title: Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis.
abstract: Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb.
authors: a Institute Finlay ; La Habana , Cuba.
authors: Yanely
authors: Y
authors: Tirado
authors: Alina
authors: A
authors: Puig
authors: Nadine
authors: N
authors: Alvarez
authors: Reinier
authors: R
authors: Borrero
authors: Alicia
authors: A
authors: Aguilar
authors: Frank
authors: F
authors: Camacho
authors: Fatima
authors: F
authors: Reyes
authors: Sonsire
authors: S
authors: Fernández
authors: José Luis
authors: JL
authors: Pérez
authors: Dulce Mata
authors: DM
authors: Espinoza
authors: Jorge Alberto Barrios
authors: JA
authors: Payán
authors: María Elena
authors: ME
authors: Sarmiento
authors: Mohd-Nor
authors: MN
authors: Norazmi
authors: Rogelio
authors: R
authors: Hernández-Pando
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D058491:Bacterial Load; D004195:Disease Models, Animal; D008168:Lung / Q000382:microbiology; D008297:Male; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000737:chemistry / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D011510:Proteolipids / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D011510:Proteolipids; D032581:Tuberculosis Vaccines; C020485:proteoliposomes; C041524:aluminum sulfate
keywords: BCG; BCG, Bacillus Calmette-Guerin; CFU, Colony Forming Unit; Mtb, Mycobacterium tuberculosis; PBS, Phosphate Buffer Solution; PL Proteoliposomes; PLBCG, Proteoliposomes obtained from BCG; PLBCG-Al, PLBCG adjuvanted with alum; SD, standard deviation; TB, Tuberculosis; Tuberculosis; prime-boost; proteoliposomes; vaccines