title: Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5.
abstract: BACKGROUND
  Clostridium difficile strain 630Δerm is a spontaneous erythromycin sensitive derivative of the reference strain 630 obtained by serial passaging in antibiotic-free media. It is widely used as a defined and tractable C. difficile strain. Though largely similar to the ancestral strain, it demonstrates phenotypic differences that might be the result of underlying genetic changes. Here, we performed a de novo assembly based on single-molecule real-time sequencing and an analysis of major methylation patterns.
  
  
  RESULTS
  In addition to single nucleotide polymorphisms and various indels, we found that the mobile element CTn5 is present in the gene encoding the methyltransferase rumA rather than adhesin CD1844 where it is located in the reference strain.
  
  
  CONCLUSIONS
  Together, the genetic features identified in this study may help to explain at least part of the phenotypic differences. The annotated genome sequence of this lab strain, including the first analysis of major methylation patterns, will be a valuable resource for genetic research on C. difficile.
authors: Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. e.van_eijk@lumc.nl.
authors: Erika
authors: E
authors: van Eijk
authors: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands. S.Y.Anvar@lumc.nl.|Leiden Genome Technology Center, Human and Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands. S.Y.Anvar@lumc.nl.
authors: Seyed Yahya
authors: SY
authors: Anvar
authors: Wellcome Trust Sanger Institute, Cambridge, Hinxton, UK. hb4@sanger.ac.uk.
authors: Hilary P
authors: HP
authors: Browne
authors: Sequence Analysis Support Core, Leiden University Medical Center, Leiden, the Netherlands. W.Y.Leung@lumc.nl.
authors: Wai Yi
authors: WY
authors: Leung
authors: Leiden Genome Technology Center, Human and Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands. J.Frank@lumc.nl.
authors: Jeroen
authors: J
authors: Frank
authors: Leiden Genome Technology Center, Human and Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands. A.M.Schmitz@lumc.nl.
authors: Arnoud M
authors: AM
authors: Schmitz
authors: Department of Microbial Diseases, UCL Eastman Dental Institute, London, UK. adam.roberts@ucl.ac.uk.
authors: Adam P
authors: AP
authors: Roberts
authors: Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. w.k.smits@lumc.nl.
authors: Wiep Klaas
authors: WK
authors: Smits
mesh_terms: D001483:Base Sequence; D016360:Clostridioides difficile / Q000235:genetics*; D019175:DNA Methylation / Q000187:drug effects; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004761:Enterocolitis, Pseudomembranous / Q000188:drug therapy / Q000235:genetics* / Q000382:microbiology; D004917:Erythromycin / Q000627:therapeutic use; D016680:Genome, Bacterial; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D004917:Erythromycin
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