title: The histone demethylase Jarid1b is required for hematopoietic stem cell self-renewal in mice.
abstract: Jarid1b/KDM5b is a histone demethylase that regulates self-renewal and differentiation in stem cells and cancer; however, its function in hematopoiesis is unclear. Here, we find that Jarid1b is highly expressed in primitive hematopoietic compartments and is overexpressed in acute myeloid leukemias. Constitutive genetic deletion of Jarid1b did not impact steady-state hematopoiesis. In contrast, acute deletion of Jarid1b from bone marrow increased peripheral blood T cells and, following secondary transplantation, resulted in loss of bone marrow reconstitution. Our results reveal that deletion of Jarid1b compromises hematopoietic stem cell (HSC) self-renewal capacity and suggest that Jarid1b is a positive regulator of HSC potential.
authors: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA; Department of Stem Cell and Regenerative Medicine, Harvard University, Cambridge, MA;
authors: Morag H
authors: MH
authors: Stewart
authors: Biotech Research and Innovation Center, Center for Epigenetics, and The Danish Stem Cell Center, University of Copenhagen, Copenhagen, Denmark; and.
authors: Mareike
authors: M
authors: Albert
authors: Biotech Research and Innovation Center, Center for Epigenetics, and The Danish Stem Cell Center, University of Copenhagen, Copenhagen, Denmark; and.
authors: Patrycja
authors: P
authors: Sroczynska
authors: Biotech Research and Innovation Center, Center for Epigenetics, and The Danish Stem Cell Center, University of Copenhagen, Copenhagen, Denmark; and.
authors: V Adam
authors: VA
authors: Cruickshank
authors: Stem Cell Group, University College London Cancer Institute, University College London, London, United Kingdom.
authors: Yanping
authors: Y
authors: Guo
authors: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA; Department of Stem Cell and Regenerative Medicine, Harvard University, Cambridge, MA;
authors: Derrick J
authors: DJ
authors: Rossi
authors: Biotech Research and Innovation Center, Center for Epigenetics, and The Danish Stem Cell Center, University of Copenhagen, Copenhagen, Denmark; and.
authors: Kristian
authors: K
authors: Helin
authors: Stem Cell Group, University College London Cancer Institute, University College London, London, United Kingdom.
authors: Tariq
authors: T
authors: Enver
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout
chemical_list: D004268:DNA-Binding Proteins; D056484:Jumonji Domain-Containing Histone Demethylases; C485614:Kdm5b protein, mouse
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