title: Formulation of nanoliposomal vitamin d3 for potential application in beverage fortification.
abstract: OBJECTIVE
  Vitamin D, a liposoluble vitamin has many benefits on health. Encapsulation of bioactives in lipid-based carrier systems like nanoliposomes preserves their native properties against oxidation over time along with providing its stable aqueous dispersion.
  
  
  METHODS
  In the current study, vitamin D3 nanoliposomes were prepared using thin-film hydration-sonication method and fully characterized by different instrumental techniques.
  
  
  RESULTS
  According to FTIR and DSC results, no interaction was observed between encapsulated nutraceutical and liposome constituents. The particle size and size distribution (Span value) were calculated 82-90 nm and 0.70-0.85, respectively. TEM analysis showed nano sized globular and bilayer vesicles. In all formations, the encapsulation efficiency of vitamin D3 was calculated more than 93%. Addition of cholesterol to lecithin bilayer increased the negative zeta potential from -29 to -43mV.
  
  
  CONCLUSIONS
  The results of this study concluded that the liposomal nanoparticles may be introduced as a suitable carrier for fortification of beverages with vitamin D3.
authors: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
authors: Maryam
authors: M
authors: Mohammadi
authors: Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
authors: Babak
authors: B
authors: Ghanbarzadeh
authors: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Hamed
authors: H
authors: Hamishehkar
keywords: Fortification; Nanoliposomes; Nutraceutical; Vitamin D3