title: Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats.
abstract: Glucagon-like peptide-1 (GLP-1), which is produced and released from enteroendocrine L cells, plays pivotal roles in postprandial glycaemia. The ingestion of resistant maltodextrin (RMD), a water-soluble non-digestible saccharide, improves the glycaemic response. In the present study, we examined whether the continuous feeding of RMD to rats affected GLP-1 levels and glycaemic control. Male Sprague-Dawley rats (6 weeks of age) were fed an American Institute of Nutrition (AIN)-93G-based diet containing either cellulose (5 %) as a control, RMD (2.5 or 5 %), or fructo-oligosaccharides (FOS, 2.5 or 5 %) for 7 weeks. During the test period, an intraperitoneal glucose tolerance test (IPGTT) was performed after 6 weeks. Fasting GLP-1 levels were significantly higher in the 5 % RMD group than in the control group after 6 weeks. The IPGTT results showed that the glycaemic response was lower in the 5 % RMD group than in the control group. Lower caecal pH, higher caecal tissue and content weights were observed in the RMD and FOS groups. Proglucagon mRNA levels were increased in the caecum and colon of both RMD and FOS groups, whereas caecal GLP-1 content was increased in the 5 % RMD group. In addition, a 1 h RMD exposure induced GLP-1 secretion in an enteroendocrine L-cell model, and single oral administration of RMD increased plasma GLP-1 levels in conscious rats. The present study demonstrates that continuous ingestion of RMD increased GLP-1 secretion and production in normal rats, which could be stimulated by its direct and indirect (enhanced gut fermentation) effects on GLP-1-producing cells, and contribute to improving glucose tolerance.
authors: Laboratory of Nutritional Biochemistry, Research Faculty of Agriculture, Hokkaido University,Kita-9, Nishi-9, Kita-ku,Sapporo060-8589,Japan.
authors: Tohru
authors: T
authors: Hira
authors: Graduate School of Agriculture, Hokkaido University,Sapporo,Japan.
authors: Asuka
authors: A
authors: Ikee
authors: Research and Development, Matsutani Chemical Industry Company Limited,Itami, Hyogo,Japan.
authors: Yuka
authors: Y
authors: Kishimoto
authors: Research and Development, Matsutani Chemical Industry Company Limited,Itami, Hyogo,Japan.
authors: Sumiko
authors: S
authors: Kanahori
authors: Laboratory of Nutritional Biochemistry, Research Faculty of Agriculture, Hokkaido University,Kita-9, Nishi-9, Kita-ku,Sapporo060-8589,Japan.
authors: Hiroshi
authors: H
authors: Hara
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects*; D002432:Cecum / Q000737:chemistry / Q000378:metabolism; D003106:Colon / Q000737:chemistry / Q000378:metabolism; D004032:Diet; D004063:Digestion; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000378:metabolism; D005215:Fasting / Q000502:physiology*; D005285:Fermentation / Q000187:drug effects; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006863:Hydrogen-Ion Concentration; D008297:Male; D011134:Polysaccharides / Q000008:administration & dosage* / Q000378:metabolism; D052336:Proglucagon / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D011134:Polysaccharides; D012333:RNA, Messenger; D052336:Proglucagon; C008315:maltodextrin; D052216:Glucagon-Like Peptide 1
keywords: Fructo-oligosaccharides; Glucagon-like peptide-1; Glucose tolerance; Proglucagon; Resistant maltodextrin