title: Use of RUNX2 expression to identify osteogenic progenitor cells derived from human embryonic stem cells.
abstract: We generated a RUNX2-yellow fluorescent protein (YFP) reporter system to study osteogenic development from human embryonic stem cells (hESCs). Our studies demonstrate the fidelity of YFP expression with expression of RUNX2 and other osteogenic genes in hESC-derived osteoprogenitor cells, as well as the osteogenic specificity of YFP signal. In vitro studies confirm that the hESC-derived YFP(+) cells have similar osteogenic phenotypes to osteoprogenitor cells generated from bone-marrow mesenchymal stem cells. In vivo studies demonstrate the hESC-derived YFP(+) cells can repair a calvarial defect in immunodeficient mice. Using the engineered hESCs, we monitored the osteogenic development and explored the roles of osteogenic supplements BMP2 and FGF9 in osteogenic differentiation of these hESCs in vitro. Taken together, this reporter system provides a novel system to monitor the osteogenic differentiation of hESCs and becomes useful to identify soluble agents and cell signaling pathways that mediate early stages of human bone development.
authors: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Li
authors: L
authors: Zou
authors: Diagnostic/Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Fahad K
authors: FK
authors: Kidwai
authors: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Ross A
authors: RA
authors: Kopher
authors: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Jason
authors: J
authors: Motl
authors: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
authors: Cory A
authors: CA
authors: Kellum
authors: Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.
authors: Jennifer J
authors: JJ
authors: Westendorf
authors: Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: kaufm020@umn.edu.
authors: Dan S
authors: DS
authors: Kaufman
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics* / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000235:genetics*; D010641:Phenotype
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit