title: Simultaneous analysis of hydrodynamic and optical properties using analytical ultracentrifugation equipped with multiwavelength detection.
abstract: Analytical ultracentrifugation (AUC) has proven to be a powerful tool for the study of particle size distributions, particle shapes, and interactions with high accuracy and unrevealed resolution. In this work we show how the analysis of sedimentation velocity data from the AUC equipped with a multiwavelength detector (MWL) can be used to gain an even deeper understanding of colloidal and macromolecular mixtures. New data evaluation routines have been integrated in the software SEDANAL to allow for the handling of MWL data. This opens up a variety of new possibilities because spectroscopic information becomes available for individual components in mixtures at the same time using MWL-AUC. For systems of known optical properties information on the hydrodynamic properties of the individual components in a mixture becomes accessible. For the first time, the determination of individual extinction spectra of components in mixtures is demonstrated via MWL evaluation of sedimentation velocity data. In our paper we first provide the informational background for the data analysis and expose the accessible parameters of our methodology. We further demonstrate the data evaluation by means of simulated data. Finally, we give two examples which are highly relevant in the field of nanotechnology using colored silica and gold nanoparticles of different size and extinction properties.
authors: †Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Institute of Particle Technology (LFG), Cauerstrasse 4, 91058 Erlangen, Bavaria, Germany.
authors: Johannes
authors: J
authors: Walter
authors: ‡Interactive Technology Inc., P.O. Box 2768, Oakland, California 94602, United States.
authors: Peter J
authors: PJ
authors: Sherwood
authors: †Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Institute of Particle Technology (LFG), Cauerstrasse 4, 91058 Erlangen, Bavaria, Germany.
authors: Wei
authors: W
authors: Lin
authors: †Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Institute of Particle Technology (LFG), Cauerstrasse 4, 91058 Erlangen, Bavaria, Germany.
authors: Doris
authors: D
authors: Segets
authors: §Boston Biomedical Research Institute, c/o 12 Francis Avenue, Cambridge, Massachusetts 02138, United States.
authors: Walter F
authors: WF
authors: Stafford
authors: †Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Institute of Particle Technology (LFG), Cauerstrasse 4, 91058 Erlangen, Bavaria, Germany.
authors: Wolfgang
authors: W
authors: Peukert
mesh_terms: D006046:Gold / Q000737:chemistry; D057446:Hydrodynamics; D053768:Metal Nanoparticles / Q000737:chemistry; D055100:Optical Fibers; D055620:Optical Phenomena; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods*
chemical_list: D006046:Gold; D012822:Silicon Dioxide