title: Liquid-based cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples.
abstract: Liquid-based Cytology (LBC) consists of immediate wet cell fixation with automated slide preparation. We applied LBC, cell block (CB) and immunocytochemistry to diagnose canine lymphoma and compare results with conventional cytology. Samples from enlarged lymph nodes of 18 dogs were collected and fixed in preservative solution for automated slide preparation (LBC), CB inclusion and immunophenotyping. Two CB techniques were tested: fixed sediment method (FSM) and agar method (AM). Anti-CD79a, anti-Pax5, anti-CD3 and anti-Ki67 were used in immunocytochemistry. LBC smears showed better nuclear and nucleolar definition, without cell superposition, but presented smaller cell size and worse cytoplasmic definition. FSM showed consistent cellular groups and were employed for immunocytochemistry, while AM CBs presented sparse groups of lymphocytes, with compromised analysis. Anti-Pax-5 allowed B-cell identification, both in reactive and neoplastic lymph nodes. Our preliminary report suggests that LBC and FSM together may be promising tools to improve lymphoma diagnosis through fine-needle aspiration.
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: N C C A
authors: NC
authors: Fernandes
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: J M
authors: JM
authors: Guerra
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: R A
authors: RA
authors: Réssio
authors: HISTOPET, São Paulo, Brasil.
authors: D G
authors: DG
authors: Wasques
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: D
authors: D
authors: Etlinger-Colonelli
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: S
authors: S
authors: Lorente
authors: Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors: E
authors: E
authors: Nogueira
authors: Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil.
authors: M L Z
authors: ML
authors: Dagli
mesh_terms: D000818:Animals; D044963:Biopsy, Fine-Needle / Q000379:methods / Q000662:veterinary; D018844:Cyclin-Dependent Kinases; D003584:Cytological Techniques / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D016130:Immunophenotyping / Q000662:veterinary; D008198:Lymph Nodes / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008297:Male
chemical_list: D018844:Cyclin-Dependent Kinases
keywords: Cancer; Cytological Techniques; Dog; Lymphoma