title: Predictive performance of the Short Time Exposure test for identifying eye irritation potential of chemical mixtures.
abstract: The Short Time Exposure (STE) test is an in vitro eye irritation test based on the cytotoxicity in SIRC cells (rabbit corneal cell line) following a 5 min treatment of chemicals. This study evaluated the predictive performance of the STE test to identify the globally harmonized system (GHS) Not Classified category and other irritant categories (i.e., GHS Category 1 or 2) when used to test 40 chemical mixtures that included irritants. The STE test correctly identified 30 tested mixtures classified as GHS irritant categories and 5 out of 10 tested mixtures classified as GHS Not Classified. The sensitivity, specificity, positive predictivity, negative predictivity, and overall accuracy of the STE test were 100% (30/30), 50% (5/10), 86% (25/30), 100% (5/5), and 88% (35/40), respectively. These predictive performances were comparative to or greater than those in other in vitro eye irritation tests that have been accepted as test guideline by the Organisation for Economic Co-operation and Development. This suggests that the STE test has sufficient predictivity for identifying the eye irritation potential of chemical mixtures. Since no false negatives in this study were found, this indicates that the STE test is applicable as a part of the bottom-up approach.
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan. Electronic address: saitou.kazutoshi@kao.co.jp.
authors: Kazutoshi
authors: K
authors: Saito
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.
authors: Masaaki
authors: M
authors: Miyazawa
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.
authors: Yuko
authors: Y
authors: Nukada
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.
authors: Kyo
authors: K
authors: Ei
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.
authors: Takayuki
authors: T
authors: Abo
authors: Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan.
authors: Hitoshi
authors: H
authors: Sakaguchi
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D002460:Cell Line; D003315:Cornea / Q000187:drug effects; D005128:Eye Diseases / Q000139:chemically induced*; D005188:False Negative Reactions; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity*; D011237:Predictive Value of Tests; D011817:Rabbits; D015203:Reproducibility of Results; D013501:Surface-Active Agents
chemical_list: D007509:Irritants; D013501:Surface-Active Agents
keywords: Alternative method; Chemical mixture; Eye irritation; SIRC cell line; STE