title: InstantLabs® Salmonella species detection method: matrix extension.
abstract: The performance of InstantLabs® Salmonella Species Food Safety Kit to detect Salmonella in four food matrixes was validated against the International Organization for Standardization (ISO) reference method 6579:2002. The matrixes (raw ground beef, raw chicken breast, raw ground chicken, and lettuce) were inoculated with low levels of Salmonella (<1 CFU/test portion) to generate fractional positives (5-15) in 20 inoculated samples. These matrixes were co-inoculated with Escherichia coli O157:H7 at two to five times the level of Salmonella. Samples were validated using 375 g (meat) or 25 g (lettuce and poultry) test portions enriched in FASTGRO TM SE at 42±1 °C for 12 h and 10 h, respectively. All samples were confirmed using the ISO reference method, regardless of initial-screen result. The InstantLabs test method was shown to perform as well as or better than the reference method for the detection of Salmonella species in ground beef, chicken breast, ground chicken, and lettuce. Inclusivity and exclusivity testing revealed no false negatives among the 100 Salmonella serovars and no false positives among the 30 non-Salmonella species examined, respectively.
authors: Neil
authors: N
authors: Sharma
authors: Lauren
authors: L
authors: Bambusch
authors: Thu
authors: T
authors: Le
authors: Amit
authors: A
authors: Morey
authors: Melinda
authors: M
authors: Hayman
authors: Sergio J
authors: SJ
authors: Montez
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation; D002417:Cattle; D002645:Chickens; D019453:Escherichia coli O157 / Q000302:isolation & purification; D005516:Food Microbiology / Q000295:instrumentation*; D018545:Lactuca / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation; D012475:Salmonella / Q000302:isolation & purification*