title: Rab25 upregulation correlates with the proliferation, migration, and invasion of renal cell carcinoma.
abstract: Renal cell carcinoma (RCC) is a common urological cancer with a poor prognosis. A recent cohort study revealed that the median survival of RCC patients was only 1.5 years and that <10% of the patients in the study survived up to 5 years. In tumor development, Rab GTPase are known to play potential roles such as regulation of cell proliferation, migration, invasion, communication, and drug resistance in multiple tumors. However, the correlation between Rabs expression and the occurrence, development, and metastasis of RCC remains unclear. In this study, we analyzed the transcriptional levels of 52 Rab GTPases in RCC patients. Our results showed that high levels of Rab25 expression were significantly correlated with RCC invasion classification (P < 0.01), lymph-node metastasis (P < 0.001), and pathological stage (P < 0.01). Conversely, in 786-O and A-498 cells, knocking down Rab25 protein expression inhibited cell proliferation, migration, and invasion. Our results also demonstrated that Rab25 is a target gene of let-7d, and further suggested that Rab25 upregulation in RCC is due to diminished expression of let-7d. These findings indicate that Rab25 might be a novel candidate molecule involved in RCC development, thus identifying a potential biological therapeutic target for RCC.
authors: Biomedical Analysis Center, Third Military Medical University, Chongqing, China; Chongqing Key Laboratory of Cytomics, Chongqing, China.
authors: Yuanyuan
authors: Y
authors: Li
authors: Biomedical Analysis Center, Third Military Medical University, Chongqing, China; Chongqing Key Laboratory of Cytomics, Chongqing, China.
authors: Qingzhu
authors: Q
authors: Jia
authors: Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Qian
authors: Q
authors: Zhang
authors: Biomedical Analysis Center, Third Military Medical University, Chongqing, China; Chongqing Key Laboratory of Cytomics, Chongqing, China. Electronic address: wanying_cn@163.com.
authors: Ying
authors: Y
authors: Wan
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015331:Cohort Studies; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology*; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C509763:Rab25 protein, human; C487931:mirnlet7 microRNA, human; D020691:rab GTP-Binding Proteins
keywords: Invasion; Let-7d; Migration; Proliferation; Rab25; Renal cell carcinoma