title: A photoperiod-regulating gene CONSTANS is correlated to lipid biosynthesis in Chlamydomonas reinhardtii.
abstract: Background. The regulation of lipid biosynthesis is essential in photosynthetic eukaryotic cells. Thus far, no regulatory genes have been reported in the lipid metabolism pathway. Plant CONSTANS (CO) gene regulates blooming by participating in photoperiod and biological clock. Apart from regulating photoperiod, the Chlamydomonas CO gene also regulates starch content. Results. In this study, the results showed that, under HSM-S condition, cells accumulated more lipids at short-day conditions than at long-day conditions. The silencing of the CrCO gene via RNA interference resulted in an increase in lipid content and an increase in triacylglyceride (TAG) level by 24.5%. CrCO RNAi strains accumulated more lipids at short-day conditions than at long-day conditions. The decrease in CrCO expression resulted in the increased expression of TAG biosynthesis-related genes, such as DGAT2, PAP2, and PDAT3, whereas CIS and FBP1 genes showed a decrease in their mRNA when the CrCO expression was suppressed. On the other hand, the overexpression of CrCO resulted in the decrease in lipid content and TAG level. Conclusions. The results of this study revealed a relationship between CrCO gene and lipid metabolism in Chlamydomonas, suggesting that increasing oil by suppressing CrCO expression in microalgae is feasible.
authors: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China.
authors: Xiaodong
authors: X
authors: Deng
authors: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China.
authors: Xinzhao
authors: X
authors: Fan
authors: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China.
authors: Ping
authors: P
authors: Li
authors: School of Science, Hainan Medical College, Haikou 571101, China.
authors: Xiaowen
authors: X
authors: Fei
mesh_terms: D018533:Biomass; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005453:Fluorescence; D020868:Gene Silencing; D017343:Genes, Plant; D008055:Lipids / Q000096:biosynthesis*; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008055:Lipids; D010940:Plant Proteins; D012333:RNA, Messenger; D014280:Triglycerides
references: Plant Mol Biol. 1998 Jul;37(5):763-72
references: 9678571
references: Plant Cell. 2000 Dec;12(12):2473-2484
references: 11148291
references: Plant J. 2008 Apr;54(2):205-19
references: 18182030
references: Plant Cell. 2007 Oct;19(10):3242-55
references: 17965270
references: Plant J. 2003 Oct;36(1):82-93
references: 12974813
references: Plant Cell. 2009 Nov;21(11):3416-20
references: 19920209
references: Science. 2005 Jul 8;309(5732):293-7
references: 16002617
references: Plant J. 2007 Aug;51(4):563-74
references: 17605755
references: Differentiation. 2001 Mar;67(3):63-71
references: 11428128
references: Bioresour Technol. 2013 Oct;146:310-316
references: 23948268
references: Science. 1989 Dec 15;246(4936):1503-4
references: 17756009
references: Mol Cells. 1998 Aug 31;8(4):452-8
references: 9749533
references: Plant Cell. 2006 May;18(5):1152-65
references: 16617098
references: Plant Physiol Biochem. 2009 Feb;47(2):105-15
references: 19097801
references: Plant Cell. 2008 Sep;20(9):2324-38
references: 18796637
references: J Microbiol Methods. 2009 Apr;77(1):41-7
references: 19162091
references: J Microbiol Methods. 2008 Dec;75(3):437-40
references: 18706459
references: Plant Physiol. 2001 Apr;125(4):1821-30
references: 11299362
references: Biochem Cell Biol. 1998;76(2-3):351-8
references: 9923704
references: Plant Mol Biol. 2004 Sep;56(2):159-69
references: 15604735
references: Proc Natl Acad Sci U S A. 1990 Feb;87(3):1228-32
references: 2105499
references: Biosci Biotechnol Biochem. 2008 Jun;72(6):1539-49
references: 18540109
references: Plant J. 2006 May;46(3):462-76
references: 16623906
references: Plant Cell Physiol. 2007 Oct;48(10):1496-503
references: 17711875
references: Plant Physiol. 2006 Sep;142(1):305-17
references: 16844835
references: Plant Physiol. 2003 Apr;131(4):1855-67
references: 12692345
references: Gene. 2011 Jan 15;471(1-2):37-44
references: 20950678
references: Science. 2000 Aug 4;289(5480):768-71
references: 10926537
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Plant Physiol. 2005 Apr;137(4):1420-34
references: 15778459
references: Genes Dev. 2007 Oct 1;21(19):2371-84
references: 17908925
references: Curr Biol. 2009 Mar 10;19(5):359-68
references: 19230666
references: EMBO J. 2008 Apr 23;27(8):1277-88
references: 18388858
references: Plant J. 2001 Dec;28(6):619-31
references: 11851908
references: Mol Plant. 2012 Jul;5(4):945-7
references: 22511604
references: J Biotechnol. 2012 Nov 30;162(1):13-20
references: 22542934