title: Simultaneous Detection of Disseminated and Circulating Tumor Cells in Primary Breast Cancer Patients.
abstract: OBJECTIVE
  Disseminated tumor cells (DTCs) from bone marrow (BM) are a surrogate of minimal residual disease (MRD) in primary breast cancer (PBC) patients and associated with an adverse prognosis. However, BM sampling is an invasive procedure. Although there is growing evidence that circulating tumor cells (CTCs) from the blood are also suitable for monitoring MRD, data on the simultaneous detection of DTCs and CTCs are limited.
  
  
  METHODS
  We determined the presence of DTCs using immunocytochemistry and the pan-cytokeratin antibody A45-B/B3. CTCs were determined simultaneously using a reverse transcription-polymerase chain reaction-based assay (AdnaTest Breast Cancer) and CellSearch (at least one CTC per 7.5 mL blood). We compared the detection of DTCs and CTCs and evaluated their impact on disease-free and overall survival.
  
  
  RESULTS
  Of 585 patients, 131 (22%) were positive for DTCs; 19 of 202 (9%) and 18 of 383 (5%) patients were positive for CTCs, as shown by AdnaTest and CellSearch, respectively. No significant association was observed between DTCs and CTCs (p=0.248 and p=0.146 as shown by AdnaTest and CellSearch, respectively). The presence of DTCs (p=0.046) and the presence of CTCs as shown by CellSearch (p=0.007) were predictive of disease-free survival.
  
  
  CONCLUSIONS
  Our data confirm the prognostic relevance of DTCs and CTCs in patients with PBC. As we found no significant relationship between DTCs and CTCs, prospective trials should include their simultaneous detection. Within those trials, the question of whether or not DTCs and CTCs are independent subpopulations of malignant cell clones should be determined by molecular characterization.
authors: Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany.
authors: Andreas D
authors: AD
authors: Hartkopf
authors: Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg, Germany.
authors: Markus
authors: M
authors: Wallwiener
authors: Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany.
authors: Markus
authors: M
authors: Hahn
authors: Department of Obstetrics and Gynecology, University of Duesseldorf, Duesseldorf, Germany.
authors: Tanja N
authors: TN
authors: Fehm
authors: Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany.
authors: Sara Y
authors: SY
authors: Brucker
authors: Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany.
authors: Florin-Andrei
authors: FA
authors: Taran
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis / Q000175:diagnosis; D009360:Neoplastic Cells, Circulating; D011379:Prognosis; D011446:Prospective Studies
keywords: Bone marrow; Breast neoplasms; Circulating neoplastic cells; Prognosis; Residual neoplasm