title: Development of rice bran treatment process and its use for the synthesis of polyhydroxyalkanoates from rice bran hydrolysate solution.
abstract: Rice bran treatment process for the production of 43.7 kg of hydrolysate solution containing 24.41 g/L of glucose and small amount of fructose from 5 kg of rice bran was developed and employed to produce polyhydroxyalkanoates in recombinant Escherichia coli and Ralstonia eutropha strains. Recombinant E. coli XL1-Blue expressing R. eutropha phaCAB genes and R. eutropha NCIMB11599 could produce poly(3-hydroxybutyrate) with the polymer contents of 90.1 wt% and 97.2 wt%, respectively, when they were cultured in chemically defined MR medium and chemically defined nitrogen free MR medium containing 10 mL/L of rice bran hydrolysate solution, respectively. Also, recombinant E. coli XL1-Blue and recombinant R. eutropha 437-540, both of which express the Pseudomonas sp. phaC1437 gene and the Clostridium propionicum pct540 gene could produce poly(3-hydroxybutyrate-co-lactate) from rice bran hydrolysate solution. These results suggest that rice bran may be a good renewable resource for the production of biomass-based polymers by recombinant microorganisms.
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Young Hoon
authors: YH
authors: Oh
authors: Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors: Seung Hwan
authors: SH
authors: Lee
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Young-Ah
authors: YA
authors: Jang
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea; Department of Chemical System Engineering, Hongik University, Jochiwon, Chungnam 339-701, Republic of Korea.
authors: Jae Woo
authors: JW
authors: Choi
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Kyung Sik
authors: KS
authors: Hong
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Ju Hyun
authors: JH
authors: Yu
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Jihoon
authors: J
authors: Shin
authors: Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Bong Keun
authors: BK
authors: Song
authors: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido 449-728, Republic of Korea.
authors: Shaik G
authors: SG
authors: Mastan
authors: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido 449-728, Republic of Korea.
authors: Yokimiko
authors: Y
authors: David
authors: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido 449-728, Republic of Korea.
authors: Mary Grace
authors: MG
authors: Baylon
authors: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Department of Bio and Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: leesy@kaist.ac.kr.
authors: Sang Yup
authors: SY
authors: Lee
authors: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido 449-728, Republic of Korea. Electronic address: parksj93@mju.ac.kr.
authors: Si Jae
authors: SJ
authors: Park
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D020118:Cupriavidus necator / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D053858:Metabolic Networks and Pathways; D012275:Oryza / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011995:Recombination, Genetic / Q000235:genetics; D012996:Solutions; D013997:Time Factors; D014866:Waste Products
chemical_list: D054813:Polyhydroxyalkanoates; D012996:Solutions; D014866:Waste Products
keywords: Escherichia coli; Poly(3-hydroxybutyrate); Poly(3-hydroxybutyrate-co-lactate); Ralstonia eutropha; Rice bran pretreatment