title: Identification of Yellow Pigmentation Genes in Brassica rapa ssp. pekinensis Using Br300 Microarray.
abstract: The yellow color of inner leaves in Chinese cabbage depends on its lutein and carotene content. To identify responsible genes for yellow pigmentation in leaves, the transcriptome profiles of white (Kenshin) and yellow leaves (Wheessen) were examined using the Br300K oligomeric chip in Chinese cabbage. In yellow leaves, genes involved in carotene synthesis (BrPSY, BrPDS, BrCRTISO, and BrLCYE), lutein, and zeaxanthin synthesis (BrCYP97A3 and BrHYDB) were upregulated, while those associated with carotene degradation (BrNCED3, BrNCED4, and BrNCED6) were downregulated. These expression patterns might support that the content of both lutein and total carotenoid was much higher in the yellow leaves than that in the white leaves. These results indicate that the yellow leaves accumulate high levels of both lutein and β-carotene due to stimulation of synthesis and that the degradation rate is inhibited. A large number of responsible genes as novel genes were specifically expressed in yellow inner leaves, suggesting the possible involvement in pigment synthesis. Finally, we identified three transcription factors (BrA20/AN1-like, BrBIM1, and BrZFP8) that are specifically expressed and confirmed their relatedness in carotenoid synthesis from Arabidopsis plants.
authors: Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
authors: Hee-Jeong
authors: HJ
authors: Jung
authors: Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
authors: Ranjith Kumar
authors: RK
authors: Manoharan
authors: Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
authors: Jong-In
authors: JI
authors: Park
authors: Department of Agricultural Education, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
authors: Mi-Young
authors: MY
authors: Chung
authors: Plant Systems Engineering Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Jeongyeo
authors: J
authors: Lee
authors: Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Yong-Pyo
authors: YP
authors: Lim
authors: Department of Biological Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Yoonkang
authors: Y
authors: Hur
authors: Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
authors: Ill-Sup
authors: IS
authors: Nou
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