title: Penicillium commune metabolic profile as a promising source of antipathogenic natural products.
abstract: Penicillium is an important genus of ascomycetous fungi in the environment and in food and drug production. This paper aims to investigate statins and antipathogenic natural products from a Penicillium commune environmental isolate. Fractions (F1, F2, F3 and F4) were obtained from an ethyl acetate extract. Direct insertion probe/electron ionisation/ion trap detection mass spectrometry (MS and MS/MS) identified lovastatin (1) in F1, while GC-MS showed that 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) was the main constituent of F2 (49.34%). F4 presented 3 (16.38%) as an analogue of 2 and their known structures were similar to that of an autoinducer-signal. F1 produced a significant decrease in the Pseudomonas aeruginosa biofilms, which is the main cause of bacterial pathogenicity. F2 and F4 were effective against Staphylococcus aureus biofilms, but when F2 was associated with oxacillin, it showed an important activity against both bacteria. These novel results suggest that P. commune INTA1 is a new source of promising antipathogenic products.
authors: a Facultad de Bioquímica, Química y Farmacia, Instituto de Química Orgánica, Universidad Nacional de Tucumán , Ayacucho 471, Tucumán 4000 , Argentina.|b Cátedra de Micología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán , Ayacucho 471, Tucumán 4000 , Argentina.
authors: Lorena
authors: L
authors: Diblasi
authors: c LISA-CONICET , Ayacucho 471, Tucumán 4000 , Argentina.
authors: Federico
authors: F
authors: Arrighi
authors: b Cátedra de Micología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán , Ayacucho 471, Tucumán 4000 , Argentina.
authors: Julio
authors: J
authors: Silva
authors: a Facultad de Bioquímica, Química y Farmacia, Instituto de Química Orgánica, Universidad Nacional de Tucumán , Ayacucho 471, Tucumán 4000 , Argentina.|c LISA-CONICET , Ayacucho 471, Tucumán 4000 , Argentina.|d INQUINOA-CONICET , Ayacucho 471, Tucumán 4000 , Argentina.
authors: Alicia
authors: A
authors: Bardón
authors: a Facultad de Bioquímica, Química y Farmacia, Instituto de Química Orgánica, Universidad Nacional de Tucumán , Ayacucho 471, Tucumán 4000 , Argentina.
authors: Elena
authors: E
authors: Cartagena
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry* / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D055442:Metabolome; D015394:Molecular Structure; D010407:Penicillium / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products
keywords: DIP/EI/ITD mass spectrometry (MS and MS/MS); GC-MS analysis; Penicillium commune metabolic profile; biofilms; lovastatin; pyrrolopyrazines