title: (1)H, (13)C and (15)N backbone and side-chain resonance assignment of the LAM-RRM1 N-terminal module of La protein from Dictyostelium discoideum.
abstract: The N-terminal half of La protein consists of two concatenated motifs: La motif (LAM) and the N-terminal RNA recognition motif (RRM1) both of which are responsible for poly(U) RNA binding. Here, we present the backbone and side-chain assignments of the (1)H, (13)C and (15)N resonances of the 191-residue LAM-RRM1 region of the La protein from the lower eukaryote Dictyostelium discoideum and its secondary structure prediction.
authors: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors: Christos T
authors: CT
authors: Chasapis
authors: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors: Aikaterini I
authors: AI
authors: Argyriou
authors: Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece.
authors: Maria
authors: M
authors: Apostolidi
authors: Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece.
authors: Parthena
authors: P
authors: Konstantinidou
authors: Institute of Physiology II, University of Freiburg, 79104, Freiburg, Germany.
authors: Detlef
authors: D
authors: Bentrop
authors: Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece.
authors: Constantinos
authors: C
authors: Stathopoulos
authors: Department of Pharmacy, University of Patras, 26504, Patras, Greece. G.A.Spyroulias@upatras.gr.
authors: Georgios A
authors: GA
authors: Spyroulias
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004023:Dictyostelium / Q000378:metabolism*; D009587:Nitrogen Isotopes; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D015800:Protozoan Proteins / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; D015800:Protozoan Proteins
keywords: La protein; NMR spectroscopy; RNA binding; RNA polymerase III; Recombinant protein expression