title: Expression of enzymes for the usage in food and feed industry with Pichia pastoris.
abstract: The methylotrophic yeast Pichia pastoris is an established protein expression host for the production of industrial enzymes. This yeast can be grown to very high cell densities and produces high titers of recombinant protein, which can be expressed intercellularly or be secreted to the fermentation medium. P. pastoris offers some advantages over other established expression systems especially in protein maturation. In food and feed production many enzymatically catalyzed processes are reported and the demand for new enzymes grows continuously. For instance the unique catalytic properties of enzymes are used to improve resource efficiency, maintain quality, functionalize food, and to prevent off-flavors. This review aims to provide an overview on recent developments in heterologous production of enzymes with P. pastoris and their application within the food sector.
authors: Faculty of Biology and Chemistry, Justus Liebig University Giessen, Giessen Germany; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen Germany.
authors: Sebastian C
authors: SC
authors: Spohner
authors: Faculty of Biology and Chemistry, Justus Liebig University Giessen, Giessen Germany; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen Germany.
authors: Hagen
authors: H
authors: Müller
authors: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen Germany.
authors: Hendrich
authors: H
authors: Quitmann
authors: Faculty of Biology and Chemistry, Justus Liebig University Giessen, Giessen Germany; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen Germany; Department of Chemical Engineering, Kansas State University, Manhattan USA. Electronic address: peter.czermak@kmub.thm.de.
authors: Peter
authors: P
authors: Czermak
mesh_terms: D004798:Enzymes / Q000096:biosynthesis* / Q000235:genetics; D005285:Fermentation; D019649:Food Industry; D010843:Pichia / Q000235:genetics / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development
chemical_list: D004798:Enzymes; D011994:Recombinant Proteins
keywords: Food and feed industry; Fructooligosaccharides; Phytase; Pichia pastoris; Protein expression; Yeast