title: Activation of RAW264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms.
abstract: The polysaccharide from the roots of Actinidia eriantha (AEPS), a potent antitumor agent and immunological adjuvant, was investigated for the immunomodulatory effects on RAW264.7 macrophages and its molecular mechanisms. AEPS could significantly enhance the pinocytic and phagocytic activity, induce the production of NO, TNF-α, IL-10, IL-1β and IL-6, and promote the expression of accessory and costimulatory molecules in RAW264.7 cells. PCR array assay revealed that AEPS up-regulated 28 genes including TLRs (TLR2, TLR8, TLR9), proinflammatory factors (IL-1β, G-CSF, IL-1α, GM-CSF, IL-6, COX-2, TNF-α, IFN-β, CXCL10, CCL2, TNF-β, IL-10), and the genes involved in NF-κB signaling pathway, and down-regulated 6 genes such as TLR3, TLR4, PGLYRP1, EIF2αK2, MAP3K1 and IRF1. AEPS was further showed to promote cytoplasmic IκB-α degradation and increase nuclear NF-κB p65 levels in RAW264.7 cells. These results suggested that AEPS activated RAW264.7 macrophages and elicited a M1 and M2 response through TLRs/NF-κB signaling pathway.
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: sunhx@zju.edu.cn.
authors: Hongxiang
authors: H
authors: Sun
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Juan
authors: J
authors: Zhang
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
authors: Fengyang
authors: F
authors: Chen
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Xiangfeng
authors: X
authors: Chen
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Zhihua
authors: Z
authors: Zhou
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Hui
authors: H
authors: Wang
mesh_terms: D029042:Actinidia / Q000737:chemistry*; D000818:Animals; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D011134:Polysaccharides; D051193:Toll-Like Receptors
keywords: Actinidia eriantha; NF-κB signaling pathway; PCR array; Polysaccharides; RAW264.7 cell; Toll-like receptor (TLR)