title: The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution.
abstract: Lead (Pb), as a heavy metal element, has become the most important metal pollutant of the environment. With allocating a relatively higher proportion of its biomass in roots, maize could be a potential important model to study the phytoremediation of Pb-contaminated soil. Here we analyzed the maize root transcriptome of inbred lines 9782 under heavy metal lead (Pb) pollution, which was identified as a non-hyperaccumulator for Pb in roots. In the present study, more than 98 millions reads were mapped to define gene structure and detect polymorphism, thereby to qualify transcript abundance along roots development under Pb treatment. A total of 17,707, 17,440, 16,998 and 16,586 genes were identified in maize roots at four developmental stages (0, 12 h, 24 h and 48 h) respectively and 2,825, 2,626, 2161 and 2260 stage-specifically expressed genes were also identified respectively. In addition, based on our RNA-Seq data, transcriptomic changes during maize root development responsive to Pb were investigated. A total of 384 differentially expressed genes (DEGs) (log2Ratio ≥ 1, FDR ≤ 0.001) were identified, of which, 36 genes with significant alteration in expression were detected in four developmental stages; 12 DEGs were randomly selected and successful validated by qRT-PCR. Additionally, many transcription factor families might act as the important regulators at different developmental stages, such as bZIP, ERF and GARP et al. These results will expand our understanding of the complex molecular and cellular events in maize root development and provide a foundation for future study on root development in maize under heavy metal pollution and other cereal crops.
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Jian
authors: J
authors: Gao
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Yongzhong
authors: Y
authors: Zhang
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Chaolong
authors: C
authors: Lu
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China; Sichuan Tourism University, Chengdu, 610000, Sichuan, China.
authors: Hua
authors: H
authors: Peng
authors: Drug Discovery Research Center of Luzhou Medical College, Luzhou, Sichuan 646000, China.
authors: Mao
authors: M
authors: Luo
authors: Guangdong Academy of Agriculture Sciences, Gongzhou, 510640, China.
authors: Gaoke
authors: G
authors: Li
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Yaou
authors: Y
authors: Shen
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Haiping
authors: H
authors: Ding
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
authors: Zhiming
authors: Z
authors: Zhang
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China. Electronic address: pangt1956@aliyun.com.
authors: Guangtang
authors: G
authors: Pan
authors: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China. Electronic address: linhj521@gmail.com.
authors: Haijian
authors: H
authors: Lin
mesh_terms: D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D007854:Lead / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology*; D012989:Soil Pollutants / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects / Q000502:physiology*; D003313:Zea mays / Q000187:drug effects / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D012989:Soil Pollutants; D007854:Lead
keywords: Differential expression genes (DEGs); Pb pollution; RNAseq; qRT-PCR