title: Design of a single chambered microbial electrolytic cell reactor for production of biohydrogen from rice straw hydrolysate.
abstract: Rice straw was pretreated using a microwave-assisted alkali pretreatment method. Cellulose recovery was approximately 82 %. This material was hydrolysed in an optimized enzymatic saccharification reaction using cellulase from Lysinibacillus sphaericus. This resulted in saccharification of 49 % of cellulosic biomass into glucose. A single chambered microbial electrolytic cell reactor of volume 2l was built using acrylic plastic sheets with graphite sheet as anode and a stainless-steel mesh as cathode. Shewanella putrefaciens was used as exoelectrogen to oxidize rice straw hydrolysate in the reactor for electrohydrogenesis. The maximum H2 yield obtained was 801 ml H2 g(-1) COD removal. Coulombic efficiency of 88 %, cathodic H2 recovery of 58 % and total H2 recovery of 51 % with an energy efficiency of 74 % were recorded.
authors: Department of Biotechnology, National Institute of Technology-Raipur, Raipur, 492010, Chhattisgarh, India, pguptabiotech@gmail.com.
authors: Pratima
authors: P
authors: Gupta
authors: Piyush
authors: P
authors: Parkhey
mesh_terms: D001406:Bacillaceae / Q000201:enzymology; D001674:Bioelectric Energy Sources; D019149:Bioreactors / Q000382:microbiology*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000302:isolation & purification / Q000378:metabolism*; D004560:Electricity; D006859:Hydrogen / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D020614:Shewanella putrefaciens / Q000378:metabolism*
chemical_list: D006859:Hydrogen; D002482:Cellulose; D002480:Cellulase