title: Lysozyme depolymerization of photo-activated chitosan adhesive films.
abstract: Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.
authors: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors: Damia
authors: D
authors: Mawad
authors: School of Biological Sciences, The University of Sydney, Sydney 2006, NSW, Australia.
authors: Charles
authors: C
authors: Warren
authors: School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia.
authors: Mathew
authors: M
authors: Barton
authors: School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia.
authors: David
authors: D
authors: Mahns
authors: School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia.
authors: John
authors: J
authors: Morley
authors: Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, Sydney 2006, NSW, Australia.
authors: Binh T T
authors: BT
authors: Pham
authors: Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, Sydney 2006, NSW, Australia.
authors: Nguyen T H
authors: NT
authors: Pham
authors: School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia.
authors: Sindy
authors: S
authors: Kueh
authors: School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia; Biomedical Engineering & Neuroscience (BENS) Research Group, The MARCS Institute, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia. Electronic address: a.lauto@uws.edu.au.
authors: Antonio
authors: A
authors: Lauto
mesh_terms: D000269:Adhesives / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D007834:Lasers; D009113:Muramidase / Q000378:metabolism*; D058105:Polymerization; D051381:Rats; D012395:Rose Bengal / Q000737:chemistry
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D012395:Rose Bengal; D048271:Chitosan; D009113:Muramidase
keywords: Capillary electrophoresis; Laser; Lysozyme; Mass spectroscopy