title: Human dental pulp mesenchymal stem cells isolation and osteoblast differentiation.
abstract: OBJECTIVE
  This study was focused on the isolation and characterization of mesenchymal stem cells (MSCs) from human dental pulp (DPSC).
  
  
  METHODS
  The study was performed in the Department for Oral and Cranio-Maxillo- Facial Surgey Hamad Medical Corporation, Doha, Qatar and Weill Cornell Medical Colleague Doha, Qatar, in period 2010-2011. Dental pulp was extracted from premolars and third molars of 19 healthy patients. The pulp was digested in a solution of 3 mg/mL collagenase type I and 4 mg/mL dispase for 1 hour at 37C. After filtration, cells were cultured in Dulbecco's Modified Eagle Medium (DMEM Low Glucoses) with 20% Fetal Bovine Serum (FBS), 2mM L-glutamine and antibiotics (100 U/mL penicillin, 100 ug/mL streptomycin) at 37 °C under 5% CO2. Cultures were treated with osteoinductive medium for differentiation MSC in to the osteoblast cell line. Staining with Alizarin red were used for the detection of the osteoblast production and calcification new formed tissue.
  
  
  RESULTS
  On the total of three out of 19 patients it was possible to isolate DPMSCs after 2 to 3 weeks: in one patient it was not possible to expand MSCs because of infection, and in other two patients positive Alizarin red staining reaction showed osteogenic differentiation capability and strong mineralization in vitro.
  
  
  CONCLUSIONS
  The main advantage of using DPSC is absence of morbidity. MSCs could be isolated noninvasively from teeth, routinely extracted in the clinic and discarded as medical waste. Standardization of clinical and laboratory protocols for DPMSCs isolation and team work coordination could lead to significantly improved result.
authors: Department for Oral and Cranio-Maxillo-Facial Surgery, Hamad Medical Corporation, Doha, Qatar, Weill Cornell Medical College, Doha, Qatar.
authors: Moustafa
authors: M
authors: Alkhalil
authors: Department for Oral and Cranio-Maxillo-Facial Surgery, Hamad Medical Corporation, Doha, Qatar, Weill Cornell Medical College, Doha, Qatar.
authors: Amer
authors: A
authors: Smajilagić
authors: Institute for Biology and Human Genetic, School of Medicine University of Sarajevo , Sarajevo, Bosnia and Herzegovina.
authors: Amira
authors: A
authors: Redžić
mesh_terms: D000293:Adolescent; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008964:Molar, Third / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology*; D055815:Young Adult
chemical_list: D003470:Culture Media