title: ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo.
abstract: Abscission is the final step of cytokinesis that involves the cleavage of the intercellular bridge connecting the two daughter cells. Recent studies have given novel insight into the spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast and human cells. The mechanisms of abscission in living metazoan tissues are however not well understood. Here we show that ALIX and the ESCRT-III component Shrub are required for completion of abscission during Drosophila female germline stem cell (fGSC) division. Loss of ALIX or Shrub function in fGSCs leads to delayed abscission and the consequent formation of stem cysts in which chains of daughter cells remain interconnected to the fGSC via midbody rings and fusome. We demonstrate that ALIX and Shrub interact and that they co-localize at midbody rings and midbodies during cytokinetic abscission in fGSCs. Mechanistically, we show that the direct interaction between ALIX and Shrub is required to ensure cytokinesis completion with normal kinetics in fGSCs. We conclude that ALIX and ESCRT-III coordinately control abscission in Drosophila fGSCs and that their complex formation is required for accurate abscission timing in GSCs in vivo.
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Åsmund H
authors: ÅH
authors: Eikenes
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Lene
authors: L
authors: Malerød
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Anette Lie
authors: AL
authors: Christensen
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Chloé B
authors: CB
authors: Steen
authors: Department of Genetics and Developmental Biology, Institut Curie, Paris, France; CNRS UMR3215, Inserm U934 F-75248, Paris, France.
authors: Juliette
authors: J
authors: Mathieu
authors: School of Life Sciences, University of Warwick, Coventry, United Kingdom.
authors: Ioannis P
authors: IP
authors: Nezis
authors: Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway; Department of Informatics, University of Oslo, Oslo, Norway.
authors: Knut
authors: K
authors: Liestøl
authors: Department of Genetics and Developmental Biology, Institut Curie, Paris, France; CNRS UMR3215, Inserm U934 F-75248, Paris, France.
authors: Jean-René
authors: JR
authors: Huynh
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Harald
authors: H
authors: Stenmark
authors: Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway; Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Kaisa
authors: K
authors: Haglund
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D048749:Cytokinesis / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism; D011992:Endosomes / Q000235:genetics; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D060066:Protein Interaction Maps / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: C511870:ALIX protein, Drosophila; D029721:Drosophila Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C513148:Shrb protein, Drosophila
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