title: Osmotic fragility of red blood cells, lipid peroxidation and Ca²⁺-ATPase activity of placental homogenates and red blood cell ghosts in salt-loaded pregnant rats.
abstract: OBJECTIVE
  To evaluate the osmotic fragility of red blood cells and the level of lipid peroxidation, the Ca(2+)-ATPase activity of red cell ghosts and placental homogenates from salt-loaded pregnant rats.
  
  
  METHODS
  Salt-loaded pregnant rats received 1.8% NaCl solution ad libitum as a beverage for seven days, starting on 15th day of pregnancy. Then, it was evaluated the level of lipid peroxidation and the Ca(2+)-ATPase activity of placental homogenates and red blood cell ghosts from control and experimental rats. Furthermore, the osmotic fragility of the red blood cells was evaluated by measuring the lysis of these cells when incubated with a NaCl solution with different osmolarities.
  
  
  RESULTS
  It was found that placental homogenates and red blood cell ghosts from experimental pregnant rats showed an increased level of lipid peroxidation and a lowered Ca(2+)-ATPase activity, as compared to control pregnant rats. They also presented an increased osmotic fragility of their red blood cells.
  
  
  CONCLUSIONS
  Salt-loaded pregnant rats showed, similar to preeclamptic women, an increased level of lipid peroxidation and a lowered Ca(2+)-ATPase activity in placental and red blood cells membranes, as well as an increased osmotic fragility of the red blood cells.
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Deliana
authors: D
authors: Rojas
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Freddy
authors: F
authors: Rodríguez
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Juvell
authors: J
authors: Barráez
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Sandy
authors: S
authors: Piñero
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Delia I
authors: DI
authors: Chiarello
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Cilia
authors: C
authors: Abad
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Reinaldo
authors: R
authors: Marín
authors: a Laboratorio de Bioenergética Celular , Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela.
authors: Fulgencio
authors: F
authors: Proverbio
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004195:Disease Models, Animal; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005260:Female; D015227:Lipid Peroxidation; D009996:Osmotic Fragility; D010920:Placenta / Q000201:enzymology*; D011225:Pre-Eclampsia / Q000097:blood / Q000201:enzymology*; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D012965:Sodium Chloride; D000252:Calcium-Transporting ATPases
keywords: Ca2+-ATPase; lipid peroxidation; osmotic fragility; preeclampsia