title: Callicarpa japonica Thunb. reduces inflammatory responses: a mouse model of lipopolysaccharide-induced acute lung injury.
abstract: Callicarpa japonica Thunb. (CJT) is traditionally used as an herbal remedy for the treatment of inflammatory diseases. This study aimed to investigate the anti-inflammatory response of CJT in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and LPS-induced acute lung injury (ALI) in mice. The C57BL/6 mice were administered 30 mg/kg of CJT by oral gavage for 3 days. LPS is applied to animals by intranasal administration 1 h after final CJT treatment. LPS is applied to animals by intranasal administration 1h after final CJT treatment. LPS was delivered intranasally 1h after the final CJT treatment. In the LPS-stimulated RAW264.7 cells, CJT significantly decreased nitric oxide (NO) and interleukin (IL)-6 in a concentration-dependent manner by reducing inducible NO synthase (iNOS) and IL-6 mRNA levels. In the ALI model, CJT decreased the inflammatory cell count in the bronchoalveolar lavage fluid (BALF) while IL-6 levels were reduced in CJT-treated mice compared with the ALI control mice. CJT also inhibited airway inflammation by reducing iNOS expression in lung tissue. In conclusion, our results indicate that CJT inhibits inflammatory responses in LPS-stimulated RAW264.7 cells and in the LPS-induced ALI model. Therefore, we suggest that CJT has the potential to treat inflammatory diseases such as pneumonia.
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea; Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors: Na-Rae
authors: NR
authors: Shin
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea; College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors: In-Sik
authors: IS
authors: Shin
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Hyuk-Hwan
authors: HH
authors: Song
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Ju-Mi
authors: JM
authors: Hong
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Ok-Kyoung
authors: OK
authors: Kwon
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Chan-Mi
authors: CM
authors: Jeon
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Jung-Hee
authors: JH
authors: Kim
authors: International Biological Material Research Center, Korea Research Institute of Bioscience and Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Sang-Woo
authors: SW
authors: Lee
authors: International Biological Material Research Center, Korea Research Institute of Bioscience and Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Joong-Ku
authors: JK
authors: Lee
authors: Medicinal Plant Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Yunnan, China.
authors: Hang
authors: H
authors: Jin
authors: Medicinal Plant Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Yunnan, China.
authors: Wan Yi
authors: WY
authors: Li
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea.
authors: Sei-Ryang
authors: SR
authors: Oh
authors: Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea. Electronic address: hahnkw@hnu.kr.
authors: Kyu-Woung
authors: KW
authors: Hahn
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: ksahn@kribb.re.kr.
authors: Kyung-Seop
authors: KS
authors: Ahn
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D019686:Lamiaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008810:Mice, Inbred C57BL
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides
keywords: Acute lung injury; Callicarpa japonica Thunb.; Inducible nitric oxide synthase; Interleukin-6