title: Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes.
abstract: A method of immobilizing clenbuterol (CLEN) on the sensor chip for spectral surface plasmon resonance imaging (SPRi) was experimentally investigated. The bioprobes on the sensor chip were prepared by immobilizing bovine serum albumin (BSA) protein and conjugating CLEN molecules to BSA, which provides more active points and free orientations for specific binding. The calibration curve showed that the wavelength resonance shift decreased as the concentration of CLEN analyte increased, consistent with the inhibition principle. The limit of detection (LOD) was estimated to be 6.32 μg/ml. This method proved to be highly specific, high throughput, label free, and operationally convenient.
authors: Functional Materials Research Laboratory, Tongji University, Shanghai 200092, People's Republic of China.
authors: Manwen
authors: M
authors: Yao
authors: Functional Materials Research Laboratory, Tongji University, Shanghai 200092, People's Republic of China.
authors: Yichuan
authors: Y
authors: Wu
authors: School of Science, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. Electronic address: xyfang@mail.xjtu.edu.cn.
authors: Xiangyi
authors: X
authors: Fang
authors: Department of Clinical Laboratory, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, People's Republic of China.
authors: Yucong
authors: Y
authors: Yang
authors: School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.
authors: Hongjun
authors: H
authors: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D002976:Clenbuterol / Q000032:analysis*; D015335:Molecular Probes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D015335:Molecular Probes; D012710:Serum Albumin, Bovine; D002976:Clenbuterol
keywords: Bioprobe immobilization; Clenbuterol detection; Surface plasmon resonance imaging (SPRi)