title: Measurement of plasma gentamicin concentrations postchemical ciliary body ablation in dogs with chronic glaucoma.
abstract: OBJECTIVE
  To investigate the absorption of gentamicin into the plasma after an intravitreal injection in dogs and to report the success rate of this procedure in lowering the intraocular pressure.
  
  
  METHODS
  Twenty-four client-owned dogs with chronic, end-stage glaucoma.
  
  
  METHODS
  Dogs received a unilateral (22) or bilateral (2) intravitreal injection of 25-40 mg of gentamicin (mean ± SD dose 2.57 ± 1.65 mg/kg and range 0.61-7.50 mg/kg) and 1 mg of dexamethasone per eye. Blood samples were collected at various time points following the intravitreal injection. Plasma concentrations of gentamicin were determined by liquid chromatography and mass spectrometry.
  
  
  RESULTS
  The total plasma concentration of gentamicin ranged from 0.21 to 9.71 μg/mL (mean ± SD 2.15 ± 2.03). The mean gentamicin CMAX was 2.29 μg/mL at 2.54 h with a terminal half-life of 9.8 h. The success rate of the chemical ablation procedure was 86.4% (19/22 eyes) in dogs that had at least 1 month of follow-up.
  
  
  CONCLUSIONS
  Intravitreal injection of gentamicin in eyes with chronic glaucoma resulted in detectable plasma levels in dogs and was successful in lowering the intraocular pressure in 86.4% of the eyes after the first procedure.
authors: Department of Clinical Sciences, Kansas State University, Manhattan, KS, USA.
authors: Amy J
authors: AJ
authors: Rankin
authors: Department of Clinical Sciences, Kansas State University, Manhattan, KS, USA.
authors: Rick
authors: R
authors: Lanuza
authors: Department of Anatomy and Physiology, Kansas State University, Manhattan, KS, USA.
authors: Butch
authors: B
authors: KuKanich
authors: Department of Clinical Studies, University of Pennsylvania, Philadelphia, PA, USA.
authors: William C
authors: WC
authors: Crumley
authors: Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, USA.
authors: Jonathan D
authors: JD
authors: Pucket
authors: Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA, USA.
authors: Rachel A
authors: RA
authors: Allbaugh
authors: Department of Clinical Sciences, Kansas State University, Manhattan, KS, USA.
authors: Jessica M
authors: JM
authors: Meekins
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use; D019540:Area Under Curve; D002924:Ciliary Body / Q000473:pathology*; D004283:Dog Diseases / Q000097:blood / Q000628:therapy*; D004285:Dogs; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005901:Glaucoma / Q000628:therapy / Q000662:veterinary*; D006207:Half-Life; D058449:Intravitreal Injections / Q000662:veterinary; D008297:Male
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins
keywords: canine; chemical ablation; ciliary body ablation; gentamicin; glaucoma; plasma