title: Red sorrel (Hibiscus Sabdariffa) prevents the ethanol-induced deficits of Purkinje cells in the cerebellum.
abstract: OBJECTIVE
  The present study is aimed at investigating the possible protective effects of H. sabdariffa on ethanol-elicited deficits of motor coordination and estimated total number of the Purkinje cells of the cerebellums of adolescent male Wistar rats.
  
  
  METHODS
  Forty male Wistar rats aged 21 days were divided into five groups. Na/wtr group was given water orally and injected with normal saline intra peritoneally (ip). Eth/wtr group was given water orally and ethanol (ip). Another three experimental groups (Eth/Hsab) were given different dosages of H. sabdariffa and ethanol (ip). All groups were treated intermittently for the total period of treatment of two weeks. The motor coordination of rats was tested prior and subsequent to the treatments. The rats were euthanized, and their cerebellums were examined. The total number of Purkinje cells was estimated using physical fractionator method.
  
  
  RESULTS
  Upon revolving drum test, the number of falls of rats increased following ethanol treatment. There was no significant difference between the total number of falls prior and subsequent to treatment in all Eth/Hsab groups. The estimated total number of Purkinje cells in Eth/Hsab groups was higher than in Eth/wtr group.
  
  
  CONCLUSIONS
  H. sabdariffa may prevent the ethanol-induced deficits of motor coordination and estimated total number of Purkinje cells of the cerebellums in adolescent rats (Tab. 3, Fig. 1, Ref. 42).
authors: S
authors: S
authors: Suryanti
authors: G
authors: G
authors: Partadiredja
authors: J
authors: J
authors: Atthobari
mesh_terms: D000818:Animals; D002526:Cerebellar Diseases / Q000139:chemically induced / Q000188:drug therapy; D002531:Cerebellum / Q000187:drug effects* / Q000473:pathology*; D000431:Ethanol; D031584:Hibiscus; D008297:Male; D028321:Plant Preparations / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011689:Purkinje Cells / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D028321:Plant Preparations; D020011:Protective Agents; D000431:Ethanol