title: Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: Lipid enhancement methods and media optimization using response surface methodology.
abstract: Two fresh water microalgae, Coelastrella sp. M-60 and Micractinium sp. M-13 were investigated in this study for their potential of biodiesel production. For increasing biomass and lipid production, these microalgae were subjected to nutrient starvation (nitrogen, phosphorous, iron), salinity stress and nutrient supplementation with sugarcane industry effluent, citric acid, glucose and vitamin B12. The lipid productivity obtained from the isolates Coelastrella sp. M-60 (13.9 ± 0.4 mg/L/day) and Micractinium sp. M-13 (11.1 ± 0.2 mg/L/day) was maximum in salinity stress. The media supplemented with all the four nutrients yielded higher lipid productivity than the control. The response surface methodology (RSM) was employed to evaluate the effect of sugarcane industry effluent and citric acid on growth and lipid yield. Fatty acid profile of Coelastrella sp. M-60 and Micractinium sp. M-13 were composed of C-14, C-16:0, C-18:0, C-18:1 and C-18:2 and their fuel properties were also in accordance with international standards.
authors: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors: Rathinasamy
authors: R
authors: Karpagam
authors: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors: Kalimuthu Jawahar
authors: KJ
authors: Raj
authors: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors: Balasubramaniem
authors: B
authors: Ashokkumar
authors: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India. Electronic address: vara5277@gmail.com.
authors: Perumal
authors: P
authors: Varalakshmi
mesh_terms: D056804:Biofuels; D018533:Biomass; D002855:Chromatography, Thin Layer; D019343:Citric Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005618:Fresh Water; D005742:Gasoline; D005947:Glucose / Q000737:chemistry; D007218:Industrial Microbiology; D007501:Iron / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D009995:Osmosis; D010758:Phosphorus / Q000737:chemistry; D054712:Salinity; D014805:Vitamin B 12 / Q000737:chemistry
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D005742:Gasoline; D008055:Lipids; D010758:Phosphorus; D019343:Citric Acid; D007501:Iron; D005947:Glucose; D009584:Nitrogen; D014805:Vitamin B 12
keywords: Biodiesel; Coelastrella; Lipid enhancement; Micractinium; Salinity stress