title: Controlling the blood glucose of type 1 diabetes mice by co-culturing MIN-6 β cells on 3D scaffold.
abstract: T1D is an autoimmune disease, which may be caused by lack of insulin-secreting β cells due to damage of autoimmune system. Living with T1D is a challenge for the child and the family; cell transplantation is a treatment option for diabetes in children. To establish a microenvironment suitable for cell growth and proliferation as well as for sustained cellular function, we used MIN-6 β cells as seed cells and SF-IV collagen as a 3D composite scaffold to construct artificial pancreas in this experiment. The cell viabilities were determined by MTT assay, and the response of cells to different glucose concentrations was observed by glucose stimulation test. Artificial pancreas was transplanted into the abdominal cavity of T1D mice, and the changes of blood glucose were monitored. After 10 days, insulin expression was detected by immunohistochemical method, and the claybank stained area showed effectiveness of insulin secretion. A series of experiments showed that implantation of 3D cell scaffold into the abdominal cavity can effectively control the blood glucose level of T1D mice. It also had longer-lasting hypoglycemic effects than simple cell transplantation, which was expected to become a new method for the treatment of T1D.
authors: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China; Department of General Surgery, Tenth People's Hospital of Tongji University, Shanghai, China.
authors: Xiaoqi
authors: X
authors: Yuan
authors: Yan
authors: Y
authors: Huang
authors: Yibing
authors: Y
authors: Guo
authors: Lei
authors: L
authors: Wang
authors: Qingsong
authors: Q
authors: Guo
authors: Tianxing
authors: T
authors: Xu
authors: Di
authors: D
authors: Wu
authors: Pengcheng
authors: P
authors: Zhou
authors: Shajun
authors: S
authors: Zhu
authors: Yao
authors: Y
authors: Wang
authors: Xiangjun
authors: X
authors: Fan
authors: Mingyan
authors: M
authors: Zhu
authors: Yuhua
authors: Y
authors: Lu
authors: Zhiwei
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D001786:Blood Glucose; D007328:Insulin
keywords: 3D scaffold; MIN-6 β cells; blood glucose; co-cultured; silk fibroin-IV collagen; type 1 diabetes