title: Cytotoxicity of aqueous extracts of Rosmarinus officinalis L. (Labiatae) in plant test system.
abstract: This study investigated the cytotoxic activity of Rosmarinus officinalis L. (rosemary) aqueous extract on the cell cycle of Allium cepa. To this end, crude aqueous leaf extracts at four concentrations, 0.02, 0.04, 0.06 and 0.08 mg/mL, were tested on A. cepa meristematic root cells, at exposure times of 24 and 48 h. Slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5,000 for each control group and concentration. The four concentrations tested, including the lowest and considered ideal for use, at all exposure times, showed a significant antiproliferative effect on the cell cycle of this test system and presented a high number of cells in prophase. Our results evidenced the cytotoxicity of rosemary extracts, under the studied conditions.
authors: Núcleo de Pesquisa Aplicada à Saúde e ao Meio-ambiente - NUPBSAM, Laboratório de Citogenética Vegetal e Animal, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros - CSHNB, Picos, PI, Brazil.
authors: G H S
authors: GH
authors: Cardoso
authors: Núcleo de Pesquisa Aplicada à Saúde e ao Meio-ambiente - NUPBSAM, Laboratório de Citogenética Vegetal e Animal, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros - CSHNB, Picos, PI, Brazil.
authors: E B S
authors: EB
authors: Dantas
authors: Núcleo de Pesquisa Aplicada à Saúde e ao Meio-ambiente - NUPBSAM, Laboratório de Citogenética Vegetal e Animal, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros - CSHNB, Picos, PI, Brazil.
authors: F R C
authors: FR
authors: Sousa
authors: Núcleo de Pesquisa Aplicada à Saúde e ao Meio-ambiente - NUPBSAM, Laboratório de Citogenética Vegetal e Animal, Universidade Federal do Piauí - UFPI, Campus Senador Helvídio Nunes de Barros - CSHNB, Picos, PI, Brazil.
authors: A P
authors: AP
authors: Peron
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D019697:Onions / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000633:toxicity*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects*; D027542:Rosmarinus / Q000633:toxicity*; D013997:Time Factors
chemical_list: D010936:Plant Extracts