title: Profibrosing effect of angiotensin converting enzyme inhibitors in human lung fibroblasts.
abstract: OBJECTIVE
  The objective of this study is to determine the effect of two angiotensin-converting enzyme inhibitors (ACEi) (Enalapril and Captopril), an angiotensin-II receptor inhibitor (Losartan) and a renin inhibitor (Aliskiren) on renin, TGF-β1 and collagen expressions in human lung fibroblast cultures through real-time PCR and ELISA.
  
  
  METHODS
  Normal commercial fibroblasts (CCD25) were exposed to 10(-6) M of enalapril, captopril, losartan, or aliskiren for 6 h. Subsequently, media were recovered and proteins were concentrated; RNA was extracted from the cells. Real time-PCR and ELISA were performed.
  
  
  RESULTS
  ACEi and losartan-stimulated fibroblasts showed an increase in the expression of TGF-β1, Collagen-Iα1 (Col-Iα1), and renin (except losartan) vs PolR2A (p < 0.05), and upregulation of TGF-β1 protein (p < 0.01), except with aliskiren.
  
  
  CONCLUSIONS
  Results show that ACEis and losartan could play a profibrosing role by inducing the overexpression of molecules such TGF-β1 and Collagen.
authors: Departamento de Biología Molecular, Instituto Nacional de Enfermedades Respiratorias,"Ismael Cosío Villegas", Calz. Tlalpan 4502, Col. Sección XVI, 14080, D.F., Mexico.
authors: Gabriela
authors: G
authors: Díaz-Piña
authors: Eduardo
authors: E
authors: Montes
authors: Marco
authors: M
authors: Checa
authors: Carina
authors: C
authors: Becerril
authors: Carolina
authors: C
authors: García de Alba
authors: Anita
authors: A
authors: Vega
authors: Ignacio
authors: I
authors: Páramo
authors: Rosa
authors: R
authors: Ordoñez-Razo
authors: Victor
authors: V
authors: Ruiz
mesh_terms: D000577:Amides / Q000494:pharmacology; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D002216:Captopril / Q000494:pharmacology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D004656:Enalapril / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D005355:Fibrosis; D005650:Fumarates / Q000494:pharmacology; D006801:Humans; D019808:Losartan / Q000494:pharmacology; D008168:Lung / Q000473:pathology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012083:Renin / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D000092502:Renin Inhibitors
chemical_list: D000577:Amides; D047228:Angiotensin II Type 1 Receptor Blockers; D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D004656:Enalapril; D005650:Fumarates; D019808:Losartan; D012083:Renin; D053773:Transforming Growth Factor beta1; D000092502:Renin Inhibitors; C446481:aliskiren
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