title: Continuous alkaline pretreatment of Miscanthus sacchariflorus using a bench-scale single screw reactor.
abstract: Miscanthus sacchariflorus 'Goedae-Uksae 1' (GU) was developed as an energy crop of high productivity in Korea. For the practical use of GU for bioethanol production, a bench-scale continuous pretreatment system was developed. The reactor performed screw extrusion, soaking and thermochemical pretreatment at the following operating conditions: 3 mm particle size, 22% moisture content, 140 °C reaction temperature, 8 min residence time, 15 g/min biomass feeding and 120 mL/min NaOH input. As a result of minimizing NaOH concentration and enzyme dosage, 90.8±0.49% glucose yield was obtained from 0.5 M NaOH-pretreated GU containing 3% glucan with 10 FPU cellulase/g cellulose at 50 °C for 72 h. The separate hydrolysis and fermentation of 0.5 M NaOH-pretreated GU containing 10% glucan with 10-30 FPU for 102 h produced 43.0-49.6 g/L bioethanol (theoretical yield, 75.8-87.6%). Thus, this study demonstrated that continuous pretreatment using a single screw reactor is effective for bioethanol production from Miscanthus biomass.
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Young-Lok
authors: YL
authors: Cha
authors: School of Life Sciences and Biotechnology for BK21 PLUS, Korea University, Seoul 136-713, Republic of Korea. Electronic address: dbl3jwy@korea.ac.kr.
authors: Jungwoo
authors: J
authors: Yang
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Yuri
authors: Y
authors: Park
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Gi Hong
authors: GH
authors: An
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Jong-Woong
authors: JW
authors: Ahn
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Youn-Ho
authors: YH
authors: Moon
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Young-Mi
authors: YM
authors: Yoon
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: Gyeong-Dan
authors: GD
authors: Yu
authors: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea.
authors: In-Hu
authors: IH
authors: Choi
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D006109:Poaceae / Q000187:drug effects*; D012972:Sodium Hydroxide / Q000494:pharmacology*
chemical_list: D012972:Sodium Hydroxide; D002480:Cellulase
keywords: Alkaline pretreatment; Bioethanol; Continuous pretreatment; Lignocellulosic biomass; Miscanthus