title: Evaluation of 5 cleaning and disinfection methods for nets used to collect zebrafish (Danio rerio).
abstract: Few standardized methods of cleaning and disinfecting equipment in zebrafish facilities have been published, even though the effectiveness of these procedures is vital to preventing the transmission of pathogenic organisms. Four chemical disinfectants and rinsing with municipal tap water were evaluated for their ability to disinfect nets used to capture zebrafish. The disinfectants included benzalkonium chloride+methylene blue, sodium hypochlorite, chlorine dioxide, and potassium peroxymonosulfate+sodium chloride for a soak time of 5 or 30 min. Disinfection effectiveness was evaluated by using an ATP-based system that measured the reduction in absolute number and percentage of relative light units. In addition, nets were cultured aerobically on blood and MacConkey agar plates to determine the number of bacteria remaining after disinfection procedures. Soaking nets in sodium hypochlorite for 30 min and in potassium peroxymonosulfate+sodium chloride for 5 or 30 min were effective means of disinfection, according to at least 90% reduction in the number of relative light units and no bacterial growth after cleaning. These results will aid facility managers, veterinarians and investigators in selecting net cleaning and disinfection protocols.
authors: Tri-Institutional Training Program in Laboratory Animal, Medicine and Science, Memorial Sloan-Kettering Cancer Center, The Rockefeller University, and the Weill Cornell Medical College, New York, New York, USA; Division of Comparative Medicine, University of Toronto, Toronto, Ontario, Canada. c.collymore@utoronto.ca.
authors: Chereen
authors: C
authors: Collymore
authors: Memorial Sloan-Kettering Cancer Center and the Weill Cornell, Medical College, New York, New York, USA.
authors: Gina
authors: G
authors: Porelli
authors: Tri-Institutional Training Program in Laboratory Animal, Medicine and Science, Memorial Sloan-Kettering Cancer Center, The Rockefeller University, and the Weill, Cornell Medical College, New York, New York, USA.
authors: Christine
authors: C
authors: Lieggi
authors: Tri-Institutional Training Program in Laboratory Animal, Medicine and Science, Memorial Sloan-Kettering Cancer Center, The Rockefeller University, and the Weill Cornell Medical College, New York, New York, USA; Memorial Sloan-Kettering Cancer Center and the Weill Cornell, Medical College, New York, New York, USA.
authors: Neil S
authors: NS
authors: Lipman
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D017606:Chlorine Compounds / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D010087:Oxides / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D015027:Zebrafish
chemical_list: D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide; D012973:Sodium Hypochlorite
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