title: Combination of IgG N-glycomics and corresponding transcriptomics data to identify anti-TNF-α treatment responders in inflammatory diseases.
abstract: Prediction of responsiveness in biological therapies is an important and challenging issue in different diseases. Analyzing glycosylation pattern changes of key serum glycoproteins is one of the possible avenues to follow disease remission. The aim of this study was to investigate the changes of serum IgG glycoforms in Crohn's disease (CD) and rheumatoid arthritis patients in response to antitumor necrosis factor alpha (anti-TNF-α) treatment. IgG was isolated from patient serum samples using Protein A affinity pull-down, followed by the release of N-glycans with peptide-N-glycosidase F. The released glycans were fluorescently tagged with 8-aminopyrene-1,3,6-trisulfonate and analyzed by CGE with laser-induced fluorescent detection. Significant alterations were detected between responders and nonresponders in both disease groups. In CD patients, disease-specific alteration was found in response to anti-TNF-α therapy, which was also confirmed by transcriptomics data analysis of the corresponding glycosyltransferases and glycosidases.
authors: Horváth Laboratory of Bioseparation Sciences, School of Medicine, MMKK, University of Debrecen, Debrecen, Hungary.
authors: Csaba
authors: C
authors: Váradi
authors: EGIS Pharmaceuticals, Budapest, Hungary.
authors: Zsolt
authors: Z
authors: Holló
authors: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors: Szilárd
authors: S
authors: Póliska
authors: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors: László
authors: L
authors: Nagy
authors: Department of Rheumatology, School of Medicine, University of Debrecen, Debrecen, Hungary.
authors: Zoltán
authors: Z
authors: Szekanecz
authors: Department of Rheumatology, School of Medicine, University of Debrecen, Debrecen, Hungary.
authors: Andrea
authors: A
authors: Váncsa
authors: Department of Internal Medicine, University of Debrecen, Debrecen, Hungary.
authors: Károly
authors: K
authors: Palatka
authors: Horváth Laboratory of Bioseparation Sciences, School of Medicine, MMKK, University of Debrecen, Debrecen, Hungary.|MTA-PE Translational Glycomics Research Group, MUKKI, University of Pannonia, Veszprém, Hungary.
authors: András
authors: A
authors: Guttman
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology; D003424:Crohn Disease / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D054794:Glycomics; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008297:Male; D059467:Transcriptome; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha
keywords: Capillary electrophoresis; Glycosylation; IgG