title: Genetic and antigenic characterization of Canadian invasive Neisseria meningitidis serogroup C (MenC) case isolates in the post-MenC conjugate vaccine era, 2009-2013.
abstract: We previously reported a shift in the electrophoretic type (ET) of invasive MenC in Canada from predominantly ET-15 to ET-37 in the post-MenC conjugate vaccine period. This study sought to confirm this trend by examining all culture-confirmed invasive MenC case isolates in Canada in the period from 1 January 2009 to 31 December 2013. Of the 50 MenC isolates, 18 belonged to ET-15, 28 belonged to ET-37 (but not ET-15), and four belonged to other clonal types. Analysis of the serotype and serosubtype antigens, porA and fetA gene sequences provided data to show that invasive MenC belonging to ET-15 and ET-37 were two very different subpopulations within the ST-11 clonal complex. Sequence analysis of the fHbp genes suggested that 12 different types of factor H-binding protein were found among the ET-15 isolates while 86 % of ET-37 isolates were found to have fHbp genes predicted to encode peptide 22. The nadA gene in 12 MenC isolates was disrupted due to IS1301 insertion and 11 of these 12 isolates belonged to ET-15. Ten per cent of the invasive MenC were found to have a frame-shift mutation in their fHbp genes that predicted no fHbp produced. Significant diversity and frame-shift mutations of fHbp genes were found in invasive MenC strains in Canada.
authors: Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada raymond.tsang@phac-aspc.gc.ca.
authors: Raymond S W
authors: RS
authors: Tsang
authors: BC Public Health Microbiology and Reference Laboratory, Vancouver, British Columbia, Canada.
authors: Linda
authors: L
authors: Hoang
authors: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Gregory
authors: G
authors: Tyrrell
authors: Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.
authors: Greg
authors: G
authors: Horsman
authors: Cadham Provincial Public Health Laboratory, Winnipeg, Manitoba, Canada.
authors: John
authors: J
authors: Wylie
authors: Public Health Ontario Laboratory, Public Health Ontario, Ontario, Canada Faculty of Medicine, University of Toronto, Ontario, Canada.
authors: Frances B
authors: FB
authors: Jamieson
authors: Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.
authors: Brigitte
authors: B
authors: Lefebvre
authors: Institut Pasteur, Invasive Bacterial Infections Unit and National Reference Centre for Meningococci, Paris, France.
authors: Muhamed-Kheir
authors: MK
authors: Taha
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics; D002170:Canada / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008585:Meningitis, Meningococcal / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control*; D022401:Meningococcal Vaccines / Q000008:administration & dosage* / Q000276:immunology; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D038542:Neisseria meningitidis, Serogroup C / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018272:Porins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D065288:Serogroup; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C093120:FrpB protein, bacteria; D022401:Meningococcal Vaccines; D018272:Porins; C484699:factor H-binding protein, Neisseria meningitidis; C057699:porin protein, Neisseria; C410218:serogroup C meningococcal conjugate vaccine