title: Rapid publication-ready MS-Word tables for two-way ANOVA.
abstract: BACKGROUND
  Statistical tables are an essential component of scientific papers and reports in biomedical and agricultural sciences. Measurements in these tables are summarized as mean ± SEM for each treatment group. Results from pairwise-comparison tests are often included using letter displays, in which treatment means that are not significantly different, are followed by a common letter. However, the traditional manual processes for computation and presentation of statistically significant outcomes in MS Word tables using a letter-based algorithm are tedious and prone to errors.
  
  
  RESULTS
  Using the R package 'Shiny', we present a web-based program freely available online, at https://houssein-assaad.shinyapps.io/TwoWayANOVA/. No download is required. The program is capable of rapidly generating publication-ready tables containing two-way analysis of variance (ANOVA) results. Additionally, the software can perform multiple comparisons of means using the Duncan, Student-Newman-Keuls, Tukey Kramer, Westfall, and Fisher's least significant difference (LSD) tests. If the LSD test is selected, multiple methods (e.g., Bonferroni and Holm) are available for adjusting p-values. Significance statements resulting from all pairwise comparisons are included in the table using the popular letter display algorithm. With the application of our software, the procedures of ANOVA can be completed within seconds using a web-browser, preferably Mozilla Firefox or Google Chrome, and a few mouse clicks. To our awareness, none of the currently available commercial (e.g., Stata, SPSS and SAS) or open-source software (e.g., R and Python) can perform such a rapid task without advanced knowledge of the corresponding programming language.
  
  
  CONCLUSIONS
  The new and user-friendly program described in this paper should help scientists perform statistical analysis and rapidly generate publication-ready MS-Word tables for two-way ANOVA. Our software is expected to facilitate research in agriculture, biomedicine, and other fields of life sciences.
authors: Department of Statistics, Texas A & M University, College Station, TX 77843 USA ; Department of Animal Science, Texas A & M University, College Station, TX 77843 USA.
authors: Houssein I
authors: HI
authors: Assaad
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023 China.
authors: Yongqing
authors: Y
authors: Hou
authors: Department of Statistics, Texas A & M University, College Station, TX 77843 USA.
authors: Lan
authors: L
authors: Zhou
authors: Department of Statistics, Texas A & M University, College Station, TX 77843 USA.
authors: Raymond J
authors: RJ
authors: Carroll
authors: Department of Animal Science, Texas A & M University, College Station, TX 77843 USA ; Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023 China.
authors: Guoyao
authors: G
authors: Wu
keywords: Agriculture; Biology; Multiple comparisons; Online software; R; Shiny; Statistical analysis; Two-way ANOVA
references: Am J Physiol. 1997 Jun;272(6 Pt 1):G1382-90
references: 9227473
references: FASEB J. 2014 Jul;28(7):2852-63
references: 24627544
references: Front Biosci (Landmark Ed). 2015 Jan 01;20:814-28
references: 25553481
references: J Nutr. 2009 Feb;139(2):230-7
references: 19106310
references: Springerplus. 2014 Aug 27;3:474
references: 25191639
references: Amino Acids. 2012 May;42(5):2031-5
references: 21547361
references: Front Biosci (Landmark Ed). 2015 Jan 01;20:872-91
references: 25553484
references: Biom J. 2008 Jun;50(3):346-63
references: 18481363
references: J Nutr. 2015 Jan;145(1):25-31
references: 25527658
references: Amino Acids. 2013 Mar;44(3):911-23
references: 23117836
references: Amino Acids. 2009 May;37(1):187-98
references: 19212806
references: J Pharmacol Exp Ther. 1997 Apr;281(1):448-53
references: 9103529
references: Front Biosci (Landmark Ed). 2014 Jun 01;19:967-85
references: 24896330
references: Methods Enzymol. 2008;440:177-89
references: 18423217
references: Amino Acids. 2014 Aug;46(8):2037-45
references: 24858859
references: Diabetes. 1995 Mar;44(3):360-4
references: 7533735