title: Protective effect of ATP on skeletal muscle satellite cells damaged by H₂O₂.
abstract: This study investigated the protective effect of ATP on skeletal muscle satellite cells damaged by H₂O₂in neonatal rats and the possible mechanism. The skeletal muscle satellite cells were randomly divided into four groups: normal group, model group (cells treated with 0.1 mmol/L H₂O₂for 50 s), protection group (cells treated with 16, 8, 4, 2, 1, 0.5, or 0.25 mmol/L ATP for 24 h, and then with 0.1 mmol/L H₂O₂for 50 s), proliferation group (cells treated with 16, 8, 4, 2, 1, 0.5, or 0.25 mmol/L ATP for 24 h). MTT assay, FITC+PI+DAPI fluorescent staining, Giemsa staining and immunofluorescence were performed to examine cell viability and apoptosis, and apoptosis-related proteins. The results showed that the survival rate of skeletal muscle satellite cells was decreased and the apoptosis rate was increased after H₂O₂treatment (P<0.01). Different doses of ATP had different effects on skeletal muscle satellite cells damaged by H₂O₂: the survival rate of muscle satellite cells treated with ATP at 4, 2, or 1 mmol/L was increased. The protective effect was most profound on cells treated with 2 mmol/L ATP. Immunofluorescence showed that ATP could increase the number of Bcl-2-positive cells (P<0.01) and decrease the number of the Bax-positive cells (P<0.01). It was concluded that ATP could protect skeletal muscle satellite cells against H₂O₂damage in neonatal rats, which may be attributed to the up-regulation of the expression of Bcl-2 and down-regulation of Bax, resulting in the suppression of apoptosis.
authors: Department of Biology, School of Life Science, Nantong University, Nantong, 226019, China.
authors: Fei
authors: F
authors: Fei
authors: Department of Biology, School of Life Science, Nantong University, Nantong, 226019, China. zhudaoli@ntu.edu.cn.
authors: Dao-Li
authors: DL
authors: Zhu
authors: Department of Biology, School of Life Science, Nantong University, Nantong, 226019, China.
authors: Li-Jun
authors: LJ
authors: Tao
authors: Department of Biology, School of Life Science, Nantong University, Nantong, 226019, China.
authors: Bao-Zhu
authors: BZ
authors: Huang
authors: Department of Biology, School of Life Science, Nantong University, Nantong, 226019, China.
authors: Hong-Hong
authors: HH
authors: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects*
chemical_list: D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide