title: Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.
authors: Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors: Shuping
authors: S
authors: Nie
authors: Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Baoyu
authors: B
authors: Tian
authors: Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA.
authors: Xiaowei
authors: X
authors: Wang
authors: bioMérieux, Inc., Hazelwood, Missouri, USA.
authors: David H
authors: DH
authors: Pincus
authors: bioMérieux SA, La Balme les Grottes, France.
authors: Martin
authors: M
authors: Welker
authors: Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors: Kathleen
authors: K
authors: Gilhuley
authors: Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China.
authors: Xuedong
authors: X
authors: Lu
authors: Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA Department of Reproductive Biology, Case Western Reserve University, Cleveland, Ohio, USA ywh2102@columbia.edu tangy@mskcc.org.
authors: Yiping W
authors: YW
authors: Han
authors: Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York, USA ywh2102@columbia.edu tangy@mskcc.org.
authors: Yi-Wei
authors: http://orcid.org/0000-0003-4888-6771
authors: YW
authors: Tang
mesh_terms: D015373:Bacterial Typing Techniques; D005674:Fusobacterium Infections / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000145:classification* / Q000235:genetics*; D006801:Humans; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
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