title: The conformational plasticity of eukaryotic RNA-dependent ATPases.
abstract: RNA helicases are present in all domains of life and participate in almost all aspects of RNA metabolism, from transcription and processing to translation and decay. The diversity of pathways and substrates that they act on is reflected in the diversity of their individual functions, structures, and mechanisms. However, RNA helicases also share hallmark properties. At the functional level, they promote rearrangements of RNAs and RNP particles by coupling nucleic acid binding and release with ATP hydrolysis. At the molecular level, they contain two domains homologous to the bacterial RecA recombination protein. This conserved catalytic core is flanked by additional domains, which typically regulate the ATPase activity in cis. Binding to effector proteins targets or regulates the ATPase activity in trans. Structural and biochemical studies have converged on the plasticity of RNA helicases as a fundamental property that is used to control their timely activation in the cell. In this review, we focus on the conformational regulation of conserved eukaryotic RNA helicases.
authors: Structural Cell Biology Department, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors: Sevim
authors: S
authors: Ozgur
authors: Gretel
authors: G
authors: Buchwald
authors: Sebastian
authors: S
authors: Falk
authors: Sutapa
authors: S
authors: Chakrabarti
authors: Jesuraj Rajan
authors: JR
authors: Prabu
authors: Elena
authors: E
authors: Conti
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000378:metabolism; D005057:Eukaryotic Cells / Q000201:enzymology; D008958:Models, Molecular; D011487:Protein Conformation; D020365:RNA Helicases / Q000737:chemistry* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D055672:Static Electricity
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C074151:NAM7 protein, S cerevisiae; C027900:RNA-dependent ATPase; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases
keywords: DEAD-box protein; DExH-box protein; RNA helicase; RNA-dependent ATPase; RNA-protein interaction; SF1; SF2