title: Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels.
abstract: Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required for its growth on hemicellulose. Reconstitution of this xylodextrin utilization pathway in Saccharomyces cerevisiae revealed that fungal xylose reductases act as xylodextrin reductases, producing xylosyl-xylitol oligomers as metabolic intermediates. These xylosyl-xylitol intermediates are generated by diverse fungi and bacteria, indicating that xylodextrin reduction is widespread in nature. Xylodextrins and xylosyl-xylitol oligomers are then hydrolyzed by two hydrolases to generate intracellular xylose and xylitol. Xylodextrin consumption using a xylodextrin transporter, xylodextrin reductases and tandem intracellular hydrolases in cofermentations with sucrose and glucose greatly expands the capacity of yeast to use plant cell wall-derived sugars and has the potential to increase the efficiency of both first-generation and next-generation biofuel production.
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Xin
authors: X
authors: Li
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Vivian Yaci
authors: VY
authors: Yu
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Yuping
authors: Y
authors: Lin
authors: Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
authors: Kulika
authors: K
authors: Chomvong
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Raíssa
authors: R
authors: Estrela
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Annsea
authors: A
authors: Park
authors: Department of Chemistry, University of California, Berkeley, Berkeley, United States.
authors: Julie M
authors: JM
authors: Liang
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Elizabeth A
authors: EA
authors: Znameroski
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Joanna
authors: J
authors: Feehan
authors: Institute for Genomic Biology, University of Illinois, Urbana, United States.
authors: Soo Rin
authors: SR
authors: Kim
authors: Institute for Genomic Biology, University of Illinois, Urbana, United States.
authors: Yong-Su
authors: YS
authors: Jin
authors: Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
authors: N Louise
authors: NL
authors: Glass
authors: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors: Jamie H D
authors: JH
authors: Cate
mesh_terms: D056804:Biofuels; D002473:Cell Wall / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D056804:Biofuels; D014994:Xylose
keywords: B. subtilis; E. coli; N. crassa; S. cerevisiae; biofuel; cofermentation; computational biology; ecology; hemicellulose; systems biology; xylodextrin; xylose; xylosyl-xylitol
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