title: Molecular characterization, tissue distribution and expression analysis of TRIM25 in Gallus gallus domesticus.
abstract: TRIM25, a member of the tripartite motif-containing (TRIM) family of proteins, plays an important role in cell proliferation, protein modification, and the RIG-I-mediated antiviral signaling pathway. However, relatively few studies have investigated the molecular characterization, tissue distribution, and potential function of TRIM25 in chickens. In this study, we cloned the full-length cDNA of chicken TRIM25 that is composed of 2706 bp. Sequence analyses revealed that TRIM25 contains a 1902-bp open-reading frame that probably encodes a 633-amino acid protein. Multiple comparisons with deduced amino acid sequences revealed that the RING finger and B30.2 domains of chicken TRIM25 share a high sequence similarity with human and murine TRIM25, indicating that these domains are critical for the function of chicken TRIM25. qPCR assays revealed that TRIM25 is highly expressed in the spleen, thymus and lungs in chickens. Furthermore, we observed that TRIM25 expression was significantly upregulated both in vitro and in vivo following infection with Newcastle disease virus. TRIM25 expression was also significantly upregulated in chicken embryo fibroblasts upon stimulation with poly(I:C) or poly(dA:dT). Taken together, these findings suggest that TRIM25 plays an important role in antiviral signaling pathways in chickens.
authors: College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
authors: Ze-Qing
authors: ZQ
authors: Feng
authors: College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
authors: Yang
authors: Y
authors: Cheng
authors: College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
authors: Hui-Ling
authors: HL
authors: Yang
authors: College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
authors: Qing
authors: Q
authors: Zhu
authors: Key laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan 650223, China.
authors: Dandan
authors: D
authors: Yu
authors: College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan 625014, China. Electronic address: liuyp578@yahoo.com.
authors: Yi-Ping
authors: YP
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001483:Base Sequence; D049109:Cell Proliferation / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000235:genetics* / Q000821:virology; D009522:Newcastle disease virus; D011061:Poly A / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D011071:Poly T / Q000494:pharmacology; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D004268:DNA-Binding Proteins; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors; D011061:Poly A; D011071:Poly T; C008950:poly A-T; D044767:Ubiquitin-Protein Ligases; D011070:Poly I-C
keywords: Chicken; Innate immune; Tripartite motif protein 25; mRNA expression