title: Smad nuclear interacting protein, SNIP1, mediates the ecdysteroid signal transduction in red crayfish Procambarus clarkii.
abstract: A smad nuclear interacting protein 1 (SNIP1) homolog was identified in the crayfish Procambarus clarkii and this gene encoded a polypeptide of 288 amino acids. Phylogenic analysis showed that P. clarkii SNIP1 was highly homologous to that of invertebrates and shared a highly conserved FHA domain of SNIP proteins at the C-terminus. Quantitative real-time PCR (qRT-PCR) analysis showed that Pc-SNIP1 expression was higher in heart than that in other examined tissues. In addition, prokaryotic expression and purification of the recombinant SNIP1 protein were performed. SDS-PAGE and western blot analysis demonstrated that a 35 KDa recombinant protein was successfully expressed in Escherichia coli cells. The expression of Pc-SNIP1 was significantly up-regulated in hepatopancreas after 20-hydroxyecdysone induction. Knockdown of Pc-SNIP1 gene by small interfering RNA (siRNA) transfection had significant influence on the expression of some ecdysteroid-responsive genes in hepatopancreas, which were confirmed by qRT-PCR and western blot. All together, these results suggest that SNIP1 is involved in the physiological process regulated by ecdysteroid in P. clarkii.
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P.R. China.
authors: Daojun
authors: D
authors: Wang
authors: Tao
authors: T
authors: Peng
authors: Yingying
authors: Y
authors: Yu
authors: Chao-Liang
authors: CL
authors: Liu
authors: Bao-Jian
authors: BJ
authors: Zhu
mesh_terms: D000818:Animals; D003400:Astacoidea; D001483:Base Sequence; D026461:Ecdysteroids / Q000494:pharmacology*; D015870:Gene Expression; D043143:Hepatopancreas / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D015398:Signal Transduction / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D026461:Ecdysteroids; D047908:Intracellular Signaling Peptides and Proteins