title: Bioactivity-guided fast screen and identification of cancer metastasis chemopreventive components from raw extracts of Murraya exotica.
abstract: Murraya exotica is a traditional Chinese medicine (TCM) widely grown in southeast China. We herein proposed a fast strategy for separation and identification of active components of cancer metastatic chemopreventives from the root, leaf, twig and stem bark extracts that were obtained by reflux in 80% acidic ethanol and then liquid-liquid extraction. High performance liquid chromatography (HPLC) analysis showed that the extract mixtures from leaf, bark and twig were similar, while the root extract contained a characteristic component (CM1). Bioactivity assays revealed that the root extract contained some active components that significantly inhibited cancer cell viability and migration. Ultra performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UPLC-DAD-ESI-MS) analysis indicated the existence of coumarins in the root and leaf extracts. Semi-preparative chromatographic separation and physicochemical characterization indicated that CM1 was a novel coumarin derivative that warrants further chemopreventive studies on cancer metastasis. The present phytochemical and phytopharmacological studies exemplify a fast strategy for screening and identifying active component(s) from raw extracts of phytomedicines.
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China.
authors: Zhou
authors: Z
authors: Jiang
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China.
authors: Jina
authors: J
authors: Yang
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China.
authors: Yaqiong
authors: Y
authors: Pang
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China.
authors: Xintian
authors: X
authors: Yang
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China. Electronic address: yshsmiling@126.com.
authors: Suhong
authors: S
authors: Yu
authors: Cancer Metastasis Alert and Prevention Center, and Bio-Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, Chemistry College, Fuzhou University, Fuzhou 350002, China. Electronic address: jiali@fzu.edu.cn.
authors: Lee
authors: L
authors: Jia
mesh_terms: D045744:Cell Line, Tumor; D018890:Chemoprevention / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods; D032101:Murraya / Q000737:chemistry*; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009369:Neoplasms / Q000188:drug therapy*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C030123:coumarin
keywords: Cancer metastasis chemoprevention; Coumarin; Liquid chromatography–mass spectrometry; Murraya exotica; Nuclear magnetic resonance