title: Protein binding-induced surfactant aggregation variation: a new strategy of developing fluorescent aqueous sensor for proteins.
abstract: Novel strategies of developing fluorescent sensors for proteins are highly demanded. In this work, we particularly synthesized a cholesterol-derivatized pyrene probe. Its fluorescence emission is effectively tuned by the aggregation state of a cationic surfactant dodecyltrimethylammonium bromide (DTAB). The used probe/DTAB assemblies exhibit highly sensitive ratiometric responses to pepsin and ovalbumin egg (o-egg) with detection limits of 4.8 and 18.9 nM, respectively. The fluorescence changes indicate the protein-surfactant interaction leads to further aggregation of DTAB assemblies. The results from Tyndall effect and dynamic light scattering verify this assumption. The responses to pepsin and o-egg are due to their strong electrostatic or hydrophobic interaction with DTAB assemblies at pH 7.4. The present noncovalent supramolecular sensor represents a novel and simple strategy for sensing proteins, which is based on the encapsulated fluorophore probing the aggregation variation of the surfactant assemblies.
authors: Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University , Xi'an 710062, P. R. China.
authors: Wenting
authors: W
authors: Hu
authors: Liping
authors: L
authors: Ding
authors: Jianhua
authors: J
authors: Cao
authors: Lili
authors: L
authors: Liu
authors: Yuting
authors: Y
authors: Wei
authors: Yu
authors: Y
authors: Fang
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008823:Micelles; D010047:Ovalbumin / Q000032:analysis*; D010434:Pepsin A / Q000032:analysis*; D011485:Protein Binding; D011721:Pyrenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008823:Micelles; D011721:Pyrenes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D014867:Water; D010047:Ovalbumin; D002784:Cholesterol; C030984:pyrene; D010434:Pepsin A; C022806:dodecyltrimethylammonium
keywords: DTAB; pepsin; pyrene; ratiometric sensor; supramolecular assembly