title: Anti-inflammatory effects of zinc in PMA-treated human gingival fibroblast cells.
abstract: OBJECTIVE
  Abnormal cellular immune response has been considered to be responsible for oral lesions in recurrent aphthous stomatitis. Zinc has been known to be an essential nutrient metal that is necessary for a broad range of biological activities including antioxidant, immune mediator, and anti-inflammatory drugs in oral mucosal disease. The objective of this study was to investigate the effects of zinc in a phorbol-12-myristate-13-acetate (PMA)-treated inflammatory model on human gingival fibroblast cells (hGFs).
  
  
  METHODS
  Cells were pre-treated with zinc chloride, followed by PMA in hGFs. The effects were assessed on cell viability, cyclooxygenease-1,2(COX-1,2) protein expression, PGE2 release, ROS production and cytokine release, Results: The effects were assessed on cell viability, COX1/2 protein expression, PGE2 release, ROS production, cytokine release. The results showed that, in the presence of PMA, zinc treatment leads to reduce the production of ROS, which results in decrease of COX-2 expression and PGE2 release.
  
  
  CONCLUSIONS
  Thus, we suggest that zinc treatment leads to the mitigation of oral inflammation and may prove to be an alternative treatment for recurrent aphthous stomatitis.
authors: Department of Oral Pathology, Dental Science Research Institute and Medical Research, Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Bug-Gu, Gwangju, 500-757, Korea, js3894@chonnam.ac.kr.
authors: Jisun
authors: J
authors: Kim
authors: Sangwoo
authors: S
authors: Kim
authors: Sangmi
authors: S
authors: Jeon
authors: Zheng
authors: Z
authors: Hui
authors: Young
authors: Y
authors: Kim
authors: Yeonggwan
authors: Y
authors: Im
authors: Wonbong
authors: W
authors: Lim
authors: Changsu
authors: C
authors: Kim
authors: Hongran
authors: H
authors: Choi
authors: Okjoon
authors: O
authors: Kim
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000031:analogs & derivatives; D015032:Zinc / Q000494:pharmacology*
chemical_list: D016207:Cytokines; C016658:phorbolol myristate acetate; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate