title: Armet is an effector protein mediating aphid-plant interactions.
abstract: Aphid saliva is predicted to contain proteins that modulate plant defenses and facilitate feeding. Armet is a well-characterized bifunctional protein in mammalian systems. Here we report a new role of Armet, namely as an effector protein in the pea aphid, Acyrthosiphon pisum. Pea aphid Armet's physical and chemical properties and its intracellular role are comparable to those reported for mammalian Armets. Uniquely, we detected Armet in aphid watery saliva and in the phloem sap of fava beans fed on by aphids. Armet's transcript level is several times higher in the salivary gland when aphids feed on bean plants than when they feed on an artificial diet. Knockdown of the Armet transcript by RNA interference disturbs aphid feeding behavior on fava beans measured by the electrical penetration graph technique and leads to a shortened life span. Inoculation of pea aphid Armet protein into tobacco leaves induced a transcriptional response that included pathogen-responsive genes. The data suggest that Armet is an effector protein mediating aphid-plant interactions.
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Wei
authors: W
authors: Wang
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Huaien
authors: H
authors: Dai
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Yi
authors: Y
authors: Zhang
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Raman
authors: R
authors: Chandrasekar
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Lan
authors: L
authors: Luo
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Yasuaki
authors: Y
authors: Hiromasa
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Changzhong
authors: C
authors: Sheng
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Gongxin
authors: G
authors: Peng
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Shaoliang
authors: S
authors: Chen
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: John M
authors: JM
authors: Tomich
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: John
authors: J
authors: Reese
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Owain
authors: O
authors: Edwards
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
authors: Le
authors: L
authors: Kang
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia cuif@ioz.ac.cn reeck@ksu.edu.
authors: Gerald
authors: G
authors: Reeck
authors: *State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia cuif@ioz.ac.cn reeck@ksu.edu.
authors: Feng
authors: F
authors: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004435:Eating / Q000502:physiology; D059865:Endoplasmic Reticulum Stress; D019143:Evolution, Molecular; D054884:Host-Pathogen Interactions / Q000502:physiology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D031307:Vicia faba / Q000378:metabolism / Q000469:parasitology*
chemical_list: D007074:Immunoglobulin G; D019476:Insect Proteins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides
keywords: MANF; calcium binding; feeding behavior; saliva protein; salivary gland