title: Pore architecture and cell viability on freeze dried 3D recombinant human collagen-peptide (RHC)-chitosan scaffolds.
abstract: Pore architecture of 3D scaffolds used in tissue engineering plays a critical role in the maintenance of cell survival, proliferation and further promotion of tissue regeneration. We investigated the pore size and structure, porosity, swelling as well as cell viability of a series of recombinant human collagen-peptide-chitosan (RHCC) scaffolds fabricated by lyophilization. In this paper, freezing regime containing a final temperature of freezing (Tf) and cooling rates was applied to obtain scaffolds with pore size ranging from 100μm to 120μm. Other protocols of RHC/chitosan suspension concentration and ratio modification were studied to produce more homogenous and appropriate structural scaffolds. The mean pore size decreased along with the decline of Tf at a slow cooling rate of 0.7°C/min; a more rapid cooling rate under 5°C/min resulted to a smaller pore size and more homogenous microstructure. High concentration could reduce pore size and lead to thick well of scaffold, while improved the ratio of RHC, lamellar and fiber structure coexisted with cellular pores. Human umbilical vein endothelial cells (HUVECs) were seeded on these manufactured scaffolds, the cell viability represented a negative correlation to the pore size. This study provides an alternative method to fabricate 3D RHC-chitosan scaffolds with appropriate pores for potential tissue engineering.
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Jing
authors: J
authors: Zhang
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Aimei
authors: A
authors: Zhou
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Aipeng
authors: A
authors: Deng
authors: Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors: Yang
authors: Y
authors: Yang
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Lihu
authors: L
authors: Gao
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Zhaocai
authors: Z
authors: Zhong
authors: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: yshulin@njust.edu.cn.
authors: Shulin
authors: S
authors: Yang
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry / Q000235:genetics*; D005612:Freeze Drying; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011994:Recombinant Proteins / Q000737:chemistry; D054457:Tissue Scaffolds
chemical_list: D011994:Recombinant Proteins; D003094:Collagen; D048271:Chitosan
keywords: 3D scaffold; Cell viability; Freezing regime; Pore architecture