title: MiR-223 suppresses endometrial carcinoma cells proliferation by targeting IGF-1R.
abstract: MicroRNAs were recently found to participate in oncogenesis and growth of various tumors. We hypothesized that microRNA-223 (miR-223) plays a role in endometrial carcinoma growth. In this study, we transfected RL95-2 cells with lentivirus containing miR-223 precursor to establish a miR-223 over-expression model. Proliferation of the cells was greatly inhibited when miR-223 was over-expressed, and cell cycle progress was blocked in G0/G1 phase. To investigate the mechanisms involved, we scanned the putative target genes of miR-223 using bioinformatics, and confirmed that insulin-like growth factor-1 receptor (IGF-1R) was a functional target of miR-223 using quantitative PCR, Western blot and luciferase reporter assay. Meanwhile, over-expressed miR-223 was found to regulate the expression of IGF-1R by repressing protein translation. Silencing IGF-1R with small interfering RNA resulted in similar effect as miR-223 overexpression. Therefore, our data suggest that miR-223 regulates RL95-2 cells proliferation and cell cycle progress by targeting IGF-1R.
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Kai
authors: K
authors: Huang
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Xiyuan
authors: X
authors: Dong
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Cong
authors: C
authors: Sui
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Dan
authors: D
authors: Hu
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Ting
authors: T
authors: Xiong
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Shujie
authors: S
authors: Liao
authors: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, The People's Republic of China.
authors: Hanwang
authors: H
authors: Zhang
keywords: MiR-223; cell proliferation; endometrial carcinoma cell; in vitro; insulin-like growth factor-1 receptor
references: Clin Cancer Res. 2006 Nov 1;12 (21):6373-8
references: 17085648
references: Circulation. 2012 Jun 12;125(23):2892-903
references: 22580331
references: Cell. 2005 Dec 2;123(5):819-31
references: 16325577
references: Int Immunopharmacol. 2011 Feb;11(2):244-9
references: 21145998
references: Eur J Cancer. 2012 Jul;48(10):1570-80
references: 22033326
references: Science. 2004 Jan 2;303(5654):83-6
references: 14657504
references: Expert Opin Ther Targets. 2013 Mar;17(3):307-20
references: 23294364
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Oncogene. 2014 Mar 20;33(12):1601-8
references: 23584479
references: Arch Biochem Biophys. 2012 Dec 15;528(2):204-11
references: 23046980
references: Lancet. 2005 Aug 6-12;366(9484):491-505
references: 16084259
references: Mol Cell Biochem. 2011 Jul;353(1-2):225-33
references: 21442237
references: Gynecol Oncol. 2008 Aug;110(2):206-15
references: 18499237
references: Leuk Res. 2006 May;30(5):643-7
references: 16226311
references: J Intern Med. 2013 Sep;274(3):215-26
references: 23772809
references: Int J Gynecol Cancer. 2012 Jun;22(5):748-54
references: 22635027
references: Gynecol Oncol. 2010 Sep;118(3):251-7
references: 20542546
references: Gynecol Oncol. 2007 Jun;105(3):727-35
references: 17399767
references: Endocr Rev. 1995 Apr;16(2):143-63
references: 7540132
references: Nat Rev Genet. 2004 May;5(5):396-400
references: 15143321
references: Cell. 2004 Jan 23;116(2):281-97
references: 14744438
references: Cancer Cell. 2007 Nov;12(5):457-66
references: 17996649
references: Bioessays. 2002 Feb;24(2):119-29
references: 11835276
references: Am J Obstet Gynecol. 2010 Jun;202(6):656.e1-8
references: 20400061
references: Gynecol Oncol. 1983 Feb;15(1):10-7
references: 6822361
references: Int J Cancer. 2009 Mar 15;124(6):1358-65
references: 19065659
references: Mol Cancer Res. 2011 Jul;9(7):824-33
references: 21628394
references: Mol Cancer. 2008 Apr 28;7:35
references: 18442408
references: Blood. 2010 Mar 4;115(9):1768-78
references: 20029046
references: Science. 2001 Oct 26;294(5543):853-8
references: 11679670
references: Eur J Gynaecol Oncol. 2004;25(2):187-91
references: 15032278
references: PLoS One. 2011;6(11):e27008
references: 22073238
references: Oncol Lett. 2013 Jul;6(1):261-267
references: 23946815
references: J Immunol. 2012 Oct 15;189(8):3795-9
references: 22984081