title: A proteomics survey on wheat susceptibility toFusariumhead blight during grain development.
abstract: The mycotoxigenic fungal speciesFusarium graminearumis able to attack several important cereal crops, such as wheat and barley. By causingFusariumHead Blight (FHB) disease,F. graminearuminduces yield and quality losses and poses a public health concern due toin plantamycotoxin production. The molecular and physiological plant responses to FHB, and the cellular biochemical pathways used byF. graminearumto complete its infectious process remain still unknown. In this study, a proteomics approach, combining 2D-gel approach and mass spectrometry, has been used to determine the specific protein patterns associated with the development of the fungal infection during grain growth on susceptible wheat. Our results reveal thatF. graminearuminfection does not deeply alter the grain proteome and does not significantly disturb the first steps of grain ontogeny but impacts molecular changes during the grain filling stage (impact on starch synthesis and storage proteins). The differentially regulated proteins identified were mainly involved in stress and defence mechanisms, primary metabolism, and main cellular processes such as signalling and transport. Our survey suggests thatF. graminearumcould take advantage of putative susceptibility factors closely related to grain development processes and thus provide new insights into key molecular events controlling the susceptible response to FHB in wheat grains.
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Cherif
authors: C
authors: Chetouhi
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Ludovic
authors: L
authors: Bonhomme
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Philippe
authors: P
authors: Lecomte
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Florence
authors: F
authors: Cambon
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Marielle
authors: M
authors: Merlino
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
authors: David Georges
authors: DG
authors: Biron
authors: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France; UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F-63100 Clermont-Ferrand, France.
authors: Thierry
authors: T
authors: Langin
keywords: FHB; Fusarium graminearum; Mycotoxin; Susceptibility factors; Wheat