title: Purification and characterization of an extracellular cold-adapted alkaline lipase produced by psychrotrophic bacterium Yersinia enterocolitica strain KM1.
abstract: An extracellular cold-adapted alkaline lipase from the psychrotrophic Yersinia enterocolitica strain KM1 was purified 26-fold to homogeneity. The enzyme was active over a broad range spanning 0-60 °C with an optimum activity at 37 °C, and it was found to be alkaline-preferring with an optimum activity at pH 9.0. The molecular weight was estimated to be 34.3 KDa and monomeric. The lipase could be activated by Ca(2+) and low concentration (10%) of ethanol, dimethyl sulphoxide, methanol, and acetonitrile, whereas it was strongly inhibited by Zn(2+), Cu(2+), SDS, EDTA, and PMSF. Using p-nitrophenyl butyrate as a substrate at 37 °C, the Km and Vmax of the enzyme were found to be 16.58 mM and 5.24 × 10(5)  μM · min(-1), respectively. This extracellular cold-adapted alkaline lipase may be a good candidate for detergents and biocatalysts at low temperature.
authors: Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Yunnan, China.|Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors: Xiuling
authors: X
authors: Ji
authors: Biochemistry and Molecule Biology, Department of Basic Medicine College, Dali University, Dali, China.
authors: Guiyuan
authors: G
authors: Chen
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors: Qi
authors: Q
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors: Lianbing
authors: L
authors: Lin
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors: Yunlin
authors: Y
authors: Wei
mesh_terms: D003080:Cold Temperature; D003902:Detergents / Q000494:pharmacology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013379:Substrate Specificity; D015008:Yersinia enterocolitica / Q000201:enzymology*
chemical_list: D003902:Detergents; D008049:Lipase
keywords: Characterization; Cold-adapted lipase; Purification; Yersinia enterocolitica