title: Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.
abstract: The ability to move DNA between Staphylococcus strains is essential for the genetic manipulation of this bacterium. Often in the Staphylococci, this is accomplished through transduction using generalized transducing phage and can be performed in different ways and therefore the presence of two transduction procedures in this book. The following protocol is a relatively easy-to-perform, broth-based procedure that we have used extensively to move both plasmids and chromosomal fragments between strains of Staphylococcus aureus.
authors: Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.
authors: Kelsey L
authors: KL
authors: Krausz
authors: Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA. jbose@kumc.edu.
authors: Jeffrey L
authors: JL
authors: Bose
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D013203:Staphylococcal Infections / Q000235:genetics* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*
keywords: Bacteriophage; Transduction; phi11; phi80α