title: IL-1B (C+3954T) gene polymorphism and susceptibility to gastric cancer in the Iranian population.
abstract: BACKGROUND
  Gastric cancer as one of the most important diseases affecting health in all worldwide. Current studies have confirmed associations of cytokine gene polymorphisms with the risk of gastric cancer development. The current research aimed to assess the association of IL-1B+3954 genotypes with the risk of gastric cancer in the Iranian population.
  
  
  METHODS
  This case-control study covered 49 gastric cancer patients compared to 53 cancer free individuals as a control group. Genomic-DNA extraction was carried out from bioptic samples of patients and peripheral blood of healthy volunteers. Polymorphism of IL-1B +3954 genotypes were analysed with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  The frequencies of IL-1B +3954 A1A1, A1A2 and A2A2 genotypes in healthy individuals were 26.4, 66 and 7.6 %, respectively. However, in gastric cancer patients, A1A1, A1A2 and A2A2 with 4.1, 51 and 44.9% were observed (p<0.05).
  
  
  CONCLUSIONS
  The findings of our results show a positive association between the IL-1B+3954 genotype distribution and the risk of gastric cancer disease in the Iranian population.
authors: Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Kermanshah, Iran E-mail : ismaili.a@lu.ac.ir; ahmad_ismaili@yahoo.com.
authors: Ahmad
authors: A
authors: Ismaili
authors: Kheirollah
authors: K
authors: Yari
authors: Mohammad-Taher
authors: MT
authors: Moradi
authors: Maryam
authors: M
authors: Sohrabi
authors: Danial
authors: D
authors: Kahrizi
authors: Elham
authors: E
authors: Kazemi
authors: Zahra
authors: Z
authors: Souri
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics*
chemical_list: D053583:Interleukin-1beta