title: Iron-induced necrotic brain cell death in rats with different aerobic capacity.
abstract: Brain iron overload has a key role in brain injury after intracerebral hemorrhage (ICH). Our recent study demonstrated that ICH-induced brain injury was greater in low capacity runner (LCR) than in high capacity runner (HCR) rats. The present study examines whether iron-induced brain injury differs between LCRs and HCRs. Adult male LCR and HCR rats had an intracaudate injection of iron or saline. Rats were euthanized at 2 and at 24 h after T2 magnetic resonance imaging, and the brains were used for immunostaining and Western blotting. LCRs had more hemispheric swelling, T2 lesion volumes, blood-brain barrier disruption, and neuronal death at 24 h after iron injection (p < 0.05). Many propidium iodide (PI)-positive cells, indicative of necrotic cell death, were observed in the ipsilateral basal ganglia of both HCRs and LCRs at 2 h after iron injection. PI fluorescence intensity was higher in LCRs than in HCRs. In addition, membrane attack complex (MAC) expression was increased at 2 h after iron injection and was higher in LCRs than in HCRs. The PI-positive cells co-localized with MAC-positive cells in the ipsilateral basal ganglia. Iron induces more severe necrotic brain cell death, brain swelling, and blood-brain barrier disruption in LCR rats, which may be related with complement activation and MAC formation.
authors: Department of Neurosurgery, University of Michigan, R5018 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200, USA.
authors: Mingzhe
authors: M
authors: Zheng
authors: Hanjian
authors: H
authors: Du
authors: Wei
authors: W
authors: Ni
authors: Lauren G
authors: LG
authors: Koch
authors: Steven L
authors: SL
authors: Britton
authors: Richard F
authors: RF
authors: Keep
authors: Guohua
authors: G
authors: Xi
authors: Ya
authors: Y
authors: Hua
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000473:pathology; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D007501:Iron / Q000633:toxicity*; D008297:Male; D009336:Necrosis; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D010805:Physical Conditioning, Animal; D051381:Rats
chemical_list: D015938:Complement Membrane Attack Complex; D007501:Iron
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