title: Imaging approaches to measuring lysosomal calcium.
abstract: Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the cytosol upon activation of endolysosomal Ca(2+) channels. This local Ca(2+) signal is crucial for endolysosomal function and/or global Ca(2+) signaling. Importantly, defects in endolysosomal Ca(2+) are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca(2+), with particular emphasis on the inherent pitfalls that can plague the unwary.
authors: Department of Pharmacology, University of Oxford, Oxford, UK.
authors: Anthony J
authors: AJ
authors: Morgan
authors: Department of Pharmacology, University of Oxford, Oxford, UK.
authors: Lianne C
authors: LC
authors: Davis
authors: Department of Pharmacology, University of Oxford, Oxford, UK.
authors: Antony
authors: A
authors: Galione
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D011992:Endosomes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium
keywords: Bafilomycin A1; Ca(2+); GPN; Lysosome; NAADP; Nigericin; pH