title: Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.
abstract: BACKGROUND
  Restenosis is a common adverse event of endovascular procedures and troubles cardiologists. However, the mechanism underlying restenosis is still not fully understood. To evaluate whether disheveled-1 (Dvl-1) is involved in the Wnt4/β-catenin signaling pathway to participate in the mechanisms of vascular restenosis.
  
  
  METHODS
  Rat model of balloon-injured carotid artery was established and atorvastatin was used to treat artery injury. Vascular smooth muscle cells (VSMC) were isolated from rats and cultured in DMEM exposed to AngII. Down-regulation and overexpression of Dvl-1 were conducted in cells to explore the role underlying its effects on VSMC proliferation and collagen expression. Adenovirus with overexpressing Dvl-1 was injected into rats to evaluate the role of Dvl-1 in artery injury rats.
  
  
  RESULTS
  The results in vivo found that Wnt4, Dvl-1 and β-catenin expression as well as collagen volume fraction (CVF) in injured artery were significantly increased. The results in vitro showed that Dvl-1 overexpression reversed the treatment effects of atorvastatin on VSMCs proliferation and collagen expression. It was also canceled by overexpressing Dvl-1 that the decrease of β-catenin protein treated with atorvastatin in cells exposed to AngII. In addition, treated artery injury rats with atorvastatin, the group with injection of Ad-Dvl-1 had higher levels of intima thickness, intimal/medial area ratio and CVF.
  
  
  CONCLUSIONS
  Dvl-1 was probably a key regulator in the pathway of wnt4/β-catenin to take part in the vascular restenosis partly, and Dvl-1 is a potential gene to anti- restenosis.
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Junyi
authors: J
authors: Hua
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Yun
authors: Y
authors: Xu
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Yuzhou
authors: Y
authors: He
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Xuhong
authors: X
authors: Jiang
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Wu
authors: W
authors: Ye
authors: Department of Cardiology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine Hangzhou 310006, Zhengjiang, China.
authors: Zhimin
authors: Z
authors: Pan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D015153:Blotting, Western; D020212:Carotid Artery Injuries / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D000072261:Dishevelled Proteins; D006083:Graft Occlusion, Vascular / Q000378:metabolism*; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D060449:Wnt Signaling Pathway / Q000502:physiology; D060528:Wnt4 Protein / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C495268:Ctnnb1 protein, rat; D000072261:Dishevelled Proteins; C000606580:Dvl1 protein, rat; D010750:Phosphoproteins; D060528:Wnt4 Protein; C555583:Wnt4 protein, rat; D051176:beta Catenin
keywords: Restenosis; disheveled-1; rat; statin; vascular smooth muscle cells; wnt; β-catenin