title: Response of endothelial cells to decellularized extracellular matrix deposited by bone marrow mesenchymal stem cells.
abstract: OBJECTIVE
  Evaluate the behavior and function of human umbilical vein endothelial cells (HUVECs) on decellularized extracellular matrix (ECM) deposited by bone marrow mesenchymal stem cells (BMSCs).
  
  
  METHODS
  Prepared through chemical approach, decellularized ECM was characterized by use of immunofluorescence staining. The morphology, attachment, proliferation and migration of HUVECs cultured on six-well tissue culture plastic (TCP) and decellularized ECM were investigated.
  
  
  RESULTS
  Decellularized ECM was successfully prepared without three-dimensional architecture disruption. This biological scaffold is similar to nature vascular ECM, preserved various matrix proteins such as type I collagen, type III collagen and fibronection. HUVECs on decellularized ECM showed well attachment and regular arrangement. Decellularized ECM could also significantly enhance the migration and proliferation potential of HUVECs in contrast to TCP.
  
  
  CONCLUSIONS
  Deposited by BMSCs, ECM can affect the behavior of endothelial cell and could be used as a promising material in tissue engineering.
authors: Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology Guangzhou, Guangdong, China.
authors: Yue
authors: Y
authors: Xu
authors: Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology Guangzhou, Guangdong, China.
authors: Mengdie
authors: M
authors: Yan
authors: School of Engineering, Sun Yat-sen University Guangzhou, Guangdong, China.
authors: Yihong
authors: Y
authors: Gong
authors: Department of Burns Surgery, First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong, China.
authors: Lei
authors: L
authors: Chen
authors: Department of Biomedical Engineering, Michigan Technological University 1400 Townsend Drive, Houghton, Michigan, USA.
authors: Feng
authors: F
authors: Zhao
authors: Department of Stomatology, Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong, China.
authors: Zhaoqiang
authors: Z
authors: Zhang
keywords: Decellularized extracellular matrix; tissue engineering; vascularization