title: Heterologous expression of IbMYB1a by different promoters exhibits different patterns of anthocyanin accumulation in tobacco.
abstract: We previously reported that the transient and stable expression of IbMYB1a produced anthocyanin pigmentation in tobacco leaves and transgenic Arabidopsis plants, respectively. To further determine the effects of different promoters on the expression of IbMYB1a and anthocyanin production, we generated and characterized stably transformed tobacco (Nicotiana tabacum SR1) plants expressing IbMYB1a under the control of three different promoters. We compared the differences in anthocyanin accumulation patterns and phenotypic features of the leaves of these transgenic tobacco plants during growth. Expression of IbMYB1a under the control of these three different promoters led to a remarkable variation in anthocyanin pigmentation in tobacco leaves. The anthocyanin contents of the leaves of the SPO-IbMYB1a-OX (SPO-M) line were higher than those of the SWPA2-IbMYB1a-OX (SPA-M) and 35S-IbMYB1a-OX (35S-M) lines. High levels of anthocyanin pigments negatively affected plant growth in the SPO-M lines, resulting delayed growth and, occasionally, a stunted phenotype. Furthermore, HPLC analysis revealed that transcriptional regulation of IbMYB1a led to the production of cyanidin-based anthocyanins in the tobacco plants. In addition, RT-PCR analysis revealed that IbMYB1a expression induced the up-regulation of several structural genes in the anthocyanin biosynthetic pathway, including DFR and ANS. Differential expression levels of IbMYB1a under the control of different promoters were highly correlated with the expression levels of the structural genes, thereby affecting anthocyanin production levels. These results indicate that IbMYB1a positively controls the expression of multiple anthocyanin biosynthetic genes and anthocyanin accumulation in heterologous tobacco plants.
authors: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, Republic of Korea; Department of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-806, Republic of Korea.
authors: Chul Han
authors: CH
authors: An
authors: Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan 331-801, Republic of Korea.
authors: Ki-Won
authors: KW
authors: Lee
authors: Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan 331-801, Republic of Korea.
authors: Sang-Hoon
authors: SH
authors: Lee
authors: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, Republic of Korea.
authors: Yu Jeong
authors: YJ
authors: Jeong
authors: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, Republic of Korea.
authors: Su Gyoung
authors: SG
authors: Woo
authors: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, Republic of Korea.
authors: Hyokon
authors: H
authors: Chun
authors: Department of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-806, Republic of Korea.
authors: Youn-Il
authors: YI
authors: Park
authors: Plant Systems Engineering Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Sang-Soo
authors: SS
authors: Kwak
authors: Eco-friendly Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, Republic of Korea. Electronic address: kimcy@kribb.re.kr.
authors: Cha Young
authors: CY
authors: Kim
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D027723:Ipomoea batatas / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D014157:Transcription Factors; C017154:cyanidin
keywords: Anthocyanin; Promoter; R2R3-MYB transcription factor; Sweetpotato; Tobacco; Transgenic plants