title: Interaction of triblock co-polymer micelles with phospholipid-bilayer: a spectroscopic investigation using a potential chloride channel blocker.
abstract: Interaction of a potential chloride channel blocker, 9-methyl anthroate (9-MA), has been studied with zwitterionic l-α-phosphatidylcholine (egg-PC) lipid vesicles, which ascertains the utility of the drug as an efficient molecular reporter for probing the microheterogeneous environment of lipid-bilayers. The effect of a non-ionic triblock co-polymer P123 on the stability of these drug-bound lipid-bilayers has also been investigated by means of steady state and time-resolved spectroscopic techniques exploiting the fluorescence properties of the drug. Experimental results reveal that the addition of P123 to the drug-bound lipid results in a preferential complexation of the drug with the Pluronic leaving the lipid vesicles aside, which has been attributed to a substantially stronger binding interaction of the drug with P123 than that with egg-PC. The result is of potential interest from a medical perspective owing to the context of excess drug desorption from bio-membranes.
authors: Department of Chemistry, University of Calcutta, 92 A. P. C. Road, Calcutta-700009, India. nguchhait@yahoo.com.
authors: Aniruddha
authors: A
authors: Ganguly
authors: Soumen
authors: S
authors: Ghosh
authors: Nikhil
authors: N
authors: Guchhait
mesh_terms: D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D008823:Micelles; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018118:Chloride Channels; D008823:Micelles; D010743:Phospholipids; D011108:Polymers