title: Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity.
abstract: A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F(522nm)/F(630nm)) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (Φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H(+) over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.
authors: Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University , Taiyuan 030006, People's Republic of China.
authors: Weifen
authors: W
authors: Niu
authors: Li
authors: L
authors: Fan
authors: Ming
authors: M
authors: Nan
authors: Zengbo
authors: Z
authors: Li
authors: Dongtao
authors: D
authors: Lu
authors: Man Shing
authors: MS
authors: Wong
authors: Shaomin
authors: S
authors: Shuang
authors: Chuan
authors: C
authors: Dong
mesh_terms: D000143:Acids / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003952:Diagnostic Imaging; D004926:Escherichia coli / Q000166:cytology*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry; D007680:Kidney Neoplasms / Q000473:pathology*; D011804:Quinolines / Q000737:chemistry
chemical_list: C000601531:1,1-dimethyl-2-(2-(quinolin-4-yl)vinyl)-1H-benzo(e)indole; D000143:Acids; D005456:Fluorescent Dyes; D007211:Indoles; D011804:Quinolines