title: iTRAQ-Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is a common disease in the southern provinces of China with a poor prognosis. To better understand the pathogenesis of NPC and identify proteins involved in NPC carcinogenesis, we applied iTRAQ coupled with two-dimensional LC-MS/MS to compare the proteome profiles of NPC tissues and the adjacent non-tumor tissues. We identified 54 proteins with differential expression in NPC and the adjacent non-tumor tissues. The differentially expressed proteins were further determined by RT-PCR and Western blot analysis. In addition, the up-regulation of HSPB1, NPM1 and NCL were determined by immunohistochemistry using tissue microarray. Functionally, we found that siRNA mediated knockdown of NPM1 inhibited the migration and invasion of human NPC CNE1 cell line. In summary, this is the first study on proteome analysis of NPC tissues using an iTRAQ method, and we identified many new differentially expressed proteins which are potential targets for the diagnosis and therapy of NPC.
authors: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, China.
authors: Xin-Zhang
authors: XZ
authors: Cai
authors: Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.|Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Wei-Qun
authors: WQ
authors: Zeng
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Yi
authors: Y
authors: Xiang
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Yi
authors: Y
authors: Liu
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Hong-Min
authors: HM
authors: Zhang
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Hong
authors: H
authors: Li
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Sha
authors: S
authors: She
authors: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Min
authors: M
authors: Yang
authors: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, China.
authors: Kun
authors: K
authors: Xia
authors: Department of Hepatology and Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Department of Health Management Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors: Shi-Fang
authors: SF
authors: Peng
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000097796:Nucleolin
chemical_list: D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C057725:HSPB1 protein, human; C000716967:NPM1 protein, human
keywords: NASOPHARYNGEAL CARCINOMA; PROTEOME; iTRAQ