title: Evaluation of a topical herbal patch for soft tissue wound healing: an animal study.
abstract: OBJECTIVE
  This study evaluated the effects of a topical herbal patch (PerioPatch®) for gingival wound healing in a rat model.
  
  
  METHODS
  A mid-crestal incision was performed on each side of the edentulous anterior maxilla in 48, 6-month-old, Wistar rats. Full-thickness flaps were raised, repositioned and sutured. Four experimental groups were established: herbal patch, placebo patch, no patch and no patch and no surgery. Patches were placed immediately after surgery and replaced every 12 h for the following 3 days. Half of the animals were killed after 5 and the remaining ones after 12 days. Tissue blocks were retrieved and processed for histological and immunohistochemical evaluation. Epithelial gap, collagen contents, amount of macrophages, cellular proliferation and vascular contents were evaluated in the central incision area. Statistical analysis consisted of two-way anova.
  
  
  RESULTS
  The herbal patch group presented the smallest epithelial gap at 12 days, the highest collagen content both at 5 and 12 days, a larger number of proliferating cells at day 5 and more numerous blood vessels at day 12. Macrophage number was similar in all groups.
  
  
  CONCLUSIONS
  Herbal patch improved wound healing in this animal model.
authors: Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Liat
authors: L
authors: Chaushu
authors: Miron
authors: M
authors: Weinreb
authors: Ilan
authors: I
authors: Beitlitum
authors: Ofer
authors: O
authors: Moses
authors: Carlos E
authors: CE
authors: Nemcovsky
mesh_terms: D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D028041:Centella; D003094:Collagen / Q000032:analysis; D004353:Drug Evaluation, Preclinical; D020900:Echinacea; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005881:Gingiva / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D007575:Jaw, Edentulous / Q000601:surgery; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008437:Maxilla / Q000601:surgery; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D023421:Models, Animal; D008517:Phytotherapy / Q000379:methods*; D010919:Placebos; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000187:drug effects; D027924:Sambucus nigra; D013524:Surgical Flaps / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D014315:Triterpenes / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects
chemical_list: C526146:Centella asiatica extract; D010919:Placebos; D010936:Plant Extracts; D014315:Triterpenes; D003094:Collagen
keywords: animal study; collagen; epithelium; herbal; histology; immunohistochemistry; inflammation; topical; wound healing
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