title: Profiling of Proteins Regulated by Venlafaxine during Neural Differentiation of Human Cells.
abstract: OBJECTIVE
  Antidepressants are known to positively influence several factors in patients with depressive disorders, resulting in increased neurogenesis and subsequent relief of depressive disorders. To study the effects of venlafaxine during neural differentiation at the cellular level, we looked at its effect on protein expression and regulation mechanisms during neural differentiation.
  
  
  METHODS
  After exposing NCCIT cell-derived EBs to venlafaxine during differentiation (1 day and 7 days), changes in protein expression were analyzed by 2-DE and MALDI-TOF MS analysis. Gene levels of proteins regulated by venlafaxine were analyzed by real-time RT-PCR.
  
  
  RESULTS
  Treatment with venlafaxine decreased expression of prolyl 4-hydroxylase (P4HB), ubiquitin-conjugating enzyme E2K (HIP2) and plastin 3 (T-plastin), and up-regulated expression of growth factor beta-3 (TGF-β3), dihydropyrimidinase-like 3 (DPYSL3), and pyruvate kinase (PKM) after differentiation for 1 and 7 days. In cells exposed to venlafaxine, the mRNA expression patterns of HIP2 and PKM, which function as negative and positive regulators of differentiation and neuronal survival, respectively, were consistent with the observed changes in protein expression.
  
  
  CONCLUSIONS
  Our findings may contribute to improve understanding of molecular mechanism of venlafaxine.
authors: Department of Molecular and Life Sciences, Hanyang University, Ansan, Republic of Korea.
authors: Mi Sook
authors: MS
authors: Doh
authors: Department of Molecular and Life Sciences, Hanyang University, Ansan, Republic of Korea.
authors: Dal Mu Ri
authors: DM
authors: Han
authors: Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Republic of Korea.
authors: Dong Hoon
authors: DH
authors: Oh
authors: Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Republic of Korea.
authors: Seok Hyeon
authors: SH
authors: Kim
authors: Department of Molecular and Life Sciences, Hanyang University, Ansan, Republic of Korea.
authors: Mi Ran
authors: MR
authors: Choi
authors: Department of Molecular and Life Sciences, Hanyang University, Ansan, Republic of Korea.
authors: Young Gyu
authors: YG
authors: Chai
keywords: Depression; HIP2; NCCIT cells; Proteomics; Pyruvate kinase; Venlafaxine