title: A separation-integrated cascade reaction to overcome thermodynamic limitations in rare-sugar synthesis.
abstract: Enzyme cascades combining epimerization and isomerization steps offer an attractive route for the generic production of rare sugars starting from accessible bulk sugars but suffer from the unfavorable position of the thermodynamic equilibrium, thus reducing the yield and requiring complex work-up procedures to separate pure product from the reaction mixture. Presented herein is the integration of a multienzyme cascade reaction with continuous chromatography, realized as simulated moving bed chromatography, to overcome the intrinsic yield limitation. Efficient production of D-psicose from sucrose in a three-step cascade reaction using invertase, D-xylose isomerase, and D-tagatose epimerase, via the intermediates D-glucose and D-fructose, is described. This set-up allowed the production of pure psicose (99.9%) with very high yields (89%) and high enzyme efficiency (300 g of D-psicose per g of enzyme).
authors: Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel (Switzerland).
authors: Nina
authors: N
authors: Wagner
authors: Andreas
authors: A
authors: Bosshart
authors: Jurek
authors: J
authors: Failmezger
authors: Matthias
authors: M
authors: Bechtold
authors: Sven
authors: S
authors: Panke
mesh_terms: D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D019998:Racemases and Epimerases / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D002241:Carbohydrates; D019998:Racemases and Epimerases
keywords: biocatalysis; carbohydrates; enzymes; isomerases; synthetic methods