title: Cardiovascular effects in vitro of a polysaccharide from Salvia miltiorrhiza.
abstract: A polysaccharide (SMP1) was isolated from the roots of Salvia miltiorrhiza. This study is designed to investigate whether SMP1 prevents H9c2 cells from hydrogen peroxide (H2O2)-induced apoptosis. The present study showed that exposure of H9c2 cells to 100mM H2O2 for 24h caused a significant increase in cell death and apoptosis, but pretreatment with SMP1 eliminated H2O2-induced apoptotic cell death. Furthermore, pretreatment with SMP1 significantly prevented the mitochondria disruption, cytochrome c release, the rise of the ratio between proapoptotic Bax and antiapoptotic Bcl-2 protein expression, and caspase-3 activation in H9c2 cells upon H2O2 stimulation. Moreover, the decline of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities together with the elevation of malondialdehyde (MDA) in PC12 cells exposed to H2O2 were remarkably reversed to normal levels by pretreatment with SMP1. These results suggest that SMP1 protects H9c2 cells from H2O2-induced apoptosis through inhibition of mitochondrial dysfunction, inactivation of caspase-3 cascade and enhancement of antioxidant capacity.
authors: Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinzheng Street No. 183, Shapingba District, Chongqing 400037, China.
authors: Zhao-Hua
authors: ZH
authors: Geng
authors: Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinzheng Street No. 183, Shapingba District, Chongqing 400037, China.
authors: Lan
authors: L
authors: Huang
authors: Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinzheng Street No. 183, Shapingba District, Chongqing 400037, China.
authors: Ming-Bao
authors: MB
authors: Song
authors: Department of Cardiology, The Second Affiliated Hospital, Third Military Medical University, Xinzheng Street No. 183, Shapingba District, Chongqing 400037, China. Electronic address: mingysong@126.com.
authors: Yao-Ming
authors: YM
authors: Song
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis; D002317:Cardiovascular Agents / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides
keywords: Apoptosis; H9c2 cells; Oxidative stress; Polysaccharides; Salvia miltiorrhiza