title: Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design.
abstract: The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E) on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolactone yield. In order to determine the optimal combination of the four factors viz. operating pressure, temperature, modifier concentration and extraction time, a Taguchi experimental design approach was used: four variables (three levels) in L9 orthogonal array. Wedelolactone content was determined using validated HPLC methodology. Optimum extraction conditions were found to be as follows: extraction pressure, 25 MPa; temperature, 40 °C; modifier concentration, 10% and extraction time, 90 min. Optimum extraction conditions demonstrated wedelolactone yield of 8.01 ± 0.34 mg/100 g W. calendulacea Less. Pressure, temperature and time showed significant (p < 0.05) effect on the wedelolactone yield. The supercritical carbon dioxide extraction showed higher selectivity than the conventional Soxhlet assisted extraction method.
authors: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, MH, India.
authors: Ajit A
authors: AA
authors: Patil
authors: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, MH, India.
authors: Bhusari S
authors: BS
authors: Sachin
authors: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, MH, India.
authors: Pravin S
authors: PS
authors: Wakte
authors: Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, MH, India.
authors: Devanand B
authors: DB
authors: Shinde
keywords: CAL STDs, calibration standards; Diff%, % difference; HQC, high quality control; High-performance liquid chromatography; LQC, low quality control; MQC, medium quality control; QC STDs, quality control standards; SAE, Soxhlet assisted extraction; SC CO2E, supercritical carbon dioxide assisted extraction; Supercritical carbon dioxide extraction; Taguchi orthogonal array design; Wedelia calendulacea; Wedelolactone