title: Effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar without stress but different with mechanical compressive stress.
abstract: BACKGROUND
  This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress.
  
  
  METHODS
  Human recombinant TGF-β1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL) and Alizarin Red-S staining were conducted. Mechanical compressive stress was obtained by increasing the pressure of gaseous phase. OPG/RANKL expression was detected in both cells through quantitative real-time PCR.
  
  
  RESULTS
  Similar significant differences (P < 0.05) existed in OPG/RANKL change with increasing concentration of TGF-β1 without mechanical stress for cementoblasts and osteoblasts. However, under 3 h stress, OPG increased and RANKL decreased significantly (P < 0.01) but with similar OPG/RANKL change. Moreover, under 24 h stress, OPG change exhibited no difference (P > 0.05), but RANKL decreased significantly (P < 0.01) at 10 and 100 ng/mL TGF-β1 in cementoblasts. In osteoblasts, OPG increased significantly (P < 0.01) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference (P < 0.05) at 1 and 10 ng/mL.
  
  
  CONCLUSIONS
  The effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress. However, these effects are different under mechanical compressive stress.
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Xianrui
authors: X
authors: Yang
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Yanmin
authors: Y
authors: Wang
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Xianglong
authors: X
authors: Han
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Rui
authors: R
authors: Shu
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Tian
authors: T
authors: Chen
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Huan
authors: H
authors: Zeng
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Xin
authors: X
authors: Xu
authors: Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China.
authors: Lan
authors: L
authors: Huang
authors: Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China.
authors: Aishu
authors: A
authors: Ren
authors: Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China.
authors: Jinlin
authors: J
authors: Song
authors: Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China.
authors: Li
authors: L
authors: Cao
authors: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Ding
authors: D
authors: Bai
mesh_terms: D000818:Animals; D002460:Cell Line; D003739:Dental Cementum / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053244:Osteoprotegerin / Q000096:biosynthesis / Q000187:drug effects*; D053245:RANK Ligand / Q000096:biosynthesis / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D053773:Transforming Growth Factor beta1