title: F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression.
abstract: OBJECTIVE
  Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice.
  
  
  METHODS
  PANDER overexpression was achieved by tail-vein injection of recombinant Ad-PANDER and Ad-GFP injected mice served as a control. The TG metabolism in both groups were compared.
  
  
  RESULTS
  Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes. The triglyceride (TG) content of both liver and adipose tissue was significantly decreased in Ad-PANDER mice (liver: 6.16±1.89 mg/g vs. control 14.95±2.27 mg/g, P<0.05; adipose: 39.31±1.99 mg/100mg vs. 47.22±2.21 mg/100mg, P<0.05). The free fatty acid (FFA) content of adipose tissue in Ad-PANDER mice was also decreased (1.38±0.18 mg/g vs. 2.77±0.31 mg/g, P<0.01). The investigation of key enzymes of triglyceride hydrolysis and FFA oxidation in liver and adipose tissue showed that p-HSL/HSL was significantly increased and that DGAT1 gene and protein expression were significantly reduced in the liver of PANDER-overexpressing mice. PKA phosphorylation was also significantly increased in the livers of Ad-PANDER mice. No differences in ATGL, CPT1, ACOX1, or DGAT2 expression were observed.
  
  
  CONCLUSIONS
  Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship. The mechanisms by which this occurs remain to be elucidated.
authors: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Xiaoqing
authors: X
authors: Mo
authors: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Chijiao
authors: C
authors: Yang
authors: Department of Pharmacology, School of Medicine, Sun Yat-sen University, No.74 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Xuelan
authors: X
authors: Wang
authors: Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 E. Fowler Avenue, BSF 206, Tampa, FL33620-5550, United States of America.
authors: Brant R
authors: BR
authors: Burkhardt
authors: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Yangbin
authors: Y
authors: Li
authors: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Haipeng
authors: H
authors: Xia
authors: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.
authors: Xiaopei
authors: X
authors: Cao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D059305:Diet, High-Fat; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics; D008055:Lipids / Q000097:blood; D008066:Lipolysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D010766:Phosphorylation; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D016207:Cytokines; D008055:Lipids; C504749:PANDER protein, mouse; D014280:Triglycerides; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase; D017868:Cyclic AMP-Dependent Protein Kinases