title: Aptamer pseudoknot-functionalized electronic sensor for reagentless and single-step detection of immunoglobulin E in human serum.
abstract: The development of electronic sensors with minimized usage of reagents and washing steps in the sensing protocols will significantly facilitate the detection of biomolecules. In this work, by using a new pseudoknot design of the aptamer probes, the construction of an electronic sensor for reagentless and single-step detection of immunoglobulin E (IgE) in human serum is described. The pseudoknot aptamer probes are self-assembled on the disposable electrode surface. The association of IgE with the aptamer probes leads to conformational changes of the pseudoknot aptamer structures and brings the redox-tags in close proximity to the electrode, resulting in amplified current response for monitoring IgE. The effects of the pseudoknot structure and the immobilization concentration of the aptamer probes on the sensor performance are evaluated. Under optimal conditions, the detection limit for IgE is estimated to be 60 pM. The sensor is also selective and can be employed to detect IgE in human serum samples. The developed sensor can achieve reagentless, washing-free and low-cost (with the disposable electrode) electrochemical detection of proteins, making this device a convenient sensing platform for the monitoring of different biomarkers when coupled with the appropriate aptamer probes.
authors: School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing 400054, People's Republic of China.
authors: Bingying
authors: B
authors: Jiang
authors: Fangzhen
authors: F
authors: Li
authors: Cuiyun
authors: C
authors: Yang
authors: Jiaqing
authors: J
authors: Xie
authors: Yun
authors: Y
authors: Xiang
authors: Ruo
authors: R
authors: Yuan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D064368:Healthy Volunteers; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D057230:Limit of Detection; D009690:Nucleic Acid Conformation
chemical_list: D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E