title: Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides.
abstract: Gut microbiota influences more physiological and developmental processes of humans and animals than earlier expected. Therefore, the possibility to shape the composition and activity of this bacterial population by prebiotics becomes especially important. Inulin, a β-2,1 linked fructan polymer, from plants and fructooligosaccharides (FOS) derived from it are recognized and already widely used as prebiotics while β-2,6 linked fructans have received much less attention from scientific community. In this mini-review, we will address β-2,6 linked fructans: levan and levan-type FOS as novel potential prebiotics and summarize the literature data on levansucrases of Pseudomonas bacteria which are producing these fructans. The major attention is drawn to stable and highly efficient levansucrases of Pseudomonas syringae pv. tomato, among which the Lsc3 protein has been most thoroughly studied using biochemical methods as well as extensive mutagenesis of the protein.
authors: Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia.
authors: Triinu
authors: T
authors: Visnapuu
authors: Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia.
authors: Karin
authors: K
authors: Mardo
authors: Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia. Electronic address: talamae@ebc.ee.
authors: Tiina
authors: T
authors: Alamäe
mesh_terms: D000818:Animals; D002384:Catalysis; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D009844:Oligosaccharides / Q000096:biosynthesis*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D056692:Prebiotics / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000201:enzymology*
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D056692:Prebiotics; D006602:Hexosyltransferases; C021513:levansucrase