title: Flow cytometric evaluation of disseminated intravascular coagulation in a canine endotoxemia model.
abstract: Sepsis is associated with substantial morbidity and mortality in dogs. Alterations in hemostasis by systemic inflammation play an important role in the pathophysiology of sepsis. To evaluate the functional hemostatic changes in sepsis, we evaluated coagulation profiles and flow cytometric measurement of P-selectin (CD62P) expression on platelets, as well as platelet-leukocyte aggregation from a lipopolysaccharide (LPS)-induced endotoxemia model in dogs (n = 7). A sublethal dose of LPS [1 mg/kg body weight (BW)] induced thrombocytopenia and increased activated partial thromboplastin time (aPTT), prothrombin time (PT), and D-dimer concentrations. Flow cytometry analysis showed a significant increase in P-selectin expression on platelets between 1 and 24 h of a total 48 h of the experiment. In addition, platelet-leukocyte aggregation was significantly increased in the early stage of endotoxemia (at 1 and < 6 h for platelet-monocyte aggregation and at 3 h for platelet-neutrophil aggregation). Our results suggest that CD62P expression on platelets and platelet-leukocyte aggregation, as measured by flow cytometry, can be useful biomarkers of disseminated intravascular coagulation (DIC) in canine sepsis. These functional changes contribute to our understanding of the pathophysiology of hemostasis in endotoxemia.
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757 (Yu), and Chonbuk National University, Jeonju, 561-756 (Noh, Park), Korea.
authors: Dohyeon
authors: D
authors: Yu
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757 (Yu), and Chonbuk National University, Jeonju, 561-756 (Noh, Park), Korea.
authors: Dongho
authors: D
authors: Noh
authors: College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757 (Yu), and Chonbuk National University, Jeonju, 561-756 (Noh, Park), Korea.
authors: Jinho
authors: J
authors: Park
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D004285:Dogs; D019446:Endotoxemia / Q000150:complications / Q000662:veterinary*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005434:Flow Cytometry; D008070:Lipopolysaccharides; D008297:Male; D019007:P-Selectin / Q000097:blood*; D010974:Platelet Aggregation
chemical_list: D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; D008070:Lipopolysaccharides; D019007:P-Selectin; C036309:fibrin fragment D; C440499:lipopolysaccharide, Escherichia coli O111 B4