title: Angiomotin functions in HIV-1 assembly and budding.
abstract: Many retroviral Gag proteins contain PPXY late assembly domain motifs that recruit proteins of the NEDD4 E3 ubiquitin ligase family to facilitate virus release. Overexpression of NEDD4L can also stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L may function through an adaptor protein. Here, we demonstrate that the cellular protein Angiomotin (AMOT) can bind both NEDD4L and HIV-1 Gag. HIV-1 release and infectivity are stimulated by AMOT overexpression and inhibited by AMOT depletion, whereas AMOT mutants that cannot bind NEDD4L cannot function in virus release. Electron microscopic analyses revealed that in the absence of AMOT assembling Gag molecules fail to form a fully spherical enveloped particle. Our experiments indicate that AMOT and other motin family members function together with NEDD4L to help complete immature virion assembly prior to ESCRT-mediated virus budding.
authors: Department of Biochemistry, University of Utah, Salt Lake City, United States.
authors: Gaelle
authors: G
authors: Mercenne
authors: Department of Biochemistry, University of Utah, Salt Lake City, United States.
authors: Steven L
authors: SL
authors: Alam
authors: Department of Biochemistry, University of Utah, Salt Lake City, United States.
authors: Jun
authors: J
authors: Arii
authors: Department of Biochemistry, University of Utah, Salt Lake City, United States.
authors: Matthew S
authors: MS
authors: Lalonde
authors: Department of Biochemistry, University of Utah, Salt Lake City, United States.
authors: Wesley I
authors: WI
authors: Sundquist
mesh_terms: D000091082:Angiomotins; D015683:Gene Products, gag / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000502:physiology*; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology*; D008840:Microfilament Proteins; D011485:Protein Binding; D019065:Virus Assembly
chemical_list: C423985:AMOT protein, human; D000091082:Angiomotins; D015683:Gene Products, gag; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins
keywords: ESCRT pathway; HIV Gag protein; NEDD4L; biochemistry; human; infectious disease; microbiology; motin protein family; virus-host interactions; viruses
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