title: Serum sulfatide abnormality is associated with increased oxidative stress in hemodialysis patients.
abstract: Sulfatides are major glycosphingolipids of lipoproteins that influence atherosclerosis and blood coagulation. Our previous cross-sectional study of hemodialysis patients showed that serum sulfatide levels decreased markedly with increasing duration of hemodialysis treatment, which may contribute to the development of cardiovascular disease. However, this past study could not demonstrate the time-dependent change in serum sulfatide levels in each patient, and the underlying mechanism is unknown. To confirm the time-dependent aggravation of serum sulfatide abnormality, 95 stable hemodialysis outpatients were followed up for 3 years. To show the underlying mechanisms, we statistically analyzed correlations between serum sulfatide levels and clinical factors, including an oxidative stress marker, malondialdehyde. Serum sulfatides were quantified by mass spectrometry after conversion to lysosulfatides. Malondialdehyde was measured using a colorimetric assay. The results showed a time-dependent decrease in serum sulfatide levels associated with increased malondialdehyde levels, although the absolute level of serum malondialdehyde does not determine the baseline level of serum sulfatides. Multiple linear regression analysis showed a significant correlation only between the time-dependent change in serum sulfatide levels and the time-dependent change in serum malondialdehyde levels. This study demonstrated, for the first time, a time-dependent aggravation of serum sulfatide abnormality in hemodialysis patients, as well as the potential relationship between serum sulfatide abnormality and increasing oxidative stress. These findings suggest that oxidative stress might be an aggravating factor in serum sulfatide abnormality. As continuation of hemodialysis treatment hardly improves abnormal serum sulfatide levels or increased oxidative stress, development of novel therapeutic strategies may be important.
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.|Department of Human Anatomy, Hebei Medical University, Shijiazhuang, Hebei, China.
authors: Huang
authors: H
authors: Yuzhe
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.|Department of Nephrology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors: Yuji
authors: Y
authors: Kamijo
authors: Department of Nephrology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors: Koji
authors: K
authors: Hashimoto
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.|Department of Nephrology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors: Makoto
authors: M
authors: Harada
authors: Department of Nephrology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors: Taro
authors: T
authors: Kanno
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.|Department of Nutritional Science, Nagano Prefectural College, Nagano, Nagano, Japan.
authors: Eiko
authors: E
authors: Sugiyama
authors: Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, Ina, Saitama, Japan.
authors: Mamoru
authors: M
authors: Kyogashima
authors: Kidney Disease and Dialysis Center, Aizawa Hospital, Matsumoto, Nagano, Japan.
authors: Tomomasa
authors: T
authors: Oguchi
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.
authors: Takero
authors: T
authors: Nakajima
authors: Department of Nephrology, Kanno Dialysis and Vascular Access Clinic, Matsumoto, Nagano, Japan.
authors: Yutaka
authors: Y
authors: Kanno
authors: Department of Metabolic Regulation, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.
authors: Toshifumi
authors: T
authors: Aoyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D002318:Cardiovascular Diseases / Q000097:blood*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000009:adverse effects* / Q000379:methods; D013433:Sulfoglycosphingolipids / Q000097:blood*
chemical_list: D015415:Biomarkers; D013433:Sulfoglycosphingolipids
keywords: Cardiovascular disease; hemodialysis; malondialdehyde; oxidative stress; serum sulfatide level