title: Multidimensional visualization of healthy and sensitized rabbit knee tissues by means of confocal microscopy.
abstract: This study combines several fluorescence detection methods to distinguish structural features of the synovium and cartilage tissues and to visualize the localization of endogenous porphyrins in the sensitized tissues. Specimens of synovium and cartilage tissues obtained from rabbits with antigen-induced monoarthritis after intra-articular 5-aminolevulinic acid methyl ester injection and those from healthy rabbits were investigated ex vivo by means of fluorescence spectroscopy, fluorescence intensity, and lifetime microscopy. The presence of endogenous porphyrins was confirmed with the fluorescence spectra measured on sliced sensitized specimens. Application of the lifetime-gating method on fast fluorescence lifetime imaging microscopy images, allowed separate visualization of tissue structures possessing different average lifetimes. The presence of the structures has been validated by histopathological imaging based on conventional rapid hematoxylin–eosin staining of the specimens. The fluorescence lifetime of endogenous protoporphyrin IX has been assessed and employed for visualization of sensitized tissues.
authors: The National Cancer Institute, P. Baublio 3b, Vilnius LT 08406, LithuaniabState Research Institute, Center for Innovative Medicine, Žygimantu 9, Vilnius LT 01102, Lithuania.
authors: Romualdas
authors: R
authors: Rudys
authors: Vilnius University, Biophotonics Group of Laser Research Center, Faculty of Physics, Sauletekio 9, Building 3, Vilnius LT-10222, Lithuania.
authors: Saulius
authors: S
authors: Bagdonas
authors: State Research Institute, Center for Innovative Medicine, Žygimantu 9, Vilnius LT 01102, Lithuania.
authors: Gailutė
authors: G
authors: Kirdaitė
authors: State Research Institute, Center for Innovative Medicine, Žygimantu 9, Vilnius LT 01102, Lithuania.
authors: Jurga
authors: J
authors: Papečkienė
authors: The National Cancer Institute, P. Baublio 3b, Vilnius LT 08406, LithuaniacVilnius University, Biophotonics Group of Laser Research Center, Faculty of Physics, Sauletekio 9, Building 3, Vilnius LT-10222, Lithuania.
authors: Ričardas
authors: R
authors: Rotomskis
mesh_terms: D000622:Aminolevulinic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D002356:Cartilage / Q000473:pathology; D002682:Chinchilla; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D006416:Hematoxylin / Q000737:chemistry; D006614:Hindlimb / Q000473:pathology*; D007596:Joints / Q000473:pathology*; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D017319:Photosensitizing Agents / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D011524:Protoporphyrins / Q000737:chemistry; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013583:Synovial Membrane / Q000473:pathology
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D011524:Protoporphyrins; C114767:delta-aminolevulinic acid methyl ester; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin