title: [New approach for JC virus detection and its application for PML diagnosis].
abstract: Progressive multifocal leukoencephalopathy (PML) is a rare but fatal demyelinating disease of the central nervous system (CNS) caused by JC virus (JCV). The current diagnostic standard for PML is real-time PCR testing of extracted DNA for assessing the presence of JCV DNA in cerebrospinal fluid (CSF). However, because of its sensitivity, real-time PCR assay for JCV testing has a risk of false-positive results due to DNA contamination. JCV isolates recovered from the brain or CSF of PML patients contain hypervariable mutations within the non-coding control region (NCCR) of the viral genome. In our laboratory, the high-resolution melting (HRM) assay was developed to distinguish the patient-dependent NCCR patterns of JCV DNA variants in clinical specimens. The HRM-based scanning of NCCR serves as a quick and convenient technique for comparing the mutational patterns of JCV variants in clinical samples and for the confirmation of PML diagnosis when combined with routine real-time PCR testing.
authors: Department of Virology 1, National Institute of Infectious Diseases.
authors: Kazuo
authors: K
authors: Nakamichi
authors: Chang-Kweng
authors: CK
authors: Lim
authors: Masayuki
authors: M
authors: Saijo
mesh_terms: D004279:DNA, Viral / Q000134:cerebrospinal fluid*; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000175:diagnosis* / Q000821:virology*; D009154:Mutation; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral