title: A GGCT fluorogenic probe: design, synthesis and application to cancer-related cells.
abstract: Cancer-related γ-glutamyl cyclotransferase (GGCT) specifically converts γ-glutamyl amino acids (γ-Glu-Xaa) into pyroglutamate and the corresponding amino acids (Xaa). Here we report a novel GGCT fluorogenic probe "LISA-101" containing a masked O-acylated fluorophore "resorufin" on the side chain of the P amino acid (Xaa). Upon GGCT treatment, the P amino acid was liberated and spontaneously released the intact fluorophore. Thus, the fluorescence was regained. LISA-101 will expand the strategies for cancer studies.
authors: Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan. t.yoshiya@peptide.co.jp yuji@peptide.co.jp.
authors: Taku
authors: T
authors: Yoshiya
authors: Hiromi
authors: H
authors: Ii
authors: Shugo
authors: S
authors: Tsuda
authors: Susumu
authors: S
authors: Kageyama
authors: Tatsuhiro
authors: T
authors: Yoshiki
authors: Yuji
authors: Y
authors: Nishiuchi
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D009369:Neoplasms / Q000175:diagnosis*; D005722:gamma-Glutamylcyclotransferase / Q000138:chemical synthesis / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D005722:gamma-Glutamylcyclotransferase