title: MicroRNA-150 Protects Against Pressure Overload-Induced Cardiac Hypertrophy.
abstract: Cardiac hypertrophy is the response of the heart to a variety of hypertrophic stimuli; this condition progresses to heart failure and sudden death. MicroRNAs (miRs) are a family of small, non-coding RNAs that mediate posttranscriptional gene silencing. Recent studies have identified miRs as important regulators in cardiac hypertrophy. One specific miR, miR-150 has been reported to be downregulated in hypertrophic murine hearts. However, the role of miR-150 as a regulator of cardiac hypertrophy remains unclear. In the present study, we used gain-of-function and loss-of-function approaches to investigate the functional roles of miR-150 in cardiac hypertrophy induced by aortic banding. The extent of the cardiac hypertrophy was evaluated by echocardiography and by pathological and molecular analyses of heart samples. Our results revealed that transgenic mice that overexpress miR-150 in the heart were resistant to cardiac hypertrophy and fibrosis through down-regulation of serum response factor (SRF). Conversely, the loss of function of miR-150 by genetic knockdown or antagomiR approaches produced the opposite effects. These studies suggest that miR-150 plays an important role in the regulation of cardiac hypertrophy and SRF is involved in miR-150 mediated anti-hypertrophic effect. Thus, miR-150 may be a new therapeutic target for cardiac hypertrophy.
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Wanli
authors: W
authors: Liu
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Yu
authors: Y
authors: Liu
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Yan
authors: Y
authors: Zhang
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Xueyong
authors: X
authors: Zhu
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Rui
authors: R
authors: Zhang
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Lihua
authors: L
authors: Guan
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Qizhu
authors: Q
authors: Tang
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Hong
authors: H
authors: Jiang
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: Congxin
authors: C
authors: Huang
authors: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.|Cardiovascular Research Institute, Wuhan University, Wuhan, 430060, China.
authors: He
authors: H
authors: Huang
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000235:genetics*; D006332:Cardiomegaly / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D004452:Echocardiography; D005355:Fibrosis / Q000235:genetics / Q000473:pathology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D026362:Serum Response Factor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D035683:MicroRNAs; C529101:Mirn150 microRNA, mouse; D026362:Serum Response Factor
keywords: HYPERTROPHY; MICRORNA; SIGNAL TRANSDUCTION; SRF