title: Enhanced expression of long noncoding RNA CARLo-5 is associated with the development of gastric cancer.
abstract: The identification of cancer-associated long non-coding RNAs and the investigation of their molecular and biological functions are vital for understanding the molecular biology and progression of cancer. The CARLo-5, a newly identified long non-coding RNA, was found to be upregulated in colon cancer. However, little is known about its role in gastric cancer. In the present study, a great upregulation of CARLo-5 was observed in gastric cancer compared to paired adjacent normal tissues. Knockdown of CARLo-5 in gastric cancer cell lines significantly inhibited the cell proliferation via inducing G0/G1 cell-cycle arrest and apoptosis. Furthermore, ERK/MAPK pathway was found to be inactivated in the gastric cells after CARLo-5 knockdown. These results indicated that CARLo-5 might serve as a pro-oncogenic lncRNA that promotes proliferation of gastric cancer and activates the ERK/MAPK pathway.
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University Wuhan, Hubei, China ; Department of Gastroenterology, Yijishan Hospital Affiliated to Wannan Medical College Wuhu, Anhui, China.
authors: Yan
authors: Y
authors: Zhang
authors: Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors: Mingzhe
authors: M
authors: Ma
authors: Department of Ultrasound, Anhui Province Hospital Affiliated to Anhui Medical University Hefei, Anhui, China.
authors: Weiyong
authors: W
authors: Liu
authors: Department of Radiotherapy, Yijishan Hospital Affiliated to Wannan Medical College Wuhu, Anhui, China.
authors: Wenping
authors: W
authors: Ding
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University Wuhan, Hubei, China.
authors: Honggang
authors: H
authors: Yu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C579842:CCAT1 long noncoding RNA, human; D062085:RNA, Long Noncoding
keywords: CARLo-5; ERK; MAPK; gastric cancer; lncRNA; proliferation