title: Time-resolved luminescent biosensing based on inorganic lanthanide-doped nanoprobes.
abstract: Time-resolved (TR) photoluminescence (PL) biosensing has been widely adopted in many research and medical institutions. However, commercial molecular TRPL bioprobes like lanthanide (Ln(3+))-chelates suffer from poor photochemical stability and long-term toxicity. Inorganic Ln(3+)-doped nanocrystals (NCs), owing to their superior physicochemical properties over Ln(3+)-chelates, are regarded as a new generation of luminescent nanoprobes for TRPL biosensing. The long-lived PL of Ln(3+)-doped NCs combined with the TRPL technique is able to completely suppress the interference of the short-lived background, resulting in a background-free signal and therefore a remarkable sensitivity for biosensing. In this feature article, we summarize the latest advancements in inorganic Ln(3+)-doped NCs as TRPL nano-bioprobes from their fundamental optical properties to their potential applications for ultrasensitive biodetection and high-resolution bioimaging. Future efforts towards the commercialization of these nanoprobes are also proposed.
authors: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China. xchen@fjirsm.ac.cn.
authors: Wei
authors: W
authors: Zheng
authors: Datao
authors: D
authors: Tu
authors: Ping
authors: P
authors: Huang
authors: Shanyong
authors: S
authors: Zhou
authors: Zhuo
authors: Z
authors: Chen
authors: Xueyuan
authors: X
authors: Chen
mesh_terms: D000818:Animals; D001001:Anura; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000097:blood; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D008027:Light; D049449:Luminescence; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D017430:Prostate-Specific Antigen / Q000097:blood; D015222:Sodium Channels / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D002272:Carcinoembryonic Antigen; D018997:Chorionic Gonadotropin, beta Subunit, Human; D028581:Lanthanoid Series Elements; D015222:Sodium Channels; D000509:alpha-Fetoproteins; D017430:Prostate-Specific Antigen