title: Immunohistochemical expression of sex steroid hormone receptors, cell cycle regulators and angiogenesis factors in intraductal papillary mucinous neoplasms: an explorative study.
abstract: OBJECTIVE
  The objectives of our explorative study were to (i) evaluate the immunohistochemical expression of sex steroid hormone receptors (estrogen receptor a [ERα], estrogen receptor β [ERβ], progesterone receptor [PR] and androgen receptor [AR]), angiogenesis factors (vascular endothelial growth factor [VEGF] and inhibitor of differentiation/DNA synthesis 1 [Id-1]) and cell-cycle regulators (cyclin D1, p16 and p27) in intraductal papillary mucinous neoplasms (IPMNs) in comparison to normal adjacent pancreatic tissues and (ii) assess their correlation with the grade and histological sub-type of those lesions.
  
  
  METHODS
  Paraffin-embedded specimens from 12 consecutive patients with IPMNs were immunostained for the studied markers and staining quantification was assessed by an image analysis system.
  
  
  RESULTS
  AR H-score and cyclin D1 H-score were significantly higher in the IPMN lesions (0.86±0.33 vs. 0.57±0.12 in the normal tissue, p=0.010 and 0.47±0.23 vs. 0.21±0.20 in the normal tissue, p=0.019, respectively). No significant differences were noted regarding the expression of ERα, ERβ, PR, p16, p27, VEGF, Id-1 or MVD. Moreover, no significant associations were found between the expression of studied markers and grade or histological subtype.
  
  
  CONCLUSIONS
  Our study showed higher expression of AR and cyclin D1 in IPMNs compared to normal pancreatic ducts without any association between AR and cyclin D1 expression and IPMNs' grade or subtype.
authors: 3rd Surgical Clinic of George Gennimatas General Hospital, Athens, Greece dcgeorgiadou@gmail.com.
authors: Despoina
authors: D
authors: Georgiadou
authors: Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, University of Athens, Athens, Greece.
authors: Theodoros N
authors: TN
authors: Sergentanis
authors: 1st Department of Pathology, National and Kapodistrian University of Athens, Athens, Greece.
authors: Stratigoula
authors: S
authors: Sakellariou
authors: 1st Department of Propaedeutic Surgery, Hippokratio Hospital, Athens, Greece.
authors: George M
authors: GM
authors: Filippakis
authors: 1st Department of Propaedeutic Surgery, Hippokratio Hospital, Athens, Greece.
authors: Flora
authors: F
authors: Zagouri
authors: Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, University of Athens, Athens, Greece.
authors: Theodora
authors: T
authors: Psaltopoulou
authors: 1st Department of Pathology, National and Kapodistrian University of Athens, Athens, Greece.
authors: Andreas C
authors: AC
authors: Lazaris
authors: 1st Department of Pathology, National and Kapodistrian University of Athens, Athens, Greece.
authors: Efstratios
authors: E
authors: Patsouris
authors: Research Center, Hellenic Anticancer Institute, Athens, Greece.
authors: Antonia
authors: A
authors: Gounaris
authors: 1st Department of Propaedeutic Surgery, Hippokratio Hospital, Athens, Greece.
authors: George C
authors: GC
authors: Zografos
mesh_terms: D000368:Aged; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D043925:Angiogenesis Inducing Agents; D012739:Gonadal Steroid Hormones; D011956:Receptors, Cell Surface
keywords: IPMN; Sex steroid receptors; angiogenesis molecules; cell cycle regulators; image analysis