title: Intrinsic enantioselectivity of natural polynucleotides modulated by copper ions.
abstract: Natural polynucleotides including Micrococcus lysodeikticus and calf thymus DNA exhibit enantioselective recognition to S-ofloxacin regulated by Cu(2+). This is the first report that ofloxacin and Cu(2+) have cooperative effects on the local distortions of polynucleotides. At the [Cu(2+)]/[base] ratio of 0.1, S-ofloxacin is more liable to induce the locally distorted structures of polynucleotides, of which the association constant of S-ofloxacin toward DNA-Cu(II) is three times higher than that of the R-enantiomer. The apparent increase of adsorption capability and cooperativity, as well as the change of adsorption mechanism were detected in the adsorption of ofloxacin enantiomers on polynucleotides upon Cu(II)-coordination. This study not only discloses the effect of the chiral drug on the structural transition of long double-stranded DNA, but provides fundamental data to develop a novel enantioseparation method based on natural polynucleotides.
authors: Key Laboratory of Systems Bioengineering MOE, Key Laboratory for Green Chemical Technology MOE, Tianjin University; Collaborative Innovation Center of Chemical Science and Chemical Engineering, Tianjin, People's Republic of China.
authors: Yan
authors: Y
authors: Fu
authors: Xiongfei
authors: X
authors: Chen
authors: Jinli
authors: J
authors: Zhang
authors: Wei
authors: W
authors: Li
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D008837:Micrococcus; D015242:Ofloxacin / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D004269:DNA, Bacterial; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D015242:Ofloxacin
keywords: DNA; adsorption; chirality; copper; enantioseparation; ofloxacin