title: Pegylation effect of chitosan based polyplex on DNA transfection.
abstract: The aim of this study was to develop hepatocyte-targeting non-viral polymeric nono-carriers for gene delivery. Chitosan was selected as the main polymer. An asialoglycoprotein receptor recognized sugar, galactose, was introduced. The methoxy poly(ethylene glycol) (mPEG) or short chain poly(ethylene glycol) diacid (PEGd) was further grafted onto galactosylated chitosan. All polyplex possessed positive charge character. The compaction of DNA by grafted chitosan was in order of chitosan-galactose-mPEG>chitosan-galactose-PEGd>chitosan-galactose where the chitosan-galactose-mPEG and pDNA formed the most stable polyplex. The polyplex prominently enhanced DNA cellular transfection as compared to naked DNA in HepG2 cells in order of chitosan-galactose/pDNA (11.6±0.6-33.0±4.4%)>chitosan-galactose-PEGd/pDNA (12.7±2.5-15.5±3.0%)>chitosan-galactose-mPEG/pDNA (9.0±1.1-12.9±2.4%).
authors: Graduate Institute of Pharmaceutical Sciences, School of Pharmacy, National Taiwan University, Taipei 100, Taiwan; Drug Research Center, College of Medicine, National Taiwan University, Taipei 100, Taiwan. Electronic address: wjlin@ntu.edu.tw.
authors: Wen Jen
authors: WJ
authors: Lin
authors: Graduate Institute of Pharmaceutical Sciences, School of Pharmacy, National Taiwan University, Taipei 100, Taiwan.
authors: Wan Yi
authors: WY
authors: Hsu
mesh_terms: D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015316:Genetic Therapy; D056945:Hep G2 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; D011092:Polyethylene Glycols; D004247:DNA; D048271:Chitosan
keywords: Chitosan; DNA transfection; Galactose; Methoxy poly(ethylene glycol); Poly(ethylene glycol) diacid