title: MiR-144 inhibits proliferation and induces apoptosis and autophagy in lung cancer cells by targeting TIGAR.
abstract: BACKGROUND
  MiRNAs are noncoding RNAs of 20-24 nucleotides that function as post-transcriptional negative regulators of gene expression. MiRNA genes are usually transcribed by RNA polymerase II in the nucleus. Their initial products are pre-miRNAs which have cap sequences and polyA tails. The p53-induced glycolysis and apoptosis regulator (TIGAR) was discovered through microarray analysis of gene expression following activation of p53. However, little is known about the effect of miR-144 on cell proliferation and apoptosis and how it interacts with TIGAR.
  
  
  METHODS
  We performed real-time PCR, western blotting, CCK8, colony formation, tumor growth, flow cytometry, Caspase3/7 activity, Hoechst 33342 staining, MDC staining of autophagic cells and luciferase reporter assays to detect the influence of miR-144 to lung cancer cells.
  
  
  RESULTS
  miR-144 targeted TIGAR, inhibited proliferation, enhanced apoptosis, and increased autophagy in A549 and H460 cells.
  
  
  CONCLUSIONS
  Our study improves our understanding of the mechanisms underlying lung cancer pathogenesis and may promote the development of novel targeted therapies.
authors: Department of Respiratory Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors: Shanshan
authors: S
authors: Chen
authors: Ping
authors: P
authors: Li
authors: Juan
authors: J
authors: Li
authors: Yuanyuan
authors: Y
authors: Wang
authors: Yuwen
authors: Y
authors: Du
authors: Xiaonan
authors: X
authors: Chen
authors: Wenqiao
authors: W
authors: Zang
authors: Huaqi
authors: H
authors: Wang
authors: Heying
authors: H
authors: Chu
authors: Guoqiang
authors: G
authors: Zhao
authors: Guojun
authors: G
authors: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins; D001343:Autophagy / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D010744:Phosphoric Monoester Hydrolases
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D010744:Phosphoric Monoester Hydrolases; C512708:TIGAR protein, human