title: Non-excitatory electrical stimulation attenuates myocardial infarction via homeostasis of calcitonin gene-related peptide in myocardium.
abstract: Electrical stimulation has been shown protection of brain, retina, optic nerves and pancreatic β-cells but the effect on cardio-protection is still unknown. Calcitonin gene-related peptide (CGRP) participates in the pathology of injury and protection of myocardium but whether or not electrical stimulation modulates endogenous CGRP is not clear. Male Sprague-Dawley rats were divided into 4 groups: (1) control group, without any treatment. (2) I/R group, animals were subjected to 30 min of myocardial ischemia followed by 60 min reperfusion. (3) NES+I/R group, non-excitatory electrical stimulation (NES) was commenced from 15 min before coronary artery occlusion till the end of reperfusion. (4) I/R+CGRP8-37 group, animals were given with CGRP8-37 (an antagonist of CGRP receptor, 10(-7) mol/L, 0.3 ml, i.v.) at 5 min before reperfusion without any electrical stimulation. The hemodynamics and electrocardiogram were monitored and recorded. Infarct size and troponin I were examined and CGRP expression in the myocardium and serum was analyzed. It was found that the infarct size and TnI were significantly reduced in NES+I/R group, by 45% and 58% respectively, accompanied by an obvious fall back of CGRP in myocardium, compared to I/R group (all p<0.05). Treatment with CGRP8-37 resulted in the same protection on myocardium as NES did. No significant difference in hemodynamics or ventricular tachycardia was detected among the groups (all p>0.05). It can be concluded that NES reduced the infarction size after acute myocardial ischemia and reperfusion, for which the underlying mechanism may be associated with modulation of endogenous CGRP in myocardium.
authors: Department of Anesthesia, Shanxi Medical University, 56 Xinjiannan Road, Taiyuan 030001, Shanxi, China.
authors: Zhi-Jia
authors: ZJ
authors: Guo
authors: Department of Anesthesia, Shanxi Medical University, 56 Xinjiannan Road, Taiyuan 030001, Shanxi, China; Department of Anesthesia, Second Hospital of Shanxi Medical University, 382 Wuyi Road, Taiyuan 030001, Shanxi, China; Key Laboratory of Cellular Physiology at Shanxi Medical University, National Education Commission, China. Electronic address: guozheng713@163.com.
authors: Zheng
authors: Z
authors: Guo
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D004558:Electric Stimulation; D004599:Electric Stimulation Therapy; D004562:Electrocardiography; D015870:Gene Expression; D006439:Hemodynamics; D006706:Homeostasis; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000235:genetics* / Q000378:metabolism; D019210:Troponin I / Q000235:genetics / Q000378:metabolism; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D010446:Peptide Fragments; D018015:Receptors, Calcitonin Gene-Related Peptide; D019210:Troponin I; C058636:calcitonin gene-related peptide (8-37); D015740:Calcitonin Gene-Related Peptide
keywords: Calcitonin gene-related peptide (CGRP); Infarct size; Myocardial ischemia and reperfusion; Non-excitatory electrical stimulation