title: Quercetin inhibits vascular endothelial growth factor-induced choroidal and retinal angiogenesis in vitro.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of quercetin on vascular endothelial growth factor (VEGF)-induced choroidal and retinal angiogenesis in vitro using a rhesus macaque choroid-retinal endothelial (RF/6A) cell line.
  
  
  METHODS
  RF/6A cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. Then the cells were treated with different concentrations (from 0 to 100 μM) of quercetin and 100 ng/ml VEGF. The cell proliferation was assessed using cholecystokinin octapeptide dye. The cell migration was investigated by a Transwell assay. The tube formation was measured on Matrigel. Furthermore, the impact of quercetin's effects on VEGF-induced activation of VEGF receptor 2 (VEGFR-2) downstream signal pathways was tested by Western blot analysis.
  
  
  RESULTS
  Quercetin inhibits RF/6A cell proliferation in a dose-dependent fashion: 22.7, 31.5 and 36.7% inhibition on treatment with 10, 50 and 100 μM quercetin, respectively. VEGF-induced migration and tube formation of RF/6A cells were also significantly inhibited by quercetin in a dose-dependent manner. Quercetin inhibits VEGF-induced VEGFR-2 downstream signal pathways of RF/6A.
  
  
  CONCLUSIONS
  The results show that quercetin inhibits VEGF-induced cell proliferation, migration and tube formation of RF/6A. We suggest that quercetin inhibits VEGF-induced choroidal and retinal angiogenesis in vitro. Collectively, the findings in the present study suggest that quercetin inhibits VEGF-induced choroidal and retinal angiogenesis by targeting the VEGFR-2 pathway. This suggests that quercetin is a choroidal and retinal angiogenesis inhibitor.
authors: Department of Ophthalmology, Peking University People's Hospital, Beijing, China.
authors: Fangting
authors: F
authors: Li
authors: Yujing
authors: Y
authors: Bai
authors: Min
authors: M
authors: Zhao
authors: Lvzhen
authors: L
authors: Huang
authors: Shanshan
authors: S
authors: Li
authors: Xiaoxin
authors: X
authors: Li
authors: Yi
authors: Y
authors: Chen
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002829:Choroid / Q000166:cytology; D020256:Choroidal Neovascularization / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D008253:Macaca mulatta; D011794:Quercetin / Q000494:pharmacology*; D012160:Retina / Q000166:cytology; D015861:Retinal Neovascularization / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*
chemical_list: D020533:Angiogenesis Inhibitors; D000975:Antioxidants; D042461:Vascular Endothelial Growth Factor A; D011794:Quercetin