title: Noncovalently functionalized monolayer graphene for sensitivity enhancement of surface plasmon resonance immunosensors.
abstract: A highly efficient surface plasmon resonance (SPR) immunosensor is described using a functionalized single graphene layer on a thin gold film. The aim of this approach was two-fold: first, to amplify the SPR signal by growing graphene through chemical vapor deposition and, second, to control the immobilization of biotinylated cholera toxin antigen on copper coordinated nitrilotriacetic acid (NTA) using graphene as an ultrathin layer. The NTA groups were attached to graphene via pyrene derivatives implying π-π interactions. With this setup, an immunosensor for the specific antibody anticholera toxin with a detection limit of 4 pg mL(-1) was obtained. In parallel, NTA polypyrrole films of different thicknesses were electrogenerated on the gold sensing platform where the optimal electropolymerization conditions were determined. For this optimized polypyrrole-NTA setup, the simple presence of a graphene layer between the gold and polymer film led to a significant increase of the SPR signal.
authors: Département de Chimie Moléculaire, Univ. Grenoble Alpes, CNRS , UMR 5250, F-38000 Grenoble, France.
authors: Meenakshi
authors: M
authors: Singh
authors: Michael
authors: M
authors: Holzinger
authors: Maryam
authors: M
authors: Tabrizian
authors: Sinéad
authors: S
authors: Winters
authors: Nina C
authors: NC
authors: Berner
authors: Serge
authors: S
authors: Cosnier
authors: Georg S
authors: GS
authors: Duesberg
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D019921:Biotinylation; D002772:Cholera Toxin / Q000276:immunology; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D020349:Surface Plasmon Resonance / Q000379:methods*; D014835:Volatilization
chemical_list: D055910:Antibodies, Immobilized; D006046:Gold; D006108:Graphite; D002772:Cholera Toxin