title: Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds.
abstract: Regenerative medicine, tissue engineering and biomedical research give hope to many patients who need bio-implants. Tissue engineering applications have already been developed based on bioreactors. Physiological ureter implants, however, do not still function sufficiently, as they represent tubular hollow structures with very specific cellular structures and alignments consisting of several cell types. The aim of this study was to a develop a new bioreactor system based on seamless, collagenous, tubular OPTIMAIX 3D prototype sponge as scaffold material for ex-vivo culturing of a tissue engineered ureter replacement for future urological applications. Particular emphasis was given to a great extent to mimic the physiological environment similar to the in vivo situation of a ureter. NIH-3T3 fibroblasts, C2C12, Urotsa and primary genitourinary tract cells were applied as co-cultures on the scaffold and the penetration of cells into the collagenous material was followed. By the end of this study, the bioreactor was functioning, physiological parameter as temperature and pH and the newly developed BIOREACTOR system is applicable to tubular scaffold materials with different lengths and diameters. The automatized incubation system worked reliably. The tubular OPTIMAIX 3D sponge was a suitable scaffold material for tissue engineering purposes and co-cultivation procedures.
authors: Aachen University of Applied Sciences, FB 9 Department of Biomedical Engineering, Laboratory of Medical and Molecular Biology, Jülich, Germany.
authors: Volker
authors: V
authors: Seifarth
authors: Matthias
authors: M
authors: Gossmann
authors: Heinz Peter
authors: HP
authors: Janke
authors: Joachim O
authors: JO
authors: Grosse
authors: Christoph
authors: C
authors: Becker
authors: Ingo
authors: I
authors: Heschel
authors: Gerhard M
authors: GM
authors: Artmann
authors: Aysegül
authors: A
authors: Temiz Artmann
mesh_terms: D000818:Animals; D019149:Bioreactors; D002245:Carbon Dioxide / Q000737:chemistry; D018920:Coculture Techniques; D004581:Electronics; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D044968:Regenerative Medicine / Q000379:methods; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014513:Ureter / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery
chemical_list: D002245:Carbon Dioxide