title: Attached cultivation for improving the biomass productivity of Spirulina platensis.
abstract: To improve cultivation efficiency for microalgae Spirulina platensis is related to increase its potential use as food source and as an effective alternative for CO2 fixation. The present work attempted to establish a technique, namely attached cultivation, for S. platensis. Laboratory experiments were made firstly to investigate optimal conditions on attached cultivation. The optimal conditions were found: 25 g m(-2) for initial inoculum density using electrostatic flocking cloth as substrata, light intensity lower than 200 μmol m(-2) s(-1), CO2 enriched air flow (0.5%) at a superficial aeration rate of 0.0056 m s(-1) in a NaHCO3-free Zarrouk medium. An outdoor attached cultivation bench-scale bioreactor was built and a 10d culture of S. platensis was carried out with daily harvesting. A high footprint areal biomass productivity of 60 g m(-2) d(-1) was obtained. The nutrition of S. platensis with attached cultivation is identical to that with conventional liquid cultivation.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266101, PR China; Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors: Lanlan
authors: L
authors: Zhang
authors: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors: Lin
authors: L
authors: Chen
authors: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors: Junfeng
authors: J
authors: Wang
authors: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors: Yu
authors: Y
authors: Chen
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266101, PR China.
authors: Xin
authors: X
authors: Gao
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266101, PR China. Electronic address: zhangzhh@ouc.edu.cn.
authors: Zhaohui
authors: Z
authors: Zhang
authors: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China. Electronic address: liutz@qibebt.ac.cn.
authors: Tianzhong
authors: T
authors: Liu
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D020418:Algal Proteins / Q000032:analysis; D018441:Biofilms; D018533:Biomass; D019149:Bioreactors; D002241:Carbohydrates / Q000032:analysis; D002245:Carbon Dioxide / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D008055:Lipids / Q000032:analysis; D017785:Photons; D053545:Spirulina / Q000187:drug effects / Q000254:growth & development*
chemical_list: D020418:Algal Proteins; D002241:Carbohydrates; D008055:Lipids; D002245:Carbon Dioxide
keywords: Attached cultivation; Biomass productivity; Outdoor bench-scale cultivation; Spirulina platensis