title: Isolation of Sutherlandioside B fromSutherlandia frutescensby Spiral Countercurrent Chromatography.
abstract: The introduction of spiral countercurrent chromatography in the last few years using new separation columns such as the spiral tubing support rotor has enabled the application of more polar volatile solvent systems for natural products separation. This method can be applied to water soluble compounds and their metabolites. We have used spiral countercurrent chromatography with the spiral tubing support rotor to fractionaten-butanol extracts of an African plantSutherlandia frutescensand have determined conditions by which the predominant cycloartane glycoside (sutherlandioside B) can be purified in good yield. A solvent system of ethyl acetate, methanol, and water was modified by adding n-butanol to separate sutherlandioside B from other compounds. With the optimal amount of n-butanol in the two-phase solvent system with the lower aqueous phase mobile, the target compound was eluted well separated from the other components. The purity of sutherlandioside B was determined by high performance liquid chromatography/mass spectrometry analysis and the yield compares favorably with the content in bulk material.
authors: Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA.
authors: Korey J
authors: KJ
authors: Brownstein
authors: Veterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, MO 65211, USA.
authors: George E
authors: GE
authors: Rottinghaus
authors: CC Biotech LLC., 9700 Great Seneca Highway, Rockville, MD 20850, USA.
authors: Martha
authors: M
authors: Knight
authors: Laboratory of Bioseparations Technology, Center of Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Yoichiro
authors: Y
authors: Ito
authors: Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA.
authors: William
authors: W
authors: Folk
keywords: Sutherlandia frutescens; cycloartane glycoside; n-butanol-aqueous solvent systems; spiral countercurrent chromatography; spiral tubing support rotor; sutherlandioside
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