title: Exploring the microbiota dynamics related to vegetable biomasses degradation and study of lignocellulose-degrading bacteria for industrial biotechnological application.
abstract: The aims of this study were to evaluate the microbial diversity of different lignocellulosic biomasses during degradation under natural conditions and to isolate, select, characterise new well-adapted bacterial strains to detect potentially improved enzyme-producing bacteria. The microbiota of biomass piles of Arundo donax, Eucalyptus camaldulensis and Populus nigra were evaluated by high-throughput sequencing. A highly complex bacterial community was found, composed of ubiquitous bacteria, with the highest representation by the Actinobacteria, Proteobacteria, Bacteroidetes and Firmicutes phyla. The abundances of the major and minor taxa retrieved during the process were determined by the selective pressure produced by the lignocellulosic plant species and degradation conditions. Moreover, cellulolytic bacteria were isolated using differential substrates and screened for cellulase, cellobiase, xylanase, pectinase and ligninase activities. Forty strains that showed multienzymatic activity were selected and identified. The highest endo-cellulase activity was seen in Promicromonospora sukumoe CE86 and Isoptericola variabilis CA84, which were able to degrade cellulose, cellobiose and xylan. Sixty-two percent of bacterial strains tested exhibited high extracellular endo-1,4-ß-glucanase activity in liquid media. These approaches show that the microbiota of lignocellulosic biomasses can be considered an important source of bacterial strains to upgrade the feasibility of lignocellulose conversion for the 'greener' technology of second-generation biofuels.
authors: Department of Agriculture, Division of Microbiology, University of Naples Federico II, Portici, Italy.
authors: Valeria
authors: V
authors: Ventorino
authors: Department of Agriculture, Division of Microbiology, University of Naples Federico II, Portici, Italy.
authors: Alberto
authors: A
authors: Aliberti
authors: Department of Chemical Sciences, University of Naples Federico II, Naples, Italy.
authors: Vincenza
authors: V
authors: Faraco
authors: Department of Agriculture, Division of Microbiology, University of Naples Federico II, Portici, Italy.
authors: Alessandro
authors: A
authors: Robertiello
authors: Department of Chemical Sciences, University of Naples Federico II, Naples, Italy.
authors: Simona
authors: S
authors: Giacobbe
authors: Department of Agriculture, Division of Microbiology, University of Naples Federico II, Portici, Italy.
authors: Danilo
authors: D
authors: Ercolini
authors: Department of Chemical Sciences, University of Naples Federico II, Naples, Italy.
authors: Antonella
authors: A
authors: Amore
authors: Department of Agriculture, Division of Plant Biology and Crop Science, University of Naples Federico II, Portici, Italy.
authors: Massimo
authors: M
authors: Fagnano
authors: Department of Agriculture, Division of Microbiology, University of Naples Federico II, Portici, Italy.
authors: Olimpia
authors: O
authors: Pepe
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D018533:Biomass; D004798:Enzymes / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008031:Lignin / Q000378:metabolism*; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D008031:Lignin
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