title: [Digital PCR compartmentalization I. Single-molecule detection of rare mutations].
abstract: Polymerase chain reaction based techniques have been widely used in laboratory settings. Several applications in oncology, virology or prenatal diagnosis require highly sensitive detection methods, which cannot be achieved with conventional techniques. Digital PCR (dPCR) was developed from the association of PCR and limiting dilution procedures. It is based on the compartmentalization of DNA molecules in small volumes. Controlling the size and the content of each compartment is crucial to obtain a high sensitivity with a single molecule resolution. Microfluidics offers promising tools to isolate DNA fragments such as microdroplets, microchambers or microwells with volumes ranging from few picoliters to nanoliters. The review provides an overview of recent developments of microfluidics dPCR platforms and how this technology can influence the management of cancer patients.
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Karla
authors: K
authors: Perez-Toralla
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France - Droplets membranes and interfaces, Max Planck institute for dynamics and self-organization, Am Fassberg 17, D-37077 Göttingen, Allemagne.
authors: Deniz
authors: D
authors: Pekin
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Jean-François
authors: JF
authors: Bartolo
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Fanny
authors: F
authors: Garlan
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Philippe
authors: P
authors: Nizard
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Pierre
authors: P
authors: Laurent-Puig
authors: Droplets membranes and interfaces, Max Planck institute for dynamics and self-organization, Am Fassberg 17, D-37077 Göttingen, Allemagne - Soft micro systems, CNRS, université de Bordeaux, CRPP, UPR 8641, 115, avenue Schweitzer, 33600 Pessac, France.
authors: Jean-Christophe
authors: JC
authors: Baret
authors: Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
authors: Valérie
authors: V
authors: Taly
mesh_terms: D003201:Computers; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D005787:Gene Frequency; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*