title: Plasma membrane H(+)-ATPase is involved in methyl jasmonate-induced root hair formation in lettuce (Lactuca sativa L.) seedlings.
abstract: Our results show that methyl jasmonate induces plasma membrane H (+) -ATPase activity and subsequently influences the apoplastic pH of trichoblasts to maintain a cell wall pH environment appropriate for root hair development. Root hairs, which arise from root epidermal cells, are tubular structures that increase the efficiency of water absorption and nutrient uptake. Plant hormones are critical regulators of root hair development. In this study, we investigated the regulatory role of the plasma membrane (PM) H(+)-ATPase in methyl jasmonate (MeJA)-induced root hair formation. We found that MeJA had a pronounced effect on the promotion of root hair formation in lettuce seedlings, but that this effect was blocked by the PM H(+)-ATPase inhibitor vanadate. Furthermore, MeJA treatment increased PM H(+)-ATPase activity in parallel with H(+) efflux from the root tips of lettuce seedlings and rhizosphere acidification. Our results also showed that MeJA-induced root hair formation was accompanied by hydrogen peroxide accumulation. The apoplastic acidification acted in concert with reactive oxygen species to modulate root hair formation. Our results suggest that the effect of MeJA on root hair formation is mediated by modulation of PM H(+)-ATPase activity.
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Changhua
authors: C
authors: Zhu
authors: Na
authors: N
authors: Yang
authors: Xiaoling
authors: X
authors: Ma
authors: Guijun
authors: G
authors: Li
authors: Meng
authors: M
authors: Qian
authors: Denny
authors: D
authors: Ng
authors: Kai
authors: K
authors: Xia
authors: Lijun
authors: L
authors: Gan
mesh_terms: D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018545:Lactuca / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D058441:Rhizosphere; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014638:Vanadates; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D006180:Proton-Translocating ATPases