title: Antioxidant activity stimulated by ultraviolet radiation in the nervous system of a crustacean.
abstract: Ultraviolet (UV) radiation can produce biological damage, principally oxidative stress, by increasing the production of reactive oxygen species (ROS). This study evaluated biochemical impairments related to the oxidative stress induced by UVA, UVB and UVA+UVB (solar simulator-SIM) in environmental doses, during five consecutive days of exposure, in the brain and eyestalk of the crab Ucides cordatus. We evaluated these regions by sampling on the 1st, 3rd and 5th days of UV exposure for lipid peroxidation (LPO), antioxidant capacity against the peroxyl radical (ACAP), and the activities of catalase (CAT), glutathione peroxidase (GPX) and glutathione-S-transferase (GST). Immunohistochemical and immunoblotting assays were performed for anti-activated-caspase 3 in the brains. After the first day of exposure, LPO increased in the eyestalks and brains of the UV-exposed animals; ACAP, and CAT, GPX and GST activities also increased in the brains. On the third day, the LPO values in the eyestalk remained high in the UV-exposed groups, while ACAP decreased in the brain and eyestalk and CAT activity remained high in all irradiated groups in both regions. On the fifth day, LPO decreased in the eyestalk and brain of the UV-exposed groups. These results may have been a consequence of the antioxidant defense system (ADS) activity, since CAT activity was high in both regions, ACAP was high in the eyestalks of the SIM group, and GPX activity remained high in the eyestalks of the UVA and UVB groups. Immunohistochemical assays and immunoblotting showed that there was apoptosis in the brains of the UV-exposed crabs. In conclusion, environmental doses of UV can cause oxidative damage to the CNS cells, including apoptosis.
authors: Programa de Pós Graduação em Ciências Biológicas-Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ 21941-590, Brazil. Electronic address: gabrielahollmann@biof.ufrj.br.
authors: Gabriela
authors: G
authors: Hollmann
authors: Programa de Pós Graduação em Ciências Biológicas-Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ 21941-590, Brazil. Electronic address: gabi_ferreiira@hotmail.com.
authors: Gabrielle de Jesus
authors: Gde J
authors: Ferreira
authors: Programa de Pós Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG, Rio Grande, RS 96201-900, Brazil. Electronic address: geihs@hotmail.com.
authors: Márcio Alberto
authors: MA
authors: Geihs
authors: Programa de Pós Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG, Rio Grande, RS 96201-900, Brazil. Electronic address: biovargas@gmail.com.
authors: Marcelo Alves
authors: MA
authors: Vargas
authors: Programa de Pós Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG, Rio Grande, RS 96201-900, Brazil. Electronic address: famnery@terra.com.br.
authors: Luiz Eduardo Maia
authors: LE
authors: Nery
authors: Programa de Pós Graduação em Ciências Biológicas-Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ 21941-590, Brazil. Electronic address: acleitao@biof.ufrj.br.
authors: Álvaro
authors: Á
authors: Leitão
authors: Programa de Pós Graduação em Ciências Biológicas-Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ 21941-590, Brazil. Electronic address: rlinden@biof.ufrj.br.
authors: Rafael
authors: R
authors: Linden
authors: Programa de Pós Graduação em Ciências Biológicas-Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ 21941-590, Brazil. Electronic address: sallodi@biof.ufrj.br.
authors: Silvana
authors: S
authors: Allodi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D003386:Brachyura / Q000528:radiation effects*; D004789:Enzyme Activation / Q000187:drug effects; D015227:Lipid Peroxidation / Q000528:radiation effects; D009420:Nervous System / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013472:Sunlight; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; D010088:Oxidoreductases
keywords: Apoptosis; Central Nervous System; Crustacean; Oxidative stress; Ultraviolet radiation