title: Chlorogenic acid suppresses interleukin-1β-induced inflammatory mediators in human chondrocytes.
abstract: We investigated the anti-inflammatory properties of chlorogenic acid (CGA) in interleukin-1β-induced chondrocytes. The nitric oxide (NO) and prostaglandin E2 (PGE2) were detected by Griess and Enzyme-linked immunosorbent assay (ELISA) respectively. Quantitative real-time PCR and western blot were performed to measure the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Our results indicate that CGA inhibited the production of NO and PGE2 as well as the expression of iNOS and COX-2 in chondrocytes. Our data suggest that CGA possess potential value in the treatment of OA.
authors: Department of Orthopedics Surgery, The Second Affiliated Hospital of Medical College, Zhejiang University Hangzhou, China.
authors: Wei-Ping
authors: WP
authors: Chen
authors: Department of Orthopedics Surgery, The Second Affiliated Hospital of Medical College, Zhejiang University Hangzhou, China.
authors: Li-Dong
authors: LD
authors: Wu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology*; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D002726:Chlorogenic Acid
keywords: Chlorogenic acid; chondrocyte; cyclooxygenase; inducible NO synthase