title: Analysis of RNA from Alzheimer's Disease Post-mortem Brain Tissues.
abstract: Alzheimer's disease (AD) is a uniquely human, age-related central nervous system (CNS) disorder for which there is no adequate experimental model. While well over 100 transgenic murine models of AD (TgAD) have been developed that recapitulate many of the neuropathological features of AD, key pathological features of AD such as progressive neuronal atrophy, neuron cell loss, and neurofibrillary tangle (NFT) formation have not been observed in any TgAD model to date. To more completely analyze and understand the neuropathology, altered neuro-inflammatory and innate-immune signaling pathways, and the complex molecular-genetics and epigenetics of AD, it is therefore necessary to rigorously examine short post-mortem interval (PMI) human brain tissues to gain a deeper and more thorough insight into the neuropathological mechanisms that characterize the AD process. This perspective-methods paper will highlight some important recent findings on the utilization of short PMI tissues in sporadic (idiopathic; of unknown origin) AD research with focus on the extraction and quantification of RNA, and in particular microRNA (miRNA) and messenger RNA (mRNA) and analytical strategies, drawing on the authors' combined 125 years of laboratory experience into this investigative research area. We sincerely hope that new investigators in the field of "gene expression analysis in neurological disease" will benefit from the observations presented here and incorporate these recent findings and observations into their future experimental planning and design.
authors: Infectious Diseases, Experimental Therapeutics and Human Toxicology Lab, Southern University at New Orleans, New Orleans, LA, 70126, USA.
authors: Christian
authors: C
authors: Clement
authors: LSU Department of Ophthalmology, Louisiana State University Health Science Center, New Orleans, LA, 70112, USA.|LSU Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA, 70112, USA.
authors: James M
authors: JM
authors: Hill
authors: Department of Health Information Management, Louisiana State University, Ruston, LA, 71270, USA.
authors: Prerna
authors: P
authors: Dua
authors: Department of Neurosurgery, Louisiana State University Health Science Center, New Orleans, LA, 70112, USA.
authors: Frank
authors: F
authors: Culicchia
authors: LSU Department of Ophthalmology, Louisiana State University Health Science Center, New Orleans, LA, 70112, USA. wlukiw@lsuhsc.edu.|LSU Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA, 70112, USA. wlukiw@lsuhsc.edu.|Department of Neurology, Louisiana State University Health Science Center, New Orleans, LA, 70112, USA. wlukiw@lsuhsc.edu.
authors: Walter J
authors: WJ
authors: Lukiw
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D064307:Microbiota; D011180:Postmortem Changes; D012313:RNA / Q000235:genetics* / Q000378:metabolism
chemical_list: D012313:RNA
keywords: Agonal processes; Brain death; DNA arrays; Human brain gene expression; Microbiome; Post-mortem tissues; RNA instability; Run-on transcription; Sample pooling; Thanatomicrobiome; miRNA arrays; miRNA-146a; miRNA-34a; miRNA-9
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