title: Oxidative stress markers are increased in patients with mastocytosis.
abstract: BACKGROUND
  Mastocytosis is characterized by clonal proliferation of mast cells limited to the skin (cutaneous mastocytosis: CM and mastocytosis in the skin: MIS) and/or involving internal organs (systemic mastocytosis: SM). Oxidative stress occurring in various inflammatory and neoplastic disorders causes molecular damage with the production of advanced oxidation protein products (AOPPs) and advanced glycation end products (AGEs). We evaluated these markers of oxidative stress in patients with CM/MIS and SM and correlated their levels with the presence of symptoms related to mast cell activation.
  
  
  METHODS
  Serum levels of AOPPs and AGEs in 34 patients with mastocytosis (23 CM/MIS and 11 SM) and 27 healthy controls were measured by spectrofluorimetric and spectrophotometric methods. Serum tryptase levels were measured by immunofluorescence.
  
  
  RESULTS
  Serum AOPPs, but not AGEs, were significantly higher in patients with mastocytosis as compared to healthy controls. While serum tryptase levels were higher in patients with SM as compared to those with CM/MIS, there was no difference in AOPP and AGE concentrations between these two groups of patients. Patients with recurrent mediator-related symptoms had lower AOPPs and AGEs as compared to patients without symptoms. AOPPs and AGEs were inversely correlated with the severity of symptoms, and in patients with symptoms, AOPPs correlated with tryptase levels.
  
  
  CONCLUSIONS
  Our data show that mastocytosis is associated with a state of increased oxidative stress that, in patients with mediator-related symptoms, correlates with mast cell burden as assessed by tryptase. Patients with symptoms presumably have an adaptive response resulting in lower blood levels of AOPPs and AGEs.
authors: Operative Unit of Allergy and Clinical Immunology, Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy; Institute of Clinical Physiology, IFC CNR, Messina Unit, Messina, Italy.
authors: S
authors: S
authors: Gangemi
authors: P L
authors: PL
authors: Minciullo
authors: D
authors: D
authors: Magliacane
authors: S
authors: S
authors: Saitta
authors: S
authors: S
authors: Loffredo
authors: A
authors: A
authors: Saija
authors: M
authors: M
authors: Cristani
authors: G
authors: G
authors: Marone
authors: M
authors: M
authors: Triggiani
mesh_terms: D000293:Adolescent; D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood; D006801:Humans; D007223:Infant; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008415:Mastocytosis / Q000097:blood* / Q000175:diagnosis*; D008875:Middle Aged; D018384:Oxidative Stress; D053802:Tryptases / Q000097:blood; D055815:Young Adult
chemical_list: D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D017127:Glycation End Products, Advanced; D053802:Tryptases
keywords: advanced glycation end product; advanced oxidation protein product; mast cell; mastocytosis; oxidative stress