title: Identification of urinary protein biomarkers for tobacco smoking.
abstract: Smoking, passive smoking, and nonsmoking are conditions that give different degrees of stress to the body. In this study, a proteomic technique was used to analyze differentially urinary protein expression between these three groups of subjects. Urinary proteins were precipitated using ammonium sulfate followed by separation according to molecular weights using SDS-PAGE. The gel was stained by Coommassie blue, and the image of the gel was captured for the comparison study. The protein bands that were consistently detected but expressed at different intensity between the smokers and nonsmokers were targeted for further analysis. Three targeted protein bands were excised from the gel, consisting of a unique protein band of smokers and a pair of differentially expressed protein bands from smokers and nonsmokers. The proteins were digested in gel by trypsin. The tryptic peptides were analyzed with ultra performance liquid chromatography-tandem mass spectrometry. Protein identity was determined by the product ion spectrum in the MS/MS scan. Four unique proteins from the smokers, namely, pancreatic alpha amylase, proepidermal growth factor, protein 4.1, and prostatic acid phosphatase, were found to be potential urinary biomarkers to indicate smoking status of a person.
authors: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors: Aj Nabill
authors: AN
authors: Haniff
authors: Doping Control Centre, Universiti Sains Malaysia, Penang, Malaysia.|School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors: Lay-Harn
authors: LH
authors: Gam
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000652:urine; D020374:Chromatography, Micellar Electrokinetic Capillary; D006801:Humans; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012907:Smoking / Q000652:urine*; D053719:Tandem Mass Spectrometry; D014556:Urine / Q000737:chemistry*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D011506:Proteins
keywords: biomarkers; mass spectrometry; proteomics; tobacco smoking