title: Preliminary study on the immunogenicity of a newly developed GCC Tdap vaccine and its protection efficacy against Bordetella pertussis in a murine intranasal challenge model.
abstract: OBJECTIVE
  Active reduced dose tetanus-diphtheria-acellular pertussis (Tdap) vaccination for adolescents and adults is necessary because waning immunity after primary diphtheria-tetanus-pertussis vaccination is related to the recent emergence of pertussis. This study was conducted to compare the immunogenicity and protection efficacy against Bordetella pertussis between a new GCC Tdap vaccine and a commercially available Tdap vaccine in a murine model.
  
  
  METHODS
  BALB/c mice were immunized with two doses of diphtheria-tetanus-acellular pertussis (DTaP) vaccine for priming and a subsequent Tdap booster vaccination. According to the type of booster vaccine, mice were divided into four groups: commercially available Tdap vaccine in group 1 and GCC Tdap vaccines of different combinations of pertussis antigens in groups 2 to 4. Humoral and cell-mediated immune responses and protection efficacy using a murine intranasal challenge model after booster vaccination were compared among the four groups.
  
  
  RESULTS
  Every group showed significant increases in antibody titers against pertussis antigens such as pertussis toxin, filamentous hemagglutinin, and pertactin after booster vaccination. Spleen cells showed both Th1 and Th2 cell-mediated immune responses stimulated by pertussis antigens in all groups without any significant difference. In the intranasal B. pertussis infection model, bacteria were eradicated in all groups five days after challenge infection.
  
  
  CONCLUSIONS
  This preliminary study did not show significantly different immunogenicity or protection efficacy of the new GCC Tdap vaccines compared to the commercially available Tdap vaccine, although a more extensive study is necessary to assess the differing efficacies of the new GCC Tdap vaccines.
authors: Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Seung Beom
authors: 0000-0002-1299-2137
authors: SB
authors: Han
authors: The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Kyu Ri
authors: 0000-0001-9135-1890
authors: KR
authors: Kang
authors: The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Dong Ho
authors: 0000-0001-5395-7197
authors: DH
authors: Huh
authors: The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Hee Chul
authors: 0000-0002-5715-7143
authors: HC
authors: Lee
authors: Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Soo Young
authors: 0000-0002-5354-3135
authors: SY
authors: Lee
authors: Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Jong-Hyun
authors: 0000-0001-8641-7904
authors: JH
authors: Kim
authors: Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Jae Kyun
authors: 0000-0002-3978-5787
authors: JK
authors: Hur
authors: Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; The Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Jin Han
authors: 0000-0003-1610-6742
authors: JH
authors: Kang
keywords: Diphtheria-tetanus-acellular pertussis vaccine; Efficacy; Immunogenicity; Mice; Republic of Korea
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