title: [Changes in chemokine receptor 4, interleukin-6, and collagen X expression in the ATDC5 cell line stimulated by cyclic tensile strain and stromal cell-derived factor-1].
abstract: OBJECTIVE
  This study further explores the stromal cell-derived factor-1 (SDF-1)/chemokine receptor 4 (CXCR4) signaling axis mechanism in temporomandibular joint osteoarthritis (OA) by detecting the changes in CXCR4, interleukin (IL)-6, and collagen X expression in the ATDC5 cell line stimulated by the cyclic tensile strain and SDF-1.
  
  
  METHODS
  Insulin-transferrin-selenium (ITS) was used to induce ATDC5 cells to differentiate into chondrocyte-like cells. After three weeks, the cells were divided into two groups: those with and without cyclic tensile strain. These groups were further divided into the negative control and SDF-1 groups. Strain force of 20% was applied. After 12 h, the total proteins were extracted from cells of the four groups, and Western blot analysis was used to detect the changes in CXCR4, IL-6, and collagen X expression.
  
  
  RESULTS
  SDF-1 could enhance CXCR4, IL-6, and collagen X expressions in the chondrocytes, and 20% tensile strain force could further upregulate the three factors.
  
  
  CONCLUSIONS
  Under abnormal tensile force, SDF-1 can upregulate its specific receptor CXCR4, thus increasing its-binding efficiency and resulting in the activation of the SDF-1/CXCR4 axis. This condition enhances the expressions of IL-6 and other inflammatory factors and directly damages to cartilage tissue. Such damage directly promotes chondrocyte hypertrophy, which enhances collagen X expression.
authors: Kuang
authors: K
authors: Bin
authors: Wang
authors: W
authors: Qingyu
authors: Song
authors: S
authors: Rong
authors: Sun
authors: S
authors: Yanyan
authors: Chai
authors: C
authors: Zhiguo
authors: Duan
authors: D
authors: Yinzhong
authors: Dai
authors: D
authors: Juan
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D054377:Chemokine CXCL12; D003094:Collagen; D006801:Humans; D015850:Interleukin-6; D019718:Receptors, CXCR4; D015398:Signal Transduction; D017154:Stromal Cells
chemical_list: D054377:Chemokine CXCL12; D015850:Interleukin-6; D019718:Receptors, CXCR4; D003094:Collagen