title: [Expression of CD71 on cell proliferation in hematologic malignancy and its correlation with Ki-67].
abstract: OBJECTIVE
  This study was to explore the expression of CD71, as a proliferation indicator, on cell proliferaration in hematologic malignancy and its correlation with Ki-67, so as to assess the feasibility of CD71 instead of Ki-67 for assaying cell proliferation by flow cytometry (FCM).
  
  
  METHODS
  (1) Compared with mature B lymphoctyes during stationary phase in peripheral blood from healthy people, the cell cycle and the expression of CD71 and Ki-67 of cell lines from patients with leukemia and lymphoma were examined, the correlation among CD71, S-phase cell fraction (SPF) and Ki-67 were analyzed; (2) Compared with mature B lymphoctyes in bone marrow from non-hematologic disease patients, the expression and correlation of CD71 and Ki-67 of all kinds of leukemic cells and myeloma cells from bone marrow were analyzed by using Ki-67/CD71/CD45/CD123, Ki-67/CD71/CD45/CD20 or Ki-67/CD71/CD45/CD138.
  
  
  RESULTS
  (1) in respect to the expression rate of CD71 on tumor cell lines, the expression rate of CD71 on HL-60 cells was (99.77 ± 0.064)%, the expression rate of CD71 on NB4 cells was (99.23 ± 0.12)%, the expression rate on THP-1 cells was (98.90 ± 0.30)% and the expression rate on K562 cells was (97.03 ± 0.15)% in myelogenous leukemia cell lines, the expression rate of CD71 on Raji cells was (99.35 ± 0.21)% and the expression rate on Mino cell was (96.95 ± 0.42)% in lymphoma cell lines, which were also obviously higher than that on cells of the control group (P < 0.05); (2) in respect to the expression rate of CD71 on tumor cells in bone marrow, the expression rate of CD71 on poorly differentiated AML(M1 and M2) cells was (51.50 ± 19.31)%, the expression rate of CD71 on acute promyelocytic leukemia (AML-M3) cells was (35.71 ± 14.02) %, the expression rate of CD71 on acute monocytic leukemia (AML-M5) cells was (30.54 ± 14.38)%, the expression rate of CD71 on acute T lymphoblastic leukemia cells was (68.40 ± 20.83)%, the expression rate of CD71 on acute B lymphoblastic leukemia was (39.67 ± 18.27)%, the expression rate of CD71 on multiple myeloma (MM) cells was (55.49 ± 18.15%), the expression rate of CD71 on chronic lymphocytic leukemia(CLL) was (1.32 ± 0.33%), which were also higher than that on cells in the control group(P < 0.05) except for CLL cells (P > 0.05); (3) CD71 had a positive linear corrlation with SPF in cell lines (r = 0.914, P < 0.05), and also had a positive linear corrlation with Ki-67 in cell lines and carcinoma cells from bone marrow (r = 0.894,r = 0.904, P < 0.05).
  
  
  CONCLUSIONS
  The CD71 can take the place of Ki-67 as an indicator of cell proliferation activity of hematologic malignancies and the determination CD71 by FCM is simpler and better than that of Ki-67 in respest of methodology.
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Yuan-Yu
authors: YY
authors: Wei
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Xiao-Zhan
authors: XZ
authors: Zhang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Fan
authors: F
authors: Zhang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Zhao-Bo
authors: ZB
authors: Li
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Ning-Ning
authors: NN
authors: Wang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors: Shuai
authors: S
authors: Liu
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Faculty of Laboratorial Medicine of Zhengzhou Uniersity, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China. E-mail:ybh2002@163.com.
authors: Bao-Hong
authors: BH
authors: Yue
mesh_terms: D015703:Antigens, CD; D002455:Cell Division; D049109:Cell Proliferation; D005434:Flow Cytometry; D019337:Hematologic Neoplasms; D006801:Humans; D019394:Ki-67 Antigen; D011990:Receptors, Transferrin
chemical_list: D015703:Antigens, CD; C068126:CD71 antigen; D019394:Ki-67 Antigen; D011990:Receptors, Transferrin