title: Cell size and the blockage of electron transfer in photosynthesis: proposed endpoints for algal assays and its application to soil alga Chlorococcum infusionum.
abstract: This study evaluated multiple endpoints of algal assays to identify sensitive and easy to use endpoints that could be applied to evaluate algal toxicity in metal-polluted soil extracts. Soil algae play an important role in trophic levels; thus, Chlorococcum infusionum was selected as the test species. Soil extracts were used because they might help identify potential soil retention and ecological hazards caused by pollutants that are present in the soil aqueous phase. The multi-endpoints measured were growth yield, photosynthetic activities, and cell viabilities. Nine parameters were measured to evaluate photosynthetic activity; namely, specific energy fluxes per quinone A-reducing photosystem II reaction center (absorption flux, trapped energy flux, electron transport flux, and dissipated energy flux per reaction center), quantum yields (maximum quantum yield of primary photochemistry, quantum yield of electron transport, quantum yield of energy dissipation, and average quantum yield of primary photochemistry), and the blockage of electron transfer from the reaction center to the quinone pool. Cell viability was evaluated by measuring cell size, cell granularity, and the autofluorescence of chlorophyll using flow cytometry. The results showed that heavy metals reduced growth yield, cell viability, and the photosynthetic activity of C. infusionum in soil extracts. Out of the 13 tested endpoints, the blockage of electron transfer from the reaction center to the quinone pool and cell size represented the most sensitive endpoints. We propose that both endpoints should be measured, along with conventional growth yield, to determine the effect of soil pollutants and to lower pollutant concentrations in soils.
authors: Department of Environmental Health Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors: Sun-Hwa
authors: SH
authors: Nam
authors: Department of Environmental Health Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: anyjoo@konkuk.ac.kr.
authors: Youn-Joo
authors: YJ
authors: An
mesh_terms: D048429:Cell Size; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism*; D004579:Electron Transport; D019216:Metals, Heavy / Q000633:toxicity*; D010788:Photosynthesis / Q000502:physiology*; D045332:Photosystem II Protein Complex; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D019216:Metals, Heavy; D045332:Photosystem II Protein Complex; D012987:Soil; D012989:Soil Pollutants; D002734:Chlorophyll
keywords: Chlorococcum infusionum; Copper; Heavy metals; Nickel; Soil algae; Soil extracts