title: ABCE1 is a highly conserved RNA silencing suppressor.
abstract: ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosome-recycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia; Competence Centre for Cancer Research, Tallinn, Estonia.
authors: Kairi
authors: K
authors: Kärblane
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia; Competence Centre for Cancer Research, Tallinn, Estonia.
authors: Jelena
authors: J
authors: Gerassimenko
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Lenne
authors: L
authors: Nigul
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Alla
authors: A
authors: Piirsoo
authors: School of Life Sciences, Södertörn University College, S-14189, Huddinge, Sweden; Department of Biosciences and Nutrition, Karolinska Institute, S-14183, Huddinge, Sweden.
authors: Agata
authors: A
authors: Smialowska
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
authors: Kadri
authors: K
authors: Vinkel
authors: School of Life Sciences, Södertörn University College, S-14189, Huddinge, Sweden.
authors: Per
authors: P
authors: Kylsten
authors: School of Life Sciences, Södertörn University College, S-14189, Huddinge, Sweden; Department of Biosciences and Nutrition, Karolinska Institute, S-14183, Huddinge, Sweden.
authors: Karl
authors: K
authors: Ekwall
authors: Department of Biosciences and Nutrition, Karolinska Institute, S-14183, Huddinge, Sweden.
authors: Peter
authors: P
authors: Swoboda
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia; Competence Centre for Cancer Research, Tallinn, Estonia.
authors: Erkki
authors: E
authors: Truve
authors: Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia; Competence Centre for Cancer Research, Tallinn, Estonia.
authors: Cecilia
authors: C
authors: Sarmiento
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D010443:Peptide Chain Termination, Translational / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; D029742:Caenorhabditis elegans Proteins; D010940:Plant Proteins