title: Natural Green coating inhibits adhesion of clinically important bacteria.
abstract: Despite many advances, biomaterial-associated infections continue to be a major clinical problem. In order to minimize bacterial adhesion, material surface modifications are currently being investigated and natural products possess large potential for the design of innovative surface coatings. We report the bioguided phytochemical investigation of Pityrocarpa moniliformis and the characterization of tannins by mass spectrometry. It was demonstrated that B-type linked proanthocyanidins-coated surfaces, here termed Green coatings, reduced Gram-positive bacterial adhesion and supported mammalian cell spreading. The proposed mechanism of bacterial attachment inhibition is based on electrostatic repulsion, high hydrophilicity and the steric hindrance provided by the coating that blocks bacterium-substratum interactions. This work shows the applicability of a prototype Green-coated surface that aims to promote necessary mammalian tissue compatibility, while reducing bacterial colonization.
authors: 1] Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 90610-000, Brazil [2] Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 91501-970, Brazil.
authors: Danielle S
authors: DS
authors: Trentin
authors: Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto/SP, 14040-903, Brazil.
authors: Denise B
authors: DB
authors: Silva
authors: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 90610-000, Brazil.
authors: Amanda P
authors: AP
authors: Frasson
authors: Department of Chemistry, Umeå University, Umeå, SE-90187, Sweden.
authors: Olena
authors: O
authors: Rzhepishevska
authors: Centro de Ciências Biológicas and Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE, 50670-901, Brazil.
authors: Márcia V
authors: MV
authors: da Silva
authors: Center for Biofilm Engineering, Montana State University, Bozeman/MT, 59717, United States of America.
authors: Elinor de L
authors: Ede L
authors: Pulcini
authors: Center for Biofilm Engineering, Montana State University, Bozeman/MT, 59717, United States of America.
authors: Garth
authors: G
authors: James
authors: Instituto de Física, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 91509-900, Brazil.
authors: Gabriel V
authors: GV
authors: Soares
authors: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 90610-000, Brazil.
authors: Tiana
authors: T
authors: Tasca
authors: Department of Chemistry, Umeå University, Umeå, SE-90187, Sweden.
authors: Madeleine
authors: M
authors: Ramstedt
authors: Centro de Ciências da Saúde, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal/RN, 59010-180, Brazil.
authors: Raquel B
authors: RB
authors: Giordani
authors: Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto/SP, 14040-903, Brazil.
authors: Norberto P
authors: NP
authors: Lopes
authors: 1] Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 90610-000, Brazil [2] Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre/RS, 91501-970, Brazil.
authors: Alexandre J
authors: AJ
authors: Macedo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004783:Environmental Microbiology; D004847:Epithelial Cells / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products; D020099:Coated Materials, Biocompatible; D010936:Plant Extracts; D044945:Proanthocyanidins; C013221:proanthocyanidin
references: J Biomed Mater Res. 1998 Dec 5;42(3):425-32
references: 9788506
references: Science. 1987 Sep 25;237(4822):1588-95
references: 3629258
references: Chem Soc Rev. 2006 Sep;35(9):780-9
references: 16936926
references: Microbiology. 2006 Sep;152(Pt 9):2673-82
references: 16946262
references: Mol Nutr Food Res. 2007 Jun;51(6):732-7
references: 17487930
references: Colloids Surf B Biointerfaces. 2007 Sep 1;59(1):35-45
references: 17537618
references: Curr Top Microbiol Immunol. 2008;322:107-31
references: 18453274
references: Langmuir. 2008 Sep 16;24(18):10273-81
references: 18698853
references: Acta Biomater. 2009 Jun;5(5):1399-404
references: 19158003
references: Future Microbiol. 2010 Aug;5(8):1149-51
references: 20722591
references: Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17065-6
references: 20876111
references: Angew Chem Int Ed Engl. 2011 Jan 17;50(3):586-621
references: 21226137
references: Appl Environ Microbiol. 2011 Feb;77(4):1532-5
references: 21169441
references: Acta Biomater. 2011 Apr;7(4):1431-40
references: 21056701
references: Acta Biomater. 2011 May;7(5):2015-28
references: 21194574
references: J Ethnopharmacol. 2011 Sep 1;137(1):327-35
references: 21651970
references: Appl Microbiol Biotechnol. 2012 Jul;95(2):299-311
references: 22618687
references: Arch Intern Med. 2012 Jul 9;172(13):988-96
references: 22777630
references: Adv Drug Deliv Rev. 2012 Sep;64(12):1165-76
references: 22512927
references: Food Chem. 2012 Dec 1;135(3):1485-93
references: 22953884
references: Sci Transl Med. 2012 Sep 26;4(153):153rv10
references: 23019658
references: Langmuir. 2012 Nov 27;28(47):16408-22
references: 23121175
references: Trends Biotechnol. 2013 May;31(5):295-304
references: 23434154
references: Adv Drug Deliv Rev. 2013 Apr;65(4):581-603
references: 22820527
references: PLoS One. 2013;8(6):e66257
references: 23776646
references: Colloids Surf B Biointerfaces. 2013 Oct 1;110:275-80
references: 23732805
references: Biomaterials. 2013 Nov;34(34):8533-54
references: 23953781
references: Nat Rev Drug Discov. 2013 Oct;12(10):791-808
references: 24080700
references: Curr Opin Microbiol. 2013 Oct;16(5):580-9
references: 23880136
references: Trends Biotechnol. 2014 Feb;32(2):82-90
references: 24176168
references: Phytochemistry. 2000 May;54(2):173-81
references: 10872208
references: J Biol Chem. 2000 Aug 25;275(34):26265-76
references: 10818101
references: Nat Prod Rep. 2001 Dec;18(6):641-9
references: 11820762
references: J Agric Food Chem. 2002 Mar 13;50(6):1593-601
references: 11879042
references: Curr Opin Pharmacol. 2003 Oct;3(5):470-3
references: 14559090
references: Nature. 2004 Apr 1;428(6982):487-92
references: 15057821
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Phytochemistry. 2005 Sep;66(18):2281-91
references: 16055161