title: RAPTR-SV: a hybrid method for the detection of structural variants.
abstract: BACKGROUND
  Identification of structural variants (SVs) in sequence data results in a large number of false positive calls using existing software, which overburdens subsequent validation.
  
  
  RESULTS
  Simulations using RAPTR-SV and other, similar algorithms for SV detection revealed that RAPTR-SV had superior sensitivity and precision, as it recovered 66.4% of simulated tandem duplications with a precision of 99.2%. When compared with calls made by Delly and LUMPY on available datasets from the 1000 genomes project, RAPTR-SV showed superior sensitivity for tandem duplications, as it identified 2-fold more duplications than Delly, while making ∼85% fewer duplication predictions.
  
  
  METHODS
  RAPTR-SV is written in Java and uses new features in the collections framework in the latest release of the Java version 8 language specifications. A compiled version of the software, instructions for usage and test results files are available on the GitHub repository page: https://github.com/njdbickhart/RAPTR-SV.
  
  
  BACKGROUND
  derek.bickhart@ars.usda.gov.
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Derek M
authors: DM
authors: Bickhart
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Jana L
authors: JL
authors: Hutchison
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Lingyang
authors: L
authors: Xu
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Robert D
authors: RD
authors: Schnabel
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Jeremy F
authors: JF
authors: Taylor
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: James M
authors: JM
authors: Reecy
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Steven
authors: S
authors: Schroeder
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Curt P
authors: CP
authors: Van Tassell
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: Tad S
authors: TS
authors: Sonstegard
authors: Animal Genomics and Improvement Laboratory, ARS, USDA, Beltsville, MD 20705, USA, Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20705, USA, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA and Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors: George E
authors: GE
authors: Liu
mesh_terms: D000465:Algorithms; D002874:Chromosome Mapping; D015894:Genome, Human; D056914:Genomic Structural Variation; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion; D012984:Software