title: Effects of Flavonoid-rich Plant Extracts on In vitro Ruminal Methanogenesis, Microbial Populations and Fermentation Characteristics.
abstract: The objective of this study was to evaluate the in vitro effects of flavonoid-rich plant extracts (PE) on ruminal fermentation characteristics and methane emission by studying their effectiveness for methanogenesis in the rumen. A fistulated Holstein cow was used as a donor of rumen fluid. The PE (Punica granatum, Betula schmidtii, Ginkgo biloba, Camellia japonica, and Cudrania tricuspidata) known to have high concentrations of flavonoid were added to an in vitro fermentation incubated with rumen fluid. Total gas production and microbial growth with all PE was higher than that of the control at 24 h incubation, while the methane emission was significantly lower (p<0.05) than that of the control. The decrease in methane accumulation relative to the control was 47.6%, 39.6%, 46.7%, 47.9%, and 48.8% for Punica, Betula, Ginkgo, Camellia, and Cudrania treatments, respectively. Ciliate populations were reduced by more than 60% in flavonoid-rich PE treatments. The Fibrobacter succinogenes diversity in all added flavonoid-rich PE was shown to increase, while the Ruminoccocus albus and R. flavefaciens populations in all PE decreased as compared with the control. In particular, the F. succinogenes community with the addition of Birch extract increased to a greater extent than that of others. In conclusion, the results of this study showed that flavonoid-rich PE decreased ruminal methane emission without adversely affecting ruminal fermentation characteristics in vitro in 24 h incubation time, suggesting that the flavonoid-rich PE have potential possibility as bio-active regulator for ruminants.
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2R3, Canada .
authors: Eun T
authors: ET
authors: Kim
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2R3, Canada .
authors: Le Luo
authors: le L
authors: Guan
authors: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 660-701, Korea .
authors: Shin J
authors: SJ
authors: Lee
authors: National Institute of Animal Science, RDA, Pyeongchang 232-950, Korea .
authors: Sang M
authors: SM
authors: Lee
authors: Department of Animal Science and Technology, Sunchon National Uuniversity, Suncheon 540-742, Korea .
authors: Sang S
authors: SS
authors: Lee
authors: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 660-701, Korea .
authors: Il D
authors: ID
authors: Lee
authors: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 660-701, Korea .
authors: Su K
authors: SK
authors: Lee
authors: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 660-701, Korea .
authors: Sung S
authors: SS
authors: Lee
keywords: Flavonoid-rich Plant; Methane Emission; Microbial Growth; Rumen Anaerobic Microbes; Ruminal Fermentation
references: J Anim Sci. 1995 Aug;73(8):2483-92
references: 8567486
references: Lett Appl Microbiol. 2006 Mar;42(3):222-8
references: 16478508
references: Biomed Res Int. 2013;2013:349129
references: 24175289
references: J Dairy Sci. 2009 Nov;92(11):5512-22
references: 19841214
references: FEMS Microbiol Ecol. 2007 Dec;62(3):313-22
references: 17949432
references: Appl Environ Microbiol. 2011 Apr;77(8):2634-9
references: 21357427
references: Phytochemistry. 2010 Aug;71(11-12):1198-222
references: 20570294
references: Lett Appl Microbiol. 2001 Jan;32(1):47-51
references: 11169041
references: FEMS Microbiol Lett. 2001 Nov 13;204(2):361-6
references: 11731149
references: J Anim Sci. 2012 Dec;90(13):4975-84
references: 22829622
references: Int J Antimicrob Agents. 2005 Nov;26(5):343-56
references: 16323269
references: Biochem J. 1948;43(1):99-109
references: 16748377
references: Appl Environ Microbiol. 1977 Sep;34(3):297-301
references: 562131
references: Appl Environ Microbiol. 2006 Jan;72(1):200-6
references: 16391043
references: J Dairy Sci. 2007 Jul;90(7):3456-66
references: 17582129
references: J Dairy Sci. 2004 Jul;87(7):2239-47
references: 15328238
references: J Dairy Sci. 2007 Feb;90(2):886-97
references: 17235165
references: FEMS Microbiol Ecol. 2006 Dec;58(3):572-82
references: 17117998