title: Fibro-check: a combination of direct and indirect markers for liver fibrosis staging in chronic hepatitis C patients.
abstract: BACKGROUND AND RATIONALE FOR THE STUDY: The assessment of liver fibrosis provides useful information not only for diagnosis but also for therapeutic decision. This study was concerned with determining the levels of collagen III and its degrading enzyme matrix metalloproteinase-1 (MMP-1) as direct and complementary markers for liver fibrosis staging.
  
  
  RESULTS
  A total of 269 chronic hepatitis C patients constituted this study. Western blotting was used for identifying collagen III and MMP-1 in serum samples. As a result, collagen III and MMP-1 were identified, respectively, at 70 and 245 kDa using their respective mono-specific antibodies. These two markers were quantified in sera of patients using ELISA. Next, Fibro-check was constructed combining collagen III and MMP-1 together with other indirect markers which reflect alteration in hepatic functions that proved useful to stage liver fibrosis. Fibro-check produced area under the receiver-operating characteristic curve (AUC) 0.91 and 0.83 for significant (F2-F4) and cirrhosis (F4), respectively. Additionally, we estimated the performance of Fibro-check in comparison with aspartate to platelet ratio index (APRI) and fibrosis index. Fibro-check seems to be more efficient than both of them. Fibro-check was then applied to the validation study to test its accuracy and reproducibility showing AUCs 0.90 for F2-F4 and 0.86 for F4.
  
  
  CONCLUSIONS
  Fibro-check combining 'direct' and 'indirect' markers using a mathematical formula may improve the staging of liver fibrosis with a high degree of accuracy and seems more efficient than APRI and Fibrosis index in this group of Egyptian patients.
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Abdelfattah M
authors: AM
authors: Attallah
authors: Faculty of Science, Mansoura University, Mansoura, Egypt.
authors: Mohamed
authors: M
authors: El-Far
authors: Faculty of Science, Damietta University, New Damietta, Egypt.
authors: Camelia A
authors: CA
authors: Abdel Malak
authors: Faculty of Science, Helwan University, Cairo, Egypt.
authors: Mohamed M
authors: MM
authors: Omran
authors: Tropical Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors: Khaled
authors: K
authors: Farid
authors: Faculty of Science, Port-Said University, Port-Said, Egypt.
authors: Mostafa A
authors: MA
authors: Hussien
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Mohamed S
authors: MS
authors: Albannan
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Ahmed A
authors: AA
authors: Attallah
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Mohamed S
authors: MS
authors: Elbendary
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Dalia A
authors: DA
authors: Elbesh
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Noha A
authors: NA
authors: Elmenier
authors: Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt.
authors: Mohamed O
authors: MO
authors: Abdallah
mesh_terms: D000328:Adult; D019540:Area Under Curve; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D004796:Clinical Enzyme Tests; D024061:Collagen Type III / Q000097:blood*; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D008111:Liver Function Tests / Q000379:methods*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000097:blood*; D008875:Middle Aged; D010976:Platelet Count; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D015203:Reproducibility of Results; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D024061:Collagen Type III; D001219:Aspartate Aminotransferases; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1