title: Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future.
authors: College of Life Sciences, Henan Normal University, Xinxiang 453007, China; College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Xibao
authors: X
authors: Shi
authors: College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Yongzhe
authors: Y
authors: Chang
authors: College of Life Sciences, Henan Normal University, Xinxiang 453007, China; College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
authors: Xiaozhuan
authors: X
authors: Zhang
authors: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Li
authors: L
authors: Wang
authors: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors: Chunxi
authors: C
authors: Li
authors: College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
authors: Kai
authors: K
authors: Jiang
authors: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Jing
authors: J
authors: Chen
authors: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Chao
authors: C
authors: Wang
authors: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
authors: Ruiguang
authors: R
authors: Deng
authors: The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors: Jianming
authors: J
authors: Fan
authors: College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. Electronic address: zhanggaiping2003@163.com.
authors: Gaiping
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D000882:Haplorhini; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering; D017361:Viral Nonstructural Proteins
keywords: PRRSV; Small interfering RNA; nsp1α