title: Genetically engineered mouse models to study prostate cancer.
abstract: Genetically engineered mouse models have become fundamental tools in the basic and translational research of prostate cancer. There is a plethora of models available to dissect the genetic alterations and aberrant signaling events associated with human prostate cancer and, furthermore, to investigate new and "personalized" therapies to treat the disease. In this chapter, we discuss some of the models recently and currently used to study prostate cancer in vivo, and some considerations when selecting an appropriate model to investigate particular aspects of the disease. We describe the methods required to isolate prostate tumors and conduct basic characterization of the tumor to determine tumor load and histopathology. We also discuss important aspects to be considered when processing samples for further analysis.
authors: The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, G61 1BD, Bearsden, Glasgow, UK.
authors: Elspeth A
authors: EA
authors: Brzezinska
authors: Colin
authors: C
authors: Nixon
authors: Rachana
authors: R
authors: Patel
authors: Hing Y
authors: HY
authors: Leung
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004210:Dissection; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D005557:Formaldehyde; D005818:Genetic Engineering / Q000379:methods*; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling; D016610:Tissue Embedding; D016707:Tissue Fixation
chemical_list: D019394:Ki-67 Antigen; D005557:Formaldehyde; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin