title: Self-assemblied nanocomplexes based on biomimetic amphiphilic chitosan derivatives for protein delivery.
abstract: A bio-inspired nanocarrier was developed for protein delivery based on biodegradable amphiphilic chitosan derivative (DCA-PCCs) with hydrophilic cell membrane mimic phosphorylcholine (PC) and hydrophobic deoxycholic acid (DCA) moieties, which was synthesized via the combination of Atherton-Todd reaction and carbodiimide coupling reaction. Using bovine serum albumin (BSA) as model protein, it was found that DCA-PCCs with suitable degree of substitution of PC and DCA moieties can load proteins by forming nanocomplexes via a solvent evaporation method. The physicochemical characteristics of BSA/DCA-PCCs nanocomplexes were investigated by Zetasizer, atomic force microscopy (AFM) and Fourier-transform infrared (FT-IR) spectroscopy. In vitro biological evaluation revealed BSA/DCA-PCCs nanocomplexes as blank DCA-PCCs nanoparticles had excellent cytocompatibility and hemocompatibility mainly due to the presence of cell membrane mimic phosphorylcholine. BSA release results suggested BSA/DCA-PCCs nanocomplexes showed a sustained release behavior following first order exponential decay kinetics. The results indicated DCA-PCCs provided a promising approach for effectively delivering therapeutic proteins.
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
authors: Minming
authors: M
authors: Wu
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
authors: Hongwei
authors: H
authors: Dong
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
authors: Kai
authors: K
authors: Guo
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China. Electronic address: tzengronga@jnu.edu.cn.
authors: Rong
authors: R
authors: Zeng
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
authors: Mei
authors: M
authors: Tu
authors: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
authors: Jianhao
authors: J
authors: Zhao
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry* / Q000633:toxicity; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D006461:Hemolysis / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D012997:Solvents; D003840:Deoxycholic Acid; D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D048271:Chitosan
keywords: Biomimetic; Chitosan; Nanocomplexes; Protein delivery; Self-assembly