title: Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation.
abstract: Hepatocyte transplantation is a potential therapy for treating various liver diseases. However, oxygen shortage leading to loss of hepatocyte function becomes a limitation following hepatocyte transplantation. To overcome this problem, we developed a hybrid organoid, consisting of growth factor (GF)-immobilizable gel particles combined with hepatocytes. Benefits of the hybrid organoid were evaluated in three groups: (i) hybrid organoid consisting of cells and GF-immobilizable gel particles (HG-C); (ii) hybrid organoid consisting of cells and gel particles (G-C); and (iii) cells suspended in collagen (C-C). We found liver-specific functions of HG-C were maintained longer than in the other conditions during in vitro culture. Furthermore, after transplantation, HG-C was effective in maintaining viability of transplanted hepatocytes and promoting angiogenesis around the hepatocytes. In summary, transplantation of HG-C is a potential method for future liver tissue engineering.
authors: Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors: Jingjia
authors: J
authors: Ye
authors: Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors: Nana
authors: N
authors: Shirakigawa
authors: Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: ijima@chem-eng.kyushu-u.ac.jp.
authors: Hiroyuki
authors: H
authors: Ijima
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D003094:Collagen; D005109:Extracellular Matrix / Q000737:chemistry* / Q000637:transplantation*; D005782:Gels / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000502:physiology / Q000601:surgery; D008107:Liver Diseases / Q000601:surgery; D016031:Liver Transplantation; D019164:Liver, Artificial; D008297:Male; D018919:Neovascularization, Physiologic; D009940:Organoids / Q000737:chemistry / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen
keywords: Animal cell culture; Growth factor; Hepatocyte transplantation; Immobilization; Organoid; Tissue engineering