title: Synthetic long peptide booster immunization in rhesus macaques primed with replication-competent NYVAC-C-KC induces a balanced CD4/CD8 T-cell and antibody response against the conserved regions of HIV-1.
abstract: The Thai trial (RV144) indicates that a prime-boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a pox-vector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFNγ T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFNγ ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.
authors: Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
authors: Petra
authors: P
authors: Mooij
authors: Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
authors: Gerrit
authors: G
authors: Koopman
authors: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
authors: Jan Wouter
authors: JW
authors: Drijfhout
authors: Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
authors: Ivonne G
authors: IG
authors: Nieuwenhuis
authors: Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
authors: Niels
authors: N
authors: Beenhakker
authors: University of Regensburg, Franz-Josef-Strauss Allee 11, D93053 Regensburg, Germany.
authors: Josef
authors: J
authors: Koestler
authors: Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
authors: Willy M J M
authors: WMJM
authors: Bogers
authors: University of Regensburg, Franz-Josef-Strauss Allee 11, D93053 Regensburg, Germany.
authors: Ralf
authors: R
authors: Wagner
authors: Centro Nacional de Biotecnologia, CSIC, Madrid, Spain.
authors: Mariano
authors: M
authors: Esteban
authors: Swiss Vaccine Research Institute, Lausanne, Switzerland.|Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors: Giuseppe
authors: G
authors: Pantaleo
authors: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
authors: Jonathan L
authors: JL
authors: Heeney
authors: Arizona State University, Tempe, Arizona, USA.
authors: Bertram L
authors: BL
authors: Jacobs
authors: ISA pharmaceuticals, J.H. Oortweg 19-21, 2333 CH Leiden, The Netherlands.|Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
authors: Cornelis J M
authors: CJM
authors: Melief
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015497:HIV-1 / Q000276:immunology*; D007117:Immunization, Secondary / Q000379:methods*; D008253:Macaca mulatta; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D014614:Vaccines, Synthetic