title: Protective effect of panax notoginseng saponins on acute ethanol-induced liver injury is associated with ameliorating hepatic lipid accumulation and reducing ethanol-mediated oxidative stress.
abstract: The aim of present study was to evaluate the effects of Panax notoginseng saponins (PNS) against acute ethanol-induced liver injury and further to elucidate its probable mechanisms. Mice were treated with PNS (100 or 300 mg/kg) once daily for seven consecutive days priors to ethanol gavage (4.7 g/kg) every 12 h for a total of three doses. Acute alcohol gavage dramatically significantly increased serum activities of alanine aminotransferase (ALT) (23.4 ± 5.0 IU/L vs 11.7 ± 4.1 IU/L) and aspartate aminotransferase (AST) (52.6 ± 14.9 IU/L vs 31.1 ± 12.9 IU/L), and hepatic triglyceride level (4.04 ± 0.64 mg/g vs 1.92 ± 0.34 mg/g), these elevations were significantly diminished by pretreatment with PNS at dose of 100 mg/kg or 300 mg/kg. Alcohol exposure markedly induced the lipolysis of white adipose tissue (WAT), up-regulated protein expression of the phosphorylated hormone-sensitive lipase (p-HSL, p < 0.01), and total HSL (p < 0.01), and enhanced fatty acid uptake capacity in liver as indicated by increasing hepatic CD36 expression (p < 0.01), these effects were attenuated by PNS treatment. Additionally, PNS suppressed the elevation of reactive oxygen species (ROS) production and malondialdehyde (MDA) content, reduced TNF-α and IL-6 levels, restored glutathione (GSH) level, enhanced the superoxide dismutase (SOD) activity in liver, and abrogated cytochrome P450 2E1 (CYP2E1) induction. These data demonstrated that pretreatment with PNS protected against acute ethanol-induced liver injury, possibly through ameliorating hepatic lipid accumulation and reducing CYP2E1-mediated oxidative stress. Our findings also suggested that PNS may be potential to be developed as an effective agent for acute ethanol-induced liver injury.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macao, P. R. China.
authors: Ren-Bo
authors: RB
authors: Ding
authors: Ke
authors: K
authors: Tian
authors: Yi-Wei
authors: YW
authors: Cao
authors: Jiao-Lin
authors: JL
authors: Bao
authors: Meng
authors: M
authors: Wang
authors: Chengwei
authors: C
authors: He
authors: Yuanjia
authors: Y
authors: Hu
authors: Huanxing
authors: H
authors: Su
authors: Jian-Bo
authors: JB
authors: Wan
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D000431:Ethanol / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D053723:Panax notoginseng / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000008:administration & dosage*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; D012503:Saponins; D014280:Triglycerides; D000431:Ethanol; D008315:Malondialdehyde; D005978:Glutathione
keywords: Panax notoginseng saponins; acute ethanol-induced liver injury; hepatic lipid accumulation; lipolysis; oxidative stress