title: Anti-inflammatory effect of resveratrol through the suppression of NF-κB and JAK/STAT signaling pathways.
abstract: Resveratrol, the most important ingredient extracted from Polygonum cuspidatum, exerts cytoprotective effects via anti-inflammatory actions in vitro. In this study, we investigated this effect of resveratrol on the lipopolysaccharide (LPS)-induced inflammatory response and its underlying molecular mechanism of action in RAW264.7 murine macrophages. Results showed that resveratrol down-regulated the expression of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6), therefore, suppressed the production of nitric oxide and the secretion of IL-6 in LPS-stimulated RAW264.7 cells in a dose-dependent manner. Resveratrol also inhibited the translocation of high-mobility group box 1 (HMGB1) from the nucleus to the cytoplasm and of nuclear transcription factor kappa-B (NF-κB) p65 from the cytoplasm to the nucleus; it suppressed the phosphorylation of IκBα. Furthermore, these actions were mediated by suppressing the phosphorylation of signal transducer and activator of transcription (STAT)-1 and -3. In conclusion, these data indicate that resveratrol exerts anti-inflammatory effects, at least in part by reducing the release of HMGB1 and modulating the NF-κB and Janus kinase/STAT signaling pathways. Resveratrol could potentially be developed as a useful agent for the chemoprevention of inflammatory diseases.
authors: The Second Afﬁliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China.
authors: Chunfang
authors: C
authors: Ma
authors: The Second Afﬁliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China.
authors: Yin
authors: Y
authors: Wang
authors: The Second Afﬁliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China.
authors: Lei
authors: L
authors: Dong
authors: The Second Afﬁliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China.
authors: Minjing
authors: M
authors: Li
authors: The Second Afﬁliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China sundaymore@126.com.
authors: Wanru
authors: W
authors: Cai
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D053612:Janus Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D000067996:RAW 264.7 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D020672:I-kappa B Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; C498144:Rela protein, mouse; D050791:STAT Transcription Factors; D013267:Stilbenes; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D053612:Janus Kinases; D000077185:Resveratrol
keywords: JAK/STAT; NF-κB; anti-inflammatory; high-mobility group box 1; resveratrol