title: Diabetes detrimental effects on enamel and dentine formation.
abstract: OBJECTIVE
  Understanding morphological changes and mineral content of tooth hard tissues may influence dental treatment. In this study, the effect of Type 1 Diabetes Mellitus (T1DM) on tooth structure was examined.
  
  
  METHODS
  Experimental T1DM was induced in 3-week old male Wistar rats (n=10) by a single dose of 60mg/kg body weight of Streprozotocin. All rats were injected with calcein twice during the experiment and sacrificed at the age of 7 weeks old. Micro-computed tomography (micro-CT) was used to determine the mineral density and thickness of enamel and dentine. Also, a histomorphometery study was conducted to detect the rates of dentine mineral apposition and formation. The examined area was in the crown analogue of the rat mandibular incisor parallel to the long axis of the mesial surface of the first molar. All results were compared using Students' t-test (p<0.05).
  
  
  RESULTS
  Results showed that the enamel and dentine thickness were significantly reduced (hypoplasia) and there was a significant reduction of the rate of dentine mineral apposition and formation, while there was no significant effect of the T1DM condition on the mineral density of enamel and dentine.
  
  
  CONCLUSIONS
  T1DM has a detrimental influence on the formation of enamel and dentine in the early growth stage.
  
  
  CONCLUSIONS
  T1DM condition may alter treatment planning of orthodontic treatment as it is associated with decreased enamel and dentin thickness that may affect teeth size and their resistance to caries.
authors: Orthodontic Science Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudia Arabia; Orthodontic Science, Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence Program, International Research Center For Molecular Science in Tooth and Bone Disease at Tokyo Medical and Dental University, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Alexandria University, Alexandria, Egypt.
authors: M A
authors: MA
authors: Abbassy
authors: Orthodontic Science, Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors: I
authors: I
authors: Watari
authors: Operative Dentistry Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia; Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Egypt. Electronic address: drahmedbakry@gmail.com.
authors: A S
authors: AS
authors: Bakry
authors: Cariology and Operative Dentistry Department, Graduate School of Medical and Dental Sciences, Tokyo Medical & Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Tokyo, Japan.
authors: H
authors: H
authors: Hamba
authors: Orthodontic Science Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudia Arabia.
authors: Ali H
authors: AH
authors: Hassan
authors: Cariology and Operative Dentistry Department, Graduate School of Medical and Dental Sciences, Tokyo Medical & Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Tokyo, Japan.
authors: J
authors: J
authors: Tagami
authors: Orthodontic Science, Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors: T
authors: T
authors: Ono
mesh_terms: D000818:Animals; D003743:Dental Enamel / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D003804:Dentin / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism* / Q000473:pathology; D005452:Fluoresceins / Q000494:pharmacology; D007180:Incisor / Q000187:drug effects / Q000254:growth & development; D008297:Male; D008334:Mandible; D008963:Molar / Q000187:drug effects / Q000254:growth & development; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014074:Tooth Calcification / Q000187:drug effects / Q000502:physiology; D019228:Tooth Crown; D014092:Tooth Root / Q000033:anatomy & histology; D055114:X-Ray Microtomography
chemical_list: D005452:Fluoresceins; C007740:fluorexon
keywords: Dentine thickness; Enamel thickness; Histomorphometry; Micro CT; Tooth mineral density; Type 1 Diabetes Mellitus