title: The action of pre-exercise low-level laser therapy (LLLT) on the expression of IL-6 and TNF-α proteins and on the functional fitness of elderly rats subjected to aerobic training.
abstract: The aim of the present study was to determine whether low-level laser therapy (LLLT), when used in conjunction with aerobic training, interferes with the expression of inflammatory markers IL-6 and TNF-α, thereby influencing the performance of old rats participating in swimming. A total of 30 Wistar rats (Rattus norvegicus albinus) were used for this study: 24 aged rats, and 6 young rats. The older animals were randomly divided into four groups designated as follows: aged-control, aged-exercise, aged-LLLT, aged-LLLT/exercise group, and young-control animals. Aerobic capacity (VO2max) was analyzed before and after training period. The aged-exercise and aged-LLLT/exercise groups were trained for 6 weeks. LLLT laser was applied before each training session with 808 nm and 4 J of energy to the indicated groups throughout training. The rats were euthanized, and muscle tissue and serum were collected for muscle cross-sectional area and IL-6 and TNF-α protein analysis. In VO2 showed statistical difference between young- and aged-control groups (used as baseline) (p < 0.05). The same difference can be observed in the young control group compared with all intervention groups (exercise, LLLT and LLLT + exercise). In comparison with the aged-control group, a difference was observed only for comparison with the exercise group (p < 0.05), and exercise associated with LLLT group (p < 0.001). Levels of IL-6 and TNF-α for the aged-exercise and the aged-LLLT/exercise groups were significantly decreased compared to the aged-control group (p < 0.05). Analysis of the transverse section of the gastrocnemius muscle showed a significant difference between the aged-exercise and aged-LLLT/exercise groups (p < 0.001). These results suggest that laser therapy in conjunction with aerobic training may provide a therapeutic approach for reducing the inflammatory markers (IL-6 and TNF-α), however, LLLT without exercise was not able to improve physical performance of aged rats.
authors: Postgraduate Program in Biophotonics Applied Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.
authors: Eliane Martins
authors: EM
authors: Amadio
authors: Andrey Jorge
authors: AJ
authors: Serra
authors: Simone A
authors: SA
authors: Guaraldo
authors: José Antônio
authors: JA
authors: Silva
authors: Ednei Luis
authors: EL
authors: Antônio
authors: Flávio
authors: F
authors: Silva
authors: Leslie Andrews
authors: LA
authors: Portes
authors: Paulo José Ferreira
authors: PJ
authors: Tucci
authors: Ernesto Cesar Pinto
authors: EC
authors: Leal-Junior
authors: Paulo de Tarso Camillo
authors: Pde T
authors: de Carvalho
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000528:radiation effects; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D028022:Low-Level Light Therapy; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism; D009929:Organ Size; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
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