title: Amplification of chromosome 8 genes in lung cancer.
abstract: Chromosomal alterations are frequent events in lung carcinogenesis and usually display regions of focal amplification containing several overexpressed oncogenes. Although gains and losses of chromosomal loci have been reported copy number changes of the individual genes have not been analyzed in lung cancer. In this study 22 genes were analyzed by MLPA in tumors and matched normal tissue samples from 82 patients with non-small cell lung cancer. Gene amplifications were observed in 84% of the samples. Chromosome 8 was found to harbor the most frequent copy number alterations. The most frequently amplified genes were ZNF703, PRDM14 and MYC on chromosome 8 and the BIRC5 gene on chromosome 17. The frequency of deletions were much lower and the most frequently deleted gene was ADAM9. Amplification of the ZNF703, PRDM14 and MYC genes were highly correlated suggesting that the genes displaying high copy number changes on chromosome 8 collaborate during lung carcinogenesis.
authors: 1. Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
authors: Onur
authors: O
authors: Baykara
authors: 1. Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
authors: Burak
authors: B
authors: Bakir
authors: 1. Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
authors: Nur
authors: N
authors: Buyru
authors: 2. Department of Chest Surgery, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
authors: Kamil
authors: K
authors: Kaynak
authors: 3. Department of Basic Oncology, I.U. Oncology Institute, Istanbul University, Turkey.
authors: Nejat
authors: N
authors: Dalay
keywords: Oncogenes; copy number changes; lung cancer