title: Putative novel lineage of West Nile virus in Uranotaenia unguiculata mosquito, Hungary.
abstract: West Nile virus (WNV) is an increasing public health concern in Europe with numerous human cases. A total of 23,029 female mosquitoes were tested for a variety of mosquito-borne flaviviruses and orthobunyaviruses supposedly endemic in Southern Transdanubia, Hungary, in the frames of a large-scale surveillance between 2011 and 2013. WNV nucleic acid was detected in a single pool containing Uranotaenia unguiculata mosquitoes. Sequence- and phylogenetic analyses for two different regions (NS5 and E) of the viral genome showed that the novel Hungarian WNV strain was different from other previously described WNV lineages. These findings may indicate the presence of a putative, novel lineage of WNV in Europe. Our results also indicate that U. unguiculata mosquito may become relevant species as a potential vector for West Nile virus in Europe.
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Gábor
authors: G
authors: Kemenesi
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Bianka
authors: B
authors: Dallos
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Miklós
authors: M
authors: Oldal
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Anna
authors: A
authors: Kutas
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Fanni
authors: F
authors: Földes
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Viktória
authors: V
authors: Németh
authors: Institut Pasteur, Paris, France.
authors: Paul
authors: P
authors: Reiter
authors: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary ; Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria.
authors: Tamás
authors: T
authors: Bakonyi
authors: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors: Krisztián
authors: K
authors: Bányai
authors: Virological Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság út 20, Pécs, 7624 Hungary ; Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors: Ferenc
authors: F
authors: Jakab
keywords: Hungary; Lineage; Uranotaenia unguiculata; West Nile virus