title: A real-time PCR for detection and quantification of Mycoplasma ovipneumoniae.
abstract: A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 10(2) to 2.2 × 10(7) genomes.
authors: Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China.
authors: Falong
authors: F
authors: Yang
authors: Xiaofang
authors: X
authors: Dao
authors: Alex
authors: A
authors: Rodriguez-Palacios
authors: Xufei
authors: X
authors: Feng
authors: Cheng
authors: C
authors: Tang
authors: Xiaonong
authors: X
authors: Yang
authors: Hua
authors: H
authors: Yue
mesh_terms: D000818:Animals; D001483:Base Sequence; D052160:Benzothiazoles; D002681:China; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D003959:Diamines; D016680:Genome, Bacterial / Q000235:genetics; D006041:Goats / Q000382:microbiology*; D008969:Molecular Sequence Data; D045802:Mycoplasma ovipneumoniae / Q000235:genetics*; D009930:Organic Chemicals; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I
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