title: [Evaluation of the rapid trehalose test for the identification of the Candida glabrata].
abstract: OBJECTIVE
  To explore a rapid and cost-effective method for identification of Candida glabrata through the comparison of two different methods, using molecular methods of sequencing as gold standard.
  
  
  METHODS
  From our clinic, 200 strains of suspected Candida glabrata were collected during the last 3 years and selected after incubation in CHROMagar Candida medium for 48 h. By comparing the results of the CHROMagar Candida medium, the identification of the rapid trehalose test for different kinds of strains were analyzed under incubation in the tubes for 3 h, 6 h, and 24 h at 37 °C and 42 °C, respectively. All the strains were identified to species level by methods of sequencing. The optimal time and temperature of the trehalose test for the identification of Candida glabrata were assessed. Two different methods, CHROMagar Candida medium and the rapid trehalose test, in identification of Candida glabrata were compared.
  
  
  RESULTS
  In all the 200 strains, Candida glabrata ferment trehalose with 3 h incubation under 42 °C were the optimal time and temperature for fermenting trehalose. The accuracy, sensitivity, and specificity of the rapid trehalose test were 99.00% (198/200), 98.66% (147/149) and 100.00% (51/51). The accuracy rate of CHROMagar Candida medium was 79.50% (159/200), the sensitivity and specificity were only 89.93% (134/149) and 49.02% (25/51), however, compared with the domestic current popular methods, the rapid trehalose test had better time efficiency ratio.
  
  
  CONCLUSIONS
  The evaluation results suggest that the rapid trehalose test has advantages in terms of operational convenience and low cost, and the results can be obtained in 3 h. Therefore, it has application value in clinical laboratory.
authors: Department of Clinical Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Chun
authors: C
authors: He
authors: Department of Clinical Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Qin
authors: Q
authors: Lin
authors: Department of Clinical Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Zhen-ying
authors: ZY
authors: Bao
authors: Department of Clinical Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Yan-hong
authors: YH
authors: Meng
authors: Department of Clinical Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Xiao-mei
authors: XM
authors: Qi
authors: Department of Oral Medicine, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Hong
authors: H
authors: Hua
authors: Department of Oral Medicine, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Zhi-ming
authors: ZM
authors: Yan
mesh_terms: D041221:Candida glabrata / Q000302:isolation & purification*; D006801:Humans; D012680:Sensitivity and Specificity; D014199:Trehalose
chemical_list: D014199:Trehalose