title: Bacteriocins and other bioactive substances of probiotic lactobacilli as biological weapons against Neisseria gonorrhoeae.
abstract: In the search of new antimicrobial agents against Neisseria gonorrhoeae, the bacteriocins-producing probiotic lactobacilli deserve special attention. The inhibitory effects of biosubstances such as organic acids, hydrogen peroxide and each bacteriocin-like inhibitory substance (BLIS) L23 and L60 on the growth of different gonococcal strains were investigated. Different non-treated and treated cell-free supernatants of two probiotic lactobacilli containing these metabolites were used. The aims of this work were (i) to evaluate the antimicrobial activity of the biosubstances produced by two probiotic lactobacilli, estimating the proportion in which each of them is responsible for the inhibitory effect, (ii) to define their minimum inhibitory concentrations (MICs) and, (iii) to determine the potential interactions between these biosubstances against N. gonorrhoeae. The main antimicrobial metabolites were the BLIS-es L23 and L60 in comparison with other biosubstances. Proportionally, their contributions to the inhibition on the gonococcal growth were 87.28% and 80.66%, respectively. The MIC values of bacteriocins were promising since these substances, when diluted, showed considerable inhibitory activity for all gonococci. In the interaction between bacteriocins, 100% of synergism was found on the gonococcal growth. In summary, this study indicates that both L23 and L60 could potentially serve to design new bioproducts against N. gonorrhoeae.
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina fruiz@exa.unrc.edu.ar.
authors: Francisco O
authors: FO
authors: Ruíz
authors: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina.
authors: Liliana
authors: L
authors: Pascual
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina.
authors: Walter
authors: W
authors: Giordano
authors: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina.
authors: Lucila
authors: L
authors: Barberis
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D000898:Antibiosis; D001430:Bacteriocins / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism; D004357:Drug Synergism; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000502:physiology*; D014621:Vagina / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D002264:Carboxylic Acids; D006861:Hydrogen Peroxide
keywords: Gram-negative diplococci; antagonistic activity; beneficial microorganisms; biostrategy; secondary metabolites; synergistic activity; therapeutic option