title: [Analysis of EML4-ALK gene fusion mutation in patients  with non-small cell lung cancer].
abstract: BACKGROUND
  Non-small cell lung cancer (NSCLC) is the main type of lung cancer, and the related locus mutation detection research has become a hot direction of molecular targeted therapy, studying on gene mutation status of echinodem microtubule associated protein like 4-Anaplastic lymphoma kinase (EML4-ALK) and epidermal growth factor receptor (EGFR), detecting the sensitivity of EML4-ALK gene fusion and gene mutation of EGFR.
  
  
  METHODS
  EML4-ALK gene fusion in 85 cases of paraffin embedded tumor tissue and adjacent lung tissue was detected with the application of immunohistochemistry (IHC), Scorpions amplification refractory mutation system (Scorpions ARMS) fluorescence quantitative PCR and fluorescence in situ hybridization (FISH) technology, and EGFR gene in 18, 19, 20 and 21 exon mutation status was detected with the application of ARMS method.
  
  
  RESULTS
  In 115 cases of NSCLC, IHC showed 32 cases with ALK (D5F3) expression, the expression rate was 27.8%; ARMS showed 27 cases with EML4-ALK fusion gene mutation, the mutation detection rate was 23.5%; 53 cases were detected with EGFR mutation, the mutation rate was 46%. While FISH showed 23 cases with EML4-ALK fusion gene mutation, the detection rate was 20%, slightly lower than the ARMS detection results, suggesting that ARMS more sensitive.
  
  
  CONCLUSIONS
  The application of IHC, ARMS fluorescence quantitative PCR and FISH technology can make a rapid and accurate evaluation of EML4-ALK gene fusion.
authors: Department of Pathology, Fuzhou General Hospital of NanJing Military Rejion, Fuzhou 350025, China.
authors: Xuzhou
authors: X
authors: Wang
authors: Department of Cardiothoracic Surgery, Fuzhou General Hospital of NanJing Military Rejion, Fuzhou 350025, China.
authors: Weisheng
authors: W
authors: Chen
authors: Department of Pathology, Fuzhou General Hospital of NanJing Military Rejion, Fuzhou 350025, China.
authors: Yinghao
authors: Y
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D050939:Gene Fusion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D059645:Mutation Rate; D015514:Oncogene Proteins, Fusion / Q000235:genetics*
chemical_list: C522504:EML4-ALK fusion protein, human; D015514:Oncogene Proteins, Fusion; D066246:ErbB Receptors