title: Cloning and heterologous expression of cellulose free thermostable xylanase from Bacillus brevis.
abstract: Xylanase gene isolated from Bacillus brevis was expressed in E. coli BL21. Sequencing of the gene (Gen Bank accession number: HQ179986) showed that it belongs to family 11 xylanases. The recombinant xylanase was predominantly secreted to culture medium and showed mesophilic nature (optimum activity at 55°C and pH 7.0). The cell free culture medium exhibited 30 IU/ml xylanse activity. The enzyme did not show any cellulose activity and was active under wide range of temperature (40°C to 80°C) and pH (4 to 9). The enzyme showed considerable thermo stability and regained over 90% of activity, when returned to 55°C after boiling for 5 min. These physiochemical properties of B. brevis xylanse show high potential of its applications in paper and pulp industry.
authors: Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, 302001 India.
authors: Girish K
authors: GK
authors: Goswami
authors: Department of Biotechnology, Birla Institute of Scientific Research, Jaipur, 302001 India.
authors: Medichtrla
authors: M
authors: Krishnamohan
authors: Department of Biotechnology, Gautam Buddha University, Greater NOIDA, 201308 India.
authors: Vikrant
authors: V
authors: Nain
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors: Chetana
authors: C
authors: Aggarwal
authors: Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004 India.
authors: Bandarupalli
authors: B
authors: Ramesh