title: Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer.
abstract: OBJECTIVE
  To investigate the interaction between Xiaotan Sanjie (XTSJ) decoction and interleukin-8 (IL-8) and its effect on adhesion, migration and invasion of SGC-7901 gastric cancer cells.
  
  
  METHODS
  SGC-7901 gastric cancer cells were exposed to serum containing XTSJ decoction and/or IL-8 (1 ng/mL). SGC-7901 cell adhesion to fibronectin, an extracellular matrix component, was detected using the Cell Counting Kit-8. Migration and invasion abilities of SGC-7901 cells were detected by scratch wound and Transwell chamber assays. Then, protein (immunofluorescence and Western blot) and mRNA levels (quantitative polymerase chain reaction) of cluster of differentiation 44 (CD44), a cell adhesion molecule, were measured in 72-h-cultured SGC-7901 cells.
  
  
  RESULTS
  Cell adhesion was promoted by IL-8 (P = 0.001), but was inhibited by XTSJ decoction (P = 0.0001). Similarly, IL-8 promoted SGC-7901 cell invasion (P = 0.003), and XTSJ decoction inhibited cell invasion (P = 0.001). IL-8 induced SGC-7901 cell migration, but this was inhibited by XTSJ decoction. IL-8 up-regulated CD44 protein (P = 0.028) and mRNA expression (P = 0.002), whereas XTSJ decoction inhibited CD44 protein expression (P = 0.0001), but not mRNA expression (P = 0.275). An interaction between XTSJ decoction and IL-8 was confirmed in the invasion (P = 0.001) and CD44 mRNA expression of SGC-7901 cells (P = 0.010), but not in cell adhesion (P = 0.051).
  
  
  CONCLUSIONS
  XTSJ decoction may inhibit adhesion, migration and invasion of gastric cancer cells, which is partly associated with down-regulation of IL-8.
authors: Jun Shi, Pin-Kang Wei, Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China.
authors: Jun
authors: J
authors: Shi
authors: Jun Shi, Pin-Kang Wei, Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China.
authors: Pin-Kang
authors: PK
authors: Wei
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000494:pharmacology*; D008297:Male; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C497483:CD44 protein, human; D004365:Drugs, Chinese Herbal; D018960:Hyaluronan Receptors; D016209:Interleukin-8; D012333:RNA, Messenger; C000598250:xiaotan sanjie
keywords: Adhesion; Cluster of differentiation 44; Gastric cancer SGC-7901 cell line; Interleukin-8; Invasion; Migration; Xiaotan Sanjie decoction