title: Constitutive expression of McCHIT1-PAT enhances resistance to rice blast and herbicide, but does not affect grain yield in transgenic glutinous rice.
abstract: To produce new rice blast- and herbicide-resistant transgenic rice lines, the McCHIT1 gene encoding the class I chitinase from Momordica charantia and the herbicide resistance gene PAT were introduced into Lailong (Oryza sativa L. ssp. Japonica), a glutinous local rice variety from Guizhou Province, People's Republic of China. Transgenic lines were identified by ß-glucuronidase (GUS) histochemical staining, PCR, and Southern blot analyses. Agronomic traits, resistance to rice blast and herbicide, chitinase activities, and transcript levels of McCHIT1 were assessed in the T2 progeny of three transgenic lines (L1, L8, and L10). The results showed that the introduction of McCHIT1-PAT into Lailong significantly enhanced herbicide and blast resistance. After infection with the blast fungus Magnaporthe oryzae, all of the T2 progeny exhibited less severe lesion symptoms than those of wild type. The disease indices were 100% for wild type, 65.66% for T2 transgenic line L1, 59.69% for T2 transgenic line L8, and 79.80% for T2 transgenic line L10. Transgenic lines expressing McCHIT1-PAT did not show a significant difference from wild type in terms of malondialdehyde (MDA) content, polyphenol oxidase (PPO) activity, and superoxide dismutase (SOD) activity in the leaves. However, after inoculation with M. oryzae, transgenic plants showed significantly higher SOD and PPO activities and lower MDA contents in leaves, compared with those in wild-type leaves. The transgenic and the wild-type plants did not show significant differences in grain yield parameters including plant height, panicles per plant, seeds per panicle, and 1000-grain weight. Therefore, the transgenic plants showed increased herbicide and blast resistance, with no yield penalty.
authors: The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang, Guizhou, People's Republic of China.|The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, People's Republic of China.
authors: Xiao-Fang
authors: XF
authors: Zeng
authors: The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, People's Republic of China.
authors: Lei
authors: L
authors: Li
authors: The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, People's Republic of China.
authors: Jian-Rong
authors: JR
authors: Li
authors: The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang, Guizhou, People's Republic of China.|The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, People's Republic of China.
authors: De-Gang
authors: DG
authors: Zhao
mesh_terms: D002688:Chitinases / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006540:Herbicides / Q000378:metabolism; D020082:Magnaporthe / Q000502:physiology; D029777:Momordica charantia / Q000201:enzymology* / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D014170:Transformation, Genetic
chemical_list: D006540:Herbicides; D010940:Plant Proteins; D002688:Chitinases; C119825:chitinase C
keywords: Lailong; Magnaporthe oryzae; McCHIT1-PAT; herbicide resistance; rice blast