title: Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection.
abstract: H1N1 swine influenza A virus (H1N1 SwIV) is one key subtype of influenza viruses with pandemic potential. MicroRNAs (miRNAs) are endogenous small RNA molecules that regulate gene expression. MiRNAs relevant with H1N1 SwIV have rarely been reported. To understand the biological functions of miRNAs during H1N1 SwIV infection, this study profiled differentially expressed (DE) miRNAs in pulmonary alveolar macrophages from piglets during the H1N1 SwIV infection using a deep sequencing approach, which was validated by quantitative real-time PCR. Compared to control group, 70 and 16 DE miRNAs were respectively identified on post-infection day (PID) 4 and PID 7. 56 DE miRNAs were identified between PID 4 and PID 7. Our results suggest that most host miRNAs are down-regulated to defend the H1N1 SwIV infection during the acute phase of swine influenza whereas their expression levels gradually return to normal during the recovery phase to avoid the occurrence of too severe porcine lung damage. In addition, targets of DE miRNAs were also obtained, for which bioinformatics analyses were performed. Our results would be useful for investigating the functions and regulatory mechanisms of miRNAs in human influenza because pig serves as an excellent animal model to study the pathogenesis of human influenza.
authors: 1] College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China [2] Department of Microbiology &Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, P. R. China.
authors: Pengfei
authors: P
authors: Jiang
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Na
authors: N
authors: Zhou
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Xinyu
authors: X
authors: Chen
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Xing
authors: X
authors: Zhao
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Dengyun
authors: D
authors: Li
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Fen
authors: F
authors: Wang
authors: State Key Laboratory of Biology Macromolecule, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, P. R. China.
authors: Lijun
authors: L
authors: Bi
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Xi'an City, Shaanxi, 712100, P. R. China.
authors: Deli
authors: D
authors: Zhang
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology; D015536:Down-Regulation; D059014:High-Throughput Nucleotide Sequencing; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D016676:Macrophages, Alveolar / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D060066:Protein Interaction Maps; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology*
chemical_list: D035683:MicroRNAs
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