title: High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.
abstract: Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
authors: Andre C
authors: AC
authors: Stiel
authors: Xosé Luís
authors: XL
authors: Deán-Ben
authors: Yuanyuan
authors: Y
authors: Jiang
authors: Vasilis
authors: V
authors: Ntziachristos
authors: Daniel
authors: D
authors: Razansky
authors: Gil G
authors: GG
authors: Westmeyer
mesh_terms: D021621:Imaging, Three-Dimensional; D008164:Luminescent Proteins; D061088:Photoacoustic Techniques / Q000379:methods*; D059629:Signal-To-Noise Ratio; D014054:Tomography / Q000379:methods*
chemical_list: D008164:Luminescent Proteins