title: Scaffold-free three-dimensional culture systems for mass production of periosteum-derived progenitor cells.
abstract: Mesenchymal stem cells (MSCs) are capable of self-renewal and can differentiate into various types of cells for therapeutic purposes. MSCs are frequently cultured in a two-dimensional (2D) system. However, MSCs can lose their differentiation capacity over time in this culture system. In addition, the available surface area for the propagation of cells is limited. Therefore, various three-dimensional (3D) culture systems have been developed. In this study, we developed the scaffold-free 3D culture systems for the expansion of periosteum-derived progenitor cells (PDPCs) as spheres. The spheroid formation of PDPCs was induced using a rotation platform. The spheres maintained their viability and proliferation ability. Moreover, expression levels of the stemness marker genes and proteins were higher in cells grown on 3D culture system than in 2D culture system. In conclusion, a simple and economical 3D culture system has been developed that can increase the potential of PDPCs for clinical use.
authors: Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea.
authors: Hyun-Myoung
authors: HM
authors: Cha
authors: Department of Applied Bioscience, CHA University, Seongnam 463-836, Republic of Korea.
authors: Sun-Mi
authors: SM
authors: Kim
authors: Department of Applied Bioscience, CHA University, Seongnam 463-836, Republic of Korea.
authors: Yong-Soo
authors: YS
authors: Choi
authors: Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea. Electronic address: kimdi@inha.ac.kr.
authors: Dong-Il
authors: DI
authors: Kim
mesh_terms: D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010521:Periosteum / Q000166:cytology*; D012399:Rotation; D018874:Spheroids, Cellular / Q000166:cytology; D054457:Tissue Scaffolds
chemical_list: D015415:Biomarkers
keywords: Mesenchymal stem cells; Periosteum-derived progenitor cells; Scaffold-free culture; Spheroid formation; Three-dimensional culture system