title: Hippocampal neuronal maturation triggers post-synaptic clustering of brain temperature-sensor TRPV4.
abstract: Compartmentalization of neuronal function is achieved via specifically localized clustering of ion channels in discrete subcellular membrane domains. Transient receptor potential (TRP) channels exhibit highly variable cellular and subcellular patterns of expression. We previously revealed that the thermo-sensor TRPV4 (activated above 34 °C) is gated by physiological brain temperatures in hippocampal neurons and thereby controls their excitability. Here, we examined synaptic clustering of TRPV4 in developing hippocampal neurons. We found that TRPV4 accumulated in the soma of immature hippocampal neurons, and did not localize to post-synaptic locations although PSD-95-labeled post-synaptic structures were evident. During the maturation of neurons, TRPV4 was targeted to dendrites and also clustered at post-synaptic locations. Taken together, we reveal that TRPV4 localizes to post-synaptic sites and the post-synaptic targeting is strictly regulated in a neuronal maturation-dependent manner.
authors: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. Electronic address: shibasaki@gunma-u.ac.jp.
authors: Koji
authors: K
authors: Shibasaki
authors: Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki 444-8787, Japan; Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Department of Physiological Sciences, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan.
authors: Makoto
authors: M
authors: Tominaga
authors: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
authors: Yasuki
authors: Y
authors: Ishizaki
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C486576:Trpv4 protein, mouse
keywords: Cerebellum; Development; Hippocampus; PSD-95; Synapse; TRPV4