title: Two waves of proteasome-dependent protein degradation in the hippocampus are required for recognition memory consolidation.
abstract: Healthy neuronal function and synaptic modification require a concert of synthesis and degradation of proteins. Increasing evidence indicates that protein turnover mediated by proteasome activity is involved in long-term synaptic plasticity and memory. However, its role in different phases of memory remains debated, and previous studies have not examined the possible requirement of protein degradation in recognition memory. Here, we show that the proteasome inhibitor, lactacystin (LAC), infused into the CA1 area of the hippocampus at two specific time points during consolidation, impairs 24-retention of memory for object recognition in rats. Administration of LAC after retrieval did not affect retention. These findings provide the first evidence for a requirement of proteasome activity in recognition memory, indicate that protein degradation in the hippocampus is necessary during selective time windows of memory consolidation, and further our understanding of the role of protein turnover in memory formation.
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil; National Institute for Translational Medicine, 90035-003 Porto Alegre, RS, Brazil.
authors: Luciana S
authors: LS
authors: Figueiredo
authors: National Institute for Translational Medicine, 90035-003 Porto Alegre, RS, Brazil; Laboratory of Neuropharmacology and Neural Tumor Biology, Department of Pharmacology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil; Cancer Research Laboratory, University Hospital Research Center (CPE-HCPA), Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil.
authors: Arethuza S
authors: AS
authors: Dornelles
authors: National Institute for Translational Medicine, 90035-003 Porto Alegre, RS, Brazil; Laboratory of Neuropharmacology and Neural Tumor Biology, Department of Pharmacology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil; Cancer Research Laboratory, University Hospital Research Center (CPE-HCPA), Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil.
authors: Fernanda S
authors: FS
authors: Petry
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil.
authors: Lucio
authors: L
authors: Falavigna
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil.
authors: Vinicius A
authors: VA
authors: Dargél
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil.
authors: Luiza M
authors: LM
authors: Köbe
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil.
authors: Cristiano
authors: C
authors: Aguzzoli
authors: National Institute for Translational Medicine, 90035-003 Porto Alegre, RS, Brazil; Laboratory of Neuropharmacology and Neural Tumor Biology, Department of Pharmacology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil; Cancer Research Laboratory, University Hospital Research Center (CPE-HCPA), Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil.
authors: Rafael
authors: R
authors: Roesler
authors: Neurobiology and Developmental Biology Laboratory, Faculty of Biosciences, Pontifical Catholic University, 90619-900 Porto Alegre, RS, Brazil; National Institute for Translational Medicine, 90035-003 Porto Alegre, RS, Brazil. Electronic address: nadja.schroder@pucrs.br.
authors: Nadja
authors: N
authors: Schröder
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D057967:Infusions, Intraventricular; D008297:Male; D000069077:Memory Consolidation / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D059748:Proteolysis; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000502:physiology*; D012153:Retention, Psychology / Q000502:physiology
chemical_list: C067713:lactacystin; D046988:Proteasome Endopeptidase Complex; D000111:Acetylcysteine
keywords: Hippocampus; Lactacystin; Object recognition; Proteasome; Protein degradation; Recognition memory