title: Spectroscopic investigation on the sonodynamic damage to protein in the presence of eosine B.
abstract: In this work, bovine serum albumin (BSA) and eosine B (EB) were selected as a model protein and sonosensitizer, respectively. The sonodynamic damage to protein in the presence of EB and its mechanism were studied by means of absorption and fluorescence spectra. The results indicated that the synergistic effects of ultrasound and EB can efficiently damage the BSA molecules, and the damage of protein could be mainly due to the generation of reactive oxygen species (ROS). The damage degree of protein increased with the increase of ultrasonic time and EB concentration because of the increased quantities of ROS. Hydroxyl free radical (OH) was the major mediators of the ultrasound-inducing proteins damage in the presence of EB. In addition, the quantities of ROS from the diphenylcarbazide (DPCI)-EB solutions and the DPCI-fluorescein (FS) solutions with or without ROS scavengers were contrasted, respectively. The results show that FS mainly produce OH, but the quantities of ROS in the presence of FS were lower than those of EB, which indicates that the nitro and bromine substituent groups on the benzene ring of FS increase the quantity of ROS, but do not change the kinds of ROS.
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Ling-Ling
authors: LL
authors: He
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Xiao-Xia
authors: XX
authors: Wu
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Yong-Xia
authors: YX
authors: Wang
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Xian-Ping
authors: XP
authors: Liu
authors: College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Yan-Ling
authors: YL
authors: Song
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Yan-Jie
authors: YJ
authors: Yang
authors: College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors: Yan-Ping
authors: YP
authors: Gao
authors: School of Pharmaceutical Sciences, Liaoning University, Shenyang 110036, China.
authors: Xin
authors: X
authors: Wang
authors: School of Pharmaceutical Sciences, Liaoning University, Shenyang 110036, China.
authors: Bin
authors: B
authors: Liu
authors: School of Pharmaceutical Sciences, Liaoning University, Shenyang 110036, China. Electronic address: xin_wang@lnu.edu.cn.
authors: Xin
authors: X
authors: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D010697:Eosine I Bluish / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D000069453:Ultrasonic Waves
chemical_list: D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D010697:Eosine I Bluish
keywords: Bovine serum albumin (BSA); Eosine B; Reactive oxygen species (ROS); Sonodynamic damage; Spectroscopy