title: [Activation of transient receptor potential vanilloid subtype 1 serves as a novel pathway to modulate secretion in submandibular gland].
abstract: Our work focused on the studies on the expression and function of transient receptor potential vanilloid subtype 1 (TRPV1) in the submandibular gland. By using reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunofluorescence, our data demonstrated the expression and distribution characteristics of TRPV1 in rabbit and human submandibular glands, as well as rat submandibular gland cell line SMG-C6. Furthermore, the possible intracellular signal molecules involved in the TRPV1-modulated saliva secretion were explored. Activation of TRPV1 increased the intracellular Ca(2+) concentration, upregulated the expression of aquaporin 5 (AQP5), the main transporter that mediate water secretion through transcellular pathway, and led to AQP5 redistribution. Extracellular signal-regulated kinase 1/2 (ERK1/2) was involved in the TRPV1-regulated AQP5 content. Besides, TRPV1 activation also modulated the expression, distribution, and function of tight junction protein, and increased paracellular permeability. ERK1/2 and myosin light chain 2 (MLC2) were responsible for the regulation of TRPV1on tight junction properties. Taken together, our work suggested that TRPV1 was a potential target to promote saliva secretion, and activation of TRPV1 might provide a new and safe therapeutic strategy to ameliorate submandibular gland hypofunction.
authors: Center for Salivary Gland Diseases, Peking University School and Hospital of Stomatology, Beijing 100081, China; Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors: Xin
authors: X
authors: Cong
authors: Center for Salivary Gland Diseases, Peking University School and Hospital of Stomatology, Beijing 100081, China; Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors: Yan
authors: Y
authors: Zhang
authors: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Guang-yan
authors: GY
authors: Yu
authors: Center for Salivary Gland Diseases, Peking University School and Hospital of Stomatology, Beijing 100081, China; Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors: Li-ling
authors: LL
authors: Wu
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism; D024722:Cardiac Myosins / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000378:metabolism; D012472:Salivation; D013363:Submandibular Gland / Q000502:physiology*; D050916:TRPV Cation Channels / Q000502:physiology*; D019108:Tight Junctions / Q000502:physiology; D015854:Up-Regulation
chemical_list: D051402:Aquaporin 5; D018994:Myosin Light Chains; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C107184:myosin light chain 2; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D024722:Cardiac Myosins