title: Multilocus sequence typing of a dairy-associated Leuconostoc mesenteroides population reveals clonal structure with intragenic homologous recombination.
abstract: Leuconostoc mesenteroides strains play an important role in food fermentation. In this study, 136 strains from different dairy products in China and Mongolia were examined by multilocus sequence typing of 9 housekeeping genes. In total, 82 polymorphic sites were detected among the 9 loci. The number of polymorphic nucleotide sites varied between 4 (dnaA) and 18 (uvrC), whereas the nucleotide diversity per site among the 9 genes varied from 0.00379 in dnaA to 0.01195 in uvrC, suggesting a relatively low level of sequence diversity. For the recombination measurement, incongruence in the trees based on a single gene and concatenated sequences of all sequencing types were observed, indicative of extensive intragenic homologous recombination. The overall relatedness built by minimum spanning trees showed no clear relationship between the clonal complexes and either isolation source or sampling location of the strains. Our study presents, for the first time, the population structure of Leuc. mesenteroides strains of dairy origin.
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Wenyi
authors: W
authors: Zhang
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Wenjun
authors: W
authors: Liu
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Yuqing
authors: Y
authors: Song
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Haiyan
authors: H
authors: Xu
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Bilige
authors: B
authors: Menghe
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
authors: Heping
authors: H
authors: Zhang
authors: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China. Electronic address: sunzhihong78@163.com.
authors: Zhihong
authors: Z
authors: Sun
mesh_terms: D000483:Alleles; D002681:China; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D005798:Genes, Bacterial; D056426:Genetic Loci; D014644:Genetic Variation; D059765:Homologous Recombination; D007934:Leuconostoc / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008986:Mongolia; D058885:Multilocus Sequence Typing / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial
keywords: Leuconostoc mesenteroides; clonal structure; homologous recombination; multilocus sequence typing