title: Analysis of dermal papilla cell interactome using STRING database to profile the ex vivo hair growth inhibition effect of a vinca alkaloid drug, colchicine.
abstract: Dermal papillae (DPs) control the formation of hair shafts. In clinical settings, colchicine (CLC) induces patients' hair shedding. Compared to the control, the ex vivo hair fiber elongation of organ cultured vibrissa hair follicles (HFs) declined significantly after seven days of CLC treatment. The cultured DP cells (DPCs) were used as the experimental model to study the influence of CLC on the protein dynamics of DPs. CLC could alter the morphology and down-regulate the expression of alkaline phosphatase (ALP), the marker of DPC activity, and induce IκBα phosphorylation of DPCs. The proteomic results showed that CLC modulated the expression patterns (fold>2) of 24 identified proteins, seven down-regulated and 17 up-regulated. Most of these proteins were presumably associated with protein turnover, metabolism, structure and signal transduction. Protein-protein interactions (PPI) among these proteins, established by Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database, revealed that they participate in protein metabolic process, translation, and energy production. Furthermore, ubiquitin C (UbC) was predicted to be the controlling hub, suggesting the involvement of ubiquitin-proteasome system in modulating the pathogenic effect of CLC on DPC.
authors: Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chia-yi 621, Taiwan. welovearthurhsia@gmail.com.
authors: Ching-Wu
authors: CW
authors: Hsia
authors: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan. myho@cgmh.org.tw.
authors: Ming-Yi
authors: MY
authors: Ho
authors: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan. haoai@ndmctsgh.edu.tw.
authors: Hao-Ai
authors: HA
authors: Shui
authors: Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chia-yi 621, Taiwan. 00687@cych.org.tw.|Department of Ophthalmology, Chia-yi Christian Hospital, Chia-yi 600, Taiwan. 00687@cych.org.tw.
authors: Chong-Bin
authors: CB
authors: Tsai
authors: Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chia-yi 621, Taiwan. biomjt@ccu.edu.tw.
authors: Min-Jen
authors: MJ
authors: Tseng
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D030562:Databases, Protein; D004734:Energy Metabolism; D005786:Gene Expression Regulation; D018859:Hair Follicle / Q000187:drug effects / Q000254:growth & development*; D009924:Organ Culture Techniques; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014738:Vibrissae / Q000166:cytology* / Q000378:metabolism
chemical_list: D020543:Proteome; D003078:Colchicine