title: miR-342-3p targets RAP2B to suppress proliferation and invasion of non-small cell lung cancer cells.
abstract: MicroRNAs (miRNAs) play critical roles in cancer development and progression. In this study, we examined the roles and molecular mechanisms of miR-342-3p in human non-small cell lung cancer (NSCLC). The results showed that miR-342-3p is downregulated in NSCLC cell lines and tissues, and its overexpression induces significant inhibition of NSCLC cell proliferation, invasion, and tumor growth in nude mice. In addition, miR-342-3p repressed RAP2B expression through interactions with its 3'-UTR region. Restoration of RAP2B expression reversed miR-342-3p-mediated inhibitory activity in NSCLC cells. Finally, analyses of miR-342-3p and RAP2B levels in NSCLCs revealed that miR-342-3p inversely correlated with RAP2B mRNA expression. Our collective findings provide preliminary evidence that miR-342-3p acts as a tumor suppressor in NSCLC through repression of RAP2B.
authors: Department of Cardio-thoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, 200092, China.
authors: Xiao
authors: X
authors: Xie
authors: Hongtao
authors: H
authors: Liu
authors: Mingsong
authors: M
authors: Wang
authors: Fangbao
authors: F
authors: Ding
authors: Haibo
authors: H
authors: Xiao
authors: Fengqing
authors: F
authors: Hu
authors: Rui
authors: R
authors: Hu
authors: Ju
authors: J
authors: Mei
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays; D020668:rap GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C546176:MIRN342 microRNA, human; D035683:MicroRNAs; C065562:RAP2B protein, human; D020668:rap GTP-Binding Proteins