title: Physical properties and antifungal activity of bioactive films containing Wickerhamomyces anomalus killer yeast and their application for preservation of oranges and control of postharvest green mold caused by Penicillium digitatum.
abstract: This study assessed the ability of two bio-based films, obtained from sodium alginate (NaAlg) and locust bean gum (LBG), to protect the viability of Wickerhamomyces anomalus cells and control the growth of Penicillium digitatum. The effect of microbial cell incorporation on physical properties of the developed films was evaluated in terms of barrier, mechanical and optical properties. Furthermore, the application of these two matrices as bioactive coatings was investigated in order to evaluate their efficacy in preserving the postharvest quality of 'Valencia' oranges and inhibiting the growth of P. digitatum on artificially inoculated fruits. Results showed that NaAlg and LBG films were able to maintain more than 85% of the initial W. anomalus yeast population and that the developed films incorporating the killer yeast completely inhibited the growth of P. digitatum in synthetic medium. Likewise, NaAlg and LBG coatings enriched with W. anomalus yeast were effective at reducing weight loss and maintaining firmness of 'Valencia' oranges during storage, and reduced green mold in inoculated fruits by more than 73% after 13 days.
authors: Laboratoire des Substances Naturelles (LSN, LR10 INRAP02), Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Pôle Technologique de Sidi Thabet, 2020 Sidi Thabet, Tunisia.
authors: Hajer
authors: H
authors: Aloui
authors: Dipartimento di Agricoltura, Alimentazione e Ambiente (Di3A), University of Catania, Via Santa Sofia 98, 95123 Catania, Italy.
authors: Fabio
authors: F
authors: Licciardello
authors: Laboratoire des Substances Naturelles (LSN, LR10 INRAP02), Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Pôle Technologique de Sidi Thabet, 2020 Sidi Thabet, Tunisia. Electronic address: khaoula_khwaldia@yahoo.fr.
authors: Khaoula
authors: K
authors: Khwaldia
authors: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), 2 Boulevard de la Terre, BP 676, 1080 Tunis, Tunisia.
authors: Moktar
authors: M
authors: Hamdi
authors: Dipartimento di Agricoltura, Alimentazione e Ambiente (Di3A), University of Catania, Via Santa Sofia 98, 95123 Catania, Italy.
authors: Cristina
authors: C
authors: Restuccia
mesh_terms: D000464:Alginates / Q000378:metabolism; D000898:Antibiosis; D000935:Antifungal Agents / Q000592:standards*; D032084:Citrus sinensis / Q000382:microbiology*; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000382:microbiology*; D005685:Galactans / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development / Q000502:physiology*; D053149:Plant Gums / Q000378:metabolism; D004718:Saccharomycetales / Q000502:physiology*
chemical_list: D000464:Alginates; D000935:Antifungal Agents; D005685:Galactans; D006603:Hexuronic Acids; D008351:Mannans; D053149:Plant Gums; D020723:Glucuronic Acid; C017471:locust bean gum
keywords: Bioactive coating; Film properties; Locust bean gum; Postharvest quality; Sodium alginate; Wickerhamomyces anomalus