title: Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development.
abstract: In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
authors: Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
authors: Jie-Bi
authors: JB
authors: Hu
authors: Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
authors: Ting-Ru
authors: TR
authors: Chen
authors: 1] Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan [2] Institute of Molecular Science, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
authors: Yu-Chie
authors: YC
authors: Chen
authors: 1] Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan [2] Institute of Molecular Science, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
authors: Pawel L
authors: PL
authors: Urban
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000465:Algorithms; D001081:Apyrase / Q000378:metabolism; D004581:Electronics / Q000295:instrumentation; D055616:Microtechnology / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation
chemical_list: D000255:Adenosine Triphosphate; D001081:Apyrase
references: Biotechnol Bioeng. 1999;66(3):180-8
references: 10577472
references: Talanta. 2013 Nov 15;116:514-9
references: 24148438
references: J Bacteriol. 1971 Dec;108(3):1072-86
references: 4333317
references: Nat Rev Genet. 2005 Jul;6(7):533-43
references: 15995697
references: Bioorg Med Chem Lett. 2005 Sep 1;15(17):3860-4
references: 15990297
references: Nature. 2005 Nov 24;438(7067):449-53
references: 16306983
references: Mol Syst Biol. 2006;2:2006.0028
references: 16738572
references: J Med Chem. 2008 Apr 24;51(8):2372-86
references: 18363348
references: Photochem Photobiol Sci. 2008 Sep;7(9):1085-90
references: 18754056
references: Nat Rev Genet. 2010 May;11(5):367-79
references: 20395970
references: J Photochem Photobiol B. 2010 Oct 5;101(1):1-8
references: 20655239
references: Nat Chem Biol. 2011 May;7(5):271-7
references: 21423171
references: Anal Biochem. 2011 Oct 1;417(1):36-40
references: 21693101
references: Sensors (Basel). 2013;13(5):5528-41
references: 23698265
references: Science. 2004 Jan 9;303(5655):158-61
references: 14715986