title: Cytoplasmic transfer methods for studying the segregation of mitochondrial DNA in mice.
abstract: Heteroplasmic mice represent a valuable tool to study the segregation of different mtDNA haplotypes (mtDNAs with differing alleles) in vivo against a defined nuclear background. We describe two methods for the creation of such models, differing in the resulting initial heteroplasmy levels: (1) transfer of ooplasm and (2) fusion of two blastomeres. These methods result in typical heteroplasmy of 5 % and 50 % donor mtDNA, respectively. The choice of method depends on the aim of the study. By means of breeding, even 100 % donor mtDNA can be reached within few generations.
authors: Biomodels Austria, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria, thomas.kolbe@vetmeduni.ac.at.
authors: Thomas
authors: T
authors: Kolbe
authors: Ralf
authors: R
authors: Steinborn
authors: Joerg P
authors: JP
authors: Burgstaller
mesh_terms: D000818:Animals; D001757:Blastomeres; D002459:Cell Fusion; D003593:Cytoplasm / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004624:Embryo Transfer; D006239:Haplotypes; D051379:Mice; D008845:Microinjections / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial