title: Transcriptional response to petiole heat girdling in cassava.
abstract: To examine the interactions of starch and sugar metabolism on photosynthesis in cassava, a heat-girdling treatment was applied to petioles of cassava leaves at the end of the light cycle to inhibit starch remobilization during the night. The inhibition of starch remobilization caused significant starch accumulation at the beginning of the light cycle, inhibited photosynthesis, and affected intracellular sugar levels. RNA-seq analysis of heat-treated and control plants revealed significantly decreased expression of genes related to photosynthesis, as well as N-metabolism and chlorophyll biosynthesis. However, expression of genes encoding TCA cycle enzymes and mitochondria electron transport components, and flavonoid biosynthetic pathway enzymes were induced. These studies reveal a dynamic transcriptional response to perturbation of sink demand in a single leaf, and provide useful information for understanding the regulations of cassava under sink or source limitation.
authors: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan 571101, China.
authors: Yang
authors: Y
authors: Zhang
authors: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan 571101, China.
authors: Zehong
authors: Z
authors: Ding
authors: Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.
authors: Fangfang
authors: F
authors: Ma
authors: Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.
authors: Raj Deepika
authors: RD
authors: Chauhan
authors: 1] Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA [2] United States Department of Agriculture, Agricultural Research Service, Plant Genetic Research Unit, St. Louis, Missouri 63132, USA.
authors: Doug K
authors: DK
authors: Allen
authors: Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.
authors: Thomas P
authors: TP
authors: Brutnell
authors: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan 571101, China.
authors: Wenquan
authors: W
authors: Wang
authors: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan 571101, China.
authors: Ming
authors: M
authors: Peng
authors: 1] The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan 571101, China [2] College of Agronomic Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors: Pinghua
authors: P
authors: Li
mesh_terms: D002874:Chromosome Mapping; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D006358:Hot Temperature; D002365:Manihot / Q000502:physiology*; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology; D015398:Signal Transduction; D013213:Starch / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors; D013395:Sucrose; D013213:Starch