title: Implanted scaffold-free prevascularized constructs promote tissue repair.
abstract: To evaluate the anastomotic potential of prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells, tissue constructs were implanted into athymic mice and immune-competent rats. Analysis of xenografts placed into hind limb muscle defects showed vascular anastomotic activity by 3 days after implantation and persisting for 2 weeks. Integration of the implanted prevascular tissue constructs with the host circulatory system was evident from presence of red blood cells in the implant as early as 3 days after implantation. Additionally, analysis of 3-day xenografts in the rat model showed activation of skeletal muscle satellite cells based on Pax-7 and MyoD expressions. We conclude that prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells are a promising tool to provide both vascular supply and satellite cell activation toward the resolution of skeletal muscle injury.
authors: From the *Department of Regenerative Medicine and Cell Biology, †Department of Surgery, Medical University of South Carolina, Charleston, SC.
authors: Caitlin A
authors: CA
authors: Czajka
authors: Bennet W
authors: BW
authors: Calder
authors: Michael J
authors: MJ
authors: Yost
authors: Christopher J
authors: CJ
authors: Drake
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018482:Muscle, Skeletal / Q000098:blood supply / Q000293:injuries* / Q000473:pathology / Q000502:physiology; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D032496:Satellite Cells, Skeletal Muscle / Q000473:pathology / Q000502:physiology; D017695:Soft Tissue Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014945:Wound Healing