title: Clic1 plays a role in mouse hepatocarcinoma via modulating Annexin A7 and Gelsolin in vitro and in vivo.
abstract: Clic1 is a member of the family of chloride intracellular ion channels. Previous studies suggest that Clic1 is involved in migration and invasion of the lymphatic metastasis in hepatocarcinoma, however, the mechanism is not fully understood. In the present study, we observed Clic1 is abundant in cytoplasm, higher expression in Hca-F cell than Hca-P cell, and we showed that downregulation of Clic1 by RNA interference was able to markedly enhance the expression of tumor metastasis genes Annexin A7 and Gelsolin in vitro, and downregulation of Annexin A7 and Gelsolin also enhanced the expression of Clic1 in vitro and in vivo. Our results provide novel insight that Clic1 have a role in migration and invasion in hepatocarcinoma maybe via modulating the expression of Annexin A7 and Gelsolin, and provide novel insight into the mechanisms of Clic1 for hepatocarcinoma treatment.
authors: Department of Pathology, Dalian Medical University, Dalian, China. Electronic address: zhangjun@dlmedu.edu.cn.
authors: Jun
authors: J
authors: Zhang
authors: Department of Pathology, Dalian Medical University, Dalian, China.
authors: Miaoling
authors: M
authors: Li
authors: Department of Pathology, Dalian Medical University, Dalian, China.
authors: Meiying
authors: M
authors: Song
authors: Department of Pathology, Dalian Medical University, Dalian, China.
authors: Wenjing
authors: W
authors: Chen
authors: Department of Pathology, Dalian Medical University, Dalian, China.
authors: Jun
authors: J
authors: Mao
authors: Department of Pathology, Dalian Medical University, Dalian, China.
authors: Lin
authors: L
authors: Song
authors: Department of Pathology, Dalian Medical University, Dalian, China; The Key Laboratory of Tumor Metastasis in Liaoning Province, Dalian, Liaoning Province, China.
authors: Yuanyi
authors: Y
authors: Wei
authors: Department of Pathology, Dalian Medical University, Dalian, China; The Key Laboratory of Tumor Metastasis in Liaoning Province, Dalian, Liaoning Province, China.
authors: Yuhong
authors: Y
authors: Huang
authors: The Key Laboratory of Tumor Metastasis in Liaoning Province, Dalian, Liaoning Province, China. Electronic address: jwtang_53@sina.cn.
authors: Jianwu
authors: J
authors: Tang
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D018118:Chloride Channels / Q000378:metabolism*; D015536:Down-Regulation; D018260:Gelsolin / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D023041:Xenograft Model Antitumor Assays
chemical_list: D017310:Annexin A7; D018118:Chloride Channels; C501967:Clic1 protein, mouse; D018260:Gelsolin
keywords: Annexin A7; Chloride intracellular channel 1(Clic1); Gelsolin; Hepatocarcinoma