title: An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids.
abstract: We describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. This is the only identifiable voltage-gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity filter motif (DENS instead of the usual DEKA found in other eukaryotes) may result in a loss of sodium selectivity, as indicated experimentally in mutagenised Drosophila channels.
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom.
authors: Joanna S
authors: JS
authors: Amey
authors: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, United Kingdom; School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, United Kingdom.
authors: Andrias O
authors: AO
authors: O'Reilly
authors: School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, United Kingdom; Department of Cell Physiology and Pharmacology, College of Medicine, Biological Sciences and Psychology, University of Leicester, United Kingdom.
authors: Mark J
authors: MJ
authors: Burton
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom.
authors: Alin M
authors: AM
authors: Puinean
authors: School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, United Kingdom.
authors: Ian R
authors: IR
authors: Mellor
authors: School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, United Kingdom.
authors: Ian R
authors: IR
authors: Duce
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom.
authors: Linda M
authors: LM
authors: Field
authors: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, United Kingdom.
authors: B A
authors: BA
authors: Wallace
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom.
authors: Martin S
authors: MS
authors: Williamson
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom. Electronic address: emyr.davies@rothamsted.ac.uk.
authors: T G Emyr
authors: TG
authors: Davies
mesh_terms: D000818:Animals; D001042:Aphids / Q000378:metabolism*; D019143:Evolution, Molecular; D013779:Tetrodotoxin / Q000378:metabolism; D061566:Voltage-Gated Sodium Channels / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D061566:Voltage-Gated Sodium Channels; D013779:Tetrodotoxin
keywords: Aphid; Evolution; Tetrodotoxin; Voltage-gated sodium channel
references: Mol Biol Evol. 2013 May;30(5):1196-205
references: 23420840
references: J Am Chem Soc. 2014 Mar 5;136(9):3553-9
references: 24517213
references: Cell Rep. 2012 Aug 30;2(2):242-8
references: 22854023
references: J Neurophysiol. 2009 Sep;102(3):1994-2006
references: 19625535
references: Appl Environ Microbiol. 2009 Aug;75(16):5328-35
references: 19542349
references: Nature. 1989 Jun 22;339(6226):597-603
references: 2543931
references: BMC Genomics. 2011;12:56
references: 21261962
references: Mol Biol Evol. 2008 Jun;25(6):1016-24
references: 18258611
references: Biol Lett. 2008 Oct 23;4(5):545-8
references: 18559312
references: Biophys J. 1996 Dec;71(6):3110-25
references: 8968582
references: Insect Biochem Mol Biol. 2011 Sep;41(9):723-32
references: 21640822
references: Bioinformatics. 2010 Oct 1;26(19):2455-7
references: 20671151
references: J Gen Physiol. 2008 Jun;131(6):523-9
references: 18504313
references: Genome Res. 2005 Feb;15(2):330-40
references: 15687296
references: Biophys J. 1998 Dec;75(6):2647-57
references: 9826589
references: PLoS Biol. 2010 Feb;8(2):e1000313
references: 20186266
references: Neuron. 2004 Apr 8;42(1):101-12
references: 15066268
references: Proc Natl Acad Sci U S A. 2012 Jun 26;109 Suppl 1:10619-25
references: 22723361
references: Nature. 2005 Apr 7;434(7034):759-63
references: 15815629
references: J Gen Physiol. 1997 Aug;110(2):119-33
references: 9236205
references: Pestic Biochem Physiol. 2013 Jul 1;106(3):93-100
references: 24019556
references: Science. 1987 Aug 14;237(4816):744-9
references: 2441469
references: Trends Genet. 2005 Jan;21(1):25-30
references: 15680510
references: Syst Biol. 2003 Oct;52(5):696-704
references: 14530136
references: J Biol Chem. 2007 Mar 16;282(11):8044-51
references: 17237232
references: Syst Biol. 2006 Aug;55(4):539-52
references: 16785212
references: Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4556-61
references: 22392995
references: Sci STKE. 2004 Oct 5;2004(253):re15
references: 15467096
references: Nat Rev Genet. 2014 Apr;15(4):272-86
references: 24614317
references: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W609-12
references: 16845082
references: PLoS Genet. 2013;9(3):e1003327
references: 23505382
references: Neuron. 2000 Apr;26(1):13-25
references: 10798388
references: Nature. 2011 Jul 21;475(7356):353-8
references: 21743477
references: Nat Neurosci. 2002 Jun;5(6):511-2
references: 11992118
references: Toxicon. 2013 Mar 1;63:165-83
references: 23261990
references: Curr Biol. 2005 Nov 22;15(22):2069-72
references: 16303569
references: Electrophoresis. 1997 Dec;18(15):2714-23
references: 9504803
references: Insect Biochem Mol Biol. 2005 Mar;35(3):249-56
references: 15705504
references: Insect Biochem Mol Biol. 2012 May;42(5):353-9
references: 22321571
references: Biophys J. 2007 Sep 1;93(5):1519-33
references: 17513361
references: Bioinformatics. 2004 Jul 22;20(11):1806-7
references: 14988107
references: Pflugers Arch. 2009 Sep;458(5):869-76
references: 19475418
references: Curr Biol. 2013 Nov 4;23(21):R948-9
references: 24200318
references: Mar Drugs. 2010;8(2):219-34
references: 20390102
references: Mol Biol Evol. 2005 May;22(5):1208-22
references: 15703242
references: Curr Protoc Protein Sci. 2007 Nov;Chapter 2:Unit 2.9
references: 18429317