title: Nonfouling tunable βCD dextran polymer films for protein applications.
abstract: Polymeric β-cyclodextrin (βCD) films tunable with respect to thickness and βCD content were prepared in order to develop a suitable platform, allowing for inclusion of nonpolar guest molecules in the βCD cavity, while suppressing nonspecific protein adsorption. The βCD films were synthesized from linear βCD dextran polymers, and grafted onto silicon oxide surfaces by "click" chemistry. Topographic and morphological characteristics are controllable by reaction conditions and polymer type, with average film heights from 2.5 to 12.5 nm. Reversible introduction of electrostatic charges in the βCD dextran by complex formation with 1-adamantanecarboxylic acid prior to surface grafting resulted in a thinner and denser film, presumably by decompaction of the polymers. Total internal reflection fluorescence spectroscopy (TIRF) was employed to evaluate the accessibility of βCD cavities to the fluorescent probe 2-anilinonaphthalene-6-sulfonic acid. Only a minor fraction of the βCD cavities was accessible in the thicker and less dense films; however, accessibility was largely improved with increased ionic strength using NaCl up to 1 M. Antifouling properties of the βCD dextran polymer films were assessed by TIRF real-time monitoring, using bovine serum albumin as a model protein, and showed a 5- to 10-fold reduction in nonspecific adsorption as compared to a bare quartz surface with the degree of reduction reflecting film thickness and interfacial polymer density.
authors: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University , Frederik Bajers Vej 7H, DK-9220 Aalborg East, Denmark.
authors: Lars W
authors: LW
authors: Städe
authors: Thorbjørn T
authors: TT
authors: Nielsen
authors: Laurent
authors: L
authors: Duroux
authors: Mogens
authors: M
authors: Hinge
authors: Kyoko
authors: K
authors: Shimizu
authors: Leonid
authors: L
authors: Gurevich
authors: Peter K
authors: PK
authors: Kristensen
authors: Christer
authors: C
authors: Wingren
authors: Kim L
authors: KL
authors: Larsen
mesh_terms: D000327:Adsorption; D000818:Animals; D057219:Biofouling; D002417:Cattle; D003911:Dextrans / Q000737:chemistry*; D009994:Osmolar Concentration; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D003911:Dextrans; D011108:Polymers; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; C031215:betadex
keywords: dextran; nonspecific adsorption; polymer films; protein immobilization; total internal reflection fluorescence spectroscopy; β-cyclodextrin