title: Increase of DC-LAMP+ mature dendritic cell subsets in dermatopathic lymphadenitis of mycosis fungoides.
abstract: BACKGROUND
  Little is known about the immunological milieu of the skin-draining lymph nodes (LNs) in mycosis fungoides (MF).
  
  
  OBJECTIVE
  We studied dendritic cell (DC) subsets in the dermatopathic lymphadenitis of MF patients.
  
  
  METHODS
  We immunohistochemically examined DC subsets and their distribution in 16 LN samples from 14 patients with MF (N1 LN, eight patients; N2, four; and N3, four), and we compared them with non-metastatic sentinel LNs from eight patients with melanoma.
  
  
  RESULTS
  The number of S-100 protein+ DCs was markedly increased in the LNs from the MF patients and the major component was DC-LAMP+ mature DCs in the outer and paracortex areas, where DC-SIGN+ immature DCs were relatively decreased in proportion. In contrast, DC-SIGN+ cells were relatively increased in proportion compared to DC-LAMP+ cells in the medulla. Although no significant difference was observed in the proportions of CD1a+ or Langerin+ DCs among the N1, N2, and N3 nodes, CD163+ M2-type macrophages were increased in number in the N2 and N3 nodes.
  
  
  CONCLUSIONS
  Our observations indicate that mature DCs accumulate in the outer and paracortex areas in dermatopathic lymphadenitis and M2-type macrophages might increase in number during disease progression.
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan, Department of Dermatology, Kurashiki Daiichi Hospital, Kurashiki 710-0826, Japan.
authors: Kotaro
authors: K
authors: Tada
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Toshihisa
authors: T
authors: Hamada
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan, Department of Dermatology, Okayama Medical Center, Okayama 701-1192, Japan.
authors: Kenji
authors: K
authors: Asagoe
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Hiroshi
authors: H
authors: Umemura
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Kazuko
authors: K
authors: Mizuno-Ikeda
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Yumi
authors: Y
authors: Aoyama
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Masaki
authors: M
authors: Otsuka
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Osamu
authors: O
authors: Yamasaki
authors: Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors: Keiji
authors: K
authors: Iwatsuki
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D002452:Cell Count; D003713:Dendritic Cells / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000032:analysis; D008198:Lymph Nodes / Q000473:pathology*; D008199:Lymphadenitis / Q000209:etiology / Q000473:pathology*; D059666:Lysosomal-Associated Membrane Protein 3 / Q000032:analysis*; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008545:Melanoma / Q000473:pathology*; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000150:complications / Q000473:pathology*; D009367:Neoplasm Staging; D011956:Receptors, Cell Surface / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000150:complications / Q000473:pathology*; D000098902:CD163 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D059666:Lysosomal-Associated Membrane Protein 3; D011956:Receptors, Cell Surface; D009418:S100 Proteins; D000098902:CD163 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
keywords: DC-LAMP; DC-SIGN; M2-type macrophage; Mycosis fungoides; dendritic cell; dermatopathic lymphadenitis