title: Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences.
abstract: Taenia saginata is an important tapeworm, infecting humans in many parts of the world. The present study was undertaken to identify inter- and intraspecific variation of T. saginata isolated from cattle in different parts of Iran using two mitochondrial CO1 and 12S rRNA genes. Up to 105 bovine specimens of T. saginata were collected from 20 slaughterhouses in three provinces of Iran. DNA were extracted from the metacestode Cysticercus bovis. After PCR amplification, sequencing of CO1 and 12S rRNA genes were carried out and two phylogenetic analyses of the sequence data were generated by Bayesian inference on CO1 and 12S rRNA sequences. Sequence analyses of CO1 and 12S rRNA genes showed 11 and 29 representative profiles respectively. The level of pairwise nucleotide variation between individual haplotypes of CO1 gene was 0.3-2.4% while the overall nucleotide variation among all 11 haplotypes was 4.6%. For 12S rRNA sequence data, level of pairwise nucleotide variation was 0.2-2.5% and the overall nucleotide variation was determined as 5.8% among 29 haplotypes of 12S rRNA gene. Considerable genetic diversity was found in both mitochondrial genes particularly in 12S rRNA gene.
authors: Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, 76169-14111, Iran.
authors: Sima
authors: S
authors: Rostami
authors: Reza
authors: R
authors: Salavati
authors: Robin N
authors: RN
authors: Beech
authors: Zahra
authors: Z
authors: Babaei
authors: Mitra
authors: M
authors: Sharbatkhori
authors: Majid Fasihi
authors: MF
authors: Harandi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D041202:Taenia saginata / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013622:Taeniasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D015801:Helminth Proteins; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D003576:Electron Transport Complex IV