title: Dual-labeled time-resolved immunofluorometric assay for the determination of IgM antibodies to rubella virus and cytomegalovirus in human serum.
abstract: OBJECTIVE
  This study established a novel time-resolved fluorescence immunoassay (TRFIA) that allows the simultaneous determination of rubella virus (RV) IgM and cytomegalovirus (CMV) IgM in human serum.
  
  
  METHODS
  Lanthanum elements labeled antibody and streptavidin-biotin system were used in the "capture sandwich" format simultaneously.
  
  
  RESULTS
  The working range of TRFIA for RV IgM was 2-80 AU/mL and for CMV IgM was 5-400 AU/mL. Intra- and inter-assay coefficient of variation (CV) for RV IgM and CMV IgM were both less than 10% and recoveries were from 90% to 110%. No significant statistical difference in sensitivity or specificity was observed between dual-TRFIA and commercial chemiluminescent immunoassays (CLIA) in serum samples.
  
  
  CONCLUSIONS
  The novel dual-TRFIA for RV IgM and CMV IgM detection might have valuable clinical application, with satisfactory sensitivity, specificity and accuracy.
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Jian-Wei
authors: JW
authors: Zhou
authors: Institute of Hydrobiology, Jinan University, Guangzhou 510515, Guangdong, PR China.
authors: La-Mei
authors: LM
authors: Lei
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Qian-Ni
authors: QN
authors: Liang
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Tian-Cai
authors: TC
authors: Liu
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Guan-Feng
authors: GF
authors: Lin
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Zhi-Ning
authors: ZN
authors: Dong
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Rong-Liang
authors: RL
authors: Liang
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
authors: Zhen-Hua
authors: ZH
authors: Chen
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, PR China. Electronic address: wg@smu.edu.cn.
authors: Ying-Song
authors: YS
authors: Wu
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012412:Rubella virus / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M
keywords: Cytomegalovirus; Dual-TRFIA; IgM; Rubella virus