title: Development and characterization of polymorphic microsatellite markers (SSRs) for an endemic plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae).
abstract: Pseudolarix (Pinaceae) is a vulnerable (sensu IUCN) monotypic genus restricted to southeastern China. To better understand levels of genetic diversity, population structure and gene flow among populations of P. amabilis, we developed five compound SSR markers and ten novel polymorphic expressed sequence tags (EST) derived microsatellites. The results showed that all 15 loci were polymorphic with the number of alleles per locus ranging from two to seven. The expected and observed heterozygosities varied from 0.169 to 0.752, and 0.000 to 1.000, respectively. The inbreeding coefficient ranged from -0.833 to 1.000. These markers will contribute to research on genetic diversity and population genetic structure of P. amabilis, which in turn will contribute to the species conservation.
authors: School of Life Science, Nanjing University, Nanjing 210093, China. gengqifang@126.com.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. gengqifang@126.com.
authors: Qi-Fang
authors: QF
authors: Geng
authors: School of Life Science, Nanjing University, Nanjing 210093, China. liujun.mc@163.com.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. liujun.mc@163.com.
authors: Jun
authors: J
authors: Liu
authors: School of Life Science, Nanjing University, Nanjing 210093, China. 1988bingshan@126.com.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. 1988bingshan@126.com.
authors: Lin
authors: L
authors: Sun
authors: International Center for Tropical Botany, Department of Earth and Environment, Florida International University, Miami, FL 33199, USA. hliu@fiu.edu.|College of Forestry, Guangxi University, Nanning 530004, China. hliu@fiu.edu.
authors: Hong
authors: H
authors: Liu
authors: School of Life Science, Nanjing University, Nanjing 210093, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China.
authors: Yan
authors: Y
authors: Ou-Yang
authors: School of Life Science, Nanjing University, Nanjing 210093, China. caiying79@126.com.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. caiying79@126.com.
authors: Ying
authors: Y
authors: Cai
authors: College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China. xstang@hsu.edu.cn.
authors: Xin-Sheng
authors: XS
authors: Tang
authors: Administration of Zhejiang Qingliangfeng National Nature Reserve, Lin'an 311321, China. laqlf@163.com.
authors: Hong-Wei
authors: HW
authors: Zhang
authors: School of Life Science, Nanjing University, Nanjing 210093, China. wangzs@nju.edu.cn.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. wangzs@nju.edu.cn.
authors: Zhong-Sheng
authors: ZS
authors: Wang
authors: School of Life Science, Nanjing University, Nanjing 210093, China. anshq@nju.edu.cn.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China. anshq@nju.edu.cn.
authors: Shu-Qing
authors: SQ
authors: An
mesh_terms: D001483:Base Sequence; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D028201:Pinaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers