title: Polymorphisms of FtsK/SpoIIIE protein in Mycobacterium tuberculosis complex strains may affect both protein function and host immune reaction.
abstract: FtsK/SpoIIIE family ATPases are conserved throughout bacteria and are involved in the translocation of DNA and proteins through membrane-spanning pores. Through comparison of 26 whole-genome-sequenced M. tuberculosis complex (MTBC) strains downloaded from NCBI website, we found that FtsK/SpoIIIE protein presented polymorphisms. One M. bovis strain and three BCG strains even showed the two T cell epitopes missing. Then we chose 159 clinical M. tuberculosis isolates from China, amplified gene encoding FtsK/SpoIIIE protein (Rv3871) and compared the sequences. The results showed that there are polymorphisms existed in FtsK/SpoIIIE protein among MTBC, which may affect both protein function and host immune reaction. In addition, position 1497 could be used as a good phylogenetic marker for Beijing strains.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, P. R. China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003, China.
authors: Yi
authors: Y
authors: Jiang
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, P. R. China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003, China.
authors: Haican
authors: H
authors: Liu
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, P. R. China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003, China.
authors: Yan
authors: Y
authors: Qiu
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, P. R. China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003, China.
authors: Guilian
authors: G
authors: Li
authors: Beijing Center for Diseases Prevention and Control Beijing 100013, China.
authors: Xiangfeng
authors: X
authors: Dou
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, P. R. China ; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003, China.
authors: Kanglin
authors: K
authors: Wan
keywords: FtsK/SpoIIIE protein; Mycobacterium tuberculosis; polymorphisms