title: Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
abstract: The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria. Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv. Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting). Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs). The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine. The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
authors: Institute of Bioanalysis and Szentágothai Research Centre, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Lilla
authors: L
authors: Makszin
authors: Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Zoltán
authors: Z
authors: Péterfi
authors: Institute of Bioanalysis and Szentágothai Research Centre, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Ágnes
authors: Á
authors: Blaskó
authors: Institute of Bioanalysis and Szentágothai Research Centre, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Viktor
authors: V
authors: Sándor
authors: MTA-PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary.
authors: Anikó
authors: A
authors: Kilár
authors: Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Ágnes
authors: Á
authors: Dörnyei
authors: Department of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Erzsébet
authors: E
authors: Ősz
authors: Institute of Bioanalysis and Szentágothai Research Centre, Faculty of Medicine, University of Pécs, Pécs, Hungary.|Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Ferenc
authors: F
authors: Kilár
authors: Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors: Béla
authors: B
authors: Kocsis
mesh_terms: D002236:Carbohydrate Conformation; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000276:immunology*; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D008070:Lipopolysaccharides
keywords: Colitose; Escherichia coli O111; Microchip electrophoresis; Proteus morganii O34; Salmonella enterica sv. Adelaide O35