title: Adsorption of human serum albumin onto highly orientated pyrolytic graphite surface studied by atomic force microscopy.
abstract: It is important to know the adsorption behavior and assembly structure of human serum albumin (HSA) molecules onto a carbonaceous substrate for further application of carbon nanomaterials in biomedical field. Individual HSA molecules and oligmers (dimer and trimer) adsorbed onto HOPG surface have been imaged by atomic force microscopy (AFM). Individual HSA molecule appeared as an ellipsoid on HOPG surface with average length of 12.6, width of 6.5, and height of 1.9 nm when they were incubated at the physiological condition (pH 7.4). HSA molecules also can form the interconnected chains, uniform network, and monolayer by tuning the initial concentrations and adsorption time. Furthermore, HSA molecules can assemble into quite different network structures and irregular chains at pH of 2, 5, and 10. This study could expand our knowledge of the interactions between protein and carbonaceous surfaces.
authors: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
authors: Xiao
authors: X
authors: Peng
authors: Haoran
authors: H
authors: Fu
authors: Ruisi
authors: R
authors: Liu
authors: Lin
authors: L
authors: Zhao
authors: Yuangang
authors: Y
authors: Zu
authors: Fengjie
authors: F
authors: Xu
authors: Zhiguo
authors: Z
authors: Liu
mesh_terms: D000327:Adsorption; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D055503:Protein Multimerization; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D012709:Serum Albumin; D006108:Graphite
keywords: HOPG; atomic force microscopy (AFM); human serum albumin (HSA)