title: Matrix-free DNP-enhanced NMR spectroscopy of liposomes using a lipid-anchored biradical.
abstract: Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.
authors: Université Grenoble Alpes, INAC, LCIB, 38000 Grenoble (France), Fax: (+33) 4-38-78-50-90; CEA, INAC, LCIB, 38054 Grenoble (France).
authors: Carlos
authors: C
authors: Fernández-de-Alba
authors: Hiroki
authors: H
authors: Takahashi
authors: Alexandre
authors: A
authors: Richard
authors: Yves
authors: Y
authors: Chenavier
authors: Lionel
authors: L
authors: Dubois
authors: Vincent
authors: V
authors: Maurel
authors: Daniel
authors: D
authors: Lee
authors: Sabine
authors: S
authors: Hediger
authors: Gaël
authors: G
authors: De Paëpe
mesh_terms: D003497:Cyclic N-Oxides / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D020005:Propanols / Q000737:chemistry
chemical_list: C521769:1-(TEMPO-4-oxy)-3-(TEMPO-4-amino)propan-2-ol; D003497:Cyclic N-Oxides; D005609:Free Radicals; D008051:Lipid Bilayers; D008081:Liposomes; D020005:Propanols
keywords: NMR spectroscopy; dynamic nuclear polarization; membranes; phospholipids; solid-state NMR spectroscopy