title: Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
abstract: We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
authors: Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Yuseong-gu, Daejeon, South Korea.
authors: Daesub
authors: D
authors: Song
authors: BioNote, Inc., Hwaseong-si, Gyeonggi-do, South Korea.
authors: Gunwoo
authors: G
authors: Ha
authors: Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Wissam
authors: W
authors: Serhan
authors: Epidemiology Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Yassir
authors: Y
authors: Eltahir
authors: Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Mohammed
authors: M
authors: Yusof
authors: Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Farouq
authors: F
authors: Hashem
authors: Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Elsaeid
authors: E
authors: Elsayed
authors: Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Bahaaeldin
authors: B
authors: Marzoug
authors: Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.
authors: Assem
authors: A
authors: Abdelazim
authors: Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates salama.almuhairi@adfca.ae.
authors: Salama
authors: S
authors: Al Muhairi
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002162:Camelus / Q000821:virology*; D002846:Chromatography, Affinity / Q000379:methods*; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000662:veterinary / Q000821:virology; D065207:Middle East Respiratory Syndrome Coronavirus / Q000302:isolation & purification*; D009296:Nasal Cavity / Q000821:virology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014773:Virology
chemical_list: D000956:Antigens, Viral
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