title: Preparation of multi-enzyme co-immobilized nanoparticles by bis-aryl hydrazone bond conjugation.
abstract: A novel multi-enzyme co-immobilized nanoparticle was prepared by the bis-aryl hydrazone conjugation strategy for enhancing the overall reaction efficiency and specificity. It can be seen that the molar substitution ratios of succinimidyl 6-hydrazinonicotinamid acetone hydrazone or succinimidyl 4-formylbenzoate to glucose oxidase (GOX) or horseradish peroxidase (HRP) increased with the concentration of the linking reagents. The amount of the immobilized conjugates on the support was measured to be 22.8 ± 1.6 mg/g-particle, and it meant that more than 90% of the GOX-HRP conjugates were successfully attached onto the polystyrene (PS) nanoparticles. Moreover, the immobilized bi-enzymes conjugate on the PS nanoparticles increased about 1.6-fold compared with that of free enzymes.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, Peoples' Republic of China.
authors: Xiao
authors: X
authors: Zhou
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, Peoples' Republic of China.
authors: Yan
authors: Y
authors: Liu
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, Peoples' Republic of China.
authors: Qipeng
authors: Q
authors: Yuan
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, Peoples' Republic of China.
authors: Hao
authors: H
authors: Liang
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006835:Hydrazones / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D006835:Hydrazones; D011137:Polystyrenes; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase
keywords: bioconjugation; bis-aryl hydrazone; glucose oxidase; horseradish peroxidase; multi-enzyme immobilization nanoparticles