title: Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies.
abstract: Granulocyte immunofluorescence and granulocyte agglutination tests are standard methods for detecting human neutrophil antigen (HNA) antibodies (Abs); however, these require a typed panel of neutrophils, which can be time-consuming to develop, and it remains difficult to determine antibody specificity in some cases. We established and evaluated four detection systems for HNA-1a Abs based on an HNA-1a-expressing cell line (KY cells) and antigen capture. We additionally evaluated a commercial solid-phase system. Eleven HNA-1a antibody-positive samples, including the World Health Organization Reference Reagent, and 40 serum samples derived from male blood donors were used as positive and negative control samples, respectively. Although specificity was >0.90 in all systems evaluated, the sensitivity varied among the systems. The KY cell-based monoclonal antibody specific immobilisation of granulocyte antigens (KY-MAIGA) system using certain, but not all, monoclonal Abs, and the solid-phase system revealed higher sensitivity than other systems. In conclusion, the KY-MAIGA and commercial solid-phase systems were superior in terms of specific and sensitive detection of HNA-1a Abs.
authors: Japanese Red Cross Kinki Block Blood Center, 7-5-17, Saitoasagi, Ibaraki, Osaka, 567-0085, Japan.
authors: Etsuko
authors: E
authors: Amakishi
authors: Yoriko
authors: Y
authors: Irie
authors: Kanae
authors: K
authors: Nishizawa
authors: Hiromi
authors: H
authors: Kamada
authors: Fumiaki
authors: F
authors: Nakajima
authors: Nobuki
authors: N
authors: Matsuyama
authors: Hiroyuki
authors: H
authors: Ishii
authors: Harumichi
authors: H
authors: Matsukura
authors: Kazuta
authors: K
authors: Yasui
authors: Fumiya
authors: F
authors: Hirayama
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000097:blood / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D055767:Immobilized Proteins / Q000235:genetics / Q000276:immunology*; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins; D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human
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