title: Genome characterization of a GII.6 norovirus strain identified in China.
abstract: Noroviruses (NoVs) are the primary cause of non-bacterial acute gastroenteritis worldwide. Most NoV infections are caused by GII.4, but GII.6 is also an important genotype with a long-term persistence in human populations. In this study, the complete genome sequence of a NoV strain GZ2010-L96 isolated in China was identified and analyzed phylogenetically. The viral genome comprised 7550 nucleotides, and its phylogenetic analysis revealed that the strain belonged to GII.6 genotype. All reported GII.6 NoV capsid protein sequences were also collected for comparative analysis, and GZ2010-L96 was clustered into GII.6-b with other 8 strains. Meanwhile, it was found that 53 spots on viral capsid showed subcluster specificity according to multiple alignments. Moreover, homologous modeling of GZ2010-L96 based on comparison with GII.4 VA387 strain showed a different antigen distribution pattern. In summary, the genome of the GII.6 strain GZ2010-L96 detected in China was extensively characterized, and phylogenetic analyses of GII.6 NoVs based on the capsid proteins may reveal a different evolution process from the predominant genotype GII.4.
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors: Liang
authors: L
authors: Xue
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China. Electronic address: wuqp203@163.com.
authors: Qingping
authors: Q
authors: Wu
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors: Xiaoxia
authors: X
authors: Kou
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors: Weicheng
authors: W
authors: Cai
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors: Jumei
authors: J
authors: Zhang
authors: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
authors: Weipeng
authors: W
authors: Guo
mesh_terms: D000595:Amino Acid Sequence; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002681:China / Q000453:epidemiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D011159:Population Surveillance; D011487:Protein Conformation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins
keywords: Comparative analysis; Complete genome; GII.6; Homology modeling; Norovirus; Phylogenetic tree