title: Analgesic mechanism of electroacupuncture in a rat L5 spinal nerve ligation model.
abstract: The aim of this study was to investigate the analgesic mechanism of electroacupuncture (EA) in the treatment of neuropathological pain. A total of 60 Sprague-Dawley rats were randomly divided into three groups, namely the spinal nerve ligation (SNL), electroacupuncture (SNL + EA) and normal control groups, with 20 rats in each group. The up-down method was used to determine the bipedal 50% mechanical paw withdrawal threshold (PWT). The ultrastructure of the injured-side L5 nerve root (n=6) was observed by electron microscopy. The mRNA levels of brain-derived neurotrophic factor (BDNF) and purinergic receptor P2X, ligand-gated ion channel 4 (P2X4) in the spinal cord (n=14) were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The postoperative PWT of the injured-side hindpaw in the SNL group at each time point was lower than that in the control group (P<0.01); there were differences of statistical significance between the PWT values of the SNL + EA and SNL groups on postoperative days 14 and 21 (P<0.05). Postoperatively, the PWT of the hindpaw on the uninjured-side was significantly lower in the SNL group when compared with that of the control group on days 10, 14 and 21 (P<0.05). Following the EA treatment, axonal demyelination was reduced and vascular proliferation was observed within the visual field. In addition, following the EA treatment, BDNF expression levels in the spinal dorsal horn increased (P<0.05), while the expression of P2X4 was not different from that in the SNL group. EA exerted an analgesic effect on the SNL model in a time-dependent manner, and improved the blood supply to the nerve root. Following the EA treatment, the expression of P2X4 did not change significantly compared with that in the SNL group, whereas the spinal secretion of BDNF increased. However, the exact mechanism requires further study.
authors: Pain Management Center, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.
authors: Chunchun
authors: C
authors: Xue
authors: Pain Management Center, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.
authors: Lei
authors: L
authors: Xie
authors: Pain Management Center, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.
authors: Xia
authors: X
authors: Li
authors: Department of Orthopaedics and Traumatology, Jiashan Hospital of Traditional Chinese Medicine, Jiashan, Zhejiang 314100, P.R. China.
authors: Jianfeng
authors: J
authors: Cai
authors: Pain Management Center, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.
authors: Zhen
authors: Z
authors: Gu
authors: Pain Management Center, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.
authors: Kaiqiang
authors: K
authors: Wang
keywords: brain-derived neurotrophic factor; electroacupuncture; ligand-gated ion channel 4; purinergic receptor P2X; spine nerve ligation