title: A novel fluorescence reporter system for the characterization of dairy goat mammary epithelial cells.
abstract: Goat mammary epithelial cells (GMECs) are a useful model to understand the physiological function of mammary glands and to assess the efficiency of mammary-specific vectors. The aim of this study was to develop an effective and convenient way to evaluate the secretory capacity of GMECs in primary culture. In this study, we developed a reporter system using fluorescent proteins driven by the CSN2 (Capra hircus beta-casein) gene promoter to detect the secretory capacity of GMECs. Additionally, we evaluated the efficiency of the reporter system by determining the expression of cytoskeletal proteins and beta-casein protein. The results suggest that this reporter system provides an easy, convenient and effective method to detect the function of milk synthesis in GMECs. Primary cultures of GMECs were homogeneous and retained the function of milk synthesis, prompting their usefulness as a model for further studies.
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Lizhong
authors: L
authors: Wang
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Caifang
authors: C
authors: Ren
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jihao
authors: J
authors: You
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Yixuan
authors: Y
authors: Fan
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Yongjie
authors: Y
authors: Wan
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Yanli
authors: Y
authors: Zhang
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: caeet@njau.edu.cn.
authors: Feng
authors: F
authors: Wang
authors: Jiangsu Livestock Embryo Engineering Laboratory, Jiangsu Engineering Technology Research Center of Mutton Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: hmr@njau.edu.cn.
authors: Mingrui
authors: M
authors: Huang
mesh_terms: D000818:Animals; D002364:Caseins / Q000235:genetics*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D017930:Genes, Reporter; D006041:Goats / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007774:Lactation; D042361:Mammary Glands, Human / Q000166:cytology* / Q000502:physiology; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic
chemical_list: D002364:Caseins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Beta-casein promoter; EGFP; Goat; Mammary epithelial cells