title: Dietary squid ink polysaccharide induces goblet cells to protect small intestine from chemotherapy induced injury.
abstract: Gastrointestinal mucositis induced by chemotherapy is associated with alterations of intestinal barrier function due to the potential damage induced by anti-cancer drugs on the epithelial cells. Goblet cells, an important epithelial lining in the intestine, contribute to innate immunity by secreting mucin glycoproteins. Employing a mouse model of chemotherapy induced intestinal mucosal immunity injury by cyclophosphamide, we demonstrated for the first time that polysaccharide from the ink of Ommastrephes bartramii (OBP) enhanced Cyto18, which is a mucin expression in goblet cells. The up-regulation of mucins by OBP relied on the augmented quantity of goblet cells, but not on the changes in the ultrastructure of endoplasmic reticulum (ER). Our results may have important implications for enhanced immunopotentiation function of functional OBP on intestinal mucosal immunity against intestinal disorders involving inflammation and infection.
authors: College of Food Science and Engineering, Ocean University of China, Yushan Road 5th, Qingdao, Shandong province 266003, PR China. tangqingjuan@ouc.edu.cn ouczt@hotmail.com.
authors: Tao
authors: T
authors: Zuo
authors: Lu
authors: L
authors: Cao
authors: Changhu
authors: C
authors: Xue
authors: Qing-Juan
authors: QJ
authors: Tang
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D012634:Bodily Secretions / Q000737:chemistry*; D003520:Cyclophosphamide / Q000009:adverse effects; D049832:Decapodiformes / Q000502:physiology*; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007113:Immunity, Innate / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D053538:Keratin-18 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D055262:Mucin-2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D052016:Mucositis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011134:Polysaccharides / Q000302:isolation & purification / Q000627:therapeutic use*; D020011:Protective Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D053538:Keratin-18; D055262:Mucin-2; D011134:Polysaccharides; D020011:Protective Agents; D003520:Cyclophosphamide