title: Saposin-like protein 2 has an immunodiagnostic potential for detecting Fasciolosis gigantica.
abstract: Saposin-like protein 2 (SAP-2) plays an important role in the digestive process of Fasciola gigantica (Fg). It is one of the major proteins synthesized by the caecal epithelial cells and released into fluke's excretion-secretion. Therefore, FgSAP-2 is a plausible target for detecting fasciolosis. A polyclonal antibody (PoAb) against recombinant FgSAP-2 was produced by immunizing rabbits with the recombinant protein (rFgSAP-2), and used in sandwich ELISA assay to detect the circulating FgSAP-2 in sera of mice experimentally infected with F. gigantica metacercariae. The assay could detect rFgSAP-2 and the native FgSAP-2 in the excretory-secretory (ES) and whole body (WB) fractions of adult F. gigantica at the concentrations as low as 38 pg/ml, 24 ng/ml, and 102 ng/ml, respectively. As well, the sera from mice experimentally infected with F. gigantica were tested positive by this sandwich ELISA, which exhibited sensitivity, specificity, false positive rate, false negative rate and accuracy at 99.99, 98.67, 1.33, 0.01 and 99.32%, respectively. Therefore, this assay could be used for diagnosis of fasciolosis by F. gigantica.
authors: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Rd, Saen Sook Sub-district, Mueang District, Chonburi 20131, Thailand.
authors: Pornanan
authors: P
authors: Kueakhai
authors: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Rd, Saen Sook Sub-district, Mueang District, Chonburi 20131, Thailand.
authors: Narin
authors: N
authors: Changklungmoa
authors: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors: Kulathida
authors: K
authors: Chaithirayanon
authors: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 52000, Thailand.
authors: Manussabhorn
authors: M
authors: Phatsara
authors: Faculty of Animal Science, Silpakorn University, Phetchaburi Campus, Cha-am, Phetchaburi 76120, Thailand.
authors: Narin
authors: N
authors: Preyavichyapugdee
authors: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors: Suda
authors: S
authors: Riengrojpitak
authors: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors: Kant
authors: K
authors: Sangpairoj
authors: Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Thailand.
authors: Phiraphol
authors: P
authors: Chusongsang
authors: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand. Electronic address: prasert.sob@mahidol.ac.th.
authors: Prasert
authors: P
authors: Sobhon
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005209:Fasciola / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D008297:Male; D051379:Mice; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D049231:Saposins / Q000276:immunology / Q000378:metabolism; D012552:Schistosomiasis / Q000097:blood / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D049231:Saposins
keywords: Fasciola gigantica; Immunodiagnosis; Sandwich ELISA; Saposin-like protein 2