title: MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells.
abstract: In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and moderate/poor differentiation. We show that miR-145 targets the DNA damage repair-associated gene Helicase-like transcription factor (HLTF), which is involved in radio-resistance. Moreover, miR-145 over-expression in cervical cancer cells enhances radiosensitivity in vitro and in vivo. These results indicate that targeting miR-145 may be a novel radiosensitizing strategy for cervical cancer.
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Chen
authors: C
authors: Ye
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Ning-xia
authors: NX
authors: Sun
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China; Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors: Yan
authors: Y
authors: Ma
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Qian
authors: Q
authors: Zhao
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Qing
authors: Q
authors: Zhang
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors: Chen
authors: C
authors: Xu
authors: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors: Shao-bing
authors: SB
authors: Wang
authors: Department of Medical Genetics, Second Military Medical University, Shanghai, China.
authors: Shu-han
authors: SH
authors: Sun
authors: Department of Medical Genetics, Second Military Medical University, Shanghai, China. Electronic address: wfsjz@163.com.
authors: Fang
authors: F
authors: Wang
authors: Department of Obstetrics & Gynaecology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address: liwenchzh@126.com.
authors: Wen
authors: W
authors: Li
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D011836:Radiation Tolerance; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D047368:Tumor Burden / Q000528:radiation effects; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D023041:Xenograft Model Antitumor Assays
chemical_list: D004268:DNA-Binding Proteins; C100023:HLTF protein, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors
keywords: Cervical cancer; Helicase-like transcription factor; MicroRNA-145; Microarray; Radiosensitivity