title: Combination of miR-21 with Circulating Tumor Cells Markers Improve Diagnostic Specificity of Metastatic Breast Cancer.
abstract: Circulating miR-21 is upregulated in breast cancer. However, correlation of miR-21 expression with clinic pathologic characteristics remains questionable. In this study, we investigate whether combination of circulation miR-21 with circulating tumor cells (CTCs) marker (EpCAM, MUS1, HER2) could improve diagnostic specificity of metastatic breast cancer. Total 223 breast cancer patients were included. 89 % patients were associated with upregulation of miR-21 compared with health control. 20 % patients were detected for CTCs marker positive. For higher specificity purpose, triple marker positive samples were selected as true CTCs positive, which only occupied 59.5 % of total metastatic breast cancer patients. Specificity of detection of CTCs was 96.7 %. Furthermore, 59.5 % metastatic breast cancer patients were shown both abnormal miR-21 and true CTCs positive according to distribution of true CTCs positive and abnormal miR-21; Combination of miR-21 and CTCs was increased specificity of metastatic detection to 100 %. Our findings suggested that combination of miR-21 with CTCs marker could be used for better diagnosis of metastatic breast cancer in the future.
authors: Departments of Surgery, People's Hospital of Linzi District, Affiliated to Binzhou Medical College, Zibo, 255400, China.
authors: Xingwang
authors: X
authors: Yang
authors: Departments of Surgery, People's Hospital of Linzi District, Affiliated to Binzhou Medical College, Zibo, 255400, China.
authors: Xiaoming
authors: X
authors: Wang
authors: Department of Thyroid and Breast Surgery, Linyi People's Hospital, Linyi, 276003, Shandong, China.
authors: Hongyan
authors: H
authors: Shen
authors: Lunan Pharmaceutical Group Co., Ltd., Linyi, 276005, Shandong, China.
authors: Rong
authors: R
authors: Deng
authors: Department of Thyroid and Breast Surgery, Linyi People's Hospital, Linyi, 276003, Shandong, China. xuekecheng@yeah.net.
authors: Kecheng
authors: K
authors: Xue
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D000071858:Epithelial Cell Adhesion Molecule / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D018396:Mucin-1 / Q000097:blood; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D035683:MicroRNAs; D018396:Mucin-1; D018719:Erb-b2 Receptor Tyrosine Kinases; C499379:EPCAM protein, human; C524940:MIRN21 microRNA, human; C060192:MUC1 protein, human; C508053:ERBB2 protein, human
keywords: Circulating tumor cells; Diagnosis; Metastasis breast cancer; Specificity; miR-21