title: How do eggs get fat? Insights into ovarian fatty acid accumulation in the shortfinned eel, Anguilla australis.
abstract: Previous research using eels has shown that 11-ketotestosterone can induce ovarian triacylglyceride accumulation both in vivo and in vitro. Further, accumulation is dramatically enhanced in the presence of very-low density lipoprotein. This study examined the involvement of the low density lipoprotein receptor and vitellogenin receptor in oocyte lipid accumulation. Specific antisera were used in an attempt to block the vitellogenin receptor and/or the low density lipoprotein receptor. Accordingly, incubation with the low density lipoprotein receptor antiserum clearly reduced the oocyte diameter and the amount of oil present within the oocyte. In contrast, blocking the vitellogenin receptor had little effect on either oocyte surface area or the abundance of oil droplets in the cytosol. In keeping with birds, we conclude that the low density lipoprotein receptor is a major player involved in mediating ovarian fatty acid accumulation in the eel. However, lipoprotein lipase-mediated fatty acid accumulation also remains conceivable, for example through interactions between this enzyme and the low density lipoprotein receptor.
authors: Department of Zoology, University of Otago, 340 Great King Street, PO Box 56, Dunedin 9054, New Zealand. Electronic address: erin.damsteegt@otago.ac.nz.
authors: Erin L
authors: EL
authors: Damsteegt
authors: Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors: Hiroko
authors: H
authors: Mizuta
authors: Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.
authors: Naoshi
authors: N
authors: Hiramatsu
authors: Department of Zoology, University of Otago, 340 Great King Street, PO Box 56, Dunedin 9054, New Zealand.
authors: P Mark
authors: PM
authors: Lokman
mesh_terms: D000811:Anguilla / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D004527:Egg Proteins / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D005227:Fatty Acids; D008079:Lipoproteins, VLDL; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor; D008071:Lipoprotein Lipase
keywords: Fatty acid; Lipoprotein lipase; Low density lipoprotein; Low density lipoprotein receptor; Oil droplet; Very-low density lipoprotein