title: The volatile anesthetic sevoflurane attenuates ventilator-induced lung injury through inhibition of ERK1/2 and Akt signal transduction.
abstract: BACKGROUND
  Ventilator-induced lung injury (VILI) sustained during mechanical ventilator support is still a cause of a high rate of morbidity and mortality in intensive care units and in operating rooms. VILI is characterized by pulmonary inflammation that appears to be mediated by proinflammatory cytokines. This study investigates whether the volatile anesthetic sevoflurane has an anti-inflammatory effect that attenuates VILI.
  
  
  METHODS
  Twenty one male rabbits were anesthetized and were mechanically ventilated with 50% oxygen at a peak inspiratory pressure (PIP) of 10 cmH2O, I : E ratio of 1 : 4, and positive end expiratory pressure of 5 cmH2O. All animals were randomly assigned to one of three groups that were ventilated for 5 h with 10 cmH2O of PIP (Sham group, n = 7); 30 cmH2O of PIP (Control group, n = 7); or 30 cmH2O of PIP and 0.8 vol% sevoflurane (Sevoflurane group, n = 7). The wet/dry weight (W/D) ratio and histopathology of the lung; concentration of interleukin-8 (IL-8) in the bronchoalveolar lavage fluid; and activation of extracellular signal-regulated kinases (ERK) 1/2, p38 mitogen-activated protein kinase, and Akt were measured in the lung tissue after completing the protocol.
  
  
  RESULTS
  Histopathology indicated that the sevoflurane group showed fewer inflammatory cells and architectural changes than the control group did. The W/D ratio [(5.36 ± 0.13) versus (6.61 ± 0.20)], expression of IL-8 [(144.08 ± 14.61) versus (228.56 ± 15.13) pg/ml] and phosphorylation of ERK1/2 and Akt decreased significantly in the sevoflurane group relative to the control group.
  
  
  CONCLUSIONS
  Sevoflurane attenuates VILI in rabbits mainly by inhibiting expression of IL-8, and Sevoflurane-induced inhibition of phosphorylated ERK1/2 and Akt might be a possible pathway for protection.
authors: Department of Anesthesiology and Pain Meidicne, Chosun University Medical School, Gwangju, Korea.
authors: Sang-Hun
authors: SH
authors: Kim
authors: Department of Anesthesiology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Mei
authors: M
authors: Li
authors: Department of Anesthesiology and Pain Meidicne, Chonnam National University Medical School, Gwangju, Korea.
authors: Tae-Hee
authors: TH
authors: Pyeon
authors: Department of Anesthesiology and Pain Meidicne, Chosun University Medical School, Gwangju, Korea.
authors: Keum-Young
authors: KY
authors: So
authors: Department of Anesthesiology and Pain Meidicne, Chonnam National University Medical School, Gwangju, Korea.
authors: Sang-Hyun
authors: SH
authors: Kwak
keywords: Akt; ERK1/2; Sevoflurane; Ventilator-induced lung injury