title: Glycolytic inhibitors 2-deoxyglucose and 3-bromopyruvate synergize with photodynamic therapy respectively to inhibit cell migration.
abstract: Most cancer cells have the specially increased glycolytic phenotype, which makes this pathway become an attractive therapeutic target. Although glycolytic inhibitor 2-deoxyglucose (2-DG) has been demonstrated to potentiate the cytotoxicity of photodynamic therapy (PDT), the impacts on cell migration after the combined treatment has never been reported yet. The present study aimed to analyze the influence of glycolytic inhibitors 2-DG and 3-bromopyruvate (3-BP) combined with Ce6-PDT on cell motility of Triple Negative Breast Cancer MDA-MB-231 cells. As determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium-bromide-Tetraz-olium (MTT) assay, more decreased cell viability was observed in 2-DG + PDT and 3-BP + PDT groups when compared with either monotherapy. Under optimal conditions, synergistic potentiation on cell membrane destruction and the decline of cell adhesion and cells migratory ability were observed in both 2-DG + PDT and 3-BP + PDT by electron microscope observation (SEM), wound healing and trans-well assays. Besides, serious microfilament network collapses as well as impairment of matrix metalloproteinases-9 (MMP-9) were notably improved after the combined treatments by immunofluorescent staining. These results suggest that 2-DG and 3-BP can both significantly potentiated Ce6-PDT efficacy of cell migration inhibition.
authors: Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China.
authors: Xiaolan
authors: X
authors: Feng
authors: Pan
authors: P
authors: Wang
authors: Quanhong
authors: Q
authors: Liu
authors: Ting
authors: T
authors: Zhang
authors: Bingjie
authors: B
authors: Mai
authors: Xiaobing
authors: X
authors: Wang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003847:Deoxyglucose / Q000494:pharmacology*; D004357:Drug Synergism; D005455:Fluorescent Antibody Technique; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010778:Photochemotherapy / Q000379:methods*; D011773:Pyruvates / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy
chemical_list: D011773:Pyruvates; D013778:Tetrazolium Salts; D013844:Thiazoles; C017092:bromopyruvate; D003847:Deoxyglucose; C022616:thiazolyl blue
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