title: High-sensitivity N-glycoproteomic analysis of mouse brain tissue by protein extraction with a mild detergent of N-dodecyl β-D-maltoside.
abstract: N-dodecyl β-D-maltoside (DDM), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for N-glycoproteomic analysis of minute protein sample from mouse brain tissue. After combining with the capillary-based glycoproteomic reactor, 281 N-glycosylation sites were successfully characterized from 50 μg of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies.
authors: Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS) , Dalian 116023, China.
authors: Jing
authors: J
authors: Liu
authors: Fangjun
authors: F
authors: Wang
authors: Jiawei
authors: J
authors: Mao
authors: Zhang
authors: Z
authors: Zhang
authors: Zheyi
authors: Z
authors: Liu
authors: Guang
authors: G
authors: Huang
authors: Kai
authors: K
authors: Cheng
authors: Hanfa
authors: H
authors: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003902:Detergents / Q000737:chemistry*; D004867:Equipment Design; D005960:Glucosides / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D003902:Detergents; D005960:Glucosides; D006023:Glycoproteins; C040358:dodecyl maltoside