title: A paper based microfluidic device for easy detection of uric acid using positively charged gold nanoparticles.
abstract: A paper based microfluidic device is fabricated that can rapidly detect very low concentrations of uric acid (UA) using 3,5,3',5'-tetramethyl benzidine (TMB), H2O2 and positively charged gold nanoparticles ((+)AuNPs). In the presence of (+)AuNPs, H2O2 reacts with TMB to produce a bluish-green colour which becomes colourless on reaction with UA. This colorimetric method can detect as low as 8.1 ppm of UA within <20 minutes on white filter paper. This technique provides an alternative way for UA detection.
authors: Micro System Technology Laboratory, CSIR-Central Mechanical Engineering Research Institute (CMERI), Mahatma Gandhi Avenue, Durgapur-713209, India. n_chanda@cmeri.res.in.
authors: Anand
authors: A
authors: Kumar
authors: Abhiram
authors: A
authors: Hens
authors: Ravi Kumar
authors: RK
authors: Arun
authors: Monosree
authors: M
authors: Chatterjee
authors: Kuldeep
authors: K
authors: Mahato
authors: Keya
authors: K
authors: Layek
authors: Nripen
authors: N
authors: Chanda
mesh_terms: D001560:Benzidines / Q000737:chemistry; D002863:Chromogenic Compounds / Q000737:chemistry; D003124:Colorimetry / Q000295:instrumentation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010209:Paper; D014527:Uric Acid / Q000032:analysis*
chemical_list: D001560:Benzidines; D002863:Chromogenic Compounds; D014527:Uric Acid; C021758:3,3',5,5'-tetramethylbenzidine; D006046:Gold; D006861:Hydrogen Peroxide