title: Establishment and evaluation of a murine ανβ3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus.
abstract: Integrin ανβ3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of ανβ3 integrin, a stable CHO-677 cell line expressing the murine ανβ3 heterodimer (designated as "CHO-677-mανβ3" cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-mανβ3 cell line at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed stably. A plaque-forming assay, 50% tissue culture infective dose (TCID50), real-time quantitative reverse transcription-PCR, and IFA were used to detect the replication levels of Foot-and-mouth disease virus (FMDV) in the CHO-677-mανβ3 cell line. After infection with FMDV/O/ZK/93, the cell line showed a significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stable ανβ3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of ανβ3 integrin, and as a cell model for FMDV research.
authors: State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors: Wei
authors: W
authors: Zhang
authors: Kaiqi
authors: K
authors: Lian
authors: Fan
authors: F
authors: Yang
authors: Yang
authors: Y
authors: Yang
authors: Zhijian
authors: Z
authors: Zhu
authors: Zixiang
authors: Z
authors: Zhu
authors: Weijun
authors: W
authors: Cao
authors: Ruoqing
authors: R
authors: Mao
authors: Ye
authors: Y
authors: Jin
authors: Jijun
authors: J
authors: He
authors: Jianhong
authors: J
authors: Guo
authors: Xiangtao
authors: X
authors: Liu
authors: Haixue
authors: H
authors: Zheng
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D016466:CHO Cells; D003001:Cloning, Molecular; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004198:Disease Susceptibility / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000235:genetics* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000502:physiology*; D039302:Integrin alphaVbeta3 / Q000235:genetics* / Q000378:metabolism; D051379:Mice
chemical_list: D018076:DNA, Complementary; D039302:Integrin alphaVbeta3
keywords: Foot-and-mouth disease virus; cell line; integrin; receptor; ανβ3
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