title: Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe.
abstract: Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors: Dabin
authors: D
authors: Kim
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors: Gyoungmi
authors: G
authors: Kim
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors: Sang-Jip
authors: SJ
authors: Nam
authors: 1] Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea [2] Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan. 430079, P. R. China.
authors: Jun
authors: J
authors: Yin
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors: Juyoung
authors: J
authors: Yoon
mesh_terms: D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide