title: Isotope sensitive branching and kinetic isotope effects to analyse multiproduct terpenoid synthases from Zea mays.
abstract: Multiproduct terpene synthases TPS4-B73 and TPS5-Delprim from Zea mays exhibit isotopically sensitive branching in the formation of mono- and sesquiterpene volatiles. The impact of the kinetic isotope effects and the stabilization of the reactive intermediates by hyperconjugation along with the shift of products from alkenes to alcohols are discussed.
authors: Max Planck Institute for Chemical Ecology, Hans-Knöll-Strasse 8, D-07745 Jena, Germany. boland@ice.mpg.deb.
authors: Nathalie
authors: N
authors: Gatto
authors: Abith
authors: A
authors: Vattekkatte
authors: Tobias
authors: T
authors: Köllner
authors: Jörg
authors: J
authors: Degenhardt
authors: Jonathan
authors: J
authors: Gershenzon
authors: Wilhelm
authors: W
authors: Boland
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry*; D011756:Diphosphates / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry*; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry*; D011106:Polyisoprenyl Phosphates / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry*; D003313:Zea mays / Q000201:enzymology*
chemical_list: D011756:Diphosphates; D004224:Diterpenes; D010940:Plant Proteins; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C511282:geranyl diphosphate; C004808:farnesyl pyrophosphate; D003903:Deuterium; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase