title: An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration.
abstract: Tissue-derived extracellular matrix (ECM) biomaterials to regenerate the meniscus have gained increasing attention in treating meniscus injuries and diseases, particularly for aged persons and athletes. However, ECM scaffold has poor cell infiltration and can only be implanted using surgical procedures. To overcome these limitations, we developed an injectable ECM hydrogel material from porcine meniscus via modified decellularization and enzymatic digestion. This meniscus-derived ECM hydrogel exhibited a fibrous morphology with tunable compression and initial modulus. It had a good injectability evidenced by syringe injection into mouse subcutaneous tissue. The hydrogel showed good cellular compatibility by promoting the growth of both bovine chondrocytes and mouse 3T3 fibroblasts encapsulated in the hydrogel for 2 weeks. It also promoted cell infiltration as shown in both in vitro cell culture and in vivo mouse subcutaneous implantation. The in vivo study revealed that the ECM hydrogel possessed good tissue compatibility after 7 days of implantation. The results support the great potential of the newly produced injectable meniscus-derived ECM hydrogel specifically for meniscus repair and regeneration.
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Jinglei
authors: J
authors: Wu
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Qing
authors: Q
authors: Ding
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Ahana
authors: A
authors: Dutta
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Yezhou
authors: Y
authors: Wang
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Yi-Hui
authors: YH
authors: Huang
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors: Hong
authors: H
authors: Weng
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: Liping
authors: L
authors: Tang
authors: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA; Joint Graduate Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: yihong@uta.edu.
authors: Yi
authors: Y
authors: Hong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D007211:Indoles / Q000378:metabolism; D007267:Injections; D008592:Menisci, Tibial / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012038:Regeneration / Q000187:drug effects*; D034421:Sus scrofa; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D020099:Coated Materials, Biocompatible; D007211:Indoles; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C007293:DAPI
keywords: Decellularization; Extracellular matrix; Injectable hydrogel; Meniscus regeneration; Porcine meniscus