title: Role of PDGFR-β/PI3K/AKT signaling pathway in PDGF-BB induced myocardial fibrosis in rats.
abstract: OBJECTIVE
  To investigate the role of PDGFR-β/PI3K/AKT signaling pathway in the myocardial fibrosis.
  
  
  METHODS
  CFs were divided into following 4 groups: control group (CON), PDGF-BB group (P), PDGF-BB+IMA group (IMA), and PDGF-BB+LY294002 (LY).
  
  
  RESULTS
  Immunofluorescence staining showed about 90% of cells were positive for vimentin and 10% for α-SMA. After incubation for 7 days, fluorescence microscopy revealed more than 90% of cells were positive for α-SMA, which was significantly higher than that in CON group (P < 0.01), but markedly lower than that in IMA group and LY group (P < 0.01). The mRNA and protein expression of PDGFR-β, Col I, Col III, PI3K and Akt increased dramatically at 48 h after PDGF-BB treatment when compared with CON group (P < 0.01). However, IMA and LY294002 significantly inhibited the expression of PDGFR-β and p-PI3K (P < 0.05). In addition, the mRNA expression of PDGFR-β, PI3K and Akt in IMA group and LY group was also markedly lower than those in P group (P < 0.01), and the mRNA and protein expression of Col I and Col III reduced remarkably when compared with P group (P < 0.01). Of note, the mRNA expression of PDGFR-α was comparable among 4 groups, and PDGFR-β expression after PDGF-BB treatment increased significantly when compared with PDGFR-α expression (P < 0.01).
  
  
  CONCLUSIONS
  PDGF-BB may induce CF proliferation and their transformation into myofibroblasts, which leads to increased synthesis of collagen, resulting in myocardial fibrosis. This is closely associated with PDGFR-β, but not PDGFR-α. PDGFR-β/PI3K/Akt signaling pathway is involved in the PDGF-BB induced myocardial fibrosis.
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Hai
authors: H
authors: Fan
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Likun
authors: L
authors: Ma
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Bin
authors: B
authors: Fan
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Jiawei
authors: J
authors: Wu
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Zhe
authors: Z
authors: Yang
authors: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei 230001, China.
authors: Lei
authors: L
authors: Wang
keywords: Myocardial fibrosis; myofibroblasts; platelet-derived growth factor; signaling pathway
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