title: Effect of clathrin light chains on the stiffness of clathrin lattices and membrane budding.
abstract: Clathrin-dependent transport processes require the polymerization of clathrin triskelia into polygonal scaffolds. Together with adapter proteins, clathrin collects cargo and induces membrane bud formation. It is not known to what extent clathrin light chains affect the structural and functional properties of clathrin lattices and the ability of clathrin to deform membranes. To address these issues, we have developed a novel procedure for analyzing clathrin lattice formation on rigid surfaces. We found that lattices can form on adaptor-coated convex-, planar- and even shallow concave surfaces, but the rate of formation and resistance to thermal dissociation of the lattice are greatly enhanced on convex surfaces. Atomic force microscopy on planar clathrin lattices demonstrates that the stiffness of the clathrin lattice is strictly dependent on light chains. The reduced stiffness of the lattice also compromised the ability of clathrin to generate coated buds on the surface of rigid liposomal membranes.
authors: Institute of Cell Biology, Centre of Anatomy, Hannover Medical School, Carl-Neuberg Street 1, D-30625, Hannover, Germany.
authors: Philip N
authors: PN
authors: Dannhauser
authors: Mitja
authors: M
authors: Platen
authors: Heike
authors: H
authors: Böning
authors: Huberta
authors: H
authors: Ungewickell
authors: Iwan A T
authors: IA
authors: Schaap
authors: Ernst J
authors: EJ
authors: Ungewickell
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D001665:Binding Sites; D033941:Clathrin Light Chains / Q000378:metabolism / Q000648:ultrastructure*; D022163:Clathrin-Coated Vesicles / Q000378:metabolism / Q000648:ultrastructure*; D008081:Liposomes / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D011145:Polyvinyls / Q000737:chemistry; D013499:Surface Properties
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D033941:Clathrin Light Chains; D008081:Liposomes; D011145:Polyvinyls; C114154:epsin; C013215:polyvinyl acetate
keywords: Hsc70; atomic force microscopy; curvature; electron microscopy; epsin; planar lattices; uncoating