title: Preparation and regulating cell adhesion of anion-exchangeable layered double hydroxide micropatterned arrays.
abstract: We describe a reliable preparation of MgAl-layered double hydroxide (MgAl-LDH) micropatterned arrays on gold substrate by combining SO3(-)-terminated self-assembly monolayer and photolithography. The synthesis route is readily extended to prepare LDH arrays on the SO3(-)-terminated polymer-bonded glass substrate amenable for cell imaging. The anion-exchangeable MgAl-LDH micropattern can act both as bioadhesive region for selective cell adhesion and as nanocarrier for drug molecules to regulate cell behaviors. Quantitative analysis of cell adhesion shows that selective HepG2 cell adhesion and spreading are promoted by the micropatterned MgAl-LDH, and also suppressed by methotrexate drug released from the LDH interlayer galleries.
authors: State Key Laboratory of Chemical Resource Engineering, §Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, and ⊥Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
authors: Feng
authors: F
authors: Yao
authors: Hao
authors: H
authors: Hu
authors: Sailong
authors: S
authors: Xu
authors: Ruijie
authors: R
authors: Huo
authors: Zhiping
authors: Z
authors: Zhao
authors: Fazhi
authors: F
authors: Zhang
authors: Fujian
authors: F
authors: Xu
mesh_terms: D000838:Anions / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects*; D002477:Cells / Q000166:cytology / Q000187:drug effects; D016503:Drug Delivery Systems / Q000295:instrumentation*; D056945:Hep G2 Cells; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D008727:Methotrexate / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000838:Anions; D006878:Hydroxides; C031356:hydroxide ion; D008727:Methotrexate
keywords: cell adhesion; in situ growth; layered double hydroxide; methotrexate; micropatterned arrays; photolithography