title: Acute and subchronic toxicity as well as evaluation of safety pharmacology of eucalyptus oil-water emulsions.
abstract: Essential oil has performed a variety of indirect services used as insect/pest repellent. The present study investigated the acute and subchronic toxicity of eucalyptus oil emulsion in water (EOE). In addition, we conduct safety pharmacology evaluation of EOE to supplement the toxicity tests and provide a basis for a comprehensive understanding of the toxicity of EOE. Acute administration of EOE was done as single dose from 2772 mg to 5742 mg of EOE per kg/bodyweight (b.wt.) and subchronic toxicity study for thirty days was done by daily oral administration of EOE at doses of 396, 792 and 1188 mg/kg b.wt. In SPF SD rats. The acute toxicity study showed the LD50 of EOE was 3811.5 mg/kg. The subchronic toxicity study suggested the high-dose and middle-dose EOE slowed down the growth of male rats. The clinical pathology showed the high-dose and middle-dose EOE could cause damage to liver and kidney. The safety pharmacology indicated that EOE had no side effects on rats. These results suggest that EOE is a safe veterinary medicine for external use.
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Zhiqiang
authors: Z
authors: Hu
authors: Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education Yi'bin 644000, China.
authors: Ruizhang
authors: R
authors: Feng
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Fa
authors: F
authors: Xiang
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Xu
authors: X
authors: Song
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Zhongqiong
authors: Z
authors: Yin
authors: Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education Yi'bin 644000, China.
authors: Chao
authors: C
authors: Zhang
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Xinghong
authors: X
authors: Zhao
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Renyong
authors: R
authors: Jia
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Zhenzhen
authors: Z
authors: Chen
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Li
authors: L
authors: Li
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Lizi
authors: L
authors: Yin
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Xiaoxia
authors: X
authors: Liang
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Changliang
authors: C
authors: He
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Gang
authors: G
authors: Shu
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Cheng
authors: C
authors: Lv
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Ling
authors: L
authors: Zhao
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Gang
authors: G
authors: Ye
authors: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors: Fei
authors: F
authors: Shi
keywords: Essential oil; acute toxicity; safety pharmacology; subchronic toxicity