title: Yet another way that phage λ manipulates its Escherichia coli host: λrexB is involved in the lysogenic-lytic switch.
abstract: The life cycle of phage λ has been studied extensively. Of particular interest has been the process leading to the decision of the phage to switch from lysogenic to lytic cycle. The principal participant in this process is the λcI repressor, which is cleaved under conditions of DNA damage. Cleaved λcI no longer acts as a repressor, allowing phage λ to switch from its lysogenic to lytic cycle. The well-known mechanism responsible for λcI cleavage is the SOS response. We have recently reported that the Escherichia coli toxin-antitoxin mazEF pathway inhibits the SOS response; in fact, the SOS response is permitted only in E. coli strains deficient in the expression of the mazEF pathway. Moreover, in strains lysogenic for prophage λ, the SOS response is enabled by the presence of λrexB. λRexB had previously been found to inhibit the degradation of the antitoxin MazE, thereby preventing the toxic action of MazF. Thus, phage λ rexB gene not only safeguards the prophage state by preventing death of its E. coli host but is also indirectly involved in the lysogenic-lytic switch.
authors: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem, 91120, Israel.
authors: Hanna
authors: H
authors: Engelberg-Kulka
authors: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem, 91120, Israel.
authors: Sathish
authors: S
authors: Kumar
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D029968:Escherichia coli Proteins / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008242:Lysogeny; D039002:Prophages / Q000235:genetics / Q000502:physiology; D013014:SOS Response, Genetics; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C422955:MazF protein, E coli; D014764:Viral Proteins; C069331:rexB protein, Bacteriophage lambda; D004722:Endoribonucleases