title: Efficacy of different anaesthetics for pikeperch (Sander lucioperca L.) in relation to water temperature.
abstract: OBJECTIVE
  The objectives of the study were to compare the different doses of clove oil, Propiscin, and tricaine methane sulphonate (MS 222) in relation to water temperature in pikeperch aquaculture.
  
  
  METHODS
  For assessment of this experiment 168 fish (10.77 ± 0.59 cm total body length and 7.88 ± 1.74 g body weight) were used. Three different anaesthetic treatments (Propiscin, clove oil and MS 222) were used. Three doses of each anaesthetic treatment (Propiscin: 0.5; 1; 1.5 ml x L(-1), clove oil: 15; 30; 60 mg x L(-1), MS 222: 50; 100; 150 mg x L(-1)) were compared at three different temperatures 9.5; 15.5 and 23 degrees C.
  
  
  RESULTS
  In comparison of these doses of anaesthetic in different temperature, the significantly shortest time to attain phase A7 (total complete anaesthesia) was observed for Propiscin (1.5 ml L(-1)) 0:31 ± 0:04 min (23 degrees C) to 0:33 ± 0:25 min (9.5 degrees C) compared to MS 222 (150 mg x L(-1)) 1:04 ± 0:21 min (23 degrees C) to 1:54 ± 0:32 min (9.5 degrees C) and clove oil (60 mg x L(-1)) 1:05 ± 0:17 min (23 degrees C) to 3:05 ± 0:31 min (9.5 degrees C). On the other hand, the longest time of anaesthesia recovery was attained using Propiscin (1.5 ml x L(-1)) 10:35 ± 1:40 min (23 degrees C) to 32:30 ± 1:10 min (9.5 degrees C) compared to clove oil (60 mg x L(-1)) 2:39 ± 0:50 min (23 degrees C) to 9:36 ± 2:34 min (60 mg x L(-1), 9.5 degrees C) and MS 222 (150 mg x L(-1)) 2:26 ± 1:27 min (23 degrees C) to 4:59 ± 0:39 min (9.5 degrees C).
  
  
  CONCLUSIONS
  The results from this study showed that the optimal and sufficient doses in all tested temperatures for pikeperch are 30 mg x L(-1) of clove oil, 100 mg x L(-1) of MS 222 and 0.5 ml x L(-1) of Propiscin.
authors: Jiri
authors: J
authors: Kristan
authors: Alzbeta
authors: A
authors: Stara
authors: Miroslav
authors: M
authors: Polgesek
authors: Alexandru
authors: A
authors: Drasovean
authors: Jitka
authors: J
authors: Kolarova
authors: Josef
authors: J
authors: Priborsky
authors: Miroslav
authors: M
authors: Blecha
authors: Petr
authors: P
authors: Svacina
authors: Tomas
authors: T
authors: Policar
authors: Josef
authors: J
authors: Velisek
mesh_terms: D062365:Aminobenzoates / Q000494:pharmacology*; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D045783:Clove Oil / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005045:Etomidate / Q000494:pharmacology*; D010471:Perches; D013696:Temperature; D014867:Water
chemical_list: D062365:Aminobenzoates; D000777:Anesthetics; D045783:Clove Oil; C003636:tricaine; D014867:Water; D005045:Etomidate