title: Up-regulation of miR-592 correlates with tumor progression and poor prognosis in patients with colorectal cancer.
abstract: miR-592, as a potential biomarker, has been linked to several cancers. However, the expression level and prognostic value of miR-592 in CRC have not been elucidated. In this study, we detected the miR-592 expression in CRC serum, tumor tissues, adjacent non-tumor tissues (NATs) and four colorectal cancer cell lines by RT-PCR. Our data proved that miR-592 expression was up-regulated in clinical CRC serum and tissues (P<0.05). Serum or tissue miR-592 in CRC metastatic patients also maintained a high level, compared to that in non-metastatic CRC patients (P<0.05). After radical surgery, postoperative serum miR-592 level in CRC patients significantly decreased (P<0.05). Our clinicopathological analysis revealed that high miR-592 was significant associated with the tumor size (P=0.008), TNM stage (P=0.026), distant metastasis (P=0.004) and preoperative CEA level (P=0.022), which led to a shorter overall survival rate in CRC patients (P=0.032). Furthermore, we designed and transfected miR-592 mimics or inhibitors into the corresponding CRC lines, and our experiments in vitro demonstrated that miR-592 could promote cell proliferation, wound healing and invasion ability of CRC cells (P<0.05), while miR-592 did not influence the CRC cell apoptosis (P>0.05). All these results suggested that miR-592 functioned as a novel and potential carcinogen-initiated and metastasis-related biomarker in CRC, and down-regulation of miR-592 might be considered as a potentially significant molecular treatment strategy for CRC patients.
authors: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Medical College, Xian Jiaotong University, Xian 710061, China.
authors: Mulin
authors: M
authors: Liu
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Qiaoming
authors: Q
authors: Zhi
authors: Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230000, China.
authors: Wenbin
authors: W
authors: Wang
authors: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, China.
authors: Qiao
authors: Q
authors: Zhang
authors: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, China.
authors: Taotao
authors: T
authors: Fang
authors: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Medical College, Xian Jiaotong University, Xian 710061, China. Electronic address: maqy_xjdfy@163.com.
authors: Qingyong
authors: Q
authors: Ma
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D013194:Staining and Labeling; D016019:Survival Analysis; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation / Q000235:genetics*; D014945:Wound Healing
chemical_list: C000588991:MIRN592 microRNA, human; D035683:MicroRNAs
keywords: Colorectal cancer; Prognosis; Progression; miR-592