title: Principal components analysis: an innovative approach to establish interferences in ochratoxin A detection.
abstract: This work aimed to establish an innovative approach to evaluate the effect of cereals composition on ochratoxin A extraction by multivariate analysis. Principal components analysis was applied to identify the effect of major matrix components on the recovery of ochratoxin A by QuEChERS method using HPTLC and HPLC, and to validate the method for ochratoxin A determination in wheat flour by HPLC. The matrices rice bran, wheat bran and wheat flour were characterized for their physical and chemical attributes. The ochratoxin A recovery in these matrices was highly influenced (R=0.99) by the sugar content of the matrix, while the lipids content showed a minor interference (R=0.29). From these data, the QuEChERS method was standardized for extracting ochratoxin A from flour using 1% ACN:water (2:1) as extraction solvent and dried magnesium sulfate and sodium chloride as salts. The recovery values ranged from 97.6% to 105%. The validated method was applied to evaluate natural occurrence of ochratoxin A in 20 wheat flour samples, which were contaminated with ochratoxin A levels in the range of 0.22-0.85 μg kg(-1).
authors: Laboratório de Ciência de Alimentos, Escola de Química e Alimentos, Universidade Federal do Rio Grande - FURG, Rua Eng. Alfredo Huch, 475, Centro, 96201-900 Rio Grande, RS, Brazil. Electronic address: larinekupski@yahoo.com.br.
authors: L
authors: L
authors: Kupski
authors: Laboratório de Ciência de Alimentos, Escola de Química e Alimentos, Universidade Federal do Rio Grande - FURG, Rua Eng. Alfredo Huch, 475, Centro, 96201-900 Rio Grande, RS, Brazil.
authors: E
authors: E
authors: Badiale-Furlong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004043:Dietary Fiber / Q000032:analysis; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D025341:Principal Component Analysis; D014908:Triticum / Q000737:chemistry*
chemical_list: D004043:Dietary Fiber; D009793:Ochratoxins; C025589:ochratoxin A
keywords: Ochratoxin; PCA; QuEChERS; Validation; Wheat flour