title: Thiamin analysis in red wine by fluorescence reverse phase-HPLC.
abstract: The derivatization of thiamin vitamers to their respective thiochrome by ferricyanide to facilitate fluorescence detection following separation by HPLC provides a powerful analytical tool. However the polyphenolic compounds in red wine readily interact with ferricyanide, reducing the effectiveness of ferricyanide oxidation in the derivatization of thiamin. We describe a method to facilitate the removal of polyphenolic compounds that interfere with the ferricyanide derivatization of thiamin. Polyvinylpolypyrrolidone afforded the total removal of phenolic compounds from red wines and allowed a spike recovery of thiamin vitamers (101% for thiamin; 104% for TMP; and 100% for TDP) in a wide range of red wines. This research found that Merlot styles of red wine contained the highest concentration of total thiamin (29.01 ng/mL) while Pinot Noir wines contained the lowest total concentration (8.27 ng/mL).
authors: Institute of Food and Crop Science, School of Health Sciences, Federation University Australia, Post Office Box 663, Ballarat 3353, Victoria, Australia.
authors: Callum
authors: C
authors: Liddicoat
authors: Institute of Food and Crop Science, School of Health Sciences, Federation University Australia, Post Office Box 663, Ballarat 3353, Victoria, Australia.
authors: Barry
authors: B
authors: Hucker
authors: Institute of Food and Crop Science, School of Health Sciences, Federation University Australia, Post Office Box 663, Ballarat 3353, Victoria, Australia; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors: Hao
authors: H
authors: Liang
authors: Institute of Food and Crop Science, School of Health Sciences, Federation University Australia, Post Office Box 663, Ballarat 3353, Victoria, Australia; Department of Food Science and Agri-Food Supply Chain Management, Harper Adams University, Newport, Shropshire TF10 8NB, United Kingdom. Electronic address: f.vriesekoop@harper-adams.ac.uk.
authors: Frank
authors: F
authors: Vriesekoop
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D013831:Thiamine / Q000031:analogs & derivatives* / Q000032:analysis; D014920:Wine / Q000032:analysis*
chemical_list: D013831:Thiamine
keywords: Ferricyanide; Polyphenols; Thiamin; Thiochrome; Vitamers