title: Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay.
abstract: BACKGROUND
  Ochratoxin A (OTA), a widely distributed mycotoxin produced by certain species of Aspergillus and Penicillium, has been identified as a carcinogenic, hepatotoxic, teratogenic, nephrotoxic and immunotoxic toxin. To reduce the risk of OTA contamination, a rapid, inexpensive, suitable and on-site assay for its detection is required.
  
  
  RESULTS
  In this study a dot immunogold filtration assay (DIGFA) of OTA on high-flow nitrocellulose membrane was developed. Firstly colloidal gold was synthesized and colloidal gold-polyclonal antibody (PcAb) conjugates against OTA were prepared at the optimal colloidal gold-labeled pH value and package amount. Then the colloidal gold-PcAb conjugates were used to develop the OTA DIGFA. The results demonstrated a visual detection limit of approximately 10 ng mL(-1) OTA. In addition, this method had no cross-reaction with zearalenone, aflatoxin B1 or citrinin.
  
  
  CONCLUSIONS
  These results indicated that the developed DIGFA could be applied for the actual detection of samples without complicated steps.
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors: Weifeng
authors: W
authors: Chen
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors: Yucui
authors: Y
authors: Jin
authors: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, China.
authors: Aiping
authors: A
authors: Liu
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors: Xiaohong
authors: X
authors: Wang
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|State Key Laboratory of Agro-microbiology of China, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors: Fusheng
authors: F
authors: Chen
mesh_terms: D000906:Antibodies; D003101:Collodion; D005374:Filtration; D005506:Food Contamination / Q000032:analysis*; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D008567:Membranes, Artificial; D009183:Mycotoxins / Q000032:analysis; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D017979:Gold Colloid; D008567:Membranes, Artificial; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D003101:Collodion
keywords: DIGFA; OTA; colloidal gold; on-site assay