title: Preserving GABAergic interneurons in acute brain slices of mice using the N-methyl-D-glucamine-based artificial cerebrospinal fluid method.
abstract: Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recording in various ex vivo methods. However, it is difficult to maintain the activity and morphology of neurons in slices from mice of >30 days old. Here we evaluated the N-methyl-D-glucamine (NMDG)-based artificial cerebrospinal fluid (aCSF) method for the preservation of interneurons in slices from mice of up to ∼6 months old and discussed the steps that may affect their quality during slicing. We found that the NMDG-aCSF method rescued more cells than sucrose-aCSF and successfully preserved different types of interneurons including parvalbumin- and somatostatin-positive interneurons. In addition, both the chemical and electrical synaptic signaling of interneurons were maintained. These results demonstrate that the NMDG-aCSF method is suitable for the preservation of interneurons, especially in studies of gap junctions.
authors: Institute of Neuroscience, Key Laboratory of Medical Neurobiology of the Ministry of Health, Zhejiang Province Key Laboratory of Neurobiology, School of Medicine, Zhejiang University, Hangzhou, 310058, China.
authors: Geng
authors: G
authors: Pan
authors: Yue
authors: Y
authors: Li
authors: Hong-Yan
authors: HY
authors: Geng
authors: Jian-Ming
authors: JM
authors: Yang
authors: Ke-Xin
authors: KX
authors: Li
authors: Xiao-Ming
authors: XM
authors: Li
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena / Q000187:drug effects / Q000235:genetics; D001921:Brain / Q000166:cytology*; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism; D004558:Electric Stimulation; D059330:GABAergic Neurons / Q000187:drug effects* / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D016202:N-Methylaspartate / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010320:Parvalbumins / Q000378:metabolism; D018408:Patch-Clamp Techniques; D013004:Somatostatin / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D010320:Parvalbumins; D013004:Somatostatin; D013395:Sucrose; D016202:N-Methylaspartate; D002766:Cholecystokinin; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
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