title: Protective activity of Dendropanax morbifera against cisplatin-induced acute kidney injury.
abstract: OBJECTIVE
  Drug-induced acute kidney injury (AKI) has been a severe threat to hospitalized patients, raising the urgent needs to develop strategies to reduce AKI. We investigated the protective activity of Dendropanax morbifera (DP), a medicinal plant which has been widely used to treat infectious and pain diseases, on acute kidney injury (AKI) using cisplatin-induced nephropathic models.
  
  
  METHODS
  Both in vitro renal tubular cells (NRK-52E) and in vivo rat models were used to demonstrate the nephroprotective effect of DP.
  
  
  RESULTS
  Methanolic extract from DP significantly reduced cisplatin-induced toxicity in renal tubular cells. Through successive liquid extraction, the extract of DP was separated into n-hexane, CHCl3, EtOAc, n-BuOH, and H2O fractions. Among these, the CHCl3 fraction (DPCF) was found to be most potent. The protective activity of DPCF was found to be mediated through anti-oxidant, mitochondrial protective, and anti-apoptotic activities. In in vivo rat models of AKI, treatment with DPCF significantly reversed the cisplatin-induced increase in blood urea nitrogen and serum creatinine and histopathologic damage, recovered the level of anti-oxidant enzymes, and inhibited renal apoptosis.
  
  
  CONCLUSIONS
  We demonstrated that DP extracts decreased cisplatin-induced renal toxicity, indicating its potential to ameliorate drug-associated acute kidney damage.
authors: College of Pharmacy Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Republic of Korea.
authors: Eun-Sun
authors: ES
authors: Kim
authors: Jong Soo
authors: JS
authors: Lee
authors: Muhammad
authors: M
authors: Akram
authors: Kyeong-A
authors: KA
authors: Kim
authors: Young-Jun
authors: YJ
authors: Shin
authors: Jong-Hyun
authors: JH
authors: Yu
authors: Ok-Nam
authors: ON
authors: Bae
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D027481:Araliaceae; D002460:Cell Line; D002945:Cisplatin / Q000633:toxicity*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010936:Plant Extracts; D002945:Cisplatin