title: Impact of exposure to low concentrations of nitric oxide on protein profile in murine and human pancreatic islet cells.
abstract: Homeostatic levels of nitric oxide (NO) protect efficiently against apoptotic death in both human and rodent pancreatic β cells, but the protein profile of this action remains to be determined. We have applied a 2 dimensional LC-MS-MALDI-TOF/TOF-based analysis to study the impact of protective NO in rat insulin-producing RINm5F cell line and in mouse and human pancreatic islets (HPI) exposed to serum deprivation condition. 24 proteins in RINm5F and 22 in HPI were identified to undergo changes in at least one experimental condition. These include stress response mitochondrial proteins (UQCRC2, VDAC1, ATP5C1, ATP5A1) in RINm5F cells and stress response endoplasmic reticulum proteins (HSPA5, PDIA6, VCP, GANAB) in HPI. In addition, metabolic and structural proteins, oxidoreductases and chaperones related with protein metabolism are also regulated by NO treatment. Network analysis of differentially expressed proteins shows their interaction in glucocorticoid receptor and NRF2-mediated oxidative stress response pathways and eNOS signaling. The results indicate that exposure to exogenous NO counteracts the impact of serum deprivation on pancreatic β cell proteome. Species differences in the proteins involved are apparent.
authors: a Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER)- Pablo de Olavide University; Biomedical Research Network (CIBER) of Diabetes and Related Metabolic Diseases; RED-TERCEL ; Seville , Spain.
authors: Rafael
authors: R
authors: Tapia-Limonchi
authors: Irene
authors: I
authors: Díaz
authors: Gladys M
authors: GM
authors: Cahuana
authors: Mario
authors: M
authors: Bautista
authors: Franz
authors: F
authors: Martín
authors: Bernat
authors: B
authors: Soria
authors: Juan R
authors: JR
authors: Tejedo
authors: Francisco J
authors: FJ
authors: Bedoya
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D018797:Cell Cycle Proteins / Q000032:analysis; D002460:Cell Line; D014450:Electron Transport Complex III / Q000032:analysis; D000091342:Endoplasmic Reticulum Chaperone BiP; D015870:Gene Expression / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D050417:Insulin-Secreting Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D007515:Islets of Langerhans / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D019704:Protein Disulfide-Isomerases / Q000032:analysis; D040901:Proteomics; D051381:Rats; D000074405:Valosin Containing Protein; D050995:Voltage-Dependent Anion Channel 1 / Q000032:analysis
chemical_list: D018797:Cell Cycle Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; C000588768:UQCRC2 protein, mouse; D009569:Nitric Oxide; D050995:Voltage-Dependent Anion Channel 1; D000251:Adenosine Triphosphatases; C000614178:VCP protein, human; D000074405:Valosin Containing Protein; C000614179:Vcp protein, mouse; C000614180:Vcp protein, rat; C496931:PDIA6 protein, human; D019704:Protein Disulfide-Isomerases; D014450:Electron Transport Complex III
keywords: islet survival; nitric oxide; pancreatic β cell; protein profile