title: [Acute promyelocytic leukaemia with translocations of t(15;17)(q22;q21) and rob(13;21): a case report and literatures review].
abstract: OBJECTIVE
  To report an acute promyelocytic leukaemia (APL) case with translocation of rob (13;21) t(15;17) (q22;q21) and review its clinical and laboratory characteristics.
  
  
  METHODS
  Based on routine karyotype analysis and bone marrow morphology, we further used double color double fluorescent in situ hybridization (DCDF-FISH) and reverse transcriptase PCR (RT-PCR) to examine the patient's abnormities on cytogenetic and molecular biology, and reveal the clinical characteristics of this rare translocation also from the related literatures.
  
  
  RESULTS
  The clinical manifestation and bone marrow morphology examination of this patient were in accordance with pathologic feature of APL. On first visit, immunophenotyping analysis showed positive myeloid markers. Through R-banding, the patient's karyotype was confirmed as 45, XX, rob(13;21) t(15;17) (q22;q21) [6]/45, XX, rob(13;21) [14]. FISH results showed that 68.9% cells were typical t(15;17) pattern. The positive rates of fusion gene of PML-RARα detected by RT-PCR was 25.8%. Patient was treated by induction and consolidation therapy, the karyotype was 45, XX, rob(13;21 )[20] after complete remission. The positive rate of fusion gene of PML-RARα by FISH and its level were 2.5% and 0.003% respectively.
  
  
  CONCLUSIONS
  APL with rob (13;21) t(15;17) (q22;q21) was very rare, which was accorded with clinical and laboratory characteristics of APL. The value of chromosome abnormality as a prognostic marker in APL needs to be further observed..
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Haiya
authors: H
authors: Zheng
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Shenglan
authors: S
authors: Gong
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Mengqiao
authors: M
authors: Guo
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Xianmin
authors: X
authors: Song
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Min
authors: M
authors: Liu
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Hui
authors: H
authors: Cheng
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Xiaoxia
authors: X
authors: Hu
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors: Jianmin
authors: J
authors: Wang
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D041321:Chromosomes, Human, X; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D015473:Leukemia, Promyelocytic, Acute; D015514:Oncogene Proteins, Fusion; D012074:Remission Induction; D014178:Translocation, Genetic
chemical_list: D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
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