title: Complete sequence of RNA3 of Cucumber mosaic virus isolates infecting Gerbera jamesonii suggests its grouping under IB subgroup.
abstract: The complete RNA3 genome of Cucumber mosaic virus (CMV) was amplified by RT-PCR from three infected gerbera (Gerbera jamesonii) leaf samples exhibiting severe chlorotic mosaic and flower deformation symptoms. The amplicons obtained were cloned sequenced and deposited in GenBank under the accessions JN692495, JX913531 (from cv. Zingaro) and JX888093 (from cv. Silvester). These sequences shared 98-99 % identities to each other and with a strain of CMV-Banana reported from India, and 90-95 % identities with various strains of CMV reported worldwide. Phylogenetic analysis revealed their closest affinity with CMV-Banana strain, and close relationships with several other strains of CMV of subgroup IB. This study provides evidence of subgroup IB CMV causing severe chlorosis and flower deformation in two cultivars (Zingaro and Silvester) of G. jamesonii in India.
authors: Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
authors: K K
authors: KK
authors: Gautum
authors: Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
authors: R
authors: R
authors: Raj
authors: Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
authors: S
authors: S
authors: Kumar
authors: Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
authors: S K
authors: SK
authors: Raj
authors: Botanic Gardens, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
authors: R K
authors: RK
authors: Roy
authors: Department of Botany, University of Lucknow, Lucknow, 226007 India.
authors: R
authors: R
authors: Katiyar
keywords: Cucumber mosaic virus; Gerbera; IB subgroup isolates; RNA3 genome