title: The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes.
abstract: Mass spectrometry (MS) is a powerful tool for determining the mass of biomolecules with high accuracy and sensitivity. MS performed under so-called "native conditions" (native MS) can be used to determine the mass of biomolecules that associate noncovalently. Here we review the application of native MS to the study of protein-ligand interactions and its emerging role in elucidating the structure of macromolecular assemblies, including soluble and membrane protein complexes. Moreover, we discuss strategies aimed at determining the stoichiometry and topology of subunits by inducing partial dissociation of the holo-complex. We also survey recent developments in "native top-down MS", an approach based on Fourier Transform MS, whereby covalent bonds are broken without disrupting non-covalent interactions. Given recent progress, native MS is anticipated to play an increasingly important role for researchers interested in the structure of macromolecular complexes.
authors: Université Grenoble Alpes, Institut de Biologie Structurale (IBS), 71 Avenue des Martyrs, F-38044, Grenoble, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), DSV, IBS, F-38044, Grenoble, France.|Centre National de la Recherche Scientifique (CNRS), IBS, F-38044, Grenoble, France.
authors: Elisabetta
authors: http://orcid.org/0000-0002-5910-4707
authors: E
authors: Boeri Erba
authors: Université Grenoble Alpes, Institut de Biologie Structurale (IBS), 71 Avenue des Martyrs, F-38044, Grenoble, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), DSV, IBS, F-38044, Grenoble, France.|Centre National de la Recherche Scientifique (CNRS), IBS, F-38044, Grenoble, France.
authors: Carlo
authors: C
authors: Petosa
mesh_terms: D000818:Animals; D005583:Fourier Analysis; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008024:Ligands; D046911:Macromolecular Substances; D008565:Membrane Proteins; D011506:Proteins
keywords: 2D interaction map; macromolecular complexes; native mass spectrometry (MS); protein−ligand interactions; stoichiometry; top-down MS