title: Genetic diversity of fusion gene (ORF 117), an analogue of vaccinia virus A27L gene of capripox virus isolates.
abstract: The fusion gene (ORF 117) sequences of twelve (n = 12) capripox virus isolates namely sheeppox (SPPV) and goatpox (GTPV) viruses from India were demonstrated for their genetic and phylogenetic relationship among them. All the isolates were confirmed for their identity by routine PCR before targeting ORF 117 gene for sequence analysis. The designed primers specifically amplified ORF 117 gene as 447 bp fragment from total genomic DNA extracted from all the isolates. Sequence analysis revealed a significant percentage of identity among GTPV, SPPV and between them at both nucleotide and amino acid levels. The topology of the phylogenetic tree revealed that three distinct clusters corresponding to SPPV, GTPV and lumpy skin disease virus was formed. However, SPPV Pune/08 and SPPV Roumanian Fanar isolates were clustered into GTPV group as these two isolates showed a 100 and 99.3 % identity with GTPV isolates of India at nt and aa levels, respectively. Protein secondary structure and 3D view was predicted and found that it has high antigenic index and surface probability with low hydrophobicity, and it can be targeted for expression and its evaluation to explore its diagnostic potential in epidemiological investigation in future.
authors: Division of Virology, Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar, 263 138, Uttarakhand, India.
authors: M
authors: M
authors: Dashprakash
authors: Gnanavel
authors: G
authors: Venkatesan
authors: Muthannan Andavar
authors: MA
authors: Ramakrishnan
authors: Dhanavelu
authors: D
authors: Muthuchelvan
authors: Muthu
authors: M
authors: Sankar
authors: Awadh Bihari
authors: AB
authors: Pandey
authors: Bimelendu
authors: B
authors: Mondal
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D014644:Genetic Variation; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D011213:Poxviridae Infections / Q000662:veterinary* / Q000821:virology; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D014616:Vaccinia virus / Q000737:chemistry / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014760:Viral Fusion Proteins