title: 1-Aminocyclopropane-1-Carboxylate Deaminase from Pseudomonas stutzeri A1501 Facilitates the Growth of Rice in the Presence of Salt or Heavy Metals.
abstract: 1-Aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate the growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encoding ACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared with the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing the salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plants.
authors: Key Laboratory of Bio-resources and Eco-environment Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, P.R. China.|Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Yunlei
authors: Y
authors: Han
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.|Suzhou Biofleur Biochemical Technology Co. Ltd., Suzhou 215123, P.R. China.
authors: Rui
authors: R
authors: Wang
authors: Key Laboratory of Bio-resources and Eco-environment Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, P.R. China.
authors: Zhirong
authors: Z
authors: Yang
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Yuhua
authors: Y
authors: Zhan
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Yao
authors: Y
authors: Ma
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Shuzhen
authors: S
authors: Ping
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Liwen
authors: L
authors: Zhang
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Min
authors: M
authors: Lin
authors: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors: Yongliang
authors: Y
authors: Yan
mesh_terms: D019755:Carbon-Carbon Lyases / Q000235:genetics / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D017353:Gene Deletion; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D012275:Oryza / Q000254:growth & development* / Q000382:microbiology; D063245:Plant Development / Q000187:drug effects*; D044223:Pseudomonas stutzeri / Q000201:enzymology* / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism / Q000633:toxicity*; D013559:Symbiosis
chemical_list: D004785:Environmental Pollutants; D012965:Sodium Chloride; C022838:nickel chloride; D009532:Nickel; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: ACC deaminase; Biology nitrogen fixation; Ethylene; Pseudomonas stutzeri A1501; Stress