title: Optimization of microwave-assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill.
abstract: A microwave-assisted enzymatic extraction (MAEE) method had been developed, which was optimized by response surface methodology (RSM) and orthogonal test design, to enhance the extraction of crude polysaccharides (CPS) from the fruit of Schisandra chinensis Baill. The optimum conditions were as follows: microwave irradiation time of 10 min, extraction pH of 4.21, extraction temperature of 47.58°C, extraction time of 3h and enzyme concentration of 1.5% (wt% of S. chinensis powder) for cellulase, papain and pectinase, respectively. Under these conditions, the extraction yield of CPS was 7.38 ± 0.21%, which was well in close agreement with the value predicted by the model. The three methods including heat-refluxing extraction (HRE), ultrasonic-assisted extraction (UAE) and enzyme-assisted extraction (EAE) for extracting CPS by RSM were further compared. Results indicated MAEE method had the highest extraction yields of CPS at lower temperature. It was indicated that the proposed approach in this study was a simple and efficient technique for extraction of CPS in S. chinensis Baill.
authors: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors: Zhenyu
authors: Z
authors: Cheng
authors: Laboratory of Test Center, Jilin Agricultural Science and Technology College, Jilin 132101, China.
authors: Haiyan
authors: H
authors: Song
authors: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China. Electronic address: yanghjm@163.com.
authors: Yingjie
authors: Y
authors: Yang
authors: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors: Yan
authors: Y
authors: Liu
authors: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors: Zhigang
authors: Z
authors: Liu
authors: College of Cemistry and Chemical Engineering, Longdong University, Qingyang 745000, China.
authors: Haobin
authors: H
authors: Hu
authors: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors: Yang
authors: Y
authors: Zhang
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D008872:Microwaves; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000648:ultrastructure; D032141:Schisandra / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides
keywords: Microwave-assisted enzymatic extraction; Optimization; Polysaccharide; Response surface methodology; Schisandra chinensis Baill