title: Association of the genetic polymorphisms in pre-microRNAs with risk of ischemic stroke in a Chinese population.
abstract: microRNA (miRNA) plays a role in the pathogenesis of ischemic stroke, and single nucleotide polymorphisms in miRNA genes may contribute to disease susceptibility. However, the effect of miR-146a, miR-196a2, and miR-499 polymorphisms on ischemic stroke susceptibility has been rarely reported. Using the TaqMan assay, we evaluated the association of hsa-miR-146a/rs2910164, hsa-miR-196a2/rs11614913, and hsa-miR-499/rs3746444 polymorphisms with the risk of ischemic stroke in a Chinese population with 531 ischemic stroke patients and 531 control subjects. Rs2910164 C/G genotypes were significantly associated with increased risk of ischemic stroke in different genetic model (homozygote comparison: OR = 2.00, 95% CI, 1.29-3.12, P = 0.002; additive model: OR = 1.35, 95% CI, 1.10-1.65, P = 0.004;dominant model: OR = 1.33, 95% CI, 1.00-1.75, P = 0.049; recessive model: OR = 1.82, 95% CI, 1.20-2.74, P = 0.004). Subjects with allele G of hsa-miR-146a/ rs2910164 also showed increased risk of ischemic stroke (OR = 1.33, 95% CI, 1.09-1.62, P = 0.005). Stratification analysis showed that the association between rs2910164 and the risk of ischemic stroke was more pronounced in subjects over 60 years old, females, non-drinkers, subjects without hypertension or diabetes mellitus. There were significant combined effects between miR-146a/rs2910164 and fasting glucose/low-density lipoprotein cholesterol levels on ischemic stroke susceptibility. However, we failed to find any association between the alleles/genotypes of rs11614913 T/C and ischemic stroke, respectively (P> 0.05). In summary, this study provides evidence that miR-146a/rs2910164 might be associated with a significantly increased risk of ischemic stroke in a Chinese population, and the combined effects between miRNA polymorphism and fasting glucose /blood lipid levels may contribute to stroke pathogenesis.
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Suli
authors: S
authors: Huang
authors: LongHua new District Center for Disease Control and Prevention, Shenzhen, China.
authors: Shiquan
authors: S
authors: Zhou
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Yanwei
authors: Y
authors: Zhang
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Ziquan
authors: Z
authors: Lv
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Shanshan
authors: S
authors: Li
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Changhui
authors: C
authors: Xie
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Yuebin
authors: Y
authors: Ke
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Pingjian
authors: P
authors: Deng
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Yijie
authors: Y
authors: Geng
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Qian
authors: Q
authors: Zhang
authors: Department of Neurology, People's Hospital of Shenzhen, Shenzhen, China.
authors: Xiaofan
authors: X
authors: Chu
authors: Department of Neurology, People's Hospital of Shenzhen, Shenzhen, China.
authors: Zhaohui
authors: Z
authors: Yi
authors: Department of Neurology, People's Hospital of Shenzhen, Shenzhen, China.
authors: Ying
authors: Y
authors: Zhang
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Tangchun
authors: T
authors: Wu
authors: Key Laboratory of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors: Jinquan
authors: J
authors: Cheng
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000453:epidemiology / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs