title: Transcriptome analysis in Cucumis sativus identifies genes involved in multicellular trichome development.
abstract: The regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been studied intensively, but that of multicellular remains unclear. In the present study, we characterized cucumber trichomes as representative multicellular and unbranched structures, but in a spontaneous mutant, mict (micro-trichome), all trichomes showed a micro-size and stunted morphologies. We revealed the transcriptome profile using Illumina HiSeq 2000 sequencing technology, and determined that a total of 1391 genes exhibited differential expression. We further validated the accuracy of the transcriptome data by RT-qPCR and found that 43 genes encoding critical transcription factors were likely involved in multicellular trichome development. These 43 candidate genes were subdivided into seven groups: homeodomain, MYB-domain, WRKY-domain, bHLH-domain, ethylene-responsive, zinc finger and other transcription factor genes. Our findings also serve as a powerful tool to further study the relevant molecular networks, and provide a new perspective for investigating this complex and species-specific developmental process.
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Jun-Long
authors: JL
authors: Zhao
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Jun-Song
authors: JS
authors: Pan
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Yuan
authors: Y
authors: Guan
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Jing-Tao
authors: JT
authors: Nie
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Jun-Jun
authors: JJ
authors: Yang
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Mei-Ling
authors: ML
authors: Qu
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
authors: Huan-Le
authors: HL
authors: He
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China. Electronic address: cairun@sjtu.edu.cn.
authors: Run
authors: R
authors: Cai
mesh_terms: D018553:Cucumis sativus / Q000378:metabolism*; D020869:Gene Expression Profiling; D064201:Trichomes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
keywords: Cucumis sativus; DNA-binding proteins; Gene regulatory networks; Transcription factors; Transcriptome; Trichomes