title: Mouse model for pre-clinical study of human cancer immunotherapy.
abstract: This unit describes protocols for developing tumors in mice, including subcutaneous growth, pulmonary metastases of B16 melanoma, and spontaneous melanoma in B-Raf V600E/PTEN deletion transgenic mouse models. Two immunization methods to prevent B16 tumor growth are described using B16.GM-CSF and recombinant vaccinia virus. A therapeutic approach is also included that uses adoptive transfer of tumor antigen-specific T cells. Methods including CTL induction, isolation, testing, and genetic modification of mouse T cells for adoptive transfer by using retrovirus-expressing genes of interest are provided. Additional sections, including growing B16 melanoma, enumerating pulmonary metastases, tumor imaging technique, and use of recombinant viruses for vaccination, are discussed together with safety concerns.
authors: National Cancer Institute, Surgery Branch, Bethesda, Maryland.
authors: Zhiya
authors: Z
authors: Ya
authors: Department of Melanoma Medical Oncology-Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.
authors: Yared
authors: Y
authors: Hailemichael
authors: Department of Melanoma Medical Oncology-Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.
authors: Willem
authors: W
authors: Overwijk
authors: National Cancer Institute, Surgery Branch, Bethesda, Maryland.
authors: Nicholas P
authors: NP
authors: Restifo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D018929:Cell Culture Techniques; D064987:Cell- and Tissue-Based Therapy / Q000009:adverse effects / Q000379:methods; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D007114:Immunization / Q000379:methods; D007167:Immunotherapy / Q000009:adverse effects / Q000379:methods; D008297:Male; D008546:Melanoma, Experimental / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000628:therapy; D051379:Mice; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000276:immunology* / Q000628:therapy*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014161:Transduction, Genetic; D057170:Translational Research, Biomedical; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D019496:Cancer Vaccines; D011948:Receptors, Antigen, T-Cell
keywords: B16 melanoma; translational cancer immunotherapy; tumor imaging; use of recombinant viruses for vaccination
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