title: UBE3C promotes growth and metastasis of renal cell carcinoma via activating Wnt/β-catenin pathway.
abstract: Renal cell carcinoma (RCC) is the most common primary malignancy of the kidney and one of the most lethal genitourinary malignancies. Clear-cell renal cell carcinoma (ccRCC) has an extremely poor prognosis because of a high potential for tumor growth, vascular invasion, metastasis and recurrence. Unfortunately, the mechanism of RCC growth and metastasis is not well understood. In this report, we for the first time demonstrated ubiquitin protein ligase E3C (UBE3C) as a driving factor for RCC growth and metastasis. UBE3C expression was increased in ccRCC tissues compared with adjacent normal tissues. ccRCC patients with high UBE3C protein expression in tumors were associated with significantly worse postoperative survival. Knockdown of UBE3C expression in ACHN cells inhibited cell proliferation, migrations and invasiveness in vitro while overexpression of UBE3C in 786-O cells exerted the opposite effects. UBE3C up-regulated β-catenin protein levels and promoted β-catenin nuclear accumulation, leading to the activation of the Wnt/β-catenin signal pathway in RCC cells. Collectively, these observations suggest that UBE3C plays an important role in RCC development and progression, and UBE3C may be a novel target for prevention and treatment of ccRCC.
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Ji Ling
authors: JL
authors: Wen
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Xiao Fei
authors: XF
authors: Wen
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Rong Bing
authors: RB
authors: Li
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Yong Chao
authors: YC
authors: Jin
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Xue Lei
authors: XL
authors: Wang
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Lan
authors: L
authors: Zhou
authors: Department of Urology, East Hospital, Tongji University School of Medicine, Shang Hai 200120, China.
authors: Hui Xing
authors: HX
authors: Chen
mesh_terms: D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008297:Male; D009362:Neoplasm Metastasis; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics; D060449:Wnt Signaling Pathway
chemical_list: C555759:UBE3C protein, human; D044767:Ubiquitin-Protein Ligases