title: The effect of mono-(2-ethylhexyl) phthalate on apoptosis of rat ovarian granulosa cells in vitro.
abstract: Mono-(2-ethylhexyl) phthalate (MEHP), the active metabolite of di-(2-ethylhexyl) phthalate (DEHP), is considered to be a reproductive toxicant. This study was performed to evaluate the effect of MEHP on apoptosis of rat ovarian granulosa cells and explore potential mechanism. Granulosa cells were treated with MEHP (0, 25, 50, and 100 μmol/l). Inhibited cell viability and increased apoptosis rate were observed in 50 and 100 μmol/l groups. CASPASE3 activity and BAX expression were significantly raised in all MEHP-treated groups; BCL2 expression was elevated in 25 μmol/l group, while inhibited in 50 and 100 μmol/l groups; BAX/BCL2 ratio was increased in a typical dose-effect relationship. In conclusion, this study showed that MEHP exposure induced cell viability decrease and apoptosis, associated with increase of CASPASE3 activity and BAX/BCL2 ratio. Moreover, CASPASE3 activity showed a reversed dose-dependent effect in MEHP-treated groups, indicating there might exist other CASPASE-independent pathway involved in MEHP-induced apoptosis.
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China; The Second Hospital of Dalian Medical University, Dalian, Liaoning 116012, China.
authors: Na
authors: N
authors: Li
authors: Department of Preventive Medicine, School of Public Health, Jilin University, Changchun, Jilin 130000, China; Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Keqiang
authors: K
authors: Liu
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Haitao
authors: H
authors: Yuan
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Jian
authors: J
authors: Zhu
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Guangyan
authors: G
authors: Yu
authors: Department of Radiology, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Jingli
authors: J
authors: Xie
authors: Department of Preventive Medicine, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Songpo
authors: S
authors: Fu
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China.
authors: Kun
authors: K
authors: Guo
authors: Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin 130000, China. Electronic address: jlyelin@163.com.
authors: Lin
authors: L
authors: Ye
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D004051:Diethylhexyl Phthalate; C505410:Casp3 protein, rat; D053148:Caspase 3; C016599:mono-(2-ethylhexyl)phthalate
keywords: Apoptosis; Granulosa cells; Mono-(2-ethylhexyl) phthalate; Reproductive toxicity