title: The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.
abstract: Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
authors: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.
authors: John
authors: J
authors: Butler
authors: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.
authors: Neil
authors: N
authors: Smyth
authors: Peptide Protein Research Ltd, 184 Funtley Road, Fareham PO15 6DP, UK.
authors: Robert
authors: R
authors: Broadbridge
authors: Peptide Protein Research Ltd, 184 Funtley Road, Fareham PO15 6DP, UK.
authors: Claire E
authors: CE
authors: Council
authors: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.
authors: Anthony G
authors: AG
authors: Lee
authors: The Clore Laboratory, University of Buckingham, Buckingham MK18 1EG, UK.
authors: Claire J
authors: CJ
authors: Stocker
authors: The Clore Laboratory, University of Buckingham, Buckingham MK18 1EG, UK.
authors: David C
authors: DC
authors: Hislop
authors: The Clore Laboratory, University of Buckingham, Buckingham MK18 1EG, UK.
authors: Jonathan R S
authors: JR
authors: Arch
authors: The Clore Laboratory, University of Buckingham, Buckingham MK18 1EG, UK.
authors: Michael A
authors: MA
authors: Cawthorne
authors: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK. Electronic address: jme1@soton.ac.uk.
authors: J
authors: J
authors: Malcolm East
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics / Q000502:physiology; D059305:Diet, High-Fat; D004734:Energy Metabolism / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D011510:Proteolipids / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D022722:Thermogenesis / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation
chemical_list: D009124:Muscle Proteins; D009842:Oligopeptides; D011510:Proteolipids; D011993:Recombinant Fusion Proteins; C077258:sarcolipin; C085706:FLAG peptide; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
keywords: Energy expenditure; High fat diet; Obesity; SERCA; Sarcolipin; Thermogenesis