title: A choline oxidase amperometric bioassay for the detection of mustard agents based on screen-printed electrodes modified with Prussian Blue nanoparticles.
abstract: In this work a novel bioassay for mustard agent detection was proposed. The bioassay is based on the capability of these compounds to inhibit the enzyme choline oxidase. The enzymatic activity, which is correlated to the mustard agents, was electrochemically monitored measuring the enzymatic product, hydrogen peroxide, by means of a screen-printed electrode modified with Prussian Blue nanoparticles. Prussian Blue nanoparticles are able to electrocatalyse the hydrogen peroxide concentration reduction at low applied potential (-50 mV vs. Ag/AgCl), thus allowing the detection of the mustard agents with no electrochemical interferences. The suitability of this novel bioassay was tested with the nitrogen mustard simulant bis(2-chloroethyl)amine and the sulfur mustard simulants 2-chloroethyl ethyl sulfide and 2-chloroethyl phenyl sulfide. The bioassay proposed in this work allowed the detection of mustard agent simulants with good sensitivity and fast response, which are excellent premises for the development of a miniaturised sensor well suited for an alarm system in case of terrorist attacks.
authors: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy. fabiana.arduini@uniroma2.it.
authors: Fabiana
authors: F
authors: Arduini
authors: Dipartimento Agroalimentare, IC-CNR Istituto di Cristallografia, Via Salaria Km 29.3, 00015 Monterotondo Scalo, Rome 00015, Italy. viviana.scognamiglio@mlib.ic.cnr.it.
authors: Viviana
authors: V
authors: Scognamiglio
authors: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy. corrado.covaia@libero.it.
authors: Corrado
authors: C
authors: Covaia
authors: Laboratoire Génie des Procédés et Environnement, Faculté de Sciences et Techniques, Université Hassan II-Mohammedia, B.P.146, Mohammadia 20850, Morocco. azizamine@yahoo.fr.
authors: Aziz
authors: A
authors: Amine
authors: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy. moscone@uniroma2.it.
authors: Danila
authors: D
authors: Moscone
authors: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy. palleschi@uniroma2.it.
authors: Giuseppe
authors: G
authors: Palleschi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D005295:Ferrocyanides; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; C000170:ferric ferrocyanide