title: Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp.
abstract: In this study, the crude polysaccharide (APPS) from the fruiting bodies of apricot (Armeniaca sibirica L. Lam.) was isolated and fractionated by ultrafiltration and Sephadex G-75 gel chromatography. The hypoglycemic activities of all fractions were determined by α-glucosidase inhibitory activity in vitro. The fraction APPS1-2 showed the best activity with an IC50 of 6.06 mg/mL. The properties and chemical compositions of this fraction were analyzed with high-performance liquid chromatography, gel permeation chromatography-eighteen angle laser light scattering instrument, UV spectroscopy, infrared spectroscopy, and NMR spectroscopy ((1)H). The results demonstrated that APPS1-2 was a neutral glycoconjugate with a molecular weight of 25.93 kDa. It comprised rhamnose, glucose, mannose, and galactose, with a relative molar ratio of 1.34:2.01:0.48:0.35. The backbone of APPS1-2 may consist of rhamnose and glucose, but its branches may consist of mannose and galactose. The IR and UV spectrum of APPS1-2 revealed the typical characteristics of heteropolysaccharide. (1)H NMR spectrum showed that APPS1-2 contained α-configurations.
authors: College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, PR China.
authors: Jie
authors: J
authors: Cui
authors: College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, PR China.
authors: Xin
authors: X
authors: Gu
authors: College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, PR China.
authors: Fengjun
authors: F
authors: Wang
authors: College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, PR China.
authors: Jie
authors: J
authors: Ouyang
authors: College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, PR China. Electronic address: w62338221@163.com.
authors: Jianzhong
authors: J
authors: Wang
mesh_terms: D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D027861:Prunus / Q000737:chemistry*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Anthrone (PubChem CID: 7018); Apricot polysaccharide; Structural analysis; Sulfuric acid (PubChem CID: 1118); d-Galactose (PubChem CID: 6036); d-Glucose (PubChem CID: 5793); d-Mannose (PubChem CID: 18950); d-Xylose (PubChem CID: 135191); l-Rhamnose (PubChem CID: 25310); p-Nitrophenyl-α-d-glucopyranoside (PubChem CID: 259380); α-Glucosidase inhibitor