title: Clinical characteristics of women diagnosed with carcinoma who tested positive for cervical and anal high-risk human papillomavirus DNA and E6 RNA.
abstract: High-risk human papillomavirus (hrHPV) is an essential cause of cervical carcinoma and is also strongly related to anal cancer development. The hrHPV E6 oncoprotein plays a major role in carcinogenesis. We aimed to evaluate the frequency of hrHPV DNA and E6 oncoprotein in the anuses of women with cervical carcinoma. We analyzed 117 women with cervical cancer and 103 controls for hrHPV and the E6 oncogene. Positive test results for a cervical carcinoma included 66.7 % with hrHPV-16 and 7.7 % with hrHPV-18. One case tested positive for both HPV variants (0.9 %). The samples from the anal canal were positive for HPV-16 in 59.8 % of the cases. Simultaneous presence of HPV in the cervix and anal canal was found in 53.8 % of the cases. Regarding expression of E6 RNA, positivity for HPV-16 in the anal canal was found in 21.2 % of the cases, positivity for HPV-16 in the cervix was found in 75.0 %, and positivity for HPV-18 in the cervix was found in 1.9 %. E6 expression in both the cervix and anal canal was found in 19.2 % of the cases. In the controls, 1 % tested positive for HPV-16 and 0 % for HPV-18. Anal samples from the controls showed a hrHPV frequency of 4.9 % (only HPV16). The presence of hrHPV in the anal canal of women with cervical cancer was detected at a high frequency. We also detected E6 RNA expression in the anal canal of women with cervical cancer, suggesting that these women are at risk for anal hrHPV infection.
authors: Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil.
authors: Carlos A R
authors: CA
authors: Veo
authors: Sarhan S
authors: SS
authors: Saad
authors: José Humberto T G
authors: JH
authors: Fregnani
authors: Cristovam
authors: C
authors: Scapulatempo-Neto
authors: Audrey Tieko
authors: AT
authors: Tsunoda
authors: Júlio César Possati
authors: JC
authors: Resende
authors: Adriana Tarlá
authors: AT
authors: Lorenzi
authors: Allini
authors: A
authors: Mafra
authors: Claudia
authors: C
authors: Cinti
authors: Ismael Dale
authors: ID
authors: Cotrim
authors: Luciana Albina Reis
authors: LA
authors: Rosa
authors: Cristina Mendes
authors: CM
authors: de Oliveira
authors: Toni Ricardo
authors: TR
authors: Martins
authors: Cristiane
authors: C
authors: Centrone
authors: José Eduardo
authors: JE
authors: Levi
authors: Adhemar
authors: A
authors: Longatto-Filho
mesh_terms: D000328:Adult; D000368:Aged; D001003:Anal Canal / Q000473:pathology / Q000821:virology*; D063646:Carcinogenesis / Q000235:genetics*; D005260:Female; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012367:RNA, Viral; D012097:Repressor Proteins