title: LKB1 suppresses proliferation and invasion of prostate cancer through hedgehog signaling pathway.
abstract: Activation of the hedgehog (Hh) signaling pathway has been implicated in the development of many human malignancies. Hh signaling target genes, such as patched (PTCH), smoothened (SMO) and sonic hedgehog (SHH), are markers of Hh signaling activation in most Hh-associated tumors. The protein kinase LKB1 has been shown to slow proliferation and induce cell-cycle arrest in many cell lines. However, the function of LKB1 in prostate cancer development remains largely unclear. In this study, the expression of LKB1 in human prostate cancer tissue samples and prostate cancer cell lines was detected, and the effects of LKB1 on prostate cancer cell proliferation and invasion were evaluated. Moreover, the influence of LKB1 on target genes of the Hh signaling pathway was analyzed. The results indicated that knockdown of LKB1 expression by RNA interference promoted cell proliferation, colony formation and invasion. Meanwhile, we observed that LKB1 siRNA increased the expression of factors related to Hh signaling reporter activity in prostate cancer cells, including PTCH, SMO and SHH. These findings suggest that LKB1 is a putative tumor suppressor gene in prostate cancer, and that LKB1 is negatively correlated with the expression of Hh signaling related transcription factors. Our results suggest that LKB1 may inhibit tumorigenesis by regulating the Hh signaling pathway in certain cancers.
authors: Department of Urology, First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, PR China.
authors: Peiyuan
authors: P
authors: Xu
authors: Department of Urology, First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, PR China.
authors: Fei
authors: F
authors: Cai
authors: Department of Urology, First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, PR China.
authors: Xiaofei
authors: X
authors: Liu
authors: Department of Urology, First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, PR China.
authors: Lele
authors: L
authors: Guo
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology
chemical_list: D053823:Hedgehog Proteins; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases
keywords: LKB1; Prostate cancer; hedgehog (Hh) signaling pathway; invasion; proliferation