title: Solanesol protects human hepatic L02 cells from ethanol-induced oxidative injury via upregulation of HO-1 and Hsp70.
abstract: In present study, we showed that the mRNA and protein levels of HO-1 and Hsp70 in solanesol-treated L02 cells were significantly increased. The induction of the HO-1 by solanesol is majorly achieved via enhancing the nuclear translocation and transactivity of Nrf2 through enhancement of Hsp90-Keap1 interaction, while solanesol-elevated Hsp70 is related with promoting the nuclear translocation of HSF1 through the involvement of chaperones interaction. Furthermore, the induction of HO-1 and Hsp70 by solanesol could protect against ethanol-induced liver injury, including significantly suppressing the elevation of the activities of LDH and AST, attenuating ethanol-induced increase of the MDA, ROS level and decrease of the GSH level. Moreover, solanesol also suppressed ethanol-induced apoptosis of L02 cells by inhibition of nuclear morphological damage, procaspase 3 and cleavage of caspase 3 and PARP, suggesting solanesol may be beneficial against ALD. Solanesol also promoted tBHQ-mediated protective effects. However, treatment cells with SnPP or PES markedly abrogated the protective effects of solanesol on ethanol-induced cell injury. These results strongly suggested that solanesol could protect ethanol-induced L02 cell damage, which might be attributed to the activation of HO-1 and Hsp70.
authors: Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China. Electronic address: leeyxy@gmail.com.
authors: Xiangyang
authors: X
authors: Yao
authors: Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China.
authors: Qin
authors: Q
authors: Bai
authors: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, PR China.
authors: Dazhong
authors: D
authors: Yan
authors: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, PR China.
authors: Guilan
authors: G
authors: Li
authors: Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China.
authors: Chaotian
authors: C
authors: Lü
authors: Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China.
authors: Hui
authors: H
authors: Xu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D000431:Ethanol / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D008099:Liver / Q000473:pathology; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D002492:Central Nervous System Depressants; D018840:HSP70 Heat-Shock Proteins; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D020011:Protective Agents; D013729:Terpenes; D000431:Ethanol; D051547:Heme Oxygenase-1; C017719:solanesol
keywords: HO-1; HSF1; Hsp70; Nrf2; Solanesol