title: Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains.
abstract: The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein-protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes.
authors: Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Dragomir
authors: D
authors: Milovanovic
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Alf
authors: A
authors: Honigmann
authors: Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Seiichi
authors: S
authors: Koike
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Fabian
authors: F
authors: Göttfert
authors: Institute for Physical Chemistry, Georg-August-University, Göttingen D-37077, Germany.
authors: Gesa
authors: G
authors: Pähler
authors: Institute for Organic and Biomolecular Chemistry, Georg-August-University, Göttingen D-37077, Germany.
authors: Meike
authors: M
authors: Junius
authors: Institute for Organic and Biomolecular Chemistry, Georg-August-University, Göttingen D-37077, Germany.
authors: Stefan
authors: S
authors: Müllar
authors: Institute for Organic and Biomolecular Chemistry, Georg-August-University, Göttingen D-37077, Germany.
authors: Ulf
authors: U
authors: Diederichsen
authors: Institute for Physical Chemistry, Georg-August-University, Göttingen D-37077, Germany.
authors: Andreas
authors: A
authors: Janshoff
authors: Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Helmut
authors: H
authors: Grubmüller
authors: 1] Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany [2] Department of Chemistry, Leibnitz Institute of Surface Modification, D-04318 Leipzig, Germany.
authors: Herre J
authors: HJ
authors: Risselada
authors: 1] Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany [2] MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.
authors: Christian
authors: C
authors: Eggeling
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Stefan W
authors: SW
authors: Hell
authors: 1] Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany [2] Department of Tumor Immunology, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.
authors: Geert
authors: G
authors: van den Bogaart
authors: Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
authors: Reinhard
authors: R
authors: Jahn
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer; D005455:Fluorescent Antibody Technique; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D056004:Molecular Dynamics Simulation; D010716:Phosphatidylinositols / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D050600:SNARE Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D010716:Phosphatidylinositols; D050600:SNARE Proteins
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