title: Assessment of changes in plasma hemoglobin and potassium levels in red cell units during processing and storage.
abstract: Red cell units undergo changes during storage and processing. The study was planned to assess plasma potassium, plasma hemoglobin, percentage hemolysis during storage and to determine the effects of outdoor blood collection and processing on those parameters. Blood collection in three types of blood storage bags was done - single CPDA bag (40 outdoor and 40 in-house collection), triple CPD + SAGM bag (40 in-house collection) and quadruple CPD + SAGM bag with integral leukoreduction filter (40 in-house collection). All bags were sampled on day 0 (day of collection), day 1 (after processing), day 7, day 14 and day 28 for measurement of percentage hemolysis and potassium levels in the plasma of bag contents. There was significant increase in percentage hemolysis, plasma hemoglobin and plasma potassium level in all the groups during storage (p < 0.001). No significant difference was found between any parameter analyzed for outdoor and in-house collected single CPDA red cell units. There was significant lower percentage hemolysis (p < 0.001) and potassium (day 7 to day 14 - p < 0.05 and day 14 to day 28 - p < 0.001) in red cell units from day 7 onward until day 28 of storage in the leukoreduced quadruple bag as compared to the triple bag. The in-house single CPDA red cell units showed significantly more hemolysis (p < 0.001) as compared to the triple bags with SAGM additive solution after 28 days of storage. There is gradual increase in plasma hemoglobin and plasma potassium levels during the storage of red blood cells. Blood collection can be safely undertaken in outdoor blood donation camps even in hot summer months in monitored blood transport boxes. SAGM additive solution decreases the red cell hemolysis and allows extended storage of red cells. Prestorage leukoreduction decreases the red cell hemolysis and improves the quality of blood.
authors: Government Medical College and Hospital, Chandigarh, India. Electronic address: drnishant123@gmail.com.
authors: Nishant
authors: N
authors: Saini
authors: Government Medical College and Hospital, Chandigarh, India.
authors: Sabita
authors: S
authors: Basu
authors: Government Medical College and Hospital, Chandigarh, India.
authors: Ravneet
authors: R
authors: Kaur
authors: Government Medical College and Hospital, Chandigarh, India.
authors: Jasbinder
authors: J
authors: Kaur
mesh_terms: D000225:Adenine / Q000737:chemistry; D001771:Blood Banks; D001781:Blood Component Removal; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D005947:Glucose / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008353:Mannitol / Q000737:chemistry; D011188:Potassium / Q000097:blood*; D011446:Prospective Studies; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors
chemical_list: C037839:ADSOL; D006454:Hemoglobins; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; D011188:Potassium
keywords: Blood processing; Leukoreduction; Mannitol; Plasma hemoglobin; Storage lesions