title: Rad25 protein is targeted for degradation by the Ubc4-Ufd4 pathway.
abstract: Proteasome-mediated proteolysis provides dynamic spatial and temporal modulation of protein concentration in response to various intrinsic and extrinsic challenges. To gain a better understanding of the role of the proteasome in DNA repair, we systematically monitored the stability of 26 proteins involved in nucleotide excision repair (NER) under normal growth conditions. Among six NER factors found to be regulated by the proteasome, we further delineated the specific pathway involved in the degradation of Rad25, a subunit of TFIIH. We demonstrate that Rad25 turnover requires the ubiquitin-conjugating enzyme Ubc4 and the ubiquitin ligase Ufd4. Interestingly, the deletion of UFD4 specifically suppresses the rad25 mutant defective in transcription. Our results reveal a novel function of the Ufd4 pathway and another tie between the proteasome and NER regulators.
authors: From the Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 and baox@livemail.uthscsa.edu.
authors: Xin
authors: X
authors: Bao
authors: the Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844.
authors: Jill L
authors: JL
authors: Johnson
authors: From the Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 and raoh@uthscsa.edu.
authors: Hai
authors: H
authors: Rao
mesh_terms: D004265:DNA Helicases / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D051758:Transcription Factor TFIIH / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: C489575:SSL2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D051758:Transcription Factor TFIIH; C501306:Ubc4 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; C509339:Ufd4 protein, S cerevisiae; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex; D004265:DNA Helicases
keywords: DNA Repair; E3 Ubiquitin Ligase; Proteasome; Protein Degradation; Ubiquitin; Ubiquitylation (Ubiquitination)