title: [Total saponins of Clematis inhibits JAK2/STAT3 signal pathway of adjuvant-induced arthritis rats].
abstract: OBJECTIVE
  To evaluate the effect of total saponins of Clematis (TSC) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signal transduction pathway in adjuvant-induced arthritis (AIA) rats and investigate the probable mechanisms.
  
  
  METHODS
  Sixty male SD rats were randomized divided into six groups: normal group, model control group, (50, 100, 200 mg/kg) TSC group, and tripterygium glycosides tablet (10 mg/kg) group (10 rats in each group). Except the normal group, AA models were induced with Freund's complete adjuvant. Twelve days after modeling, corresponding drugs were given to rats by intragastric administration, q.d, for 16 days. Then, the effects of drugs on the body mass and paw swelling of AA rats were observed, and ankle-joint samples were taken to examine the degree of AA by HE staining. Moreover, real-time fluorescent quantitative PCR was performed to detect the expressions of JAK2 and STAT3 mRNA, and Western blotting to determine the expression of p-JAK2, JAK2, p-STAT3, and STAT3 protein.
  
  
  RESULTS
  Compared with the model group, TSC not only effectively alleviated the body mass loss and significantly inhibited paw swelling in AA rats, but also significantly inhibited inflammatory cell infiltration, pannus formation and tissue proliferation. Furthermore, TSC treatment obviously decreased mRNA expressions of JAK2 and STAT3 as well as the relative expressions of p-JAK2/JAK2 protein and p-STAT3/STAT3 protein in synovial tissues.
  
  
  CONCLUSIONS
  TSC can inhibit JAK2/STAT3 signal pathway by decreasing the expressions of JAK2 and STAT3 mRNA and the relative expressions of p-JAK2/JAK2 protein and p-STAT3/STAT3 protein of synovial tissues in AA rats.
authors: Department of Graduate, Anhui University of Chinese Medicine, Hefei 230038, China.
authors: Longfei
authors: L
authors: Deng
authors: First Affiliated Hospital, Anhui University of Chinese Medicine, State Administration of Traditional Chinese Medicine Level-3 Laboratory for Traditional Chinese Medicine Preparation, Hefei 230031, China.
authors: Yongzhong
authors: Y
authors: Wang
authors: First Affiliated Hospital, Anhui University of Chinese Medicine, State Administration of Traditional Chinese Medicine Level-3 Laboratory for Traditional Chinese Medicine Preparation, Hefei 230031, China.
authors: Yanquan
authors: Y
authors: Han
authors: First Affiliated Hospital, Anhui University of Chinese Medicine, State Administration of Traditional Chinese Medicine Level-3 Laboratory for Traditional Chinese Medicine Preparation, Hefei 230031, China.
authors: Hui
authors: H
authors: Jiang
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D039324:Clematis / Q000737:chemistry*; D015536:Down-Regulation; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D012503:Saponins / Q000008:administration & dosage*; D015398:Signal Transduction; D013583:Synovial Membrane / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D050796:STAT3 Transcription Factor; D012503:Saponins; C507927:Jak2 protein, rat; D053614:Janus Kinase 2