title: High-density SNP arrays improve detection of HER2 amplification and polyploidy in breast tumors.
abstract: BACKGROUND
  Human epidermal growth factor receptor-2 (HER2) overexpression and gene amplification are currently established by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), respectively. This study investigates whether high-density single nucleotide polymorphism (SNP) arrays can provide additional diagnostic power to assess HER2 gene status.
  
  
  METHODS
  DNA from 65 breast tumor samples previously diagnosed by HER2 IHC and FISH analysis were blinded and examined for HER2 copy number variation employing SNP array analysis.
  
  
  RESULTS
  SNP array analysis identified 24 (37%) samples with selective amplification or imbalance of the HER2 region in the q-arm of chromosome 17. In contrast, only 15 (23%) tumors were found to have HER2 amplification by IHC and FISH analysis. In total, there was a discrepancy in 19 (29%) samples between SNP array and IHC/FISH analysis. In 12 of these cases, the discrepancy towards FISH could be attributed to concomitant amplification or deletion of the centromeric region, which harbors the FISH reference probe sequence. In 3 tumors, repeated IHC/FISH analysis revealed that the original IHC/FISH analysis had failed to indicate the correct HER2 expression level. Finally, the SNP array analysis revealed that more than two thirds of the samples exhibited polyploidy that was unrecognized by conventional FISH.
  
  
  CONCLUSIONS
  Collectively, the data show that determination of HER2 copy number variations by SNP array-based genomic segmentation analysis is an effective supplement to IHC/FISH HER2 analysis that, by providing additional diagnostic sensitivity and accuracy, may elect more women for targeted treatment with HER2 inhibitors.
authors: Center for Genomic Medicine, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. tvoh@rh.dk.
authors: Thomas V O
authors: TV
authors: Hansen
authors: Center for Genomic Medicine, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. jonas.vikesaa@regionh.dk.
authors: Jonas
authors: J
authors: Vikesaa
authors: Center for Genomic Medicine, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. sine_buhl@hotmail.com.
authors: Sine S
authors: SS
authors: Buhl
authors: Department of Pathology, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. Henrik.Holm.Rossing@regionh.dk.
authors: Henrik H
authors: HH
authors: Rossing
authors: Department of Pathology, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. Vera.Timmermans.Wielenga@regionh.dk.
authors: Vera
authors: V
authors: Timmermans-Wielenga
authors: Center for Genomic Medicine, Copenhagen University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. fcn@rh.dk.
authors: Finn C
authors: FC
authors: Nielsen
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D056915:DNA Copy Number Variations; D005260:Female; D005784:Gene Amplification; D023281:Genomics / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases
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