title: Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis.
abstract: Molecular approaches have been investigated to overcome difficulties in identification and differentiation of Brucella spp. using conventional phenotypic methods. In this study, high-resolution melt (HRM) analysis was used for rapid identification and differentiation of members of Brucella genus. A total of 41 Brucella spp. isolates from human brucellosis were subjected to HRM analysis using 4 sets of primers, which identified 40 isolates as Brucella melitensis and 1 as Brucella canis. The technique utilized low DNA concentration and was highly reproducible. The assay is shown to be a useful diagnostic tool, which can rapidly differentiate Brucella up to species level.
authors: Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Electronic address: jama@imr.gov.my.
authors: Jama'ayah
authors: J
authors: Mohamed Zahidi
authors: Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors: Tay
authors: T
authors: Bee Yong
authors: Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors: Rohaidah
authors: R
authors: Hashim
authors: Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors: Azura
authors: A
authors: Mohd Noor
authors: Microbiology Unit, Department of Pathology, Hospital Pulau Pinang, Jalan Residensi, 10990 Georgetown, Pulau Pinang, Malaysia.
authors: Siti Hawa
authors: SH
authors: Hamzah
authors: Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors: Norazah
authors: N
authors: Ahmad
mesh_terms: D041002:Brucella canis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017347:Brucella melitensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008296:Malaysia; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D044366:Transition Temperature
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial
keywords: Brucella; Brucellosis; HRM analysis; PCR in Brucella; Real-time PCR Brucella spp