title: Polyelectrolyte-based electrochemiluminescence enhancement for Ru(bpy)₃²⁺ loaded by SiO₂ nanoparticle carrier and its high sensitive immunoassay.
abstract: In this paper the strong electrochemiluminescence (ECL) nanoparticles have been prepared based on the anionic polyelectrolyte sodium polyacrylate (PAA)-ECL enhancement for Ru(bpy)3(2+), which were loaded by the carrier of SiO2 nanoparticle. There were two kinds of Ru(bpy)3(2+) for the as-prepared nanoparticles, the doped one and the exchanged one. The former was loaded inside the ECL nanoparticles by doping, in a form of ion-pair macromolecules PAA-Ru(bpy)3(2+). The corresponding ECL was enhanced about 2 times owing to the doping increase of Ru(bpy)3(2+). The latter was loaded on the PAA-doped Nafion membrane by ion exchange. The corresponding ECL was enhanced about 3 times owing to the ion-exchanging increase of Ru(bpy)3(2+). At the same time, ECL intensity of the doped-inside Ru(bpy)3(2+) was further enhanced 13 times because polyelectrolyte PAA in the doped membrane could obviously enhance electron transfer between the doped Ru(bpy)3(2+) and the working electrode. Furthermore, based on hydrophobic regions of the doped membrane antibody labeling could be easily realized by the as-prepared nanoparticles and then a high sensitive ECL immunoassay for HBsAg was developed. The linear range was between 1.0 and 100 pg mL(-1) (R(2)=0.9912). The detection limit could be as low as 0.11 pg mL(-1) (signal-to-noise ratio=3).
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors: Zhi-Li
authors: ZL
authors: Ge
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors: Tian-Mei
authors: TM
authors: Song
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors: Zhe
authors: Z
authors: Chen
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China; College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, PR China.
authors: Wu-Run
authors: WR
authors: Guo
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China. Electronic address: hpxie@suda.edu.cn.
authors: Hong-Ping
authors: HP
authors: Xie
authors: College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China. Electronic address: xielian@suda.edu.cn.
authors: Lian
authors: L
authors: Xie
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D056831:Coordination Complexes; D055664:Electrochemical Techniques; D004573:Electrolytes / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D000180:Acrylic Resins; D056831:Coordination Complexes; D004573:Electrolytes; D006514:Hepatitis B Surface Antigens; D012822:Silicon Dioxide; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C006903:carbopol 940
keywords: Electrochemiluminescence enhancement; High sensitive immunoassay; Ion-pair macromolecule doping; Polyelectrolyte-doped membrane; Strong electrochemiluminescence nanoparticles