title: Improvement of ethanol production by ethanol-tolerant Saccharomyces cerevisiae UVNR56.
abstract: Ethanol tolerance is one of the important characteristics of ethanol-producing yeast. This study focused on the improvement of ethanol tolerance of Saccharomyces cerevisiae NR1 for enhancing ethanol production by random UV-C mutagenesis. One ethanol-tolerant mutant, UVNR56, displayed a significantly improved ethanol tolerance in the presence of 15% (v/v) ethanol and showed a considerably higher viability during ethanol fermentation from sugarcane molasses and sugarcane molasses with initial ethanol supplementation. A maximum ethanol concentration produced from molasses medium at 37°C by UVNR56 was 10.3% (v/v), productivity of 1.7 g/l/h and a theoretical yield of 98.7%, while the corresponding values for the wild-type were 8.6% (v/v), 1.4 g/l/h and 83.3%, respectively. In addition, during molasses fermentation under initial supplementation of 5% (v/v) ethanol, the maximum ethanol concentration and productivity of UVNR56 was 25.7% and 42.9% higher than the wild-type, respectively.
authors: Microbial Biotechnology Unit, Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom, 73140 Thailand.
authors: Sutticha Na-Ranong
authors: SN
authors: Thammasittirong
authors: Department of Science, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom, 73140 Thailand.
authors: Thanawan
authors: T
authors: Thirasaktana
authors: Microbial Biotechnology Unit, Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom, 73140 Thailand.
authors: Anon
authors: A
authors: Thammasittirong
authors: Microbial Biotechnology Unit, Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom, 73140 Thailand.
authors: Malee
authors: M
authors: Srisodsuk
keywords: Ethanol production; Ethanol tolerance; Molasses; Mutagenesis; Saccharomyces cerevisiae