title: Identification of differentially expressed miRNAs in mouse spinal cord development.
abstract: MicroRNAs (miRNAs) are a class of non-coding, regulatory small RNAs of ∼22 nt. It was implicated that these small RNAs play critical roles in various important biological processes. During development, some miRNAs are specifically expressed in individual tissues and at particular developmental stages. Many miRNAs show distinct expression patterns in the development of central nervous system, including spinal cord. In this study, we first reported the miRNAs expression in the development of mouse spinal cord. Differentially expressed miRNAs in embryonic (day 13.5) and neonatal mice spinal cords were identified. The predicted target genes of the differentially expressed miRNAs were subject to gene ontology and KEGG pathway analysis, and several nervous development-related pathways were enriched, implying that these miRNAs may be involved in these pathways that regulate mouse spinal cord development.
authors: School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors: Botao
authors: B
authors: Zhao
authors: Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: Menggui
authors: M
authors: Huang
authors: Department of Neurology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin 214400, China.
authors: Yanyan
authors: Y
authors: Bai
authors: Department of Etiology, Qidong Liver Cancer Institute, Qidong 226200, China.
authors: Chunsun
authors: C
authors: Fan
authors: Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA yxjin@sibs.ac.cn yi.fan@uphs.upenn.edu.
authors: Yi
authors: Y
authors: Fan
authors: School of Life Sciences, Shanghai University, Shanghai 200444, China State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China yxjin@sibs.ac.cn yi.fan@uphs.upenn.edu.
authors: Youxin
authors: Y
authors: Jin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015536:Down-Regulation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs
keywords: miRNA; miRNA microarray; spinal cord development