title: Characterization of porcine partially reprogrammed iPSCs from adipose-derived stem cells.
abstract: Partially reprogrammed induced pluripotent stem cells (PiPSCs) have great potential for investigating reprogramming mechanisms and represent an alternative potential material for making genetically modified animals and regenerative medicine. To date, PiPSCs have scarcely been reported in detail when compared with mice and humans. In this study, we obtained PiPSCs from porcine adipose-derived stem cells (pADSCs) by ectopic expression of human transcription factors (OCT4, SOX2, c-MYC, and KLF4) in feeder-free condition. The morphology and proliferation activity of porcine PiPSCs (pPiPSCs) were similar to those of porcine fully reprogrammed iPSCs (pFiPSCs); furthermore, pPiPSCs expressed higher levels of the typical surface molecules (CD29) found in pADSCs. However, pPiPSCs were negative for key proteins (NANOG) connected with stemness and possessed lower differentiation ability in vivo and in vitro. When differentiation-inhibiting factors were withdrawn, pPiPSCs-derived cells (pPiPSC-DCs) showed similar features to pADSCs in many aspects, including proliferation, differentiation, and immunosuppression. When both types of cells were used to produce cloned embryos, we found that the blastocyst formation rate of 19DC (one of the pPiPSC-DC cell lines)-derived cloned embryos was obviously higher than that of others. The total cell number of 19DC-derived blastocysts was significantly higher than the 30DC (one pFiPSC-DC cell line)-derived blastocysts. In all, through limited differentiation ability, the proliferation activity of pPiPSCs is similar to that of pFiPSCs, and pPiPSCs can retain several of the features of pADSCs, which are beneficial to cell therapy. Furthermore, the differentiation of pPiPSCs is more favorable for producing high-quality reconstructed embryos.
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Chao
authors: C
authors: Wei
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Xia
authors: X
authors: Li
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Pengfei
authors: P
authors: Zhang
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Yu
authors: Y
authors: Zhang
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Tong
authors: T
authors: Liu
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Shaoshuai
authors: S
authors: Jiang
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors: Fei
authors: F
authors: Han
authors: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and BreedingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China yunhaizhang@ahau.edu.cn.
authors: Yunhai
authors: Y
authors: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000502:physiology*; D058732:Embryoid Bodies / Q000166:cytology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells; D006801:Humans; D007124:Immunoenzyme Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013552:Swine
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D012333:RNA, Messenger