title: Long-term tracing of the BrdU label-retaining cells in adult rat brain.
abstract: Stem cells have been shown to be label-retaining, slow-cycling cells. In the adult mammalian central nervous system, the distribution of the stem cells is inconsistent among previous studies. The purpose of the present study was to determine the distribution of BrdU-LRCs and the cell types of the BrdU-LRCs in rat brain. To label BrdU-LRCs in rat brain, six newborn rats were administered intraperitoneal injections of BrdU 50mg/kg/time twice a day at 2h intervals, over four consecutive days. The BrdU-LRCs were detected by immunohistochemistry, the cell types were examined by double immunofluorescence staining for BrdU/GFAP and BrdU/MAP2, and the percentage of BrdU-LRCs was calculated following a chase period of 24 weeks post-injection. We observed that BrdU-LRCs distributed extensively in rat brain. In the LV, DG, striatum, cerebellum and neocortex, the percentage of BrdU-LRCs was 11.3 ± 2.5%, 10.9 ± 1.3%, 6.4 ± 1.2%, 5.6 ± 0.8%, and 4.9 ± 0.6%, respectively. The highest density of BrdU-LRCs was in LV and DG, the known stem cell sites in adult mammalian brain. Both BrdU/GFAP and BrdU/MAP2 double-staining cells could be detected in the above five brain subregions. Ongoing cell production was widespread in the adult mammalian brain, which would allow us to reevaluate the capacity and potentiality of the brain in homeostasis, wound repair, and regeneration.
authors: Psychiatric and Psychological Department, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, China; Mental Health Center, Shantou University Medical College, North Taishan Road, Shantou, Guangdong Province 515065, China.
authors: Lei
authors: L
authors: Zhang
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, China. Electronic address: lihaihong1051@126.com.
authors: Haihong
authors: H
authors: Li
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, China.
authors: Shaopeng
authors: S
authors: Zeng
authors: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, China.
authors: Lu
authors: L
authors: Chen
authors: Mental Health Center, Shantou University Medical College, North Taishan Road, Shantou, Guangdong Province 515065, China.
authors: Zeman
authors: Z
authors: Fang
authors: Mental Health Center, Shantou University Medical College, North Taishan Road, Shantou, Guangdong Province 515065, China.
authors: Qingjun
authors: Q
authors: Huang
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000378:metabolism*; D007202:Indicators and Reagents; D017207:Rats, Sprague-Dawley
chemical_list: D007202:Indicators and Reagents; D001973:Bromodeoxyuridine
keywords: Brain; BrdU; LRCs; Rat; Stem cells