title: Exposure to vancomycin causes a shift in the microbial community structure without affecting nitrate reduction rates in river sediments.
abstract: Antibiotics and antibiotic resistance genes have shown to be omnipresent in the environment. In this study, we investigated the effect of vancomycin (VA) on denitrifying bacteria in river sediments of a Waste Water Treatment Plant, receiving both domestic and hospital waste. We exposed these sediments continuously in flow-through reactors to different VA concentrations under denitrifying conditions (nitrate addition and anoxia) in order to determine potential nitrate reduction rates and changes in sedimentary microbial community structures. The presence of VA had no effect on sedimentary nitrate reduction rates at environmental concentrations, whereas a change in bacterial (16S rDNA) and denitrifying (nosZ) community structures was observed (determined by polymerase chain reaction-denaturing gradient gel electrophoresis). The bacterial and denitrifying community structure within the sediment changed upon VA exposure indicating a selection of a non-susceptible VA population.
authors: Université Rennes 1, CNRS EcoBio UMR6553, Rennes, France, anniet.laverman@univ-rennes1.fr.
authors: Anniet M
authors: AM
authors: Laverman
authors: Thibaut
authors: T
authors: Cazier
authors: Chen
authors: C
authors: Yan
authors: Céline
authors: C
authors: Roose-Amsaleg
authors: Fabienne
authors: F
authors: Petit
authors: Josette
authors: J
authors: Garnier
authors: Thierry
authors: T
authors: Berthe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D024881:Drug Resistance, Bacterial; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics*; D009589:Nitrogen Oxides / Q000737:chemistry; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000737:chemistry / Q000382:microbiology; D014640:Vancomycin / Q000494:pharmacology*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000494:pharmacology*; D018508:Water Purification
chemical_list: D000900:Anti-Bacterial Agents; D009589:Nitrogen Oxides; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D014640:Vancomycin