title: Prospective study into the value of the automated Elecsys antimüllerian hormone assay for the assessment of the ovarian growing follicle pool.
abstract: OBJECTIVE
  To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age.
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  Hospital infertility clinics and academic centers.
  
  
  METHODS
  Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles.
  
  
  METHODS
  None.
  
  
  METHODS
  AMH and antral follicle count (AFC) determined at a single visit on day 2-4 of the menstrual cycle.
  
  
  RESULTS
  There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho -0.50 for AFC and -0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively.
  
  
  CONCLUSIONS
  The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.
authors: MRC Centre for Reproductive Health, University of Edinburgh, Queen's Medical Research Institute, Edinburgh, United Kingdom. Electronic address: richard.anderson@ed.ac.uk.
authors: Richard A
authors: RA
authors: Anderson
authors: Laboratory of Hormonology and Tumour Markers, Universitair Ziekenhuis Brussel, Free University of Brussels (VUB), Brussels, Belgium.
authors: Ellen
authors: E
authors: Anckaert
authors: IVI Valencia, Valencia, Spain.
authors: Ernesto
authors: E
authors: Bosch
authors: Department of Endocrine Gynaecology and Reproductive Medicine, Hôpital Jeanne de Flandre, Centre Hospitalier de Lille, Université Lille 2, Lille, France.
authors: Didier
authors: D
authors: Dewailly
authors: MRC Centre for Reproductive Health, University of Edinburgh, Queen's Medical Research Institute, Edinburgh, United Kingdom.
authors: Cheryl E
authors: CE
authors: Dunlop
authors: UniKiD, Univ.-Frauenklinik Düsseldorf, Düsseldorf, Germany.
authors: Daniel
authors: D
authors: Fehr
authors: GyneHealth Reproductive Health Group, Manchester, United Kingdom.
authors: Luciano
authors: L
authors: Nardo
authors: Laboratory of Hormonology and Tumour Markers, Universitair Ziekenhuis Brussel, Free University of Brussels (VUB), Brussels, Belgium.
authors: Johan
authors: J
authors: Smitz
authors: Repromed, Dulwich, Australia.
authors: Kelton
authors: K
authors: Tremellen
authors: Roche Diagnostics GmbH, Penzberg, Germany.
authors: Barbara
authors: B
authors: Denk
authors: Roche Diagnostics GmbH, Penzberg, Germany.
authors: Andrea
authors: A
authors: Geistanger
authors: Roche Diagnostics International Ltd., Rotkreuz, Switzerland.
authors: Martin
authors: M
authors: Hund
mesh_terms: D000293:Adolescent; D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood*; D057205:Automation, Laboratory; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D002452:Cell Count; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000175:diagnosis* / Q000628:therapy; D006080:Ovarian Follicle / Q000166:cytology* / Q000502:physiology; D065851:Ovarian Reserve; D010062:Ovulation Induction; D011237:Predictive Value of Tests; D011379:Prognosis; D055815:Young Adult
chemical_list: D054304:Anti-Mullerian Hormone
keywords: Antimüllerian hormone; antral follicle count; ovarian follicle; ovarian reserve; reproductive life span