title: Unrevealing the role of P-protein on melanosome biology and structure, using siRNA-mediated down regulation of OCA2.
abstract: The pink-eyed dilution protein (P-protein) plays a critical role in melanin synthesis in melanocytes and retinal pigment epithelium cells. Mutation in this protein may cause complete or partial albinism. Role of the P-protein ranges in melanin synthesis to maturation and trafficking of the melanosomes. The aim of the present study was to evaluate the effect of P-protein inhibition on melanosome biology by comparing the shape, size, count, and types of melanosomes in melan-a melanocytes. The cells were extensively examined by the transmission electron microscopy. The P-protein inhibition was carried by P-protein-siRNA transfection to melan-a melanocytes, B16F10 mouse melanoma, and melan-p1 cells. Measurement of melanin contents, cellular tyrosinase, and different tyrosinase related proteins were also determined to investigate the effect of P-protein siRNA transfection on melanocytes. Results suggested that the inhibition of P-protein can significantly change the melanosomal morphology, types and their respective numbers, and provided a novel strategy for the control of melanin synthesis.
authors: Department of Biological Engineering, Inha University, Nam-gu, Incheon, 402751, Republic of Korea.
authors: Sangjoo
authors: S
authors: Park
authors: V K
authors: VK
authors: Morya
authors: Dong Hoang
authors: DH
authors: Nguyen
authors: Birendra K
authors: BK
authors: Singh
authors: Hyang-Bok
authors: HB
authors: Lee
authors: Eun-Ki
authors: EK
authors: Kim
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D020460:Melanosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection; D014443:Tyrosine / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D008543:Melanins; D008565:Membrane Proteins; D034741:RNA, Small Interfering; C076239:P protein, mouse; D014443:Tyrosine; D014442:Monophenol Monooxygenase
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