title: Transcriptome analysis of Arabidopsis GCR1 mutant reveals its roles in stress, hormones, secondary metabolism and phosphate starvation.
abstract: The controversy over the existence or the need for G-protein coupled receptors (GPCRs) in plant G-protein signalling has overshadowed a more fundamental quest for the role of AtGCR1, the most studied and often considered the best candidate for GPCR in plants. Our whole transcriptome microarray analysis of the GCR1-knock-out mutant (gcr1-5) in Arabidopsis thaliana revealed 350 differentially expressed genes spanning all chromosomes. Many of them were hitherto unknown in the context of GCR1 or G-protein signalling, such as in phosphate starvation, storage compound and fatty acid biosynthesis, cell fate, etc. We also found some GCR1-responsive genes/processes that are reported to be regulated by heterotrimeric G-proteins, such as biotic and abiotic stress, hormone response and secondary metabolism. Thus, GCR1 could have G-protein-mediated as well as independent roles and regardless of whether it works as a GPCR, further analysis of the organism-wide role of GCR1 has a significance of its own.
authors: University School of Biotechnology, G.G.S. Indraprastha University, Sector 16 C, Dwarka, New Delhi, 110078, India.
authors: Navjyoti
authors: N
authors: Chakraborty
authors: University School of Biotechnology, G.G.S. Indraprastha University, Sector 16 C, Dwarka, New Delhi, 110078, India.
authors: Priyanka
authors: P
authors: Sharma
authors: Dept. of Plant Biology and Crop Science, Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, United Kingdom.
authors: Kostya
authors: K
authors: Kanyuka
authors: University School of Biotechnology, G.G.S. Indraprastha University, Sector 16 C, Dwarka, New Delhi, 110078, India.
authors: Ravi R
authors: RR
authors: Pathak
authors: School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
authors: Devapriya
authors: D
authors: Choudhury
authors: Dept. of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom.
authors: Richard A
authors: RA
authors: Hooley
authors: University School of Biotechnology, G.G.S. Indraprastha University, Sector 16 C, Dwarka, New Delhi, 110078, India.
authors: Nandula
authors: N
authors: Raghuram
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D006728:Hormones / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010710:Phosphates / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D064210:Secondary Metabolism / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C112029:GCR1 protein, Arabidopsis; D006728:Hormones; D010710:Phosphates; D043562:Receptors, G-Protein-Coupled