title: Curcumin Suppresses the Production of Pro-inflammatory Cytokine Interleukin-18 in Lipopolysaccharide Stimulated Murine Macrophage-Like Cells.
abstract: Curcumin is a major bioactive compound of turmeric that exerts its anti-inflammatory effects by suppressing the many pro-inflammatory cytokines and chemokines in a number of cell types and pathologic conditions. Interleukin-18 (IL-18) is a novel pro-inflammatory cytokine which plays an important role not only in generating Th1 responses but also in inducing severe inflammatory reactions. As curcumin induced inhibition of IL-18 production in keratinocytes and mice is well known, effect of curcumin on IL-18 release in macrophages remains unknown. Hence, this present study has been designed to evaluate the effect of curcumin on IL-18 production and necrotic cell death in murine macrophages-like cells treated with or without lipopolysaccharide (LPS). The IL-18 secretion in cell culture supernatants was assayed by enzyme-linked immunosorbent assay and cytotoxicity was determined by lactate dehydrogenase release assay. Our results demonstrate that curcumin significantly inhibited the production of pro-inflammatory cytokine IL-18 in E.coli LPS stimulated murine macrophage-like cells RAW264.7 in a concentration-dependent manner. Interestingly, curcumin had no cytotoxic effect on murine macrophage-like cells. Our findings suggest that curcumin may be used as a potential therapeutic agent for the treatment of inflammatory diseases.
authors: Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 024 India.
authors: Renu
authors: R
authors: Yadav
authors: Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 024 India ; Department of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra, 282 001 India.
authors: Babban
authors: B
authors: Jee
authors: Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 024 India.
authors: Sudhir Kumar
authors: SK
authors: Awasthi
keywords: Curcumin; IL-18; Murine macrophage-like cells; Necrosis
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