title: hiPS-MSCs differentiation towards fibroblasts on a 3D ECM mimicking scaffold.
abstract: Fibroblasts are ubiquitous cells that constitute the stroma of virtually all tissues and play vital roles in homeostasis. The poor innate healing capacity of fibroblastic tissues is attributed to the scarcity of fibroblasts as collagen-producing cells. In this study, we have developed a functional ECM mimicking scaffold that is capable to supply spatial allocation of stem cells as well as anchorage and storage of growth factors (GFs) to direct stem cells differentiate towards fibroblasts. Electrospun PCL fibers were embedded in a PEG-fibrinogen (PF) hydrogel, which was infiltrated with connective tissue growth factor (CTGF) to form the 3D nanocomposite PFP-C. The human induced pluripotent stem cells derived mesenchymal stem cells (hiPS-MSCs) with an advance in growth over adult MSCs were applied to validate the fibrogenic capacity of the 3D nanocomposite scaffold. The PFP-C scaffold was found not only biocompatible with the hiPS-MSCs, but also presented intriguingly strong fibroblastic commitments, to an extent comparable to the positive control, tissue culture plastic surfaces (TCP) timely refreshed with 100% CTGF. The novel scaffold presented not only biomimetic ECM nanostructures for homing stem cells, but also sufficient cell-approachable bio-signaling cues, which may synergistically facilitate the control of stem cell fates for regenerative therapies.
authors: interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
authors: Ruodan
authors: R
authors: Xu
authors: interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
authors: Mehmet Berat
authors: MB
authors: Taskin
authors: interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
authors: Marina
authors: M
authors: Rubert
authors: 1] Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel [2] The Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors: Dror
authors: D
authors: Seliktar
authors: interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
authors: Flemming
authors: F
authors: Besenbacher
authors: interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
authors: Menglin
authors: M
authors: Chen
mesh_terms: D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D053761:Nanocomposites; D010641:Phenotype; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D055513:Connective Tissue Growth Factor