title: SiRNA directed against annexin II receptor inhibits angiogenesis via suppressing MMP2 and MMP9 expression.
abstract: OBJECTIVE
  Annexin II receptor (AXIIR) is able to mediate Annexin II signal and induce apoptosis, but its role in angiogenesis remains unclear. This study tries to investigate the role of AXIIR in angiogenesis and the plausible molecular mechanism.
  
  
  RESULTS
  RNA interference technology was used to silence AXIIR, and the subsequent effects in vitro and in vivo were evaluated thereafter. Our data indicated that human umbilical vein endothelial cells (HUVECs) expressed AXIIR and knockdown of AXIIR significantly inhibited HUVECs proliferation, adhesion, migration, and tube formation in vitro and suppressed angiogenesis in vivo. Furthermore, AXIIR siRNA induced cell arrest in the S/G2 phase while had no effect on cell apoptosis. We found that these subsequent effects might be via suppressing the expression of matrix metalloproteinase 2and matrix metalloproteinase 9.
  
  
  CONCLUSIONS
  AXIIR participates in angiogenesis, and may be a potential therapeutic target for angiogenesis related diseases.
authors: Department of Ophthalmology, Changhai Hospital, Second Military University, Shanghai, China.
authors: Hongyuan
authors: H
authors: Song
authors: Dongyan
authors: D
authors: Pan
authors: Weifeng
authors: W
authors: Sun
authors: Cao
authors: C
authors: Gu
authors: Yuelu
authors: Y
authors: Zhang
authors: Ping
authors: P
authors: Zhao
authors: Zhongtian
authors: Z
authors: Qi
authors: Shihong
authors: S
authors: Zhao
mesh_terms: D017306:Annexin A2 / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic / Q000235:genetics*; D010766:Phosphorylation; D034741:RNA, Small Interfering; D018000:Receptors, Peptide / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D017306:Annexin A2; D034741:RNA, Small Interfering; D018000:Receptors, Peptide; C514873:annexin II receptor, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9