title: Knockdown of a JmjC domain-containing gene JMJ524 confers altered gibberellin responses by transcriptional regulation of GRAS protein lacking the DELLA domain genes in tomato.
abstract: Plants integrate responses to independent hormonal and environmental signals to survive adversity. In particular, the phytohormone gibberellin (GA) regulates a variety of developmental processes and stress responses. In this study, the Jumonji-C (JmjC) domain-containing gene JMJ524 was characterized in tomato. JMJ524 responded to circadian rhythms and was upregulated by GA treatment. Knockdown of JMJ524 by RNAi caused a GA-insensitive dwarf phenotype with shrunken leaves and shortened internodes. However, in these transgenic plants, higher levels of endogenous GAs were detected. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two DELLA-like genes, SlGLD1 ('GRAS protein Lacking the DELLA domain') and SlGLD2, were increased in JMJ524-RNAi transgenic plants. Nevertheless, only the overexpression of SlGLD1 in tomato resulted in a GA-insensitive dwarf phenotype, suggesting that SlGLD1 acts as a repressor of GA signalling. This study proposes that JMJ524 is required for stem elongation by altering GA responses, at least partially by regulating SlGLD1.
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education; College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, P. R. China.
authors: Jinhua
authors: J
authors: Li
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China.
authors: Chuying
authors: C
authors: Yu
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China.
authors: Hua
authors: H
authors: Wu
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China.
authors: Zhidan
authors: Z
authors: Luo
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China.
authors: Bo
authors: B
authors: Ouyang
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China.
authors: Long
authors: L
authors: Cui
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China zhangjunhng@mail.hzau.edu.cn zbye@mail.hzau.edu.cn.
authors: Junhong
authors: J
authors: Zhang
authors: Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan 430070, China zhangjunhng@mail.hzau.edu.cn zbye@mail.hzau.edu.cn.
authors: Zhibiao
authors: Z
authors: Ye
mesh_terms: D000595:Amino Acid Sequence; D055785:Gene Knockdown Techniques; D005875:Gibberellins / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins
keywords: DELLA; GRAS; JmjC; dwarfism; gibberellin; tomato.