title: A rapid SPE-based analytical method for UPLC/MS/MS determination of aminoglycoside antibiotic residues in bovine milk, muscle, and kidney.
abstract: An SPE-based cleanup protocol was developed for ultra-performance LC (UPLC)/MS/MS determination of residues of the common aminoglycoside antibiotics streptomycin, dihydrostreptomycin, neomycin, and gentamicin in bovine milk, kidney, and muscle. Recoveries for all compounds except neomycin ranged from 80 to 104% for all matrixes studied; recoveries for neomycin ranged from 71 to 84%. Intraday and interday precision data were under 15% for all sample matrixes. Compared with other recently reported cleanup methods, less sample is required, the use of potentially dangerous reagents is minimized, and fewer manipulations are required by the analyst. A high throughput 96-well plate format was used for SPE cleanup and UPLC/MS analysis.
authors: Michael S
authors: MS
authors: Young
authors: Kim
authors: K
authors: van Tran
authors: Evelyn
authors: E
authors: Goh
authors: Jeremy C
authors: JC
authors: Shia
mesh_terms: D000617:Aminoglycosides / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D007668:Kidney / Q000737:chemistry; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents