title: How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure.
abstract: Toxicity of pesticides towards microorganisms can have a major impact on ecosystem function. Nevertheless, some microorganisms are able to respond quickly to this stress by degrading these molecules. The edaphic Bacillus megaterium strain Mes11 can degrade the herbicide mesotrione. In order to gain insight into the cellular response involved, the intracellular proteome of Mes11 exposed to mesotrione was analyzed using the two-dimensional differential in-gel electrophoresis (2D-DIGE) approach coupled with mass spectrometry. The results showed an average of 1820 protein spots being detected. The gel profile analyses revealed 32 protein spots whose abundance is modified after treatment with mesotrione. Twenty spots could be identified, leading to 17 non redundant proteins, mainly involved in stress, metabolic and storage mechanisms. These findings clarify the pathways used by B. megaterium strain Mes11 to resist and adapt to the presence of mesotrione.
authors: Clermont Université, Université Blaise Pascal, LMGE, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, Laboratoire Microorganismes: Génome et Environnement, F-63177 Aubière, France.
authors: Corinne
authors: C
authors: Bardot
authors: Clermont Université, Université Blaise Pascal, ICCF, F-63000 Clermont Ferrand, France; CNRS, UMR 6296, Institut de Chimie de Clermont-Ferrand, BP 80026, F-63171 Aubière Cedex, France.
authors: Pascale
authors: P
authors: Besse-Hoggan
authors: Clermont Université, Université Blaise Pascal, LMGE, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, Laboratoire Microorganismes: Génome et Environnement, F-63177 Aubière, France.
authors: Louis
authors: L
authors: Carles
authors: Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, Paris, France; Plate-forme Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors: Morgane
authors: M
authors: Le Gall
authors: Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, Paris, France; Plate-forme Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors: Guilhem
authors: G
authors: Clary
authors: Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, Paris, France; Plate-forme Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors: Philippe
authors: P
authors: Chafey
authors: Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, Paris, France; Plate-forme Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors: Christian
authors: C
authors: Federici
authors: Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, Paris, France; Plate-forme Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors: Cédric
authors: C
authors: Broussard
authors: Clermont Université, Université Blaise Pascal, LMGE, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, Laboratoire Microorganismes: Génome et Environnement, F-63177 Aubière, France. Electronic address: isabelle.batisson@univ-bpclermont.fr.
authors: Isabelle
authors: I
authors: Batisson
mesh_terms: D000222:Adaptation, Physiological; D001410:Bacillus megaterium / Q000378:metabolism / Q000502:physiology*; D003512:Cyclohexanones / Q000378:metabolism* / Q000633:toxicity; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D006540:Herbicides / Q000378:metabolism* / Q000633:toxicity; D020543:Proteome / Q000378:metabolism
chemical_list: D003512:Cyclohexanones; D006540:Herbicides; D020543:Proteome; C432907:mesotrione
keywords: 2D-DIGE; Differential proteomic; Pesticides; Physiological responses; Soil bacteria