title: Effects on animal wellbeing and sample quality of 2 techniques for collecting blood from the facial vein of mice.
abstract: When sampling blood from mice, several different techniques can be used, with retroorbital sinus sampling traditionally being the most common. Given the severe tissue trauma caused by retroorbital sampling, alternative methods such as the facial vein route have been developed. The aim of this study was to evaluate 2 techniques for facial vein bleeding in conscious mice to ascertain whether differences in clinical outcomes, practicability of sample collection, and hematologic parameters were apparent. Blood samples were obtained from the facial vein of 40 BALB/c mice by using either a 21-gauge needle or a lancet. Subsequently, the protocol was repeated with isoflurane-anesthetized mice sampled by using the lancet method (n = 20). Behavior immediately after sampling was observed, and sample quantity, sampling time, and time until bleeding ceased were measured. Clinical pathology data and hematoma diameter at necropsy were analyzed also. The mean sample quantity collected (approximately 0.2 mL) was comparable among methods, but sampling was much more rapid when mice were anesthetized by using isoflurane. The only other noteworthy finding was a significantly reduced number of platelets in samples from anesthetized mice. Adverse, ongoing clinical signs were rare regardless of the method used. The results revealed no significant differences in welfare implications or blood sample quality among the methods or between conscious and anesthetized mice. Therefore, any of the methods we evaluated for obtaining blood samples from the facial vein are appropriate for use in research studies.
authors: School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, SA Australia. cassie.francisco@student.adelaide.edu.au.
authors: Cassie C
authors: CC
authors: Francisco
authors: School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, SA Australia.
authors: Gordon S
authors: GS
authors: Howarth
authors: School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, SA Australia.
authors: Alexandra L
authors: AL
authors: Whittaker
mesh_terms: D000827:Animal Welfare; D000818:Animals; D001800:Blood Specimen Collection / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D013047:Specific Pathogen-Free Organisms
references: Lab Anim. 2009 Jul;43(3):255-60
references: 19237457
references: J Am Assoc Lab Anim Sci. 2008 May;47(3):8-15
references: 18459706
references: Lab Anim. 2010 Oct;44(4):352-8
references: 20696790
references: Lab Anim (NY). 2005 Oct;34(9):39-43
references: 16195737
references: Contemp Top Lab Anim Sci. 2005 Mar;44(2):41-5
references: 15773775
references: J Am Assoc Lab Anim Sci. 2007 Jan;46(1):52-7
references: 17203917
references: J Am Assoc Lab Anim Sci. 2010 Jul;49(4):400; author reply 400
references: 20819382
references: Lab Anim. 2011 Apr;45(2):114-20
references: 21382833
references: Lab Anim. 1988 Apr;22(2):177-82
references: 3392954
references: Lab Anim. 2004 Jan;38(1):64-9
references: 14979990
references: Am J Vet Res. 1990 Nov;51(11):1841-6
references: 2240810
references: J Appl Toxicol. 2001 Jan-Feb;21(1):15-23
references: 11180276
references: J Am Assoc Lab Anim Sci. 2010 Mar;49(2):202-6
references: 20353696
references: Am J Physiol Regul Integr Comp Physiol. 2002 Jan;282(1):R77-88
references: 11742826
references: Lab Anim. 1998 Oct;32(4):364-8
references: 9807749
references: Endocrinology. 1970 Oct;87(4):738-43
references: 5453288
references: Lab Anim. 2000 Oct;34(4):351-61
references: 11072855
references: Lab Anim. 1998 Oct;32(4):369-76
references: 9807750
references: Physiol Behav. 1996 Dec;60(6):1565-8
references: 8946506
references: Behav Neurosci. 1987 Feb;101(1):115-9
references: 2950903