title: β-Thujaplicin modulates estrogen receptor signaling and inhibits proliferation of human breast cancer cells.
abstract: β-Thujaplicin, one of the major constituents in Chamaecyparis obtusa, has been demonstrated to exert different health beneficial efficacy, but the role of β-thujaplicin in regulating mammary tumorigenesis has not been investigated. In this study, we found that β-thujaplicin significantly suppressed the proliferation through arresting the cell cycle transition from G1 to S phase as well as inhibited the expression of cell cycle-related proteins, cyclin D1, and cyclin-dependent kinase 4 (CDK4) in MCF-7 and T47D luminal subtype breast cancer cells. In addition, estrogen receptor α (ER-α) was down-regulated by β-thujaplicin via enhanced proteolysis by ubiquitination, which led to cell growth inhibition. These results suggest that β-thujaplicin may be considered as a potent agent regulating the hormone sensitive mammary tumorigenesis.
authors: a Department of Food Science and Technology , Chung-Ang University , Anseong , South Korea.
authors: Jiwon
authors: J
authors: Ko
authors: Cheng
authors: C
authors: Bao
authors: Hyun-Chang
authors: HC
authors: Park
authors: Minchae
authors: M
authors: Kim
authors: Hyung-Kyoon
authors: HK
authors: Choi
authors: Young-Suk
authors: YS
authors: Kim
authors: Hong Jin
authors: HJ
authors: Lee
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D063646:Carcinogenesis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D039821:Monoterpenes / Q000494:pharmacology*; D059748:Proteolysis / Q000187:drug effects; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D014334:Tropolone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016588:Anticarcinogenic Agents; D047628:Estrogen Receptor alpha; D039821:Monoterpenes; D019938:Cyclin D1; D014334:Tropolone; D051358:Cyclin-Dependent Kinase 4; C009479:beta-thujaplicin
keywords: breast cancer; cyclin D1; estrogen receptor; ubiquitination; β-thujaplicin