title: Integrated production of polyhydroxyalkanoates (PHAs) with municipal wastewater and sludge treatment at pilot scale.
abstract: A pilot-scale process was operated over 22 months at the Brussels North Wastewater Treatment Plant (WWTP) in order to evaluate polyhydroxyalkanoate (PHA) production integration with services of municipal wastewater and sludge management. Activated sludge was produced with PHA accumulation potential (PAP) by applying feast-famine selection while treating the readily biodegradable COD from influent wastewater (average removals of 70% COD, 60% CODsol, 24% nitrogen, and 46% phosphorus). The biomass PAP was evaluated to be in excess of 0.4gPHA/gVSS. Batch fermentation of full-scale WWTP sludge at selected temperatures (35, 42 and 55 °C) produced centrate (6-9.4 gCODVFA/L) of consistent VFA composition, with optimal fermentation performance at 42 °C. Centrate was used to accumulate PHA up to 0.39 gPHA/gVSS. The centrate nutrients are a challenge to the accumulation process but producing a biomass with 0.5 gPHA/gVSS is considered to be realistically achievable within the typically available carbon flows at municipal waste management facilities.
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden. Electronic address: fernando.morgan@anoxkaldnes.com.
authors: F
authors: F
authors: Morgan-Sagastume
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: M
authors: M
authors: Hjort
authors: Dept. of Biotechnology and Biosystems, Veolia Recherche et Innovation (VERI) - Centre de Recherche de Maisons Laffitte, Chemin de la Digue - BP 76, 78603 Maisons-Laffitte, France.
authors: D
authors: D
authors: Cirne
authors: Dept. of Biotechnology and Biosystems, Veolia Recherche et Innovation (VERI) - Centre de Recherche de Maisons Laffitte, Chemin de la Digue - BP 76, 78603 Maisons-Laffitte, France.
authors: F
authors: F
authors: Gérardin
authors: Dept. of Biotechnology and Biosystems, Veolia Recherche et Innovation (VERI) - Centre de Recherche de Maisons Laffitte, Chemin de la Digue - BP 76, 78603 Maisons-Laffitte, France.
authors: S
authors: S
authors: Lacroix
authors: Dept. of Biotechnology and Biosystems, Veolia Recherche et Innovation (VERI) - Centre de Recherche de Maisons Laffitte, Chemin de la Digue - BP 76, 78603 Maisons-Laffitte, France.
authors: G
authors: G
authors: Gaval
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: L
authors: L
authors: Karabegovic
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: T
authors: T
authors: Alexandersson
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: P
authors: P
authors: Johansson
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: A
authors: A
authors: Karlsson
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: S
authors: S
authors: Bengtsson
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: M V
authors: MV
authors: Arcos-Hernández
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: P
authors: P
authors: Magnusson
authors: AnoxKaldnes, Veolia Water Technologies, Klosterängsvägen 11A, 226 47 Lund, Sweden.
authors: A
authors: A
authors: Werker
mesh_terms: D061249:Batch Cell Culture Techniques; D001530:Belgium; D018533:Biomass; D002947:Cities; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D009584:Nitrogen / Q000494:pharmacology; D010758:Phosphorus / Q000494:pharmacology; D010865:Pilot Projects; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012722:Sewage; D062065:Wastewater; D018508:Water Purification / Q000379:methods*
chemical_list: D005232:Fatty Acids, Volatile; D009584:Nitrogen; D010758:Phosphorus; D054813:Polyhydroxyalkanoates; D012722:Sewage; D062065:Wastewater
keywords: Municipal wastewater treatment; Pilot scale; Polyhydroxyalkanoate (PHA) production; Sludge fermentation; Volatile fatty acid (VFA)