title: Characterizations of four toll-like receptor 4s in grass carp Ctenopharyngodon idellus and their response to grass carp reovirus infection and lipopolysaccharide stimulation.
abstract: In this study, the subcellular localization, tissue distribution and response to grass carp reovirus (GCRV) infection and lipopolysaccharide (LPS) stimulation of four grass carp Ctenopharyngodon idellus toll-like receptor 4 (tlr4) genes were investigated. All four genes were constitutively expressed in all tissues studied, but the subcellular localization and tissue exhibiting the highest expression differed for each protein. Following GCRV infection, all the four tlr4s were upregulated in all tissues examined, and stimulation of C. idellus kidney (CIK) cells with LPS resulted in downregulation of all four tlr4s. These results provide a foundation for further investigation of tlr4 genes in bony fishes.
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; Graduated University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Y Y
authors: YY
authors: Pei
authors: R
authors: R
authors: Huang
authors: Y M
authors: YM
authors: Li
authors: L J
authors: LJ
authors: Liao
authors: Z Y
authors: ZY
authors: Zhu
authors: Y P
authors: YP
authors: Wang
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D008070:Lipopolysaccharides; D012087:Reoviridae; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4
keywords: gene expression; grass carp reovirus; subcellular localization; tissue distribution; virus infection