title: Deciphering allelic variations for seed glucosinolate traits in oilseed mustard (Brassica juncea) using two bi-parental mapping populations.
abstract: QTL mapping by two DH mapping populations deciphered allelic variations for five different seed glucosinolate traits in B. juncea. Allelic variations for five different seed glucosinolate (GS) traits, namely % propyl, % butyl, % pentyl, aliphatics and total GS content were studied through QTL analysis using two doubled haploid (DH) mapping populations. While the high GS parents in two populations differed in their profiles of seed aliphatic GS, the low GS parents were similar. Phenotypic data of seed GS traits from three environments of the two populations were subjected to QTL analysis. The first population (referred to as DE population) detected a total of 60 QTL from three environments which upon intra-population meta-QTL analysis were merged to 17 S-QTL (Stable QTL) and 15 E-QTL (Environment QTL). The second population (referred to as VH population) detected 58 QTL from the three environments that were merged to 15S-QTL and 16E-QTL. In both the populations, majority of S-QTL were detected as major QTL. Inter-population meta-analysis identified three C-QTL (consensus QTL) formed by merging major QTL from the two populations. Candidate genes of GS pathway were co-localized to the QTL regions either through genetic mapping or through in silico comparative analysis. Parental allelic variants of QTL or of the co-mapped candidate gene(s) were determined on the basis of the significantly different R (2) values of the component QTL from the two populations which were merged to form C-QTL. The results of the study are significant for marker-assisted transfer of the low GS trait and also for developing lines with lower GS than are present in Brassica juncea.
authors: Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors: Kadambini
authors: K
authors: Rout
authors: Manisha
authors: M
authors: Sharma
authors: Vibha
authors: V
authors: Gupta
authors: Arundhati
authors: A
authors: Mukhopadhyay
authors: Yaspal S
authors: YS
authors: Sodhi
authors: Deepak
authors: D
authors: Pental
authors: Akshay K
authors: AK
authors: Pradhan
mesh_terms: D000483:Alleles; D001947:Breeding; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005828:Genetics, Population; D005961:Glucosinolates / Q000737:chemistry*; D009149:Mustard Plant / Q000737:chemistry / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci; D012639:Seeds / Q000737:chemistry*
chemical_list: D005961:Glucosinolates
references: Genome. 2003 Oct;46(5):753-60
references: 14608392
references: Genetics. 2000 May;155(1):463-73
references: 10790417
references: Theor Appl Genet. 2003 Jun;107(1):168-80
references: 12835942
references: Plant Mol Biol. 2012 May;79(1-2):179-89
references: 22477389
references: Theor Appl Genet. 1995 Oct;91(5):802-8
references: 24169920
references: Plant Biotechnol J. 2013 Sep;11(7):855-66
references: 23721233
references: Theor Appl Genet. 2003 Apr;106(6):1116-21
references: 12671761
references: Plant Physiol. 2007 Feb;143(2):970-86
references: 17189332
references: Gene. 2011 Nov 10;487(2):135-42
references: 21835231
references: Theor Appl Genet. 2007 Dec;116(1):77-85
references: 17898985
references: Genetics. 2002 Dec;162(4):1937-43
references: 12524361
references: Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6478-83
references: 17420480
references: Theor Appl Genet. 2007 Oct;115(6):807-17
references: 17646960
references: PLoS One. 2014 Feb 27;9(2):e88804
references: 24586398
references: Theor Appl Genet. 2004 Aug;109(4):792-9
references: 15340689
references: FEBS J. 2009 Jul;276(13):3559-74
references: 19456863
references: Plant Cell Rep. 2006 Jun;25(6):592-8
references: 16432629
references: PLoS One. 2014 Mar 10;9(3):e91428
references: 24614913
references: Theor Appl Genet. 2009 Feb;118(3):413-21
references: 18979082
references: Bioinformatics. 2004 Sep 22;20(14):2324-6
references: 15059820
references: BMC Genomics. 2014 Feb 06;15:107
references: 24502855
references: New Phytol. 2012 Jan;193(1):96-108
references: 21973035
references: Plant Methods. 2008 May 20;4:10
references: 18492252
references: Theor Appl Genet. 1995 Feb;90(2):194-204
references: 24173891
references: Trends Plant Sci. 2010 May;15(5):283-90
references: 20303821
references: Plant J. 2004 Dec;40(6):893-908
references: 15584955
references: Crit Rev Food Sci Nutr. 1983;18(2):123-201
references: 6337782
references: Plant Physiol. 2001 Sep;127(1):108-18
references: 11553739
references: BMC Genomics. 2008 Mar 03;9:113
references: 18315867
references: BMC Genomics. 2014 May 23;15:396
references: 24886001
references: Annu Rev Plant Biol. 2006;57:303-33
references: 16669764
references: Genome. 2003 Jun;46(3):454-60
references: 12834062
references: Trends Plant Sci. 2002 Jun;7(6):263-70
references: 12049923