title: Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter.
abstract: Diverse exopolysaccharide (EPS)-producing isolates were isolated from an Indian acidic fermented food (Idli) based on the colony morphology. One of the EPS-producing microflora (Leuconostoc lactis KC117496) was selected for further characterization using FT-IR, HPTLC, AFM, SEM, TGA and XRD analysis. FT-IR spectroscopy revealed the α-d-glucose nature of the EPS. HPTLC analysis confirmed the presence of only glucose monomers, indicating the glucan nature of EPS. NMR spectra revealed the presence of 95% α-(1→6) and 5% branching α-(1→3) linkages. The SEM and AFM showed smooth surfaces and compact structure. TGA results showed higher degradation temperature of 272.01°C. XRD analysis proved the 33.4% crystalline nature of the EPS. Water solubility index and water-holding capacity of EPS are 14.2±0.208% and 117±7.5%. All the above characteristics of the EPS produced by L. lactis showed that the EPS is of a good-quality polysaccharide with potential applications in the food industry.
authors: Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.
authors: Chinnashanmugam
authors: C
authors: Saravanan
authors: Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India. Electronic address: pkshalady@yahoo.co.uk.
authors: Prathap Kumar H
authors: PK
authors: Shetty
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002855:Chromatography, Thin Layer; D015169:Colony Count, Microbial; D005433:Flour / Q000382:microbiology*; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D007934:Leuconostoc / Q000378:metabolism* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification* / Q000648:ultrastructure; D066244:Proton Magnetic Resonance Spectroscopy; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D011135:Polysaccharides, Bacterial; D014867:Water; D005947:Glucose
keywords: Exopolysaccharide; Idli batter; Leuconostoc lactis