title: Effects of ERK1/2/PPARα/SCAD signal pathways on cardiomyocyte hypertrophy induced by insulin-like growth factor 1 and phenylephrine.
abstract: OBJECTIVE
  Short-chain acyl-CoA dehydrogenase (SCAD) is a key enzyme in fatty acid oxidation. In the present study we aim to investigate the changes in SCAD between pathological and physiological cardiomyocyte hypertrophy. We also explore the different signaling pathways of pathological and physiological cardiomyocyte hypertrophy.
  
  
  METHODS
  After neonatal rat cardiomyocytes were treated as setups, cell surface area, expression of SCAD, PPARα, phospho-ERK1/2, activity of SCAD, free fatty acid content and ATP content in the cardiomyocytes were measured.
  
  
  RESULTS
  Neonatal rat cardiomyocytes treated by PE showed an increased cell surface area and free fatty acid content, increased ERK1/2 phosphorylation, decreased expression of PPARα, decreased expression and activity of SCAD and decreased levels of ATP. Neonatal rat cardiomyocytes treated by IGF-1 showed the reverse effects except for the cell surface area. PPARα inhibitor GW6471 and PPARα activator Fenofibrate treatments abrogated the effects induced by IGF-1 and PE in cardiomyocytes respectively, as well as ERK1/2 activator EGF and ERK1/2 inhibitor PD98059.
  
  
  CONCLUSIONS
  SCAD has different changes between pathological and physiological cardiomyocyte hypertrophy. The ERK1/2/PPARα/SCAD signaling pathways play different roles in pathological and physiological cardiomyocyte hypertrophy. SCAD may be used as a new target to prevent the development of pathological cardiac hypertrophy.
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Qiuju
authors: Q
authors: Huang
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Jinxian
authors: J
authors: Huang
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Zhenhua
authors: Z
authors: Zeng
authors: Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Jiani
authors: J
authors: Luo
authors: Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Peiqing
authors: P
authors: Liu
authors: Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Shaorui
authors: S
authors: Chen
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Bing
authors: B
authors: Liu
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Xuediao
authors: X
authors: Pan
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China.
authors: Linquan
authors: L
authors: Zang
authors: Department of Clinical Pharmacy, GuangDong Pharmaceutical University, Guangzhou 510006, China. Electronic address: zhousg201014@163.com.
authors: Sigui
authors: S
authors: Zhou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D044926:Butyryl-CoA Dehydrogenase / Q000378:metabolism*; D006332:Cardiomegaly / Q000188:drug therapy / Q000473:pathology*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism*; D011345:Fenofibrate / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000502:physiology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology*; D010080:Oxazoles / Q000494:pharmacology; D047493:PPAR alpha / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D005419:Flavonoids; C449302:GW 6471; D010080:Oxazoles; D047493:PPAR alpha; D010656:Phenylephrine; D014443:Tyrosine; D007334:Insulin-Like Growth Factor I; D044926:Butyryl-CoA Dehydrogenase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D011345:Fenofibrate
keywords: Cardiomyocyte hypertrophy; Extracellular signal-regulated kinase1/2; Fatty acid oxidation; Peroxisome proliferator activated receptor α; Short-chain acyl-CoA dehydrogenase