title: Nanoscale control of surface immobilized BMP-2: toward a quantitative assessment of BMP-mediated signaling events.
abstract: In this work we determine the impact of surface density of immobilized BMP-2 on intracellular signal transduction. We use block copolymer micellar nanolithography to fabricate substrates with precisely spaced and tunable gold nanoparticle arrays carrying single BMP-2 molecules. We found that the immobilized growth factor triggers prolonged and elevated Smad signaling pathway activation compared to the same amount of soluble protein. This approach is suitable for achieving controlled and sustained local delivery of BMP-2 and other growth factors.
authors: Department of Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg , INF 253, 69120 Heidelberg, Germany.
authors: Elisabeth H
authors: EH
authors: Schwab
authors: Theresa L M
authors: TL
authors: Pohl
authors: Tamás
authors: T
authors: Haraszti
authors: Gerburg K
authors: GK
authors: Schwaerzer
authors: Christian
authors: C
authors: Hiepen
authors: Joachim P
authors: JP
authors: Spatz
authors: Petra
authors: P
authors: Knaus
authors: Elisabetta A
authors: EA
authors: Cavalcanti-Adam
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002460:Cell Line; D003460:Crystallization / Q000379:methods; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D066330:Printing, Three-Dimensional; D011485:Protein Binding; D015398:Signal Transduction / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D011092:Polyethylene Glycols; D006046:Gold
keywords: BCMN; BMP-2; cell signaling; covalent immobilization; gold nanostructures