title: Femtosecond laser bone ablation with a high repetition rate fiber laser source.
abstract: Femtosecond laser pulses can be used to perform very precise cutting of material, including biological samples from subcellular organelles to large areas of bone, through plasma-mediated ablation. The use of a kilohertz regenerative amplifier is usually needed to obtain the pulse energy required for ablation. This work investigates a 5 megahertz compact fiber laser for near-video rate imaging and ablation in bone. After optimization of ablation efficiency and reduction in autofluorescence, the system is demonstrated for the in vivo study of bone regeneration. Image-guided creation of a bone defect and longitudinal evaluation of cellular injury response in the defect provides insight into the bone regeneration process.
authors: Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA ; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Luke J
authors: LJ
authors: Mortensen
authors: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Clemens
authors: C
authors: Alt
authors: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
authors: Raphaël
authors: R
authors: Turcotte
authors: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Marissa
authors: M
authors: Masek
authors: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors: Tzu-Ming
authors: TM
authors: Liu
authors: Centre de Recherche Université Laval Robert-Giffard, Université Laval, Québec, QC G1J2G3, Canada.
authors: Daniel C
authors: DC
authors: Côté
authors: School of Applied and Engineering Physics, Cornell University, Ithaca, New York, USA.
authors: Chris
authors: C
authors: Xu
authors: Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA ; Harvard Stem Cell Institute, Cambridge, Massachusetts, USA ; Giuseppe_Intini@hsdm.harvard.edu ; Co-corresponding authors.
authors: Giuseppe
authors: G
authors: Intini
authors: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA ; Harvard Stem Cell Institute, Cambridge, Massachusetts, USA ; Co-corresponding authors.
authors: Charles P
authors: CP
authors: Lin
keywords: (060.4370) Nonlinear optics, fibers; (170.1020) Ablation of tissue; (170.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy
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