title: A novel pyrazolone-based derivative induces apoptosis in human esophageal cells via reactive oxygen species (ROS) generation and caspase-dependent mitochondria-mediated pathway.
abstract: Pyrazolone complexes have strong bio-activity but the anti-tumor mechanism of pyrazolone-based metal complexes is not fully understood. In this study, the inhibitory effect and possible mechanism of a novel pyrazolone-based derivative compound (Cd-PMPP-SAL) on human esophageal cancer cells were investigated. We found that Cd-PMPP-SAL inhibited the proliferation of Eca-109 cells in a dose-dependent manner and induced the apoptosis in the cells. Interestingly, Cd-PMPP-SAL promoted the production of ROS, loss of mitochondrial membrane potential, PARP cleavage and activation of caspase-3/9. These results suggest Cd-PMPP-SAL-induced apoptosis might be mediated by the increased production of ROS and caspase-dependent mitochondria-mediated pathway. These results suggest that Cd-PMPP-SAL is a potential candidate for the treatment of esophageal cancer.
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China.
authors: Jing
authors: J
authors: Zhao
authors: Institute of Applied Chemistry, Xinjiang University, Urumqi 830046, PR China.
authors: Li
authors: L
authors: Zhang
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China.
authors: Jinyao
authors: J
authors: Li
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China.
authors: Ting
authors: T
authors: Wu
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China.
authors: Meifang
authors: M
authors: Wang
authors: Institute of Applied Chemistry, Xinjiang University, Urumqi 830046, PR China.
authors: Guancheng
authors: G
authors: Xu
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China.
authors: Fuchun
authors: F
authors: Zhang
authors: Institute of Applied Chemistry, Xinjiang University, Urumqi 830046, PR China.
authors: Lang
authors: L
authors: Liu
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China; Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, TX 77030, USA.
authors: Jianhua
authors: J
authors: Yang
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, PR China. Electronic address: sr_sun2005@163.com.
authors: Surong
authors: S
authors: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002104:Cadmium / Q000737:chemistry / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D047069:Pyrazolones / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents; D047069:Pyrazolones; D017382:Reactive Oxygen Species; D002104:Cadmium; C038362:pyrazolone; D020169:Caspases
keywords: Apoptosis; Cadmium; Eca-109 cells; Mitochondrial pathway; Pyrazolone-based derivative