title: Anti-inflammatory activities of cardamonin from Alpinia katsumadai through heme oxygenase-1 induction and inhibition of NF-κB and MAPK signaling pathway in the carrageenan-induced paw edema.
abstract: Cardamonin is a chalcone isolated from Alpinia katsumadai. This study is aimed to evaluate treatment of cardamonin decreased the paw edema at the 5th hour after λ-carrageenan (Carr) administration and increased the activities of catalase (CAT) and superoxide dismutase (SOD) in the anti-inflammatory test. We also demonstrated that cardamonin significantly attenuated the malondialdehyde (MDA) level in the edema paw at the 5th hour after Carr injection. Cardamonin decreased the nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 levels on the serum level at the 5th hour after Carr injection. Western blotting revealed that cardamonin decreased Carr-induced inducible nitric oxide synthase (iNOS), cycloxyclase (COX-2), nuclear factor-κB (NF-κB) and MAPK [extracellular signal-regulated protein kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), p38] expressions and increased heme oxygenase-1 (HO-1) expressions at the 5th hour in the edema paw. The anti-inflammatory mechanisms of cardamonin might be related to the decrease in the level of MDA, iNOS, COX-2, NF-κB, and MAPK and induction of the HO-1 expression in the edema paw via increasing the activities of CAT and SOD in the edema paw through the suppression of NO, TNF-α, IL-1β, and IL-6.
authors: School of Pharmacy, Zhejiang Medical College, Zhejiang, PR China.
authors: Yuan-Yuan
authors: YY
authors: Li
authors: School of Pharmacy, China Medical University, Taichung, Taiwan.
authors: Shyh-Shyun
authors: SS
authors: Huang
authors: Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors: Min-Min
authors: MM
authors: Lee
authors: Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan. Electronic address: dengjs@asia.edu.tw.
authors: Jeng-Shyan
authors: JS
authors: Deng
authors: School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan. Electronic address: gjhuang@mail.cmu.edu.tw.
authors: Guan-Jhong
authors: GJ
authors: Huang
mesh_terms: D032429:Alpinia; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D002351:Carrageenan; D002374:Catalase / Q000378:metabolism; D047188:Chalcones / Q000494:pharmacology* / Q000627:therapeutic use*; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism; D008813:Mice, Inbred ICR; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D047188:Chalcones; D016207:Cytokines; D008565:Membrane Proteins; D016328:NF-kappa B; D009569:Nitric Oxide; D002351:Carrageenan; D002374:Catalase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496512:Ptgs1 protein, mouse; D013482:Superoxide Dismutase; D020928:Mitogen-Activated Protein Kinases; C436747:cardamonin
keywords: Anti-inflammation; Cardamonin; Chinese medicine; HO-1; MAPK; NO