title: Mild hypothermia combined with a scaffold of NgR-silenced neural stem cells/Schwann cells to treat spinal cord injury.
abstract: Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differentiation, a cell-scaffold complex seeded with Nogo receptor (NgR)-silenced neural stem cells and Schwann cells may be able to improve the microenvironment for spinal cord injury repair. Previous studies have found that mild hypothermia helps to attenuate secondary damage in the spinal cord and exerts a neuroprotective effect. Here, we constructed a cell-scaffold complex consisting of a poly(D,L-lactide-co-glycolic acid) (PLGA) scaffold seeded with NgR-silenced neural stem cells and Schwann cells, and determined the effects of mild hypothermia combined with the cell-scaffold complexes on the spinal cord hemi-transection injury in the T9 segment in rats. Compared with the PLGA group and the NgR-silencing cells + PLGA group, hindlimb motor function and nerve electrophysiological function were clearly improved, pathological changes in the injured spinal cord were attenuated, and the number of surviving cells and nerve fibers were increased in the group treated with the NgR-silenced cell scaffold + mild hypothermia at 34°C for 6 hours. Furthermore, fewer pathological changes to the injured spinal cord and more surviving cells and nerve fibers were found after mild hypothermia therapy than in injuries not treated with mild hypothermia. These experimental results indicate that mild hypothermia combined with NgR gene-silenced cells in a PLGA scaffold may be an effective therapy for treating spinal cord injury.
authors: Department of Neurosurgery, the Fourth Center Clinical College of Tianjin Medical University, Tianjin Fourth Central Hospital, Tianjin, China.
authors: Dong
authors: D
authors: Wang
authors: Department of Clinical Detection, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang, Heilongjiang Province, China.
authors: Jinhua
authors: J
authors: Liang
authors: Department of Neurosurgery, the Fourth Center Clinical College of Tianjin Medical University, Tianjin Fourth Central Hospital, Tianjin, China.
authors: Jianjun
authors: J
authors: Zhang
authors: Department of Epidemiology, Logistics University of People's Armed Police Force, Tianjin, China.
authors: Shuhong
authors: S
authors: Liu
authors: Department of Neurosurgery, the Fourth Center Clinical College of Tianjin Medical University, Tianjin Fourth Central Hospital, Tianjin, China.
authors: Wenwen
authors: W
authors: Sun
keywords: Schwann cells; cell scaffold; mild hypothermia; nerve regeneration; neural regeneration; neural stem cells; neurological function; poly(D,L-lactide-co-glycolic acid); spinal cord injury
references: Asian Pac J Trop Med. 2014 Jul;7(7):562-7
references: 25063287
references: PLoS Biol. 2013 Dec;11(12):e1001738
references: 24358022
references: PLoS One. 2014 Apr 01;9(4):e93487
references: 24690752
references: Neurosurgery. 2005 Nov;57(5):1014-25; discussion 1014-25
references: 16284571
references: Spine (Phila Pa 1976). 1996 Oct 1;21(19):2190-6
references: 8902962
references: Prog Brain Res. 2002;137:231-55
references: 12440371
references: Spine (Phila Pa 1976). 2013 Aug 1;38(17):E1065-74
references: 23629485
references: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Jan;22(1):28-31
references: 20092707
references: Neurosci Lett. 2008 Sep 26;443(1):46-50
references: 18662744
references: J Biomed Mater Res A. 2015 Apr;103(4):1533-45
references: 25046856
references: Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3024-9
references: 11867737
references: J Neuroimmunol. 2013 Oct 15;263(1-2):83-90
references: 23993653
references: Exp Toxicol Pathol. 2011 Jan;63(1-2):151-6
references: 20005688
references: Glia. 2007 Jul;55(9):976-1000
references: 17526000
references: Exp Neurol. 2010 Apr;222(2):304-17
references: 20083105
references: Adv Exp Med Biol. 2012;760:53-73
references: 23281513
references: Chin J Traumatol. 2014;17(3):157-64
references: 24889979
references: J Neurotrauma. 2012 Mar 20;29(5):990-9
references: 21545262
references: Arch Iran Med. 2013 Sep;16(9):533-41
references: 23981158
references: Brain Res. 2009 Feb 23;1256:149-61
references: 19103176
references: Korean J Physiol Pharmacol. 2012 Dec;16(6):405-11
references: 23269903
references: Neuropathol Appl Neurobiol. 2011 Feb;37(2):135-55
references: 20819171
references: PLoS One. 2013 Aug 09;8(8):e71317
references: 23951131
references: Neuropathology. 2010 Dec;30(6):565-73
references: 20337950
references: PLoS Biol. 2008 Jul 22;6(7):e182
references: 18651793
references: Ther Hypothermia Temp Manag. 2014 Sep;4(3):115-24
references: 25019643
references: Neuroscience. 2006 Sep 29;142(1):287-304
references: 17120358
references: Unfallchirurg. 2009 Dec;112(12):1055-61
references: 19998020
references: Brain. 2007 Aug;130(Pt 8):2159-74
references: 17626035
references: Spinal Cord. 2013 May;51(5):395-400
references: 23247015
references: Spine (Phila Pa 1976). 2012 Dec 1;37(25):E1551-9
references: 22926281
references: Exp Neurol. 2014 Aug;258:35-47
references: 25017886