title: Inkjet-bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging.
abstract: Inkjet bioprinting is one of the most promising additive manufacturing approaches for tissue fabrication with the advantages of high speed, high resolution, and low cost. The limitation of this technology is the potential damage to the printed cells and frequent clogging of the printhead. Here we developed acrylated peptides and co-printed with acrylated poly(ethylene glycol) (PEG) hydrogel with simultaneous photopolymerization. At the same time, the bone marrow-derived human mesenchymal stem cells (hMSCs) were precisely printed during the scaffold fabrication process so the cells were delivered simultaneously with minimal UV exposure. The multiple steps of scaffold synthesis and cell encapsulation were successfully combined into one single step using bioprinting. The resulted peptide-conjugated PEG scaffold demonstrated excellent biocompatibility, with a cell viability of 87.9 ± 5.3%. Nozzle clogging was minimized due to the low viscosity of the PEG polymer. With osteogenic and chondrogenic differentiation, the bioprinted bone and cartilage tissue demonstrated excellent mineral and cartilage matrix deposition, as well as significantly increased mechanical properties. Strikingly, the bioprinted PEG-peptide scaffold dramatically inhibited hMSC hypertrophy during chondrogenic differentiation. Collectively, bioprinted PEG-peptide scaffold and hMSCs significantly enhanced osteogenic and chondrogenic differentiation for robust bone and cartilage formation with minimal printhead clogging.
authors: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, Hubei, China.|Stemorgan Therapeutics, Albany, NY, USA.
authors: Guifang
authors: G
authors: Gao
authors: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.|Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan.
authors: Tomo
authors: T
authors: Yonezawa
authors: Stemorgan Therapeutics, Albany, NY, USA.
authors: Karen
authors: K
authors: Hubbell
authors: Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA. daig@rpi.edu.
authors: Guohao
authors: G
authors: Dai
authors: Stemorgan Therapeutics, Albany, NY, USA. xfc.cui@gmail.com.|Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA. xfc.cui@gmail.com.
authors: Xiaofeng
authors: X
authors: Cui
mesh_terms: D062028:Bioprinting / Q000379:methods*; D001846:Bone Development / Q000235:genetics; D002356:Cartilage / Q000254:growth & development*; D002454:Cell Differentiation / Q000235:genetics; D020219:Chondrogenesis / Q000235:genetics*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D010455:Peptides; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols
keywords: Bioprinting; Cartilage; Extracellular matrix; Mesenchymal stem cells; Photopolymerization