title: [Effect of has-miR-150 on proliferation and apoptosis of Jurkat cells and its mechanism].
abstract: OBJECTIVE
  This study was to investigate the effect of has-miR-150 on the proliferation and apoptosis in human acute T lymphocytic leukemia (T-ALL) cell line Jurkat, and explore its mechanism.
  
  
  METHODS
  Lentivirus-has-miR-150 was constructed and transfected to Jurkat cells. The expression of miR-150 was detected by real time PCR; the cell proliferation and apoptosis were detected by CCK-8 method and Annexin V/7-AAD labeling, respectively; the cell-related protein expressions of phosphatidylinositol-3-kinase(PI3K)/serine/ threonine kinase (Akt) signaling pathway were detected by Western blot.
  
  
  RESULTS
  The expression of miR-150 in infected Jurkat cells was significantly upregulated by constructing lentivirus-has-miR-150. Compared to negative control (transfected with empty-vector lentivirus), the cell proliferation after LV-miR-150 transfection was significantly inhibited and cell apoptosis was remarkably induced. Phosphorylation levels of P13K/Akt/NF-κB signaling pathway protein p-Akt and p-p65 decreased,whereas no obvious change was found in the expression of Akt.
  
  
  CONCLUSIONS
  miR-150 may be a putative oncoprotein in T-ALL cells. Overexpression of miR-150 has noticeable effects on the proliferation inhibition and apoptosis induction of Jurkat cells, which may be mediated by the negative regulation of PI3K/Akt /NF-κB signaling pathway.
authors: Department of Hematology,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province, China.
authors: Ying
authors: Y
authors: Wang
authors: Department of Hematology,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province, China.
authors: Wei
authors: W
authors: Sang
authors: Department of Hematology,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province, China.
authors: Cai
authors: C
authors: Sun
authors: Department of Hematology,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province, China.
authors: Ling-Yu
authors: LY
authors: Zeng
authors: Department of Hematology,The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province, China. E-mail: linhmd@163.com.
authors: Kai-Lin
authors: KL
authors: Xu
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D006801:Humans; D019169:Jurkat Cells; D016086:Lentivirus; D035683:MicroRNAs; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases