title: Curcumin suppresses invasiveness and vasculogenic mimicry of squamous cell carcinoma of the larynx through the inhibition of JAK-2/STAT-3 signaling pathway.
abstract: To determine the role of JAK-2/STAT-3 signaling pathway in invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma. HEp-2 cells were treated with 1 or 10 μmol/L curcumin and AG490 (the inhibitor of JAK-2) for 48 h, the invasion and vasculogenic mimicry of tumor cells were tested with Transwell chamber test and tube formation experiment. RT-PCR was used to measure the expression of MMP-2 and VEGF. Western blot assay was employed to determine the expression of JAK-2, STAT3, p-STAT3, MMP-2 and VEGF. Compared to control group，there were less tumor cells permeating membrane and less formed tubes after curcumin or AG490 treatment, RT-PCR showed that the expression of MMP-2 and VEGF at mRNA level were decreased (P < 0.01). Western blotting indicated that the expression of JAK-2, p-STAT3, MMP-2 and VEGF at protein levels were decreased (P < 0.01), while that of STAT-3 protein had no difference among each group (P > 0.05). Immunofluorescence staining demonstrated that the expression of eNOS was down-regulated (P < 0.01). Curcumin and AG490 significantly inhibits invasion and vasculogenic mimicry of laryngeal squamous cell carcinoma in vitro, and JAK-2/STAT-3 signaling pathway promotes above processes.
authors: Department of Otolaryngology, Gongli Hospital Pudong New Area, Miaopu Road 219, Shanghai 200135, China.
authors: An
authors: A
authors: Hu
authors: Department of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Kongjiang Road 1665, Shanghai 200092, China.
authors: Jing-Juan
authors: JJ
authors: Huang
authors: Department of Otolaryngology Head and Neck Surgery, Renji Hospital, Pudong New Area, Dongfang Road 1630, Shanghai Jiaotong University School of Medicine Shanghai 200127, China.
authors: Xiao-Jie
authors: XJ
authors: Jin
authors: Department of Otolaryngology Head and Neck Surgery, Renji Hospital, Pudong New Area, Dongfang Road 1630, Shanghai Jiaotong University School of Medicine Shanghai 200127, China.
authors: Ji-Ping
authors: JP
authors: Li
authors: Joint Molecular Rheumatology Laboratory of The Institute of Health Sciences and Shanghai Renji Hospital, Shanghai JiaoTong University School of Medicine, and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shandong(M) Road 145, Shanghai 200001, China.
authors: Yuan-Jia
authors: YJ
authors: Tang
authors: Joint Molecular Rheumatology Laboratory of The Institute of Health Sciences and Shanghai Renji Hospital, Shanghai JiaoTong University School of Medicine, and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shandong(M) Road 145, Shanghai 200001, China.
authors: Xin-Fang
authors: XF
authors: Huang
authors: Joint Molecular Rheumatology Laboratory of The Institute of Health Sciences and Shanghai Renji Hospital, Shanghai JiaoTong University School of Medicine, and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shandong(M) Road 145, Shanghai 200001, China.
authors: Hui-Juan
authors: HJ
authors: Cui
authors: Department of Otolaryngology, Gongli Hospital Pudong New Area, Miaopu Road 219, Shanghai 200135, China.
authors: Wei-Hua
authors: WH
authors: Xu
authors: Department of Otolaryngology Head and Neck Surgery, Huashan Hospital, Shanghai Fudan University School of Medicine Wurumuqi Road 12, Shanghai 200040, China.
authors: Guang-Bin
authors: GB
authors: Sun
keywords: JAK-2/STAT-3 signaling pathway; Laryngeal squamous cell carcinoma; curcumin; vasculogenic mimicry
references: Mutat Res. 1999 Jul 16;428(1-2):305-27
references: 10518003
references: Am J Cancer Res. 2014 Sep 06;4(5):484-94
references: 25232490
references: Circ Res. 2002 Nov 1;91(9):837-44
references: 12411399
references: Nat Rev Cancer. 2002 Oct;2(10):740-9
references: 12360277
references: Int J Cancer. 2015 Mar 15;136(6):E545-58
references: 25284767
references: J Exp Clin Cancer Res. 2010 Jun 02;29:60
references: 20525189
references: Biomed Res Int. 2014;2014:832562
references: 24999479
references: Semin Oncol. 2007 Apr;34(2 Suppl 1):S37-40
references: 17449351
references: J Surg Oncol. 2013 Nov;108(6):414-9
references: 23996537
references: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references: 15761078
references: Gastroenterology. 1999 Dec;117(6):1278-87
references: 10579968
references: Gastroenterology. 1999 Dec;117(6):1271-7
references: 10579967
references: Biochimie. 2014 Sep;104:117-26
references: 24950183
references: Am J Obstet Gynecol. 2004 Apr;190(4):899-909
references: 15118611
references: Am J Pathol. 1999 Sep;155(3):739-52
references: 10487832
references: Ann Oncol. 2005 Mar;16(3):481-8
references: 15718248
references: Oncol Lett. 2014 Mar;7(3):755-763
references: 24520293
references: Oncogene. 2005 Feb 10;24(7):1188-202
references: 15558015
references: Carcinogenesis. 1995 Oct;16(10 ):2493-7
references: 7586157
references: Gut. 2006 Oct;55(10):1497-511
references: 16966704
references: Am J Cancer Res. 2011;1(6):806-16
references: 22016828
references: Anticancer Res. 2014 Apr;34(4):1857-64
references: 24692720
references: Circ Res. 2001 Sep 14;89(6):477-9
references: 11557733
references: Biochim Biophys Acta. 2013 Jan;1835(1):46-60
references: 23103770
references: Nat Med. 2005 Jun;11(6):623-9
references: 15895073
references: Am J Pathol. 2012 Oct;181(4):1115-25
references: 22944600
references: Cancer Res. 2003 Jun 1;63(11):2812-9
references: 12782586
references: Am J Pathol. 2000 Feb;156(2):361-81
references: 10666364
references: Gynecol Oncol. 2014 Jun;133(3):575-83
references: 24589413
references: Mol Cancer. 2014 Sep 08;13:207
references: 25200065
references: Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):8018-23
references: 11416160
references: Am J Cancer Res. 2013 Jun 20;3(3):302-11
references: 23841029
references: N Engl J Med. 2006 Sep 21;355(12):1222-32
references: 16990385
references: J Gastrointest Surg. 2013 May;17(5):887-98
references: 23435739
references: PLoS One. 2013 Jul 09;8(7):e68391
references: 23950841
references: Oral Oncol. 2011 Dec;47(12):1154-8
references: 21873105