title: Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution.
abstract: Synaptonemal complexes (SCs) are meiosis-specific multiprotein complexes that are essential for synapsis, recombination, and segregation of homologous chromosomes, but the molecular organization of SCs remains unclear. We used immunofluorescence labeling in combination with super-resolution imaging and average position determination to investigate the molecular architecture of SCs. Combination of 2D super-resolution images recorded from different areas of the helical ladder-like structure allowed us to reconstruct the 3D molecular organization of the mammalian SC with isotropic resolution. The central element is composed of two parallel cables at a distance of ∼ 100 nm, which are oriented perpendicular to two parallel cables of the lateral element arranged at a distance of ∼ 220 nm. The two parallel cable elements form twisted helical structures that are connected by transversal filaments by their N and C termini. A single-cell preparation generates sufficient localizations to compile a 3D model of the SC with nanometer precision.
authors: Departments of Cell and Developmental Biology and.
authors: Katharina
authors: K
authors: Schücker
authors: Biotechnology and Biophysics, Biocenter, University of Würzburg, 97074 Würzburg, Germany.
authors: Thorge
authors: T
authors: Holm
authors: Biotechnology and Biophysics, Biocenter, University of Würzburg, 97074 Würzburg, Germany.
authors: Christian
authors: C
authors: Franke
authors: Biotechnology and Biophysics, Biocenter, University of Würzburg, 97074 Würzburg, Germany m.sauer@uni-wuerzburg.de benavente@biozentrum.uni-wuerzburg.de.
authors: Markus
authors: M
authors: Sauer
authors: Departments of Cell and Developmental Biology and m.sauer@uni-wuerzburg.de benavente@biozentrum.uni-wuerzburg.de.
authors: Ricardo
authors: R
authors: Benavente
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D013573:Synaptonemal Complex / Q000648:ultrastructure*
keywords: average position determination; dSTORM; meiosis; super-resolution imaging; synaptonemal complex
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