title: Role of endogenous PDGF-BB in cultured cardiomyocytes exposed to hypoxia.
abstract: Platelet-derived growth factor-BB (PDGF-BB) plays a critical role in cell proliferation, angiogenesis and fibrosis. However, its exact role in cardiomyocytes exposed to hypoxia is not well known. This study was therefore designed to detect whether PDGF-BB expression was changed in a hypoxic condition, then the possible role of endogenous PDGF-BB in cardiomyocytes was explored, with interference RNA in a lentiviral vector ex vivo. The results showed that cultured cardiomyocytes exhibited an optimal proliferation from 3 to 10 days. However, LDH level was significantly increased but the heart rhythm was not altered in cardiomyocytes exposed to hypoxia for 24 hours. PDGF-BB expression was substantially upregulated in hypoxic cardiomyocytes. In order to know the role of PDGF-BB, we performed PDGF-BB knockdown in cultured cardiomyocytes. The number of apoptotic cells and the level of LDH were significantly increased but the beat rhythm was reduced in cardiomyocytes with PDGF-BB knockdown. These findings suggest that endogenous PDGF-BB exerts a crucial protective effect to cultured cardiomyocytes exposed to hypoxia.
authors: Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China; Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, Anhui, China.
authors: Rong
authors: R
authors: Rong
authors: Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: You-Cui
authors: YC
authors: Wang
authors: Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, Anhui, China.
authors: Li-qun
authors: LQ
authors: Hu
authors: Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Qin-qin
authors: QQ
authors: He
authors: School of Pharmacy and Medical Sciences, Sansom Institute, Division of Health Sciences, University of South Australia, Adelaide, SA, Australia.
authors: Xin-Fu
authors: XF
authors: Zhou
authors: Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China; Institute of Neurological Disease, State Key Lab of Biotherapy, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Ting-hua
authors: TH
authors: Wang
authors: Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China. Electronic address: bupeili@medmail.com.cn.
authors: Pei-li
authors: PL
authors: Bu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D000077214:Becaplermin; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000648:ultrastructure; D020574:Proto-Oncogene Proteins c-sis / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D015854:Up-Regulation
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077214:Becaplermin; D007770:L-Lactate Dehydrogenase
keywords: Cardiomyocytes; Hypoxia; Knockdown; PDGF-BB