title: Ultrasonic vocalization changes and FOXP2 expression after experimental stroke.
abstract: Speech impairments affect one in four stroke survivors. However, animal models of post-ischemic vocalization deficits are limited. Male mice vocalize at ultrasonic frequencies when exposed to an estrous female mouse. In this study we assessed vocalization patterns and quantity in male mice after cerebral ischemia. FOXP2, a gene associated with verbal dyspraxia in humans, with known roles in neurogenesis and synaptic plasticity, was also examined after injury. Using a transient middle cerebral artery occlusion (MCAO) model, we assessed correlates of vocal impairment at several time-points after stroke. Further, to identify possible lateralization of vocalization deficits induced by left and right hemispheric strokes were compared. Significant differences in vocalization quantity were observed between stroke and sham animals that persisted for a month after injury. Injury to the left hemisphere reduced early vocalizations more profoundly than those to the right hemisphere. Nuclear expression of Foxp2 was elevated early after stroke (at 6h), but significantly decreased 24h after injury in both the nucleus and the cytoplasm. Neuronal Foxp2 expression increased in stroke mice compared to sham animals 4 weeks after injury. This study demonstrates that quantifiable deficits in ultrasonic vocalizations (USVs) are seen after stroke. USV may be a useful tool to assess chronic behavioral recovery in murine models of stroke.
authors: Departments of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030.
authors: Sarah J
authors: SJ
authors: Doran
authors: Departments of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030.
authors: Cassandra
authors: C
authors: Trammel
authors: Departments of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030.
authors: Sharon E
authors: SE
authors: Benashaski
authors: Departments of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030.
authors: Venugopal Reddy
authors: VR
authors: Venna
authors: Departments of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030; Departments of Neurology, University of Connecticut Health Center, Farmington, CT 06030. Electronic address: lmcullough@uchc.edu.
authors: Louise D
authors: LD
authors: McCullough
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D007839:Functional Laterality; D020244:Infarction, Middle Cerebral Artery; D007823:Laryngeal Nerves / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000473:pathology / Q000502:physiology; D011897:Random Allocation; D012097:Repressor Proteins / Q000378:metabolism*; D020521:Stroke / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D014465:Ultrasonics; D014828:Vocalization, Animal / Q000502:physiology*
chemical_list: D051858:Forkhead Transcription Factors; C432918:Foxp2 protein, mouse; D012097:Repressor Proteins
keywords: FOXP2/Foxp2 gene (in humans and other mammals; Middle cerebral artery occlusion; Ultrasonic vocalization; respectively)
references: PLoS One. 2014;9(1):e85405
references: 24416405
references: Cerebrovasc Dis. 2004;17(1):35-43
references: 14530636
references: Behav Brain Res. 2005 Mar 30;158(2):269-75
references: 15698893
references: J Stroke Cerebrovasc Dis. 2014 Nov-Dec;23(10):e477-8
references: 25284718
references: Science. 2013 Jun 14;340(6138):1230531
references: 23766329
references: Stroke. 2009 May;40(5):1842-8
references: 19265047
references: Neurosci Res. 1995 Mar;22(1):23-30
references: 7792080
references: Nat Rev Neurosci. 2005 Feb;6(2):131-8
references: 15685218
references: Methods Mol Biol. 2014;1135:81-93
references: 24510856
references: Stroke. 1989 Jan;20(1):84-91
references: 2643202
references: Exp Neurol. 2012 Dec;238(2):114-21
references: 22921463
references: Nat Genet. 1998 Feb;18(2):168-70
references: 9462748
references: J Neurosci. 2013 Jan 2;33(1):244-58
references: 23283338
references: Behav Brain Res. 2015 Mar 15;281:78-85
references: 25523030
references: Behav Brain Res. 2014 Mar 1;260:162-70
references: 24211537
references: PLoS One. 2012;7(1):e29401
references: 22238608
references: Brain. 2006 Jun;129(Pt 6):1371-84
references: 16638796
references: PLoS One. 2012;7(3):e33130
references: 22412993
references: Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9643-8
references: 15983371
references: Neurosci Lett. 2014 Apr 30;566:162-6
references: 24607928
references: Neurosci Biobehav Rev. 2014 Jun;43:191-8
references: 24769292
references: Eur J Hum Genet. 2012 Feb;20(2):224-9
references: 21897444
references: Acta Neuropathol. 2012 Sep;124(3):425-38
references: 22562356
references: J Neurosci. 2000 Feb 15;20(4):1559-67
references: 10662845
references: Am J Phys Anthropol. 2002;Suppl 35:36-62
references: 12653308
references: Curr Biol. 2008 Mar 11;18(5):354-62
references: 18328704
references: PLoS Biol. 2005 Dec;3(12):e386
references: 16248680
references: J Neurosci. 2013 Feb 20;33(8):3276-83
references: 23426656
references: PLoS One. 2010;5(11):e14115
references: 21124767
references: Transl Psychiatry. 2014;4:e351
references: 24473442
references: J Am Assoc Lab Anim Sci. 2007 Jan;46(1):28-34
references: 17203913
references: Transl Stroke Res. 2014 Oct;5(5):577-85
references: 24838614
references: Stroke. 2010 Nov;41(11):2645-52
references: 20847317
references: Genesis. 2007 Jul;45(7):440-6
references: 17619227
references: BMC Neurol. 2014;14:46
references: 24607148
references: Front Psychol. 2014 Sep 23;5:1033
references: 25295017
references: Curr Opin Neurobiol. 2014 Oct;28:72-9
references: 25048596
references: Front Behav Neurosci. 2013 Nov 19;7:159
references: 24312027
references: Front Behav Neurosci. 2014 Nov 18;8:399
references: 25477796
references: Behav Brain Res. 2008 Jul 19;190(2):243-7
references: 18417226
references: Proc Natl Acad Sci U S A. 2014 Sep 30;111(39):14253-8
references: 25225386
references: Cochrane Database Syst Rev. 2012;5:CD000425
references: 22592672