title: An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells.
abstract: Hydrogen sulfide (H2S) has recently been identified as the third gaseous signaling molecule that is involved in regulating many important cellular processes. We report herein a novel fluorescent probe for detecting H2S based on iminocoumarin benzothiazole scaffold. The probe displayed high sensitivity and around 80-fold increment in fluorescence signal after reacting with H2S under physiological condition. The fluorescent intensity of the probe was linearly related to H2S concentration in the range of 0-100 μM with a detection limit of 0.15 μM (3σ/slope). The probe also showed excellent selectivity towards H2S over other biologically relevant species, including ROS, RSS and RNS. Its selectivity for H2S is 32 folds higher than other reactive sulfur species. Furthermore, the probe has been applied for imaging H2S in living cells. Cell imaging experiments demonstrated that the probe is cell-permeable and can be used to monitor the alteration of H2S concentrations in living cells. We envisage that this probe can provide useful tools to further elucidate the biological roles of H2S.
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Huatang
authors: H
authors: Zhang
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Yusheng
authors: Y
authors: Xie
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Ping
authors: P
authors: Wang
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Ganchao
authors: G
authors: Chen
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Ruochuan
authors: R
authors: Liu
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Yun-Wah
authors: YW
authors: Lam
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Key Lab of Nuclear Radiation and Nuclear Energy Technology, Center for Multidisciplinary Research, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China.
authors: Yi
authors: Y
authors: Hu
authors: Insitute of Bioengineering, Zhejiang University of Technology, Chaowang Road 18, Hangzhou 310014, China. Electronic address: zhuq@zjut.edu.cn.
authors: Qing
authors: Q
authors: Zhu
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address: hongysun@cityu.edu.hk.
authors: Hongyan
authors: H
authors: Sun
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D061848:Optical Imaging; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D052160:Benzothiazoles; D003374:Coumarins; D005456:Fluorescent Dyes; C005465:benzothiazole; D006862:Hydrogen Sulfide
keywords: Cell imaging; Fluorescent probe; Hydrogen sulfide; Iminocoumarin benzothiazole