title: Fast and continuous-flow detection and separation of DNA complexes and DNA in nanofluidic chip format.
abstract: Fast separation of DNA and detection of protein/DNA complexes are important in many state-of-the-art molecular medicine technologies, like the production of gene vaccines or medical diagnostics. Here, we describe a nanofluidic chip-based technique for fast, efficient, and virtually label-free detection and separation of protein/DNA and drug/DNA complexes and topological DNA variants. The mechanism is based on a continuous-flow dielectrophoresis at a nanoslit and allows efficient separation of small DNA fragments (<7,000 base pairs) and fast detection of DNA complexes within 1 min.
authors: Experimental Biophysics and Applied Nanoscience, Faculty of Physics, Bielefeld University, 33615, Bielefeld, Germany, viefhues@physik.uni-bielefeld.de.
authors: Martina
authors: M
authors: Viefhues
authors: Jan
authors: J
authors: Regtmeier
authors: Dario
authors: D
authors: Anselmetti
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D004247:DNA