title: Differential viral haemorrhagic septicaemia virus genotype IVb infection in fin fibroblast and epithelial cell lines from walleye, Sander vitreus (Mitchill), at cold temperatures.
abstract: A cell line, WE-cfin11e, with an epithelial-like morphology was developed from a caudal fin of walleye, Sander vitreus (Mitchill), characterized as distinct from the established walleye caudal fin fibroblast-like cell line, WE-cfin11f, and compared with WE-cfin11f for susceptibility to VHSV IVb. Immunocytochemistry and confocal microscopy were used to localize the intermediate filament protein, vimentin, the tight junction protein, zonula occludens-1 (ZO-1), the extracellular matrix protein, collagen I, and the viral protein, G. Although both cell lines contained vimentin, only WE-cfin11e stained for ZO-1 and only WE-cfin11f stained for collagen I. Ascorbic acid increased the accumulation of collagen I and caused the appearance of collagen fibres only in WE-cfin11f cultures. At 14 °C, both cell lines produced VHSV IVb, but the infection developed more rapidly in WE-cfin11f. At 4 °C, both cell lines became infected with VHSV IVb as judged by the expression of viral proteins, N and G, but only WE-cfin11f produced virus. The results suggest that cold temperatures can modulate viral tropism.
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: N T K
authors: NT
authors: Vo
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: A W
authors: AW
authors: Bender
authors: Ontario Veterinary College, Pathobiology, University of Guelph, Guelph, ON, Canada.
authors: J S
authors: JS
authors: Lumsden
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: B
authors: B
authors: Dixon
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: N C
authors: NC
authors: Bols
mesh_terms: D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D004847:Epithelial Cells / Q000821:virology; D005347:Fibroblasts / Q000821:virology; D005393:Fish Diseases / Q000821:virology*; D005399:Fishes; D005838:Genotype; D031941:Hemorrhagic Septicemia, Viral / Q000821:virology; D029141:Novirhabdovirus / Q000235:genetics / Q000502:physiology*; D010471:Perches; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: VHSV; caudal fin; epithelial cells; fibroblast; viral tropism; walleye