title: Identification of Coq11, a new coenzyme Q biosynthetic protein in the CoQ-synthome in Saccharomyces cerevisiae.
abstract: Coenzyme Q (Q or ubiquinone) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail and is required for mitochondrial electron transport. In the yeast Saccharomyces cerevisiae, Q is synthesized by the products of 11 known genes, COQ1-COQ9, YAH1, and ARH1. The function of some of the Coq proteins remains unknown, and several steps in the Q biosynthetic pathway are not fully characterized. Several of the Coq proteins are associated in a macromolecular complex on the matrix face of the inner mitochondrial membrane, and this complex is required for efficient Q synthesis. Here, we further characterize this complex via immunoblotting and proteomic analysis of tandem affinity-purified tagged Coq proteins. We show that Coq8, a putative kinase required for the stability of the Q biosynthetic complex, is associated with a Coq6-containing complex. Additionally Q6 and late stage Q biosynthetic intermediates were also found to co-purify with the complex. A mitochondrial protein of unknown function, encoded by the YLR290C open reading frame, is also identified as a constituent of the complex and is shown to be required for efficient de novo Q biosynthesis. Given its effect on Q synthesis and its association with the biosynthetic complex, we propose that the open reading frame YLR290C be designated COQ11.
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Christopher M
authors: CM
authors: Allan
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Agape M
authors: AM
authors: Awad
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Jarrett S
authors: JS
authors: Johnson
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Dyna I
authors: DI
authors: Shirasaki
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Charles
authors: C
authors: Wang
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute.
authors: Crysten E
authors: CE
authors: Blaby-Haas
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute, the UCLA/DOE Institute for Genomics and Proteomics, University of California, Los Angeles, California 90095.
authors: Sabeeha S
authors: SS
authors: Merchant
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute, the Department of Biological Chemistry, and the UCLA/DOE Institute for Genomics and Proteomics, University of California, Los Angeles, California 90095.
authors: Joseph A
authors: JA
authors: Loo
authors: From the Department of Chemistry and Biochemistry and the Molecular Biology Institute, cathy@chem.ucla.edu.
authors: Catherine F
authors: CF
authors: Clarke
mesh_terms: D002853:Chromatography, Liquid; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014451:Ubiquinone / Q000096:biosynthesis*
chemical_list: C000598214:Coq11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014451:Ubiquinone
keywords: Coenzyme Q; Immunoprecipitation; Mass Spectrometry (MS); Mitochondrial Metabolism; Protein Complex; Proteomics; Q Biosynthetic Intermediates; Saccharomyces cerevisiae; Ubiquinone; Yeast
references: J Biol Chem. 2005 Jan 28;280(4):2676-81
references: 15548532
references: J Biol Chem. 2005 May 27;280(21):20231-8
references: 15792955
references: Methods Mol Biol. 2006;313:107-20
references: 16118429
references: J Biol Chem. 2005 Sep 9;280(36):31397-404
references: 16027161
references: J Biol Chem. 2005 Dec 30;280(52):42627-35
references: 16230336
references: Mol Cell Proteomics. 2006 Jan;5(1):144-56
references: 16219938
references: Plant Physiol. 2006 Mar;140(3):984-97
references: 16461379
references: Mol Cell Proteomics. 2006 Apr;5(4):589-607
references: 16399765
references: J Biol Chem. 2006 Apr 21;281(16):11225-34
references: 16414959
references: J Biol Chem. 2006 Jun 16;281(24):16401-9
references: 16624818
references: PLoS Genet. 2006 Oct 20;2(10):e170
references: 17054397
references: Mitochondrion. 2007 Jun;7 Suppl:S62-71
references: 17482885
references: Arch Biochem Biophys. 2007 Jul 1;463(1):19-26
references: 17391640
references: Arch Biochem Biophys. 2007 Nov 15;467(2):144-53
references: 17889824
references: PLoS One. 2008;3(4):e1994
references: 18431481
references: Yeast. 1998 Jun 30;14(9):839-46
references: 9818721
references: Gene. 1999 Jun 11;233(1-2):197-203
references: 10375636
references: J Biol Chem. 1999 Jul 30;274(31):21665-72
references: 10419476
references: Science. 1999 Jul 30;285(5428):751-3
references: 10427000
references: Science. 1999 Aug 6;285(5429):901-6
references: 10436161
references: Plant Mol Biol. 1999 Jul;40(5):795-806
references: 10487214
references: FEBS J. 2008 Jul;275(13):3352-61
references: 18494801
references: J Cell Biol. 2008 Sep 8;182(5):937-50
references: 18779372
references: FEMS Yeast Res. 2008 Dec;8(8):1263-75
references: 18801050
references: Nucleic Acids Res. 2009 Jan;37(Database issue):D969-74
references: 18832363
references: Biochim Biophys Acta. 2009 Jan;1791(1):69-75
references: 19022396
references: Cell Mol Life Sci. 2009 Jan;66(1):173-86
references: 19002377
references: FEBS J. 2009 Feb;276(3):748-59
references: 19120452
references: PLoS One. 2009;4(2):e4345
references: 19190775
references: Proteomics. 2009 Mar;9(6):1683-95
references: 19294628
references: Science. 2010 Jan 22;327(5964):425-31
references: 20093466
references: J Biosci Bioeng. 2010 Jun;109(6):564-9
references: 20471595
references: Biochem Biophys Res Commun. 2010 May 21;396(1):74-9
references: 20494114
references: Chem Biol. 2010 May 28;17(5):449-59
references: 20534343
references: J Biol Chem. 2010 Sep 3;285(36):27827-38
references: 20592037
references: Dev Disabil Res Rev. 2010;16(2):200-18
references: 20818735
references: Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9
references: 21109532
references: Biochim Biophys Acta. 2011 May;1811(5):348-60
references: 21296186
references: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1184-8
references: 22102023
references: J Exp Bot. 2012 Feb;63(4):1609-18
references: 22371323
references: Plant Physiol. 2012 Mar;158(3):1172-92
references: 22274653
references: J Biol Chem. 2012 Jul 6;287(28):23571-81
references: 22593570
references: Neuron. 2012 Jul 12;75(1):41-57
references: 22794259
references: Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1050-5
references: 10655482
references: J Biol Chem. 2001 Jan 12;276(2):1503-9
references: 11035018
references: J Biol Chem. 2001 May 25;276(21):18161-8
references: 11279158
references: Biochemistry. 2001 Jun 12;40(23):6836-44
references: 11389597
references: Biochem J. 2001 Aug 1;357(Pt 3):749-57
references: 11463345
references: Arch Biochem Biophys. 2001 Aug 1;392(1):48-58
references: 11469793
references: J Biol Chem. 1997 Apr 4;272(14):9182-8
references: 9083049
references: J Biol Chem. 1997 Jul 25;272(30):19088-94
references: 9228094
references: Yeast. 1998 Jan 30;14(2):115-32
references: 9483801
references: Biochim Biophys Acta. 2012 Sep;1823(9):1531-52
references: 22569643
references: Trends Plant Sci. 2012 Sep;17(9):546-55
references: 22694836
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D348-52
references: 23197659
references: J Proteome Res. 2013 Jan 4;12(1):491-504
references: 23198870
references: PLoS One. 2013;8(1):e55041
references: 23365689
references: Biochim Biophys Acta. 2013 Apr;1831(4):776-91
references: 23270816
references: Mol Biol Evol. 2013 Dec;30(12):2725-9
references: 24132122
references: Nucleic Acids Res. 2014 Jan;42(Database issue):D191-8
references: 24253303
references: Biochim Biophys Acta. 2014 Apr 4;1841(4):630-44
references: 24406904
references: Biochimie. 2014 May;100:78-87
references: 24355204
references: Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4
references: 24753421
references: Biochemistry. 2014 Jun 24;53(24):3995-4003
references: 24870957
references: Biochim Biophys Acta. 2014 Nov;1841(11):1628-38
references: 25152161
references: Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):E4697-705
references: 25339443
references: Mol Cell. 2015 Jan 8;57(1):83-94
references: 25498144
references: Protein Expr Purif. 2015 Apr;108:13-7
references: 25540914
references: Biochemistry. 2001 Aug 21;40(33):9758-69
references: 11502169
references: J Biol Chem. 2002 Mar 22;277(12):9997-10002
references: 11788607
references: Nature. 2002 Jul 25;418(6896):387-91
references: 12140549
references: J Biol Chem. 2003 Jul 11;278(28):25308-16
references: 12721307
references: Biochim Biophys Acta. 2004 Jan 28;1660(1-2):171-99
references: 14757233
references: J Biol Chem. 2004 Mar 12;279(11):10052-9
references: 14701817
references: Nucleic Acids Res. 2004;32(5):1792-7
references: 15034147
references: Biochem Biophys Res Commun. 2004 Apr 30;317(2):648-53
references: 15063807
references: Protein Sci. 2004 Nov;13(11):3006-16
references: 15459342
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Nature. 1974 Jul 19;250(463):194-9
references: 4368490
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Proc Natl Acad Sci U S A. 1990 Jun;87(12):4879-83
references: 2352956
references: Microbiol Rev. 1990 Sep;54(3):211-25
references: 2215420
references: Crit Rev Biochem Mol Biol. 1994;29(3):165-97
references: 8070276
references: J Biol Chem. 1994 Oct 21;269(42):26144-51
references: 7929327
references: Methods Enzymol. 1995;260:213-23
references: 8592446
references: Yeast. 1996 Dec;12(15):1511-8
references: 8972574