title: Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters.
abstract: A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag(+)) is developed to detect Ag(+). The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag(+) induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag(+)-triggered fluorescence switch, we successfully detected Ag(+) at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag(+) in the Silmazin(®), which is dermatological burn ointment having silver sulfadiazine. Ag(+) detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.
authors: Center for Self-Assembly and Complexity, Institute for Basic Science, and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea.
authors: Jihyun
authors: J
authors: Lee
authors: Center for Self-Assembly and Complexity, Institute for Basic Science, and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea.
authors: Juhee
authors: J
authors: Park
authors: Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea.
authors: Hong
authors: H
authors: Hee Lee
authors: School of Integrative Engineering, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Hansoo
authors: H
authors: Park
authors: Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea.
authors: Hugh I
authors: HI
authors: Kim
authors: Center for Self-Assembly and Complexity, Institute for Basic Science, and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea. Electronic address: wjkim@postech.ac.kr.
authors: Won Jong
authors: WJ
authors: Kim
mesh_terms: D015374:Biosensing Techniques; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007477:Ions / Q000737:chemistry / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
chemical_list: D007477:Ions; D012834:Silver; D004247:DNA
keywords: DNA-template silver nanoclusters; Fluorescence switch; Fluorescent probes; Silver ion detection