title: Synthesis and biological evaluation of a novel MUC1 glycopeptide conjugate vaccine candidate comprising a 4'-deoxy-4'-fluoro-Thomsen-Friedenreich epitope.
abstract: The development of selective anticancer vaccines that provide enhanced protection against tumor recurrence and metastasis has been the subject of intense research in the scientific community. The tumor-associated glycoprotein MUC1 represents a well-established target for cancer immunotherapy and has been used for the construction of various synthetic vaccine candidates. However, many of these vaccine prototypes suffer from an inherent low immunogenicity and are susceptible to rapid in vivo degradation. To overcome these drawbacks, novel fluorinated MUC1 glycopeptide-BSA/TTox conjugate vaccines have been prepared. Immunization of mice with the 4'F-TF-MUC1-TTox conjugate resulted in strong immune responses overriding the natural tolerance against MUC1 and producing selective IgG antibodies that are cross-reactive with native MUC1 epitopes on MCF-7 human cancer cells.
authors: Department of Chemistry and Center of Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians University, Butenandtstraße 5-13, D-81377 Munich, Germany.
authors: Manuel
authors: M
authors: Johannes
authors: Department of Chemistry and Center of Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians University, Butenandtstraße 5-13, D-81377 Munich, Germany.
authors: Maximilian
authors: M
authors: Reindl
authors: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, Geb. 708, D-55101 Mainz, Germany.
authors: Bastian
authors: B
authors: Gerlitzki
authors: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, Geb. 708, D-55101 Mainz, Germany.
authors: Edgar
authors: E
authors: Schmitt
authors: Department of Chemistry and Center of Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians University, Butenandtstraße 5-13, D-81377 Munich, Germany.
authors: Anja
authors: A
authors: Hoffmann-Röder
keywords: MUC1; TACA; cancer immunotherapy; fluorinated carbohydrates; glycoconjugates