title: Micromonospora costi sp. nov., isolated from a leaf of Costus speciosus.
abstract: An endophytic actinobacterial strain, CS1-12(T), was isolated from a leaf of Costus speciosus. Single spores were observed directly on the substrate mycelia. The cell-wall peptidoglycan of this strain exhibited meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid. Arabinose, glucose, ribose, xylose and rhamnose were detected as whole-cell sugars. The diagnostic phospholipids of this strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant menaquinones were MK-10(H6), MK-10(H8), MK-9(H4) and MK-10(H4). The main components of the cellular fatty acids (>10%) were iso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 72.8 mol%. 16S rRNA gene sequence analysis data showed that strain CS1-12(T) should be classified in the genus Micromonospora and that it is closely related to Micromonospora fulviviridis DSM 43906(T) (99.3%). The physiological, biochemical and chemotaxonomic properties, including the DNA-DNA hybridization results, indicated that strain CS1-12(T) could be judged a novel species of the genus Micromonospora , for which the name Micromonospora costi sp. nov. is proposed. The type strain is CS1-12(T) ( =BCC 58124(T) =NBRC 109518(T)).
authors: Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.|Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors: Chitti
authors: C
authors: Thawai
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D029770:Costus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid