title: Effects of manganese deficiency on the microstructure of proximal tibia and OPG/RANKL gene expression in chicks.
abstract: Manganese (Mn) deficiency can result in perosis in chicks, but the mechanism of Mn deficiency on tibia development remains poorly understood. Ninety one-day-old Arbor Acres male broiler chickens administered with control diet (60 mg Mn/kg) and Mn-deficient diets (40 mg Mn/kg, 8.7 mg Mn/kg) to investigate the effects of Mn deficiency on morphology of tibia and related signal transduction pathways in broiler chickens. At the age of 42 days, the bone trabecula, damaged osteoblasts and OPG/RANKL mRNA expression levels were investigated by histological assessment, electron microscopic examination and real-time quantitative PCR analysis, respectively. Results of histological observations showed that decreased trabecular thickness, trabecular number and trabecular bone area (%) together with increased trabecular bone separation were involved in perosis induced by Mn deficiency. The most striking ultrastructural modifications involved disruption of nuclear membrane and mitochondria outer membrane, loss of mitochondrion cristae and alteration in endoplasmic reticulum in osteoblasts of the Mn-deficient groups. Likewise, Mn deficiency results in a significant (P < 0.05) decrease in the relative mRNA expression levels of OPG and RANKL with a significantly higher RANKL/OPG ratio (P < 0.05). In conclusion, Mn deficiency can affect the development of tibia in broiler chickens, leading to metaphyseal osteoporosis which may be due to decreased OPG/RANKL mRNA expression.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Taian, 271018, People's Republic of China.
authors: Ran
authors: R
authors: Liu
authors: Cong
authors: C
authors: Jin
authors: Zhenyong
authors: Z
authors: Wang
authors: Zhaojun
authors: Z
authors: Wang
authors: Jian
authors: J
authors: Wang
authors: Lin
authors: L
authors: Wang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D004032:Diet / Q000662:veterinary*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D008345:Manganese / Q000172:deficiency*; D010006:Osteoblasts / Q000473:pathology / Q000648:ultrastructure; D053244:Osteoprotegerin / Q000235:genetics*; D053245:RANK Ligand / Q000235:genetics*; D015398:Signal Transduction; D013977:Tibia / Q000473:pathology / Q000648:ultrastructure*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D008345:Manganese
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