title: Modulation of domain-domain interaction and protein function by a charged linker: a case study of mycobacteriophage D29 endolysin.
abstract: Phage-encoded cell wall peptidoglycan hydrolyzing enzymes, called endolysins, are essential for efficient release of virions from bacteria, and show species-specific killing of the host. We have demonstrated previously that the interaction between N-terminal catalytic and C-terminal cell wall binding domains of mycobacteriophage D29 endolysin makes the enzyme inactive in Escherichiacoli. Here, we demonstrate that such interaction occurs intramolecularly and is facilitated by a charged linker that connects the two domains. We also show that linker composition is crucial for the inactivation of PG hydrolase in E. coli. Such knowledge will immensely help in bioengineering of endolysins with narrow or broad spectrum antimicrobial activity.
authors: Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India.
authors: Amol Arunrao
authors: AA
authors: Pohane
authors: Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India.
authors: Neelam Devidas
authors: ND
authors: Patidar
authors: Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India. Electronic address: vikas@iiserb.ac.in.
authors: Vikas
authors: V
authors: Jain
mesh_terms: D000595:Amino Acid Sequence; D016384:Consensus Sequence; D010450:Endopeptidases / Q000096:biosynthesis / Q000737:chemistry*; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009160:Mycobacteriophages; D010457:Peptidoglycan / Q000737:chemistry; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry*
chemical_list: D010457:Peptidoglycan; D014764:Viral Proteins; D010450:Endopeptidases; C001143:endolysin
keywords: Activity regulation; Bacteriophage; Cell wall peptidoglycan; Charged linker; Domain–domain interaction; Endolysin