title: Galactose utilization sheds new light on sugar metabolism in the sequenced strain Dekkera bruxellensis CBS 2499.
abstract: Dekkera bruxellensis and Saccharomyces cerevisiae are considered two phylogenetically distant relatives, but they share several industrial relevant traits such as the ability to produce ethanol under aerobic conditions (Crabtree effect), high tolerance towards ethanol and acids, and ability to grow without oxygen. Beside a huge adaptability, D. bruxellensis exhibits a broader spectrum in utilization of carbon and nitrogen sources in comparison to S. cerevisiae. With the aim to better characterize its carbon source metabolism and regulation, the usage of galactose and the role that glucose plays on sugar metabolism were investigated in D. bruxellensis CBS 2499. The results indicate that in this yeast galactose is a non-fermentable carbon source, in contrast to S. cerevisiae that can ferment it. In particular, its metabolism is affected by the nitrogen source. Interestingly, D. bruxellensis CBS 2499 exhibits the 'short-term Crabtree effect', and the expression of genes involved in galactose utilization and in respiratory metabolism is repressed by glucose, similarly to what occurs in S. cerevisiae.
authors: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via G. Celoria 2, 20133, Italy.
authors: Md
authors: M
authors: Moktaduzzaman
authors: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via G. Celoria 2, 20133, Italy.
authors: Silvia
authors: S
authors: Galafassi
authors: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via G. Celoria 2, 20133, Italy.
authors: Claudia
authors: C
authors: Capusoni
authors: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via G. Celoria 2, 20133, Italy.
authors: Ileana
authors: I
authors: Vigentini
authors: Department of Biology, Lund University, Box 117, 221 00 Lund, Sweden.
authors: Zhihao
authors: Z
authors: Ling
authors: Department of Biology, Lund University, Box 117, 221 00 Lund, Sweden.
authors: Jure
authors: J
authors: Piškur
authors: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via G. Celoria 2, 20133, Italy concetta.compagno@unimi.it.
authors: Concetta
authors: C
authors: Compagno
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D055168:Brettanomyces / Q000235:genetics* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism
chemical_list: D000431:Ethanol; D002244:Carbon; D005947:Glucose; D009584:Nitrogen; D019342:Acetic Acid; D005690:Galactose
keywords: acetic acid production; carbon metabolism; ethanol production; glucose repression; nitrogen metabolism