title: The effects of dimethyl sulfoxide and ethylene glycol as vitrification protectants on different cleavage stages of mouse embryo quality.
abstract: The effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. In this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. Also, vitrification and thawing procedures were the same for all experimental groups. Mouse different embryonic cleavage stages were vitrified in ethylene glycol (EG) plus dimethyl sulfoxide (DMSO) and sucrose (VS-1) and EG plus DMSO (VS-2) and thawed by directly placing the vitrified drop into sucrose solution (TS) at 37 ˚C. High recovery (72-97%) of morphologically normal embryos was evident following vitrification and thawing. Development of the vitrified morulae into blastocysts (92%) was higher (p < 0.05). The amount of zygote and 2-cell stages that achieved to blastocyst stage was very low. With progressing the embryo cleavage to morula stage, the embryos that reached to blastocyst were increased to its maximum number. We concluded that the modified vitrification procedure supported better survival of morula stage compared to other cleavage stages in mouse embryos.
authors: Department of Biology, Faculty of Science, Urmia University, Urmia, Iran;
authors: Mina
authors: M
authors: Ghorbani
authors: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors: Rajabali
authors: R
authors: Sadrkhanlou
authors: Department of Biology, Faculty of Science, Urmia University, Urmia, Iran;
authors: Vahid
authors: V
authors: Nejati
authors: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors: Abbas
authors: A
authors: Ahmadi
authors: Dr. Tizroo Day Care and IVF center, Urmia, Iran.
authors: Gholamreza
authors: G
authors: Tizroo
keywords: Blastocyst; Dimethyl sulfoxide; Ethylene glycol; Morula; Vitrification
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