title: Bone regeneration of hydroxyapatite/alumina bilayered scaffold with 3 mm passage-like medullary canal in canine tibia model.
abstract: The aim of this study was to evaluate the bone regeneration of hydroxyapatite (HA)/alumina bilayered scaffold with a 3 mm passage-like medullary canal in a beagle tibia model. A porous HA/alumina scaffold was fabricated using a polymeric template-coating technique. HA/alumina scaffold dimensions were 10 mm in outer diameter, 20 mm in length, and with either a 3 mm passage or no passage. A 20 mm segmental defect was induced using an oscillating saw through the diaphysis of the beagle tibia. The defects of six beagles were filled with HA/alumina bilayered scaffolds with a 3 mm passage or without. The segmental defect was fixated using one bone plate and six screws. Bone regeneration within the HA/alumina scaffolds was observed at eight weeks after implantation. The evaluation of bone regeneration within the scaffolds after implantation in a beagle tibia was performed using radiography, computerized tomography (CT), micro-CT, and fluorescence microscopy. New bone successfully formed in the tibia defects treated with 3 mm passage HA/alumina scaffolds compared to without-passage HA/alumina scaffolds. It was concluded that the HA/alumina bilayered scaffold with 3 mm passage-like medullary canal was instrumental in inducing host-scaffold engraftment of the defect as well as distributing the newly formed bone throughout the scaffold at 8 weeks after implantation.
authors: Xenotransplantation Research Center, Department of Microbiology and Immunology, Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
authors: Jong Min
authors: JM
authors: Kim
authors: Korea Textile Development Institute, Daegu 703-702, Republic of Korea.
authors: Jun Sik
authors: JS
authors: Son
authors: Department of Veterinary Surgery, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Seong Soo
authors: 0000-0002-8957-0354
authors: SS
authors: Kang
authors: Veterinary Medical Center, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors: Gonhyung
authors: G
authors: Kim
authors: Veterinary Medical Center, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors: Seok Hwa
authors: SH
authors: Choi
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology*; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D004285:Dogs; D017886:Durapatite / Q000494:pharmacology*; D053208:Kaplan-Meier Estimate; D013977:Tibia / Q000187:drug effects* / Q000293:injuries; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017886:Durapatite; D000537:Aluminum Oxide