title: Creeping proteins in microporous structures: polymer brush-assisted fabrication of 3D gradients for tissue engineering.
abstract: Coupling of rapid prototyping techniques and surface-confined polymerizations allows the fabrication of 3D multidirectional gradients of biomolecules within microporous scaffolds. The compositional gradients can be tailored by polymer-brush-assisted diffusion of protein solutions. This technique allows spatial control over stem cells manipulation within 3D environments.
authors: Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
authors: Michel Klein
authors: MK
authors: Gunnewiek
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
authors: Andrea
authors: A
authors: Di Luca
authors: Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
authors: Hermannes Z
authors: HZ
authors: Bollemaat
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.|Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, P.O. Box 616, 6200, MD, Maastricht, The Netherlands.
authors: Clemens A
authors: CA
authors: van Blitterswijk
authors: Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
authors: G Julius
authors: GJ
authors: Vancso
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.|Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, P.O. Box 616, 6200, MD, Maastricht, The Netherlands.
authors: Lorenzo
authors: L
authors: Moroni
authors: Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.|Laboratory for Surface Science and Technology (LSST), Department of Materials, ETH Zürich, Vladimir-Prelog-Weg 5, CH-8093, Zürich, Switzerland.
authors: Edmondo M
authors: EM
authors: Benetti
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D018914:Cells, Immobilized; D006801:Humans; D059630:Mesenchymal Stem Cells; D008962:Models, Theoretical; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols
keywords: 3D scaffolds; poly(ε-caprolactone); polymer brushes; protein gradients; wetting