title: Isoform composition and gene expression of thick and thin filament proteins in striated muscles of mice after 30-day space flight.
abstract: Changes in isoform composition, gene expression of titin and nebulin, and isoform composition of myosin heavy chains as well as changes in titin phosphorylation level in skeletal (m. gastrocnemius, m. tibialis anterior, and m. psoas) and cardiac muscles of mice were studied after a 30-day-long space flight onboard the Russian spacecraft "BION-M" number 1. A muscle fibre-type shift from slow-to-fast and a decrease in the content of titin and nebulin in the skeletal muscles of animals from "Flight" group was found. Using Pro-Q Diamond staining, an ~3-fold increase in the phosphorylation level of titin in m. gastrocnemius of mice from the "Flight" group was detected. The content of titin and its phosphorylation level in the cardiac muscle of mice from "Flight" and "Control" groups did not differ; nevertheless an increase (2.2 times) in titin gene expression in the myocardium of flight animals was found. The observed changes are discussed in the context of their role in the contractile activity of striated muscles of mice under conditions of weightlessness.
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia ; Pushchino State Institute of Natural Science, Nauki Street 3, Pushchino 142290, Russia.
authors: Anna
authors: A
authors: Ulanova
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia.
authors: Yulia
authors: Y
authors: Gritsyna
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia.
authors: Ivan
authors: I
authors: Vikhlyantsev
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia.
authors: Nikolay
authors: N
authors: Salmov
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia.
authors: Alexander
authors: A
authors: Bobylev
authors: Dagestan State University, Gadzhieva Street 43a, Makhachkala, Republic of Dagestan 367000, Russia.
authors: Zarema
authors: Z
authors: Abdusalamova
authors: Institute of Cell Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia.
authors: Vadim
authors: V
authors: Rogachevsky
authors: SRC, Institute for Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Street 76A, Moscow 123007, Russia.
authors: Boris
authors: B
authors: Shenkman
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino 142290, Russia ; Pushchino State Institute of Natural Science, Nauki Street 3, Pushchino 142290, Russia.
authors: Zoya
authors: Z
authors: Podlubnaya
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D064211:Connectin / Q000235:genetics / Q000378:metabolism; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D054792:Muscle, Striated / Q000378:metabolism* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D009929:Organ Size; D010766:Phosphorylation; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism / Q000648:ultrastructure; D013026:Space Flight
chemical_list: D064211:Connectin; D018841:HSP90 Heat-Shock Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; C035620:nebulin; D018995:Myosin Heavy Chains