title: A sensitive chemiluminescent immunoassay for point-of-care testing of repaglinide in natural dietary supplements and serum.
abstract: For point-of-care testing of the illegal fortification of repaglinide (Rep) in natural dietary supplements, a competitive chemiluminescent immunoassay (CLIA) was established, using a horseradish peroxidase (HRP)-luminol-H2O2 system for signal amplification. Polyclonal antibodies for Rep were produced via immunization technique. Following optimization of the enzyme reaction time and concentrations of antibody and coating antigen, the method showed a limit of quantification (LOQ) of 1.0 ng/mL in PBS and limit of detection (LOD) of 8.3 ng/mL in serum and 6.0 ng/mL in blank tablets. When applied in natural dietary supplements, the method provided results consistent with those from HPLC, suggesting that the proposed method could be used for rapid screening of Rep in natural dietary supplements and detecting Rep in serum after administration.
authors: College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, China.
authors: Lei
authors: L
authors: Zheng
authors: Jing
authors: J
authors: Wang
authors: Yufen
authors: Y
authors: Wang
authors: Zhaorui
authors: Z
authors: Song
authors: Yaqing
authors: Y
authors: Dong
authors: Yongmei
authors: Y
authors: Yin
authors: Sergei A
authors: SA
authors: Eremin
authors: Meng
authors: M
authors: Meng
authors: Rimo
authors: R
authors: Xi
mesh_terms: D002219:Carbamates / Q000032:analysis* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D049449:Luminescence; D010880:Piperidines / Q000032:analysis* / Q000097:blood*; D019095:Point-of-Care Systems
chemical_list: D002219:Carbamates; D010880:Piperidines; C072379:repaglinide