title: Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte-CB processing system.
abstract: BACKGROUND
  Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow (BM)-derived mesenchymal stem cells (MSC), and for MSC expansion in culture.
  
  
  OBJECTIVE
  The purpose of the study was to evaluate the ability of the PrepaCyte-CB processing system to reduce volume, deplete RBC, and recover mononuclear cells (MNC) from equine BM specimens.
  
  
  METHODS
  One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre- and post-PrepaCyte-CB processing. Volume and RBC reduction, and total nucleated cell (TNC) and MNC recoveries were determined.
  
  
  RESULTS
  Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1% (range, 62.0-96.7%). The median preprocessing total TNC count was 2.2 × 10(9) (range, 0.46-7.9 × 10(9)) and the median postprocessing TNC count was 1.7 × 10(9) (range, 0.3-4.4 × 10(9); P < .0001), with a median recovery of 73.5% (range, 22.4-216.7%). The median preprocessing total MNC count was 0.9 × 10(9) (range, 0.1-4.7 × 10(9)) and median postprocessing total MNC count was 0.8 × 10(9) (range, 0.1-2.7 × 10(9); P = .06), with a median recovery of 83.7% (range, 15.4-413.9%).
  
  
  CONCLUSIONS
  The PrepaCyte-CB processing system can be used to deplete both volume and RBC, and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte-CB processing system are warranted.
authors: Veterinary Medical Teaching Hospital, University of California-Davis, Davis, CA, USA.
authors: Andrew G
authors: AG
authors: Burton
authors: Regenerative Medicine Laboratory, University of California-Davis, Davis, CA, USA.
authors: Kaitlin C
authors: KC
authors: Clark
authors: Department of Pathology, Microbiology and Immunology, University of California-Davis, Davis, CA, USA.
authors: Dori L
authors: DL
authors: Borjesson
authors: Department of Pathology, Microbiology and Immunology, University of California-Davis, Davis, CA, USA.
authors: Danielle D
authors: DD
authors: Carrade
authors: Regenerative Medicine Laboratory, University of California-Davis, Davis, CA, USA.
authors: Julie
authors: J
authors: Burges
authors: Department of Pathology, Microbiology and Immunology, University of California-Davis, Davis, CA, USA.
authors: Sean D
authors: SD
authors: Owens
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000737:chemistry*; D016465:Bone Marrow Purging / Q000295:instrumentation / Q000662:veterinary*; D002469:Cell Separation / Q000295:instrumentation / Q000662:veterinary*; D004906:Erythrocyte Count / Q000662:veterinary; D004911:Erythrocyte Volume / Q000662:veterinary; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology; D006736:Horses / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000502:physiology; D013048:Specimen Handling
keywords: Cell separation; horses; mesenchymal stem cell; regenerative