title: Detection of Pseudomonas aeruginosa isolates carrying the blaOXA-142extended-spectrum β-lactamase gene in Taiwan.
abstract: OBJECTIVE
  The emergence of extended-spectrum β-lactamase (ESBL) OXA-142 gene (blaOXA-142) in Pseudomonas aeruginosa has been reported in Bulgaria and other European countries, but rarely in Asia.
  
  
  METHODS
  From January 2009 to December 2012, 90 P. aeruginosa isolates with reduced susceptibility or resistance to ceftazidime (minimum inhibitory concentration ≥ 8 mg/L) were screened for ESBL and other broad-spectrum β-lactamase genes by polymerase chain reaction and sequencing. Clonal relationship of the isolates was determined by pulsed-field gel electrophoresis.
  
  
  RESULTS
  Three isolates were positive for ESBL production, exhibited resistance to ceftazidime, and carried the blaOXA-142gene. The blaOXA-142gene was integrated into class 1 integron. Using Southern blot analysis, blaOXA-142-containing integron was found to be located on a plasmid in all three isolates. Eleven strains of P. aeruginosa carrying blaOXA-17gene were found. The oprD mutation was found in all the 21 ESBL strains of P. aeruginosa.
  
  
  CONCLUSIONS
  This study confirmed the presence of blaOXA-142-positive P. aeruginosa isolates in Taiwan.
authors: Division of Infectious Diseases, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan; Section of Microbiology, Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan; Department of Medicine, Mackay Medical College, Taipei, Taiwan; Graduate Institute of Bioengineering, Tatung University, Taipei, Taiwan.
authors: Chang-Pan
authors: CP
authors: Liu
authors: School of Medicine, National Yang-Ming University, Taipei, Taiwan; Cheng-Hsin General Hospital, Taipei, Taiwan.
authors: Te-Li
authors: TL
authors: Chen
authors: Section of Microbiology, Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
authors: Nai-Yu
authors: NY
authors: Wang
authors: Graduate Institute of Bioengineering, Tatung University, Taipei, Taiwan.
authors: Shan-Fan
authors: SF
authors: Chow
authors: Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, Taipei, Taiwan; National Defense Medical Center, Taipei, Taiwan. Electronic address: jclin@ndmctsgh.edu.tw.
authors: Jung-Chung
authors: JC
authors: Lin
authors: Graduate Institute of Bioengineering, Tatung University, Taipei, Taiwan. Electronic address: tryan@hotmail.com.tw.
authors: Tsong-Rong
authors: TR
authors: Yan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001208:Asia; D002442:Ceftazidime / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000453:epidemiology; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002442:Ceftazidime; D001618:beta-Lactamases
keywords: Extended-spectrum β-lactamase; Integron; OXA-142 gene; Pseudomonas aeruginosa