title: Integrated transcriptomic and proteomic analysis of the global response of Synechococcus to high light stress.
abstract: Sufficient light is essential for the growth and physiological functions of photosynthetic organisms, but prolonged exposure to high light (HL) stress can cause cellular damage and ultimately result in the death of these organisms. Synechococcus sp. PCC 7002 (hereafter Synechococcus 7002) is a unicellular cyanobacterium with exceptional tolerance to HL intensities. However, the molecular mechanisms involved in HL response by Synechococcus 7002 are not well understood. Here, an integrated RNA sequencing transcriptomic and quantitative proteomic analysis was performed to investigate the cellular response to HL in Synechococcus 7002. A total of 526 transcripts and 233 proteins were identified to be differentially regulated under HL stress. Data analysis revealed major changes in mRNAs and proteins involved in the photosynthesis pathways, resistance to light-induced damage, DNA replication and repair, and energy metabolism. A set of differentially expressed mRNAs and proteins were validated by quantitative RT-PCR and Western blot, respectively. Twelve genes differentially regulated under HL stress were selected for knockout generation and growth analysis of these mutants led to the identification of key genes involved in the response of HL in Synechococcus 7002. Taken altogether, this study established a model for global response mechanisms to HL in Synechococcus 7002 and may be valuable for further studies addressing HL resistance in photosynthetic organisms.
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
authors: Qian
authors: Q
authors: Xiong
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; §University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Jie
authors: J
authors: Feng
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; §University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Si-ting
authors: ST
authors: Li
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; §University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Gui-ying
authors: GY
authors: Zhang
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
authors: Zhi-xian
authors: ZX
authors: Qiao
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
authors: Zhuo
authors: Z
authors: Chen
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; §University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Ying
authors: Y
authors: Wu
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
authors: Yan
authors: Y
authors: Lin
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; gefeng@ihb.ac.cn litao@ihb.ac.cn jzhao@ihb.ac.cn.
authors: Tao
authors: T
authors: Li
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; gefeng@ihb.ac.cn litao@ihb.ac.cn jzhao@ihb.ac.cn.
authors: Feng
authors: F
authors: Ge
authors: From the ‡Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; gefeng@ihb.ac.cn litao@ihb.ac.cn jzhao@ihb.ac.cn.
authors: Jin-dong
authors: JD
authors: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D055786:Gene Knockout Techniques; D063990:Gene Ontology; D008027:Light; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics / Q000528:radiation effects; D046940:Synechococcus / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects*; D020524:Thylakoids / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D059467:Transcriptome / Q000235:genetics / Q000528:radiation effects
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012333:RNA, Messenger; D017382:Reactive Oxygen Species