title: Streptomyces maoxianensis sp. nov., a novel actinomycete isolated from soil in Maoxian, China.
abstract: A novel actinomycete, designated strain NEAU-Spg16(T), was isolated from a soil sample from a pine forest in Songpinggou, Maoxian, southwest China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-Spg16(T). The cell wall peptidoglycan was found to contain LL-diaminopimelic acid and glycine. The major menaquinones were identified as MK-9(H8), MK-9(H4) and MK-9(H6). The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid. The major fatty acids were identified as iso-C(16:0), C(18:0), C(16:0) and iso-C(15:0). 16S rRNA gene sequence similarity studies showed that strain NEAU-Spg16(T) belongs to the genus Streptomyces with the highest sequence similarity to Streptomyces nitrosporeus DSM 40023(T) (98.6%). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that it is most closely related to Streptomyces scopuliridis DSM 41917(T) (98.2% sequence similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-Spg16(T) can be clearly differentiated from S. scopuliridis DSM 41917(T) and S. nitrosporeus DSM 40023(T). Therefore, it is concluded that strain NEAU-Spg16(T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces maoxianensis sp. nov. is proposed. The type stain is NEAU-Spg16(T) (=CGMCC 4.7139(T) = DSM 42137(T)).
authors: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
authors: Xuejiao
authors: X
authors: Guan
authors: Chongxi
authors: C
authors: Liu
authors: Junwei
authors: J
authors: Zhao
authors: Baozhu
authors: B
authors: Fang
authors: Yuejing
authors: Y
authors: Zhang
authors: Lianjie
authors: L
authors: Li
authors: Pinjiao
authors: P
authors: Jin
authors: Xiangjing
authors: X
authors: Wang
authors: Wensheng
authors: W
authors: Xiang
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S