title: Biodistribution of a Promising Probiotic, Bifidobacterium longum subsp. longum Strain BBMN68, in the Rat Gut.
abstract: Bifidobacterium longum subsp. longum BBMN68, isolated from centenarians in Guangxi, China, has been proved to be a promising probiotic strain for its health benefits. In this study, the biodistribution of this strain in the rat gut was first investigated using the quantitative realtime PCR assay and propidium monoazide. Strain-specific primers were originally designed based on the BBMN68 genome sequence. Healthy rats were orally inoculated with either a single dose of BBMN68 (10(10) colony-forming units/kg), or with one dose per day for 7 days and bacterial concentrations were analyzed in detail from the intestinal contents and feces of four different gut locations, including stomach, small intestine, colon, and rectum. Results indicated that strain BBMN68 could overcome the rigors of passage through the upper gastrointestinal tract and transiently accumulate in the colon, even though survival in the stomach and small intestine was not high. A good level of BBMN8 could stay in vivo for 72 h following a 7-day oral administration, and a daily administration is suggested for a considerable and continuous population of BBMN68 to be maintained in the host intestine.
authors: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 10083, P.R. China.|Beijing Laboratory for Food Quality and Safety, Beijing 102206, P.R. China.
authors: Yang
authors: Y
authors: Lv
authors: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 10083, P.R. China.
authors: Xuewei
authors: X
authors: Qiao
authors: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 10083, P.R. China.|Beijing Higher Institution Engineering Research Center of Animal Product, Beijing 100083, P.R. China.
authors: Liang
authors: L
authors: Zhao
authors: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 10083, P.R. China.|Beijing Laboratory for Food Quality and Safety, Beijing 102206, P.R. China.
authors: Fazheng
authors: F
authors: Ren
mesh_terms: D000284:Administration, Oral; D000818:Animals; D058491:Bacterial Load; D001644:Bifidobacterium / Q000302:isolation & purification*; D001682:Biological Availability; D002681:China; D041981:Gastrointestinal Tract / Q000382:microbiology*; D050296:Microbial Viability; D019936:Probiotics / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction