title: Viral-mediated expression of c-Myc and cyclin A2 induces cochlear progenitor cell proliferation.
abstract: Cochlear progenitor cells have a limited proliferative capability, which prevents their application in treating sensorineural hearing loss. In this study, we showed that the expression of c-Myc and cyclin A2 was down-regulated during the development of cochlear tissue and CPC differentiation. Over-expression of these two genes using adenovirus transduction, significantly affected the CPC cell cycle and promoted the CPC proliferation. We further demonstrated that this promotion involves the classic CKI-cyclin-CDK pathway. Our study suggests that genetically modified CPCs may be a promising cell source for cochlear stem cell transplantation that improves the efficacy of cell therapy.
authors: Department of Otolaryngology, Lanzhou General Hospital of People's Liberation Army, 730050 Lanzhou, China.
authors: Cuiping
authors: C
authors: Zhong
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China.
authors: Yu
authors: Y
authors: Han
authors: Department of Breast Surgery, Lanzhou General Hospital of People's Liberation Army, 730050 Lanzhou, China.
authors: Ji
authors: J
authors: Ma
authors: Medical Office, Chinese People's Liberation Army General Hospital, 100039 Beijing, China.
authors: Xuan
authors: X
authors: Zhang
authors: Department of Clinical Laboratory, Hospital of the Armed Police Force of Gansu Province, 730050 Lanzhou, China.
authors: Mengning
authors: M
authors: Sun
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China.
authors: Ye
authors: Y
authors: Wang
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China.
authors: Jun
authors: J
authors: Chen
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China.
authors: Wenjuan
authors: W
authors: Mi
authors: Department of Otolaryngology, Lanzhou General Hospital of People's Liberation Army, 730050 Lanzhou, China. Electronic address: doctorzhong@hotmail.com.
authors: Xuehai
authors: X
authors: Xu
authors: Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, China. Electronic address: qiujh@fmmu.edu.cn.
authors: Jianhua
authors: J
authors: Qiu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation; D049109:Cell Proliferation; D003051:Cochlea / Q000166:cytology* / Q000378:metabolism; D056751:Cyclin A2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
chemical_list: C503721:CCNA2 protein, human; D056751:Cyclin A2; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc
keywords: Cell-cycle modulation; Cochlear progenitor cells; Proliferation