title: In vivo visualization and quantification of mitochondrial morphology in C. elegans.
abstract: Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.
authors: Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences (SILS), Faculty of Science (FNWI), University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.
authors: Reuben L
authors: RL
authors: Smith
authors: Winnok H
authors: WH
authors: De Vos
authors: Richard
authors: R
authors: de Boer
authors: Erik M M
authors: EM
authors: Manders
authors: Hans
authors: H
authors: van der Spek
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*