title: Serum tryptophan and its metabolites in female dogs undergoing ovariohysterectomy as treatment of pyometra or as elective spay surgery.
abstract: This study compares serum concentrations of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), and indoleamine 2,3-dioxygenase (IDO) activity in healthy bitches and bitches with bacterial uterine infection (pyometra). The effects of surgery were also assessed by measuring these variables in both groups of dogs before and after ovariohysterectomy. Presurgery, mean (±standard deviation) TRP, KYN, and KYNA concentrations and IDO activity were 68.44 ± 21.77, 2.00 ± 0.33, 112.11 ± 111.91 μmol/L, and 29.22 ± 10.10, respectively, in the healthy dogs; and 40.16 ± 12.11, 8.27 ± 3.94, 411.11 ± 199.60 μmol/L, and 205.92 ± 154.20, respectively, in the dogs with pyometra. Tryptophan and KYN levels had normalized on suture removal (10 days after surgery) though IDO activity and KYNA concentrations remained elevated during the postoperative period compared with presurgery values in both study groups. Our results suggest that KYNA concentrations and IDO activity could be useful indicators of the inflammation induced by pyometra and could be also used to monitor recovery following ovariohysterectomy in both healthy dogs and dogs with pyometra.
authors: Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland. Electronic address: roman.dabrowski@up.lublin.pl.
authors: Roman
authors: R
authors: Dąbrowski
authors: Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors: Ragnvi
authors: R
authors: Hagman
authors: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universidad Autónoma de Barcelona, Barcelona, Spain.
authors: Asta
authors: A
authors: Tvarijonaviciute
authors: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universidad Autónoma de Barcelona, Barcelona, Spain.
authors: Josep
authors: J
authors: Pastor
authors: Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
authors: Tomasz
authors: T
authors: Kocki
authors: Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
authors: Waldemar A
authors: WA
authors: Turski
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs / Q000097:blood; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D007044:Hysterectomy / Q000662:veterinary*; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000097:blood; D007736:Kynurenic Acid / Q000097:blood; D007737:Kynurenine / Q000097:blood; D010052:Ovariectomy / Q000662:veterinary*; D055112:Pyometra / Q000097:blood / Q000601:surgery / Q000662:veterinary*; D012016:Reference Values; D014364:Tryptophan / Q000097:blood*; D014599:Uterus / Q000382:microbiology
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine; D014364:Tryptophan; D007736:Kynurenic Acid
keywords: Biomarkers; Bitches; IDO; OHE; Pyometra; Tryptophan