title: Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.
abstract: In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.
authors: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.
authors: Guowei
authors: G
authors: Zhu
authors: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.
authors: Chongran
authors: C
authors: Sun
authors: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.
authors: Weiguo
authors: W
authors: Liu
keywords: differentiation; neural regeneration; neural stem cells; neuron; neurotrophin-3; oligodendrocytes; stem cells
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