title: Antiproliferative activity of Humulus lupulus extracts on human hepatoma (Hep3B), colon (HT-29) cancer cells and proteases, tyrosinase, β-lactamase enzyme inhibition studies.
abstract: The aims of this study were to examine the antiproliferation of Humulus lupulus extracts on human hepatoma carcinoma (Hep3B) and human colon carcinoma (HT-29) cell lines along with enzyme inhibitory effects of the crude extracts. Potential cell cytotoxicity of six different H. lupulus extracts were assayed on various cancer cells using MTT assay at 24, 48 and 72 h intervals. Methanol-1 extract has inhibited the cell proliferation with doses of 0.6-1 mg/mL in a time dependent (48 and 72 hours) manner in Hep3B cells with 70% inhibition, while inhibitory effect was not seen in colon cancer cells. Acetone extract has increased the cell proliferation at low doses of 0.1 mg/mL for 72 h in Hep3B cells and 0.1-0.2 mg/mL for 48 and 72 h in HT29 cells. The inhibitory effects of the extracts were compared by relative maximum activity values (V(max)) using proteases such as α-chymotrypsin, trypsin and papain, tyrosinase and β-lactamase (penicillinase).
authors: a Department of Chemistry, Natural Products and Drug Research Laboratory , Canakkale Onsekiz Mart University , Çanakkale , Turkey .
authors: Ferah
authors: F
authors: Cömert Önder
authors: a Department of Chemistry, Natural Products and Drug Research Laboratory , Canakkale Onsekiz Mart University , Çanakkale , Turkey .
authors: Mehmet
authors: M
authors: Ay
authors: b Department of Molecular Biology and Genetics , Balikesir University, Cagis Campus , Balikesir , Turkey .
authors: Sümeyye
authors: S
authors: Aydoğan Türkoğlu
authors: c Department of Biology , Balikesir University, Cagis Campus , Balikesir , Turkey , and.
authors: Feray
authors: F
authors: Tura Köçkar
authors: d Department of Chemistry , Gebze Institute of Technology , Gebze/Kocaeli , Turkey.
authors: Ayhan
authors: A
authors: Çelik
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D027582:Humulus / Q000737:chemistry*; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D065093:beta-Lactamase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; D065093:beta-Lactamase Inhibitors; D014442:Monophenol Monooxygenase; D010447:Peptide Hydrolases; D001618:beta-Lactamases
keywords: Antiproliferation; HT-29; Hep3B; Humulus lupulus; enzyme inhibition; hop female cones; proteases; tyrosinase; β-lactamase (penicillinase)