title: Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis.
abstract: Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.
authors: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
authors: Marcelo Andreetta
authors: MA
authors: Corral
authors: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, São Paulo, SP, Brazil.
authors: Fabiana Martins
authors: FM
authors: Paula
authors: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
authors: Maiara
authors: M
authors: Gottardi
authors: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, São Paulo, SP, Brazil.
authors: Dirce Mary Correia Lima
authors: DM
authors: Meisel
authors: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
authors: Pedro Paulo
authors: PP
authors: Chieffi
authors: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
authors: Ronaldo César Borges
authors: RC
authors: Gryschek
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008566:Membranes / Q000276:immunology; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G
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