title: Qualitative characterization of the rat liver mitochondrial lipidome using all ion fragmentation on an Exactive benchtop Orbitrap MS.
abstract: Untargeted lipidomics profiling by liquid chromatography-mass spectrometry (LC-MS) allows researchers to observe the occurrences of lipids in a biological sample without showing intentional bias to any specific class of lipids and allows retrospective reanalysis of data collected. Typically, and in the specific method described, a general extraction method followed by LC separation is used to achieve nonspecific class coverage of the lipidome prior to high-resolution accurate mass (HRAM) MS detection. Here we describe a workflow including the isolation of mitochondria from liver tissue, followed by mitochondrial lipid extraction and the LC-MS conditions used for data acquisition. We also highlight how, in this method, all-ion fragmentation can be used to identify species of lower abundances, often missed by data-dependent fragmentation techniques. Here we describe the isolation of mitochondria from liver tissue, followed by mitochondrial lipid extraction and the LC-MS conditions used for data acquisition.
authors: Thermo Fisher Scientific, Cambridge, MA, 02139, USA.
authors: Susan S
authors: SS
authors: Bird
authors: Irina G
authors: IG
authors: Stavrovskaya
authors: Rose M
authors: RM
authors: Gathungu
authors: Fateme
authors: F
authors: Tousi
authors: Bruce S
authors: BS
authors: Kristal
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats
references: Prog Lipid Res. 2013 Oct;52(4):590-614
references: 24007978
references: Nat Chem Biol. 2005 Sep;1(4):223-32
references: 16408039
references: Anal Chem. 2011 Feb 1;83(3):940-9
references: 21192696
references: J Lipid Res. 2013 Oct;54(10):2623-35
references: 23690505
references: Biochim Biophys Acta. 2009 Oct;1788(10):2048-58
references: 19539604
references: J Cell Biol. 2011 Jan 10;192(1):7-16
references: 21220505
references: Free Radic Biol Med. 1991;11(1):81-128
references: 1937131
references: Anal Chem. 2011 Sep 1;83(17):6648-57
references: 21774539
references: Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):1020-7
references: 18515061
references: Physiol Rev. 2007 Jan;87(1):99-163
references: 17237344
references: J Mass Spectrom. 2006 Mar;41(3):372-89
references: 16498600
references: J Exp Med. 2004 Jul 19;200(2):211-22
references: 15263028
references: Free Radic Biol Med. 2010 Aug 15;49(4):567-79
references: 20472054
references: J Biol Chem. 1996 Mar 15;271(11):6033-8
references: 8626387
references: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W606-12
references: 17584797
references: Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3698-703
references: 19234110
references: Nucleic Acids Res. 2009 Jan;37(Database issue):D603-10
references: 18953024
references: J Biol Chem. 2003 Dec 26;278(52):52873-80
references: 14561769
references: Apoptosis. 2007 May;12(5):815-33
references: 17294078
references: Anal Chem. 2013 Jan 15;85(2):1114-23
references: 23210743
references: Trends Biochem Sci. 2012 Jan;37(1):32-41
references: 22014644
references: Ther Drug Monit. 2005 Dec;27(6):747-51
references: 16404815
references: Nature. 2013 Feb 14;494(7436):266-70
references: 23334424
references: J Lipid Res. 2005 May;46(5):839-61
references: 15722563
references: Rev Neurosci. 1998;9(2):105-16
references: 9711902