title: Genomic characterization of novel circular ssDNA viruses from insectivorous bats in Southern Brazil.
abstract: Circoviruses are highly prevalent porcine and avian pathogens. In recent years, novel circular ssDNA genomes have recently been detected in a variety of fecal and environmental samples using deep sequencing approaches. In this study the identification of genomes of novel circoviruses and cycloviruses in feces of insectivorous bats is reported. Pan-reactive primers were used targeting the conserved rep region of circoviruses and cycloviruses to screen DNA bat fecal samples. Using this approach, partial rep sequences were detected which formed five phylogenetic groups distributed among the Circovirus and the recently proposed Cyclovirus genera of the Circoviridae. Further analysis using inverse PCR and Sanger sequencing led to the characterization of four new putative members of the family Circoviridae with genome size ranging from 1,608 to 1,790 nt, two inversely arranged ORFs, and canonical nonamer sequences atop a stem loop.
authors: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul (RS), Brazil.
authors: Francisco Esmaile de Sales
authors: FE
authors: Lima
authors: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul (RS), Brazil; FEPAGRO Animal Health, Institute of Veterinary Research "Desidério Finamor" (IPVDF), Eldorado do Sul, Rio Grande do Sul (RS), Brazil.
authors: Samuel Paulo
authors: SP
authors: Cibulski
authors: FEPAGRO Animal Health, Institute of Veterinary Research "Desidério Finamor" (IPVDF), Eldorado do Sul, Rio Grande do Sul (RS), Brazil.
authors: Helton Fernandes
authors: HF
authors: Dos Santos
authors: FEPAGRO Animal Health, Institute of Veterinary Research "Desidério Finamor" (IPVDF), Eldorado do Sul, Rio Grande do Sul (RS), Brazil.
authors: Thais Fumaco
authors: TF
authors: Teixeira
authors: FEPAGRO Animal Health, Institute of Veterinary Research "Desidério Finamor" (IPVDF), Eldorado do Sul, Rio Grande do Sul (RS), Brazil.
authors: Ana Paula Muterle
authors: AP
authors: Varela
authors: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul (RS), Brazil; FEPAGRO Animal Health, Institute of Veterinary Research "Desidério Finamor" (IPVDF), Eldorado do Sul, Rio Grande do Sul (RS), Brazil.
authors: Paulo Michel
authors: PM
authors: Roehe
authors: Blood Systems Research Institute, San Francisco, California, United States of America; University of California, Department of Laboratory Medicine, San Francisco, California, United States of America.
authors: Eric
authors: E
authors: Delwart
authors: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul (RS), Brazil.
authors: Ana Cláudia
authors: AC
authors: Franco
mesh_terms: D000818:Animals; D001938:Brazil; D002685:Chiroptera / Q000502:physiology / Q000821:virology*; D018136:Circoviridae / Q000145:classification / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004435:Eating; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D023281:Genomics; D007313:Insecta; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D004277:DNA, Single-Stranded