title: Characterization of oxidative phosphorylation enzymes in Euglena gracilis and its white mutant strain W(gm)ZOflL.
abstract: The enzymes involved in Euglena oxidative phosphorylation (OXPHOS) were characterized in this study. We have demonstrated that Euglena gracilis strain Z and its stable bleached non-photosynthetic mutant strain WgmZOflL both possess fully functional OXPHOS apparatus as well as pathways requiring terminal alternative oxidase(s) and alternative mitochondrial NADH-dehydrogenase(s). Light (or dark) and plastid (non)functionality seem to have little effect on oxygen consumption, the activities of the enzymes involved in OXPHOS and the action of respiration inhibitors in Euglena. This study also demonstrates biochemical properties of complex III (cytochrome c reductase) in Euglena.
authors: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
authors: Katarína
authors: K
authors: Krnáčová
authors: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
authors: Ivana
authors: I
authors: Rýdlová
authors: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
authors: Michaela
authors: M
authors: Vinarčíková
authors: Department of Genetics, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia.
authors: Juraj
authors: J
authors: Krajčovič
authors: Department of Biology and Ecology, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
authors: Matej
authors: M
authors: Vesteg
authors: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia. Electronic address: horvath@fns.uniba.sk.
authors: Anton
authors: A
authors: Horváth
mesh_terms: D045222:Electron Transport Chain Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D005056:Euglena gracilis / Q000201:enzymology* / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology; D009154:Mutation; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D045222:Electron Transport Chain Complex Proteins; D015800:Protozoan Proteins
keywords: Bleaching; Euglenozoa; Mitochondria; Respiratory chain; Trypanosomatids