title: Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease.
abstract: Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141-160 epitope peptide (EP141-160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP-EP141-160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP-EP141-160 VLP vaccine could protect most of the animals against FMDV.
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China.
authors: Yan-mei
authors: YM
authors: Dong
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China.
authors: Guo-guang
authors: GG
authors: Zhang
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China.
authors: Xiao-jun
authors: XJ
authors: Huang
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China. Electronic address: chenlg@xmu.edu.cn.
authors: Liang
authors: L
authors: Chen
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China. Electronic address: haotaichen@163.com.
authors: Hao-tai
authors: HT
authors: Chen
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology*; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D017909:Levivirus / Q000235:genetics; D051379:Mice; D009500:Neutralization Tests; D013552:Swine; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; C442501:VP1 protein, Foot-and-mouth disease virus; D058425:Vaccines, Virus-Like Particle; D014765:Viral Vaccines; D005620:Freund's Adjuvant
keywords: Foot-and-mouth disease (FMD); Immunity; Vaccine; Virus-like particles (VLP)