title: Evaluation of 'cattle' and 'Indian Bison' type antigens of Mycobacterium avium subspecies paratuberculosis for diagnosis of bovine Johne's disease using 'indigenous ELISA' and AGPT.
abstract: Two antigens ('cattle' type and 'Indian Bison' type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne's disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.
authors: Donna
authors: D
authors: Pahangchopi
authors: Ran Vir
authors: RV
authors: Singh
authors: Shoor Vir
authors: SV
authors: Singh
authors: Paritosh
authors: P
authors: Das
authors: Deepak
authors: D
authors: Sharma
authors: Tarun
authors: T
authors: Sardana
authors: Naveen
authors: N
authors: Kumar
authors: Kundan Kumar
authors: KK
authors: Chaubey
authors: Saurabh
authors: S
authors: Gupta
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D016164:Bison; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005838:Genotype; D054884:Host-Pathogen Interactions / Q000276:immunology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D010283:Paratuberculosis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D011233:Precipitin Tests / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial