title: Oxidative, metabolic, and apoptotic responses of Schwann cells to high glucose levels.
abstract: The specific response of murine Schwann cells IMS32 to acute and chronic hyperglycemia conditions was evaluated. The pathophysiological alterations were studied to deepening the role of Schwann cells in diabetes-related neurotoxicity and to assess a model to screen new protective molecules. IMS32 were incubated with 30 and 56 mM glucose for 48 h and 7 and 14 days, and markers of oxidative stress, apoptosis, and polyol pathway were evaluated. High glucose induced O(2) -production and lipid peroxidation at all time point whereas Caspase 3 activity was induced only after 14 days. Aldose reductase activity and expression were significantly increased after 48 h and 14 days, respectively. Our results describe the response of Schwann cells to high glucose conditions and suggest the use of IMS32 for the screening of protective molecules in diabetes-induced neuropathy.
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy. lorenzo.cinci@unifi.it.
authors: Lorenzo
authors: L
authors: Cinci
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy.
authors: Francesca
authors: F
authors: Corti
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy.
authors: Lorenzo
authors: L
authors: Di Cesare Mannelli
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy.
authors: Laura
authors: L
authors: Micheli
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy.
authors: Matteo
authors: M
authors: Zanardelli
authors: Department of Neuroscience, Psychology, Drug Research and Child health (NEUROFARBA), University of Florence, viale Pieraccini 6, 50139, Florence, Italy.
authors: Carla
authors: C
authors: Ghelardini
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D000449:Aldehyde Reductase; D053148:Caspase 3; D005947:Glucose
keywords: Diabetes; Diabetic Peripheral Neuropathy; Hyperglycemia; Schwann Cells