title: Evidence of feline panleukopenia infection in cats in India.
abstract: The samples collected from cats showing clinical signs suspected for feline panleukopenia infection were confirmed using various molecular techniques and virus isolation. The suspected samples were confirmed using feline parvovirus specific primers. The partial VP2 gene was submitted to GenBank for the first time in India (Accession number JQ684660.1). The PCR positive samples were further amplified using full length FPV VP2 gene specific primers and sequenced. The blast analysis revealed that the local field isolates of FPV showed 99 % homology with other FPV sequences available in the GenBank. The evidence for occurrence of feline panleukopenia infection in cats in Tamil Nadu, India was further confirmed by host specific nucleotides present in the VP2 gene region as well as virus isolation in A72 cell line.
authors: Department of Animal Biotechnology, Madras Veterinary College, Chennai, 600007 India.
authors: Manoharan
authors: M
authors: Parthiban
authors: Department of Animal Biotechnology, Madras Veterinary College, Chennai, 600007 India.
authors: Komandur Seshadri
authors: KS
authors: Aarthi
authors: Department of Animal Biotechnology, Madras Veterinary College, Chennai, 600007 India.
authors: Mani
authors: M
authors: Balagangatharathilagar
authors: Department of Animal Biotechnology, Madras Veterinary College, Chennai, 600007 India.
authors: Kathaperumal
authors: K
authors: Kumanan
keywords: Feline panleukopenia; Host specific nucleotides; Phylogenetic analysis; Polymerase chain reaction; VP2 gene