title: BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea.
abstract: Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [B. cinerea MctA (BcMctA)] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China. zfcui@zjut.edu.cn.
authors: Zhifeng
authors: Z
authors: Cui
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors: Nana
authors: N
authors: Gao
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors: Qian
authors: Q
authors: Wang
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors: Yun
authors: Y
authors: Ren
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors: Kun
authors: K
authors: Wang
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China. thzhu@zjut.edu.cn.
authors: Tingheng
authors: T
authors: Zhu
mesh_terms: D019342:Acetic Acid / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D020171:Botrytis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D018553:Cucumis sativus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D019344:Lactic Acid / Q000378:metabolism / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D019289:Pyruvic Acid / Q000378:metabolism / Q000494:pharmacology; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D027501:Monocarboxylic Acid Transporters; D019344:Lactic Acid; D019289:Pyruvic Acid; D019342:Acetic Acid
keywords: Botrytis cinerea; Functional analysis; Gene disruption; Monocarboxylate transporter
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