title: A putative porin gene of Burkholderia sp. NK8 involved in chemotaxis toward β-ketoadipate.
abstract: Burkholderia sp. NK8 can utilize 3-chlorobenzoate (3CB) as a sole source of carbon because it has a megaplasmid (pNK8) that carries the gene cluster (tfdT-CDEF) encoding chlorocatechol-degrading enzymes. The expression of tfdT-CDEF is induced by 3CB. In this study, we found that NK8 cells were attracted to 3CB and its degradation products, 3- and 4-chlorocatechol, and β-ketoadipate. Capillary assays revealed that a pNK8-eliminated strain (NK82) was defective in chemotaxis toward β-ketoadipate. The introduction of a plasmid carrying a putative outer membrane porin gene, which we name ompNK8, into strain NK82 restored chemotaxis toward β-ketoadipate. RT-PCR analyses demonstrated that the transcription of the ompNK8 gene was enhanced in the presence of 3CB.
authors: a Environmental Biofunction Division , National Institute for Agro-Environmental Sciences , Tsukuba , Japan.
authors: Kimiko
authors: K
authors: Yamamoto-Tamura
authors: Ikuro
authors: I
authors: Kawagishi
authors: Naoto
authors: N
authors: Ogawa
authors: Takeshi
authors: T
authors: Fujii
mesh_terms: D000272:Adipates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000166:cytology* / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects; D002723:Chlorobenzoates / Q000378:metabolism / Q000633:toxicity; D007700:Kinetics; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D018272:Porins / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000272:Adipates; D001426:Bacterial Proteins; D002723:Chlorobenzoates; D018272:Porins; C036427:3-chlorobenzoic acid; C027316:3-oxoadipic acid
keywords: 3-chlorobenzoate; chemoattractant; chloroaromatic compounds; porin