title: Establishment of a DsRed-monomer-harboring ICR transgenic mouse model and effects of the transgene on tissue development.
abstract: DsRed-monomer is an enhanced red fluorescent protein that may serve as a marker for studies in biotechnology and cell biology. Since the ICR mouse strain is a widely utilized outbred strain for oncology, toxicology, vaccine development and for aging studies, the objective of this study was to produce a DsRed-monomer transgenic mouse by means of pronuclear micro-injection of a vector driven by the cytomegalovirus (CMV) enhancer/chicken beta-actin promoter. Four transgenic mice were successfully produced, one of which expressed the DsRed-monomer protein in every tissue, although at varying levels. High expression levels were observed in the heart, pancreas and muscle. Moreover, amniotic fluid-derived progenitor cells, which also expressed the DsRed-monomer protein, could be collected from the DsRed-monomer- harboring ICR mice. As compared to wild-type mice, a few biochemical and histological dissimilarities were found in the DsRed-monomer transgenic mice, including the presence of intra-cytoplasmic eosinophilic threadlike materials in the acinar cells. Taken together, transgenic mice stably expressing DsRed-monomer can be produced using pronuclear micro-injection; however, expression of the DsRed-monomer gene or its insertion position may lead to minor influences.
authors: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Chih-Jen
authors: CJ
authors: Chou
authors: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Shao-Yu
authors: SY
authors: Peng
authors: Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Cho-Hua
authors: CH
authors: Wan
authors: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Sou-Fu
authors: SF
authors: Chen
authors: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Winston Teng-Kui
authors: WT
authors: Cheng
authors: Department of Orthopaedics, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, Republic of China.
authors: Kun-Yi
authors: KY
authors: Lin
authors: Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors: Shinn-Chih
authors: SC
authors: Wu
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008845:Microinjections; D019076:Transgenes
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; C413663:fluorescent protein 583