title: Evaluation in vivo of autologous cell derived vein grafts based on tissue engineering concept.
abstract: OBJECTIVE
  Aim of the study was to construct and evaluate of autologous cell derived vein grafts based on tissue engineering concept.
  
  
  METHODS
  In this study, we constructed venous grafts (VGs) in 12 days based on tissue engineering concept. We draw out 8-12 mL of bone marrow from the intended recipient canines (N.=8) to culture and expand endothelial progenitor cells (EPCs). After having been labeled with PKH26-GL, the cells were seeded onto the luminal surface of decellularized scaffolds (DSs) with single, rotative method for 4 hours. Following static culture for 24-72 hours, the hybrids were implanted to recipient canine inferior vena cava. Non-seeded DSs (N.=4) were performed as control.
  
  
  RESULTS
  Angiography disclosed that patent number of test (control) group were 7/7 (2/4), 6/6 (2/2) and 4/4 (1/2) at postoperative 10 days, 4 weeks and 12 weeks, respectively. At 12 weeks, confluenced endothelial cells which covered the whole inner luminal surface of the explants were detected. Meanwhile, fibroblasts and α-actin positive cells in the matrices were found. PKH26-GL labeled EPCs sustained on the luminal surface accompanied by newly formed endothelial cells. However, the explants in both groups showed partial stenosis.
  
  
  CONCLUSIONS
  These results indicate that such constructed VGs based on autologous bone marrow-derived EPCs and porcine DSs are promising and deserve to further improvement and testing.
authors: Deparment of Vascular Surgery, Xuan Wu Hospital and Institute of Vascular Surgery, Capital Medical University, Beijing, China - xwhvs@163.com.
authors: Y F
authors: YF
authors: Wu
authors: F L
authors: FL
authors: He
authors: Y Q
authors: YQ
authors: Gu
authors: X S
authors: XS
authors: Chen
authors: L
authors: L
authors: Chen
authors: L
authors: L
authors: Chen
authors: J
authors: J
authors: Zhang
authors: Z G
authors: ZG
authors: Wang
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis / Q000662:veterinary*; D002478:Cells, Cultured; D004285:Dogs; D066026:Endothelial Progenitor Cells / Q000637:transplantation*; D005260:Female; D008297:Male; D009930:Organic Chemicals / Q000737:chemistry; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014682:Vena Cava, Inferior
chemical_list: D009930:Organic Chemicals; C070080:PKH 26