title: Amniotic membrane as a biological dressing for 5-fluoruracil-induced oral mucositis in rats.
abstract: Oral mucositis is a reaction to chemoradiation therapy during cancer treatment. The aim of this study was to investigate the use of amniotic membrane as a biological dressing for oral mucositis lesions in rats. Sixty Wistar rats were divided into three groups (n = 20): control, 5-fluoruracil (5-FU), 5-fluoruracil + amniotic membrane (5-FU+AM). Each group was subdivided (n = 5) according to the time interval to sacrifice (3, 7, 14, and 21 days). Histology (haematoxylin-eosin staining) and immunocytochemistry (anti-rat antibodies CD4, CD8, VEGF, and PCNA) were evaluated. Immunocytochemistry results were analyzed using one-way ANOVA and Tukey tests. The amniotic membrane (5FU+AM) played an important role in cell proliferation (PCNA 3 days 27.08 ± 4.65, 7 days 27.90 ± 3.34) and especially in neovascularization (VEGF 3 days 23.00 ± 1.40, 7 days 26.00 ± 0.95) for all time intervals, when compared to 5-FU (PCNA 3 days 23.12 ± 1.61, 7 days 37.21 ± 1.20; VEGF 3 days 17.05 ± 1.51, 7 days 8.45 ± 1.35) and control (PCNA 3 days 29.99 ± 0.92, 7 days 16.33 ± 2.88; VEGF 3 days 13.65 ± 0.55, 7 days 15.70 ± 1.39). It was biocompatible, showing significant differences compared to the other groups in CD4 (F = 40.72; P = 0.001) and CD8 (F = 69.99, P = 0.001) staining together, only during the inflammation phase (7 days). Amniotic membrane presented biocompatibility and stimulated cell proliferation and neovascularization, functioning as a promising biological dressing.
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil. Electronic address: gabrielademorais@yahoo.com.br.
authors: G de M G
authors: Gde M
authors: Lima
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: M C
authors: MC
authors: Severo
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: G de F
authors: Gde F
authors: Santana-Melo
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: M A
authors: MA
authors: Carvalho
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: M das G
authors: Md
authors: Vilela-Goulart
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: M A C
authors: MA
authors: Salgado
authors: Department of Bioscience and Oral Diagnosis, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José dos Campos, São Paulo, Brazil.
authors: M F
authors: MF
authors: Gomes
mesh_terms: D000650:Amnion; D000818:Animals; D001684:Biological Dressings; D015415:Biomarkers / Q000032:analysis; D018791:CD4 Lymphocyte Count; D016827:CD8 Antigens / Q000032:analysis; D049109:Cell Proliferation; D005472:Fluorouracil / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D018919:Neovascularization, Physiologic; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013280:Stomatitis / Q000139:chemically induced* / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D016827:CD8 Antigens; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D005472:Fluorouracil
keywords: amniotic membrane; chemotherapy; oral mucositis; therapy; wound healing