title: A feasibility study on quantitative analysis of glucose and fructose in lotus root powder by FT-NIR spectroscopy and chemometrics.
abstract: The feasibility of rapid analysis of glucose and fructose in lotus root powder by Fourier transform near-infrared (FT-NIR) spectroscopy was studied. Diffuse reflectance spectra were collected between 4000 and 12,432cm(-1). Calibration models established by partial least-squares regression (PLSR), interval PLS of forward (FiPLS) and backward (BiPLS), back propagation-artificial neural networks (BP-ANN) and least squares-support vector machine (LS-SVM) were compared. The optimal models for glucose and fructose were obtained by LS-SVM with the first 10 latent variables (LVs) as input. For fructose the correlation coefficients of calibration (rc) and prediction (rp), the root-mean-square errors of calibration (RMSEC) and prediction (RMSEP), and the residual predictive deviation (RPD) were 0.9827, 0.9765, 0.107%, 0.115% and 4.599, respectively. For glucose the indexes were 0.9243, 0.8286, 0.543%, 0.812% and 1.785. The results indicate that NIR spectroscopy technique with LS-SVM offers effective quantitative capability for glucose and fructose in lotus root powder.
authors: College of Quality and Technical Supervision, Hebei University, 180 Wusi East Road, Baoding, Hebei 071002, China. Electronic address: xiaoyingniu@126.com.
authors: Xiaoying
authors: X
authors: Niu
authors: College of Quality and Technical Supervision, Hebei University, 180 Wusi East Road, Baoding, Hebei 071002, China.
authors: Zhilei
authors: Z
authors: Zhao
authors: College of Quality and Technical Supervision, Hebei University, 180 Wusi East Road, Baoding, Hebei 071002, China.
authors: Kejun
authors: K
authors: Jia
authors: College of Quality and Technical Supervision, Hebei University, 180 Wusi East Road, Baoding, Hebei 071002, China.
authors: Xiaoting
authors: X
authors: Li
mesh_terms: D005240:Feasibility Studies; D005632:Fructose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D000070116:Lotus / Q000737:chemistry*; D016571:Neural Networks, Computer; D018517:Plant Roots / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D060388:Support Vector Machine
chemical_list: D011208:Powders; D005632:Fructose; D005947:Glucose
keywords: BP-ANN; FT-NIR spectroscopy; Fructose; Glucose; LS-SVM; Lotus root powder