title: Increase in isolation of Burkholderia contaminans from Spanish patients with cystic fibrosis.
abstract: Species of the Burkholderia cepacia complex are associated with opportunistic infection in patients with cystic fibrosis. For years now, B. multivorans and B. cenocepacia have been the most frequently isolated species within the complex in such patients. However, between 2008 and 2012, the overall incidence of these species in Spain (17.7% and 12.5% respectively) was overtaken by that of B. contaminans (36.5%). The population structure of B. contaminans isolates from Spanish patients with cystic fibrosis was analysed using multilocus sequence typing and pulsed-field gel electrophoresis (PFGE). Three major known sequence types (ST102, ST404 and ST482) and a new one (ST771) were identified among 59 isolates. In addition, PFGE detected 17 pulsotypes. Susceptibility to antibiotics was examined using the Etest. Cotrimoxazole and ceftazidime were the most active antibiotics against B. contaminans, inhibiting growth in 88% and 86% of the isolates, respectively. In addition, this species showed less resistance to most of the antibiotics tested than did either B. multivorans or B. cenocepacia isolates recovered from similar Spanish patients.
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. Electronic address: mjmedina@isciii.es.
authors: M J
authors: MJ
authors: Medina-Pascual
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: S
authors: S
authors: Valdezate
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: G
authors: G
authors: Carrasco
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: P
authors: P
authors: Villalón
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: N
authors: N
authors: Garrido
authors: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: J A
authors: JA
authors: Saéz-Nieto
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019121:Burkholderia Infections / Q000453:epidemiology*; D003550:Cystic Fibrosis / Q000150:complications*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D018410:Pneumonia, Bacterial / Q000453:epidemiology*; D013030:Spain / Q000453:epidemiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial susceptibility; Burkholderia contaminans, cystic fibrosis; genetic diversity; multilocus sequence typing; pulsed field gel electrophoresis