title: Epimedium flavonoids ameliorate neuropathological changes and increases IGF-1 expression in C57BL/6 mice exposed to cuprizone.
abstract: The cuprizone (CPZ)-induced toxic demyelinating model, characterized by the degeneration of oligodendrocytes, has been utilized to study multiple sclerosis-related lesions. The present study was designed to determine the effect of epimedium flavonoids (EF), the main component extracted from Epimedium sagittatum, on CPZ-induced neuropathological changes in the corpus callosum of C57BL/6 mice. Once we determined an EF-based protective effect on the corpus callosum, we sought to explore the underlying mechanism of this protection. To induce demyelination, 8-week-old mice were fed with 0.2% CPZ for a maximum period of 6 weeks. EF treatment for a period of 3 weeks effectively decreased the breakdown of myelin, OL loss, and oligodendrocyte precursor cell accumulation in CPZ-fed mice. In addition, EF administration significantly increased the cortical expression level of insulin-like growth factor 1 (IGF-1). This study provides the first in vivo evidence of EF-based protection against CPZ-induced neuropathological changes. Furthermore, our study suggests that upregulated IGF-1 may play a role in this protection.
authors: Clinical College of Integrative Medicine, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei, 230038, Anhui Province, People's Republic of China.
authors: Mengru
authors: M
authors: Liang
authors: Yongyan
authors: Y
authors: Chen
authors: Li
authors: L
authors: Zhang
authors: Lin
authors: L
authors: Li
authors: Guangliang
authors: G
authors: Chen
authors: Linlin
authors: L
authors: Yin
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000008:administration & dosage; D003337:Corpus Callosum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D003471:Cuprizone / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D034181:Epimedium; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D005419:Flavonoids; D010936:Plant Extracts; C519530:insulin-like growth factor-1, mouse; D003471:Cuprizone; D007334:Insulin-Like Growth Factor I