title: Enamel and dentin mineralization in familial hypophosphatemic rickets: a micro-CT study.
abstract: OBJECTIVE
  The aim of the present study was to analyse the mineralization pattern of enamel and dentin in patients affected by X-linked hypophosphatemic rickets (XLHR) using micro-CT (µCT), and to associate enamel and dentin mineralization in primary and permanent teeth with tooth position, gender and the presence/absence of this disease.
  
  
  METHODS
  19 teeth were collected from 5 individuals from the same family, 1 non-affected by XLHR and 4 affected by XLHR. Gender, age, tooth position (anterior/posterior) and tooth type (deciduous/permanent) were recorded for each patient. Following collection, teeth were placed in 0.1% thymol solution until µCT scan. Projection images were reconstructed and analysed. A plot profile describing the greyscale distance relationship in µCT images was achieved through a line bisecting each tooth in a region with the presence of enamel and dentin. The enamel and dentin mineralization densities were measured and compared. Univariate ANOVA and post hoc Tukey tests were used for all comparisons.
  
  
  RESULTS
  Teeth of all affected patients presented dentin with a different mineralization pattern compared with the teeth of healthy patients with dentin defects observed next to the pulp chambers. Highly significant differences were found for gray values between anterior and posterior teeth (p < 0.05), affected and non-affected (p < 0.05), as well as when position and disease status were considered (p < 0.05).
  
  
  CONCLUSIONS
  In conclusion, the mineralization patterns of dentin differed when comparing teeth from patients with and without FHR, mainly next to pulp chambers where areas with porosity and consequently lower mineral density and dentin defects were found.
authors: 1 Clinical Dentistry Department, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: T R
authors: TR
authors: Ribeiro
authors: F W G
authors: FW
authors: Costa
authors: E C S
authors: EC
authors: Soares
authors: J R
authors: JR
authors: Williams
authors: C S R
authors: CS
authors: Fonteles
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003743:Dental Enamel / Q000000981:diagnostic imaging / Q000473:pathology; D003804:Dentin / Q000000981:diagnostic imaging / Q000473:pathology; D053098:Familial Hypophosphatemic Rickets / Q000000981:diagnostic imaging* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D014074:Tooth Calcification / Q000502:physiology*; D055114:X-Ray Microtomography
keywords: X-ray microtomography; familial hypophosphatemic rickets; radiology; tooth; tooth calcification
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