title: Supplementation of apelin increase plasma levels of nesfatin-1 in normal and DOCA-salt hypertensive rats.
abstract: OBJECTIVE
  We aimed to observe the effects of apelin supplementation on the plasma levels of nesfatin-1 in DOCA-salt hypertensive and normal rats.
  
  
  METHODS
  For this purpose, 28 young Wistar albino male rats were divided into four groups; Control (C), Control + Apelin (C+A), Hypertension (HT) and Hypertension + Apelin (HT+A). Hypertension was induced by injection of DOCA-salt (25 mg/kg, s.c.) twice weekly, 4 weeks, whereas intraperitoneal apelin was administered (200 µg.kg-1) for 17 days. Plasma nesfatin-1 and apelin levels were measured with ELISA. Systolic blood pressure was monitored using a tail cuff system. The relationships between plasma nesfatin levels and blood pressure were assessed.
  
  
  RESULTS
  Plasma nesfatin-1 levels was found lower in control animals compared to C+A, HT and HT+A groups (p = 0.002, p = 0.026 and p = 0.011, respectively). Systolic blood pressures were similar in the C and C+A groups, but systolic blood pressures of the HT and HT+A groups was found significantly higher than the C and C+A groups.
  
  
  CONCLUSIONS
  In conclusion, apelin administration induced an increment of nesfatin-1 in normal rats and plasma levels of nesfatin-1 increase in DOCA-salt hypertension rats. But apelin addition in hypertension did not cause an extra increase in nesfatin-1 levels. This is the first report to investigate the effect of apelin administration on plasma nesfatin levels of normal and hypertensive rats (Fig. 2, Ref. 44).
authors: R
authors: R
authors: Akcilar
authors: C
authors: C
authors: Ayada
authors: G
authors: G
authors: Turgut
authors: S
authors: S
authors: Turgut
mesh_terms: D000818:Animals; D000073861:Apelin; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000097:blood* / Q000187:drug effects*; D064791:Desoxycorticosterone Acetate; D004195:Disease Models, Animal; D006973:Hypertension / Q000097:blood* / Q000139:chemically induced / Q000188:drug therapy*; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000097:blood* / Q000187:drug effects*; D000080866:Nucleobindins; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000073861:Apelin; C498579:Apln protein, rat; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D064791:Desoxycorticosterone Acetate