title: Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103.
abstract: The PhlG protein from Mycobacterium abscessus 103 (mPhlG), which shares 30% sequence identity with phloretin hydrolase from Eubacterium ramulus and 38% sequence identity with 2,4-diacetylphloroglucinol hydrolase from Pseudomonas fluorescens Pf-5, is a putative carbon-carbon bond hydrolase. Here, the expression, purification and crystallization of mPhlG are reported. Crystals were obtained using a precipitant consisting of 100 mM citric acid pH 5.0, 1.0 M lithium chloride, 8%(w/v) polyethylene glycol 6000. The crystals diffracted to 1.87 Å resolution and belonged to space group P21, with unit-cell parameters a = 71.0, b = 63.4, c = 74.7 Å, α = 90.0, β = 103.2, γ = 90.0°. Assuming the presence of two mPhlG molecules in the asymmetric unit, VM was calculated to be 2.5 Å(3) Da(-1), which corresponds to a solvent content of 50%.
authors: MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors: Zhang
authors: Z
authors: Zhang
authors: Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
authors: Yong-Liang
authors: YL
authors: Jiang
authors: MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors: Yi
authors: Y
authors: Wu
authors: MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.
authors: Yong-Xing
authors: YX
authors: He
mesh_terms: D000595:Amino Acid Sequence; D002244:Carbon / Q000737:chemistry*; D002850:Chromatography, Gel; D003460:Crystallization; D006867:Hydrolases / Q000737:chemistry*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D002244:Carbon; D006867:Hydrolases
keywords: Mycobacterium abscessus 103; PhlG; carbon–carbon bond hydrolase