title: A novel artificial nerve graft for repairing long-distance sciatic nerve defects: a self-assembling peptide nanofiber scaffold-containing poly(lactic-co-glycolic acid) conduit.
abstract: In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold (SAPNS)-containing poly(lactic-co-glycolic acid) (PLGA) conduit (SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Xianghai
authors: X
authors: Wang
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Mengjie
authors: M
authors: Pan
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Jinkun
authors: J
authors: Wen
authors: Department of Histology and Embryology, Xiangnan University, Chenzhou, Hunan Province, China.
authors: Yinjuan
authors: Y
authors: Tang
authors: Department of Neurology, Vanderbilt University, Nashville, TN, USA.
authors: Audra D
authors: AD
authors: Hamilton
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Yuanyuan
authors: Y
authors: Li
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Changhui
authors: C
authors: Qian
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Zhongying
authors: Z
authors: Liu
authors: Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Guangzhou, Guangdong Province, China ; GHM Institute of CNS Regeneration, Jinan University, Guangzhou, Guangdong Province, China.
authors: Wutian
authors: W
authors: Wu
authors: Department of Histology and Embryology, Southern Medical University, Guangzhou, Guangdong Province, China ; Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou, Guangdong Province, China ; Institute of Bone Biology, Academy of Orthopedics, Guangzhou, Guangdong Province, China.
authors: Jiasong
authors: J
authors: Guo
keywords: NSFC grants; artificial nerve graft; axon; myelin; nanofiber scaffold; nerve regeneration; neural regeneration; neuromuscular junction; peripheral nerve defect; poly(lactic-co-glycolic acid); remyelination; self-assembling peptide
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