title: Development of a competitive enzyme-linked immunosorbent assay for detection of antibodies against the 3B protein of foot-and-mouth disease virus.
abstract: Foot-and-mouth disease (FMD) is one of the most highly contagious and economically devastating diseases, and it severely constrains the international trade of animals. Vaccination against FMD is a key element in the control of FMD. However, vaccination of susceptible animals raises critical issues, such as the differentiation of infected animals from vaccinated animals. The current study developed a reliable and rapid test to detect antibodies against the conserved, nonstructural proteins (NSPs) of the FMD virus (FMDV) to distinguish infected animals from vaccinated animals. A monoclonal antibody (MAb) against the FMDV NSP 3B was produced. A competitive enzyme-linked immunosorbent assay (cELISA) for FMDV/NSP antibody detection was developed using a recombinant 3ABC protein as the antigen and the 3B-specific MAb. Sera collected from naive, FMDV experimentally infected, vaccinated carrier, and noncarrier animals were tested using the 3B cELISA. The diagnostic specificity was 99.4% for naive animals (cattle, pigs, and sheep) and 99.7% for vaccinated noncarrier animals. The diagnostic sensitivity was 100% for experimentally inoculated animals and 64% for vaccinated carrier animals. The performance of this 3B cELISA was compared to that of four commercial ELISA kits using a panel of serum samples established by the World Reference Laboratory for FMD at The Pirbright Institute, Pirbright, United Kingdom. The diagnostic sensitivity of the 3B cELISA for the panel of FMDV/NSP-positive bovine serum samples was 94%, which was comparable to or better than that of the commercially available NSP antibody detection kits. This 3B cELISA is a simple, reliable test to detect antibodies against FMDV nonstructural proteins.
authors: National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada.
authors: Ming
authors: M
authors: Yang
authors: The Pirbright Institute, Pirbright, Surrey, United Kingdom.
authors: Satya
authors: S
authors: Parida
authors: National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada.
authors: Tim
authors: T
authors: Salo
authors: National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada.
authors: Kate
authors: K
authors: Hole
authors: Comision Mexico-Estados Unidos para la Prevencion de la Fiebre Aftosa y Otras Enfermedades Exoticas de los Animales, Mexico City, Mexico.
authors: Lauro
authors: L
authors: Velazquez-Salinas
authors: National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada Alfonso.clavijo@ag.tamu.edu.
authors: Alfonso
authors: A
authors: Clavijo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine; D006113:United Kingdom; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: C109754:3ABC protein, virus; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins
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