title: Protease-activated ratiometric fluorescent probe for pH mapping of malignant tumors.
abstract: A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was covalently attached to the antitumor antibody. The optical response of the probe to activated MMP-9 and gastric cell line SGC7901 tumor cells was investigated, followed by in vivo tumor imaging. Based on the ratiometric pH response to the tumor microenvironment, the resulting probe was successfully used to image the pH of subcutaneous tumor xenografts.
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Yi
authors: Y
authors: Hou
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Jin
authors: J
authors: Zhou
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.|‡College of Chemistry, Jilin University, Changchun 130012, China.
authors: Zhenyu
authors: Z
authors: Gao
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Xiaoyu
authors: X
authors: Sun
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Chunyan
authors: C
authors: Liu
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Dihua
authors: D
authors: Shangguan
authors: ‡College of Chemistry, Jilin University, Changchun 130012, China.
authors: Wensheng
authors: W
authors: Yang
authors: †Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors: Mingyuan
authors: M
authors: Gao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology; D059016:Tumor Microenvironment
chemical_list: D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D020780:Matrix Metalloproteinase 9
keywords: Fe3O4 nanocrystals; pH mapping; protease-activated; ratiometric fluorescence; smart probe