title: Myricitrin exhibits antioxidant, anti-inflammatory and antifibrotic activity in carbon tetrachloride-intoxicated mice.
abstract: Myricetin-3-O-α-rhamnoside (myricitrin) is a naturally occurring phenolic compound which possesses antioxidant and anti-inflammatory activity. The aim of this study was to determine the hepatoprotective effects of myricitrin. Myricitrin at doses of 10, 30 and 100 mg/kg and silymarin at dose of 100mg/kg were administered to BALB/cN mice by oral gavage, once daily for two consecutive days following carbon tetrachloride (CCl4)-intoxication. Myricitrin significantly ameliorated CCl4-induced increase in serum aspartate transaminase (AST) and alanine transaminase (ALT) levels and histopathological changes in the liver. Hepatic oxidative stress was reduced by myricitrin, as evidenced by the decrease in lipid peroxidation, with concomitant increase in glutathione (GSH) level and cytochrome P450 2E1 (CYP2E1) expression. In addition, cyclooxygenase-2 (COX-2) and tumor necrosis factor-alpha (TNF-α) overexpression in the liver was reduced, suggesting the suppression of inflammation. The expression of transforming growth factor-beta1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) was markedly ameliorated, indicating the inhibition of profibrotic response. Myricitrin also improved the regeneration of hepatic tissue after CCl4-intoxication, as evidenced by increased proliferating cell nuclear antigen (PCNA) expression. The results of the current study suggest that myricitrin exhibits a significant hepatoprotective activity. Myricitrin provided better hepatoprotection when compared to silymarin, which is consistent with its higher in vitro antioxidant potential.
authors: Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, Rijeka, Croatia. Electronic address: robert.domitrovic@medri.uniri.hr.
authors: Robert
authors: R
authors: Domitrović
authors: Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt.
authors: Khaled
authors: K
authors: Rashed
authors: Department of Anatomy, Medical Faculty, University of Rijeka, Rijeka, Croatia.
authors: Olga
authors: O
authors: Cvijanović
authors: Depatrment of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
authors: Sanda
authors: S
authors: Vladimir-Knežević
authors: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia.
authors: Marko
authors: M
authors: Škoda
authors: Požega General County Hospital, Pakrac, Croatia.
authors: Alenka
authors: A
authors: Višnić
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000517:prevention & control; D008297:Male; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D012838:Silymarin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D005419:Flavonoids; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; C008577:myricitrin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Antioxidant activity; Hepatic fibrosis; Hepatotoxicity; Inflammation; Myricetin-3-O-α-rhamnoside (myricitrin); Silymarin