title: Pulmonary CD103 expression regulates airway inflammation in asthma.
abstract: Although CD103(+) cells recently emerged as key regulatory cells in the gut, the role of CD103 ubiquitous expression in the lung and development of allergic airway disease has never been studied. To answer this important question, we evaluated the response of Cd103(-/-) mice in two separate well-described mouse models of asthma (ovalbumin and house dust mite extract). Pulmonary inflammation was assessed by analysis of bronchoalveolar lavage content, histology, and cytokine response. CD103 expression was analyzed on lung dendritic cells and T cell subsets by flow cytometry. Cd103(-/-) mice exposed to antigens developed exacerbated lung inflammation, characterized by increased eosinophilic infiltration, severe tissue inflammation, and altered cytokine response. In wild-type mice exposed to house dust mite, CD103(+) dendritic cells are increased in the lung and an important subset of CD4(+) T cells, CD8(+) T cells, and T regulatory cells express CD103. Importantly, Cd103(-/-) mice presented a deficiency in the resolution phase of inflammation, which supports an important role for this molecule in the control of inflammation severity. These results suggest an important role for CD103 in the control of airway inflammation in asthma.
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: Emilie
authors: E
authors: Bernatchez
authors: The Biomedical Research Center, University of British Columbia, Vancouver, BC, Canada.
authors: Matthew J
authors: MJ
authors: Gold
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: Anick
authors: A
authors: Langlois
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: Anne-Marie
authors: AM
authors: Lemay
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: Julyanne
authors: J
authors: Brassard
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: Nicolas
authors: N
authors: Flamand
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and.
authors: David
authors: D
authors: Marsolais
authors: The Biomedical Research Center, University of British Columbia, Vancouver, BC, Canada.
authors: Kelly M
authors: KM
authors: McNagny
authors: Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada; and marie-renee.blanchet@criucpq.ulaval.ca.
authors: Marie-Renee
authors: MR
authors: Blanchet
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001249:Asthma / Q000276:immunology / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology; D015870:Gene Expression; D007249:Inflammation / Q000378:metabolism; D039001:Integrin alpha Chains / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D039001:Integrin alpha Chains; C091367:alpha E integrins
keywords: CD103; T regulatory cells; asthma; dendritic cells