title: Proliferation and decidualization of endometrial stromal cells during embryo-attachment stage in bonnet monkeys (Macaca radiata).
abstract: We report embryo-induced alterations occurring in endometrial stromal cells (ESCs) during the embryo-attachment stage in bonnet monkeys (Macaca radiata). Laser micro-dissected ESCs obtained from pregnant and non-pregnant animals were compared for levels of selected proliferation and decidualization-associated factors by analysis with quantitative real-time polymerase chain reaction or immunohistochemistry. Stromal cells exhibited extensive cellular proliferation, as indicated by cellular compaction and significantly higher (P < 0.05) levels of proliferating cell nuclear antigen and of estrogen receptor 1, c-Myc, and Cyclin D1 transcripts in pregnant animals as compared with non-pregnant animals. A significant decrease (P < 0.05) was observed in the transcript levels of stromal interleukin-6 (IL-6) in pregnant animals. Cell proliferation was accompanied by a significant increase (P < 0.001) in the levels of decidualization-associated molecules such as IL-1β in the luminal and glandular epithelium and of stromal insulin-like growth-factor-binding protein-1 (IGFBP-1) and prostaglandin-endoperoxide synthase-2 (PTGS-2) proteins. In pregnant animals, proliferation was evident throughout the gestational stroma, whereas decidualization was more pronounced in the embryo-attachment zone than in the non-attachment zone. To our knowledge, this is the first report of alterations in the endometrial stroma during the embryo-attachment stage in a non-human primate model.
authors: Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, JM Street, Parel, Mumbai, 400012, India.
authors: Shruti
authors: S
authors: Nimbkar-Joshi
authors: Rajendraprasad R
authors: RR
authors: Katkam
authors: Ruchi
authors: R
authors: Kakar
authors: Priyanka
authors: P
authors: Singh
authors: Uddhav K
authors: UK
authors: Chaudhari
authors: Dhananjay D
authors: DD
authors: Manjramkar
authors: Siddhanath M
authors: SM
authors: Metkari
authors: Sanjeeva D
authors: SD
authors: Kholkute
authors: Chander P
authors: CP
authors: Puri
authors: Geetanjali
authors: G
authors: Sachdeva
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000032:analysis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000032:analysis / Q000235:genetics; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000235:genetics; D047629:Estrogen Receptor beta / Q000032:analysis / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis / Q000235:genetics; D053583:Interleukin-1beta / Q000032:analysis / Q000235:genetics; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics; D008255:Macaca radiata / Q000196:embryology* / Q000235:genetics; D011247:Pregnancy; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D018970:Insulin-Like Growth Factor Binding Protein 1; D053583:Interleukin-1beta; D015850:Interleukin-6; D016271:Proto-Oncogene Proteins c-myc; D019938:Cyclin D1; D051546:Cyclooxygenase 2