title: Complex formation of RNA silencing proteins in the perinuclear region of Neurospora crassa.
abstract: In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly.
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Logan M
authors: LM
authors: Decker
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Erin C
authors: EC
authors: Boone
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Hua
authors: H
authors: Xiao
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Benjamin S
authors: BS
authors: Shanker
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Shannon F
authors: SF
authors: Boone
authors: Department of Biology, Barry University, Miami Shores, Florida 33161.
authors: Shanika L
authors: SL
authors: Kingston
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
authors: Seung A
authors: SA
authors: Lee
authors: School of Biological Sciences, Illinois State University, Normal, Illinois, 61790.
authors: Thomas M
authors: TM
authors: Hammond
authors: Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 shiup@missouri.edu.
authors: Patrick K T
authors: PK
authors: Shiu
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism; D009492:Neurospora crassa / Q000235:genetics*; D011485:Protein Binding; D021381:Protein Transport; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D005656:Fungal Proteins; D028884:Karyopherins; D043244:Ribonuclease III
keywords: Neurospora crassa; RNA interference; bimolecular fluorescence complementation (BiFC); fluorescent protein tagging; meiotic silencing by unpaired DNA (MSUD)
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