title: Murine gammaherpesvirus (MHV-68) transforms cultured cells in vitro.
abstract: Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype ('criss-cross' pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5-6 passages (35-40 days), and then they entered into crisis and most of them died. In a small number of cultures, however, foci of newly transformed cells appeared from which two stable cell lines were derived. After 6-9 cell culture passages of the MHV-68 transformed cell lines, MHV-68 DNA and virus antigen could be detected by PCR and immunofluorescence assay along with the disappearance of actin bundles, indicating that both transformed cell lines might be oncogenic.
authors: Department of Microbiology and Virology, Faculty of Natural Science, Comenius University, Bratislava, Slovakia.
authors: Veronika
authors: V
authors: Mrázová
authors: Tatiana
authors: T
authors: Betáková
authors: Marcela
authors: M
authors: Kúdelová
authors: Miroslava
authors: M
authors: Šupolíková
authors: Veronika
authors: V
authors: Lachová
authors: Barbora
authors: B
authors: Lapuníková
authors: František
authors: F
authors: Golais
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D041681:NIH 3T3 Cells; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018144:Rhadinovirus / Q000502:physiology*; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral