title: Resin penetration in artificial enamel carious lesions after using sodium hypochlorite as a deproteinization agent.
abstract: OBJECTIVE
  To study the degree of penetration of an adhesive resin in artificial enamel carious lesions after using sodium hypochlorite as deproteinization agent.
  
  
  METHODS
  Twenty included human third-molars, extracted for surgical indication, were used. Artificial lesions were created in the buccal and lingual sides of each specimen through a cycle of demineralization-remineralization. Samples were then incubated in human saliva for 7 days at 37 ° C. After surface cleaning, lesions and the peripheral sound enamel were etched with 37% orthophosphoric acid for 20 seconds. One lesion of each specimen was treated with 5.25% sodium hypochlorite (NaOCl) for one minute. The other lesion of each specimen was used as a control. Experimental and control lesions were sealed with a fluid resin marked with Rhodamine B. Lesions were sectioned for microscopic observation by epifluorescence and polarized light. The images obtained were analyzed morphometrically. The micrometer measurements were made with ImageJ ® software. The level of significance was assessed at p<0.05.
  
  
  RESULTS
  The average sealant depth penetration in the control group was 94.9 ± 28.6 μm versus 122.8 ± 25.3 μm in the experimental group. This represents Δ 20.1% significantly greater penetration when using sodium hypochlorite (p<0.001).
  
  
  CONCLUSIONS
  The results demonstrated a significant penetration of the sealing resin when the conventional technique is complemented with the application of 5.25% sodium hypochlorite for one minute in artificial enamel carious lesions.
authors: Department of Preventive Dentistry, Facultad de Odontología Universidad de Valparaíso, Valparaíso, Chile.
authors: Ss
authors: S
authors: Gómez
authors: Department of Preventive Dentistry, Facultad de Odontología Universidad de Valparaíso, Valparaíso, Chile.
authors: P
authors: P
authors: Bravo
authors: Department of Preventive Dentistry, Facultad de Odontología Universidad de Valparaíso, Valparaíso, Chile.
authors: R
authors: R
authors: Morales
authors: Department of Preventive Dentistry, Facultad de Odontología Universidad de Valparaíso, Valparaíso, Chile.
authors: A
authors: A
authors: Romero
authors: Departament of Basic Science, Facultad de Odontología Universidad Finis Terrae, Santiago, Chile.
authors: A
authors: A
authors: Oyarzún
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D000293:Adolescent; D003731:Dental Caries / Q000473:pathology*; D003743:Dental Enamel / Q000187:drug effects* / Q000648:ultrastructure; D003764:Dental Materials / Q000737:chemistry*; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D010756:Phosphoric Acids / Q000737:chemistry; D011489:Protein Denaturation; D012235:Rhodamines; D012463:Saliva / Q000502:physiology; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D055815:Young Adult
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; D003764:Dental Materials; D005456:Fluorescent Dyes; D010756:Phosphoric Acids; D012235:Rhodamines; C040415:heliobond; D012973:Sodium Hypochlorite; C030242:phosphoric acid; C029773:rhodamine B
keywords: artificial carious lesions; epifluorescence microscopy; resin penetration; sodium hypochlorite