title: Improved production of isomaltulose by a newly isolated mutant of Serratia sp. cells immobilized in calcium alginate.
abstract: Isomaltulose, also known as palatinose, is produced by sucrose isomerase and has been highlighted as a sugar substitute due to a number of advantageous properties. For the massive production of isomaltulose, high resistance to sucrose and stability of sucrose isomerase as well as sucrose conversion yields would be critical factors. We describe a series of screening procedures to isolate the mutant strain of Serratia sp. possessing enhanced isomaltulose production with improved stability. The new Serratia sp. isolated from a series of screening procedures allowed us to produce isomaltulose from 60% sucrose solution, with over 90% conversion yield. Moreover, when this strain was immobilized in calcium alginate beads and placed in a medium containing 60% sucrose, it showed over 70% sucrose conversion yields for 30 cycles of repeated-batch reactions. Thus, improved conversion activity and stability of the newly isolated Serratia sp. strain in the present study would be highly valuable for industries related to isomaltulose production.
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Korea.
authors: Yonghwan
authors: Y
authors: Kim
authors: Bong-Seong
authors: BS
authors: Koo
authors: Hyeon-Cheol
authors: HC
authors: Lee
authors: Youngdae
authors: Y
authors: Yoon
mesh_terms: D000464:Alginates / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000096:biosynthesis; D016296:Mutagenesis; D009154:Mutation; D012705:Serratia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D006603:Hexuronic Acids; D013395:Sucrose; D007534:Isomaltose; D020723:Glucuronic Acid; D005964:Glucosyltransferases; C116853:sucrose isomerase; C008189:isomaltulose
keywords: Serratia sp.; biotransformation; calcium alginate immobilization; immobilisation au calcium alginate; isomaltulose