title: Conceptual and technical aspects of transfection and gene delivery.
abstract: Genetically modified animals are state of the art in biomedical research as gene therapy is a promising perspective in the attempt to cure hereditary diseases. Both approaches have in common that modified or corrected genetic information must be transferred into cells in general or into particular cell types of an organism. Here we give an overview of established and emerging methods of transfection and gene delivery and provide conceptual and technical advantages and drawbacks of their particular use. Additionally, based on a flow chart, we compiled a rough guideline to choose a gene transfer method for a particular field of application.
authors: Institute for Molecular Cell Biology, Research Centre for Molecular Imaging and Screening, Saarland University, 66421 Homburg/Saar, Germany. Electronic address: lars_kaestner@me.com.
authors: Lars
authors: L
authors: Kaestner
authors: Institute for Molecular Cell Biology, Research Centre for Molecular Imaging and Screening, Saarland University, 66421 Homburg/Saar, Germany.
authors: Anke
authors: A
authors: Scholz
authors: Institute for Molecular Cell Biology, Research Centre for Molecular Imaging and Screening, Saarland University, 66421 Homburg/Saar, Germany.
authors: Peter
authors: P
authors: Lipp
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism; D018014:Gene Transfer Techniques; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism; D000071228:Polyelectrolytes; D014162:Transfection; D014780:Viruses / Q000235:genetics / Q000378:metabolism
chemical_list: D002130:Calcium Phosphates; D057846:Cell-Penetrating Peptides; D050091:Dendrimers; D011073:Polyamines; D000071228:Polyelectrolytes; C020243:calcium phosphate
keywords: Electroporation; Gene therapy; Gene transfer; Lipofection; Transduction