title: Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens.
abstract: Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues.
authors: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska , 143 Food Industry Complex, Lincoln, Nebraska 68583-0919, United States.
authors: Shyamali
authors: S
authors: Jayasena
authors: Mieke
authors: M
authors: Smits
authors: Daniëlle
authors: D
authors: Fiechter
authors: Aard
authors: A
authors: de Jong
authors: Julie
authors: J
authors: Nordlee
authors: Joe
authors: J
authors: Baumert
authors: Steve L
authors: SL
authors: Taylor
authors: Raymond H
authors: RH
authors: Pieters
authors: Stef J
authors: SJ
authors: Koppelman
mesh_terms: D055516:2S Albumins, Plant / Q000032:analysis; D000485:Allergens / Q000032:analysis*; D052179:Antigens, Plant / Q000032:analysis; D010367:Arachis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data*; D005188:False Negative Reactions; D006023:Glycoproteins / Q000032:analysis; D008565:Membrane Proteins; D010940:Plant Proteins / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C500890:Ara h 2 allergen, Arachis hypogaea; C549941:Ara h 3 allergen, Arachis hypogea; C492737:Ara h 6 allergen, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins
keywords: Ara h 1; Ara h 2; Ara h 3; Ara h 6; ELISA; allergens; detection; immunoassay; peanut