title: Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein.
abstract: β-Conglycinin is a major seed storage protein in soybeans, which are an important source of protein. The major subunits (α, α' and β) of β-conglycinin are sorted to protein-storage vacuoles in seed cells. Vacuolar sorting receptor (VSR) is an integral membrane protein that recognizes the sorting determinant of vacuolar proteins, including β-conglycinin, and regulates their sorting process. Vacuolar sorting determinants of the α' and β subunits of β-conglycinin exist in their C-terminal peptides. Here, the preliminary X-ray diffraction analysis of the binding domain of soybean VSR crystallized with the peptide responsible for the sorting determinant in β-conglycinin is reported. X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to space group P3121, with unit-cell parameters a = b = 116.4, c = 86.1 Å.
authors: Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors: Nobuyuki
authors: N
authors: Maruyama
authors: Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors: Tomohiro
authors: T
authors: Goshi
authors: Graduate School of Science, Osaka University, Machikaneyama 1-3, Toyonaka, Osaka 560-8531, Japan.
authors: Shigeru
authors: S
authors: Sugiyama
authors: Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Mayumi
authors: M
authors: Niiyama
authors: SOSHO Inc., 313 Photonics Center Building, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Hiroaki
authors: H
authors: Adachi
authors: SOSHO Inc., 313 Photonics Center Building, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Kazufumi
authors: K
authors: Takano
authors: SOSHO Inc., 313 Photonics Center Building, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Satoshi
authors: S
authors: Murakami
authors: Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Tsuyoshi
authors: T
authors: Inoue
authors: Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Yusuke
authors: Y
authors: Mori
authors: Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
authors: Hiroyoshi
authors: H
authors: Matsumura
authors: Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors: Bunzo
authors: B
authors: Mikami
mesh_terms: D052179:Antigens, Plant / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000737:chemistry*; D055314:Seed Storage Proteins / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D010455:Peptides; D011956:Receptors, Cell Surface; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max
keywords: seed storage protein; soybean; vacuolar sorting receptor; β-conglycinin