title: Rapid isolation and purification of phorbol esters from Jatropha curcas by high-speed countercurrent chromatography.
abstract: In this work, a high-speed countercurrent chromatography (HSCCC) method was established for the preparation of phorbol esters (PEs) from Jatropha curcas. n-Hexane-ethyl acetate-methanol-water (1.5:1.5:1.2:0.5, v/v) was selected as the optimum two-phase solvent system to separate and purify jatropha factor C1 (JC1) with a purity of 85.2%, as determined by HPLC, and to obtain a mixture containing four or five PEs. Subsequently, continuous semipreparative HPLC was applied to further purify JC1 (99.8% as determined by HPLC). In addition, UPLC-PDA and UPLC-MS were established and successfully used to evaluate the isolated JC1 and PE-rich crude extract. The purity of JC1 was only 87.8% by UPLC-UV. A peak (a compound highly similar to JC1) was indentified as the isomer of JC1 by comparing the characteristic UV absorption and MS spectra. Meanwhile, this strategy was also applied to analyze the PE-rich crude extract from J. curcas. It is interesting that there may be more than 15 PEs according to the same quasi-molecular ion peaks, highly similar sequence-specific fragment ions, and similar UV absorption spectrum.
authors: †National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610064, People's Republic of China.
authors: Wan
authors: W
authors: Hua
authors: ‡Pharmacy College, Chengdu University of Traditional Chinese Medicine, No. 1166, Liutai Road, Chengdu 611137, People's Republic of China.
authors: Huiling
authors: H
authors: Hu
authors: †National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610064, People's Republic of China.
authors: Fang
authors: F
authors: Chen
authors: †National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610064, People's Republic of China.
authors: Lin
authors: L
authors: Tang
authors: †National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610064, People's Republic of China.
authors: Tong
authors: T
authors: Peng
authors: †National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610064, People's Republic of China.
authors: Zhanguo
authors: Z
authors: Wang
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D031286:Jatropha / Q000737:chemistry*; D010703:Phorbol Esters / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D010703:Phorbol Esters; D010936:Plant Extracts
keywords: 12-deoxy-16-hydroxyphorbol diesters; HSCCC; Jatropha curcas L.; UPLC−MS; jatropha factor C1