title: Effect of kojic acid-grafted-chitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria.
abstract: Our work here, for the first time, reported the antibacterial activity of kojic acid-grafted-chitosan oligosaccharides (COS/KA) against three gram-positive and three gram-negative bacteria. Integrity of cell membrane, outer membrane (OM) and inner membrane (IM) permeabilization assay, alkaline phosphatase (ALP) and glucose-6-phosphate dehydrogenase (G6PDH) assay, and SDS-PAGE assay techniques were used to investigate the interactions between COS/KA and bacterial membranes. The antibacterial activity of COS/KA was higher than those of unmodified COS. The electric conductivity of bacteria suspensions increased, followed by increasing of the units of average release for ALP and G6PDH. COS/KA can also rapidly increase the 1-N-phenylanphthylamine (NPN) uptake and the release of β-galactosidase via increasing the permeability of OM and IM in Escherichia coli. SDS-PAGE indicated the content of cellular soluble proteins decreased significantly in COS/KA-treated bacteria. Hence, COS/KA has potential in food industry and biomedical sciences.
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, People's Republic of China.
authors: Xiaoli
authors: X
authors: Liu
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, People's Republic of China. Electronic address: xiaws@jiangnan.edu.cn.
authors: Wenshui
authors: W
authors: Xia
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, People's Republic of China.
authors: Qixing
authors: Q
authors: Jiang
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, People's Republic of China.
authors: Yanshun
authors: Y
authors: Xu
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122 Jiangsu, People's Republic of China.
authors: Peipei
authors: P
authors: Yu
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000378:metabolism; D004553:Electric Conductivity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000166:cytology / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000166:cytology / Q000187:drug effects*; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011753:Pyrones / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009844:Oligosaccharides; D011753:Pyrones; C011890:kojic acid; C005444:N-phenyl-1-naphthylamine; D048271:Chitosan; D015057:1-Naphthylamine; D005954:Glucosephosphate Dehydrogenase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase
keywords: Antibacterial activity; Chitosan oligosaccharides; Kojic acid; Membrane integrity; Membrane permeability