title: Comprehensive identification of high-frequency and combination MHC-DMA and -DMB alleles in a cohort of Chinese rhesus macaques and cynomolgus macaques of Vietnamese origin.
abstract: Rhesus and cynomolgus macaques are currently used as ideal animal models of immune response. Major histocompatibility complex (MHC) molecules play important roles in the susceptibility and/or resistance to many diseases. In this study, MHC-DMA and -DMB were first characterized by sequencing and cloning in 28 unrelated cynomolgus macaques from Vietnam and 34 unrelated Chinese rhesus macaques. A total of 23 novel alleles, including six high frequency alleles, were identified in this study. Our results showed that the alleles with the highest phenotypic frequencies were Mafa-DMA(∗)02:04:03 (57.1%), Mafa-DMB(∗)03:01:02 (76.9%), Mamu-DMA(∗)02:01:04 (88.2%), and Mamu-DMB(∗)03:02:02 (85%), respectively, indicating that distribution and frequencies of alleles had a few differences between Chinese rhesus macaques and cynomolgus macaques from Vietnam. Interestingly, compared to the cynomolgus macaques, we found that the combination of Mamu-DMA(∗)02:01:04-DMB(∗)03:02:02 was detected in 27 (79.4%) of 34 monkeys, suggesting that the combination of the MHC-DMA and -DMB alleles was probably a characteristic feature of the Chinese rhesus macaques. Our results will greatly increase the value of the two species as models for biomedical research.
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Beilei
authors: B
authors: Liu
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yabin
authors: Y
authors: Jin
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yueer
authors: Y
authors: Lu
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yiran
authors: Y
authors: Wu
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yanxia
authors: Y
authors: Xue
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yonghui
authors: Y
authors: Zhang
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Yang
authors: Y
authors: Liu
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors: Min
authors: M
authors: Zhuo
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: fling@scut.edu.cn.
authors: Fei
authors: F
authors: Ling
mesh_terms: D000483:Alleles; D000818:Animals; D002681:China; D006681:HLA-D Antigens / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D007109:Immunity / Q000235:genetics; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008285:Major Histocompatibility Complex / Q000235:genetics*; D023421:Models, Animal; D011110:Polymorphism, Genetic; D014744:Vietnam
chemical_list: D006681:HLA-D Antigens; C084245:HLA-DM antigens
keywords: High-frequency; Macaca fascicularis; Macaca mulatta; Major histocompatibility complex class II