title: Chemometric-assisted spectrophotometric methods and high performance liquid chromatography for simultaneous determination of seven β-blockers in their pharmaceutical products: a comparative study.
abstract: Chemometric-assisted spectrophotometric methods and high performance liquid chromatography (HPLC) were developed for the simultaneous determination of the seven most commonly prescribed β-blockers (atenolol, sotalol, metoprolol, bisoprolol, propranolol, carvedilol and nebivolol). Principal component regression PCR, partial least square PLS and PLS with previous wavelength selection by genetic algorithm (GA-PLS) were used for chemometric analysis of spectral data of these drugs. The compositions of the mixtures used in the calibration set were varied to cover the linearity ranges 0.7-10 μg ml(-1) for AT, 1-15 μg ml(-1) for ST, 1-15 μg ml(-1) for MT, 0.3-5 μg ml(-1) for BS, 0.1-3 μg ml(-1) for PR, 0.1-3 μg ml(-1) for CV and 0.7-5 μg ml(-1) for NB. The analytical performances of these chemometric methods were characterized by relative prediction errors and were compared with each other. GA-PLS showed superiority over the other applied multivariate methods due to the wavelength selection. A new gradient HPLC method had been developed using statistical experimental design. Optimum conditions of separation were determined with the aid of central composite design. The developed HPLC method was found to be linear in the range of 0.2-20 μg ml(-1) for AT, 0.2-20 μg ml(-1) for ST, 0.1-15 μg ml(-1) for MT, 0.1-15 μg ml(-1) for BS, 0.1-13 μg ml(-1) for PR, 0.1-13 μg ml(-1) for CV and 0.4-20 μg ml(-1) for NB. No significant difference between the results of the proposed GA-PLS and HPLC methods with respect to accuracy and precision. The proposed analytical methods did not show any interference of the excipients when applied to pharmaceutical products.
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
authors: Eman A
authors: EA
authors: Abdel Hameed
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
authors: Randa A
authors: RA
authors: Abdel Salam
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. Electronic address: ghhadad@hotmail.com.
authors: Ghada M
authors: GM
authors: Hadad
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D000465:Algorithms; D000704:Analysis of Variance; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016018:Least-Squares Analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013223:Statistics as Topic
chemical_list: D000319:Adrenergic beta-Antagonists; D004364:Pharmaceutical Preparations
keywords: GA-PLS; HPLC; PCR; PLS; Statistical experimental design; β-Blockers