title: miR-1271 promotes non-small-cell lung cancer cell proliferation and invasion via targeting HOXA5.
abstract: MicroRNAs (miRNAs) are short, non-coding RNAs (∼ 22 nt) that play important roles in the pathogenesis of human diseases by negatively regulating numerous target genes at posttranscriptional level. However, the role of microRNAs in lung cancer, particularly non-small-cell lung cancer (NSCLC), has remained elusive. In this study, two microRNAs, miR-1271 and miR-628, and their predicted target genes were identified differentially expressed in NSCLC by analyzing the miRNA and mRNA expression data from NSCLC tissues and their matching normal controls. miR-1271 and its target gene HOXA5 were selected for further investigation. CCK-8 proliferation assay showed that the cell proliferation was promoted by miR-1271 in NSCLC cells, while miR-1271 inhibitor could significantly inhibited the proliferation of NSCLC cells. Interestingly, migration and invasion assay indicated that overexpression of miR-1271 could significantly promoted the migration and invasion of NSCLC cells, whereas miR-1271 inhibitor could inhibited both cell migration and invasion of NSCLC cells. Western blot showed that miR-1271 suppressed the protein level of HOXA5, and luciferase assays confirmed that miR-1271 directly bound to the 3'untranslated region of HOXA5. This study indicated indicate that miR-1271 regulates NSCLC cell proliferation and invasion, via the down-regulation of HOXA5. Thus, miR-1271 may represent a potential therapeutic target for NSCLC intervention.
authors: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University, Changzhou 213002, China.
authors: Yongfang
authors: Y
authors: Wang
authors: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University, Changzhou 213002, China.
authors: Lianhong
authors: L
authors: Xu
authors: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University, Changzhou 213002, China.
authors: Lixin
authors: L
authors: Jiang
mesh_terms: D020413:3' Untranslated Regions; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C489213:HOXA5 protein, human; D018398:Homeodomain Proteins; C556309:MIRN1271 microRNA, human; C000589022:MIRN628 microRNA, human; D035683:MicroRNAs
keywords: Non-small-cell lung cancer; Proliferation; miR-1271