title: Super-resolution optical microscopy of lipid plasma membrane dynamics.
abstract: Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence correlation spectroscopy) on an optical (confocal) microscope is a popular tool for investigating such dynamics. Unfortunately, its full applicability is constrained by the limited spatial resolution of a conventional optical microscope. The present chapter depicts the combination of optical super-resolution STED (stimulated emission depletion) microscopy with FCS, and why it is an important tool for investigating molecular membrane dynamics in living cells. Compared with conventional FCS, the STED-FCS approach demonstrates an improved possibility to distinguish free from anomalous molecular diffusion, and thus to give new insights into lipid-protein interactions and the traditional lipid 'raft' theory.
authors: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, U.K.
authors: Christian
authors: C
authors: Eggeling
mesh_terms: D004058:Diffusion; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013109:Sphingomyelins / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; C483858:phosphatidylethanolamine; C017251:1,2-oleoylphosphatidylcholine