title: Camelid nanobodies with high affinity for broad bean mottle virus: a possible promising tool to immunomodulate plant resistance against viruses.
abstract: Worldwide, plant viral infections decrease seriously the crop production yield, boosting the demand to develop new strategies to control viral diseases. One of these strategies to prevent viral infections, based on the immunomodulation faces many problems related to the ectopic expression of specific antibodies in planta. Camelid nanobodies, expressed in plants, may offer a solution as they are an attractive tool to bind efficiently to viral epitopes, cryptic or not accessible to conventional antibodies. Here, we report a novel, generic approach that might lead to virus resistance based on the expression of camelid specific nanobodies against Broad bean mottle virus (BBMV). Eight nanobodies, recognizing BBMV with high specificity and affinity, were retrieved after phage display from a large 'immune' library constructed from an immunized Arabic camel. By an in vitro assay we demonstrate how three nanobodies attenuate the BBMV spreading in inoculated Vicia faba plants. Furthermore, the in planta transient expression of these three selected nanobodies confirms their virus neutralizing capacity. In conclusion, this report supports that plant resistance against viral infections can be achieved by the in vivo expression of camelid nanobodies.
authors: Division of Plant Pathology, Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria, ascientific@aec.org.sy.
authors: Ahmed
authors: A
authors: Ghannam
authors: Safa
authors: S
authors: Kumari
authors: Serge
authors: S
authors: Muyldermans
authors: Abdul Qader
authors: AQ
authors: Abbady
mesh_terms: D060467:Disease Resistance; D007887:Fabaceae / Q000821:virology*; D010942:Plant Viruses / Q000276:immunology* / Q000502:physiology*; D061905:Single-Domain Antibodies / Q000276:immunology* / Q000378:metabolism*
chemical_list: D061905:Single-Domain Antibodies
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