title: Target-specific near-IR induced drug release and photothermal therapy with accumulated Au/Ag hollow nanoshells on pulmonary cancer cell membranes.
abstract: Au/Ag hollow nanoshells (AuHNSs) were developed as multifunctional therapeutic agents for effective, targeted, photothermally induced drug delivery under near-infrared (NIR) light. AuHNSs were synthesized by galvanic replacement reaction. We further conjugated antibodies against the epidermal growth factor receptor (EGFR) to the PEGylated AuHNS, followed by loading with the antitumor drug doxorubicin (AuHNS-EGFR-DOX) for lung cancer treatment. AuHNSs showed similar photothermal efficiency to gold nanorods under optimized NIR laser power. The targeting of AuHNS-EGFR-DOX was confirmed by light-scattering images of A549 cells, and doxorubicin release from the AuHNSs was evaluated under low pH and NIR-irradiated conditions. Multifunctional AuHNS-EGFR-DOX induced photothermal ablation of the targeted lung cancer cells and rapid doxorubicin release following irradiation with NIR laser. Furthermore, we evaluated the effectiveness of AuHNS-EGFR-DOX drug delivery by comparing two drug delivery methods: receptor-mediated endocytosis and cell-surface targeting. Accumulation of the AuHNS-EGFR-DOX on the cell surfaces by targeting EGFR turned out to be more effective for lung cancer treatments than uptake of AuHNS-EGFR-DOX. Taken together, our data suggest a new and optimal method of NIR-induced drug release via the accumulation of targeted AuHNS-EGFR-DOX on cancer cell membranes.
authors: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea; Program in Nano Science and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea; Medical Supplies Evaluation Center, Korea Testing Certification, Gunpo 435-862, Republic of Korea.
authors: Mi Suk
authors: MS
authors: Noh
authors: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea; Graduate Group of Tumor Biology, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Somin
authors: S
authors: Lee
authors: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea; School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Homan
authors: H
authors: Kang
authors: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Jin-Kyoung
authors: JK
authors: Yang
authors: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Hyunmi
authors: H
authors: Lee
authors: Department of Biophysics and Chemical Biology and Department of Chemistry, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Doyk
authors: D
authors: Hwang
authors: Department of Biophysics and Chemical Biology and Department of Chemistry, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Jong Woo
authors: JW
authors: Lee
authors: Department of Chemistry Education, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Sinyoung
authors: S
authors: Jeong
authors: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Yoonjeong
authors: Y
authors: Jang
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Bong-Hyun
authors: BH
authors: Jun
authors: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea; Department of Chemistry Education, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Dae Hong
authors: DH
authors: Jeong
authors: Department of Biophysics and Chemical Biology and Department of Chemistry, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Seong Keun
authors: SK
authors: Kim
authors: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: yslee@snu.ac.kr.
authors: Yoon-Sik
authors: YS
authors: Lee
authors: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea; Program in Nano Science and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea; Graduate Group of Tumor Biology, Seoul National University, Seoul 151-742, Republic of Korea; Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea. Electronic address: mchotox@snu.ac.kr.
authors: Myung-Haing
authors: MH
authors: Cho
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004705:Endocytosis / Q000187:drug effects; D066246:ErbB Receptors / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced; D008175:Lung Neoplasms / Q000473:pathology*; D057145:Nanoshells / Q000737:chemistry / Q000648:ultrastructure; D010789:Phototherapy; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012834:Silver; D011092:Polyethylene Glycols; D006046:Gold; D004317:Doxorubicin; D066246:ErbB Receptors
keywords: Doxorubicin; Hollow-shells; Lung cancer; Photothermal therapy; Targeted drug delivery; Triggered release