title: Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae.
abstract: Many pathovars of plant pathogenic bacteria Xanthomonas species inject transcription activator-like (TAL) effectors into plant host cells to promote disease susceptibility or trigger disease resistance. The rice TAL effector-dependent disease resistance gene Xa10 confers narrow-spectrum race-specific resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight disease in rice. To generate broad-spectrum and durable resistance to Xoo, we developed a modified Xa10 gene, designated as Xa10(E5) . Xa10(E5) has an EBE-amended promoter containing 5 tandemly arranged EBEs each responding specifically to a corresponding virulent or avirulent TAL effector and a stable transgenic rice line containing Xa10(E5) was generated in the cultivar Nipponbare. The Xa10(E5) gene was specifically induced by Xoo strains that harbour the corresponding TAL effectors and conferred TAL effector-dependent resistance to the pathogens at all developmental stages of rice. Further disease evaluation demonstrated that the Xa10(E5) gene in either Nipponbare or 9311 genetic backgrounds provided broad-spectrum disease resistance to 27 of the 28 Xoo strains collected from 11 countries. The development of Xa10(E5) and transgenic rice lines provides new genetic materials for molecular breeding of rice for broad-spectrum and durable disease resistance to bacterial blight.
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.|Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
authors: Xuan
authors: X
authors: Zeng
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors: Dongsheng
authors: D
authors: Tian
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors: Keyu
authors: K
authors: Gu
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors: Zhiyun
authors: Z
authors: Zhou
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors: Xiaobei
authors: X
authors: Yang
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.|Rice Research Institute, Anhui Academy of Agricultural Sciences, Anhui, China.
authors: Yanchang
authors: Y
authors: Luo
authors: Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
authors: Frank F
authors: FF
authors: White
authors: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.|Department of Biological Sciences, National University of Singapore, Singapore, Singapore.|School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors: Zhongchao
authors: Z
authors: Yin
mesh_terms: D060467:Disease Resistance / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D010940:Plant Proteins
keywords: R gene; Rice; TAL effector; Xa10; Xanthomonas oryzae pv. oryzae; bacterial blight