title: miR-155 promotes the growth of osteosarcoma in a HBP1-dependent mechanism.
abstract: Osteosarcoma (OS) is a type of malignant tumor arising from soft-tissues of bone and displays poor prognosis in most cases. However, the molecular mechanism by which OS initiates and progresses is still not completely elucidated. miR-155 has been shown to be overexpressed in OS specimen and cell lines. Our study is intended to explore the role of miR-155 in OS etiology. The data confirmed that miR-155 abundance is higher in OS samples than non-cancerous bone tissue. Inhibition of miR-155 suppressed the proliferation of OS cells and cell cycle progression in vitro, and the growth of OS xenografts in vivo. Wnt pathway was suppressed in OS cells by miR-155 inhibitors. HMG-box transcription factor 1 (HBP1), a strong Wnt pathway suppressor, was found to be a target of miR-155. Restoration of HBP1 abolished the effect of miR-155 on OS cells. Finally, miR-155 levels in OS tissues and serum are both inversely associated with the survival of OS patients. Collectively, miR-155 was identified to be among the list of OS-related oncogenic miRNAs, and HBP1-mediated Wnt signaling is involved with the role of miR-155 in OS progression.
authors: The Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University, 88 Jiankang Road, Weihui, 453100, China, Xiaohuisunhn@126.com.
authors: Xiaohui
authors: X
authors: Sun
authors: Xiaolin
authors: X
authors: Geng
authors: Jun
authors: J
authors: Zhang
authors: Hongxing
authors: H
authors: Zhao
authors: Ying
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D012097:Repressor Proteins / Q000378:metabolism*; D016019:Survival Analysis; D060449:Wnt Signaling Pathway
chemical_list: C486328:HBP1 protein, human; D006609:High Mobility Group Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins