title: Glucagon-like peptide-1 is specifically involved in sweet taste transmission.
abstract: Five fundamental taste qualities (sweet, bitter, salty, sour, umami) are sensed by dedicated taste cells (TCs) that relay quality information to gustatory nerve fibers. In peripheral taste signaling pathways, ATP has been identified as a functional neurotransmitter, but it remains to be determined how specificity of different taste qualities is maintained across synapses. Recent studies demonstrated that some gut peptides are released from taste buds by prolonged application of particular taste stimuli, suggesting their potential involvement in taste information coding. In this study, we focused on the function of glucagon-like peptide-1 (GLP-1) in initial responses to taste stimulation. GLP-1 receptor (GLP-1R) null mice had reduced neural and behavioral responses specifically to sweet compounds compared to wild-type (WT) mice. Some sweet responsive TCs expressed GLP-1 and its receptors were expressed in gustatory neurons. GLP-1 was released immediately from taste bud cells in response to sweet compounds but not to other taste stimuli. Intravenous administration of GLP-1 elicited transient responses in a subset of sweet-sensitive gustatory nerve fibers but did not affect other types of fibers, and this response was suppressed by pre-administration of the GLP-1R antagonist Exendin-4(3-39). Thus GLP-1 may be involved in normal sweet taste signal transmission in mice.
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Shingo
authors: S
authors: Takai
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Keiko
authors: K
authors: Yasumatsu
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Mayuko
authors: M
authors: Inoue
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Shusuke
authors: S
authors: Iwata
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Ryusuke
authors: R
authors: Yoshida
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Noriatsu
authors: N
authors: Shigemura
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Yuchio
authors: Y
authors: Yanagawa
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Daniel J
authors: DJ
authors: Drucker
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA.
authors: Robert F
authors: RF
authors: Margolskee
authors: *Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan; Division of Sensory Physiology, Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka, Japan; Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, and JST, CREST, Maebashi, Japan; The Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, University of Toronto, Toronto, Ontario, Canada; and Monell Chemical Senses Center, Philadelphia, Pennsylvania, USA ninomoiya.yuzo.285@m.kyushu-u.ac.jp.
authors: Yuzo
authors: Y
authors: Ninomiya
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002814:Chorda Tympani Nerve / Q000187:drug effects / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D000077270:Exenatide; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D000067757:Glucagon-Like Peptide-1 Receptor; D006851:Hydrochloric Acid / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010455:Peptides / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D018027:Receptors, Glucagon / Q000172:deficiency / Q000235:genetics; D012439:Saccharin / Q000494:pharmacology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013649:Taste; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D014688:Venoms / Q000494:pharmacology
chemical_list: C000593834:Glp1r protein, mouse; D000067757:Glucagon-Like Peptide-1 Receptor; D010455:Peptides; D018027:Receptors, Glucagon; D014688:Venoms; D012965:Sodium Chloride; D013395:Sucrose; D000584:Amiloride; D052216:Glucagon-Like Peptide 1; D000077270:Exenatide; D011803:Quinine; D012439:Saccharin; D006851:Hydrochloric Acid
keywords: gustation; incretin; transmitter