title: Titania nanotube arrays as interfaces for neural prostheses.
abstract: Neural prostheses have become ever more acceptable treatments for many different types of neurological damage and disease. Here we investigate the use of two different morphologies of titania nanotube arrays as interfaces to advance the longevity and effectiveness of these prostheses. The nanotube arrays were characterized for their nanotopography, crystallinity, conductivity, wettability, surface mechanical properties and adsorption of key proteins: fibrinogen, albumin and laminin. The loosely packed nanotube arrays fabricated using a diethylene glycol based electrolyte, contained a higher presence of the anatase crystal phase and were subsequently more conductive. These arrays yielded surfaces with higher wettability and lower modulus than the densely packed nanotube arrays fabricated using water based electrolyte. Further the adhesion, proliferation and differentiation of the C17.2 neural stem cell line was investigated on the nanotube arrays. The proliferation ratio of the cells as well as the level of neuronal differentiation was seen to increase on the loosely packed arrays. The results indicate that loosely packed nanotube arrays similar to the ones produced here with a DEG based electrolyte, may provide a favorable template for growth and maintenance of C17.2 neural stem cell line.
authors: Department of Mechanical Engineering, Colorado State University, Fort Collins CO 80523, USA.
authors: Jonathan A
authors: JA
authors: Sorkin
authors: Department of Chemical and Biological Engineering, Colorado State University, Fort Collins CO 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA.
authors: Stephen
authors: S
authors: Hughes
authors: Department of Mechanical Engineering, Polytechnic School, Pontifícia Universidade Católica do Paraná, Curitiba, PR 80215-901, Brazil.
authors: Paulo
authors: P
authors: Soares
authors: Department of Mechanical Engineering, Colorado State University, Fort Collins CO 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA. Electronic address: ketul.popat@colostate.edu.
authors: Ketul C
authors: KC
authors: Popat
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D043942:Nanotubes / Q000737:chemistry*; D058117:Neural Prostheses; D058953:Neural Stem Cells / Q000187:drug effects; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D017155:Wettability / Q000187:drug effects
chemical_list: D007797:Laminin; C009495:titanium dioxide; D014025:Titanium
keywords: Astrocytes; C17.2 neural stem cell line; Neural prostheses; Neurons; Titania nanotube arrays