title: Dexamethasone Controlled Release on TGF-β1 Treated Vocal Fold Fibroblasts.
abstract: OBJECTIVE
  Corticosteroids may be beneficial in treating vocal fold scarring. Current drug delivery methods do not permit controlled corticosteroid release. Here we investigate the effects of poly-lactic-co-glycolic acid (PLGA) microparticles loaded with the corticosteroid dexamethasone in reducing collagen synthesis and inflammation in vocal fold fibroblasts treated with and without TGF-β1.
  
  
  METHODS
  Experimental, in vitro study.
  
  
  METHODS
  PLGA microparticles of differing molecular weight and terminating moieties were synthesized using a hydrogel template method. The release of dexamethasone was characterized from these microparticles over 4 days. Based on the release studies, ester-terminated low molecular weight PLGA microparticles were loaded with dexamethasone and applied to TGF-β1 treated vocal fold fibroblasts for 4 days. Quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to assess the effects of released dexamethasone on collagen synthesis and inflammatory mediators.
  
  
  RESULTS
  COL3A1 and COL1A2 were significantly down-regulated after exposure to ester-terminated low molecular weight PLGA microparticles loaded with dexamethasone. The loaded microparticles also reduced interleukin-6 synthesis.
  
  
  CONCLUSIONS
  These data show promise in using a PLGA microparticle-based delivery system to control dexamethasone release over 4 days. Our findings lay the groundwork for developing more effective treatments for vocal fold scarring.
authors: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
authors: Aaron M
authors: AM
authors: Kosinski
authors: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
authors: Jewel M
authors: JM
authors: Pothen
authors: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
authors: Alyssa
authors: A
authors: Panitch
authors: Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, USA msivasankar@purdue.edu.
authors: M Preeti
authors: MP
authors: Sivasankar
mesh_terms: D001672:Biocompatible Materials; D002478:Cells, Cultured; D002921:Cicatrix / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003692:Delayed-Action Preparations; D003907:Dexamethasone / Q000008:administration & dosage*; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000008:administration & dosage; D006801:Humans; D019344:Lactic Acid; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016133:Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000187:drug effects* / Q000378:metabolism; D064706:Vocal Cord Dysfunction / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014827:Vocal Cords / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005938:Glucocorticoids; D053773:Transforming Growth Factor beta1; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003907:Dexamethasone; D003094:Collagen
keywords: PLGA microparticles; dexamethasone; drug delivery; vocal fold scar