title: Identification of five Shiga toxin-producing Escherichia coli genes by Luminex microbead-based suspension array.
abstract: To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors (stx1, stx2, eae, and ehxA) plus the O157:H7-specific +93 uidA single nucleotide polymorphism.
authors: U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: insook.son@fda.hhs.gov.
authors: Insook
authors: I
authors: Son
authors: U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: rachel.binet@fda.hhs.gov.
authors: Rachel
authors: R
authors: Binet
authors: U.S. Food and Drug Administration, San Francisco Laboratory, Alameda, CA 94502, USA. Electronic address: andrew.lin@fda.hhs.gov.
authors: Andrew
authors: A
authors: Lin
authors: U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: thomas.hammack@fda.hhs.gov.
authors: Thomas S
authors: TS
authors: Hammack
authors: U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: julie.kase@fda.hhs.gov.
authors: Julie A
authors: JA
authors: Kase
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D008863:Microspheres; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors; C073819:eaeA protein, E coli