title: Icariin Acts as a Potential Agent for Preventing Cardiac Ischemia/Reperfusion Injury.
abstract: Myocardial infarction is a leading cause of mortality and morbidity worldwide. Although essential for successful recovery, myocardium reperfusion is associated with reperfusion injury. Icariin, a major flavonoid of Epimedium koreanum Nakai, has been proven to exert efficacy for improving cardiovascular function. We investigated the molecular effect and signal pathway of icariin on cardiac ischemia/reperfusion injury. In an in vivo model of infarct in rats, icariin (10 mg/kg) significantly attenuated myocardial infarct size induced by ischemia/reperfusion (I/R). From the TUNEL assay, icariin reduced the apoptotic cell induced by I/R and decreased blood indicators of creatine kinase, ischemia-modified albumin, and lactate dehydrogenase. All this effect was antagonized by the PI3K inhibitor LY294002. Meanwhile, icariin activated the PI3K/Akt/eNOS pathway. The PI3K inhibitor LY294002 suppressed icariin-mediated protective effect. These results suggest that icariin protects against myocardial ischemia reperfusion injury in rats by activating the PI3K/Akt/eNOS-dependent signal pathways and may be a useful drug for angiogenic therapy.
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Minghe
authors: M
authors: Zhai
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Liang
authors: L
authors: He
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Xingai
authors: X
authors: Ju
authors: Department of Emergency Medicine, The People's Hospital of Liaoning Province, Shenyang, 110016, People's Republic of China.
authors: Ling
authors: L
authors: Shao
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Guang
authors: G
authors: Li
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Yunli
authors: Y
authors: Zhang
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China.
authors: Yanjiao
authors: Y
authors: Liu
authors: Department of Laboratory Medicine, The People's Hospital of Liaoning Province, 33 Wenyi Road, Shenyang, 110016, People's Republic of China. hmzhao163@163.com.
authors: Hongmei
authors: H
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; C056599:icariin
keywords: I/R; Icariin; Myocardial; PI3K/Akt/eNOS