title: Human DNA-binding peptidyl-prolyl cis/trans isomerase Par14 is cell cycle dependently expressed and associates with chromatin in vivo.
abstract: BACKGROUND
  Par14, a member of the parvulin family of peptidyl-prolyl cis-trans isomerases that is involved in rRNA processing, microtubule formation and the glucose metabolism and has been suggested to play a role in chromatin remodeling on basis of sequence and structural identities to HMG proteins. Par14 is enriched in the nucleus and binds to double-stranded DNA in vitro.
  
  
  RESULTS
  By means of sub-nuclear biochemical fractionations, we demonstrate that cellular Par14 is associated with chromatin 3-fold higher than with the nuclear matrix in vivo. Par14 is released from the chromatin fraction after treatment with DNase I and elutes at high NaCl concentrations from the nucleic acid-binding fraction. Using qRT-PCR and western blotting we demonstrate that Par14 is up-regulated during the S and G2/M phases in synchronised human foreskin fibroblasts cells.
  
  
  CONCLUSIONS
  In the light of our results, Par14 can be described as an endogenous non-histone chromatin protein, which binds DNA in vivo. We propose that Par14 is involved in a DNA-dependent activity such as transcription.
authors: Department of Structural and Medicinal Biochemistry, Center of Medical Biotechnology, Universität Duisburg-Essen, Room S03 S01 A35, Universitätsstr. 1-5, 45141, Essen, Germany. akuma.saningong@yahoo.com.
authors: Akuma D
authors: AD
authors: Saningong
authors: Department of Structural and Medicinal Biochemistry, Center of Medical Biotechnology, Universität Duisburg-Essen, Room S03 S01 A35, Universitätsstr. 1-5, 45141, Essen, Germany. peter.bayer@uni-due.de.
authors: Peter
authors: P
authors: Bayer
mesh_terms: D000595:Amino Acid Sequence; D002453:Cell Cycle; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D059007:Polytene Chromosomes / Q000378:metabolism; D021381:Protein Transport
chemical_list: D002843:Chromatin; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D004247:DNA; C000606846:PIN1 protein, human; C000606848:PIN4 protein, human; D019696:Peptidylprolyl Isomerase
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