title: Effect of curcumin on rat sperm morphology after the freeze-thawing process.
abstract: Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-thawing process. Sperm collected from 10 adult rats were divided into two groups (n=10 for each group): control and a test group supplemented with 2.5 mM curcumin. After freezing-thawing, the number of spermatozoa, motility, viability, total antioxidant capacity (TAC), and DNA integrity of the sperm were analyzed. Motility, viability and DNA integrity of sperm were significantly preserved in treatment groups compared to the control (p < 0.05) after freezing-thawing. Following cryopreservation, TAC was significantly preserved in thawing semen supplemented with curcumin compared to the control group (p<0.05). Based on our results, it is concluded that curcumin addition during freezing resulted in positive effects on sperm parameters after thawing in adult rats.
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Ali
authors: A
authors: Soleimanzadeh
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Adel
authors: A
authors: Saberivand
keywords: Curcumin; Rat; Sperm parameters
references: Hum Reprod. 2009 Jul;24(7):1717-25
references: 19279034
references: Prague Med Rep. 2011;112(4):288-97
references: 22142524
references: Cell Tissue Bank. 2006;7(2):91-8
references: 16732411
references: Fertil Steril. 2009 Jan;91(1):271-7
references: 18222428
references: Theriogenology. 2011 Apr 15;75(7):1319-26
references: 21295825
references: J Atheroscler Thromb. 2002;9(5):243-50
references: 12409634
references: Free Radic Biol Med. 1998 Jan 1;24(1):49-54
references: 9436613
references: Theriogenology. 2012 Oct 15;78(7):1548-56
references: 22925635
references: Zhonghua Nan Ke Xue. 2006 Aug;12(8):703-5
references: 16970158
references: Biol Reprod. 1989 Jul;41(1):183-97
references: 2553141
references: Theriogenology. 2003 Apr 15;59(8):1693-705
references: 12566145
references: Zhonghua Nan Ke Xue. 2004 Mar;10(3):222-6
references: 15080074
references: Biochem Pharmacol. 2011 Nov 1;82(9):1073-8
references: 21787756
references: Cancer Lett. 2005 Jun 8;223(2):181-90
references: 15896452
references: Anticancer Res. 2001 Jul-Aug;21(4B):2895-900
references: 11712783
references: Anim Reprod Sci. 2010 Jan;117(1-2):34-42
references: 19481887
references: Acta Pol Pharm. 2007 Sep-Oct;64(5):413-6
references: 18540160
references: Andrologia. 2001 Mar;33(2):79-86
references: 11350371
references: Front Biosci. 1996 Aug 01;1:e78-86
references: 9159248
references: Basic Clin Pharmacol Toxicol. 2007 Jan;100(1):43-8
references: 17214610
references: Eur J Obstet Gynecol Reprod Biol. 2004 Sep 10;116(1):63-6
references: 15294370
references: Pharmacol Toxicol. 2003 Jan;92(1):33-8
references: 12710595
references: Biochim Biophys Acta. 1994 Nov 10;1224(2):255-63
references: 7981240
references: Fertil Steril. 1984 Jul;42(1):87-91
references: 6724015
references: J Agric Food Chem. 2001 May;49(5):2539-47
references: 11368633
references: Food Chem Toxicol. 2009 Jan;47(1):249-54
references: 19049818
references: Ann N Y Acad Sci. 2005 Jun;1043:440-51
references: 16037265
references: Chem Biol Interact. 2008 May 28;173(2):105-14
references: 18377885
references: Fertil Steril. 2008 Sep;90(3):775-80
references: 18163997
references: Anal Biochem. 1996 Jul 15;239(1):70-6
references: 8660627
references: Eur J Cancer. 2005 Sep;41(13):1955-68
references: 16081279
references: Anim Reprod Sci. 2000 Aug 18;62(1-3):143-72
references: 10924823
references: Environ Toxicol Pharmacol. 2007 Sep;24(2):160-6
references: 21783805
references: Int J Androl. 1997 Apr;20(2):61-9
references: 9292315
references: Theriogenology. 2001 Jul 15;56(2):275-86
references: 11480619