title: Trip6 promotes dendritic morphogenesis through dephosphorylated GRIP1-dependent myosin VI and F-actin organization.
abstract: Thyroid receptor-interacting protein 6 (Trip6), a multifunctional protein belonging to the zyxin family of LIM proteins, is involved in various physiological and pathological processes, including cell migration and tumorigenesis. However, the role of Trip6 in neurons remains unknown. Here, we show that Trip6 is expressed in mouse hippocampal neurons and promotes dendritic morphogenesis. Through interaction with the glutamate receptor-interacting protein 1 (GRIP1) and myosin VI, Trip6 is crucial for the total dendritic length and the number of primary dendrites in cultured hippocampal neurons. Trip6 depletion reduces F-actin content and impairs dendritic morphology, and this phenocopies GRIP1 or myosin VI knockdown. Furthermore, phosphorylation of GRIP1(956T) by AKT1 inhibits the interaction between GRIP1 and myosin VI, but facilitates GRIP1 binding to 14-3-3 protein, which is required for regulating F-actin organization and dendritic morphogenesis. Thus, the Trip6-GRIP1-myosin VI interaction and its regulation on F-actin network play a significant role in dendritic morphogenesis.
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Kaosheng
authors: K
authors: Lv
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Liang
authors: L
authors: Chen
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Yuanjun
authors: Y
authors: Li
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Zenglong
authors: Z
authors: Li
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Pengli
authors: P
authors: Zheng
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and.
authors: Yingying
authors: Y
authors: Liu
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and Center for Quantitative Biology, Peking University, Beijing 100871, China junlinteng@pku.edu.cn chenjg@pku.edu.cn.
authors: Jianguo
authors: J
authors: Chen
authors: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, College of Life Sciences, and junlinteng@pku.edu.cn chenjg@pku.edu.cn.
authors: Junlin
authors: J
authors: Teng
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000074183:ATPases Associated with Diverse Cellular Activities; D000199:Actins / Q000502:physiology*; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D003712:Dendrites / Q000648:ultrastructure*; D057809:HEK293 Cells; D006624:Hippocampus / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D060588:LIM Domain Proteins / Q000502:physiology*; D009024:Morphogenesis; D018995:Myosin Heavy Chains / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009474:Neurons / Q000648:ultrastructure; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D048948:14-3-3 Proteins; D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C491310:GRIP1 protein, human; D060588:LIM Domain Proteins; D009419:Nerve Tissue Proteins; C092282:PSMC5 protein, human; D014157:Transcription Factors; C089785:myosin VI; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt; D046988:Proteasome Endopeptidase Complex; D000074183:ATPases Associated with Diverse Cellular Activities; D018995:Myosin Heavy Chains
keywords: 14-3-3; F-actin organization; GRIP1; Trip6; dendritic morphogenesis; myosin VI