title: Delivery of molecules to the lymph node via lymphatic vessels using ultrasound and nano/microbubbles.
abstract: Lymph node (LN) dissection is the primary option for head and neck cancer when imaging modalities and biopsy confirm metastasis to the sentinel LN. However, there are no effective alternative treatments to dissection for LN metastasis. Here, we describe a novel drug delivery system combining nano/microbubbles (NMBs) with ultrasound (US) that exhibits considerable potential for the delivery of exogenous molecules into LNs through the lymphatic vessels. A solution containing fluorophores (as a model of a therapeutic molecule) and NMBs was injected into the subiliac LNs of MXH10/Mo-lpr/lpr mice, which develop systemic swelling of LNs (up to 13 mm in diameter, similar to human LNs). It was found that the NMBs were delivered to the entire area of the proper axillary LN (proper-ALN) via the lymphatic channels and that these were retained there for more than 8 min. Furthermore, exposure to US in the presence of NMBs enhanced the delivery of fluorophores into the lymphocytes near the lymphatic channels, compared with exposure to US in the absence of NMBs. It is proposed that a system using US and NMBs to deliver therapeutic drugs via lymphatic vessels can serve as a new treatment method for LN metastasis.
authors: Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Miyagi, Japan.
authors: Shigeki
authors: S
authors: Kato
authors: Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Miyagi, Japan.
authors: Yuko
authors: Y
authors: Shirai
authors: Department of Maxillo-Oral Disorders, Tohoku University Hospital, Sendai, Miyagi, Japan.
authors: Hiroyuki
authors: H
authors: Kanzaki
authors: Department of Oral Diagnosis, Tohoku University Hospital, Sendai, Miyagi, Japan.
authors: Maya
authors: M
authors: Sakamoto
authors: Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, Sendai, Miyagi, Japan.
authors: Shiro
authors: S
authors: Mori
authors: Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Miyagi, Japan. Electronic address: Kodama@bme.tohoku.ac.jp.
authors: Tetsuya
authors: T
authors: Kodama
mesh_terms: D000818:Animals; D002214:Capsules / Q000737:chemistry / Q000528:radiation effects*; D019220:High-Energy Shock Waves; D008198:Lymph Nodes / Q000737:chemistry*; D042601:Lymphatic Vessels / Q000737:chemistry*; D051379:Mice; D008815:Mice, Inbred Strains; D053769:Nanocapsules / Q000737:chemistry / Q000528:radiation effects*; D013010:Sonication / Q000379:methods*
chemical_list: D002214:Capsules; D053769:Nanocapsules
keywords: Cavitation; Drug delivery system; Lymph node metastasis; Nano/microbubbles; Ultrasound