title: Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles.
abstract: Bismuth Ferrite (BFO) nanoparticles (BFO-NP) display interesting optical (nonlinear response) and magnetic properties which make them amenable for bio-oriented diagnostic applications as intra- and extra membrane contrast agents. Due to the relatively recent availability of this material in well dispersed nanometric form, its biocompatibility was not known to date. In this study, we present a thorough assessment of the effects of in vitro exposure of human adenocarcinoma (A549), lung squamous carcinoma (NCI-H520), and acute monocytic leukemia (THP-1) cell lines to uncoated and poly(ethylene glycol)-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility. Our results support the attractiveness of the functional-BFO towards biomedical applications focused on advanced diagnostic imaging.
  
  
  BACKGROUND
  Bismuth Ferrite nanoparticles (BFO-NP) have been recently successfully introduced as photodynamic tools and imaging probes. However, how these nanoparticles interact with various cells at the cellular level remains poorly understood. In this study, the authors performed in vitro experiments to assess the effects of uncoated and PEG-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility.
authors: Institute of Chemical Sciences and Engineering, EPFL, Batochime, 1015, Lausanne, Switzerland.
authors: Davide
authors: D
authors: Staedler
authors: Institute of Chemical Sciences and Engineering, EPFL, Batochime, 1015, Lausanne, Switzerland.
authors: Solène
authors: S
authors: Passemard
authors: GAP-Biophotonics, Université de Genève, 22 Chemin de Pinchat, 1211 Genève 4, Switzerland.
authors: Thibaud
authors: T
authors: Magouroux
authors: GAP-Biophotonics, Université de Genève, 22 Chemin de Pinchat, 1211 Genève 4, Switzerland.
authors: Andrii
authors: A
authors: Rogov
authors: Nanomedicine Laboratory and Molecular Imaging Group, School of Medicine, Trinity Centre for Health Sciences, Trinity College, D8, Dublin, Ireland.
authors: Ciaran Manus
authors: CM
authors: Maguire
authors: Nanomedicine Laboratory and Molecular Imaging Group, School of Medicine, Trinity Centre for Health Sciences, Trinity College, D8, Dublin, Ireland.
authors: Bashir M
authors: BM
authors: Mohamed
authors: FEE Gmbh, Struthstrasse 2, 55743 Idar-Oberstein, Germany.
authors: Sebastian
authors: S
authors: Schwung
authors: FEE Gmbh, Struthstrasse 2, 55743 Idar-Oberstein, Germany.
authors: Daniel
authors: D
authors: Rytz
authors: Fachbereich Chemieingenieurwesen, Fachhochschule Münster, Stegerwaldstrasse 39, 48565 Steinfurt, Germany.
authors: Thomas
authors: T
authors: Jüstel
authors: GAP-Biophotonics, Université de Genève, 22 Chemin de Pinchat, 1211 Genève 4, Switzerland.
authors: Stéphanie
authors: S
authors: Hwu
authors: Univ. Savoie, SYMME, F-74000, Annecy, France.
authors: Yannick
authors: Y
authors: Mugnier
authors: Univ. Savoie, SYMME, F-74000, Annecy, France.
authors: Ronan
authors: R
authors: Le Dantec
authors: Nanomedicine Laboratory and Molecular Imaging Group, School of Medicine, Trinity Centre for Health Sciences, Trinity College, D8, Dublin, Ireland; AMBER Centre and CRANN Institute, Trinity College, D2, Dublin, Ireland.
authors: Yuri
authors: Y
authors: Volkov
authors: Institute of Chemical Sciences and Engineering, EPFL, Batochime, 1015, Lausanne, Switzerland.
authors: Sandrine
authors: S
authors: Gerber-Lemaire
authors: Nanomedicine Laboratory and Molecular Imaging Group, School of Medicine, Trinity Centre for Health Sciences, Trinity College, D8, Dublin, Ireland; AMBER Centre and CRANN Institute, Trinity College, D2, Dublin, Ireland.
authors: Adriele
authors: A
authors: Prina-Mello
authors: GAP-Biophotonics, Université de Genève, 22 Chemin de Pinchat, 1211 Genève 4, Switzerland. Electronic address: luigi.bonacina@unige.ch.
authors: Luigi
authors: L
authors: Bonacina
authors: GAP-Biophotonics, Université de Genève, 22 Chemin de Pinchat, 1211 Genève 4, Switzerland.
authors: Jean-Pierre
authors: JP
authors: Wolf
mesh_terms: D001729:Bismuth / Q000737:chemistry*; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003287:Contrast Media / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D005290:Ferric Compounds; D001729:Bismuth
keywords: Biocompatibility; Bismuth ferrite; Nanophotonic; Non-linear imaging; PEGylation