title: Expanding the potential of chiral chromatography for high-throughput screening of large compound libraries by means of sub-2μm Whelk-O 1 stationary phase in supercritical fluid conditions.
abstract: With the aim of exploring the potential of ultra-fast chiral chromatography for high-throughput analysis, the new sub-2 micron Whelk-O 1 chiral stationary phase (CSP) has been employed in supercritical fluid conditions to screen 129 racemates, mainly of pharmaceutical interest. By using a 5-cm long column (0.46cm internal diameter), a single co-solvent (MeOH) and a 7-min gradient elution, 85% of acidic and neutral analytes considered in this work have been successfully resolved, with resolution (Rs) larger than 2 in more than 65% of cases. Moreover, almost a half of basic samples that, for their own characteristics, are known to be difficult to separate on Whelk-O 1 CSP, have shown Rs greater than 0.3. The screening of the entire library could be accomplished in less than 24h (single run) with 63% of positive score. For well-resolved enantiomers (Rs roughly included between 1 and 3), we show that method transfer from gradient to isocratic conditions is straightforward. In many cases, isocratic ultra-fast separations (with analysis time smaller than 60s) have been achieved by simply employing, as isocratic mobile phase, the eluent composition at which the second enantiomer was eluted in gradient mode. By considering the extension and variety of the library in terms of chemico-physical and structural properties of compounds and numerousness, we believe that this work demonstrates the real potential of the technique for high-throughput enantioselective screening.
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy.
authors: Luca
authors: L
authors: Sciascera
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy.
authors: Omar
authors: O
authors: Ismail
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy. Electronic address: alessia.ciogli@uniroma1.it.
authors: Alessia
authors: A
authors: Ciogli
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy; Chemical and Analytical Development, Novartis Pharma AG, CH-4002, Basel, Switzerland.
authors: Dorina
authors: D
authors: Kotoni
authors: Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Via L. Borsari 46, 44121 Ferrara, Italy.
authors: Alberto
authors: A
authors: Cavazzini
authors: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy.
authors: Lorenzo
authors: L
authors: Botta
authors: Regis Technologies, Inc. , 8210 Austin Avenue, Morton Grove, IL 60053, USA.
authors: Ted
authors: T
authors: Szczerba
authors: Regis Technologies, Inc. , 8210 Austin Avenue, Morton Grove, IL 60053, USA.
authors: Jelena
authors: J
authors: Kocergin
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy.
authors: Claudio
authors: C
authors: Villani
authors: Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, P.le Aldo Moro 5, 00185 Roma, Italy. Electronic address: francesco.gasparrini@uniroma1.it.
authors: Francesco
authors: F
authors: Gasparrini
mesh_terms: D000143:Acids / Q000032:analysis / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation*; D007700:Kinetics; D058115:Lewis Bases / Q000032:analysis / Q000302:isolation & purification; D012545:Schiff Bases / Q000032:analysis / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D000143:Acids; D058115:Lewis Bases; D012545:Schiff Bases
keywords: Enantioselective high throughput screening; Sub-2μm Whelk-O 1; Ultra-fast chiral separations; eUHPSFC