title: The SENP7 SUMO-Protease Presents a Module of Two HP1 Interaction Motifs that Locks HP1 Protein at Pericentric Heterochromatin.
abstract: HP1 enrichment at pericentric heterochromatin is essential for proper chromosome segregation. While H3K9me3 is thought to be a major contributor to HP1 enrichment at pericentric domains, in mouse cells, the SUMO-protease SENP7 is required in addition to H3K9me3. How this is achieved remains elusive. Here, we find that loss of SENP7 leads to an increased time spent in mitosis. Furthermore, we reveal that a short module comprising two consecutive HP1 interaction motifs on SENP7 is the determinant for HP1 enrichment and acts by restricting HP1 mobility at pericentric domains. We propose a mechanism for maintenance of HP1 enrichment in which this module functions on top of H3K9me3 to lock contiguous HP1 molecules already docked on H3K9me3-modified nucleosomes. H3K9me3 would thus promote HP1 enrichment only if a locking system is in place. This mechanism may apply to other nuclear domains to contribute to the control of genome plasticity and integrity.
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France.
authors: Kelly
authors: K
authors: Romeo
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France.
authors: Yann
authors: Y
authors: Louault
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France.
authors: Sylvain
authors: S
authors: Cantaloube
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France.
authors: Isabelle
authors: I
authors: Loiodice
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France. Electronic address: genevieve.almouzni@curie.fr.
authors: Geneviève
authors: G
authors: Almouzni
authors: Institut Curie, Centre de Recherche, Paris 75248, France; Centre National de la Recherche Scientifique (CNRS), UMR3664, Paris 75248, France; Equipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie (UPMC), UMR3664, Paris 75248, France; Sorbonne University, PSL, Paris 75005, France. Electronic address: jpquivy@curie.fr.
authors: Jean-Pierre
authors: JP
authors: Quivy