title: An RPTPα/Src family kinase/Rap1 signaling module recruits myosin IIB to support contractile tension at apical E-cadherin junctions.
abstract: Cell-cell adhesion couples the contractile cortices of epithelial cells together, generating tension to support a range of morphogenetic processes. E-cadherin adhesion plays an active role in generating junctional tension by promoting actin assembly and cortical signaling pathways that regulate myosin II. Multiple myosin II paralogues accumulate at mammalian epithelial cell-cell junctions. Earlier, we found that myosin IIA responds to Rho-ROCK signaling to support junctional tension in MCF-7 cells. Although myosin IIB is also found at the zonula adherens (ZA) in these cells, its role in junctional contractility and its mode of regulation are less well understood. We now demonstrate that myosin IIB contributes to tension at the epithelial ZA. Further, we identify a receptor type-protein tyrosine phosphatase alpha-Src family kinase-Rap1 pathway as responsible for recruiting myosin IIB to the ZA and supporting contractile tension. Overall these findings reinforce the concept that orthogonal E-cadherin-based signaling pathways recruit distinct myosin II paralogues to generate the contractile apparatus at apical epithelial junctions.
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia g.gomez@uq.edu.au a.yap@uq.edu.au.
authors: Guillermo A
authors: GA
authors: Gomez
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Robert W
authors: RW
authors: McLachlan
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Selwin K
authors: SK
authors: Wu
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Benjamin J
authors: BJ
authors: Caldwell
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Elliott
authors: E
authors: Moussa
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Suzie
authors: S
authors: Verma
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Michele
authors: M
authors: Bastiani
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Rashmi
authors: R
authors: Priya
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Robert G
authors: RG
authors: Parton
authors: UNSW Australia, ARC Centre of Excellence in Advanced Molecular Imaging and Australian Centre for Nanomedicine, Sydney 2052, Australia.
authors: Katharina
authors: K
authors: Gaus
authors: Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate, UMR 7216 CNRS Bâtiment Lamarck, F-75205 Paris Cedex 13, France.
authors: Jan
authors: J
authors: Sap
authors: Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia g.gomez@uq.edu.au a.yap@uq.edu.au.
authors: Alpha S
authors: AS
authors: Yap
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4 / Q000378:metabolism*; D015398:Signal Transduction; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D015820:Cadherins; D019061:src-Family Kinases; C516841:PTPRA protein, human; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D024501:Nonmuscle Myosin Type IIB; D020827:rap1 GTP-Binding Proteins
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