title: Cinnabar-induced subchronic renal injury is associated with increased apoptosis in rats.
abstract: The aim of this study was to explore the role of apoptosis in cinnabar-induced renal injury in rats. To test this role, rats were dosed orally with cinnabar (1 g/kg/day) for 8 weeks or 12 weeks, and the control rats were treated with 5% carboxymethylcellulose solution. Levels of urinary mercury (UHg), renal mercury (RHg), serum creatinine (SCr), and urine kidney injury molecule 1 (KIM-1) were assessed, and renal pathology was analyzed. Apoptotic cells were identified and the apoptotic index was calculated. A rat antibody array was used to analyze expression of cytokines associated with apoptosis. Results from these analyses showed that UHg, RHg, and urine KIM-1, but not SCr, levels were significantly increased in cinnabar-treated rats. Renal pathological changes in cinnabar-treated rats included vacuolization of tubular cells, formation of protein casts, infiltration of inflammatory cells, and increase in the number of apoptotic tubular cells. In comparison to the control group, expression of FasL, Fas, TNF-α, TRAIL, activin A, and adiponectin was upregulated in the cinnabar-treated group. Collectively, our results suggest that prolonged use of cinnabar results in kidney damage due to accumulation of mercury and that the underlying mechanism involves apoptosis of tubular cells via a death receptor-mediated pathway.
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Ying
authors: Y
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Dapeng
authors: D
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Jie
authors: J
authors: Wu
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Bohan
authors: B
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Xianhui
authors: X
authors: Gao
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Liangjun
authors: L
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors: Honglin
authors: H
authors: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology / Q000648:ultrastructure; D007677:Kidney Function Tests; D008297:Male; D008628:Mercury / Q000652:urine; D017669:Mercury Compounds / Q000633:toxicity*; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D017669:Mercury Compounds; D008628:Mercury; C034211:cinnabar
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