title: Esterases as stereoselective biocatalysts.
abstract: Non-lypolitic esterases are carboxylester hydrolases with preference for the hydrolysis of water-soluble esters bearing short-chain acyl residues. The potential of esterases as enantioselective biocatalysts has enlarged in the last few years due to the progresses achieved in different areas, such as screening methodologies, overproduction of recombinant esterases, structural information useful for understanding the rational behind enantioselectivity, and efficient methods in protein engineering. Contributions of these complementary know-hows to the development of new robust enantioselective esterases are critically discussed in this review.
authors: Department of Food, Environmental and Nutritional Sciences (DEFENS), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors: Diego
authors: D
authors: Romano
authors: Department of Food, Environmental and Nutritional Sciences (DEFENS), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors: Francesco
authors: F
authors: Bonomi
authors: Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus do Pici, Postal Box 6044, 60455-970 Fortaleza, Ceará, Brazil.
authors: Marcos Carlos
authors: MC
authors: de Mattos
authors: Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus do Pici, Postal Box 6044, 60455-970 Fortaleza, Ceará, Brazil.
authors: Thiago
authors: T
authors: de Sousa Fonseca
authors: Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus do Pici, Postal Box 6044, 60455-970 Fortaleza, Ceará, Brazil.
authors: Maria da Conceição Ferreira
authors: Mda C
authors: de Oliveira
authors: Department of Food, Environmental and Nutritional Sciences (DEFENS), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
authors: Francesco
authors: F
authors: Molinari
mesh_terms: D001709:Biotechnology; D004950:Esterases; D015202:Protein Engineering; D013237:Stereoisomerism
chemical_list: D004950:Esterases
keywords: Biocatalysis; Carboxylester hydrolases; Enantioselectivity; Esterases; Kinetic resolution; Medium engineering; Protein engineering