title: Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles.
abstract: An in vitro imaging system to evaluate the stealth function of nanoparticles against mouse macrophages was established using fluorescent organosilica nanoparticles. Surface-functionalized organosilica nanoparticles with polyethylene glycol (PEG) were prepared by a one-step process, resulting in a brush-type PEG layer. A simultaneous dual-particle administration approach enabled us to evaluate the stealth function of nanoparticles with respect to single cells using time-lapse fluorescent microscopic imaging and flow cytometry analyses. Single-cell imaging and analysis revealed various patterns and kinetics of bare and PEGylated nanoparticle uptake. The PEGylated nanoparticles revealed a stealth function against most macrophages (PEG-sensitive macrophages); however, a stealth function against certain macrophages (PEG-insensitive macrophages) was not observed. We identified and characterized the PEG-resistant macrophages that could take up PEGylated nanoparticles at the same level as bare nanoparticles.
authors: Department of Anatomy and Cell Biology, The University of Tokushima Graduate School of Medical Sciences , 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
authors: Michihiro
authors: M
authors: Nakamura
authors: Koichiro
authors: K
authors: Hayashi
authors: Mutsuki
authors: M
authors: Nakano
authors: Takafumi
authors: T
authors: Kanadani
authors: Kazue
authors: K
authors: Miyamoto
authors: Toshinari
authors: T
authors: Kori
authors: Kazuki
authors: K
authors: Horikawa
mesh_terms: D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism*; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D015415:Biomarkers; D004337:Drug Carriers; D005456:Fluorescent Dyes; D017646:Organosilicon Compounds; D012821:Silanes; D011092:Polyethylene Glycols; C102833:(3-mercaptopropyl)trimethoxysilane
keywords: accelerated blood clearance; macrophages; polyethylene glycol; single-cell analysis; stealth nanoparticles