title: Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1.
abstract: Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.
authors: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917, C1033AAJ, Cuidad Autónoma de Buenos Aires, Argentina.
authors: Nicolás D
authors: ND
authors: Ayub
authors: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917, C1033AAJ, Cuidad Autónoma de Buenos Aires, Argentina.
authors: Ana R
authors: AR
authors: Fox
authors: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors: Araceli N
authors: AN
authors: García
authors: School of Biosciences and Biotechnology, University of Camerino, 62032 Camerino (MC), Italy.
authors: Matteo
authors: M
authors: Mozzicafreddo
authors: School of Biosciences and Biotechnology, University of Camerino, 62032 Camerino (MC), Italy.
authors: Massimiliano
authors: M
authors: Cuccioloni
authors: School of Biosciences and Biotechnology, University of Camerino, 62032 Camerino (MC), Italy.
authors: Mauro
authors: M
authors: Angeletti
authors: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
authors: Elba
authors: E
authors: Pagano
authors: Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917, C1033AAJ, Cuidad Autónoma de Buenos Aires, Argentina gabysoto80@gmail.com.
authors: Gabriela
authors: G
authors: Soto
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D019295:Computational Biology; D024881:Drug Resistance, Bacterial; D016680:Genome, Bacterial; D057166:High-Throughput Screening Assays; D000455:Medicago sativa / Q000737:chemistry; D062105:Molecular Docking Simulation; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D010641:Phenotype; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides
keywords: action mechanism; antimicrobial activity; antimicrobial peptides; innate immunity