title: Applicability evaluation of Deep Eutectic Solvents-Cellulase system for lignocellulose hydrolysis.
abstract: Deep Eutectic Solvents (DESs) have recently emerged as a new generation of ionic liquids for lignocellulose pretreatment. However, DESs contain salt components which tend to inactivate cellulase in the subsequent saccharification process. To alleviate this problem, it is necessary to evaluate the applicability of the DESs-Cellulase system. This was accomplished in the present study by first studying the stability of cellulase in the presence of selected DESs followed by applicability evaluation based on glucose production, energy consumption and kinetic performance. Results showed that the cellulase was able to retain more than 90% of its original activity in the presence of 10% (v/v) for glycerol based DES (GLY) and ethylene glycol based DES (EG). Furthermore, both DESs system exhibited higher glucose percentage enhancement and lower energy consumption as compared to diluted alkali system. Among the two DESs studied, EG showed comparatively better kinetic performance.
authors: School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
authors: Ahmad Anas Nagoor
authors: AA
authors: Gunny
authors: School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia. Electronic address: dachyar@unimap.edu.my.
authors: Dachyar
authors: D
authors: Arbain
authors: Chemical and Environmental Engineering (CEE), Masdar Institute for Science and Technology, PO Box 54224, Abu Dhabi, United Arab Emirates.
authors: Enas Muen
authors: EM
authors: Nashef
authors: Bioenvironmental Engineering Research Center (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, 50728 Gombak, Kuala Lumpur, Malaysia.
authors: Parveen
authors: P
authors: Jamal
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D055162:Biocatalysis / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry; D004795:Enzyme Stability / Q000187:drug effects; D019855:Ethylene Glycol / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000494:pharmacology; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry; D012972:Sodium Hydroxide / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry*; D014866:Waste Products
chemical_list: D012997:Solvents; D014866:Waste Products; C036909:lignocellulose; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; D019855:Ethylene Glycol; D005947:Glucose; C109691:microcrystalline cellulose; D005990:Glycerol
keywords: Cellulase; Deep Eutectic Solvents; Lignocellulose; Saccharification kinetics