title: Comparative lectinology: Delineating glycan-specificity profiles of the chicken galectins using neoglycoconjugates in a cell assay.
abstract: A major aspect of carbohydrate-dependent galectin functionality is their cross-linking capacity. Using a cell surface as biorelevant platform for galectin binding and a panel of 40 glycans as sensor part of a fluorescent polyacrylamide neoglycopolymer for profiling galectin reactivity, properties of related proteins can be comparatively analyzed. The group of the chicken galectins (CGs) is an especially suited system toward this end due to its relatively small size, compared with mammalian galectins. The experiments reveal particularly strong reactivity toward N-acetyllactosamine repeats for all tested CGs and shared reactivity of CG-1A and CG-2 to histo-blood group ABH determinants. In cross-species comparison, CG-1B's properties closely resembled those of human galectin-1, as was the case for the galectin-2 (but not galectin-3) ortholog pair. Although binding-site architectures are rather similar, reactivity patterns can well differ.
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Eugenia M
authors: EM
authors: Rapoport
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Varvara K
authors: VK
authors: Matveeva
authors: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, Munich 80539, Germany.
authors: Herbert
authors: H
authors: Kaltner
authors: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, Munich 80539, Germany.
authors: Sabine
authors: S
authors: André
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Olga A
authors: OA
authors: Vokhmyanina
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Galina V
authors: GV
authors: Pazynina
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Vyacheslav V
authors: VV
authors: Severov
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Ivan M
authors: IM
authors: Ryzhov
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Elena Yu
authors: EY
authors: Korchagina
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia.
authors: Ivan M
authors: IM
authors: Belyanchikov
authors: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, Munich 80539, Germany.
authors: Hans-J
authors: HJ
authors: Gabius
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, ul. Miklukho-Maklaya16/10, Moscow, Russia professorbovin@yandex.ru.
authors: Nicolai V
authors: NV
authors: Bovin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D002645:Chickens; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D011134:Polysaccharides
keywords: carbohydrates; glycosylation; lectin; phylogenesis; sialylation