title: Promotion of neural sprouting using low-level green light-emitting diode phototherapy.
abstract: We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW∕cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.
authors: Noa
authors: N
authors: Alon
authors: Hamootal
authors: H
authors: Duadi
authors: Ortal
authors: O
authors: Cohen
authors: Tamar
authors: T
authors: Samet
authors: Neta
authors: N
authors: Zilony
authors: Hadas
authors: H
authors: Schori
authors: Orit
authors: O
authors: Shefi
authors: Zeev
authors: Z
authors: Zalevsky
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D008027:Light; D008029:Lighting; D008954:Models, Biological; D016501:Neurites / Q000528:radiation effects*; D009474:Neurons / Q000528:radiation effects*; D010789:Phototherapy