title: A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species.
abstract: Amoebiasis caused by Entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. Despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. The aim of this study was to develop a new diagnostic tool to differentiate E.histolytica from the morphologically undistinguishable E.dispar and E.moshkovskii. We developed a specific, fast and simple PCR-RFLP method that was able to accurately differentiate experimentally-obtained restriction patterns from the three Entamoeba species. This new method could prove useful for clinical and epidemiological purposes.
authors: Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras. Electronic address: gustavo.fontecha@unah.edu.hn.
authors: Gustavo A
authors: GA
authors: Fontecha
authors: Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras.
authors: Kimberly
authors: K
authors: García
authors: Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras.
authors: María Mercedes
authors: MM
authors: Rueda
authors: Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras.
authors: Wilfredo
authors: W
authors: Sosa-Ochoa
authors: Microbiology Research Institute, Microbiology School, National Autonomous University of Honduras (UNAH), Ciudad Universitaria, J1, 4th fl., Tegucigalpa, Honduras; Department of Health Sciences, Brock University, St. Catharines, ON, Canada.
authors: Ana Lourdes
authors: AL
authors: Sánchez
authors: Microbiology and Parasitology Department, Faculty of Medical Sciences, Infectious Diseases Research Center, UNAN-León, León, Nicaragua.
authors: Byron
authors: B
authors: Leiva
mesh_terms: D015252:Deoxyribonucleases, Type II Site-Specific; D003937:Diagnosis, Differential; D004747:Entamoeba / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: C027908:endodeoxyribonuclease DdeI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases
keywords: E.dispar; E.moshkovskii; Entamoeba histolytica; PCR-RFLP