title: miRNA-181 regulates embryo implantation in mice through targeting leukemia inhibitory factor.
abstract: Embryo implantation is a crucial step in mammalian reproduction. However, little is known regarding the physiological roles of microRNAs in the regulation of embryo implantation. Here we show that a minimum uterine expression of miR-181 is essential for the onset of embryo implantation. Both transient and prolonged transgenic expression of miR-181 led to impaired implantation, which can be rescued by exogenous administration of leukemia inhibitory factor (LIF). Mechanistically, miR-181 is able to directly target LIF and downregulate LIF expression, thereby inhibiting embryo implantation. We also show that miR-181 expression is regulated by the transcriptional factor Emx2, and the Emx2-miR-181 axis plays an important role in regulating embryo implantation. Taken together, these results reveal a novel function of miR-181 in embryo implantation through the regulation of LIF, and also indicate a potential link between miR-181 dysregulation and human embryo implantation defects.
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China.
authors: Bo
authors: B
authors: Chu
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China.
authors: Liangwen
authors: L
authors: Zhong
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China.
authors: Shuang
authors: S
authors: Dou
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China.
authors: Jun
authors: J
authors: Wang
authors: Department of Cell Biology, Nanjing Medical University, Nanjing 210029, China.
authors: Jianmin
authors: J
authors: Li
authors: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors: Mingwei
authors: M
authors: Wang
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China qshi@ustc.edu.cn meiyide@ustc.edu.cn wumian@ustc.edu.cn.
authors: Qinghua
authors: Q
authors: Shi
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China qshi@ustc.edu.cn meiyide@ustc.edu.cn wumian@ustc.edu.cn.
authors: Yide
authors: Y
authors: Mei
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China qshi@ustc.edu.cn meiyide@ustc.edu.cn wumian@ustc.edu.cn.
authors: Mian
authors: M
authors: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001723:Birth Rate; D002460:Cell Line; D016384:Consensus Sequence; D015536:Down-Regulation; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D010641:Phenotype; D034622:RNA Interference; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D018398:Homeodomain Proteins; D053605:Leukemia Inhibitory Factor; D035683:MicroRNAs; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C552691:MIRN18 microRNA, mouse
keywords: Emx2; LIF; embryo implantation; miR-181