title: Bacterial autoinducer-2 detection via an engineered quorum sensing protein.
abstract: Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
authors: Department of Chemistry, University of Kentucky , Lexington, Kentucky 40506, United States.
authors: Nilesh
authors: N
authors: Raut
authors: Smita
authors: S
authors: Joel
authors: Patrizia
authors: P
authors: Pasini
authors: Sylvia
authors: S
authors: Daunert
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003374:Coumarins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D064368:Healthy Volunteers; D006714:Homoserine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007783:Lactones / Q000032:analysis*; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D053038:Quorum Sensing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003374:Coumarins; D007783:Lactones; C090749:LuxP protein, Vibrio; C091088:N-octanoylhomoserine lactone; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D006714:Homoserine; C030123:coumarin