title: Association between genetic variants on chromosome 15q25 locus and several nicotine dependence traits in Polish population: a case-control study.
abstract: Tobacco smoking continues to be a leading cause of disease and mortality. Recent research has confirmed the important role of nicotinic acetylcholine receptor (nAChR) gene cluster on chromosome 15q 24-25 in nicotine dependence and smoking. In this study we tested the association of smoking initiation, age at onset of daily smoking, and heaviness of smoking with five single nucleotide polymorphisms (SNPs) within the CHRNA5-CHRNA3-CHRNB4 cluster. The group of 389 adult subjects of European ancestry from the north of Poland, including 212 ever (140 current and 72 former) and 177 never smokers with mean age 49.26, was genotyped for rs16969868, rs1051730, rs588765, rs6495308, and rs578776 polymorphisms. Distributions of genotypes for rs16969868 and rs1051730 were identical so they were analyzed together. Further analysis revealed the association between rs16969868-1051730 (OR = 2.66; 95% CI: 1.30-5.42) and number of cigarettes smoked per day (CPD) with heaviness of nicotine addiction measured by the Fagerström Test for Nicotine Dependence (FTND) (OR = 2.60; 95% CI: 1.24-5.43). No association between these polymorphisms and other phenotypes was found. Similarly, the association between rs588765, rs6495308, rs578776, and analyzed phenotypes was not confirmed. This study provides strong evidence for the role of the CHRNA5-CHRNA3-CHRNB4 cluster in heaviness of nicotine addiction.
authors: Department of Family Medicine, Nicolaus Copernicus University in Torun, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
authors: Krzysztof
authors: 0000-0002-7700-0952
authors: K
authors: Buczkowski
authors: Department of Allergology, Chair of Lung Disease, Medical University of Gdansk, Dębinki 7, 80-211 Gdańsk, Poland.
authors: Alicja
authors: A
authors: Sieminska
authors: Department of Molecular and Forensic Genetics, Institute of Forensic Medicine, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
authors: Katarzyna
authors: K
authors: Linkowska
authors: Department of Family Medicine, Nicolaus Copernicus University in Torun, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
authors: Slawomir
authors: 0000-0002-8055-4833
authors: S
authors: Czachowski
authors: Department of Pulmonary Diseases and Tuberculosis, Nicolaus Copernicus University in Torun, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Seminaryjna 1, 85-326 Bydgoszcz, Poland.
authors: Grzegorz
authors: G
authors: Przybylski
authors: Department of Allergology, Chair of Lung Disease, Medical University of Gdansk, Dębinki 7, 80-211 Gdańsk, Poland.
authors: Ewa
authors: E
authors: Jassem
authors: Department of Molecular and Forensic Genetics, Institute of Forensic Medicine, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
authors: Tomasz
authors: T
authors: Grzybowski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011044:Poland; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics*; D012907:Smoking / Q000235:genetics; D014029:Tobacco Use Disorder / Q000235:genetics*; D055815:Young Adult
references: Nat Genet. 2010 May;42(5):441-7
references: 20418890
references: Am J Med Genet B Neuropsychiatr Genet. 2012 Mar;159B(2):227-35
references: 22223462
references: Behav Genet. 1995 Mar;25(2):103-17
references: 7733853
references: Addiction. 2008 Sep;103(9):1544-52
references: 18783506
references: Neuropharmacology. 2012 Nov;63(6):1002-11
references: 22820273
references: Addict Biol. 2010 Jul;15(3):346-57
references: 20624154
references: Am J Med Genet B Neuropsychiatr Genet. 2009 Oct 5;150B(7):926-33
references: 19132693
references: Addiction. 2010 Nov;105(11):2014-22
references: 20840187
references: Addiction. 2002 Aug;97(8):1063-70
references: 12144609
references: Mol Pharmacol. 2011 Jan;79(1):119-25
