title: Fabrication of an electrochemical sensor based on carbon nanotubes modified with gold nanoparticles for determination of valrubicin as a chemotherapy drug: valrubicin-DNA interaction.
abstract: In this study, an electrochemical sensor was fabricated based on gold nanoparticles/ ethylenediamine/ multi-wall carbon-nanotubes modified gold electrode (AuNPs/en/MWCNTs/AuE) for determination of valrubicin in biological samples. Valrubicin was effectively accumulated on the surface of AuNPs/en/MWCNTs/AuE and produced a pair of redox peaks at around 0.662 and 0.578V (vs. Ag/AgCl) in citrate buffer (pH4.0). The electrochemical parameters including pH, buffer, ionic strength, scan rate and size of AuNPs have been optimized. There was a good linear correlation between cathodic peak current and concentration of valrubicin in the range of 0.5 to 80.0μmolL(-1) with the detection limit of 0.018μmolL(-1) in citrate buffer (pH4.0) and 0.1molL(-1) KCl. Finally, the constructed sensor was successfully applied for determination of valrubicin in human urine and blood serum. In further studies, the different sequences of single stranded DNA probes have been immobilized on the surface of AuNPs decorated on MWCNTs to study the interaction of oligonucleotides with valrubicin.
authors: Young Researchers and Elite Club, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran; Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran. Electronic address: Hajian@iaug.ac.ir.
authors: R
authors: R
authors: Hajian
authors: Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran.
authors: Z
authors: Z
authors: Mehrayin
authors: Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran.
authors: M
authors: M
authors: Mohagheghian
authors: Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran.
authors: M
authors: M
authors: Zafari
authors: Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran.
authors: P
authors: P
authors: Hosseini
authors: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: N
authors: N
authors: Shams
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D005029:Ethylenediamines / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D010316:Particle Size; D044967:Serum / Q000737:chemistry; D014556:Urine / Q000737:chemistry
chemical_list: D005029:Ethylenediamines; D037742:Nanotubes, Carbon; C016163:valrubicin; C031234:ethylenediamine; D006046:Gold; D004317:Doxorubicin; D004247:DNA
keywords: AuNPs; DNA interaction; Electrochemical sensor; MWCNTs; Valrubicin