title: Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells.
abstract: Elevated expression of fatty acid synthase (FASN) and human epidermal growth factor receptor-2 (HER2) are observed in human osteosarcoma (OS). The aim in this study is to investigate a possible connection between FASN expression and the activity of HER2. The immunohistochemistry staining was conducted on 24 OS specimens from patients, which revealed a significant positive correlation between FASN and HER2 as well as p-HER2 protein expression. Furthermore, the human OS cell lines MG-63 and U2-OS were treated with FASN-specific RNAi Plasmid or Lapatinib (an inhibitor of HER2). The mRNA of HER2 and FASN was measured using RT-PCR. Western blot was performed to detect the protein expression of HER2, p-HER2 and FASN. The results demonstrated that HER2 modulates FASN expression, inhibition of FASN resulted in down-regulation of HER2 and p-HER2 protein in OS cells. Our findings suggested that there was positive feedback regulation between FASN and HER2 expression and phosphorylation in OS cells.
authors: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.
authors: Xin Hua
authors: XH
authors: Long
authors: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.
authors: Kai
authors: K
authors: Zhao
authors: Department of Orthopedics, The Second Affiliated Hospital of Nanchang University Jiangxi, P. R. China.
authors: Guo Mei
authors: GM
authors: Zhang
authors: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.
authors: Yang
authors: Y
authors: Zhou
authors: Department of Orthopedics, The Second Affiliated Hospital of Nanchang University Jiangxi, P. R. China.
authors: Rong Ping
authors: RP
authors: Zhou
authors: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.
authors: Zhi Li
authors: ZL
authors: Liu
authors: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.
authors: Zhi Hong
authors: ZH
authors: Zhang
mesh_terms: D015153:Blotting, Western; D054890:Fatty Acid Synthase, Type I / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D012516:Osteosarcoma / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D054890:Fatty Acid Synthase, Type I; D018719:Erb-b2 Receptor Tyrosine Kinases; C518729:FASN protein, human; C508053:ERBB2 protein, human
keywords: FASN; HER2; Osteosarcoma; positive feedback regulation