title: Microbial diversity and abundance in the Xinjiang Luliang long-term water-flooding petroleum reservoir.
abstract: Microbial populations associated with microbial enhanced oil recovery (MEOR) and their abundance in the Xinjiang Luliang water-flooding petroleum reservoir were investigated using 16S rRNA, nitrate reductases, dissimilatory sulfate reductase, and methyl coenzyme-M reductase-encoded genes to provide ecological information for the potential application of MEOR. 16S rRNA gene miseq sequencing revealed that this reservoir harbored large amounts of taxa, including 155 bacterial and 7 archeal genera. Among them, Arcobacter, Halomonas, Marinobacterium, Marinobacter, Sphingomonas, Rhodococcus, Pseudomonas, Dietzia, Ochrobactrum, Hyphomonas, Acinetobacter, and Shewanella were dominant, and have the potential to grow using hydrocarbons as carbon sources. Metabolic gene clone libraries indicated that the nitrate-reducing bacteria (NRB) mainly belonged to Pseudomonas, Azospirillum, Bradyrhizobium, Thauera, Magnetospirillum, Sinorhizobium, Azoarcus, and Rhodobacter; the sulfate-reducing bacteria (SRB) were Desulfarculus, Desulfomonile, Desulfosarcina, Desulfotignum, Desulfacinum, Desulfatibacillum, Desulfatibacillum, Desulfomicrobium, and Desulfovibrio; while the methanogens were archaea and belonged to Methanomethylovorans, Methanosaeta, Methanococcus, Methanolobus, and Methanobacterium. Real-time quantitative PCR analysis indicated that the number of bacterial 16S rRNA reached 106copies/mL, while the metabolic genes of NRB, SRB, and methanogens reached 104copies/mL. These results show that the Luliang reservoir has abundant microbial populations associated with oil recovery, suggesting that the reservoir has potential for MEOR.
authors: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China.|College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors: Peike
authors: P
authors: Gao
authors: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China.|College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors: Huimei
authors: H
authors: Tian
authors: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China.|College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors: Guoqiang
authors: G
authors: Li
authors: College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, China.
authors: Hongwen
authors: H
authors: Sun
authors: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China.|College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors: Ting
authors: T
authors: Ma
keywords: 16S rRNA; MEOR; QPCR; microbial community; miseq
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