title: Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines.
abstract: Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.
authors: Institute of Biotechnology, Vilnius University, V. Graičiūno 8, Vilnius LT-02241, Lithuania. saulius.klimasauskas@bti.vu.lt.
authors: Rasa
authors: R
authors: Rakauskaitė
authors: Giedrė
authors: G
authors: Urbanavičiūtė
authors: Audronė
authors: A
authors: Rukšėnaitė
authors: Zita
authors: Z
authors: Liutkevičiūtė
authors: Robertas
authors: R
authors: Juškėnas
authors: Viktoras
authors: V
authors: Masevičius
authors: Saulius
authors: S
authors: Klimašauskas
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017279:Selenocysteine / Q000737:chemistry / Q000378:metabolism*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D001593:Benzyl Compounds; D011994:Recombinant Proteins; D051140:Selenoproteins; C485184:enhanced green fluorescent protein; D017279:Selenocysteine; D049452:Green Fluorescent Proteins