title: Induction of the lateral flagellar system of Vibrio shilonii is an early event after inhibition of the sodium ion flux in the polar flagellum.
abstract: In this study, we show the induction of lateral flagella by the action of the sodium channel blocker phenamil, in the marine bacterium Vibrio shilonii, a coral pathogen that causes bleaching. We analyzed the growth and morphology of cells treated with phenamil. A time course analysis showed that after 30 min of exposure to the sodium channel blocker, lateral flagella were present and could be detected by electron microscopy. Detection of the mRNA of the master regulator (lafK) and lateral flagellin (lafA) by RT-PCR confirmed the expression of lateral flagellar genes. We show the simultaneous isolation of polar and, for the first time, lateral flagellar hook-basal bodies. This allowed us to compare the dimensions and morphological characteristics of the 2 structures.
authors: Instituto de Fisiología Celular, Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México. Ap. Postal 70-243, Cd. Universitaria, México DF 04510, Mexico.
authors: Yael
authors: Y
authors: González
authors: Laura
authors: L
authors: Camarena
authors: Georges
authors: G
authors: Dreyfus
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D005407:Flagella / Q000235:genetics / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D014733:Vibrio / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin; D012964:Sodium
keywords: Vibrio shilonii; bacterial motility; flagelles activés par le sodium; flagelles latéraux activés par les protons; motilité bactérienne; proton-driven lateral flagella; sodium-driven flagella