title: The Associations of Novel Vitamin D3 Metabolic Gene CYP27A1 Polymorphism, Adiponectin/Leptin Ratio, and Metabolic Syndrome in Middle-Aged Taiwanese Males.
abstract: Metabolic syndrome (MetS) confers increased risks of cardiovascular disease (CVD). Both vitamin D3 and adipocytokines (especially adiponectin and leptin) have a great impact on CVD and MetS. In vitamin D3 metabolism, the vitamin D3 25-hydroxylase (CYP27A1) and 25-hydroxyvitamin D3 1-alpha-hydroxylase (CYP27B1) are two key enzymes. This study aimed to examine the influence of vitamin D3 CYP27 single nucleotide polymorphisms (SNPs) on adipocytokines and MetS. Cross-sectional data and DNA samples were collected from male volunteers (n = 649, age: 55.7 ± 4.7 years). Two tagging SNPs, CYP27A1 rs4674344 and CYP27B1 rs10877012, were selected from the HapMap project. MetS was significantly associated with the CYP27A1 rs4674344 SNP (P = 0.04) and the ratio of adiponectin/leptin (A/L ratio) was most correlated to the CYP27A1 rs4674344 SNP, appearing to be significantly lower in T-carriers than in AA subjects (3.7 ± 4.0 versus 5.1 ± 6.0, P = 0.001) and significantly negatively associated after adjustment. For each MetS component associated with the CYP27A1 rs4674344 SNP, the A/L ratios were significantly negative in preclinical stage (condition not meeting the individual criteria), except the blood pressure. In conclusion, CYP27A1 rs4674344 SNP, A/L ratio, and MetS are significantly associated and T-carriers might have a higher risk of developing MetS due to low A/L ratios in the preclinical stage.
authors: Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Kai-Hung
authors: 0000-0003-1066-9499
authors: KH
authors: Cheng
authors: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
authors: Edward
authors: 0000-0002-8272-6707
authors: E
authors: Hsi
authors: Department of Urology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Department of Urology, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Pingtung Hospital, Department of Health, Executive Yuan, Pingtung 90054, Taiwan.
authors: Chia-Chu
authors: CC
authors: Liu
authors: Department of Urology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Department of Urology, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Chun-Nung
authors: CN
authors: Huang
authors: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Department of Urology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Department of Urology, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Yung-Chin
authors: YC
authors: Lee
authors: Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Chih-Sheng
authors: CS
authors: Chu
authors: Department of Pharmacy, China Medical University, Taichung 40402, Taiwan.
authors: Bo-Ying
authors: 0000-0001-5510-6513
authors: BY
authors: Bao
authors: Department of Physical Therapy, Tzu Chi University, Hualien 97004, Taiwan.
authors: Chu-Fen
authors: CF
authors: Chang
authors: Department of Urology, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Department of Urology, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Shu-Pin
authors: 0000-0003-2968-6125
authors: SP
authors: Huang
authors: Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Po-Lin
authors: PL
authors: Kuo
authors: Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan ; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors: Wen-Ter
authors: WT
authors: Lai
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