title: Carboxylation of multiwalled carbon nanotube attenuated the cytotoxicity by limiting the oxidative stress initiated cell membrane integrity damage, cell cycle arrestment, and death receptor mediated apoptotic pathway.
abstract: In this study, the effects of carboxylated multiwalled carbon nanotubes (MWCNTs-COOH) on human normal liver cell line L02 was compared with that of pristine multiwalled carbon nanotubes (p-MWCNTs). It was shown that compared with MWCNTs-COOH, p-MWCNTs induced apoptosis, reduced the level of intracellular antioxidant glutathione more significantly, and caused severer cell membrane damage as demonstrated by lactate dehydrogenase leakage. Cell cycles were arrested by both MWCNTs, while p-MWCNTs induced higher ratio of G0/G1 phase arrestment as compared with MWCNTs-COOH. Caspase-8 was also activated after both MWCNTs exposure, indicating extrinsic apoptotic pathway was involved in the apoptosis induced by MWCNTs exposure, more importantly, MWCNTs-COOH significantly reduced the activation of caspase-8 as compared with p-MWCNTs. All these results suggested that MWCNTs-COOH might be safer for in vivo application as compared with p-MWCNTs.
authors: Department of Pharmaceutics, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan Province, 410013, People's Republic of China.
authors: Zhenbao
authors: Z
authors: Liu
authors: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan Province, 410083, People's Republic of China.
authors: Yanfei
authors: Y
authors: Liu
authors: Department of Medicinal Chemistry, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan Province, 410208, People's Republic of China.
authors: Dongming
authors: D
authors: Peng
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002264:Carboxylic Acids / Q000737:chemistry*; D002453:Cell Cycle; D002460:Cell Line; D002462:Cell Membrane; D006801:Humans; D037742:Nanotubes, Carbon; D018384:Oxidative Stress; D053218:Receptors, Death Domain / Q000502:physiology*
chemical_list: D002264:Carboxylic Acids; D037742:Nanotubes, Carbon; D053218:Receptors, Death Domain
keywords: cell cycle death receptor; cell membrane integrity; multiwalled carbon nanotubes; oxidative stress