title: Detection of a new cluster of porcine circovirus type 2b strains in domestic pigs in Germany.
abstract: PCV2 can be divided into three different genotypes: PCV2a, PCV2b and PCV2c. Since 2004/2005 PCV2b has become the predominant genotype in the domestic pig population worldwide. In the years 2010 and 2012 PCV2b mutant strains (mPCV2), classified as PCV2b-1C strains, were detected in porcine circovirus diseases (PCVD) affected pigs in China and the United States, respectively. Within one year (April 2013-April 2014) newly emerging mPCV2 strains were isolated in seven German pig farms routinely vaccinating against PCV2. Histopathological, clinical and molecular biological findings including in-situ hybridization (ISH) and real-time PCR indicate PCVD in the affected animals. Characterized isolates from five farms were closely related to the PCV2b-1C reference strain BDH (GenBank no. HM038017), whereas strains from two other farms were only 99.1% and 99.0% identical (based on the nucleotide sequence of the complete genome) to mPCV2 strain BDH, respectively.
authors: Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, Sonnenstrasse 16, 85764 Oberschleissheim, Germany. Electronic address: m.eddicks@lmu.de.
authors: M
authors: M
authors: Eddicks
authors: Institute for Infectious Diseases and Zoonosis, Ludwig-Maximilians University, 80539 Munich, Germany.
authors: R
authors: R
authors: Fux
authors: Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, Sonnenstrasse 16, 85764 Oberschleissheim, Germany.
authors: F
authors: F
authors: Szikora
authors: Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, 80539 Munich, Germany.
authors: L
authors: L
authors: Eddicks
authors: Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, 80539 Munich, Germany.
authors: M
authors: M
authors: Majzoub-Altweck
authors: Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, 80539 Munich, Germany.
authors: W
authors: W
authors: Hermanns
authors: Institute for Infectious Diseases and Zoonosis, Ludwig-Maximilians University, 80539 Munich, Germany.
authors: G
authors: G
authors: Sutter
authors: Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, Sonnenstrasse 16, 85764 Oberschleissheim, Germany.
authors: A
authors: A
authors: Palzer
authors: Zoetis GmbH, Schellingstraße 1, 10785 Berlin, Germany.
authors: E
authors: E
authors: Banholzer
authors: Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, Sonnenstrasse 16, 85764 Oberschleissheim, Germany.
authors: M
authors: M
authors: Ritzmann
mesh_terms: D000818:Animals; D001483:Base Sequence; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics*; D021821:Communicable Diseases, Emerging / Q000662:veterinary* / Q000821:virology; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D017403:In Situ Hybridization / Q000662:veterinary; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*
keywords: PCV2 mutant; PCV2b-1C; PCVD; Vaccination failure