title: Acute toxicity of cypermethrin on the freshwater mussel Unio gibbus.
abstract: Cypermethrin is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control, food stuff protection and disease vector control. We investigate the potential of cypermethrin to induce oxidative stress and enzyme activities within the gills of freshwater mussel Unio gibbus. This study was carried out under laboratory conditions using two nominal cypermethrin concentrations C1 (100µg/L) and C2 (150µg/L) during 96h. The measured concentrations of cypermethrin using GC-MS-MS in the treatment aquariums were respectively 59.7 µg/L and 97.5µg/L. Antioxidant enzyme activities (superoxide dismutase (SOD) and catalase (CAT)) as well as H2O2, malondialdehyde (MDA) and protein carbonyl (PCO) levels were assessed. An exposure during 96h induced the SOD activity at the highest concentration. The CAT activity and H2O2 level were increased significantly (P<0.05) in gills following a dose-dependent profile. Cypermethrin also generated an increase in malondialdehyde (MDA) levels reaching the highest value at the high concentration. The considered parameters can be used as biomarkers of exposure to cypermethrin. Freshwater mussel U. gibbus can be potentially employed in biomonitoring surveys of such threatened ecosystems.
authors: Laboratoire de Biosurveillance de l'Environnement (LBE), Unité d'Ecotoxicologie et d'Ecologie Côtière (GREEC), Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
authors: Abdelhafidh
authors: A
authors: Khazri
authors: Laboratoire de Biosurveillance de l'Environnement (LBE), Unité d'Ecotoxicologie et d'Ecologie Côtière (GREEC), Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
authors: Badreddine
authors: B
authors: Sellami
authors: Laboratoire de Biosurveillance de l'Environnement (LBE), Unité d'Ecotoxicologie et d'Ecologie Côtière (GREEC), Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
authors: Mohamed
authors: M
authors: Dellali
authors: Water and Soil Quality Research Group, Department of Environmental Chemistry, IDÆA-CSIC, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Cayo
authors: C
authors: Corcellas
authors: Water and Soil Quality Research Group, Department of Environmental Chemistry, IDÆA-CSIC, Jordi Girona, 18, 08034 Barcelona, Spain. Electronic address: eeeqam@cid.csic.es.
authors: Ethel
authors: E
authors: Eljarrat
authors: Water and Soil Quality Research Group, Department of Environmental Chemistry, IDÆA-CSIC, Jordi Girona, 18, 08034 Barcelona, Spain.
authors: Damià
authors: D
authors: Barceló
authors: Laboratoire de Biosurveillance de l'Environnement (LBE), Unité d'Ecotoxicologie et d'Ecologie Côtière (GREEC), Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
authors: Ezzeddine
authors: E
authors: Mahmoudi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007306:Insecticides / Q000032:analysis / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011722:Pyrethrins / Q000032:analysis / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D049881:Unio / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007306:Insecticides; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017160:cypermethrin; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Biomarkers; Cypermethrin; GC–MS–MS; Unio gibbus