title: MMP2 and MMP9 participate in S1P-induced invasion of follicular ML-1 thyroid cancer cells.
abstract: The bioactive lipid sphingosine-1-phosphate (S1P) has emerged as a potent inducer of cancer cell migration and invasion. Previously, we have shown that S1P induces invasion of ML-1 follicular thyroid cancer cells via S1P receptors 1 and 3 (S1P1,3). Matrix metalloproteinases (MMPs) are zinc-dependent proteolytic enzymes used by cells for degradation of the extracellular matrix during invasion and migration. In the present study, we examined the role of MMP2 and MMP9 for S1P-induced invasion of ML-1 cells, and found that S1P regulates the secretion and activity of MMP2 and MMP9 via S1P1,3. Both pharmacological inhibitors and siRNA knockdown of MMP2 and MMP9 could attenuate S1P-induced invasion. Additionally, we show that calpains and Rac1 mediate S1P-induced secretion of MMP2 and MMP9. In conclusion, MMP2 and MMP9 participate in S1P-evoked follicular ML-1 thyroid cancer cell invasion.
authors: Department of Biosciences, Åbo Akademi University, Biocity, Artillerigatan 6, Turku 20520, Finland; The Minerva Foundation Institute for Medical Research, Biomedicum Helsinki, Helsinki 00290, Finland.
authors: Veronica
authors: V
authors: Kalhori
authors: Department of Biosciences, Åbo Akademi University, Biocity, Artillerigatan 6, Turku 20520, Finland; The Minerva Foundation Institute for Medical Research, Biomedicum Helsinki, Helsinki 00290, Finland. Electronic address: ktornqvi@bo.fi.
authors: Kid
authors: K
authors: Törnquist
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000201:enzymology / Q000473:pathology*; D002154:Calpain / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013964:Thyroid Neoplasms / Q000201:enzymology / Q000473:pathology*; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; C107953:RAC1 protein, human; C060506:sphingosine 1-phosphate; D002154:Calpain; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D020830:rac1 GTP-Binding Protein; D013110:Sphingosine
keywords: Calpain; MMP2; MMP9; Migration and invasion; Sphingosine 1-phosphate; Thyroid cancer