title: Characterization of the complete genome of Barley yellow striate mosaic virus reveals a nested gene encoding a small hydrophobic protein.
abstract: Barley yellow striate mosaic virus (BYSMV), a member of the genus Cytorhabdovirus, causes serious crop losses in agriculture. Here, we have cloned the BYSMV-derived small interfering RNAs (siRNAs), assembled the siRNAs and used RT-PCR to reconstruct the BYSMV genome. The genome consists of 12,706 nucleotides and encodes ten predicted genes from the antigenomic strand. The major BYSMV structural proteins share identities ranging from 35% to 62% with northern cereal mosaic virus (NCMV) counterparts. A notable difference is that BYSMV contains three transcriptional units residing between the P and M genes compared with four units in the corresponding region of NCMV. Unexpectedly, the middle mRNA in this region encodes gene5 nested in an alternative frame within gene4 via a leaky scanning mechanism. The gene5 encodes a small hydrophobic protein targeting to the endoplasmic reticulum (ER). To our knowledge, this is the first report of nested gene in plant rhabdoviruses.
authors: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: yantengforever@gmail.com.
authors: Teng
authors: T
authors: Yan
authors: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: jingrongzhu5@gmail.com.
authors: Jing-Rong
authors: JR
authors: Zhu
authors: Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, Hebei Province, PR China. Electronic address: chmrdv@163.com.
authors: Dianping
authors: D
authors: Di
authors: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: gaoqianngnuli@gmail.com.
authors: Qiang
authors: Q
authors: Gao
authors: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: zhangyongliang7@gmail.com.
authors: Yongliang
authors: Y
authors: Zhang
authors: Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, Hebei Province, PR China. Electronic address: zhangaihong08@163.com.
authors: Aihong
authors: A
authors: Zhang
authors: Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, Hebei Province, PR China. Electronic address: yanchong986@126.com.
authors: Chong
authors: C
authors: Yan
authors: Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, Hebei Province, PR China. Electronic address: miao5058345@163.com.
authors: Hongqin
authors: H
authors: Miao
authors: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: wangxianbing@cau.edu.cn.
authors: Xian-Bing
authors: XB
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005814:Genes, Viral; D016679:Genome, Viral; D001467:Hordeum / Q000821:virology; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D032041:Nested Genes; D012367:RNA, Viral / Q000235:genetics*; D012209:Rhabdoviridae / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Ancillary gene; Barley yellow striate mosaic virus; Endoplasmic reticulum; Nested gene; Plant rhabdovirus