title: EHEC Adhesins.
abstract: Adhesins are a group of proteins in enterohemorrhagicEscherichia coli(EHEC) that are involved in the attachment or colonization of this pathogen to abiotic (plastic or steel) and biological surfaces, such as those found in bovine and human intestines. This review provides the most up-to-date information on these essential adhesion factors, summarizing important historical discoveries and analyzing the current and future state of this research. In doing so, the proteins intimin and Tir are discussed in depth, especially regarding their role in the development of attaching and effacing lesions and in EHEC virulence. Further, a series of fimbrial proteins (Lpf1, Lpf2, curli, ECP, F9, ELF, Sfp, HCP, and type 1 fimbriae) are also described, emphasizing their various contributions to adherence and colonization of different surfaces and their potential use as genetic markers in detection and classification of different EHEC serotypes. This review also discusses the role of several autotransporter proteins (EhaA-D, EspP, Saa and Sab, and Cah), as well as other proteins associated with adherence, such as flagella, EibG, Iha, and OmpA. While these proteins have all been studied to varying degrees, all of the adhesins summarized in this chapter have been linked to different stages of the EHEC life cycle, making them good targets for the development of more effective diagnostics and therapeutics.
authors: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, 77555. USA.
authors: Brian D
authors: BD
authors: McWilliams
authors: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, 77555. USA. ; Department of Pathology and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, 77555. USA.
authors: Alfredo G
authors: AG
authors: Torres
references: Microbiol Immunol. 2012 Aug;56(8):513-22
references: 22671942
references: Infect Immun. 2005 Mar;73(3):1735-43
references: 15731074
references: Infect Immun. 2001 Mar;69(3):1444-53
references: 11179311
references: Can J Vet Res. 2011 Apr;75(2):81-8
references: 21731177
references: Mol Microbiol. 2001 Jul;41(2):349-63
references: 11489123
references: Appl Environ Microbiol. 2008 Feb;74(4):1087-93
references: 18083855
references: J Vet Med Sci. 2011 Oct;73(10):1265-73
references: 21628866
references: Infect Immun. 1986 Mar;51(3):953-6
references: 3512443
references: Int J Med Microbiol. 2002 Sep;292(3-4):195-205
references: 12398210
references: Microbes Infect. 2006 Jun;8(7):1741-9
references: 16815722
references: mBio. 2012 Oct 16;3(5):e00280-12
references: 23073764
references: Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):279-83
references: 8552622
references: Appl Environ Microbiol. 2012 Mar;78(6):1752-64
references: 22247152
references: Cell Host Microbe. 2009 Mar 19;5(3):244-58
references: 19286134
references: Infect Immun. 2006 Apr;74(4):2233-44
references: 16552054
references: J Biol Chem. 2011 Dec 9;286(49):42283-42291
references: 22006918
references: Infect Immun. 2009 Aug;77(8):3234-43
references: 19487483
references: Infect Immun. 2009 Sep;77(9):3552-68
references: 19581394
references: Infect Immun. 2004 Oct;72(10):6168-71
references: 15385526
references: J Exp Med. 1998 Nov 16;188(10):1907-16
references: 9815268
references: Infect Immun. 2002 Oct;70(10):5416-27
references: 12228266
references: Mol Microbiol. 2008 May;68(4):838-47
references: 18430080
references: Appl Microbiol Biotechnol. 2008 Apr;78(5):811-9
references: 18256823
references: Environ Microbiol. 2011 May;13(5):1327-41
references: 21352460
references: Infect Immun. 2003 Dec;71(12):7129-39
references: 14638803
references: Infect Immun. 2000 Nov;68(11):6115-26
references: 11035714
references: J Bacteriol. 2005 Dec;187(23):7918-30
references: 16291665
references: J Clin Microbiol. 1998 Mar;36(3):662-8
references: 9508292
references: Infect Immun. 1999 Dec;67(12):6409-17
references: 10569757
