title: Differentiations of chitin content and surface morphologies of chitins extracted from male and female grasshopper species.
abstract: In this study, we used Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), and scanning electron microscopy (SEM) to investigate chitin structure isolated from both sexes of four grasshopper species. FT-IR, EA, XRD, and TGA showed that the chitin was in the alpha form. With respect to gender, two main differences were observed. First, we observed that the quantity of chitin was greater in males than in females and the dry weight of chitin between species ranged from 4.71% to 11.84%. Second, using SEM, we observed that the male chitin surface structure contained 25-90 nm wide nanofibers and 90-250 nm nanopores, while no pores or nanofibers were observed in the chitin surface structure of the majority of females (nanofibers were observed only in M. desertus females). In contrast, the elemental analysis, thermal properties, and crystalline index values for chitin were similar in males and females. Also, we carried out enzymatic digestion of the isolated chitins using commercial chitinase from Streptomyces griseus. We observed that there were no big differences in digestion rate of the chitins from both sexes and commercial chitin. The digestion rates were for grasshoppers' chitins; 88.45-95.48% and for commercial chitin; 94.95%.
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100, Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey.
authors: Murat
authors: M
authors: Kaya
authors: Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey; Department of Biology, Vytautas Magnus University, 44404 Kaunas, Lithuania.
authors: Evaldas
authors: E
authors: Lelešius
authors: Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey; Department of Biology, Vytautas Magnus University, 44404 Kaunas, Lithuania.
authors: Radvilė
authors: R
authors: Nagrockaitė
authors: Selcuk University, Faculty of Science, Department of Chemistry, 42075, Konya, Turkey.
authors: Idris
authors: I
authors: Sargin
authors: Selcuk University, Faculty of Science, Department of Biochemistry, 42075, Konya, Turkey.
authors: Gulsin
authors: G
authors: Arslan
authors: Guzelyurt Vocational School, Aksaray University, Guzelyurt, Aksaray, Turkey.
authors: Abbas
authors: A
authors: Mol
authors: Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey; Department of Chemistry, Faculty of Science and Letters, Aksaray University, Aksaray, Turkey.
authors: Talat
authors: T
authors: Baran
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100, Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey.
authors: Esra
authors: E
authors: Can
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100, Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100, Aksaray, Turkey.
authors: Betul
authors: B
authors: Bitim
mesh_terms: D000818:Animals; D002686:Chitin / Q000737:chemistry* / Q000302:isolation & purification; D002688:Chitinases; D005260:Female; D006110:Grasshoppers / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D012727:Sex Characteristics; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002686:Chitin; D002688:Chitinases
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