title: Dietary CML-enriched protein induces functional arterial aging in a RAGE-dependent manner in mice.
abstract: METHODS
  Advanced glycation end-products (AGEs) are endogenously produced and are present in food. N(ε)-carboxymethyllysine (CML) is an endothelial activator via the receptor for AGEs (RAGEs) and is a major dietary AGE. This work investigated the effects of a CML-enriched diet and RAGE involvement in aortic aging in mice.
  
  
  RESULTS
  After 9 months of a control diet or CML-enriched diets (50, 100, or 200 μg(CML)/g of food), endothelium-dependent relaxation, RAGE, vascular cell adhesion molecule-1, and sirtuin-1 expression, pulse wave velocity and elastin disruption were measured in aortas of wild-type or RAGE(-/-) male C57BL/6 mice. Compared to the control diet, endothelium-dependent relaxation was reduced in the wild-type mice fed the CML-enriched diet (200 μg(CML)/g) (66.8 ± 12.26 vs. 94.3 ± 2.6%, p < 0.01). RAGE and vascular cell adhesion molecule-1 (p < 0.05) expression were increased in the aortic wall. RAGE(-/-) mice were protected against CML-enriched diet-induced endothelial dysfunction. Compared to control diet, the CML-enriched diet (200 μg(CML)/g) increased the aortic pulse wave velocity (86.6 ± 41.1 vs. 251.4 ± 41.1 cm/s, p < 0.05) in wild-type animals. Elastin disruption was found to a greater extent in the CML-fed mice (p < 0.05). RAGE(-/-) mice fed the CML-enriched diet were protected from aortic stiffening.
  
  
  CONCLUSIONS
  Chronic CML ingestion induced endothelial dysfunction, arterial stiffness and aging in a RAGE-dependent manner.
authors: Inserm U995/Team "Glycation: from inflammation to aging", Lille School of Medicine, Lille University, Lille, France.
authors: Nicolas
authors: N
authors: Grossin
authors: Florent
authors: F
authors: Auger
authors: Céline
authors: C
authors: Niquet-Leridon
authors: Nicolas
authors: N
authors: Durieux
authors: David
authors: D
authors: Montaigne
authors: Ann Marie
authors: AM
authors: Schmidt
authors: Sophie
authors: S
authors: Susen
authors: Philippe
authors: P
authors: Jacolot
authors: Jean-Baptiste
authors: JB
authors: Beuscart
authors: Frédéric J
authors: FJ
authors: Tessier
authors: Eric
authors: E
authors: Boulanger
mesh_terms: D000375:Aging; D000818:Animals; D001158:Arteries / Q000502:physiology*; D004044:Dietary Proteins / Q000008:administration & dosage*; D004730:Endothelium, Vascular / Q000502:physiology; D008239:Lysine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D063177:Pulse Wave Analysis; D000067759:Receptor for Advanced Glycation End Products / Q000502:physiology*; D056564:Sirtuin 1 / Q000032:analysis; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis; D059289:Vascular Stiffness
chemical_list: D004044:Dietary Proteins; D000067759:Receptor for Advanced Glycation End Products; D019010:Vascular Cell Adhesion Molecule-1; C048496:N(6)-carboxymethyllysine; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D008239:Lysine
keywords: Aging; Aorta; Endothelium; Glycation; Nutrition