title: Restricted dog leucocyte antigen (DLA) class II haplotypes and genotypes in Beagles.
abstract: Beagles are commonly used in vaccine trials as part of the regulatory approval process. Genetic restriction within this breed and the impact this might have on vaccine responses are rarely considered. This study was designed to characterise diversity of dog leucocyte antigen (DLA) class II genes in a breeding colony of laboratory Beagles, whose offspring are used in vaccine studies. DLA haplotypes were determined by PCR and sequence-based typing from genomic DNA extracted from blood. Breeding colony Beagles had significantly different DLA haplotype frequencies in comparison with pet Beagles and both groups showed limited DLA diversity. Restricted DLA class II genetic variability within Beagles might result in selective antigen presentation and vaccine responses that are not necessarily representative of those seen in other dog breeds.
authors: Department of Pathology and Pathogen Biology, Royal Veterinary College, North Mymms, Hertfordshire AL9 7TA, United Kingdom. Electronic address: fsoutter@rvc.ac.uk.
authors: Francesca
authors: F
authors: Soutter
authors: Centre for Integrated Genomic Medical Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.
authors: Lorna J
authors: LJ
authors: Kennedy
authors: Centre for Integrated Genomic Medical Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.
authors: William E R
authors: WE
authors: Ollier
authors: Departament de Medicina i Cirurgia Animal, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors: Laia
authors: L
authors: Solano-Gallego
authors: Department of Pathology and Pathogen Biology, Royal Veterinary College, North Mymms, Hertfordshire AL9 7TA, United Kingdom.
authors: Brian
authors: B
authors: Catchpole
mesh_terms: D000818:Animals; D004285:Dogs / Q000235:genetics* / Q000276:immunology*; D005838:Genotype; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D006649:Histocompatibility Antigens
keywords: Beagle; Dog leucocyte antigen; Major histocompatibility complex
references: Tissue Antigens. 2002 Mar;59(3):194-204
references: 12074709
references: Tissue Antigens. 2002 Jul;60(1):43-52
references: 12366782
references: J Hered. 2003 Jan-Feb;94(1):23-6
references: 12692158
references: Nat Genet. 1997 Sep;17(1):11-2
references: 9288086
references: Science. 1999 Mar 12;283(5408):1748-52
references: 10073943
references: Nature. 2005 Dec 8;438(7069):803-19
references: 16341006
references: Tissue Antigens. 2007 Apr;69 Suppl 1:272-88
references: 17445218
references: Vaccine. 2007 Dec 12;25(51):8500-7
references: 18006120
references: Genetics. 2008 May;179(1):593-601
references: 18493074