title: Methods for monitoring lysosomal morphology.
abstract: Lysosomes are abundant organelles best known for their crucial role in macromolecule turnover. Lysosome dysfunction features in several diseases exemplified by the lysosomal storage disorders and is often associated with marked changes in lysosome structure. Lysosomal morphology may therefore serve as a sensitive readout of endocytic well-being. Here we describe methods for monitoring lysosome morphology in fixed and live cells using fluorescent probes and electron microscopy.
authors: Department of Cell and Developmental Biology, University College London, London, UK.
authors: Bethan S
authors: BS
authors: Kilpatrick
authors: Department of Cell Biology, Institute of Ophthalmology, University College London, London, UK.
authors: Emily R
authors: ER
authors: Eden
authors: Department of Cell and Developmental Biology, University College London, London, UK.
authors: Leanne N
authors: LN
authors: Hockey
authors: Department of Cell Biology, Institute of Ophthalmology, University College London, London, UK.
authors: Clare E
authors: CE
authors: Futter
authors: Department of Cell and Developmental Biology, University College London, London, UK.
authors: Sandip
authors: S
authors: Patel
mesh_terms: D002478:Cells, Cultured; D020285:Cryoelectron Microscopy; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008247:Lysosomes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053140:Organelle Shape; D059010:Single-Cell Analysis; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D005456:Fluorescent Dyes
keywords: Electron microscopy; Endocytosis; Fluorescence microscopy; Immunofluorescence; LAMP1; Lysosome; Membrane traffic; NAADP; Two-pore channels