title: Adipose-derived mesenchymal stem cells inhibit activation of hepatic stellate cells in vitro and ameliorate rat liver fibrosis in vivo.
abstract: OBJECTIVE
  Previous studies suggested that mesenchymal stem cells may ameliorate fibrogenesis through the inhibition of hepatic stellate cells (HSCs) activation. This study aimed to investigate whether adipose derived mesenchymal stem cells (ADSCs) could modulate the activation of HSCs and contribute to the recovery of liver fibrogenesis.
  
  
  METHODS
  ADSCs and HSCs were isolated from Sprague-Dawley rats and co-cultured using a transwells insert. Cell proliferation, apoptosis and smooth muscle α-actin (α-SMA) expression in HSCs were examined. Rats were injected with CCl4 to induce liver fibrogenesis. After injection of ADSCs through portal vein, the rats were examined for pathological changes in the liver. α-SMA expression and hydroxyproline content in the liver and serum levels of collagen III and hyaluronic acid was detected.
  
  
  RESULTS
  After co-culturing for 72 h, the proliferation and activation of HSCs was inhibited by ADSCs and the apoptosis of HSCs was promoted by ADSCs. Transplantation of ADSCs inhibited liver fibrogenesis in the rats.
  
  
  CONCLUSIONS
  ADSCs inhibit the proliferation and activation of HSCs in vitro and inhibit liver fibrogenesis in rat model, suggesting the potential application of ADSCs in liver fibrogenesis therapy.
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Fuxiang
authors: F
authors: Yu
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Shiqiang
authors: S
authors: Ji
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Longfeng
authors: L
authors: Su
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Li
authors: L
authors: Wan
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Shengchu
authors: S
authors: Zhang
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Chunlei
authors: C
authors: Dai
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Yang
authors: Y
authors: Wang
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China.
authors: Junhui
authors: J
authors: Fu
authors: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, People's Republic of China. Electronic address: zqy80@163.com.
authors: Qiyu
authors: Q
authors: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D017209:Apoptosis; D002251:Carbon Tetrachloride; D049109:Cell Proliferation; D002478:Cells, Cultured; D055166:Hepatic Stellate Cells / Q000166:cytology*; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000199:Actins; D002251:Carbon Tetrachloride
keywords: adipose derived mesenchymal stem cells; fibrosis; hepatic stellate cells; proliferation