title: Serum inflammatory factors and circulating immunosuppressive cells are predictive markers for efficacy of radiofrequency ablation in non-small-cell lung cancer.
abstract: In recent years, percutaneous radiofrequency ablation (RFA) has been developed as a new tool in the treatment of non-small-cell lung cancer (NSCLC) in non-surgical patients. There is growing evidence that RFA-mediated necrosis can modulate host immune responses. Here we analysed serum inflammatory factors as well as immunosuppressive cells in the peripheral blood to discover possible prognostic indicators. Peripheral blood and serum samples were collected before RFA and within 3 months after the treatment in a total of 12 patients. Inflammatory cytokines and growth factors were measured in serum by the Bio-Plex assay. Myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs ) were evaluated in the peripheral blood via flow cytometry. In patients developing local or lymphogenic tumour relapse (n=4), we found an early significant increase in the concentration of tumour necrosis factor (TNF)-α as well as chemokine (C-C motif) ligand (CCL)-2 and CCL-4 compared to patients without relapse (n=4) and healthy donors (n=5). These changes were associated with an elevated activity of circulating MDSC indicated by an increased nitric oxide (NO) production in these cells. Elevated serum levels of TNF-α, CCL-2 and CCL-4 associated with an increased NO production in circulating MDSCs might be an early indicator of the incomplete RFA and subsequently a potential tumour relapse in NSCLC.
authors: Department of Thoracic Surgery, St Vincentius Kliniken, Karlsruhe, Germany.
authors: T
authors: T
authors: Schneider
authors: Skin Cancer Unit, German Cancer Research Center, Heidelberg and Department of Dermatology, Venerology and Allergology, University Medical Center Mannheim, Ruprecht-Karl University of Heidelberg, Mannheim, Heidelberg, Germany.
authors: A
authors: A
authors: Sevko
authors: Department of Diagnostic and Interventional Radiology with Nuclear Medicine, Thoraxklinik, Heidelberg University, Heidelberg, Germany.
authors: C P
authors: CP
authors: Heussel
authors: Department of Translational Immunology, German Cancer Research Center, Heidelberg, Germany.
authors: L
authors: L
authors: Umansky
authors: Department of Translational Immunology, German Cancer Research Center, Heidelberg, Germany.
authors: P
authors: P
authors: Beckhove
authors: Department of Thoracic Surgery, Thoraxklinik, Heidelberg University, Heidelberg, Germany.
authors: H
authors: H
authors: Dienemann
authors: Department of Thoracic Surgery, Thoraxklinik, Heidelberg University, Heidelberg, Germany.
authors: S
authors: S
authors: Safi
authors: Skin Cancer Unit, German Cancer Research Center, Heidelberg and Department of Dermatology, Venerology and Allergology, University Medical Center Mannheim, Ruprecht-Karl University of Heidelberg, Mannheim, Heidelberg, Germany.
authors: J
authors: J
authors: Utikal
authors: Department of Thoracic Surgery, Thoraxklinik, Heidelberg University, Heidelberg, Germany.
authors: H
authors: H
authors: Hoffmann
authors: Skin Cancer Unit, German Cancer Research Center, Heidelberg and Department of Dermatology, Venerology and Allergology, University Medical Center Mannheim, Ruprecht-Karl University of Heidelberg, Mannheim, Heidelberg, Germany.
authors: V
authors: V
authors: Umansky
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis / Q000276:immunology* / Q000601:surgery; D017115:Catheter Ablation; D002452:Cell Count; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008175:Lung Neoplasms / Q000097:blood* / Q000276:immunology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology*; D049268:Positron-Emission Tomography; D011379:Prognosis; D014057:Tomography, X-Ray Computed; D016896:Treatment Outcome; D047368:Tumor Burden
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators
keywords: chronic inflammatory factors; myeloid-derived suppressor cells; non-small-cell lung cancer; radiofrequency ablation
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