title: A conserved regulatory logic controls temporal identity in mouse neural progenitors.
abstract: Neural progenitors alter their output over time to generate different types of neurons and glia in specific chronological sequences, but this process remains poorly understood in vertebrates. Here we show that Casz1, the vertebrate ortholog of the Drosophila temporal identity factor castor, controls the production of mid-/late-born neurons in the murine retina. Casz1 is expressed from mid/late stages in retinal progenitor cells (RPCs), and conditional deletion of Casz1 increases production of early-born retinal neurons at the expense of later-born fates, whereas precocious misexpression of Casz1 has the opposite effect. In both cases, cell proliferation is unaffected, indicating that Casz1 does not control the timing of cell birth but instead biases RPC output directly. Just as Drosophila castor lies downstream of the early temporal identity factor hunchback, we find that the hunchback ortholog Ikzf1 represses Casz1. These results uncover a conserved strategy regulating temporal identity transitions from flies to mammals.
authors: Cellular Neurobiology Research Unit, Institut de recherches cliniques de Montréal (IRCM), Montreal, QC H2W 1R7, Canada.
authors: Pierre
authors: P
authors: Mattar
authors: Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden.
authors: Johan
authors: J
authors: Ericson
authors: The Solomon H. Snyder Department of Neuroscience, Center for High-Throughput Biology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
authors: Seth
authors: S
authors: Blackshaw
authors: Cellular Neurobiology Research Unit, Institut de recherches cliniques de Montréal (IRCM), Montreal, QC H2W 1R7, Canada; Department of Medicine, Université de Montréal, Montreal, QC H3T 1J4, Canada; Department of Anatomy and Cell Biology, and Division of Experimental Medicine, McGill University, Montreal, QC H3A 0G4, Canada. Electronic address: michel.cayouette@ircm.qc.ca.
authors: Michel
authors: M
authors: Cayouette
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D029721:Drosophila Proteins / Q000096:biosynthesis*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000378:metabolism*; D011247:Pregnancy; D055351:Retinal Neurons / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: C511127:CASZ1 protein, human; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors
references: J Biol Chem. 2014 Oct 24;289(43):29801-16
references: 25190801
references: Anat Rec. 1985 Jun;212(2):199-205
references: 3842042
references: Development. 2000 Aug;127(16):3593-605
references: 10903183
references: Nat Rev Neurosci. 2007 Jun;8(6):427-37
references: 17514196
references: Cell. 2009 Nov 25;139(5):969-82
references: 19945380
references: Cell. 2001 Apr 6;105(1):43-55
references: 11301001
references: Genes Dev. 2006 Sep 15;20(18):2618-27
references: 16980589
references: Development. 2005 Jul;132(13):3015-26
references: 15944186
references: Science. 2005 Jun 24;308(5730):1927-30
references: 15976303
references: Genes Dev. 1998 Jan 15;12(2):246-60
references: 9436984
references: Neuron. 2012 Sep 6;75(5):786-98
references: 22958820
references: PLoS Biol. 2004 Sep;2(9):E247
references: 15226823
references: J Neurosci. 2012 Nov 28;32(48):17197-17210
references: 23197712
references: Development. 2005 Nov;132(22):5103-13
references: 16236765
references: Genome Res. 2010 Nov;20(11):1512-25
references: 20693478
references: Nucleic Acids Res. 2009 Jul;37(Web Server issue):W202-8
references: 19458158
references: Cell Stem Cell. 2014 Jun 5;14(6):838-53
references: 24905168
references: Curr Top Dev Biol. 2013;105:69-96
references: 23962839
references: PLoS Genet. 2013;9(2):e1003288
references: 23437007
references: Cell. 2008 May 30;133(5):891-902
references: 18510932
references: Nature. 2013 Jun 27;498(7455):456-62
references: 23783517
references: Cell. 2001 Aug 24;106(4):511-21
references: 11525736
references: Nat Rev Neurosci. 2001 Feb;2(2):109-18
references: 11252990
references: Development. 2011 Jan;138(2):227-35
references: 21148186
references: Development. 2010 Feb;137(4):619-29
references: 20110327
references: Development. 2010 May;137(9):1421-30
references: 20335359
references: Nature. 2011 Jun 15;474(7351):337-42
references: 21677750
references: Neuron. 2008 Oct 9;60(1):26-39
references: 18940586
references: Nature. 2003 Oct 9;425(6958):624-8
references: 14534589
references: Nat Rev Neurosci. 2014 Sep;15(9):615-27
references: 25096185
references: Nat Rev Neurosci. 2013 Dec;14(12):823-38
references: 24400340
references: Dev Cell. 2013 Apr 29;25(2):132-43
references: 23639441
references: Dev Cell. 2008 Apr;14(4):616-23
references: 18410736
references: Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E716-25
references: 23382203
references: Genes Dev. 2006 Feb 15;20(4):429-34
references: 16481472
references: J Comp Neurol. 2004 Jun 21;474(2):304-24
references: 15164429
references: Neural Dev. 2008 Jan 23;3:2
references: 18215319
references: Nature. 2013 Jun 27;498(7455):449-55
references: 23783519