title: Lower esophageal microbiota species are affected by the eradication ofHelicobacter pyloriinfection using antibiotics.
abstract: The aim of this study was to investigate the effect ofHelicobacter pylori(H. pylori) infection on the lower esophageal microbiota and the eradication ofH. pylorithrough the use of antibiotics. Forty-five BALB/C mice were randomly divided into negative control, infection and treatment groups. The mice were sacrificed and DNA was extracted from the lower esophageal microbiota. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was performed to determine the composition of the microbiota. Quantity One®1-D Analysis Software was used for the analysis of the DGGE profiles. The different bands from the groups were amplified with 16S rDNA V6 region primers. DNA sequencing and Basic Local Alignment Search Tool analysis were performed for the identification of the bands.H. pyloricolonization led to severe ulcers in the stomachs of the mice, and these ulcers were alleviated by antibiotic treatment. The infection group had an increased number of bacterial species in the stomach compared with the control and treatment groups. DGGE fingerprinting of the lower esophagus showed that there were significant differences in the number of bands (P<0.05), diversity index and abundance among the groups (P<0.05); however, no significant differences in homogeneity were observed (P>0.05). Although the composition of flora species in the lower espohagus varied, the dominant species, and their relative contents, were similar in the control, infection and treatment groups. The present study provided a microecological basis for the understanding of the pathogenesis of lower esophageal diseases, following the eradication ofH. pyloriinfection with antibiotics.
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Zhiying
authors: Z
authors: Tian
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China ; Laboratory of Pathogen Biology and Immunology, Teaching and Experiment Center of Basic Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Zhibang
authors: Z
authors: Yang
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Jiye
authors: J
authors: Gao
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Lili
authors: L
authors: Zhu
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Renju
authors: R
authors: Jiang
authors: Department of Pathogen Biology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Ying
authors: Y
authors: Jiang
keywords: Helicobacter pylori; antibiotics; infection; lower esophagus; microbiota; polymerase chain reaction-denaturing gradient gel electrophoresis