title: CIA2 deficiency results in impaired oxidative stress response and enhanced intracellular basal UPR activity in Saccharomyces cerevisiae.
abstract: Yeast Cia2p is a component of the cytosolic Fe/S protein assembly (CIA) machinery. Initial studies of the CIA machinery were performed in yeast, but the precise role of Cia2p in this eukaryote is still unknown. We report that CIA2 deficiency results in impaired oxidative stress response, as evidenced by increased sensitivity to the oxidant cumene hydroperoxide (CHP), impaired activities of superoxide dismutases and aconitase and decreased replicative lifespan in the mutants. Moreover, intracellular reactive oxygen species levels were significantly increased in CIA2-deficient cells after treatment with CHP. We also show that CIA2-deficient cells display an increased resistance to tunicamycin-induced endoplasmic reticulum (ER) stress, as evidenced by the upregulated splicing of the mRNA of HAC1, which encodes a functional transcription factor that regulates the transcription of unfolded protein response (UPR) target genes, suggesting enhanced intracellular UPR activity. Furthermore, the transcription of several canonical UPR target genes is strongly induced in CIA2-deficient cells as compared with wild-type controls. Taken together, these results suggest the involvement of Cia2p in oxidative and ER stress responses in yeast.
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors: Wei
authors: W
authors: Zhao
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors: Hua-Zhen
authors: HZ
authors: Zheng
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China.
authors: Yu-Jie
authors: YJ
authors: Niu
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors: Yuan
authors: Y
authors: Yuan
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors: Bing-Xiong
authors: BX
authors: Fang
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China Second Clinical Medical School, Guangdong Medical College, Dongguan 523808, China.
authors: Yi-Na
authors: YN
authors: Liu
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China Second Clinical Medical School, Guangdong Medical College, Dongguan 523808, China.
authors: Lu-Hui
authors: LH
authors: Cai
authors: Department of Biochemistry, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong.
authors: Zhong-Jun
authors: ZJ
authors: Zhou
authors: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China gdmclxg@126.com.
authors: Xin-Guang
authors: XG
authors: Liu
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D001555:Benzene Derivatives / Q000494:pharmacology; D003593:Cytoplasm / Q000235:genetics / Q000502:physiology; D059865:Endoplasmic Reticulum Stress; D009154:Mutation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors; D014415:Tunicamycin / Q000494:pharmacology; D056811:Unfolded Protein Response / Q000235:genetics; D015854:Up-Regulation
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D001555:Benzene Derivatives; C091341:HAC1 protein, S cerevisiae; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C555077:YHR122W protein, S cerevisiae; D014415:Tunicamycin; C007164:cumene hydroperoxide
keywords: CIA2; UPR; oxidative stress