title: Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.
abstract: The ubiX gene of Colwellia psychrerythraea strain 34H encodes a 3-octaprenyl-4-hydroxybenzoate carboxylase (CpsUbiX, UniProtKB code: Q489U8) that is involved in the third step of the ubiquinone biosynthesis pathway and harbors a flavin mononucleotide (FMN) as a potential cofactor. Here, we report the crystal structures of two forms of CpsUbiX: an FMN-bound wild type form and an FMN-unbound V47S mutant form. CpsUbiX is a dodecameric enzyme, and each monomer possesses a typical Rossmann-fold structure. The FMN-binding domain of UbiX is composed of three neighboring subunits. The highly conserved Gly15, Ser41, Val47, and Tyr171 residues play important roles in FMN binding. Structural comparison of the FMN-bound wild type form with the FMN-free form reveals a significant conformational difference in the C-terminal loop region (comprising residues 170-176 and 195-206). Subsequent computational modeling and liposome binding assay both suggest that the conformational flexibility observed in the C-terminal loops plays an important role in substrate and lipid bindings. The crystal structures presented in this work provide structural framework and insights into the catalytic mechanism of CpsUbiX.
authors: Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea.
authors: Hackwon
authors: H
authors: Do
authors: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors: Soo Jin
authors: SJ
authors: Kim
authors: 1] Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea [2] Department of Polar Sciences, Korea University of Science and Technology, Incheon 406-840, Republic of Korea.
authors: Chang Woo
authors: CW
authors: Lee
authors: 1] Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea [2] Department of Polar Sciences, Korea University of Science and Technology, Incheon 406-840, Republic of Korea.
authors: Han-Woo
authors: HW
authors: Kim
authors: Department of Biochemistry, School of Biotechnology and Graduate School of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
authors: Hyun Ho
authors: HH
authors: Park
authors: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors: Ho Min
authors: HM
authors: Kim
authors: 1] Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea [2] Department of Polar Sciences, Korea University of Science and Technology, Incheon 406-840, Republic of Korea.
authors: Hyun
authors: H
authors: Park
authors: X-Ray Core, Scripps Florida, Jupiter, FL 33458, USA.
authors: HaJeung
authors: H
authors: Park
authors: 1] Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea [2] Department of Polar Sciences, Korea University of Science and Technology, Incheon 406-840, Republic of Korea.
authors: Jun Hyuck
authors: JH
authors: Lee
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D005486:Flavin Mononucleotide / Q000737:chemistry*; D020563:Gammaproteobacteria / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D055672:Static Electricity
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D005486:Flavin Mononucleotide; D002262:Carboxy-Lyases
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