title: The prognostic value of circulating microRNAs in heart failure: preliminary results from a genome-wide expression study.
abstract: BACKGROUND
  Recent studies have demonstrated the potential of microRNAs (miRNA) as biomarkers in various cardiovascular disorders. The aim of the present study was to quantitatively evaluate the expression levels of miRNAs in patients with chronic congestive heart failure (CHF) in order to identify differential expression profiles as biomarkers with prognostic values.
  
  
  METHODS
  The study included 20 clinically stable [New York Heart Association (NYHA) II] and 22 decompensated (NYHA III and IV) CHF patients and 15 healthy controls. miRNA profiling was performed using a microarray method. Dysregulated miRNAs were evaluated for their biomarker potential.
  
  
  RESULTS
  Microarray profiling revealed an increase in the expression of miR-21, miR-650, miR-744, miR-516-5p, miR-1292, miR-182, miR-1228, miR-595, miR-663b, miR-1296, miR-1825, miR-299-3p, miR-662 miR-122, miR-3148 and miR-518e and a decrease in the expression of miR-129-3p, miR-3155, miR-3175, miR-583, miR-568, miR-30d, miR-200a-star, miR-1979, miR-371-3p, miR-155-star and miR-502-5p in sera of CHF patients. The prognostic value of miR-182 [area under the curve (AUC) 0.695] was found to be superior to pro-brain type natriuretic peptide (NT-proBNP; AUC 0.350) and high-sensitivity C-reactive protein (hs-CRP) (AUC 0.475) by receiver operator characteristic (ROC) analysis. Cox regression analysis showed that miR-182 could predict cardiovascular mortality (P = 0.032).
  
  
  CONCLUSIONS
  We demonstrated the increased expression levels of circulating miRNAs in CHF as compared with controls. Moreover, miR-182 was found to be a potential prognostic marker in CHF.
authors: aRize Kackar Government Hospital, Department of Cardiology, Rize bIstanbul University, Faculty of Medicine, Department of Internal Medicine, Division of Medical Genetics, Istanbul cIstanbul University, Cerrahpasa Faculty of Medicine, Department of Cardiology, Istanbul dIstanbul University, Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkey.
authors: Huseyin Altug
authors: HA
authors: Cakmak
authors: Ender
authors: E
authors: Coskunpinar
authors: Baris
authors: B
authors: Ikitimur
authors: Hasan Ali
authors: HA
authors: Barman
authors: Bilgehan
authors: B
authors: Karadag
authors: Necip Ozan
authors: NO
authors: Tiryakioglu
authors: Kadriye
authors: K
authors: Kahraman
authors: Vural Ali
authors: VA
authors: Vural
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study / Q000379:methods; D006333:Heart Failure / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D002097:C-Reactive Protein