title: Toll-like receptor 4 gene Asp299Gly and Thr399Ile polymorphisms in type 2 diabetes mellitus: a meta-analysis of 15,059 subjects.
abstract: OBJECTIVE
  Toll-like receptor 4 (TLR4) gene Asp299Gly and Thr399Ile polymorphisms have been reported to be associated with susceptibility to type 2 diabetes mellitus (T2DM) with inconsistent results. In an effort to clarify earlier inconclusive results, a meta-analysis evaluating the precise associations between TLR4 gene Asp299Gly and Thr399Ile polymorphisms and T2DM risk was performed.
  
  
  METHODS
  We searched the PubMed, Embase, Web of Science, CBMdisc, CNKI, and Google Scholar until July 17, 2014. Additionally, hand searching of the references of identified articles were performed. Original observational studies dealing with the associations between TLR4 gene Asp299Gly and Thr399Ile polymorphisms and T2DM risk were selected. Heterogeneity and publication bias were determined and the meta-analysis was performed by Review Manager 5.1.4 and Stata 11.0.
  
  
  RESULTS
  Seventeen articles involving 25 studies were included in the final meta-analysis, covering a total of 5963 T2DM cases and 9096 controls. For TLR4 gene Asp299Gly polymorphism, 17 studies were combined showing no evidence for association between TLR4 gene Asp299Gly polymorphism and T2DM risk. For TLR4 gene Thr399Ile polymorphism, eight studies were combined. There was also lack of evidence for significant association between TLR4 gene Thr399Ile polymorphism and T2DM risk. In addition, the similar results were obtained in the sensitivity analyses.
  
  
  CONCLUSIONS
  The present meta-analysis indicates that TLR4 gene Asp299Gly and Thr399Ile polymorphisms are not associated with increased T2DM risk.
authors: Department of Emergency, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Haidian District, Beijing 100142, China. Electronic address: shuaishuaijita@sohu.com.
authors: Yan-Wei
authors: YW
authors: Yin
authors: Department of Emergency, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Haidian District, Beijing 100142, China.
authors: Qi
authors: Q
authors: Wang
authors: Jinsong Sanatorium of Beijing Air Force, Beijing 100021, China.
authors: Qian-Qian
authors: QQ
authors: Sun
authors: Department of Emergency, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Haidian District, Beijing 100142, China.
authors: Ai-Min
authors: AM
authors: Hu
authors: Department of Emergency, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Haidian District, Beijing 100142, China.
authors: Hong-Li
authors: HL
authors: Liu
mesh_terms: D019943:Amino Acid Substitution; D001224:Aspartic Acid / Q000235:genetics; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005998:Glycine / Q000235:genetics; D006801:Humans; D007532:Isoleucine / Q000235:genetics; D008957:Models, Genetic; D064887:Observational Studies as Topic / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide; D013912:Threonine / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D007532:Isoleucine; D013912:Threonine; D001224:Aspartic Acid; D005998:Glycine
keywords: Meta-analysis; Polymorphism; Toll-like receptor 4; Type 2 diabetes mellitus