title: Diversity of antimicrobial resistance and virulence genes in methicillin-resistant non-Staphylococcus aureus staphylococci from veal calves.
abstract: In this study we determined whether methicillin-resistant non-Staphylococcus aureus (MRNAS) from veal calves may be a potential reservoir of antimicrobial-resistance and virulence genes. Fifty-eight MRNAS were studied by means of DNA-microarray and PCR for detection of antimicrobial resistance and virulence genes. The isolates carried a variety of antimicrobial-resistance genes [aacA-aphD, aadD, aph3, aadE, sat, spc, ampA, erm(A), erm(B), erm(C), erm(F), erm(T), lnu(A), msr(A)-msr(B), vga(A), mph(C), tet(K), tet(M), tet(L), cat, fexA, dfrA, dfrD, dfrG, dfrK, cfr, fusB, fosB, qacA, qacC, merA-merB]. Some isolates carried resistance genes without showing the corresponding resistance phenotype. Most MRNAS carried typical S. aureus virulence factors like proteases (sspP) and enterotoxins (seg) genes. Most Staphylococcus epidermidis isolates carried the arginine catabolic element, and nearly 40% of the Staphylococcus sciuri isolates carried leukocidins, and/or fibronectin-binding protein genes. MRNAS were highly multi-resistant and represent an important reservoir of antimicrobial resistance and virulence genes.
authors: Department of Bacterial Diseases, Veterinary and Agrochemical Research Centre, Groeselenbergstraat 99, B-1180 Ukkel, Brussels, Belgium. Electronic address: maria.argudin@erasme.ulb.ac.be.
authors: M Angeles
authors: MA
authors: Argudín
authors: Department of Obstetrics, Reproduction and Herd Health, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors: Wannes
authors: W
authors: Vanderhaeghen
authors: Department of Bacterial Diseases, Veterinary and Agrochemical Research Centre, Groeselenbergstraat 99, B-1180 Ukkel, Brussels, Belgium; Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors: Patrick
authors: P
authors: Butaye
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000235:genetics* / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors
keywords: Coagulase negative staphylococci; Epidemiology; Zoonotic