title: Toxicity of the anti-ribosomal Lectin Ebulin f in lungs and intestines in elderly mice.
abstract: All parts of dwarf elder (Sambucus ebulus L.) studied so far contain a ribosome-inactivating protein with lectin activity (ribosome-inactivating lectin; RIL), known as ebulin. Green fruits contain ebulin f, the toxicity of which has been studied in six-week-old mice, where it was found that the intestines were primary targets for it when administered intraperitoneally (i.p.). We performed experiments to assess whether ebulin f administration to six- and 12-month-old mice would trigger higher toxicity than that displayed in six-week-old mice. In the present report, we present evidence indicating that the toxicological effects of ebulin f after its i.p. administration to elderly mice are exerted on the lungs and intestines by an increased rate of apoptosis. We hypothesize that the ebulin f apoptosis-promoting action together with the age-dependent high rate of apoptosis result in an increase in the lectin's toxicity, leading to a higher lethality level.
authors: Cell Biology, Histology and Pharmacology, Faculty of Medicine and INCYL (Institute of Neurosciences of Castile and Leon), University of Valladolid, Valladolid E-47005, Spain. gayoso@med.uva.es.
authors: Manuel
authors: M
authors: Garrosa
authors: Nutrition and Food Science, Faculty of Medicine, University of Valladolid, Valladolid E-47005, Spain. pilarj@bio.uva.es.|CINAD (Center for Research in Nutrition, Food and Dietetics), University of Valladolid, Valladolid E-47005, Spain. pilarj@bio.uva.es.
authors: Pilar
authors: P
authors: Jiménez
authors: Nutrition and Food Science, Faculty of Medicine, University of Valladolid, Valladolid E-47005, Spain. jesus.tejero@uva.es.|CINAD (Center for Research in Nutrition, Food and Dietetics), University of Valladolid, Valladolid E-47005, Spain. jesus.tejero@uva.es.
authors: Jesús
authors: J
authors: Tejero
authors: Nutrition and Food Science, Faculty of Medicine, University of Valladolid, Valladolid E-47005, Spain. patricia_cabrero@hotmail.com.
authors: Patricia
authors: P
authors: Cabrero
authors: Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy and IUFI (Institute of Industrial Pharmacy), Complutense University of Madrid, Madrid E-28040, Spain. damianco@farm.ucm.es.
authors: Damián
authors: D
authors: Cordoba-Diaz
authors: Nutrition and Food Science, Faculty of Medicine, University of Valladolid, Valladolid E-47005, Spain. equinto@ped.uva.es.|CINAD (Center for Research in Nutrition, Food and Dietetics), University of Valladolid, Valladolid E-47005, Spain. equinto@ped.uva.es.
authors: Emiliano J
authors: EJ
authors: Quinto
authors: Cell Biology, Histology and Pharmacology, Faculty of Medicine and INCYL (Institute of Neurosciences of Castile and Leon), University of Valladolid, Valladolid E-47005, Spain. gayoso@med.uva.es.
authors: Manuel J
authors: MJ
authors: Gayoso
authors: Nutrition and Food Science, Faculty of Medicine, University of Valladolid, Valladolid E-47005, Spain. girbes@bio.uva.es.|CINAD (Center for Research in Nutrition, Food and Dietetics), University of Valladolid, Valladolid E-47005, Spain. girbes@bio.uva.es.
authors: Tomás
authors: T
authors: Girbés
mesh_terms: D000375:Aging / Q000187:drug effects / Q000473:pathology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005638:Fruit / Q000737:chemistry; D007274:Injections, Intraperitoneal; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D053208:Kaplan-Meier Estimate; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D054790:Ribosome Inactivating Proteins, Type 2 / Q000302:isolation & purification / Q000633:toxicity*; D027923:Sambucus / Q000737:chemistry
chemical_list: D054790:Ribosome Inactivating Proteins, Type 2; C581455:ebulin f, Sambucus ebulus
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