title: Discrimination between alternative substrates and inhibitors of tyrosinase.
abstract: Many phenolic compounds have been described in the scientific literature as inhibitors of tyrosinase. In this work a test is proposed that allows us to distinguish whether a molecule is an enzyme inhibitor or substrate. The test has several stages. First, the degree of inhibition of the studied molecule is determined on the monophenolase activity (i(M)) and on the diphenolase activity (i(D)). If i(M) = i(D), it is an inhibitor. If i(M) ≠ i(D), the molecule could be substrate or inhibitor. Several additional stages are proposed to solve this ambiguity. The study described herein was carried out using the following molecules: benzoic acid, cinnamic acid, guaiacol, isoeugenol, carvacrol, 4-tert-butylphenol, eugenol, and arbutin.
authors: GENZ-Group of Research on Enzymology, Department of Biochemistry and Molecular Biology-A, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia , 0100 Espinardo, Murcia, Spain.
authors: Carmen Vanessa
authors: CV
authors: Ortiz-Ruiz
authors: Maria del Mar
authors: Mdel M
authors: Garcia-Molina
authors: Jose Tudela
authors: JT
authors: Serrano
authors: Virginia
authors: V
authors: Tomas-Martinez
authors: Francisco
authors: F
authors: Garcia-Canovas
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D016002:Discriminant Analysis; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D010636:Phenols; D014442:Monophenol Monooxygenase
keywords: degree of inhibition; inhibitor; kinetic; polyphenol oxidase; substrate; tyrosinase