title: Wharton's Jelly Transplantation Improves Neurologic Function in a Rat Model of Traumatic Brain Injury.
abstract: Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton's jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight-drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24 h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21-28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI.
authors: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People's Republic of China.
authors: Tian
authors: T
authors: Cheng
authors: Bo
authors: B
authors: Yang
authors: Dongpeng
authors: D
authors: Li
authors: Shanshan
authors: S
authors: Ma
authors: Yi
authors: Y
authors: Tian
authors: Ruina
authors: R
authors: Qu
authors: Wenjin
authors: W
authors: Zhang
authors: Yanting
authors: Y
authors: Zhang
authors: Kai
authors: K
authors: Hu
authors: Fangxia
authors: F
authors: Guan
authors: Jian
authors: J
authors: Wang
mesh_terms: D000818:Animals; D001929:Brain Edema / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D003071:Cognition; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D059631:Wharton Jelly / Q000637:transplantation*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger
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