title: Myeloperoxidase-deficient zebrafish show an augmented inflammatory response to challenge with Candida albicans.
abstract: Myeloperoxidase is a key component of neutrophil granules involved in killing engulfed microorganisms. We obtained a zebrafish mutant (smu681) lacking Sudan black staining by large-scale screening, which was a neutrophil-replete but myeloperoxidase-deficient mutant. When infiltrated with Candida albicans, smu681 embryos and sibling embryos showed similar survival after infection. Proliferation of C. albicans was more rapid in smu681 embryos than in sibling embryos, although it was eventually suppressed. In addition, the number of neutrophils accumulating at the site of infection was significantly larger in mutant embryos than in sibling embryos, and mutant embryos showed increased expression of several inflammatory cytokines after C. albicans infection. These findings indicate that myeloperoxidase deficiency alters the inflammatory response to fungal infection.
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Kun
authors: K
authors: Wang
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Xiao
authors: X
authors: Fang
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Ning
authors: N
authors: Ma
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Qing
authors: Q
authors: Lin
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Zhibin
authors: Z
authors: Huang
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Wei
authors: W
authors: Liu
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Mengchang
authors: M
authors: Xu
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China.
authors: Xiaohui
authors: X
authors: Chen
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China. Electronic address: zzwwqq@smu.edu.cn.
authors: Wenqing
authors: W
authors: Zhang
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, PR China. Electronic address: yiyue@smu.edu.cn.
authors: Yiyue
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology / Q000502:physiology*; D004195:Disease Models, Animal; D029941:Fish Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D009154:Mutation; D009195:Peroxidase / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*
chemical_list: D029941:Fish Proteins; D009195:Peroxidase
keywords: Fungal infection; Inflammation; Neutrophil; Zebrafish myeloperoxidase