title: Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes.
abstract: Artificial insemination (AI) of sows with frozen-thawed semen usually results in lower pregnancy rates and litter sizes than the use of liquid preserved semen. The present study evaluated the effectiveness of vulvar skin temperature changes as a predictor of ovulation in sows and determined the fertility rates obtained after AI with frozen-thawed semen supplemented with rosmarinic acid (RA). Semen was collected from mature boars and cryopreserved in experimental extenders supplemented with or without 105 μM of RA. Multiparous sows were inseminated with a single dose of semen when vulvar skin temperature decreased to a value below 35 °C. Intrauterine insemination was performed using 1.5 × 109 spermatozoa. The sows were slaughtered 48 h after AI and the embryos and oocytes were recovered from the oviducts. Total and progressive motility, viability and acrosome integrity were significantly (P < 0.05) higher in RA-supplemented semen samples compared with the control. Fertilisation occurred in all sows inseminated in the study, although there were no significant differences between the experimental groups. Sows inseminated with RA-supplemented semen showed a slight increase in the number of embryos recovered as compared to sows inseminated with control semen. In conclusion, insemination according to vulvar skin temperature changes resulted in successful fertilisation in all sows, although supplementation of the freezing media with RA did not improve the fertilising ability of frozen-thawed boar sperm.
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Victoria
authors: V
authors: Luño
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Lydia
authors: L
authors: Gil
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Maite
authors: M
authors: Olaciregui
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Juan
authors: J
authors: Grandía
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Trinidad
authors: T
authors: Ansó
authors: Universidad de Zaragoza Department of Animal Pathology C/Miguel Servet 177 50013 Zaragoza Spain.
authors: Ignacio
authors: I
authors: De Blas
keywords: Artificial insemination; fertility; frozen-thawed boar sperm; rosmarinic acid; temperature