title: Antiosteoporotic activity and constituents of Podocarpium podocarpum.
abstract: Our study aimed to investigate the antiosteoporotic properties of the ethanol extract of Podocarpium podocarpum (DC.) Yang et Huang (PE) in ovariectomized (OVX) rats and to characterize the active constituents. As a result, PE significantly inhibited the increased urinary Ca excretion and activity of bone resorption markers including tartrate-resistant acid phosphatase (TRAP), deoxypyridinoline crosslinks and cathepsin K in OVX rats, whereas exhibited little effects on the body, uterus and vagina weight. Detailed micro-CT analysis showed that PE notably enhanced bone quality, with increased bone mineral content (BMC), bone volume fraction (BVF), connectivity density (CD), tissue mineral content (TMC), tissue mineral density (TMD) and trabecular number (Tb. N), and decreased trabecular separation (Tb. Sp), in OVX animal. Those findings implied that PE had notable antiosteoporotic effect, especially effective in preventing bone resorption, with little side-effects on reproductive tissue. Further chemical investigation led to the isolation of 17 flavonoids, most of which showed significantly stimulatory effect on osteoblastic proliferation, ALP activity and mineralized nodes formation as well as inhibitory effect on osteoclastic TRAP activity in osteoblastic and osteoclastic cells. Our results indicated that PE, with abundant flavonoids, had remarkable antiosteoporotic activity and therefore can be a promising candidate for the treatment of postmenopausal osteoporosis induced by estrogen deficiency through herbal remedy.
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China; Department of Biological Science, College of Life Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Fuzhou, 350002, China.
authors: Qi
authors: Q
authors: Ye
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China; Department of Pharmaceutical analysis, School of Pharmacy, NingXia Medical University, 1160 Shenli Street, Yinchuan, 750004, China.
authors: Xue-Qin
authors: XQ
authors: Ma
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China.
authors: Chang-Ling
authors: CL
authors: Hu
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China.
authors: Bing
authors: B
authors: Lin
authors: Department of Pharmaceutical analysis, School of Pharmacy, NingXia Medical University, 1160 Shenli Street, Yinchuan, 750004, China.
authors: Li-Sheng
authors: LS
authors: Xu
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China. Electronic address: zhengchengjian@smmu.edu.cn.
authors: Cheng-Jian
authors: CJ
authors: Zheng
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, 200433, PR China. Electronic address: qinsmmu@126.com.
authors: Lu-Ping
authors: LP
authors: Qin
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005269:Femur / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism; D015394:Molecular Structure; D010006:Osteoblasts / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D010024:Osteoporosis / Q000188:drug therapy*; D010052:Ovariectomy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D005419:Flavonoids; D007527:Isoenzymes; D010936:Plant Extracts; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Antiosteoporotic; Flavonoids; Osteoblast; Osteoclast; Podocarpium podocarpum