title: Human telomerase gene and high-risk human papillomavirus infection are related to cervical intraepithelial neoplasia.
abstract: Our aims were to evaluate the clinical performance of human telomerase RNA gene component (hTERC gene) amplification assay with high-risk human papillomavirus (HR-HPV) DNA test of Hybrid Capture 2 DNA test (HC2), for the detection of high grade cervical precancerous lesions and cancer (CIN 2+). In addition, the association shown between hTERC gene amplification and HPV DNA test positive in women with and without cervical neoplasia was assessed. There were 92 women who underwent cytology, HR-HPV DNA test, hTERC gene amplification test, colposcopy and biopsy. We compared the clinical performance of hTERC gene test along with HR-HPV DNA test of women with colposcopy and routine screening. The samples were histology- confirmed high-grade cervical intraepithelial neoplasia (CIN 2) or worse (CIN2+) as the positive criterion. The test of hTERC gene showed the hTERC gene amplification positivity increased with the severity of histological abnormality and cytological abnormality. The test of hTERC gene showed higher specificity than HR-HPV DNA test for high-grade lesions (84.4% versus 50%) and also higher positive predictive value (90.4% versus 76.5%). Our results predicted that hTERC gene amplification demonstrated more specific performance for predicting the risk of progression and offer a strong potential as a tool for triage in cervical cancer screening, with the limited sensitive as HR-HPV DNA test.
authors: Department of Gynecology and Obstetrics, Chinese PLA General Hospital and Chinese PLA Medical School, Shanxi, China E-mail : liyalisci@126.com.
authors: Xu-Ye
authors: XY
authors: Zhao
authors: Yong
authors: Y
authors: Cui
authors: Shu-Fang
authors: SF
authors: Jiang
authors: Ke-Jun
authors: KJ
authors: Liu
authors: Hai-Qiong
authors: HQ
authors: Han
authors: Xiao-Su
authors: XS
authors: Liu
authors: Yali
authors: Y
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D003127:Colposcopy; D003430:Cross-Sectional Studies; D003581:Cytodiagnosis; D004279:DNA, Viral / Q000235:genetics; D055088:Early Detection of Cancer; D005260:Female; D005500:Follow-Up Studies; D005784:Gene Amplification; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D011379:Prognosis; D012313:RNA / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D014626:Vaginal Smears; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000235:genetics / Q000821:virology
chemical_list: D004279:DNA, Viral; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase