title: Enteropathogens detected in a daycare center, Southeastern Brazil: bacteria, virus, and parasite research.
abstract: BACKGROUND
  The objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center.
  
  
  METHODS
  From October 2010 to February 2011 stool samples from 100 children enrolled in a government daycare center in the municipality of São José do Rio Preto, in the state of São Paulo, were collected and analyzed.
  
  
  RESULTS
  A total of 246 bacteria were isolated in 99% of the fecal samples; 129 were in the diarrheal group and 117 in the non-diarrheal group. Seventy-three strains of Escherichia coli were isolated, 19 of Enterobacter, one of Alcaligenes and one of Proteus. There were 14 cases of mixed colonization with Enterobacter and E. coli. Norovirus and Astrovirus were detected in children with clinical signs suggestive of diarrhea. These viruses were detected exclusively among children residing in urban areas. All fecal samples were negative for the presence of the rotavirus species A and C. The presence of Giardia lamblia, Entamoeba coli, Endolimax nana and hookworm was observed. A significant association was found between food consumption outside home and daycare center and the presence of intestinal parasites.
  
  
  CONCLUSIONS
  For children of this daycare center, intestinal infection due to pathogens does not seem to have contributed to the occurrence of diarrhea or other intestinal symptoms. The observed differences may be due to the wide diversity of geographical, social and economic characteristics and the climate of Brazil, all of which have been reported as critical factors in the modulation of the frequency of different enteropathogens.
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Edna Donizetti Rossi
authors: ED
authors: Castro
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Marcela Cristina Braga Yassaka
authors: MC
authors: Germini
authors: Virology Section, Evandro Chagas Institute, Ananindeua, Pará, Brazil.
authors: Joana D'Arc Pereira
authors: JD
authors: Mascarenhas
authors: Virology Section, Evandro Chagas Institute, Ananindeua, Pará, Brazil.
authors: Yvone Benchimol
authors: YB
authors: Gabbay
authors: Virology Section, Evandro Chagas Institute, Ananindeua, Pará, Brazil.
authors: Ian Carlos Gomes
authors: IC
authors: de Lima
authors: Virology Section, Evandro Chagas Institute, Ananindeua, Pará, Brazil.
authors: Patrícia dos Santos
authors: Pdos S
authors: Lobo
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Valéria Daltibari
authors: VD
authors: Fraga
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Luciana Moran
authors: LM
authors: Conceição
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Ricardo Luiz Dantas
authors: RL
authors: Machado
authors: Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
authors: Andréa Regina Baptista
authors: AR
authors: Rossit
mesh_terms: D001938:Brazil / Q000453:epidemiology; D002656:Child Day Care Centers; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology; D005243:Feces / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology* / Q000469:parasitology* / Q000821:virology; D007411:Intestinal Diseases, Parasitic / Q000209:etiology; D012959:Socioeconomic Factors; D014505:Urban Population
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