title: Interleukin-10 but not transforming growth factor-β1 gene expression is up-regulated by vitamin D treatment in multiple sclerosis patients.
abstract: Multiple sclerosis (MS) is an inflammatory and autoimmune disease. Variety of different genetics and environmental factors are involved in MS pathology. The epidemiological studies demonstrated that vitamin D has immune and immunomodulating effects on MS disease. Therefore, this study aims to evaluate the effect of vitamin D treatment on the expression of interleukin-10 (IL-10) and transforming growth factor-β1 (TGF-β1) genes in MS patients. We found that, the expression level of IL-10 gene in treated patients was up-regulated 3.84 times more than before treatment, but the expression level of TGF-β1 was not affected by vitamin D treatment. Also, a significant relationship was observed between vitamin D level and EDSS in MS patients. Our results indicated that the increased level of serum vitamin D and IL-10 gene expression may be associated with the reduction of EDSS scores in MS patients.
authors: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Zeinab Shirvani
authors: ZS
authors: Farsani
authors: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: behmanesh@modares.ac.ir.
authors: Mehrdad
authors: M
authors: Behmanesh
authors: MS Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Sahraian
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood* / Q000188:drug therapy*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D016896:Treatment Outcome; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*; D014807:Vitamin D / Q000008:administration & dosage* / Q000097:blood; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D014807:Vitamin D
keywords: EDSS; IL-10; Multiple sclerosis; TGF-β1; Vitamin D; qRT-PCR