title: MicroRNA-494 inhibits the growth and angiogenesis-regulating potential of mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) play an important role in the pathology of preeclampsia (PE). Our previous microarray analysis found that microRNA-494 (miR-494) is highly expressed in decidua-derived MSCs (dMSCs) from PE. We hypothesized that aberrant expression of miR-494 in dMSCs is involved in PE development. In the present study, we found that miR-494 arrests G1/S transition in dMSCs by targeting CDK6 and CCND1. We also found that supernatant from miR-494-overexpressing dMSCs reduces HTR-8/SVneo migration and impairs HUVEC capillary formation by suppressing VEGF. Taken together, we report an unrecognized mechanism of miR-494 affecting dMSC proliferation and function in the pathology of PE.
authors: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors: Shiwen
authors: S
authors: Chen
authors: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors: Guangfeng
authors: G
authors: Zhao
authors: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors: Huishuang
authors: H
authors: Miao
authors: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors: Ruijing
authors: R
authors: Tang
authors: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors: Yuxian
authors: Y
authors: Song
authors: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
authors: Yali
authors: Y
authors: Hu
authors: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China. Electronic address: 13815885457@163.com.
authors: Zhiqun
authors: Z
authors: Wang
authors: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China. Electronic address: yayihou@nju.edu.cn.
authors: Yayi
authors: Y
authors: Hou
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D003656:Decidua / Q000473:pathology; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D018919:Neovascularization, Physiologic; D011225:Pre-Eclampsia / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D034622:RNA Interference; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C547168:MIRN494 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Angiogenesis; Mesenchymal stem cell; Preeclampsia; Vascular endothelial growth factor; microRNA-494-3p