title: Homotypic clustering of OsMYB4 binding site motifs in promoters of the rice genome and cellular-level implications on sheath blight disease resistance.
abstract: The promoter regions (1 kb upstream sequences) of 45,836 annotated genes of rice were analyzed for the presence of OsMYB4 binding sites using a Perl program algorithm. Based on the homotypic clustering concept, 113 promoters were found to have more than 4 binding site motifs. Among the downstream genes of these promoters, five genes which are known to have a role in disease resistance were selected and the binding capacity of OsMYB4 protein in the promoter regions was analyzed by docking studies. Expression level of these genes was analyzed by RT-PCR in Rhizoctonia solani infected rice seedlings. Upon pathogen challenge, higher expression of aminotransferase, ankyrin and WRKY 12 genes was observed corresponding to higher expression of Osmyb4. Over-expression of Osmyb4 cDNA in rice leaf tissues by agro-infection failed to result in similar over-expression of aminotransferase, ankyrin and WRKY 12 as expected. Although the role of OsMYB4 in sheath blight resistance was found to be definitive based on our initial results, artificial over-expression of this TF was observed to be insufficient in regulating the disease resistance related genes.
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: Singh
authors: S
authors: Pooja
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: Kumari
authors: K
authors: Sweta
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: A
authors: A
authors: Mohanapriya
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: C
authors: C
authors: Sudandiradoss
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: Ramamoorthy
authors: R
authors: Siva
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
authors: Kodiveri M
authors: KM
authors: Gothandam
authors: School of Bio Sciences and Technology, VIT University, Vellore 632014, India. Electronic address: babu.s@vit.ac.in.
authors: Subramanian
authors: S
authors: Babu
mesh_terms: D000465:Algorithms; D001665:Binding Sites; D019295:Computational Biology; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D062105:Molecular Docking Simulation; D012275:Oryza / Q000235:genetics* / Q000276:immunology*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012232:Rhizoctonia / Q000502:physiology
chemical_list: D010940:Plant Proteins
keywords: Homotypic clusters; OsMYB4; Rice; Sheath blight; Transcription factor