title: Investigating the role of human serum albumin protein pocket on the excited state dynamics of indocyanine green using shaped femtosecond laser pulses.
abstract: Differences in the excited state dynamics of molecules and photo-activated drugs either in solution or confined inside protein pockets or large biological macromolecules occur within the first few hundred femtoseconds. Shaped femtosecond laser pulses are used to probe the behavior of indocyanine green (ICG), the only Food and Drug Administration (FDA) approved near-infrared dye and photodynamic therapy agent, while free in solution and while confined inside the pocket of the human serum albumin (HSA) protein. Experimental findings indicate that the HSA pocket hinders torsional motion and thus mitigates the triplet state formation in ICG. Low frequency vibrational motion of ICG is observed more clearly when it is bound to the HSA protein.
authors: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA. dantus@msu.edu.
authors: Muath
authors: M
authors: Nairat
authors: Arkaprabha
authors: A
authors: Konar
authors: Marie
authors: M
authors: Kaniecki
authors: Vadim V
authors: VV
authors: Lozovoy
authors: Marcos
authors: M
authors: Dantus
mesh_terms: D001665:Binding Sites; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D007834:Lasers; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012709:Serum Albumin; D007208:Indocyanine Green