title: Formulation and optimization of mitochondria-targeted polymeric nanoparticles.
abstract: Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria-targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer poly(DL-lactide-co-glycolide)-b-polyethylene glycol-TPP and a nontargeted poly(DL-lactide-co-glycolide)-b-polyethylene glycol polymer were synthesized and their NPs were prepared. A nanoprecipitation method combined with polymer blending technology was adopted in order to get suitable size and charged NPs for efficient mitochondrial trafficking.
authors: NanoTherapeutics Research Laboratory, Department of Chemistry, University of Georgia, Athens, GA, 30606, USA.
authors: Sean
authors: S
authors: Marrache
authors: Rakesh K
authors: RK
authors: Pathak
authors: Shanta
authors: S
authors: Dhar
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D009943:Organophosphorus Compounds; D011108:Polymers; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols