title: Reference gene selection for cross-species and cross-ploidy level comparisons in Chrysanthemum spp.
abstract: The establishment of a (set of) stably expressed reference gene(s) is required to normalize transcription data. Polyploidy is very common in the plant kingdom, but it is not necessarily the case that a reference gene which works well at the diploid level will also work well at the polyploid level. Here, ten candidate reference genes are compared in the context of gene transcription in the genus Chrysanthemum. The robustness of some, but not all, of these was shown to be high across ploidy levels. MTP (metalloprotease) and ACTIN (actin) were the most stable in diploid and tetraploid C. nankingense, while PSAA (photosynthesis-related plastid gene representing photosystem I) and EF-1α (elongation factor-1α) were the most stable in tetraploid and hexaploid C. zawadskii. EF-1α and PGK (phosphoglycerate kinase) was the best combination for the complete set of four taxa. These results suggest that when making cross-species comparison of transcript abundance involving different ploidy levels, care needs to be taken in the selection of reference gene(s).
authors: 1] College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China [2] Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology &Equipment, Nanjing 210095, China.
authors: Haibin
authors: H
authors: Wang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Sumei
authors: S
authors: Chen
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jiafu
authors: J
authors: Jiang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors: Fei
authors: F
authors: Zhang
authors: 1] College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China [2] Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology &Equipment, Nanjing 210095, China.
authors: Fadi
authors: F
authors: Chen
mesh_terms: D031202:Chrysanthemum / Q000235:genetics*; D003433:Crosses, Genetic; D004171:Diploidy; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D011003:Ploidies; D011123:Polyploidy; D020871:RNA Stability; D051135:Rad51 Recombinase / Q000235:genetics; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D051135:Rad51 Recombinase
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