title: Biomimetic hybrid nanofibrous substrates for mesenchymal stem cells differentiation into osteogenic cells.
abstract: Mimicking native extracellular matrix with electrospun porous bio-composite nanofibrous scaffolds has huge potential in bone tissue regeneration. The aim of this study is to fabricate porous poly(l-lactic acid)-co-poly-(ε-caprolactone)/silk fibroin/ascorbic acid/tetracycline hydrochloride (PLACL/SF/AA/TC) and nanohydroxyapatite (n-HA) was deposited by calcium-phosphate dipping method for bone tissue engineering (BTE). Fabricated nanofibrous scaffolds were characterized for fiber morphology, hydrophilicity, porosity, mechanical test and chemical properties by FT-IR and EDX analysis. The results showed that the fiber diameter and pore size of scaffolds observed around 228±62-320±22nm and 1.5-6.9μm respectively. Resulting nanofibrous scaffolds are highly porous (87-94%) with ultimate tensile strength observed in the range of 1.51-4.86MPa and also showed better hydrophilic properties after addition of AA, TC and n-HA. Human mesenchymal stem cells (MSCs) cultured on these bio-composite nanofibrous scaffolds and stimulated to osteogenic differentiation in the presence of AA/TC/n-HA for BTE. The cell proliferation and biomaterial interactions were studied using MTS assay, SEM and CMFDA dye exclusion methods. Osteogenic differentiation of MSCs was proven by using alkaline phosphatase activity, mineralization and double immunofluorescence staining of both CD90 and osteocalcin. The observed results suggested that the fabricated PLACL/SF/AA/TC/n-HA biocomposite hybrid nanofibrous scaffolds have good potential for the differentiation of MSCs into osteogenesis for bone tissue engineering.
authors: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors: Chinnasamy
authors: C
authors: Gandhimathi
authors: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
authors: Jayarama Reddy
authors: JR
authors: Venugopal
authors: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors: Allister Yingwei
authors: AY
authors: Tham
authors: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
authors: Seeram
authors: S
authors: Ramakrishna
authors: Cellular and Molecular Epigenetics Lab, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore. Electronic address: dineshkumar@ntu.edu.sg.
authors: Srinivasan Dinesh
authors: SD
authors: Kumar
mesh_terms: D000469:Alkaline Phosphatase / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology; D040761:Biomimetic Materials / Q000494:pharmacology*; D032701:Biomimetics / Q000379:methods; D002130:Calcium Phosphates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology; D005109:Extracellular Matrix / Q000187:drug effects; D005349:Fibroins / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology; D016062:Porosity; D012038:Regeneration / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; C482288:fibroin, silkworm; C550605:poly(lactic acid-co-epsilon-caprolactone); D005349:Fibroins; D017886:Durapatite; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D013752:Tetracycline; D001205:Ascorbic Acid
keywords: Mineralization; Nanofibrous scaffolds; Nanohydroxyapatite; PLACL; Silk fibroin