title: Detection of cyprinid herpesvirus 2 in peripheral blood cells of silver crucian carp, Carassius auratus gibelio (Bloch), suggests its potential in viral diagnosis.
abstract: Epidemics caused by cyprinid herpesvirus 2 (CyHV-2) in domestic cyprinid species have been reported in both European and Asian countries. Although the mechanisms remain unknown, acute CyHV-2 infections generally result in high mortality, and the surviving carps become chronic carriers displaying no external clinical signs. In this study, in situ hybridization analysis showed that CyHV-2 tended to infect peripheral blood cells during either acute or chronic infections in silver crucian carp, Carassius auratus gibelio (Bloch). Laboratory challenge experiments coupled with real-time PCR quantification assays further indicated that steady-state levels of the viral genomic copy number in fish serum exhibited a typical 'one-step' growth curve post-viral challenge. Transcriptional expression of open reading frames (ORF) 121, which was selected due to its highest transcriptional levels in almost all tested tissues, was monitored to represent the replication kinetics of CyHV-2 in peripheral blood cells. Similar kinetic curve of active viral gene transcription in blood cells was obtained as that of serum viral load, indicating that CyHV-2 replicated in peripheral blood cells as well as in other well-characterized tissues. This study should pave the way for designing non-invasive and cost-effective serum diagnostic methods for quick detection of CyHV-2 infection.
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Aquatic Genetic Resources of Ministry of Aquaculture, Shanghai Ocean University, Shanghai, China.
authors: H
authors: H
authors: Wang
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Aquatic Genetic Resources of Ministry of Aquaculture, Shanghai Ocean University, Shanghai, China.
authors: Lj
authors: Lj
authors: Xu
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Aquatic Genetic Resources of Ministry of Aquaculture, Shanghai Ocean University, Shanghai, China.
authors: Lq
authors: L
authors: Lu
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000821:virology*; D002347:Carps / Q000821:virology*; D004279:DNA, Viral / Q000097:blood; D005393:Fish Diseases / Q000175:diagnosis / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006564:Herpesviridae / Q000235:genetics / Q000502:physiology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral
keywords: Carassius auratus gibelio (Bloch); blood cell; cyprinid herpesvirus 2; in situ hybridization; real-time PCR; real-time RT-PCR