title: Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles.
abstract: The nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. Historically, averaged bulk analysis of viral particles has been the primary method to quantitatively investigate these parameters, though this neglects heterogeneity within populations. Here we analyse the properties of Junin virus particles using a sensitive flow virometry assay and further sort virions while conserving their infectiveness. This method allows us to characterize the relationship between infectivity, virus size and RNA content and to compare particles secreted by Vero cells with those from physiologically relevant human primary macrophages. Our study highlights significant differences in particle infectivity according to its nature, the type of producer cells and the lipid membrane composition at the budding site. Together, our results present the flow virometry assay as a powerful and versatile tool to define virus particle profiles.
authors: 1] Program in Cellular and Molecular Medicine, Boston Children's Hospital, 200 Longwood Avenue, Boston, Massachusetts 02115, USA [2] Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors: Raphaël
authors: R
authors: Gaudin
authors: 1] Program in Cellular and Molecular Medicine, Boston Children's Hospital, 200 Longwood Avenue, Boston, Massachusetts 02115, USA [2] Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors: Natasha S
authors: NS
authors: Barteneva
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D018051:Junin virus / Q000472:pathogenicity / Q000648:ultrastructure; D008264:Macrophages / Q000821:virology; D046529:Microscopy, Electron, Transmission; D009000:Monocytes / Q000821:virology; D014709:Vero Cells; D014771:Virion / Q000472:pathogenicity* / Q000648:ultrastructure
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