title: Protective effects of cyanidin-3-rutinoside against monosaccharides-induced protein glycation and oxidation.
abstract: Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, is present in various fruits and vegetables as a colorant. C3R has been well characterized and demonstrated a number of biological activities attributed to its antioxidant properties. The present study compared the effectiveness of C3R against monosaccharide-induced protein glycation and oxidation in vitro using bovine serum albumin (BSA).The results demonstrated that C3R (0.125-1.00 mM) inhibited the formation of fluorescent AGEs in ribose-glycated BSA (2-52%), fructose-glycated BSA (81-93%), glucose-glycated BSA (30-74%) and galactose-glycated BSA (6-79%).Correspondingly, C3R (1.00 mM) decreased the level of N(ɛ)-(carboxymethyl) lysine (56-86%) in monosaccharide-induced glycation in BSA. C3R also reduced the level of fructosamine, β-amyloid cross structure, protein carbonyl content as well as the depletion of thiol in BSA/monosaccharide system. In summary, C3R might offer a new promising antiglycation agent for the prevention of diabetic complications by inhibiting AGE formation and oxidation-dependent protein damage.
authors: Program in Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand; Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Thavaree
authors: T
authors: Thilavech
authors: Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok 10330, Thailand; Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Sathaporn
authors: S
authors: Ngamukote
authors: CSIRO Food& Nutrition Flagship, Adelaide SA 5000, Australia.
authors: Mahinda
authors: M
authors: Abeywardena
authors: Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok 10330, Thailand; Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: sirichai.a@chula.ac.th.
authors: Sirichai
authors: S
authors: Adisakwattana
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry; D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D005453:Fluorescence; D019270:Fructosamine / Q000032:analysis; D006031:Glycosylation / Q000187:drug effects; D009005:Monosaccharides / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D012710:Serum Albumin, Bovine; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D000872:Anthocyanins; D009005:Monosaccharides; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D019270:Fructosamine; C428983:cyanidin 3-rutinoside
keywords: Advanced glycation end products; Cyanidin-3-rutinoside; Monosaccharide