title: HPV genotyping among women treated for high-grade cervical intraepithelial neoplasia with no lesion in the conization specimen.
abstract: OBJECTIVE
  To evaluate whether specific HPV genotypes or multiple HPV infection are associated with absence of cervical intraepithelial neoplasia (CIN) in the conization specimen.
  
  
  METHODS
  In a retrospective study, data were reviewed for women treated by conization at a center in Barcelona, Spain, between 2008 and 2011. Women whose pretreatment biopsy showed CIN2/3 with positive p16 staining but had no lesions in the conization specimen were included in the study group if material was sufficient for HPV genotyping. Age-matched control patients who had CIN2/3 in the conization specimen were selected. HPV genotyping was conducted on all histologic specimens.
  
  
  RESULTS
  Both groups contained 43 patients. High-risk HPV genotypes were identified in the conization specimens of 14 (33%) women in the study group and of 42 (98%) in the control group (P<0.001). HPV16/HPV18 was detected in the pretreatment biopsy samples of 27 (63%) women in the study group and 25 (58%) in the control group (P=0.413). Multiple HPV infections were detected pretreatment in 8 (19%) women in the study group and 9 (21%) in the control group (P=0.50).
  
  
  CONCLUSIONS
  No association was found between HPV genotype or multiple HPV infection and absence of lesion in the conization specimen.
authors: Institute Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain.
authors: Ágata
authors: Á
authors: Rodríguez-Manfredi
authors: DDL Diagnostic Laboratory, Rijswijk, Netherlands.
authors: Romy
authors: R
authors: van Baars
authors: DDL Diagnostic Laboratory, Rijswijk, Netherlands.
authors: Wim G V
authors: WG
authors: Quint
authors: Institute Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain.
authors: Ma Jesús
authors: MJ
authors: Sanchez
authors: Institute Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain.
authors: Aureli
authors: A
authors: Torné
authors: Department of Pathology, Centre de Recerca en Salut Internacional de Barcelona, Hospital Clínic, University of Barcelona, Barcelona, Spain.
authors: Jaume
authors: J
authors: Ordi
authors: Institute Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain. Electronic address: mdelpino@clinic.ub.es.
authors: Marta
authors: M
authors: Del Pino
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019092:Conization; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology; D012189:Retrospective Studies; D013030:Spain; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology*
keywords: Cervical intraepithelial neoplasia; HPV genotyping; Histologic negative conization; Loop electrosurgical excision procedure (LEEP)