title: Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration.
abstract: Cricket nymphs have the remarkable ability to regenerate a functional leg following amputation, indicating that the regenerating blastemal cells contain information for leg morphology. However, the molecular mechanisms that underlie regeneration of leg patterns remain poorly understood. Here, we analyzed phenotypes of the tibia and tarsus (three tarsomeres) obtained by knockdown with regeneration-dependent RNA interference (rdRNAi) against Gryllus dachshund (Gb'dac) and Distal-less (Gb'Dll). We found that depletion of Gb'Dll mRNA results in loss of the tarsal segments, while rdRNAi against Gb'dac shortens the tibia at the two most distal tarsomeres. These results indicate that Gb'Dll expression is indispensable for formation of the tarsus, while Gb'dac expression is necessary for elongation of the tibia and formation of the most proximal tarsomere. These findings demonstrate that mutual transcriptional regulation between the two is indispensable for formation of the tarsomeres, whereas Gb'dac is involved in determination of tibial size through interaction with Gb'ds/Gb'ft.
authors: Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima City, Tokushima, 770-8506, Japan.
authors: Yoshiyasu
authors: Y
authors: Ishimaru
authors: Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima City, Tokushima, 770-8506, Japan.
authors: Taro
authors: T
authors: Nakamura
authors: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama City, Okayama, 700-8530, Japan.
authors: Tetsuya
authors: T
authors: Bando
authors: Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima City, Tokushima, 770-8506, Japan.
authors: Yuji
authors: Y
authors: Matsuoka
authors: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama City, Okayama, 700-8530, Japan.
authors: Hideyo
authors: H
authors: Ohuchi
authors: Center for Collaboration among Agriculture, Industry and Commerce, The University of Tokushima, 2-24 Shinkura-cho, Tokushima City, Tokushima 770-8501, Japan.
authors: Sumihare
authors: S
authors: Noji
authors: Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima City, Tokushima, 770-8506, Japan.
authors: Taro
authors: T
authors: Mito
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D049109:Cell Proliferation; D005121:Extremities; D005786:Gene Expression Regulation; D017344:Genes, Insect; D006135:Gryllidae / Q000502:physiology*; D008954:Models, Biological; D010641:Phenotype; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics; D014158:Transcription, Genetic