title: Light-emitting diode irradiation promotes donor site wound healing of the free gingival graft.
abstract: BACKGROUND
  This study aims to evaluate the effect of light-emitting diode (LED) light irradiation on the donor wound site of the free gingival graft.
  
  
  METHODS
  Rat gingival fibroblasts were chosen to assess the cellular activities and in vitro wound healing with 0 to 20 J/cm(2) LED light irradiation. Seventy-two Sprague-Dawley rats received daily 0, 10 (low-dose [LD]), or 20 (high-dose [HD]) J/cm(2) LED light irradiation on the opened palatal wound and were euthanized after 4 to 28 days; the healing pattern was assessed by histology, histochemistry for collagen deposition, and immunohistochemistry for tumor necrosis factor (TNF)-α infiltration. The wound mRNA levels of heme oxygenase-1 (HO-1), TNF-α, the receptor for advanced glycation end products, vascular endothelial growth factor, periostin, Type I collagen, and fibronectin were also evaluated.
  
  
  RESULTS
  Cellular viability and wound closure were significantly promoted, and cytotoxicity was inhibited significantly using 5 J/cm(2) LED light irradiation in vitro. The wound closure, reepithelialization, and collagen deposition were accelerated, and sequestrum formation and inflammatory cell and TNF-α infiltration were significantly reduced in the LD group. HO-1 and TNF-α were significantly upregulated in the HD group, and most of the repair-associated genes were significantly upregulated in both the LD and HD groups at day 7. Persistent RAGE upregulation was noted in both the LD and HD groups until day 14.
  
  
  CONCLUSIONS
  LED light irradiation at 660 nm accelerated palatal wound healing, potentially via reducing reactive oxygen species production, facilitating angiogenesis, and promoting provisional matrix and wound reorganization.
authors: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
authors: Chen-Ying
authors: CY
authors: Wang
authors: Sheng-Chueh
authors: SC
authors: Tsai
authors: Min-Chen
authors: MC
authors: Yu
authors: Yu-Fang
authors: YF
authors: Lin
authors: Chih-Cheng
authors: CC
authors: Chen
authors: Po-Chun
authors: PC
authors: Chang
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005353:Fibronectins / Q000032:analysis; D005881:Gingiva / Q000502:physiology / Q000601:surgery*; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis; D008297:Male; D023421:Models, Animal; D010159:Palate / Q000502:physiology / Q000601:surgery*; D010789:Phototherapy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D062326:Re-Epithelialization / Q000502:physiology; D000067759:Receptor for Advanced Glycation End Products / Q000032:analysis; D060053:Transplant Donor Site / Q000502:physiology / Q000601:surgery*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
chemical_list: C000593874:Ager protein, rat; D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D005353:Fibronectins; C512836:Postn protein, rat; D000067759:Receptor for Advanced Glycation End Products; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat
keywords: Histology; laser therapy; low-level; periodontium; transplants; wound healing.