title: Chemopreventive potential of the tannase-mediated biotransformation of green tea.
abstract: Green tea (Camellia sinensis) is one of the most widely consumed beverages in the world. The cancer chemopreventive qualities of green tea have been well documented. Epigallocatechin gallate (EGCG) is often described as the most potently chemopreventive green tea catechin; however, the low bioavailability of EGCG is a limiting factor for its biological effect. Thus, the aim of this work was to test the chemopreventive potential of green tea extract and EGCG after tannase-mediated hydrolysis. The results showed that the biotransformed compounds retained most of the beneficial properties of the original compounds, and some beneficial properties were improved in the biotransformed compounds. Biotransformation of EGCG decreased its toxicity without affecting its antiproliferative effects. Furthermore, human cells gene expression profiling showed that the biotransformed compounds modulated the expression of several genes related to carcinogenesis. These results demonstrate the benefits of the biotechnological modification of natural food molecules, allowing the improvement of the nutraceutical potential of a beverage as green tea.
authors: Food Science Department, Faculty of Food Engineering, Campinas State University, P.O. Box 6121, 13083-862 SP, Brazil; Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG), São Francisco University, Av. São Francisco de Assis 218, Bragança Paulista, SP, Brazil. Electronic address: jumacedo@gmail.com.
authors: J A
authors: JA
authors: Macedo
authors: Food Science Department, Faculty of Food Engineering, Campinas State University, P.O. Box 6121, 13083-862 SP, Brazil.
authors: L R
authors: LR
authors: Ferreira
authors: Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG), São Francisco University, Av. São Francisco de Assis 218, Bragança Paulista, SP, Brazil.
authors: L E
authors: LE
authors: Camara
authors: Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG), São Francisco University, Av. São Francisco de Assis 218, Bragança Paulista, SP, Brazil.
authors: J C
authors: JC
authors: Santos
authors: Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG), São Francisco University, Av. São Francisco de Assis 218, Bragança Paulista, SP, Brazil.
authors: A
authors: A
authors: Gambero
authors: Food Science Department, Faculty of Food Engineering, Campinas State University, P.O. Box 6121, 13083-862 SP, Brazil.
authors: G A
authors: GA
authors: Macedo
authors: Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG), São Francisco University, Av. São Francisco de Assis 218, Bragança Paulista, SP, Brazil.
authors: M L
authors: ML
authors: Ribeiro
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001711:Biotransformation; D028241:Camellia sinensis / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D018890:Chemoprevention; D020552:Comet Assay / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D010936:Plant Extracts / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate; D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: Chemoprevention; Comet assay; Gene expression; Green tea; Tannase