title: The structure of SAV1646 from Staphylococcus aureus belonging to a new `ribosome-associated' subfamily of bacterial proteins.
abstract: The crystal structure of the SAV1646 protein from the pathogenic microorganism Staphylococcus aureus has been determined at 1.7 Å resolution. The 106-amino-acid protein forms a two-layer sandwich with α/β topology. The protein molecules associate as dimers in the crystal and in solution, with the monomers related by a pseudo-twofold rotation axis. A sequence-homology search identified the protein as a member of a new subfamily of yet uncharacterized bacterial `ribosome-associated' proteins with at least 13 members to date. A detailed analysis of the crystal protein structure along with the genomic structure of the operon containing the sav1646 gene allowed a tentative functional model of this protein to be proposed. The SAV1646 dimer is assumed to form a complex with ribosomal proteins L21 and L27 which could help to complete the assembly of the large subunit of the ribosome.
authors: Institute of Protein Research, Russian Academy of Sciences, Puschino 142290, Moscow Region, Russian Federation.
authors: Yuri N
authors: YN
authors: Chirgadze
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Teresa E
authors: TE
authors: Clarke
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Vladimir
authors: V
authors: Romanov
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Gera
authors: G
authors: Kisselman
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Jean
authors: J
authors: Wu-Brown
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Maria
authors: M
authors: Soloveychik
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Tiffany S Y
authors: TS
authors: Chan
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Roni D
authors: RD
authors: Gordon
authors: Hauptman-Woodward Medical Research Institute, IMCA-CAT, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA.
authors: Kevin P
authors: KP
authors: Battaile
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Emil F
authors: EF
authors: Pai
authors: Campbell Family Institute for Cancer research, Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2C4, Canada.
authors: Nickolay Y
authors: NY
authors: Chirgadze
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D012269:Ribosomal Proteins / Q000378:metabolism; D016415:Sequence Alignment; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C034661:ribosomal proteins L27; C060232:ribosomal protein L21
keywords: SAV1646; Staphylococcus aureus; ribosome-associated protein subfamily; α/β protein