title: Human leukocyte antigen-G polymorphism influences the age of onset and autoantibody status in rheumatoid arthritis.
abstract: The study was conducted to investigate the frequency of three gene polymorphisms in the 3'-untranslated region (3'-UTR) of human leucocyte antigen-G (HLA-G) gene in south Indian patients with rheumatoid arthritis (RA) and analyze their influence on disease susceptibility, phenotype and treatment response. HLA-G 14 bp insertion (Ins)/deletion (del) (rs66554220), HLA-G +3142G>C (rs1063320) and +3187A>G (rs9380142) polymorphism was analyzed in 221 RA patients and 200 healthy controls. Frequency of HLA-G genotypes or alleles did not differ between patients and controls. Analysis based on rheumatoid factor (RF) status revealed that the frequency of allele 'A' (rs9380142) was significantly higher in RF-positive than in RF-negative patients [84% vs 74%, Yates-corrected P value (Pc) = 0.04, odds ratio (OR) = 1.8, 95% confidence interval (CI) = 1.0-3.2]. A similar difference was maintained in RF-positive female patients than their RF-negative counterparts (83% vs 71%, Pc = 0.02, OR = 1.9, 95% CI = 1.0 to 3.4) and between RF-positive and RF-negative young onset RA (YORA) patients (84% vs 73%, Pc = 0.03, OR = 1.9, 95% CI = 1.0-3.2), suggesting that rs9380142 polymorphism influenced RF status. The 14 bp Ins allele of rs66554220 was significantly more prevalent in RF-positive YORA than in RF-positive late onset RA (LORA) patients (51% vs 25%, P = 0.03, OR = 3.1, 95% CI = 1.1-9.8). Frequency of the four major haplotypes [InsGA (48%), DelGA (22%), DelCG (18%), DelCA (9.7%)] observed did not differ between cases and controls. HLA-G does not appear to be a risk factor for development of RA in south Indian Tamils but may act as a genetic modifier of clinical phenotype in terms of autoantibody production, gender preference and age at disease onset.
authors: Department of Clinical Immunology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India; INSERM, UMRS 1160, Saint Louis Hospital, Paris, France.
authors: C M
authors: CM
authors: Mariaselvam
authors: A B
authors: AB
authors: Chaaben
authors: S
authors: S
authors: Salah
authors: D
authors: D
authors: Charron
authors: R
authors: R
authors: Krishnamoorthy
authors: R
authors: R
authors: Tamouza
authors: V S
authors: VS
authors: Negi
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D017668:Age of Onset; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000453:epidemiology* / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D059951:HLA-G Antigens / Q000235:genetics* / Q000276:immunology; D006239:Haplotypes; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012217:Rheumatoid Factor / Q000235:genetics / Q000276:immunology; D012307:Risk Factors; D012737:Sex Factors
chemical_list: D020413:3' Untranslated Regions; D001323:Autoantibodies; D059951:HLA-G Antigens; D012217:Rheumatoid Factor
keywords: HLA G polymorphism; late onset rheumatoid arthritis; rheumatoid arthritis; rheumatoid factor; young onset rheumatoid arthritis