title: In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines.
abstract: One of the promising strategies for improvement of cancer treatment is based on magnetic drug delivery systems, thus avoiding side effects of standard chemotherapies. Superparamagnetic iron oxide (SPIO) nanoparticles have ideal properties to become a targeted magnetic drug delivery contrast probes, named theranostics. We worked with SPIO condensed colloidal nanocrystal clusters (MagAlg) prepared through a new soft biomineralization route in the presence of alginate as the polymeric shell and loaded with doxorubicin (DOX). The aim of this work was to study the in vitro cytotoxicity of these new MagAlg-DOX systems on mouse fibroblast and breast carcinoma cell lines. For proper analysis and understanding of cell behavior after administration of MagAlg-DOX compared with free DOX, a complex set of in vitro tests, including production of reactive oxygen species, comet assay, cell cycle determination, gene expression, and cellular uptake, were utilized. It was found that the cytotoxic effect of MagAlg-DOX system is delayed compared to free DOX in both cell lines. This was attributed to the different mechanism of internalization of DOX and MagAlg-DOX into the cells, together with the fact that the drug is strongly bound on the drug nanocarriers. We discovered that nanoparticles can attenuate or even inhibit the effect of DOX, particularly in the tumor MCF7 cell line. This is a first comprehensive study on the cytotoxic effect of DOX-loaded SPIO compared with free DOX on healthy and cancer cell lines, as well as on the induced changes in gene expression.
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Katerina
authors: K
authors: Tomankova
authors: Regional Centre of Advanced Technologies and Materials, Departments of Physical Chemistry and Experimental Physics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors: Katerina
authors: K
authors: Polakova
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Klara
authors: K
authors: Pizova
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Svatopluk
authors: S
authors: Binder
authors: Regional Centre of Advanced Technologies and Materials, Departments of Physical Chemistry and Experimental Physics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors: Marketa
authors: M
authors: Havrdova
authors: Regional Centre of Advanced Technologies and Materials, Departments of Physical Chemistry and Experimental Physics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors: Mary
authors: M
authors: Kolarova
authors: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Eva
authors: E
authors: Kriegova
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Jana
authors: J
authors: Zapletalova
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Lukas
authors: L
authors: Malina
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Jana
authors: J
authors: Horakova
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Jakub
authors: J
authors: Malohlava
authors: Department of Materials Science, University of Patras, Patras, Greece.
authors: Argiris
authors: A
authors: Kolokithas-Ntoukas
authors: Department of Materials Science, University of Patras, Patras, Greece.
authors: Aristides
authors: A
authors: Bakandritsos
authors: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors: Hana
authors: H
authors: Kolarova
authors: Regional Centre of Advanced Technologies and Materials, Departments of Physical Chemistry and Experimental Physics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors: Radek
authors: R
authors: Zboril
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects*; D003102:Colloids / Q000737:chemistry / Q000633:toxicity; D004317:Doxorubicin / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D000970:Antineoplastic Agents; D003102:Colloids; D058185:Magnetite Nanoparticles; D004317:Doxorubicin
keywords: DOX/SPIO nanocarriers; doxorubicin; in vitro cytotoxicity; superparamagnetic iron oxide nanoparticles