title: Assessment of mitochondrial Ca²⁺ uptake.
abstract: Mitochondrial Ca(2+) uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca(2+) signals and elaborating the mechanisms that accomplish mitochondrial Ca(2+) uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca(2+) concentration ([Ca(2+)]mito) in a wide range of applications.
authors: Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010, Graz, Austria.
authors: András T
authors: AT
authors: Deak
authors: Claire
authors: C
authors: Jean-Quartier
authors: Alexander I
authors: AI
authors: Bondarenko
authors: Lukas N
authors: LN
authors: Groschner
authors: Roland
authors: R
authors: Malli
authors: Wolfgang F
authors: WF
authors: Graier
authors: Markus
authors: M
authors: Waldeck-Weiermair
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002460:Cell Line; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D018408:Patch-Clamp Techniques
chemical_list: D002118:Calcium