title: Two-temperature formalin fixation preserves activation states efficiently.
abstract: Modern pathology is built around the principle of preserving tissues such that the in vivo molecular status is maintained at levels representative of the disease state. Tissues are immersed in a solution of fixative which slowly inactivates biological activities, thus preserving the sample. Further processing ultimately allows the tissue to be embedded into wax for thin sectioning and staining for interpretation microscopically. Every year, around 7 billion tissue samples are submitted for processing in the United States alone. With this huge workload, histology laboratories are looking for faster methods of performing fixation, which currently require from several hours to days to complete. Ideally, this procedure could be standardized and would be quicker with better preservation over a wide range of biologically relevant molecules.
authors: , 1910 East Innovation PARK Drive, Oro Valley, AZ, 85755, USA, David.Chafin@ventana.roche.com.
authors: David
authors: D
authors: Chafin
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D004789:Enzyme Activation; D005404:Fixatives / Q000737:chemistry* / Q000494:pharmacology; D005557:Formaldehyde / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D010766:Phosphorylation; D057285:Precision Medicine; D011494:Protein Kinases / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D013696:Temperature; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D005404:Fixatives; D005557:Formaldehyde; D011494:Protein Kinases