title: Triterpenoid saponins from Albizia lebbeck (L.) Benth and their inhibitory effect on the survival of high grade human brain tumor cells.
abstract: As part of our search of new bioactive triterpenoid saponins from Cameroonian Mimosaceae plants, phytochemical investigation of the roots of Albizia lebbeck led to the isolation of two new oleanane-type saponins, named lebbeckosides A-B (1-2). Their structures were established on the basis of extensive 1D and 2D NMR ((1)H, (13)C NMR, DEPT, COSY, TOCSY, ROESY, HSQC, and HMBC) and HRESIMS studies, and by chemical evidence. Compounds 1-2 were evaluated for their inhibitory effect on the metabolism of high grade human brain tumor cells, the human glioblastoma U-87 MG cell lines and the glioblastoma stem-like TG1 cells isolated from a patient tumor, and known to be particularly resistant to standard therapies. The isolated saponins showed significant cytotoxic activity against U-87 MG and TG1 cancer cells with IC50 values of 3.46 μM and 1.36 μM for 1, and 2.10 μM and 2.24 μM for 2, respectively.
authors: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé, BP 812 Yaoundé, Cameroon. Electronic address: oliviernote1@yahoo.fr.
authors: Olivier Placide
authors: OP
authors: Noté
authors: Chimie-Biologie Intégrative, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors: Dong
authors: D
authors: Jihu
authors: Service Commun d'Analyse, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors: Cyril
authors: C
authors: Antheaume
authors: Chimie-Biologie Intégrative, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors: Maria
authors: M
authors: Zeniou
authors: Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé, BP 812 Yaoundé, Cameroon.
authors: Dieudonné Emmanuel
authors: DE
authors: Pegnyemb
authors: UFR Médecine-Pharmacie, CNRS-UMR7312, 51 rue Cognacq Jay, 51100 Reims, France.
authors: Dominique
authors: D
authors: Guillaume
authors: Neuroscience Paris Seine, IBPS, CNRS-UMR 8246, Inserm U1130, UPMC, 7 quai Saint Bernard, 75005 Paris, France.
authors: Hervé
authors: H
authors: Chneiwess
authors: Chimie-Biologie Intégrative, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors: Marie Claude
authors: MC
authors: Kilhoffer
authors: Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire d'Innovation Thérapeutique, UMR 7200, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Cedex, France.
authors: Annelise
authors: A
authors: Lobstein
mesh_terms: D031289:Albizzia / Q000737:chemistry; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C000603172:lebbeckoside A; C000603173:lebbeckoside B
keywords: Albizia lebbeck; Cytotoxic activity; Lebbeckosides; Mimosaceae; Triterpenoid saponins