title: De novo DNA methyltransferase DNMT3A: Regulation of oligomeric state and mechanism of action in response to pH changes.
abstract: BACKGROUND
  The oligomeric state of the human DNMT3A is functionally important and cancer cells are known to undergo changes in pH (intracellular).
  
  
  METHODS
  Light scattering, gel filtration, and fluorescence anisotropy. Also, methylation and processivity assays.
  
  
  CONCLUSIONS
  Physiologically relevant changes in pH result in changes in DNMT3A oligomer composition which have dramatic consequences on DNMT3A function.
  
  
  CONCLUSIONS
  The pH changes which occur within cancer cells alter the oligomeric state and function of DNMT3A which could contribute to changes in genomic DNA methylation observed in vivo.
authors: Interdepartmental Program in Biomolecular Science & Engineering, University of California, Santa Barbara, CA 93106-9510, United States.
authors: Celeste
authors: C
authors: Holz-Schietinger
authors: Interdepartmental Program in Biomolecular Science & Engineering, University of California, Santa Barbara, CA 93106-9510, United States; Department of Chemistry & Biochemistry, University of California, Santa Barbara, CA 93106-9510, United States. Electronic address: reich@chem.ucsb.edu.
authors: Norbert O
authors: NO
authors: Reich
mesh_terms: D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D011487:Protein Conformation; D055503:Protein Multimerization; D013329:Structure-Activity Relationship
chemical_list: C000717967:DNMT3A protein, human; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A
keywords: Cancer; DNA methylation; DNMT3A; Oligomers; Processivity; pH