title: Similar serotonin-2A receptor binding in rats with different coping styles or levels of aggression.
abstract: Individual differences in coping style emerge as a function of underlying variability in the activation of a mesocorticolimbic brain circuitry. Particularly serotonin seems to play an important role. For this reason, we assessed serotonin-2A receptor (5-HT2A R) binding in the brain of rats with different coping styles. We compared proactive and reactive males of two rat strains, Wild-type Groningen (WTG) and Roman high- and low avoidance (RHA, RLA). 5-HT2A R binding in (pre)frontal cortex (FC) and hippocampus was investigated using a radiolabeled antagonist ([(3) H]MDL-100907) and agonist ([(3) H]Cimbi-36) in binding assays. No differences in 5-HT2A R binding were observed in male animals with different coping styles. [(3) H]MDL-100907 displayed a higher specific-to-nonspecific binding ratio than [(3) H]Cimbi-36. Our findings suggest that in these particular rat strains, 5-HT2A R binding is not an important molecular marker for coping style. Because neither an antagonist nor an agonist tracer showed any binding differences, it is unlikely that the affinity state of the 5-HT2A R is co-varying with levels of aggression or active avoidance in WTG, RHA and RLA.
authors: Department of Nuclear Medicine and Molecular Imaging, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
authors: Anniek K D
authors: AK
authors: Visser
authors: Anders
authors: A
authors: Ettrup
authors: Anders B
authors: AB
authors: Klein
authors: Aren
authors: A
authors: van Waarde
authors: Fokko J
authors: FJ
authors: Bosker
authors: Peter
authors: P
authors: Meerlo
authors: Gitte M
authors: GM
authors: Knudsen
authors: Sietse F
authors: SF
authors: de Boer
mesh_terms: D000223:Adaptation, Psychological / Q000187:drug effects*; D000374:Aggression / Q000502:physiology*; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D001596:Benzylamines / Q000493:pharmacokinetics; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005464:Fluorobenzenes / Q000493:pharmacokinetics; D008297:Male; D010627:Phenethylamines / Q000493:pharmacokinetics; D010880:Piperidines / Q000493:pharmacokinetics; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D012702:Serotonin Antagonists / Q000493:pharmacokinetics; D017366:Serotonin Receptor Agonists / Q000493:pharmacokinetics; D014316:Tritium / Q000493:pharmacokinetics
chemical_list: D001596:Benzylamines; C586246:Cimbi-36; D005464:Fluorobenzenes; D010627:Phenethylamines; D010880:Piperidines; D044402:Receptor, Serotonin, 5-HT2A; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D014316:Tritium; D012701:Serotonin; C079049:volinanserin
keywords: 5-HT2A receptor; Cimbi-36; MDL-100907; agonist; antagonist; binding assays; frontal cortex; hippocampus; individual response; stress