title: Local administration of calcitriol positively influences bone remodeling and maturation during restoration of mandibular bone defects in rats.
abstract: The aim of this study was to investigate the influence of calcitriol on osteoinduction following local administration into mandibular bone defects. Calcitriol-loaded absorbable collagen membrane scaffolds were prepared using the polydopamine coating method and characterized by scanning electron microscopy. Composite scaffolds were implanted into rat mandibular bone defects in the following groups: no graft material (control), bare collagen membrane (CM group), collagen membrane bearing polydopamine coating (DOP/CM group), and collagen membrane bearing polydopamine coating absorbed with calcitriol (CAL/DOP/CM group). At 1, 2, 4 and 8weeks post-surgery, the osteogenic potential of calcitriol was examined by histological and immunohistochemical methods. Following in vivo implantation, calcitriol-loaded composite scaffolds underwent rapid degradation with pronounced replacement by new bone and induced reunion of the bone marrow cavity. Calcitriol showed strong potential in inhibiting osteoclastogenesis and promotion of osteogenic differentiation at weeks 1, and 2. Furthermore, statistical analysis revealed that the newly formed bone volume in the CAL/DOP/CM group was significantly higher than other groups at weeks 1, and 2. At weeks 4, and 8, the CAL/DOP/CM group showed more mineralized bone and uniform collagen structure. These data suggest that local administration of calcitriol is promising in promoting osteogenesis and mineralization for restoration of mandibular bone defects.
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Hongrui
authors: H
authors: Liu
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Jian
authors: J
authors: Cui
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Wei
authors: W
authors: Feng
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Shengyu
authors: S
authors: Lv
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Juan
authors: J
authors: Du
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Jing
authors: J
authors: Sun
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Xiuchun
authors: X
authors: Han
authors: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
authors: Zhenming
authors: Z
authors: Wang
authors: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
authors: Xiong
authors: X
authors: Lu
authors: Department of Advanced Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors: Yimin
authors: Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Kimimitsu
authors: K
authors: Oda
authors: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors: Norio
authors: N
authors: Amizuka
authors: Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China. Electronic address: liminqi@sdu.edu.cn.
authors: Minqi
authors: M
authors: Li
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D002117:Calcitriol / Q000008:administration & dosage* / Q000494:pharmacology; D008334:Mandible / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds
chemical_list: D002117:Calcitriol
keywords: Calcitriol; Mandibular bone defect; Mineralization; Osteoblast; Osteoclast