title: Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings.
abstract: For many years it has been a major challenge to regenerate damaged tissues using synthetic or natural materials. To favor the healing processes after tendon, cornea, muscle, or brain injuries, aligned collagen-based architectures are of utmost interest. In this study, we define a novel aligned coating based on a collagen/alginate (COL/ALG) multilayer film. The coating exhibiting a nanofibrillar structure is cross-linked with genipin for stability in physiological conditions. By stretching COL/ALG-coated polydimethylsiloxane substrates, we developed a versatile method to align the collagen fibrils of the polymeric coating. Assays on cell morphology and alignment were performed to investigate the properties of these films. Microscopic assessments revealed that cells align with the stretched collagen fibrils of the coating. The degree of alignment is tuned by the stretching rate (i.e., the strain) of the COL/ALG-coated elastic substrate. Such coatings are of great interest for strategies that require aligned nanofibrillar biological material as a substrate for tissue engineering.
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Christophe
authors: C
authors: Chaubaroux
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .|3 LBBM-NHC , CHRU de Strasbourg, Strasbourg, France .
authors: Fabienne
authors: F
authors: Perrin-Schmitt
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Bernard
authors: B
authors: Senger
authors: 4 Institut de Science des Matériaux de Mulhouse , IS2M UMR 7361, CNRS - UHA, Mulhouse, France .
authors: Loïc
authors: L
authors: Vidal
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Jean-Claude
authors: JC
authors: Voegel
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .|5 Institut Charles Sadron , CNRS UPR 22, Université de Strasbourg, Strasbourg, France .|6 Institut Universitaire de France , Paris, France .
authors: Pierre
authors: P
authors: Schaaf
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Youssef
authors: Y
authors: Haikel
authors: 5 Institut Charles Sadron , CNRS UPR 22, Université de Strasbourg, Strasbourg, France .|7 Institut d'Etudes Avancées, Université de Strasbourg , Strasbourg, France .
authors: Fouzia
authors: F
authors: Boulmedais
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Philippe
authors: P
authors: Lavalle
authors: 1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .|2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
authors: Joseph
authors: J
authors: Hemmerlé
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D002417:Cattle; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D051381:Rats
chemical_list: D000464:Alginates; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D003094:Collagen