title: Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.
abstract: We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morphology and substrate biochemistry regulate tissue morphogenesis.
authors: Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA. rashton2@wisc.edu.
authors: G T
authors: GT
authors: Knight
authors: J
authors: J
authors: Sha
authors: R S
authors: RS
authors: Ashton
mesh_terms: D018929:Cell Culture Techniques; D057930:Click Chemistry; D006801:Humans; D015394:Molecular Structure; D009417:Nerve Tissue / Q000166:cytology*; D010455:Peptides / Q000737:chemistry*; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D010455:Peptides