title: Chimeric virus-like particles made using GAG and M1 capsid proteins providing dual drug delivery and vaccination platform.
abstract: Nanobiomaterials such as enveloped virus-like particles with specificity can serve a dual function of vaccination and drug delivery system. Here, we expressed colon carcinoma cell-targeting chimeric virus-like particles (VLPs) made using two capsid proteins, gag and M1 from influenza virus A/swine flu/Iowa/15/30/H1N1 in silkworms. These chimeric VLPs displayed a glycosylphosphatidylinositol-anchored single-chain variable fragment region targeting colon carcinoma cells, and their shape was smooth, with an average particle size of 21 nm in diameter. Large unilamellar vesicles made from DOPC:DOPA (2:1) containing calcein-AM (10 μM) or doxorubicin (13.7 nM) were used to package chimeric VLPs. VLPs showed high specificity in targeting cancer cells and delivered the dye and drug to cells successfully. Chimeric VLPs were injected into BALB/c mice, and the serum showed specificity for M1 protein as a model.
authors: Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, ‡Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University , 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors: Vipin K
authors: VK
authors: Deo
authors: Tatsuya
authors: T
authors: Kato
authors: Enoch Y
authors: EY
authors: Park
mesh_terms: D000818:Animals; D001702:Biopharmaceutics; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D015683:Gene Products, gag / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008051:Lipid Bilayers / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000737:chemistry*; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D004337:Drug Carriers; D015683:Gene Products, gag; D008051:Lipid Bilayers; C488936:M1 protein, Influenza A virus; D057127:Single-Chain Antibodies; D058425:Vaccines, Virus-Like Particle; D014763:Viral Matrix Proteins
keywords: chimeric; drug delivery system; silkworm expression system; vaccination; virus-like particles