title: Pyrosequencing reveals fungal communities in the rhizosphere of Xinjiang Jujube.
abstract: Fungi are important soil components as both decomposers and plant symbionts and play a major role in ecological and biogeochemical processes. However, little is known about the richness and structure of fungal communities. DNA sequencing technologies allow for the direct estimation of microbial community diversity, avoiding culture-based biases. We therefore used 454 pyrosequencing to investigate the fungal communities in the rhizosphere of Xinjiang jujube. We obtained no less than 40,488 internal transcribed spacer (ITS) rDNA reads, the number of each sample was 6943, 6647, 6584, 6550, 6860, and 6904, and we used bioinformatics and multivariate statistics to analyze the results. The index of diversity showed greater richness in the rhizosphere fungal community of a 3-year-old jujube than in that of an 8-year-old jujube. Most operational taxonomic units belonged to Ascomycota, and taxonomic analyses identified Hypocreales as the dominant fungal order. Our results demonstrated that the fungal orders are present in different proportions in different sampling areas. Redundancy analysis (RDA) revealed a significant correlation between soil properties and the abundance of fungal phyla. Our results indicated lower fungal diversity in the rhizosphere of Xinjiang jujube than that reported in other studies, and we hope our findings provide a reference for future research.
authors: College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China.
authors: Peng
authors: 0000-0003-4997-0713
authors: P
authors: Liu
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors: Xiao-Hui
authors: XH
authors: Wang
authors: Research Institute of Forest in Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.
authors: Jian-Gui
authors: JG
authors: Li
authors: Research Institute of Forest in Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.
authors: Wei
authors: W
authors: Qin
authors: College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China.
authors: Cheng-Ze
authors: CZ
authors: Xiao
authors: College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China.
authors: Xu
authors: X
authors: Zhao
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors: Hong-Xia
authors: HX
authors: Jiang
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors: Jun-Kang
authors: JK
authors: Sui
authors: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors: Rong-Bo
authors: RB
authors: Sa
authors: College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China.
authors: Wei-Yan
authors: WY
authors: Wang
authors: College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China.
authors: Xun-Li
authors: XL
authors: Liu
mesh_terms: D044822:Biodiversity; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D038821:Mycorrhizae / Q000235:genetics*; D027343:Rhizome / Q000382:microbiology*; D012988:Soil Microbiology; D031957:Ziziphus / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal