title: Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
abstract: Recurrent airway obstruction (RAO) is an allergic disease of horses similar to human asthma, which is characterized by airway inflammation and activation of neutrophils, lymphocytes and platelets. Platelet activation and an increase in circulating platelet-leukocyte aggregates may lead to airway remodeling. The aim of this study was to investigate platelet status in RAO-affected horses based on the platelet morphology and platelet surface expression of CD41/61 and CD62P. Ten RAO-affected horses and ten healthy horses were included in this study. Blood samples were obtained to determine the platelet count (PLT), mean platelet volume (MPV) and platelet large cell ratio (P-LCR). Expression of CD62P and CD41/61 was detected by flow cytometry on activated platelets. The median PLT was significantly reduced in horses with RAO compared to the controls. The MPV and the P-LCR values were significantly higher in RAO horses than controls. Expression of CD41/61 on platelets was increased in RAO horses, while CD62P expression was reduced. This study demonstrated the morphological changes in platelets and expression of platelet surface receptors. Despite the decrease of CD62P expression, the observed increased surface expression of CD41/61 on platelets in horses with RAO may contribute to the formation of platelet aggregates in their respiratory system.
authors: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland. Electronic address: alicja.iwaszko@up.wroc.pl.
authors: Alicja
authors: A
authors: Iwaszko-Simonik
authors: Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-375 Wroclaw, Poland.
authors: Artur
authors: A
authors: Niedzwiedz
authors: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland.
authors: Stanislaw
authors: S
authors: Graczyk
authors: Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-375 Wroclaw, Poland.
authors: Malwina
authors: M
authors: Slowikowska
authors: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland.
authors: Aleksandra
authors: A
authors: Pliszczak-Krol
mesh_terms: D000402:Airway Obstruction / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D006734:Horse Diseases / Q000097:blood* / Q000276:immunology*; D006736:Horses; D006801:Humans; D039661:Integrin beta3 / Q000097:blood*; D008297:Male; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation; D040201:Platelet Membrane Glycoprotein IIb / Q000097:blood*; D012008:Recurrence
chemical_list: D039661:Integrin beta3; D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb
keywords: CD41/61; CD62P; Horses; Platelets; RAO; Recurrent airway obstruction