title: BuShenYiQi granule inhibits atopic dermatitis via improving central and skin Hypothalamic-Pituitary-Adrenal axis function.
abstract: BACKGROUND
  Dysfunction of central and skin Hypothalamic-Pituitary-Adrenal (HPA) axis play important roles in pathogenesis of atopic dermatitis (AD). Our previous studies showed that several Chinese herbs could improve HPA axis function. In this study, we evaluated the anti-inflammatory effects of BuShenYiQi granule (BSYQ), a Chinese herbs formula, in AD mice and explored the effective mechanism from regulation of HPA axis.
  
  
  METHODS
  The ovalbumin (OVA) induced AD mice model were established and treated with BSYQ. We evaluated dermatitis score and histology analysis of dorsal skin lesions, meanwhile, serum corticosterone (CORT), adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH) and inflammatory cytokines were determined by ELISA. The changes of CRH/proopiomelanocortin(POMC) axis elements, corresponding functional receptors and crucial genes of glucocorticosteroidogenesis in the skin were measured by quantitative real-time PCR and western blot, respectively.
  
  
  RESULTS
  The symptoms and pathological changes in skin of AD mice were significantly improved and several markers of inflammation and allergy descended obviously after BSYQ treatment. We found that AD mice had insufficient central HPA tone, but these conditions were markedly improved after BSYQ treatment. The AD mice also showed a disturbed expression of skin HPA. In lesion skin of AD mice, the mRNA and protein expressions of CRH decreased significantly, on the contrary, POMC and cytochrome P450 side-chain cleavage enzyme (CYP11A1) increased markedly, meanwhile, NR3C1 (mouse GR), CRHR2 and 11-hydroxylase type 1(CYP11B1) were reduced locally. Most of these tested indexes were improved after BSYQ treatment.
  
  
  CONCLUSIONS
  AD mice displayed the differential expression pattern of central and skin HPA axis and BSYQ treatment significantly alleviated the symptoms of AD mice and presented anti-inflammatory and anti-allergic effects via regulating the expression of central and skin HPA axis.
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Lingwen
authors: L
authors: Kong
authors: Jinfeng
authors: J
authors: Wu
authors: Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China.
authors: Jingfeng
authors: J
authors: Wu
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Yanhua
authors: Y
authors: Lin
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Genfa
authors: G
authors: Wang
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Jia
authors: J
authors: Wang
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Jiaqi
authors: J
authors: Liu
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Meixia
authors: M
authors: Chen
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Xin
authors: X
authors: Du
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Jing
authors: J
authors: Sun
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Jinpei
authors: J
authors: Lin
authors: Department of integrated traditional Chinese and western medicine, Huashan Hospital, Fudan University, Shanghai, PR China; Institute of integrated traditional Chinese and western medicine of Fudan University, Shanghai, PR China.
authors: Jingcheng
authors: J
authors: Dong
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000097:blood; D016207:Cytokines / Q000097:blood; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D012879:Skin Physiological Phenomena / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; C000594494:bushenyiqi; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone