title: Stereochemistry of enzymatic water addition to C=C bonds.
abstract: Water addition to carbon-carbon double bonds using hydratases is attracting great interest in biochemistry. Most of the known hydratases are involved in primary metabolism and to a lesser extent in secondary metabolism. New hydratases have recently been added to the toolbox, both from natural sources or artificial metalloenzymes. In order to comprehensively understand how the hydratases are able to catalyse the water addition to carbon-carbon double bonds, this review will highlight the mechanistic and stereochemical studies of the enzymatic water addition to carbon-carbon double bonds, focusing on the syn/anti-addition and stereochemistry of the reaction.
authors: Biokatalyse, Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands.
authors: Bi-Shuang
authors: BS
authors: Chen
authors: Biokatalyse, Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands.
authors: Linda G
authors: LG
authors: Otten
authors: Biokatalyse, Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands. Electronic address: u.hanefeld@tudelft.nl.
authors: Ulf
authors: U
authors: Hanefeld
mesh_terms: D001671:Biochemistry; D002244:Carbon; D002384:Catalysis; D006836:Hydro-Lyases / Q000378:metabolism*; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014867:Water; D002244:Carbon; D006836:Hydro-Lyases
keywords: Alcohol; Anti-addition; Hydratase; Hydro-lyase; Michael addition; Stereochemistry; Syn-addition; Water addition