title: High-resolution magic-angle-spinning NMR spectroscopy of intact tissue.
abstract: High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy is a nondestructive technique that is used to obtain the metabolite profile of a tissue sample. This method requires minimal sample preparation. However, it is important to handle the sample with care and keep it frozen during preparation to minimize degradation. Here, we describe a typical protocol for HR-MAS analysis of intact tissue. We also include examples of typical pulse sequence programs and quantification methods that are used today.
authors: Department of Circulation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors: Guro F
authors: GF
authors: Giskeødegård
authors: Maria D
authors: MD
authors: Cao
authors: Tone F
authors: TF
authors: Bathen
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D002138:Calibration; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome; D009584:Nitrogen; D012015:Reference Standards; D013696:Temperature
chemical_list: D009584:Nitrogen