methods Mol Biol. 2004;268:491-502
references: 15156063
references: J Microbiol. 2005 Feb;43 Spec No:93-100
references: 15765062
references: Appl Environ Microbiol. 2010 Jun;76(11):3427-36
references: 20382817
references: Foodborne Pathog Dis. 2008 Aug;5(4):459-72
references: 18713063
references: Appl Environ Microbiol. 2009 Mar;75(6):1566-74
references: 19151177
references: J Econ Entomol. 2011 Oct;104(5):1486-95
references: 22066176
references: Appl Environ Microbiol. 2000 Jul;66(7):2898-905
references: 10877784
references: Environ Entomol. 2013 Oct;42(5):868-73
references: 24331599
references: Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12734-9
references: 22802646
references: Environ Microbiol. 2011 Jul;13(7):1889-900
references: 21631690
references: Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
references: 20534432
references: Open Microbiol J. 2012;6:29-33
references: 22529882
references: J Clin Microbiol. 2006 Nov;44(11):3980-8
references: 16988018