title: Analysis of bone marrow aspiration fluid using automated blood cell counters.
abstract: Cytomorphological examination of aspirate smears remains the basic method to diagnose hematologic disorders and to evaluate treatment-related changes. Last-generation hematological analyzers can count, besides cells normally circulating in peripheral blood, some types of immature and abnormal cells, such as erythroblasts and immature granulocytes. The complex nature of bone marrow fluid, however, has prevented until now the routine utilization of blood cell counters in this area. Recent studies have shown the possibility of using bone marrow fluid as a substitute for peripheral blood for clinical tests in particular situations and for repetitive cytologic examinations in specific clinical and research fields.
authors: Research Center for the Development and Clinical Evaluation of Automated Methods in Hematology, Catholic University of Sacred Heart, Largo Agostino Gemelli 8, Rome 00193, Italy. Electronic address: giusdono@gmail.com.
authors: Giuseppe
authors: G
authors: d'Onofrio
authors: Research Center for the Development and Clinical Evaluation of Automated Methods in Hematology, Catholic University of Sacred Heart, Largo Agostino Gemelli 8, Rome 00193, Italy.
authors: Gina
authors: G
authors: Zini
mesh_terms: D000818:Animals; D001331:Automation; D001707:Biopsy, Needle; D001772:Blood Cell Count / Q000379:methods* / Q000639:trends; D001854:Bone Marrow Cells; D006801:Humans; D015203:Reproducibility of Results
keywords: Automated blood cell counters; Bone marrow analysis; Bone marrow aspirate; Erythroblast count; Flow cytometry; Reticulocytes; Toxicologic studies