title: Chromobacterium amazonense sp. nov. isolated from water samples from the Rio Negro, Amazon, Brazil.
abstract: The taxonomic position of a bacterium isolated from water samples from the Rio Negro, in Amazon, Brazil, was determined by using a polyphasic approach. The organism formed a distinct phyletic line in the Chromobacterium 16S rRNA gene tree and had chemotaxonomic and morphological properties consistent with its classification in this genus. It was found to be closely related to Chromobacterium vaccinii DSM 25150(T) (98.6 % 16S rRNA gene similarity) and shared 98.5 % 16S rRNA gene similarity with Chromobacterium piscinae LGM 3947(T). DNA-DNA relatedness studies showed that isolate CBMAI 310(T) belongs to distinct genomic species. The isolate was readily distinguished from the type strain of these species using a combination of phenotypic and chemotaxonomic properties. Thus, based on genotypic and phenotypic data, it is proposed that isolate CBMAI 310(T) (=DSM 26508(T)) be classified in the genus Chromobacterium as the type strain of a novel species, namely, Chromobacterium amazonense sp. nov.
authors: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Avenida Alexandre Cazelatto, 999, Campinas, SP, CEP 13083-218, Brazil.
authors: Claudia Beatriz Afonso
authors: CB
authors: Menezes
authors: Mariana Ferreira
authors: MF
authors: Tonin
authors: Daniele Bussioli Alves
authors: DB
authors: Corrêa
authors: Márcia
authors: M
authors: Parma
authors: Itamar Soares
authors: IS
authors: de Melo
authors: Tiago Domingues
authors: TD
authors: Zucchi
authors: Suzete Aparecida Lanza
authors: SA
authors: Destéfano
authors: Fabiana
authors: F
authors: Fantinatti-Garboggini
mesh_terms: D015373:Bacterial Typing Techniques; D001938:Brazil; D002861:Chromobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S