title: Comparative proteomic analysis of Bactrocera dorsalis (Hendel) in response to thermal stress.
abstract: Temperature is one of the most important environmental variables affecting growth, reproduction and distribution of insects. The rise of comparative proteomics provides a powerful tool to explore the response in proteins to thermal stress. As an important worldwide pest, the oriental fruit fly Bactrocera dorsalis causes severe economic losses to crops. To understand the response of B. dorsalis to thermal stress, we performed a comparative proteome analysis of this insect after exposure to extreme low and high temperatures using two-dimensional electrophoresis. Among the separated proteins, 51 diverse protein spots were present differently in response to extreme temperatures. Using tandem mass spectrometry sequencing analysis 39 proteins were successfully identified, which included 13 oxidoreductases, 10 binding proteins, 5 transferases, and 2 each of lyases, isomerases, ligases, and developmental proteins. Subsequently, the expression of these protein transcripts was studied by RT-qPCR to validate the proteomic results. In conclusion, this study provides a first look into the thermal stress response of B. dorsalis at the protein level, and thus it paves the way for further functional studies in the physiological mechanism related to thermal stress.
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Dong
authors: D
authors: Wei
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Fu-Xian
authors: FX
authors: Jia
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Chuan-Bei
authors: CB
authors: Tian
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Yi
authors: Y
authors: Tian
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; Department of Crop Protection, Ghent University, B-9000 Ghent, Belgium.
authors: Guy
authors: G
authors: Smagghe
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
authors: Wei
authors: W
authors: Dou
authors: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China. Electronic address: jjwang7008@yahoo.com.
authors: Jin-Jun
authors: JJ
authors: Wang
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D013696:Temperature; D033621:Tephritidae / Q000737:chemistry / Q000502:physiology*
chemical_list: D019476:Insect Proteins; D020543:Proteome
keywords: Bactrocera dorsalis; Mass spectrometry; Proteomics; Thermal stress; Two-dimensional gel electrophoresis