title: Evaluation of immunoturbidimetric albumin reagent from Diagam on c502/Cobas8000® analyzer: comparison with immunonephelometry and colorimetric methods.
abstract: BACKGROUND
  Until now, specific proteins have traditionally been analyzed by immunonephelometry requiring spe- cific equipment. Due to laboratory consolidation, turbidimetry is commonly used for the determination of specific proteins. Biochemistry analyzers have a wide range of turbidimetric assays available to a single workstation, which makes them particularly efficient. For our laboratory consolidation, we installed two distinct Cobas8000® modular analyzer series. However, the turbidimetric albumin from Roche did not give satisfactory results in re- gard to the traceability to the reference measurement material.
  
  
  METHODS
  Analytical assays of turbidimetric albumin from Diagam (Liège, Belgique) on c502/Cobas8000®, includ- ing imprecision studies, accuracy using the ERM®-DA470k/IFCC, and correlation with nephelometry and colo- rimetry, were performed.
  
  
  RESULTS
  Total precision for the immunoturbidimetric assays was consistently better than 2.3% CV and linear throughout the dynamic range of the assays. Correlation of serum albumin by turbidimetry is in good agreement with nephelometry. The mean ± SD of the reference material ERM-DA470k was 37.6 ± 0.25 g/L, with a bias of 1.07%.
  
  
  CONCLUSIONS
  Turbidimetric albumin from Diagam meets the requirements of accuracy and precision for optimal clinical use and could be an alternative without the associated cost of a dedicated instrument in the context of lab- oratory consolidation.
authors: Marie
authors: M
authors: Dupuy
authors: Marion
authors: M
authors: Almeras
authors: Stephanie
authors: S
authors: Badiou
authors: Anne Sophie
authors: AS
authors: Bargnoux
authors: Jean Paul
authors: JP
authors: Cristol
mesh_terms: D015415:Biomarkers / Q000097:blood; D003124:Colorimetry / Q000592:standards; D004867:Equipment Design; D006801:Humans; D006934:Hypercalcemia / Q000097:blood; D006996:Hypocalcemia / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000592:standards; D011237:Predictive Value of Tests; D012015:Reference Standards; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D015415:Biomarkers; D012709:Serum Albumin