title: Detection of novel polyomaviruses in fruit bats in Indonesia.
abstract: Bats are an important natural reservoir for a variety of viral pathogens, including polyomaviruses (PyVs). The aims of this study were: (i) to determine which PyVs are present in bats in Indonesia and (ii) to analyze the evolutionary relationships between bat PyVs and other known PyVs. Using broad-spectrum polymerase chain reaction (PCR)-based assays, we screened PyV DNA isolated from spleen samples from 82 wild fruit bats captured in Indonesia. Fragments of the PyV genome were detected in 10 of the 82 spleen samples screened, and eight full-length viral genome sequences were obtained using an inverse PCR method. A phylogenetic analysis of eight whole viral genome sequences showed that BatPyVs form two distinct genetic clusters within the proposed genus Orthopolyomavirus that are genetically different from previously described BatPyVs. Interestingly, one group of BatPyVs is genetically related to the primate PyVs, including human PyV9 and trichodysplasia spinulosa-associated PyV. This study has identified the presence of novel PyVs in fruit bats in Indonesia and provides genetic information about these BatPyVs.
authors: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20 W10, Kita-ku, Sapporo, 001-0020, Japan.
authors: Shintaro
authors: S
authors: Kobayashi
authors: Michihito
authors: M
authors: Sasaki
authors: Ryo
authors: R
authors: Nakao
authors: Agus
authors: A
authors: Setiyono
authors: Ekowati
authors: E
authors: Handharyani
authors: Yasuko
authors: Y
authors: Orba
authors: Ibnu
authors: I
authors: Rahmadani
authors: Siswatiana
authors: S
authors: Taha
authors: Sri
authors: S
authors: Adiani
authors: Mawar
authors: M
authors: Subangkit
authors: Ichiro
authors: I
authors: Nakamura
authors: Takashi
authors: T
authors: Kimura
authors: Hirofumi
authors: H
authors: Sawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D004197:Disease Reservoirs / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D007214:Indonesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013154:Spleen / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins