title: Preparation of electrochemical biosensor for detection of organophosphorus pesticides.
abstract: Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by using glutaraldehyde as a coupling agent. The carbon nanotubes paste working electrode was constructed for amperometric measurement at a potential of +0.8 V. The biosensor showed optimum response at pH 8.0 with incubation temperature of 40°C. K m and I max for substrate (methyl parathion) were 322.58 µM and 1.1 µA, respectively. Evaluation study showed a correlation of 0.985, which was in agreement with the standard method. The OPH biosensor lost 50% of its initial activity after its regular use for 25 times over a period of 50 days when stored in 0.1 M sodium phosphate buffer, pH 8.0 at 4°C. No interference was observed by interfering species.
authors: Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India.
authors: Ashish
authors: A
authors: Gothwal
authors: Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India.
authors: Puneet
authors: P
authors: Beniwal
authors: Department of Bio- & Nanotechnology, Guru Jambeshwar University of Science & Technology, Hisar 125001, India.
authors: Vikas
authors: V
authors: Dhull
authors: Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India.
authors: Vikas
authors: 0000-0002-6073-7405
authors: V
authors: Hooda