title: Enhanced autotrophic astaxanthin production from Haematococcus pluvialis under high temperature via heat stress-driven Haber-Weiss reaction.
abstract: High temperatures (30-36 °C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O2 (-) and H2O2) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber-Weiss reaction to convert LROS into more reactive oxygen species (MROS; O2 and OH·), thereby facilitating lipid peroxidation. As a result, during 18 days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450 μM) was dramatically increased by 75 % (30 °C) and 133 % (36 °C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber-Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis.
authors: Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea.
authors: Min-Eui
authors: ME
authors: Hong
authors: Sung Kwan
authors: SK
authors: Hwang
authors: Won Seok
authors: WS
authors: Chang
authors: Byung Woo
authors: BW
authors: Kim
authors: Jeewon
authors: J
authors: Lee
authors: Sang Jun
authors: SJ
authors: Sim
mesh_terms: D052818:Autotrophic Processes / Q000528:radiation effects; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D006358:Hot Temperature; D008027:Light; D017382:Reactive Oxygen Species / Q000378:metabolism; D024341:Xanthophylls / Q000096:biosynthesis
chemical_list: D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine