title: Humoral immune responses to Candida albicans complement receptor 3-related protein in the atopic subjects with vulvovaginal candidiasis. Novel sensitive marker for Candida infection.
abstract: In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral response against Candida CR3-RP. The most significant have been IgM and IgA isotype antibodies (33 and 47% positive cases, respectively). The quantitative evaluation of anti-CR3RP isotype antibodies was confronted with results of commercial ELISA anti-C. albicans antibodies diagnostics based on C. albicans cell wall mannan and β-glucan antigens, the most significant correlation being observed with anti-CR3-RP IgM and anti-β-D-glucan IgM (r(2) = 0.624) followed by isotype IgA (r(2) = 0.381). The immunogenicity and immunoreactivity of CR3RP antigen in RVC patients' sera had been evaluated with regard to the results reached by counterimmunoelectrophoresis and heterogeneous enzyme immunoassay. Obviously, synthetically prepared CR3-RP mimicking the Candida cell-wall-derived structure moiety represents a promising immunological tool not only for Candida serodiagnostics, but also prospectively for follow-up of targeted antifungal therapy and as promising Candida vaccine candidate.
authors: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravská cesta 9 Bratislava, Slovakia ema.paulovicova@savba.sk.
authors: Ema
authors: E
authors: Paulovičová
authors: Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Slovakia, Slovakia.
authors: Helena
authors: H
authors: Bujdáková
authors: Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Slovakia, Slovakia.
authors: Jarmila
authors: J
authors: Chupáčová
authors: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravská cesta 9 Bratislava, Slovakia.
authors: Lucia
authors: L
authors: Paulovičová
authors: Faculty Hospital of Comenius University, Department of Clinical Immunology and Allergy, Bratislava, Slovakia.
authors: Pavol
authors: P
authors: Kertys
authors: Department of Clinical Immunology and Allergy, Oncology Institute of St. Elisabeth, Bratislava, Slovakia.
authors: Martin
authors: M
authors: Hrubiško
mesh_terms: D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D005656:Fungal Proteins / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M
keywords: CR3-related protein; Candida albicans; glucan; mannan; recurrent vulvovaginal candidiasis