title: Synergistic effects of atmospheric pressure plasma-emitted components on DNA oligomers: a Raman spectroscopic study.
abstract: Cold atmospheric-pressure plasmas have become of increasing importance in sterilization processes especially with the growing prevalence of multi-resistant bacteria. Albeit the potential for technological application is obvious, much less is known about the molecular mechanisms underlying bacterial inactivation. X-jet technology separates plasma-generated reactive particles and photons, thus allowing the investigation of their individual and joint effects on DNA. Raman spectroscopy shows that particles and photons cause different modifications in DNA single and double strands. The treatment with the combination of particles and photons does not only result in cumulative, but in synergistic effects. Profilometry confirms that etching is a minor contributor to the observed DNA damage in vitro. Schematics of DNA oligomer treatment with cold atmospheric-pressure plasma.
authors: Physical Chemistry II, Chemistry and Biochemistry, Ruhr University Bochum, Germany.
authors: Eugen
authors: E
authors: Edengeiser
authors: Biology of Microorganisms, Biology and Biotechnology, Ruhr University Bochum, Germany.
authors: Jan-Wilm
authors: JW
authors: Lackmann
authors: Physical Chemistry II, Chemistry and Biochemistry, Ruhr University Bochum, Germany.
authors: Erik
authors: E
authors: Bründermann
authors: Coupled Plasma-Solid State Systems, Physics and Astronomy, Ruhr University Bochum, Germany.
authors: Simon
authors: S
authors: Schneider
authors: Coupled Plasma-Solid State Systems, Physics and Astronomy, Ruhr University Bochum, Germany.
authors: Jan
authors: J
authors: Benedikt
authors: Biology of Microorganisms, Biology and Biotechnology, Ruhr University Bochum, Germany. julia.bandow@rub.de.
authors: Julia E
authors: JE
authors: Bandow
authors: Physical Chemistry II, Chemistry and Biochemistry, Ruhr University Bochum, Germany.
authors: Martina
authors: M
authors: Havenith
mesh_terms: D019540:Area Under Curve; D001274:Atmospheric Pressure; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004867:Equipment Design; D006371:Helium; D010100:Oxygen; D017785:Photons; D058626:Plasma Gases; D013059:Spectrum Analysis, Raman
chemical_list: D058626:Plasma Gases; D006371:Helium; D004247:DNA; D010100:Oxygen
keywords: DNA; Raman spectroscopy; atmospheric-pressure plasma; plasma medicine