title: Identification and characterization of a serious multidrug resistant Stenotrophomonas maltophilia strain in China.
abstract: An S. maltophilia strain named WJ66 was isolated from a patient; WJ66 showed resistance to more antibiotics than the other S. maltophilia strains. This bacteraemia is resistant to sulphonamides, or fluoroquinolones, while the representative strain of S. maltophilia, K279a, is sensitive to both. To explore drug resistance determinants of this strain, the draft genome sequence of WJ66 was determined and compared to other S. maltophilia sequences. Genome sequencing and genome-wide evolutionary analysis revealed that WJ66 was highly homologous with the strain K279a, but strain WJ66 contained additional antibiotic resistance genes. Further analysis confirmed that strain WJ66 contained an amino acid substitution (Q83L) in fluoroquinolone target GyrA and carried a class 1 integron, with an aadA2 gene in the resistance gene cassette. Homology analysis from the pathogen-host interaction database showed that strain WJ66 lacks raxST and raxA, which is consistent with K279a. Comparative genomic analyses revealed that subtle nucleotide differences contribute to various significant phenotypes in close genetic relationship strains.
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China ; The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China ; Department of Respiratory Diseases, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Yan
authors: Y
authors: Zhao
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
authors: Wenkai
authors: W
authors: Niu
authors: Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors: Yanxia
authors: Y
authors: Sun
authors: The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
authors: Huaijie
authors: H
authors: Hao
authors: Beijing Institute of Biotechnology, Beijing 100071, China.
authors: Dong
authors: D
authors: Yu
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
authors: Guangyang
authors: G
authors: Xu
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
authors: Xueyi
authors: X
authors: Shang
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
authors: Xueping
authors: X
authors: Tang
authors: Department of Respiratory Diseases, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Sijing
authors: S
authors: Lu
authors: Beijing Institute of Biotechnology, Beijing 100071, China.
authors: Junjie
authors: 0000-0002-2549-9261
authors: J
authors: Yue
authors: Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
authors: Yan
authors: Y
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D002681:China; D030541:Databases, Genetic; D004352:Drug Resistance, Microbial / Q000187:drug effects / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
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