title: A magnetic nanoparticle-based time-resolved fluoroimmunoassay for determination of the cytokeratin 19 fragment in human serum.
abstract: A sensitive, rapid and novel measurement method for cytokeratin 19 fragment (CYFRA 21-1) in human serum by magnetic particle-based time-resolved fluoroimmunoassay (TRFIA) is described. Built on a sandwich-type immunoassay format, analytes in samples were captured by one monoclonal antibody coating onto the surface of magnetic beads and "sandwiched" by another monoclonal antibody labeled with europium chelates. The coefficient variations of the method were lower than 7%, and the recoveries were in the range of 90-110% for serum samples. The lower limit of quantitation of the present method for CYFRA 21-1 was 0.78 ng/ml. The correlation coefficient of CYFRA 21-1 values obtained by our novel TRFIA and CLIA was 0.980. The present novel TRFIA demonstrated high sensitivity, wider effective detection range and excellent reproducibility for determination of CYFRA 21-1 can be useful for early screening and prognosis evaluation of patients with non-small cell lung cancer.
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Guanfeng
authors: G
authors: Lin
authors: Tiancai
authors: T
authors: Liu
authors: Jingyuan
authors: J
authors: Hou
authors: Zhiqi
authors: Z
authors: Ren
authors: Jianwei
authors: J
authors: Zhou
authors: Qianni
authors: Q
authors: Liang
authors: Zhenhua
authors: Z
authors: Chen
authors: Wenqi
authors: W
authors: Dong
authors: Yingsong
authors: Y
authors: Wu
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D002138:Calibration; D005240:Feasibility Studies; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D053539:Keratin-19 / Q000097:blood*; D057230:Limit of Detection; D016014:Linear Models; D059346:Magnets / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D053539:Keratin-19; C102012:antigen CYFRA21.1