title: Short communication: Difructose anhydride III promotes calcium absorption from the duodenum in cattle.
abstract: Difructose anhydride (DFA) III promotes the intestinal absorption of calcium via a paracellular pathway in rats. In dairy cows, DFA III reaches the duodenum without being degraded by ruminal bacteria and hence could be used to control hypocalcemia. The aims of the present study were to investigate the percentage of DFA III that appears in the duodenum of cows and to determine the effect of DFA III on calcium absorption from duodenal fluid. The first experiment was performed in 3 ruminally and duodenally cannulated dry Holstein cows in a 3 × 3 Latin square design. Each experimental period lasted 7 d. On the first day, the cows were ruminally fed one of the following treatments: 0 (DFA0), 50 (DFA50), or 100 (DFA100) g/d of DFA III, using cobalt-EDTA as a liquid phase marker. Difructose anhydride III was detected in duodenal fluid 1 h after feeding, and its concentration peaked 4 h after feeding, in a dose-dependent manner. The percentages of DFA III that appeared in the duodenum after the DFA50 and DFA100 treatments were 69.1 ± 7.0% and 67.9 ± 5.6%, respectively. The second experiment used the everted duodenal sacs of cattle (n = 7 in each group). Sacs were incubated in artificial mucosal fluid containing 1 mM DFA III or no DFA III (control) for 60 min with 100% O2 in a water bath at 37 °C. After incubation, the calcium concentration of the artificial serosal fluid in the everted sacs was measured. Calcium absorption was higher in the DFA III-treated group than in the control group (803 ± 161 and 456 ± 74 nmol/cm of sac, respectively). The above results demonstrate that approximately 70% of administered DFA III reached the duodenum of cows intact. Moreover, similar to its effects on calcium absorption in rats, DFA III promoted calcium absorption via a paracellular pathway in the duodenum of cows.
authors: United Graduate School of Agricultural Science, Iwate University, Morioka, Iwate, 020-8550, Japan; Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan. Electronic address: teramu@nitten.co.jp.
authors: M
authors: M
authors: Teramura
authors: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors: T
authors: T
authors: Nakai
authors: Hokkaido Research Organization, Agriculture Research Department Animal Research Center, Shintoku, Hokkaido, 081-0031, Japan.
authors: M
authors: M
authors: Itoh
authors: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors: T
authors: T
authors: Sato
authors: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors: M
authors: M
authors: Ohtani
authors: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors: C
authors: C
authors: Kawashima
authors: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors: M
authors: M
authors: Hanada
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002136:Calcium, Dietary / Q000378:metabolism*; D002417:Cattle; D004187:Disaccharides / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D005260:Female; D005766:Gastrointestinal Contents / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects*; D051381:Rats
chemical_list: D002136:Calcium, Dietary; D004187:Disaccharides; C087758:difructose anhydride III; D002118:Calcium
keywords: calcium; difructose anhydride (DFA) III; duodenum