title: Shark cartilage 14 kDa protein as a dendritic cells activator.
abstract: Low molecular weight components of shark cartilage are reported to have anti-tumor as well as immuno-stimulating effects. Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that have a key role in establishment of anti-cancer immune response. In this study, the effect of 14 kDa protein from shark cartilage was investigated on stimulation and maturation of dendritic cells. The isolated 14 kDa protein from shark cartilage extract was added to DCs medium during overnight culture and their maturation and T cells stimulation potential was investigated. The majority of shark-cartilage-treated DCs expressed higher levels of maturation markers and were more effective in stimulation of allogenic T cells compared with non-treated DCs (p < 0.05). Our results showed that shark cartilage 14 kDa protein can potentially be used in DC-mediated T-cells stimulation and induction of desirable immune responses in clinical trials such as cancer immunotherapy. However, further studies are required to examine this proposal.
authors: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
authors: Elahe
authors: E
authors: Safari
authors: Zuhair M
authors: ZM
authors: Hassan
authors: Seyed Mohammad
authors: SM
authors: Moazzeni
mesh_terms: D000818:Animals; D002356:Cartilage; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D004284:Dogfish; D064235:Matrilin Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D064235:Matrilin Proteins
keywords: Allogenic MLR; T cell stimulation; dendritic cell; shark cartilage; tumor