title: Correlation of NADH fluorescence lifetime and oxidative phosphorylation metabolism in the osteogenic differentiation of human mesenchymal stem cell.
abstract: Reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime has been broadly used as a metabolic indicator for stem cell imaging. However, the direct relationship between NADH fluorescence lifetime and metabolic pathway and activity remains to be clarified. In this study, we measured the NADH fluorescence lifetime of human mesenchymal stem cells (hMSCs) as well as the metabolic indictors, such as adenosine triphosphate (ATP) level, oxygen consumption, and lactate release, up to 4 weeks under normal osteogenic differentiation and oxidative phosphorylation-attenuated/inhibited differentiation by oligomycin A (OA) treatment. NADH fluorescence lifetime was positively correlated with oxygen consumption and ATP level during energy transformation from glycolysis to oxidative phosphorylation. Under OA treatment, oxidative phosphorylation was attenuated/inhibited (i.e., oxygen consumption remained the same as controls or lower), cells showed attenuated differentiation under glycolysis, and NADH fluorescence lifetime change was not detected. Increased expression of the overall complex proteins was observed in addition to Complex I. We suggested special caution needs to be exercised while interpreting NADH fluorescence lifetime signal in terms of stemcell differentiation.
authors: National Yang-Ming University, Institute of Biophotonics, 155 Li-Nong Street, Section 2, Taipei 112, TaiwanbNational Taiwan University, Department of Physics, 1 Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors: Han-Wen
authors: HW
authors: Guo
authors: National Yang-Ming University, Institute of Biophotonics, 155 Li-Nong Street, Section 2, Taipei 112, Taiwan.
authors: Jia-Sin
authors: JS
authors: Yu
authors: National Yang-Ming University, Department of Biochemistry and Molecular Biology, 155 Li-Nong Street, Section 2, Taipei 112, Taiwan.
authors: Shu-Han
authors: SH
authors: Hsu
authors: National Yang-Ming University, Department of Biochemistry and Molecular Biology, 155 Li-Nong Street, Section 2, Taipei 112, TaiwandMackay Medical College, Department of Medicine, 46 Zhong-Zheng Road, Section 3, San-Jhih, New Taipei City 252, Taiwan.
authors: Yau-Huei
authors: YH
authors: Wei
authors: National Yang-Ming University, Institute of Biophotonics, 155 Li-Nong Street, Section 2, Taipei 112, TaiwaneNational Yang-Ming University, Institute of Clinical Medicine, 155 Li-Nong Street, Section 2, Taipei 112, Taiwan.
authors: Oscar K
authors: OK
authors: Lee
authors: National Taiwan University, Department of Physics, 1 Roosevelt Road, Section 4, Taipei 106, TaiwanfNational Taiwan University, Center for Quantum Science and Engineering, 1 Roosevelt Road, Section 4, Taipei 106, TaiwangNational Taiwan University, Center f.
authors: Chen-Yuan
authors: CY
authors: Dong
authors: National Yang-Ming University, Institute of Biophotonics, 155 Li-Nong Street, Section 2, Taipei 112, TaiwanhUniversity of Maryland, Fischell Department of Bioengineering, College Park, Maryland 20742, United States.
authors: Hsing-Wen
authors: HW
authors: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009243:NAD / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption
chemical_list: D009840:Oligomycins; C031004:oligomycin A; D009243:NAD; D019344:Lactic Acid; D000255:Adenosine Triphosphate