title: In situ increased chemokine expression in human cervical intraepithelial neoplasia.
abstract: Chemokines play a role in tumor-inflammation and angiogenesis that could be involved in tumor progression. Monocyte chemoattractant protein-1 (MCP-1), Interleukin-8 (IL-8) and macrophage inflammatory proteins (MIP) have been identified in tumor tissues of patients with different neoplasms. Therefore, the aim of the current study was to investigate the expressions of MCP-1, IL-8 and MIP-1α, mononuclear leukocyte infiltration and leukocyte/chemokine expressions in cervical tissues from patients with cervical intraepithelial neoplasia (CIN) and controls. MCP-1, IL-8 and MIP-1α expressions and leukocyte infiltration were determined by indirect immunofluorescence in cervix biopsies from CIN patients (n=65) and 7 normal controls. Increased expressions of MCP-1 and IL-8 in CIN were observed. Increment of lymphocyte infiltration and coexpression of CD3/MCP-1 and CD3/IL-8 were found in CIN. CD3/MCP-1 cell percentage was found decreased and CD3/IL-8 percentage increased according to the CIN evolution. MIP-1α remained similar to control values. The increased expression of MCP-1 and IL-8 in cervical neoplasia may lead to tumor progression.
authors: Regional Laboratory of Virological Reference, Faculty of Medicine, Zulia University, Maracaibo, Venezuela.
authors: Yenddy
authors: Y
authors: Carrero
authors: Institute of Clinical Investigation "Dr. Américo Negrette," Faculty of Medicine, Zulia University, Maracaibo, Venezuela. Electronic address: mosquera99ve@yahoo.com.
authors: Jesús
authors: J
authors: Mosquera
authors: Regional Laboratory of Virological Reference, Faculty of Medicine, Zulia University, Maracaibo, Venezuela.
authors: Diana
authors: D
authors: Callejas
authors: Department of Immune System Diseases and Oncology, University Hospital "Príncipe de Asturias", Alcala University, Madrid, Spain.
authors: Melchor
authors: M
authors: Alvarez-Mon
mesh_terms: D018932:Chemokine CCL2 / Q000378:metabolism; D054405:Chemokine CCL3 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000378:metabolism; D002578:Uterine Cervical Dysplasia / Q000276:immunology* / Q000378:metabolism
chemical_list: C111835:CCL2 protein, human; C515429:CCL3 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D018925:Chemokines; D016209:Interleukin-8
keywords: Cervical intraepithelial neoplasia; Chemokines