title: Immune gene expression for diverse haemocytes derived from pacific white shrimp, Litopenaeus vannamei.
abstract: In this study, diverse haemocytes from Pacific white shrimp Litopenaeus vannamei were spread by flow cytometer sorting system. Using the two commonly flow cytometric parameters FSC and SSC, the haemocytes could be divided into three populations. Microscopy observation of L. vannamei haemocytes in anticoagulant buffer revealed three morphologically distinct cell types designated as granular cell, hyaline cell and semigranular cell. Immune genes, which includes prophenoloxidase (proPO), lipopolysaccharide-β-glucan binding protein (LGBP), peroxinectin, crustin, lysozyme, penaeid-3a and transglutaminase (TGase), expressed from different haemocyte were analysed by quantitative real time PCR (qPCR). Results from the mRNA expression was estimated by relative level of each gene to β-actin gene. Finally, the seven genes could be grouped by their dominant expression sites. ProPO, LGBP and peroxinectin were highly expressed in granular cells, while LGBP, crustin, lysozyme and P-3a were highly expressed in semigranular cells and TGase was highly expressed in hyaline cells. In this study, L. vannamei haemocytes were firstly grouped into three different types and the immune related genes expression in grouped haemocytes were estimated.
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, ROC.
authors: Chih-Chiu
authors: CC
authors: Yang
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, ROC.
authors: Chung-Lun
authors: CL
authors: Lu
authors: College of Life Science, National Taiwan University, Taipei, Taiwan, ROC.
authors: Sherwin
authors: S
authors: Chen
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, ROC. Electronic address: wlliao@ccms.ntu.edu.tw.
authors: Wen-Liang
authors: WL
authors: Liao
authors: College of Life Science, National Taiwan University, Taipei, Taiwan, ROC. Electronic address: snchen@ntu.edu.tw.
authors: Shiu-Nan
authors: SN
authors: Chen
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000166:cytology / Q000276:immunology*; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D060829:Arthropod Proteins; D012333:RNA, Messenger
keywords: Granular cell; Hyaline cell; Immune gene; L. vannamei; Semigranular cell