title: Facile preparation of biocompatible and robust fluorescent polymeric nanoparticles via PEGylation and cross-linking.
abstract: Novel cross-linked copolymers of PEG-IM-PhNH2 are successfully synthesized through PEGylation via radical polymerization of 2-isocyanatoethyl methacrylate and poly(ethylene glycol) monomethyl ether methacylate and subsequent cross-linking with an amino-terminated aggregation-induced emission fluorogen. Such obtained amphiphilic copolymers can self-assemble to form uniform fluorescent polymeric nanoparticles (FPNs) and be utilized for cell imaging. These cross-linked FPNs are demonstrated good water dispersibility with ultralow critical micelle concentration (∼ 0.002 mg mL(-1)), uniform morphology (98 ± 2 nm), high red fluorescence quantum yield, and excellent biocompatibility. More importantly, this novel strategy of fabricating cross-linked FPNs paves the way to the future development of more robust and biocompatible fluorescent bioprobes.
authors: College of Chemistry and Pharmaceutical Sciences, Qingdao Agriculture University , Qingdao, 266109, P. R. China.
authors: Haiyin
authors: H
authors: Li
authors: Xiqi
authors: X
authors: Zhang
authors: Xiaoyong
authors: X
authors: Zhang
authors: Ke
authors: K
authors: Wang
authors: Hongliang
authors: H
authors: Liu
authors: Yen
authors: Y
authors: Wei
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002460:Cell Line; D002477:Cells / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D017953:Isocyanates / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D057054:Molecular Imaging / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D017953:Isocyanates; D008689:Methacrylates; D011108:Polymers; D011092:Polyethylene Glycols; C029845:2-isocyanatoethyl methacrylate
keywords: PEGylation; aggregation-induced emission; cell imaging; critical micelle concentration; cross-linking; fluorescent polymeric nanoparticles