title: The antinociceptive effect of stimulating the retrosplenial cortex in the rat tail-flick test but not in the formalin test involves the rostral anterior cingulate cortex.
abstract: The stimulation of the retrosplenial cortex (RSC) is antinociceptive in the rat tail-flick and formalin tests. The rat RSC is caudal to and send projections to the ipsilateral anterior cingulate cortex (ACC), which is also involved in pain processing. This study demonstrated that pre-treating the rostral (rACC), but not the caudal ACC with CoCl2 (1mM), or the rACC ablation increased the duration of the antinociceptive effect evoked by a 15-s period of electrical stimulation (AC, 60Hz, 20μA) of the RSC in the rat tail-flick. Injecting the GABA-A antagonist bicuculline (50ng/0.25μL), but not the GABA-B antagonist phaclofen (300ng/0.25μL) into the rACC also increased the duration of the stimulation-induced antinociception from the RSC. In contrast, the effects of rACC stimulation persisted after the injection of CoCl2 (1mM) into the RSC. The injection of CoCl2 into the rACC did not change the nociceptive behavior of rats during phase 1 of the formalin response but reduced licking response duration during phase 2. This effect was similar in sham or stimulated animals at the RSC. We conclude that the antinociceptive effect of stimulating the RSC in the rat tail-flick test is modulated by the rACC involving GABA-A receptors in this cortex. In contrast, the antinociceptive effect of stimulating the RSC in the formalin test does not involve the rACC.
authors: Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, SP 14049-900, Brazil.
authors: Gláucia Melo
authors: GM
authors: Reis
authors: Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, SP 14049-900, Brazil.
authors: Rafael Sobrano
authors: RS
authors: Fais
authors: Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, SP 14049-900, Brazil. Electronic address: wadprado@fmrp.usp.br.
authors: Wiliam A
authors: WA
authors: Prado
mesh_terms: D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D002540:Cerebral Cortex / Q000502:physiology*; D003035:Cobalt / Q000494:pharmacology; D004558:Electric Stimulation; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology; D006179:Gyrus Cinguli / Q000502:physiology*; D008297:Male; D010147:Pain Measurement; D058748:Pain Perception / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013623:Tail
chemical_list: D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; C052329:phaclofen; D003035:Cobalt; C018021:cobaltous chloride; D001418:Baclofen; D001640:Bicuculline
keywords: Anterior cingulate cortex; Antinociception; Retrosplenial cortex; Tail-flick test