title: The Bacteroides thetaiotaomicron protein Bacteroides host factor A participates in integration of the integrative conjugative element CTnDOT into the chromosome.
abstract: CTnDOT is a conjugative transposon found in Bacteroides species. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroides host factor A), that participates in integrative recombination. BHFa is the first host factor identified for a site-specific recombination reaction in the CTnDOT family of integrative and conjugative elements. Based on the amino acid sequence of BHFa, the ability to bind specifically to 4 sites in the attDOT DNA, and its activity in the integration reaction, BHFa is a member of the IHF/HU family of nucleoid-associated proteins. Other DNA bending proteins that bind DNA nonspecifically can substitute for BHFa in the integration reaction.
  
  
  OBJECTIVE
  Bacteroides species are normal members of the human colonic microbiota. These species can harbor and spread self-transmissible genetic elements (integrative conjugative elements [ICEs]) that contain antibiotic resistance genes. This work describes the role of a protein, BHFa, and its importance in the integration reaction required for the element CTnDOT to persist in Bacteroides host cells.
authors: Department of Microbiology, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors: Kenneth
authors: K
authors: Ringwald
authors: Department of Microbiology, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA jeffgard@life.illinois.edu.
authors: Jeffrey
authors: J
authors: Gardner
mesh_terms: D001439:Bacteroides / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D018983:DNA Footprinting; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D034982:Integration Host Factors / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D034982:Integration Host Factors
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