title: Compounds isolated from the aerial part of Crataegus azarolus inhibit growth of B16F10 melanoma cells and exert a potent inhibition of the melanin synthesis.
abstract: Poor therapeutic results have been reported for treatment of malignant melanoma; therefore in this study, we have investigated inhibitory capacity of vitexin-2''-O-rhamnoside as well as the extract from which it was isolated, i.e. the ethyl acetate extract obtained from the leaves of Crataegus azarolus, on mouse melanoma (B16F10) proliferation. Cell viability was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In addition, amounts of melanin and tyrosinase were measured spectrophotometrically at 475nm. Ethyl acetate extract and vitexin-2''-O-rhamnoside exhibited significant anti-proliferative activity against B16F10 melanoma cells after incubation for 48hours with IC50s of 50μg/mL and 20μM, respectively. Furthermore, these two compounds have the ability to reduce the melanin content by inhibiting the tyrosinase activity of B16F10 cells. Thus, further investigations are merited to ascertain their potential application in treating hyperpigmentation disorders.
authors: Laboratoire de biologie cellulaire et moléculaire, faculté de médecine dentaire, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia; Unité de substances naturelles bioactives et biotechnologie UR12ES12, faculté de pharmacie de Monastir, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia.
authors: Nadia
authors: N
authors: Mustapha
authors: Laboratoire de biologie cellulaire et moléculaire, faculté de médecine dentaire, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia; Unité de substances naturelles bioactives et biotechnologie UR12ES12, faculté de pharmacie de Monastir, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia.
authors: Imèn Mokdad
authors: IM
authors: Bzéouich
authors: Unité de substances naturelles bioactives et biotechnologie UR12ES12, faculté de pharmacie de Monastir, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia.
authors: Kamel
authors: K
authors: Ghedira
authors: E.A. 4481, laboratoire de pharmacognosie, faculté de pharmacie, université de Lille 2, B.P. 83, 59006 Lille cedex, France.
authors: Thierry
authors: T
authors: Hennebelle
authors: Laboratoire de biologie cellulaire et moléculaire, faculté de médecine dentaire, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia; Unité de substances naturelles bioactives et biotechnologie UR12ES12, faculté de pharmacie de Monastir, université de Monastir, rue Avicenne, 5000 Monastir, Tunisia. Electronic address: Leila.chekir@laposte.net.
authors: Leila
authors: L
authors: Chekir-Ghedira
mesh_terms: D000818:Animals; D047310:Apigenin / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D064209:Phytochemicals / Q000302:isolation & purification* / Q000494:pharmacology; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D008543:Melanins; D064209:Phytochemicals; D010936:Plant Extracts; C021329:vitexin rhamnoside; D047310:Apigenin; D014442:Monophenol Monooxygenase
keywords: B16F10 melanoma cells; Crataegus azarolus; Melanin; Tyrosinase