title: Human papillomavirus and breast cancer: no evidence of association in a Spanish set of cases.
abstract: OBJECTIVE
  Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases.
  
  
  METHODS
  Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay.
  
  
  RESULTS
  A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease.
  
  
  CONCLUSIONS
  Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.
authors: Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain Breast Cancer Unit, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain mvernet@hospitaldelmar.cat.
authors: Maria
authors: M
authors: Vernet-Tomas
authors: Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain.
authors: Marisa
authors: M
authors: Mena
authors: Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain CIBER Epidemiology and Public Health, Barcelona, Spain.
authors: Laia
authors: L
authors: Alemany
authors: Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain.
authors: Ignacio
authors: I
authors: Bravo
authors: Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain CIBER Epidemiology and Public Health, Barcelona, Spain.
authors: Silvia
authors: S
authors: De Sanjosé
authors: Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain.
authors: Pau
authors: P
authors: Nicolau
authors: Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain.
authors: Alba
authors: A
authors: Bergueiro
authors: Breast Cancer Unit, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain Pathology Department, Hospital del Mar, Barcelona, Spain.
authors: Josep Maria
authors: JM
authors: Corominas
authors: Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain Pathology Department, Hospital del Mar, Barcelona, Spain.
authors: Sergi
authors: S
authors: Serrano
authors: Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain.
authors: Ramon
authors: R
authors: Carreras
authors: Pathology Department, Hospital del Mar, Barcelona, Spain.
authors: Belen
authors: B
authors: Lloveras
mesh_terms: D000368:Aged; D001483:Base Sequence; D001943:Breast Neoplasms / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008875:Middle Aged; D009426:Netherlands; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D013030:Spain
chemical_list: D017931:DNA Primers; D004279:DNA, Viral
keywords: Breast cancer; human papillomavirus; polymerase chain reaction