title: Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease.
abstract: Cell membrane proteins are believed to play a critical role in the pathogenesis of autoimmune diseases. However, few membrane autoantigens have been linked with Behçet's disease. Here, a cell-chip was performed to identify autoantibody target cells, and the suspected autoantigens were detected using immunoblotting. The amino acid sequences of the detected proteins were determined using LC-MALDI-TOF/TOF. Putative proteins were recombinantly expressed and purified, and a corresponding ELISA was developed and clinically validated using real clinical samples. It was found that a 36-kDa membrane protein--annexin A2--was detected in approximately one-third of the patients' blood circulation. The immunohistochemistry results showed that annexin A2 was highly expressed in vascular endothelial cells. Moreover, vascular involvement was significantly higher in the anti-annexin A2 antibody-positive group versus the anti-annexin A2 antibody-negative group among all the clinical samples analyzed, indicating that annexin A2 is a novel endothelial cell membrane antigen involved in Behçet's disease.
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Peng
authors: P
authors: Chen
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Hai
authors: H
authors: Yan
authors: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China.
authors: Yaping
authors: Y
authors: Tian
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Yiping
authors: Y
authors: Xun
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Lili
authors: L
authors: Shi
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Ran
authors: R
authors: Bao
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Huai
authors: H
authors: Zhang
authors: ImmunoHunt Corporation, 139 Fengtai Rd, Beijing 100071, China.
authors: Guangyu
authors: G
authors: Chen
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Chunhe
authors: C
authors: Yang
authors: Core Facility, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Shutao
authors: S
authors: Sun
authors: 1] Core Laboratory for Clinical Medical Research, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China [2] Department of Clinical Laboratory Diagnosis, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors: Yajie
authors: Y
authors: Wang
authors: Core Laboratory for Clinical Medical Research, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors: Li
authors: L
authors: Liu
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Yabin
authors: Y
authors: Zhou
authors: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China.
authors: Chunyan
authors: C
authors: Zhang
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Xiaoxu
authors: X
authors: Wang
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Yongqiang
authors: Y
authors: Wen
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Yongzhong
authors: Y
authors: Bian
authors: School of Chemistry and Biotechnology Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors: Hongwu
authors: H
authors: Du
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017306:Annexin A2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000032:analysis* / Q000378:metabolism; D001528:Behcet Syndrome / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D018834:Chaperonin 60 / Q000276:immunology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D014469:Umbilical Arteries / Q000378:metabolism / Q000473:pathology; D014471:Umbilical Veins / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
chemical_list: D017306:Annexin A2; D001323:Autoantibodies; D001324:Autoantigens; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins
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