title: Molecular and serological findings in suspected patients with Crimean-Congo hemorrhagic fever virus in Iran.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is an arthropod-borne disease of humans associated with a severe clinical picture, including hemorrhagic syndrome and a high mortality rate. CCHF virus is widely distributed throughout large areas of the world. To characterize the serological status in CCHF patients, paired clinical samples were collected from suspected CCHF patients and analyzed by microbiological and other laboratory analyses with the aim of: determining the presence of neutralizing antibodies against CCHF virus; investigating the cross-reactivity of these neutralizing antibodies against virus isolated from the same outbreak and against other available laboratory strain; and studying the relationship between the isolated virus with other virus by whole genome sequencing. Patients at Boo-Ali Hospital, Zahedan, Iran, with clinical symptoms ranging from mild to severe hemorrhagic fever were included in the study. Two serum samples were taken from each patient, the first as soon as the patient matched the criteria for CCHF notification and the second when the patient was discharged from hospital (2 weeks later). Commercial and in-house assays revealed a positive IgM signal in acute serum samples from six patients. A novel finding was that CCHF patients develop neutralizing antibodies soon after infection. Interestingly these antibodies were able to neutralize other CCHF virus strains too. The complete sequence of the Zahedan 2007 isolate, including the hitherto unknown first L-segment sequence, was identified using an original clinical sample from one patient with confirmed CCHF infection.
authors: Public Health Agency of Sweden, Stockholm, Sweden; Karolinska Institute, Stockholm, Sweden.
authors: Helen
authors: H
authors: Karlberg
authors: Batool
authors: B
authors: Sharifi-Mood
authors: Mehrdad
authors: M
authors: Mousavi-Jazi
authors: Meik
authors: M
authors: Dilcher
authors: Gunnel
authors: G
authors: Lindegren
authors: Masoud
authors: M
authors: Mardani
authors: Sandor
authors: S
authors: Bereskly
authors: Manfred
authors: M
authors: Weidmann
authors: Ali
authors: A
authors: Mirazimi
mesh_terms: D000293:Adolescent; D000328:Adult; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D016000:Cluster Analysis; D003429:Cross Reactions; D004196:Disease Outbreaks; D005260:Female; D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology* / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007075:Immunoglobulin M
keywords: CCHF; diagnostic; neutralization assay; serology