title: Changes in lung surfactant proteins in rats with lipopolysaccharide-induced fever.
abstract: The study was designed to prove the hypothesis that lipopolysaccharide (LPS)-induced fever elicits the changes in surfactant specific proteins, potentially related to thermal tachypnea. In adult rats fever was induced by intraperitoneal administration of LPS at a dose 100 microg/kg of body weight; control group received saline. Respiratory parameters, arterial blood gases and pH and colonic body temperature (BT) were recorded. Five hours later, surfactant proteins (SP) A, B, C and D were evaluated in bronchoalveolar lavage fluid (BALF) and lung tissue (LT). LPS evoked monophasic thermic response (at 300 min 38.7+/-0.2 vs. 36.4+/-0.3 °C, P 0.05) and an increase in minute ventilation due to changes in breathing rate and tidal volume. LPS-instilled animals had higher levels of SP-A and SP-D in LT (P 0.05 and 0.01), and higher SP-D in BALF (P 0.01) than controls. SP-B increased in LT and SP-C in BALF of animals with LPS (both P 0.05 vs. controls). The changes in all surfactant specific proteins are present in LPS-induced fever. Alterations of proteins related to local immune mechanisms (SP-A, SP-D) are probably a part of general inflammatory response to pyrogen. Changes in proteins related to surface activity (SP-B and SP-C) might reflect the effort of the body to stabilize the lungs in thermal challenge.
authors: Department of Physiology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia. calkovska@jfmed.uniba.sk.
authors: M
authors: M
authors: Kolomaznik
authors: I
authors: I
authors: Zila
authors: J
authors: J
authors: Kopincova
authors: D
authors: D
authors: Mokra
authors: A
authors: A
authors: Calkovska
mesh_terms: D000450:Aldosterone / Q000097:blood; D000818:Animals; D001831:Body Temperature; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005334:Fever / Q000378:metabolism* / Q000503:physiopathology; D007958:Leukocyte Count; D008070:Lipopolysaccharides; D008297:Male; D037661:Pulmonary Surfactant-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D011897:Random Allocation; D017208:Rats, Wistar; D012119:Respiration; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D037661:Pulmonary Surfactant-Associated Proteins; D017392:Thiobarbituric Acid Reactive Substances; D000450:Aldosterone