title: Anti-cancer vaccination by transdermal delivery of antigen peptide-loaded nanogels via iontophoresis.
abstract: Transdermal vaccination with cancer antigens is expected to become a useful anti-cancer therapy. However, it is difficult to accumulate enough antigen in the epidermis for effective exposure to Langerhans cells because of diffusion into the skin and muscle. Carriers, such as liposomes and nanoparticles, may be useful for the prevention of antigen diffusion. Iontophoresis, via application of a small electric current, is a noninvasive and efficient technology for transdermal drug delivery. Previously, we succeeded in the iontophoretic transdermal delivery of liposomes encapsulating insulin, and accumulation of polymer-based nanoparticle nanogels in the stratum corneum of the skin. Therefore, in the present study, we examined the use of iontophoresis with cancer antigen gp-100 peptide KVPRNQDWL-loaded nanogels for anti-cancer vaccination. Iontophoresis resulted in the accumulation of gp-100 peptide and nanogels in the epidermis, and subsequent increase in the number of Langerhans cells in the epidermis. Moreover, tumor growth was significantly suppressed by iontophoresis of the antigen peptide-loaded nanogels. Thus, iontophoresis of the antigen peptide-loaded nanogels may serve as an effective transdermal delivery system for anti-cancer vaccination.
authors: Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors: Mao
authors: M
authors: Toyoda
authors: Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors: Susumu
authors: S
authors: Hama
authors: Graduate School of Pure and Applied Sciences, University of Tsukuba, Ibaraki 305-8573, Japan.
authors: Yutaka
authors: Y
authors: Ikeda
authors: Graduate School of Pure and Applied Sciences, University of Tsukuba, Ibaraki 305-8573, Japan.
authors: Yukio
authors: Y
authors: Nagasaki
authors: Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan. Electronic address: kogure@mb.kyoto-phu.ac.jp.
authors: Kentaro
authors: K
authors: Kogure
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems; D006801:Humans; D007478:Iontophoresis; D051379:Mice; D000080385:Nanogels; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D000080385:Nanogels; D010455:Peptides; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
keywords: Anti-cancer vaccine; Iontophoresis; Nanogel; Transdermal delivery