title: Heat-transfer-method-based cell culture quality assay through cell detection by surface imprinted polymers.
abstract: Previous work has indicated that surface imprinted polymers (SIPs) allow for highly specific cell detection through macromolecular cell imprints. The combination of SIPs with a heat-transfer-based read-out technique has led to the development of a selective, label-free, low-cost, and user-friendly cell detection assay. In this study, the breast cancer cell line ZR-75-1 is used to assess the potential of the platform for monitoring the quality of a cell culture in time. For this purpose, we show that the proposed methodology is able to discriminate between the original cell line (adherent growth, ZR-75-1a) and a descendant cell line (suspension growth, ZR-75-1s). Moreover, ZR-75-1a cells were cultured for a prolonged period of time and analyzed using the heat-transfer method (HTM) at regular time intervals. The results of these experiments demonstrate that the thermal resistance (Rth) signal decays after a certain number of cell culture passages. This can likely be attributed to a compromised quality of the cell culture due to cross-contamination with the ZR-75-1s cell line, a finding that was confirmed by classical STR DNA profiling. The cells do not express the same functional groups on their membrane, resulting in a weaker bond between cell and imprint, enabling cell removal by mechanical friction, provided by flushing the measuring chamber with buffer solution. These findings were further confirmed by HTM and illustrate that the biomimetic sensor platform can be used as an assay for monitoring the quality of cell cultures in time.
authors: Hasselt University , Institute for Materials Research IMO, Wetenschapspark 1, Diepenbeek, Belgium.
authors: Kasper
authors: K
authors: Eersels
authors: Bart
authors: B
authors: van Grinsven
authors: Mehran
authors: M
authors: Khorshid
authors: Veerle
authors: V
authors: Somers
authors: Christiane
authors: C
authors: Püttmann
authors: Christoph
authors: C
authors: Stein
authors: Stefan
authors: S
authors: Barth
authors: Hanne
authors: H
authors: Diliën
authors: Gerard M J
authors: GM
authors: Bos
authors: Wilfred T V
authors: WT
authors: Germeraad
authors: Thomas J
authors: TJ
authors: Cleij
authors: Ronald
authors: R
authors: Thoelen
authors: Ward
authors: W
authors: De Ceuninck
authors: Patrick
authors: P
authors: Wagner
mesh_terms: D032701:Biomimetics / Q000379:methods*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D006358:Hot Temperature; D006801:Humans; D018895:Microsatellite Repeats; D054802:Molecular Imprinting; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis* / Q000378:metabolism; D011786:Quality Control; D013499:Surface Properties
chemical_list: D015335:Molecular Probes; D011108:Polymers