title: Simultaneous quantitative determination of 20 active components in the traditional Chinese medicine formula Zhi-Zi-Da-Huang decoction by liquid chromatography coupled with mass spectrometry: application to study the chemical composition variations in different combinations.
abstract: Zhi-Zi-Da-Huang decoction (ZZDHD), a classical traditional Chinese medicine (TCM) prescription composed of four herbal medicines, has been widely used in treating various hepatobiliary disorders for a long time. The objective of this study was to develop a sensitive and efficient liquid chromatography coupled with mass spectrometry (LC-MS) method for quantitative determination of 20 active constituents, including three iridoid glycosides, 11 flavonoids, three anthraquinones and three tannins in ZZDHD. Separation was achieved on a phenomenex kinetex C18 column (150 × 4.6 mm, 2.6 µm) using gradient elution with a mobile phase consisting of acetonitrile and 0.1% formic acid in water. Detection was performed with electrospray ionization source in the negative ionization and selected ion monitoring mode. The established method was validated by determining the linearity (r(2) ≥ 0.9983), limit of quantification (0.16-300 ng/mL), precision (RSD ≤ 4.6%), average recovery (96.0-105.6%), repeatability (RSD ≤ 3.2%) and stability (RSD ≤ 4.5%). Then, the method was successfully applied to investigate the chemical composition variations owing to the interaction between the four component herbs of ZZDHD during the extraction process. It was found that different combinations of the herbs affect the extraction efficiency of chemical constituents in different ways. The validated LC-MS method provides a meaningful basis for quality control and further research on ZZDHD.
authors: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Zheng
authors: Z
authors: Tang
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Ran
authors: R
authors: Yin
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Kaishun
authors: K
authors: Bi
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.|School of Pharmacy, Jilin Medical College, Jilin, 132001, China.
authors: Heyun
authors: H
authors: Zhu
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Fei
authors: F
authors: Han
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Kelin
authors: K
authors: Chen
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors: Fenrong
authors: F
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Zhi-Zi-Da-Huang decoction; compatibility; liquid chromatography coupled with mass spectrometry; simultaneous quantification