title: In vitro inhibition of caprine herpesvirus 1 by acyclovir and mizoribine.
abstract: Caprine herpesvirus 1 (CpHV-1) infection in goats induces genital vesicular-ulcerative lesions that strictly resemble the lesions induced by herpesvirus 2 in the human host. The immunosuppressive drug Mizoribine (MIZ) was found to increase the antiviral activity of Acyclovir (ACV) against herpesvirus infections, raising interesting perspectives on new combined therapeutic strategies. In this study the anti-CpHV-1 activity in vitro of ACV alone or in combination with MIZ was characterized. When applied alone at non-toxic concentrations, ACV had a slight effect on CpHV-1 replication while in combination with MIZ a dose-dependent inhibition of the virus yield was observed with an IC50 of ACV of 28.5 µM. These findings suggest that combined therapy of ACV and MIZ is potentially exploitable in the treatment of genital infection by herpesviruses.
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. Electronic address: gabriella.elia@uniba.it.
authors: G
authors: G
authors: Elia
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: M
authors: M
authors: Camero
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: N
authors: N
authors: Decaro
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: A
authors: A
authors: Lovero
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: V
authors: V
authors: Martella
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: M
authors: M
authors: Tempesta
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: C
authors: C
authors: Buonavoglia
authors: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors: G
authors: G
authors: Crescenzo
mesh_terms: D000212:Acyclovir / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002417:Cattle; D002478:Cells, Cultured; D006566:Herpesviridae Infections / Q000188:drug therapy / Q000662:veterinary* / Q000821:virology; D012263:Ribonucleosides / Q000494:pharmacology* / Q000627:therapeutic use; D018140:Varicellovirus / Q000187:drug effects* / Q000254:growth & development; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D012263:Ribonucleosides; C010052:mizoribine; D000212:Acyclovir
keywords: Acyclovir; CpHV-1; MDBK cells; Mizoribine; Viral titration