title: Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
abstract: O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia. Electronic address: zdorovenkoe@mail.ru.
authors: Evelina L
authors: EL
authors: Zdorovenko
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
authors: Alexander S
authors: AS
authors: Shashkov
authors: S.N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors: Marina V
authors: MV
authors: Zhurina
authors: S.N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors: Vladimir K
authors: VK
authors: Plakunov
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D018441:Biofilms / Q000254:growth & development; D002240:Carbohydrate Sequence; D019081:O Antigens / Q000737:chemistry*; D010933:Plankton / Q000254:growth & development; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods; D011549:Pseudomonas / Q000502:physiology*
chemical_list: D019081:O Antigens; D011135:Polysaccharides, Bacterial
keywords: Biofilm; Lipopolysaccharide; O-Polysaccharide structure; Planktonic form; Pseudomonas chlororaphis