title: Molecular cloning and gene expression of canine apoptosis inhibitor of macrophage.
abstract: Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.
authors: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors: Shintaro
authors: S
authors: Tomura
authors: Mona
authors: M
authors: Uchida
authors: Tomohiro
authors: T
authors: Yonezawa
authors: Masato
authors: M
authors: Kobayashi
authors: Makoto
authors: M
authors: Bonkobara
authors: Satoko
authors: S
authors: Arai
authors: Toru
authors: T
authors: Miyazaki
authors: Satoshi
authors: S
authors: Tamahara
authors: Naoaki
authors: N
authors: Matsuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008969:Molecular Sequence Data; D051116:Receptors, Scavenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology; D013154:Spleen / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D051116:Receptors, Scavenger
references: J Clin Invest. 2000 Apr;105(8):1049-56
references: 10772649
references: Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12072-7
references: 21730133
references: Am J Pathol. 2003 Mar;162(3):837-47
references: 12598318
references: Proc Natl Acad Sci U S A. 1990 Nov;87(22):8810-4
references: 1978939
references: Trends Biochem Sci. 1992 Apr;17(4):141-6
references: 1585457
references: Trends Biochem Sci. 1994 Jan;19(1):5-8
references: 8140623
references: J Biol Chem. 1996 Oct 25;271(43):26924-30
references: 8900177
references: J Biol Chem. 1997 Mar 7;272(10):6151-8
references: 9045627
references: J Exp Med. 1999 Jan 18;189(2):413-22
references: 9892623
references: Eur J Immunol. 1999 Apr;29(4):1086-93
references: 10229074
references: Cell Metab. 2005 Mar;1(3):201-13
references: 16054063
references: J Clin Invest. 2006 Jan;116(1):33-5
references: 16395402
references: J Clin Invest. 2006 Jul;116(7):1793-801
references: 16823477
references: Vet Pathol. 2006 Sep;43(5):632-45
references: 16966440
references: J Vet Med Sci. 2006 Dec;68(12):1343-6
references: 17213706
references: Clin Chem. 2007 Oct;53(10):1792-9
references: 17702858
references: Exp Mol Med. 2008 Dec 31;40(6):677-85
references: 19116453
references: BMC Cancer. 2009;9:271
references: 19656391
references: Cell Metab. 2010 Jun 9;11(6):479-92
references: 20519120
references: Vet Pathol. 2002 Jan;39(1):74-83
references: 12102221