title: Upregulation of MicroRNA-126 Contributes to Endothelial Progenitor Cell Function in Deep Vein Thrombosis via Its Target PIK3R2.
abstract: Deep vein thrombosis (DVT) is a common complication of surgery. Endothelial progenitor cells (EPCs) are recruited into resolving venous thrombi. In this report, we investigated the effects of miR-126 on EPCs function and venous thrombus resolution. We demonstrated that overexpression of miR-126 enhanced EPCs' migration and tubulogenic activity in vitro, and promoted EPCs' homing and thrombus resolving in vivo. Moreover, we identified that miR-126 directly targeted PIK3R2 and affected PI3K/Akt signaling axis. Overall, our findings demonstrated that miR-126 promoted EPCs function through suppressing PIK3R2 expression and modulation of miR-126 may represent a potential therapeutic intervention for treating DVT.
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Qingyou
authors: Q
authors: Meng
authors: Department of General Surgery, the Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
authors: Wenbin
authors: W
authors: Wang
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Xiaobin
authors: X
authors: Yu
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Wendong
authors: W
authors: Li
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Lingshang
authors: L
authors: Kong
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Aimin
authors: A
authors: Qian
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Chenglong
authors: C
authors: Li
authors: Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China.
authors: Xiaoqiang
authors: X
authors: Li
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002465:Cell Movement; D058543:Class Ia Phosphatidylinositol 3-Kinase; D004195:Disease Models, Animal; D066026:Endothelial Progenitor Cells / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D015854:Up-Regulation; D020246:Venous Thrombosis / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C571458:MIRN126 microRNA, rat; D035683:MicroRNAs; C000606306:PIK3R2 protein, rat; D019869:Phosphatidylinositol 3-Kinases; D058543:Class Ia Phosphatidylinositol 3-Kinase
keywords: EPCs; HOMING; MIGRATION; THROMBUS RESOLUTION; miR-126