title: Novosphingobium fluoreni sp. nov., isolated from rice seeds.
abstract: A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium designated strain HLJ-RS18(T), which could degrade fluorene, was isolated from rice seeds collected from Heilongjiang Province, China. Similarities of full-length of 16S rRNA gene sequences between strain HJL-RS18(T) and the type strains of the genus Novosphingobium with validly published names ranged from 93.8 to 97.1%. Phylogenetic analysis with maximum-likelihood and neighbour-joining methods revealed that strain HLJ-RS18(T) belonged to genus Novosphingobium and strain HLJ-RS18(T) formed a distinct clade to Novosphingobium chloroacetimidivorans BUT-14(T) (96.9% similarity based on 16S rRNA gene). DNA-DNA hybridization of HLJ-RS18(T) and BUT-14(T) showed a low relatedness value of 22.4 ± 0.9%, which indicated that strain HLJ-RS18(T) represents a novel species of the genus Novosphingobium. The genomic DNA G+C content of strain HLJ-RS18(T) was 62 mol%. Ubiquinone Q-10 was the major respiratory quinone. Spermidine was the predominant polyamine. Polar lipids consisted mainly of aminophospholipid, phosphatidylglycerol, phosphatidylethanolamine, phospholipid and sphingoglycolipid. The predominant fatty acid composition of HLJ-RS18(T) were summed 8 (C(18:1)ω7c and/or C(18:1)ω6c, 61.5%), C(16 : 0) (14.2%), summed 3 (C(16:1)ω7c and/or C(16:1)ω6c, 13.5%) and C14:0 2-OH (6.8%). Phylogenetic analysis, DNA-DNA hybridization, chemotaxonomic data and phenotypic characteristics support the conclusion that HLJ-RS18(T) represents a novel species within the genus Novosphingobium. Therefore, we propose the species Novosphingobium fluoreni sp. nov. with HLJ-RS18(T) ( = DSM 27568(T) = ACCC19180(T)) as the type strain.
authors: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, USA.|Beijing Engineering Research Center of Seed and Plant Health, Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Shumei
authors: S
authors: Gao
authors: Beijing Engineering Research Center of Seed and Plant Health, Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Yingjun
authors: Y
authors: Zhang
authors: Beijing Engineering Research Center of Seed and Plant Health, Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Na
authors: N
authors: Jiang
authors: Beijing Engineering Research Center of Seed and Plant Health, Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Laixin
authors: L
authors: Luo
authors: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, USA.
authors: Qing X
authors: QX
authors: Li
authors: Beijing Engineering Research Center of Seed and Plant Health, Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
authors: Jianqiang
authors: J
authors: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012639:Seeds / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine