title: Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group.
abstract: The reaction between (4-nitrobenzoylmethylene)triphenylphosphorane Pd(II) complex [Pd{κ(2)(C,C)-C₆ H₄PPh₂C(H)CO(C₆ H₄NO₂-4)}(μ-Cl)]₂ and excess of NaN₃ resulted in the μ-N₃ bridged Pd(II) complex [Pd{κ(2)(C,C)-C₆H₄PPh₂C(H)CO(C₆ H₄NO₂-4)}(μ-N₃)]₂ (1), which underwent bridge cleavage reactions with monodentate ligands to afford the monomeric, neutral complexes [Pd{κ(2)(C,C)-C₆ H₄PPh₂C(H)CO(C₆ H₄NO₂-4)}N₃(L)] (L=Me₃Py (1a), PPh₃ (1b)). The complexes were identified and characterized by elemental analyses, infrared (IR), ((1))H, ((13))C{((1))H} and ((31))P{((1))H} NMR spectroscopy. The molecular structure of 1b was determined by single-crystal X-ray diffraction. The interactions of complexes with FS-DNA were investigated using UV absorption and fluorescence spectra. The results suggested that both complexes could interact with FS-DNA through the intercalation mode and follow the binding affinity order of 1a>1b. The reactivity toward protein BSA revealed that the quenching of BSA fluorescence by the two complexes are static quenching, and complex 1a exhibits a higher BSA-binding ability than the complex 1b.
authors: Department of Chemistry, Isfahan University of Technology, Isfahan 84156/83111, Iran. Electronic address: karami@cc.iut.ac.ir.
authors: Kazem
authors: K
authors: Karami
authors: Department of Chemistry, Isfahan University of Technology, Isfahan 84156/83111, Iran.
authors: Zahra
authors: Z
authors: Shirani-Sarmazeh
authors: Department of Chemistry, Isfahan University of Technology, Isfahan 84156/83111, Iran.
authors: Mahboubeh
authors: M
authors: Hosseini-Kharat
authors: Cardinal Stefan Wyszynski University in Warsaw - Faculty of Mathematical and Natural Sciences, Wojcickiego 1/3, 02-093 Warszawa, Poland.
authors: Janusz
authors: J
authors: Lipkowski
authors: Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.
authors: Maryam
authors: M
authors: Saeidifar
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D010165:Palladium / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D001386:Azides; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; D010758:Phosphorus; D010165:Palladium; D004247:DNA