title: Lost in transcription: transient errors in information transfer.
abstract: Errors in information transfer from DNA to RNA to protein are inevitable. Here, we focus on errors that occur in nascent transcripts during transcription, epimutations. Recent approaches using novel cDNA library preparation and next-generation sequencing begin to directly determine the rate of epimutation and allow analysis of the epimutational spectrum of transcription errors, the type and sequence context of the errors produced in a transcript by an RNA polymerase. The phenotypic consequences of transcription errors have been assessed using both forward and reverse epimutation systems. These studies reveal that transient transcription errors can produce a modification of cell phenotype, partial phenotypic suppression of a mutant allele, and a heritable change in cell phenotype, epigenetic switching in a bistable gene network.
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors: Alasdair J E
authors: AJ
authors: Gordon
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors: Dominik
authors: D
authors: Satory
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors: Jennifer A
authors: JA
authors: Halliday
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA; Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: herman@bcm.edu.
authors: Christophe
authors: C
authors: Herman
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D044127:Epigenesis, Genetic; D004926:Escherichia coli / Q000235:genetics*; D053263:Gene Regulatory Networks; D009154:Mutation; D010641:Phenotype; D012322:RNA Precursors / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D012322:RNA Precursors; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases
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