title: A Novel Therapy for Laryngotracheal Stenosis: Treatment With Ethosomes Containing 5-Fluorouracil.
abstract: OBJECTIVE
  The purpose of our article was to explore the effect of ethosomes containing 5-fluorouracil (5-FU) with different sizes on laryngotracheal stenosis treatment.
  
  
  METHODS
  The physical characteristics of ethosomes containing 5-FU were investigated, including size, shape, and entrapment percentage. The effect of ethosomes containing 5-FU was evaluated on the airway stenosis rabbit model. The formation of fibrous/scar tissue was investigated by hematoxylin and eosin (HE) staining, and the permeation depth was observed under fluorescence microscope.
  
  
  RESULTS
  The mean sizes of 5-FU ethosomes extruded by D=50 nm and D=100 nm pore were 60±10 nm and 110±13 nm, respectively. The 5-FU entrapment percentage of ethosomes was determined to be 15% (D=60±10 nm) and 32% (D=110±13 nm). After being treated by ethosomes containing 5-FU (D=60±10 nm), the fibroblast and collagenous fiber distributed sparsely in the deep scar tissue. The permeation capability of ethosomes containing 5-FU (D=60 nm) was significantly better than ethosomes (D=110 nm). Besides, the 5-FU ethosomes resulted in less stenosis than 5-FU only.
  
  
  CONCLUSIONS
  Topical administration of 5-FU ethosomes may be a novel candidate therapy for laryngotracheal stenosis treatment.
authors: Department of Otolaryngology, Shanghai 3rd People's Hospital School of Medicine, Shanghai Jiao-tong University, Shanghai, China.
authors: Jian
authors: J
authors: Gu
authors: Department of Otolaryngology, Shanghai 3rd People's Hospital School of Medicine, Shanghai Jiao-tong University, Shanghai, China.
authors: Xiaohui
authors: X
authors: Mao
authors: Department of Otolaryngology, Shanghai 3rd People's Hospital School of Medicine, Shanghai Jiao-tong University, Shanghai, China.
authors: Cong
authors: C
authors: Li
authors: Department of Otolaryngology, Shanghai 3rd People's Hospital School of Medicine, Shanghai Jiao-tong University, Shanghai, China huafeiaoer@163.com.
authors: Huafei
authors: H
authors: Ao
authors: Department of Otolaryngology, Shanghai 3rd People's Hospital School of Medicine, Shanghai Jiao-tong University, Shanghai, China.
authors: Xizhi
authors: X
authors: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage* / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D007829:Laryngostenosis / Q000150:complications / Q000188:drug therapy*; D008081:Liposomes; D008297:Male; D011817:Rabbits; D014135:Tracheal Stenosis / Q000150:complications / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D007166:Immunosuppressive Agents; D008081:Liposomes; D005472:Fluorouracil
keywords: airway stenosis rabbit model; ethosomes containing 5-FU; ethosomes with different size; therapy for laryngotracheal stenosis