title: Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture.
abstract: L-DOPA (3,4-dihydroxyphenyl-L-alanine), a modified amino acid, is an expansively used drug for the Parkinson's disease treatment. In the present study, optimization of nutritional parameters influencing L-DOPA production was attempted using the response surface methodology (RSM) from Mucuna monosperma callus. Optimization of the four factors was carried out using the Box-Behnken design. The optimized levels of factors predicted by the model include tyrosine 0.894 g l(-1), pH 4.99, ascorbic acid 31.62 mg l(-1)and copper sulphate 23.92 mg l(-1), which resulted in highest L-DOPA yield of 0.309 g l(-1). The optimization of medium using RSM resulted in a 3.45-fold increase in the yield of L-DOPA. The ANOVA analysis showed a significant R (2) value (0.9912), model F-value (112.465) and probability (0.0001), with insignificant lack of fit. Optimized medium was used in the laboratory scale column reactor for continuous production of L-DOPA. Uninterrupted flow column exhibited maximum L-DOPA production rate of 200 mg L(-1) h(-1) which is one of the highest values ever reported using plant as a biotransformation source. L-DOPA production was confirmed by HPTLC and HPLC analysis. This study demonstrates the synthesis of L- DOPA using Mucuna monosperma callus using a laboratory scale column reactor.
authors: Department of Biotechnology, Shivaji University, Kolhapur, 416 004 India.
authors: Shrirang Appasaheb
authors: SA
authors: Inamdar
authors: Department of Microbiology, Shivaji University, Kolhapur, 416 004 India.
authors: Shripad Nagnath
authors: SN
authors: Surwase
authors: Department of Biochemistry, Shivaji University, Kolhapur, 416 004 India.
authors: Shekhar Bhagwan
authors: SB
authors: Jadhav
authors: Department of Biotechnology, Shivaji University, Kolhapur, 416 004 India.
authors: Vishwas Anant
authors: VA
authors: Bapat
authors: Department of Biotechnology, Shivaji University, Kolhapur, 416 004 India ; Department of Biochemistry, Shivaji University, Kolhapur, 416 004 India.
authors: Jyoti Prafull
authors: JP
authors: Jadhav
keywords: Biotransformation; Continuous culture; L-DOPA; Mucuna monosperma; Response surface methodology