title: VvpM induces human cell death via multifarious modes including necroptosis and autophagy.
abstract: VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.
authors: Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea.
authors: Mi-Ae
authors: MA
authors: Lee
authors: Jeong-A
authors: JA
authors: Kim
authors: Mee-Young
authors: MY
authors: Shin
authors: Jeong K
authors: JK
authors: Lee
authors: Soon-Jung
authors: SJ
authors: Park
authors: Kyu-Ho
authors: KH
authors: Lee
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D053148:Caspase 3 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D045726:Metalloproteases / Q000378:metabolism*; D009336:Necrosis; D041261:Vibrio vulnificus / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D007093:Imidazoles; D007211:Indoles; C507699:necrostatin-1; D045304:Cytochromes c; D045726:Metalloproteases; D053148:Caspase 3; D020169:Caspases