title: In Vivo Anticoagulant and Thrombolytic Activities of a Fibrinolytic Serine Protease (Brevithrombolase) With the k-Carrageenan-Induced Rat Tail Thrombosis Model.
abstract: In the present study, in vivo thrombolysis efficiency of Brevithrombolase, a nontoxic fibrinolytic enzyme purified from Brevibacillus brevis strain FF02B, was affirmed by significant inhibition of thrombus formation in the k-carrageenan-induced rat tail, in a dose-dependent manner. Brevithrombolase at a dose of 600 µg/kg showed an efficacy that was comparable to streptokinase and plasmin, in dissolving in vivo thrombus of k-carrageenan-treated rats under identical conditions. The in vivo anticoagulant property of Brevithrombolase was demonstrated by its prolongation of activated partial thromboplastin time, prothrombin time, and thrombin time in Wistar rats. However, the Brevithrombolase-treated rats demonstrated an insignificant decrease in fibrinogen (Fg) level of plasma compared with Fg level of control group of rats corroborating in vivo as well as in vitro anticoagulant activity of Brevithrombolase is due to its hydrolytic action on thrombin. These findings unequivocally suggest that Brevithrombolase may serve a promising alternative to the commercial thrombolytic drugs.
authors: Department of Molecular Biology and Biotechnology, Microbial Biotechnology and Protein Research Laboratory, School of Science, Tezpur University, Tezpur, Assam, India.
authors: Sourav
authors: S
authors: Majumdar
authors: Division of Pharmaceutical Technology, Defense Research Laboratory, Tezpur, Assam, India.
authors: Pronobesh
authors: P
authors: Chattopadhyay
authors: Department of Molecular Biology and Biotechnology, Microbial Biotechnology and Protein Research Laboratory, School of Science, Tezpur University, Tezpur, Assam, India akm@tezu.ernet.in.
authors: Ashis K
authors: AK
authors: Mukherjee
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D057057:Serine Proteases / Q000494:pharmacology / Q000627:therapeutic use*; D013917:Thrombin / Q000378:metabolism; D013927:Thrombosis / Q000188:drug therapy*
chemical_list: D000925:Anticoagulants; D005343:Fibrinolytic Agents; D057057:Serine Proteases; D013917:Thrombin
keywords: anticoagulant; nontoxic; prothrombin time; thrombin time; thrombolytic drug; thrombosis