title: Liver, biliary and pancreatic injuries in pancreaticobiliary maljunction model in cats.
abstract: BACKGROUND
  Pancreaticobiliary maljunction is a high risk factor of pancreatitis and biliary tract cancer. How this maljunction affects the liver remains obscure. This study aimed to examine the effects of pancreaticobiliary maljunction on the liver, pancreas and gallbladder in a cat model.
  
  
  METHODS
  A model of choledocho-pancreatic side-to-side ductal anastomosis was created in ten cats. Before the procedure, a small piece of tissue from the liver, pancreas and gallbladder was collected as a control. The common channel formation was checked by cholecystography. The livers, pancreases and gallbladders of these cats were harvested for histological examination. The expression of proliferating cell nuclear antigen in the gallbladder was examined with immunohistochemistry.
  
  
  RESULTS
  Seven of the 10 cats survived for 6 months after surgery. The color of the liver was darker in the PBM model than the control specimen, with nodules on the surface. Histological examination showed ballooning changes and inflammatory infiltrations and the histopathological score increased significantly (P<0.05). Also, mitochondria swelling and lipid droplet in cytoplasm were observed under an electron microscope. The pancreas also appeared darker in the PBM model than the control specimen and dilated pancreatic ducts were found in three cats. Histopathological examination revealed vascular proliferation and inflammatory infiltration with numerous neutrophils. Gallbladder epithelial cells were featured by expanded endoplasmic reticulum, increased intercellular space and cellular nucleus deformation. The positive cells of proliferating cell nuclear antigen were increased significantly (P<0.05).
  
  
  CONCLUSIONS
  The present study demonstrated that pancreaticobiliary maljunction can lead to the injuries of the liver, pancreas and gallbladder.
authors: Division of General Surgery, Soochow University Affiliated Children's Hospital, Suzhou 215003, China. wj196312@vip.163.com.
authors: Feng
authors: F
authors: Chen
authors: Lin
authors: L
authors: Tang
authors: Zhi-Qi
authors: ZQ
authors: Zhang
authors: Bing-Wei
authors: BW
authors: Jin
authors: Wei-Feng
authors: WF
authors: Dong
authors: Jian
authors: J
authors: Wang
authors: Shun-Gen
authors: SG
authors: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002415:Cats; D049109:Cell Proliferation; D004065:Digestive System Abnormalities / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D005704:Gallbladder / Q000378:metabolism / Q000473:pathology* / Q000601:surgery / Q000648:ultrastructure; D008099:Liver / Q000473:pathology* / Q000601:surgery / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000473:pathology; D008933:Mitochondrial Swelling; D020556:Neutrophil Infiltration; D010179:Pancreas / Q000473:pathology* / Q000601:surgery / Q000648:ultrastructure; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen