title: Detection of antibodies against avian influenza virus by protein microarray using nucleoprotein expressed in insect cells.
abstract: Avian influenza (AI) is an infectious disease caused by avian influenza viruses (AIVs) which belong to the influenza virus A group. AI causes tremendous economic losses in poultry industry and pose great threatens to human health. Active serologic surveillance is necessary to prevent and control the spread of AI. In this study, a protein microarray using nucleoprotein (NP) of H5N1 AIV expressed in insect cells was developed to detect antibodies against AIV NP protein. The protein microarray was used to test Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), AIV positive and negative sera. The results indicated that the protein microarray could hybridize specifically with antibodies against AIV with strong signals and without cross-hybridization. Moreover, 76 field serum samples were detected by microarray, enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition test (HI). The positive rate was 92.1% (70/76), 93.4% (71/76) and 89.4% (68/76) by protein microarray, ELISA and HI test, respectively. Compared with ELISA, the microarray showed 100% (20/20) agreement ratio in chicken and 98.2% (55/56) in ornamental bird. In conclusion, this method provides an alternative serological diagnosis for influenza antibody screening and will provide a basis for the development of protein microarrays that can be used to respectively detect antibodies of different AIV subtypes and other pathogens.
authors: Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors: Yuhui
authors: Y
authors: Zhao
authors: Xiurong
authors: X
authors: Wang
authors: Pucheng
authors: P
authors: Chen
authors: Xianying
authors: X
authors: Zeng
authors: Hongmei
authors: H
authors: Bao
authors: Yunhe
authors: Y
authors: Wang
authors: Xiaolong
authors: X
authors: Xu
authors: Yongping
authors: Y
authors: Jiang
authors: Hualan
authors: H
authors: Chen
authors: Guangxing
authors: G
authors: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D015967:Gene Expression Regulation, Viral; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D009036:Moths; D009698:Nucleoproteins / Q000276:immunology* / Q000378:metabolism*; D040081:Protein Array Analysis / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D009698:Nucleoproteins
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