title: The Escherichia coli O157:H7 cattle immunoproteome includes outer membrane protein A (OmpA), a modulator of adherence to bovine rectoanal junction squamous epithelial (RSE) cells.
abstract: Building on previous studies, we defined the repertoire of proteins comprising the immunoproteome (IP) of Escherichia coli O157:H7 (O157) cultured in DMEM supplemented with norepinephrine (O157 IP), a β-adrenergic hormone that regulates E. coli O157 gene expression in the gastrointestinal tract, using a variation of a novel proteomics-based platform proteome mining tool for antigen discovery, called "proteomics-based expression library screening" (PELS; Kudva et al., 2006). The E. coli O157 IP (O157-IP) comprised 91 proteins, and included those identified previously using proteomics-based expression library screening, and also proteins comprising DMEM and bovine rumen fluid proteomes. Outer membrane protein A (OmpA), a common component of the above proteomes, and reportedly a contributor to E. coli O157 adherence to cultured HEp-2 epithelial cells, was interestingly found to be a modulator rather than a contributor to E. coli O157 adherence to bovine rectoanal junction squamous epithelial cells. Our results point to a role for yet to be identified members of the O157-IP in E. coli O157 adherence to rectoanal junction squamous epithelial cells, and additionally implicate a possible role for the outer membrane protein A regulator, TdcA, in the expression of such adhesins. Our observations have implications for the development of efficacious vaccines for preventing E. coli O157 colonization of the bovine gastrointestinal tract.
authors: Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA, USA.
authors: Indira T
authors: IT
authors: Kudva
authors: Harvard Partners Center For Genetics and Genomics, Cambridge, MA, USA.
authors: Bryan
authors: B
authors: Krastins
authors: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.|Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
authors: Alfredo G
authors: AG
authors: Torres
authors: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
authors: Robert W
authors: RW
authors: Griffin
authors: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA.
authors: Haiqing
authors: H
authors: Sheng
authors: Harvard Partners Center For Genetics and Genomics, Cambridge, MA, USA.
authors: David A
authors: DA
authors: Sarracino
authors: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA.
authors: Carolyn J
authors: CJ
authors: Hovde
authors: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.|Department of Medicine, Harvard Medical School, Boston, MA, USA.|Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA.
authors: Stephen B
authors: SB
authors: Calderwood
authors: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.|Department of Medicine, Harvard Medical School, Boston, MA, USA.|Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA.
authors: Manohar
authors: M
authors: John
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D007106:Immune Sera / Q000737:chemistry; D009638:Norepinephrine / Q000494:pharmacology; D012417:Rumen / Q000166:cytology / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D007106:Immune Sera; C083450:TdcA protein, E coli; D015534:Trans-Activators; C078167:OMPA outer membrane proteins; D009638:Norepinephrine
keywords: Cattle; GeLC-MS/MS; Immunoproteome; Norepinephrine; O157
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