title: Self-assembled lysozyme/carboxymethylcellulose nanogels for delivery of methotrexate.
abstract: Nanogels (NGs) were fabricated with lysozyme and carboxymethylcellulose via a green self-assembly method. The prepared NGs were characterized by dynamic light scattering (DLS), zeta potential, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Pyrene and isothiocyanate were introduced as fluorescent probes to research the hydrophobic area of the NGs and cells endocytosis, respectively. Methotrexate (MTX) was used to investigate the drug encapsulation property of the NGs. It turned out to be that the drug loaded NGs were regular spherical shape with a hydrodynamic diameter of about 123 nm. The drug loading efficiency was about 14.2%. The NGs can slowly release the drug and increase the bioavailability of the loaded drug. The NGs are promising carriers for the delivery of drugs and other bioactive molecules.
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science, Yangtze University, Jingzhou 434025, China; Jingchu Food Research and Development Center, Yangtze University, Jingzhou 434025, China.
authors: Zhenshun
authors: Z
authors: Li
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Wei
authors: W
authors: Xu
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Chunlan
authors: C
authors: Zhang
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China.
authors: Yijie
authors: Y
authors: Chen
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. Electronic address: libinfood@mail.hzau.edu.cn.
authors: Bin
authors: B
authors: Li
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D065546:Drug Liberation; D000067493:Dynamic Light Scattering; D004705:Endocytosis / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D061986:MCF-7 Cells; D008727:Methotrexate / Q000494:pharmacology*; D009113:Muramidase / Q000737:chemistry*; D000080385:Nanogels; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate; D002266:Carboxymethylcellulose Sodium; D008727:Methotrexate
keywords: Drug carriers; Nanogels; Self-assembly