title: New pregnane glycosides from Gymnema sylvestre.
abstract: Four new pregnane glycosides 1-4 were isolated from the ethanol extract of the stem of Gymnema sylvestre and named gymsylvestrosides A-D. Hydrolysis of compound 1 under the catalysis of Aspergilus niger β-glucosidase afforded compound 5 (gymsylvestroside E). Their structures were determined by spectroscopic methods such as HRESIMS, 1D and 2D NMR, as well as HMQC-TOCSY experiment. Compounds 1-4 were screened for Saccharomyces cerevisiae α-glucosidase inhibitory activity.
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. xurui2015@163.com.
authors: Rui
authors: R
authors: Xu
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. tommase2015@163.com.
authors: Yu
authors: Y
authors: Yang
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. zyqci2015@163.com.
authors: Yang
authors: Y
authors: Zhang
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. ren2015victory@163.com.
authors: Fengxia
authors: F
authors: Ren
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. xujinlong2015@163.com.
authors: Jinlong
authors: J
authors: Xu
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. nengjiang_yu@163.com.
authors: Nengjiang
authors: N
authors: Yu
authors: Institute of Pharmacology and Toxicology, Academy for Military Medical Science, 27 Taiping Road, Haidian District, Beijing 100850, China. zhaoymamms@163.com.
authors: Yimin
authors: Y
authors: Zhao
mesh_terms: D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D031104:Gymnema sylvestre / Q000737:chemistry*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D006027:Glycosides; D011278:Pregnanes; D029701:Saccharomyces cerevisiae Proteins; D000520:alpha-Glucosidases