title: In vitro evaluation of biocompatibility of Ti-Mo-Sn-Zr superelastic alloy.
abstract: Shape memory alloys (SMAs) including superelastic alloys have unique properties such as shape memory and superelasticity, thus they are recognized as very useful biomaterials. These properties are very advantageous for medical use, and actually the SMA wires have been widely used in medical field. However, biocompatibility of nickel-titanium (Ni-Ti) alloy, which is the only practical SMA at present, has been questioned because of its high nickel content. The aim of this study was to evaluate the biocompatibility of a newly developed Ni-free Ti-based SMA for medical use. The newly developed SMA made of Ti-Mo-Sn-Zr system was processed into a disk of 15.1 mm in diameter. Pure titanium of the same shape was prepared as control. All the disk surfaces were polished using emery papers, #120, #400, and #600. Scanning electron microscopy and a 3D optics profiler were used to evaluate the surface of the materials. In vitro evaluations included colony examination for evaluation of the cell cytotoxicity, DNA quantification for the cell proliferation, Alamar blue assay for metabolic activity, FDA staining for the live cell imaging, and cell cycle analysis, using Chinese hamster fibroblastic V-79 cells and mouse osteoblastic MC3T3-E1 cells. In colony examination and DNA quantification, there was no significant difference between the Ti-Mo-Sn-Zr and the pure titanium. In FDA staining, cultured cells on the Ti-Mo-Sn-Zr alloy showed the same biocompatibility as those on the pure titanium. The present results suggest that the newly developed Ti-Mo-Sn-Zr alloy showed the high biocompatibility comparable to pure titanium and can be used as efficient biomaterial for medical use.
authors: Division of Oral Dysfunction Science, Graduate School of Density, Tohoku University, Sendai, Japan.
authors: Shoko
authors: S
authors: Nunome
authors: Liaison Center for Innovative Dentistry, Graduate School of Density, Tohoku University, Sendai, Japan Division Biomedical Engineering for Diagnosis and Treatment, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan kanetaka@dent.tohoku.ac.jp.
authors: Hiroyasu
authors: H
authors: Kanetaka
authors: Division of Oral Physiology, Graduate School of Density, Tohoku University, Sendai, Japan.
authors: Tada-aki
authors: TA
authors: Kudo
authors: Precision and Intelligence Laboratory, Tokyo Institute of Technology, Yokohama, Japan.
authors: Kazuki
authors: K
authors: Endoh
authors: Precision and Intelligence Laboratory, Tokyo Institute of Technology, Yokohama, Japan.
authors: Hideki
authors: H
authors: Hosoda
authors: Division of Oral Dysfunction Science, Graduate School of Density, Tohoku University, Sendai, Japan.
authors: Kaoru
authors: K
authors: Igarashi
mesh_terms: D000497:Alloys / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004548:Elasticity; D051379:Mice; D008982:Molybdenum / Q000737:chemistry* / Q000633:toxicity; D013314:Stress, Mechanical; D014001:Tin / Q000737:chemistry* / Q000633:toxicity; D014025:Titanium / Q000737:chemistry* / Q000633:toxicity; D015040:Zirconium / Q000737:chemistry* / Q000633:toxicity
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D014001:Tin; D008982:Molybdenum; D004247:DNA; D015040:Zirconium; D014025:Titanium
keywords: Biocompatibility; Ti–Mo–Sn–Zr alloy; in vitro evaluation; shape memory alloy; superelasticity