title: Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds.
abstract: Apuleia leiocarpa is a tree found in Caatinga that has great value in the timber industry. Lectins are carbohydrate-binding proteins with several biotechnological applications. This study shows the isolation, characterization, and antibacterial activity of A. leiocarpa seed lectin (ApulSL). The lectin was chromatographically isolated from a crude extract (in 150 mM NaCl) by using a chitin column. ApulSL adsorbed to the matrix and was eluted using 1.0 M acetic acid. Native ApulSL was characterized as a 55.8-kDa acidic protein. SDS-PAGE showed three polypeptide bands, whereas two-dimensional electrophoresis revealed four spots. The peptides detected by MALDI TOF/TOF did not show sufficient homology (<30%) with the database proteins. Circular dichroism spectroscopy suggested a disordered conformational structure, and fluorescence spectrum showed the presence of tyrosine residues in the hydrophobic core. The hemagglutinating activity of ApulSL was present even after heating to 100 °C, was Mn(2+)-dependent, and inhibited by N-acetylglucosamine, D(-)-arabinose, and azocasein. ApulSL demonstrated bacteriostatic and bactericide effects on gram-positive and gram-negative species, being more effective against three varieties of Xanthomonas campestris (MIC ranging from 11.2 to 22.5 μg/mL and MBC of 22.5 μg/mL). The results of this study reinforce the importance of biochemical prospecting of Caatinga by revealing the antibacterial potential of ApulSL.
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Aline de Souza
authors: Ade S
authors: Carvalho
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Márcia Vanusa
authors: MV
authors: da Silva
authors: Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, 57072-900 Maceió, Alagoas, Brazil.
authors: Francis Soares
authors: FS
authors: Gomes
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Patrícia Maria Guedes
authors: PM
authors: Paiva
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Carolina Barbosa
authors: CB
authors: Malafaia
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Tulio Diego
authors: TD
authors: da Silva
authors: Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, Jatobá, 58700-970 Patos, Paraíba, Brazil.
authors: Antônio Fernando de Melo
authors: AF
authors: Vaz
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Alexandre Gomes
authors: AG
authors: da Silva
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Isabel Renata de Souza
authors: IR
authors: Arruda
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil. Electronic address: thiagohn86@yahoo.com.br.
authors: Thiago Henrique
authors: TH
authors: Napoleão
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Maria das Graças
authors: Md
authors: Carneiro-da-Cunha
authors: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors: Maria Tereza dos Santos
authors: MT
authors: Correia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D006388:Hemagglutinins / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D006388:Hemagglutinins; D010455:Peptides; D037121:Plant Lectins
keywords: Apuleia leiocarpa; Lectin; Xanthomonas campestris