title: Clinical utility comparison of two benchtop deep sequencing instruments for rapid diagnosis of newly emergent influenza infections.
abstract: Rapid determination of full genomes of influenza A viruses from clinical samples is vital to outbreak preparedness. We benchmarked Ion Torrent PGM and Illumina MiSeq to the standard Sanger sequencing on two real clinical samples and demonstrated their performance for full-genome determination and polymorphism identification.
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: X
authors: X
authors: Ren
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: Y
authors: Y
authors: Hu
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: F
authors: F
authors: Yang
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: Y
authors: Y
authors: Xiao
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: L
authors: L
authors: Li
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: J
authors: J
authors: Dong
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: L
authors: L
authors: Sun
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: Y
authors: Y
authors: Zhu
authors: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address: zdsys@vip.sina.com.
authors: Q
authors: Q
authors: Jin
mesh_terms: D016679:Genome, Viral; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000235:genetics*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
keywords: Deep sequencing; Ion Torrent PGM; MiSeq; genome sequencing; influenza