title: Mapping native disulfide bonds at a proteome scale.
abstract: We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.html), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues.
authors: 1] College of Life Science, Beijing Normal University, Beijing, China. [2] National Institute of Biological Sciences, Beijing, China.
authors: Shan
authors: S
authors: Lu
authors: 1] Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China. [2] University of Chinese Academy of Sciences, Beijing, China.
authors: Sheng-Bo
authors: SB
authors: Fan
authors: National Institute of Biological Sciences, Beijing, China.
authors: Bing
authors: B
authors: Yang
authors: National Institute of Biological Sciences, Beijing, China.
authors: Yu-Xin
authors: YX
authors: Li
authors: 1] Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China. [2] University of Chinese Academy of Sciences, Beijing, China.
authors: Jia-Ming
authors: JM
authors: Meng
authors: 1] Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China. [2] University of Chinese Academy of Sciences, Beijing, China.
authors: Long
authors: L
authors: Wu
authors: Institute of Molecular Medicine, Peking University, Beijing, China.
authors: Pin
authors: P
authors: Li
authors: 1] Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China. [2] University of Chinese Academy of Sciences, Beijing, China.
authors: Kun
authors: K
authors: Zhang
authors: National Institute of Biological Sciences, Beijing, China.
authors: Mei-Jun
authors: MJ
authors: Zhang
authors: National Center for Mathematics and Interdisciplinary Sciences, Key Laboratory of Random Complex Structures and Data Science, Academy of Mathematics and Systems Science, CAS, Beijing, China.
authors: Yan
authors: Y
authors: Fu
authors: Institute of Molecular Medicine, Peking University, Beijing, China.
authors: Jincai
authors: J
authors: Luo
authors: Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China.
authors: Rui-Xiang
authors: RX
authors: Sun
authors: Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences (CAS), Beijing, China.
authors: Si-Min
authors: SM
authors: He
authors: 1] College of Life Science, Beijing Normal University, Beijing, China. [2] National Institute of Biological Sciences, Beijing, China.
authors: Meng-Qiu
authors: MQ
authors: Dong
mesh_terms: D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D012260:Ribonucleases / Q000737:chemistry
chemical_list: D004220:Disulfides; D019151:Peptide Library; D020543:Proteome; D012260:Ribonucleases