title: Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction.
abstract: OBJECTIVE
  To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.
  
  
  METHODS
  Sixteen male rats were allocated across four groups: LG--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.
  
  
  RESULTS
  There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.
  
  
  CONCLUSIONS
  Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats.
authors: School of Medicine, Brasilia University, Brazil.
authors: Hugo Muscelli
authors: HM
authors: Alecrim
authors: School of Medicine, Brasilia University, Brazil.
authors: Sérgio Andurte Carvalho
authors: SA
authors: Duarte
authors: School of Medicine, UnB, Brasília, DF, Brazil.
authors: Maria Eduarda Bonavides
authors: ME
authors: Amaral
authors: School of Medicine, UnB, Brasília, DF, Brazil.
authors: Filipe
authors: F
authors: Diógenis
authors: School of Medicine, UnB, Brasília, DF, Brazil.
authors: Fabiana Pirani
authors: FP
authors: Carneiro
authors: School of Medicine, UnB, Brasília, DF, Brazil.
authors: João Batista de
authors: JB
authors: Sousa
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000097:blood; D001651:Cholestasis, Extrahepatic / Q000601:surgery*; D003106:Colon / Q000187:drug effects / Q000293:injuries* / Q000601:surgery; D003135:Common Bile Duct / Q000601:surgery; D019587:Dietary Supplements; D005973:Glutamine / Q000494:pharmacology*; D041781:Jaundice, Obstructive / Q000503:physiopathology*; D008026:Ligation; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013718:Tensile Strength / Q000187:drug effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D005973:Glutamine; D001663:Bilirubin