title: Detection and characterization of a novel human parechovirus genotype in Thailand.
abstract: Human parechoviruses (HPeV), member in the family Picornaviridae, cause respiratory symptoms primarily in infants and young children. Currently, 16 genotypes have been described based on phylogenetic analysis of VP1 sequences, all of which have a global distribution. The purpose of this study was to investigate the prevalence and genotype distribution of HPeV in Thailand. A total of 171 fecal specimens collected during October 2012 to May 2013 from children with diarrhea in Chiang Mai, Thailand were investigated for HPeV by RT-PCR and sequence analysis. HPeVs were found in 3 out of 171 (1.8%) fecal specimens tested. Of these, one was HPeV1 which is commonly detected in children with gastroenteritis and another one was uncommon HPeV14 genotype. Most interestingly, the sequence of the third HPeV positive sample (CMH-N185-12) did not cluster with any of the known 16 genotypes and therefore is proposed as a candidate HPeV genotype 17.
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Watchaporn
authors: W
authors: Chuchaona
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. Electronic address: pattara.k@cmu.ac.th.
authors: Pattara
authors: P
authors: Khamrin
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Arpaporn
authors: A
authors: Yodmeeklin
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Wilaiporn
authors: W
authors: Saikruang
authors: Pediatric Hematology Unit, Nakornping Hospital, Chiang Mai, Thailand.
authors: Tipachan
authors: T
authors: Kongsricharoern
authors: Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Nuthapong
authors: N
authors: Ukarapol
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University, School of Medicine, Tokyo, Japan.
authors: Shoko
authors: S
authors: Okitsu
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University, School of Medicine, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Hayakawa
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University, School of Medicine, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Ushijima
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Niwat
authors: N
authors: Maneekarn
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D030061:Parechovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Children; Diarrhea; Genotype; Human parechovirus; Thailand