title: Preliminary X-ray crystallographic analysis of an engineered variant of human chimera-type galectin-3 with a shortened N-terminal domain.
abstract: How lectins translate sugar-encoded information into cellular effects not only depends on glycan recognition. Other domains of the protein can contribute to the functional profile of a lectin. Human galectin-3 (Gal-3), an adhesion/growth-regulatory galectin, is composed of three different domains and is thus called a chimera-type protein. In addition to the carbohydrate-recognition domain, this lectin encompasses an N-terminal domain consisting of a peptide harbouring two phosphorylation sites and nine non-triple-helical collagen-like repeats. This region plays an as yet structurally undefined role in Gal-3 aggregation and ligand recognition. To date, crystallization of full-length Gal-3 has not been achieved. With the aim of providing structural insights into this modular organization, a Gal-3 variant was crystallized maintaining the terminal peptide and three of the nine collagen-like repeats. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 94.04, b = 97.96, c = 236.20 Å, and diffracted to a resolution of 3.3 Å.
authors: Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors: Andrea
authors: A
authors: Flores-Ibarra
authors: Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors: Federico M
authors: FM
authors: Ruiz
authors: Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, 80539 München, Germany.
authors: Sabine
authors: S
authors: Vértesy
authors: Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, 80539 München, Germany.
authors: Sabine
authors: S
authors: André
authors: Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, 80539 München, Germany.
authors: Hans-Joachim
authors: HJ
authors: Gabius
authors: Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors: Antonio
authors: A
authors: Romero
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D037502:Galectin 3 / Q000737:chemistry*; D006801:Humans; D050505:Mutant Proteins / Q000737:chemistry*; D015202:Protein Engineering; D017434:Protein Structure, Tertiary
chemical_list: D037502:Galectin 3; D050505:Mutant Proteins
keywords: collagen; galectin-3; glycome; lipopolysaccharide