title: RPN1a, a subunit of the 26S proteasome, controls trichome development in Arabidopsis.
abstract: The ubiquitin-mediated 26S proteasome pathway (UPS) is of great importance to plant growth and development. Previously research showed that a subunit of the 26S proteasome, named RPN1a, was involved in trichome's branching in Arabidopsis. Mutation in RPN1a give rise to more trichome branches on leaves. Here, we found that T-DNA insertion mutation in RPN1a resulted in increased trichome branches on main stem, and trichome number on rosette leaves and the main stem compared with the wild type plant. Expression analysis results showed that the transcription levels of ZFP6, ZFP5, GIS, GL1, GL2, GL3, TTG1 and MYB23, which promote trichome initiation, were up-regulated in the rpn1a mutant, and expression of FRC4, which is responsible for increased trichome branching, was also increased in the rpn1a mutant. Moreover, the mRNA expression level of RPN1a was significantly repressed by GA (gibberellin) and CK (cytokinin) treatment, which are two important phytohormones that play essential roles in trichome development. These results demonstrate that RPN1a may be involved in trichome development through the GA and CK signaling pathways.
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors: Dashi
authors: D
authors: Yu
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors: Feng
authors: F
authors: Yu
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors: Changqing
authors: C
authors: Du
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors: Xiushan
authors: X
authors: Li
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China. Electronic address: zxy_mm@163.com.
authors: Xiaoying
authors: X
authors: Zhao
authors: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China. Electronic address: xmL05@hnu.edu.cn.
authors: Xuanming
authors: X
authors: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism; D004269:DNA, Bacterial; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves; D018547:Plant Stems; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015533:Transcriptional Activation; D064201:Trichomes / Q000254:growth & development*; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D004269:DNA, Bacterial; D005875:Gibberellins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C507997:RPN1 protein, Arabidopsis; C036483:T-DNA; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease
keywords: 26S proteasome subunit; Arabidopsis; RPN1a; Trichome