title: Effect of net surface charge on physical properties of the cellulose nanoparticles and their efficacy for oral protein delivery.
abstract: Both net positively and negatively charged cellulose-based nanoparticles were prepared from oppositely charged carboxymethylcellulose (CMC) and quaternized cellulose (QC). Effect of surface charge on efficacy of cellulose nanoparticles for delivering both positively and negatively charged proteins was investigated. Lysozyme (LYS) and bovine serum albumin (BSA), which possess positive and negative charge at physiological pH respectively, were used as models. The results revealed that high encapsulation efficiency (67.7% and 85.1%) could be achieved when negatively charged protein was encapsulated in positively charged nanoparticles, or positively charged protein was encapsulated in negatively charged nanoparticles. Proteins encapsulated in optimal cellulose nanoparticles could be sustainably released and no obvious protein denaturation was detected. Both net positively and negatively charged nanoparticles exhibited low cytotoxicity due to cellulose's good biocompatibility. Not only net positively charged nanoparticles demonstrated high cellular uptake efficiency, but also net negatively charged nanoparticles showed somewhat efficient cellular uptake.
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, T6G 2P5, Canada.
authors: Yongbo
authors: Y
authors: Song
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, T6G 2P5, Canada. Electronic address: lingyun.chen@ualberta.ca.
authors: Lingyun
authors: L
authors: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D065546:Drug Liberation; D006801:Humans; D008958:Models, Molecular; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D055585:Physical Phenomena; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D009113:Muramidase; D002266:Carboxymethylcellulose Sodium
keywords: Cellular uptake; Cellulose nanoparticles; Oral delivery; Protein carrier; Surface charge