title: Anchoring dipalmitoyl phosphoethanolamine to nanoparticles boosts cellular uptake and fluorine-19 magnetic resonance signal.
abstract: Magnetic resonance (MR) methods to detect and quantify fluorine ((19)F) nuclei provide the opportunity to study the fate of cellular transplants in vivo. Cells are typically labeled with (19)F nanoparticles, introduced into living organisms and tracked by (19)F MR methods. Background-free imaging and quantification of cell numbers are amongst the strengths of (19)F MR-based cell tracking but challenges pertaining to signal sensitivity and cell detection exist. In this study we aimed to overcome these limitations by manipulating the aminophospholipid composition of (19)F nanoparticles in order to promote their uptake by dendritic cells (DCs). As critical components of biological membranes, phosphatidylethanolamines (PE) were studied. Both microscopy and MR spectroscopy methods revealed a striking (at least one order of magnitude) increase in cytoplasmic uptake of (19)F nanoparticles in DCs following enrichment with 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE). The impact of enriching (19)F nanoparticles with PE on DC migration was also investigated. By manipulating the nanoparticle composition and as a result the cellular uptake we provide here one way of boosting (19)F signal per cell in order to overcome some of the limitations related to (19)F MR signal sensitivity. The boost in signal is ultimately necessary to detect and track cells in vivo.
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Sonia
authors: S
authors: Waiczies
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Stefano
authors: S
authors: Lepore
authors: Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
authors: Karl
authors: K
authors: Sydow
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Susanne
authors: S
authors: Drechsler
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Min-Chi
authors: MC
authors: Ku
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Conrad
authors: C
authors: Martin
authors: Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
authors: Dorothea
authors: D
authors: Lorenz
authors: Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
authors: Irene
authors: I
authors: Schütz
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Henning M
authors: HM
authors: Reimann
authors: Electron Microscopy Core Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Bettina
authors: B
authors: Purfürst
authors: 1] Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany [2] Experimental and Clinical Research Center, Berlin, Germany.
authors: Matthias A
authors: MA
authors: Dieringer
authors: MRI TOOLs GmbH, Berlin, Germany.
authors: Helmar
authors: H
authors: Waiczies
authors: Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
authors: Margitta
authors: M
authors: Dathe
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Andreas
authors: A
authors: Pohlmann
authors: Berlin Ultrahigh Field Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors: Thoralf
authors: T
authors: Niendorf
mesh_terms: D000818:Animals; D001692:Biological Transport; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005461:Fluorine / Q000737:chemistry*; D042541:Intracellular Space / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D010714:Phosphatidylethanolamines; D005461:Fluorine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine