title: Isolation of New Delhi metallo-β-lactamase 1-producing Vibrio cholerae non-O1, non-O139 strain carrying ctxA, st and hly genes in southern Vietnam.
abstract: Vibrio cholerae non-O1, non-O139 (VC_NAG) organisms are universally present in the aquatic environment and regarded as non-pathogenic bacteria. However, considering that they do occasionally induce gastroenteritis, a study of their virulence and antibiotic resistance genes is important. The presence of enteropathogenic genes, including ctxA, VC_NAG-specific heat-stable toxin gene (st), hemolysin (hly), and zona occludens toxin (zot) was determined by PCR in 100 VC_NAG strains isolated in southern Vietnam in 2010-2013 from 94 environmental and six human origins. These 100 VC_NAG strains were also tested phenotypically and genotypically for the presence of the New Delhi metallo-β-lactamase (NDM-1). Of the 100 VC_NAG strains tested, six were positive for ctxA; five from the environment and one of human origin. The st gene was detected in 17 isolates, 15 and two of which were of environmental and human origins, respectively. Gene hly was detected in 19 VC_NAG strains examined, two of which were isolated from humans and 17 from environments. The zot gene was not detected in any of the strains tested. Three VC_NAG strains of environmental origin were confirmed to produce NDM-1 and the blaNDM-1 gene was detected in those strains by PCR. Of note, one of the three NDM-1-producing VC_NAG strains was confirmed to carry ctxA, st and hly genes concurrently. This is the first report of isolation of NDM-1-producing VC_NAG strains in Vietnam.
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Tai The
authors: TT
authors: Diep
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Nhi Thi Ngoc
authors: NT
authors: Nguyen
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Thi Ngoc Cat
authors: TN
authors: Nguyen
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Huy Khac
authors: HK
authors: An
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Truong Quang
authors: TQ
authors: Nguyen
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Vu Hoang
authors: VH
authors: Nguyen
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Thuong Van
authors: TV
authors: Nguyen
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Thu Ngoc Anh
authors: TN
authors: Nguyen
authors: Department of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama Shinjuku, Tokyo, 162-8640, Japan.
authors: Hidemasa
authors: H
authors: Izumiya
authors: Department of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama Shinjuku, Tokyo, 162-8640, Japan.
authors: Makoto
authors: M
authors: Ohnishi
authors: Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki City, Nagasaki, 852-8523, Japan.|Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University, c/o National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hanoi, Vietnam.
authors: Tetsu
authors: T
authors: Yamashiro
authors: Laboratory of Bacteria Microbiology and Immunology Department, Pasteur Institute of Ho Chi Minh City, 167 Pasteur Street District 3, Ho Chi Minh City, Vietnam.
authors: Lan Thi Phuong
authors: LT
authors: Nguyen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002771:Cholera / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins; D004768:Enterotoxins / Q000235:genetics*; D004783:Environmental Microbiology; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D044163:Vibrio cholerae non-O1 / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D014744:Vietnam; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004731:Endotoxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; C084287:hlyA protein, Vibrio cholerae; C037420:stN protein, Vibrio cholerae; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: NAG vibrio; Vibrio cholerae; blaNDM-1; virulent gene