title: Electrochemical pretreatment of amino-carbon nanotubes on graphene support as a novel platform for bilirubin oxidase with improved bioelectrocatalytic activity towards oxygen reduction.
abstract: The electrochemical conditioning of amino-carbon nanotubes (CNTs) on a graphene support in an alkaline solution is used to produce -NHOH as hydrophilic functional groups for the efficient immobilization of bilirubin oxidase enzyme. The application of the immobilized enzyme for the direct electrocatalytic reduction of O2 is investigated. The onset potential of 0.81 V versus NHE and peak current density of 2.3 mA cm(-2) for rotating modified electrode at 1250 rpm, indicate improved biocatalytic activity of the proposed system for O2 reduction.
authors: Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj (Iran).
authors: Aso
authors: A
authors: Navaee
authors: Abdollah
authors: A
authors: Salimi
authors: Fereydoon
authors: F
authors: Jafari
mesh_terms: D055162:Biocatalysis; D004563:Electrochemistry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry*; D010100:Oxygen
chemical_list: D037742:Nanotubes, Carbon; D006108:Graphite; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D010100:Oxygen
keywords: amino-carbon nanotubes; bilirubin oxidase; electrochemical conditioning; enzyme immobilization; graphene