title: Nanocomplexation of thrombin with cationic amylose derivative for improved stability and hemostatic efficacy.
abstract: As a topical hemostatic agent, thrombin has wide application for many surgical treatments. However, native thrombin always suffers from its physical and chemical instabilities. In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system. For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated. Their stabilities were studied in terms of activity retention percentages under different storage time, pH values, and illumination time. In addition, their ability to achieve in vitro fibrinogen and blood coagulation were evaluated. Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Baoxiong
authors: B
authors: Zhuang
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Zhihua
authors: Z
authors: Li
authors: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Jiadong
authors: J
authors: Pang
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Wenbin
authors: W
authors: Li
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Pinbo
authors: P
authors: Huang
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Jie
authors: J
authors: Wang
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Yu
authors: Y
authors: Zhou
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Qing
authors: Q
authors: Lin
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Quanbo
authors: Q
authors: Zhou
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Xiao
authors: X
authors: Ye
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Huilin
authors: H
authors: Ye
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Yimin
authors: Y
authors: Liu
authors: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Li-Ming
authors: LM
authors: Zhang
authors: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors: Rufu
authors: R
authors: Chen
mesh_terms: D000688:Amylose / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002412:Cations / Q000737:chemistry / Q000494:pharmacology; D004355:Drug Stability; D005340:Fibrinogen / Q000737:chemistry; D006470:Hemorrhage / Q000188:drug therapy; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology; D007083:Iliac Artery / Q000293:injuries; D049329:Nanostructures / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D013917:Thrombin / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002412:Cations; D006490:Hemostatics; D011107:Polylysine; D005340:Fibrinogen; D000688:Amylose; D013917:Thrombin
keywords: amylose derivative; complexation; hemostatic activity; nanoparticles; stability; thrombin