title: Preliminary report of HLA (DNA) typing of Nigerians using sequence-specific primer technique.
abstract: OBJECTIVE
  This communication is an attempt to present the experience and a preliminary report of results over a one-year period.
  
  
  METHODS
  From December 2011 to December 2012, a prospective determination of the HLA types of 20 individuals referred to the Tissue Typing Laboratory of the Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC), Ile-Ife was done. These consisted of prospective transplant recipients, their donors, and a migrant pair for kinship determination. DNA was extracted from the client's peripheral blood sample, using the QIAmp Blood DNA Mini kit, (Qiagen). PCR was done using OlerupR low-resolution PCR-SSP typing kit. The PCR product was resolved in 2% agarose gel, and the bands visualised under UV light. The HLA types were determined using provided tables and/or Helmberg software. Data were presented using descriptive statistics whileHLA antigen frequency (AF) was expressed in percentage and gene frequency (GF) was determined using square root method (1-(1-AF)1/2).
  
  
  RESULTS
  A total of 20 individuals (13males and 7females) consisting of seven renal transplant recipients and seven prospective donors; a stem cell recipient and three donors and a migrant pair for kinship determination were typed. Age ranged from 4-65 years. 44 HLA alleles were detected, while HLA-A, B, C, DRB1 and DQB1 were 7, 10, 11, 8, 8 alleles respectively. The alleles were heterogeneous in distribution while 6 antigens (HLA-A*02, B*30, C*15, DRB1*03, DRB1*08 and DQB1*06) were having frequencies e"25%.
  
  
  CONCLUSIONS
  This report confirms that DNA-based HLA typing is feasible locally, andit was observed that renal transplantation procedure is the most frequent indication. The HLA antigens observed to have very high frequencies (e"25% frequency) in this population were HLA-A*02, B*30, C*15, DRB1*03, DRB1*08 and DQB1*06. There is a strong need to develop a broad-based HLA data bank for Nigeria to further strengthening her transplantation programmes.
authors: Department of Haematology and Immunology, Obafemi Awolowo University and Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria.
authors: R A
authors: RA
authors: Bolarinwa
authors: A A
authors: AA
authors: Oyekunle
authors: F A
authors: FA
authors: Arogundade
authors: A A
authors: AA
authors: Sanusi
authors: L
authors: L
authors: Salawu
authors: R A
authors: RA
authors: Togun
authors: N O
authors: NO
authors: Akinola
authors: M A
authors: MA
authors: Durosinmi
authors: A A
authors: AA
authors: Akinsola
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting / Q000379:methods*; D015343:DNA Probes, HLA / Q000032:analysis*; D005260:Female; D005787:Gene Frequency; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009549:Nigeria; D016377:Organ Transplantation; D011446:Prospective Studies; D014019:Tissue Donors / Q000706:statistics & numerical data*; D066027:Transplant Recipients / Q000706:statistics & numerical data*; D055815:Young Adult
chemical_list: D015343:DNA Probes, HLA