title: Downregulation of TPTE2P1 Inhibits Migration and Invasion of Gallbladder Cancer Cells.
abstract: Human gallbladder cancer is a rare malignancy disease but having poor prognosis over the world. Previous studies have put forward that PTEN is a tumour suppressor in regulating many cellular processes, similar activities have been observed for its mammal homologue TPTE2. In this study, we attempted to unravel the underlying mechanistic basis of the role of TPTE2 and its pseudogene TPTE2P1 in gallbladder cancer. We employed lentivirus-mediated RNA interference as an efficient tool to silence endogenous TPTE2P1 transcription in the gallbladder cancer cell line GBC-SD/M. The effects of TPTE2P1 on cell migration and invasion were determined by transwell assays. We figured that depletion of TPTE2P1 remarkably inhibited gallbladder cancer cell migration and invasion capacity in vitro and elevated the expression of β-catenin via epithelial-mesenchymal transition signalling. Our findings revealed the functional role of TPTE2P1 in human gallbladder cancer and suggested that TPTE2P1 could serve as a promising therapeutic target and a palliation option in human gallbladder cancer.
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Wenjie
authors: W
authors: Lv
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Lei
authors: L
authors: Wang
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Jianhua
authors: J
authors: Lu
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Jiasheng
authors: J
authors: Mu
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Yingbin
authors: Y
authors: Liu
authors: Department of General Surgery, Xinhua Hospital, Affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China.
authors: Ping
authors: P
authors: Dong
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D054637:Dual-Specificity Phosphatases / Q000235:genetics* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D058990:Molecular Targeted Therapy / Q000379:methods; D011544:Pseudogenes; D034622:RNA Interference; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D051176:beta Catenin; D054637:Dual-Specificity Phosphatases; C576347:TPTE2 protein, human
keywords: RNA interference; TPTE2P1; cell migration; gallbladder cancer; β-catenin