title: Green tea changes serum and liver metabolomic profiles in mice with high-fat diet-induced obesity.
abstract: METHODS
  Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT-induced changes in serum and liver metabolomes associated with the anti-obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT-fed obese mice to elucidate the relationship between GT consumption and obesity prevention.
  
  
  RESULTS
  Serum and liver metabolites were analyzed in mice fed normal diet, high-fat diet (HFD), HFD with GT, and HFD with crude catechins, using LC-quadrupole TOF MS. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD-fed mice. We proposed an HFD-induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β-oxidation: carnitine palmitoyltransferase-1 and -2, acyl-coenzyme A dehydrogenase, and acetyl-coenzyme A acyltransferase. The results showed that HFD-induced abnormal mitochondrial β-oxidation was moderated by the consumption of caffeine- and theanine-enriched GT.
  
  
  CONCLUSIONS
  Results of LC/MS-based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti-obesity effects of GT.
authors: Korea Food Research Institute, Bundang-gu, Seongnam, Gyeonggi, Republic of Korea.
authors: Lan-Sook
authors: LS
authors: Lee
authors: Ji Hea
authors: JH
authors: Choi
authors: Mi Jeong
authors: MJ
authors: Sung
authors: Jin-Young
authors: JY
authors: Hur
authors: Haeng Jeon
authors: HJ
authors: Hur
authors: Jong-Dae
authors: JD
authors: Park
authors: Young-Chan
authors: YC
authors: Kim
authors: Eun-Ji
authors: EJ
authors: Gu
authors: Byungjin
authors: B
authors: Min
authors: Hyun-Jin
authors: HJ
authors: Kim
mesh_terms: D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism; D042964:Acyl-CoA Dehydrogenase / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D055442:Metabolome; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015999:Multivariate Analysis; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology; D013662:Tea / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D020738:Leptin; D013662:Tea; D014280:Triglycerides; D002784:Cholesterol; D042964:Acyl-CoA Dehydrogenase; D000102:Acetyl-CoA C-Acyltransferase; D002334:Carnitine O-Palmitoyltransferase
keywords: Acetyl-CoA acyltransferase 2; Fatty acid β-oxidation; Green tea; Metabolomics; Obesity