title: Suppressive effect of insulin on the gene expression and plasma concentrations of mediators of asthmatic inflammation.
abstract: OBJECTIVE
  Following our recent demonstration that the chronic inflammatory and insulin resistant state of obesity is associated with an increase in the expression of mediators known to contribute to the pathogenesis of asthma and that weight loss after gastric bypass surgery results in the reduction of these genes, we have now hypothesized that insulin suppresses the cellular expression and plasma concentrations of these mediators.
  
  
  METHODS
  The expression of IL-4, LIGHT, LTBR, ADAM-33, and TSLP in MNC and plasma concentrations of LIGHT, TGF-β1, MMP-9, MCP-1, TSLP, and NOM in obese patients with T2DM were measured before, during, and after the infusion of a low dose (2 U/h) infusion of insulin for 4 hours. The patients were also infused with dextrose or saline for 4 hours on two separate visits and served as controls. Results. Following insulin infusion, the mRNA expression of IL-4, ADAM-33, LIGHT, and LTBR mRNA expression fell significantly (P < 0.05 for all). There was also a concomitant reduction in plasma NOM, LIGHT, TGF-β1, MCP-1, and MMP-9 concentrations.
  
  
  CONCLUSIONS
  Insulin suppresses the expression of these genes and mediators related to asthma and may, therefore, have a potential role in the treatment of asthma.
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Husam
authors: H
authors: Ghanim
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Kelly
authors: 0000-0003-4827-3713
authors: K
authors: Green
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Sanaa
authors: S
authors: Abuaysheh
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Manav
authors: M
authors: Batra
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Nitesh D
authors: ND
authors: Kuhadiya
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Reema
authors: R
authors: Patel
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Antoine
authors: A
authors: Makdissi
authors: Division of Endocrinology and Metabolism, Texas Tech University Health Sciences Center, 701 W 5th Street, Odessa, TX 79763, USA.
authors: Sandeep
authors: S
authors: Dhindsa
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Ajay
authors: A
authors: Chaudhuri
authors: Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 115 Flint Road, Williamsville, NY 14221, USA.
authors: Paresh
authors: P
authors: Dandona
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D018592:Cross-Over Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000097:blood*; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000378:metabolism*; D007333:Insulin Resistance; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D017382:Reactive Oxygen Species; D015431:Weight Loss
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007328:Insulin; D017382:Reactive Oxygen Species; C517652:hemoglobin A1c protein, human
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