title: Bioelectrochemistry of heme peptide at seamless three-dimensional carbon nanotubes/graphene hybrid films for highly sensitive electrochemical biosensing.
abstract: A seamless three-dimensional hybrid film consisting of carbon nanotubes grown at the graphene surface (CNTs/G) is a promising material for the application to highly sensitive enzyme-based electrochemical biosensors. The CNTs/G film was used as a conductive nanoscaffold for enzymes. The heme peptide (HP) was immobilized on the surface of the CNTs/G film for amperometric sensing of H2O2. Compared with flat graphene electrodes modified with HP, the catalytic current for H2O2 reduction at the HP-modified CNTs/G electrode increased due to the increase in the surface coverage of HP. In addition, microvoids in the CNTs/G film contributed to diffusion of H2O2 to modified HP, resulting in the enhancement of the catalytic cathodic currents. The kinetics of the direct electron transfer from the CNTs/G electrode to compound I and II of modified HP was also analyzed.
authors: Institute of Industrial Science, University of Tokyo , Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors: Kikuo
authors: K
authors: Komori
authors: Trupti
authors: T
authors: Terse-Thakoor
authors: Ashok
authors: A
authors: Mulchandani
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D037742:Nanotubes, Carbon; D010455:Peptides; D006418:Heme; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: H2O2 sensing; amperometric biosensor; carbon nanotubes-grown graphene; heme peptide; three-dimensional film