title: Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection.
abstract: Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective "turn on" plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of ~0.1nM (200amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination.
  
  
  BACKGROUND
  Surface-enhanced Raman scattering (SERS) is a sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces. The enhancement means that the technique may even detect single molecules. In this article, the authors describe a simple and efficient nucleic acid detection technology using SERS, with "OFF-to-ON" signal switch upon nucleic acid target identification and capture, which provides high sensitivity and specificity for single nucleotide mismatch discrimination. This new technology will be most welcomed in clinical diagnostics.
authors: Department of Biomedical Engineering, Duke University, Durham, NC, USA; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA.
authors: Hsin-Neng
authors: HN
authors: Wang
authors: Department of Biomedical Engineering, Duke University, Durham, NC, USA; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA.
authors: Andrew M
authors: AM
authors: Fales
authors: Department of Biomedical Engineering, Duke University, Durham, NC, USA; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA; Department of Chemistry, Duke University, Durham, NC, USA. Electronic address: tuan.vodinh@duke.edu.
authors: Tuan
authors: T
authors: Vo-Dinh
mesh_terms: D004247:DNA / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D004247:DNA
keywords: Nanobiosensor; Nucleic acid detection; Surface-enhanced Raman scattering (SERS)