title: Experimental study on the mechanical interaction between silicon neural microprobes and rat dura mater during insertion.
abstract: In vivo insertion experiments are essential to optimize novel neural implants. Our work focuses on the interaction between intact dura mater of rats and as-fabricated single-shaft silicon microprobes realized by deep reactive ion etching. Implantation parameters like penetration force and dimpling through intact dura mater were studied as a function of insertion speed, microprobe cross-section, tip angle and animal age. To reduce tissue resistance, we proposed a unique tip sharpening technique, which was also evaluated in in vivo insertion tests. By doubling the insertion speed (between 1.2 and 10.5 mm/min), an increase of 10-35% in penetration forces was measured. When decreasing the cross-section of the microprobes, penetration forces and dimpling was reduced by as much as 30-50% at constant insertion speeds. Force was noticed to gradually decrease by decreasing tip angles. Measured penetration forces through dura mater were reduced even down to 11±3 mN compared to unsharpened (49±13 mN) probes by utilizing our unique tip sharpening technique, which is very close to exerted penetration force in the case of retracted dura (5±1.5 mN). Our findings imply that age remarkably alters the elasticity of intact dura mater. The decreasing stiffness of dura mater results in a significant rise in penetration force and decrease in dimpling. Our work is the first in vivo comparative study on microelectrode penetration through intact and retracted dura mater.
authors: MEMS Lab, Institute for Technical Physics & Material Science, RCNS, HAS, P.O.Box 49, Budapest, 1525, Hungary, fekete@mfa.kfki.hu.
authors: Z
authors: Z
authors: Fekete
authors: A
authors: A
authors: Németh
authors: G
authors: G
authors: Márton
authors: I
authors: I
authors: Ulbert
authors: A
authors: A
authors: Pongrácz
mesh_terms: D000818:Animals; D004388:Dura Mater / Q000502:physiology* / Q000601:surgery; D055119:Elastic Modulus / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D011312:Pressure; D019919:Prosthesis Implantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013129:Spinal Puncture / Q000295:instrumentation* / Q000379:methods*; D013314:Stress, Mechanical
references: J Neural Eng. 2006 Sep;3(3):196-207
references: 16921203
references: J Neurosci Methods. 2012 Oct 15;211(1):22-30
references: 22971352
references: J Neural Eng. 2012 Feb;9(1):016005
references: 22156141
references: J Neurotrauma. 2003 Nov;20(11):1163-77
references: 14651804
references: J Neurosci Methods. 2005 Mar 30;142(2):169-76
references: 15698656
references: Biosens Bioelectron. 2007 Mar 15;22(8):1783-90
references: 17027251
references: IEEE Trans Biomed Eng. 1990 May;37(5):474-81
references: 2345003
references: J Neurosci Methods. 2007 Mar 15;160(2):276-87
references: 17084461
references: J Neurophysiol. 2005 Jan;93(1):570-9
references: 15229215
references: Med Eng Phys. 2007 May;29(4):413-31
references: 16938481
references: IEEE Trans Biomed Eng. 1992 Jun;39(6):635-43
references: 1601445
references: J Neurosci Methods. 2001 Dec 15;112(2):83-94
references: 11716944
references: Clin Phys Physiol Meas. 1981 Aug;2(3):197-203
references: 7338023
references: IEEE Trans Biomed Eng. 2006 May;53(5):934-40
references: 16686416
references: Ann Biomed Eng. 1992;20(4):413-22
references: 1510293
references: Conf Proc IEEE Eng Med Biol Soc. 2007;2007:4711-4
references: 18003057
references: IEEE Trans Biomed Eng. 2011 Nov;58(11):3250-9
references: 21896383
references: IEEE Trans Biomed Eng. 2009 Jan;56(1):45-53
references: 19224718
references: J Biomech. 2004 Sep;37(9):1339-52
references: 15275841
references: Proc IEEE RAS EMBS Int Conf Biomed Robot Biomechatron. 2008 Oct 19;:224-231
references: 22020139
references: J Biomech. 2004 Aug;37(8):1155-63
references: 15212920
references: Brain Res. 2003 Sep 5;983(1-2):23-35
references: 12914963
references: IEEE Trans Biomed Eng. 2000 Mar;47(3):281-9
references: 10743769
references: IEEE Trans Biomed Eng. 2008 Sep;55(9):2258-67
references: 18713695
references: Sensors (Basel). 2008 Oct 25;8(10 ):6704-6726
references: 27873894
references: J R Soc Interface. 2005 Jun 22;2(3):159-67
references: 16849176
references: Biosens Bioelectron. 2009 Apr 15;24(8):2410-6
references: 19167206