title: Antibacterial activity and biological performance of a novel antibacterial coating containing a halogenated furanone compound loaded poly(L-lactic acid) nanoparticles on microarc-oxidized titanium.
abstract: Titanium implants have been widely used for many medical applications, but bacterial infection after implant surgery remains one of the most common and intractable complications. To this end, long-term antibacterial ability of the implant surface is highly desirable to prevent implant-associated infection. In this study, a novel antibacterial coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone loaded poly(L-lactic acid) nanoparticles, was fabricated on microarc-oxidized titanium for this purpose. The antibacterial coating produced a unique inhibition zone against Staphylococcus aureus throughout a 60-day study period, which is normally long enough to prevent the infection around implants in the early and intermediate stages. The antibacterial rate for adherent S. aureus was about 100% in the first 10 days and constantly remained over 90% in the following 20 days. Fluorescence staining of adherent S. aureus also confirmed the excellent antibacterial ability of the antibacterial coating. Moreover, in vitro experiments showed an enhanced osteoblast adhesion and proliferation on the antibacterial coating, and more notable cell spread was observed at the early stage. It is therefore concluded that the fabricated antibacterial coating, which exhibits relatively long-term antibacterial ability and excellent biological performance, is a potential and promising strategy to prevent implant-associated infection.
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China ; Department of Stomatology, Jingdu Hospital, Nanjing, People's Republic of China.
authors: Yicheng
authors: Y
authors: Cheng
authors: Institute of Neuroscience, School of Basic Medicine, Fourth Military Medical University, Xi'an, People's Republic of China.
authors: Xianghui
authors: X
authors: Zhao
authors: Department of Stomatology, Jingdu Hospital, Nanjing, People's Republic of China.
authors: Xianghui
authors: X
authors: Liu
authors: Department of Stomatology, Jingdu Hospital, Nanjing, People's Republic of China.
authors: Weige
authors: W
authors: Sun
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors: Huifang
authors: H
authors: Ren
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors: Bo
authors: B
authors: Gao
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors: Jiang
authors: J
authors: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005663:Furans / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*; D014025:Titanium / Q000737:chemistry*
chemical_list: C494387:3-(1'-bromohexyl)-5-dibromomethylene-2(5H)-furanone; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005663:Furans; D011091:Polyesters; D011108:Polymers; C009495:titanium dioxide; D019344:Lactic Acid; C033616:poly(lactide); D014025:Titanium
keywords: microarc oxidation; osteoblasts
references: Chem Soc Rev. 2006 Sep;35(9):780-9
references: 16936926
references: J Mater Sci Mater Med. 1999 Aug;10(8):453-7
references: 15348111
references: Biomaterials. 2004 Aug;25(17):3341-9
references: 15020106
references: J Control Release. 2004 Sep 14;99(1):127-37
references: 15342186
references: N Engl J Med. 2004 Apr 1;350(14):1422-9
references: 15070792
references: J Biomed Mater Res A. 2006 Aug;78(2):247-57
references: 16628710
references: Biomaterials. 2004 Sep;25(20):5003-12
references: 15109862
references: Pharm Res. 2003 Feb;20(2):149-52
references: 12636151
references: Acta Biomater. 2010 Dec;6(12):4650-6
references: 20643229
references: Acta Biomater. 2012 Feb;8(2):904-15
references: 22023752
references: Acta Biomater. 2010 Jul;6(7):2816-25
references: 20056173
references: J Mater Sci Mater Med. 2004 Apr;15(4):311-4
references: 15332591
references: Biomaterials. 2004 Sep;25(20):5013-21
references: 15109863
references: J Antimicrob Chemother. 2009 Feb;63(2):309-16
references: 19098295
references: Acta Biomater. 2007 Sep;3(5):817-27
references: 17478133
references: Acta Biomater. 2012 Aug;8(8):3144-52
references: 22487928
references: J Pharmacol Exp Ther. 2005 Feb;312(2):417-23
references: 15528454
references: Microbiology. 2002 Apr;148(Pt 4):1119-27
references: 11932456
references: Int J Nanomedicine. 2012;7:5641-52
references: 23152682
references: Chem Rev. 2001 Jul;101(7):1869-79
references: 11710233
references: J Control Release. 2002 Dec 5;84(3):115-23
references: 12468215
references: Int J Nanomedicine. 2011;6:581-90
references: 21674015
references: Appl Environ Microbiol. 2008 Nov;74(21):6639-48
references: 18791004
references: J Biomed Mater Res B Appl Biomater. 2010 Feb;92(2):298-306
references: 19904739
references: N Engl J Med. 2004 Oct 14;351(16):1645-54
references: 15483283
references: J Biomed Mater Res B Appl Biomater. 2009 Oct;91(1):470-80
references: 19637369
references: Acta Biomater. 2009 Nov;5(9):3573-80
references: 19454327
references: Bioresour Technol. 2010 Nov;101(22):8493-501
references: 20630747
references: Biomaterials. 2013 Aug;34(24):5969-77
references: 23680363
references: J Mech Behav Biomed Mater. 2008 Jan;1(1):30-42
references: 19627769
references: J Biomed Mater Res A. 2010 Feb;92(2):432-40
references: 19191311
references: Biomaterials. 2010 Sep;31(27):7196-204
references: 20573394
references: J Control Release. 2001 Jun 15;73(2-3):303-13
references: 11516507
references: Int J Nanomedicine. 2012;7:221-34
references: 22275837
references: Biomaterials. 2011 Aug;32(24):5706-16
references: 21565401
references: Antimicrob Agents Chemother. 1998 Nov;42(11):3002-5
references: 9797241