title: Piceatannol inhibits effector T cell functions by suppressing TcR signaling.
abstract: Piceatannol, a metabolite of resveratrol found in red wine and grapes, displays a wide spectrum of biological activity. Although the anti-oxidant, anti-inflammatory, and anti-tumorigenesis activity of piceatannol has been extensively studied, its role in the adaptive immune response has received less attention. Here we investigated the role of piceatannol, a well-known Syk inhibitor, in T cell activation, proliferation, and differentiation using isolated murine splenic T cells from C57BL/6 mice. Piceatannol treatment inhibited surface expression of CD4 and CD8 T cell activation markers CD25 and CD69, reduced production of cytokines IFNγ, IL-2, and IL-17, and suppressed proliferation of activated T cells. Moreover, piceatannol treatment significantly inhibited differentiation of CD4(+)CD25(-)CD62L(+) naïve CD4 T cells into Th1, Th2, and Th17 cells, presumably due to inhibition of TcR signaling through p-Erk, p-Akt, and p-p38. Piceatannol appears to be a useful nutritional or pharmacological biomolecule that regulates effector T cell functions such as cytokine production, differentiation, and proliferation.
authors: Department of Life Science, Hanyang University, Seoul 133-791, South Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors: Do-Hyun
authors: DH
authors: Kim
authors: Department of Biomaterials Science and Technology, Yonsei University, Seoul 120-749, South Korea; OTTOGI Research Institute, OTTOGI Corporation, Gyeonggi-do 431-070, South Korea.
authors: Yong-Gab
authors: YG
authors: Lee
authors: Department of Life Science, Hanyang University, Seoul 133-791, South Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors: Hong-Jai
authors: HJ
authors: Park
authors: Department of Life Science, Hanyang University, Seoul 133-791, South Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors: Jung-Ah
authors: JA
authors: Lee
authors: Food Safety Research Group, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, South Korea.
authors: Hyun Jung
authors: HJ
authors: Kim
authors: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea. Electronic address: jkhwang@yonsei.ac.kr.
authors: Jae-Kwan
authors: JK
authors: Hwang
authors: Department of Life Science, Hanyang University, Seoul 133-791, South Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea. Electronic address: jeminchoi@hanyang.ac.kr.
authors: Je-Min
authors: JM
authors: Choi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013267:Stilbenes / Q000494:pharmacology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D020928:Mitogen-Activated Protein Kinases; D047428:Protein Kinase Inhibitors; D011948:Receptors, Antigen, T-Cell; D013267:Stilbenes; D000099192:CD69 Antigens; C508018:Il2ra protein, mouse; C041525:3,3',4,5'-tetrahydroxystilbene
keywords: Piceatannol; T cell differentiation; T cell proliferation; T cells; TcR signaling