title: Comparative sequence analysis of double stranded RNA binding protein encoding gene of parapoxviruses from Indian camels.
abstract: The dsRNA binding protein (RBP) encoding gene of parapoxviruses (PPVs) from the Dromedary camels, inhabitating different geographical region of Rajasthan, India were amplified by polymerase chain reaction using the primers of pseudocowpoxvirus (PCPV) from Finnish reindeer and cloned into pGEM-T for sequence analysis. Analysis of RBP encoding gene revealed that PPV DNA from Bikaner shared 98.3% and 76.6% sequence identity at the amino acid level, with Pali and Udaipur PPV DNA, respectively. Reference strains of Bovine papular stomatitis virus (BPSV) and PCPV (reindeer PCPV and human PCPV) shared 52.8% and 86.9% amino acid identity with RBP gene of camel PPVs from Bikaner, respectively. But different strains of orf virus (ORFV) from different geographical areas of the world shared 69.5-71.7% amino acid identity with RBP gene of camel PPVs from Bikaner. These findings indicate that the camel PPVs described are closely related to bovine PPV (PCPV) in comparison to caprine and ovine PPV (ORFV).
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: G
authors: G
authors: Nagarajan
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: Shelesh Kumar
authors: SK
authors: Swami
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: Shyam Singh
authors: SS
authors: Dahiya
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: G
authors: G
authors: Sivakumar
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: F C
authors: FC
authors: Tuteja
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: S D
authors: SD
authors: Narnaware
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: S C
authors: SC
authors: Mehta
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: Raghvendar
authors: R
authors: Singh
authors: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
authors: N V
authors: NV
authors: Patil
keywords: Camel; India; Parapoxvirus; dsRNA binding protein encoding gene