title: Live-cell MRI with xenon hyper-CEST biosensors targeted to metabolically labeled cell-surface glycans.
abstract: The targeting of metabolically labeled glycans with conventional MRI contrast agents has proved elusive. In this work, which further expands the utility of xenon Hyper-CEST biosensors in cell experiments, we present the first successful molecular imaging of such glycans using MRI. Xenon Hyper-CEST biosensors are a novel class of MRI contrast agents with very high sensitivity. We designed a multimodal biosensor for both fluorescent and xenon MRI detection that is targeted to metabolically labeled sialic acid through bioorthogonal chemistry. Through the use of a state of the art live-cell bioreactor, it was demonstrated that xenon MRI biosensors can be used to image cell-surface glycans at nanomolar concentrations.
authors: ERC Project Biosensor Imaging, Leibniz-Institut für Molekulare Pharmakologie, Berlin (Germany).
authors: Christopher
authors: C
authors: Witte
authors: Vera
authors: V
authors: Martos
authors: Honor May
authors: HM
authors: Rose
authors: Stefan
authors: S
authors: Reinke
authors: Stefan
authors: S
authors: Klippel
authors: Leif
authors: L
authors: Schröder
authors: Christian P R
authors: CP
authors: Hackenberger
mesh_terms: D015374:Biosensing Techniques; D002470:Cell Survival; D003287:Contrast Media / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D057054:Molecular Imaging; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D014978:Xenon / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D011134:Polysaccharides; D014978:Xenon; D019158:N-Acetylneuraminic Acid
keywords: bioorthogonal labeling; biosensors; glycosylation; host-guest systems; xenon