title: Polyacrylic acid coated and non-coated iron oxide nanoparticles are not genotoxic to human T lymphocytes.
abstract: The use of iron oxide nanoparticles (ION) for diagnostic and therapeutic purposes requires a clear favorable risk-benefit ratio. This work was performed with the aim of studying the ability of polyacrylic acid (PAA)-coated and non-coated ION to induce genotoxicity in human T lymphocytes. For that purpose, their influence on cell cycle progression and on the induction of chromosome aberrations was evaluated. Blood samples collected from healthy human donors were exposed to PAA-coated and non-coated ION, at different concentrations, for 48h. The obtained results showed that, for all culture conditions, the tested ION are not genotoxic and do not influence the cell cycle arrest. Their possible cumulative effect with the iron-dependent genotoxic agent BLM was also evaluated. Blood samples collected from healthy human donors were exposed to ION, at different concentrations, for 48h, in the presence of a pre-determined toxic concentration of BLM. The obtained results showed that, for all culture conditions, the tested ION do not potentiate the clastogenic effects of BLM.
authors: UCIBIO/REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors: Diana
authors: D
authors: Couto
authors: Laboratory of Cytogenetics, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors: Rosa
authors: R
authors: Sousa
authors: Laboratory of Cytogenetics, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors: Lara
authors: L
authors: Andrade
authors: Service of Clinical Hematology, Santo António Hospital, Porto, Portugal.
authors: Magdalena
authors: M
authors: Leander
authors: Laboratory of Nanotechnology and Magnetism, Institute of Technological Research, IIT, University of Santiago de Compostela (USC), Spain.
authors: M Arturo
authors: MA
authors: Lopez-Quintela
authors: International Iberian Nanotechnology Laboratory, Braga, Portugal.
authors: José
authors: J
authors: Rivas
authors: International Iberian Nanotechnology Laboratory, Braga, Portugal.
authors: Paulo
authors: P
authors: Freitas
authors: Service of Clinical Hematology, Santo António Hospital, Porto, Portugal.
authors: Margarida
authors: M
authors: Lima
authors: Service of Clinical Hematology, Santo António Hospital, Porto, Portugal.
authors: Graça
authors: G
authors: Porto
authors: Laboratory of Cytogenetics, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors: Beatriz
authors: B
authors: Porto
authors: UCIBIO/REQUIMTE Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. Electronic address: felixdc@ff.up.pt.
authors: Félix
authors: F
authors: Carvalho
authors: UCIBIO/REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. Electronic address: egracas@ff.up.pt.
authors: Eduarda
authors: E
authors: Fernandes
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000633:toxicity*; D005296:Ferrous Compounds / Q000633:toxicity*; D006801:Humans; D053768:Metal Nanoparticles; D009152:Mutagenicity Tests; D018570:Risk Assessment; D013601:T-Lymphocytes / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D005290:Ferric Compounds; D005296:Ferrous Compounds; D001761:Bleomycin; C000499:ferric oxide; C006903:carbopol 940; C034236:ferrous oxide
keywords: Bleomycin; Cell cycle; Genotoxicity; Human T lymphocytes; Iron oxide nanoparticles