title: A spectrum of HRT-dependent hypersensitive responses elicited by the 52 amino acid N-terminus of turnip crinkle virus capsid protein and its mutants.
abstract: The capsid protein (CP) of turnip crinkle virus (TCV) is the elicitor of hypersensitive response (HR) and resistance mediated by the resistance protein HRT in the Di-17 ecotype of Arabidopsis. Here we identified the N-terminal 52-amino-acid R domain of TCV CP as the elicitor of HRT-dependent HR in Nicotiana benthamiana. Mutating this domain at position 6 (R6A), but not at positions 8 (R8A) or 14 (G14A), abolished HR in N. benthamiana. However, on Di-17 Arabidopsis leaves only R8A R domain elicited visible epidermal HR. When incorporated in infectious TCV RNAs, R8A and G14A mutations exerted dramatically different effects in Di-17 plants, as R8A caused systemic cell death whereas G14A led to complete restriction of the mutant virus. This continual spectrum of HR and resistance responses elicited by various R domain mutants suggests that the CP-HRT interaction could be perturbed by conformational changes in the R domain of TCV CP.
authors: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, United States.
authors: Sung-Hwan
authors: SH
authors: Kang
authors: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, United States; Department of Plant Pathology, The Ohio State University, Wooster, OH 44691, United States. Electronic address: qu.28@osu.edu.
authors: Feng
authors: F
authors: Qu
authors: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, United States. Electronic address: jmorris1@unl.edu.
authors: T Jack
authors: TJ
authors: Morris
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000821:virology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D019184:Carmovirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D009154:Mutation; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D014026:Nicotiana / Q000276:immunology / Q000821:virology*
chemical_list: D036022:Capsid Proteins
keywords: Capsid protein; Hypersensitive response; Plant virus; Resistance protein; Turnip crinkle virus