title: Induced jasmonate signaling leads to contrasting effects on root damage and herbivore performance.
abstract: Induced defenses play a key role in plant resistance against leaf feeders. However, very little is known about the signals that are involved in defending plants against root feeders and how they are influenced by abiotic factors. We investigated these aspects for the interaction between rice (Oryza sativa) and two root-feeding insects: the generalist cucumber beetle (Diabrotica balteata) and the more specialized rice water weevil (Lissorhoptrus oryzophilus). Rice plants responded to root attack by increasing the production of jasmonic acid (JA) and abscisic acid, whereas in contrast to in herbivore-attacked leaves, salicylic acid and ethylene levels remained unchanged. The JA response was decoupled from flooding and remained constant over different soil moisture levels. Exogenous application of methyl JA to the roots markedly decreased the performance of both root herbivores, whereas abscisic acid and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid did not have any effect. JA-deficient antisense 13-lipoxygenase (asLOX) and mutant allene oxide cyclase hebiba plants lost more root biomass under attack from both root herbivores. Surprisingly, herbivore weight gain was decreased markedly in asLOX but not hebiba mutant plants, despite the higher root biomass removal. This effect was correlated with a herbivore-induced reduction of sucrose pools in asLOX roots. Taken together, our experiments show that jasmonates are induced signals that protect rice roots from herbivores under varying abiotic conditions and that boosting jasmonate responses can strongly enhance rice resistance against root pests. Furthermore, we show that a rice 13-lipoxygenase regulates root primary metabolites and specifically improves root herbivore growth.
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Jing
authors: J
authors: Lu
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Christelle Aurélie Maud
authors: CA
authors: Robert
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Michael
authors: M
authors: Riemann
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Marco
authors: M
authors: Cosme
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Laurent
authors: L
authors: Mène-Saffrané
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Josep
authors: J
authors: Massana
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Michael Joseph
authors: MJ
authors: Stout
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Yonggen
authors: Y
authors: Lou
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.).
authors: Jonathan
authors: J
authors: Gershenzon
authors: Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany (J.L., C.A.M.R., J.G., M.E.);Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Karlsruhe Institute of Technology, Botanical Institute-Molecular Cell Biology, 76131 Karlsruhe, Germany (M.R.);Functional Biodiversity, Dahlem Center of Plant Sciences, Freie Universität Berlin, 14195 Berlin, Germany (M.C.);Department of Plant Biology, University of Fribourg, 1700 Fribourg, Switzerland (L.M.-S., J.M.);Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 (M.J.S.); andInstitute of Insect Science, Zijingang Campus, Zhejiang University, Hangzhou 310058, China (Y.L.) matthias.erb@ips.unibe.ch.
authors: Matthias
authors: M
authors: Erb
mesh_terms: D000818:Animals; D018533:Biomass; D001517:Coleoptera / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D060434:Herbivory; D012275:Oryza / Q000378:metabolism* / Q000469:parasitology*; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013395:Sucrose / Q000378:metabolism; D014867:Water
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014867:Water; D013395:Sucrose; C011006:jasmonic acid
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