title: Tuberculous spondylitis in Russia and prominent role of multidrug-resistant clone Mycobacterium tuberculosis Beijing B0/W148.
abstract: Extrapulmonary and, in particular, spinal tuberculosis (TB) constitutes a minor but significant part of the total TB incidence. In spite of this, almost no studies on the genetic diversity and drug resistance of Mycobacterium tuberculosis isolates from spinal TB patients have been published to date. Here, we report results of the first Russian and globally largest molecular study of M. tuberculosis isolates recovered from patients with tuberculous spondylitis (TBS). The majority of 107 isolates were assigned to the Beijing genotype (n = 80); the other main families were T (n = 11), Ural (n = 7), and LAM (n = 4). Multidrug resistance (MDR) was more frequently found among Beijing (90.5%) and, intriguingly, Ural (71.4%) isolates than other genotypes (5%; P < 0.001). The extremely drug-resistant (XDR) phenotype was exclusively found in the Beijing isolates (n = 7). A notable prevalence of the rpoB531 and katG315 mutations in Beijing strains that were similarly high in both TBS (this study) and published pulmonary TB (PTB) samples from Russia shows that TBS and PTB Beijing strains follow the same paradigm of acquisition of rifampin (RIF) and isoniazid (INH) resistance. The 24-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) subtyping of 80 Beijing isolates further discriminated them into 24 types (Hunter Gaston index [HGI] = 0.83); types 100-32 and 94-32 represented the largest groups. A genotype of Russian successful clone B0/W148 was identified in 30 of 80 Beijing isolates. In conclusion, this study highlighted a crucial impact of the Beijing genotype and the especially prominent role of its MDR-associated successful clone B0/W148 cluster in the development of spinal MDR-TB in Russian patients.
authors: Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia annavyazovaya@gmail.com igormokrousov@yahoo.com.
authors: Anna
authors: A
authors: Vyazovaya
authors: Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia annavyazovaya@gmail.com igormokrousov@yahoo.com.
authors: Igor
authors: I
authors: Mokrousov
authors: Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Natalia
authors: N
authors: Solovieva
authors: Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Alexander
authors: A
authors: Mushkin
authors: Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Olga
authors: O
authors: Manicheva
authors: Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Boris
authors: B
authors: Vishnevsky
authors: Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Viacheslav
authors: V
authors: Zhuravlev
authors: Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia Research Institute of Phthisiopulmonology, St. Petersburg, Russia.
authors: Olga
authors: O
authors: Narvskaya
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D018598:Minisatellite Repeats; D009154:Mutation / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D015995:Prevalence; D012293:Rifampin / Q000494:pharmacology; D012426:Russia / Q000453:epidemiology; D013166:Spondylitis / Q000453:epidemiology / Q000382:microbiology*; D014399:Tuberculosis, Spinal / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin
references: J Clin Microbiol. 2006 Jul;44(7):2378-81
references: 16825352
references: Tuberculosis (Edinb). 2013 Jan;93(1):75-83
references: 23140853
references: Clin Microbiol Infect. 2005 Jul;11(7):531-9
references: 15966970
references: J Clin Microbiol. 1993 Feb;31(2):406-9
references: 8381814
references: J Clin Microbiol. 2005 Mar;43(3):1390-4
references: 15750115
references: J Clin Microbiol. 2006 Dec;44(12):4498-510
references: 17005759
references: Clin Exp Immunol. 2003 Jul;133(1):30-7
references: 12823275
references: Mem Inst Oswaldo Cruz. 2011 Aug;106(5):524-35
references: 21894371
references: J Bacteriol. 2014 May;196(10):1833-41
references: 24584500
references: Wkly Epidemiol Rec. 2006 Nov 10;81(45):430-2
references: 17096498
references: Probl Tuberk. 1999;(3):39-41
references: 10420767
references: Asian Spine J. 2012 Dec;6(4):294-308
references: 23275816
references: J Microbiol Immunol Infect. 2013 Feb;46(1):19-23
references: 22575429
references: BMC Microbiol. 2012;12:90
references: 22646308
references: J Clin Microbiol. 1997 Apr;35(4):907-14
references: 9157152
references: Infect Genet Evol. 2012 Jun;12(4):619-29
references: 22036706
references: Infect Genet Evol. 2012 Jun;12(4):642-8
references: 21896337
references: Probl Tuberk Bolezn Legk. 2006;(11):3-6
references: 17195581
references: J Clin Microbiol. 2012 Nov;50(11):3757-9
references: 22933595
references: J Clin Microbiol. 2008 Apr;46(4):1363-8
references: 18287322
references: J Spinal Cord Med. 2011;34(5):440-54
references: 22118251
references: J Clin Microbiol. 2008 Apr;46(4):1259-67
references: 18234868
references: Infect Genet Evol. 2008 Dec;8(6):777-85
references: 18691674
references: Euro Surveill. 2013;18(12). pii: 20432
references: 23557946
references: Clin Microbiol Rev. 2013 Apr;26(2):342-60
references: 23554420
references: Infect Genet Evol. 2006 Jan;6(1):13-21
references: 16376836
references: PLoS One. 2009;4(2):e4540
references: 19234602
references: Infect Genet Evol. 2012 Jun;12(4):755-66
references: 22365971