title: The salivary proteome profile in patients affected by SAPHO syndrome characterized by a top-down RP-HPLC-ESI-MS platform.
abstract: SAPHO syndrome is a rare and often unrecognized disease with prominent inflammatory cutaneous and articular symptoms characterized by musculoskeletal manifestations (synovitis, hyperostosis, osteomyelitis) associated with dermatological conditions (severe acne and pustulosis). The acidic soluble fraction of whole saliva from 10 adult women affected by SAPHO syndrome and from a group of 28 healthy women was analysed by RP-HPLC-ESI-MS with the aim of discovering salivary biomarkers of the disorder. The levels of the oral proteins and peptides were correlated with clinical data. The following proteins showed a significant decreased concentration in saliva of SAPHO subjects with respect to controls: cystatin S1 and SN, histatins, the major acidic PRPs, P-C and P-B peptides. The cystatin SN abundance lowered according to the disease duration and histatins showed positive correlations with the C reactive protein. Statistical analysis performed excluding one patient with a different pattern of salivary proteins/peptides highlighted a positive relationship between cystatin S1, histatins 3, histatin 5, and the neutrophil count. Moreover, histatin 3 correlated positively with the total white cell count and negatively with the erythrocyte sedimentation rate. Levels and frequency of S100A12 protein showed a trend to increase in SAPHO patients. The high expression of this pro-inflammatory protein is probably related to the inflammatory response and to the altered neutrophil responses to functional stimuli that characterize SAPHO syndrome suggesting a possible application as a salivary biomarker.
authors: Department of Life and Environmental Sciences, Biomedical section, University of Cagliari, Monserrato Campus, 09042 Monserrato, CA, Italy. tcabras@unica.it.
authors: Monica
authors: M
authors: Sanna
authors: Davide
authors: D
authors: Firinu
authors: Paolo Emilio
authors: PE
authors: Manconi
authors: Maria
authors: M
authors: Pisanu
authors: Giuseppe
authors: G
authors: Murgia
authors: Valentina
authors: V
authors: Piras
authors: Massimo
authors: M
authors: Castagnola
authors: Irene
authors: I
authors: Messana
authors: Stefano Renato
authors: SR
authors: del Giacco
authors: Tiziana
authors: T
authors: Cabras
mesh_terms: D020083:Acquired Hyperostosis Syndrome / Q000378:metabolism*; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D018709:Statistics, Nonparametric
chemical_list: D020543:Proteome; D012471:Salivary Proteins and Peptides