title: Encapsulation of hydrophobic allergens into nanoparticles improves the in vitro immunological diagnosis of allergic contact dermatitis.
abstract: The diagnosis of allergic contact dermatitis (ACD) relies on in vivo patch testing. In vitro immunological assays based on the characterization of circulating allergen-specific memory T cells represent a promising alternative to patch testing. However, their development is hampered by the technical challenge of assessing hydrophobic allergens in serum-based assays. In this study, we show that the encapsulation of fragrance mix 1 (FMI, a mixture of 8 hydrophobic allergens) into poly-ε-caprolactone nanoparticle (NP) vectors: (1) dramatically increases the solubilization of allergens in conventional cell culture media and (2) allows for a robust in vitro reactivation of allergen-specific T cells in large numbers of fragrance allergic patients. Therefore, the encapsulation of hydrophobic allergens into NP vectors opens new avenues to improve the in vitro immunobiological diagnosis of ACD.
  
  
  BACKGROUND
  Allergic Contact Dermatitis (ACD) is a delayed-type hypersensivity reaction prevalent in many individuals. Currently, skin patch testing has been the mainstay for diagnosis clinically. In this study, the authors described an improvement to in vitro immunological assays measuring circulating allergen-specific memory T cells, using nanoparticle vectors. The positive data might provide an exciting alternative to current practice of patch-testing.
authors: UMR CNRS 5007, Laboratoire d'Automatique et de Génie des Procédés, Université Lyon1, Lyon, France; CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France.
authors: Angèle
authors: A
authors: Cortial
authors: CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France; Allergology & Clinical Immunology, CH Lyon-Sud, Pierre-Bénite, France.
authors: Audrey
authors: A
authors: Nosbaum
authors: CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France.
authors: Aurore
authors: A
authors: Rozières
authors: Cliniques Universitaires Saint-Luc, Belgique, Université Catholique de Louvain, Brussels, Belgium.
authors: Marie
authors: M
authors: Baeck
authors: Cliniques Universitaires Saint-Luc, Belgique, Université Catholique de Louvain, Brussels, Belgium.
authors: Laurence
authors: L
authors: de Montjoye
authors: Allergology & Clinical Immunology, CH Lyon-Sud, Pierre-Bénite, France.
authors: Sophie
authors: S
authors: Grande
authors: UMR CNRS 5007, Laboratoire d'Automatique et de Génie des Procédés, Université Lyon1, Lyon, France.
authors: Stéphanie
authors: S
authors: Briançon
authors: CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France; Allergology & Clinical Immunology, CH Lyon-Sud, Pierre-Bénite, France.
authors: Jean-François
authors: JF
authors: Nicolas
authors: CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France. Electronic address: marc.vocanson@inserm.fr.
authors: Marc
authors: M
authors: Vocanson
mesh_terms: D000368:Aged; D000485:Allergens; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008875:Middle Aged; D053758:Nanoparticles; D011091:Polyesters
chemical_list: D000485:Allergens; D011091:Polyesters; C016240:polycaprolactone
keywords: Allergic contact dermatitis; Diagnosis; Fragrances; Immunobiological assays; Poly-ε-caprolactone nanoparticles