title: Inhibitory effects of lupene-derived pentacyclic triterpenoids from Bursera simaruba on HSV-1 and HSV-2 in vitro replication.
abstract: The cytotoxicity and antiviral properties of Bursera simaruba against herpes simplex viruses (HSV-1 and HSV-2) were investigated through a bioactivity-guided isolation protocol. The plant material was fractionated using solvent-solvent partitioning, size-exclusion and thin-layer chromatography. The antiviral compounds present in the most active fractions were identified by means of LC-MS and NMR. Three different methods were compared during the evaluation of antiviral activity of samples. Four lupene-related pentacyclic triterpenes were found to be responsible for the anti-herpesvirus effects of B. simaruba and were isolated from this species for the first time. The selective indexes (SI) of B. simaruba-derived samples ranged from 7.7 to 201.9.
authors: a Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo , Oviedo , 33006 Asturias , Spain.
authors: Ángel L
authors: ÁL
authors: Álvarez
authors: b Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich , Central Avenue, Chatham-Maritime, Kent ME4 4TB , UK.
authors: Solomon
authors: S
authors: Habtemariam
authors: a Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo , Oviedo , 33006 Asturias , Spain.
authors: Francisco
authors: F
authors: Parra
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D031233:Bursera / Q000737:chemistry*; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes / Q000737:chemistry* / Q000302:isolation & purification; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D053978:Pentacyclic Triterpenes
keywords: Bursera simaruba; HSV-1; HSV-2; antiviral; pentacyclic triterpenoids