title: Regulation of ICAM-1 in cells of the monocyte/macrophage system in microgravity.
abstract: Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte/macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Katrin
authors: K
authors: Paulsen
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Svantje
authors: S
authors: Tauber
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Flow Cytometry Facility, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Claudia
authors: C
authors: Dumrese
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Gesine
authors: G
authors: Bradacs
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Dana M
authors: DM
authors: Simmet
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Nadine
authors: N
authors: Gölz
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Swantje
authors: S
authors: Hauschild
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Christiane
authors: C
authors: Raig
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Stephanie
authors: S
authors: Engeli
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Annett
authors: A
authors: Gutewort
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Eva
authors: E
authors: Hürlimann
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Josefine
authors: J
authors: Biskup
authors: Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Felix
authors: 0000-0003-3418-2650
authors: F
authors: Unverdorben
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Gabriela
authors: G
authors: Rieder
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Daniel
authors: D
authors: Hofmänner
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Lisa
authors: L
authors: Mutschler
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Sonja
authors: S
authors: Krammer
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Isabell
authors: 0000-0003-2119-9648
authors: I
authors: Buttron
authors: German Aerospace Center, Space Agency, Königswinterer Straße 522-524, 53227 Bonn, Germany.
authors: Claudia
authors: C
authors: Philpot
authors: Integrated Functional Genomics (IFG), University of Muenster, Roentgenstraß 21, 48149 Muenster, Germany.
authors: Andreas
authors: A
authors: Huge
authors: KEK GmbH, Kemberger Straße 5, 06905 Bad Schmiedeberg, Germany.
authors: Hartwin
authors: H
authors: Lier
authors: University of Applied Science Jena, Carl-Zeiss-Promenade 2, 07745 Jena, Germany.
authors: Ines
authors: I
authors: Barz
authors: KEK GmbH, Kemberger Straße 5, 06905 Bad Schmiedeberg, Germany ; University of Applied Science Jena, Carl-Zeiss-Promenade 2, 07745 Jena, Germany.
authors: Frank
authors: F
authors: Engelmann
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland.
authors: Liliana E
authors: LE
authors: Layer
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Cora S
authors: CS
authors: Thiel
authors: Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany ; Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland ; Study Group "Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen" (MARS), Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
authors: Oliver
authors: 0000-0002-4721-1038
authors: O
authors: Ullrich
mesh_terms: D000818:Animals; D016923:Cell Death; D002454:Cell Differentiation; D015536:Down-Regulation; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000166:cytology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012399:Rotation; D013026:Space Flight; D020298:U937 Cells; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1
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