title: Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens.
abstract: Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens.
authors: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors: Arunee
authors: A
authors: Thanasarasakulpong
authors: Pichayanut
authors: P
authors: Poolperm
authors: Pallop
authors: P
authors: Tankaew
authors: Takuo
authors: T
authors: Sawada
authors: Nattawooti
authors: N
authors: Sthitmatee
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D004768:Enterotoxins; D009838:Oligodeoxyribonucleotides; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary; D016979:Pasteurella multocida / Q000145:classification* / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins
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