title: [Analysis of cell morphology and immunophenotypic characteristics in 47 cases of multiple myeloma].
abstract: OBJECTIVE
  This study was to investigate the cell morphology and cell immune phenotypic characteristics in patients with multiple myeloma (MM).
  
  
  METHODS
  The flow cytometry with multiparametric direct immunofluorescence technique, and CD45/SSC and CD38(+)(+)/CD138(+) gating were used to measure cell markers CD138, CD38, CD56, CD117, CD3, CD13, CD33, CD19, CD7, CD20, CD22, CD34, CD28 in 47 MM patients. At the same time the morphology examination of bone marrow cells was performed.
  
  
  RESULTS
  The suspicious myeloma cell ratio in MM patients was 9.42%-74.25% detected by flow cytometry, moreover, the myeloma cell ratio detected by morphology examination was 11.0%-80.6%, there was a good correlation between the two detection methods (r(2) = 0.54, P < 0.001). The ratio of antigen positive expression was as follows: 74.46% for CD138, 100% for CD38, 57.44% for CD56, 40.42% for CD117, 6.38% for CD13, 19.15% for CD33, 8.51% for CD20, 27.66% for CD28, 2.12% for CD22, 4.25% for CD34, 0% for CD3, 0% for CD19, 0% for CD7.
  
  
  CONCLUSIONS
  CD45/SSC and CD38(+)/CD138(+) gating technique can accurately gate multiple myeloma cell sets which need analysis, the majority of myeloma cells expreses CD138, CD38, CD56 antigens. The immunophenotypic analysis combined with the cell morphology examination more contribute to the diagnosis and differential diagnosis of multiple myeloma.
authors: Department of Clinical Laboratorial Examination, Nongken Nada Hospital,Nada 571700,Hainan Province,China.
authors: Xian-Du
authors: XD
authors: Su
authors: Department of Hematology, Nanfang Hospital of Southern Medical University,Guangzhou 510515, Guangdong Province, China.
authors: Rong
authors: R
authors: Lin
authors: Department of Hematology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors: Xiao-Lan
authors: XL
authors: Xu
authors: Department of Clinical Laboratorial Examination, The First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi Province, China.
authors: Xu
authors: X
authors: Chen
authors: Department of Clinical Laboratorial Examination, Nongken Nada Hospital, Nada 571700, Hainan Province, China.
authors: Wen-Li
authors: WL
authors: Zhan
authors: Department of Clinical Laboratorial Examination, Nongken Nada Hospital, Nada 571700, Hainan Province, China.
authors: Jin-Pu
authors: JP
authors: Zheng
authors: Department of Clinical Laboratorial Examination, Nongken Nada Hospital,Nada 571700, Hainan Province, China. E-mail: fzhl1209@163.com.
authors: Chang-Ling
authors: CL
authors: Fan
mesh_terms: D015703:Antigens, CD; D001854:Bone Marrow Cells; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D009101:Multiple Myeloma
chemical_list: D015703:Antigens, CD