title: Calretinin immunoreactivity in the claustrum of the rat.
abstract: The claustrum is a telencephalic structure which consists of dorsal segment adjoining the insular cortex and a ventral segment termed also endopiriform nucleus (END). The dorsal segment (claustrum) is divided into a dorsal and ventral zone, while the END is parcellated into dorsal, ventral and intermediate END. The claustrum and the END consist of glutamatergic projection neurons and GABAergic local interneurons coexpressing calcium binding proteins. Among neurons expressing calcium binding proteins the calretinin (CR)-immunoreactive interneurons exert specific functions in neuronal circuits, including disinhibition of excitatory neurons. Previous anatomical data indicate extensive and reciprocally organized claustral projections with cerebral cortex. We asked if the distribution of cells immunoreactive for CR delineates anatomical or functional subdivisions in the claustrum and in the END. Both segments of the claustrum and all subdivisions of the END contained CR immunoreactive neurons with varying distribution. The ventral zone of the claustrum exhibited weak labeling with isolated cell bodies and thin fibers and is devoid of immunoreactive puncta. Within the medial margin of the intermediate END we noted a group of strongly positive neurons. Cells immunoreactive for CR in all subdivisions of the claustrum and END were bipolar, multipolar and oval with smooth, beaded aspiny dendrites. Small number of CR-immunoreactive neurons displayed thin dendrites which enter to adjoining structures. Penetration of dendrites was reciprocal. These results show an inhomogenity over the claustrum and the END in distribution and types of CR immunoreactive neurons. The distribution of the CR-immunoreactive neurons respects the anatomical but not functional zones of the claustral complex.
authors: Department of Anatomy, Charles University in Prague, 2nd Faculty of Medicine Prague, Czech Republic ; Department of Anatomy, Charles University in Prague, 1st Faculty of Medicine Prague, Czech Republic ; Department of Developmental Epileptology, Institute of Physiology, Academy of Sciences of the Czech Republic Prague, Czech Republic.
authors: Rastislav
authors: R
authors: Druga
authors: Department of Anatomy, Charles University in Prague, 2nd Faculty of Medicine Prague, Czech Republic.
authors: Martin
authors: M
authors: Salaj
authors: Department of Neurology, University of Regensburg Regensburg, Germany.
authors: Filip
authors: F
authors: Barinka
authors: Medimark Corporation Del Mar, CA, USA.
authors: Lawrence
authors: L
authors: Edelstein
authors: Department of Developmental Epileptology, Institute of Physiology, Academy of Sciences of the Czech Republic Prague, Czech Republic.
authors: Hana
authors: H
authors: Kubová
keywords: calcium-binding proteins; calretinin; claustrum; endopiriform nucleus; rat
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