title: Cellulase-assisted extraction, characterization, and bioactivity of polysaccharides from Polygonatum odoratum.
abstract: Cellulase-assisted extraction of polysaccharides from Polygonatum odoratum (CPP) was optimized by response surface methodology (RSM) and the extracted CPP's preliminary chemical characteristics, as well as antioxidant and immunomodulatory activities were also investigated. The optimal extraction parameters comprised an extraction temperature of 58.21 °C, an extraction time of 3.18 h, pH value of 5.8, and cellulase amount of 6.0%. Under these conditions, the relative yield was 15.76%, higher than the yield achieved with hot water extracted polysaccharide (HPP). Chemical composition analysis demonstrated that CPP and HPP consisted of mannose, glucosamine, rhamnose, glucose, galactose, and arabinose with a molecular ratio of 7.80:1.08:1.63:65.93:3.58:1.00 and 11.22:0.23:0.23:17.59:2.73:9.10, respectively. The molecular weight distribution of CPP was lower and more homogeneous compared with HPP. CPP exhibited stronger antioxidant activities than HPP, including DPPH radical scavenging activity and reducing power. Both CPP and HPP could significantly promote the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells in vitro. These results indicate that the cellulase-assisted extraction method influenced the physicochemical characteristics, and consequently, the functional activities of polysaccharides, suggesting the cellulose-assisted method may be a viable option for extraction polysaccharides from P. odoratum.
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Xin
authors: X
authors: Liu
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Miansong
authors: M
authors: Zhang
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Kai
authors: K
authors: Guo
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Airong
authors: A
authors: Jia
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Yaping
authors: Y
authors: Shi
authors: Fengxian Hospital Affiliated to Southern Medical University, 6600 NanFeng Road, Shanghai 201499, China. Electronic address: qqsohu@163.com.
authors: Ganglong
authors: G
authors: Gao
authors: Fengxian Hospital Affiliated to Southern Medical University, 6600 NanFeng Road, Shanghai 201499, China. Electronic address: hope1126@hotmail.com.
authors: Zhenliang
authors: Z
authors: Sun
authors: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China.
authors: Changheng
authors: C
authors: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007155:Immunologic Factors / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D031384:Polygonatum / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D013696:Temperature; D013997:Time Factors
chemical_list: D000975:Antioxidants; D007155:Immunologic Factors; D011134:Polysaccharides; D002480:Cellulase
keywords: Cellulase-assisted extraction; Polygonatum odoratum; Polysaccharide