title: Dynamic changes in the spatio-temporal expression of the β-defensin SPAG11C in the developing rat epididymis and its regulation by androgens.
abstract: Herein, we characterized the spatio-temporal expression, cellular distribution and regulation by androgens of the β-defensin SPAG11C, the rat ortholog of the human SPAG11B isoform C, in the developing epididymis by using RT-PCR, in situ hybridization and immunohistochemistry. We observed that Spag11c mRNA was ubiquitously expressed in rat fetuses, but preferentially detected in male reproductive tissues at adulthood. SPAG11C (mRNA and protein) was prenatally mainly detected in the mesenchyme of the Wolffian duct, switching gradually after birth to a predominant localization in the epididymis epithelium during postnatal development. In the adult epididymis, smooth muscle and interstitial cells were also identified as sources of SPAG11C. Furthermore, SPAG11C was differentially immunolocalized on spermatozoa surface during their transit from testis throughout caput and cauda epididymis. Developmental and surgical castration studies suggested that androgens contribute to the epididymal cell type- and region-specific modulation of SPAG11C mRNA levels and immunolocalization. Together our findings provide novel insights into the potential role of β-defensins in the epididymis.
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Camilla M
authors: CM
authors: Ribeiro
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Daniel B C
authors: DB
authors: Queiróz
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Marília T C C
authors: MT
authors: Patrão
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Alexandre
authors: A
authors: Denadai-Souza
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Renata M
authors: RM
authors: Romano
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
authors: Erick J R
authors: EJ
authors: Silva
authors: Section of Experimental Endocrinology, Department of Pharmacology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil. Electronic address: avellar@unifesp.br.
authors: Maria Christina W
authors: MC
authors: Avellar
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000378:metabolism; D004822:Epididymis / Q000254:growth & development* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism; D009919:Orchiectomy; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014928:Wolffian Ducts / Q000378:metabolism*; D023083:beta-Defensins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000728:Androgens; C487040:Spag11a protein, rat; D023083:beta-Defensins
keywords: Androgens; Development; Epididymis; Male fertility; SPAG11B; β-Defensins