title: Challenges in DNA motion control and sequence readout using nanopore devices.
abstract: Nanopores are being hailed as a potential next-generation DNA sequencer that could provide cheap, high-throughput DNA analysis. In this review we present a detailed summary of the various sensing techniques being investigated for use in DNA sequencing and mapping applications. A crucial impasse to the success of nanopores as a reliable DNA analysis tool is the fast and stochastic nature of DNA translocation. We discuss the incorporation of biological motors to step DNA through a pore base-by-base, as well as the many experimental modifications attempted for the purpose of slowing and controlling DNA transport.
authors: Department of Physics, Northeastern University, Boston, MA 02115, USA.
authors: Spencer
authors: S
authors: Carson
authors: Meni
authors: M
authors: Wanunu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004563:Electrochemistry; D004581:Electronics; D004798:Enzymes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006460:Hemolysin Proteins / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D009038:Motion; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013269:Stochastic Processes
chemical_list: D004798:Enzymes; D006460:Hemolysin Proteins; D007477:Ions; D037742:Nanotubes, Carbon; D006108:Graphite; D004247:DNA
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