title: The Sinorhizobium fredii HH103 Genome: A Comparative Analysis With S. fredii Strains Differing in Their Symbiotic Behavior With Soybean.
abstract: Sinorhizobium fredii HH103 is a fast-growing rhizobial strain infecting a broad range of legumes including both American and Asiatic soybeans. In this work, we present the sequencing and annotation of the HH103 genome (7.25 Mb), consisting of one chromosome and six plasmids and representing the structurally most complex sinorhizobial genome sequenced so far. Comparative genomic analyses of S. fredii HH103 with strains USDA257 and NGR234 showed that the core genome of these three strains contains 4,212 genes (61.7% of the HH103 genes). Synteny plot analysis revealed that the much larger chromosome of USDA257 (6.48 Mb) is colinear to the HH103 (4.3 Mb) and NGR324 chromosomes (3.9 Mb). An additional region of the USDA257 chromosome of about 2 Mb displays similarity to plasmid pSfHH103e. Remarkable differences exist between HH103 and NGR234 concerning nod genes, flavonoid effect on surface polysaccharide production, and quorum-sensing systems. Furthermore a number of protein secretion systems have been found. Two genes coding for putative type III-secreted effectors not previously described in S. fredii, nopI and gunA, have been located on the HH103 genome. These differences could be important to understand the different symbiotic behavior of S. fredii strains HH103, USDA257, and NGR234 with soybean.
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: José-María
authors: JM
authors: Vinardell
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: Sebastián
authors: S
authors: Acosta-Jurado
authors: 2 Technische Universität Dresden, Institut für Genetik, Helmholtzstrasse 10, 01062 Dresden, Germany;
authors: Susanne
authors: S
authors: Zehner
authors: 2 Technische Universität Dresden, Institut für Genetik, Helmholtzstrasse 10, 01062 Dresden, Germany;
authors: Michael
authors: M
authors: Göttfert
authors: 3 LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Faculty of Biology, Philipps-Universität Marburg, Hans-Meerwein-Str. 6, 35043 Marburg, Germany;
authors: Anke
authors: A
authors: Becker
authors: 4 Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049-Madrid, Spain;
authors: Irene
authors: I
authors: Baena
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Jochem
authors: J
authors: Blom
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: Juan Carlos
authors: JC
authors: Crespo-Rivas
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Alexander
authors: A
authors: Goesmann
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Sebastian
authors: S
authors: Jaenicke
authors: 3 LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Faculty of Biology, Philipps-Universität Marburg, Hans-Meerwein-Str. 6, 35043 Marburg, Germany;
authors: Elizaveta
authors: E
authors: Krol
authors: 3 LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Faculty of Biology, Philipps-Universität Marburg, Hans-Meerwein-Str. 6, 35043 Marburg, Germany;
authors: Matthew
authors: M
authors: McIntosh
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: Isabel
authors: I
authors: Margaret
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: Francisco
authors: F
authors: Pérez-Montaño
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Susanne
authors: S
authors: Schneiker-Bekel
authors: 3 LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Faculty of Biology, Philipps-Universität Marburg, Hans-Meerwein-Str. 6, 35043 Marburg, Germany;
authors: Javier
authors: J
authors: Serranía
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Rafael
authors: R
authors: Szczepanowski
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: Ana-María
authors: AM
authors: Buendía
authors: 4 Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049-Madrid, Spain;
authors: Javier
authors: J
authors: Lloret
authors: 4 Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049-Madrid, Spain;
authors: Ildefonso
authors: I
authors: Bonilla
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Alfred
authors: A
authors: Pühler
authors: 1 Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes 6, C.P. 41012 Sevilla, Spain;
authors: José-Enrique
authors: JE
authors: Ruiz-Sainz
authors: 5 Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Universitaetsstr. 27, 33615 Bielefeld, Germany.
authors: Stefan
authors: S
authors: Weidner
mesh_terms: D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000235:genetics; D053038:Quorum Sensing; D042181:Sinorhizobium fredii / Q000235:genetics* / Q000502:physiology; D013025:Glycine max / Q000382:microbiology*; D013559:Symbiosis / Q000235:genetics
chemical_list: D011135:Polysaccharides, Bacterial