title: Near-infrared spectroscopy as a diagnostic tool for distinguishing between normal and malignant colorectal tissues.
abstract: Cancer diagnosis is one of the most important tasks of biomedical research and has become the main objective of medical investigations. The present paper proposed an analytical strategy for distinguishing between normal and malignant colorectal tissues by combining the use of near-infrared (NIR) spectroscopy with chemometrics. The successive projection algorithm-linear discriminant analysis (SPA-LDA) was used to seek a reduced subset of variables/wavenumbers and build a diagnostic model of LDA. For comparison, the partial least squares-discriminant analysis (PLS-DA) based on full-spectrum classification was also used as the reference. Principal component analysis (PCA) was used for a preliminary analysis. A total of 186 spectra from 20 patients with partial colorectal resection were collected and divided into three subsets for training, optimizing, and testing the model. The results showed that, compared to PLS-DA, SPA-LDA provided more parsimonious model using only three wavenumbers/variables (4065, 4173, and 5758 cm(-1)) to achieve the sensitivity of 84.6%, 92.3%, and 92.3% for the training, validation, and test sets, respectively, and the specificity of 100% for each subset. It indicated that the combination of NIR spectroscopy and SPA-LDA algorithm can serve as a potential tool for distinguishing between normal and malignant colorectal tissues.
authors: Yibin University Hospital, Yibin, Sichuan 644000, China ; Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China.
authors: Hui
authors: H
authors: Chen
authors: Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China ; The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Zan
authors: Z
authors: Lin
authors: The Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan 637000, China.
authors: Lin
authors: L
authors: Mo
authors: Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China.
authors: Tong
authors: T
authors: Wu
authors: Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China.
authors: Chao
authors: C
authors: Tan
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D016002:Discriminant Analysis; D006801:Humans; D016018:Least-Squares Analysis; D008875:Middle Aged; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
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