title: In vitro anti-inflammatory activity of fractionated Euphorbia hirta aqueous extract on rabbit synovial fibroblasts.
abstract: BACKGROUND
  Euphorbia hirta has been reported to possess anti-inflammatory activity. This study was carried out to determine the prostaglandin E 2 (PGE 2 ) inhibition activity of the fractions of the E. hirta aqueous extract on rabbit synovial fibroblast cells (HIG-82).
  
  
  METHODS
  E. hirta aqueous extract was fractionated into five fractions (fractions A, B, C, D, and E) by reversed phase flash chromatography. Rabbit synovial fibroblast cells (HIG-82) were activated with phorbol myristate acetate and treated with the fractions. The amount of PGE 2 released into the medium was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Fraction A (0.1, 1, and 10 μg/ml) had the greatest PGE 2 inhibitory effect among the five fractions, and showed a greater extent of PGE 2 inhibition compared to the aqueous extract. In contrast, Fraction E had the greatest stimulatory effect on PGE 2 release.
  
  
  CONCLUSIONS
  Fraction A of the aqueous extract inhibited the production of PGE 2 from activated HIG-82 cells to a greater extent than the crude aqueous extract. Bioactive compounds with anti-inflammatory activity are likely to be concentrated in Fraction A of E. hirta aqueous extract.
authors: Jocelyn
authors: J
authors: Chen
authors: School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.
authors: Hui Meng
authors: HM
authors: Er
authors: Shar Mariam
authors: SM
authors: Mohamed
authors: Yu Sui
authors: YS
authors: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism*; D028481:Euphorbia / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D015232:Dinoprostone