title: Probing in vitro ribose induced DNA-glycation using Raman microspectroscopy.
abstract: To identify and characterize glycation, induced modifications of DNA are crucial toward understanding their functional significance due to their significant role in the long term control of aging and age-related diseases. In this study, we present the ability of Raman microspectroscopy as a novel analytical technique for a rapid and reliable identification of glycated DNA in a reagent-free manner. We have demonstrated that this technique has potential to provide very small conformational modifications. The combination of principal component analysis (PCA) and two-dimensional (2D) correlation spectroscopy has assisted us to explore in vitro DNA-glycation and provide more insights into the dynamics of the DNA-glycation process in an easier fashion. PCA analysis of Raman spectra shows a clear discrimination between native and glycated DNA samples. On the other hand, 2D correlation Raman analysis provides sequential order of the mechanism of the DNA-glycation process, and most likely, it occurs in the following sequence: Structural modifications of individual nucleobases (G > A > C) → DNA backbone modifications → partial transition of DNA conformations (A to B form). Our observations clearly suggest that the structure of DNA is altered, i.e., a partial transition of DNA backbone conformation from A to B form when glycated, but does not induce any final transition in DNA double helix conformation, and eventually, DNA presents in an intermediate A-B form, more toward the B form.
authors: MéDIAN Biophotonique et Technologies pour la Santé, Université de Reims Champagne-Ardenne, CNRS UMR 7369 MEDyC, UFR de Pharmacie, SFR CAP Santé , 51096 Reims Cedex, France.
authors: Goutam Kumar
authors: GK
authors: Chandra
authors: Christophe
authors: C
authors: Eklouh-Molinier
authors: Michael
authors: M
authors: Fere
authors: Jean-François
authors: JF
authors: Angiboust
authors: Cyril
authors: C
authors: Gobinet
authors: Laurence
authors: L
authors: Van-Gulick
authors: Pierre
authors: P
authors: Jeannesson
authors: Olivier
authors: O
authors: Piot
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D006031:Glycosylation; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012266:Ribose / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; D012266:Ribose; D004247:DNA; C427203:calf thymus DNA