title: Preparation, chromatographic evaluation and application of adenosine 5'-monophosphate modified ZrO2/SiO2 stationary phase in hydrophilic interaction chromatography.
abstract: The zirconia-coated silica (ZrO2/SiO2) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol-gel technology, to take dual advantages of the suitable porous structure of SiO2 and basic resistance of ZrO2. Adenosine 5'-monophosphate (5'-AMP) was then self-assembled onto ZrO2/SiO2 via Lewis acid-base interaction, generating 5'-AMP-ZrO2/SiO2. The chromatographic properties of 5'-AMP-ZrO2/SiO2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5'-AMP-ZrO2/SiO2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO2/SiO2 and 5'-AMP-ZrO2/SiO2 was superior to the bare ZrO2, and different selectivity was obtained after the introduction of 5'-AMP. For acidic analytes, good resolution was obtained on 5'-AMP-ZrO2/SiO2 while the analysis failed on the bare ZrO2 column owing to strong adsorption. Hence, the proposed 5'-AMP-ZrO2/SiO2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO2 based SP.
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Qing
authors: Q
authors: Wang
authors: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors: Zhi-Yuan
authors: ZY
authors: Luo
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Mao
authors: M
authors: Ye
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Yu-Zhuo
authors: YZ
authors: Wang
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Li
authors: L
authors: Xu
authors: Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address: shizg@whu.edu.cn.
authors: Zhi-Guo
authors: ZG
authors: Shi
authors: Hubei Key Laboratory for Processing and Application of Catalytic Materials, Huanggang Normal University, Huangzhou 438000, China.
authors: Lanying
authors: L
authors: Xu
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007474:Ion Exchange; D009711:Nucleotides / Q000032:analysis / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity; D013449:Sulfonamides / Q000032:analysis / Q000302:isolation & purification; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D009711:Nucleotides; D013449:Sulfonamides; D000249:Adenosine Monophosphate; D012822:Silicon Dioxide; D015040:Zirconium; C028541:zirconium oxide
keywords: Adenosine 5′-monophosphate; Hydrophilic interaction chromatography; Layer-by-layer; ZrO(2)-coated SiO(2)