title: Isolation of multiple carbapenemase-producing Gram-negative bacilli from a patient recently hospitalized in Nigeria.
abstract: A 29-year-old female transferred to a tertiary care hospital in Winnipeg, Canada, after a prolonged period of hospitalization in Nigeria was found to be colonized with a VIM-2-producing Pseudomonas aeruginosa, a NDM-1-producing Klebsiella pneumoniae, and an OXA-181-producing Escherichia coli. Detection of carbapenemase-producing organisms from a rectal swab was accomplished by screening with chromogenic media, followed by confirmation with the Rapid CARB Screen kit (Rosco Diagnostica, Taastrup, Denmark). This case illustrates the need for clinical microbiology laboratories to have a protocol in place to screen patients for carbapenemase producers, even in countries where these organisms are rarely encountered.
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada; Department of Medicine, Section of Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address: AWalkty@dsmanitoba.ca.
authors: Andrew
authors: A
authors: Walkty
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada.
authors: Matthew
authors: M
authors: Gilmour
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada.
authors: Patricia
authors: P
authors: Simner
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Medicine, Section of Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: John M
authors: JM
authors: Embil
authors: National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
authors: David
authors: D
authors: Boyd
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
authors: Michael
authors: M
authors: Mulvey
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada.
authors: James
authors: J
authors: Karlowsky
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001431:Bacteriological Techniques; D002170:Canada; D002353:Carrier State / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006760:Hospitalization; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D009549:Nigeria; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemase; Gram-negative bacilli; NDM-1; VIM-2