title: The correlation between miRNA and lymph node metastasis in gastric cancer.
abstract: Lymph node metastasis (LNM) in gastric cancer is associated with higher rate of cancer recurrence and poor prognosis. As a result, a reliable biomarker for the prediction of LNM is important and would be valuable in the clinical practice. MiRNA microarray revealed that ten miRNAs were expressed significantly different among patients with or without LNM. A total of 46 gastric cancer patients were enrolled and divided into two groups (23 in each group) according to the presence or absence of LNM. RT-PCR of these 10 miRNAs was investigated and compared between the two groups. MiR-1207-5p was significantly upregulated in gastric cancer patients without LNM compared with those with LNM. Patients with upregulated miR-1207-5p had less scirrhous stromal reaction, less lymphovascular invasion, and earlier pathological T category, N category, and TNM stage, compared with those with downregulated or unchanged miR-1207-5p. Multivariate analysis showed that stromal reaction type, lymphovascular invasion, pathological T category and TNM stage, and expression of miR-1207-5p were independent risk factors of LNM. MiR-1207-5p could serve as a useful biomarker in the prediction of LNM in gastric cancer.
authors: Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei City 11217, Taiwan ; School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan.
authors: Kuo-Hung
authors: KH
authors: Huang
authors: School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; Division of Colon & Rectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei City 11217, Taiwan.
authors: Yuan-Tzu
authors: YT
authors: Lan
authors: Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei City 11217, Taiwan ; School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan.
authors: Wen-Liang
authors: 0000-0001-5726-1066
authors: WL
authors: Fang
authors: School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; En Chu Kong Hospital, New Taipei City 237, Taiwan.
authors: Jen-Hao
authors: JH
authors: Chen
authors: School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; National Yang-Ming University Hospital, Yilan City 260, Taiwan.
authors: Su-Shun
authors: SS
authors: Lo
authors: School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; Department of Pathology, Taipei Veterans General Hospital, Taipei City 11217, Taiwan.
authors: Anna Fen-Yau
authors: AF
authors: Li
authors: Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan ; Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei City 11217, Taiwan ; Institute of Pharmacology, National Yang-Ming University, Taipei City 11221, Taiwan.
authors: Shih-Hwa
authors: SH
authors: Chiou
authors: Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei City 11217, Taiwan ; School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan.
authors: Chew-Wun
authors: CW
authors: Wu
authors: Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei City 11217, Taiwan ; School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan.
authors: Yi-Ming
authors: YM
authors: Shyr
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011446:Prospective Studies; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs