title: Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody.
abstract: CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2gc-null) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.
authors: Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine.
authors: Shino
authors: S
authors: Ohshima
authors: Syuuya
authors: S
authors: Mori
authors: Atsuko
authors: A
authors: Shigenari
authors: Asuka
authors: A
authors: Miyamoto
authors: Masaki
authors: M
authors: Takasu
authors: Noriaki
authors: N
authors: Imaeda
authors: Satoshi
authors: S
authors: Nunomura
authors: Yoshimichi
authors: Y
authors: Okayama
authors: Masafumi
authors: M
authors: Tanaka
authors: Hitoshi
authors: H
authors: Kitagawa
authors: Jerzy K
authors: JK
authors: Kulski
authors: Hidetoshi
authors: H
authors: Inoko
authors: Asako
authors: A
authors: Ando
authors: Yoshie
authors: Y
authors: Kametani
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000502:physiology*; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology; D013045:Species Specificity; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D019009:Proto-Oncogene Proteins c-kit