title: Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance.
abstract: Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.
authors: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331, USA Research and Development, Medolac Laboratories, 6305 SW Rosewood St, Suite B, Lake Oswego, OR 97035, USA.
authors: Jun
authors: J
authors: Ding
authors: Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA.
authors: Garrett
authors: G
authors: Holzwarth
authors: Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331, USA.
authors: Michael H
authors: MH
authors: Penner
authors: Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.
authors: Jana
authors: J
authors: Patton-Vogt
authors: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331, USA alan.bakalinsky@oregonstate.edu.
authors: Alan T
authors: AT
authors: Bakalinsky
mesh_terms: D000106:Acetate-CoA Ligase / Q000235:genetics* / Q000378:metabolism*; D019342:Acetic Acid / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*
chemical_list: D000105:Acetyl Coenzyme A; D000106:Acetate-CoA Ligase; D019342:Acetic Acid
keywords: Acs2; Saccharomyces cerevisiae; acetic acid; acetyl-CoA synthetase; lignocellulose