title: Time-resolved spectrometry of mitochondrial NAD(P)H fluorescence and its applications for evaluating the oxidative state in living cells.
abstract: Time-resolved fluorescence spectrometry is a highly valuable technological tool to detect and characterize mitochondrial metabolic oxidative changes by means of endogenous fluorescence (Chorvat and Chorvatova, Laser Phys Lett 6: 175-193, 2009). Here, we describe the detection and measurement of endogenous mitochondrial NAD(P)H (nicotinamide adenine dinucleotide (phosphate)) fluorescence directly in living cultured cells using fluorescence lifetime spectrometry imaging after excitation with 405 nm picosecond (ps) laser. Time-correlated single photon counting (TCSPC) method is employed.
authors: Department of Biophotonics, International Laser Center, Ilkovičova 3, 84104, Bratislava, Slovakia.
authors: Julia
authors: J
authors: Horilova
authors: Hauke
authors: H
authors: Studier
authors: Zuzana
authors: Z
authors: Nadova
authors: Pavol
authors: P
authors: Miskovsky
authors: Dusan
authors: D
authors: Chorvat
authors: Alzbeta Marcek
authors: AM
authors: Chorvatova
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D004579:Electron Transport; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D009249:NADP