title: Stimulatory effect of sesamin on hepatic cytochrome P450 activities in Atlantic salmon (Salmo salar L.) is not directly associated with expression of genes related to xenobiotic metabolism.
abstract: 1. This study examined hepatic cytochrome P450 (CYP450) response to dietary sesamin in combination with different n-6/n-3 fatty acid ratios in fish diet. Over a period of 4 months, fish were fed seven different experimental diets an n-6/n-3 FA ratio of either 0.5 or 1.0 in combination with two sesamin levels: low sesamin = 1.16 g/kg feed and high sesamin = 5.8 g/kg feed. Control diets did not contain sesamin. 2. The CYP450-associated activities of ethoxyresorufin O-deethylase (EROD), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD), pentoxyresorufin O-depentylase (PROD), coumarin hydroxylase (COH), methoxyresorufin O-deethylase (MROD) and p-nitrophenol hydroxylase (PNPH) were significantly induced by dietary sesamin in a dose-related manner. 3. Expressions of the genes CYP1A1, CYP1A3, CYP3A, AhR1α, AhR2β, AhR2δ and PXR involved in the regulation of CYP450 activities, was not the primary source of this induction.
authors: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice , Vodnany , Czech Republic and.
authors: Vladimir
authors: V
authors: Zlabek
authors: AnnaLotta Schiller
authors: AS
authors: Vestergren
authors: Sofia
authors: S
authors: Trattner
authors: Liane
authors: L
authors: Wagner
authors: Jana
authors: J
authors: Pickova
authors: Galia
authors: G
authors: Zamaratskaia
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004149:Dioxoles / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D017705:Lignans / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010938:Plant Oils / Q000494:pharmacology; D020317:Salmo salar / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D004149:Dioxoles; D005395:Fish Oils; D017705:Lignans; D010938:Plant Oils; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; C054125:sesamin
keywords: Fish; induction; xenobiotic metabolizing enzymes