title: The expression of Mas-receptor of the renin-angiotensin system in the human eye.
abstract: OBJECTIVE
  The local renin-angiotensin system has been held to be expressed in many organs, including the eye. It has an important role in the regulation of local fluid homeostasis, cell proliferation, fibrosis, and vascular tone. Mas-receptor (Mas-R) is a potential receptor acting mainly opposite to the well-known angiotensin II receptor type 1. The aim of this study was to determine if Mas-R is expressed in the human eye.
  
  
  METHODS
  Seven enucleated human eyes were used in immunohistochemical detection of Mas-R and its endogenous ligand angiotensin (1-7) [Ang(1-7)]. Both light microscopy and immunofluorescent detection methods were used. A human kidney preparation sample was used as control.
  
  
  RESULTS
  The Mas-R was found to have nuclear localization, and localized in the retinal nuclear layers and in the structures of the anterior segment of the eye. A cytoplasmic immunostaining pattern of Ang(1-7) was found in the inner and outer nuclear and plexiform layers of the retina and in the ciliary body.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report showing Mas-R expression in the human eye. Its localization suggests that it may have a role in physiological and pathological processes in the anterior part of the eye and in the retina.
authors: Department of Ophthalmology, Tampere University Hospital, P.O. Box 2000, 33521, Tampere, Finland, anu.vaajanen@fimnet.fi.
authors: A
authors: A
authors: Vaajanen
authors: G
authors: G
authors: Kalesnykas
authors: H
authors: H
authors: Vapaatalo
authors: H
authors: H
authors: Uusitalo
mesh_terms: D000803:Angiotensin I / Q000378:metabolism*; D000869:Anterior Eye Segment / Q000378:metabolism*; D002829:Choroid / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000502:physiology*; D012160:Retina / Q000378:metabolism*
chemical_list: D008024:Ligands; D010446:Peptide Fragments; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D000803:Angiotensin I; C118790:angiotensin I (1-7)