title: Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E.
abstract: We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Silvia
authors: S
authors: Ratto-Kim
authors: United States Military HIV Research Program/United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, 10400, Thailand.
authors: Mark S
authors: MS
authors: de Souza
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Jeffrey R
authors: JR
authors: Currier
authors: United States Military HIV Research Program/United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, 10400, Thailand.
authors: Nicos
authors: N
authors: Karasavvas
authors: La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, United States of America.
authors: John
authors: J
authors: Sidney
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Morgane
authors: M
authors: Rolland
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Anais
authors: A
authors: Valencia-Micolta
authors: United States Military HIV Research Program/United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, 10400, Thailand.
authors: Sirinan
authors: S
authors: Madnote
authors: La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, United States of America.
authors: Alessandro
authors: A
authors: Sette
authors: Thai Component, Armed Forces Research Institute of Medical Sciences, Bangkok, 10400, Thailand.
authors: Sorachai
authors: S
authors: Nitayaphan
authors: Vaccine Trials Centre, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors: Punnee
authors: P
authors: Pitisuttuthum
authors: Centre of Excellence for Biomedical and Public Health Informatics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors: Jaranit
authors: J
authors: Kaewkungwal
authors: Department of Disease Control, Ministry of Public Health, Nonthaburi, 11000, Thailand.
authors: Supachai
authors: S
authors: Rerks-Ngarm
authors: United States Military HIV Research Program/United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, 10400, Thailand.
authors: Robert
authors: R
authors: O'Connell
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Nelson
authors: N
authors: Michael
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Merlin L
authors: ML
authors: Robb
authors: Office of AIDS Research, National Institutes of Health, Bethesda, MD, 20892, United States of America.
authors: Mary
authors: M
authors: Marovich
authors: United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, United States of America.
authors: Jerome H
authors: JH
authors: Kim
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D001666:Binding Sites, Antibody / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D018604:Epitope Mapping; D005260:Female; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D008297:Male; D055815:Young Adult
chemical_list: D016915:AIDS Vaccines; C112734:AIDSVAX; C574872:AIDSVAX B-E; D015483:HIV Antibodies; D016056:Immunodominant Epitopes