title: The evolution of phylogeographic data sets.
abstract: Empirical phylogeographic studies have progressively sampled greater numbers of loci over time, in part motivated by theoretical papers showing that estimates of key demographic parameters improve as the number of loci increases. Recently, next-generation sequencing has been applied to questions about organismal history, with the promise of revolutionizing the field. However, no systematic assessment of how phylogeographic data sets have changed over time with respect to overall size and information content has been performed. Here, we quantify the changing nature of these genetic data sets over the past 20 years, focusing on papers published in Molecular Ecology. We found that the number of independent loci, the total number of alleles sampled and the total number of single nucleotide polymorphisms (SNPs) per data set has improved over time, with particularly dramatic increases within the past 5 years. Interestingly, uniparentally inherited organellar markers (e.g. animal mitochondrial and plant chloroplast DNA) continue to represent an important component of phylogeographic data. Single-species studies (cf. comparative studies) that focus on vertebrates (particularly fish and to some extent, birds) represent the gold standard of phylogeographic data collection. Based on the current trajectory seen in our survey data, forecast modelling indicates that the median number of SNPs per data set for studies published by the end of the year 2016 may approach ~20,000. This survey provides baseline information for understanding the evolution of phylogeographic data sets and underscores the fact that development of analytical methods for handling very large genetic data sets will be critical for facilitating growth of the field.
authors: Department of Biology, University of Mississippi, Oxford, MS, 38677, USA.
authors: Ryan C
authors: RC
authors: Garrick
authors: Isabel A S
authors: IA
authors: Bonatelli
authors: Chaz
authors: C
authors: Hyseni
authors: Ariadna
authors: A
authors: Morales
authors: Tara A
authors: TA
authors: Pelletier
authors: Manolo F
authors: MF
authors: Perez
authors: Edwin
authors: E
authors: Rice
authors: Jordan D
authors: JD
authors: Satler
authors: Rebecca E
authors: RE
authors: Symula
authors: Maria Tereza C
authors: MT
authors: Thomé
authors: Bryan C
authors: BC
authors: Carstens
mesh_terms: D000818:Animals; D005260:Female; D051456:Gene Flow; D008297:Male; D010802:Phylogeny; D001503:Ursidae / Q000235:genetics*
keywords: DNA sequences; information content; phylogeography; sampling; single nucleotide polymorphisms; temporal trends