title: Electrospun highly ordered mesoporous silica-carbon composite nanofibers for rapid extraction and prefractionation of endogenous peptides.
abstract: A simple method was developed for the preparation of ordered mesoporous silica-carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion-exchange capacity. On the basis of the size-exclusion effect of the mesopores and mixed-mode hydrophobic/ion-exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid-phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three-step elution strategy, followed by LC-MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072 (P.R. China).
authors: Gang-Tian
authors: GT
authors: Zhu
authors: Xi
authors: X
authors: Chen
authors: Xiao-Mei
authors: XM
authors: He
authors: Han
authors: H
authors: Wang
authors: Zheng
authors: Z
authors: Zhang
authors: Yu-Qi
authors: YQ
authors: Feng
mesh_terms: D002244:Carbon / Q000737:chemistry*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D010455:Peptides; D002244:Carbon; D012822:Silicon Dioxide
keywords: electrospinning; ion exchange; mesoporous materials; nanofibers; peptides