title: Green tea extract therapy diminishes hepatic fibrosis mediated by dual exposure to carbon tetrachloride and ethanol: A histopathological study.
abstract: The aim of the present study was to investigate the hepatoprotective effect of green tea extract (GTE) against the hepatic fibrosis induced by carbon tetrachloride (CCl4), ethanol, and dual exposure to CCl4plus ethanol in rats. In particular, an investigation of the three-dimensional architecture was conducted using scanning electron microscopy. Various techniques revealed that hepatic fibrosis with intermingled fibers was located between cells in the CCl4, ethanol and combined CCl4plus ethanol groups. The hepatic fibrosis differed among the ethanol, CCl4and CCl4plus ethanol groups in terms of the type, thickness and distribution of fibers. The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status. The rats were also found to regain normal body weight and fur color, which had earlier been discolored due to weight loss. The autopsy results also showed that the animal livers returned to the normal shape and color. GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal. GTE presents a safe therapeutic strategy for hepatic fibrosis.
authors: Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City 13060, Kuwait.
authors: Abdel-Majeed
authors: AM
authors: Safer
authors: Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City 13060, Kuwait.
authors: Mohamad
authors: M
authors: Afzal
authors: Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City 13060, Kuwait.
authors: Nomny
authors: N
authors: Hanafy
authors: The Pharmaceutical Research Institute at Albany College of Pharmacy and Health Sciences, Albany, NY 12208, USA ; College of Medicine, King Saud University, Riyadh 12372, Saudi Arabia.
authors: Shaker
authors: S
authors: Mousa
keywords: Masson’s trichrome stain; alanine aminotransferase; aspartate aminotransferase; carbon tetrachloride plus ethanol; dual exposure; extracellular matrix; green tea extract; scanning electron microscopy