title: Combinatorial therapy using negative pressure and varying lithium dosage for accelerated wound healing.
abstract: In this work, we investigated the effects of negative pressure, applied using a pump designed for Negative Pressure Wound Therapy (NPWT), on the process of wound healing in vitro via initiation of the Wnt signaling pathway. Results indicate that negative pressure enhanced Wnt signaling and migration into a simulated wound in vitro in NIH-3T3 murine fibroblast cells. Increasing doses of lithium (upto 15 mM) increased basal Wnt signaling and enhanced cell migration into the simulated wound site. A combination of negative pressure and increased doses of lithium synergistically increased Wnt signaling and demonstrated further enhanced cell migration into simulated wound sites, with maximal filling of the simulated wound observed at lithium concentrations of at least 10mM.
authors: Center for Biotechnology and Interdisciplinary Studies, Department of Biomedical Engineering Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: pandiv@rpi.edu.
authors: Vaibhav
authors: V
authors: Pandit
authors: Center for Biotechnology and Interdisciplinary Studies, Department of Biomedical Engineering Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: sterling.nesbitt@gmail.com.
authors: Sterling Robert
authors: SR
authors: Nesbitt
authors: Center for Biotechnology and Interdisciplinary Studies, Department of Biomedical Engineering Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: kimd13@rpi.edu.
authors: Diana Y
authors: DY
authors: Kim
authors: Center for Biotechnology and Interdisciplinary Studies, Department of Biomedical Engineering Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: mixona@rpi.edu.
authors: Amanda
authors: A
authors: Mixon
authors: Center for Biotechnology and Interdisciplinary Studies, Department of Biomedical Engineering Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: kothas2@rpi.edu.
authors: Shiva P
authors: SP
authors: Kotha
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003131:Combined Modality Therapy; D004305:Dose-Response Relationship, Drug; D008094:Lithium / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D054843:Negative-Pressure Wound Therapy; D013997:Time Factors; D015533:Transcriptional Activation / Q000187:drug effects; D060449:Wnt Signaling Pathway / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*; D051176:beta Catenin / Q000235:genetics
chemical_list: D051176:beta Catenin; D008094:Lithium
keywords: Migration into score; Murine fibroblasts; Piezoelectric vacuum pump; Wnt signaling; β-catenin