title: Co-circulation in a single biome of the Juquitiba and Araraquara hantavirus detected in human sera in a sub-tropical region of Brazil.
abstract: Hantaviruses is an emerging infectious disease. Although HCPS has been reported in several regions of Brazil, more cases of HCPS have recently been reported in Minas Gerais than in any other state. In 2009, we analyzed 27 samples presenting antibodies against hantaviruses. These samples originated from 688 symptomatic patients, as determined based on the Hemorrhagic Fever Protocol. A subsequent SYBR Green-based real-time RT-PCR demonstrated the presence of the virus in 22 of the samples. Among the RT-PCR-positive samples, 17 were analyzed using DNA sequencing; these sequences were compared with others deposited in GenBank and showed similarity with the Araraquara and Juquitiba virus clusters. This work describe the detection of Juquitiba virus, including three fatal cases, in Minas Gerais state, furthermore, showed that it is feasible to characterize the circulating strains using a small fragment of S segment. Finally, the results suggest the co-circulation of Araraquara and Juquitiba virus in a single biome in Minas Gerais state.
authors: BSL3+ Clinical and Molecular Virology Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo (USP), São Paulo, Brazil.
authors: Jansen
authors: J
authors: de Araujo
authors: Ana I L
authors: AI
authors: Duré
authors: Raquel
authors: R
authors: Negrão
authors: Tatiana
authors: T
authors: Ometto
authors: Luciano M
authors: LM
authors: Thomazelli
authors: Edison Luiz
authors: EL
authors: Durigon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D002648:Child; D016000:Cluster Analysis; D005260:Female; D006476:Orthohantavirus / Q000145:classification* / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D055815:Young Adult
chemical_list: D012367:RNA, Viral
keywords: Araraquara virus; Juquitiba virus; SYBR Green real-time RT-PCR; fatal cases; human hantavirus