title: Resveratrol induces apoptosis and alters gene expression in human fibrosarcoma cells.
abstract: OBJECTIVE
  Metastatic fibrosarcomas still represent a therapeutic dilemma. Commonly used chemotherapeutic agents such as doxorubicin have been proven effective in fewer than 30% of all cases disseminated of fibrosarcoma. Elderly patients with cardiac disease are not suitable for systemic chemotherapy with doxorubicin. We therefore tested the apoptotic effects of the natural and well-tolerated compound resveratrol on human fibrosarcoma cells (HT1080).
  
  
  METHODS
  Vital, apoptotic and necrotic cells were quantified using flow cytometric analysis. Gene expression was analyzed by RNA microarrays.
  
  
  RESULTS
  Application of resveratrol induced apoptotic cell death and significantly reduced proliferation of HT1080 cells. Correspondingly, expression of apoptosis-associated genes was altered in microarray analysis.
  
  
  CONCLUSIONS
  This in vitro study demonstrates the anticancer activity of resveratrol against human fibrosarcoma cells. These results provide experimental support for in vivo trials assessing the effect of the natural polyphenol resveratrol.
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany kamran.harati@t-online.de.
authors: Kamran
authors: K
authors: Harati
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Pawel
authors: P
authors: Slodnik
authors: Visceral and General Surgery, St. Josef Hospital Ruhr University Bochum, Bochum, Germany.
authors: Ansgar Michael
authors: AM
authors: Chromik
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Ole
authors: O
authors: Goertz
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Tobias
authors: T
authors: Hirsch
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Nikolai
authors: N
authors: Kapalschinski
authors: Institute for Cell Biology (Tumor Research), University of Duisburg-Essen, Essen, Germany.
authors: Ludger
authors: L
authors: Klein-Hitpass
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Jonas
authors: J
authors: Kolbenschlag
authors: Visceral and General Surgery, St. Josef Hospital Ruhr University Bochum, Bochum, Germany.
authors: Waldemar
authors: W
authors: Uhl
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Marcus
authors: M
authors: Lehnhardt
authors: Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG University Hospital Bergmannsheil Bochum, Bochum, Germany.
authors: Adrien
authors: A
authors: Daigeler
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol
keywords: FACS; HT1080; Soft tissue sarcoma; apoptosis; chemotherapy; doxorubicin; fibrosarcoma; gene expression; necrosis; phytotherapy; proliferation; resveratrol; taurolidine