title: Bioactive Polyphenols from the Methanol Extract of Cnicus arvensis (L.) Roth Demonstrated Antinociceptive and Central Nervous System Depressant Activities in Mice.
abstract: Cnicus arvensis is used by many ethnic groups for inflammation, pain, and other ailments. In this study, reducing sugar, carbohydrate, alkaloid, steroid, tannin, flavonoid, and saponin groups were identified using standard chromogenic method. In high-performance liquid chromatography, vanillic acid and epicatechin were identified in the extract. Antinociceptive test by acetic acid induced writhing inhibition resulted 43.17 and 95.08% inhibition for 100 and 200 mg/kg body weight, comparing with standard diclofenac Na with 74.86% inhibition for 25 mg/kg body weight. In formalin induced paw licking test for antinociceptive activity, the extract inhibited 69.87 and 75.55% licking for 150 and 300 mg/kg body weight comparing with the inhibition (68.56%) of diclofenac Na for 10 mg/kg body weight at first phase. At late phase, the extract showed 73.12 and 87.46% licking comparing with licking inhibition (71.69%) by diclofenac Na at the same dose. In open field test for CNS depressant activity, the extract showed depression of locomotor activity for 150 and 300 mg/kg body weight comparing with diazepam for 10 mg/kg body weight. All results were statistically significant (P < 0.01). The identified polyphenols are reputed for antinociceptive and CNS depressant activity. The present findings support the use of this plant in pain.
authors: Natural Product Chemistry and Pharmacology Research Laboratory, Faculty of Health Sciences, Department of Pharmacy, Northern University Bangladesh, Dhaka 1205, Bangladesh.
authors: Mahmudur
authors: 0000-0003-2408-3958
authors: M
authors: Rahman
authors: Phytochemistry and Pharmacology Research Laboratory, Department of Pharmacy, Manarat International University, Dhaka 1216, Bangladesh.
authors: Amina
authors: 0000-0002-5631-8574
authors: A
authors: Khatun
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
authors: Mst Luthfun
authors: ML
authors: Nesa
authors: Chemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific & Industrial Research, Dhaka 1205, Bangladesh.
authors: Hemayet
authors: 0000-0001-8759-9279
authors: H
authors: Hossain
authors: Chemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific & Industrial Research, Dhaka 1205, Bangladesh.
authors: Ismet Ara
authors: IA
authors: Jahan
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