title: Structural characterization and antioxidant activity of a heteropolysaccharide from Ganoderma capense.
abstract: In this work, crude polysaccharide extracts were obtained from mycelia of the edible fungus Ganoderma capense (Lloyd) Teng. After removal of proteins by the Sevage method, fractionation and purification by anion-exchange and gel-permeation chromatography, a polysaccharide (GCPB-3) was isolated. The relative molecular weight of GCPB-3 was 124kDa determined by high performance gel permeation chromatography (HPGPC). The homogeneous polysaccharide was composed of d-xylose and l-arabinose in the ratio of 1:1, and showed a specific optical rotation of [α]D(25)=+145°(c 1.0, H2O). Its structural features were determined by monosaccharide analysis, partial acid hydrolysis, methylation analysis, periodic acid oxidation, gas chromatography-mass spectrometry (GC-MS), Fourier transform-infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy ((1)H, (13)C, HMQC and HMBC). The results characterized GCPB-3 as a heteropolysaccharide with backbone consisting of β-l-Arap and β-d-Xylp, linked with 1→4 sugar bonds. Interestingly, GCPB-3 showed some DPPH•- and hydroxy-radical scavenging activities.
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors: Pan
authors: P
authors: Yi
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors: Naisheng
authors: N
authors: Li
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors: Jian-Bo
authors: JB
authors: Wan
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors: Dezhi
authors: D
authors: Zhang
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors: Mingya
authors: M
authors: Li
authors: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address: ycybridge@163.com.
authors: Chunyan
authors: C
authors: Yan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D010504:Periodic Acid / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D010504:Periodic Acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity.; Ganoderma capense; Polysaccharide; Structural characterization