title: Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
abstract: Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios. Thereafter, the composites were subjected to γ-radiation sterilization. The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF). We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites. γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties. The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction. Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
authors: Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, University of Dhaka, Dhaka 1000, Bangladesh.
authors: Md Minhajul
authors: MM
authors: Islam
authors: Institute of Radiation and Polymer Technology (IRPT), Atomic Energy Research Establishment (AERE), P. O. Box No. 3787, Dhaka 1000, Bangladesh.
authors: Mubarak A
authors: MA
authors: Khan
authors: Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, University of Dhaka, Dhaka 1000, Bangladesh. Electronic address: mizanur.rahman@du.ac.bd.
authors: Mohammed Mizanur
authors: MM
authors: Rahman
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D005720:Gamma Rays; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013718:Tensile Strength / Q000187:drug effects; D013818:Thermogravimetry / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011108:Polymers; C485817:beta-tricalcium phosphate; D005780:Gelatin; D048271:Chitosan
keywords: Biocomposite; Bone tissue engineering; Chitosan; Gelatin; γ-Radiation sterilization