title: Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage-like cell line J774.
abstract: WSXWS motif is a conserved amino acid sequence that is present in type I cytokine receptors. This motif that can be found both in the ligand binding chains and signal transducer molecule of the receptors with different amino acids at the position "X" plays a role in the receptor folding, ligand binding and signal transduction as well. Structural analysis proved that WSEWS motif of IL-6R is located in a highly accessible location in the protein. Structural properties and chemotaxis of a tetrapeptide library with SXWS sequence, where X was the 19 proteinogenic amino acids except cystein were systematically studied earlier. It has been proved that C-terminal amidation and the identity of amino acid X had a pronounced influence on the chemotactic properties but less of the structure of the peptides. Here, we present our findings on the effect of a tetrapeptide and a pentapeptide library with the sequence of SXWS and WSXWS on the chemotaxis and adhesion of J774 murine macrophage cell line. We studied the effect of the presence/absence of N-terminal tryptophan and the different amino acids at the X position on these physiological responses. Results indicated that amino acid X had a marked influence on chemotaxis, adhesion as well as on proliferation induced by (W)SXWS peptides. Elongation of SXWS sequence with a tryptophan at the N terminus also altered pronouncedly all the physiological responses of the cells studied. A good correlation could be observed between the chemotaxis and the proliferation and physicochemical parameters of the amino acid X.
authors: Research Group of Peptide Chemistry, Hungarian Academy of Sciences, P.O. Box 32, H-1518, Budapest, 112, Hungary.
authors: Rita
authors: R
authors: Szabó
authors: Orsolya
authors: O
authors: Láng
authors: Júlia
authors: J
authors: Láng
authors: Eszter
authors: E
authors: Illyés
authors: László
authors: L
authors: Kőhidai
authors: Ferenc
authors: F
authors: Hudecz
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002633:Chemotaxis; D050822:Cytokine Receptor gp130 / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000378:metabolism*
chemical_list: C494200:Il6st protein, mouse; D010455:Peptides; D050822:Cytokine Receptor gp130
keywords: IL-6R peptides; J774 cells; adhesion; chemotaxis; physicochemical parameters