title: Enriching hematopoietic, endothelial and mesenchymal functional progenitors by short-term culture of steady-state peripheral blood mononuclear cells obtained from healthy donors and ischemic patients.
abstract: BACKGROUND
  Non-mobilized peripheral blood contains mostly committed cells with limited numbers of early progenitors.
  
  
  OBJECTIVE
  To enrich functional progenitor cells from healthy donors and ischemic heart disease patients by short-term culture of mononuclear cells with defined culture conditions.
  
  
  METHODS
  Mononuclear cells obtained from healthy donors and ischemic heart disease patients were cultured for7 days in a cytokine cocktail. We tested the multilineage differentiation capacities and phenotype of cultured cells.
  
  
  RESULTS
  The short-term culture (7 days) of all study groups with a defined cytokine cocktail resulted in two distinct cell populations (adherent and non-adherent) that differed in their differentiation capacities as well as their cell surface markers. Cultured adherent cells showed higher differentiation potential and expressed endothelial and mesenchymal fibroblast-like surface markers as compared to fresh non-cultured mononuclear cells. The non-adherent cell fraction demonstrated high numbers of colony-forming units, indicating a higher differentiation potential of hematopoietic lineage.
  
  
  CONCLUSIONS
  This study proved the feasibility of increasing limited numbers of multipotent progenitor cells obtained from the non-mobilized peripheral blood of healthy donors and ischemic patients. Moreover, we found that each of the two enriched subpopulations (adherent and non-adherent) has a different differentiation potential (mesenchymal, endothelial and hematopoietic).
authors: Nira
authors: N
authors: Varda-Bloom
authors: Avraham J
authors: AJ
authors: Treves
authors: Tatiana
authors: T
authors: Kroupnik
authors: Dan
authors: D
authors: Spiegelstein
authors: Ehud
authors: E
authors: Raanani
authors: Arnon
authors: A
authors: Nagler
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000378:metabolism; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000378:metabolism; D066026:Endothelial Progenitor Cells / Q000502:physiology*; D005240:Feasibility Studies; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood*; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D008565:Membrane Proteins