title: Hydrophobic cyanine dye-doped micelles for optical in vivo imaging of plasma leakage and vascular disruption.
abstract: Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats. The imaging formula is stable and exerts no acute toxic effects in vitro. It accumulated specifically in the inflamed regions in all models, which could be demonstrated by both conventional epifluorescence imaging, and fluorescence tomography both as a standalone technique and also by merging it with computed tomography scans. Ex vivo verification of dye accumulation by confocal fluorescence microscopy was also possible. The present formula allows sensitive and specific detection of inflammatory plasma leakage in diverse models. Its potential for imaging larger animals was also demonstrated. IR-676-doped micelles offer an excellent opportunity to image inflammatory vascular leakage in various models and species.
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Bálint
authors: B
authors: Botz
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Kata
authors: K
authors: Bölcskei
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Ágnes
authors: Á
authors: Kemény
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, Hungary.
authors: Zoltán
authors: Z
authors: Sándor
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Valéria
authors: V
authors: Tékus
authors: University of Pécs, School of Medicine, Department of Medical Biology, Szigeti út 12, Pécs H-7624, HungarydUniversity of Pécs, János Szentágothai Research Centre, Signal Transduction Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: György
authors: G
authors: Sétáló
authors: Semmelweis University School of Medicine and MTA-SE "Lendület" Inflammation Physiology Research Group, Department of Physiology, Tuzoltó u. 37-47, Budapest H-1094, Hungary.
authors: Janka
authors: J
authors: Csepregi
authors: Semmelweis University School of Medicine and MTA-SE "Lendület" Inflammation Physiology Research Group, Department of Physiology, Tuzoltó u. 37-47, Budapest H-1094, Hungary.
authors: Attila
authors: A
authors: Mócsai
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Erika
authors: E
authors: Pintér
authors: University of Pécs, Department of Inorganic Chemistry, Ifjúság útja. 6, Pécs H-7624, Hungary.
authors: László
authors: L
authors: Kollár
authors: University of Pécs, School of Medicine, Department of Pharmacology and Pharmacotherapy, Szigeti út 12, Pécs H-7624, HungarybUniversity of Pécs, János Szentágothai Research Centre, Molecular Pharmacology Research Team, Ifjúság útja 20, Pécs H-7624, Hungary.
authors: Zsuzsanna
authors: Z
authors: Helyes
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism; D004487:Edema / Q000473:pathology; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008823:Micelles; D061848:Optical Imaging / Q000379:methods*; D011014:Pneumonia / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: D001798:Blood Proteins; D005456:Fluorescent Dyes; D008823:Micelles