title: Dibenzoylmethane, hydroxydibenzoylmethane and hydroxymethyldibenzoylmethane inhibit phorbol-12-myristate 13-acetate‑induced breast carcinoma cell invasion.
abstract: Dibenzoylmethane (DB), a minor constituent of the root extract of licorice, belongs to the flavonoid family. Hydroxydibenzoylmethane (HDB) and hydroxymethyldibenzoylmethane (HMDB) have an identical structure to DB, but also possess a hydroxyl group and a hydroxyl and methyl group bonded to aromatic rings, respectively. They inhibit cellular proliferation and induce apoptosis in a variety of types of cancer cell, however, the antimetastatic effects of DB, HDB and HMDB on human breast carcinoma cells remain to be elucidated. The present study aimed to clarify the molecular mechanisms underlying the effects of DB and its analogues on phorbol‑12‑myristate 13‑acetate (PMA)‑induced MCF‑7 cell metastasis. The results revealed that DB, HDB and HMDB inhibited cell migration and invasion. In addition, PMA‑mediated MCF‑7 cell invasion was inhibited by DB, HDB and HMDB by inhibiting the expression of matrix metalloproteinase (MMP)‑9. Rottlerin, a protein kinase C (PKC)δ inhibitor and LY294002, a phosphatidylinositide 3‑kinase (PI3K) inhibitor, reduced the PMA‑mediated expression of MMP‑9 and cell invasion. Furthermore, DB, HDB and HMDB prevented the activation of PKCδ and PI3K by inhibiting their phosphorylation. The present study was the first, to the best of our knowledge, to demonstrate the antimetastatic potential of DB, HDB and HDMB, which decreased cancer cell invasion through the PI3K/PKCδ‑mediated MMP‑9 pathway.
authors: Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan, R.O.C.
authors: Ya-Fan
authors: YF
authors: Liao
authors: Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan, R.O.C.
authors: Yew-Min
authors: YM
authors: Tzeng
authors: Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, R.O.C.
authors: Hui-Chih
authors: HC
authors: Hung
authors: Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C.
authors: Guang-Yaw
authors: GY
authors: Liu
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D001578:Benzopyrans / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D002867:Chromones / Q000494:pharmacology; D005260:Female; D006801:Humans; D007659:Ketones / Q000494:pharmacology*; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation / Q000187:drug effects; D011407:Propane / Q000031:analogs & derivatives* / Q000494:pharmacology; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
chemical_list: D000098:Acetophenones; D001578:Benzopyrans; D047188:Chalcones; D002867:Chromones; D007659:Ketones; D020780:Matrix Metalloproteinase 9; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011407:Propane; D051745:Protein Kinase C-delta; D013755:Tetradecanoylphorbol Acetate; C506624:1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione; C476492:hydroxydibenzoylmethane; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C061481:dibenzoylmethane; C085746:rottlerin