title: Effect of different calcium phosphate scaffold ratios on odontogenic differentiation of human dental pulp cells.
abstract: Calcium phosphate (CaP) scaffolds have been widely and successfully used with osteoblast cells for bone tissue regeneration. However, it is necessary to investigate the effects of these scaffolds on odontoblast cells' proliferation and differentiation for dentin tissue regeneration. In this study, three different hydroxyapatite (HA) to beta tricalcium phosphate (β-TCP) ratios of biphasic calcium phosphate (BCP) scaffolds, BCP20, BCP50, and BCP80, with a mean pore size of 300μm and 65% porosity were prepared from phosphoric acid (H2PO4) and calcium carbonate (CaCO3) sintered at 1000°C for 2h. The extracts of these scaffolds were assessed with regard to cell viability and differentiation of odontoblasts. The high alkalinity, more calcium, and phosphate ions released that were exhibited by BCP20 decreased the viability of human dental pulp cells (HDPCs) as compared to BCP50 and BCP80. However, the cells cultured with BCP20 extract expressed high alkaline phosphatase activity and high expression level of bone sialoprotein (BSP), dental matrix protein-1 (DMP-1), and dentin sialophosphoprotein (DSPP) genes as compared to that cultured with BCP50 and BCP80 extracts. The results highlighted the effect of different scaffold ratios on the cell microenvironment and demonstrated that BCP20 scaffold can support HDPC differentiation for dentin tissue regeneration.
authors: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Department of Pedodontic and Preventive Dentistry, College of Dentistry, University of Baghdad, Baghdad, Iraq.
authors: Sarah Talib
authors: ST
authors: AbdulQader
authors: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Electronic address: kannan@usm.my.
authors: Thirumulu Ponnuraj
authors: TP
authors: Kannan
authors: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors: Ismail Ab
authors: IA
authors: Rahman
authors: School of Materials and Minerals Resource Engineering, Universiti Sains Malaysia, 14300 Penang, Malaysia.
authors: Hanafi
authors: H
authors: Ismail
authors: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors: Zuliani
authors: Z
authors: Mahmood
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D003804:Dentin / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D009804:Odontoblasts / Q000187:drug effects / Q000378:metabolism; D009805:Odontogenesis / Q000187:drug effects* / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D012795:Sialoglycoproteins / Q000378:metabolism; D054457:Tissue Scaffolds; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D002130:Calcium Phosphates; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C485817:beta-tricalcium phosphate; C020243:calcium phosphate
keywords: Biphasic calcium phosphate; Calcium and phosphate ions; Human dental pulp cells; Odontogenesis; Tissue regeneration