title: Relationship of light scatter change and Cdc42-regulated actin status.
abstract: Cdc42 GTPase has important roles in regulating intracellular actin reorganization. The current methods to monitor actin changes are typically complex and point by point.
  
  
  
  The effects of Cdc42 inhibitors on the side scatter changes were tested in a newly developed continuous assay using the flow cytometer. Staining with fluorescently labeled phalloidin was used for comparison.
  
  
  
  Cdc42-specific inhibitors caused dose-dependent changes of both the right-angle side scatter and the phalloidin-stained actin.
  
  
  
  The right-angle light scatter change can be used as a method to circumvent phalloidin staining and be an early convenient step in screening Cdc42 inhibitors. © 2015 International Clinical Cytometry Society.
authors: Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.|Department of Pathology, Center for Molecular Discovery, The University of New Mexico, Albuquerque, New Mexico, 87131.
authors: Lin
authors: L
authors: Hong
authors: Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.
authors: Stephanie
authors: S
authors: Chavez
authors: Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.
authors: Yelena
authors: Y
authors: Smagley
authors: Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.
authors: Alexandre
authors: A
authors: Chigaev
authors: Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.|Department of Pathology, Center for Molecular Discovery, The University of New Mexico, Albuquerque, New Mexico, 87131.|Cancer Research and Treatment Center, The University of New Mexico, Albuquerque, New Mexico, 87131.
authors: Larry A
authors: LA
authors: Sklar
mesh_terms: D000199:Actins / Q000378:metabolism*; D000067493:Dynamic Light Scattering / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D020764:cdc42 GTP-Binding Protein
keywords: Cdc42; actin; phalloidin; polymerization; side scatter
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