title: Survey of Anisakis sp. and Hysterothylacium sp. in sardines and anchovies from the North Adriatic Sea.
abstract: The occurrence of larval Anisakidae and Raphidascarididae in anchovies and sardines from the North Adriatic Sea has been estimated. Anisakis pegreffii and Hysterothylacium aduncum were reported, with low prevalence values. In brief, a total amount of 7650 fish specimens collected between September 2011 and 2012 were analysed using three different inspection analyses: a visual inspection of the coelomic cavity, an examination of the viscera exploiting the positive hydro-tropism of the larvae (modified Baermann technique) and enzymatic digestion of muscular tissue pools. Low level of infestation was reported for Anisakis sp. in both in anchovies and sardines, while higher values were reported for Hysterothylacium sp. Subsamples of nematodes collected were characterized at species level using the molecular diagnostic key based on ITS nuclear ribosomal region, and A. pegreffii and H. aduncum were identified. The low prevalence of Anisakis sp. in sardines and anchovies from the North Adriatic Sea could be related to the peculiar distribution of cetaceans and carnivorous zooplankton in the investigated region and could be used as a potential tag to define oily fishes from this specific fishing area as at low-risk for anisakiasis.
authors: Sapienza University of Rome, Department of Public Health and Infectious Diseases, P.le Aldo Moro, 5-00185 Rome, Italy. Electronic address: serena.cavallero@uniroma1.it.
authors: S
authors: S
authors: Cavallero
authors: Istituto Zooprofilattico Sperimentale delle Venezie, V.le Università, 10-35020 Legnaro, Padua, Italy.
authors: C
authors: C
authors: Magnabosco
authors: Istituto Zooprofilattico Sperimentale delle Venezie, V.le Università, 10-35020 Legnaro, Padua, Italy.
authors: M
authors: M
authors: Civettini
authors: Veterinary Public Service, AULSSn. 14, Chioggia, Venice, Italy.
authors: L
authors: L
authors: Boffo
authors: Veterinary Public Service, AULSSn. 14, Chioggia, Venice, Italy.
authors: G
authors: G
authors: Mingarelli
authors: Veterinary Public Service, AULSSn. 14, Chioggia, Venice, Italy.
authors: P
authors: P
authors: Buratti
authors: Institute for Environmental Protection and Research, ISPRA, Loc. Brondolo, Chioggia, Venice, Italy.
authors: O
authors: O
authors: Giovanardi
authors: Institute for Environmental Protection and Research, ISPRA, Via Curtatone, 3, 00185 Rome, Italy.
authors: C M
authors: CM
authors: Fortuna
authors: Istituto Zooprofilattico Sperimentale delle Venezie, V.le Università, 10-35020 Legnaro, Padua, Italy.
authors: G
authors: G
authors: Arcangeli
mesh_terms: D000818:Animals; D017129:Anisakiasis / Q000453:epidemiology* / Q000469:parasitology; D017163:Anisakis / Q000235:genetics; D017191:Ascaridida Infections / Q000453:epidemiology* / Q000469:parasitology; D001199:Ascaridoidea / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology; D005399:Fishes / Q000469:parasitology; D007814:Larva; D008522:Mediterranean Sea / Q000453:epidemiology; D015995:Prevalence; D017747:Seafood / Q000469:parasitology
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Anchovies; Anisakidosis; Anisakis; Hysterothylacium; North Adriatic Sea; Sardines