title: Proteomics of protein post-translational modifications implicated in neurodegeneration.
abstract: Mass spectrometry (MS)-based proteomics has developed into a battery of approaches that is exceedingly adept at identifying with high mass accuracy and precision any of the following: oxidative damage to proteins (redox proteomics), phosphorylation (phosphoproteomics), ubiquitination (diglycine remnant proteomics), protein fragmentation (degradomics), and other posttranslational modifications (PTMs). Many studies have linked these PTMs to pathogenic mechanisms of neurodegeneration. To date, identifying PTMs on specific pathology-associated proteins has proven to be a valuable step in the evaluation of functional alteration of proteins and also elucidates biochemical and structural explanations for possible pathophysiological mechanisms of neurodegenerative diseases. This review provides an overview of methods applicable to the identification and quantification of PTMs on proteins and enumerates historic, recent, and potential future research endeavours in the field of proteomics furthering the understanding of PTM roles in the pathogenesis of neurodegeneration.
authors: Department of Neurology,Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.
authors: Ru-Jing
authors: RJ
authors: Ren
authors: Department of Biochemistry, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.
authors: Eric B
authors: EB
authors: Dammer
authors: Department of Pharmacology, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.
authors: Gang
authors: G
authors: Wang
authors: Department of Neurology,Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.|Department of Biochemistry, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.|Emory Proteomics Service Center, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.
authors: Nicholas T
authors: NT
authors: Seyfried
authors: Department of Neurology,Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322 USA.
authors: Allan I
authors: AI
authors: Levey
keywords: Alzheimer’s disease; Neurodegeneration; Parkinson’s disease; Protein posttranslational modifications; Proteomics