title: Arsenite oxidation by Pseudomonas arsenicoxydans immobilized on zeolite and its potential biotechnological application.
abstract: Pseudomonas arsenicoxydans has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS. P. arsenicoxydans showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg arsenite present in the medium after 48 of incubation). Arsenite oxidizing activity, mediated by a constitutive periplasmic enzyme, was determined following the transfer of reducing equivalents from arsenite to 2,4-dichlorophenolindophenol (DCIP) showing that approximately 75 % (0.173 µmol DCIP min(-1) mg(-1)) of the total activity (0.231 µmol DCIP min(-1) mg(-1)) was detected in the periplasmic fraction. Using PCR with primers specific for arsenite oxidase gene showed the presence of a gene encoding for arsenite oxidase in P. arsenicoxydans. Results show the potential biotechnological application of P. arsenicoxydans as a candidate for detoxification of As(III).
authors: Environmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepcion, P.O. Box 160-C, Correo 3, Concepción, Chile.
authors: Cristian
authors: C
authors: Valenzuela
authors: Ruben
authors: R
authors: Moraga
authors: Carla
authors: C
authors: Leon
authors: Carlos T
authors: CT
authors: Smith
authors: Maria-Angelica
authors: MA
authors: Mondaca
authors: Victor L
authors: VL
authors: Campos
mesh_terms: D018053:Arsenites / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017641:Zeolites / Q000737:chemistry*
chemical_list: D018053:Arsenites; D001426:Bacterial Proteins; D004785:Environmental Pollutants; D017641:Zeolites; D010088:Oxidoreductases; C078179:arsenite oxidase; C015001:arsenite