title: MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay.
abstract: MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay. MALDI-TOF MS detected activity from 99% of the strains, from all types of carbapenemase (200/202), while Carba-NP assays detected activity from 85% (45/53) of the tested isolates and could not consistently identify OXA- or GES carbapenemase activity.
authors: Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Patrick M
authors: PM
authors: Chong
authors: Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Stuart J
authors: SJ
authors: McCorrister
authors: Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Mark S
authors: MS
authors: Unger
authors: Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: David A
authors: DA
authors: Boyd
authors: Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Michael R
authors: MR
authors: Mulvey
authors: Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada. Electronic address: garrett.westmacott@phac-aspc.gc.ca.
authors: Garrett R
authors: GR
authors: Westmacott
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000201:enzymology*; D013058:Mass Spectrometry; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Acinetobacter; Carbapenemase detection; Enterobacteriaceae; MALDI-TOF; Pseudomonas