title: [Expression of miR-143 and miR-145 and their functional study in gastric carcinoma].
abstract: OBJECTIVE
  To investigate the expressions of miR-143 and miR-145 in gastric cancer tissues and their biological functions.
  
  
  METHODS
  A set of gastric cancer metastasis-related miRNAs was screened by comparing the miRNA profiles between the primary gastric cancer and matched liver metastasis. Among these miRNAs, expressions of miR-143 and miR-145 were further validated. The influence on metastasis was analyzed by transwell assay.
  
  
  RESULTS
  Expressions of miR-143 and miR-145 down-regulated significantly in gastric cancer tissues compared to adjacent normal tissues(miR-143, 0.028±0.005 vs. 0.052±0.014, P=0.058; miR-145, 0.922±0.135 vs. 1.722±0.285, P=0.007), and decreased significantly in the metastatic lesion compared with the primary lesion(miR-143, 0.059±0.025 vs. 0.182±0.045, P=0.021; miR-145, 0.164±0.076 vs. 0.594±0.283, P=0.042). Expressions of miR-143 and miR-145 were significantly correlated(r=0.400, P=0.000), and they synergistically inhibited gastric cancer cell migration.
  
  
  CONCLUSIONS
  miR-143 and miR-145 may take part in the progression of gastric cancer metastasis, and they may have synergetic effects.
authors: Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education), Department of Gastrointestinal Cancer, Beijing Cancer Hospital and Institute, Peking University Cancer Hospital, Peking University School of Oncology, Beijing 100142, China. ligregory369@hotmail.com.
authors: Xiaofang
authors: X
authors: Xing
authors: Ziyu
authors: Z
authors: Li
mesh_terms: D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms; D035683:MicroRNAs; D013274:Stomach Neoplasms
chemical_list: D035683:MicroRNAs