title: Occurrence of Giardia and Cryptosporidium in wild birds in Galicia (Northwest Spain).
abstract: Faecal samples were obtained from 433 wild birds being treated in wildlife recovery centres in Galicia (Northwest Spain), between February 2007 and September 2009. The birds belonged to 64 species representing 17 different orders. Giardia cysts and Cryptosporidium oocysts were detected by an immunofluorescence antibody test and identified at the molecular level by established PCR-sequencing methods. The overall prevalence of Giardia was 2·1% and that of Cryptosporidium, 8·3%. To our knowledge, this is the first description of Giardia sp. in Tyto alba and Caprimulgus europaeus; and of Cryptosporidium sp. in Apus apus, Athene noctua, C. europaeus, Falco tinnunculus, Morus bassanus, Parabuteo unicinctus and Strix aluco. Furthermore, the first PCR-sequence confirmed detection of Giardia duodenalis assemblage B in, Buteo buteo, Coturnix coturnix and Pica pica; G. duodenalis assemblage D in Garrulus glandarius; and G. duodenalis assemblage F in Anas platyrhynchos; Cryptosporidium parvum in Accipiter nisus, B. buteo, Milvus migrans, Pernis apivorus and P. pica; and Cryptosporidium meleagridis in Streptopelia turtur. The study findings demonstrate the wide spread of Giardia and Cryptosporidium between wild birds.
authors: Laboratory of Parasitology,Department of Microbiology and Parasitology,Faculty of Pharmacy,University of Santiago de Compostela,Campus Vida,15782 Santiago de Compostela,A Coruña,Spain.
authors: Aurora
authors: A
authors: Reboredo-Fernández
authors: Laboratory of Parasitology,Department of Microbiology and Parasitology,Faculty of Pharmacy,University of Santiago de Compostela,Campus Vida,15782 Santiago de Compostela,A Coruña,Spain.
authors: Elvira
authors: E
authors: Ares-Mazás
authors: Department of Infectious,Parasitic and Immunomediated Diseases,Istituto Superiore di Sanità,Viale Regina Elena 299,00161,Rome,Italy.
authors: Simone M
authors: SM
authors: Cacciò
authors: Laboratory of Parasitology,Department of Microbiology and Parasitology,Faculty of Pharmacy,University of Santiago de Compostela,Campus Vida,15782 Santiago de Compostela,A Coruña,Spain.
authors: Hipólito
authors: H
authors: Gómez-Couso
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001717:Birds / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D033661:Oocysts / Q000502:physiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan
keywords: Cryptosporidium; Galicia (Northwest Spain); Giardia; immunofluorescence microscopy; molecular characterization; wild birds