title: B-1 cells produce insulin and abrogate experimental streptozotocin-induced diabetes.
abstract: The participation of B-1 cells in a murine model of spontaneous diabetes has been recently reported. Here, we describe the role of B-1 cells in streptozotocin (STZ) induced diabetes in mice. We demonstrated that XID (B-1 cell-deficient) mice are more susceptible to STZ treatment than WT mice, as evidenced by their higher blood glucose level in response to STZ. Unexpectedly, the XID mice that were i.p. transferred with purified B-1 cells, either before or after the STZ treatment, did not develop diabetes. These cell transfers provided long-lasting protection for the XID mice against STZ-induced diabetes, suggesting that B-1 cells play an important role in the experimental diabetes pathobiology. We also showed that B-1 cell culture supernatants were able to regulate the blood glucose level of the diabetic XID mice, and we identified insulin-producing cells when B-1 cells were differentiated in B-1 cell-derived phagocyte in vitro. These findings provide a novel role for B-1 cells in metabolic processes, presenting a new mechanism to explain the pathogenesis of diabetes and a possible therapeutical target.
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.|Programa de Pós Graduação em Patologia Ambiental e Experimental, Universidade Paulista-UNIP, São Paulo, Brasil.
authors: Anuska M
authors: AM
authors: Alvares-Saraiva
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.
authors: Marília C T
authors: MC
authors: Novo
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.
authors: Vivian Cristina
authors: VC
authors: de Oliveira
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.
authors: Juliana T
authors: JT
authors: Maricato
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.
authors: José Daniel
authors: JD
authors: Lopes
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.
authors: Ana Flavia
authors: AF
authors: Popi
authors: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brasil.|Programa de Pós Graduação em Patologia Ambiental e Experimental, Universidade Paulista-UNIP, São Paulo, Brasil.
authors: Mario
authors: M
authors: Mariano
mesh_terms: D019264:Adoptive Transfer; D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D007328:Insulin / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology; D013311:Streptozocin / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625951:Btk protein, mouse
keywords: B-1 cells; Experimental diabetes; Insulin; Streptozotocin; XID mice