title: Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity.
abstract: A versatile flow cytometric bead assay (FCBA) has been developed for the ultrasensitive detection of T4 PNK activity by integrating the advantages of the phosphorylation-induced hybridization chain reaction (HCR) for fluorescence signal amplification and flow cytometry for the robust, sensitive and rapid signal readout of the microbeads (MBs).
authors: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors: Yuecheng
authors: Y
authors: Zhang
authors: Chenghui
authors: C
authors: Liu
authors: Sujuan
authors: S
authors: Sun
authors: Yanli
authors: Y
authors: Tang
authors: Zhengping
authors: Z
authors: Li
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D001681:Biological Assay; D001710:Biotin / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods; D005453:Fluorescence; D008863:Microspheres; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010766:Phosphorylation; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D001710:Biotin; D019809:Streptavidin; D011116:Polynucleotide 5'-Hydroxyl-Kinase