title: Glucose-6-phosphate tips the balance in modulating apoptosis in cerebellar granule cells.
abstract: A metabolic shift from oxidative phosphorylation to glycolysis (i.e. the Warburg effect) occurs in Alzheimer's disease accompanied by an increase of both activity and level of HK-I. The findings reported here demonstrate that in the early phase of apoptosis VDAC1 activity, but not its protein level, progressively decreases, in concomitance with the physical interaction of HK-I with VDAC1. In the late phase of apoptosis, glucose-6-phosphate accumulation in the cell causes the dissociation of the two proteins, the re-opening of the channel and the recovery of VDAC1 function, resulting in a reawakening of the mitochondrial function, thus inevitably leading to cell death.
authors: Institute of Biomembrane and Bioenergetics (IBBE) - CNR, Via Amendola, 165/A, 70126 Bari, Italy.
authors: A
authors: A
authors: Bobba
authors: Institute of Translational Pharmacology (IFT) - CNR, Via Fosso del Cavaliere, 100, 00133 Rome, Italy.
authors: G
authors: G
authors: Amadoro
authors: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Via Orabona, 4, 70126 Bari, Italy.
authors: G
authors: G
authors: La Piana
authors: Institute of Biomembrane and Bioenergetics (IBBE) - CNR, Via Amendola, 165/A, 70126 Bari, Italy.
authors: V A
authors: VA
authors: Petragallo
authors: European Brain Research Institute (EBRI), Via del Fosso di Fiorano, 64-65, 00143 Rome, Italy.
authors: P
authors: P
authors: Calissano
authors: Institute of Biomembrane and Bioenergetics (IBBE) - CNR, Via Amendola, 165/A, 70126 Bari, Italy. Electronic address: a.atlante@ibbe.cnr.it.
authors: A
authors: A
authors: Atlante
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008928:Mitochondria / Q000378:metabolism; D011485:Protein Binding; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: C494762:VDAC1 protein, human; D019298:Glucose-6-Phosphate; D050995:Voltage-Dependent Anion Channel 1; D006593:Hexokinase
keywords: Apoptosis; Glucose-6-phosphate; Hexokinase; Mitochondria; Voltage-dependent anion channel