title: Analysis of mitochondrial DNA and RNA integrity by a real-time qPCR-based method.
abstract: This chapter describes the use of real-time qPCR to analyze the integrity of mitochondrial nucleic acids quantitatively. The method has low material requirement, is low cost, and can detect modifications with high resolution. The method is specifically designed for mitochondrial RNA and DNA, but can be easily transferred to other high-copy number cases. This procedure describes analyses of brain nucleic acids, but other tissues or cells can be analyzed similarly.
authors: Department of Medical Biochemistry, Institute of Clinical Medicine, Rikshospitalet, University of Oslo, Sognsvannsveien 20, 0027, Oslo, Norway.
authors: Wei
authors: W
authors: Wang
authors: Ying
authors: Y
authors: Esbensen
authors: Katja
authors: K
authors: Scheffler
authors: Lars
authors: L
authors: Eide
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D009154:Mutation; D059645:Mutation Rate; D011786:Quality Control; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000077278:RNA, Mitochondrial; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards
chemical_list: D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012313:RNA