title: Characterization of a new endo-type alginate lyase from Vibrio sp. W13.
abstract: A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 °C. Interestingly, Algb possessed broader substrate specificity. It showed activities toward both polyM (poly β-D-mannuronate) and polyG (poly α-L-guluronate). Furthermore, K(m) values of Algb toward alginate (0.67 mg/ml) and polyMG (0.50 mg/ml) are lower than those toward polyG (1.04 mg/ml) and polyM (6.90 mg/ml). The TLC and ESI-MS analysis suggested that Algb mainly released oligosaccharides with DP of 2-5 from the four kinds of substrates in an endolytic manner. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DP.
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Benwei
authors: B
authors: Zhu
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors: Haidong
authors: H
authors: Tan
authors: National Glycoengineering Research Center, Shandong University, Jinan 250100, PR China.
authors: Yuqi
authors: Y
authors: Qin
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors: Qingsong
authors: Q
authors: Xu
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: duyg@dicp.ac.cn.
authors: Yuguang
authors: Y
authors: Du
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China. Electronic address: yinheng@dicp.ac.cn.
authors: Heng
authors: H
authors: Yin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D007477:Ions; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D012965:Sodium Chloride / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity / Q000187:drug effects; D014733:Vibrio / Q000201:enzymology*
chemical_list: D007477:Ions; D008670:Metals; D011994:Recombinant Proteins; D012965:Sodium Chloride; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase
keywords: Alginate lyase; Polysaccharide lyase family 7; Vibrio sp.