title: Separation of five compounds from leaves of Andrographis paniculata (Burm. f.) Nees by off-line two-dimensional high-speed counter-current chromatography combined with gradient and recycling elution.
abstract: An off-line two-dimensional high-speed counter-current chromatography method combined with gradient and recycling elution mode was established to isolate terpenoids and flavones from the leaves of Andrographis paniculata (Burm. f.) Nees. By using the solvent systems composed of n-hexane/ethyl acetate/methanol/water with different volume ratios, five compounds including roseooside, 5,4'-dihydroxyflavonoid-7-O-β-d-pyranglucuronatebutylester, 7,8-dimethoxy-2'-hydroxy-5-O-β-d-glucopyranosyloxyflavon, 14-deoxyandrographiside, and andrographolide were successfully isolated. Purities of these isolated compounds were all over 95% as determined by high-performance liquid chromatography. Their structures were identified by UV, mass spectrometry, and (1) H NMR spectroscopy. It has been demonstrated that the combination of off-line two-dimensional high-speed counter-current chromatography with different elution modes is an efficient technique to isolate compounds from complex natural product extracts.
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
authors: Li
authors: L
authors: Zhang
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
authors: Qi
authors: Q
authors: Liu
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
authors: Jingang
authors: J
authors: Yu
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
authors: Hualiang
authors: H
authors: Zeng
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
authors: Shujing
authors: S
authors: Jiang
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha, China.|Collaborative Innovation Center of Resource-conserving and Environment-friendly Society and Ecological Civilization, Changsha, China.
authors: Xiaoqing
authors: X
authors: Chen
mesh_terms: D035685:Andrographis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: C477957:5,4'-dihydroxyflavonoid-7-O-pyranglucuronate butyl ester; C000590957:7,8-dimethoxy-2'-hydroxy-5-O-beta-D-glucopyranosyloxyflavone; D004224:Diterpenes; D047309:Flavones; D005419:Flavonoids; D005960:Glucosides; D005965:Glucuronates; D010936:Plant Extracts; C030419:andrographolide; C036456:14-deoxyandrographoside
keywords: Andrographis paniculata (Burm. f.) Nees; Gradient elution; High-speed counter-current chromatography; Recycling elution