title: A new cis-encoded sRNA, BsrH, regulating the expression of hemH gene in Brucella abortus 2308.
abstract: A total of 129 sRNA candidates were identified in Brucella abortus 2308 in our previous work, and one candidate with potential to regulate expression of hemH gene was further analyzed in this study. We found that the novel sRNA can inhibit the expression of hemH and called it BsrH (Brucella sRNA regulating HemH). The expression level of BsrH was tested in four different stress conditions. A significant upregulation was detected during the growth in acidic and Brucella minimal media, as well as in the presence of hydroxyl peroxide, while iron deficiency caused the opposite effect. As expected, BsrH strongly affected the survival ratio of the Brucella cells under iron-limitation conditions, though overexpression of BsrH did not affect Brucella virulence. Thus, we conclude that BsrH plays a regulatory role in bacterial heme biosynthesis and can be considered as the first Brucella sRNA involved in stress responses.
authors: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Xiaowei
authors: X
authors: Peng
authors: Department of Zoonosis Control, China Animal Disease Control Center, Beijing 100193, China.
authors: Hao
authors: H
authors: Dong
authors: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China wuqm@cau.edu.cn.
authors: Qingmin
authors: Q
authors: Wu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002003:Brucella abortus / Q000235:genetics* / Q000472:pathogenicity*; D002006:Brucellosis / Q000382:microbiology; D019295:Computational Biology; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000378:metabolism; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D012329:RNA, Bacterial / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D058727:RNA, Small Untranslated; D007501:Iron
keywords: Brucella abortus; cis-encoded sRNA; hemH; stress response