title: Neutron reflectometry from poly (ethylene-glycol) brushes binding anti-PEG antibodies: evidence of ternary adsorption.
abstract: Neutron reflectometry provides evidence of ternary protein adsorption within polyethylene glycol (PEG) brushes. Anti-PEG Immunoglobulin G antibodies (Abs) binding the methoxy terminated PEG chain segment specifically adsorb onto PEG brushes grafted to lipid monolayers on a solid support. The Abs adsorb at the outer edge of the brush. The thickness and density of the adsorbed Ab layer, as well as its distance from the grafting surface grow with increasing brush density. At high densities most of the protein is excluded from the brush. The results are consistent with an inverted "Y" configuration with the two FAB segments facing the brush. They suggest that increasing the grafting density favors narrowing of the angle between the FAB segments as well as overall orientation of the bound Abs perpendicular to the surface.
authors: Institut Laue-Langevin, 71 av. Des Martyrs, BP 156, 38000 Grenoble, France; Biomaterials Department, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany. Electronic address: schneck@mpikg.mpg.de.
authors: Emanuel
authors: E
authors: Schneck
authors: Institut Laue-Langevin, 71 av. Des Martyrs, BP 156, 38000 Grenoble, France; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany.
authors: Ida
authors: I
authors: Berts
authors: Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier Grenoble, CNRS UMR 5588, BP87, 38042 Saint-Martin d'Hères, France. Electronic address: avraham.halperin@ujf-grenoble.fr.
authors: Avraham
authors: A
authors: Halperin
authors: Synchrotron Soleil, L'Orme des Merisiers, Saint-Aubin - BP 48, 91192 Gif-sur-Yvette Cedex, France.
authors: Jean
authors: J
authors: Daillant
authors: Institut Laue-Langevin, 71 av. Des Martyrs, BP 156, 38000 Grenoble, France. Electronic address: fragneto@ill.eu.
authors: Giovanna
authors: G
authors: Fragneto
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000378:metabolism*; D009502:Neutrons; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D011092:Polyethylene Glycols
keywords: Biocompatibility; Immune response; Langmuir Blodgett film; Polyethylene oxide; Protein adsorption