title: Manganese superoxide dismutase (MnSOD Val-9Ala) gene polymorphism and susceptibility to gastric cancer.
abstract: BACKGROUND
  Oxidative stress caused by the generation of reactive oxygen species plays an important role in human carcinogenesis. Manganese superoxide dismutase (MnSOD) Val-9Ala in the mitochondrial target sequence is the best known polymorphism of this enzyme. The purpose of the current research was to assess the association of MnSOD Val-9Ala genotypes with the risk of gastric cancer.
  
  
  METHODS
  This case- control study covered 54 gastric cancer patients compared to 100 cancer free subjects as controls. Extraction of DNA was performed on bioptic samples and genotypes were identified with a polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  The frequencies of MnSOD Ala/Ala, Ala/Val and Val/Val genotypes in healthy individuals were 24.3, 66.7 and 9%, respectively. However, in gastric cancer patients, Ala/Ala, Ala/Val and Val/Val were observed in 24.0, 48.0 and 28.0% (p=0.01). In patients the frequency of MnSOD Val allele was higher (52%) compared to that in controls (42%).
  
  
  CONCLUSIONS
  The results of this study show a positive association between MnSOD Val-9Ala gene polymorphism and risk of gastric cancer disease in Iranian population.
authors: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran E-mail : kyari@kums.ac.ir.
authors: Mohammad-Taher
authors: MT
authors: Moradi
authors: Kheirollah
authors: K
authors: Yari
authors: Zohreh
authors: Z
authors: Rahimi
authors: Elham
authors: E
authors: Kazemi
authors: Mehrdad
authors: M
authors: Shahbazi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D013482:Superoxide Dismutase