title: A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells.
abstract: Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, p75(NTR), S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin not only promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Qian
authors: Q
authors: Chen
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Zhijian
authors: Z
authors: Zhang
authors: Department of Orthopedics, the Third Affiliated Hospital of Suzhou University, Changzhou, China.
authors: Jinbo
authors: J
authors: Liu
authors: School of Pharmacology, Jiangsu University, Zhenjiang, China.
authors: Qinghua
authors: Q
authors: He
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Yuepeng
authors: Y
authors: Zhou
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Genbao
authors: G
authors: Shao
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Xianglan
authors: X
authors: Sun
authors: Department of Chemical Engineering, University of Ottawa, Ottawa, Ontario, Canada.
authors: Xudong
authors: X
authors: Cao
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Aihua
authors: A
authors: Gong
authors: Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors: Ping
authors: P
authors: Jiang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry / Q000502:physiology; D016023:Integrins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009297:Nasal Mucosa / Q000166:cytology*; D009414:Nerve Growth Factors / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*
chemical_list: D003470:Culture Media; D016023:Integrins; D009414:Nerve Growth Factors; D005337:Fibrin
keywords: Schwann-like cells; ectomesenchymal stem cells; fibrin; myelinating phenotype