title: Tenellin acts as an iron chelator to prevent iron-generated reactive oxygen species toxicity in the entomopathogenic fungus Beauveria bassiana.
abstract: Iron is an essential element for life. However, the iron overload can be toxic. Here, we investigated the significant increase of tenellin and iron-tenellin complex production in ferricrocin-deficient mutants of Beauveria bassiana. Our chemical analysis indicated that the ferricrocin-deficient mutants T1, T3 and T5 nearly abolished ferricrocin production. In turn, these mutants had significant accumulation of iron-tenellin complex in their mycelia at 247-289 mg g(-1) cell dry weight under iron-replete condition. Both tenellin and iron-tenellin complex were not detected in the wild-type under such condition. Mass analysis of the mutants' crude extracts demonstrated that tenellin formed a 3:1 complex with iron in the absence of ferricrocin. The unexpected link between ferricrocin and tenellin biosynthesis in ferricrocin-deficient mutants could be a survival strategy during iron-mediated oxidative stress.
authors: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
authors: Jiraporn
authors: J
authors: Jirakkakul
authors: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
authors: Supapon
authors: S
authors: Cheevadhanarak
authors: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors: Juntira
authors: J
authors: Punya
authors: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors: Chanikul
authors: C
authors: Chutrakul
authors: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors: Jittisak
authors: J
authors: Senachak
authors: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors: Taridaporn
authors: T
authors: Buajarern
authors: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
authors: Morakot
authors: M
authors: Tanticharoen
authors: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand alongkorn@biotec.or.th.
authors: Alongkorn
authors: A
authors: Amnuaykanjanasin
mesh_terms: D052982:Beauveria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D005291:Ferrichrome / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D013058:Mass Spectrometry; D009154:Mutation; D011728:Pyridones / Q000737:chemistry / Q000378:metabolism*; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*
chemical_list: D011728:Pyridones; D017382:Reactive Oxygen Species; D017262:Siderophores; D005291:Ferrichrome; C021678:ferricrocin; C015528:tenellin; D007501:Iron
keywords: Beauveria bassiana; ferricrocin; intracellular siderophore; iron chelator; iron-generated reactive oxygen species; tenellin