title: An artificial CO-releasing metalloprotein built by histidine-selective metallation.
abstract: We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-[Ru(CO)2(H2O)4](2+) reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru(II)(CO)2 metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.
authors: Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal. gbernardes@medicina.ulisboa.pt.
authors: Inês S
authors: IS
authors: Albuquerque
authors: Hélia F
authors: HF
authors: Jeremias
authors: Miguel
authors: M
authors: Chaves-Ferreira
authors: Dijana
authors: D
authors: Matak-Vinkovic
authors: Omar
authors: O
authors: Boutureira
authors: Carlos C
authors: CC
authors: Romão
authors: Gonçalo J L
authors: GJ
authors: Bernardes
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002633:Chemotaxis; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D016209:Interleukin-8 / Q000737:chemistry; D008667:Metalloproteins / Q000737:chemistry*; D009504:Neutrophils / Q000166:cytology; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D016209:Interleukin-8; D008667:Metalloproteins; D006639:Histidine; D012428:Ruthenium