title: Protein-protein interaction network of gene expression in the hydrocortisone-treated keloid.
abstract: OBJECTIVE
  In order to explore the molecular mechanism of hydrocortisone in keloid tissue, the gene expression profiles of keloid samples treated with hydrocortisone were subjected to bioinformatics analysis.
  
  
  METHODS
  Firstly, the gene expression profiles (GSE7890) of five samples of keloid treated with hydrocortisone and five untreated keloid samples were downloaded from the Gene Expression Omnibus (GEO) database. Secondly, data were preprocessed using packages in R language and differentially expressed genes (DEGs) were screened using a significance analysis of microarrays (SAM) protocol. Thirdly, the DEGs were subjected to gene ontology (GO) function and KEGG pathway enrichment analysis. Finally, the interactions of DEGs in samples of keloid treated with hydrocortisone were explored in a human protein-protein interaction (PPI) network, and sub-modules of the DEGs interaction network were analyzed using Cytoscape software.
  
  
  RESULTS
  Based on the analysis, 572 DEGs in the hydrocortisone-treated samples were screened; most of these were involved in the signal transduction and cell cycle. Furthermore, three critical genes in the module, including COL1A1, NID1, and PRELP, were screened in the PPI network analysis.
  
  
  CONCLUSIONS
  These findings enhance understanding of the pathogenesis of the keloid and provide references for keloid therapy.
authors: Department of Plastic and Reconstructive Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors: Rui
authors: R
authors: Chen
authors: Zhiliang
authors: Z
authors: Zhang
authors: Zhujia
authors: Z
authors: Xue
authors: Lin
authors: L
authors: Wang
authors: Mingang
authors: M
authors: Fu
authors: Yi
authors: Y
authors: Lu
authors: Ling
authors: L
authors: Bai
authors: Ping
authors: P
authors: Zhang
authors: Zhihong
authors: Z
authors: Fan
mesh_terms: D005786:Gene Expression Regulation; D006801:Humans; D006854:Hydrocortisone / Q000627:therapeutic use*; D007627:Keloid / Q000188:drug therapy* / Q000235:genetics*; D060066:Protein Interaction Maps
chemical_list: D006854:Hydrocortisone