title: Ligand-directed profiling of organelles with internalizing phage libraries.
abstract: Phage display is a resourceful tool to, in an unbiased manner, discover and characterize functional protein-protein interactions, create vaccines, and engineer peptides, antibodies, and other proteins as targeted diagnostic and/or therapeutic agents. Recently, our group has developed a new class of internalizing phage (iPhage) for ligand-directed targeting of organelles and to identify molecular pathways within live cells. This unique technology is suitable for applications ranging from fundamental cell biology to drug development. This unit describes the methods for generating and screening the iPhage display system, and explains how to select and validate candidate internalizing homing peptide.
authors: Division of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.|Division of Molecular Medicine, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.|These authors contributed equally to this work.
authors: Andrey S
authors: AS
authors: Dobroff
authors: Cancer Research Program, Houston Methodist Research Institute, Houston, Texas.|These authors contributed equally to this work.
authors: Roberto
authors: R
authors: Rangel
authors: Cancer Research Program, Houston Methodist Research Institute, Houston, Texas.|These authors contributed equally to this work.
authors: Liliana
authors: L
authors: Guzman-Roja
authors: Division of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.|Division of Molecular Medicine, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
authors: Carolina C
authors: CC
authors: Salmeron
authors: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan.
authors: Juri G
authors: JG
authors: Gelovani
authors: Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts.
authors: Richard L
authors: RL
authors: Sidman
authors: Translational Informatics Division, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
authors: Cristian G
authors: CG
authors: Bologa
authors: Translational Informatics Division, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
authors: Tudor I
authors: TI
authors: Oprea
authors: Department of Chemical and Nuclear Engineering, The University of New Mexico Cancer Center, Albuquerque, New Mexico.
authors: C Jeffrey
authors: CJ
authors: Brinker
authors: Division of Molecular Medicine, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.|These authors contributed equally as senior authors to this work.
authors: Renata
authors: R
authors: Pasqualini
authors: Division of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.|These authors contributed equally as senior authors to this work.
authors: Wadih
authors: W
authors: Arap
mesh_terms: D045744:Cell Line, Tumor; D061505:Cell Surface Display Techniques / Q000379:methods*; D003001:Cloning, Molecular; D003584:Cytological Techniques / Q000379:methods*; D004926:Escherichia coli; D006801:Humans; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism*
keywords: intracellular receptors; intracellular targeting; mammalian cells; organelles; penetratin; peptides; phage display; proteomics
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