title: Limited effect of recombinant human mannose-binding lectin on the infection of novel influenza A (H7N9) virus in vitro.
abstract: Mannose-binding lectin (MBL), a pattern-recognition molecule in serum, recognizes specific hexose sugars rich in mannose and N-acetylglucosamine on bacterium, yeasts, viruses as well as apoptotic cells. It has been well-identified that MBL has antiviral effects via binding to seasonal influenza H1 and H3 subtype viruses. Influenza A (H7N9) virus, a novel reassortant virus to human population, possesses the surface hemagglutinin (HA) and neuraminidase (NA) genes from duck and wild-bird influenza viruses and internal genes from poultry H9N2 viruses. As of Dec 7th, 2014, a total of 467 human infections and 183 fatal cases have been identified. Here, recombinant human (rh) MBL was tested for its binding and effects on hemagglutination inhibition (HI) and NA activity inhibition (NAI) of avian H7N9, H9N2 and human H3N2 viruses. We discovered that rhMBL exhibited a strong binding to H7N9 virus as human H3N2 did at high virus titers. However, it performed a significantly weaker HI activity effect on H7N9 comparing to those of H3N2 and H9N2, even at a much higher concentration (3.67 ± 0.33 vs. 0.026 ± 0.001 and 0.083 ± 0.02 μg/mL, respectively). Similarly, minor NAI effect of rhMBL, even at up to 10 μg/mL, was found on H7N9 virus while it displayed significant effects on both H3N2 and H9N2 at a lowest concentration of 0.0807 ± 0.009 and 0.0625 μg/mL, respectively. The HI and NAI effects of rhMBL were calcium-dependent and mediated by lectin domain. Our findings suggest that MBL, the host innate molecule, has differential interference effects with human and avian influenza virus and limited antiviral effect against H7N9 virus.
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Jinlei
authors: J
authors: Guo
authors: Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resource and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, No.29 Wangjiang Road, Chengdu 610064, PR China.
authors: Yang
authors: Y
authors: Cao
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Kun
authors: K
authors: Qin
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Xiaopeng
authors: X
authors: Zhao
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Donghong
authors: D
authors: Wang
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Zi
authors: Z
authors: Li
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Li
authors: L
authors: Xin
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China.
authors: Yuelong
authors: Y
authors: Shu
authors: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, PR China. Electronic address: jfz@cnic.org.cn.
authors: Jianfang
authors: J
authors: Zhou
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004912:Erythrocytes / Q000821:virology; D006031:Glycosylation; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects / Q000378:metabolism / Q000472:pathogenicity; D064766:Influenza A Virus, H7N9 Subtype / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D005585:Influenza in Birds / Q000188:drug therapy / Q000821:virology; D037601:Mannose-Binding Lectin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014422:Turkeys
chemical_list: D000998:Antiviral Agents; D037601:Mannose-Binding Lectin; D011994:Recombinant Proteins; D009439:Neuraminidase
keywords: H7N9; Influenza A virus; Innate immunity; Mannose-binding lectin
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