title: Useful immunohistochemical indicators in canine mast cell tumours.
abstract: Morphological and immunohistochemical analysis of 45 canine mast cell tumours was performed to determine whether the proteins examined are useful for a more precise description of tumour morphology and a more reliable determination of the prognosis in patients. Tissue sections were stained according to the standard haematoxylin and eosin (HE) technique and with toluidine blue to demonstrate cytoplasmic granules. Immunohistochemical studies were performed, using the cell markers CD117 (c-kit), p16 and von Willebrand factor (FVIII). In CD117 three different staining patterns were observed: (1) membranous reaction, (2) intense staining of cytoplasm, and (3) a diffuse, delicate cytoplasmic reaction. Von Willebrand antibody was evaluated on the basis of the number of blood vessels stained. p16 expression was evaluated by scoring positive nuclear reaction. Positive expression was demonstrated for all examined antigens, but their level of expression differed depending on the grades of tumour malignancy. Statistical analysis of the results documented a pronounced positive correlation between the markers studied and the grade of tumour malignancy (P < 0.001). It was shown that each of the cell markers examined represents a useful prognostic indicator for patients with mast cell tumours. The calculated correlation coefficients demonstrate a strong association between the expressions of CD117, FVIII and p16, and the histological malignancy of a tumour.
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Małgorzata
authors: M
authors: Kandefer-Gola
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Marcin
authors: M
authors: Nowak
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Janusz A
authors: JA
authors: Madej
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Stanisław
authors: S
authors: Dzimira
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Rafal
authors: R
authors: Ciaputa
authors: Wroclaw University of Environmental and Life Sciences Department of Pathology, Faculty of Veterinary Medicine ul. C. K. Norwida 31 50-375 Wroclaw Poland.
authors: Izabela
authors: I
authors: Janus
keywords: CD117; FVIII; Mast cell tumour; dog; p16