title: Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate.
abstract: Boron compounds have an ability of supporting antioxidant properties in human and animal tissues. Lithium metaborate dihydrate (LiBO2·2H2O; LMD) is commonly used in nonlinear optic materials, cellular phones and pagers. But, there are limited data on the genotoxic and antioxidant effects of LMD in cultured human whole blood cells. The aim of this study was to evaluate for the genotoxicity and antioxidant/oxidant activity of LMD on human whole blood lymphocytes (n = 5). LMD was applied at various concentrations (0-1,280 µg/ml) to cultured blood samples. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity levels. Micronuclei and chromosomal aberration tests were used in genotoxicity studies. Our results clearly revealed that all tested concentrations of LMD were found to be non-genotoxic when compared to that of the control group. In addition, LMD exhibited antioxidant activities at low concentrations. In addition the TOS levels were not changed at all concentrations of LMD. Consequently, our results clearly demonstrated that LMD is non-genotoxic and it has an important antioxidant potential in vitro.
authors: Department of Chemistry, Science and Letter Faculty, Bitlis Eren University, 1300, Bitlis, Turkey. celikezen@hotmail.com.
authors: Fatih Çağlar
authors: FÇ
authors: Çelikezen
authors: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
authors: Başak
authors: B
authors: Toğar
authors: Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
authors: Fatma Betül
authors: FB
authors: Özgeriş
authors: Department of Chemistry, Science and Letter Faculty, Bitlis Eren University, 1300, Bitlis, Turkey.
authors: Mehmet Sait
authors: MS
authors: İzgi
authors: Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
authors: Hasan
authors: H
authors: Türkez
keywords: Chromosomal aberration; Lithium metaborate dihydrate; Micronuclei; Total antioxidant capacity; Total oxidant status