title: High-performance liquid chromatography method for the determination of hydrogen peroxide present or released in teeth bleaching kits and hair cosmetic products.
abstract: This manuscript presents an HPLC/UV method for the determination of hydrogen peroxide present or released in teeth bleaching products and hair products. The method is based on an oxidation of triphenylphosphine into triphenylphosphine oxide by hydrogen peroxide. Triphenylphosphine oxide formed is quantified by HPLC/UV. Validation data were obtained using the ISO 12787 standard approach, particularly adapted when it is not possible to make reconstituted sample matrices. For comparative purpose, hydrogen peroxide was also determined using ceric sulfate titrimetry for both types of products. For hair products, a cross validation of both ceric titrimetric method and HPLC/UV method using the cosmetic 82/434/EEC directive (official iodometric titration method) was performed. Results obtained for 6 commercialized teeth whitening products and 5 hair products point out similar hydrogen peroxide contain using either the HPLC/UV method or ceric sulfate titrimetric method. For hair products, results were similar to the hydrogen peroxide content using the cosmetic 82/434/EEC directive method and for the HPLC/UV method, mean recoveries obtained on spiked samples, using the ISO 12787 standard, ranges from 100% to 110% with a RSD<3.0%. To assess the analytical method proposed, the HPLC method was used to control 35 teeth bleaching products during a market survey and highlight for 5 products, hydrogen peroxide contents higher than the regulated limit.
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France. Electronic address: pascal.gimeno@ansm.sante.fr.
authors: Pascal
authors: P
authors: Gimeno
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Claudine
authors: C
authors: Bousquet
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Nelly
authors: N
authors: Lassu
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Annie-Françoise
authors: AF
authors: Maggio
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Corinne
authors: C
authors: Civade
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Charlotte
authors: C
authors: Brenier
authors: Agence nationale de sécurité du médicament et des produits de santé (ANSM), Direction des Contrôles (CTROL), 143/147 boulevard Anatole France, 93285 Saint-Denis Cedex, France.
authors: Laurent
authors: L
authors: Lempereur
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000032:analysis* / Q000737:chemistry*; D006203:Hair Preparations / Q000032:analysis* / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D014073:Tooth Bleaching / Q000379:methods; D058205:Tooth Bleaching Agents / Q000032:analysis* / Q000737:chemistry*
chemical_list: D003358:Cosmetics; D006203:Hair Preparations; D058205:Tooth Bleaching Agents; D006861:Hydrogen Peroxide
keywords: Hair products; High performance liquid chromatography/UV detection; Hydrogen peroxide; ISO 12787; Teeth bleaching products