title: [The distribution of MAP kinase phosphatase-1 in the cerebrospinal fluid-contacting nucleus and its functional contribution to depressive behaviors].
abstract: The purpose of this research is to explore the distribution and expression of MAP kinase phosphatase-1 (MKP-1) in cerebrospinal fluid (CSF)-contacting nucleus in depression, and provide experimental evidence to reveal the biological function and regulatory mechanisms of CSF-contacting nucleus in depression. Depression model was produced by chronic forced swimming stress (CFSS) in Sprague-Dawley (SD) rats. Intracerebroventricular injection of cholera toxin subunit B (CTb) labeled with horseradish peroxidase (CB-HRP) was used to specifically mark distal CSF-contacting nucleus. The rate of animal growth and behavioral tests including sucrose preference test (SPT) and open field test (OFT) were used to validate the model of depression. The expressions of MKP-1 and fos proteins in CSF-contacting nucleus were detected by immunofluorescence. Software Image-Pro Plus version 6.0 was used to count the positive neurons. The results showed that, the distributions of MKP-1 were found in the CSF-contacting nucleus. After 28 days of swimming, the rats in stress group had a lower growth rate, a less consumption of sucrose and lower scores of OFT compared to control group. The number of neurons double labeled with CB-HRP/fos or CB-HRP/MKP-1 in stress group was significantly higher than that in control group (P < 0.01). These results suggest that the CSF-contacting nucleus may be involved in the process of depression via the MKP-1.
authors: Department of Anesthesiology, First Affiliated Hospital, Fujian Medical University, Fuzhou 350000, China.|Jiangsu Province Key Laboratory of Anaesthesiology, Xuzhou Medical College, Xuzhou 221009, China.
authors: Ping
authors: P
authors: Chen
authors: Department of Neurosurgery, First Affiliated Hospital, Fujian Medical University, Fuzhou 350000, China.
authors: Qing-Song
authors: QS
authors: Lin
authors: Jiangsu Province Key Laboratory of Anaesthesiology, Xuzhou Medical College, Xuzhou 221009, China. lczhang@xzmc.edu.cn.
authors: Li-Cai
authors: LC
authors: Zhang
mesh_terms: D000818:Animals; D002555:Cerebrospinal Fluid; D003863:Depression / Q000201:enzymology*; D054638:Dual Specificity Phosphatase 1 / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological
chemical_list: D054638:Dual Specificity Phosphatase 1