title: Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena.
abstract: Solanum melongena (eggplant), a member of the Solanaceae family, is a widely cultivated vegetable crop and is commonly used as a food throughout the world. Allergic reactions caused by members of this family are well known. However, mechanistic analyses to understand their molecular basis have not been adequately explored. In order to address this issue, the 7S vicilin protein (SM80.1) of size 45 kDa was purified from seeds of S. melongena by ammonium sulfate fractionation and size-exclusion chromatography. Significant homology of SM80.1 to an allergy-related protein from S. lycopersicum was identified through a BLAST search. Crystallization attempts with purified protein using the hanging-drop vapour-diffusion method led to hexagonal-shaped crystals. The crystals diffracted to 2.21 Å resolution and belonged to space group P6322, with unit-cell parameters a = 117.9, c = 123.5 Å.
authors: Regional Centre for Biotechnology, 180 Udyog Vihar Phase 1, Gurgaon, Haryana 122 016, India.
authors: Abha
authors: A
authors: Jain
authors: Regional Centre for Biotechnology, 180 Udyog Vihar Phase 1, Gurgaon, Haryana 122 016, India.
authors: Dinakar Masanu
authors: DM
authors: Salunke
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D032323:Solanum melongena / Q000737:chemistry*
chemical_list: D000485:Allergens; D010940:Plant Proteins
keywords: Solanaceae; Solanum melongena; allergy