title: Hydrothermal and wet disk milling pretreatment for high conversion of biosugars from oil palm mesocarp fiber.
abstract: Eco-friendly pretreatment methods for lignocellulosic biomass are being developed as alternatives to chemical based methods. Superheated steam (SHS), hot compressed water (HCW) and wet disk milling (WDM) were used individually and with combination to partially remove hemicellulose and alter the lignin composition of recalcitrant structure of oil palm mesocarp fiber (OPMF). The efficiency of the pretreatment methods was evaluated based on the chemical compositions altered, SEM analysis, power consumption and degree of enzymatic digestibility. Hemicellulose removal (94.8%) was more pronounced under HCW compared to SHS, due to maximal contact of water and production of acetic acid which enhanced further degradation of hemicellulose. Subsequent treatment with WDM resulted in defibrillation of OPMF and expansion of the specific surface area thus increasing the conversion of cellulose to glucose. The highest glucose yield was 98.1% (g/g-substrate) when pretreated with HCW (200 °C, 20 min) and WDM which only consumed 9.6 MJ/kg of OPMF.
authors: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: rafein-zakaria@aist.go.jp.
authors: Mohd Rafein
authors: MR
authors: Zakaria
authors: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Mohd Nor Faiz
authors: MN
authors: Norrrahim
authors: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
authors: Satoshi
authors: S
authors: Hirata
authors: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Mohd Ali
authors: MA
authors: Hassan
mesh_terms: D028023:Arecaceae / Q000737:chemistry / Q000648:ultrastructure; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D005638:Fruit / Q000737:chemistry* / Q000648:ultrastructure; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D013227:Steam; D013696:Temperature; D013997:Time Factors; D014867:Water; D014994:Xylose / Q000032:analysis
chemical_list: D002241:Carbohydrates; D010938:Plant Oils; D013227:Steam; D014867:Water; D000073878:Palm Oil; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Enzymatic hydrolysis; Hot compressed water; Hydrothermal pretreatment; Oil palm mesocarp fiber; Wet disk milling pretreatment of biomass