title: Contrasting Patterns of Agonist-induced Store-operated Ca2+ Entry and Vasoconstriction in Mesenteric Arteries and Aorta With Aging.
abstract: Ca is a crucial factor in the regulation of smooth muscle contraction. Store-operated Ca entry (SOCE) is one pathway that mediates Ca influx and smooth muscle contraction. Vessel contraction function usually alters with aging to cause severe vascular-related diseases. However, the underlying mechanism is still not fully understood. Here, we assessed intracellular Ca and vessel tension and found that SOCE and SOCE-mediated contraction of vascular smooth muscle cells (VSMCs) was reduced in aorta but increased in mesenteric arteries from aged rats. The results of Western blot and immunofluorescence staining show that the expression levels of Orai1, a store-operated Ca channel, were increased in VSMCs of mesenteric arteries but were reduced in VSMCs of aorta with aging. In conclusion, we demonstrated that the changing pattern of SOCE and SOCE-mediated contraction of VSMCs is completely reversed in mesenteric arteries and aorta with aging, providing a potential therapeutic target for clinical treatment in age-related vascular diseases.
authors: *Department of Surgery, The First Affiliated Hospital, Hefei, China; and †Department of Physiology, Anhui Medical University, Hefei, China.
authors: Yong
authors: Y
authors: Yang
authors: Jinhang
authors: J
authors: Zhu
authors: Xia
authors: X
authors: Wang
authors: Nairui
authors: N
authors: Xue
authors: Juan
authors: J
authors: Du
authors: Xiangling
authors: X
authors: Meng
authors: Bing
authors: B
authors: Shen
mesh_terms: D000367:Age Factors; D000375:Aging / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000819:agonists* / Q000378:metabolism; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000378:metabolism; D000071740:ORAI1 Protein; D018384:Oxidative Stress; D017207:Rats, Sprague-Dawley; D000071737:Stromal Interaction Molecule 1; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D002120:Calcium Channel Agonists; D015220:Calcium Channels; D008562:Membrane Glycoproteins; D000071740:ORAI1 Protein; C532493:Orai1 protein, rat; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D014662:Vasoconstrictor Agents; D013482:Superoxide Dismutase
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