title: Novel tools for genetic manipulation of follicle stem cells in the Drosophila ovary reveal an integrin-dependent transition from quiescence to proliferation.
abstract: In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Tiffiney R
authors: TR
authors: Hartman
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Erin M
authors: EM
authors: Ventresca
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Anthony
authors: A
authors: Hopkins
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Daniel
authors: D
authors: Zinshteyn
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Molecular Cell Biology and Genetics Graduate Program, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102.
authors: Tanu
authors: T
authors: Singh
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Department of Cancer Biology and Genetics, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
authors: Jenny A
authors: JA
authors: O'Brien
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 North Penn High School, Lansdale, Pennsylvania 19446.
authors: Benjamin C
authors: BC
authors: Neubert
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Matthew G
authors: MG
authors: Hartman
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Heather K
authors: HK
authors: Schofield
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors: Kevin P
authors: KP
authors: Stavrides
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 St. Hubert Catholic High School for Girls, Philadelphia, Pennsylvania 19136.
authors: Danielle E
authors: DE
authors: Talbot
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Science Scholars Program, Temple University, Philadelphia, Pennsylvania 19122.
authors: Devon J
authors: DJ
authors: Riggs
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Souderton Area High School, Souderton, Pennsylvania 18964.
authors: Caroline
authors: C
authors: Pritchard
authors: Program in Cancer Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Alana.OReilly@fccc.edu.
authors: Alana M
authors: AM
authors: O'Reilly
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D049750:Genome, Insect; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D006080:Ovarian Follicle / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
chemical_list: D016023:Integrins
keywords: adhesion; follicle stem cell; germarium; integrin; quiescence