title: Primary retinal cultures as a tool for modeling diabetic retinopathy: an overview.
abstract: Experimental models of diabetic retinopathy (DR) have had a crucial role in the comprehension of the pathophysiology of the disease and the identification of new therapeutic strategies. Most of these studies have been conducted in vivo, in animal models. However, a significant contribution has also been provided by studies on retinal cultures, especially regarding the effects of the potentially toxic components of the diabetic milieu on retinal cell homeostasis, the characterization of the mechanisms on the basis of retinal damage, and the identification of potentially protective molecules. In this review, we highlight the contribution given by primary retinal cultures to the study of DR, focusing on early neuroglial impairment. We also speculate on possible themes into which studies based on retinal cell cultures could provide deeper insight.
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Andrea
authors: 0000-0002-2986-9290
authors: A
authors: Matteucci
authors: GB Bietti Eye Foundation IRCCS, Via Livenza, 3, 00198 Rome, Italy.
authors: Monica
authors: M
authors: Varano
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Cinzia
authors: C
authors: Mallozzi
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Lucia
authors: L
authors: Gaddini
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Marika
authors: 0000-0003-2944-5444
authors: M
authors: Villa
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Sara
authors: 0000-0002-1582-4294
authors: S
authors: Gabrielli
authors: Department of Technology and Health, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Giuseppe
authors: G
authors: Formisano
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Flavia
authors: F
authors: Pricci
authors: Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.
authors: Fiorella
authors: 0000-0001-7202-0391
authors: F
authors: Malchiodi-Albedi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003930:Diabetic Retinopathy; D006801:Humans; D051379:Mice; D008954:Models, Biological; D011817:Rabbits; D051381:Rats