title: Prevalence of Treponema spp. in endodontic retreatment-resistant periapical lesions.
abstract: This study investigated the presence of the Treponema species in longstanding endodontic retreatment-resistant lesions of teeth with apical periodontitis, the association of this species with clinical/radiographic features, and the association among the different target species. Microbial samples of apical lesions were collected from twenty-five adult patients referred to endodontic surgery after unsuccessful root canal retreatment. Nested-PCR and conventional PCR were used for Treponema detection. Twenty-three periradicular tissue samples showed detectable levels of bacterial DNA. Treponema species were detected in 28% (7/25) of the cases. The most frequently detected species were T. socranskii (6/25), followed by T. maltophilum (3/25), T. amylovorum (3/25), T. lecithinolyticum (3/25), T. denticola (3/25), T. pectinovorum (2/25) and T. medium (2/25). T. vicentii was not detected in any sample. Positive statistical association was found between T. socranskii and T. denticola, and between T. maltophilum and T. lecithinolyticum . No association was detected between the presence of any target microorganism and the clinical or radiographic features. Treponema spp. are present, in a low percentage, in longstanding apical lesions from teeth with endodontic retreatment failure.
authors: Department of Restorative Dentistry, Piracicaba Dental School, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
authors: Tiago Pereira
authors: TP
authors: Rosa
authors: Department of Restorative Dentistry, Piracicaba Dental School, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
authors: Fernanda Graziela Corrêa
authors: FG
authors: Signoretti
authors: Department of Conservative Dentistry, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Francisco
authors: F
authors: Montagner
authors: Department of Restorative Dentistry, Piracicaba Dental School, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
authors: Brenda Paula Figueiredo de Almeida
authors: BP
authors: Gomes
authors: School of Dentistry, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: Rogério Castilho
authors: RC
authors: Jacinto
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000473:pathology / Q000628:therapy*; D010486:Periapical Tissue / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011859:Radiography; D019233:Retreatment; D012390:Root Canal Therapy; D017211:Treatment Failure; D014208:Treponema / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000000981:diagnostic imaging / Q000382:microbiology*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial