title: Functional classification and biochemical characterization of a novel rho class glutathione S-transferase in Synechocystis PCC 6803.
abstract: We report a novel class of glutathione S-transferase (GST) from the model cyanobacterium Synechocystis PCC 6803 (sll1545) which catalyzes the detoxification of the water pollutant dichloroacetate and also shows strong glutathione-dependent peroxidase activity representing the classical activities of zeta and theta/alpha class respectively. Interestingly, sll1545 has very low sequence and structural similarity with these classes. This is the first report of dichloroacetate degradation activity by any bacterial GST. Based on these results we classify sll1545 to a novel GST class, rho. The present data also indicate potential biotechnological and industrial applications of cyanobacterial GST in dichloroacetate-polluted areas.
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Tripti
authors: T
authors: Pandey
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Gaurav
authors: G
authors: Chhetri
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Ramesh
authors: R
authors: Chinta
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Bijay
authors: B
authors: Kumar
authors: Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 208024, India.
authors: Dev Bukhsh
authors: DB
authors: Singh
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Timir
authors: T
authors: Tripathi
authors: Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
authors: Arvind Kumar
authors: AK
authors: Singh
keywords: Bioremediation; CDNB, 1-chloro-2,4-dinitrobenzene; Cyanobacteria; DCA, dichloroacetate; Detoxification; Dichloroacetate; GSH, reduced glutathione; GST, glutathione S-transferase; GSTZ, glutathione S-transferase zeta; Glutathione; Water pollutant