title: Structural characterization and anticoagulant activity of a sulfated polysaccharide from the green alga Codium divaricatum.
abstract: A sulfated polysaccharide, designated CP2-1, was isolated from the green alga Codium divaricatum by water extraction and purified by anion-exchange and size-exclusion chromatography. CP2-1 is a galactan which is highly sulfated and substituted with pyruvic acid ketals. On the basis of chemical and spectroscopic analyses, the backbone of CP2-1 was mainly composed of (1→3)-β-d-galactopyranose residues, branched by single (1→)-β-d-galactopyranose units attached to the main chain at C-4 positions. The degree of branching was estimated to be about 12.2%. Sulfate groups were at C-4 of (1→3)-β-d-galactopyranose and C-6 of non-reducing terminal galactose residues. In addition, the ketals of pyruvic acid were found at 3,4- of non-reducing terminal galactose residues forming a five-membered ring. CP2-1 possessed a high anticoagulant activity as assessed by the activated partial thromboplastin time and thrombin time assays. The investigation demonstrated that CP2-1 was an anticoagulant-active sulfated polysaccharide distinguishing from other sulfated polysaccharides from marine green algae.
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Na
authors: N
authors: Li
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China. Electronic address: wenjunmqd@hotmail.com.
authors: Wenjun
authors: W
authors: Mao
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Mengxia
authors: M
authors: Yan
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Xue
authors: X
authors: Liu
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Zheng
authors: Z
authors: Xia
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Shuyao
authors: S
authors: Wang
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Bo
authors: B
authors: Xiao
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Chenglong
authors: C
authors: Chen
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Lifang
authors: L
authors: Zhang
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Sujian
authors: S
authors: Cao
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D000460:Chlorophyta / Q000737:chemistry*; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry*; D013918:Thrombin Time; D014867:Water / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates; D014867:Water
keywords: Anticoagulant activity; Codium divaricatum; Marine green alga; Structural characterization; Sulfated polysaccharide