title: Structure of the capsular polysaccharide of Acinetobacter baumannii 1053 having the KL91 capsule biosynthesis gene locus.
abstract: Acinetobacter baumannii 1053 is the type strain for the maintenance of specific bacteriophage AP22, which infects a fairly broad range of A. baumannii strains circulating in Russian clinics and hospitals. A capsular polysaccharide (CPS) was isolated from cells of strain 1053 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: -->4)-β-D-ManpNAcA-(1-->4)-β-D-ManpNAcA-(1-->3)-α-D-FucpNAc-(1--> where ManNAcA and FucNAc indicate 2-acetamido-2-deoxymannuronic acid and 2-acetamido-2,6-dideoxygalactose, respectively. A polysaccharide having the same repeating unit but a shorter chain was isolated by the phenol-water extraction of bacterial cells. Sequencing of the CPS biosynthesis gene locus showed that A. baumannii 1053 belongs to a new group designated KL91. The gene functions assigned putatively by a comparison with available databases were in agreement with the CPS structure established.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Alexander S
authors: AS
authors: Shashkov
authors: M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Mikhail M
authors: MM
authors: Shneider
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Sof'ya N
authors: SN
authors: Senchenkova
authors: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors: Anastasiya V
authors: AV
authors: Popova
authors: Research Institute of Physical-Chemical Medicine, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia.
authors: Anastasia S
authors: AS
authors: Nikitina
authors: Research Institute of Physical-Chemical Medicine, Moscow, Russia.
authors: Vladislav V
authors: VV
authors: Babenko
authors: Research Institute of Physical-Chemical Medicine, Moscow, Russia; Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia.
authors: Elena S
authors: ES
authors: Kostryukova
authors: M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Konstantin A
authors: KA
authors: Miroshnikov
authors: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors: Nikolay V
authors: NV
authors: Volozhantsev
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial
keywords: 2-Acetamido-2,6-dideoxy-d-galactose; 2-Acetamido-2-deoxy-d-mannuronic acid; Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide biosynthesis gene locus