title: Effects of interleukin-13 and montelukast on the expression of zonula occludens-1 in human podocytes.
abstract: OBJECTIVE
  The aim of this study was to investigate whether pathologic changes in zonula occludens-1 (ZO-1) are induced by interleukin-13 (IL-13) in the experimental minimal-change nephrotic syndrome (MCNS) model and to determine whether montelukast, a leukotriene receptor antagonist, has an effect on ZO-1 restoration in cultured human podocytes.
  
  
  METHODS
  Human podocytes cultured on bovine serum albumin-coated plates were treated with different doses of IL-13 and montelukast and then examined for distribution using confocal microscopy and for ZO-1 protein levels using Western blotting.
  
  
  RESULTS
  ZO-1 was internalized and shown to accumulate in the cytoplasm of human podocytes in an IL-13 dose-dependent manner. High doses (50 and 100 ng/mL) of IL-13 decreased the levels of ZO-1 protein at 12 and 24 h (both p<0.01; n=3), which were significantly reversed by a high dose (0.5 μM) montelukast treatment (p<0.01; n=3).
  
  
  CONCLUSIONS
  Our results suggest that IL-13 alters the expression of ZO-1, and such alterations in the content and distribution of ZO-1 may be relevant in the pathogenesis of proteinuria in the MCNS model.
authors: Department of Pediatrics, Ajou University School of Medicine, Suwon, Korea.; Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea.
authors: Se Jin
authors: SJ
authors: Park
authors: Children's and Academic Renal Unit, Southmead Hospital, University of Bristol, Bristol, United Kingdom.
authors: Moin A
authors: MA
authors: Saleem
authors: Department of Pediatrics, Chungbuk National University College of Medicine, Cheongju, Korea.
authors: Ja-Ae
authors: JA
authors: Nam
authors: Department of Pediatrics, Chungbuk National University College of Medicine, Cheongju, Korea. tsha@chungbuk.ac.kr.
authors: Tae-Sun
authors: TS
authors: Ha
authors: Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea. shinji@yuhs.ac.
authors: Jae Il
authors: JI
authors: Shin
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D015153:Blotting, Western; D003521:Cyclopropanes; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D020024:Leukotriene Antagonists / Q000494:pharmacology*; D018613:Microscopy, Confocal; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism; D011507:Proteinuria / Q000473:pathology; D011804:Quinolines / Q000494:pharmacology*; D013440:Sulfides; D019108:Tight Junctions; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D000085:Acetates; D003521:Cyclopropanes; D018793:Interleukin-13; D020024:Leukotriene Antagonists; D011804:Quinolines; D013440:Sulfides; D062826:Zonula Occludens-1 Protein; C093875:montelukast
keywords: Interleukin-13; leukotriene receptor antagonists; podocytes; zonula occludens-1