title: Whole blood flow cytometric analysis of Ureaplasma-stimulated monocytes from pregnant women.
abstract: We hypothesised that circulating monocytes of women with vaginal colonisation with Ureaplasma spp., genital microorganisms known to cause inflammation-driven preterm birth, would elicit a tolerised cytokine response to subsequent in vitro Ureaplasma parvum serovar 3 (UpSV3) stimulation. Using multi-parameter flow cytometry, we found no differences with regard to maternal colonisation status in the frequency of TNF-α-, IL-6-, IL-8- and IL-1β-expressing monocytes in response to subsequent UpSV3 stimulation (P > 0.10 for all cytokines). We conclude that vaginal Ureaplasma spp. colonisation does not specifically tolerise monocytes of pregnant women towards decreased responses to subsequent stimulation.
authors: School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: 10911373@student.uwa.edu.au.
authors: Yael D
authors: YD
authors: Friedland
authors: Bendat Family Comprehensive Cancer Centre, St. John of God Subiaco Hospital, Perth, Western Australia, Australia; School of Surgery, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: tracey.lee-pullen@uwa.edu.au.
authors: Tracey F
authors: TF
authors: Lee-Pullen
authors: Women and Infants Research Foundation, Perth, Western Australia, Australia. Electronic address: liz.nathan@uwa.edu.au.
authors: Elizabeth
authors: E
authors: Nathan
authors: School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: 20157536@student.uwa.edu.au.
authors: Rory
authors: R
authors: Watts
authors: School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia; Women and Infants Research Foundation, Perth, Western Australia, Australia. Electronic address: jeff.keelan@uwa.edu.au.
authors: Jeffrey A
authors: JA
authors: Keelan
authors: School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: matthew.payne@uwa.edu.au.
authors: Matthew S
authors: MS
authors: Payne
authors: School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: demelza.ireland@uwa.edu.au.
authors: Demelza J
authors: DJ
authors: Ireland
mesh_terms: D000328:Adult; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D015846:Monokines / Q000097:blood / Q000276:immunology*; D011247:Pregnancy; D047928:Premature Birth / Q000097:blood / Q000276:immunology / Q000382:microbiology; D014509:Ureaplasma / Q000276:immunology*
chemical_list: D015846:Monokines
keywords: Cytokines; Monocytes; Multi-parameter flow cytometry; Preterm birth; Ureaplasma spp.