title: The role of glycerol transporters in yeast cells in various physiological and stress conditions.
abstract: Small and uncharged glycerol is an important molecule for yeast metabolism and osmoadaptation. Using a series of S. cerevisiae BY4741-derived mutants lacking genes encoding a glycerol exporter (Fps1p) and/or importer (Stl1p) and/or the last kinase of the HOG pathway (Hog1p), we studied their phenotypes and various physiological characteristics with the aim of finding new roles for glycerol transporters. Though the triple mutant hog1Δ stl1Δ fps1Δ was viable, it was highly sensitive to various stresses. Our results showed that the function of both Stl1p and Fps1p transporters contributes to the cell ability to survive during the transfer into the state of anhydrobiosis, and that the deletion of FPS1 decreases the cell's tolerance of hyperosmotic stress. The deletion of STL1 results in a slight increase in cell size and in a substantial increase in intracellular pH. Taken together, our results suggest that the fluxes of glycerol in both directions across the plasma membrane exist in yeast cells simultaneously, and the export or import predominates according to the actual specific conditions.
authors: Department of Membrane Transport, Institute of Physiology Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 14220 Prague, Czech Republic.
authors: Michala
authors: M
authors: Duskova
authors: Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, LV-1586 Riga, Latvia.
authors: Diana
authors: D
authors: Borovikova
authors: Department of Membrane Transport, Institute of Physiology Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 14220 Prague, Czech Republic.
authors: Pavla
authors: P
authors: Herynkova
authors: Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, LV-1586 Riga, Latvia.
authors: Alexander
authors: A
authors: Rapoport
authors: Department of Membrane Transport, Institute of Physiology Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 14220 Prague, Czech Republic sychrova@biomed.cas.cz.
authors: Hana
authors: H
authors: Sychrova
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C470555:STL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D005990:Glycerol
keywords: Saccharomyces cerevisiae; desiccation; glycerol transport; osmotic stress