title: Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae).
abstract: Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR. Vindogentianine is a dimer containing a vindoline moiety coupled to a gentianine moiety. After 24h incubation, vindogentianine exhibited no cytotoxic effect in C2C12 mouse myoblast and β-TC6 mouse pancreatic cells (IC50>50μg/mL). Real-time cell proliferation monitoring also indicated vindogentianine had little or no effect on C2C12 mouse myoblast cell growth at the highest dose tested (200μg/mL), without inducing cell death. Vindogentianine exhibited potential hypoglycemic activity in β-TC6 and C2C12 cells by inducing higher glucose uptake and significant in vitro PTP-1B inhibition. However, in vitro α-amylase and α-glucosidase inhibition assay showed low inhibition under treatment of vindogentianine. This suggests that hypoglycemic activity of vindogentianine may be due to the enhancement of glucose uptake and PTP-1B inhibition, implying its therapeutic potential against type 2 diabetes.
authors: Sime Darby Technology Centre, Serdang, Malaysia.
authors: Soon Huat
authors: SH
authors: Tiong
authors: Department of Pharmacology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Chung Yeng
authors: CY
authors: Looi
authors: Department of Pharmacy, University of Malaya, Kuala Lumpur, Malaysia.
authors: Aditya
authors: A
authors: Arya
authors: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Won Fen
authors: WF
authors: Wong
authors: Center for Natural Product and Drug Discovery (CENAR), University of Malaya, Kuala Lumpur, Malaysia.
authors: Hazrina
authors: H
authors: Hazni
authors: Department of Pharmacology, University of Malaya, Kuala Lumpur, Malaysia.
authors: Mohd Rais
authors: MR
authors: Mustafa
authors: Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia. Electronic address: khalijah@um.edu.my.
authors: Khalijah
authors: K
authors: Awang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D030008:Catharanthus / Q000737:chemistry*; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification; D026121:Indole Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000470:Alkaloids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D026121:Indole Alkaloids; D010936:Plant Extracts; C000598400:vindogentianine; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; C516609:Ptpn1 protein, mouse; D000516:alpha-Amylases; D005947:Glucose
keywords: Apocynaceae; Catharanthus roseus; Glucose uptake; Hypoglycemic; PTP-1B; Vindogentianine