title: Effect of the combined extracts of herba epimedii and fructus ligustri lucidi on sex hormone functional levels in osteoporosis rats.
abstract: The combination of Herba Epimedii and Fructus Ligustri Lucidi has been used to treat osteoporosis for almost 50 years by Professor Shizeng Li, a famous doctor of traditional Chinese medicine (TCM). However, it is unclear whether the combination of the effective constituents of the two herbs may have a protective influence on the skeleton. In the present study, we investigated the effects of the combination extracts of Herba Epimedii and Fructus Ligustri Lucidi on rat model of osteoporosis induced by retinoic acid by gavage. With administrations of the combination extracts of the two herbs (50, 100, and 200 mg/kg/day) via oral gavage for 3 weeks, bone mineral density (BMD), femur histomorphometry, some sex hormones, and sex hormone receptors were measured. Results showed that the combined extracts could increase BMD, affect bone histomorphometry, coordinate the sex hormones at the level of hypothalamus-pituitary-gonad axis, and increase the protein and mRNA expressions of sex hormone receptors. The findings suggested that the combination extracts of Herba Epimedii and Fructus Ligustri Lucidi might be beneficial as an alternative medicine for the prevention and treatment of osteoporosis.
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: RenHui
authors: 0000-0001-9660-1924
authors: R
authors: Liu
authors: Pharmacy of Traditional Chinese Medicine, Beijing Jishuitan Hospital, No. 10 Xinjiekou East Street, Xicheng District, Beijing 100035, China.
authors: Xue
authors: X
authors: Kang
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: LiPing
authors: 0000-0003-0010-139X
authors: L
authors: Xu
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: HongLei
authors: H
authors: Nian
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: XinWei
authors: X
authors: Yang
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: HaoTian
authors: H
authors: Shi
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No. 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China.
authors: XiuJuan
authors: X
authors: Wang
references: Cell. 2006 Apr 21;125(2):247-60
references: 16630814
references: Clin Endocrinol (Oxf). 2007 Aug;67(2):295-303
references: 17555504
references: Bone. 2013 Mar;53(1):134-44
references: 23201268
references: Calcif Tissue Int. 2014 Apr;94(4):433-41
references: 24343527
references: Zhongguo Gu Shang. 2009 Apr;22(4):271-3
references: 19408755
references: Biochimie. 2012 Dec;94(12 ):2514-22
references: 22796380
references: J Biol Chem. 2007 Aug 31;282(35):25501-8
references: 17609204
references: J Clin Endocrinol Metab. 2006 Apr;91(4):1261-7
references: 16403818
references: J Bone Miner Res. 1998 May;13(5):763-73
references: 9610739
references: JAMA. 2002 Aug 28;288(8):980-7
references: 12190368
references: Zhongguo Zhong Yao Za Zhi. 2013 Dec;38(23 ):4124-8
references: 24791502
references: Menopause. 2010 Nov-Dec;17 (6):1174-81
references: 20711082
references: N Engl J Med. 2003 Jan 23;348(4):287-94
references: 12540641
references: J Bone Miner Res. 1987 Dec;2(6):595-610
references: 3455637
references: Osteoporos Int. 2012 Mar;23(3):1131-40
references: 21927920
references: Arq Bras Endocrinol Metabol. 2014 Mar;58(2):172-81
references: 24830594
references: Phytomedicine. 2011 Jul 15;18(10):868-72
references: 21377852
references: Asian J Androl. 2014 Mar-Apr;16(2):213-22
references: 24385015
references: Br J Nutr. 2012 Jul 14;108(1):92-101
references: 22018100
references: Bone. 2006 Feb;38(2 Suppl 1):S4-9
references: 16455317
references: Osteoporos Int. 2007 Jan;18(1):85-92
references: 17019521
references: Menopause. 2005 Nov-Dec;12(6):755-62
references: 16278620
references: Am J Clin Nutr. 2003 Sep;78(3 Suppl):593S-609S
references: 12936954
references: Clin Cornerstone. 2003;Suppl 2:S13-21
references: 15035555
references: Technol Health Care. 1998 Dec;6(5-6):321-7
references: 10100935
references: J Clin Endocrinol Metab. 2003 Nov;88(11):5240-7
references: 14602756
references: JAMA. 2002 Aug 21;288(7):872-81
references: 12186605
references: Bone. 2002 Dec;31(6):685-9
references: 12531562
references: Curr Osteoporos Rep. 2010 Jun;8(2):60-7
references: 20425612
references: J Tradit Complement Med. 2013 Apr;3(2):82-7
references: 24716161
references: Biol Pharm Bull. 2010;33(3):455-60
references: 20190409
references: Minerva Med. 2000 Nov-Dec;91(11-12):283-9
references: 11253709
references: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9416-21
references: 12872002
references: Pharmazie. 2013 Sep;68(9):713-22
references: 24147339
references: Zhongguo Zhong Yao Za Zhi. 2008 Apr;33(8):928-31
references: 18619355
references: Best Pract Res Clin Rheumatol. 2013 Dec;27(6):743-55
references: 24836333