title: Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer.
abstract: BACKGROUND
  Lipoprotein lipase (LPL) plays a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of serum LPL is useful for diagnosing lipid disorders, but there is no rapid method of measuring LPL for clinical use.
  
  
  METHODS
  We developed a rapid and sensitive latex particle-enhanced turbidimetric immunoassay (LTIA) serum LPL using latex bead-immobilized anti-LPL monoclonal antibodies. The assay was performed on a Hitachi 7700 P analyzer and evaluated for its validity as a method of quantitating the serum LPL concentration in parallel with ELISA.
  
  
  RESULTS
  Dilution tests using LTIA produced a calibration curve from 0.5 to 800ng/ml. Within-run CV was obtained in the range of 2.2-5.5%. No interference was observed in the testing of specimens containing potentially interfering substances such as bilirubin-F and C, hemoglobin, triglycerides and rheumatoid factor. A strong correlation between LTIA and ELISA was confirmed (n=40, r=0.967, y=0.99x-1.86). The normal range of LPL in pre-heparin serum was 50-77ng/ml and in post-heparin plasma 354-410ng/ml, respectively.
  
  
  CONCLUSIONS
  The LTIA assay is applicable in quantitating the concentration of LPL in both pre-heparin serum and post-heparin plasma. This assay is more convenient and faster than ELISA and highly suitable for clinical routine analysis.
authors: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan; Gunma University Graduate School of Health Sciences, Maebashi, Gunma, Japan.
authors: Tetsuo
authors: T
authors: Machida
authors: Immuno-Biological Laboratories, Fujioka, Gunma, Japan.
authors: Kazuya
authors: K
authors: Miyashita
authors: Fujikura-Kasei, Co., Ltd, Sano, Tochigi, Japan.
authors: Takuya
authors: T
authors: Sone
authors: Fujikura-Kasei, Co., Ltd, Sano, Tochigi, Japan.
authors: Sayori
authors: S
authors: Tanaka
authors: Gunma University Graduate School of Health Sciences, Maebashi, Gunma, Japan.
authors: Katsuyuki
authors: K
authors: Nakajima
authors: Department of Nutrition, School of Nursing and Nutrition, Tenshi College, Sapporo, Japan.
authors: Masayuki
authors: M
authors: Saito
authors: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, United States.
authors: Kimber
authors: K
authors: Stanhope
authors: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, United States.
authors: Peter
authors: P
authors: Havel
authors: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors: Hiroyuki
authors: H
authors: Sumino
authors: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. Electronic address: mmurakam@gunma-u.ac.jp.
authors: Masami
authors: M
authors: Murakami
mesh_terms: D001331:Automation; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007840:Latex / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008071:Lipoprotein Lipase / Q000097:blood*; D008297:Male; D009391:Nephelometry and Turbidimetry; D012016:Reference Values; D055815:Young Adult
chemical_list: D007840:Latex; D008071:Lipoprotein Lipase
keywords: Automated analyzer; ELlSA; Latex particle-enhanced turbidimetric immunoassay; Lipoprotein lipase (LPL); TG-rich lipoproteins