title: Microbial community dynamics associated with veterinary antibiotics removal in constructed wetlands microcosms.
abstract: This study aimed to evaluate the response of the microbial community from CWs microcosms tested for the removal of two veterinary antibiotics, enrofloxacin (ENR) and tetracycline (TET), from livestock industry wastewater. Three treatments were tested (control, ENR or TET (100 μg L(-1))) over 12 weeks in microcosms unplanted and planted with Phragmites australis. CWs removal efficiency was relatively stable along time, with removals higher than 98% for ENR and 94% for TET. In addition, CWs were able to reduce wastewater toxicity, independently of antibiotics presence. Despite no significant differences were observed in terms of microbial abundance, bacterial richness or diversity, analysis of similarities (two-way crossed ANOSIM) showed a significant effect of both time and treatments in bacterial community structure. This study points to CWs applicability for veterinary antibiotics removal from livestock wastewaters, showing that CWs microbial communities were able to adapt without significant changes in their diversity or depuration capacity.
authors: Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal; Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
authors: Joana P
authors: JP
authors: Fernandes
authors: Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
authors: C Marisa R
authors: CMR
authors: Almeida
authors: Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
authors: Ana C
authors: AC
authors: Pereira
authors: Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
authors: Iolanda L
authors: IL
authors: Ribeiro
authors: Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
authors: Izabela
authors: I
authors: Reis
authors: Department of Bioscience, Aarhus University, Ole Worms Allé 1, Building 1135, 8000 Aarhus C., Denmark.
authors: Pedro
authors: P
authors: Carvalho
authors: Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal; Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
authors: M Clara P
authors: MCP
authors: Basto
authors: Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. Electronic address: amucha@ciimar.up.pt.
authors: Ana P
authors: AP
authors: Mucha
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001673:Biodegradation, Environmental; D044822:Biodiversity; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000378:metabolism; D059013:Microbial Consortia / Q000187:drug effects; D006109:Poaceae; D013752:Tetracycline / Q000378:metabolism / Q000494:pharmacology; D019155:Veterinary Drugs / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D062065:Wastewater / Q000737:chemistry / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*; D053833:Wetlands
chemical_list: D000900:Anti-Bacterial Agents; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D013752:Tetracycline; D019155:Veterinary Drugs; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Constructed wetlands; Microbial community; Veterinary antibiotics