title: Selective room temperature phosphorescence detection of heparin based on manganese-doped zinc sulfide quantum dots/polybrene self-assembled nanosensor.
abstract: A selective system was developed to detect heparin in aqueous solutions by using MPA(3-Mercaptopropionic Acid)-capped Mn-doped ZnS quantum dots (QDs)/polybrene (hexadimethrine bromide) hybrids as a sensitive room temperature phosphorescence (RTP) nanosensor. In this system, the RTP intensity of QDs was remarkably enhanced via electrostatic self-assembly after the addition of polybrene. The addition of heparin into the system was competitively bound to polybrene and enable to deprive it from the surface of QDs, as a result, the RTP intensity of Mn-doped ZnS QDs/polybrene hybrids was reduced with the increased of heparin concentration. Based on this effect, a selective system was proposed to detect heparin. Under the optimal conditions, the change of RTP intensity was proportional to the heparin concentration from 0.05 to 1.4 U mL(-1) (about 0.38-10.76 μg mL(-1)) and the limit of detection (LOD) was 0.021 U mL(-1) (about 0.16 μg mL(-1)). This proposed nanosensor is simple and relatively free of interference from coexisting substances, which can be applied to detect heparin in heparin injection and human serum. In addition, a new pathway was also provided based on the assembly of QDs with other cationic homopolymers for further design of biosensors and detection of biomolecules.
authors: Shanxi Normal University, Linfen, Shanxi 041000, China.
authors: Zhifeng
authors: Z
authors: Zhang
authors: Shanxi Normal University, Linfen, Shanxi 041000, China.
authors: Yanming
authors: Y
authors: Miao
authors: Shanxi Normal University, Linfen, Shanxi 041000, China.
authors: Qindi
authors: Q
authors: Zhang
authors: Shanxi Normal University, Linfen, Shanxi 041000, China.
authors: Linwang
authors: L
authors: Lian
authors: Shanxi Normal University, Linfen, Shanxi 041000, China. Electronic address: gqyan@126.com.
authors: Guiqin
authors: G
authors: Yan
mesh_terms: D015374:Biosensing Techniques; D006493:Heparin / Q000737:chemistry / Q000302:isolation & purification*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D006801:Humans; D008163:Luminescent Measurements; D008345:Manganese / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D045663:Quantum Dots; D013440:Sulfides / Q000737:chemistry; D013696:Temperature; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D006583:Hexadimethrine Bromide; D006493:Heparin; C031238:zinc sulfide
keywords: Detection; Heparin; Polybrene; Room-temperature phosphorescence (RTP); Self-assembly