title: Involvement of orphan nuclear receptor COUP-TFII in cadherin-6 and cadherin-11 regulation: implications in development and cancer.
abstract: Changes in cadherin expression are instrumental both in embryonic development and disease, underlining the importance of understanding how cadherin expression is controlled. Kidney development is characterized by a mesenchymal-epithelial transition underlain by a cadherin-11 to cadherin-6 switch, the regulation mechanisms of which are presently unexplained. Using transfection and RNA-interference we demonstrate that COUP-TFII (NR2F2) induces down-regulation of cadherin-6 and up-regulation of cadherin-11 in cultured cell lines. Double immunolabeling of mouse embryos provides indirect evidence that COUP-TFII negatively controls the cadherin-11 to cadherin-6 switch underlying the kidney developmental mesenchymal-epithelial transition. Furthermore, we found high expression of COUP-TFII in some gastric and oesophageal adenocarcinomas, correlating with abnormal cadherin-11 expression and suggesting reactivation of embryonic pathways linked to COUP-TFII in these tumors. Altogether, our data shed new light upon the role of COUP-TFII in development and in cancer.
authors: Université Claude Bernard Lyon1, F-69622 Villeurbanne, France; INSERM, U865, IFR62, F-69 372 Lyon, France; Cancer Research Center of Lyon, UMR 1052, INSERM, 69 372, Lyon cedex 08, France. Electronic address: pierre.bringuier@univ-lyon1.fr.
authors: Pierre-Paul
authors: PP
authors: Bringuier
authors: Department of Urology, Nijmegen Medical Centre, Radboud University, Nijmegen, The Netherlands.
authors: Jack A
authors: JA
authors: Schalken
authors: Université Claude Bernard Lyon1, F-69622 Villeurbanne, France; Cancer Research Center of Lyon, UMR 1052, INSERM, 69 372, Lyon cedex 08, France.
authors: Valérie
authors: V
authors: Hervieu
authors: INSERM, U865, IFR62, F-69 372 Lyon, France. Electronic address: laurence.giroldi@inserm.fr.
authors: Laurence A
authors: LA
authors: Giroldi
mesh_terms: D000818:Animals; D051837:COUP Transcription Factor II / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D051837:COUP Transcription Factor II; D015820:Cadherins; C086960:osteoblast cadherin; C087310:K cadherin
keywords: Cadherins; Fibrosis; Gastric/oesophageal cancer; Implantation; Kidney mesenchymal–epithelial transition