title: N-Glycosylation with synthetic undecaprenyl pyrophosphate-linked oligosaccharide to oligopeptides by PglB oligosaccharyltransferase from Campylobacter jejuni.
abstract: The oligosaccharyltransferase PglB from Campylobacter jejuni catalyses the N-glycosylation reaction with undecaprenyl-pyrophosphate-linked Glc1 GalNAc5 Bac1 (Und-PP-Glc1 GalNAc5 Bac1 ). Experiments using chemically synthesized donors coupled to fluorescently tagged peptides confirmed that biosynthetic intermediate Und-PP-Bac1 and Und-PP-GalNAc2 Bac1 are transferred efficiently to the Asn residue in the consensus sequence (D/E-X'-N-X-T/S, X',X≠P). The products were analyzed in detail by tandem MS to confirm their chemical structures.
authors: RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan). aishiwa@riken.jp.
authors: Akihiro
authors: A
authors: Ishiwata
authors: Yuya
authors: Y
authors: Taguchi
authors: Yong Joo
authors: YJ
authors: Lee
authors: Taisuke
authors: T
authors: Watanabe
authors: Daisuke
authors: D
authors: Kohda
authors: Yukishige
authors: Y
authors: Ito
mesh_terms: D016123:Campylobacter jejuni / Q000201:enzymology*; D006031:Glycosylation; D006602:Hexosyltransferases / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D009842:Oligopeptides; D009844:Oligosaccharides; D011106:Polyisoprenyl Phosphates; C088139:undecaprenyl pyrophosphate; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase
keywords: glycopeptides; oligosaccharides; oligosaccharyltransferases; substrate specificity; undecaprenyl pyrophosphate-linked glycan