title: Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats.
abstract: An increasing amount of evidence demonstrates the anti-aging effect of Heshouwu in pill form. In this study, a subacute aging rat model was established by continuous intraperitoneal injection of D-galactose and treated with Heshouwu decoction (a Chinese herb for tonifying the kidney, comprising Heshouwu pill, Herba Epimedii, Radix Salviae Miltiorrhiae, and Poria). Heshouwu pill treated rats were the positive control group. Radioimmunoassay, immunohistochemical staining, and western blot assay showed hypothalamic gonadotropin-releasing hormone, hypothalamic substance P, and serum gonadotropin levels to be significantly increased in the model rats; the concentrations of hypothalamic β-endorphin, and serum levels of insulin-like growth factor 1 and testosterone were significantly decreased. 17β- and 3β-hydroxysteroid dehydrogenase expression in testicular tissue was also decreased. Intragastric administration of Heshouwu decoction at high (9.6 g/mL/100 g), medium (4.8 g/mL/100 g), and low (2.4 g/mL/100 g) doses, Heshouwu decoction pretreatment at a medium dose (4.8 g/mL/100 g), and Heshouwu pill (2.06 g/mL/100 g) significantly reversed these changes. Heshouwu decoction pretreatment and high-dose Heshouwu decoction had the greatest anti-aging effects. These experimental findings indicate that Heshouwu decoction can improve hypothalamic-pituitary-testicular axis secretion in a subacute aging rat model, and prevent and delay gonadal axis aging, with an effect superior to that of Heshouwu pill.
authors: School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China.
authors: Siyun
authors: S
authors: Niu
authors: School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China.
authors: Suru
authors: S
authors: Kou
authors: Institute of Basic Medical Sciences, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Xiaochun
authors: X
authors: Zhou
authors: School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China.
authors: Liang
authors: L
authors: Ding
keywords: Heshouwu decoction; aging; gonadotropin; gonadotropin-releasing hormone; hydroxysteroid dehydrogenase; hypothalamic-pituitary-testicular axis; neural regeneration; pituitary gonadal axis
references: Trends Endocrinol Metab. 2011 Nov;22(11):437-42
references: 21852148
references: Am J Physiol. 1997 Oct;273(4 Pt 2):R1407-13
references: 9362306
references: Mech Ageing Dev. 1999 May 17;108(3):239-51
references: 10405984
references: Mol Cell Endocrinol. 2007 Feb;265-266:131-7
references: 17207923
references: J Biol Chem. 2006 Nov 17;281(46):35048-56
references: 16968703
references: FEBS Lett. 2007 Aug 21;581(21):4023-8
references: 17673208
references: Clin Exp Pharmacol Physiol. 2001 Apr;28(4):285-9
references: 11251641
references: Exp Gerontol. 1998 Nov-Dec;33(7-8):729-57
references: 9951619
references: J Clin Endocrinol Metab. 1992 Sep;75(3):707-13
references: 1517359
references: Bioorg Med Chem. 2010 Feb;18(3):999-1002
references: 20093034