title: Development and characterization of neutralizing monoclonal antibodies against canine distemper virus hemagglutinin protein.
abstract: Canine distemper virus (CDV) causes a serious multisystemic disease in dogs and other carnivora. Hemagglutinin (H) protein-specific antibodies are mainly responsible for protective immunity against CDV infection. In the present study, six neutralizing MAbs to the H protein of CDV were newly obtained and characterized by immunizing BALB/c mice with a recent Chinese field isolate. Competitive binding inhibition assay revealed that they recognized four distinct antigenic regions of the H protein. Immunofluorescence assay and western blotting showed that all MAbs recognize the conformational rather than the linear epitopes of the H protein. Furthermore, in immunofluorescence and virus neutralization assays, two of the MAbs were found to react only with the recent Chinese field isolate and not with older CDV strains, including vaccine strain Onderstepoort, indicating there are neutralization-related antigenic variations between the recent Chinese field isolate and the older CDV strains examined in this study. The newly established MAbs are useful for differentiating the expanding CDV strains and could be used in immunotherapy and immunodiagnosis against infection with CDV.
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
authors: Zhenwei
authors: Z
authors: Bi
authors: Xingxia
authors: X
authors: Xia
authors: Yongshan
authors: Y
authors: Wang
authors: Yongjie
authors: Y
authors: Mei
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D004216:Distemper / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D004285:Dogs; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006388:Hemagglutinins; D014764:Viral Proteins
keywords: antigenic variation; canine distemper virus; monoclonal antibody; neutralizing activity