title: An in vivo comparison of antimicrobial efficacy of sodium hypochlorite and Biopure MTAD™ against enterococcus faecalis in primary teeth: A qPCR study.
abstract: OBJECTIVE
  Biopure MTAD™, a new root canal irrigant has shown promising results against the most common resistant microorganism, E. faecalis, in permanent teeth. However, there is lack of studies comparing its antimicrobial effectiveness with NaOCl in primary teeth. The purpose of this study was to compare the in vivo antimicrobial efficacy of NaOCl 2.5% and Biopure MTAD™ against E. faecalis in primary teeth.
  
  
  METHODS
  Forty non vital single rooted primary maxillary anterior teeth of children aged 4-8 years, were irrigated either with NaOCl 2.5% (n=15), Biopure MTAD™ (n=15) and 0.9% Saline (n=10, control group). Paper point samples were collected at baseline (S1) and after chemomechanical preparation (S2) during the pulpectomy procedure. The presence of E. faecalis in S1 & S2 was evaluated using Real time Polymerase Chain Reaction.
  
  
  RESULTS
  Statistical significant difference was found in the antimicrobial efficacy of NaOCl 2.5 % and BioPure MTAD™ when compared to saline (p>0.05). However, no statistical significant difference was found between the efficacies of both the irrigants.
  
  
  CONCLUSIONS
  NaOCl 2.5% and BioPure MTAD™, both irrigants are equally efficient against E. faecalis in necrotic primary anterior teeth. MTAD is a promising irrigant, however clinical studies are required to establish it as ideal root canal irrigant in clinical practice.
authors: Department of Pedodontics and Preventive Dentistry, Rajarajeswari Dental College and Hospital.
authors: S G
authors: SG
authors: Tulsani
authors: Department of Pedodontics and Preventive Dentistry, Rajarajeswari Dental College and Hospital.
authors: N
authors: N
authors: Chikkanarasaiah
authors: Department of Pedodontics and Preventive Dentistry, Rajarajeswari Dental College and Hospital.
authors: S
authors: S
authors: Bethur
mesh_terms: D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002648:Child; D002675:Child, Preschool; D019343:Citric Acid / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology / Q000628:therapy; D004318:Doxycycline / Q000627:therapeutic use*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D011136:Polysorbates / Q000627:therapeutic use*; D011670:Pulpectomy / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D014094:Tooth, Deciduous / Q000382:microbiology*
chemical_list: D000891:Anti-Infective Agents, Local; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D012973:Sodium Hypochlorite; D004318:Doxycycline
keywords: Biopure MTAD; Endodontic treatment; Irrigant; Primary teeth; Pulpectomy; Sodium hypochlorite