title: Probing peptide and protein insertion in a biomimetic S-layer supported lipid membrane platform.
abstract: The most important aspect of synthetic lipid membrane architectures is their ability to study functional membrane-active peptides and membrane proteins in an environment close to nature. Here, we report on the generation and performance of a biomimetic platform, the S-layer supported lipid membrane (SsLM), to investigate the structural and electrical characteristics of the membrane-active peptide gramicidin and the transmembrane protein α-hemolysin in real-time using a quartz crystal microbalance with dissipation monitoring in combination with electrochemical impedance spectroscopy. A shift in membrane resistance is caused by the interaction of α-hemolysin and gramicidin with SsLMs, even if only an attachment onto, or functional channels through the lipid membrane, respectively, are formed. Moreover, the obtained results did not indicate the formation of functional α-hemolysin pores, but evidence for functional incorporation of gramicidin into this biomimetic architecture is provided.
authors: Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria. sdamiati@kau.edu.sa.
authors: Samar
authors: S
authors: Damiati
authors: Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria. angelika.schrems@gmail.com.
authors: Angelika
authors: A
authors: Schrems
authors: Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria. eva.sinner@boku.ac.at.
authors: Eva-Kathrin
authors: EK
authors: Sinner
authors: Institute for Biophysics, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria. uwe.sleytr@boku.ac.at.
authors: Uwe B
authors: UB
authors: Sleytr
authors: Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria. bernhard.schuster@boku.ac.at.
authors: Bernhard
authors: B
authors: Schuster
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D032701:Biomimetics; D058266:Dielectric Spectroscopy; D006096:Gramicidin / Q000737:chemistry / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D058525:Quartz Crystal Microbalance Techniques; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D009004:Monosaccharide Transport Proteins; D010455:Peptides; C120455:SbpA protein, bacteria; D053835:Unilamellar Liposomes; D006096:Gramicidin
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