title: Tnf-α inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy.
abstract: Contrast medium-induced nephropathy (CIN) remains as a problem with high incidence and mortality rates. The aim of this study is to examine the roles of infliximab (INF) in the glycerol (GLY) and CIN model in rats. The rats were separated into five groups (n=8): Healthy, GLY, GLY+CM, GLY+CM+INF 5mg/kg intraperitoneally (i.p.), and GLY+CM+INF 7 mg/kg (i.p.). Antioxidant levels in the therapy groups were observed to be quite similar to those in the healthy group. In this study, while the kidney TNF-α, IL-1β, TGF-1β and Caspase 3 gene expressions' levels increased in the nephrotoxic groups, these levels were found to have decreased in the treatment groups. Moreover, histopathologic examination showed that hyaline, haemorrhagic casts and necrosis were increased in nephrotoxicity group, whereas they decreased in the therapy group. Furthermore, TNF-α and NF-κB expression were decreased with infliximab administrated groups similar to control group. In conclusion, we suggest that infliximab have protective roles on CIN.
authors: Department of Emergency Medicine; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
authors: Murat
authors: M
authors: Saritemur
authors: Department of Biochemistry; Agri Ibrahim Cecen University Faculty of Pharmacy, 04100 Agri Turkey.
authors: Harun
authors: H
authors: Un
authors: Department of Pharmacology; Ataturk University Faculty of Pharmacy, 25240 Erzurum Turkey. Electronic address: ecadirci@atauni.edu.tr.
authors: Elif
authors: E
authors: Cadirci
authors: Department of Pharmacology; Ataturk University Faculty of Veterinary Medicine, 25240 Erzurum Turkey.
authors: Emre
authors: E
authors: Karakus
authors: Department of Pharmacology; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
authors: Erol
authors: E
authors: Akpinar
authors: Department of Pharmacology; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
authors: Zekai
authors: Z
authors: Halici
authors: Department of Pharmacology; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
authors: Rustem Anil
authors: RA
authors: Ugan
authors: Department of Radiology; Ataturk University Faculty of Medicine, 25240 Erzurum Turkey.
authors: Adem
authors: A
authors: Karaman
authors: Department of Pathology; Kırıkkale University Faculty of Veterinary, 71450 Kirikkale Turkey.
authors: Hasan Tarik
authors: HT
authors: Atmaca
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001806:Blood Urea Nitrogen; D053148:Caspase 3 / Q000235:genetics; D003287:Contrast Media; D003404:Creatinine / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D005990:Glycerol; D000069285:Infliximab / Q000494:pharmacology / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000235:genetics; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; D003287:Contrast Media; D053583:Interleukin-1beta; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D003404:Creatinine; D000069285:Infliximab; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione; D005990:Glycerol
keywords: Contrast-media; Infliximab; Nephrotoxicity; Rat