title: Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells.
abstract: Carbonated apatite (CO3Ap) is the inorganic component of bone. We have proposed a new method for the fabrication of CO3Ap blocks based on a dissolution-precipitation method using a synthetic precursor. The aim of this study is to examine the effects of low crystalline CO3Ap on initial cell attachment, proliferation and osteoblastic differentiation of human bone marrow cells (hBMCs) using sintered hydroxyapatite and tissue culture plates as controls. Initial cell attachment and proliferation were assessed with a MTT assay. Expression of osteoblastic markers was examined by reverse transcription-polymerase chain reaction. XRD and FT-IR results showed formation of B-type carbonate apatite with lower crystallinity. No difference was observed for initial cell attachment between HAp and CO3Ap discs. hBMSC attached more significantly on tissue culture plate than on HAp and CO3Ap discs. The number of cells on HAp was higher than that on CO3Ap until day 7, after which the number of cells was similar. hBMSC proliferated more significantly on tissue culture plate than on HAp and CO3Ap discs. In contrast, hBMCs incubated on CO3Ap demonstrated much higher expression of osteoblastic markers of differentiation, such as type I collagen, alkaline phosphatase, osteopontin and osteocalcin, than hBMCs on HAp. On the tissue culture plate, they were not any change throughout the culture period. These results demonstrated that low crystalline CO3Ap exhibit higher osteoinductivity than HAp.
authors: Subdivision of Molecular Oral Medicine, Division of Integrated Sciences of Translational Research, Department of Oral Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8504, Japan, hnagai@tokushima-u.ac.jp.
authors: Hirokazu
authors: H
authors: Nagai
authors: Masako
authors: M
authors: Kobayashi-Fujioka
authors: Kenji
authors: K
authors: Fujisawa
authors: Go
authors: G
authors: Ohe
authors: Natsumi
authors: N
authors: Takamaru
authors: Kanae
authors: K
authors: Hara
authors: Daisuke
authors: D
authors: Uchida
authors: Tetsuya
authors: T
authors: Tamatani
authors: Kunio
authors: K
authors: Ishikawa
authors: Youji
authors: Y
authors: Miyamoto
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001031:Apatites / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D015415:Biomarkers; D018786:Bone Substitutes; D024042:Collagen Type I; D015675:Osteocalcin; D053495:Osteopontin; C030782:carboapatite; D017886:Durapatite; D000469:Alkaline Phosphatase
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