title: Identification and morphologic and molecular characterization of Cyclospora macacae n. sp. from rhesus monkeys in China.
abstract: Cyclospora spp. in nonhuman primates are most closely related to Cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in North America. Studies thus far indicate the possible existence of host specificity in Cyclospora spp. In this study, 411 fecal specimens from free-range rhesus monkeys (Macaca mulatta) were collected and examined for Cyclospora by sequence analysis of the small subunit rRNA gene. A novel Cyclospora species was identified in 28 (6.8%) specimens and named Cyclospora macacae based on morphologic and molecular characterizations. The oocyst of C. macacae is spherical and measures 8.49 ± 0.55 × 8.49 ± 0.49 μm in diameter. Phylogenetic analysis grouped this species together with the other four Cyclospora species infecting primates, including C. cayetanensis in humans, forming a monophyletic group closely related to avian Eimeria species. In addition, C. cayetanensis was detected in one specimen, although whether rhesus monkeys can serve as a natural reservoir host of C. cayetanensis needs further investigation.
authors: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China.
authors: Na
authors: N
authors: Li
authors: Jianbin
authors: J
authors: Ye
authors: Michael J
authors: MJ
authors: Arrowood
authors: Jingbo
authors: J
authors: Ma
authors: Lin
authors: L
authors: Wang
authors: Hailing
authors: H
authors: Xu
authors: Yaoyu
authors: Y
authors: Feng
authors: Lihua
authors: L
authors: Xiao
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D021744:Cyclospora / Q000302:isolation & purification*; D021866:Cyclosporiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D006801:Humans; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000453:epidemiology / Q000469:parasitology*; D009656:North America; D010802:Phylogeny; D016133:Polymerase Chain Reaction