title: iMiRNA-PseDPC: microRNA precursor identification with a pseudo distance-pair composition approach.
abstract: A microRNA (miRNA) is a small non-coding RNA molecule, functioning in transcriptional and post-transcriptional regulation of gene expression. The human genome may encode over 1000 miRNAs. Albeit poorly characterized, miRNAs are widely deemed as important regulators of biological processes. Aberrant expression of miRNAs has been observed in many cancers and other disease states, indicating that they are deeply implicated with these diseases, particularly in carcinogenesis. Therefore, it is important for both basic research and miRNA-based therapy to discriminate the real pre-miRNAs from the false ones (such as hairpin sequences with similar stem-loops). Particularly, with the avalanche of RNA sequences generated in the post-genomic age, it is highly desired to develop computational sequence-based methods for effectively identifying the human pre-miRNAs. Here, we propose a predictor called "iMiRNA-PseDPC", in which the RNA sequences are formulated by a novel feature vector called "pseudo distance-pair composition" (PseDPC) with 10 types of structure statuses. Rigorous cross-validations on a much larger and more stringent newly constructed benchmark data-set showed that our approach has remarkably outperformed the existing ones in either prediction accuracy or efficiency, indicating the new predictor is quite promising or at least may become a complementary tool to the existing predictors in this area. For the convenience of most experimental scientists, a user-friendly web server for the new predictor has been established at http://bioinformatics.hitsz.edu.cn/iMiRNA-PseDPC/, by which users can easily get their desired results without the need to go through the mathematical details. It is anticipated that the new predictor may become a useful high throughput tool for genome analysis particularly in dealing with large-scale data.
authors: a School of Computer Science and Technology , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong 518055 , China.|b Key Laboratory of Network Oriented Intelligent Computation , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong , China.|c Gordon Life Science Institute , Belmont , MA 02478 , USA.
authors: Bin
authors: B
authors: Liu
authors: a School of Computer Science and Technology , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong 518055 , China.
authors: Longyun
authors: L
authors: Fang
authors: a School of Computer Science and Technology , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong 518055 , China.
authors: Fule
authors: F
authors: Liu
authors: a School of Computer Science and Technology , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong 518055 , China.|b Key Laboratory of Network Oriented Intelligent Computation , Harbin Institute of Technology Shenzhen Graduate School , Shenzhen , Guangdong , China.
authors: Xiaolong
authors: X
authors: Wang
authors: c Gordon Life Science Institute , Belmont , MA 02478 , USA.|d Center of Excellence in Genomic Medicine Research (CEGMR) , King Abdulaziz University , Jeddah , Saudi Arabia.
authors: Kuo-Chen
authors: KC
authors: Chou
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D020407:Internet; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics; D009690:Nucleic Acid Conformation; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software
chemical_list: D035683:MicroRNAs
keywords: Chou’s PseAAC approach; free energy; iMiRNA-PseDPC; large-scale analysis; local structure status; pre-miRNA