title: Epidermal growth factor improves the migration and contractility of aged fibroblasts cultured on 3D collagen matrices.
abstract: Epidermal growth factor (EGF) plays a critical role in fibroblasts by stimulating the production of collagen and supports cell renewal through the interaction between keratinocytes and fibroblasts. It is well known that the contractile activity of fibroblasts is required for the remodeling of the extracellular matrix (ECM), which contributes to skin elasticity. However, the role of EGF in the contraction of aged fibroblasts under 3-dimensional (3D) culture conditions is not yet fully understood. In the present study, we demonstrated that young fibroblasts spread and proliferated more rapidly than aged fibroblasts under 2-dimensional (2D) culture conditions. Cell migration assay using a nested collagen matrix revealed that the migration of young fibroblasts was also greater than that of aged fibroblasts under 3D culture conditions. However, the addition of recombinant human EGF (rhEGF) resulted in the enhanced migration of aged fibroblasts; the migration rate was similar to that of the young fibroblasts. The aged fibroblasts showed decreased cluster formation compared with the young fibroblasts on the collagen matrix, which was improved by the addition of rhEGF. Furthermore, cell contraction assay revealed that the basal contractility of the aged fibroblasts was lower than that of the young fibroblasts; however, following treatment with rhEGF, the contractility was restored to levels similar or even higher to those of the young fibroblasts. Taken together, our results suggest that rhEGF is a potential renewal agent that acts to improve the migration and contraction of aged fibroblasts more efficiently than young fibroblasts under 3D culture conditions; thus, EGF may have valuable regenerative effects on aged skin.
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul, Republic of Korea.
authors: Daehwan
authors: D
authors: Kim
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.
authors: So Young
authors: SY
authors: Kim
authors: Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors: Seog Kyun
authors: SK
authors: Mun
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul, Republic of Korea.
authors: Sangmyung
authors: S
authors: Rhee
authors: Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.
authors: Beom Joon
authors: BJ
authors: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects*; D016922:Cellular Senescence; D002648:Child; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D012867:Skin / Q000166:cytology / Q000378:metabolism; D055815:Young Adult
chemical_list: D004815:Epidermal Growth Factor