title: The molecular timeline of a reviving bacterial spore.
abstract: The bacterial spore can rapidly convert from a dormant to a fully active cell. Here we study this remarkable cellular transition in Bacillus subtilis and reveal the identity of the newly synthesized proteins throughout spore revival. Our analysis uncovers a highly ordered developmental program that correlates with the spore morphological changes and reveals the spatial and temporal molecular events fundamental to reconstruct a cell. As opposed to current knowledge, we found that translation takes place during the earliest revival event, termed germination, a process hitherto considered to occur without the need for any macromolecule synthesis. Furthermore, we demonstrate that translation is required for execution of germination and relies on the bona fide translational factors RpmE and Tig. Our study sheds light on the spore revival process and on the vital building blocks underlying cellular awakening, thereby paving the way for designing new antimicrobial agents to eradicate spore-forming pathogens.
authors: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, POB 12272, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel.
authors: Lior
authors: L
authors: Sinai
authors: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, POB 12272, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel.
authors: Alex
authors: A
authors: Rosenberg
authors: Genomic Data Analysis Unit, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel.
authors: Yoav
authors: Y
authors: Smith
authors: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, POB 12272, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel.
authors: Einat
authors: E
authors: Segev
authors: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, POB 12272, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel. Electronic address: sigalb@ekmd.huji.ac.il.
authors: Sigal
authors: S
authors: Ben-Yehuda
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D016764:Cell Polarity; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological; D014176:Protein Biosynthesis; D020543:Proteome; D013171:Spores, Bacterial / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D020543:Proteome