title: Higher levels of mucosal antibody to pneumococcal vaccine candidate proteins are associated with reduced acute otitis media caused by Streptococcus pneumoniae in young children.
abstract: Mucosal immunity has a crucial role in controlling human respiratory tract infections. This study characterizes the naturally acquired mucosal antibody levels to three Streptococcus pneumoniae (Spn) protein antigens, pneumococcal histidine triad protein D (PhtD), pneumococcal choline binding protein A (PcpA), and pneumolysin (Ply), and assesses the association of the mucosal antibody levels with occurrence of acute otitis media (AOM) caused by Spn. Both nasopharyngeal (NP) immunoglobulin G (IgG) and IgA levels to all three proteins slightly decreased in children from 6 to 9 months of age and then gradually increased through 24 months of age. Spn NP colonization was associated with higher mucosal antibody levels to all three proteins. However, children with Spn AOM had 5-8-fold lower IgG and 3-6-fold lower IgA levels to the three proteins than children without AOM but asymptomatically colonized with Spn. Antigen-specific antibody levels in the middle ear fluid (MEF) were correlated with antibody levels in the NP. Children with AOM caused by Spn had lower antibody levels in both the MEF and NP than children with AOM caused by other pathogens. These results indicate that higher naturally acquired mucosal antibody levels to PhtD, PcpA and Ply are associated with reduced AOM caused by Spn.
authors: Rochester General Hospital Research Institute, Rochester, New York, USA.
authors: Q
authors: Q
authors: Xu
authors: Legacy Pediatrics, Rochester, New York, USA.
authors: J R
authors: JR
authors: Casey
authors: Rochester General Hospital Research Institute, Rochester, New York, USA.
authors: M E
authors: ME
authors: Pichichero
mesh_terms: D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004432:Ear, Middle / Q000276:immunology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007223:Infant; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology*; D010033:Otitis Media / Q000276:immunology / Q000517:prevention & control; D011008:Pneumococcal Infections / Q000276:immunology / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D022242:Pneumococcal Vaccines
references: Vaccine. 2003 Oct 1;21(27-30):4328-34
references: 14505915
references: Clin Vaccine Immunol. 2012 Oct;19(10):1593-6
references: 22855395
references: Vaccine. 2006 Jan 9;24(1):57-65
references: 16115703
references: JAMA. 2007 Oct 17;298(15):1772-8
references: 17940232
references: Vaccine. 2012 Dec 14;30(52):7461-8
references: 23123106
references: J Clin Microbiol. 2011 Nov;49(11):3750-5
references: 21900518
references: Vaccine. 2010 Jul 12;28(31):5012-6
references: 20546832
references: Microbes Infect. 2012 Oct;14(12):1102-10
references: 22796387
references: Vaccine. 2012 Dec 14;30(52):7455-60
references: 23131206
references: Vaccine. 2014 May 30;32(26):3205-10
references: 24731814
references: Infect Immun. 2012 Jun;80(6):2186-93
references: 22451514
references: Crit Rev Microbiol. 2015 Jun;41(2):190-200
references: 23895377
references: J Infect Dis. 2001 Aug 15;184(4):451-9
references: 11471103
references: Clin Microbiol Infect. 2010 Aug;16(8):1312-7
references: 19832714
references: J Virol. 1988 Jul;62(7):2234-7
references: 3373569
references: PLoS Pathog. 2013 Jan;9(1):e1003057
references: 23326226
references: Infect Immun. 2008 Jun;76(6):2767-76
references: 18391008
references: Curr Opin Virol. 2012 Jun;2(3):225-32
references: 22542216
references: Vaccine. 2014 Feb 3;32(6):706-11
references: 24355091
references: Vaccine. 2007 Apr 20;25(16):2963-73
references: 17324490
references: Hum Vaccin Immunother. 2012 Jun;8(6):799-805
references: 22495112
references: Vaccine. 2009 Jul 23;27(34):4615-21
references: 19524618
references: Ann N Y Acad Sci. 2007 Mar;1098:288-311
references: 17435136
references: BMC Infect Dis. 2010;10:73
references: 20298596
references: J Infect. 2014 Oct;69(4):309-25
references: 24968238
references: Multidiscip Respir Med. 2013 Jul 18;8(1):46
references: 23866900
references: Trends Microbiol. 2010 Feb;18(2):81-9
references: 20031415
references: PLoS One. 2013;8(10):e78916
references: 24205351
references: Clin Vaccine Immunol. 2014 Aug;21(8):1037-45
references: 24850621
references: Clin Microbiol Rev. 2003 Apr;16(2):230-41
references: 12692096
references: Curr Opin Pulm Med. 2010 May;16(3):217-25
references: 20375783
references: Curr Infect Dis Rep. 2006 May;8(3):189-95
references: 16643770
references: J Clin Virol. 2010 Aug;48(4):239-45
references: 20646956
references: J Biol Chem. 2012 Aug 10;287(33):27885-94
references: 22736767
references: PLoS One. 2013;8(11):e81168
references: 24312273
references: Vaccine. 2010 Oct 18;28(44):7184-92
references: 20800701
references: Vaccine. 2009 Dec 9;27(52):7359-66
references: 19800447
references: BMC Infect Dis. 2013;13:70
references: 23384407
references: Pediatrics. 2009 Jun;123(6):e1103-10
references: 19482744
references: J Infect Dis. 2014 Apr 1;209(7):1116-25
references: 24041791
references: Curr Opin Immunol. 2011 Jun;23(3):407-13
references: 21514128
references: Vaccine. 2013 Jan 2;31(2):327-33
references: 23153437
references: Pediatrics. 2010 Mar;125(3):429-36
references: 20176669