title: Reference intervals, intraindividual and interindividual variability, and reference change values for hematologic variables in laboratory beagles.
abstract: In research and development studies for human and veterinary medicine, relevant comparators for interpreting clinical pathology results are matched with concurrent control animals. However, reference intervals (RI) provide a comparator database and important aids for interpreting clinical pathology data, especially in laboratory beagle dogs. Furthermore, RI incorporate biologic variation, which includes analytical, intraindividual, and interindividual variation. No studies to date have established RI and studied the effect of biologic variation on hematologic variables in a large group of laboratory dogs. The purpose of this retrospective study was to establish hematologic RI for laboratory beagles according to international recommendations and estimate the effect of biologic variation in routinely measured hematologic analytes by using the databank at a pharmaceutical center. Blood specimens from 340 healthy beagles (age, 9 to 36 mo) were evaluated by using a flow-cytometry-based hematology analyzer. RI and their 90% confidence intervals were established by using a nonparametric method. Effects of sex, age, and weight were investigated. Weight had no effect on any analyte. RBC, Hgb, Hct, MCV, MCH, RBC distribution width, and platelet count increased with age, whereas WBC count decreased. The only clinically relevant effect of sex was observed for platelets, which were lower in male beagles than in female and warranted 2 different RI. The calculated index of individuality showed that population-based RI were appropriate for almost all hematologic analytes, as might be expected for a homogeneous group of laboratory beagles.
authors: Université de Toulouse, Université Paul Sabatier (UPS), Institut National Polytechnique (INP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Unité Mixte de Service (UMS) 006, Laboratoire Central de Biologie Médicale, Toulouse, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France. n.bourges-abella@envt.fr.
authors: Nathalie H
authors: NH
authors: Bourgès-Abella
authors: SANOFI-AVENTIS Recherche & Développement, Groupe SANOFI, Disposition, Safety and Animal Research, Alfortville, France.
authors: Thierry D
authors: TD
authors: Gury
authors: Université de Toulouse, Université Paul Sabatier (UPS), Institut National Polytechnique (INP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Unité Mixte de Service (UMS) 006, Laboratoire Central de Biologie Médicale, Toulouse, France, Institut National de la Santé et de la Recherche Médicale (INSERM), UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France.
authors: Anne
authors: A
authors: Geffré
authors: Université de Toulouse, INP, ENVT, Unité Mixte de Recherche (UMR) 1331 Toxalim, Toulouse, France.
authors: Didier
authors: D
authors: Concordet
authors: SANOFI-AVENTIS Recherche & Développement, Groupe SANOFI, Disposition, Safety and Animal Research, Alfortville, France.
authors: Kevin C
authors: KC
authors: Thibault-Duprey
authors: SANOFI-AVENTIS Recherche & Développement, Groupe SANOFI, Disposition, Safety and Animal Research, Alfortville, France.
authors: Arnaud
authors: A
authors: Dauchy
authors: Université de Toulouse, Université Paul Sabatier (UPS), Institut National Polytechnique (INP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Unité Mixte de Service (UMS) 006, Laboratoire Central de Biologie Médicale, Toulouse, France, Institut National de la Santé et de la Recherche Médicale (INSERM), UMS 006, Laboratoire Central de Biologie Médicale, Toulouse, France.
authors: Catherine
authors: C
authors: Trumel
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D004285:Dogs / Q000097:blood* / Q000145:classification; D005260:Female; D006403:Hematologic Tests / Q000592:standards / Q000662:veterinary*; D008297:Male; D010976:Platelet Count; D012016:Reference Values; D012189:Retrospective Studies
references: J Small Anim Pract. 2011 Jun;52(6):301-9
references: 21627658
references: Vet Clin Pathol. 2012 Jun;41(2):207-15
references: 22390629
references: Vet Clin Pathol. 2009 Sep;38(3):288-98
references: 19737162
references: Nihon Juigaku Zasshi. 1989 Jun;51(3):636-41
references: 2761161
references: Z Versuchstierkd. 1981;23(5):278-83
references: 7342555
references: Vet Clin Pathol. 2011 Mar;40(1):107-12
references: 21366659
references: Vet Clin Pathol. 1999;28(1):16-19
references: 12075532
references: Vet Clin Pathol. 2011 Sep;40(3):303-15
references: 21884184
references: J Am Vet Med Assoc. 1966 Mar 1;148(5):532-4
references: 5917435
references: J Gerontol. 1965 Apr;20:131-8
references: 14284783
references: Vet Res Commun. 1999 Mar;23(2):73-80
references: 10359151
references: Clin Chem. 1983 Jan;29(1):25-30
references: 6848276
references: Vet Clin Pathol. 2011 Dec;40(4):450-8
references: 22092582
references: Zentralbl Veterinarmed A. 1993 Nov-Dec;40(9-10):720-30
references: 8135079
references: Vet Clin Pathol. 2012 Jun;41(2):175-81
references: 22390475
references: Vet Clin Pathol. 2011 Sep;40(3):324-39
references: 21884185
references: Vet Clin Pathol. 2004;33(1):32-8
references: 15048625
references: Clin Chem Lab Med. 2012 May;50(5):807-12
references: 21958344
references: Vet J. 2007 Jul;174(1):62-8
references: 16815052
references: Clin Chem. 1974 Dec;20(12):1535-42
references: 4430131
references: Clin Chem Lab Med. 2004;42(7):758-64
references: 15327011
references: Clin Chem. 1998 Feb;44(2):327-35
references: 9474032
references: Clin Chem. 1970 Dec;16(12):1022-7
references: 5481562
references: Vet Clin Pathol. 2012 Dec;41(4):441-53
references: 23240820
references: J Am Vet Med Assoc. 1970 Oct 15;157(8):1064-70
references: 5475182
references: Ann Clin Biochem. 2007 Jul;44(Pt 4):343-52
references: 17594781
references: Lab Anim Sci. 1973 Dec;23(6):894-8
references: 4357852
references: J Am Vet Med Assoc. 2003 Nov 15;223(10):1436-42
references: 14627092
references: J Toxicol Environ Health. 1986;18(2):161-88
references: 3712484