title: [Analysis of FABP4 expression pattern in rump fat deposition and metabolism of Altay sheep].
abstract: FABP4 (Fatty acid binding protein 4) is a hot candidate gene in fat deposition and lipid metabolism and participates in the transport and metabolism of intracellular free fatty acids. We aim to study the role of FABP4 in fat deposition and metabolism of the rump fat in Altay sheep. In this study, bioinformatics method was used to analyze the protein sequence homology among 10 species, and RT-PCR was employed to detect FABP4 tissue profiling of Altay sheep. An animal model simulating the rump fat deposition and metabolism of Altay sheep was established by continuous starvation, and qPCR and iTRAQ (isobaric tags for relative and absolute quantitation) were used to detecte FABP4 mRNA and protein expression changes in the control and continuous starvation groups, respectively. Sequence analysis showed that FABP4 protein sequence is highly conserved among species, suggesting an important biological function during evolution for FABP4. The RT-PCR result confirmed that FABP4 mRNA was highly expressed in intestinal and rump fat, suggesting that FABP4 plays an important physiological role in fat tissues. We did not find significant differences in FABP4 mRNA and protein between control and continuous starvation groups (P>0.05), which indicates that FABP4 may not be the key gene in fat deposition and metabolism in Altay sheep.The results above lay a foundation for further studies of FABP4 in rump or tail fat.
authors: 1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Rui-Xia
authors: RX
authors: Xu
authors: Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Lei
authors: L
authors: Gao
authors: 1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Wei-Li
authors: WL
authors: Zhao
authors: 1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Wei
authors: W
authors: Zhang
authors: 1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Guang-Chao
authors: GC
authors: Song
authors: Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Shang-Quan
authors: SQ
authors: Gan
authors: 1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.|2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
authors: Guo-Qing
authors: GQ
authors: Shi
mesh_terms: D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005260:Female; D050356:Lipid Metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D012756:Sheep
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger