title: [Effects of activation and blockde of GABA(A)-receptors on activity of the nitrergic system of the N. accumbens].
abstract: In Sprague-Dawley rats by means of in vivo microdialysis it was shown that intra-accumbal infusion of a GABA(A) receptor antagonist bicuculline (20, 60, 120 μM) dose-dependently increased the local level of extracellular citrulline (a NO co-product). This increase was almost completely prevented by intra-accumbal infusion of 7-nitroindazol (0.5 mM), a neuronal NO synthase inhibitor. Intra-accumbal infusion ofa GABA(A) receptor agonist muscimol at concentrations of 10, 50 μM did not affect the citrulline extracellular level in this brain area, whereas at a concentration of 150 μM, muscimol decreased the accumbal citrulline extracellular level. Intra-accumbal infusion of NMDA (100 μM), an NMDA receptor agonist, increased the local level of extracellular citrulline, and intra-accumbal muscimol infusions (10, 50, 150 μM) dose-dependently decreased the NMDA-dependent citrulline rise. The data obtained indicate for the first time that in the n. accumbens, GABA(A)-receptors might regulate basal and NMDA-evoked activity of accumbal nitrergic system.
authors: N B
authors: NB
authors: Saul'skaia
authors: E V
authors: EV
authors: Vinogradova
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002956:Citrulline / Q000819:agonists / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D058785:GABA-A Receptor Agonists / Q000494:pharmacology*; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology*; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D017551:Microdialysis; D009118:Muscimol / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D007191:Indazoles; D011963:Receptors, GABA-A; D009118:Muscimol; D002956:Citrulline; D009569:Nitric Oxide; D016202:N-Methylaspartate; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; C080122:7-nitroindazole; D001640:Bicuculline