title: Structural features of a novel polysaccharide isolated from a New Zealand Maori mushroom Iliodiction cibarium.
abstract: A purified water-soluble fraction (ICP5) of a polysaccharide, isolated from a local Maori mushroom Iliodiction cibarium in New Zealand, was investigated for its structural properties. Size exclusion chromatography and dynamic light scattering showed that ICP5 had a large MW of 1.6 × 10(5) Da with a hydrodynamic diameter of 83 ± 8 nm. Particle size measurements also displayed the tendency of ICP5 to aggregate when suspended in water. The results of GC-MS, FTIR and NMR analyses allowed some characteristics of the chemical structure of ICP5 to be determined. GC-MS results showed that ICP5 contained only glucose (81.61%), galactose (12.90%) and mannose (5.49%) monomers. The characterized fragment structures of ICP5 were found to be dominantly consisting of uronic acids, which formed a backbone containing 1,4-β-D-GlcpA. A small amount of unsaturated uronic acid also appeared to be present.
authors: School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. Electronic address: lren022@aucklanduni.ac.nz.
authors: Lu
authors: L
authors: Ren
authors: Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand. Electronic address: P.J.Edwards@massey.ac.nz.
authors: Patrick J B
authors: PJ
authors: Edwards
authors: School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors: Conrad O
authors: CO
authors: Perera
authors: School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors: Yacine
authors: Y
authors: Hemar
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002236:Carbohydrate Conformation; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D009520:New Zealand; D014574:Uronic Acids / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D062610:Fungal Polysaccharides; D014574:Uronic Acids
keywords: Aggregation; Molecular weight; Mushroom polysaccharide; NMR; Particle size; Structure