title: Solubilized extracellular matrix from brain and urinary bladder elicits distinct functional and phenotypic responses in macrophages.
abstract: Extracellular matrix (ECM) derived from a variety of source tissues has been successfully used to facilitate tissue reconstruction. The recent development of solubilized forms of ECM advances the therapeutic potential of these biomaterials. Isolated, soluble components of ECM and matricryptic peptides have been shown to bias macrophages toward a regulatory and constructive (M2-like) phenotype. However, the majority of studies described thus far have utilized anatomically and morphologically similar gastrointestinal derived ECMs (small intestine, esophagus, urinary bladder, etc.) and a small subset of macrophage markers (CD206, CD86, CCR7) to describe them. The present study evaluated the effect of solubilized ECM derived from molecularly diverse source tissues (brain and urinary bladder) upon primary macrophage phenotype and function. Results showed that solubilized urinary bladder ECM (U-ECM) up-regulated macrophage PGE2 secretion and suppressed traditional pro-inflammatory factor secretion, consistent with an M2-like phenotype. The hyaluronic acid (HA) component in solubilized U-ECM played an important role in mediating this response. Brain ECM (B-ECM) elicited a pro-inflammatory (M1-like) macrophage response and contained almost no HA. These findings suggest that the molecular composition of the source tissue ECM plays an important role in influencing macrophage function and phenotype.
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, 15219 PA, USA.
authors: Fan Wei
authors: FW
authors: Meng
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, 15219 PA, USA.
authors: Peter F
authors: PF
authors: Slivka
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, 15219 PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, 15219 PA, USA; DoD-VA Extremity Trauma & Amputation Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA.
authors: Christopher L
authors: CL
authors: Dearth
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, 15219 PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, 15219 PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, 15219 PA, USA. Electronic address: badylaks@upmc.edu.
authors: Stephen F
authors: SF
authors: Badylak
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis; D010641:Phenotype; D012995:Solubility; D034421:Sus scrofa; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D001119:Arginase; D015232:Dinoprostone
keywords: Cyclooxygenase; Extracellular matrix; Hyaluronic acid; Hydrogel; Macrophage