title: Sustained release of vancomycin from novel biodegradable nanofiber-loaded vascular prosthetic grafts: in vitro and in vivo study.
abstract: This study describes novel biodegradable, drug-eluting nanofiber-loaded vascular prosthetic grafts that provide local and sustained delivery of vancomycin to surrounding tissues. Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol. The solution was then electrospun into nanofibers onto the surface of vascular prostheses. The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay. Experimental results indicated that the drug-eluting prosthetic grafts released high concentrations of vancomycin in vitro (well above the minimum inhibitory concentration) for more than 30 days. In addition, the in vivo release behavior of the drug-eluting grafts implanted in the subcutaneous pocket of rabbits was also documented. The drug-eluting grafts developed in this work have potential applications in assisting the treatment of vascular prosthesis infection and resisting reinfection when an infected graft is to be exchanged.
authors: Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors: Kuo-Sheng
authors: KS
authors: Liu
authors: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors: Cheng-Hung
authors: CH
authors: Lee
authors: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors: Yi-Chuan
authors: YC
authors: Wang
authors: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors: Shih-Jung
authors: SJ
authors: Liu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001807:Blood Vessel Prosthesis; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D054855:Drug-Eluting Stents; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry*; D011474:Prosthesis Design; D011817:Rabbits; D014640:Vancomycin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D014640:Vancomycin
keywords: drug-eluting prosthetic graft; release characteristics; vascular prosthesis infection