title: Identification of medicinal species and antifungal property of a Dong ethnic drug.
abstract: OBJECTIVE
  To identify the medicinal species in the Zi Hua Di Ding (ZHDD) prescription commonly used by the Dong ethnic people, and investigate the potential mechanism involved with the anti-fungal properties on Trichophyton rubrum.
  
  
  METHODS
  DNA barcode technique was used to identify the species in the ZHDD prescription. In vitro study was performed to investigate the antifungal properties of the identified Viola philippica on the T. rubrum. Microscopy was used to observe the growth of the T. rubrum.
  
  
  RESULTS
  The ZHDD prescription is a mixture of V. philippica and V. inconspicua, and its antifungal properties was superior to the single component. V. philippica could affect the surface and flexibility of hypha, and contribute to the generation of branches. In addition, it could damage the biofilm.
  
  
  CONCLUSIONS
  Using the DNA barcode technique, we identify the ZHDD prescription is a mixture of V. philippica and V. inconspicua, and its antifungal properties was superior to the single component. V. philippica could affect the growth and biofilm formation of T. rubrum.
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100102, China.
authors: Zhirong
authors: Z
authors: Sun
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100102, China.
authors: Yuan
authors: Y
authors: Du
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100102, China.
authors: Lili
authors: L
authors: Cheng
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100102, China.
authors: Nannan
authors: N
authors: Zhu
keywords: DNA barcode; Dong ethnic drug; Trichophyton rubrum; Viola philippica; antifungal property