title: MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort.
abstract: The aim of this study was to investigate the expression of circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE(-/-)) and to validate the role of these miRNAs in human coronary artery disease (CAD). Pooled plasma from 10 apoE(-/-) mice and 10 healthy C57BL/6 (B6) mice was used to perform the microarray analysis. The results showed that miR-34a, miR-21, miR-23a, miR-30a and miR-106b were differentially expressed in apoE(-/-) mice, and these expression changes were confirmed by real-time quantitative reverse-transcription PCR. Then, miR-34a, miR-21, miR-23a, miR-30a and miR-106b were detected in the plasma of 32 patients with CAD and of 20 healthy controls. Only miR-34a, miR-21 and miR-23a were significantly differentially expressed in the plasma of CAD patients (all P<0.01). In conclusion, miR-34a, miR-21 and miR-23a were elevated in CAD patients, which means that these miRNAs might serve as biomarkers of CAD development and progression.
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Hui
authors: H
authors: Han
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Guangjin
authors: G
authors: Qu
authors: Department of Natural Product Chemistry, the Daqing Campus of Harbin Medical University, Daqing, China.
authors: Chenghua
authors: C
authors: Han
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Yuhong
authors: Y
authors: Wang
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Tingting
authors: T
authors: Sun
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Fengqing
authors: F
authors: Li
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Junxiao
authors: J
authors: Wang
authors: Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
authors: Shanshun
authors: S
authors: Luo
mesh_terms: D000368:Aged; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency; D015415:Biomarkers; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010865:Pilot Projects; D015203:Reproducibility of Results; D012307:Risk Factors
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; C524940:MIRN21 microRNA, human; C552430:MIRN23a microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs
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