title: Differential gene expression in Aspergillus fumigatus induced by human platelets in vitro.
abstract: Invasive aspergillosis is characterized by vascular invasion and thrombosis. In order to determine the antifungal activity of human platelets, hyphal elongation and metabolic activity of a clinical A. fumigatus isolate were measured. Genome-wide identification of differentially expressed genes in A. fumigatus was performed after exposure to platelets for 15, 30, 60 and 180 min. Data were analyzed by gene ontology annotation as well as functional categories (FunCat) and KEGG enrichment analyses. Platelets attenuated hyphal elongation and viability of A. fumigatus and in total 584 differentially expressed genes were identified, many of which were associated with regulation of biological processes, stress response, transport and metabolism. FunCat and KEGG enrichment analyses showed stress response and metabolic adaptation to be increased in response to platelets. Our findings demonstrate that A. fumigatus displayed a specific transcriptional response when exposed to platelets, thus reflecting their antifungal activities.
authors: University of Applied Sciences Tyrol, 6020 Innsbruck, Austria; Division of Hygiene and Medical Microbiology, Innsbruck Medical University, 6020 Innsbruck, Austria. Electronic address: susanne.perkhofer@fhg-tirol.ac.at.
authors: Susanne
authors: S
authors: Perkhofer
authors: University of Applied Sciences Tyrol, 6020 Innsbruck, Austria.
authors: Christoph
authors: C
authors: Zenzmaier
authors: Center for Infection and Immunity of Lille (CIIL), Institut Pasteur de Lille, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), INSERM U1019, CNRS UMR 8204, Univ. Lille Nord de France, Lille, France.
authors: Emilie
authors: E
authors: Frealle
authors: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors: Michael
authors: M
authors: Blatzer
authors: Division of Bioinformatics, Biocenter, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors: Hubert
authors: H
authors: Hackl
authors: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors: Bettina
authors: B
authors: Sartori
authors: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors: Cornelia
authors: C
authors: Lass-Flörl
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001792:Blood Platelets / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions; D006801:Humans; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D050296:Microbial Viability / Q000187:drug effects
chemical_list: D000935:Antifungal Agents
keywords: Aspergillosis; Aspergillus fumigatus; Microarray; Platelets; Thrombocytes; Transcriptome