title: iTRAQ-based quantitative proteomic analysis of cervical cancer.
abstract: Cervical cancer is the seventh most common cancer overall and the third among females. To obtain systematic insight into the protein profile that participates in cervical tumor oncogenesis and improve the current target therapies, iTRAQ labeling and NanoLC-MS/MS analysis were utilized to detect differentially expressed proteins in cervical cancer. As a result, 3,647 proteins were identified, among which the expression levels of 294 proteins in cervical cancer samples were distinct from the paired non-tumor samples. Further validation of the differentially expressed proteins, including G6PD, ALDH3A1, STAT1 and HSPB1, was carried out via qRT-PCR, western blot analysis and tissue microarray. Functional analysis of one of the highly expressed proteins, G6PD, was performed using RNA interference. Attenuated G6PD expression reduced the capacity of HeLa cells to migrate and invade in vitro. Our investigation complemented the understanding of cervical cancer progression. Furthermore, the present study supports the notion that suppressing the expression of G6PD may be a promising strategy in developing novel cancer therapeutic drugs.
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Yibing
authors: Y
authors: Ding
authors: Department of Infectious Diseases, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Min
authors: M
authors: Yang
authors: Department of Infectious Diseases, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Sha
authors: S
authors: She
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Haiyan
authors: H
authors: Min
authors: Department of Infectious Diseases, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Xiaoming
authors: X
authors: Xv
authors: Department of Infectious Diseases, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Xiaoping
authors: X
authors: Ran
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Yongzheng
authors: Y
authors: Wu
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Wei
authors: W
authors: Wang
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Lei
authors: L
authors: Wang
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Long
authors: L
authors: Yi
authors: Department of Infectious Diseases, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Yixuan
authors: Y
authors: Yang
authors: Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.
authors: Qian
authors: Q
authors: Gao
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006360:Heat-Shock Proteins; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D018832:Molecular Chaperones; D040901:Proteomics / Q000379:methods*; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods; D046888:Tissue Array Analysis / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D005954:Glucosephosphate Dehydrogenase; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase