title: Prognostic value of ERCC1 mRNA expression in non-small cell lung cancer, breast cancer, and gastric cancer in patients from Southern China.
abstract: BACKGROUND
  Excision repair cross complementation group 1 (ERCC1) is a nucleotide excision repair pathway gene which provides protection against platinum-based chemotherapy-induced DNA damage.
  
  
  METHODS
  ERCC1 mRNA expression was quantified by quantitative real-time reverse-transcription PCR in paraffin-embedded non-small cell lung cancer (NSCLC; n = 357), gastric cancer (n = 106), and breast cancer (n = 363) tissues. Survival curves were generated by Kaplan-Meier analysis; Cox proportional multivariate regression analysis was applied.
  
  
  RESULTS
  ERCC1 mRNA expression was significantly higher in breast cancer than gastric cancer or NSCLC (both P < 0.0001), but not significantly different in NSCLC and gastric cancer (P = 0.119). In NSCLC, the low ERCC1 group had significantly longer disease free survival (DFS) than the high ERCC1 group (29.1 vs. 21.0 months, P < 0.0001); in the surgery alone and postoperative platinum-containing chemotherapy subgroups, DFS was significantly longer for the low ERCC1 groups than high ERCC1 groups (30.2 vs. 25.1 months, P = 0.018; 27.0 vs. 19.4 months, P < 0.0001, respectively). In gastric cancer patients receiving surgery alone, the low ERCC1 group had significantly longer overall survival than the high ERCC1 group (47.54 vs. 27.47 months, P = 0.018).
  
  
  CONCLUSIONS
  High ERCC1 mRNA expression of the NSCLC tumor tissues was associated with poor disease-free survival (DFS), in both the surgery alone and postoperative platinum-containing chemotherapy subgroups. Meanwhile, low ERCC1 mRNA expression had significantly longer overall survival in gastric cancer patients receiving surgery alone. Therefore, ERCC1 expression was a prognostic factor and predictive marker in NSCLC, and gastric cancer after surgery alone, but was not a prognostic factor in breast cancer.
authors: Center for Translational Medicine, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Qiuhua
authors: Q
authors: Deng
authors: State Key Laboratory of Respiratory Diseases Guangzhou, China ; Cardiothoracic Surgery, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Haihong
authors: H
authors: Yang
authors: Department of Medical Laboratory, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Yongping
authors: Y
authors: Lin
authors: State Key Laboratory of Respiratory Diseases Guangzhou, China ; Cardiothoracic Surgery, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Yuan
authors: Y
authors: Qiu
authors: Department of Pathology, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Xia
authors: X
authors: Gu
authors: Department of Pathology, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Ping
authors: P
authors: He
authors: State Key Laboratory of Respiratory Diseases Guangzhou, China ; Cardiothoracic Surgery, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Meiling
authors: M
authors: Zhao
authors: Department of Medical Laboratory, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Hui
authors: H
authors: Wang
authors: Department of Medical Laboratory, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Yunjian
authors: Y
authors: Xu
authors: Department of Pathology, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Yunen
authors: Y
authors: Lin
authors: Department of Pathology, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Juhong
authors: J
authors: Jiang
authors: State Key Laboratory of Respiratory Diseases Guangzhou, China ; Cardiothoracic Surgery, The First Affiliated Hospital, Guangzhou Medical University Guangzhou, China.
authors: Jianxing
authors: J
authors: He
authors: Ningbo University School of Medicine Ningbo, China.
authors: Jeff X
authors: JX
authors: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality; D002681:China; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D018572:Disease-Free Survival; D004720:Endonucleases / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C071447:ERCC1 protein, human; D004720:Endonucleases
keywords: ERCC1; NSCLC; breast cancer; expression; gastric cancer; mRNA; prognostic factor