title: Lentivirus-mediated inhibition of tumour necrosis factor-α improves motor function associated with PRDX6 in spinal cord contusion rats.
abstract: The recovery of motor function in rats is inhibited following contusion spinal cord injury (cSCI). However, the mechanism of tumour necrosis factor α (TNF-α) in motor function after cSCI associated with peroxiredoxin 6 (PRDX6) remains unknown. We randomly divided rats into four groups: sham, cSCI, vector and lentivirus mediating TNF-α RNA interference (TNF-α-RNAi-LV) group. The Basso, Beattie, Bresnahan (BBB) scale was used to evaluate motor function. Real-time quantitative PCR (qRT-PCR) and western blotting were used to detect the expression of TNF-α and PRDX6, which were located in neurons using immunohistochemistry (IHC) and immunofluorescence. Subsequently, lentiviral-mediated TNF-α was used to determine the role of TNF-αand the relationship of PRDX6 and TNF-α in cSCI. After cSCI, the motor capability of hind limbs disappeared and was followed by recovery of function. IHC analysis indicated that TNF-α and PRDX6 were primarily located in spinal cord neurons. TNF-α interference significantly improved neural behaviour and increased expression of PRDX6. Our study suggests that inhibition of TNF-α can promote the recovery of motor function. The underlying mechanism of TNF-α-promoted motor function may be connected with the up-regulation of PRDX6. This provides a new strategy or target for the clinical treatment of SCI in future.
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Xiao
authors: X
authors: Zhang
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Lan-lan
authors: LL
authors: Shi
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Xia
authors: X
authors: Gao
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Di
authors: D
authors: Jiang
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Zhan-qiong
authors: ZQ
authors: Zhong
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Xi
authors: X
authors: Zeng
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Ying
authors: Y
authors: Rao
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Xi
authors: X
authors: Hu
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Tian-zhi
authors: TZ
authors: Li
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Xiu-juan
authors: XJ
authors: Li
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Lei
authors: L
authors: Li
authors: Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
authors: Jian-min
authors: JM
authors: Chen
authors: Institute of Neurological Diseases, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Qingjie
authors: Q
authors: Xia
authors: 1] Institute of Neuronscience, Kunming Medical University, Kunming 650031, China [2] Institute of Neurological Diseases, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Ting-hua
authors: TH
authors: Wang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D009043:Motor Activity / Q000235:genetics*; D054465:Peroxiredoxin VI / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D051381:Rats; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D014161:Transduction, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D054465:Peroxiredoxin VI