title: Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus.
abstract: The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.
authors: MARE-Marine and Environmental Sciences Centre, ISPA-Instituto Universitário , Rua Jardim do Tabaco, Lisbon , Portugal.
authors: Carla
authors: C
authors: Sousa-Santos
authors: Departamento de Biologia Animal and Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa , Campo Grande, Lisbon , Portugal.
authors: Paulo J
authors: PJ
authors: Fonseca
authors: MARE-Marine and Environmental Sciences Centre, ISPA-Instituto Universitário , Rua Jardim do Tabaco, Lisbon , Portugal.
authors: Maria Clara P
authors: MC
authors: Amorim
keywords: Batrachoididae; Microsatellite development; Paternity; Polymorphic loci; Pyrosequencing
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