title: Enhanced expression of heterologous proteins in yeast cells via the modification of N-glycosylation sites.
abstract: Yeasts are widely used for the production of heterologous proteins. Improving the expression of such proteins is a top priority for pharmaceutical and industrial applications. N-Glycosylation, a common form of protein modification in yeasts, facilitates proper protein folding and secretion. Accordingly, our previous study revealed that the attachment of additional N-glycans to recombinant elastase by introducing an N-glycosylation sequon at suitable locations could stimulate its expression. Interestingly, the sequon Asn-Xaa-Thr is N-glycosylated more efficiently than Asn-Xaa-Ser, so improving the N-glycosylation efficiency via the conversion of Ser to Thr in the sequon would enhance the efficiency of N-glycosylation and increase glycoprotein expression. Recently, the expression level of recombinant elastase was enhanced by this means in our lab. Actually, the modification of N-glycosylation sites can generally be achieved through site-directed mutagenesis; thus, the method described in this report represents a feasible means of improving heterologous protein expression in yeasts.
authors: a Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake; School of Life Sciences; Huaiyin Normal University ; Huaian , China.
authors: Minghai
authors: M
authors: Han
authors: Xiaobin
authors: X
authors: Yu
mesh_terms: D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D015003:Yeasts / Q000378:metabolism*
chemical_list: D006023:Glycoproteins
keywords: N-glycosylation site; heterologous protein; protein folding; protein secretion; yeast