title: Hypoglycemic activity evaluation and chemical study on hollyhock flowers.
abstract: Hollyhock (Althaea rosea (Linn.) Cavan) belongs to Althaea genus, Malvaceae family, is a perennial garden plant distributed throughout the world in warm temperate and tropical regions, which was used as anti-diabetes ingredient in traditional Chinese medicine. In the process of our research, ethanolic extract of hollyhock flower (HFE) was found to decrease serum triglyceride and glucose levels significantly in KK-A(y) mice after oral administration for 8weeks. Meanwhile, gene expressions on AMPK, IRS2, PI3K, AKT and GLUT4 in liver were remarkably up-regulated. Three new dihydroflavonol glycosides, named as roseaflavanonolosides A (1), B (2), and C (3), together with two known ones were obtained from HFE. Their structures were elucidated by chemical and spectroscopic methods. Hepatic cell glucose uptake experiment was performed using 2-NBDG as a glucose uptake indicator. At the dosage of 20μg/mL for 1-5, the glucose uptake increasing level was nearly 30%-40% in HepG2 cells. We partly revealed the glucose metabolism regulation effect may relate to dihydroflavonols in hollyhock flower.
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Yi
authors: Y
authors: Zhang
authors: Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Lijun
authors: L
authors: Jin
authors: Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine), Ministry of Education, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Qiu
authors: Q
authors: Chen
authors: Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Zhizhen
authors: Z
authors: Wu
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Yongzhe
authors: Y
authors: Dong
authors: Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine), Ministry of Education, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors: Lifeng
authors: L
authors: Han
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, PR China. Electronic address: wangtao@tjutcm.edu.cn.
authors: Tao
authors: T
authors: Wang
mesh_terms: D031583:Althaea / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D044948:Flavonols; D006027:Glycosides; D007004:Hypoglycemic Agents; D014280:Triglycerides; C000598361:roseaflavanonoloside A
keywords: Althaea rosea; Dihydroflavonol glycosides; Glucose and lipid regulation; Glucose uptake; HepG2 cells; Hollyhock flower