title: High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column.
abstract: A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using Chromolith® performance RP-18e (100-4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38-104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography-tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.
authors: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors: Wejdan Shakir
authors: WS
authors: Khayoon
authors: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia. Electronic address: bahrud@usm.my.
authors: Bahruddin
authors: B
authors: Saad
authors: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors: Tien Ping
authors: TP
authors: Lee
authors: School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors: Baharuddin
authors: B
authors: Salleh
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000737:chemistry*; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D012822:Silicon Dioxide
chemical_list: D000348:Aflatoxins; D012822:Silicon Dioxide; D016604:Aflatoxin B1
keywords: Aflatoxins; Foodstuffs; High performance liquid chromatography; Liquid chromatography–tandem mass spectrometry; Monolithic column