title: Isolation and full characterisation of a potentially allergenic lipid transfer protein (LTP) in almond.
abstract: Non-specific lipid transfer proteins (nsLTP) were shown to be among the most significant allergens, in particular in several fruits belonging to the Rosaceae family. The molecular features of LTPs, such as the presence of eight cysteine residues forming four disulfide bridges, confer a compact structure, decreasing the probability of degradation due to cooking or digestion, thereby increasing the chance of systemic absorption and severe allergic reactions. Few studies on LTP-induced allergies regarding almond (Prunus dulcis L) are available in the literature. In the present work, we describe for the first time the extraction and purification of an almond LTP, achieving its full characterisation by using liquid chromatography and exact mass spectrometry; the full sequence was identified by means of LC-ESI-Orbitrap-MS applying a bottom-up approach. The characterised protein consists of 92 amino acids and has a calculated exact MW of 9579.0. The presence of four disulfide bridges was confirmed after reduction, as shown by a mass increment of 8 Da. Finally, its potential allergenicity was confirmed via an in silico approach. The results presented here demonstrate the enormous potential of advanced MS techniques for obtaining high-quality structural and functional data of allergenic proteins in a short time.
authors: a Department of Food Science , University of Parma , Parma , Italy.
authors: Sofie
authors: S
authors: Buhler
authors: Tullia
authors: T
authors: Tedeschi
authors: Andrea
authors: A
authors: Faccini
authors: Cristiano
authors: C
authors: Garino
authors: Marco
authors: M
authors: Arlorio
authors: Arnaldo
authors: A
authors: Dossena
authors: Stefano
authors: S
authors: Sforza
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis; D009754:Nuts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000068181:Prunus dulcis / Q000737:chemistry*
chemical_list: D000485:Allergens; D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein
keywords: LTP; LTQ-Orbitrap; almonds; food allergy; proteomics