title: Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma.
abstract: The purpose of this study was to determine the effect of aerobic exercise training (AT) on the expression of glucocorticoid receptors (GR) and anti-inflammatory cytokines in an asthma model. BALB/c mice were divided into groups control (CT; nonsensitized/nontrained), aerobic training (AT; nonsensitized/trained), ovalbumin (OVA; sensitized/not trained), and OVA+AT (sensitized/trained). OVA groups received OVA by inhalation, and the AT groups completed 1, 3, or 7 days of exercise (60 min/session). Expression of GR, IL-4, IL-5, IL-10, IL-1ra, NF-κB, TGF-β, VEGF, ICAM-1, VCAM-1; eosinophils counting; and airway remodeling (AR) features [airway smooth muscle (ASM) and epithelial thickness and collagen fiber deposition] were quantified. OVA sensitization induced a decrease in the expression of GR and increases in the eosinophil, IL-4, IL-5, NF-κB, TGF-β, VEGF, ICAM-1, VCAM-1, and AR features (P < 0.05). After 3 days, AT reversed the OVA-induced reduction in the expression of GR, and subsequently induced increases in the expression of IL-10 and IL-1ra (seventh day). In contrast, the eosinophil migration, the expression of NF-κB, IL-4, IL-5, TGF-β, RANTES, VEGF, ICAM-1, VCAM-1, and the AR features (P < 0.05) were reduced. AT increases the expression of GR and anti-inflammatory cytokines (IL-10 and IL-1ra) and reduces the expression of inflammatory mediators and airway inflammation in an animal model of asthma.
authors: Department of Physical Therapy, School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.|Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.
authors: R A
authors: RA
authors: Silva
authors: Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.
authors: F M
authors: FM
authors: Almeida
authors: Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.
authors: C R
authors: CR
authors: Olivo
authors: Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.|University City of São Paulo (UNICID), São Paulo, SP, Brazil.
authors: B M
authors: BM
authors: Saraiva-Romanholo
authors: Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.
authors: M A
authors: MA
authors: Martins
authors: Department of Physical Therapy, School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.|Department of Clinical Medicine (LIM-20), School of Medicine, University of Sao Paulo, São Paulo, SP, Brazil.
authors: C R F
authors: CR
authors: Carvalho
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D001938:Brazil; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D007958:Leukocyte Count; D008168:Lung / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D010805:Physical Conditioning, Animal; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000276:immunology*
chemical_list: D016207:Cytokines; D011965:Receptors, Glucocorticoid; D010047:Ovalbumin
keywords: Exercise; allergy; cytokine regulator; hormone; structural changes