title: A microfluidic platform for high-throughput multiplexed protein quantitation.
abstract: We present a high-throughput microfluidic platform capable of quantitating up to 384 biomarkers in 4 distinct samples by immunoassay. The microfluidic device contains 384 unit cells, which can be individually programmed with pairs of capture and detection antibody. Samples are quantitated in each unit cell by four independent MITOMI detection areas, allowing four samples to be analyzed in parallel for a total of 1,536 assays per device. We show that the device can be pre-assembled and stored for weeks at elevated temperature and we performed proof-of-concept experiments simultaneously quantitating IL-6, IL-1β, TNF-α, PSA, and GFP. Finally, we show that the platform can be used to identify functional antibody combinations by screening 64 antibody combinations requiring up to 384 unique assays per device.
authors: Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
authors: Francesca
authors: F
authors: Volpetti
authors: Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
authors: Jose
authors: J
authors: Garcia-Cordero
authors: Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
authors: Sebastian J
authors: SJ
authors: Maerkl
mesh_terms: D015415:Biomarkers; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods / Q000592:standards; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D015203:Reproducibility of Results; D057188:Workflow
chemical_list: D015415:Biomarkers; D011506:Proteins