title: Effects of Temperature and Hypochlorite Concentration on the Rate of Collagen Dissolution.
abstract: BACKGROUND
  The aim of this research was to quantify the effects of temperature on the rate of collagen dissolution in different concentrations of sodium hypochlorite solution.
  
  
  METHODS
  A customized laboratory apparatus was used to immerse 23 samples of reticulated collagen matrices and 20 samples of non-reticulated collagen matrices in a continuously agitated solution of 1%-5% (m/v) sodium hypochlorite at 20°C-35°C. The time needed for complete dissolution of each sample was used to generate mathematical equations that described the effects of temperature and hypochlorite concentration on the dissolution of collagen.
  
  
  RESULTS
  Both temperature and concentration of sodium hypochlorite were positively correlated with the rate of collagen dissolution. The same speed of dissolution was obtained for 5% sodium hypochlorite at 20°C, 4% solution at 20.8°C, 3% solution at 23.5°C, 2% solution at 26.9°C, and 1% solution at 36°C.
  
  
  CONCLUSIONS
  Treatment protocols that involve sodium hypochlorite, which is the preferred root canal irrigant, should consider the important effects caused by variations in the use of different irrigant temperatures and concentrations. Further investigation is required to establish the optimal concentration and temperature of sodium hypochlorite solution for root canal irrigation.
authors: Park Street Dental Practice, Weymouth, Dorset, United Kingdom. Electronic address: info@dentist-weymouth.co.uk.
authors: Diana
authors: D
authors: Dumitriu
authors: Mass Transfer Department, Politechnica University, Bucharest, Romania.
authors: Tanase
authors: T
authors: Dobre
mesh_terms: D003094:Collagen / Q000187:drug effects*; D006801:Humans; D006997:Hypochlorous Acid / Q000008:administration & dosage* / Q000737:chemistry*; D012388:Root Canal Irrigants / Q000008:administration & dosage* / Q000737:chemistry*; D012996:Solutions; D013696:Temperature
chemical_list: D012388:Root Canal Irrigants; D012996:Solutions; D006997:Hypochlorous Acid; D003094:Collagen
keywords: Collagen; root canal irrigant; sodium hypochlorite; speed of dissolution; tissue dissolution