title: Toscana virus meningitis case in Switzerland: an example of the ezVIR bioinformatics pipeline utility for the identification of emerging viruses.
abstract: Toscana virus (TOSV) represents a frequent cause of viral meningitis in the Mediterranean Basin that remains neglected in neighbouring countries. We report a documented TOSV meningitis case in a traveller returning from Tuscany to Switzerland. While routine serological and PCR assays could not discriminate between TOSV and Sandfly fever Naples virus infection, a high-throughput sequencing performed directly on the cerebrospinal fluid specimen and analysed with the ezVIR pipeline provided an unequivocal viral diagnostic. TOSV could be unequivocally considered as the aetiological agent, proving the potential of ezVIR to improve standard diagnostics in cases of infection with uncommon or emerging viruses.
authors: Swiss National Reference Centre for Emerging Viral Infections, University of Geneva Hospitals, Geneva, Switzerland; Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland. Electronic address: samuel.cordey@hcuge.ch.
authors: S
authors: S
authors: Cordey
authors: Swiss National Reference Centre for Emerging Viral Infections, University of Geneva Hospitals, Geneva, Switzerland; Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: M
authors: M
authors: Bel
authors: Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland; Swiss Institute of Bioinformatics, Geneva, Switzerland.
authors: T J
authors: TJ
authors: Petty
authors: University of Geneva Medical School and Genomics Platform, Geneva, Switzerland.
authors: M
authors: M
authors: Docquier
authors: Swiss National Reference Centre for Emerging Viral Infections, University of Geneva Hospitals, Geneva, Switzerland; Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: L
authors: L
authors: Sacco
authors: Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: L
authors: L
authors: Turin
authors: Swiss National Reference Centre for Emerging Viral Infections, University of Geneva Hospitals, Geneva, Switzerland; Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: P
authors: P
authors: Cherpillod
authors: Laboratory of Bacteriology, University Hospitals of Geneva & Medical School, Geneva, Switzerland.
authors: S
authors: S
authors: Emonet
authors: Internal Medicine Department, University Hospitals of Geneva & Medical School, Geneva, Switzerland.
authors: M
authors: M
authors: Louis-Simonet
authors: Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland; Swiss Institute of Bioinformatics, Geneva, Switzerland; Imperial College London, South Kensington Campus, London, UK.
authors: E M
authors: EM
authors: Zdobnov
authors: Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: J
authors: J
authors: Ambrosioni
authors: Swiss National Reference Centre for Emerging Viral Infections, University of Geneva Hospitals, Geneva, Switzerland; Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Geneva, Switzerland; University of Geneva Medical School, Geneva, Switzerland.
authors: L
authors: L
authors: Kaiser
mesh_terms: D000293:Adolescent; D002044:Bunyaviridae Infections / Q000175:diagnosis* / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000821:virology; D019295:Computational Biology; D006801:Humans; D008297:Male; D008581:Meningitis / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D029301:Sandfly fever Naples virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013557:Switzerland; D055815:Young Adult
keywords: Diagnostic, ezVIR; Toscana virus; high-throughput sequencing; meningitis