title: Identification of transcription factor AML-1 binding site upstream of human cytomegalovirus UL111A gene.
abstract: Human cytomegalovirus (HCMV) interleukin-10 (hcmvIL-10), encoded by HCMV UL111A gene, is a homolog of human IL-10. It exerts immunomodulatory effects that allow HCMV to evade host defense mechanisms. However, the exact mechanism underlying the regulation of hcmvIL-10 expression is not well understood. The transcription factor acute myeloid leukemia 1 (AML-1) plays an important role in the regulation of various genes involved in the differentiation of hematopoietic lineages. A putative AML-1 binding site is present within the upstream regulatory region (URR) of UL111A gene. To provide evidence that AML-1 is involved in regulating UL111A gene expression, we examined the interaction of AML-1 with the URR of UL111A in HCMV-infected human monocytic THP-1 cells using a chromatin immunoprecipitation assay. HcmvIL-10 transcription was detected in differentiated THP-1 cells, but not in undifferentiated ones. Furthermore, the URR of UL111A showed a higher intensity of AML-1 binding, a higher level of histone H3 acetyl-K9, but a lower level of histone H3 dimethyl-K9 in differentiated THP-1 cells than undifferentiated cells. Down-regulation of AML1 by RNA interference decreased the expression of the UL111A gene. Our results suggest that AML-1 may contribute to the epigenetic regulation of UL111A gene via histone modification in HCMV-infected differentiated THP-1 cells. This finding could be useful for the development of new anti-viral therapies.
authors: Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, China; Department of Laboratory Medicine, the Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; School of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China; Key Laboratory of Laboratory Medicine, Education Ministry of China, Wenzhou, Zhejiang, China.
authors: Xiaoqun
authors: X
authors: Zheng
authors: The First People's Hospital of Changde, Changde, Hunan, China.
authors: Yan
authors: Y
authors: Gao
authors: Department of Laboratory Medicine, the Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; School of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China; Key Laboratory of Laboratory Medicine, Education Ministry of China, Wenzhou, Zhejiang, China.
authors: Qi
authors: Q
authors: Zhang
authors: Department of Laboratory Medicine, the Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; School of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China; Key Laboratory of Laboratory Medicine, Education Ministry of China, Wenzhou, Zhejiang, China.
authors: Yanqing
authors: Y
authors: Liu
authors: School of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China; Key Laboratory of Laboratory Medicine, Education Ministry of China, Wenzhou, Zhejiang, China.
authors: Ying
authors: Y
authors: Peng
authors: Jinhua Municipal Central Hospital, Jinhua, Zhejiang, China.
authors: Miao
authors: M
authors: Fu
authors: Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Yanhong
authors: Y
authors: Ji
mesh_terms: D001665:Binding Sites; D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics*; D015536:Down-Regulation; D044127:Epigenesis, Genetic; D015967:Gene Expression Regulation, Viral; D006657:Histones / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D006657:Histones; C493728:RUNX1 protein, human