title: High-density genetic linkage map construction and QTL mapping of grain shape and size in the wheat population Yanda1817 × Beinong6.
abstract: High-density genetic linkage maps are necessary for precisely mapping quantitative trait loci (QTLs) controlling grain shape and size in wheat. By applying the Infinium iSelect 9K SNP assay, we have constructed a high-density genetic linkage map with 269 F 8 recombinant inbred lines (RILs) developed between a Chinese cornerstone wheat breeding parental line Yanda1817 and a high-yielding line Beinong6. The map contains 2431 SNPs and 128 SSR & EST-SSR markers in a total coverage of 3213.2 cM with an average interval of 1.26 cM per marker. Eighty-eight QTLs for thousand-grain weight (TGW), grain length (GL), grain width (GW) and grain thickness (GT) were detected in nine ecological environments (Beijing, Shijiazhuang and Kaifeng) during five years between 2010-2014 by inclusive composite interval mapping (ICIM) (LOD ≥ 2.5). Among which, 17 QTLs for TGW were mapped on chromosomes 1A, 1B, 2A, 2B, 3A, 3B, 3D, 4A, 4D, 5A, 5B and 6B with phenotypic variations ranging from 2.62% to 12.08%. Four stable QTLs for TGW could be detected in five and seven environments, respectively. Thirty-two QTLs for GL were mapped on chromosomes 1B, 1D, 2A, 2B, 2D, 3B, 3D, 4A, 4B, 4D, 5A, 5B, 6B, 7A and 7B, with phenotypic variations ranging from 2.62% to 44.39%. QGl.cau-2A.2 can be detected in all the environments with the largest phenotypic variations, indicating that it is a major and stable QTL. For GW, 12 QTLs were identified with phenotypic variations range from 3.69% to 12.30%. We found 27 QTLs for GT with phenotypic variations ranged from 2.55% to 36.42%. In particular, QTL QGt.cau-5A.1 with phenotypic variations of 6.82-23.59% was detected in all the nine environments. Moreover, pleiotropic effects were detected for several QTL loci responsible for grain shape and size that could serve as target regions for fine mapping and marker assisted selection in wheat breeding programs.
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Qiu-Hong
authors: QH
authors: Wu
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yong-Xing
authors: YX
authors: Chen
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Sheng-Hui
authors: SH
authors: Zhou
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Lin
authors: L
authors: Fu
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Jiao-Jiao
authors: JJ
authors: Chen
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yao
authors: Y
authors: Xiao
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Dong
authors: D
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Shu-Hong
authors: SH
authors: Ouyang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Xiao-Jie
authors: XJ
authors: Zhao
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yu
authors: Y
authors: Cui
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: De-Yun
authors: DY
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yong
authors: Y
authors: Liang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Zhen-Zhong
authors: ZZ
authors: Wang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Jing-Zhong
authors: JZ
authors: Xie
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Jin-Xia
authors: JX
authors: Qin
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Guo-Xin
authors: GX
authors: Wang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: De-Lin
authors: DL
authors: Li
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yin-Lian
authors: YL
authors: Huang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Mei-Hua
authors: MH
authors: Yu
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Ping
authors: P
authors: Lu
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Li-Li
authors: LL
authors: Wang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Ling
authors: L
authors: Wang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Hao
authors: H
authors: Wang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Chen
authors: C
authors: Dang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Jie
authors: J
authors: Li
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Yan
authors: Y
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Hui-Ru
authors: HR
authors: Peng
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Cheng-Guo
authors: CG
authors: Yuan
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Ming-Shan
authors: MS
authors: You
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Qi-Xin
authors: QX
authors: Sun
authors: Department of Plant Sciences, University of California at Davis, Davis 95616, United States of America; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
authors: Ji-Rui
authors: JR
authors: Wang
authors: Beijing Academy of Agriculture and Forestry Sciences, Beijing 100197, China.
authors: Li-Xin
authors: LX
authors: Wang
authors: Department of Plant Sciences, University of California at Davis, Davis 95616, United States of America.
authors: Ming-Cheng
authors: MC
authors: Luo
authors: Beijing University of Agriculture, Beijing 102206, China.
authors: Jun
authors: J
authors: Han
authors: State Key Laboratory for Agrobiotechnology / Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China.
authors: Zhi-Yong
authors: ZY
authors: Liu
mesh_terms: D002874:Chromosome Mapping; D004777:Environment; D059647:Gene-Environment Interaction; D056726:Genetic Association Studies; D008040:Genetic Linkage; D018745:Genome, Plant; D023281:Genomics; D006801:Humans; D007178:Inbreeding; D018895:Microsatellite Repeats; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D014908:Triticum / Q000235:genetics*