title: Spatially-resolved exploration of the mouse brain glycome by tissue glyco-capture (TGC) and nano-LC/MS.
abstract: Tissue glyco-capture (TGC), a highly sensitive MS-compatible method for extraction of glycans from tissue, was combined with structure-specific nano-LC/MS for sensitive and detailed profiling of the mouse brain glycome. Hundreds of glycan structures were directly detected by accurate mass MS and structurally elucidated by MS/MS, revealing the presence of novel glycan motifs such as antennary fucosylation, sulfation, and glucuronidation that are potentially associated with cellular signaling and adhesion. Microgram-level sensitivity enabled glycomic analysis of specific regions of the brain, as demonstrated on not only brain sections (with a one-dimensional spatial resolution of 20 μm) but also isolated brain structures (e.g., the hippocampus). Reproducibility was extraordinarily high (R > 0.98) for both method and instrumental replicates. The pairing of TGC with structure-specific nano-LC/MS was found to be an exceptionally powerful platform for qualitative and quantitative exploration of the brain glycome.
authors: Asia Glycomics Reference Site , Daejeon 305-764, Korea.
authors: In Jung
authors: IJ
authors: Ji
authors: Serenus
authors: S
authors: Hua
authors: Dong Hee
authors: DH
authors: Shin
authors: Nari
authors: N
authors: Seo
authors: Jae Yun
authors: JY
authors: Hwang
authors: Ik-Soon
authors: IS
authors: Jang
authors: Myoung-Goo
authors: MG
authors: Kang
authors: Jong-Soon
authors: JS
authors: Choi
authors: Hyun Joo
authors: HJ
authors: An
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036103:Nanotechnology / Q000379:methods; D011134:Polysaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D011134:Polysaccharides