title: Microchip capillary electrophoresis: quantum dots and paramagnetic particles for bacteria immunoseparation: rapid superparamagnetic-beads-based automated immunoseparation of Zn-Proteins from Staphylococcus aureus with nanogram yield.
abstract: The emergence of drug-resistant bacteria and new or changing infectious pathogens is an important public health problem as well as a serious socioeconomic concern. Immunomagnetic separation-based methods create new possibilities for rapidly recognizing many of these pathogens. Nanomaterial-based techniques including fluorescent labeling by quantum dots as well as immunoextraction by magnetic particles are excellent tools for such purposes. Moreover, the combination with capillary electrophoresis in miniaturized microchip arrangement brings numerous benefits such as fast and rapid analysis, low sample consumption, very sensitive electrochemical and fluorescent detection, portable miniaturized instrumentation, and rapid and inexpensive device fabrication. Here the use of superparamagnetic particle-based fully automated instrumentation to isolate pathogen Staphylococcus aureus and its Zn(II)-containing proteins (Zn-proteins) is reported using a robotic pipetting system speeding up the sample preparation and enabling to analyze 48 real samples within 6 h. Cell lysis and Zn-protein extractions were obtained from a minimum of 100 cells with the sufficient yield for SDS-PAGE (several tens ng of proteins).
authors: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Zemedelska 1, 613 00, Brno, Czech Republic, European Union.
authors: Sona
authors: S
authors: Krizkova
authors: Hoai Viet
authors: HV
authors: Nguyen
authors: Maja
authors: M
authors: Stanisavljevic
authors: Pavel
authors: P
authors: Kopel
authors: Marketa
authors: M
authors: Vaculovicova
authors: Vojtech
authors: V
authors: Adam
authors: Rene
authors: R
authors: Kizek
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D018189:Immunomagnetic Separation / Q000379:methods*; D045663:Quantum Dots; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins