title: Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
abstract: We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Oliver
authors: O
authors: Otto
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Philipp
authors: P
authors: Rosendahl
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Alexander
authors: A
authors: Mietke
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Stefan
authors: S
authors: Golfier
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Christoph
authors: C
authors: Herold
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Daniel
authors: D
authors: Klaue
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Salvatore
authors: S
authors: Girardo
authors: Cavendish Laboratory, University of Cambridge, Cambridge, UK.
authors: Stefano
authors: 0000000197961956
authors: S
authors: Pagliara
authors: 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.
authors: Andrew
authors: A
authors: Ekpenyong
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Angela
authors: A
authors: Jacobi
authors: Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors: Manja
authors: M
authors: Wobus
authors: Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors: Nicole
authors: N
authors: Töpfner
authors: Cavendish Laboratory, University of Cambridge, Cambridge, UK.
authors: Ulrich F
authors: UF
authors: Keyser
authors: Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
authors: Jörg
authors: J
authors: Mansfeld
authors: 1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors: Elisabeth
authors: E
authors: Fischer-Friedrich
authors: 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.
authors: Jochen
authors: J
authors: Guck
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D019070:Cell Lineage; D048430:Cell Shape; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D046210:Microfluidic Analytical Techniques
chemical_list: D018952:Antigens, CD34; D015638:Cytochalasin D
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