title: Molecular Detection and Identification of Theileria Species by PCR-RFLP Method in Sheep from Ahvaz, Southern Iran.
abstract: BACKGROUND
  The present study was carried out to investigate the accurate status of ovine Theileria infection in sheep from Ahvaz and surrounding region, a tropical area southwest Iran.
  
  
  METHODS
  A PCR-RFLP method based on 18S ribosomal RNA gene was designed which could detect and differentiate Theileria and Babesia spp. and also differentiate main Theileria species in sheep at the same time. 119 sheep blood samples were collected from Ahvaz and surroundings.
  
  
  RESULTS
  Microscopic examination of blood smears revealed 69.7% (83/119) infection with Theileria spp. Of the total samples subjected to PCR, 89% (106/119) were found to be positive, all of which were identified as Theileria by RFLP analysis using enzyme Hind II. In enzymatic digestion of PCR products by Vsp I, 91.5% (97/106) of Theileria positive samples were identified as T. ovis while mixed Theileria infections were found in 9 samples. The samples with mixed infections were analyzed with an additional nested PCR-RFLP method, by HpaII enzyme digestion. 3 samples with T. lestoquardi infection, 1 sample with T. ovis and T. annulata, 1 sample with T. lestoquardi and T. annulata, and 4 samples with T. ovis, T. lestoquardi and T. annulata mixed infections were detected.
  
  
  CONCLUSIONS
  Ovine theileriosis caused by T. ovis is highly prevalent in southwest Iran while T. lestoquardi and T. annulata infection can be detected in a lesser propor-tion of sheep in this region. The new PCR-RFLP method that was designed in this study, can serve as a beneficial diagnostic tool, especially in T. ovis prevalent re-gions.
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran ; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors: Seyedeh Missagh
authors: SM
authors: Jalali
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Zohreh
authors: Z
authors: Khaki
authors: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Bahram
authors: B
authors: Kazemi
authors: Parasitology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Sadegh
authors: S
authors: Rahbari
authors: Parasitology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Parviz
authors: P
authors: Shayan
authors: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Mojgan
authors: M
authors: Bandehpour
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: Seyedeh Parastoo
authors: SP
authors: Yasini
keywords: 18S rRNA gene; Iran; PCR-RFLP; Sheep; Theileria spp.Babesia
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