title: The Effect of Anterior Stromal Puncture Using Q-Switched Nd:YAG Laser on Corneal Wound Healing.
abstract: BACKGROUND
  Recurrent corneal erosion occurs when the wounded corneal epithelium failed to adhere to the underlying stroma. Therefore, this work aimed to assess the effect of treatment of corneal injury using Q- switched Nd:YAG laser.
  
  
  METHODS
  Twenty one New Zealand male rabbits weighing 2-2.5 kg and 3 months old were classified into three main groups. The control group: did not received any treatment (n=3 rabbits). The rest of the animals (n= 18 rabbits), corneal epithelium was injured by syringe needle and blade 15 and divided into:(A) Normal healing group: which was divided into three subgroups (n=3 rabbits each), and the animals were left for normal healing for1 day, 1 week, and 4 weeks respectively, (B) Laser treated group: divided into three subgroups (n=3 rabbit seach) and subjected to anterior stromal puncture using Q-switched Nd: YAG laser on corneal sub-epithelium or superficial stroma, and the animals were left for 1 day, 1 week, and 4 weeksrespectively. After the demonstrated periods, the corneas were isolated for estimation of total protein content, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), total antioxidative capacity (TAC), total oxidative capacity (TOC) and oxidative stress index (OSI).
  
  
  RESULTS
  The present results of corneal total protein showed increment in the percentage change in normal healed groups after 1 day, 1 week and 4 weeks by values of 93%, 68% and 39%. In Q-switched Nd: YAG laser treated group the results showed better improvement in corneal protein than normal healed group with percentage changes of 58%, 29%, and 7.5% respectively. In SDS- PAGE, a protein band at 110 KD appeared in the migrating epithelium for both normal healed group and Q-switched Nd:YAG laser treated group with changes in the peaks intensities at middle and low molecular weight regions. Moreover, after 4 weeks the peak at 110 KD disappeared in the wounded epithelium treated with Q-switched Nd:YAG. After four weeks, the OSI in laser treated corneas showed pronounced balance between antioxidative capacityand oxidative capacity.
  
  
  CONCLUSIONS
  Anterior stromal puncture by Q-switched Nd:YAG laser is an effective, simple, safe and promising procedure to treat recurrent corneal erosion than normal healing.
authors: Ophthalmic Department, National Institute of Laser Enhanced Science, Cairo University, Egypt.
authors: Mohamed
authors: M
authors: Hamdy Abdelaziz
authors: Ophthalmic Department, National Institute of Laser Enhanced Science, Cairo University, Egypt.
authors: Dina
authors: D
authors: Fouad Ghoneim
authors: Department of Vision Science, Biophysics and Laser Science Unit, Research Institute of Ophthalmology, Giza, Egypt.
authors: Salwa
authors: S
authors: Abdelkawi Ahmed
authors: Ophthalmic Department, National Institute of Laser Enhanced Science, Cairo University, Egypt.
authors: Ibraheim Mohyeldin
authors: IM
authors: Taher
authors: Ophthalmic Department, National Institute of Laser Enhanced Science, Cairo University, Egypt.
authors: Ahmed Medhat
authors: AM
authors: Abdel-Salam
keywords: Q-Switched Nd; YAG lasers; corneal protein; wound healing
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