title: Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development.
abstract: Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding tissue. We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the three-dimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire zebrafish embryos over several hours of development, as well as segmentation, tracking and automatic registration of individual organs.
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany Politecnico di Milano, Dipartimento di Fisica, Milano 20133, Italy.
authors: Andrea
authors: A
authors: Bassi
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
authors: Benjamin
authors: B
authors: Schmid
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany huisken@mpi-cbg.de.
authors: Jan
authors: J
authors: Huisken
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology; D008853:Microscopy / Q000379:methods*; D041622:Tomography, Optical / Q000379:methods*; D015027:Zebrafish / Q000196:embryology*
keywords: Fluorescence; Light sheet microscopy; Optical tomography; SPIM; Time-lapse imaging; Zebrafish
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