title: Overexpression of copper/zinc superoxide dismutase from mangrove Kandelia candel in tobacco enhances salinity tolerance by the reduction of reactive oxygen species in chloroplast.
abstract: Na(+) uptake and transport in Kandelia candel and antioxidative defense were investigated under rising NaCl stress from 100 to 300 mM. Salinized K. candel roots had a net Na(+) efflux with a declined flux rate during an extended NaCl exposure. Na(+) buildup in leaves enhanced H2O2 levels, superoxide dismutase (SOD) activity, and increased transcription of CSD gene encoding a Cu/Zn SOD. Sequence and subcellular localization analyses have revealed that KcCSD is a typical Cu/Zn SOD in chloroplast. The transgenic tobacco experimental system was used as a functional genetics model to test the effect of KcCSD on salinity tolerance. KcCSD-transgenic lines were more Na(+) tolerant than wild-type (WT) tobacco in terms of lipid peroxidation, root growth, and survival rate. In the latter, 100 mM NaCl led to a remarkable reduction in chlorophyll content and a/b ratio, decreased maximal chlorophyll a fluorescence, and photochemical efficiency of photosystem II. NaCl stress in WT resulted from H2O2 burst in chloroplast. Na(+) injury to chloroplast was less pronounced in KcCSD-transgenic plants due to upregulated antioxidant defense. KcCSD-transgenic tobacco enhanced SOD activity by an increment in SOD isoenzymes under 100 mM NaCl stress from 24 h to 7 day. Catalase activity rose in KcCSD overexpressing tobacco plants. KcCSD-transgenic plants better scavenged NaCl-elicited reactive oxygen species (ROS) compared to WT ones. In conclusion, K. candel effectively excluded Na(+) in roots during a short exposure; and increased CSD expression to reduce ROS in chloroplast in a long-term and high saline environment.
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Xiaoshu
authors: X
authors: Jing
authors: Department of Bio-Instruments, National Engineering Research Center for Information Technology in Agriculture Beijing, China.
authors: Peichen
authors: P
authors: Hou
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Yanjun
authors: Y
authors: Lu
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Shurong
authors: S
authors: Deng
authors: Department of Biology, College of Life Science, Hainan Normal University Haikou, China.
authors: Niya
authors: N
authors: Li
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Rui
authors: R
authors: Zhao
authors: Department of Plant Science, College of Life Science, Jiangsu Normal University Xuzhou, China.
authors: Jian
authors: J
authors: Sun
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Yang
authors: Y
authors: Wang
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Yansha
authors: Y
authors: Han
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Tao
authors: T
authors: Lang
authors: Department of Crop Science, College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University Hangzhou, China.
authors: Mingquan
authors: M
authors: Ding
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Xin
authors: X
authors: Shen
authors: Department of Plant Science, College of Biological Sciences and Technology, Beijing Forestry University Beijing, China.
authors: Shaoliang
authors: S
authors: Chen
keywords: Kandelia candel; Na+ flux; catalase; hydrogen peroxide; salt; superoxide anion; superoxide dismutase
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