title: Platelet-rich plasma, especially when combined with a TGF-β inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
abstract: Regeneration of skeletal muscle after injury is limited by scar formation, slow healing time and a high recurrence rate. A therapy based on platelet-rich plasma (PRP) has become a promising lead for tendon and ligament injuries in recent years, however concerns have been raised that PRP-derived TGF-β could contribute to fibrotic remodelling in skeletal muscle after injury. Due to the lack of scientific grounds for a PRP -based muscle regeneration therapy, we have designed a study using human myogenic progenitors and evaluated the potential of PRP alone and in combination with decorin (a TGF-β inhibitor), to alter myoblast proliferation, metabolic activity, cytokine profile and expression of myogenic regulatory factors (MRFs). Advanced imaging multicolor single-cell analysis enabled us to create a valuable picture on the ratio of quiescent, activated and terminally committed myoblasts in treated versus control cell populations. Finally high-resolution confocal microscopy validated the potential of PRP and decorin to stimulate the formation of polynucleated myotubules. PRP was shown to down-regulate fibrotic cytokines, increase cell viability and proliferation, enhance the expression of MRFs, and contribute to a significant myogenic shift during differentiation. When combined with decorin further synergistc effects were identified. These results suggest that PRP could not only prevent fibrosis but could also stimulate muscle commitment, especially when combined with a TGF-β inhibitor.
authors: Department of Orthopaedic Surgery, University Medical Center Maribor, Maribor, Slovenia.
authors: Robi
authors: R
authors: Kelc
authors: Institute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors: Martin
authors: M
authors: Trapecar
authors: Institute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors: Lidija
authors: L
authors: Gradisnik
authors: Institute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors: Marjan Slak
authors: MS
authors: Rupnik
authors: Department of Orthopaedic Surgery, University Medical Center Maribor, Maribor, Slovenia.
authors: Matjaz
authors: M
authors: Vogrin
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D058575:Decorin / Q000378:metabolism / Q000494:pharmacology; D003893:Desmin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D055435:Myostatin / Q000235:genetics / Q000378:metabolism; D053657:Platelet-Rich Plasma; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*
chemical_list: D058575:Decorin; D003893:Desmin; D055435:Myostatin; D016212:Transforming Growth Factor beta