title: Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice.
abstract: BACKGROUND
  Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model.
  
  
  METHODS
  BALB/c mice were immunized three times either autoclaved L. major promastigotes' antigens alone or in combination with the adjuvant alum, naltrexone or the alum-naltrexone mixture. Both humoral and cellular immune responses were assessed two weeks after the last immunization and compared with control mice.
  
  
  RESULTS
  The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major. There was no significant difference in survival following challenge among groups.
  
  
  CONCLUSIONS
  Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.
authors: Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors: Habib
authors: H
authors: Mohammadzadeh Hajipirloo
authors: Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors: Arezoo
authors: A
authors: Bozorgomd
authors: Dept. of Microbiology, Immunology and Genetics, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors: Shahram
authors: S
authors: Shahabi
authors: Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors: Khosrow
authors: K
authors: Hazrati Tappeh
authors: Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Seyed Ahmad
authors: SA
authors: Karamati
keywords: Adjuvant; Alum; Antigen; BALB/c; Leishmania major; Naltrexone