references: Nucleic Acids Res. 2012 Nov 1;40(20):10254-62
references: 22965122
references: Plant Sci. 2011 Apr;180(4):620-7
references: 21421410
references: Infect Immun. 1996 Nov;64(11):4826-9
references: 8890245
references: Annu Rev Microbiol. 2006;60:131-47
references: 16704339
references: Infect Immun. 2004 Jan;72(1):168-75
references: 14688094
references: Nature. 2000 Jun 29;405(6790):1073-7
references: 10890451
references: J Bacteriol. 2007 Aug;189(16):5916-28
references: 17586645
references: J Bacteriol. 2003 Dec;185(23):6747-55
references: 14617638
references: Structure. 2011 Sep 7;19(9):1294-306
references: 21893288
references: EMBO J. 2000 Jun 1;19(11):2452-64
references: 10835344
references: Infect Immun. 2005 Jan;73(1):18-29
references: 15618137
references: J Bacteriol. 1998 May;180(9):2442-9
references: 9573197
references: Vet Immunol Immunopathol. 2013 Mar 15;152(1-2):121-5
references: 23068271
references: Mol Microbiol. 1999 Jul;33(2):296-306
references: 10411746
references: Infect Immun. 1995 Nov;63(11):4323-8
references: 7591066
references: EcoSal Plus. 2005 Nov;1(2):
references: 26443515
references: Mol Microbiol. 2001 Feb;39(3):664-78
references: 11169107
references: J Biol Chem. 1996 Aug 23;271(34):20359-64
references: 8702771
references: FEMS Microbiol Lett. 2010 Jul;308(2):123-9
references: 20497228
references: J Clin Invest. 1993 Sep;92(3):1418-24
references: 8376595
references: J Bacteriol. 1998 Feb;180(3):722-31
references: 9457880
references: J Bacteriol. 2011 Apr;193(7):1622-32
references: 21278287
references: J Bacteriol. 2011 Dec;193(24):6843-51
references: 21984790
references: Infect Immun. 1997 Jul;65(7):2606-12
references: 9199427
references: Int J Med Microbiol. 2006 Aug;296(4-5):197-210
references: 16618552
references: Mol Microbiol. 1995 Nov;18(4):661-70
references: 8817489
references: Infect Immun. 2002 Jul;70(7):3500-9
references: 12065489
references: Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8
references: 7878036
references: Adv Exp Med Biol. 1997;412:247-8
references: 9192021
references: J Dairy Sci. 2012 Jun;95(6):3318-26
references: 22612965
references: Vet Microbiol. 2003 Oct 30;96(3):259-66
references: 14559173
references: Cell Microbiol. 2006 Sep;8(9):1488-503
references: 16922867
references: Mol Microbiol. 2004 Jan;51(1):73-87
references: 14651612
references: DNA Res. 2001 Feb 28;8(1):11-22
references: 11258796
references: J Clin Microbiol. 2004 Mar;42(3):1382-3
references: 15004128
references: Infect Immun. 2002 Aug;70(8):4362-8
references: 12117946
references: Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6754-9
references: 19366662
references: Infect Immun. 1998 Aug;66(8):3810-7
references: 9673266
references: Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17739-44
references: 20876094
references: Nature. 2001 Jan 25;409(6819):529-33
references: 11206551
references: J Clin Microbiol. 1999 Aug;37(8):2719-22
references: 10405431
references: Clin Vaccine Immunol. 2008 Aug;15(8):1208-13
references: 18562563
references: Infect Immun. 2000 Sep;68(9):5090-5
references: 10948130
references: Nat Rev Microbiol. 2004 May;2(5):391-400
references: 15100692
references: Appl Environ Microbiol. 2009 Jan;75(1):64-71
references: 18978078
references: J Clin Microbiol. 2002 Dec;40(12):4486-92
references: 12454140
references: BMC Genomics. 2009 Aug 03;10:349
references: 19650909
references: Infect Immun. 2001 Nov;69(11):6999-7009
references: 11598075
references: Infect Immun. 2002 May;70(5):2704-7
references: 11953416
references: Infect Immun. 1994 Aug;62(8):3494-505
references: 7518809
references: J Bacteriol. 2001 Aug;183(16):4727-36
references: 11466275
references: Int J Med Microbiol. 2008 Oct;298(7-8):571-8
references: 18272428
references: Biochem Biophys Res Commun. 2008 May 16;369(4):1061-4
references: 18342623
references: J Biol Chem. 2010 Oct 15;285(42):32336-42
