title: Rab11a regulates syntaxin 3 localization and microvillus assembly in enterocytes.
abstract: Rab11a is a key component of the apical recycling endosome that aids in the trafficking of proteins to the luminal surface in polarized epithelial cells. Utilizing conditional Rab11a-knockout specific to intestinal epithelial cells, and human colonic epithelial CaCo2-BBE cells with stable Rab11a knockdown, we examined the molecular and pathological impact of Rab11a deficiency on the establishment of apical cell polarity and microvillus morphogenesis. We demonstrate that loss of Rab11a induced alterations in enterocyte polarity, shortened microvillar length and affected the formation of microvilli along the lateral membranes. Rab11a deficiency in enterocytes altered the apical localization of syntaxin 3. These data affirm the role of Rab11a in apical membrane trafficking and the maintenance of apical microvilli in enterocytes.
authors: Department of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Byron C
authors: BC
authors: Knowles
authors: Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Victoria G
authors: VG
authors: Weis
authors: Department of Biological Sciences, Rutgers University, Newark, NJ 07102, USA.
authors: Shiyan
authors: S
authors: Yu
authors: Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Joseph T
authors: JT
authors: Roland
authors: Vanderbilt Ingraham Cancer Center: Cell Imaging Shared Resource, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Janice A
authors: JA
authors: Williams
authors: Department of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Gabriela S
authors: GS
authors: Alvarado
authors: Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37235, USA.
authors: Lynne A
authors: LA
authors: Lapierre
authors: Phoenix Children's Hospital and the Department of Child Health, University of Arizona College of Medicine, Phoenix, AZ 85004, USA.
authors: Mitchell D
authors: MD
authors: Shub
authors: Department of Biological Sciences, Rutgers University, Newark, NJ 07102, USA Rutgers Cancer Institute of New Jersey, Piscataway, NJ 08903, USA ngao@andromeda.rutgers.edu jim.goldenring@vanderbilt.edu.
authors: Nan
authors: N
authors: Gao
authors: Department of Cell & Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, TN 37235, USA Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37235, USA ngao@andromeda.rutgers.edu jim.goldenring@vanderbilt.edu.
authors: James R
authors: JR
authors: Goldenring
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D016764:Cell Polarity; D011992:Endosomes / Q000378:metabolism; D020895:Enterocytes / Q000648:ultrastructure*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008871:Microvilli / Q000378:metabolism*; D021381:Protein Transport; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D050765:Qa-SNARE Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
keywords: Enterocyte; Intestinal polarity; Microvilli; Rab11; Rab8; Syntaxin 3