title: Development of a novel thermostable Newcastle disease virus vaccine vector for expression of a heterologous gene.
abstract: Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village poultry flocks, due to their independence of cold chains for delivery and storage. To explore the potential use of thermostable NDV as a vaccine vector, an infectious clone of thermostable avirulent NDV strain TS09-C was developed using reverse genetics technology. The GFP gene, along with the self-cleaving 2A gene of foot-and-mouth disease virus and ubiquitin monomer (2AUbi), were inserted immediately upstream of the NP (nucleocapsid protein), M (matrix protein) or L (large polymerase protein) gene translation start codon in the TS09-C infectious clone. Detection of GFP expression in the recombinant virus-infected cells showed that the recombinant virus, rTS-GFP/M, with the GFP gene inserted into the M gene expressed the highest level of GFP. The rTS-GFP/M virus retained the same thermostability, growth dynamics and pathogenicity as its parental rTS09-C virus. Vaccination of specific-pathogen-free chickens with the rTS-GFP/M virus conferred complete protection against virulent NDV challenge. Taken together, the data suggested that the rTS09-C virus could be used as a vaccine vector to develop bivalent thermostable vaccines against Newcastle disease and the target avian diseases for village chickens, especially in the developing and least-developed countries.
authors: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Wuhan 430070, PR China.|Southeast Poultry Research Laboratory, Agricultural Research Services, US Department of Agriculture, Athens, GA 30605, USA.|Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Guoyuan
authors: G
authors: Wen
authors: Veterinary Pathology Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.|Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Chen
authors: C
authors: Chen
authors: Veterinary Pathology Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.|Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Jing
authors: J
authors: Guo
authors: Southeast Poultry Research Laboratory, Agricultural Research Services, US Department of Agriculture, Athens, GA 30605, USA.
authors: Zhenyu
authors: Z
authors: Zhang
authors: Veterinary Pathology Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.|Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Yu
authors: Y
authors: Shang
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Huabin
authors: H
authors: Shao
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Qingping
authors: Q
authors: Luo
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Jun
authors: J
authors: Yang
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Hongling
authors: H
authors: Wang
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Hongcai
authors: H
authors: Wang
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Tengfei
authors: T
authors: Zhang
authors: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, PR China.
authors: Rongrong
authors: R
authors: Zhang
authors: Veterinary Pathology Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Guofu
authors: G
authors: Cheng
authors: Southeast Poultry Research Laboratory, Agricultural Research Services, US Department of Agriculture, Athens, GA 30605, USA.
authors: Qingzhong
authors: Q
authors: Yu
mesh_terms: D000818:Animals; D002645:Chickens; D004337:Drug Carriers; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D042822:Genomic Instability; D049452:Green Fluorescent Proteins / Q000235:genetics; D009521:Newcastle Disease / Q000517:prevention & control; D009522:Newcastle disease virus / Q000235:genetics*; D059386:Reverse Genetics; D016019:Survival Analysis; D025801:Ubiquitin / Q000235:genetics; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D004337:Drug Carriers; D025801:Ubiquitin; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C057421:virus protein 2A; D049452:Green Fluorescent Proteins