title: [Effect of high glucose on migration of BMSCs through inhibiting CXCR-4].
abstract: OBJECTIVE
  To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4.
  
  
  METHODS
  Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis.
  
  
  RESULTS
  The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2.
  
  
  CONCLUSIONS
  High glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 pathway.
authors: Stomatological Center, General Hospital of PLA. Beijing 100853, China.E-mail:hugo_zhang_005@163.com.
authors: Bo
authors: B
authors: Zhang
authors: Na
authors: N
authors: Liu
authors: Bin
authors: B
authors: Gu
authors: Hao
authors: H
authors: Wu
authors: Yu-xuan
authors: YX
authors: Gao
authors: Dong-sheng
authors: DS
authors: Wang
authors: Hong-chen
authors: HC
authors: Liu
mesh_terms: D000818:Animals; D002465:Cell Movement; D054377:Chemokine CXCL12; D005947:Glucose; D059630:Mesenchymal Stem Cells; D051381:Rats; D017208:Rats, Wistar; D019718:Receptors, CXCR4
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D005947:Glucose