title: Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.
abstract: Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Jinghui
authors: J
authors: Zheng
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Jian
authors: J
authors: Liang
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Xin
authors: X
authors: Deng
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Xiaofeng
authors: X
authors: Chen
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Fasheng
authors: F
authors: Wu
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Xiaofang
authors: X
authors: Zhao
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Yuan
authors: Y
authors: Luo
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Lei
authors: L
authors: Fu
authors: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
authors: Zuling
authors: Z
authors: Jiang
keywords: Buyang Huanwu decoction; bone marrow mesenchymal stem cells; cell signal transduction pathway; extracellular signal-regulated protein kinase; mitogen-activated protein kinase signaling pathway; nestin; neural regeneration; neuron specific enolase
references: Anticancer Res. 2005 May-Jun;25(3A):1803-10
references: 16033103
references: Dev Neurosci. 2006;28(1-2):34-48
references: 16508302
references: J Cell Biochem. 2005 Mar 1;94(4):645-55
references: 15547939
references: Hypertension. 2008 Jun;51(6):1537-44
references: 18426993
references: Methods Mol Biol. 2011;698:295-303
references: 21431527
references: Se Pu. 2007 Sep;25(5):723-7
references: 18161326
references: Evid Based Complement Alternat Med. 2011;2011:561396
references: 21792360
references: J Ethnopharmacol. 2011 Sep 1;137(1):214-20
references: 21605653
references: J Biochem. 2010 Sep;148(3):299-308
references: 20587647
references: J AOAC Int. 2011 Jan-Feb;94(1):51-70
references: 21391481
references: Biochem Biophys Res Commun. 2008 Aug 8;372(4):520-4
references: 18502199
references: Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 Jan;40(1):27-32
references: 21319370
references: Stem Cells. 2007 Nov;25(11):2739-49
references: 17656645
references: J Ethnopharmacol. 2009 Jul 15;124(2):219-23
references: 19409971
references: Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Jan;41(1):53-6
references: 20369470
references: Cell Signal. 2006 Apr;18(4):519-30
references: 16098715
references: Dev Dyn. 2007 Dec;236(12):3297-308
references: 18000980
references: J Tradit Chin Med. 2010 Dec;30(4):278-82
references: 21287785