title: Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24.
abstract: Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Chune
authors: C
authors: Li
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Jinshui
authors: J
authors: Yang
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Xin
authors: X
authors: Wang
authors: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico, DF, Mexico.
authors: Entao
authors: E
authors: Wang
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Baozhen
authors: B
authors: Li
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Ruoxue
authors: R
authors: He
authors: State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China. Electronic address: hlyuan@cau.edu.cn.
authors: Hongli
authors: H
authors: Yuan
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism; D058440:Denitrification; D052836:Heterotrophic Processes; D058465:Nitrification; D009584:Nitrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater
chemical_list: D000641:Ammonia; D009584:Nitrogen; D010710:Phosphates; D062065:Wastewater
keywords: Aerobic denitrification; Heterotrophic nitrification; Nitrogen removal; Phosphate accumulating bacterium; Pseudomonas stutzeri YG-24