title: Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.
abstract: RT-PCR/ESI-MS has previously demonstrated the capability to detect and identify respiratory viral pathogens in nasopharyngeal swabs. This study expands on previous research by performing a prospective evaluation of RT-PCR/ESI-MS to detect and identify Influenza A and B viruses compared to Prodesse ProFlu Plus and combined ProFlu Plus and Cepheid Xpert Flu. ProFlu Plus was also used as a gold standard for comparison for respiratory syncytial virus detection. Using ProFlu Plus as a gold standard, RT-PCR/ESI-MS had sensitivity and specificity of 82.1% (23/28) and 100% (258/258), respectively, for Influenza A, 100% (16/16) and 99.6% (269/270), respectively for Influenza B, and 88.6% (39/44) and 99.6% (241/242) for any Influenza virus. Using matching results from ProFlu Plus and Xpert Flu as a gold standard, RT-PCR/ESI-MS had 85.2% (23/27) and 100% (259/259) sensitivity and specificity respectively for Influenza A, 100% (14/14) and 99.6% (270/272), respectively for Influenza B virus. Overall, RT-PCR/ESI-MS was not as sensitive as the combined gold standard of ProFlu Plus and Xpert Flu, although it has the capability of detecting other respiratory viruses.
authors: Johns Hopkins University School of Medicine, Division of Infectious Diseases, United States. Electronic address: Jhardic1@jhmi.edu.
authors: Justin
authors: J
authors: Hardick
authors: Johns Hopkins University School of Medicine, Department of Emergency Medicine, United States.
authors: Andrea
authors: A
authors: Dugas
authors: Johns Hopkins University School of Medicine, Division of Infectious Diseases, United States.
authors: Joshua
authors: J
authors: Goheen
authors: Johns Hopkins University School of Medicine, Department of Emergency Medicine, United States.
authors: Richard
authors: R
authors: Rothman
authors: Johns Hopkins University School of Medicine, Division of Infectious Diseases, United States.
authors: Charlotte
authors: C
authors: Gaydos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D055815:Young Adult
keywords: Influenza virus; Molecular diagnostics; RT-PCR/ESI-MS; Respiratory viruses