title: MicroRNAs let-7b/i suppress human glioma cell invasion and migration by targeting IKBKE directly.
abstract: We demonstrated that IKBKE is overexpressed in human gliomas and that the downregulation of IKBKE markedly inhibits the proliferative and invasive abilities of glioma cells, which is consistent with the results reported by several different research groups. Therefore, IKBKE represents a promising therapeutic target for the treatment of glioma. In the present study, we verified that the microRNAs let-7b and let-7i target IKBKE through luciferase assays and found that let-7b/i mimics can knock down IKBKE and upregulate E-cadherin through western blot analysis. Moreover, the expression levels of let-7b/i were significantly lower in glioma cell lines than that in normal brain tissues, as determined by quantitative real-time PCR. Furthermore, let-7b/i inhibit the invasion and migration of glioma cells, as determined through wound healing and Transwell assays. The above-mentioned data suggest that let-7b/i inhibit the invasive ability of glioma cells by directly downregulating IKBKE and indirectly upregulating E-cadherin.
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Yuan
authors: Y
authors: Tian
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Shaobo
authors: S
authors: Hao
authors: Department of Neurosurgery, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, People's Republic of China.
authors: Minhua
authors: M
authors: Ye
authors: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Anling
authors: A
authors: Zhang
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China.
authors: Yang
authors: Y
authors: Nan
authors: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Guangxiu
authors: G
authors: Wang
authors: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Zhifan
authors: Z
authors: Jia
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China.
authors: Kai
authors: K
authors: Yu
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China.
authors: Lianmei
authors: L
authors: Guo
authors: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, People's Republic of China; Key Laboratory of Neurotrauma, Variation and Regeneration, Ministry of Education and Tianjin Municipal Government, People's Republic of China.
authors: Peiyu
authors: P
authors: Pu
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China. Electronic address: huangqiang209@163.com.
authors: Qiang
authors: Q
authors: Huang
authors: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China. Electronic address: zhongyue2457@sina.com.
authors: Yue
authors: Y
authors: Zhong
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D051550:I-kappa B Kinase / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology
chemical_list: D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D051550:I-kappa B Kinase; C496565:IKBKE protein, human
keywords: E-cadherin; Glioma; IKBKE; Let-7b/i