title: Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle.
abstract: A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay.
authors: Department of Biotechnology, Lund University, 22100 Lund, Sweden. Electronic address: Kinga.Zor@biotek.lu.se.
authors: Kinga
authors: K
authors: Zór
authors: Department of Biotechnology, Lund University, 22100 Lund, Sweden.
authors: Katarzyna
authors: K
authors: Dymek
authors: Department of Biochemistry and Structural Biology/Analytical Chemistry, Lund University, 22100 Lund, Sweden.
authors: Roberto
authors: R
authors: Ortiz
authors: Department of Biotechnology, Lund University, 22100 Lund, Sweden.
authors: Audrey M
authors: AM
authors: Faure
authors: Department of Biotechnology, Lund University, 22100 Lund, Sweden.
authors: Ebru
authors: E
authors: Saatci
authors: Department of Biochemistry and Structural Biology/Analytical Chemistry, Lund University, 22100 Lund, Sweden.
authors: Lo
authors: L
authors: Gorton
authors: Division of Nutritional Sciences, University of Nottingham, LE12 5RD, Nottingham, UK.
authors: Ronald
authors: R
authors: Bardsley
authors: Department of Biotechnology, Lund University, 22100 Lund, Sweden.
authors: Mihaela
authors: M
authors: Nistor
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002417:Cattle; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; C033668:calpastatin
keywords: Amperometry; Calpastatin; Immunoassay; Matrix effect; Raw meat