title: miR-603 promotes glioma cell growth via Wnt/β-catenin pathway by inhibiting WIF1 and CTNNBIP1.
abstract: Gliomas are the most common and deadly type of brain tumor. In spite of progressive treatments, patient prognosis has not improved significantly. MicroRNAs are considered promising candidates for glioma therapy. MiR-603 was found overexpressed in both glioma tissues and cell lines. MiR-603 promoted cell proliferation, cell cycle progression and neurosphere formation. Conversely, inhibition of miR-603 remarkably reduced these effects. We confirmed that WIF1 and CTNNBIP1 are bona fide targets of miR-603. The negative correlation between miR-603 and these molecules' expression was shown by Pearson correlation in 50 primary glioma tissue samples. Furthermore, overexpression of miR-603 promoted nuclear β-catenin levels and TOPflash luciferase activity, indicating that miR-603 activates the Wnt/β-catenin signaling pathway. Our in vivo results confirmed the positive role of miR-603 in glioma development. We demonstrate that miR-603 regulates glioma development via its WIF1 and CTNNBIP1 targets, which suggests that miR-603 may be a promising candidate for therapeutic applications in glioma treatment.
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Mian
authors: M
authors: Guo
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Xiaoming
authors: X
authors: Zhang
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China; Department of Medical Service Management, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Guangzhi
authors: G
authors: Wang
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Jiahang
authors: J
authors: Sun
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors: Zhenfeng
authors: Z
authors: Jiang
authors: Dental School, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors: Kevork
authors: K
authors: Khadarian
authors: Department of Pathology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Shan
authors: S
authors: Yu
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Yan
authors: Y
authors: Zhao
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors: Chuncheng
authors: C
authors: Xie
authors: Department of Biological Chemistry, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.
authors: Kelvin
authors: K
authors: Zhang
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors: Minwei
authors: M
authors: Zhu
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors: Hong
authors: H
authors: Shen
authors: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors: Zhiguo
authors: Z
authors: Lin
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors: Chuanlu
authors: C
authors: Jiang
authors: Dental School, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: shenj02@gmail.com.
authors: Jia
authors: J
authors: Shen
authors: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China. Electronic address: zhengyongrihyd@163.com.
authors: Yongri
authors: Y
authors: Zheng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C495270:CTNNB1 protein, human; C411982:CTNNBIP1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C000597759:MIRN603 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C485569:WIF1 protein, human; D051176:beta Catenin
keywords: CTNNBIP1; Glioma; WIF1; Wnt/β-catenin; miR-603