title: [Preparation and property of two specific anti-Helicobacter pylori immunoglobulins in egg yolk].
abstract: OBJECTIVE
  To prepare and purify anti-Helicobacter (H.) pylori Sydney Strain 1 (SS1) immunoglobulin Y (IgY) and anti-recombinant Ure I-UreB protein (rIB) IgY and investigate their antibacterial effects in vitro.
  
  
  METHODS
  rIB protein and SS1 cell solution were treated by ultrasonic disruption and used as immunogens to immune Leghorn hens. SS1-IgY and IB-IgY were derived from egg yolk with method of the water dilution (WD) combined with ammonium sulfate precipitations and analyzed by growth inhibition test (GIT) and rapid urease test (RUT).
  
  
  RESULTS
  Two specific IgY with purity of over 80% and titer over 1:12 800 were prepared successfully. Both SS1-IgY and IB-IgY inhibited the growth of H.pylori and reduced H.pylori urease activity in vitro markedly.
  
  
  CONCLUSIONS
  SS1-IgY and IB-IgY can inhibit the growth of H.pylori and reduce the urease activity in vitro.
authors: Sichuan Provincial Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014; Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
authors: Tingmin
authors: T
authors: Yang
authors: Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041; Department of Microbiology, Hubei University of Medicine, Shiyan 442000, China.
authors: Jing
authors: J
authors: Yang
authors: Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors: Baoning
authors: B
authors: Wang
authors: Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041; Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
authors: Xing
authors: X
authors: Pan
authors: Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
authors: Jianchun
authors: J
authors: Li
authors: Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
authors: Jie
authors: J
authors: Zhu
authors: Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
authors: Yongjun
authors: Y
authors: Zhou
authors: Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors: Mingyuan
authors: M
authors: Li
authors: Sichuan Provincial Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors: Suizhong
authors: S
authors: Cao
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D002645:Chickens; D004530:Egg Yolk; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology*; D014510:Urease / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C016512:IgY; D007136:Immunoglobulins; D014510:Urease