title: Associations of matrix metalloproteinase (MMP)-8, MMP-9, and their inhibitor, tissue inhibitor of metalloproteinase-1, with obesity-related biomarkers in apparently healthy adolescent boys.
abstract: OBJECTIVE
  Matrix metalloproteinases (MMPs) have been implicated in atherosclerosis, and therefore, are considered risk factors for metabolic dysfunction in adults. However, there is little data on circulating levels of MMPs and tissue inhibitors of MMPs (TIMPs) with regard to obesity-related biomarkers in the general adolescent population. In the present study, we determined the associations of MMP-8, MMP-9, and TIMP-1 levels and MMP-8/TIMP-1 and MMP-9/TIMP-1 ratios with obesity-related biomarkers in apparently healthy adolescent boys.
  
  
  METHODS
  We measured MMP and TIMP concentrations in plasma samples using the enzyme-linked immunosorbent assay and analyzed their associations with obesity-related biomarkers, such as liver enzymes and lipid profiles, in a sample of 91 Korean boys aged 13-14 years who participated in a general health check-up.
  
  
  RESULTS
  The mean age of the boys was 13.8±0.3 years; 72 boys were normal weight and 19 were overweight/obese. The Pearson correlation coefficients revealed a significant correlation between MMP-8 and aspartate aminotransferase (r=0.217, P=0.039) and alanine aminotransferase (r=0.250, P=0.017) and between TIMP-1 and aspartate aminotransferase (r=0.267, P=0.011). In a multivariate linear regression analysis, serum alanine aminotransferase was positively associated with the MMP-8 level. There were no significant differences in the MMP-8, MMP-9, and TIMP-1 levels or MMP-8/TIMP-1 and MMP-9/TIMP-1 ratios between control and overweight/obese subjects.
  
  
  CONCLUSIONS
  We found a significant association between the MMP-8 level and alanine aminotransferase in the apparently healthy adolescent boys. These findings indicate that there may be a pathophysiological mechanism underlying the relationship between MMP-8 and liver enzymes in young adolescents.
authors: Department of Pediatrics, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, Korea. ; Department of Medicine, The Graduate School, Yonsei University, Seoul, Korea.
authors: Youn Ho
authors: YH
authors: Shin
authors: Department of Pediatrics, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, Korea.
authors: Ki Eun
authors: KE
authors: Kim
authors: Department of Family Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors: Yong-Jae
authors: YJ
authors: Lee
authors: Department of Biotechnology, The Catholic University of Korea, Bucheon St. Mary's Hospital, Bucheon, Korea.
authors: Jae-Hwan
authors: JH
authors: Nam
authors: Department of Pediatrics, Ewha Womans University Mokdong Hospital, Ewha Womans University School of Medicine, Seoul, Korea.
authors: Young Mi
authors: YM
authors: Hong
authors: Department of Pediatrics, National Medical Center, Seoul, Korea.
authors: Hye-Jung
authors: HJ
authors: Shin
keywords: Adolescent; Alanine aminotransferase; Matrix metalloproteinases; Overweight; Tissue inhibitor of metalloproteinase
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