title: Structural characteristics of ScBx genes controlling the biosynthesis of hydroxamic acids in rye (Secale cereale L.).
abstract: Benzoxazinoids (BX) are major secondary metabolites of gramineous plants that play an important role in disease resistance and allelopathy. They also have many other unique properties including anti-bacterial and anti-fungal activity, and the ability to reduce alfa-amylase activity. The biosynthesis and modification of BX are controlled by the genes Bx1 ÷ Bx10, GT and glu, and the majority of these Bx genes have been mapped in maize, wheat and rye. However, the genetic basis of BX biosynthesis remains largely uncharacterized apart from some data from maize and wheat. The aim of this study was to isolate, sequence and characterize five genes (ScBx1, ScBx2, ScBx3, ScBx4 and ScBx5) encoding enzymes involved in the synthesis of DIBOA, an important defense compound of rye. Using a modified 3D procedure of BAC library screening, seven BAC clones containing all of the ScBx genes were isolated and sequenced. Bioinformatic analyses of the resulting contigs were used to examine the structure and other features of these genes, including their promoters, introns and 3'UTRs. Comparative analysis showed that the ScBx genes are similar to those of other Poaceae species, especially to the TaBx genes. The polymorphisms present both in the coding sequences and non-coding regions of ScBx in relation to other Bx genes are predicted to have an impact on the expression, structure and properties of the encoded proteins.
authors: Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, 159 Nowoursynowska Str, 02-776, Warsaw, Poland, beata_bakera@sggw.pl.
authors: Beata
authors: B
authors: Bakera
authors: Bogna
authors: B
authors: Makowska
authors: Jolanta
authors: J
authors: Groszyk
authors: Michał
authors: M
authors: Niziołek
authors: Wacław
authors: W
authors: Orczyk
authors: Hanna
authors: H
authors: Bolibok-Brągoszewska
authors: Aneta
authors: A
authors: Hromada-Judycka
authors: Monika
authors: M
authors: Rakoczy-Trojanowska
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D019295:Computational Biology; D018744:DNA, Plant / Q000235:genetics; D005091:Exons; D015723:Gene Library; D017343:Genes, Plant; D006877:Hydroxamic Acids / Q000737:chemistry*; D007438:Introns; D011401:Promoter Regions, Genetic; D012434:Secale / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D006877:Hydroxamic Acids