title: Iron dextran increases hepatic oxidative stress and alters expression of genes related to lipid metabolism contributing to hyperlipidaemia in murine model.
abstract: The objective of this study was to investigate the effects of iron dextran on lipid metabolism and to determine the involvement of oxidative stress. Fischer rats were divided into two groups: the standard group (S), which was fed the AIN-93M diet, and the standard plus iron group (SI), which was fed the same diet but also received iron dextran injections. Serum cholesterol and triacylglycerol levels were higher in the SI group than in the S group. Iron dextran was associated with decreased mRNA levels of pparα, and its downstream gene cpt1a, which is involved in lipid oxidation. Iron dextran also increased mRNA levels of apoB-100, MTP, and L-FABP indicating alterations in lipid secretion. Carbonyl protein and TBARS were consistently higher in the liver of the iron-treated rats. Moreover, a significant positive correlation was found between oxidative stress products, lfabp expression, and iron stores. In addition, a negative correlation was found between pparα expression, TBARS, carbonyl protein, and iron stores. In conclusion, our results suggest that the increase observed in the transport of lipids in the bloodstream and the decreased fatty acid oxidation in rats, which was promoted by iron dextran, might be attributed to increased oxidative stress.
authors: Basic Department-Health Area, Federal University of Juiz de Fora, Campus Universitário s/n, 36036-900 Juiz de Fora, MG, Brazil.
authors: Maísa
authors: M
authors: Silva
authors: Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil.
authors: Joyce Ferreira
authors: JF
authors: da Costa Guerra
authors: Program of Health and Nutrition, Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil.
authors: Ana Flávia Santos
authors: AF
authors: Sampaio
authors: Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil ; Department of Biological Sciences, Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil.
authors: Wanderson Geraldo
authors: WG
authors: de Lima
authors: Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil ; Department of Foods, Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil.
authors: Marcelo Eustáquio
authors: ME
authors: Silva
authors: Research Center in Biological Sciences (NUPEB), Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil ; Department of Biological Sciences, Federal University of Ouro Preto, Campus Morro do Cruzeiro s/n, 35400-000 Ouro Preto, MG, Brazil.
authors: Maria Lucia
authors: 0000-0002-6128-1745
authors: ML
authors: Pedrosa
mesh_terms: D000818:Animals; D053299:Apolipoprotein B-100 / Q000096:biosynthesis; D002352:Carrier Proteins / Q000096:biosynthesis; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006397:Hematinics / Q000009:adverse effects* / Q000494:pharmacology; D006949:Hyperlipidemias / Q000378:metabolism* / Q000473:pathology; D007505:Iron-Dextran Complex / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D053299:Apolipoprotein B-100; D002352:Carrier Proteins; C493320:Fabp1 protein, mouse; D050556:Fatty Acid-Binding Proteins; D006397:Hematinics; C106092:microsomal triglyceride transfer protein; D007505:Iron-Dextran Complex