title: Photo-induced conjugation of tetrazoles to modified and native proteins.
abstract: Bio-orthogonal chemistry has been widely used for conjugation of polymer molecules to proteins. Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. During the course of the investigation, a significant side-reaction was found to occur for the conjugation of PEG-tetrazole to native BLG. Further exploration of the underlying chemistry reveals that the presence of a tryptophan residue is sufficient for conjugation of tetrazole-modified molecules.
authors: Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore. MNallani@ntu.edu.sg.
authors: Winna
authors: W
authors: Siti
authors: Amit Kumar
authors: AK
authors: Khan
authors: Hans-Peter M
authors: HP
authors: de Hoog
authors: Bo
authors: B
authors: Liedberg
authors: Madhavan
authors: M
authors: Nallani
mesh_terms: D000818:Animals; D002417:Cattle; D007782:Lactoglobulins / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D055668:Photochemical Processes; D011092:Polyethylene Glycols / Q000737:chemistry; D013777:Tetrazoles / Q000737:chemistry*
chemical_list: D007782:Lactoglobulins; D013777:Tetrazoles; D011092:Polyethylene Glycols