title: Methods of glioma sample processing for molecular diagnosis for the glioma tissue bank project.
abstract: Genome-wide sequencing in glioma samples provides comprehensive insights into oncogenesis and malignant transformation. Several distinct biomarkers have been proven to have clinical significance and are being widely applied in routine clinical practice. Standard sample processing lays the foundation for successful molecular testing. In this study, we found intraoperative neuronavigation ensured higher tumor purity during sample collection, and an automated device helped improve DNA quality and increased yields. These two technologies are beneficial for glioma tissue bank construction and provide for accurate molecular testing during routine clinical practice.
authors: 1 Glioma Surgery Division, Neurological Surgery Department, Huashan Hospital, Shanghai Medical College, Fudan University , Shanghai, China .
authors: Zhi-feng
authors: ZF
authors: Shi
authors: Abudumijiti
authors: A
authors: Aibaidula
authors: Qi-sheng
authors: QS
authors: Tang
authors: Yi-wen
authors: YW
authors: Shen
authors: Hong
authors: H
authors: Chen
authors: Jin-song
authors: JS
authors: Wu
authors: Zhi-yong
authors: ZY
authors: Qin
authors: Jian-hong
authors: JH
authors: Zhu
authors: Ying
authors: Y
authors: Mao
authors: Liang-fu
authors: LF
authors: Zhou
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis; D005910:Glioma / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D006801:Humans; D038361:Neuronavigation / Q000379:methods*; D011786:Quality Control; D017422:Sequence Analysis, DNA / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D014015:Tissue Banks
chemical_list: D004273:DNA, Neoplasm