title: Oxidative stress-associated senescence in dermal papilla cells of men with androgenetic alopecia.
abstract: Dermal papilla cells (DPCs) taken from male androgenetic alopecia (AGA) patients undergo premature senescence in vitro in association with the expression of p16(INK4a), suggesting that DPCs from balding scalp are more sensitive to environmental stress than nonbalding cells. As one of the major triggers of senescence in vitro stems from the cell "culture shock" owing to oxidative stress, we have further investigated the effects of oxidative stress on balding and occipital scalp DPCs. Patient-matched DPCs from balding and occipital scalp were cultured at atmospheric (21%) or physiologically normal (2%) O2. At 21% O2, DPCs showed flattened morphology and a significant reduction in mobility, population doubling, increased levels of reactive oxygen species and senescence-associated β-Gal activity, and increased expression of p16(INK4a) and pRB. Balding DPCs secreted higher levels of the negative hair growth regulators transforming growth factor beta 1 and 2 in response to H2O2 but not cell culture-associated oxidative stress. Balding DPCs had higher levels of catalase and total glutathione but appear to be less able to handle oxidative stress compared with occipital DPCs. These in vitro findings suggest that there may be a role for oxidative stress in the pathogenesis of AGA both in relation to cell senescence and migration but also secretion of known hair follicle inhibitory factors.
authors: Centre for Cutaneous Research, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors: James H
authors: JH
authors: Upton
authors: Centre for Cutaneous Research, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors: Rosalind F
authors: RF
authors: Hannen
authors: Centre for Cutaneous Research, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors: Adiam W
authors: AW
authors: Bahta
authors: Farjo Medical Centre, Manchester, UK.
authors: Nilofer
authors: N
authors: Farjo
authors: Farjo Medical Centre, Manchester, UK.
authors: Bessam
authors: B
authors: Farjo
authors: Centre for Cutaneous Research, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK. Electronic address: m.p.philpott@qmul.ac.uk.
authors: Michael P
authors: MP
authors: Philpott
mesh_terms: D000328:Adult; D000505:Alopecia / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D020405:Dermis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012535:Scalp / Q000473:pathology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D017382:Reactive Oxygen Species; D002374:Catalase; D005978:Glutathione; D010100:Oxygen
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