title: Identification of the microbial community composition and structure of coal-mine wastewater treatment plants.
abstract: The wastewater from coal-mine industry varies greatly and is resistant to biodegradation for containing large quantities of inorganic and organic pollutants. Microorganisms in activated sludge are responsible for the pollutants' removal, whereas the microbial community composition and structure are far from understood. In the present study, the sludges from five coal-mine wastewater treatment plants were collected and the microbial communities were analyzed by Illumina high-throughput sequencing. The diversities of these sludges were lower than that of the municipal wastewater treatment systems. The most abundant phylum was Proteobacteria ranging from 63.64% to 96.10%, followed by Bacteroidetes (7.26%), Firmicutes (5.12%), Nitrospira (2.02%), Acidobacteria (1.31%), Actinobacteria (1.30%) and Planctomycetes (0.95%). At genus level, Thiobacillus and Comamonas were the two primary genera in all sludges, other major genera included Azoarcus, Thauera, Pseudomonas, Ohtaekwangia, Nitrosomonas and Nitrospira. Most of these core genera were closely related with aromatic hydrocarbon degradation and denitrification processes. Identification of the microbial communities in coal-mine wastewater treatment plants will be helpful for wastewater management and control.
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Qiao
authors: Q
authors: Ma
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: qyy@dlut.edu.cn.
authors: Yuan-Yuan
authors: YY
authors: Qu
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Xu-Wang
authors: XW
authors: Zhang
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Wen-Li
authors: WL
authors: Shen
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Zi-Yan
authors: ZY
authors: Liu
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Jing-Wei
authors: JW
authors: Wang
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Zhao-Jing
authors: ZJ
authors: Zhang
authors: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors: Ji-Ti
authors: JT
authors: Zhou
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D058448:Biota; D016000:Cluster Analysis; D000066428:Coal Industry; D014644:Genetic Variation; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D007220:Industrial Waste; D009566:Nitrates / Q000378:metabolism; D010802:Phylogeny; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D006841:Hydrocarbons, Aromatic; D007220:Industrial Waste; D009566:Nitrates; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Activated sludge; Coal-mine wastewater; Illumina high-throughput sequencing; Microbial community