title: The phasin PhaF controls bacterial shape and size in a network-forming strain of Pseudomonas putida.
abstract: Pseudomonas putida N, a poly-3-hydroxyalkonate (PHA)-producing bacterium, showing ampicillin resistance, is an unusual strain. In the presence of this antibiotic, it grows as giant cells (25-50μm) forming complex networks inter-connected by micro-tubular structures. The transformation of this bacterium with a plasmid containing the gene phaF, which encodes a phasin involved in the molecular architecture of the PHA-granules, (i) restores the wild-type phenotype by reducing both bacterial size and length (coco-bacilli ranging between 0.5 and 3μm), and (ii) increases ampicillin resistance by more than 100-fold.
authors: Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, 24007 León, Spain.
authors: José I
authors: JI
authors: Obeso
authors: Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, 24007 León, Spain.
authors: José L
authors: JL
authors: Gómez-Botrán
authors: Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, 24007 León, Spain.
authors: Elías R
authors: ER
authors: Olivera
authors: Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, 24007 León, Spain. Electronic address: jm.luengo@unileon.es.
authors: José M
authors: JM
authors: Luengo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D037121:Plant Lectins / Q000235:genetics / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism; D016958:Pseudomonas putida / Q000166:cytology* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D037121:Plant Lectins; D011091:Polyesters; C008825:phasin
keywords: Ampicillin; P. putida; PHAs; Phasins; Poly-3-hydroxyalkanoates