title: Spinal NF-κB and chemokine ligand 5 expression during spinal glial cell activation in a neuropathic pain model.
abstract: BACKGROUND
  The NF-κB pathway and chemokine (C-C motif) ligand 5 (CCL5) are involved in pain modulation; however, the precise mechanisms of their interactions in chronic neuropathic pain have yet to be established.
  
  
  METHODS
  The present study examined the roles of spinal NF-κB and CCL5 in a neuropathic pain model after chronic constriction injury (CCI) surgery. CCI-induced pain facilitation was evaluated using the Plantar and von Frey tests. The changes in NF-κB and CCL5 expression were analyzed by immunohistochemistry and Western blot analyses.
  
  
  RESULTS
  Spinal NF-κB and CCL5 expression increased after CCI surgery. Repeated intrathecal infusions of pyrrolidine dithiocarbamate (PDTC, a NF-κB inhibitor) decreased CCL5 expression, inhibited the activation of microglia and astrocytes, and attenuated CCI-induced allodynia and hyperalgesia. Intrathecal injection of a CCL5-neutralizing antibody attenuated CCI-induced pain facilitation and also suppressed spinal glial cell activation after CCI surgery. However, the CCL5-neutralizing antibody did not affect NF-κB expression. Furthermore, selective glial inhibitors, minocycline and fluorocitrate, attenuated the hyperalgesia induced by intrathecal CCL5.
  
  
  CONCLUSIONS
  The inhibition of spinal CCL5 expression may provide a new method to prevent and treat nerve injury-induced neuropathic pain.
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Qin
authors: Q
authors: Yin
authors: Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China; Affiliated Heping Hospital of Changzhi Medical College, Changzhi, China.
authors: Qin
authors: Q
authors: Fan
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Yu
authors: Y
authors: Zhao
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Ming-Yue
authors: MY
authors: Cheng
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: He
authors: H
authors: Liu
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China.
authors: Jing
authors: J
authors: Li
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China.
authors: Fei-Fei
authors: FF
authors: Lu
authors: Affiliated Heping Hospital of Changzhi Medical College, Changzhi, China.
authors: Jin-Tai
authors: JT
authors: Jia
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China; Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Wei
authors: W
authors: Cheng
authors: Xuzhou Medical College, Xuzhou, China; Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, China.
authors: Chang-Dong
authors: CD
authors: Yan
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D018946:Chemokine CCL5 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006930:Hyperalgesia / Q000378:metabolism / Q000503:physiopathology; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D009437:Neuralgia / Q000235:genetics* / Q000503:physiopathology; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism / Q000503:physiopathology
chemical_list: D018946:Chemokine CCL5; D016328:NF-kappa B
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