title: Rapid preparation of (methyl)malonyl coenzyme A and enzymatic formation of unusual polyketides by type III polyketide synthase from Aquilaria sinensis.
abstract: (Methyl)malonyl coenzyme A was rapidly and effectively synthesized by a two-step procedure involving preparation of N-hydroxysuccinimidyl (methyl)malonate from (methyl)Meldrum's acid, and followed by transesterification with coenzyme A. The synthesized (methyl)malonyl coenzyme A could be well accepted and assembled to 4-hydroxy phenylpropionyl coenzyme A by type III polyketide synthase from Aquilaria sinensis to produce dihydrochalcone and 4-hydroxy-3,5-dimethyl-6-(4-hydroxyphenethyl)-2H-pyrone as well as 4-hydroxy-3,5-dimethyl-6-(5-(4-hydroxyphenyl)-3-oxopentan-2-yl)-2H-pyrone.
authors: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Baotou Medical College, Baotou 014060, China.
authors: Bo-Wen
authors: BW
authors: Gao
authors: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Xiao-Hui
authors: XH
authors: Wang
authors: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors: Xiao
authors: X
authors: Liu
authors: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: shishepo@163.com.
authors: She-Po
authors: SP
authors: Shi
authors: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: pengfeitu@163.com.
authors: Peng-Fei
authors: PF
authors: Tu
mesh_terms: D000217:Acyltransferases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D047188:Chalcones / Q000378:metabolism; D004146:Dioxanes / Q000737:chemistry / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D010802:Phylogeny; D061065:Polyketides / Q000378:metabolism*; D011753:Pyrones / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D029645:Thymelaeaceae / Q000201:enzymology*
chemical_list: D047188:Chalcones; D004146:Dioxanes; D061065:Polyketides; D011753:Pyrones; D011994:Recombinant Proteins; D008316:Malonyl Coenzyme A; C076489:Meldrum's acid; D000217:Acyltransferases; C020141:flavanone synthetase; C015812:dihydrochalcone
keywords: Aquilaria sinensis; Malonyl-CoA; Meldrum’s acid; Methylmalonyl-CoA; Type III polyketide synthase