title: Fast bi-exponential fluorescence lifetime imaging analysis methods.
abstract: A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are noniterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They are applicable to single-channel or 2D multichannel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed nonfitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.
authors: David Day-Uei
authors: DD
authors: Li
authors: Hongqi
authors: H
authors: Yu
authors: Yu
authors: Y
authors: Chen
mesh_terms: D000465:Algorithms; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods*