title: Molecular investigation of 2013 dengue Fever outbreak from delhi, India.
abstract: Dengue fever is a self-limiting, acute febrile disease which may aggravate to haemorrhage, plasma leakage and organ impairment in small number of cases. An outbreak of dengue fever occurred in Delhi, India after rainy season in the year 2013. Dengue virus specific RT-PCR was carried out on 378 suspected blood samples that were collected during the outbreak. Dengue virus was detected in 71% samples with highest number of patients infected by DENV-2 (86%) followed by DENV-1 (19 %) and DENV-3 (8%). Co-infection with more than one DENV serotype was detected in 14% samples. Twenty nine DENV strains (10 DENV-1, 12 DENV-2 and 7 DENV-3) were sequenced for partial envelope protein gene. Phylogenetic analysis grouped DENV-1 strains in the American African genotype, DENV-2 strains in the Cosmopolitan genotype and DENV-3 in Genotype III. We report the serotype distribution, circulating genotypes and partial envelope protein gene sequence of 29 DENV strains detected during 2013 outbreak in Delhi, India.
authors: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
authors: Nazia
authors: N
authors: Afreen
authors: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
authors: Farah
authors: F
authors: Deeba
authors: Dr. M. A. Ansari Health Centre, Jamia Millia Islamia, New Delhi, India.
authors: Irshad
authors: I
authors: Naqvi
authors: Dr. M. A. Ansari Health Centre, Jamia Millia Islamia, New Delhi, India.
authors: Mohammad
authors: M
authors: Shareef
authors: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors: Anwar
authors: A
authors: Ahmed
authors: Department of Microbiology, Jamia Hamdard, New Delhi, India.
authors: Shobha
authors: S
authors: Broor
authors: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
authors: Shama
authors: S
authors: Parveen
references: Infect Genet Evol. 2011 Aug;11(6):1443-8
references: 21632029
references: Emerg Infect Dis. 1999 Jul-Aug;5(4):589-90
references: 10458971
references: PLoS Negl Trop Dis. 2011 Nov;5(11):e1394
references: 22087347
references: Western Pac Surveill Response J. 2013 Jul 30;4(3):28-33
references: 24319611
references: Infect Genet Evol. 2008 Mar;8(2):191-204
references: 18243816
references: Trop Med Int Health. 2011 Aug;16(8):949-54
references: 21668590
references: J Clin Microbiol. 1992 Mar;30(3):545-51
references: 1372617
references: Southeast Asian J Trop Med Public Health. 2005 Sep;36(5):1174-8
references: 16438142
references: PLoS One. 2011;6(12):e29555
references: 22216313
references: Vaccine. 2012 Jun 19;30(29):4301-6
references: 22682286
references: Infect Genet Evol. 2013 Jun;16:401-10
references: 23523598
references: Indian J Exp Biol. 2010 Aug;48(8):849-57
references: 21341545
references: Arch Virol. 2008;153(12):2225-32
references: 19011729
references: Emerg Infect Dis. 2006 Feb;12(2):352-3
references: 17080580
references: Indian J Med Res. 1969 Apr;57(4):767-74
references: 5805380
references: Epidemiol Infect. 2012 Jul;140(7):1337-42
references: 21906409
references: Arch Virol. 2005 Mar;150(3):415-25
references: 15578237
references: Virol J. 2011 Jun 26;8:322
references: 21703015
references: Indian J Med Res. 1985 Oct;82:271-5
references: 4077169
references: Bull World Health Organ. 1992;70(1):105-8
references: 1568274
references: J Clin Virol. 2007 Sep;40(1):87-8
references: 17631046
references: Infect Genet Evol. 2012 Dec;12(8):1759-69
references: 22890284
references: Virol J. 2008 Jan 09;5:1
references: 18182120
references: Emerg Infect Dis. 2004 Apr;10(4):593-7
references: 15200847
references: Arch Virol. 2013 Mar;158(3):571-81
references: 23129129
references: PLoS One. 2013 Oct 21;8(10):e78030
references: 24205075
references: Am J Trop Med Hyg. 2007 Nov;77(5):903-9
references: 17984351