title: Mannan-binding lectin reduces CpG DNA-induced inflammatory cytokine production by human monocytes.
abstract: Mannan-binding lectin (MBL) belongs to the collectin family and functions as an opsonin that can also initiate complement activation. Our previous study showed that MBL serves as a double-stranded RNA binding protein that attenuates polyriboinosinic-polyribocytidylic acid-induced TLR3 activation. Prompted by these findings, in the present study cross-talk between MBL and CpG-DNA-induced TLR9 activation was investigated. Here, it was found that MBL also interacts with the TLR9 agonist, CpG oligodeoxynucleotide (CpG-ODN), in a calcium-dependent manner. Purified MBL protein suppressed activation of nuclear factor-kappa B signaling and subsequent production of proinflammatory cytokines from human monocytes induced by CpG-ODN 2006. These observations indicate that MBL can down-regulate CpG DNA-induced TLR9 activation, emphasizing the importance of understanding the interaction of MBL with TLR agonist in host immune defense.
authors: Department of Immunology, Southern Medical University, Guangzhou, 510515.
authors: Yuan
authors: Y
authors: Tang
authors: Di
authors: D
authors: Ma
authors: Siqi
authors: S
authors: Ming
authors: Liyun
authors: L
authors: Zhang
authors: Jia
authors: J
authors: Zhou
authors: Guiqiu
authors: G
authors: Shan
authors: Zhengliang
authors: Z
authors: Chen
authors: Xiao
authors: X
authors: Lu
authors: Daming
authors: D
authors: Zuo
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D037601:Mannose-Binding Lectin / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D051217:Toll-Like Receptor 9 / Q000276:immunology
chemical_list: C408982:CPG-oligonucleotide; D016207:Cytokines; D037601:Mannose-Binding Lectin; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D051217:Toll-Like Receptor 9
keywords: CpG oligodeoxynucleotides; Toll like receptor 9; mannan binding lectin