title: Development and characterization of alginate coated low molecular weight chitosan nanoparticles as new carriers for oral vaccine delivery in mice.
abstract: In the present study, nanoparticles of low MW chitosan (CS) were formulated in which measles antigen was entrapped and subsequently coated with sodium alginate. The size and surface properties of the nanoparticle can be tuned with different MW of CS. In vitro release studies showed initial burst release followed by extended release, best fitted in the Makoid-Banakar model (R(2)>0.98). SDS-PAGE assay revealed that alginate coating could effectively protect antigen in acidic condition for at least 2h. Cell viability was assessed using MTT assay into HT 29 cell line. Formulations were orally administered to mice and immunological responses were evaluated using ELISA method. Obtained results showed that measles antigen-loaded CS nanoparticles induced strong immune response and significant correlation was observed between the immune response with CS MW. Protecting ability of antigen in gastric environment, sustained release kinetics, systemic and mucosal immune responses and low cytotoxicity observed for the alginate coated nanoparticles demonstrated that LMW CS could be promising platform for oral vaccine delivery.
authors: National Institute of Cholera and Enteric Diseases, Indian Council of Medical Research, Kolkata 700010, India. Electronic address: sai_brata@yahoo.com.
authors: Subrata
authors: S
authors: Biswas
authors: Department of Pharmacology, Bengal School of Technology, Hooghly 712102, West Bengal, India. Electronic address: mainakcool@yahoo.co.in.
authors: Mainak
authors: M
authors: Chattopadhyay
authors: Department of Pharmaceutics, Gupta College of Technological Sciences, Ashram More, G.T. Road, Asansol 713301, West Bengal, India. Electronic address: kks_kpa@yahoo.co.in.
authors: Kalyan Kumar
authors: KK
authors: Sen
authors: National Institute of Cholera and Enteric Diseases, Indian Council of Medical Research, Kolkata 700010, India. Electronic address: sahamk@yahoo.com.
authors: Malay Kumar
authors: MK
authors: Saha
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000009:adverse effects / Q000737:chemistry*; D000818:Animals; D048271:Chitosan / Q000009:adverse effects / Q000737:chemistry*; D020723:Glucuronic Acid / Q000009:adverse effects / Q000737:chemistry; D019073:HT29 Cells; D006603:Hexuronic Acids / Q000009:adverse effects / Q000737:chemistry; D006801:Humans; D008458:Measles Vaccine / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D008458:Measles Vaccine; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Adjuvant; Cytotoxicity; Mucosal immunity; Vaccine