title: Quantification of glycyrrhizin in anti-stress herbal formulations by validate HPTLC method: a rational paradigm towards quality control of herbals.
abstract: In the present study an analytical method of high-performance thin-layer chromatography (HPTLC) has been developed for quantification of glycyrrhizin for marketed antistressliquorice root capsules (LRC) and herbal tea (HT). Chromatography was performed by using mobile phase ethyl acetate (EA): glacial acetic acid (GAA): Methanol (MeOH): water (H(2)O) in proportion of 6:2:2:1, v/v/v/v. The developed plate was scanned and quantified densitometrically at absorption maxima 254nm. The method was validated for various analytical parameters viz. precision, accuracy, recovery, robustness, specificity, detection and quantification limits. The developed system was found to give compact spot for glycyrrhizin (R(f)= 0.33± 0.001). The linearity relationship was described by the equation Y=6.841X+ 70.428. The limit of detection (34 ng band(-1)), limit of quantification (101 ng band(-1)), recovery (99.4-99.8%), and precision (<1.84% and <1.62%; intraday and interday, respectively) were found satisfactory for glycyrrhizin. Linearity range for glycyrrhizin was 100-600ng (r(2)=0.998). The amount of glycyrrhizin was estimated by comparing the peak area of standard and the same was present in crude extract. The content of glycyrrhizin was estimated as 11.4% and 4.7% w/w in sample LRC and HT, respectively. The proposed method will be useful to quantify the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug.
authors: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, Saudi Arabia.
authors: Nasir Ali
authors: NA
authors: Siddiqui
authors: Perwez
authors: P
authors: Alam
authors: Mohammad Khalid
authors: MK
authors: Parvez
authors: Omer Ahmed
authors: OA
authors: Basudan
authors: Mohammed Salem
authors: MS
authors: Al-Dosari
authors: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, Saudi Arabia.
authors: Adnan Jathlan
authors: AJ
authors: Al-Rehaily
authors: Mohammed Fahad
authors: MF
authors: Al-Ajmi
authors: Center of Excellence in Biotechnology Research, King Saud University, P.O. Box 2460, Riyadh, Saudi Arabia.
authors: Faiyaz
authors: F
authors: Shakeel
mesh_terms: D001628:Beverages / Q000032:analysis*; D002214:Capsules; D002855:Chromatography, Thin Layer / Q000379:methods*; D019695:Glycyrrhizic Acid / Q000008:administration & dosage / Q000032:analysis*; D011786:Quality Control; D013315:Stress, Psychological / Q000188:drug therapy*
chemical_list: D002214:Capsules; D019695:Glycyrrhizic Acid