title: Neural stem cell progeny regulate stem cell death in a Notch and Hox dependent manner.
abstract: Cell death is a prevalent, well-controlled and fundamental aspect of development, particularly in the nervous system. In Drosophila, specific neural stem cells are eliminated by apoptosis during embryogenesis. In the absence of apoptosis, these stem cells continue to divide, resulting in a dramatically hyperplastic central nervous system and adult lethality. Although core cell death pathways have been well described, the spatial, temporal and cell identity cues that activate the cell death machinery in specific cells are largely unknown. We identified a cis-regulatory region that controls the transcription of the cell death activators reaper, grim and sickle exclusively in neural stem cells. Using a reporter generated from this regulatory region, we found that Notch activity is required for neural stem cell death. Notch regulates the expression of the abdominalA homeobox protein, which provides important spatial cues for death. Importantly, we show that pro-apoptotic Notch signaling is activated by the Delta ligand expressed on the neighboring progeny of the stem cell. Thus we identify a previously undescribed role for progeny in regulating the proper developmental death of their parental stem cells.
authors: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors: R
authors: R
authors: Arya
authors: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors: T
authors: T
authors: Sarkissian
authors: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors: Y
authors: Y
authors: Tan
authors: CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
authors: K
authors: K
authors: White
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D016923:Cell Death / Q000235:genetics / Q000502:physiology; D004330:Drosophila; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051880:Receptors, Notch; D014157:Transcription Factors
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