title: Development of a peptide nucleic acid-based multiprobe real-time PCR method targeting the hsp65 gene for differentiation among Mycobacterium abscessus strains.
abstract: Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense).
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors: Kijeong
authors: K
authors: Kim
authors: Department of Microbiology and Immunology, Biomedical Sciences, Cancer Research Institute, and Liver Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Byoung-Jun
authors: BJ
authors: Kim
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea.
authors: Tae Sun
authors: TS
authors: Shim
authors: Department of Microbiology and Immunology, Biomedical Sciences, Cancer Research Institute, and Liver Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Seok-Hyun
authors: SH
authors: Hong
authors: Department of Microbiology and Immunology, Biomedical Sciences, Cancer Research Institute, and Liver Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Yoon-Hoh
authors: YH
authors: Kook
authors: Department of Microbiology and Immunology, Biomedical Sciences, Cancer Research Institute, and Liver Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea kbumjoon@snu.ac.kr.
authors: Bum-Joon
authors: BJ
authors: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005838:Genotype; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D020135:Peptide Nucleic Acids; C074119:heat-shock protein 65, Mycobacterium
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