title: Ppp2ca knockout in mice spermatogenesis.
abstract: Protein phosphatase 2A (PP2A) is a ubiquitous serine/threonine phosphatase involved in meiosis, mitosis, sperm capacitation, and apoptosis. Abberant activity of PP2A has been associated with a number of diseases. The homolog PPP2CA and PPP2CB can each function as the phosphatase catalytic subunit generally referred to as PP2AC. We generated a Ppp2ca conditional knockout (CKO) in C57BL/6J mice. Exon 2 of Ppp2ca was knocked out in a spatial or temporal-specific manner in primordial germ cells at E12.5. This Ppp2ca-null mutation caused infertility in male C57BL/6J mice. These CKO mice provide a powerful tool to study the mechanisms of Ppp2ca in development and disease.
authors: Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors: Xiaoyun
authors: X
authors: Pan
authors: Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors: Xia
authors: X
authors: Chen
authors: Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors: Xin
authors: X
authors: Tong
authors: Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors: Chao
authors: C
authors: Tang
authors: Key Laboratory of National Reproductive MedicineDepartment of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China jianminlilab@163.com.
authors: Jianmin
authors: J
authors: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D007248:Infertility, Male / Q000209:etiology*; D019426:Integrases / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054648:Protein Phosphatase 2 / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; C045073:Cre recombinase; D019426:Integrases; C578496:PPP2CA protein, mouse; D054648:Protein Phosphatase 2