title: Luzp4 defines a new mRNA export pathway in cancer cells.
abstract: Cancer testis antigens (CTAs) represented a poorly characterized group of proteins whose expression is normally restricted to testis but are frequently up-regulated in cancer cells. Here we show that one CTA, Luzp4, is an mRNA export adaptor. It associates with the TREX mRNA export complex subunit Uap56 and harbours a Uap56 binding motif, conserved in other mRNA export adaptors. Luzp4 binds the principal mRNA export receptor Nxf1, enhances its RNA binding activity and complements Alyref knockdown in vivo. Whilst Luzp4 is up-regulated in a range of tumours, it appears preferentially expressed in melanoma cells where it is required for growth.
authors: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK.
authors: Nicolas
authors: N
authors: Viphakone
authors: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK Academic Urology Unit, The University of Sheffield, Beech Hill Road, Sheffield, UK.
authors: Marcus G
authors: MG
authors: Cumberbatch
authors: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK.
authors: Michaela J
authors: MJ
authors: Livingstone
authors: Sheffield Institute for Translational Neuroscience, The University of Sheffield, 385a Glossop Road, Sheffield, UK.
authors: Paul R
authors: PR
authors: Heath
authors: Department of Chemical and Biological Engineering, The University of Sheffield, Mappin Street, Sheffield, UK.
authors: Mark J
authors: MJ
authors: Dickman
authors: Academic Urology Unit, The University of Sheffield, Beech Hill Road, Sheffield, UK.
authors: James W
authors: JW
authors: Catto
authors: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK stuart.wilson@sheffield.ac.uk.
authors: Stuart A
authors: SA
authors: Wilson
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors; D029543:Nucleocytoplasmic Transport Proteins / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014157:Transcription Factors / Q000037:antagonists & inhibitors
chemical_list: C105242:ALYREF protein, human; D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; C456621:LUZP4 protein, human; C122111:NXF1 protein, human; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors