title: Redox-dependent translocation of the heat shock transcription factor AtHSFA8 from the cytosol to the nucleus in Arabidopsis thaliana.
abstract: The hypothesis is tested that some heat stress transcription factors (HSFs) are activated after formation of inter- or intramolecular disulfide bonds. Based on in silico analyses we identified conserved cysteinyl residues in AtHSFA8 that might function as redox sensors in plants. AtHSFA8 represents a redox-sensitive transcription factor since upon treatment of protoplasts with H2O2 YFP-labeled HSFA8 was translocated to the nucleus in a time-dependent manner. Site-directed mutagenesis of the conserved residues Cys24 and Cys269 blocked translocation of HSFA8 to the nucleus. The findings concur with a model where HSFA8 functions as redox sensing transcription factor within the stress-responsive transcriptional network.
authors: Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany.
authors: Miriam
authors: M
authors: Giesguth
authors: Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany.
authors: Arne
authors: A
authors: Sahm
authors: Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany.
authors: Swen
authors: S
authors: Simon
authors: Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany. Electronic address: karl-josef.dietz@uni-bielefeld.de.
authors: Karl-Josef
authors: KJ
authors: Dietz
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D017124:Conserved Sequence; D003600:Cytosol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D021381:Protein Transport; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000597330:HSFA8 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors; D006861:Hydrogen Peroxide
keywords: AtHSFA8; Cytoplasmic-nuclear shuttling; Fluorescence resonance energy transfer; Heat stress transcription factor; Reactive oxygen species; Redox-regulation