title: CD147 modulates autophagy through the PI3K/Akt/mTOR pathway in human prostate cancer PC-3 cells.
abstract: The multifunctional glycoprotein cluster of differentiation (CD)147 is highly expressed on the cell surface of the majority of cancer cells, and promotes tumor invasion, metastasis and growth. However, the role of CD147 in autophagy has not yet been explored in prostrate cancer cells. In the present study, prostate cancer PC-3 cells were cultured under starvation conditions, and the expression level of CD147 gradually increased. Therefore, RNA interference was used to inhibit CD147 expression, in order to investigate the biological role of this glycoprotein in autophagy progression. Autophagic activity was monitored by the changes in green fluorescent protein-light chain 3 (GFP-LC3) location and the expression of the autophagy-associated protein LC3-II. It was found that downregulation of CD147 significantly promoted GFP-LC3 puncta formation and the expression of LC3-II. Furthermore, the levels of phosphorylated serine/threonine protein kinase B (p-Akt) and phosphorylated mammalian target of rapamycin (p-mTOR) were significantly decreased, and the level of LC3-II was inversely associated with levels of p-Akt and p-mTOR in cells with downregulated expression of CD147. The results of a trypan blue exclusion assay revealed that starvation-induced cell death was increased in PC-3/shCD147 cells compared with control PC-3/Scramble cells (37.7±6.4 vs. 21.7±5.5%). Together, these results indicate that CD147 may be important in the inhibition of autophagy via the PI3K/Akt/mTOR pathway, which prevents cell death from unrestrained autophagy.
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Fang
authors: F
authors: Fang
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Liuhang
authors: L
authors: Wang
authors: Department of Biochemistry, Changchun Medical College, Changchun, Jilin 130021, P.R. China.
authors: Shufang
authors: S
authors: Zhang
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Qing
authors: Q
authors: Fang
authors: Department of Biochemistry, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Feng
authors: F
authors: Hao
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Yanmei
authors: Y
authors: Sun
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Liangzhong
authors: L
authors: Zhao
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Shuang
authors: S
authors: Chen
authors: Department of Immunology, Jilin Medical College, Jilin, Jilin 132013, P.R. China.
authors: Huijuan
authors: H
authors: Liao
authors: Department of Urology, Affiliated Hospital of Jilin Medical College, Jilin, Jilin 132011, P.R. China.
authors: Liguo
authors: L
authors: Wang
keywords: PI3K/Akt/mTOR pathway; autophagy; cluster of differentiation 147; prostate cancer