title: Molecular identification and classification of Trichophyton mentagrophytes complex strains isolated from humans and selected animal species.
abstract: Species differentiation within Trichophyton mentagrophytes complex group currently poses a major diagnostic challenge, with molecular methods increasingly supplementing classical identification based on the morphological and physiological properties of the fungi. Diagnostic and epidemiological research aimed at determining the source and means of transmission of dermatophytoses in both humans and animals requires not only species differentiation of isolates but also differentiation within species. The study was conducted on 24 isolates originating in humans and various animal species with clinical symptoms of dermatophytosis. The analysis included phenotypical identification methods and molecular methods: internal transcribed spacer sequencing and ITS-restriction fragment length polymorphism (RFLP) with multi-enzyme restriction. ITS sequence analysis identified the isolates to species - Trichophyton interdigitale, Arthroderma benhamiae and A. vanbreuseghemii, and ITS-RFLP detected six different genotypes. Genotypes I, II and III characterised strains belonging to A. benhamiae, genotype IV characterised the A. vanbreuseghemii strain, and genotypes V and VI occurred only within the species T. interdigitale. Strains isolated from guinea pigs were dominant within genotype I, while genotype II was found mainly in strains from foxes. Multi-enzyme restriction analysis of this region enables intraspecific differentiation, which may be useful in epidemiological research, particularly in determining the source of infections.
authors: Sub-Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Institute of Biological Bases of Animal Diseases, University of Life Sciences, Lublin, Poland.
authors: Grażyna
authors: G
authors: Ziółkowska
authors: Aneta
authors: A
authors: Nowakiewicz
authors: Sebastian
authors: S
authors: Gnat
authors: Aleksandra
authors: A
authors: Trościańczyk
authors: Przemysław
authors: P
authors: Zięba
authors: Barbara Majer
authors: BM
authors: Dziedzic
mesh_terms: D000818:Animals; D003883:Arthrodermataceae / Q000145:classification* / Q000235:genetics; D002415:Cats; D002417:Cattle; D002682:Chinchilla; D004271:DNA, Fungal / Q000235:genetics*; D021901:DNA, Intergenic; D005589:Foxes; D005838:Genotype; D006168:Guinea Pigs; D006801:Humans; D008907:Mink; D010641:Phenotype; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits; D017422:Sequence Analysis, DNA; D014005:Tinea / Q000382:microbiology* / Q000662:veterinary; D014249:Trichophyton / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic
keywords: Trichophyton mentagrophytes complex; intergenic transcribed spacer; restriction analysis; sequence analysis