title: Tunable nanostructured coating for the capture and selective release of viable circulating tumor cells.
abstract: A layer-by-layer gelatin nanocoating is presented for use as a tunable, dual response biomaterial for the capture and release of circulating tumor cells (CTCs) from cancer patient blood. The entire nanocoating can be dissolved from the surface of microfluidic devices through biologically compatible temperature shifts. Alternatively, individual CTCs can be released through locally applied mechanical stress.
authors: Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Building 114, 16th Street, Charlestown, MA, 02129, USA; Shriners Hospital for Children, Harvard Medical School, 51 Blossom Street, Boston, MA, 02114, USA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School 55, 55 Fruit Street, Boston, MA, 02114, USA.
authors: Eduardo
authors: E
authors: Reátegui
authors: Nicola
authors: N
authors: Aceto
authors: Eugene J
authors: EJ
authors: Lim
authors: James P
authors: JP
authors: Sullivan
authors: Anne E
authors: AE
authors: Jensen
authors: Mahnaz
authors: M
authors: Zeinali
authors: Joseph M
authors: JM
authors: Martel
authors: Alexander J
authors: AJ
authors: Aranyosi
authors: Wei
authors: W
authors: Li
authors: Steven
authors: S
authors: Castleberry
authors: Aditya
authors: A
authors: Bardia
authors: Lecia V
authors: LV
authors: Sequist
authors: Daniel A
authors: DA
authors: Haber
authors: Shyamala
authors: S
authors: Maheswaran
authors: Paula T
authors: PT
authors: Hammond
authors: Mehmet
authors: M
authors: Toner
authors: Shannon L
authors: SL
authors: Stott
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D044963:Biopsy, Fine-Needle; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D004867:Equipment Design; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics; D008297:Male; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D049329:Nanostructures / Q000737:chemistry; D009360:Neoplastic Cells, Circulating / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000097:blood; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods; D013314:Stress, Mechanical; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin
keywords: circulating tumor cells; circulating tumor clusters; microfluidics; nanostructured coatings; single cell sequencing
references: Adv Mater. 2013 Apr 24;25(16):2368-73
references: 23495071
references: Anal Bioanal Chem. 2013 Sep;405(23):7377-82
references: 23828210
references: Nat Nanotechnol. 2013 Oct;8(10):735-41
references: 24077027
references: ACS Nano. 2014 Mar 25;8(3):1995-2017
references: 24601556
references: Cell. 2014 Aug 28;158(5):1110-22
references: 25171411
references: Cell Rep. 2014 May 8;7(3):645-53
references: 24746818
references: Anal Chem. 2014 May 6;86(9):4618-26
references: 24716801
references: Adv Mater. 2013 Jun 4;25(21):2897-902
references: 23529932
references: N Engl J Med. 2004 Aug 19;351(8):781-91
references: 15317891
references: Br J Cancer. 1988 Jan;57(1):32-5
references: 3279993
references: Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17073-4
references: 17090687
references: Nature. 2007 Dec 20;450(7173):1235-9
references: 18097410
references: J Am Chem Soc. 2008 Jul 9;130(27):8633-41
references: 18557614
references: N Engl J Med. 2008 Jul 24;359(4):366-77
references: 18596266
references: Nat Nanotechnol. 2007 Aug;2(8):469-78
references: 18654343
references: Nat Nanotechnol. 2008 Nov;3(11):671-5
references: 18989333
references: Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18392-7
references: 20930119
references: J Cell Biol. 2011 Feb 7;192(3):373-82
references: 21300848
references: Angew Chem Int Ed Engl. 2011 Mar 21;50(13):3084-8
references: 21374764
references: Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4852-7
references: 21368175
references: Langmuir. 2011 Apr 5;27(7):4257-64
references: 21401041
references: Adv Mater. 2011 Oct 11;23(38):4376-80
references: 21882263
references: Phys Biol. 2012 Feb;9(1):016001
references: 22306705
references: Anal Chem. 2012 Apr 17;84(8):3682-8
references: 22414137
references: PLoS One. 2012;7(5):e33788
references: 22586443
references: Nature. 2012 Jul 26;487(7408):510-3
references: 22763454
references: Am J Pathol. 2012 Nov;181(5):1573-84
references: 23022210
references: Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19626-31
references: 23150586
references: Science. 2013 Feb 1;339(6119):580-4
references: 23372014
references: Adv Mater. 2013 Mar 20;25(11):1547-51
references: 23255101
references: Angew Chem Int Ed Engl. 2013 Mar 18;52(12):3379-83
references: 23436302
references: Sci Transl Med. 2013 Apr 3;5(179):179ra47
references: 23552373