title: Antioxidant activity of humic substances via bioluminescent monitoring in vitro.
abstract: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K3[Fe(СN)6] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by
  FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D GT and D OxT, respectively. Dependencies of D GT and D OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5 × 10(-4) to 2 × 10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.
authors: Siberian Federal University, Krasnoyarsk, Russia, 660041, as421@yandex.ru.
authors: A S
authors: AS
authors: Tarasova
authors: D I
authors: DI
authors: Stom
authors: N S
authors: NS
authors: Kudryasheva
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D016227:Benzoquinones; D004784:Environmental Monitoring / Q000379:methods*; D038181:FMN Reductase / Q000378:metabolism; D006812:Humic Substances / Q000032:analysis*; D066298:In Vitro Techniques; D008156:Luciferases; D008163:Luminescent Measurements / Q000379:methods; D010084:Oxidation-Reduction; D012492:Salts; D012988:Soil Microbiology
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D006812:Humic Substances; D012492:Salts; C004532:quinone; D008156:Luciferases; C071832:alkanol monooxygenase (FMN-linked); D038181:FMN Reductase