title: Use of hyperdry amniotic membrane in operations for cleft palate: a study in rats.
abstract: The growth of maxillary bone and the development of dentition are often impaired in patients who have had pushback operations for repair of a cleft palate. There has been considerable discussion about the most suitable technique or material used in such repairs to resolve the problem. Hyperdry amniotic membrane, a new preservable material derived from human amnion, has recently been introduced in several procedures. We have evaluated its use during pushback surgery in animal studies to try to correct the inhibition of growth and development of the maxilla. Mucosal defects were created in 3-week-old rats, and then covered with hyperdry amniotic membrane or not. Healing was assessed by histological and morphological examination at 1 week and 7 weeks postoperatively. In the group treated with hyperdry amniotic membrane, submucosal tissue was reconstructed successfully during the early postoperative period. Lateral palatal growth was not inhibited as much, and medial inclination of the teeth was less, after a period of growth using this material. The results suggest that hyperdry amniotic membrane is a suitable new dressing material for use in the treatment of cleft palate.
authors: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan; Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan. Electronic address: htsuno-tym@umin.ac.jp.
authors: Hiroaki
authors: H
authors: Tsuno
authors: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan.
authors: Makoto
authors: M
authors: Noguchi
authors: Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan.
authors: Motonori
authors: M
authors: Okabe
authors: Department of Oral and Maxillofacial Surgery, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan.
authors: Kei
authors: K
authors: Tomihara
authors: Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan.
authors: Toshiko
authors: T
authors: Yoshida
authors: Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Japan.
authors: Toshio
authors: T
authors: Nikaido
mesh_terms: D000650:Amnion; D000818:Animals; D001684:Biological Dressings; D002972:Cleft Palate / Q000473:pathology / Q000601:surgery*; D003487:Cyanoacrylates / Q000627:therapeutic use; D003724:Dental Arch / Q000254:growth & development / Q000473:pathology; D004195:Disease Models, Animal; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008297:Male; D008437:Maxilla / Q000254:growth & development / Q000473:pathology; D008963:Molar / Q000473:pathology; D009061:Mouth Mucosa / Q000473:pathology / Q000601:surgery; D010159:Palate / Q000254:growth & development / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures / Q000379:methods*; D013997:Time Factors; D014014:Tissue Adhesives / Q000627:therapeutic use; D014021:Tissue Preservation / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate
keywords: Amnion; Cleft palate; Cleft repair; Constriction of dental arch; Growth of maxilla; Scarring of wound