title: Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds.
abstract: The present study was aimed to screen genetic variation within a SNP (rs136500299) located at exon 14 region of bovine ITGB6 gene among different Zebu cattle breeds. The genotyping method describe in the present study is a tetraplex ARMS PCR, which offers extremely fast, economical, and simple detection tool. The distribution of the ITGB6 genotypes among the different breeds studied suggested that the populations were under Hardy-Weinberg equilibrium. Our findings revealed that TT genotypes are widely distributed among different Zebu cattle breeds, which can be associated with the resistance to FMD virus, as the Bos indicus are more resistant to FMD virus in comparison to Bos taurus.
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Rani
authors: R
authors: Singh
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Rajib
authors: R
authors: Deb
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Umesh
authors: U
authors: Singh
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: T V
authors: TV
authors: Raja
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Rani
authors: R
authors: Alex
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Sushil
authors: S
authors: Kumar
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Soumendu
authors: S
authors: Chakraborti
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Gyanendra
authors: G
authors: Sengar
authors: Molecular Genetics Laboratory, Central Institute for Research on Cattle (Indian Council of Agricultural Research), Meerut 250 001, Uttar Pradesh, India.
authors: Sheetal
authors: S
authors: Sharma
keywords: ARMS PCR; FMD; Zebu; rs136500299