title: An effective strategy of magnetic stem cell delivery for spinal cord injury therapy.
abstract: Spinal cord injury (SCI) is a condition that results in significant mortality and morbidity. Treatment of SCI utilizing stem cell transplantation represents a promising therapy. However, current conventional treatments are limited by inefficient delivery strategies of cells into the injured tissue. In this study, we designed a magnetic system and used it to accumulate stem cells labelled with superparamagnetic iron oxide nanoparticles (SPION) at a specific site of a SCI lesion. The loading of stem cells with engineered SPIONs that guarantees sufficient attractive magnetic forces was achieved. Further, the magnetic system allowed rapid guidance of the SPION-labelled cells precisely to the lesion location. Histological analysis of cell distribution throughout the cerebrospinal channel showed a good correlation with the calculated distribution of magnetic forces exerted onto the transplanted cells. The results suggest that focused targeting and fast delivery of stem cells can be achieved using the proposed non-invasive magnetic system. With future implementation the proposed targeting and delivery strategy bears advantages for the treatment of disease requiring fast stem cell transplantation.
authors: Institute of Experimental Medicine, ASCR, 14200 Prague, Czech Republic. sarka.k@biomed.cas.cz.
authors: Dmitry
authors: D
authors: Tukmachev
authors: Oleg
authors: O
authors: Lunov
authors: Vitalii
authors: V
authors: Zablotskii
authors: Alexandr
authors: A
authors: Dejneka
authors: Michal
authors: M
authors: Babic
authors: Eva
authors: E
authors: Syková
authors: Šárka
authors: Š
authors: Kubinová
mesh_terms: D000818:Animals; D052203:Ferrosoferric Oxide / Q000737:chemistry; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D013119:Spinal Cord Injuries / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
chemical_list: D058185:Magnetite Nanoparticles; D052203:Ferrosoferric Oxide