title: [Hematopoietic reconstitution after transplantation of uncontrolled-rate cryopreservation autologous peripheral blood hematopoietic stem cells using -80 °C mechanical freezer].
abstract: OBJECTIVE
  This study was to identify the efficacy of -80°C cryopreservated peripheral blood hemato-poietic stem cell (PBHSC) transplantation for hematopoietic reanstitution in patients.
  
  
  METHODS
  The efficacy of 104 patients underwent autologous peripheral blood hematopoietic stem cell transplantation using uncontrolled-rate freezing and storage at -80°C was evaluated.
  
  
  RESULTS
  This cryopreservation method could effectively cryopreserve peripheral blood stem cells. Out of 104 patients only 2 patients died, other patients got hematologic reconstition satisfactorily, the median engrafement times of neutrophils and platelet were 12 and 14 days respectively, the activity of cells after rehabilitation was 94%, the mean recovery rates of CD34(+) cells and mononuclear cells (MNC) were 86% and 80.3% respectively. There were no significant influences on engrafement time in sex, chemotherapy circles and radiotherapy. The engrafement of leukocytes associated with amount of CD34(+) cells.
  
  
  CONCLUSIONS
  This simple uncontrolled-rate freezing PBHSC at -80°C is safe, effective and economic, and can meet clinical needs. As compared with the classical cryopreservation, there were no significant differences in hematopoietic reconstitution. Therefore, this method worth to popularize and apply in clinic.
authors: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors: Mo
authors: M
authors: Liu
authors: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors: Yu
authors: Y
authors: Zhao
authors: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors: Jing-Fen
authors: JF
authors: Sun
authors: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors: Wei
authors: W
authors: Zhao
authors: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors: Li-Li
authors: LL
authors: Wang
authors: Department of Hematology, PLA General Hospital,Beijing 100853, China. E-mail: chunhuiliyu@yahoo.com.
authors: Li
authors: L
authors: Yu
mesh_terms: D001792:Blood Platelets; D001793:Blood Preservation; D015925:Cryopreservation; D005615:Freezing; D006412:Hematopoietic Stem Cells; D006801:Humans; D007962:Leukocytes; D009504:Neutrophils; D036102:Peripheral Blood Stem Cell Transplantation