title: Two-photon fluorescent probe for detection of exogenous and endogenous hydrogen persulfide and polysulfide in living organisms.
abstract: Hydrogen persulfide and polysulfide (H2S(n)) are newly discovered intracellular reactive species considered to have high protein S-sulfhydration efficiency. The detection of H2S(n) in living systems is essential for studying their functions but is quite challenging. In this work, we report a two-photon excited fluorescent probe, QS(n), capable of tracking H2S(n) in living organisms. QS(n) exhibited turn-on two-photon fluorescence response upon reaction with H2S(n). With a favorable photophysical property, high specificity, and low cytotoxicity, QS(n) was able to recognize exogenous H2S(n) in living cells. More importantly, it realized for the first time the visualization of endogenous H2S(n) generated in cells overexpressing cystathionine β-synthase and cystathionine γ-lyase, the enzymes responsible for producing endogenous H2S(n). Taking advantage of two-photon microscopy, the probe was also applied to achieve H2S(n) detection in zebrafish embryos and to observe H2S(n) distribution in living organisms.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, and ‡College of Life Science, Wuhan University , Wuhan 430072, China.
authors: Lingyu
authors: L
authors: Zeng
authors: Shiyu
authors: S
authors: Chen
authors: Tian
authors: T
authors: Xia
authors: Wei
authors: W
authors: Hu
authors: Chunya
authors: C
authors: Li
authors: Zhihong
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003541:Cystathionine beta-Synthase / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017785:Photons; D013440:Sulfides / Q000032:analysis*; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D013440:Sulfides; C051552:persulfides; C032915:polysulfide; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide