title: Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production.
abstract: (3R)-Acetoin and (2R,3R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3R)-acetoin and (2R,3R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2S,3S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2S,3S)-2,3-butanediol and result in a large quantity of (3R)-acetoin accumulation. Furthermore, a (2R,3R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2R,3R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol and lead to (2R,3R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2R,3R)-2,3-butanediol dehydrogenase expression strain could produce 89.81 g/l (2R,3R)-2,3-butanediol with a productivity of 1.91 g/l/h at 48 h. These results provided potential applications for (3R)-acetoin and (2R,3R)-2,3-butanediol production.
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors: Fangmin
authors: F
authors: Bai
authors: Lu
authors: L
authors: Dai
authors: Jiying
authors: J
authors: Fan
authors: Ngoctu
authors: N
authors: Truong
authors: Ben
authors: B
authors: Rao
authors: Liaoyuan
authors: L
authors: Zhang
authors: Yaling
authors: Y
authors: Shen
mesh_terms: D000093:Acetoin / Q000737:chemistry / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering; D012706:Serratia marcescens / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000093:Acetoin; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase