title: Effect of Copper Sulfate on Expression of Endogenous L1 Retrotransposons in HepG2 Cells (Hepatocellular Carcinoma).
abstract: The long interspersed element-1 (LINE-1 or L1) constitutes approximately 17% of human genome. The expression of these elements is deregulated upon exposure to environmental exposures resulting to genomic instability and cancer promotion. The effect of copper as essential elements in regulation of L1 expression remained to be elucidated. Using non-cytotoxic concentrations of the copper, the expression of endogenous L1 was analyzed by qPCR after 6 days of copper pretreatment in human hepatocellular carcinoma cells (HepG2). The results indicated that the expression of active L1 elements are significantly downregulated at concentrations of 12.5, 25, and 50 μM (p < 0.005). Our data imply that low-level copper exposure may have a protective effect to suppress the induction of L1 activity and decrease incidence of cancer-associated L1 mutagenesis. If this achievement is confirmed by further studies, it can be applied in the long-term goals of cancer prevention.
authors: Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Etemadzadeh Ave., West Fatemi, Tehran, Iran.
authors: Abbas
authors: A
authors: Karimi
authors: Keivan
authors: K
authors: Majidzadeh-A
authors: Zahra
authors: Z
authors: Madjd
authors: Abolfazl
authors: A
authors: Akbari
authors: Laleh
authors: L
authors: Habibi
authors: Seyed Mohammad
authors: SM
authors: Akrami
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D019327:Copper Sulfate / Q000737:chemistry*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015894:Genome, Human; D042822:Genomic Instability; D056945:Hep G2 Cells; D006706:Homeostasis; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D020084:Long Interspersed Nucleotide Elements / Q000187:drug effects*; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D011379:Prognosis
chemical_list: D019327:Copper Sulfate