title: In the Solanaceae, a hierarchy of bHLHs confer distinct target specificity to the anthocyanin regulatory complex.
abstract: The anthocyanin biosynthetic pathway is regulated by a transcription factor complex consisting of an R2R3 MYB, a bHLH, and a WD40. Although R2R3 MYBs belonging to the anthocyanin-activating class have been identified in many plants, and their role well elucidated, the subgroups of bHLH implicated in anthocyanin regulation seem to be more complex. It is not clear whether these potential bHLH partners are biologically interchangeable with redundant functions, or even if heterodimers are involved. In this study, AcMYB110, an R2R3 MYB isolated from kiwifruit (Actinidia sp.) showing a strong activation of the anthocyanin pathway in tobacco (Nicotiana tabacum) was used to examine the function of interacting endogenous bHLH partners. Constitutive expression of AcMYB110 in tobacco leaves revealed different roles for two bHLHs, NtAN1 and NtJAF13. A hierarchical mechanism is shown to control the regulation of transcription factors and consequently of the anthocyanin biosynthetic pathway. Here, a model is proposed for the regulation of the anthocyanin pathway in Solanaceous plants in which AN1 is directly involved in the activation of the biosynthetic genes, whereas JAF13 is involved in the regulation of AN1 transcription.
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand.
authors: Mirco
authors: M
authors: Montefiori
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand.
authors: Cyril
authors: C
authors: Brendolise
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand.
authors: Andrew P
authors: AP
authors: Dare
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand.
authors: Kui
authors: K
authors: Lin-Wang
authors: The New Zealand Institute for Plant and Food Research Ltd, Private Bag 11 600, Palmerston North, New Zealand.
authors: Kevin M
authors: KM
authors: Davies
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand Biochemistry Department, School of Medical Sciences, University of Otago, Dunedin 9054, New Zealand Centre for Tropical Crops and Biocommodities Queensland University of Technology Brisbane, Queensland, Australia.
authors: Roger P
authors: RP
authors: Hellens
authors: The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92 169, Auckland, New Zealand School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand andrew.allan@plantandfood.co.nz.
authors: Andrew C
authors: AC
authors: Allan
mesh_terms: D029042:Actinidia / Q000235:genetics; D000872:Anthocyanins / Q000096:biosynthesis*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D051792:Basic Helix-Loop-Helix Proteins; D010940:Plant Proteins; D014157:Transcription Factors
keywords: MYB; Solanaceae; bHLH; tobacco; transcription factors.; transcriptional control
references: Plant Cell. 2002 Sep;14(9):2121-35
references: 12215510
references: Science. 2004 May 14;304(5673):982
references: 15143274
references: Planta. 2009 Sep;230(4):747-54
references: 19621239
references: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13579-84
references: 11095727
references: Planta. 2011 Aug;234(2):363-75
references: 21484270
references: Plant Cell. 2006 May;18(5):1274-91
references: 16603655
references: Plant Cell. 2000 Oct;12(10):1863-78
references: 11041882
references: BMC Plant Biol. 2010 Mar 21;10:50
references: 20302676
references: Genetics. 1996 Dec;144(4):1425-36
references: 8978031
references: Plant J. 2011 Mar;65(5):771-84
references: 21235651
references: Plant J. 2006 Jun;46(5):768-79
references: 16709193
references: Plant Physiol. 2001 Jun;126(2):485-93
references: 11402179
references: Plant Physiol. 2010 Jul;153(3):1398-412
references: 20472752
references: Plant J. 2011 Apr;66(1):94-116
references: 21443626
references: Planta. 2010 Apr;231(5):1061-76
references: 20157728
references: New Phytol. 2013 Apr;198(1):59-70
references: 23398515
references: Plant J. 2005 Apr;42(2):218-35
references: 15807784
references: PLoS One. 2010 Oct 11;5(10):e13230
references: 20949001
references: Plant J. 1998 Feb;13(4):475-88
references: 9680994
references: Plant Cell. 2000 Sep;12(9):1619-32
references: 11006336
references: Plant Methods. 2005 Dec 18;1:13
references: 16359558
references: EMBO J. 1999 Oct 1;18(19):5370-9
references: 10508169
references: Plant Cell. 1989 Dec;1(12):1175-83
references: 2535537
references: Proc Natl Acad Sci U S A. 2012 Jul 24;109(30):E2091-7
references: 22778424
references: Syst Biol. 2003 Oct;52(5):696-704
references: 14530136
references: Plant Physiol. 2006 Nov;142(3):1216-32
references: 17012405
references: Plant Cell. 2006 Apr;18(4):831-51
references: 16531495
references: Plant Cell. 1999 Aug;11(8):1433-44
references: 10449578
references: Mol Plant. 2010 May;3(3):509-23
references: 20118183
references: Plant J. 2004 Oct;40(1):22-34
references: 15361138
references: Plant Methods. 2008 Jul 07;4:17
references: 18601751
references: Genes Dev. 1997 Jun 1;11(11):1422-34
references: 9192870
references: J Exp Bot. 2011 May;62(8):2465-83
references: 21278228
references: Plant J. 2007 Feb;49(3):414-27
references: 17181777
references: Nucleic Acids Res. 1999 Jan 1;27(1):297-300
references: 9847208
references: J Agric Food Chem. 2012 Oct 24;60(42):10546-54
references: 23013475
references: Mol Biol Evol. 2010 Apr;27(4):862-74
references: 19942615
references: BMC Genomics. 2013 Jan 16;14:28
references: 23324587
references: Funct Plant Biol. 2012 Sep;39(8):619-638
references: 32480814
references: Plant J. 1991 Jul;1(1):37-49
references: 1844879
references: EMBO J. 1987 Dec 1;6(12):3553-8
references: 3428265