title: Anti-biofilm and cytotoxicity activity of impregnated dressings with silver nanoparticles.
abstract: Infections arising from bacterial adhesion and colonization on chronic wounds are a significant healthcare problem. Silver nanoparticles (AgNPs) impregnated in dressing have attracted a great deal of attention as a potential solution. The goal of the present study was to evaluate the anti-biofilm activities of AgNPs impregnated in commercial dressings against Pseudomonas aeruginosa, bacteria isolated of chronic wounds from a hospital patient. The antimicrobial activity of AgNPs was tested within biofilms generated under slow fluid shear conditions using a standard bioreactor. A 2-log reduction in the number of colony-forming units of P. aeruginosa was recorded in the reactor on exposure to dressing impregnated with 250ppm of AgNPs, diameter 9.3±1.1nm, and also showed compatibility to mammalian cells (human fibroblasts). Our study suggests that the use of dressings with AgNPs may either prevent or reduce microbial growth in the wound environment, and reducing wound bioburden may improve wound-healing outcomes.
authors: Laboratorio de Microbiología, Facultad de Ciencias Químicas, UASLP, Mexico.
authors: Jorge Luis
authors: JL
authors: Velázquez-Velázquez
authors: Servicio de Epidemiología del Hospital Central "Dr. Ignacio Morones Prieto", San Luis Potosí, Mexico.
authors: Andrés
authors: A
authors: Santos-Flores
authors: Servicio de Epidemiología del Hospital Central "Dr. Ignacio Morones Prieto", San Luis Potosí, Mexico.
authors: Javier
authors: J
authors: Araujo-Meléndez
authors: Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación, Mexico.
authors: Roberto
authors: R
authors: Sánchez-Sánchez
authors: Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación, Mexico.
authors: Cristina
authors: C
authors: Velasquillo
authors: Laboratorio de Fisiología Celular, Facultad de Ciencias Químicas, UASLP, Mexico.
authors: Carmen
authors: C
authors: González
authors: Maestría en Ciencias Odontológicas Facultad de Estomatología, UASLP, Mexico.
authors: Gabriel
authors: G
authors: Martínez-Castañon
authors: Laboratorio de Microbiología, Facultad de Ciencias Químicas, UASLP, Mexico. Electronic address: fidel@uaslp.mx.
authors: Fidel
authors: F
authors: Martinez-Gutierrez
mesh_terms: D001458:Bandages; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000494:pharmacology*; D018030:Silver Compounds / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy
chemical_list: D018030:Silver Compounds; D012834:Silver
keywords: Anti-biofilm activity; Chronic wound; Dressing sample; Log reduction; Pseudomonas aeruginosa; Silver nanoparticles