title: [Impact of JNK inhibitor XG-102 in a diet-induced rat model of non-alcoholic steatohepatitis].
abstract: OBJECTIVE
  To observe the impact of the JNK inhibitor XG-102 in a diet-induced rat model of non-alcoholic steatohepatitis.
  
  
  METHODS
  Forty-eight Sprague-Dawley male rats were subjected to a percutaneous superior mesenteric vein retention catheter operation and fed with a standard diet for 10 days, after which the rats were randomly divided into the following three groups: normal control (NC) group; high-fat (HF) model group; XG-102 treatment group. The HF group was fed an HF diet and treated with 0.9% sodium chloride for 16 weeks. The XG-102 group was fed an HF diet for 16 weeks and simultaneously treated with XG-102 (1 mg/kg) once per day for 4 weeks. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), homeostasis model of assessment insulin resistance (HOMA-IR) and tumor necrosis factor-alpha (TNFa) were measured. Liver histological changes were observed. The protein expressions of phospho-c-Jun and cleaved caspase-3 were detected by western blotting.
  
  
  RESULTS
  Compared with the NC group, the HF group showed significantly higher levels of serum ALT, AST, TC, TG, HOMA-IR and TNFa (P<0.05). Compared with the HF group, the XG-102 group showed significantly lower levels of serum ALT, AST, TC, TG, HOMA-IR and TNFa (P<0.05). The HF group also showed significantly higher protein expression of phospho-c-Jun and cleaved caspase-3 than the NC group (P<0.05) and the XG-102 group (P<0.05).
  
  
  CONCLUSIONS
  The JNK inhibitor XG-102 may ameliorate lipid metabolism, reduce insulin resistance, decrease liver injury and inhibit hepatocytes apoptosis.
authors: Department of Gastroenterology, the pu'ai Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, China.
authors: Yingbin
authors: Y
authors: Hu
authors: Na
authors: N
authors: Peng
authors: Deli
authors: D
authors: Lei
authors: Fangxiong
authors: F
authors: Cheng
authors: Yilian
authors: Y
authors: Chen
mesh_terms: D000410:Alanine Transaminase; D000821:Animal Feed; D000818:Animals; D001219:Aspartate Aminotransferases; D053148:Caspase 3; D002784:Cholesterol; D007333:Insulin Resistance; D050356:Lipid Metabolism; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D010455:Peptides; D047428:Protein Kinase Inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha
chemical_list: D010455:Peptides; D047428:Protein Kinase Inhibitors; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D053148:Caspase 3; C478085:D-JNKI-1