title: Discriminating patients with early-stage pancreatic cancer or chronic pancreatitis using serum electrospray mass profiling.
abstract: Blood tests are needed to aid in the early detection of pancreatic ductal adenocarcinoma (PDAC), and monitoring pancreatitis development into malignancy especially in high risk patients. This study exhibits efforts and progress toward developing such blood tests, using electrospray-mass spectrometry (MS) serum profiling to distinguish patients with early-stage PDAC or pancreatitis from each other and from controls. Identification of significant serum mass peak differences between these individuals was performed using t tests and "leave one out" cross validation. Serum mass peak distributions of control individuals were distinguished from those of patients with chronic pancreatitis or early-stage PDAC with P values <10(-15), and patients with chronic pancreatitis were distinguished from those of patients with early-stage PDAC with a P value <10(-12). Sera from 12 out of 12 patients with PDAC stages I, IIA and IIB were blindly validated from controls. Tandem MS/MS identified a cancer phenotype with elements of PDAC involved in early-stage PDAC/control discrimination. These studies indicate electrospray-MS mass profiling can detect serum changes in patients with pancreatitis or early-stage pancreatic cancer. Such technology has the potential to aid in early detection of pancreatic cancer, biomarker development, and in monitoring development of pancreatitis into PDAC.
authors: Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: James R
authors: JR
authors: Hocker
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Russell G
authors: RG
authors: Postier
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Min
authors: M
authors: Li
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States; Veterans Affairs Medical Center, Oklahoma City, OK, United States.
authors: Megan R
authors: MR
authors: Lerner
authors: Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Stan A
authors: SA
authors: Lightfoot
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Marvin D
authors: MD
authors: Peyton
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Subrato J
authors: SJ
authors: Deb
authors: Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Candace M
authors: CM
authors: Baker
authors: Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Travis L
authors: TL
authors: Williams
authors: Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Rushie Jane
authors: RJ
authors: Hanas
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Donald E
authors: DE
authors: Stowell
authors: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Theresa J
authors: TJ
authors: Lander
authors: Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
authors: Daniel J
authors: DJ
authors: Brackett
authors: Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States; Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States; Veterans Affairs Medical Center, Oklahoma City, OK, United States. Electronic address: jay-hanas@ouhsc.edu.
authors: Jay S
authors: JS
authors: Hanas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000175:diagnosis*; D003937:Diagnosis, Differential; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D050500:Pancreatitis, Chronic / Q000097:blood / Q000175:diagnosis*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor
keywords: Cancer phenotype; Chronic pancreatitis; Early detection; Electrospray mass spectrometry; Pancreatic ductal adenocarcinoma; Serum mass profiling
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