title: The SWI/SNF chromatin remodeling complex regulates germinal center formation by repressing Blimp-1 expression.
abstract: Germinal center (GC) reaction is crucial in adaptive immune responses. The formation of GC is coordinated by the expression of specific genes including Blimp-1 and Bcl-6. Although gene expression is critically influenced by the status of chromatin structure, little is known about the role of chromatin remodeling factors for regulation of GC formation. Here, we show that the SWI/SNF chromatin remodeling complex is required for GC reactions. Mice lacking Srg3/mBaf155, a core component of the SWI/SNF complex, showed impaired differentiation of GC B and follicular helper T cells in response to T cell-dependent antigen challenge. The SWI/SNF complex regulates chromatin structure at the Blimp-1 locus and represses its expression by interacting cooperatively with Bcl-6 and corepressors. The defect in GC reactions in mice lacking Srg3 was due to the derepression of Blimp-1 as supported by genetic studies with Blimp-1-ablated mice. Hence, our study identifies the SWI/SNF complex as a key mediator in GC reactions by modulating Bcl-6-dependent Blimp-1 repression.
authors: School of Biological Sciences and Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea.
authors: Jinwook
authors: J
authors: Choi
authors: School of Biological Sciences and Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea.
authors: Shin
authors: S
authors: Jeon
authors: School of Biological Sciences and Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea.
authors: Seungjin
authors: S
authors: Choi
authors: School of Biological Sciences and Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea.
authors: Kyungsoo
authors: K
authors: Park
authors: School of Biological Sciences and Institute for Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea rhseong@snu.ac.kr.
authors: Rho Hyun
authors: RH
authors: Seong
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002843:Chromatin / Q000737:chemistry; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D018858:Germinal Center / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011487:Protein Conformation; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C513258:Prdm1 protein, mouse; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1
keywords: Blimp-1; SWI/SNF chromatin remodeling complex; follicular helper T cell; germinal center reaction
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