title: Analysis on the arcelin expression in bruchid pest resistant wild pulses using real time RT-qPCR.
abstract: Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to. increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, Lablab purpureus. The results revealed different levels of arcelin gene expression in the tested varieties. Canavalia virosa registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.
authors: Shanmugavel
authors: S
authors: Sakthivelkumar
authors: Velayutham
authors: V
authors: Veeramani
authors: Karuppiah
authors: K
authors: Hilda
authors: Munusamy
authors: M
authors: Arumugam
authors: Sundaram
authors: S
authors: Janarthanan
mesh_terms: D000818:Animals; D032449:Canavalia / Q000235:genetics / Q000469:parasitology; D001517:Coleoptera / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics; D007887:Fabaceae / Q000145:classification / Q000235:genetics* / Q000469:parasitology; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics*; D006790:Host-Parasite Interactions; D027805:Phaseolus / Q000235:genetics / Q000469:parasitology; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000235:genetics* / Q000469:parasitology; D013045:Species Specificity
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins