title: The effect of magnesium sulfate on the placental corticotropin-releasing factor (CRF) and CRF binding protein mRNA expression in perfused human placental cotyledon.
abstract: OBJECTIVE
  Stress stimuli and inflammation influence the secretion of the placental corticotropin-releasing factor CRF (CRF) that has a significant role in controlling the timing of birth. The CRF-binding protein (CRF-BP) binds CRF with high affinity and inhibits its activity. Magnesium sulfate (MgSO4) has been known to ameliorate maternal, fetal and gestational tissue-associated inflammatory response. We aimed to study the effect of MgSO4 on the CRF and CRF-BP mRNA expression levels in perfused human cotyledon.
  
  
  METHODS
  Placentas from elective caesarean section were obtained and selected cotyledons were cannulated and dually perfused ex-vivo within 30 min. MgSO4 (7 mg/dl) was added to the maternal reservoir. Each perfusion experiment was conducted for 180 min. At the end of the experiment, RNA was extracted from the perfused cotyledon, and RT-PCR was performed to quantify the expression of CRF and CRF-BP. Human HPRT gene served as a reference gene.
  
  
  RESULTS
  Perfusion with MgSO4 (n = 3) induced a significantly lower CRF and higher CRF-BP mRNA expression compared to placentas perfused only with medium (n = 3).
  
  
  CONCLUSIONS
  In the human placenta, MgSO4 possibly exerts its action through different modulation on the CRF and CRF-BP expression.
authors: a Department of Obstetrics & Gynecology , The Edith Wolfson Medical Center, Holon Affiliated with Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv , Israel and.
authors: Nesia
authors: N
authors: Kropach
authors: b Department of Neurobiology , Weizmann Institute of Science , Rehovot , Israel.
authors: Dorin
authors: D
authors: Holtzman
authors: a Department of Obstetrics & Gynecology , The Edith Wolfson Medical Center, Holon Affiliated with Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv , Israel and.
authors: Dana
authors: D
authors: Vitner
authors: a Department of Obstetrics & Gynecology , The Edith Wolfson Medical Center, Holon Affiliated with Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv , Israel and.
authors: Jacob
authors: J
authors: Bar
authors: b Department of Neurobiology , Weizmann Institute of Science , Rehovot , Israel.
authors: Alon
authors: A
authors: Chen
authors: a Department of Obstetrics & Gynecology , The Edith Wolfson Medical Center, Holon Affiliated with Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv , Israel and.
authors: Michal
authors: M
authors: Kovo
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008278:Magnesium Sulfate / Q000494:pharmacology*; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; C067133:corticotropin releasing factor-binding protein; D008278:Magnesium Sulfate; D003346:Corticotropin-Releasing Hormone
keywords: Corticotropin-releasing factor; magnesium sulfate; placenta