title: Plasma and urinary glycosaminoglycans in the course of juvenile idiopathic arthritis.
abstract: OBJECTIVE
  The aim of the study was to perform analyses of plasma and urinary glycosaminoglycan isolated from juvenile idiopathic arthritis (JIA).
  
  
  RESULTS
  Chondroitin/dermatan sulfate (CS/DS), heparan sulfate/heparin (HS/H) and hyaluronic acid (HA) were evaluated in samples obtained from JIA patients before and after treatment. Electrophoretic analysis of GAGs identified the presence of CS, DS and HS/H in plasma of healthy subjects and JIA patients. CS were the predominant plasma GAGs constituent in all investigated subject. The plasma CS level in untreated patients was significantly decreased. Therapy resulted in an increase in this glycan level. However, plasma CS concentration still remained higher than in controls. Increased levels of DS and HA in untreated JIA patients were recorded. Anti-inflammatory treatment led to normalization of these parameters concentrations. Plasma and urinary concentrations of HS/H were similar in all groups of individuals. Urinary CS/DS and HA were decreased only in untreated patients.
  
  
  CONCLUSIONS
  The data presented indicate that changes in plasma and urinary glycosaminoglycan occur in the course of JIA. There are probably the expression of both local articular cartilage matrix and systemic changes in connective tissue remodeling.
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland. Electronic address: winsz@sum.edu.pl.
authors: Katarzyna
authors: K
authors: Winsz-Szczotka
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Kornelia
authors: K
authors: Kuźnik-Trocha
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Katarzyna
authors: K
authors: Komosińska-Vassev
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Grzegorz
authors: G
authors: Wisowski
authors: Department of Rheumatology, The Pediatric Center in Sosnowiec, Zapolskiej 3, 41-218 Sosnowiec, Poland.
authors: Anna
authors: A
authors: Gruenpeter
authors: Department of Rheumatology, The Pediatric Center in Sosnowiec, Zapolskiej 3, 41-218 Sosnowiec, Poland.
authors: Iwona
authors: I
authors: Lachór-Motyka
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Bogusław
authors: B
authors: Żegleń
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Wojciech
authors: W
authors: Lemski
authors: Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland.
authors: Krystyna
authors: K
authors: Olczyk
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood* / Q000628:therapy / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D002807:Chondroitin / Q000097:blood / Q000652:urine; D003871:Dermatan Sulfate / Q000097:blood / Q000652:urine; D005260:Female; D006025:Glycosaminoglycans / Q000097:blood* / Q000652:urine*; D006493:Heparin / Q000097:blood / Q000652:urine; D006497:Heparan Sulfate / Q000097:blood / Q000652:urine; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood / Q000652:urine; D008297:Male
chemical_list: D002807:Chondroitin; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D006820:Hyaluronic Acid
keywords: Chondroitin/dermatan sulfate; Heparan sulfate/heparin; Hyaluronic acid; Juvenile idiopathic arthritis