title: Influence of polymerase brand on microarray-based spoligotyping in low concentrations of mycobacterial DNA.
abstract: Spoligotyping is a widely used typing method for the Mycobacterium tuberculosis complex. Protocols and platforms can be adapted for direct use on patient samples. Serial dilutions of genomic DNA from Mycobacterium bovis BCG strain DSM45071 were spoligotyped by array hybridization using 32 different commercial PCR polymerase preparations. In samples with very low concentrations of mycobacterial DNA, commercially available PCR polymerases differed in their performance, and some yielded no, or false, identification. Direct spoligotyping from samples with very low concentrations of mycobacterial DNA thus requires careful selection of polymerase and strict standardization.
authors: Institute for Medical Microbiology and Hygiene, Technische Universität Dresden, Dresden, Germany; Alere Technologies GmbH, Jena, Germany; Infectognostics Research Campus Jena, Jena, Germany. Electronic address: monecke@rocketmail.com.
authors: Stefan
authors: S
authors: Monecke
authors: Alere Technologies GmbH, Jena, Germany; Infectognostics Research Campus Jena, Jena, Germany.
authors: Ines
authors: I
authors: Engelmann
authors: Alere Technologies GmbH, Jena, Germany; Infectognostics Research Campus Jena, Jena, Germany.
authors: Ralf
authors: R
authors: Ehricht
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000145:classification*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase
keywords: BCG; Mycobacterium tuberculosis complex; Polymerase; Spoligotyping