title: Frequency of Epstein-Barr virus DNA sequences in human gliomas.
abstract: OBJECTIVE
  The Epstein-Barr virus (EBV) is the most common cause of infectious mononucleosis and is also associated with several human tumors, including Burkitt's lymphoma, Hodgkin's lymphoma, some cases of gastric carcinoma and nasopharyngeal carcinoma, among other neoplasms. The aim of this study was to screen 75 primary gliomas for the presence of specific EBV DNA sequences by means of the polymerase chain reaction (PCR), with confirmation by direct sequencing.
  
  
  METHODS
  Prevalence study on EBV molecular genetics at a molecular pathology laboratory in a university hospital and at an applied genetics laboratory in a national institution.
  
  
  METHODS
  A total of 75 primary glioma biopsies and 6 others from other tumors from the central nervous system were obtained. The tissues were immediately frozen for subsequent DNA extraction by means of traditional methods using proteinase K digestion and extraction with a phenol-chloroform-isoamyl alcohol mixture. DNA was precipitated with ethanol, resuspended in buffer and stored. The PCRs were carried out using primers for amplification of the EBV BamM region. Positive and negative controls were added to each reaction. The PCR products were used for direct sequencing for confirmation.
  
  
  RESULTS
  The viral sequences were positive in 11/75 (14.7%) of our samples.
  
  
  CONCLUSIONS
  The prevalence of EBV DNA was 11/75 (14.7%) in our glioma collection. Further molecular and epidemiological studies are needed to establish the possible role played by EBV in the tumorigenesis of gliomas.
authors: Congenital Malformations Laboratory, Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors: Renata Fragelli
authors: RF
authors: Fonseca
authors: Molecular Oncology Laboratory, Clementino Fraga Filho University Hospital.
authors: Siane Lopes Bittencourt
authors: SL
authors: Rosas
authors: Neurosurgery Service, Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil.
authors: José Antônio
authors: JA
authors: Oliveira
authors: Neurosurgery Service, Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil.
authors: Anselmo
authors: A
authors: Teixeira
authors: Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil.
authors: Gilda
authors: G
authors: Alves
authors: Molecular Pathology Laboratory, Pathology Department, Clementino Fraga Filho University Hospital.
authors: Maria da Glória Costa
authors: Mda G
authors: Carvalho
mesh_terms: D000293:Adolescent; D000328:Adult; D001254:Astrocytoma / Q000821:virology; D001932:Brain Neoplasms / Q000821:virology*; D002648:Child; D004279:DNA, Viral / Q000235:genetics*; D019286:Endopeptidase K; D005260:Female; D005910:Glioma / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004279:DNA, Viral; D019286:Endopeptidase K
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