title: Interaction of peroxiredoxin V with dihydrolipoamide branched chain transacylase E2 (DBT) in mouse kidney under hypoxia.
abstract: BACKGROUND
  Peroxiredoxin V (Prdx V) plays a major role in preventing oxidative damage as an effective antioxidant protein within a variety of cells through peroxidase activity. However, the function of Prdx V is not limited to peroxidase enzymatic activity per se. It appears to have unique function in regulating cellular response to external stimuli by directing interaction with signaling protein. In this study, we identified Prdx V interacting partners in mouse kidney under hypoxic stress using immunoprecipitation and shotgun proteomic analysis (LC-MS/MS).
  
  
  RESULTS
  Immunoprecipitation coupled with nano-UPLC-MS(E) shotgun proteomics was employed to identify putative interacting partners of Prdx V in mouse kidney in the setting of hypoxia. A total of 17 proteins were identified as potential interacting partners of Prdx V by a comparative interactomics analysis in kidney under normoxia versus hypoxia. Dihydrolipoamide branched chain transacylase E2 (DBT) appeared to be a prominent candidate protein displaying enhanced interaction with Prdx V under hypoxic stress. Moreover, hypoxic kidney exhibited altered DBT enzymatic activity compared to normoxia. An enhanced colocalization of these two proteins under hypoxic stress was successfully observed in vitro. Furthermore, peroxidatic cysteine residue (Cys48) of Prdx V is likely to be responsible for interacting with DBT.
  
  
  CONCLUSIONS
  We identified several proteins interacting with Prdx V under hypoxic condition known to induce renal oxidative stress. In hypoxic condition, we observed an enhanced interaction of Prdx V and DBT protein as well as increased DBT enzymatic activity. The results from this study will contribute to enhance our understanding of Prdx V's role in hypoxic stress and may suggest new directions for future research.
authors: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 300 Yongbong-Dong, Buk-Ku, Gwangju, 500-757 Republic of Korea.
authors: Sun Hee
authors: SH
authors: Ahn
authors: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 300 Yongbong-Dong, Buk-Ku, Gwangju, 500-757 Republic of Korea.
authors: Hee-Young
authors: HY
authors: Yang
authors: Department of Molecular Medicine, Graduate School, Chonnam National University, Gwangju, Republic of Korea.
authors: Gia Buu
authors: GB
authors: Tran
authors: Korea Basic Science Institute, Daejeon, Republic of Korea.
authors: Joseph
authors: J
authors: Kwon
authors: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: Kyu-Yeol
authors: KY
authors: Son
authors: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 300 Yongbong-Dong, Buk-Ku, Gwangju, 500-757 Republic of Korea.
authors: Suhee
authors: S
authors: Kim
authors: Department of Molecular Medicine, Graduate School, Chonnam National University, Gwangju, Republic of Korea.
authors: Quoc Thuong
authors: QT
authors: Dinh
authors: Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors: Seunggon
authors: S
authors: Jung
authors: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: Ha-Mi
authors: HM
authors: Lee
authors: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors: Kyoung-Oh
authors: KO
authors: Cho
authors: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 300 Yongbong-Dong, Buk-Ku, Gwangju, 500-757 Republic of Korea.|Department of Molecular Medicine, Graduate School, Chonnam National University, Gwangju, Republic of Korea.
authors: Tae-Hoon
authors: TH
authors: Lee
keywords: Dihydrolipoamide branched-chain transacylase; Hypoxia; Kidney; Peroxidatic cysteine; Peroxiredoxin V