title: [Effect of umbilical cord blood mesenchymal stem cells on peripheral blood lymphocyte subsets].
abstract: OBJECTIVE
  This study was aimed to investigate the effect of mesenchymal stem cells (MSCs) on subsets and cytokine secretion of T lymphocytes.
  
  
  METHODS
  Umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) were isolated by density gradient and were cultared by amplifying culture. The subsets and cytokine secretion of T lymphocytes were detected by flow cytomety after being co-cultured with UCBMSC.
  
  
  RESULTS
  The proliferation of lymphocytes was inhibited. CD4(+)T cell subsets were increased, CD8(+)T cell subsets decreased when co-cultured with UCBMSC; Th1 and Tc1 level significantly reduced, while Th2 and Tc2 level slightly increased.
  
  
  CONCLUSIONS
  The UCBMSC can inhibit the proliferation of lymphocytes, especially CD8(+)T cell subsets. In addition, UCBMSCs can reduce Th1 and Tc1 cells, and increase Th2 and Tc2 cells. UCBMSC may have the clinical application potential for preventing and remedying GVHD.
authors: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors: Zhi-Juan
authors: ZJ
authors: Xu
authors: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China.
authors: Li-Xia
authors: LX
authors: Sheng
authors: Ningbo Municipal First Hospital Affiliated to Ningbo University Medical School, Ningbo 315000, Zhejiang Province, China. E-mail: ouyangguifang@ medmail.com.cn.
authors: Gui-Fang
authors: GF
authors: Ouyang
mesh_terms: D018920:Coculture Techniques; D005312:Fetal Blood; D006801:Humans; D018655:Lymphocyte Count; D059630:Mesenchymal Stem Cells; D013234:Stem Cells; D016176:T-Lymphocyte Subsets