references: 20881005
references: Int J Mol Epidemiol Genet. 2013 Jun 25;4(2):109-19
references: 23875064
references: Hum Mol Genet. 2009 Oct 15;18(20):4007-12
references: 19628476
references: PLoS Genet. 2010 Aug;6(8). pii: e1001053. doi: 10.1371/journal.pgen.1001053
references: 20700436
references: Psychopharmacology (Berl). 1995 Jan;117(1):2-10; discussion 14-20
references: 7724697
references: Am J Med Genet B Neuropsychiatr Genet. 2010 Apr 5;153B(3):745-56
references: 19859904
references: Health Psychol. 1996 Nov;15(6):478-84
references: 8973929
references: N Engl J Med. 1992 Sep 17;327(12):829-33
references: 1508241
references: Nicotine Tob Res. 2012 Jun;14(6):720-33
references: 22241830
references: Trends Pharmacol Sci. 2006 Sep;27(9):482-91
references: 16876883
references: Arch Gen Psychiatry. 2001 Sep;58(9):810-6
references: 11545662
references: Lancet. 2005 Nov 19;366(9499):1784-93
references: 16298215
references: PLoS One. 2010;5(8):e12183
references: 20808433
references: J Natl Cancer Inst. 2012 May 16;104(10):740-8
references: 22534784
references: PLoS Genet. 2008 Jul;4(7):e1000125
references: 18618000
references: Cancer Epidemiol Biomarkers Prev. 2008 Dec;17(12):3517-25
references: 19029397
references: Nat Genet. 2010 May;42(5):448-53
references: 20418888
references: Trends Pharmacol Sci. 2010 Jan;31(1):46-51
references: 19896728
references: J Natl Cancer Inst. 2008 Nov 5;100(21):1552-6
references: 18957677
references: Cancer Res. 2009 Sep 1;69(17):6848-56
references: 19706762
references: N Engl J Med. 2010 Jun 17;362(24):2295-303
references: 20554984
references: Curr Biol. 2008 Aug 26;18(16):1241-8
references: 18691889
references: Tob Control. 1997;6 Suppl 2:S57-62
references: 9583654
references: Br J Addict. 1991 Sep;86(9):1119-27
references: 1932883
references: Adv Genet. 2008;62:1-32
references: 19010252
references: Int J Epidemiol. 2010 Apr;39(2):563-77
references: 19776245
references: Nicotine Tob Res. 2011 Dec;13(12):1167-75
references: 22071378
references: Ann N Y Acad Sci. 2003 Sep;998:41-52
references: 14592862
references: J Addict Dis. 2005;24(1):85-100
references: 15774413
references: Hum Mol Genet. 2009 Aug 1;18(15):2922-7
references: 19429911
references: Eur J Cancer Prev. 2012 Mar;21(2):111-7
references: 21955800
references: PLoS Med. 2006 Nov;3(11):e442
references: 17132052
references: Addiction. 2009 Oct;104(10):1731-1740
references: 20871796
references: PLoS Genet. 2008 Jul;4(7):e1000114
references: 18797516
references: Am J Psychiatry. 2005 May;162(5):924-30
references: 15863794
references: Nature. 2008 Apr 3;452(7187):638-42
references: 18385739
references: Science. 2004 Nov 5;306(5698):1029-32
references: 15528443
references: Am J Med Genet B Neuropsychiatr Genet. 2009 Jun 5;150B(4):453-66
references: 19259974
references: Annu Rev Med. 1996;47:493-507
references: 8712799
references: Hum Mol Genet. 2007 Jan 1;16(1):36-49
references: 17135278
references: Am J Psychiatry. 2003 Jun;160(6):1041-52
references: 12777258
references: Nat Genet. 2010 May;42(5):436-40
references: 20418889
references: Biol Psychiatry. 2008 Jun 1;63(11):1039-46
references: 18163978
references: Mol Psychiatry. 2008 Apr;13(4):368-73
references: 18227835
references: Hum Genet. 2010 Nov;128(5):491-9
references: 20725741
references: Am J Psychiatry. 2008 Sep;165(9):1163-71
references: 18519524
references: Am J Hum Genet. 2012 Jan 13;90(1):7-24
references: 22243964
references: PLoS One. 2011;6(10):e26668
references: 22046326
references: PLoS One. 2009;4(2):e4653
references: 19247474
references: Cancer Epidemiol Biomarkers Prev. 2006 Oct;15(10):1825-8
references: 17035388
references: Neuropsychopharmacology. 2010 Nov;35(12):2392-402
references: 20736995
references: Br J Addict. 1989 Jul;84(7):791-9
references: 2758152
references: Respir Res. 2011;12:9
references: 21232152
references: Hum Mol Genet. 2007 Jan 1;16(1):24-35
references: 17158188