title: miR expression in MYC-negative DLBCL/BL with partial trisomy 11 is similar to classical Burkitt lymphoma and different from diffuse large B-cell lymphoma.
abstract: Fast and reliable differential diagnosis of Burkitt lymphoma (BL) vs. diffuse large B cell lymphoma (DLBCL) is of major importance for therapeutic decisions and patient outcome. Aggressive B cell non-Hodgkin lymphomas (B-NHLs) that do not belong to the abovementioned entities were categorized by the current WHO lymphoma classification as "B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and BL" (DLBCL/BL). We have recently described a DLBCL/BL subgroup with recurrent chromosome 11q aberrations, resembling BL (B-NHLs[11q]). Here, we analyzed 102 prospectively collected fine needle aspirates from patients with aggressive B-NHLs in order to investigate the potential of microRNA (miR)-155, its precursor BIC, as well as miR-21 and miR-26a to differentiate BL from DLBCL, and from DLBCL/BL that include B-NHLs[11q]. Both BL and DLBCL/BL cases, including B-NHLs[11q], demonstrated significantly lower expression levels of miR-155/BIC, miR-21, and miR-26a compared to primary DLBCL. In conclusion, the miRs expression in B-NHLs[11q] provides a new suggestion, in addition to pathomorphological and clinical similarities between classical, i.e., MYC translocation-positive BL, and B-NHLs[11q], to recognize the B-NHLs[11q] subgroup of DLBCL/BL category as a MYC translocation-negative variant of BL in most cases, and points to the potential utility of miR-155/BIC/miR-21/miR-26a for the differential diagnosis of a heterogeneous category of DLBCL/BL.
authors: Department of Immunology, Maria Sklodowska-Curie Memorial Centre and Institute of Oncology, Roentgen 5, 02-781, Warsaw, Poland.
authors: Michalina
authors: M
authors: Zajdel
authors: Grzegorz
authors: G
authors: Rymkiewicz
authors: Magdalena
authors: M
authors: Chechlinska
authors: Katarzyna
authors: K
authors: Blachnio
authors: Barbara
authors: B
authors: Pienkowska-Grela
authors: Beata
authors: B
authors: Grygalewicz
authors: Krzysztof
authors: K
authors: Goryca
authors: Maria
authors: M
authors: Cieslikowska
authors: Zbigniew
authors: Z
authors: Bystydzienski
authors: Pawel
authors: P
authors: Swoboda
authors: Jan
authors: J
authors: Walewski
authors: Jan Konrad
authors: JK
authors: Siwicki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D002051:Burkitt Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D014178:Translocation, Genetic; D014314:Trisomy / Q000235:genetics
chemical_list: C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc