title: The masquelet induced membrane technique with BMP and a synthetic scaffold can heal a rat femoral critical size defect.
abstract: Long bone defects can be managed by the induced membrane technique together with autologous bone graft. However, graft harvest is associated with donor site morbidity. This study investigates if a tricalcium phosphate hydroxyapatite scaffold can be used alone or in combination with bone active drugs to improve healing. Sprague Dawley rats (n = 40) were randomized into four groups. (A) scaffold, (B) BMP-7, (C) BMP-7 + scaffold, and (D) BMP-7 + scaffold + systemic bisphosphonate at 2 weeks. Locked femoral nailing was followed by 6 mm segment removal and implantation of an epoxy spacer. At 4 weeks, the spacers were removed and the defects grafted. Eleven weeks later, the bones were explanted for evaluation with radiography, manual assessment, micro-CT, histology, and Fourier Transform Infrared spectroscopy (FTIR). Isolated scaffolds (A) did not heal any defects, whereas the other treatments led to healing in 7/10 (B), 10/10 (C), and 9/10 (D) rats. Group D had greater volume of highly mineralized bone (p < 0.01) and higher bone volume fraction (p < 0.01) compared to all other groups. A synthetic scaffold + BMP-7 combined with a bisphosphonate improved the callus properties in a rat femoral critical size defect, compared to both BMP-7 and scaffold alone or the two combined.
authors: Department of Orthopaedics, Clinical Sciences, Lund University, Lund, Sweden.
authors: Per
authors: P
authors: Bosemark
authors: Christina
authors: C
authors: Perdikouri
authors: Mea
authors: M
authors: Pelkonen
authors: Hanna
authors: H
authors: Isaksson
authors: Magnus
authors: M
authors: Tägil
mesh_terms: D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism / Q000494:pharmacology; D016025:Bone Transplantation / Q000379:methods*; D002146:Bony Callus / Q000187:drug effects; D018483:Diaphyses / Q000293:injuries*; D004164:Diphosphonates / Q000494:pharmacology; D005269:Femur / Q000000981:diagnostic imaging / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D008297:Male; D011859:Radiography; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000628:therapy*
chemical_list: C525818:Bmp6 protein, rat; D055418:Bone Morphogenetic Protein 6; D004164:Diphosphonates
keywords: BMP-7; Masquelet; bisphosphonate; critical size defect; induced membrane