title: Stretching of red blood cells using an electro-optics trap.
abstract: The stretching stiffness of Red Blood Cells (RBCs) was investigated using a combination of an AC dielectrophoretic apparatus and a single-beam optical tweezer. The experiments were performed at 10 MHz, a frequency high enough to avoid conductivity losses, but below the second turnover point between positive and negative dielectrophoresis. By measuring the geometrical parameters of single healthy human RBCs as a function of the applied voltage, the elastic modulus of RBCs was determined (µ = 1.80 ± 0.5 µN/m) and compared with similar values of the literature got by other techniques. The method is expected to be an easy-to-use, alternative tool to determine the mechano-elastic properties of living cells, and, on this basis, to distinguish healthy and diseased cells.
authors: Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, 6726 Szeged, Temesvari Krt. 62, Hungary ; Department of Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy, PoBox 35-43, Bucharest, Romania.
authors: Md Mozzammel
authors: MM
authors: Haque
authors: Department of Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy, PoBox 35-43, Bucharest, Romania.
authors: Mihaela G
authors: MG
authors: Moisescu
authors: Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, 6726 Szeged, Temesvari Krt. 62, Hungary.
authors: Sándor
authors: S
authors: Valkai
authors: Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, 6726 Szeged, Temesvari Krt. 62, Hungary.
authors: András
authors: A
authors: Dér
authors: Department of Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy, PoBox 35-43, Bucharest, Romania.
authors: Tudor
authors: T
authors: Savopol
keywords: (170.3880) Medical and biological imaging; (230.2090) Electro-optical devices; (350.4855) Optical tweezers or optical manipulation
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