title: Reactive oxygen species scavenging activity of Jixueteng evaluated by electron spin resonance (ESR) and photon emission.
abstract: Jixueteng, the dried stem of Spatholobus suberectus Dunn (Leguminosae), is a traditional Chinese herbal medicine that is commonly classified as a herb that promotes blood circulation and can be used to treat blood stasis. The aim of this study was to examine the reactive oxygen species (ROS) scavenging activity of Jixueteng and other herbal medicines. The ROS scavenging activities of the water extracts of Jixueteng, Cnidium officinale and Salvia miltiorrhiza were examined using an electron spin resonance (ESR) technique and faint luminescence measurement. The ESR signal intensities of the superoxide anion (O2·) and hydroxyl radical (HO·) were reduced more by Jixueteng than the other herbal medicines we tested. High photon emission intensity to hydrogen peroxide (H202) and HO· was observed in Jixueteng using the XYZ chemiluminescence system that was used as faint luminescence measurement and analysis. The results of the present study revealed that the ROS scavenging activity of 8% Jixueteng was the strongest among the herbal medicines we tested. It has been reported that Jixueteng includes various polyphenols. In the ROS scavenging activity by Jixueteng, it is supposed that the antioxidant activity caused by these polyphenols would contribute greatly. In conclusion, a water extract component of Jixueteng had potent free radical scavenging activity and an antioxidative effect that inhibited the oxidative actions of O2·⁻, H2O2 and HO·. Therefore, Jixueteng represents a promising therapeutic drug for reactive oxygen-associated pathologies.
authors: Toshizo
authors: T
authors: Toyama
authors: Satoko
authors: S
authors: Wada-Takahashi
authors: Maomi
authors: M
authors: Takamichi
authors: Kiyoko
authors: K
authors: Watanabe
authors: Ayaka
authors: A
authors: Yoshida
authors: Fumihiko
authors: F
authors: Yoshino
authors: Chihiro
authors: C
authors: Miyamoto
authors: Yojiro
authors: Y
authors: Maehata
authors: Shuta
authors: S
authors: Sugiyama
authors: Shun-Suke
authors: SS
authors: Takahashi
authors: Kazuo
authors: K
authors: Todoki
authors: Masaichi-Chang-Il
authors: MC
authors: Lee
authors: Nobushiro
authors: N
authors: Hamada
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008163:Luminescent Measurements; D017785:Photons; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species