title: The combination of resveratrol and quercetin enhances the individual effects of these molecules on triacylglycerol metabolism in white adipose tissue.
abstract: OBJECTIVE
  The aim of this study was to analyze whether the combination of resveratrol and quercetin showed additive or synergic effects on body fat accumulation and triacylglycerol metabolism in adipose tissue from rats fed an obesogenic diet.
  
  
  METHODS
  Rats were divided into four dietary groups: a control group and three groups each treated with either resveratrol (15 mg/kg/day; RSV), quercetin (30 mg/kg/day; Q), or both (15 mg resveratrol/kg/day and 30 mg quercetin/kg/day; RSV + Q) for 6 weeks. White adipose tissues from several anatomical locations were dissected. Serum parameters were analyzed by using commercial kits. The activities of fatty acid synthase and heparin-releasable lipoprotein lipase (HR-LPL) were measured using spectrophotometric and fluorimetric methods, and protein expression of acetyl-CoA carboxylase (ACC), adipose tissue triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) by western blot.
  
  
  RESULTS
  The administration of either resveratrol or quercetin separately did not induce significant reductions in adipose tissue weights. By contrast, the combination of both molecules led to a significant reduction in all the fat depots analyzed. The percentage of reduction in each tissue was greater than the calculated additive effect. HR-LPL activity was reduced in RSV and RSV + Q groups. The activity of HSL was not modified. By contrast, ACC was inhibited and ATGL increased only by the combination of both polyphenols.
  
  
  CONCLUSIONS
  The results obtained demonstrate a synergistic effect between resveratrol and quercetin and suggest that when these molecules are combined, a great number of metabolic pathways involved in adipose tissue triacylglycerol accumulation are affected.
authors: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain.|CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain.
authors: Noemí
authors: N
authors: Arias
authors: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain.|CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain.
authors: M Teresa
authors: MT
authors: Macarulla
authors: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain.|CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain.
authors: Leixuri
authors: L
authors: Aguirre
authors: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain.
authors: Iñaki
authors: I
authors: Milton
authors: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain. mariapuy.portillo@ehu.es.|CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain. mariapuy.portillo@ehu.es.
authors: María P
authors: MP
authors: Portillo
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004357:Drug Synergism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D009929:Organ Size; D011794:Quercetin / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D002787:Sterol Esterase / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005230:Fatty Acids, Nonesterified; D013267:Stilbenes; D014280:Triglycerides; D011794:Quercetin; D064429:Fatty Acid Synthases; D002787:Sterol Esterase; D008071:Lipoprotein Lipase; D000103:Acetyl-CoA Carboxylase; D000077185:Resveratrol
keywords: Obesity; Quercetin; Rat; Resveratrol; White adipose tissue