title: The 3' addition of CCA to mitochondrial tRNASer(AGY) is specifically impaired in patients with mutations in the tRNA nucleotidyl transferase TRNT1.
abstract: Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1 mutations in two unrelated subjects with different clinical features. The first presented with acute lactic acidosis at 3 weeks of age and developed severe developmental delay, hypotonia, microcephaly, seizures, progressive cortical atrophy, neurosensorial deafness, sideroblastic anemia and renal Fanconi syndrome, dying at 21 months. The second presented at 3.5 years with gait ataxia, dysarthria, gross motor regression, hypotonia, ptosis and ophthalmoplegia and had abnormal signals in brainstem and dentate nucleus. In subject 1, muscle biopsy showed combined oxidative phosphorylation (OXPHOS) defects, but there was no OXPHOS deficiency in fibroblasts from either subject, despite a 10-fold-reduction in TRNT1 protein levels in fibroblasts of the first subject. Furthermore, in normal controls, TRNT1 protein levels are 10-fold lower in muscle than in fibroblasts. High resolution northern blots of subject fibroblast RNA suggested incomplete CCA addition to the non-canonical mitochondrial tRNA(Ser(AGY)), but no obvious qualitative differences in other mitochondrial or cytoplasmic tRNAs. Complete knockdown of TRNT1 in patient fibroblasts rendered mitochondrial tRNA(Ser(AGY)) undetectable, and markedly reduced mitochondrial translation, except polypeptides lacking Ser(AGY) codons. These data suggest that the clinical phenotypes associated with TRNT1 mutations are largely due to impaired mitochondrial translation, resulting from defective CCA addition to mitochondrial tRNA(Ser(AGY)), and that the severity of this biochemical phenotype determines the severity and tissue distribution of clinical features.
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada, Division of Medical Genetics, Department of Pediatrics, and.
authors: Florin
authors: F
authors: Sasarman
authors: Division of Medical Genetics, Department of Pediatrics, and Center for Pediatric Genomic Medicine, Children's Mercy Hospital, Kansas City, MO, USA and.
authors: Isabelle
authors: I
authors: Thiffault
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada.
authors: Woranontee
authors: W
authors: Weraarpachai
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada.
authors: Steven
authors: S
authors: Salomon
authors: Division of Medical Genetics, Department of Pediatrics, and.
authors: Catalina
authors: C
authors: Maftei
authors: Division of Medical Genetics, Department of Pediatrics, and.
authors: Julie
authors: J
authors: Gauthier
authors: Department of Pathology, CHU Sainte-Justine and Université de Montréal, Montreal, QC, Canada.
authors: Benjamin
authors: B
authors: Ellazam
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada, Division of Medical Genetics, Department of Pediatrics, and.
authors: Neil
authors: N
authors: Webb
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada.
authors: Hana
authors: H
authors: Antonicka
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada.
authors: Alexandre
authors: A
authors: Janer
authors: Division of Medical Genetics, Department of Pediatrics, and.
authors: Catherine
authors: C
authors: Brunel-Guitton
authors: Monique and Jacques Roboh Department of Genetic Research, Hadassah, Hebrew University Medical Center, Jerusalem, Israel.
authors: Orly
authors: O
authors: Elpeleg
authors: Division of Medical Genetics, Department of Pediatrics, and.
authors: Grant
authors: G
authors: Mitchell
authors: Montreal Neurological Institute and Department of Human Genetics, McGill University, Montreal, QC, Canada, eric@ericpc.mni.mcgill.ca.
authors: Eric A
authors: EA
authors: Shoubridge
mesh_terms: D002648:Child; D002675:Child, Preschool; D059472:Exome; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000235:genetics*; D009154:Mutation; D014176:Protein Biosynthesis / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D012362:RNA, Transfer, Ser / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013577:Syndrome
chemical_list: D012362:RNA, Transfer, Ser; D012316:RNA Nucleotidyltransferases; C023198:tRNA nucleotidyltransferase
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