title: Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury.
abstract: New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/ reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Qi
authors: Q
authors: Gao
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Yonghui
authors: Y
authors: Liang
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Xiaoyu
authors: X
authors: Yang
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Guifeng
authors: G
authors: Liu
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Xiaoxue
authors: X
authors: Li
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Benqing
authors: B
authors: Zhu
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Jian
authors: J
authors: Liu
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Maoguang
authors: M
authors: Yang
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Weiwei
authors: W
authors: Xia
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Jian
authors: J
authors: Dong
authors: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors: Jianhang
authors: J
authors: Jiao
keywords: ischemia/reperfusion; mass spectrometry; neural regeneration; proteomics; rabbit; spinal cord injury; two-dimensional gel electrophoresis
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