title: Clinical significance of aberrant Wnt7a promoter methylation in human non-small cell lung cancer in Koreans.
abstract: The Wnt signaling pathway has regulatory roles in cell proliferation, differentiation, and polarity. Aberrant Wnt pathway regulation can lead to abnormal cell proliferation and cancer, and loss of Wnt7a expression has been demonstrated in lung cancer cell lines. E-cadherin keeps intercellular integrity and prevents metastasis. Therefore, E-cadherin has been known as a prognostic factor in cancer. In the present study, we investigated the E-cadherin expression status by immunohistochemical stain and the Wnt7a promoter methylation status in human non-small cell lung carcinoma (NSCLC) by methylation-specific PCR. We also analyzed their correlations with clinicopathological factors. Methylation of the Wnt7a gene promoter was detected in the lung tissues of 32 of 121 (26.4%) patients with NSCLC. Wnt7a promoter methylation was correlated with advanced tumor stage (P = 0.036) and distant metastasis (P = 0.037). In addition, Wnt7a promoter methylation showed correlation with loss of E-cadherin expression (P < 0.001). However, Wnt7a promoter methylation was not closely related with gender, age, histological type, or smoking habit. Even though Wnt7a methylation could not show significant correlation with the long term survival of the patients with limited follow up data, these findings suggest that loss of the Wnt7a gene induced by promoter methylation might be another prognostic factor for NSCLC and that restoration of Wnt7a may be a promising treatment for NSCLC.
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Tae-Hyung
authors: 0000-0001-7132-2988
authors: TH
authors: Kim
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Ji-Yong
authors: 0000-0003-2459-3448
authors: JY
authors: Moon
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Sang-Heon
authors: 0000-0001-8398-4444
authors: SH
authors: Kim
authors: Department of Pathology, Hanyang University College of Medicine, Seoul, Korea.
authors: Seung Sam
authors: 0000-0002-8607-4072
authors: SS
authors: Paik
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Ho Joo
authors: 0000-0002-4645-4863
authors: HJ
authors: Yoon
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Dong Ho
authors: 0000-0002-5911-3216
authors: DH
authors: Shin
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Sung Soo
authors: 0000-0003-4736-1447
authors: SS
authors: Park
authors: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors: Jang Won
authors: 0000-0002-3863-7854
authors: JW
authors: Sohn
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic / Q000235:genetics*; D056910:Republic of Korea; D051153:Wnt Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C092778:WNT7A protein, human; D051153:Wnt Proteins
keywords: Biologic Markers; Carcinoma, Non-small Cell Lung; E-cadherin; Wnt7a
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