title: NIRF, a Novel Ubiquitin Ligase, Inhibits Hepatitis B Virus Replication Through Effect on HBV Core Protein and H3 Histones.
abstract: Np95/ICBP90-like RING finger protein (NIRF), a novel E3 ubiquitin ligase, has been shown to interact with HBc and promote its degradation. This study investigated the effects of NIRF on replication of hepatitis B virus (HBV) and the mechanisms. We have shown that NIRF inhibits replication of HBV DNA and secretion of HBsAg and HBeAg in HepG2 cells transfected with pAAV-HBV1.3. NIRF also inhibits the replication and secretion of HBV in a mouse model that expressed HBV. NIRF reduces acetylation of HBV cccDNA-bound H3 histones. These results showed that NIRF is involved in the HBV replication cycle not only through direct interaction with HBc but also reduces acetylation of HBV cccDNA-bound H3 histones.
authors: 1 Department of Cell Biology and Medical Genetics, Chongqing Medical University , Chongqing, China .
authors: Guanhua
authors: G
authors: Qian
authors: Bin
authors: B
authors: Hu
authors: Danlin
authors: D
authors: Zhou
authors: Yanyan
authors: Y
authors: Xuan
authors: Lu
authors: L
authors: Bai
authors: Changzhu
authors: C
authors: Duan
mesh_terms: D000107:Acetylation; D000818:Animals; D015153:Blotting, Western; D004279:DNA, Viral / Q000235:genetics; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000235:genetics / Q000378:metabolism*; D006515:Hepatitis B virus / Q000502:physiology*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D006657:Histones; D012333:RNA, Messenger; D025801:Ubiquitin; C465619:UHRF2 protein, human; D044767:Ubiquitin-Protein Ligases