title: Functional characterization of MpaG', the O-methyltransferase involved in the biosynthesis of mycophenolic acid.
abstract: Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa' responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG' gene was functionally and kinetically characterized in vitro. MpaG' catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis.
authors: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101 (China).
authors: Wei
authors: W
authors: Zhang
authors: Shaona
authors: S
authors: Cao
authors: Li
authors: L
authors: Qiu
authors: Fengxia
authors: F
authors: Qi
authors: Zhong
authors: Z
authors: Li
authors: Ying
authors: Y
authors: Yang
authors: Shaohua
authors: S
authors: Huang
authors: Fali
authors: F
authors: Bai
authors: Changning
authors: C
authors: Liu
authors: Xiaobo
authors: X
authors: Wan
authors: Shengying
authors: S
authors: Li
mesh_terms: D053898:Biosynthetic Pathways; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D009173:Mycophenolic Acid / Q000378:metabolism*; D010407:Penicillium / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D008780:Methyltransferases; D009173:Mycophenolic Acid
keywords: biosynthesis; methyltransferases; mycophenolic acid; substrate flexibility; transferases