title: Aptamer decorated hyaluronan/chitosan nanoparticles for targeted delivery of 5-fluorouracil to MUC1 overexpressing adenocarcinomas.
abstract: An aptamer (Apt) conjugated hyaluronan/chitosan nanoparticles (HACSNPs) were prepared as carrier for targeted delivery of 5-fluorouracil (5FU) to mucin1 (MUC1) overexpressing colorectal adenocarcinomas. Nanoparticles had about 181 nm size, encapsulation efficiency of 45.5 ± 2.8 and acceptable stability. Conjugation of MUC1-binding Apt to the surface of the nanoparticles was confirmed by gel electrophoresis. Toxicity and cellular uptake of nanoparticles were investigated by in vitro cytotoxicity assays and confocal scanning microscopy in (MUC1(+)) human adenocarcinoma and (MUC1(-)) Chinese hamster ovary cells. Toxicity of nanoparticles were significantly higher in comparison with free drug in both cell lines while this rising was more efficient for nanoparticles decorated with Apt in MUC1(+) cell line. The same result was observed in the cellular uptake study. It could be concluded that the present system has the potential to be considered in treatment of MUC1(+) colorectal adenocarcinomas.
authors: Dept. of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14174, Iran.
authors: Zahra
authors: Z
authors: Ghasemi
authors: Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Rassoul
authors: R
authors: Dinarvand
authors: Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Fatemeh
authors: F
authors: Mottaghitalab
authors: Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mehdi
authors: M
authors: Esfandyari-Manesh
authors: Dept. of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14174, Iran.
authors: Elmira
authors: E
authors: Sayari
authors: Dept. of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14174, Iran. Electronic address: atyabifa@tums.ac.ir.
authors: Fatemeh
authors: F
authors: Atyabi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000494:pharmacology; D016466:CHO Cells; D048271:Chitosan / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005472:Fluorouracil / Q000737:chemistry* / Q000627:therapeutic use; D019073:HT29 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; C060192:MUC1 protein, human; D018396:Mucin-1; D006820:Hyaluronic Acid; D048271:Chitosan; D005472:Fluorouracil
keywords: 5-Fluorouracil; Chitosan; Hyaluronan; MUC1-binding aptamer