title: Effects of ovarian hyperstimulation on mitochondria in oocytes and early embryos.
abstract: A mouse model was used to compare the number and function of mitochondria in oocytes and embryos obtained by superovulation and in a natural cycle (control group). The superovulation group had a higher number of total oocytes, MII oocytes, embryos with two pronuclei, 2-cell embryos and blastocysts than the control group (P < 0.05 for all). The superovulation group had high proportion of MII oocytes with low number of mitochondrial (mt) DNA copies. The average number of mtDNA copies, ATP level and mitochondrial membrane potential (??m) in MII oocytes in the superovulation were lower than in the control group (P < 0.05 for all). However, at the blastocyst stage, mean mtDNA copies, ATP level and ??m did not differ significantly between the two groups. These results suggest that ovarian hyperstimulation does not cause damage to the mitochondria in eggs but, rather, more eggs with poor mitochondrial quality are recruited, resulting in a decline in average mitochondrial quality.
authors: Wenzhou Medical University, Wenzhou 325035, PR China.|Department of Reproductive Endocrinology, Zhejiang Provincial People's Hospital, No. 158, Shangtang Road, Hangzhou 310014, PR China.
authors: Jing
authors: J
authors: Shu
authors: Department of Reproductive Endocrinology, Zhejiang Provincial People's Hospital, No. 158, Shangtang Road, Hangzhou 310014, PR China.|The Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou 310058, PR China.
authors: Li-Li
authors: LL
authors: Xing
authors: The Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou 310058, PR China.|International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, 910# Hengshan Road, Xuhui Area, Shanghai, PR China.
authors: Guo-Lian
authors: GL
authors: Ding
authors: The Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou 310058, PR China.|International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, 910# Hengshan Road, Xuhui Area, Shanghai, PR China.
authors: Xin-Mei
authors: XM
authors: Liu
authors: Institute of Genetics, College of Life Science, Zhejiang University, Hangzhou 310058, PR China.
authors: Qing-Feng
authors: QF
authors: Yan
authors: The Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou 310058, PR China.|International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, 910# Hengshan Road, Xuhui Area, Shanghai, PR China.|Corresponding author. Email: huanghefg@hotmail.com.
authors: He-Feng
authors: HF
authors: Huang
keywords: mitochondrial DNA; mitochondrial membrane potential