title: Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development.
abstract: Chemical disinfection of oral biofilms often leaves biofilm structures intact. This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. It was concluded that disinfected biofilm structures favored secondary bacterial adhesion.
authors: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Tatsuya
authors: T
authors: Ohsumi
authors: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Shoji
authors: S
authors: Takenaka
authors: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Rika
authors: R
authors: Wakamatsu
authors: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Yuuki
authors: Y
authors: Sakaue
authors: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
authors: Naoki
authors: N
authors: Narisawa
authors: Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Hidenobu
authors: H
authors: Senpuku
authors: Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Hayato
authors: H
authors: Ohshima
authors: Division of Microbiology and Infectious Diseases, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Yutaka
authors: Y
authors: Terao
authors: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Takashi
authors: T
authors: Okiji
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D003804:Dentin / Q000382:microbiology; D004203:Disinfection; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties
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