title: Impact of vascular endothelial growth factor single nucleotide polymorphism association on acute renal allograft rejection.
abstract: BACKGROUND
  Vascular endothelial growth factor (VEGF) is a cytokine which plays an important role in the division, proliferation and migration of endothelial cells. In the kidney, VEGF expression is found in glomerular podocytes and in tubular epithelial cells, which may result in acute inflammatory reactions.
  
  
  METHODS
  The role of VEGF gene polymorphisms (-2578 C/A, -2549 18 bp Ins/Del, -1154 G/A and +936 C/T) was investigated in 272 patients who underwent renal transplantation. ARMS-PCR and PCR-RFLP were used. Patients were categorized into acute allograft rejection (n = 76) and nonrejection (n = 196).
  
  
  RESULTS
  The VEGF -1154 GG genotype and the +936 T allele were found to be susceptible to acute rejection (AR). T-A-A-I, T-A-A-D, T-G-C-I and C-A-A-I haplotypes revealed a predisposition among AR cases. In silico analysis revealed +936 T as a significant allele involved in the transcription regulation.
  
  
  CONCLUSIONS
  These results highlight the role of VEGF polymorphisms in acute allograft rejection.
authors: Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
authors: Swayam
authors: S
authors: Prakash
authors: Suraksha
authors: S
authors: Agrawal
authors: Shashi
authors: S
authors: Kumar
authors: Narayan
authors: N
authors: Prasad
mesh_terms: D000208:Acute Disease; D003198:Computer Simulation; D004247:DNA / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D016030:Kidney Transplantation; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D004247:DNA