title: Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects.
abstract: The purpose of the present study was to determine whether probiotic supplementation (Lactobacillus casei Shirota (LcS)) prevents diet-induced insulin resistance in human subjects. A total of seventeen healthy subjects were randomised to either a probiotic (n 8) or a control (n 9) group. The probiotic group consumed a LcS-fermented milk drink twice daily for 4 weeks, whereas the control group received no supplementation. Subjects maintained their normal diet for the first 3 weeks of the study, after which they consumed a high-fat (65 % of energy), high-energy (50 % increase in energy intake) diet for 7 d. Whole-body insulin sensitivity was assessed by an oral glucose tolerance test conducted before and after overfeeding. Body mass increased by 0·6 (SE 0·2) kg in the control group (P< 0·05) and by 0·3 (SE 0·2) kg in the probiotic group (P>0·05). Fasting plasma glucose concentrations increased following 7 d of overeating (control group: 5·3 (SE 0·1) v. 5·6 (SE 0·2) mmol/l before and after overfeeding, respectively, P< 0·05), whereas fasting serum insulin concentrations were maintained in both groups. Glucose AUC values increased by 10 % (from 817 (SE 45) to 899 (SE 39) mmol/l per 120 min, P< 0·05) and whole-body insulin sensitivity decreased by 27 % (from 5·3 (SE 1·4) to 3·9 (SE 0·9), P< 0·05) in the control group, whereas normal insulin sensitivity was maintained in the probiotic group (4·4 (SE 0·8) and 4·5 (SE 0·9) before and after overeating, respectively (P>0·05). These results suggest that probiotic supplementation may be useful in the prevention of diet-induced metabolic diseases such as type 2 diabetes.
authors: School of Sport, Exercise and Health Sciences, Loughborough University,Loughborough,LeicestershireLE11 3TU,UK.
authors: Carl J
authors: CJ
authors: Hulston
authors: School of Sport, Exercise and Health Sciences, Loughborough University,Loughborough,LeicestershireLE11 3TU,UK.
authors: Amelia A
authors: AA
authors: Churnside
authors: MRC Human Nutrition Research, Elsie Widdowson Laboratory,Fulbourn Road,CambridgeCB1 9NL,UK.
authors: Michelle C
authors: MC
authors: Venables
mesh_terms: D000328:Adult; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D043302:Cultured Milk Products / Q000382:microbiology*; D059305:Diet, High-Fat / Q000009:adverse effects; D002149:Energy Intake; D004739:England; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D006943:Hyperglycemia / Q000209:etiology / Q000517:prevention & control*; D006963:Hyperphagia / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007780:Lacticaseibacillus casei; D008297:Male; D050177:Overweight / Q000209:etiology / Q000517:prevention & control*; D019936:Probiotics / Q000627:therapeutic use*; D015430:Weight Gain; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin
keywords: Probiotics
references: Diabetes Care. 2012 Feb;35(2):375-82
references: 22210577
references: Br J Nutr. 2014 May 28;111(10):1853-61
references: 24506887
references: Diabetes Care. 2011 Feb;34(2):392-7
references: 21270197
references: Am J Clin Nutr. 2008 May;87(5):1219-23
references: 18469242
references: Am J Physiol Endocrinol Metab. 2007 Feb;292(2):E485-93
references: 17003244
references: J Appl Microbiol. 2011 Mar;110(3):650-7
references: 21281408
references: Int J Clin Pract Suppl. 2004 Oct;(143):9-21
references: 16035392
references: Diabetes Care. 1999 Sep;22(9):1462-70
references: 10480510
references: Diabetes. 2010 Jan;59(1):172-81
references: 19794059
references: Gut. 2009 Aug;58(8):1091-103
references: 19240062
references: Am J Clin Nutr. 1995 Nov;62(5):988-95
references: 7572747
references: Am J Physiol Endocrinol Metab. 2000 Jun;278(6):E977-84
references: 10826998
references: Gut. 2012 Apr;61(4):543-53
references: 22110050
references: J Biol Chem. 2002 Dec 27;277(52):50230-6
references: 12006582
references: J Clin Invest. 1996 May 1;97(9):2081-91
references: 8621798
references: Diabetes. 2007 Jul;56(7):1856-64
references: 17440174
references: Science. 1996 Feb 2;271(5249):665-8
references: 8571133
references: J Dairy Sci. 2013 Jan;96(1):89-95
references: 23164226
references: Gastroenterology. 2012 May;142(5):1100-1101.e2
references: 22326433
references: Curr Opin Lipidol. 2002 Feb;13(1):33-40
references: 11790961
references: Nutrition. 2012 May;28(5):539-43
references: 22129852
references: PLoS One. 2010;5(2):e9085
references: 20140211
references: Diabetes. 2008 Jun;57(6):1470-81
references: 18305141
references: J Biosci Bioeng. 2010 Nov;110(5):547-52
references: 20580604
references: Am J Physiol. 1989 Feb;256(2 Pt 1):E242-9
references: 2645785
references: Diabetes. 2001 Aug;50(8):1901-10
references: 11473054
references: Am J Physiol Gastrointest Liver Physiol. 2007 Feb;292(2):G518-25
references: 17023554
references: Am J Physiol Endocrinol Metab. 2013 Aug 1;305(3):E388-95
references: 23736545
references: Br J Nutr. 2011 Aug;106(4):549-56
references: 21521545
references: Eur J Clin Nutr. 1998 Dec;52(12):899-907
references: 9881885
references: Endocr J. 1997 Jun;44(3):357-65
references: 9279510
references: Nutr Metab (Lond). 2009 Sep 28;6:37
references: 19781106
references: Am J Physiol Endocrinol Metab. 2013 Nov 1;305(9):E1059-70
references: 23982154
references: Appl Physiol Nutr Metab. 2013 May;38(5):512-9
references: 23668758
references: J Physiol. 2009 May 15;587(Pt 10):2387-97
references: 19332493
references: Diabetes. 2007 Jul;56(7):1761-72
references: 17456850
references: J Diabetes Investig. 2014 Mar 23;5(2):153-61
references: 24843755
references: APMIS. 1997 Aug;105(8):643-9
references: 9298104
references: Am J Physiol Endocrinol Metab. 2013 May 15;304(10):E1053-63
references: 23531614
references: PLoS One. 2010;5(1):e8817
references: 20098690
references: Cell. 2012 Mar 2;148(5):852-71
references: 22385956