title: The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis.
abstract: FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2 ). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis.
authors: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Germany.|Department of Bioprocessing and Biotechnology, School of Pharmaceutical Sciences, Univ Estadual Paulista - UNESP, Araraquara, Brazil.
authors: Danielle
authors: D
authors: Pedrolli
authors: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Germany.
authors: Simone
authors: S
authors: Langer
authors: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Germany.
authors: Birgit
authors: B
authors: Hobl
authors: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Germany.
authors: Julia
authors: J
authors: Schwarz
authors: Institute of Molecular Medicine, Medical School, National Chengkung University, Tainan City, Taiwan.|Center for Infectious Disease and Signal Transduction, Medical School, National Chengkung University, Tainan City, Taiwan.
authors: Masayuki
authors: M
authors: Hashimoto
authors: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Germany.
authors: Matthias
authors: M
authors: Mack
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012256:Riboflavin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D058928:Riboswitch / Q000235:genetics*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D020121:5' Untranslated Regions; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C534627:Rho protein, E coli; D058928:Riboswitch; C576819:roseoflavin mononucleotide; C071147:ribB protein, E coli; D005486:Flavin Mononucleotide; D019751:Intramolecular Transferases; C024504:bicozamycin; D012256:Riboflavin
keywords: Escherichia coli; FMN riboswitch; Streptomyces davawensis; riboflavin biosynthesis; roseoflavin