title: Comparison of Vibratome and Compresstome sectioning of fresh primate lymphoid and genital tissues for in situ MHC-tetramer and immunofluorescence staining.
abstract: BACKGROUND
  For decades, the Vibratome served as a standard laboratory resource for sectioning fresh and fixed tissues. In skilled hands, high quality and consistent fresh unfixed tissue sections can be produced using a Vibratome but the sectioning procedure is extremely time consuming. In this study, we conducted a systematic comparison between the Vibratome and a new approach to section fresh unfixed tissues using a Compresstome. We used a Vibratome and a Compresstome to cut fresh unfixed lymphoid and genital non-human primate tissues then used in situ tetramer staining to label virus-specific CD8 T cells and immunofluorescent counter-staining to label B and T cells. We compared the Vibratome and Compresstome in five different sectioning parameters: speed of cutting, chilling capability, specimen stabilization, size of section, and section/staining quality.
  
  
  RESULTS
  Overall, the Compresstome and Vibratome both produced high quality sections from unfixed spleen, lymph node, vagina, cervix, and uterus, and subsequent immunofluorescent staining was equivalent. The Compresstome however, offered distinct advantages; producing sections approximately 5 times faster than the Vibratome, cutting tissue sections more easily, and allowing production of larger sections.
  
  
  CONCLUSIONS
  A Compresstome can be used to generate fresh unfixed primate lymph node, spleen, vagina, cervix and uterus sections, and is superior to a Vibratome in cutting these fresh tissues.
authors: Department of Veterinary and Biomedical Sciences, college of veterinary medicine, University of Minnesota, St. Paul, 1971 Commonwealth Avenue, Minnesota, MN 55108 USA ; Departments of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, 44519 Egypt.
authors: Hadia M
authors: HM
authors: Abdelaal
authors: Department of Veterinary and Biomedical Sciences, college of veterinary medicine, University of Minnesota, St. Paul, 1971 Commonwealth Avenue, Minnesota, MN 55108 USA.
authors: Hyeon O
authors: HO
authors: Kim
authors: Department of Veterinary and Biomedical Sciences, college of veterinary medicine, University of Minnesota, St. Paul, 1971 Commonwealth Avenue, Minnesota, MN 55108 USA.
authors: Reece
authors: R
authors: Wagstaff
authors: Department of Veterinary and Biomedical Sciences, college of veterinary medicine, University of Minnesota, St. Paul, 1971 Commonwealth Avenue, Minnesota, MN 55108 USA.
authors: Ryoko
authors: R
authors: Sawahata
authors: Departments of Microbiology, MMC 196, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455 USA.
authors: Peter J
authors: PJ
authors: Southern
authors: Department of Veterinary and Biomedical Sciences, college of veterinary medicine, University of Minnesota, St. Paul, 1971 Commonwealth Avenue, Minnesota, MN 55108 USA.
authors: Pamela J
authors: PJ
authors: Skinner
keywords: Cervix; Compresstome; Immunohistochemistry; Lymph node; Spleen; Unfixed fresh tissue sectioning; Uterus; Vagina; Vibratome; in situ tetramer staining
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