title: Bursopentin (BP5) protects dendritic cells from lipopolysaccharide-induced oxidative stress for immunosuppression.
abstract: Dendritic cells (DCs) play a vital role in the regulation of immune-mediated inflammatory diseases. Thus, DCs have been regarded as a major target for the development of immunomodulators. However, oxidative stress could disturb inflammatory regulation in DCs. Here, we examined the effect of bursopentine (BP5), a novel pentapeptide isolated from chicken bursa of fabricius, on the protection of DCs against oxidative stress for immunosuppression. BP5 showed potent protective effects against the lipopolysaccharide (LPS)-induced oxidative stress in DCs, including nitric oxide, reactive oxygen species and lipid peroxidation. Furthermore, BP5 elevated the level of cellular reductive status through increasing the reduced glutathione (GSH) and the GSH/GSSG ratio. Concomitant with these, the activities of several antioxidative redox enzymes, including glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD), were obviously enhanced. BP5 also suppressed submucosal DC maturation in the LPS-stimulated intestinal epithelial cells (ECs)/DCs coculture system. Finally, we found that heme oxygenase 1 (HO-1) was remarkably upregulated by BP5 in the LPS-induced DCs, and played an important role in the suppression of oxidative stress and DC maturation. These results suggested that BP5 could protect the LPS-activated DCs against oxidative stress and have potential applications in DC-related inflammatory responses.
authors: Key Lab of Animal Physiology and Biochemistry of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
authors: Tao
authors: T
authors: Qin
authors: Key Lab of Animal Physiology and Biochemistry of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
authors: Yinyan
authors: Y
authors: Yin
authors: Key Lab of Animal Physiology and Biochemistry of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
authors: Qinghua
authors: Q
authors: Yu
authors: Key Lab of Animal Physiology and Biochemistry of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.
authors: Qian
authors: Q
authors: Yang
mesh_terms: D000818:Animals; D002374:Catalase / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D005978:Glutathione / Q000276:immunology / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000276:immunology / Q000378:metabolism; D007108:Immune Tolerance / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D009842:Oligopeptides; D017382:Reactive Oxygen Species; C569205:cysteinyl-lysyl-aspartyl-valyl-tyrosine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione