title: Structure activity relationship of bergenin, p-hydroxybenzoyl bergenin, 11-O-galloylbergenin as potent antioxidant and urease inhibitor isolated from Bergenia ligulata.
abstract: Ethanol extract of the aerial parts of Bergenia ligulata was subjected to solvent-solvent separation followed by various chromatographic techniques that lead to isolation of bergenine (1), p-hydroxybenzoyl bergenin (2), 11-O-galloylbergenin (3) and methyl gallate (4) as major constituents. Ethyl acetate fraction showed a dose-dependent urease inhibitory pattern with IC50 value of 54μg/mL. Structures of compounds 1 and 3 were established by XRD and 2, 4 by NMR. All these compounds were subjected to DPPH scavenging activity, reducing power assay and urease inhibitory activity. The EC50 7.45 ± 0.2 μg/mL and 5.39 ± 0.28 μg/mL values in terms of antioxidant and reducing power, respectively, were less for 3. Compounds 1-3 showed moderate to significant urease inhibitory potential with IC50 57.1 ± 0.7, IC50 48.4 ± 0.3 and 38.6 ± 1.5. Antioxidant activities and urease inhibitory potential were investigated and compound 3 was found to be the most active.
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors: Anwar
authors: A
authors: Sadat
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors: Ghias
authors: G
authors: Uddin
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors: M
authors: M
authors: Alam
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors: Ashfaq
authors: A
authors: Ahmad
authors: b HEJ Research Institute of Chemistry, Karachi University , Karachi , Pakistan.
authors: Bina Shaheen
authors: BS
authors: Siddiqui
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D031995:Saxifragaceae / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: C556377:11-O-galloylbergenin; D000975:Antioxidants; D001578:Benzopyrans; D010936:Plant Extracts; C052082:methyl gallate; D005707:Gallic Acid; D014510:Urease; C006741:bergenin
keywords: Jack bean urease; antioxidant assay; peptic ulcers; reactive oxygen species