title: Determination of zinc pyrithione in shampoos by HPLC and HPLC-MS/MS.
abstract: Methods have been developed for the determination of zinc pyrithione (ZPT) in shampoos using high-performance liquid chromatography (HPLC) and high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS). Samples were washed by water first to remove surfactant and water-soluble impurities, then ultrasonic-extracted by acetonitrile-methanol for 30 min, and finally analyzed by MG C18 column (250 mm x 4.6 mm, 5 μm) or RP-18e (100 mm x 3 mm, 2 μm) plus APCI-MS/MS. Limits of detection were determined as 0.015% (HPLC) and 0.003% (HPLC-MS/MS), with a limit of quantization of 0.05% and 0.01%, respectively. The recoveries were 85.8-104% (HPLC) and 87.6-107% (HPLC-MS/MS). A good linear relationship was obtained from 3.20 μg·ml(-1) to 200 μg·ml(-1) (HPLC) and 1.00 μg·ml(-1) to 200 μg·ml(-1) (HPLC-MS/MS). The proposed methods have been successfully applied to the analysis of ZPT in many shampoos. The established two methods were rapid and reproducible with low interference.
authors: Yu-Xiang
authors: YX
authors: Gu
authors: Qing-He
authors: QH
authors: Wang
authors: Ze-Lin
authors: ZL
authors: Zhou
authors: Qing
authors: Q
authors: Lv
authors: Cheng-Hua
authors: CH
authors: Mai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006203:Hair Preparations / Q000737:chemistry*; D057230:Limit of Detection; D009942:Organometallic Compounds / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006203:Hair Preparations; D009942:Organometallic Compounds; D011725:Pyridines; C010423:pyrithione zinc