title: Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro.
abstract: The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China; Stomatologic Hospital & College, Anhui Medical University, Key Lab of Oral Diseases Research of Anhui Province, Hefei, China.
authors: Jing
authors: J
authors: Zhang
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China.
authors: Zhihua
authors: Z
authors: Wang
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China.
authors: Yong
authors: Y
authors: Jiang
authors: Treatment center of oral diseases, The 306th Hospital of People׳s Liberation Army, Beijing, China.
authors: Zhongying
authors: Z
authors: Niu
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China.
authors: Lei
authors: L
authors: Fu
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China.
authors: Zhirong
authors: Z
authors: Luo
authors: Oral Biology, School of Dentistry, University of Birmingham, B4 6NN, UK.
authors: Paul R
authors: PR
authors: Cooper
authors: Oral Biology, School of Dentistry, University of Birmingham, B4 6NN, UK.
authors: Anthony J
authors: AJ
authors: Smith
authors: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi׳an, China. Electronic address: hewenxi@fmmu.edu.cn.
authors: Wenxi
authors: W
authors: He
mesh_terms: D000293:Adolescent; D053687:Adult Stem Cells / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology*; D006801:Humans; D051839:NFI Transcription Factors / Q000502:physiology*; D014074:Tooth Calcification
chemical_list: D051839:NFI Transcription Factors; C497647:NFIC protein, human; D000469:Alkaline Phosphatase
keywords: Differentiation; Nuclear factor; Proliferation; SCAPs