title: Identification of a novel stress regulated FERM domain containing cytosolic protein having PTP activity in Setaria cervi, a bovine filarial parasite.
abstract: A 67 kDa cytosolic FERM domain containing protein having significant protein tyrosine phosphatases activity (PTPL) has been purified to homogeneity from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 16 peptide peaks showing nearest match to Brugia malayi Moesin/ezrin/radixin homolog 1 protein and one peptide showing significant similarity with a region lying in the catalytic domain of human PTPD1. PTPL showed significant cross reactivity with the human PTP1B antibody and colocalize with actin in the coelomyrian cells of hypodermis in the parasite. PTPL was stress regulated as it showed marked decrease in the expression when exposed to Aspirin, an antifilarial drug and Phenylarsine Oxide, PTP inhibitor.
authors: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors: Neetu
authors: N
authors: Singh
authors: Third Faculty of Medicine, Charles University, Prague, Ruska 87, CZ-100 00 Prague 10, Czech Republic.
authors: Petr
authors: P
authors: Heneberg
authors: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors: Nidhi
authors: N
authors: Singh
authors: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors: Shio Kumar
authors: SK
authors: Singh
authors: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: surathaur@rediffmail.com.
authors: Sushma
authors: S
authors: Rathaur
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D020134:Catalytic Domain; D003429:Cross Reactions; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D017182:Setaria Nematode / Q000737:chemistry* / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D001152:Arsenicals; D015801:Helminth Proteins; C029341:oxophenylarsine; D017027:Protein Tyrosine Phosphatases; D001241:Aspirin
keywords: Cytoskeletal protein; FERM domain; Filariasis; Immunostaining; MALDI MS/MS; Tyrosine phosphatase