title: Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats.
abstract: OBJECTIVE
  To investigate whether lycopene can modulate adiponectin levels and SIRT1 and FoxO1 gene expression in the adipose tissue of diet-induced obese rats.
  
  
  METHODS
  Male Wistar rats were first fed with hypercaloric diet (HD, n = 12) for 6 weeks, and afterward, these rats were randomly assigned to receive HD (n = 6) or HD with lycopene-rich tomato oleoresin (equivalent to lycopene 10 mg/kg body weight (BW)/day, HD + L, n = 6) by gavage for additional 6 weeks. Plasma lycopene and adiponectin levels were analyzed by high-performance liquid chromatography and immunoassay, respectively. The messenger RNA (mRNA) expressions of adiponectin, Sirtuin 1 (SIRT1), Forkhead box O 1 (FoxO1), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and PPARγ in adipose tissues were determined by quantitative polymerase chain reaction.
  
  
  RESULTS
  Lycopene was detected in the plasma of rats in HD + L group but not in the HD group. Although both BW and adiposity were not different between the two groups, there was a significant increase in both plasma concentration and mRNA expression of adiponectin in the adipose tissue of the HD + L group. In addition, the lycopene supplementation upregulated mRNA expressions of SIRT1, FoxO1, and FAT/CD36 but downregulated PPARγ in adipose tissue of obese rats.
  
  
  CONCLUSIONS
  These data suggest that lycopene, in the concentration used, is not toxic and also its health benefits in adipose tissue may play a role against obesity-related complications.
authors: Institute of Health Science, Federal University of Mato Grosso (UFMT), Sinop, Mato Grosso, Brazil Department of Internal Medicine, Botucatu Medical School (UNESP), Botucatu, São Paulo, Brazil reluvizotto@yahoo.com.
authors: R A M
authors: RA
authors: Luvizotto
authors: Institute of Health Science, Federal University of Mato Grosso (UFMT), Sinop, Mato Grosso, Brazil.
authors: A F
authors: AF
authors: Nascimento
authors: Department of Internal Medicine, Botucatu Medical School (UNESP), Botucatu, São Paulo, Brazil.
authors: N C M
authors: NC
authors: Miranda
authors: Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts, USA.
authors: X-D
authors: XD
authors: Wang
authors: Department of Internal Medicine, Botucatu Medical School (UNESP), Botucatu, São Paulo, Brazil.
authors: A L A
authors: AL
authors: Ferreira
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002338:Carotenoids / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000235:genetics; D000077276:Lycopene; D018551:Solanum lycopersicum; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D056564:Sirtuin 1 / Q000235:genetics; D000071161:Forkhead Box Protein O1
chemical_list: D052242:Adiponectin; D002338:Carotenoids; D051858:Forkhead Transcription Factors; D000077276:Lycopene; D009419:Nerve Tissue Proteins; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D056564:Sirtuin 1; C018620:oleoresins; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1; C488368:Sirt1 protein, rat
keywords: Adiponectin; FoxO1; SIRT1; lycopene; obesity