title: Effects of short- and long-term Mediterranean-based dietary treatment on plasma LC-QTOF/MS metabolic profiling of subjects with metabolic syndrome features: The Metabolic Syndrome Reduction in Navarra (RESMENA) randomized controlled trial.
abstract: METHODS
  Adherence to the Mediterranean diet has been associated with a reduced risk of metabolic syndrome (MetS). Metabolomics approach may contribute to identify beneficial associations of metabolic changes affected by Mediterranean diet-based interventions with inflammatory and oxidative-stress markers related to the etiology and development of the MetS.
  
  
  RESULTS
  Liquid chromatography coupled to quadrupole-time of flight-MS metabolic profiling was applied to plasma from a 6-month randomized intervention with two sequential periods, a 2-month nutritional-learning intervention period, and a 4-month self-control period, with two energy-restricted diets; the RESMENA diet (based on the Mediterranean dietary pattern) and the Control diet (based on the American Heart Association guidelines), in 72 subjects with a high BMI and at least two features of MetS. The major contributing biomarkers of each sequential period were lipids, mainly phospholipids and lysophospholipids. Dependency network analysis showed a different pattern of associations between metabolic changes and clinical variables after 2 and 6 month of intervention, with a highly interconnected network during the nutritional-learning intervention period of the study.
  
  
  CONCLUSIONS
  The 2-month RESMENA diet produced significant changes in the plasma metabolic profile of subjects with MetS features. However, at the end of the 6-month study, most of the associations between metabolic and clinical variables disappeared; suggesting that adherence to healthy dietary habits had declined during the self-control period.
authors: VTT Technical Research Centre of Finland, Espoo, Finland; Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.
authors: Isabel
authors: I
authors: Bondia-Pons
authors: José Alfredo
authors: JA
authors: Martinez
authors: Rocio
authors: R
authors: de la Iglesia
authors: Patricia
authors: P
authors: Lopez-Legarrea
authors: Kaisa
authors: K
authors: Poutanen
authors: Kati
authors: K
authors: Hanhineva
authors: Maria de los Ángeles
authors: Mde L
authors: Zulet
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D001835:Body Weight; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D038441:Diet, Mediterranean; D006801:Humans; D013058:Mass Spectrometry; D024821:Metabolic Syndrome / Q000097:blood* / Q000178:diet therapy*; D055442:Metabolome; D055432:Metabolomics; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D044465:White People
chemical_list: D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides
keywords: Dependency networks; LC-QTOF/MS; Mediterranean diet; Metabolic syndrome; Metabolomics