title: Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica.
abstract: Bordetella bronchiseptica is a pathogenic bacterium causing respiratory infections in a broad range of mammals. Recently, we determined the whole genome sequence of B. bronchiseptica S798 strain isolated from a pig infected with atrophic rhinitis and found four single-nucleotide polymorphisms (SNPs) at positions -129, -72, +22, and +38 in the region upstream of dnt encoding dermonecrotic toxin (DNT), when compared with a rabbit isolate, RB50. DNT is known to be involved in turbinate atrophy observed in atrophic rhinitis. Immunoblotting, quantitative real-time PCR, and β-galactosidase reporter assay revealed that these SNPs resulted in the increased promoter activity of dnt and conferred the increased ability to produce DNT on the bacteria. Similar or identical SNPs were also found in other pig isolates kept in our laboratory, all of which produce a larger amount of DNT than RB50. Our analysis revealed that substitution of at least two of the four bases, at positions -72 and +22, influenced the promoter activity for dnt. These results imply that these SNPs are involved in the pathogenicity of bordetellae specific to pig diseases.
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Keisuke
authors: K
authors: Okada
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Hiroyuki
authors: H
authors: Abe
authors: Experimental Animal Division, RIKEN BioResource Center, Ibaraki, Japan.
authors: Fumio
authors: F
authors: Ike
authors: Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan; Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors: Yoshitoshi
authors: Y
authors: Ogura
authors: Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan; Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors: Tetsuya
authors: T
authors: Hayashi
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Aya
authors: A
authors: Fukui-Miyazaki
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Keiji
authors: K
authors: Nakamura
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Naoaki
authors: N
authors: Shinzawa
authors: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Yasuhiko
authors: Y
authors: Horiguchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D013045:Species Specificity; D011503:Transglutaminases / Q000235:genetics*; D010566:Virulence Factors, Bordetella / Q000235:genetics*
chemical_list: D010566:Virulence Factors, Bordetella; C060969:dermonecrotic toxin, Bordetella; D011503:Transglutaminases
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