title: Gene silencing of Rac1 with RNA interference mediated by ultrasound and microbubbles in human LoVo cells: evaluation of cell invasion inhibition and metastatic.
abstract: OBJECTIVE
  The objective of this study was to assess the change of cytoskeleton and cell cycle in LoVo (human colorectal cancer) cell via gene silencing of Rac1 with RNA interference mediated by microbubble (SonoVue) and ultrasound (US).
  
  
  METHODS
  The compound of plasmid Rac1-shRNA, LoVo cells, and SonoVue was exposed to US (1 MHz, 2 W/cm(2), 5 min). The expression of Rac1 mRNA and Rac1 protein was detected by RT-PCR and Western blot. Cytoskeleton was taken by confocal microscope in a random fashion. Cell invasion was assayed using modified Boyden chambers, and cell cycle and apoptosis were analyzed by flow cytometry in LoVo cells.
  
  
  RESULTS
  Rac1 gene is overexpressed in human colorectal cancer cells, gene silencing of Rac1 with RNA interference mediated by microbubble and US strongly inhibited lamellipodia formation, cell invasion, and delayed cell cycle, as well as enhanced cell apoptosis of LoVo cells in vitro.
  
  
  CONCLUSIONS
  Silencing Rac1 gene mediated by microbubble and US may become a new treatment option for the inhibition of the invasion and metastasis of colorectal cancer cells.
authors: Department of Medical Ultrasonics, Lanzhou University Second Hospital , Lanzhou, Gansu , China and.
authors: Fang
authors: F
authors: Nie
authors: Xiao-Feng
authors: XF
authors: Wang
authors: Shi-Yi
authors: SY
authors: Zhao
authors: Lan
authors: L
authors: Bu
authors: Xue-Hui
authors: XH
authors: Liu
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D003599:Cytoskeleton / Q000235:genetics; D005434:Flow Cytometry; D020868:Gene Silencing; D006801:Humans; D045423:Microbubbles; D018613:Microscopy, Confocal; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000517:prevention & control; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014463:Ultrasonography / Q000379:methods; D020830:rac1 GTP-Binding Protein / Q000235:genetics*
chemical_list: D020830:rac1 GTP-Binding Protein
keywords: Actin cytoskeleton; RNA interference; Rac1; invasion colorectal cancer; microbubble and ultrasound