title: Palladium uptake by Pisum sativum: partitioning and effects on growth and reproduction.
abstract: Environmental palladium levels are increasing because of anthropogenic activities. The considerable mobility of the metal, due to solubilisation phenomena, and its known bioavailability may indicate interactions with higher organisms. The aim of the study was to determine the Pd uptake and distribution in the various organs of the higher plant Pisum sativum and the metal-induced effects on its growth and reproduction. P. sativum was grown in vermiculite with a modified Hoagland's solution of nutrients in the presence of Pd at concentrations ranging 0.10-25 mg/L. After 8-10 weeks in a controlled environment room, plants were harvested and dissected to isolate the roots, stems, leaves, pods and peas. The samples were analysed for Pd content using AAS and SEM-EDX. P. sativum absorbed Pd, supplied as K₂PdCl₄, beginning at seed germination and continuing throughout its life. Minimal doses (0.10-1.0 mg Pd/L) severely inhibited pea reproductive processes while showing a peculiar hormetic effect on root development. Pd concentrations ≥1 mg/L induced developmental delay, with late growth resumption, increased leaf biomass (up to 25%) and a 15-20% reduction of root mass. Unsuccessful repeated blossoming efforts led to misshapen pods and no seed production. Photosynthesis was also disrupted. The absorbed Pd (ca. 0.5 % of the supplied metal) was primarily fixed in the root, specifically in the cortex, reaching concentrations up to 200 μg/g. The metal moved through the stem (up to 1 μg/g) to the leaves (2 μg/g) and pods (0.3 μg/g). The presence of Pd in the pea fruits, together with established evidence of environmental Pd accumulation and bioavailability, suggests possible contamination of food plants and propagation in the food chain and must be the cause for concern.
authors: Dipartimento di Scienze Teoriche e Applicate, Università degli Studi dell'Insubria, via J. H. Dunant 3, 21100, Varese, Italy.
authors: Matteo
authors: M
authors: Ronchini
authors: Laura
authors: L
authors: Cherchi
authors: Simone
authors: S
authors: Cantamessa
authors: Marco
authors: M
authors: Lanfranchi
authors: Alberto
authors: A
authors: Vianelli
authors: Paolo
authors: P
authors: Gerola
authors: Graziella
authors: G
authors: Berta
authors: Alessandro
authors: A
authors: Fumagalli
mesh_terms: D018533:Biomass; D002712:Chlorides / Q000737:chemistry / Q000378:metabolism*; D010165:Palladium / Q000737:chemistry / Q000378:metabolism*; D018532:Pisum sativum / Q000187:drug effects* / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects*; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D012098:Reproduction; D012639:Seeds / Q000254:growth & development
chemical_list: D002712:Chlorides; C117870:potassium palladium chloride; D010165:Palladium
references: Science. 1983 Nov 11;222(4624):621-3
references: 17843840
references: Aquat Toxicol. 2011 Mar;102(1-2):104-13
references: 21371618
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:601-639
references: 15012221
references: Electrophoresis. 2004 Jun;25(12):1758-64
references: 15213973
references: Environ Toxicol Chem. 2012 Jun;31(6):1231-8
references: 22505271
references: Environ Sci Technol. 2007 Jan 15;41(2):451-6
references: 17310706
references: Chemosphere. 2008 Dec;74(1):6-12
references: 18992910
references: J Biomed Sci. 1997;4(5):256-259
references: 12386388
references: Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:561-591
references: 11337409
references: J Hyg Epidemiol Microbiol Immunol. 1984;28(1):17-24
references: 6715873
references: Plant Sci. 2013 Feb;199-200:79-90
references: 23265321
references: Annu Rev Plant Biol. 2007;58:459-81
references: 17288534
references: Environ Sci Pollut Res Int. 2010 Mar;17(3):665-73
references: 19449049
references: Plant Physiol. 1994 Feb;104(2):649-655
references: 12232115
references: Annu Rev Plant Biol. 2008;59:89-113
references: 18444897
references: Biochim Biophys Acta. 2009 Oct;1787(10):1151-60
references: 19463778
references: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2006;41(3):397-414
references: 16484072
references: Sci Total Environ. 2004 Dec 1;334-335:21-38
references: 15504490
references: Plant Physiol. 2004 Apr;134(4):1793-802
references: 15064381
references: Environ Monit Assess. 2012 Dec;184(12):7373-82
references: 22322405
references: Environ Sci Technol. 2001 Mar 15;35(6):1031-6
references: 11347910
references: Biochim Biophys Acta. 2012 Jan;1817(1):209-17
references: 21565163
references: Environ Pollut. 2008 May;153(2):323-32
references: 18191314
references: Plant Physiol. 1987 Nov;85(3):801-3
references: 16665780
references: Free Radic Biol Med. 1997;23(1):155-61
references: 9165308
references: Plant Biol (Stuttg). 2008 Mar;10(2):272-6
references: 18304202
references: Sci Total Environ. 2004 Jan 5;318(1-3):1-43
references: 14654273
references: Environ Pollut. 2008 Nov;156(2):341-7
references: 18355951
references: Environ Pollut. 2010 Mar;158(3):873-82
references: 19815321
references: Chemosphere. 2004 Jun;55(9):1159-68
references: 15081756
references: Chemosphere. 2010 Sep;80(11):1247-54
references: 20655570
references: Plant Physiol. 1984 Nov;76(3):691-3
references: 16663907
references: Environ Pollut. 2010 Jan;158(1):325-38
references: 19647914
references: Ecotoxicol Environ Saf. 2008 Sep;71(1):76-85
references: 18082263
references: Free Radic Biol Med. 2004 Jun 1;36(11):1408-17
references: 15135177
references: Ecotoxicol Environ Saf. 2001 Jan;48(1):51-5
references: 11161677
references: Ecotoxicology. 2013 Apr;22(3):476-85
references: 23359063
references: Sci Total Environ. 2004 Apr 25;322(1-3):95-108
references: 15081741