title: TYLCV-Is movement in planta does not require V2 protein.
abstract: Tomato yellow leaf curl virus (TYLCV), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. V2 mutants of TYLCV-Is and related viruses tend to induce symptomless infection with attenuated viral DNA levels, while accumulating close to wild-type DNA levels in protoplasts, suggesting V2 as a movement protein. The discovery of plant-silencing mechanisms and viral silencing suppressors, V2 included, led us to reconsider V2׳s involvement in viral movement. We studied two mutant versions of the virus, one impaired in V2 silencing-suppression activity, and another carrying a non-translatable V2. While both mutant viruses spread in the infected plant to newly emerged leaves at the same rate as the wild-type virus, their DNA-accumulation levels were tenfold lower than in the wild-type virus. Thus, we suggest that the setback in virus proliferation, previously ascribed to a movement impediment, is due to lack of silencing-suppression activity.
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel; Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel.
authors: Hagit
authors: H
authors: Hak
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors: Yael
authors: Y
authors: Levy
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors: Sam A
authors: SA
authors: Chandran
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors: Eduard
authors: E
authors: Belausov
authors: Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel.
authors: Abraham
authors: A
authors: Loyter
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors: Moshe
authors: M
authors: Lapidot
authors: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. Electronic address: ygafni@volcani.agri.gov.il.
authors: Yedidya
authors: Y
authors: Gafni
mesh_terms: D053839:Begomovirus / Q000235:genetics / Q000502:physiology*; D055786:Gene Knockout Techniques; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D053830:Plant Viral Movement Proteins
keywords: Movement protein; Suppressor of RNA silencing; TYLCV