title: Auxin-induced degradation dynamics set the pace for lateral root development.
abstract: Auxin elicits diverse cell behaviors through a simple nuclear signaling pathway initiated by degradation of Aux/IAA co-repressors. Our previous work revealed that members of the large Arabidopsis Aux/IAA family exhibit a range of degradation rates in synthetic contexts. However, it remained an unresolved issue whether differences in Aux/IAA turnover rates played a significant role in plant responses to auxin. Here, we use the well-established model of lateral root development to directly test the hypothesis that the rate of auxin-induced Aux/IAA turnover sets the pace for auxin-regulated developmental events. We did this by generating transgenic plants expressing degradation rate variants of IAA14, a crucial determinant of lateral root initiation. Progression through the well-established stages of lateral root development was strongly correlated with the engineered rates of IAA14 turnover, leading to the conclusion that Aux/IAAs are auxin-initiated timers that synchronize developmental transitions.
authors: Department of Biology, University of Washington, Seattle, WA 98195, USA.
authors: Jessica M
authors: JM
authors: Guseman
authors: Leibniz Institute of Plant Biochemistry, Halle (Saale) 06120, Germany.
authors: Antje
authors: A
authors: Hellmuth
authors: Department of Biology, University of Washington, Seattle, WA 98195, USA.
authors: Amy
authors: A
authors: Lanctot
authors: Department of Biology, University of Washington, Seattle, WA 98195, USA.
authors: Tamar P
authors: TP
authors: Feldman
authors: Department of Biology, University of Washington, Seattle, WA 98195, USA.
authors: Britney L
authors: BL
authors: Moss
authors: Department of Electrical Engineering, University of Washington, Seattle, WA 98195, USA.
authors: Eric
authors: E
authors: Klavins
authors: Leibniz Institute of Plant Biochemistry, Halle (Saale) 06120, Germany.
authors: Luz Irina A
authors: LI
authors: Calderón Villalobos
authors: Department of Biology, University of Washington, Seattle, WA 98195, USA jn7@uw.edu.
authors: Jennifer L
authors: JL
authors: Nemhauser
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Arabidopsis; Phytohormone; Saccharomyces; Ubiquitin
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