title: [Effect of acupuncture intervention on 14-3-3 expression in cerebral cortex of hypoxic-ischemic brain damage rats].
abstract: OBJECTIVE
  To observe the effect of acupuncture therapy on 14-3-3, Bcl-2 and Bax expression levels in the cerebral cortex in neonatal rats with hypoxic-ischemic brain damage(HIBD).
  
  
  METHODS
  Timed pregnant Sprague-Dawley rat dams were delivered either vaginally (normal group), or by C-section (sham-operation group) or by C-section with 5 min of global anoxia (anoxia group), with 8 rats in each group. The rat pups of the anoxia group were randomly divided into model group and acupuncture group (n =8). Acupuncture stimulation of "Naosanzhen" "Niesanzhen" and "Zhisanzhen" acupoints was given begin- ning from the 14th day after birth, once daily for 7 consecutive days. All rat pups were killed by decapitation on day 21 after birth, and then 14-3-3, Bcl-2 and Bax immunoactivity (expression) in the cerebral cortex were detected by immunohistochemistry.
  
  
  RESULTS
  In comparison with the normal group, the expression level of cerebral cortical 14-3-3 was significantly decreased, and that of Bax remarkably increased in the model group (P<0. 01, P<0. 05). Compared to the model group, cortical 14-3-3 and Bcl-2 expression levels were markedly up-regulated in the acupuncture group (P<0.01, P<0.05). Compared to the normal group, cortical 14-3-3 expression level was obviously lower, but Bax expression level significantly higher in the sham-operation group (P<0. 05, P<0. 01). No significant differences were found between the model and normal groups in the expression levels of Bcl-2, and between the acupuncture and model groups in the expression levels of Bax (P>0. 05).
  
  
  CONCLUSIONS
  Acupuncture intervention can increase the expression of 14-3-3 and Bcl-2 in the cerebral cortex in HIBD rats.
authors: Xing-er
authors: XE
authors: Li
authors: Qing
authors: Q
authors: Yuan
authors: Chun-zhi
authors: CZ
authors: Tang
authors: Fei
authors: F
authors: Chen
authors: Rong
authors: R
authors: Zhao
authors: Long-lin
authors: LL
authors: Liu
authors: Yu-tian
authors: YT
authors: Yu
authors: Yong
authors: Y
authors: Cao
authors: Jia-li
authors: JL
authors: Wu
authors: Shuo
authors: S
authors: Sun
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis*; D015670:Acupuncture Therapy; D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000628:therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: D048948:14-3-3 Proteins; C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein