title: ERCC1, RRM1 and TUBB3 mRNA expression on the tumor response and overall survival of non-small cell lung cancer treated with platinum-based chemotherapy.
abstract: OBJECTIVE
  We aimed to analyze the expression of ERCC1, RRM1 and TUBB3 in 305 patients with advanced non-small cell lung cancer (NSCLC) and investigate whether these genes can be used as biomarkers for predicting tumor response and clinical outcome.
  
  
  METHODS
  Total 305 patients with unresectable and locally advanced NSCLC were collected between January 2007 and December 2008. cDNA of ERCC1, RRM1 and TUBB3 was isolated by a fluorescence-based real-time detection method.
  
  
  RESULTS
  All the patients were followed up until December 2012. One hundred seventy five patients showed good response and 130 patients showed poor response to chemotherapy. 126 patients died and 166 patients showed progressive disease during the follow-up period. The median levels of ERCC1, RRM1 and TUBB3 mRNA were 0.53±0.13, 0.31±0.15 and 0.18±0.16, respectively. We found that patients with low ERCC1 expression showed a significantly higher rate of good tumor response, and the adjusted OR (95% CI) was 2.16(1.32-3.45). By Cox regression analysis. We also found that low ERCC1 expression level were correlated with longer overall survival of NSCLC patients, with the adjusted HR (95% CI) was 2.15 (1.26-3.35).
  
  
  CONCLUSIONS
  This study showed that ERCC1 mRNA expression can not affect the response to chemotherapy and clinical outcome of advanced non-small cell lung cancer (NSCLC) patients.
authors: Hongwei Qiao, The Second Respiratory Medicine Department, Xinxiang Central Hospital, Xinxiang, China.
authors: Hongwei
authors: H
authors: Qiao
authors: Xiaoping Huang, The Second Respiratory Medicine Department, Xinxiang Central Hospital, Xinxiang, China.
authors: Xiaoping
authors: X
authors: Huang
authors: Hua Guo, The Second Oncology Department, Xinxiang Central Hospital, Xinxiang, China.
authors: Hua
authors: H
authors: Guo
authors: Yan Liu, Department of Tumor Surgery, Xinxiang Central Hospital, Xinxiang, China.
authors: Yan
authors: Y
authors: Liu
authors: Chunyan Yue, The Second Oncology Department, Xinxiang Central Hospital, Xinxiang, China.
authors: Chunyan
authors: C
authors: Yue
keywords: ERCC1; Overall survival; RRM1; TUBB3; mRNA; Tumor response; non-small cell lung cancer