title: Determination of steroid hormones in human plasma by GC-triple quadrupole MS.
abstract: A fast and sensitive GC-MS/MS method is proposed to determine pregnenolone, dehydroepiandrosterone (DHEA), testosterone and dihydrotestosterone from human plasma. Steroids were extracted by liquid/liquid extraction, and derivatized with N-methyl-N-trimethylsilyl-trifluoracetamide. Electron ionization at 30eV was applied to generate high abundant precursor ions. Specific precursor/product transitions were generated a priori for each compound of interest and these assays were then used to selectively detect and quantify these steroids. Using the selected reaction monitoring mode, detection limits in the pg/ml (subnmol/l) range could be achieved for all four steroid compounds. The method was validated for imprecision, and recovery and applied in a pilot study in patients with hypercholesterolemia. Associations of increased cholesterol biosynthesis and low testosterone and dihydrotestosterone concentrations were found.
authors: University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. Electronic address: silke.matysik@klinik.uni-regensburg.de.
authors: Silke
authors: S
authors: Matysik
authors: University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
authors: Gerd
authors: G
authors: Schmitz
mesh_terms: D000328:Adult; D002940:Circadian Rhythm; D003687:Dehydroepiandrosterone / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006728:Hormones / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D013256:Steroids / Q000097:blood*; D013261:Sterols / Q000097:blood; D013997:Time Factors
chemical_list: D006728:Hormones; D013256:Steroids; D013261:Sterols; D003687:Dehydroepiandrosterone
keywords: DHEA; Dihydrotestosterone; Pregnenolone; Steroid hormones; Testosterone