title: Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture.
abstract: Rapid growth of shrimp farming industry is affected by the recurrence of diverse diseases, among which vibriosis is predominant. Eco-friendly disease management strategy by the application of antagonistic probiotics is widely accepted. In the present study, culture conditions of antagonistic probiotics, Pseudomonas MCCB 102 and 103, were optimized to enhance their biomass production and antagonistic activity against the shrimp pathogen V. harveyi MCCB 111. Primarily, one-dimensional screening was carried out to fix the optimum range of sodium chloride concentration, pH and temperature. The second step optimization was done using a full-factorial central composite design of response surface methodology. As per the model, 12.9 g/L sodium chloride and pH 6.5 for Pseudomonas MCCB 102, and 5 g/L sodium chloride and pH 7 for Pseudomonas MCCB 103 were found to be ideal to maximize antagonistic activity. However, optimum temperature was the same (25 °C) for both isolates. Finally, the models were experimentally validated for enhanced biomass production and antagonistic activity. The optima for biomass and antagonistic activity were more or less the same, suggesting the possible influence of biomass on antagonistic activity.
authors: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, Cochin, 682016, Kerala, India.
authors: R
authors: R
authors: Preetha
authors: K K
authors: KK
authors: Vijayan
authors: N S
authors: NS
authors: Jayapraksh
authors: S V
authors: SV
authors: Alavandi
authors: T C
authors: TC
authors: Santiago
authors: I S Bright
authors: IS
authors: Singh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000898:Antibiosis; D017756:Aquaculture / Q000379:methods; D001431:Bacteriological Techniques; D018533:Biomass; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010619:Phenazines / Q000737:chemistry / Q000494:pharmacology; D019936:Probiotics; D011549:Pseudomonas / Q000502:physiology*; D013696:Temperature; D014733:Vibrio / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000517:prevention & control
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D010619:Phenazines; C000598831:phenazine
references: Dis Aquat Organ. 2005 Dec 30;68(1):39-45
references: 16465832
references: Appl Environ Microbiol. 1997 Mar;63(3):881-7
references: 16535555
references: J Bacteriol. 1990 Feb;172(2):884-900
references: 2153661
references: Appl Environ Microbiol. 2005 Apr;71(4):1745-53
references: 15811997
references: Antimicrob Agents Chemother. 1981 Dec;20(6):814-20
references: 6798928
references: J Clin Pathol. 1999 May;52(5):385-7
references: 10560362
references: World J Microbiol Biotechnol. 2013 Feb;29(2):257-65
references: 23161451
references: Annu Rev Phytopathol. 2006;44:417-45
references: 16719720
references: Lett Appl Microbiol. 2002;34(2):114-8
references: 11849506
references: Appl Environ Microbiol. 1999 Mar;65(3):969-73
references: 10049849
references: J Biosci Bioeng. 2000;90(5):473-83
references: 16232896
references: J Appl Microbiol. 2007 Apr;102(4):1043-51
references: 17381748
references: J Appl Microbiol. 2005;98(1):145-54
references: 15610427
references: Appl Environ Microbiol. 2004 Jul;70(7):4004-11
references: 15240276