title: An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform.
abstract: A novel amplified fluorescence graphene oxide (GO) sensing platform for sensitive detection of T4 polynucleotide kinase (PNK) activity and inhibition was developed based on the exonuclease III (ExoIII) reaction. The efficient digestion capacity of ExoIII and the super quenching ability of GO both contribute to the detection sensitivity.
authors: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu Shandong 273165, P. R. China. kongrongmei@126.com jmyou6304@163.com.
authors: Ni-Na
authors: NN
authors: Sun
authors: Rong-Mei
authors: RM
authors: Kong
authors: Fengli
authors: F
authors: Qu
authors: Xiaobing
authors: X
authors: Zhang
authors: Shufang
authors: S
authors: Zhang
authors: Jinmao
authors: J
authors: You
mesh_terms: D000818:Animals; D017122:Bacteriophage T4 / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D057075:Enzyme Assays / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D010087:Oxides / Q000737:chemistry; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D006108:Graphite; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III