title: In vitro Anti-oxidant Activity and HPLC-DAD System Based Phenolic Content Analysis of Codiaeum variegatum Found in Bangladesh.
abstract: OBJECTIVE
  This study evaluated the in vitro antioxidant potential of two varieties of Codiaeum variegatum leaves (spiral (CP) and royal like (BP)) extracts.
  
  
  METHODS
  The different antioxidant assays, including DPPH free radical scavenging, nitric oxide scavenging, hydrogen peroxide, reducing power, total antioxidant activity, protection of lipid peroxidation and RBC membrane stabilization activity, were studied. Moreover, high-performance liquid chromatography (HPLC) coupled with diode-array detection was used to identify and quantify the phenolic compounds in the royal like (BP) leaves extract.
  
  
  RESULTS
  Codiaeum variegatum extracts showed effective DPPH free radical scavenging, hydrogen peroxide radical scavenging and nitric oxide scavenging activity. However, reducing power of ferric ion was not significant compared to the standard antioxidant activity. In addition, Codiaeum variegatum extracts exhibited protection against lipid peroxidation. The total antioxidant activity was increased dose dependently when compared with standard drug ascorbic acid. (-)-Epicatechin, p-coumaric acid, rutin hydrate and ellagic acid were identified in the extract. Among the phenolic compounds, ellagic acid was abundantly present in the extract.
  
  
  CONCLUSIONS
  Our investigation suggests that Codiaeum variegatum leaves contain high amount of phenolic compounds which may responsible for its biological activities in folkloric medicine.
authors: Department of Pharmacy, Stamford University, Dhaka, Bangladesh.
authors: Nadia
authors: N
authors: Saffoon
authors: Department of Pharmacy, Stamford University, Dhaka, Bangladesh.
authors: Riaz
authors: R
authors: Uddin
authors: School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, Australia.
authors: Nusrat
authors: N
authors: Subhan
authors: BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh.
authors: Hemayet
authors: H
authors: Hossain
authors: Department of Pharmaceutical Sciences, North South University, Bangladesh.
authors: Hasan Mahmud
authors: HM
authors: Reza
authors: Department of Pharmaceutical Sciences, North South University, Bangladesh.
authors: Md Ashraful
authors: MA
authors: Alam
keywords: Codiaeum variegatum; DPPH; Ellagic acid; Hydrogen peroxide; Membrane stabilizing activity; Nitric oxide