title: Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions.
abstract: Heat shock (HS) is one of the best-studied exogenous cellular stresses. Almost all tissues, cell types, metabolic pathways and biochemical reactions are affected in greater or lesser extent by HS. However, there are some especially thermo sensible cellular types such as the mammalian male germ cells. The present study examined the role of three INDELs in conjunction with the -660G/C polymorphism located at the HSP90AA1 promoter region over the gene expression rate under HS. Specially, the -668insC INDEL, which is very close to the -660G/C transversion, is a good candidate to be implied in the transcriptional regulation of the gene by itself or in a cooperative way with this SNP. Animals carrying the genotype II-668 showed higher transcription rates than those with ID-668 (FC = 3.07) and DD-668 (FC = 3.40) genotypes for samples collected under HS. A linkage between gene expression and sperm DNA fragmentation was also found. When HS conditions were present along or in some stages of the spermatogenesis, alternative genotypes of the -668insC and -660G/C mutations are involved in the effect of HS over sperm DNA fragmentation. Thus, unfavorable genotypes in terms of gene expression induction (ID-668GC-660 and DD-668GG-660) do not produce enough mRNA (stored as messenger ribonucleoprotein particles) and Hsp90α protein to cope with future thermal stress which might occur in posterior stages when transcriptional activity is reduced and cell types and molecular processes are more sensible to heat (spermatocytes in pachytene and spermatids protamination). This would result in the impairment of DNA packaging and the consequent commitment of the events occurring shortly after fertilization and during embryonic development. In the short-term, the assessment of the relationship between sperm DNA fragmentation sensitivity and ram's fertility will be of interest to a better understanding of the mechanisms of response to HS and its consequences on animal production and reproduction performance.
authors: INIA, Carretera de La Coruña Km. 7.5, 28040, Madrid, Spain.
authors: Judit
authors: J
authors: Salces-Ortiz
authors: CERSYRA, Av. Del Vino 10, 13300, Valdepeñas, Spain.
authors: Manuel
authors: M
authors: Ramón
authors: INIA, Carretera de La Coruña Km. 7.5, 28040, Madrid, Spain.
authors: Carmen
authors: C
authors: González
authors: CERSYRA, Av. Del Vino 10, 13300, Valdepeñas, Spain.
authors: M Dolores
authors: MD
authors: Pérez-Guzmán
authors: SaBio IREC (CSIC-UCLM-JCCM) Campus Universitario S.N., 02071, Albacete, Spain.
authors: J Julián
authors: JJ
authors: Garde
authors: SaBio IREC (CSIC-UCLM-JCCM) Campus Universitario S.N., 02071, Albacete, Spain.
authors: Olga
authors: O
authors: García-Álvarez
authors: SaBio IREC (CSIC-UCLM-JCCM) Campus Universitario S.N., 02071, Albacete, Spain.
authors: Alejandro
authors: A
authors: Maroto-Morales
authors: Unidad de Tecnología en Producción Animal, CITA, 59059, Zaragoza, Spain.
authors: Jorge H
authors: JH
authors: Calvo
authors: INIA, Carretera de La Coruña Km. 7.5, 28040, Madrid, Spain.
authors: M Magdalena
authors: MM
authors: Serrano
mesh_terms: D000483:Alleles; D000818:Animals; D053938:DNA Fragmentation; D005786:Gene Expression Regulation; D005787:Gene Frequency; D059647:Gene-Environment Interaction; D008040:Genetic Linkage; D005838:Genotype; D018869:Heat-Shock Response / Q000235:genetics*; D054643:INDEL Mutation; D015810:Linkage Disequilibrium; D008297:Male; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012756:Sheep / Q000235:genetics; D013094:Spermatozoa / Q000378:metabolism*