title: Decreased expression of SCUBE2 is associated with progression and prognosis in colorectal cancer.
abstract: Signal peptide-CUB-epidermal growth factor-like domain-containing protein 2 (SCUBE2), a member of the SCUBE protein family, is a secreted and membrane-associated multi-domain glycoprotein. SCUBE2 is known as a novel tumor suppressor and a useful prognostic marker in breast cancer. In the present study, we investigated the expression (including mRNA and protein levels) of SCUBE2 in colorectal cancer (CRC) and adjacent normal tissues, using quantitative real-time PCR, western blot analysis and immunohistochemistry on a tissue microarray. Upregulation of SCUBE2 was achieved by transient transfection in RKO cell lines, and the effects of SCUBE2 on tumor proliferation, invasion, migration and apoptosis were evaluated by a series of functional experiments. The results indicated that SCUBE2 expression was decreased at the transcriptional and translational levels in CRC tissues and significantly associated with clinical stage, the depth of tumor invasion, lymph‑node metastasis, distant metastasis and histological grade. Patients with SCUBE2‑positive tumors had a lower recurrence rate and better survival than patients with SCUBE2-negative tumors. Moreover, upregulation of SCUBE2 had a limited effect on cell apoptosis but significantly inhibited tumor cell proliferation, migration and invasion in vitro. In conclusion, SCUBE2 plays an important role in suppressing CRC progression and prognosis. Our findings suggested that SCUBE2 may serve as a novel tumor suppressor and a potential therapeutic target for CRC patients.
authors: Department of Pathology, Taishan Medical University, Taian, Shandong, P.R. China.
authors: Qi
authors: Q
authors: Song
authors: Department of Pathology, Taishan Medical University, Taian, Shandong, P.R. China.
authors: Chuanwei
authors: C
authors: Li
authors: Department of Pathology, Taishan Medical University, Taian, Shandong, P.R. China.
authors: Xiaodong
authors: X
authors: Feng
authors: Department of Etiology, Taishan Medical University, Taian, Shandong, P.R. China.
authors: Ailian
authors: A
authors: Yu
authors: Department of Pathology, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, P.R. China.
authors: Huamei
authors: H
authors: Tang
authors: Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, P.R. China.
authors: Zhihai
authors: Z
authors: Peng
authors: Department of Pathology, Taishan Medical University, Taian, Shandong, P.R. China.
authors: Xuechun
authors: X
authors: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002135:Calcium-Binding Proteins; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014410:Tumor Stem Cell Assay; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011993:Recombinant Fusion Proteins; C469999:SCUBE2 protein, human; D025521:Tumor Suppressor Proteins