title: Antioxidant Potentials and Xanthine Oxidase Inhibitory Effect of Two Furanocoumarins Isolated from Tamus communis L.
abstract: In this investigation, the screening of two furanocoumarins; 5,8- dimethoxypsoralen (1) and heraclinin (2), isolated from the methanol root-extracts of Tamus communis L for their antioxidant activity and xanthine oxidase inhibitory effect was carried out, using different assays such as DPPH free radical scavenging effect, β- carotene / linoleic acid, xanthine oxidase (XO) inhibition and in addition to blood total antioxidant capacity. Results revealed that the two compounds have significant DPPH radical scavenging activity and effective inhibition of linoleic acid oxidation in a dose-dependent manner; 5,8-dimethoxypsoralen exhibited the highest activity with an I% = 72.69 ± 1.88%. These results indicate that the isolated compounds inhibit xanthine oxidase activity and scavenge superoxide radicals with heraclinin (2) as the more potent xanthine oxidase inhibitor, and 5,8-dimethoxypsoralen (1) as the more effective on cytochrome c reduction, the two tested compounds can effectively protect erythrocytes against hemolytic injury induced by AAPH. These results are promising for further studies of the biological and pathological effects of these natural products.
authors: Fatima
authors: F
authors: Zerargui
authors: Sabah
authors: S
authors: Boumerfeg
authors: Noureddine
authors: N
authors: Charef
authors: Abderrahmane
authors: A
authors: Baghiani
authors: Meriem
authors: M
authors: Djarmouni
authors: Seddik
authors: S
authors: Khennouf
authors: Lekhmici
authors: L
authors: Arrar
authors: Musa H
authors: MH
authors: Abu Zarga
authors: Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif 19000, Algeria and Department of Chemistry, Faculty of Science, The University of Jordan, Amman 11942, Jordan. mmubarak@ju.edu.jo.
authors: Mohammad S
authors: MS
authors: Mubarak
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011564:Furocoumarins / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D027744:Tamus / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D011564:Furocoumarins; D010936:Plant Extracts; C000606733:heraclinin; C015304:isopimpinellin; D014969:Xanthine Oxidase