title: Direct patterning of nanoparticles and biomolecules by liquid nanodispensing.
abstract: We report on the localized deposition of nanoparticles and proteins, nano-objects commonly used in many nanodevices, by the liquid nanodispensing (NADIS) technique which consists in depositing droplets of a solution through a nanochannel drilled at the apex of an AFM tip. We demonstrate that the size of spots can be adjusted from microns down to sub-50 nm by tuning the channel diameter, independently of the chemical nature of the solute. In the case of nanoparticles, we demonstrated the ultimate limit of the method and showed that large arrays of single (or pairs of) nanoparticles can be reproducibly deposited. We further explored the possibility to deposit different visible fluorescent proteins using NADIS without loss of protein function. The intrinsic fluorescence of these proteins is characteristic of their structural integrity; the retention of fluorescence after NADIS deposition demonstrates that the proteins are intact and functional. This study demonstrates that NADIS can be a viable alternative to other scanning probe lithography techniques since it combines high resolution direct writing of nanoparticles or biomolecules with the versatility of liquid lithography techniques.
authors: Nanosciences Group, CEMES-CNRS, 29 rue Jeanne Marvig, 31055 Toulouse cedex 5, France. thierry.ondarcuhu@cemes.fr.
authors: Laure
authors: L
authors: Fabié
authors: Pierre
authors: P
authors: Agostini
authors: Martijn
authors: M
authors: Stopel
authors: Christian
authors: C
authors: Blum
authors: Benjamin
authors: B
authors: Lassagne
authors: Vinod
authors: V
authors: Subramaniam
authors: Thierry
authors: T
authors: Ondarçuhu
mesh_terms: D049452:Green Fluorescent Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D049452:Green Fluorescent Proteins