title: Sequence analysis of Indian iris yellow spot virus ambisense genome segments: evidence of interspecies RNA recombination.
abstract: The nucleotide sequence of M- and S-RNA segments of an Indian iris yellow spot virus (IYSV) were determined. Sequence comparisons showed that both of these sequences shared less than 95 % identity with those other known IYSV isolates. Phylogenetic analysis revealed that the S- and M-RNA sequences of known IYSV isolates clustered with those of the tospoviruses, tomato yellow ring virus, polygonum ringspot virus and hippeastrum chlorotic ringspot virus. Further, multiple recombination detection methods detected inter- and intra-species recombination events that clustered primarily within the intergenic regions of S- and M-RNA, suggesting that these are possibly recombination hotspots in IYSV and closely related tospoviruses.
authors: ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, 410505, India, sureshgawande76@gmail.com.
authors: S J
authors: SJ
authors: Gawande
authors: V S
authors: VS
authors: Gurav
authors: A A
authors: AA
authors: Ingle
authors: D P
authors: DP
authors: Martin
authors: R
authors: R
authors: Asokan
authors: J
authors: J
authors: Gopal
mesh_terms: D016000:Cluster Analysis; D027621:Iridaceae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017865:Tospovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral