title: High expression of kinesin light chain-2, a novel target of miR-125b, is associated with poor clinical outcome of elderly non-small-cell lung cancer patients.
abstract: BACKGROUND
  MiR-125b has critical role in non-small-cell lung cancer (NSCLC) cell migration, and its target genes have not been elucidated. Kinesin-1 light chain (KLC)-2 was predicted as one of miR-125b's targets by bioinformatics analysis. This study is to identify the function of KLC2 and its interaction with miR-125b in NSCLC.
  
  
  METHODS
  Kinesin-1 light chain-2 protein expression and its clinical relevance were analysed in 140 matched NSCLC and adjacent non-neoplastic lung tissues. Both KLC2 gain- and loss-of-function analyses were performed in NSCLC cell lines by transient transfection. The direct interaction between KLC2 and miR-125b was confirmed by a luciferase reporter assay and a transient co-transfection assay as well as an analysis of eight matched clinical samples.
  
  
  RESULTS
  KLC2 protein was upregulated in NSCLC cell lines and tissues, and was an independent predictor of poor prognosis for elderly NSCLC patients. Kinesin-1 light chain-2 remarkably enhanced the invasive and migratory ability of NSCLC cells. MiR-125b inhibited KLC2 3'-untranslated region luciferase activity and protein expression, and inversely correlated with KLC2 expression in clinical samples. Kinesin-1 light chain-2 almost completely reversed miR-125b-induced inhibition on migration and invasion.
  
  
  CONCLUSIONS
  Kinesin-1 light chain-2 protein overexpression predicts poor survival in elderly NSCLC patients. Kinesin-1 light chain-2 acts as a proto-oncogene and a functional target of miR-125b in NSCLC cells.
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: M
authors: M
authors: Wang
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: X
authors: X
authors: Zhu
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: Z
authors: Z
authors: Sha
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: N
authors: N
authors: Li
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: D
authors: D
authors: Li
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Republic of China.
authors: L
authors: L
authors: Chen
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016547:Kinesins; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D000090063:Proto-Oncogene Mas
chemical_list: D020413:3' Untranslated Regions; C000715470:MAS1 protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D000090063:Proto-Oncogene Mas; D016547:Kinesins