title: Effects of three kinds of curcuminoids on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose levels.
abstract: OBJECTIVE
  Curcuminoids are the main bioactive constituents of the rhizome of turmeric. Erythrocytes lesions in diabetes are probably related to hyperglycemia and protein glycation. It has been reported that curcumin prevent lipid peroxidation. However, reports on the effects of demethoxycurcumin and bis-demethoxycurcumin on human erythrocytes at high glucose levels are scarce. Our aim is to investigate the effect of curcuminoids on oxidative stress and membrane of erythrocytes exposed to hyperglycemic condition.
  
  
  METHODS
  In this study, the different blood samples were treated with two doses of glucose (10 or 30 mM) to mimic hyperglycemia in the presence or absence of three kinds of curcuminoids (5 or 10 μM) in a medium at 37 °C for 24 h (Each experiment consists of 20 blood samples from 10 male and 10 female volunteers). The malondialdehyde was checked by HPLC, antioxidase (GSH and GSSG) were measured by LC/MS, SOD was checked by WST-1 kit, morphology and phospholipid symmetry were detected by flow cytometry, confocal scanning microscope and scanning electron microscope.
  
  
  RESULTS
  The results illustrated that all three curcuminoids reduce oxidative stress damage on the membrane and maintain a better profile for erythrocytes. Furthermore, three curcuminoids had benefit effects on antioxidase.
  
  
  CONCLUSIONS
  The three kinds of curcuminoids supplementation may prevent lipid peroxidation at different intensity and membrane dysfunction of human erythrocytes in hyperglycemia.
authors: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Wei
authors: W
authors: Yang
authors: Juan
authors: J
authors: Fu
authors: Miao
authors: M
authors: Yu
authors: Di
authors: D
authors: Wang
authors: Ying
authors: Y
authors: Rong
authors: Ping
authors: P
authors: Yao
authors: Andreas K
authors: AK
authors: Nüssler
authors: Hong
authors: H
authors: Yan
authors: Liegang
authors: L
authors: Liu
mesh_terms: D002462:Cell Membrane / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D036381:Diarylheptanoids; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D036381:Diarylheptanoids; C034786:bisdemethoxycurcumin; D003474:Curcumin; D005947:Glucose; C050229:demethoxycurcumin