title: Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid.
abstract: The development of boronic probes enabled reliable detection and quantitative analysis of hydrogen peroxide and peroxynitrite. The major product, in which boronate moiety of the probe is replaced by the hydroxyl group, is however common for both oxidants. Here, we describe how ortho-isomer of mitochondria-targeted phenylboronic acid can be used to detect and differentiate peroxynitrite-dependent and peroxynitrite-independent probe oxidation. This method highlights the detection and quantification of both the major, phenolic product and the minor, peroxynitrite-specific nitrated product of probe oxidation.
authors: Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA, jzielonk@mcw.edu.
authors: Jacek
authors: J
authors: Zielonka
authors: Adam
authors: A
authors: Sikora
authors: Jan
authors: J
authors: Adamus
authors: B
authors: B
authors: Kalyanaraman
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D030421:Peroxynitrous Acid; D006861:Hydrogen Peroxide
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