title: Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues.
abstract: S-Adenosylmethionine (SAM) synthase was engineered for biocatalytic production of SAM and long-chain analogues by rational re-design. Substitution of two conserved isoleucine residues extended the substrate spectrum of the enzyme to artificial S-alkylhomocysteines. The variants proved to be beneficial in preparative synthesis of SAM (and analogues) due to a much reduced product inhibition.
authors: Leibniz-Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, D-06120 Halle, Germany. wessjohann@ipb-halle.de.
authors: M
authors: M
authors: Dippe
authors: W
authors: W
authors: Brandt
authors: H
authors: H
authors: Rost
authors: A
authors: A
authors: Porzel
authors: J
authors: J
authors: Schmidt
authors: L A
authors: LA
authors: Wessjohann
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D014644:Genetic Variation / Q000235:genetics; D006710:Homocysteine / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000737:chemistry; D008716:Methionine Adenosyltransferase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D015202:Protein Engineering
chemical_list: D006710:Homocysteine; D008716:Methionine Adenosyltransferase