title: Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India.
abstract: This article describes the isolation and identification of contagious pustular dermatitis virus/orf virus (ORFV) from an outbreak of contagious pustular dermatitis (orf) in flocks of goats, in the north western region of India (Rajasthan). The virus was isolated in Vero cell cultures from scab and swab suspensions and has been identified using GIF/IL-2 and B2L gene specific primers in PCR and sequencing. The virus showed high nucleotide identity with previously reported Chinese, far eastern, Brazilian and Indian isolates. This report described the use of molecular tools for fast, reliable and confirmatory diagnosis of ORFV infection.
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Sushila
authors: S
authors: Maan
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Aman
authors: A
authors: Kumar
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Kanisht
authors: K
authors: Batra
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Mahendra
authors: M
authors: Singh
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Trilok
authors: T
authors: Nanda
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Arnab
authors: A
authors: Ghosh
authors: Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004 Haryana India.
authors: Narender S
authors: NS
authors: Maan
keywords: CPD; Contagious pustular dermatitis; Epidemiology; India; OPD; ORF; Orf; Ovine pustular dermatitis; Parapoxvirus; Poxviridae; Rajasthan