title: Genetic engineering of Geobacillus spp.
abstract: Members of the genus Geobacillus are thermophiles that are of great biotechnological importance, since they are sources of many thermostable enzymes. Because of their metabolic versatility, geobacilli can be used as whole-cell catalysts in processes such as bioconversion and bioremediation. The effective employment of Geobacillus spp. requires the development of reliable methods for genetic engineering of these bacteria. Currently, genetic manipulation tools and protocols are under rapid development. However, there are several convenient cloning vectors, some of which replicate autonomously, while others are suitable for the genetic modification of chromosomal genes. Gene expression systems are also intensively studied. Combining these tools together with proper techniques for DNA transfer, some Geobacillus strains were shown to be valuable producers of recombinant proteins and industrially important biochemicals, such as ethanol or isobutanol. This review encompasses the progress made in the genetic engineering of Geobacillus spp. and surveys the vectors and transformation methods that are available for this genus.
authors: Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, M. K. Ciurlionio 21/27, Vilnius LT-03101, Lithuania. Electronic address: ruta.kananaviciute@gf.vu.lt.
authors: Rūta
authors: R
authors: Kananavičiūtė
authors: Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, M. K. Ciurlionio 21/27, Vilnius LT-03101, Lithuania.
authors: Donaldas
authors: D
authors: Čitavičius
mesh_terms: D000440:Butanols; D000431:Ethanol; D015870:Gene Expression; D005818:Genetic Engineering; D005821:Genetic Techniques; D005822:Genetic Vectors; D056506:Geobacillus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D000440:Butanols; D011994:Recombinant Proteins; D000431:Ethanol; C040507:isobutyl alcohol
keywords: Expression system; Genetic engineering; Genetic transformation; Geobacillus; Shuttle vector