title: CD8+ T lymphocyte expansion, proliferation and activation in dengue fever.
abstract: Dengue fever induces a robust immune response, including massive T cell activation. The level of T cell activation may, however, be associated with more severe disease. In this study, we explored the level of CD8+ T lymphocyte activation in the first six days after onset of symptoms during a DENV2 outbreak in early 2010 on the coast of São Paulo State, Brazil. Using flow cytometry we detected a progressive increase in the percentage of CD8+ T cells in 74 dengue fever cases. Peripheral blood mononuclear cells from 30 cases were thawed and evaluated using expanded phenotyping. The expansion of the CD8+ T cells was coupled with increased Ki67 expression. Cell activation was observed later in the course of disease, as determined by the expression of the activation markers CD38 and HLA-DR. This increased CD8+ T lymphocyte activation was observed in all memory subsets, but was more pronounced in the effector memory subset, as defined by higher CD38 expression. Our results show that most CD8+ T cell subsets are expanded during DENV2 infection and that the effector memory subset is the predominantly affected sub population.
authors: Universidade de São Paulo, Faculdade de Medicina, Disciplina de Imunologia Clínica e Alergia (LIM-60), São Paulo, Brazil.
authors: Andréia Manso
authors: AM
authors: de Matos
authors: Universidade de São Paulo, Faculdade de Medicina, Disciplina de Imunologia Clínica e Alergia (LIM-60), São Paulo, Brazil; Albert Einstein Hospital, São Paulo, Brazil.
authors: Karina Inacio
authors: KI
authors: Carvalho
authors: Universidade de São Paulo, Faculdade de Medicina, Disciplina de Imunologia Clínica e Alergia (LIM-60), São Paulo, Brazil; Division of Immunology, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil.
authors: Daniela Santoro
authors: DS
authors: Rosa
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: Lucy Santos
authors: LS
authors: Villas-Boas
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: Wanessa Cardoso
authors: WC
authors: da Silva
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: Célia Luiza de Lima
authors: CL
authors: Rodrigues
authors: Hospital Ana Costa, Santos, São Paulo, Brazil.
authors: Olímpia Massae Nakasone Peel Furtado
authors: OM
authors: Oliveira
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: José Eduardo
authors: JE
authors: Levi
authors: Hospital Ana Costa, Santos, São Paulo, Brazil; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias (LIM-47), São Paulo, Brazil.
authors: Evaldo Stanislau Affonso
authors: ES
authors: Araújo
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: Claudio Sergio
authors: CS
authors: Pannuti
authors: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo e Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias-(LIM-52), São Paulo, Brazil.
authors: Expedito José Albuquerque
authors: EJ
authors: Luna
authors: Universidade de São Paulo, Faculdade de Medicina, Disciplina de Imunologia Clínica e Alergia (LIM-60), São Paulo, Brazil.
authors: Esper George
authors: EG
authors: Kallas
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D001938:Brazil; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D019394:Ki-67 Antigen / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D006684:HLA-DR Antigens; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1