title: Lactic acid bacteria as a surface display platform for Campylobacter jejuni antigens.
abstract: BACKGROUND
  Food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. Campylobacter spp. is a very widespread cause of gastroenteritis. Over the past decade there has been increasing interest in the use of lactic acid bacteria (LAB) as mucosal delivery vehicles. They represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens.
  
  
  METHODS
  We examined the binding ability of hybrid proteins to nontreated or trichloroacetic acid (TCA)-pretreated LAB cells by immunofluorescence and Western blot analysis.
  
  
  RESULTS
  In this study we evaluated the possibility of using GEM (Gram-positive enhancer matrix) particles of Lactobacillus salivarius as a binding platform for 2 conserved, immunodominant, extracytoplasmic Campylobacter jejuni proteins: CjaA and CjaD. We analyzed the binding ability of recombinant proteins that contain C. jejuni antigens (CjaA or CjaD) fused with the protein anchor (PA) of the L. lactis peptidoglycan hydrolase AcmA, which comprises 3 LysM motifs and determines noncovalent binding to the cell wall peptidoglycan. Both fused proteins, i.e. 6HisxCjaAx3LysM and 6HisxCjaDx3LysM, were able to bind to nontreated or TCA-pretreated L. salivarius cells.
  
  
  CONCLUSIONS
  Our results documented that the LysM-mediated binding system allows us to construct GEM particles that present 2 C. jejuni antigens.
authors: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors: Patrycja
authors: P
authors: Kobierecka
authors: Agnieszka
authors: A
authors: Wyszyńska
authors: Marta
authors: M
authors: Maruszewska
authors: Anna
authors: A
authors: Wojtania
authors: Joanna
authors: J
authors: Żylińska
authors: Jacek
authors: J
authors: Bardowski
authors: Elżbieta Katarzyna
authors: EK
authors: Jagusztyn-Krynicka
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism*; D061505:Cell Surface Display Techniques / Q000379:methods*; D004337:Drug Carriers / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D004337:Drug Carriers; D011994:Recombinant Proteins