title: Degradation pathway and kinetic analysis for p-xylene removal by a novel Pandoraea sp. strain WL1 and its application in a biotrickling filter.
abstract: In this study, a novel Pandoraea sp. strain WL1 capable of mineralizing p-xylene as sole carbon and energy source was isolated from the activated sludge of a pharmaceutical wastewater treatment plant. A nearly complete degradation of 16.6∼99.4 mg L(-1)p-xylene in the liquid-phase was achieved within 6∼18 h accompanied by 15.9∼56.3 mg dry cell weight (DCW)L(-1) for bacterial growth. A complete pathway for p-xylene degradation by strain WL1 was presented through identification of a major intermediate (p-toluic acid) and final products (2.193 gCO2 gp-xylene(-1) of CO₂ production and 0.215 g DCW gp-xylene(-1) of bacterial yield). Kinetics of bacterial growth and p-xylene degradation were evaluated using Haldane-Andrews model and pseudo first-order model, respectively. Furthermore, a biotrickling filter (BTF) was employed to evaluate the application of strain WL1 on the removal of gas-phase p-xylene under gas flow rates of 0.41∼1.98 m(3)h(-1) for inlet loading rates of 5∼248 gm(-3)h(-1). The BTF inoculated with strain WL1 proved to be robust against fluctuations of gas flow rates and inlet p-xylene concentrations. All the results obtained highlight the potential of strain WL1 for the treatment of p-xylene.
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Institute of Industrial Ecology and Environment, College of Chemical and Biological Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, China; Institute of Environmental Engineering, Zhejiang University (Zijingang Campus), Hangzhou 310058, China.
authors: Xiangqian
authors: X
authors: Wang
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Institute of Industrial Ecology and Environment, College of Chemical and Biological Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, China; Institute of Environmental Engineering, Zhejiang University (Zijingang Campus), Hangzhou 310058, China.
authors: Qiaoli
authors: Q
authors: Wang
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Institute of Industrial Ecology and Environment, College of Chemical and Biological Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, China.
authors: Sujing
authors: S
authors: Li
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Institute of Industrial Ecology and Environment, College of Chemical and Biological Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, China; Institute of Environmental Engineering, Zhejiang University (Zijingang Campus), Hangzhou 310058, China. Electronic address: w_li@zju.edu.cn.
authors: Wei
authors: W
authors: Li
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology; D042521:Burkholderiaceae / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D002245:Carbon Dioxide / Q000737:chemistry; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005374:Filtration; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D014877:Water Pollution, Chemical / Q000032:analysis*; D014992:Xylenes / Q000737:chemistry*
chemical_list: D014874:Water Pollutants, Chemical; D014992:Xylenes; D002245:Carbon Dioxide; C031286:4-xylene
keywords: Biotrickling filter; Degradation pathway; Kinetic analysis; Pandoraea sp. WL1; p-Xylene