title: Detection of Babesia bigemina infection in cattle from north-eastern India by polymerase chain reaction and its genetic relatedness with other isolates.
abstract: A total of 333 blood samples were collected from cattle suspected for haemoprotozoan infections from three states of north-eastern part of India. All the samples were examined for diagnosis of Babesia bigemina infection using PCR for detection of specific DNA. Out of these, 12 (3.60%) samples were found positive for B. bigemina DNA on PCR using the organism-specific primers derived from 18S ribosomal RNA (rRNA) gene of B. bigemina. An expected size of 1124-bp PCR product was visualized on agarose gel electrophoresis with all the 12 samples, and four of the products was further cloned and sequenced. Basic Local Alignment Search Tool (BLAST) analysis of B. bigemina sequences generated in the present study share 99.2 to 99.7% identity at 18S rRNA gene nucleotide sequence level. These Indian B. bigemina sequences were found to be closely related with the cognate gene nucleotide sequences of B. bigemina from Argentina and Kenya where 99.1 to 99.9% and 99.0 to 99.7% nucleotide identities were observed, respectively. Distant relationship of these Indian organisms was observed with few cognate gene sequences from China where more than 7% divergence was observed in the distance matrix.
authors: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, Meghalaya, 793103, India, lahar31@rediffmail.com.
authors: Ramgopal
authors: R
authors: Laha
authors: Bimalendu
authors: B
authors: Mondal
authors: Sanchay Kumar
authors: SK
authors: Biswas
authors: Karam
authors: K
authors: Chand
authors: Meena
authors: M
authors: Das
authors: Diganta
authors: D
authors: Sarma
authors: Anupananda
authors: A
authors: Goswami
authors: Arnab
authors: A
authors: Sen
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology* / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D017931:DNA Primers / Q000032:analysis; D016054:DNA, Protozoan / Q000235:genetics; D007194:India / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000032:analysis
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S