title: 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.
abstract: Our objective was to explore molecular markers and mechanism of BDE47 on spermatogenesis in mammals. Adult male SD rats were gavaged daily with corn oil containing 0, 0.001, 0.03, 1 or 20mg BDE47/kg bw for eight weeks. Testes morphology was analyzed using electron microscopy, TUNEL, immunohistochemistry and morphometry. Differential proteome profile and western blotting were applied to determine molecular markers and protein expression. GC1-spg cells (mouse spermatogonial cells) were used to verify mechanism of BDE47. Data showed BDE47 reduced tubular epithelial thickness, impaired mitochondrial function and induced apoptosis in early leptotene spermatocytes. Proteomic study identified 70 differential spots corresponding to 64 proteins. 20 proteins related to apoptosis, 15 located in mitochondria. Exposure of GC1-spg cells showed BDE47 induced apoptosis, impaired mitochondria and decreased Bcl-2 in cells. Data indicate that BDE47 disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats probably via mitochondrial pathway.
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China; Department of Human Anatomy and Neuroscience, Medical School, Southeast University, Nanjing, Jiangsu, China.
authors: Shaoping
authors: S
authors: Huang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Yiqiang
authors: Y
authors: Cui
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Xuejiang
authors: X
authors: Guo
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China. Electronic address: wanglei@njmu.edu.cn.
authors: Lei
authors: L
authors: Wang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Suying
authors: S
authors: Li
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Ying
authors: Y
authors: Lu
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Ye
authors: Y
authors: Bi
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Xiaoyan
authors: X
authors: Huang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Min
authors: M
authors: Lin
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Yankai
authors: Y
authors: Xia
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Shoulin
authors: S
authors: Wang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Xinru
authors: X
authors: Wang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China. Electronic address: zhouzm@njmu.edu.cn.
authors: Zuomin
authors: Z
authors: Zhou
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, China.
authors: Jiahao
authors: J
authors: Sha
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D005411:Flame Retardants / Q000633:toxicity*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics; D017207:Rats, Sprague-Dawley; D013090:Spermatocytes / Q000187:drug effects* / Q000648:ultrastructure; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism
chemical_list: D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: Apoptosis; Mitochondria; PBDEs; Spermatogenesis; Testis