title: Biological interactions between mercury and selenium in distribution and detoxification processes in mice under controlled exposure. Effects on selenoprotein.
abstract: Antagonistic interactions between mercury (Hg) and selenium (Se), were evaluated in mouse (Mus musculus), as a mammalian model, in a series of controlled exposure experiments. The beneficial effect of Se against Hg toxicity involves a variety of biochemical and toxicological processes that have not been clarified yet. For this purpose, a metallomic workflow based on the use of size-exclusion chromatography (SEC) with inductively coupled plasma mass spectrometry (ICP-MS) detection was complemented with the speciation of selenoproteins and low molecular mass selenium species in serum and liver cytosolic extracts using a multidimensional approach based on SEC-AF-HPLC-ICPMS, using species-unspecific isotope dilution (SUID)-ICP-MS for selenium quantification. The results showed potential interactions between Hg/Se in organs and serum related to accumulation and detoxification processes, in addition to the effects of mercury on selenoproteins in hepatic cytosolic extracts and bloodstream when both elements are administrated at the same time. These results provide information about elements distribution, interactions and homeostasis and reveal the potential of metallomic approaches in exposure experiments.
authors: Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Research Center on Health and Environment (CYSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain. Electronic address: mangel.garcia@dqcm.uhu.es.
authors: M A
authors: MA
authors: García-Sevillano
authors: Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Research Center on Health and Environment (CYSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain. Electronic address: gema.moro@dqcm.uhu.es.
authors: G
authors: G
authors: Rodríguez-Moro
authors: Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Research Center on Health and Environment (CYSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain. Electronic address: tamara@dqcm.uhu.es.
authors: T
authors: T
authors: García-Barrera
authors: International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain; Department of Environmental Biology and Public Health, Cell Biology, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain. Electronic address: navarro@uhu.es.
authors: F
authors: F
authors: Navarro
authors: Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Research Center on Health and Environment (CYSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain. Electronic address: ariza@uhu.es.
authors: J L
authors: JL
authors: Gómez-Ariza
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008628:Mercury / Q000032:analysis / Q000097:blood / Q000378:metabolism* / Q000633:toxicity*; D051379:Mice; D008958:Models, Molecular; D012643:Selenium / Q000032:analysis / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology*; D051140:Selenoproteins / Q000378:metabolism*
chemical_list: D051140:Selenoproteins; D008628:Mercury; D012643:Selenium
keywords: Mercury; Metal interactions; Metallomics; Mus musculus; Selenium; Selenoproteins