title: Effects of selenium-chitosan on blood selenium concentration, antioxidation status, and cellular and humoral immunity in mice.
abstract: One hundred and eighty Kunming mice were allotted to three groups in a randomized complete block design, including two treatments and one control. Mice in group 1 were fed a basal diet as control, while mice in groups 2 and 3 were fed the basal diet supplemented with 0.2 mg/kg selenium as sodium selenite (SS) or selenium-chitosan (SC), respectively. On day 28 of the experiment, blood selenium concentration, glutathione peroxidase (GPx) activity, plasma superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and Con A-induced splenocyte proliferation were determined, and plasma interleukin-2 (IL-2) and interferon-γ (IFN-γ) concentrations, splenic plaque-forming cell (PFC) responses, serum hemolysis level (HC50), and delayed-type hypersensitivity (DTH) responses were determined on day 15 of the experiment. The results showed that blood selenium concentration, GPx activity, splenic PFC response, and plasma IL-2 and IFN-γ concentrations in SC group were higher than those in the control and SS groups (P < 0.01 or P < 0.05), respectively. Plasma SOD activity, Serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were higher than those in control (P < 0.01 or P < 0.05). Plasma SOD activity, serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were also higher than those in SS group, while there was no significant difference between SC and SS groups (P > 0.05). Plasma MDA content in SC group was lower than those in the control and SS groups (P < 0.01 or P < 0.05). It is concluded that SC supplement can increase blood selenium concentration, antioxidation status, and cellular and humoral immunity, and SC has better biological activity than SS in mice.
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Shunyi
authors: S
authors: Qin
authors: Baoxia
authors: B
authors: Huang
authors: Jifei
authors: J
authors: Ma
authors: Xin
authors: X
authors: Wang
authors: Jianbing
authors: J
authors: Zhang
authors: Liuan
authors: L
authors: Li
authors: Fu
authors: F
authors: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006461:Hemolysis; D007111:Immunity, Cellular / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D007376:Interleukin-2 / Q000097:blood / Q000378:metabolism; D008297:Male; D051379:Mice; D011897:Random Allocation; D012643:Selenium / Q000097:blood* / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry*; D012756:Sheep; D018038:Sodium Selenite / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D013482:Superoxide Dismutase
chemical_list: D000975:Antioxidants; D007376:Interleukin-2; D018036:Selenium Compounds; D007371:Interferon-gamma; D048271:Chitosan; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D018038:Sodium Selenite