title: A miR-29c binding site genetic variant in the 3'-untranslated region of LAMTOR3 gene is associated with gastric cancer risk.
abstract: Single nucleotide polymorphisms (SNPs) in the 3'-untranslated regions (UTRs) targeted by putative mircoRNAs (miRNAs) could influence the susceptibility of cancer. Recently, miR-29c has been reported to be down-regulated in gastric cancer (GC) and serve as a tumor suppressor that regulated tumor progression. The present study was aimed at investigating whether the miR-29c binding site SNPs within the 3'-UTRs of target genes affected the gastric cancer risk. Using bioinformatics tools, we chose three SNPs (IGHMBP2 rs3750980, LAMTOR3 rs11944405 and WWOX rs2288035) located in miR-29c binding sites. We genotyped these three SNPs to assess their associations with GC risk in a case-control study comprising 753 GC cases and 950 controls. Among these three SNPs, we found a significantly decreased risk of GC associated with the LAMTOR3 rs11944405 T>C polymorphism [TC vs. TT, adjusted odds ratio (OR)=0.79, 95% confidence interval (CI)=0.63-0.99; TC/CC vs. TT, adjusted OR=0.81, 95% CI=0.65-1.00]. The significant association was also presented in the subgroup analysis by age (≤65), sex (female), depth of invasion (T3/T4), lymph node metastasis (N1-3), distant metastasis (M0) and TNM stage (III/IV). However, no significant association was detected for IGHMBP2 rs3750980 and WWOX rs2288035. Our results suggested that the LAMTOR3 rs11944405 polymorphism may be a potential biomarker for genetic susceptibility to GC.
authors: Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Peng
authors: P
authors: Song
authors: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Weizhi
authors: W
authors: Wang
authors: Department of General Surgery, Huai-An First People's Hospital Affiliated to Nanjing Medical University, Huai-an, China.
authors: Guoquan
authors: G
authors: Tao
authors: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Haiyan
authors: H
authors: Chu
authors: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Meilin
authors: M
authors: Wang
authors: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Dongmei
authors: D
authors: Wu
authors: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Na
authors: N
authors: Tong
authors: Department of General Surgery, Yixing Cancer Hospital, Yixing, China.
authors: Weida
authors: W
authors: Gong
authors: Department of Molecular Cell Biology & Toxicology, School of Public Health, Cancer Center, Nanjing Medical University, Nanjing, China.
authors: Jianwei
authors: J
authors: Zhou
authors: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
authors: Zhengdong
authors: Z
authors: Zhang
authors: Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: wangbl@njmu.edu.cn.
authors: Baolin
authors: B
authors: Wang
authors: Core Laboratory of Nantong Tumor Hospital, Nantong, China. Electronic address: 00zlingling@163.com.
authors: Haixia
authors: H
authors: Zhu
authors: Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: njzhqh@sina.com.
authors: Qinghong
authors: Q
authors: Zhao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000368:Aged; D003710:Demography; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; C485425:LAMTOR3 protein, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs
keywords: Gastric cancer; Genetic susceptibility; LAMTOR3; Single nucleotide polymorphism; miR-29c