title: Evaluation of a PfHRP-2 based rapid diagnostic test versus microscopy method among HIV-positive and unknown serology patients in Ouagadougou, Burkina Faso.
abstract: We evaluated the performance of a malaria rapid diagnostic test (RDT; Malaria Quick Test(®); Cypress Diagnostic) compared with the standard thick-smear microscopy method using blood samples from human immunodeficiency virus (HIV)-infected individuals and individuals of unknown HIV status collected in Ouagadougou, Burkina Faso. Our results show that 42.1% of 114 HIV-infected patients were concordantly RDT- and thick smear-positive, and 55.3% were concordantly negative. Sensitivity and specificity of the RDT test were 100.0% and 95.4%, respectively, with 5.9% false-positive results and a total agreement of 97.4%; 127 patients with unknown HIV serology were analyzed; of them, 40.9% were RDT- and thick smear-positive, and 46.4% concordantly negative. Sensitivity and specificity were 100.0% and 78.6%, respectively, with 23.5% false-positive results and a total agreement of 87.4%. Malaria Quick Test(®) is rapid and effective for the diagnosis of malaria and has a high sensitivity, confirming its use in general and HIV patients in particular.
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Arianna
authors: A
authors: Andreoli
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Pier Francesco
authors: PF
authors: Giorgetti
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Virginio
authors: V
authors: Pietra
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Alessia
authors: A
authors: Melzani
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Wetien
authors: W
authors: Seni
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso francesco.castelli@unibs.it.
authors: Francesco
authors: F
authors: Castelli
authors: Medicus Mundi Italia, Brescia, Italy; University Division of Infectious and Tropical Diseases, University of Brescia, Brescia, Italy; Centre de Recherche Biomoléculaire Pietro Annigoni, Saint Camille (CERBA), University of Ouagadougou, Ouagadougou, Burkina Faso.
authors: Jaques
authors: J
authors: Simpore
mesh_terms: D000293:Adolescent; D000328:Adult; D000953:Antigens, Protozoan / Q000097:blood*; D002050:Burkina Faso; D002648:Child; D002675:Child, Preschool; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006679:HIV Seropositivity; D006801:Humans; D016778:Malaria, Falciparum / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology* / Q000302:isolation & purification; D011237:Predictive Value of Tests; D015800:Protozoan Proteins / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D015800:Protozoan Proteins; D011933:Reagent Kits, Diagnostic