title: Biological safety evaluation of the modified urinary catheter.
abstract: The purpose of this study was to evaluate in vitro safety of the novel tosufloxacin (TOS)-treated catheters with the prolonged antimicrobial activity. The test samples of silicone latex catheter were prepared by the immobilization of TOS on chitosan (CHIT)-coated catheter by means of covalent bonds and non-covalent interactions. Each step of the modification process of catheter surface was observed using ATR-Fourier transform infrared spectroscopy. In vitro cytotoxicity of the modified and unmodified catheters was assessed by direct and indirect tests in accordance with ISO standards using green monkey kidney (GMK) cell line. The MTT, lactate dehydrogenase activity (LDH), WST-8, Sulforhodamine B (SRB) test results and microscopic observation clearly indicated that unmodified silicone latex catheters decrease cell metabolic activity, act as a cytotoxic agent causing cell lysis and induce cell death through necrotic or apoptotic process. We suggest that chitosan coat with TOS immobilized limits leaching of harmful agents from silicone latex material, which significantly enhances survivability of GMK cells and therefore is quite a good protection against the cytotoxic effect of this material.
authors: Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland. Electronic address: dorota.kowalczuk@umlub.pl.
authors: Dorota
authors: D
authors: Kowalczuk
authors: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland.
authors: Agata
authors: A
authors: Przekora
authors: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland.
authors: Grazyna
authors: G
authors: Ginalska
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000009:adverse effects / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D002522:Chlorocebus aethiops; D024841:Fluoroquinolones / Q000009:adverse effects* / Q000737:chemistry*; D007840:Latex / Q000737:chemistry; D009287:Naphthyridines / Q000009:adverse effects* / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry; D062885:Urinary Catheters / Q000009:adverse effects*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007840:Latex; D009287:Naphthyridines; D012828:Silicones; D048271:Chitosan; C055185:tosufloxacin
keywords: Cytotoxicity; Surface characterization; Tosufloxacin; Urinary catheterization