title: Postligation-desulfurization: a general approach for chemical protein synthesis.
abstract: Native chemical ligation, involving regioselective and chemoselective coupling of two unprotected peptide segments, enabled the synthesis of polypeptide with more than 200 amino acids. However, cysteine was indispensable in this synthetic technique in its initial format, which limited its further application. Thus, considerable effort has been put into breaking the restriction of cysteine-containing ligation. As a consequence, postligation-desulfurization, concerning thiol-mediated ligation followed by desulfurization, was developed. This review describes the development and recent progress on the chemical synthesis of peptides and proteins encompassing postligation-desulfurization at alanine, valine, lysine, threonine, leucine, proline, arginine, aspartic acid, glutamate, phenylalanine, glutamine, and tryptophan.
authors: Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Jimei
authors: J
authors: Ma
authors: Jing
authors: J
authors: Zeng
authors: Qian
authors: Q
authors: Wan
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D011506:Proteins