title: Green synthesis of curcumin conjugated nanosilver for the applications in nucleic acid sensing and anti-bacterial activity.
abstract: Silver nanoparticles (Ag NPs) are often synthesized by chemical and physical methods. Natural and non-toxic molecules are recently being replaced for nanoparticles preparation. In this paper we have used curcumin, which interacts with Ag+ and subsequently synthesizes silver nanoparticles. Further continuation of the reaction often makes aggregation and forms dark brown/black silver oxide. Presence of glycerol in the reaction mixture gives mono-disperse curcumin conjugated Ag NPs, which can be made stable by capping with polyvinylpyrolidone (PVP). XRD data confirm that curcumin conjugated Ag NPs are crystalline in nature with a mean crystalline size of 13.27 nm. The Ag NPs are spherical and in the range of 10-50 nm though their hydrodynamic radius is found to be higher, ∼294 nm, due to polyvinylpyrolidone capping and aggregation of nanoparticles in solution. The production of curcumin conjugated Ag NPs follows first order kinetics and the effect of curcumin concentration during formation of Ag NPs indicates a linear enhancement in the production of Ag NPs with an increase in concentration of curcumin. These curcumin conjugated silver nanoparticles show anti-bacterial activity and can successfully determine nucleic acid (DNA and RNA) in the concentration range 100-1000 ng/mL with a linear regression coefficient >0.997 using Resonance Rayleigh Scattering spectra.
authors: Department of Chemistry, American University of Beirut, Beirut, Lebanon.
authors: Elsy
authors: E
authors: El Khoury
authors: Department of Nutrition and Food Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut, Lebanon.
authors: Mohamad
authors: M
authors: Abiad
authors: Department of Nutrition and Food Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut, Lebanon.
authors: Zeina G
authors: ZG
authors: Kassaify
authors: Department of Chemistry, American University of Beirut, Beirut, Lebanon. Electronic address: dp03@aub.edu.lb.
authors: Digambara
authors: D
authors: Patra
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D003116:Color; D003474:Curcumin / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D055772:Green Chemistry Technology / Q000379:methods*; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D009696:Nucleic Acids / Q000032:analysis*; D010316:Particle Size; D012542:Scattering, Radiation; D012834:Silver / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D009696:Nucleic Acids; D012834:Silver; D003474:Curcumin
keywords: Ag NPs; Anti-bacterial properties; Curcumin; DNA sensing; Green synthetic route