title: The impact of sphingosine kinase 1 on the prognosis of hepatocellular carcinoma patients with portal vein tumor thrombus.
abstract: BACKGROUND
  Though there is considerable evidence that sphingosine kinase 1(SPHK1) plays a key role in hepatocellular carcinoma(HCC) progression, the prognostic value of SPHK1 expression in HCC with portal vein tumor thrombus (PVTT) remains unclear. Aims. The purpose of this study was to investigate the relationship of SPHK1 expression with PVTT and HCC recurrence after hepatectomy.
  
  
  METHODS
  After screening of gene expression profiling of tumor cell lines, real-time PCR and immunohistochemistry were used to investigate the SPHK1 expression in PVTT and HCC samples. The clinical data of 199 HCC patients with nonmain PVTT who underwent liver resection with curative intention were studied.
  
  
  RESULTS
  We identified SPHK1 as the most over-expressed gene in PVTT via gene expression profiling of one human PVTT cell line (CSQT-2). SPHK1 expression was an independent factor affecting survival (hazard ratio [HR] 1.799, 95% confidence interval [CI] 1.337-2.368, P < 0.001) and tumor recurrence (HR 1.451, 95% CI 1.087-1.935, P = 0.011). Patients with SPHK1 over-expression had a poorer prognosis than those with SPHK1 under-expression (P < 0.001 and P = 0.011 for survival and tumor recurrence).
  
  
  CONCLUSIONS
  SPHK1 might represent a novel and useful prognostic marker of HCC progression in patients with PVTT.
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Jie
authors: J
authors: Shi
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Yong-yong
authors: YY
authors: He
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Ju-Xian
authors: JX
authors: Sun
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Wei-Xing
authors: WX
authors: Guo
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Nan
authors: N
authors: Li
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Jie
authors: J
authors: Xue
authors: Hepatic Surgery Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University. Shanghai, 200438.
authors: Shu-Qun
authors: SQ
authors: Cheng
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000601:surgery; D045744:Cell Line, Tumor; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006498:Hepatectomy; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000150:complications / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011169:Portal Vein / Q000473:pathology / Q000601:surgery; D016016:Proportional Hazards Models; D011446:Prospective Studies; D015398:Signal Transduction; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D020246:Venous Thrombosis / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000401:mortality / Q000601:surgery; D000099157:Sphingosine Kinase
chemical_list: D014408:Biomarkers, Tumor; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase