title: Synergistic toxicity of gentamicin- and nanosilver-doped polymethylmethacrylate bone cement on primary human osteoclasts.
abstract: Bacterial colonization of implant surfaces is a feared complication in surgery and orthopedics. Due to the increasing number of periprosthetic infections caused by multidrug-resistant microorganisms, new antibacterial coatings for biomaterials must be developed. The excellent antibacterial properties of silver nanoparticles (AgNPs) against multidrug-resistant bacteria, for example, have been repeatedly described. For this reason, we tested a nanosilver-doped polymethylmethacrylate (PMMA) bone cement and a nanosilver-coated titanium alloy regarding their influence on osteoclastogenesis of primary human peripheral blood mononuclear cells. Both implant variants did not inhibit osteoclast differentiation. Excellent cell attachment and unaltered podosomal structures were confirmed. Additionally, no induction of oxidative or endoplasmic reticulum stress could be observed. However, PMMA loaded with gentamicin and nanosilver inhibited preosteoclast fusion and further osteoclastogenesis. The material also led to decreased clathrin-dependent endocytosis as well as decreased levels of endoplasmic reticulum stress. Therefore, biomaterial functionalization with AgNPs did not disturb osteoclastogenesis, while addition of gentamicin reduced the cytocompatibility of nanosilver-doped materials towards human osteoclasts.
authors: Laboratory for Experimental Trauma Surgery, Justus Liebig University Giessen, Giessen, Germany.
authors: Linda
authors: L
authors: Pauksch
authors: Jörg
authors: J
authors: Franke
authors: Reinhard
authors: R
authors: Schnettler
authors: Katrin S
authors: KS
authors: Lips
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001843:Bone Cements / Q000737:chemistry*; D002454:Cell Differentiation; D004357:Drug Synergism; D005839:Gentamicins / Q000633:toxicity*; D006801:Humans; D053758:Nanoparticles; D010010:Osteoclasts / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D001843:Bone Cements; D005839:Gentamicins; D019904:Polymethyl Methacrylate