title: Paper-based analytical devices for electrochemical study of the breathing process of red blood cells.
abstract: Herein we utilized the filter paper to physically trap red blood cells (RBC) to observe the breathing process of red blood cells based on the permeability of the filter paper. By integrating double-sided conductive carbon tape as the working electrodes, the device could be applied to monitor electrochemical responses of RBC for up to hundreds of minutes. The differential pulse voltammetry (DPV) peak currents increased under oxygen while decreased under nitrogen, indicating that RBC could take in and release oxygen. Further studies demonstrated that the RBC suspension could more effectively take in oxygen than the solution of hemoglobin and the supernatant of RBC, suggesting the natural advantage of RBC on oxygen transportation. This study implied that simple paper-based analytical devices might be effectively applied in the study of gas-participating reactions and biochemical detections.
authors: School of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China; Institute of Nautical Medicine, Nantong University, 9 Seyuan Road, Nantong 226019, China.
authors: Xiang-Yun
authors: XY
authors: Lin
authors: School of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China.
authors: Ling-Ling
authors: LL
authors: Wu
authors: School of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China.
authors: Zhong-Qin
authors: ZQ
authors: Pan
authors: School of Chemistry and Chemical Engineering, Nantong University, 9 Seyuan Road, Nantong 226019, China.
authors: Chuan-Guo
authors: CG
authors: Shi
authors: School of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China. Electronic address: ningbao@ntu.edu.cn.
authors: Ning
authors: N
authors: Bao
authors: School of Public Health, Nantong University, 9 Seyuan Road, Nantong 226019, China; Institute of Nautical Medicine, Nantong University, 9 Seyuan Road, Nantong 226019, China. Electronic address: hygu@ntu.edu.cn.
authors: Hai-Ying
authors: HY
authors: Gu
mesh_terms: D019069:Cell Respiration; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D010209:Paper; D010539:Permeability
chemical_list: D010108:Oxyhemoglobins; D009584:Nitrogen; D010100:Oxygen
keywords: Breathing process; Cell trapping; Oxyhemoglobin; Paper-based analytical device; Permeability