title: In vitro inhibitory effects of terpenoids from Chloranthus multistachys on epithelial-mesenchymal transition via down-regulation of Runx2 activation in human breast cancer.
abstract: From Chloranthus multistachys, three terpenoids - lupeol (1), henrilabdane B (2), and istanbulin A (3) were isolated. Structures of compounds were established by NMR and MS. We reported here that ISTA (3) suppressed cell invasion, but lupeol (1) and henrilabdane B (2) did not. Furthermore, ISTA significantly inhibited the ability of adhesion and migration in vitro. Next, mechanisms of ISTA-induced inhibitory effects on in vitro metastasis were investigated. Sequential treatment data revealed that ISTA dramatically inhibited EGF-induced EMT. Western blot indicated that ISTA also significantly suppressed expression of E-cadherin, vimentin, and slug. In addition, ISTA inhibited Runx2 activation and phosph-Runx2 expression. Collectively, ISTA exhibited significant inhibitory effects on in vitro metastatic potential via inducing EMT inhibition, which may be associated with inhibition of transcriptional activity of Runx2.
authors: Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Jianjiang
authors: J
authors: Fu
authors: Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Shan
authors: S
authors: Wang
authors: Network and Educational Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Hong
authors: H
authors: Lu
authors: Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Junchao
authors: J
authors: Ma
authors: Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Xiaoqin
authors: X
authors: Ke
authors: Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors: Ting
authors: T
authors: Liu
authors: Department of Chemistry of Chinese materia medica, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, 18 Yunwan Rd, Nanchang 330004, China. Electronic address: loym999@163.com.
authors: Yongming
authors: Y
authors: Luo
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry; D012717:Sesquiterpenes / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology*; D000094042:Lupanes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D050659:Core Binding Factor Alpha 1 Subunit; D004224:Diterpenes; D053978:Pentacyclic Triterpenes; D012717:Sesquiterpenes; D013729:Terpenes; C493709:RUNX2 protein, human; C056597:istanbulin A; C010480:lupeol; D000094042:Lupanes
keywords: Chloranthus multistachys; EMT; In vitro; Istanbulin A; Runx2; Terpenoids