title: Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples.
abstract: We describe a novel continuous-flow cell concentrator microdevice based on dielectrophoresis, and its associated custom-made control unit. The performances of a classical interdigitated metal electrode-based dielectrophoresis microfluidic device and this enhanced version, that includes insulator-based pole structures, were compared using the same setup. Escherichia coli samples were concentrated at several continuous flows and the device's trapping efficiencies were evaluated by exhaustive cell counts. Our results show that pole structures enhance the retention up to 12.6%, obtaining significant differences for flow rates up to 20 μL/min, when compared to an equivalent classical interdigitated electrodes setup. In addition, we performed a subsequent proteomic analysis to evaluate the viability of the biological samples after the long exposure to the actuating electrical field. No Escherichia coli protein alteration in any of the two systems was observed.
authors: Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.
authors: Beatriz
authors: B
authors: del Moral Zamora
authors: Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Juan Manuel
authors: JM
authors: Álvarez Azpeitia
authors: Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Ana Maria
authors: AM
authors: Oliva Brañas
authors: Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.
authors: Jordi
authors: J
authors: Colomer-Farrarons
authors: Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors: Marc
authors: M
authors: Castellarnau
authors: Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.
authors: Pere Ll
authors: PL
authors: Miribel-Català
authors: Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.|Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors: Antoni
authors: A
authors: Homs-Corbera
authors: Microbial Biotechnology and Host-Pathogen Interaction, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.
authors: Antonio
authors: A
authors: Juárez
authors: Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.|Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors: Josep
authors: J
authors: Samitier
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D040901:Proteomics / Q000295:instrumentation
keywords: Concentrator; Dielectrophoresis; Escherichia coli; Lab-on-a-chip