title: An affinity reagent for the recognition of pyrophosphorylated peptides.
abstract: A resin-bound dinuclear zinc(II) complex for the selective capture of pyrophosphopeptides is reported. The metal complex binds diphosphate esters over other anionic groups, such as monophosphate esters, sulfate esters, and carboxylic acids, with high specificity. Immobilization of the compound provided a reagent capable of binding and retaining nanomolar quantities of pyrophosphopeptide in the presence of cell lysate. The high affinity and specificity of the reagent makes it an attractive tool for the study of in vivo pyrophosphorylation.
authors: Department of Chemistry, Princeton University, Washington Rd, Princeton, NJ 08544 (USA).
authors: John H
authors: JH
authors: Conway
authors: Dorothea
authors: D
authors: Fiedler
mesh_terms: D000345:Affinity Labels; D002477:Cells / Q000737:chemistry; D002846:Chromatography, Affinity; D011756:Diphosphates / Q000737:chemistry*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008958:Models, Molecular; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D010766:Phosphorylation; D013379:Substrate Specificity; D013816:Thermodynamics; D017967:Zinc Compounds / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000345:Affinity Labels; D011756:Diphosphates; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010455:Peptides; D017967:Zinc Compounds
keywords: affinity reagents; proteins; proteomics; pyrophosphorylation; signal transduction