title: Kinetics of lipogenic genes expression in milk purified mammary epithelial cells (MEC) across lactation and their correlation with milk and fat yield in buffalo.
abstract: Expression patterns of lipogenic genes (LPL, ABCG2, ACSS2, ACACA, SCD, BDH, LIPIN1, SREBF1, PPARα and PPARγ) were studied in milk purified MEC across different stages of lactation (15, 30, 45, 60, 90, 120 and 240 days relative to parturition) in buffalo. PPARα was the most abundant gene while ABCG2 and ACSS2 had moderate level of expression; whereas expression of SREBF and PPARγ was very low. The expression patterns of some genes (BDH1, ACSS2, and LIPIN1) across lactation were positively correlated with milk yield while negatively correlated with fat yield. SCD also showed weak correlation with milk yield (p, 0.53) and fat yield (p, -0.47). On the other hand, expression pattern of ACACA was negatively correlated with milk yield (p, -0.88) and positively correlated with fat yield (p, 0.62). Strong correlation was observed between genes involved in de novo milk fat synthesis (BDH1, ACSS2, LIPIN2 and SCD) and milk yield.
authors: National Bureau of Animal Genetic Resources, Karnal 132001, India; Department of Science and Technology, Goverment of India, New Delhi, India. Electronic address: niryadav@gmail.com.
authors: Poonam
authors: P
authors: Yadav
authors: National Dairy Research Institute, Karnal 132001, India.
authors: Parveen
authors: P
authors: Kumar
authors: National Bureau of Animal Genetic Resources, Karnal 132001, India.
authors: Manishi
authors: M
authors: Mukesh
authors: National Bureau of Animal Genetic Resources, Karnal 132001, India.
authors: R S
authors: RS
authors: Kataria
authors: Department of Biotechnology, Kurukshetra University, Kurukshetra 136119, India.
authors: Anita
authors: A
authors: Yadav
authors: National Dairy Research Institute, Karnal 132001, India.
authors: A K
authors: AK
authors: Mohanty
authors: National Bureau of Animal Genetic Resources, Karnal 132001, India.
authors: B P
authors: BP
authors: Mishra
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics* / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D005223:Fats / Q000032:analysis / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007700:Kinetics; D007774:Lactation / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005223:Fats
keywords: Buffalo; Lactation; Lipogenic genes; Mammary epithelial cells; Milk fat