title: Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents.
abstract: Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
authors: Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Katarina
authors: K
authors: Kluckova
authors: Lan-Feng
authors: LF
authors: Dong
authors: Martina
authors: M
authors: Bajzikova
authors: Jakub
authors: J
authors: Rohlena
authors: Jiri
authors: J
authors: Neuzil
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019069:Cell Respiration / Q000187:drug effects*; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species