title: Visualizing hypothalamic network dynamics for appetitive and consummatory behaviors.
abstract: Optimally orchestrating complex behavioral states, such as the pursuit and consumption of food, is critical for an organism's survival. The lateral hypothalamus (LH) is a neuroanatomical region essential for appetitive and consummatory behaviors, but whether individual neurons within the LH differentially contribute to these interconnected processes is unknown. Here, we show that selective optogenetic stimulation of a molecularly defined subset of LH GABAergic (Vgat-expressing) neurons enhances both appetitive and consummatory behaviors, whereas genetic ablation of these neurons reduced these phenotypes. Furthermore, this targeted LH subpopulation is distinct from cells containing the feeding-related neuropeptides, melanin-concentrating hormone (MCH), and orexin (Orx). Employing in vivo calcium imaging in freely behaving mice to record activity dynamics from hundreds of cells, we identified individual LH GABAergic neurons that preferentially encode aspects of either appetitive or consummatory behaviors, but rarely both. These tightly regulated, yet highly intertwined, behavioral processes are thus dissociable at the cellular level.
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Joshua H
authors: JH
authors: Jennings
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Randall L
authors: RL
authors: Ung
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Shanna L
authors: SL
authors: Resendez
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Alice M
authors: AM
authors: Stamatakis
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Johnathon G
authors: JG
authors: Taylor
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Jonathan
authors: J
authors: Huang
authors: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Katie
authors: K
authors: Veleta
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Pranish A
authors: PA
authors: Kantak
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Megumi
authors: M
authors: Aita
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Kelson
authors: K
authors: Shilling-Scrivo
authors: Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
authors: Charu
authors: C
authors: Ramakrishnan
authors: Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
authors: Karl
authors: K
authors: Deisseroth
authors: Inscopix Inc., Palo Alto, CA 94303, USA.
authors: Stephani
authors: S
authors: Otte
authors: Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA. Electronic address: gstuber@med.unc.edu.
authors: Garret D
authors: GD
authors: Stuber
mesh_terms: D000818:Animals; D001070:Appetitive Behavior; D003259:Consummatory Behavior; D007028:Hypothalamic Hormones / Q000378:metabolism; D007031:Hypothalamus / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D051379:Mice; D009042:Motivation; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D000068797:Orexins; D010907:Pituitary Hormones / Q000378:metabolism; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D007028:Hypothalamic Hormones; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009479:Neuropeptides; D000068797:Orexins; D010907:Pituitary Hormones; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; D005680:gamma-Aminobutyric Acid; C019463:melanin-concentrating hormone
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