title: Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring.
abstract: Early nicotine exposure causes future obesity and insulin resistance. We evaluated the long-term effect of the maternal nicotine exposure during lactation in liver oxidative status, insulin sensitivity and morphology in adult offspring. Two days after birth, osmotic minipumps were implanted in the dams: nicotine (N), 6 mg/kg/day for 14 days or saline (C). Offspring were killed at 180 days. Protein content of superoxide dismutase, glutathione peroxidase, catalase, nitrotyrosine, 4HNE, IRS1, Akt1 and PPARs were measured. MDA, bound protein carbonyl content, SOD, GPx and catalase activities were determined in liver and plasma. Hepatic morphology and triglycerides content were evaluated. Albumin and bilirubin were determined. In plasma, N offspring had higher catalase activity, and SOD/GPx ratio, albumin and bilirubin levels but lower MDA content. In liver, they presented higher MDA and 4HNE levels, bound protein carbonyl content, SOD activity but lower GPx activity. N offspring presented an increase of lipid droplet, higher triglyceride content and a trend to lower PPARα in liver despite unchanged insulin signaling pathway. Early nicotine exposure causes oxidative stress in liver at adulthood, while protect against oxidative stress at plasma level. In addition, N offspring develop liver microsteatosis, which is related to oxidative stress but not to insulin resistance.
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: E P
authors: EP
authors: Conceição
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: N
authors: N
authors: Peixoto-Silva
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: C R
authors: CR
authors: Pinheiro
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: E
authors: E
authors: Oliveira
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: E G
authors: EG
authors: Moura
authors: Laboratory of Endocrine Physiology, Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: pclisboa.uerj@yahoo.com.br.
authors: P C
authors: PC
authors: Lisboa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D001663:Bilirubin / Q000097:blood; D002374:Catalase / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005234:Fatty Liver / Q000139:chemically induced / Q000473:pathology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000097:blood; D007333:Insulin Resistance; D007774:Lactation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D009538:Nicotine / Q000009:adverse effects; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation; D051057:Proto-Oncogene Proteins c-akt / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000097:blood; D014280:Triglycerides / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D000975:Antioxidants; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D012709:Serum Albumin; D014280:Triglycerides; C002744:3-nitrotyrosine; D014443:Tyrosine; D009538:Nicotine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C494920:Akt1 protein, rat; D051057:Proto-Oncogene Proteins c-akt; D001663:Bilirubin
keywords: Insulin; Liver steatosis; Nicotine; Oxidative stress