title: Genotyping coronaviruses associated with feline infectious peritonitis.
abstract: Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5 % of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young cats. In this study, we report the complete coding genome sequences of six FCoVs: three from faecal samples from healthy cats and three from tissue lesion samples from cats with confirmed FIP. The six samples were obtained over a period of 8 weeks at a single-site cat rescue and rehoming centre in the UK. We found amino acid differences located at 44 positions across an alignment of the six virus translatomes and, at 21 of these positions, the differences fully or partially discriminated between the genomes derived from the faecal samples and the genomes derived from the tissue lesion samples. In this study, two amino acid differences fully discriminated the two classes of genomes: these were both located in the S2 domain of the virus surface glycoprotein gene. We also identified deletions in the 3c protein ORF of genomes from two of the FIP samples. Our results support previous studies that implicate S protein mutations in the pathogenesis of FIP.
authors: School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK.
authors: Catherine S
authors: CS
authors: Lewis
authors: School of Veterinary Sciences, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors: Emily
authors: E
authors: Porter
authors: School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK.
authors: David
authors: D
authors: Matthews
authors: Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurer Strasse 268, 8057 Zurich, Switzerland.
authors: Anja
authors: A
authors: Kipar
authors: School of Veterinary Sciences, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors: Séverine
authors: S
authors: Tasker
authors: School of Veterinary Sciences, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors: Christopher R
authors: CR
authors: Helps
authors: School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK.
authors: Stuart G
authors: SG
authors: Siddell
mesh_terms: D000818:Animals; D002415:Cats; D016000:Cluster Analysis; D016765:Coronavirus, Feline / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016766:Feline Infectious Peritonitis / Q000821:virology*; D023061:Gene Order; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D006113:United Kingdom
chemical_list: D012367:RNA, Viral