title: Prevalence and characteristics of Shiga toxin-producing Escherichia coli isolated from retail raw meats in China.
abstract: Shiga toxin-producing Escherichia coli (STEC) causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Most human infections are attributed to consumption of STEC-contaminated foodstuffs of animal origin. In this study, we evaluated the prevalence of STEC from retail raw meats collected from two geographical regions in China. The results revealed that 166 out of 853 samples were stx-positive; 63 STEC isolates were recovered from 58 stx-positive samples including pork (4.4%, 14/318), beef (11.0%, 21/191), mutton (20.6%, 26/126), chicken (0.5%, 1/205), and duck (7.7%, 1/13). Twenty-six O serogroups and 33 O:H serotypes were identified. All three stx1 subtypes and five stx2 subtypes (2a to 2e) were found in the 63 STEC isolates, among which stx2e-positive STEC isolates were the most predominant (39.7%), followed by stx1c only (20.6%), stx1c+stx2b (14.3%), and stx1a only (9.5%). STEC isolates carried virulence genes eae (6.3%), ehxA (36.5%), katP (4.8%), astA (11.1%), and subA (36.5%). Of the four adherence-associated genes tested, toxB was absent, whereas saa, paa, and efa1 were present in 28, three, and one STEC isolates respectively. The STEC isolates were divided into 50 PFGE patterns and 33 sequence types. STEC from different sources and geographical regions were separated by PFGE and MLST. Our results revealed that there is a high genetic diversity of STEC in retail raw meats, some of which have potential to cause human diseases.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Xiangning
authors: X
authors: Bai
authors: Zigong Center for Disease Control and Prevention, Zigong, Sichuan Province, China.
authors: Hong
authors: H
authors: Wang
authors: Qinghai Institute for Endemic Disease Prevention and Control, Xining, Qinghai Province, China.
authors: Youquan
authors: Y
authors: Xin
authors: Qinghai Institute for Endemic Disease Prevention and Control, Xining, Qinghai Province, China.
authors: Rongjie
authors: R
authors: Wei
authors: Qinghai Institute for Endemic Disease Prevention and Control, Xining, Qinghai Province, China.
authors: Xinyuan
authors: X
authors: Tang
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Ailan
authors: A
authors: Zhao
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Hui
authors: H
authors: Sun
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Wang
authors: W
authors: Zhang
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Yan
authors: Y
authors: Wang
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
authors: Yanmei
authors: Y
authors: Xu
authors: Zigong Center for Disease Control and Prevention, Zigong, Sichuan Province, China.
authors: Zhengdong
authors: Z
authors: Zhang
authors: Zigong Center for Disease Control and Prevention, Zigong, Sichuan Province, China.
authors: Qun
authors: Q
authors: Li
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China. Electronic address: xujianguo@icdc.cn.
authors: Jianguo
authors: J
authors: Xu
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China. Electronic address: xiongyanwen@icdc.cn.
authors: Yanwen
authors: Y
authors: Xiong
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002681:China; D004372:Ducks; D014644:Genetic Variation; D008460:Meat / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D015995:Prevalence; D012703:Serotyping; D012756:Sheep; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D013552:Swine; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Multi-locus sequence typing; Pulsed-field gel electrophoresis; Raw meat; Shiga toxin-producing Escherichia coli (STEC); Virulence gene