title: Two cinnamoyloctopamine antioxidants from garlic skin attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis.
abstract: Hepatic oxidative stress plays a key role in the development of non-alcoholic steatohepatitis (NASH), therefore, treatment approaches that address the antioxidant is helpful in the therapy of patients with NASH. N-trans-coumaroyloctopamine (1) and N-trans-feruloyloctopamine (2) were identified as the primary antioxidant constituents of garlic skin with high antioxidant activities. The aim of this study was to elucidate the protective effect and mechanism of the antioxidants on NASH in rats. The results provide morphological and molecular biological evidences for the protective role of the antioxidant 2 in ameliorating oxidative stress and hepatic apoptosis in experimental NASH for the first time. Mechanism study indicated that the antioxidant 2 significantly reduced the expression of COX-2 mRNA and protein by western blot, RT-PCR and immunohistochemical techniques.
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Zheng-Rong
authors: ZR
authors: Wu
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Peng-Chen
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Yang-Li
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Jian-Ying
authors: JY
authors: Li
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Xin-Wang
authors: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China.
authors: Yong-Wang
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Ding-Ding
authors: DD
authors: Guo
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Lei-Cui
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China.
authors: Qian-Guo
authors: QG
authors: Guan
authors: School of Pharmaceutics, Lanzhou University, People's Republic of China; Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China. Electronic address: lihy@lzu.edu.cn.
authors: Hong-Yu
authors: HY
authors: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005737:Garlic / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D015394:Molecular Structure; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy*; D009655:Octopamine / Q000031:analogs & derivatives / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; C498173:N-feruloyloctopamine; D009655:Octopamine; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: Antioxidant; COX-2; Garlic skin; Hepatic oxidative stress; N-trans-Feruloyloctopamine; Nonalcoholic steatohepatitis