title: Curcumin mitigates the fibrillation of human serum albumin and diminishes the formation of reactive oxygen species.
abstract: The formation of amyloid fibrils are thought to contribute to pathogenesis of many amyloids associated human diseases. Here the impact of curcumin on amyloid formation of human serum albumin (HSA) was studied. Incubation of HSA at 68°C under physiologic pH led to amyloid fibril formation. Thioflavin T (ThT) fluorescence was used for determination of amyloid fibril formation. Atomic force microscopy experiments indicated different fibril structure of HSA incubated with or without curcumin. The monitoring of the changes in reactive oxygen species (ROS) levels upon incubation of curcumin with HSA showed a significant decrease in ROS levels. Similar experiments were also carried out in the presence of aflatoxin M1 (AFM1) and lead (Pb) ions. Our results indicated that AFM1 and Pb ions promote the fibrillation of HSA and accelerate ROS production, which were inhibited in the presence of curcumin. Thus, curcumin mitigates protein fibrillation activity and diminishes ROS generation.
authors: Mansooreh
authors: M
authors: Mazaheri
authors: Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran. moosavi@ut.ac.ir.
authors: Ali Akbar
authors: AA
authors: Moosavi-Movahedi
authors: Ali Akbar
authors: AA
authors: Saboury
authors: Mehran Habibi
authors: MH
authors: Rezaei
authors: Mostafa
authors: M
authors: Shourian
authors: Mohammad
authors: M
authors: Farhadi
authors: Nader
authors: N
authors: Sheibani
mesh_terms: D016607:Aflatoxin M1 / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007854:Lead / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D007854:Lead; D016607:Aflatoxin M1; D003474:Curcumin