title: Methane production from acid hydrolysates of Agave tequilana bagasse: evaluation of hydrolysis conditions and methane yield.
abstract: Evaluation of diluted acid hydrolysis for sugar extraction from cooked and uncooked Agave tequilana bagasse and feasibility of using the hydrolysates as substrate for methane production, with and without nutrient addition, in anaerobic sequencing batch reactors (AnSBR) were studied. Results showed that the hydrolysis over the cooked bagasse was more effective for sugar extraction at the studied conditions. Total sugars concentration in the cooked and uncooked bagasse hydrolysates were 27.9 g/L and 18.7 g/L, respectively. However, 5-hydroxymethylfurfural was detected in the cooked bagasse hydrolysate, and therefore, the uncooked bagasse hydrolysate was selected as substrate for methane production. Interestingly, results showed that the AnSBR operated without nutrient addition obtained a constant methane production (0.26 L CH4/g COD), whereas the AnSBR operated with nutrient addition presented a gradual methane suppression. Molecular analyses suggested that methane suppression in the experiment with nutrient addition was due to a negative effect over the archaeal/bacterial ratio.
authors: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico.
authors: Jorge
authors: J
authors: Arreola-Vargas
authors: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico.
authors: Valeria
authors: V
authors: Ojeda-Castillo
authors: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico.
authors: Raúl
authors: R
authors: Snell-Castro
authors: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico.
authors: Rosa Isela
authors: RI
authors: Corona-González
authors: División de Ciencias Ambientales. Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Col. Lomas 4(a) Sección, C.P. 78216, San Luis Potosí, SLP, Mexico.
authors: Felipe
authors: F
authors: Alatriste-Mondragón
authors: Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico. Electronic address: hugo.mendez@cucei.udg.mx.
authors: Hugo O
authors: HO
authors: Méndez-Acosta
mesh_terms: D027882:Agave / Q000737:chemistry*; D000693:Anaerobiosis; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008697:Methane / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D008697:Methane; D006851:Hydrochloric Acid
keywords: Anaerobic digestion; Biogas production; Lignocellulosic biomass pretreatment; Response surface methodology; Sequencing batch reactor