title: Wild carrot oil extract is selectively cytotoxic to human acute myeloid leukemia cells.
abstract: In this study, we used Daucus carota oil extract (DCOE) to target acute myeloid leukemia (AML) cells. All the AML cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. Analysis of mechanism of cell death showed an increase in cells positive for annexinV and for active caspases, indicating that DCOE induces apoptotic cell death in AML. Inhibition of the MAPK pathway decreased sensitivity of AML cells to DCOE, indicating that cytotoxicity may be dependent on its activity. In conclusion, DCOE induces selective apoptosis in AML cells, possibly through a MAPK-dependent mechanism.
authors: Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, Beirut, Lebanon E-mail : ralph.abihabib@lau.edu.lb.
authors: Mirna
authors: M
authors: Tawil
authors: Amira
authors: A
authors: Bekdash
authors: Mohammad
authors: M
authors: Mroueh
authors: Costantine F
authors: CF
authors: Daher
authors: Ralph J
authors: RJ
authors: Abi-Habib
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D018552:Daucus carota / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D010938:Plant Oils