title: Evaluation of the effects of ischemic preconditioning on the hematological parameters of rats subjected to intestinal ischemia and reperfusion.
abstract: OBJECTIVE
  Intestinal ischemia/reperfusion often leads to acute lung injury and multiple organ failure. Ischemic preconditioning is protective in nature and reduces tissue injuries in animal and human models. Although hematimetric parameters are widely used as diagnostic tools, there is no report of the influence of intestinal ischemia/reperfusion and ischemic preconditioning on such parameters. We evaluated the hematological changes during ischemia/reperfusion and preconditioning in rats.
  
  
  METHODS
  Forty healthy rats were divided into four groups: control, laparotomy, intestinal ischemia/reperfusion and ischemic preconditioning. The intestinal ischemia/reperfusion group received 45 min of superior mesenteric artery occlusion, while the ischemic preconditioning group received 10 min of short ischemia and reperfusion before 45 min of prolonged occlusion. A cell counter was used to analyze blood obtained from rats before and after the surgical procedures and the hematological results were compared among the groups.
  
  
  RESULTS
  The results showed significant differences in hematimetric parameters among the groups. The parameters that showed significant differences included lymphocyte, white blood cells and granulocyte counts; hematocrit; mean corpuscular hemoglobin concentration; red cell deviation width; platelet count; mean platelet volume; plateletcrit and platelet distribution width.
  
  
  CONCLUSIONS
  The most remarkable parameters were those related to leukocytes and platelets. Some of the data, including the lymphocyte and granulocytes counts, suggest that ischemic preconditioning attenuates the effect of intestinal ischemia/reperfusion on circulating blood cells. Our work contributes to a better understanding of the hematological responses after intestinal ischemia/reperfusion and IPC, and the present findings may also be used as predictive values.
authors: Laboratory of Biochemistry and Protein Chemistry, Cell Biology Dept, University of Brasilia, Brasilia, DF, Brazil.
authors: Muhammad
authors: M
authors: Tahir
authors: Laboratory of Surgical Physiopathology (LIM-62), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors: Samina
authors: S
authors: Arshid
authors: Laboratory of Surgical Physiopathology (LIM-62), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors: Ana Maria C
authors: AM
authors: Heimbecker
authors: Laboratory of Biochemistry and Protein Chemistry, Cell Biology Dept, University of Brasilia, Brasilia, DF, Brazil.
authors: Mariana S
authors: MS
authors: Castro
authors: Laboratory of Surgical Physiopathology (LIM-62), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors: Edna Frasson de
authors: EF
authors: Souza Montero
authors: Laboratory of Surgical Physiopathology (LIM-62), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors: Belchor
authors: B
authors: Fontes
authors: Laboratory of Biochemistry and Protein Chemistry, Cell Biology Dept, University of Brasilia, Brasilia, DF, Brazil.
authors: Wagner
authors: W
authors: Fontes
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count; D001773:Blood Cells; D007422:Intestines / Q000098:blood supply* / Q000601:surgery; D007511:Ischemia / Q000097:blood*; D019194:Ischemic Preconditioning / Q000379:methods*; D007813:Laparotomy / Q000379:methods; D008297:Male; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000097:blood* / Q000517:prevention & control; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D015415:Biomarkers