title: Proteomic identification of autoantibodies in sera from patients with ovarian cancer as possible diagnostic biomarkers.
abstract: OBJECTIVE
  Accumulating evidence shows that various types of cancers induce a specific immune response resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from patients with ovarian cancer, especially clear cell carcinoma (CCC), as novel diagnostic markers for the disease.
  
  
  METHODS
  The reactivity of individual sera from patients was examined by two-dimensional (2-D) immunoblotting using lysates of CCC cell lines, ES-2 and RMG-1, as antigens to identify autoantigens. ELISA was established to quantitatively measure autoantibody titer of patients' sera.
  
  
  RESULTS
  Autoantibodies against RhoGDI were induced in sera of ovarian cancer patients. Elevated levels of autoantibodies against heterogeneous nuclear ribonucleoprotein L (hnRNPL) and a mitochondrial protein, dihydrolipoamide dehydrogenase (DLD), were detected in patients with CCC.
  
  
  CONCLUSIONS
  Autoantibodies against RhoGDI and hnRNPL and DLD may serve as novel diagnostic markers for ovarian cancer and CCC, respectively.
authors: Department of Obstetrics and Gynecology, Nippon Medical School Main Hospital, Tokyo, Japan.
authors: Koichi
authors: K
authors: Yoneyama
authors: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors: Shigeyuki
authors: S
authors: Kojima
authors: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors: Yoshiko
authors: Y
authors: Kodani
authors: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors: Nozomi
authors: N
authors: Yamaguchi
authors: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors: Akira
authors: A
authors: Igarashi
authors: Department of Obstetrics and Gynecology, Nippon Medical School Main Hospital, Tokyo, Japan.
authors: Keisuke
authors: K
authors: Kurose
authors: Department of Obstetrics and Gynecology, Nippon Medical School Main Hospital, Tokyo, Japan.
authors: Rieko
authors: R
authors: Kawase
authors: Department of Obstetrics and Gynecology, Nippon Medical School Main Hospital, Tokyo, Japan.
authors: Toshiyuki
authors: T
authors: Takeshita
authors: Division of Cellular Proteomics, Institute of Medical Science, The University of Tokyo, Tokyo, Japan Division of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan hattoris@pharm.kitasato-u.ac.jp.
authors: Seisuke
authors: S
authors: Hattori
authors: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors: Kinya
authors: K
authors: Nagata
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood*; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology*; D040901:Proteomics
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor
keywords: Autoantibody; biomarker; diagnosis; ovarian cancer slot-blot