title: Correlation analysis of peripheral DPYD gene polymorphism with 5-fluorouracil susceptibility and side effects in colon cancer patients.
abstract: OBJECTIVE
  To investigate the correlation of peripheral DPYD gene polymorphism with 5-fluorouracil (5-FU) susceptibility and side effect in patients with colon cancer.
  
  
  METHODS
  The total DNA of peripheral mononuclear cells was extracted in 100 cases of colon cancer patients. Quantitative PCR was conducted to measure DPYD gene 14G1A, A1627G, T85C 3 loci polymorphism, and analyze the correlation of 5-FU susceptibility, side effects with DPYD gene polymorphism.
  
  
  RESULTS
  Mutations were detected at position 14G1A (mutation rate 14%), A1627G (mutation rate 11%), and T85C (mutation rate 17%). The effective rate of 3 loci of wild type was significantly higher than that of mutant type (P < 0.05). With respect to side effects such as myelosuppression, hand-foot syndrome, diarrhea, and gastrointestinal reactions, the incidence in mutation type was significantly higher than that in the wild type (P < 0.05).
  
  
  CONCLUSIONS
  DPYD gene polymorphism plays a guiding role in predicting efficacy and toxicity of 5-FU, which can be used as an important reference index of 5-FU individualized administration scheme.
authors: Department of Surgical Oncology, Fuyang People's Hospital Fuyang, China.
authors: Wenhui
authors: W
authors: Sun
authors: Department of Gastroenterology, Affiliated Hospital of Shaoxing University Shaoxing 312000, Zhejiang, China.
authors: Chungen
authors: C
authors: Yan
authors: Department of Biotherapy, The 81st Hospital of PLA & Jingdu Hospital Nanjing, China.
authors: Shaochang
authors: S
authors: Jia
authors: Department of Biotherapy, The 81st Hospital of PLA & Jingdu Hospital Nanjing, China.
authors: Jianhua
authors: J
authors: Hu
keywords: 5-fluorouracil; Colon cancer; dihydropyrimidine dehydrogenase; gene polymorphism