title: Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module.
abstract: The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221, with unit-cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P43212 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P41212, with unit-cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.
authors: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors: San
authors: S
authors: Hadzi
authors: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors: Abel
authors: A
authors: Garcia-Pino
authors: Department of Biology, University of Copenhagen, Copenhagen, Denmark.
authors: Kenn
authors: K
authors: Gerdes
authors: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ascerceva 5, 1000 Ljubljana, Slovenia.
authors: Jurij
authors: J
authors: Lah
authors: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors: Remy
authors: R
authors: Loris
mesh_terms: D000992:Antitoxins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D009875:Operator Regions, Genetic; D014734:Vibrio cholerae / Q000737:chemistry*
chemical_list: D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004247:DNA
keywords: macromolecular complex; persistence; protein–DNA complex; toxin–antitoxin module