title: Characterization of virus-like particles and identification of capsid proteins in Xanthophyllomyces dendrorhous.
abstract: Two dsRNAs of estimated lengths of 5 (L1) and 3.7 (L2) kpb are commonly found in strains of the basidiomycetous yeast Xanthophyllomyces dendrorhous, and the presence of virus-like particles (VLPs) have been described in some strains. Recently, two putative totiviruses (XdV-L1A and XdV-L1B) were identified from L1 dsRNA and one (XdV-L2) from L2 dsRNA in the strain UCD 67-385. In some strains, there are smaller dsRNAs (0.9-1.4 kb) that probable are satellite elements. In this work, the VLPs from several strains of X. dendrorhous, which differ in their dsRNAs content, were separated by sucrose gradient and characterized in relation to the dsRNAs and proteins that compose them. It was found that all types of dsRNAs were encapsidated into VLPs, supporting the hypothesis that the smaller dsRNAs are satellite molecules. A main protein of approx. 76 or 37 kDa composed the virions that only have the L1-dsRNA or L2-dsRNA, respectively. In the strain UCD 67-385, these both proteins were identified as viral capsid protein (CP), allow to confirm the gag predicted ORFs in XdV-L1A, XdV-L1B, and XdV-L2, with CPs of 76.6, 76.2, and 38.8 kDa, respectively. Analysis of predicted structures of CPs of XdV-L1A and XdV-L1B, showed high similitudes with the CPs of ScV-L-A and other totiviruses.
authors: Laboratorio de Genética, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile, oriana.flores@gmail.com.
authors: Oriana
authors: O
authors: Flores
authors: Jennifer
authors: J
authors: Alcaíno
authors: María
authors: M
authors: Fernandez-Lobato
authors: Víctor
authors: V
authors: Cifuentes
authors: Marcelo
authors: M
authors: Baeza
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D019197:Totivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014771:Virion / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D036022:Capsid Proteins