title: Glargine Promotes Human Colorectal Cancer Cell Proliferation via Upregulation of miR-95.
abstract: Several studies have shown a correlation between glargine use and cancer risk. However, the role of glargine in carcinogenesis, especially in colorectal cancer (CRC), is still inconclusive. The aim of this study was to investigate the influence of glargine on proliferation of CRC cells and its possible mechanism. Effect of glargine on the cell proliferation was tested in HCT-116 and SW480 cells by MTT assay, and apoptosis was measured by flow cytometry. The expression of microRNA-95 (miR-95) and sorting nexin 1 (SNX1) protein was also determined by real-time PCR and Western blotting, respectively. The results showed that high dose glargine (from 150 to 300 nM) promoted proliferation and inhibit2ed apoptosis of CRC cells compared with untreated cells. Moreover, glargine could upregulate miR-95 and downregulate SNX1 protein expression in CRC cells. These data show that glargine may indeed trigger cellular proliferation in CRC, probably by regulating miR-95.
authors: Department of Radiation Oncology of Clinical Cancer Center, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors: J
authors: J
authors: Qin
authors: Department of Cadre Medicine, Division of Endocrinology and Metabolism, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors: J-A
authors: JA
authors: Teng
authors: Department of Gastrointestinal and Peripheral Vascular Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors: Z
authors: Z
authors: Zhu
authors: Department of Radiation Oncology of Clinical Cancer Center, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors: J-X
authors: JX
authors: Chen
authors: Department of Pharmacology, School of Pharmacy, Chengdu Medical College, Chengdu, China.
authors: Y-Y
authors: YY
authors: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D000069036:Insulin Glargine / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058305:Sorting Nexins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects* / Q000235:genetics
chemical_list: C575854:MIRN95 microRNA, human; D035683:MicroRNAs; C513978:SNX1 protein, human; D058305:Sorting Nexins; D000069036:Insulin Glargine