title: Alteration in abundance of specific membrane proteins of Aggregatibacter actinomycetemcomitans is attributed to deletion of the inner membrane protein MorC.
abstract: Aggregatibacter actinomycetemcomitans is an important pathogen in the etiology of human periodontal and systemic diseases. Inactivation of the gene coding for the inner membrane protein, morphogenesis protein C (MorC), results in pleotropic effects pertaining to the membrane structure and function of this bacterium. The role of this protein in membrane biogenesis is unknown. To begin to understand the role of this conserved protein, stable isotope dimethyl labeling in conjunction with MS was used to quantitatively analyze differences in the membrane proteomes of the isogenic mutant and wild-type strain. A total of 613 proteins were quantified and 601 of these proteins were found to be equal in abundance between the two strains. The remaining 12 proteins were found in lesser (10) or greater (2) abundance in the membrane preparation of the mutant strain compared with the wild-type strain. The 12 proteins were ascribed functions associated with protein quality control systems, oxidative stress responses, and protein secretion. The potential relationship between these proteins and the phenotypes of the MorC mutant strain is discussed.
authors: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, USA.
authors: Kenneth P
authors: KP
authors: Smith
authors: Department of Biology, University of Vermont, Burlington, VT, USA.|Vermont Genetics Network Proteomics Facility, University of Vermont, Burlington, VT, USA.
authors: Julia G
authors: JG
authors: Fields
authors: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, USA.
authors: Richard D
authors: RD
authors: Voogt
authors: Department of Biology, University of Vermont, Burlington, VT, USA.|Vermont Genetics Network Proteomics Facility, University of Vermont, Burlington, VT, USA.
authors: Bin
authors: B
authors: Deng
authors: Department of Biology, University of Vermont, Burlington, VT, USA.|Vermont Genetics Network Proteomics Facility, University of Vermont, Burlington, VT, USA.
authors: Ying-Wai
authors: YW
authors: Lam
authors: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, USA.
authors: Keith P
authors: KP
authors: Mintz
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D040901:Proteomics / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins
keywords: Chaperone proteins; Leukotoxin secretion; Microbiology; Type I secretion