title: Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?
abstract: OBJECTIVE
  To evaluate the tumor-inducing ability of a few leukemic cells xenotransplanted inside an artificial ovary.
  
  
  METHODS
  Ten and 100 BV-173 leukemic cells were embedded in a fibrin matrix along with 50,000 human ovarian stromal cells, and grafted to the peritoneal bursa of 5 and 5 SCID mice respectively. Four mice grafted with 3x10(6) leukemic cells in fibrin served as positive controls. At 20 weeks post-transplantation, the grafts, liver, spleen, blood and bone marrow were analyzed for the presence of leukemia by anti-CD79α IHC, flow cytometry (FC) and PCR.
  
  
  RESULTS
  All mice grafted with 3x10(6) cells developed peritoneal masses 4 weeks after xenotransplantation, and systemic disease was confirmed by IHC, PCR and FC. Among mice grafted with 10 or 100 leukemic cells, none showed any sign of leukemia after 20 weeks, and IHC, FC and PCR on the different recovered tissues all proved negative.
  
  
  CONCLUSIONS
  This study investigates the tumor-inducing potential of a few leukemic cells grafted inside an artificial ovary. Transplantation of 100 leukemic cells appears to be insufficient to induce leukemia after 20 weeks. These results in an immunodeficient xenografting model are quite reassuring. However, for clinical application, follicle suspensions must be purged of leukemic cells before grafting, as even the slightest risk should be avoided.
authors: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
authors: Michelle
authors: M
authors: Soares
authors: Pascale
authors: P
authors: Saussoy
authors: Karima
authors: K
authors: Sahrari
authors: Christiani A
authors: CA
authors: Amorim
authors: Jacques
authors: J
authors: Donnez
authors: Marie-Madeleine
authors: MM
authors: Dolmans
mesh_terms: D000818:Animals; D001187:Artificial Organs; D005260:Female; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D008954:Models, Biological; D009368:Neoplasm Transplantation / Q000379:methods*; D010053:Ovary; D017154:Stromal Cells / Q000637:transplantation; D014183:Transplantation, Heterologous / Q000379:methods
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