title: Identification and functional characterization of voltage-gated sodium channels in lymphocytes.
abstract: A variety of ion channels has been discovered in lymphocytes. RT-PCR and real-time PCR analysis revealed that ALL (acute lymphocytic leukemia) cell lines and human peripheral blood mononuclear cells mainly expressed TTX (tetrodotoxin)-sensitive voltage-gated sodium channels (VGSCs). Expression of VGSC protein was confirmed by western blots and Immunofluorescence. Whole-cell patch-clamp recordings showed that a sub-population (20%) of MOLT-4 cells expressed functional VGSCs, which were TTX-sensitive. Importantly, 2 μM TTX decreased the invasion of Jurkat and MOLT-4 cells ∼90%. These results indicate that the activity of VGSCs could represent a novel mechanism potentiating the invasive capacity of these cells.
authors: Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou 350004, PR China.
authors: Weifeng
authors: W
authors: Huang
authors: Department of Blood Transfusion, Maternal and Child Health Hospital of Xiamen, Xiamen 361003, PR China.
authors: Chunjing
authors: C
authors: Lu
authors: Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou 350004, PR China.
authors: Yong
authors: Y
authors: Wu
authors: Xiamen Medical Research Institute, Xiamen 361008, PR China.
authors: Shou
authors: S
authors: Ouyang
authors: Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou 350004, PR China. Electronic address: 58301694@qq.com.
authors: Yuanzhong
authors: Y
authors: Chen
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D008213:Lymphocyte Activation / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*; D061566:Voltage-Gated Sodium Channels / Q000737:chemistry* / Q000145:classification / Q000502:physiology*
chemical_list: D061566:Voltage-Gated Sodium Channels; D012964:Sodium
keywords: Invasion; Lymphocytes; Voltage-gated sodium channel