title: Standard reporting requirements for biological samples in metabolomics experiments: microbial and in vitro biology experiments.
abstract: With the increasing use of metabolomics as a means to study a large number of different biological research questions, there is a need for a minimal set of reporting standards that allow the scientific community to evaluate, understand, repeat, compare and re-investigate metabolomics studies. Here we propose, a first draft of minimal requirements to effectively describe the biological context of metabolomics studies that involve microbial or in vitro biological subjects. This recommendation has been produced by the microbiology and in vitro biology working subgroup of the Metabolomics Standards Initiative in collaboration with the yeast systems biology network as part of a wider standardization initiative led by the Metabolomics Society. Microbial and in vitro biology metabolomics is defined by this sub-working group as studies with any cell or organism that require a defined external medium to facilitate growth and propagation. Both a minimal set and a best practice set of reporting standards for metabolomics experiments have been defined. The minimal set of reporting standards for microbial or in vitro biology metabolomics experiments includes those factors that arespecificfor metabolomics experiments and that critically determine the outcome of the experiments. The best practice set of reporting standards contains both the factors that arespecificfor metabolomics experiments andgeneral aspectsthat critically determine the outcome of any microbial or in vitro biological experiment.
authors: Department of Microbiology, TNO Quality of Life, P.O. Box 360, Zeist, The Netherlands.
authors: Mariët J
authors: MJ
authors: van der Werf
authors: Degussa, Hanau-Wolfgang, Germany.
authors: Ralf
authors: R
authors: Takors
authors: Center for Microbial Biotechnology, Technical University of Denmark, Lyngby, Denmark.
authors: Jørn
authors: J
authors: Smedsgaard
authors: Center for Microbial Biotechnology, Technical University of Denmark, Lyngby, Denmark.
authors: Jens
authors: J
authors: Nielsen
authors: Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia.
authors: Tom
authors: T
authors: Ferenci
authors: Biosystems adn Process engineering laboratory, INSA Toulouse, Toulouse, France.
authors: Jean Charles
authors: JC
authors: Portais
authors: Systesm Biotechnology Group, Saarland University, Saarbruecken, Germany.
authors: Christoph
authors: C
authors: Wittmann
authors: School of Biological Sciences, University of Wales, Bangor, UK.
authors: Mark
authors: M
authors: Hooks
authors: Scientific Department, Sigma Tau, Rome, Italy.
authors: Alberta
authors: A
authors: Tomassini
authors: Fermentation Technology Group, Forschungszentrum Jülich, Julich, Germany.
authors: Marco
authors: M
authors: Oldiges
authors: National Institute for Environmental Health Science, Research Triangle Park, NC USA.
authors: Jennifer
authors: J
authors: Fostel
authors: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors: Uwe
authors: U
authors: Sauer
keywords: In vitro biology, Metabolomics; Microbiology; Minimal reporting standards; Sample context
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