title: Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells.
abstract: Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells.
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Yingying
authors: Y
authors: Cong
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Xiaoxue
authors: X
authors: Li
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Yunyun
authors: Y
authors: Bai
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience & Technology of China, Beijing 100090, China.
authors: Xiaonan
authors: X
authors: Lv
authors: Institute for Virology, University of Veterinary Medicine, Hannover D-30559, Germany.
authors: Georg
authors: G
authors: Herrler
authors: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors: Luis
authors: L
authors: Enjuanes
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Xingdong
authors: X
authors: Zhou
authors: Faculty of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
authors: Bo
authors: B
authors: Qu
authors: Institute for Virology, University of Veterinary Medicine, Hannover D-30559, Germany.
authors: Fandan
authors: F
authors: Meng
authors: College Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China.
authors: Chengcheng
authors: C
authors: Cong
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Xiaofeng
authors: X
authors: Ren
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Guangxing
authors: G
authors: Li
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D054884:Host-Pathogen Interactions; D007421:Intestine, Small / Q000821:virology; D046529:Microscopy, Electron, Transmission; D053485:Porcine epidemic diarrhea virus / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D011991:Receptors, Virus / Q000378:metabolism*; D013552:Swine; D053586:Virus Internalization; D057074:Virus Release
chemical_list: D011991:Receptors, Virus; D018826:CD13 Antigens
keywords: Epithelial; PEDV; Receptor; pAPN