title: Diverse fungi associated with partial irregular heartwood of Dalbergia odorifera.
abstract: Dalbergia odorifera T. Chen is a medium-sized evergreen tree that produces purple-brown heartwood called JiangXiang in traditional Chinese medicine, the formation process of which takes several decades. In this study, a standard culture method was used to isolate fungi from the wounded and normal stems of D. odorifera aiming to investigate the difference between the two types of wood. To characterize the spatial colonisation of endophytic fungi, an anatomical study was undertaken using the two different types of wood of D. odorifera. A total of 320 wood segments were placed on PDA plates and 87 fungal isolates were obtained. Only two fungi were isolated from the healthy white wood tissue, whereas 85 fungi were found in the purple-brown wounded-wood tissues. The two isolates from 160 white healthy wood tissues were assigned to Bionectriaceae sp., and the rest in wounded wood tissues were analyzed to 12 fungal species, indicating both a high fungal diversity and colonization rate in the purple-brown wounded wood. There was a difference in fungal species composition between coloured and white wood samples collected from the same tree. Eutypa sp. was the most commonly isolated species in the purple-brown wounded wood.
authors: 1] Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, China [2] College of Food and Biological engineering, Xuchang University, Xuchang, 461000, China.
authors: Sisheng
authors: S
authors: Sun
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, China.
authors: Xu
authors: X
authors: Zeng
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, China.
authors: Dawei
authors: D
authors: Zhang
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, China.
authors: Shunxing
authors: S
authors: Guo
mesh_terms: D004271:DNA, Fungal / Q000032:analysis; D035782:Dalbergia / Q000382:microbiology*; D060026:Endophytes; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014934:Wood / Q000033:anatomy & histology / Q000382:microbiology
chemical_list: D004271:DNA, Fungal