title: P2Y(1) receptor mediated neuronal fibre outgrowth in organotypic brain slice co-cultures.
abstract: Extracellular purines have multiple functional roles in development, plastic remodelling, and regeneration of the CNS by stimulating certain P2X/Y receptor (R) subtypes. In the present study we elucidated the involvement of P2YRs in neuronal fibre outgrowth in the developing nervous system. We particularly focused on the P2Y1R subtype and the dopaminergic system, respectively. For this purpose, we used organotypic slice co-cultures consisting of the ventral tegmental area/substantia nigra (VTA/SN) and the prefrontal cortex (PFC). After detecting the presence of the P2Y1R in VTA/SN, PFC, and on outgrowing fibres in the border region (e.g. on glial processes) connecting both brain slices, we could show that pharmacological modulation of the receptor influenced neuronal fibre outgrowth. Biocytin-tracing and tyrosine hydroxylase-immunolabelling together with quantitative image analysis revealed a significant increase in fibre growth in the border region of the co-cultures after treatment with ADPβS (P2Y1,12,13R agonist). The observed stimulatory potential of ADPβS was inhibited by pre-treatment with the P2X/YR antagonist PPADS. In P2Y1R knockout (P2Y1R(-/-)) mice, the ADPβS-induced stimulatory effect was absent, while growth was significantly enhanced in the co-cultures of the respective wild-type. This observation was confirmed in entorhino-hippocampal co-cultures, an example of a different projection system, expressing the P2Y1R. Using wortmannin and PD98059 we further showed that PI3K/Akt and MAPK/ERK cascades are involved in the mechanism underlying ADPβS-induced fibre growth. In conclusion, the data of this study clearly indicate that activation of the P2Y1R stimulates fibre growth and thereby emphasises the general role of this particular receptor subtype during development and regeneration.
authors: Translational Centre for Regenerative Medicine (TRM), University of Leipzig, Philipp-Rosenthal-Straße 55, 04103 Leipzig, Germany; Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany. Electronic address: claudia.heine@trm.uni-leipzig.de.
authors: Claudia
authors: C
authors: Heine
authors: Translational Centre for Regenerative Medicine (TRM), University of Leipzig, Philipp-Rosenthal-Straße 55, 04103 Leipzig, Germany; Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany. Electronic address: katja.sygnecka@trm.uni-leipzig.de.
authors: Katja
authors: K
authors: Sygnecka
authors: Institute for Medical Informatics and Biometry (IMB), Dresden University of Technology, Fetscherstraße 74, 01307 Dresden, Germany. Electronic address: nico.scherf@tu-dresden.de.
authors: Nico
authors: N
authors: Scherf
authors: Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany. Electronic address: marcus.grohmann@outlook.com.
authors: Marcus
authors: M
authors: Grohmann
authors: Centre for Biotechnology and Biomedicine (BBZ), Molecular Biological-Biochemical Processing Technology, Deutscher Platz 5, 04103 Leipzig, Germany. Electronic address: annettwegner@googlemail.com.
authors: Annett
authors: A
authors: Bräsigk
authors: Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany. Electronic address: heike.franke@medizin.uni-leipzig.de.
authors: Heike
authors: H
authors: Franke
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000187:drug effects / Q000502:physiology; D018920:Coculture Techniques; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D020935:MAP Kinase Signaling System / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D017397:Prefrontal Cortex / Q000166:cytology*; D058912:Purinergic P2Y Receptor Agonists / Q000494:pharmacology; D058921:Purinergic P2Y Receptor Antagonists / Q000494:pharmacology; D051381:Rats; D058472:Receptors, Purinergic P2Y1 / Q000235:genetics / Q000378:metabolism*; D013378:Substantia Nigra / Q000166:cytology; D013873:Thionucleotides / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D017557:Ventral Tegmental Area / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D058912:Purinergic P2Y Receptor Agonists; D058921:Purinergic P2Y Receptor Antagonists; D058472:Receptors, Purinergic P2Y1; D013873:Thionucleotides; C030812:adenosine 5'-O-(2-thiodiphosphate); D005680:gamma-Aminobutyric Acid; D000244:Adenosine Diphosphate; D014446:Tyrosine 3-Monooxygenase
keywords: Dopaminergic system; ERK1/2; Fiber growth; Organotypic slice co-culture; P2Y(1) receptors; PI3K/Akt; Purinergic stimulation