title: Facile approach to observe and quantify the α(IIb)β3 integrin on a single-cell.
abstract: We report for the first time seeing and counting integrin α(IIb)β3 on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin α(IIb)β3 on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au24Peptide8), the number of integrin α(IIb)β3 can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin α(IIb)β3 on a single cell varies from 5.75 × 10(-17) to 9.11 × 10(-17) mol, because of the heteroexpression levels of α(IIb)β3 on individual cells.
authors: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing, 100049, China.
authors: Jiao
authors: J
authors: Zhai
authors: Yaling
authors: Y
authors: Wang
authors: Chao
authors: C
authors: Xu
authors: Lingna
authors: L
authors: Zheng
authors: Meng
authors: M
authors: Wang
authors: Weiyue
authors: W
authors: Feng
authors: Liang
authors: L
authors: Gao
authors: Lina
authors: L
authors: Zhao
authors: Ru
authors: R
authors: Liu
authors: Fuping
authors: F
authors: Gao
authors: Yuliang
authors: Y
authors: Zhao
authors: Zhifang
authors: Z
authors: Chai
authors: Xueyun
authors: X
authors: Gao
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D018613:Microscopy, Confocal; D010446:Peptide Fragments / Q000032:analysis*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis*; D059010:Single-Cell Analysis / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D010446:Peptide Fragments; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D006046:Gold