title: The role of miR-182 in regulating pineal CLOCK expression after hypoxia-ischemia brain injury in neonatal rats.
abstract: Circadian rhythm disorder is a common neurological deficit caused by neonatal hypoxic-ischemic brain damage (HIBD). However, little is known about its underlying mechanisms. Our previous studies revealed a significant elevation of clock genes at the protein, but not mRNA, levels in the pineal gland after neonatal HIBD. To investigate the mechanisms of post-transcriptional regulation on clock genes, we screened changes of miRNA levels in the pineal gland after neonatal HIBD using high-throughput arrays. Within the miRNAs whose expression was significantly down-regulated, we identified one miRNA (miR182) that targeted the 3'-untranslated region (3'-UTR) of Clock, a key component of clock genes, and played a crucial role in regulating CLOCK expression after oxygen-glucose deprivation in primarily cultured pinealocytes. Our findings therefore provide new insight on studies of therapeutic targets for circadian rhythm disturbance after neonatal HIBD.
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Xin
authors: X
authors: Ding
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Bin
authors: B
authors: Sun
authors: Center for Circadian Clocks, Medical College, Soochow University, Suzhou 215003, PR China.
authors: Jian
authors: J
authors: Huang
authors: Department of Hematology and Oncology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Lixiao
authors: L
authors: Xu
authors: Department of Hematology and Oncology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Jian
authors: J
authors: Pan
authors: Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 225003, PR China.
authors: Chen
authors: C
authors: Fang
authors: Department of Hematology and Oncology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Yanfang
authors: Y
authors: Tao
authors: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
authors: Shukun
authors: S
authors: Hu
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Ronghu
authors: R
authors: Li
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Xing
authors: X
authors: Han
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Po
authors: P
authors: Miao
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Ying
authors: Y
authors: Wang
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China.
authors: Jian
authors: J
authors: Yu
authors: Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, PR China. Electronic address: xing_feng66@suda.edu.cn.
authors: Xing
authors: X
authors: Feng
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D000831:Animals, Newborn; D056926:CLOCK Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D015536:Down-Regulation; D005947:Glucose / Q000172:deficiency; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D010870:Pineal Gland / Q000378:metabolism*; D051381:Rats
chemical_list: D020413:3' Untranslated Regions; C577631:MIRN182 microRNA, rat; D035683:MicroRNAs; D056926:CLOCK Proteins; C538945:Clock protein, rat; D005947:Glucose; D010100:Oxygen
keywords: CLOCK; Clock genes; HIBD; Pineal gland; Post-transcriptional control; miR-182