title: Isolation and characterization of 20 polymorphic microsatellite loci in the migratory freshwater fish Leporinus obtusidens (Characiformes: Anostomidae) using 454 shotgun pyrosequencing.
abstract: Twenty polymorphic microsatellite markers were developed for the Neotropical fish Leporinus obtusidens using a next generation sequencing approach and tested in two other characifomes species, Schizodon platae and Prochilodus lineatus. Microsatellite loci alleles in L. obtusidens ranged between 2 and 20 alleles per locus (mean = 5·7), with expected heterozygosity values ranging from 0·097 to 0·956 (mean = 0·578) and observed heterozygosity values ranging from 0·000 to 0·800 (mean = 0·400) in a sample of 20 specimens from the lower Paraná River (Argentina). Most of these markers will be a valuable tool for captive breeding and stocking programmes, as well as for analyses of population connectivity and genetic structure in this broadly distributed Neotropical migratory fish.
authors: Instituto de Biología Molecular y Celular de Rosario (IBR) - CONICET, Universidad Nacional de Rosario, Ocampo y Esmeralda sede CCT, 2000 Rosario, Argentina; Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.
authors: G V
authors: GV
authors: Villanova
authors: M
authors: M
authors: Vera
authors: J
authors: J
authors: Díaz
authors: P
authors: P
authors: Martinez
authors: N B
authors: NB
authors: Calcaterra
authors: S E
authors: SE
authors: Arranz
mesh_terms: D000483:Alleles; D000818:Animals; D001118:Argentina; D060125:Characiformes / Q000235:genetics*; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D045483:Rivers
keywords: Neotropical fish; Paraná River; Prochilodus lineatus; Schizodon platae; boga; molecular marker