title: Nogo Receptor 1 Limits Ocular Dominance Plasticity but not Turnover of Axonal Boutons in a Model of Amblyopia.
abstract: The formation and stability of dendritic spines on excitatory cortical neurons are correlated with adult visual plasticity, yet how the formation, loss, and stability of postsynaptic spines register with that of presynaptic axonal varicosities is unknown. Monocular deprivation has been demonstrated to increase the rate of formation of dendritic spines in visual cortex. However, we find that monocular deprivation does not alter the dynamics of intracortical axonal boutons in visual cortex of either adult wild-type (WT) mice or adult NgR1 mutant (ngr1-/-) mice that retain critical period visual plasticity. Restoring normal vision for a week following long-term monocular deprivation (LTMD), a model of amblyopia, partially restores ocular dominance (OD) in WT and ngr1-/- mice but does not alter the formation or stability of axonal boutons. Both WT and ngr1-/- mice displayed a rapid return of normal OD within 8 days after LTMD as measured with optical imaging of intrinsic signals. In contrast, single-unit recordings revealed that ngr1-/- exhibited greater recovery of OD by 8 days post-LTMD. Our findings support a model of structural plasticity in which changes in synaptic connectivity are largely postsynaptic. In contrast, axonal boutons appear to be stable during changes in cortical circuit function.
authors: Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
authors: Michael G
authors: MG
authors: Frantz
authors: Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
authors: Ryan J
authors: RJ
authors: Kast
authors: Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
authors: Hilary M
authors: HM
authors: Dorton
authors: Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
authors: Katherine S
authors: KS
authors: Chapman
authors: Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
authors: Aaron W
authors: AW
authors: McGee
mesh_terms: D000550:Amblyopia / Q000235:genetics / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D023882:Dominance, Ocular; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009473:Neuronal Plasticity; D009474:Neurons / Q000502:physiology; D000070816:Nogo Receptor 1 / Q000235:genetics / Q000502:physiology*; D017729:Presynaptic Terminals / Q000502:physiology*; D012683:Sensory Deprivation; D014792:Visual Acuity / Q000502:physiology; D014793:Visual Cortex / Q000166:cytology / Q000503:physiopathology*
chemical_list: D000070816:Nogo Receptor 1
keywords: axon; bouton; electrophysiology; in vivo imaging; long-term monocular deprivation; optical imaging