title: High-speed dynamic-clamp interface.
abstract: The dynamic-clamp technique is highly useful for mimicking synaptic or voltage-gated conductances. However, its use remains rare in part because there are few systems, and they can be expensive and difficult for less-experienced programmers to implement. Furthermore, some conductances (such as sodium channels) can be quite rapid or may have complex voltage sensitivity, so high speeds are necessary. To address these issues, we have developed a new interface that uses a common personal computer platform with National Instruments data acquisition and WaveMetrics IGOR to provide a simple user interface. This dynamic clamp implements leak and linear synaptic conductances as well as a voltage-dependent synaptic conductance and kinetic channel conductances based on Hodgkin-Huxley or Markov models. The speed of the system can be assayed using a testing mode, and currently speeds of >100 kHz (10 μs per cycle) are achievable with short latency and little jitter.
authors: Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York;
authors: Yang
authors: Y
authors: Yang
authors: Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York; and.
authors: Timothy
authors: T
authors: Adowski
authors: Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York; and.
authors: Bina
authors: B
authors: Ramamurthy
authors: Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany.
authors: Andreas
authors: A
authors: Neef
authors: Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York; mx@buffalo.edu.
authors: Matthew A
authors: MA
authors: Xu-Friedman
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods; D012984:Software; D012985:Software Design; D014584:User-Computer Interface
keywords: Markov model; WaveMetrics IGOR
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