title: Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells.
abstract: BACKGROUND
  Curcuma longa Linnaeus and Zingiber officinale Roscoe are two main representatives of Zingiberaceae family studied for a wide range of therapeutic properties, including: antioxidant, anti-inflammatory, anti-angiogenic, antibacterial, analgesic, immunomodulatory, proapoptotic, anti-human immunodeficiency virus properties and anticancer effects. This study was aimed to analyse the ethanolic extracts of Curcuma rhizome (Curcuma longa Linnaeus) and Zingiber rhizome (Zingiber officinale Roscoe) in terms of polyphenols, antioxidant activity and anti-melanoma potential employing the B164A5 murine melanoma cell line.
  
  
  RESULTS
  In order to evaluate the total content of polyphenols we used Folin-Ciocâlteu method. The antioxidant activity of the two ethanolic extracts was determined by DPPH assay, and for the control of antiproliferative effect it was used MTT proliferation assay, DAPI staining and Annexin-FITC-7AAD double staining test. Results showed increased polyphenols amount and antioxidant activity for Curcuma rhizome ethanolic extract. Moreover, 100 μg/ml of ethanolic plant extract from both vegetal products presented in a different manner an antiproliferative, respectively a proapoptotic effect on the selected cell line.
  
  
  CONCLUSIONS
  The study concludes that Curcuma rhizome may be a promising natural source for active compounds against malignant melanoma.
authors: Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. corina.danciu@umft.ro.
authors: Corina
authors: C
authors: Danciu
authors: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Medicine and Pharmacy Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. vlaialavinia@umft.ro.
authors: Lavinia
authors: L
authors: Vlaia
authors: Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Mănăştur Str.,No. 3-5, Cluj-Napoca, 400372, Romania. florinelafetea@yahoo.com.
authors: Florinela
authors: F
authors: Fetea
authors: Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy "Gr.T.Popa", Iasi, Romania. mhancianu@yahoo.com.
authors: Monica
authors: M
authors: Hancianu
authors: Department of Toxicology, Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. dorinacoricovac@umft.ro.
authors: Dorina E
authors: DE
authors: Coricovac
authors: Department of Toxicology, Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. sorina.ciurlea@umft.ro.
authors: Sorina A
authors: SA
authors: Ciurlea
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. codrutasoica@umft.ro.
authors: Codruţa M
authors: CM
authors: Şoica
authors: Faculty of Medicine, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. imarincu@umft.ro.
authors: Iosif
authors: I
authors: Marincu
authors: Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. vlaiav@umft.ro.
authors: Vicentiu
authors: V
authors: Vlaia
authors: Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy "Gr.T.Popa", Iasi, Romania. cadehelean@umft.ro.
authors: Cristina A
authors: CA
authors: Dehelean
authors: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania. trandafirescu.cristina@umft.ro.
authors: Cristina
authors: C
authors: Trandafirescu
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry / Q000145:classification; D020939:Zingiber officinale / Q000737:chemistry; D008546:Melanoma, Experimental / Q000188:drug therapy*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis* / Q000145:classification; D027343:Rhizome / Q000737:chemistry; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
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