title: A novel FRET 'off-on' fluorescent probe for the selective detection of Fe³⁺, Al³⁺ and Cr³⁺ ions: its ultrafast energy transfer kinetics and application in live cell imaging.
abstract: A rhodamine-naphthalimide dyad probe, 1, that selectively responds to the addition of trivalent metal ions (Fe(3+) or Al(3+) or Cr(3+)) via ultrafast Förster resonance energy transfer (FRET) from naphthalimide to rhodamine is designed and synthesized. 1 is highly selective to the trivalent metal ions and the presence of other monovalent or divalent metal ions do not affect its detection ability. The probe is highly sensitive and it can respond to the presence of trivalent metal ions even at sub-micromolar levels. 1 is stable over a broad range of pH, non-toxic under experimental conditions and suitable to the fluorescence bio-imaging of live cells exposed to trivalent metal ions. The trivalent metal ion induced ultrafast energy transfer kinetics of 1 is explored using time resolved fluorescence experiments.
authors: Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India. Electronic address: chereddynarendra@gmail.com.
authors: Narendra Reddy
authors: NR
authors: Chereddy
authors: Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India.
authors: Peethani
authors: P
authors: Nagaraju
authors: Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India.
authors: M V
authors: MV
authors: Niladri Raju
authors: Biomaterials Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors: Venkat Raghavan
authors: VR
authors: Krishnaswamy
authors: Biomaterials Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors: Purna Sai
authors: PS
authors: Korrapati
authors: Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India.
authors: Prakriti Ranjan
authors: PR
authors: Bangal
authors: Crop Protection Chemicals Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India; AcSIR, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500 007, India.
authors: Vaidya Jayathirtha
authors: VJ
authors: Rao
mesh_terms: D000535:Aluminum / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D058948:Cell Tracking / Q000379:methods; D002857:Chromium / Q000737:chemistry / Q000302:isolation & purification*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000737:chemistry / Q000302:isolation & purification; D007501:Iron / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D057054:Molecular Imaging; D053644:Naphthalimides / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D053644:Naphthalimides; D012235:Rhodamines; D002857:Chromium; D000535:Aluminum; D007501:Iron
keywords: Live cell imaging; Ratiometric sensors; Rhodamine–naphthalimide dyad; Time resolved fluorescence; Trivalent metal ion detection