title: Multifunctional polyurethane sponge for polymerase chain reaction enhancement.
abstract: Selective filtering of target biomaterials from impurities is an important task in DNA amplification through polymerase chain reaction (PCR) enhancement and gene identification to save endangered animals and marine species. Conventional gene extraction methods require complicated steps, skilled persons, and expensive chemicals and instruments to improve DNA amplification. Herein, we proposed an alternative method for overcoming such challenges by imparting secondary functionality using commercially available polyurethane (PU) sponges and cost-effective fabrication approaches through polydopamine and polysiloxane coatings. The porous, highly flexible, and chemically modified superhydrophilic and superhydrophobic PU sponges allow large surface areas and mechanically stable frames for effective extraction of genomic DNA through selective filtering of fish tissues and oils. Furthermore, these chemically modified PU sponges allow separation of genes and improvement of PCR for DNA amplification for the identification of fish species. The combination of a simple fabrication method and functionalized PU sponges could be a useful platform for PCR enhancement and gene-based identification of species for practical applications.
authors: Department of Chemical & Biomolecular Engineering, KAIST , 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Seunghwan
authors: S
authors: Seok
authors: Sujeong
authors: S
authors: Shin
authors: Tae Jae
authors: TJ
authors: Lee
authors: Jae-Min
authors: JM
authors: Jeong
authors: MinHo
authors: M
authors: Yang
authors: Do Hyun
authors: DH
authors: Kim
authors: Jung Youn
authors: JY
authors: Park
authors: Seok Jae
authors: SJ
authors: Lee
authors: Bong Gill
authors: BG
authors: Choi
authors: Kyoung G
authors: KG
authors: Lee
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry; D010473:Perciformes / Q000235:genetics; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D007211:Indoles; D011108:Polymers; D011140:Polyurethanes; D012833:Siloxanes; C568283:polydopamine; D004247:DNA
keywords: DNA; PCR; filter; polyurethane; superhydrophilic; superhydrophobic