title: Quantification of polygalasaponin F in rat plasma using liquid chromatography-tandem mass spectrometry and its pharmacokinetics application.
abstract: A rapid and highly selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for determination of polygalasaponin F (PF) in rat plasma was developed and validated. The chromatographic separation was achieved on a reverse-phase Zorbax SB-C18 column (150 × 4.6 mm, 5 µm), using 2 mm ammonium acetate (pH adjusted to 6.0 with acetic acid) and acetonitrile (25:75, v/v) as a mobile phase at 30 °C. MS/MS detection was performed using an electrospray ionization operating in positive ion multiple reaction monitoring mode by monitoring the ion transitions from m/z 1091.5 → 471.2 (PF) and m/z 700.4 → 235.4 (internal standard), respectively. The calibration curve showed a good linearity in the concentration range 0.0544-13.6 µg/mL, with a limit of quantification of 0.0544 µg/mL. The intra- and inter-day precisions were <9.7% in rat plasma. The method was validated as per US Food and Drug Administration guidelines and successfully applied to pharmacokinetic study of PF in rats.
authors: Department of Pediatrics, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Wenli
authors: W
authors: Zhou
authors: Department of Anus Colorectal Surgery, the First Hospital of Jilin University Branch, Changchun, 130021, China.
authors: Jiantao
authors: J
authors: Zhang
authors: Department of Pediatrics, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Wei
authors: W
authors: Xu
authors: Department of Pediatrics, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Jinghui
authors: J
authors: Sun
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D031801:Polygala / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C573752:polygalasaponin F
keywords: LC-MS/MS; Polygala japonica; pharmacokinetics; polygalasaponin F; quantitative analysis