title: Cystic fibrosis: model of pathogenesis based on the apical membrane potential.
abstract: A simple model of cystic fibrosis (CF) is proposed, based on the apical membrane (ApM) potential. The ApM of epithelial cells is highly permeable to sodium and activation of CFTRs makes it permeable to chloride. Calculated ApM potentials of cells with activated cystic fibrosis transmembrane conductance regulators (CFTRs) are between the sodium and chloride Nernst values and thus allow rapid absorption of both ions in exocrine glands. In CF patients the potential is near the sodium Nernst value and thus more salt is left in the ducts. Simulation predicts that the sodium driving force increases more than 3.5 times if the ApM permeability for Cl- increases from 5-94% of the sodium permeability. In pancreatic ductal cells basolateral sodium bicarbonate cotransporters (pNBC1) allows influx of bicarbonates with sodium. Bicarbonates are exchanged for intraductal chloride by anion exchanger 1 (AE1) in the ApM. Activated CFTRs let some chloride to leak back to ducts, followed by water that dilutes ductal proteins. Replenished intraductal chloride allows more bicarbonate secretion. In CF patients, pancreatic water and bicarbonate secretion is limited by the intraductal chloride pool.
authors: Department of Anesthesiology, Zagreb University Hospital, Zagreb; Croatia.
authors: Beatrica
authors: B
authors: Kurbel
authors: Department of Orthopedic Surgery, University Hospital, Osijek; Croatia.
authors: Saša
authors: S
authors: Rapan
authors: Department of Internal Medicine, University Hospital, Osijek; Croatia.
authors: Sven
authors: S
authors: Kurbel
mesh_terms: D003550:Cystic Fibrosis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D010183:Pancreatic Ducts / Q000503:physiopathology*; D010539:Permeability