title: Coiled-coils in phage display screening: insight into exceptional selectivity provided by molecular dynamics.
abstract: Involved in numerous key biological functions, protein helix-helix interactions follow a well-defined intermolecular recognition pattern. The characteristic structure of the α-helical coiled-coil allows for the specific randomization of clearly defined interaction partners within heteromeric systems. In this work, a rationally designed heterodimeric coiled-coil was used to investigate potential factors influencing the sequence selectivity in interhelical interactions.
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Jérémie
authors: J
authors: Mortier
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Elisabeth K
authors: EK
authors: Nyakatura
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Oliver
authors: O
authors: Reimann
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Susanne
authors: S
authors: Huhmann
authors: ‡Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
authors: Jan O
authors: JO
authors: Daldrop
authors: ||Fritz Haber Institute der MPG, Faradayweg 4-6, 14195 Berlin, Germany.
authors: Carsten
authors: C
authors: Baldauf
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Gerhard
authors: G
authors: Wolber
authors: ‡Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
authors: Markus S
authors: MS
authors: Miettinen
authors: †Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors: Beate
authors: B
authors: Koksch
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D002942:Circular Dichroism; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D019151:Peptide Library; D011487:Protein Conformation; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry
chemical_list: D019151:Peptide Library; D011506:Proteins; D014443:Tyrosine