references: 20688909
references: Infect Immun. 2001 Oct;69(10):6323-35
references: 11553577
references: Infect Immun. 1996 Jan;64(1):146-53
references: 8557332
references: Infect Immun. 2010 Aug;78(8):3625-36
references: 20547747
references: Infect Immun. 2005 Mar;73(3):1836-46
references: 15731085
references: J Bacteriol. 2009 Jan;191(1):411-21
references: 18952791
references: Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43
references: 2172966
references: Infect Immun. 1999 May;67(5):2389-98
references: 10225900
references: Mol Microbiol. 1992 Feb;6(3):411-7
references: 1552854
references: Mol Microbiol. 2002 Apr;44(2):361-79
references: 11972776
references: Mol Microbiol. 1999 Dec;34(5):941-52
references: 10594820
references: Microbiology (Reading). 2004 Nov;150(Pt 11):3631-3645
references: 15528651
references: Mol Microbiol. 1999 Apr;32(1):151-8
references: 10216868
references: Microbiology (Reading). 2007 Apr;153(Pt 4):959-967
references: 17379706
references: Infect Immun. 1996 Jun;64(6):2225-33
references: 8675331
references: Infect Immun. 2002 Jun;70(6):3085-93
references: 12011002
references: Infect Immun. 2010 Oct;78(10):4294-301
references: 20643852
references: J Clin Microbiol. 2009 Aug;47(8):2442-51
references: 19494071
references: J Bacteriol. 2007 Jul;189(14):5387-92
references: 17496094
references: Microbiol Mol Biol Rev. 1998 Jun;62(2):379-433
references: 9618447
references: Mol Microbiol. 1998 Apr;28(1):1-4
references: 9593291
references: Infect Immun. 2007 May;75(5):2432-40
references: 17339361
references: FEMS Immunol Med Microbiol. 2012 Dec;66(3):399-410
references: 23163871
references: Infect Immun. 1995 Sep;63(9):3316-26
references: 7543880
references: J Bacteriol. 2009 Jun;191(11):3451-61
references: 19218393
references: Pediatr Nephrol. 2002 Mar;17(3):201-11
references: 11956862
references: J Clin Microbiol. 1999 Oct;37(10):3357-61
references: 10488206
references: J Infect Dis. 2000 Apr;181(4):1496-500
references: 10762584
references: Microbiology (Reading). 2009 Nov;155(Pt 11):3554-3563
references: 19684070
references: J Clin Microbiol. 2007 Mar;45(3):1038-41
references: 17229860
references: Cell Microbiol. 2004 Dec;6(12):1167-83
references: 15527496
references: Environ Microbiol. 2009 Jul;11(7):1815-26
references: 19508558
references: Environ Microbiol. 2008 Mar;10(3):589-604
references: 18237301
references: Nature. 1989 Apr 20;338(6217):652-5
references: 2649795
references: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt 8):929-31
references: 20693671
references: Infect Immun. 1994 May;62(5):1835-42
references: 8168946
references: Infect Immun. 2002 May;70(5):2414-8
references: 11953378
references: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3597-602
references: 14988506
references: Mol Microbiol. 2002 Aug;45(4):951-66
references: 12180916
references: Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7991-5
references: 7644526
references: Mol Cell Probes. 1997 Dec;11(6):397-406
references: 9500807
references: Int J Med Microbiol. 2007 Jun;297(3):177-85
references: 17353147
references: Curr Top Microbiol Immunol. 2002;264(1):31-48
references: 12014180
references: Infect Immun. 2000 Jan;68(1):64-71
references: 10603369
references: Infect Immun. 2005 Oct;73(10):6608-19
references: 16177337
references: Mol Microbiol. 1997 May;24(4):767-78
references: 9194704
references: J Bacteriol. 2007 Oct;189(20):7426-35
references: 17693516
references: Mol Microbiol. 1999 Feb;31(4):1229-41
references: 10096089
references: Res Microbiol. 2006 Apr;157(3):263-6
references: 16171978
references: J Appl Microbiol. 2005;99(2):418-25
references: 16033475
references: Infect Immun. 2003 Sep;71(9):4985-95
references: 12933841
references: FEMS Microbiol Lett. 2007 Jun;271(2):258-64
references: 17451446
references: Infect Immun. 1995 Sep;63(9):3739-44
references: 7642319
references: FEMS Microbiol Lett. 1999 Sep 1;178(1):177-82
references: 10483737
references: Infect Immun. 2001 Apr;69(4):2107-15
references: 11254564
references: Mol Microbiol. 1997 Apr;24(1):7-17
references: 9140961
references: Appl Environ Microbiol. 2010 Jul;76(13):4461-8
references: 20453145
references: Infect Immun. 1998 Mar;66(3):944-9
references: 9488380
references: Environ Microbiol. 2011 Jan;13(1):62-73
references: 20649646
references: Environ Microbiol. 2006 Jun;8(6):1033-47
references: 16689724
references: Infect Immun. 2001 Sep;69(9):5597-605
references: 11500434
references: Infect Immun. 2001 Mar;69(3):1231-43
references: 11179284
references: Infect Immun. 2002 Aug;70(8):4556-63
references: 12117968
references: Infect Immun. 2005 May;73(5):2573-85
references: 15845459
references: Infect Immun. 2006 Oct;74(10):5747-55
references: 16988252
references: Environ Microbiol. 2009 Jul;11(7):1803-14
references: 19508554
references: Vet Microbiol. 1998 Mar 31;61(3):191-7
references: 9631531
references: FEMS Microbiol Lett. 2003 Jan 28;218(2):311-6
references: 12586409
references: J Clin Invest. 2007 Nov;117(11):3519-29
references: 17948128
references: Infect Immun. 2012 Mar;80(3):914-20
references: 22232190
references: Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10637-42
references: 17563352
references: Appl Environ Microbiol. 2005 Jan;71(1):247-54
references: 15640194
references: Environ Microbiol. 2009 Apr;11(4):992-1006
references: 19187284
references: Infect Immun. 2008 Nov;76(11):5072-81
references: 18794292
references: FEMS Microbiol Lett. 2004 Sep 15;238(2):333-44
references: 15358418
references: Vet Immunol Immunopathol. 2013 Mar 15;152(1-2):146-55
references: 23084628
references: Biochem Biophys Res Commun. 2005 May 20;330(4):1199-204
references: 15823570
references: Infect Immun. 1999 Apr;67(4):2045-9
references: 10085058
references: J Bacteriol. 1995 Apr;177(8):2087-97
references: 7721701
references: Cell Microbiol. 2009 Jan;11(1):121-37
references: 19016776
references: Infect Immun. 2008 Nov;76(11):5062-71
references: 18794291
references: Infect Immun. 2002 Dec;70(12):6761-9
references: 12438351
references: Infect Immun. 1996 Nov;64(11):4472-9
references: 8890194
references: Infect Immun. 1995 Sep;63(9):3621-7
references: 7642299
references: Curr Opin Microbiol. 2003 Feb;6(1):82-90
references: 12615225
references: Appl Environ Microbiol. 2009 Sep;75(18):5779-86
references: 19633120
references: Infect Immun. 2001 Jul;69(7):4447-57
references: 11401985
references: Infect Immun. 2011 Sep;79(9):3744-50
references: 21708988
references: Mol Microbiol. 2002 Jun;44(6):1533-50
references: 12067342
references: Infect Immun. 2000 Mar;68(3):1400-7
references: 10678953
references: J Med Microbiol. 2000 Jan;49(1):97-101
references: 10628831
references: Infect Immun. 1998 Sep;66(9):4560-3
references: 9712821
references: EMBO J. 1996 Jun 3;15(11):2613-24
references: 8654358
references: Vet Immunol Immunopathol. 2007 Aug 15;118(3-4):229-38
references: 17617472
references: Infect Immun. 2006 Feb;74(2):1255-65
references: 16428775
references: Appl Environ Microbiol. 2005 Dec;71(12):8008-15
references: 16332780
references: Microbiology (Reading). 2001 Jan;147(Pt 1):145-52
references: 11160808
references: FEMS Microbiol Lett. 1999 Oct 15;179(2):467-72
references: 10518752
references: Mol Microbiol. 1993 Feb;7(4):523-36
references: 8459772
references: Microbiology (Reading). 2010 May;156(Pt 5):1303-1312
references: 20110300
references: Infect Immun. 2006 May;74(5):2676-85
references: 16622204
references: FEMS Microbiol Lett. 1999 Sep 1;178(1):169-75
references: 10483736
references: Nat Struct Biol. 1999 Apr;6(4):313-8
references: 10201396
references: J Clin Invest. 1993 Sep;92(3):1412-7
references: 8376594
references: J Biol Chem. 2002 Jan 25;277(4):2876-85
references: 11704679
references: J Biol Chem. 2004 Aug 6;279(32):33751-8
references: 15173179