title: Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma.
abstract: Recent evidence suggested that nonirradiated cancer-associated fibroblasts (CAFs) promoted aggressive phenotypes of cancer cells through epithelial-mesenchymal transition (EMT). Hepatoma-derived growth factor (HDGF) is a radiosensitive gene of esophageal squamous cell carcinoma (ESCC). This study aimed to investigate the effect of irradiated fibroblasts on EMT and HDGF expression of ESCC. Our study demonstrated that coculture with nonirradiated fibroblasts significantly increased the invasive ability of ESCC cells and the increased invasiveness was further accelerated when they were cocultured with irradiated fibroblasts. Scattering of ESCC cells was also accelerated by the supernatant from irradiated fibroblasts. Exposure of ESCC cells to supernatant from irradiated fibroblasts resulted in decreased E-cadherin, increased vimentin in vitro and β-catenin was demonstrated to localize to the nucleus in tumor cells with irradiated fibroblasts in vivo models. The expression of HDGF and β-catenin were increased in both fibroblasts and ESCC cells of irradiated group in vitro and in vivo models. Interestingly, the tumor cells adjoining the stromal fibroblasts displayed strong nuclear HDGF immunoreactivity, which suggested the occurrence of a paracrine effect of fibroblasts on HDGF expression. These data suggested that irradiated fibroblasts promoted invasion, growth, EMT and HDGF expression of ESCC.
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Ci-Hang
authors: CH
authors: Bao
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Xin-Tong
authors: XT
authors: Wang
authors: Department of Radiation Oncology, Cancer Hospital, Genaral Hospital of Ningxia Medical University, Yinchuan 750000, China.
authors: Wei
authors: W
authors: Ma
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Na-Na
authors: NN
authors: Wang
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Effat
authors: E
authors: Un Nesa
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Jian-Bo
authors: JB
authors: Wang
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Cong
authors: C
authors: Wang
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Yi-Bin
authors: YB
authors: Jia
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Kai
authors: K
authors: Wang
authors: Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan 250012, China.
authors: Hui
authors: H
authors: Tian
authors: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China. Electronic address: qlcyf1965@126.com.
authors: Yu-Feng
authors: YF
authors: Cheng
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002450:Cell Communication / Q000528:radiation effects*; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000528:radiation effects*; D004938:Esophageal Neoplasms / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C051610:hepatoma-derived growth factor
keywords: Epithelial-mesenchymal transition; Esophageal squamous cell carcinoma; Fibroblasts; Hepatoma-derived growth factor; Irradiation