title: Undiagnosed leptospirosis cases in naïve and vaccinated dogs: properties of a serological test based on a synthetic peptide derived from Hap1/LipL32 (residues 154-178).
abstract: Leptospirosis is a common disease in dogs, despite having current vaccinations. However, leptospirosis diagnosis based on the routine Microscopic Agglutination Test (MAT) leads to confusing conclusions, especially for infected vaccinated dogs. Indeed, both bacterin and natural infection stimulate the production of agglutinating antibodies. In experimentally infected dogs, antibodies against the peptide PP derived from Hap1/Lipl32 were raised earlier than agglutinating antibodies. The background level of these antibodies was determined in a group of 109 healthy dogs, either vaccinated or not against leptospirosis, with a specificity for IgM of 96.4% and for IgG of 95.5%. PP ELISA was subsequently performed with 118 sera from dogs with suspected leptospirosis that was not confirmed by MAT. New leptospirosis cases based on the PP ELISA results were suspected in 14 out of 102 vaccinated dogs and in two out of 16 non-vaccinated dogs. These results highlight the importance of serological diagnosis corresponding to an interesting window when it is too late for PCR detection and too early to be confirmed by MAT.
authors: Ecole Nationale Vétérinaire, ONIRIS, route de Gachet, BP 40706, 44307 Nantes Cedex 3, France. Electronic address: andre-fontaine@wanadoo.fr.
authors: Geneviève
authors: G
authors: Andre-Fontaine
authors: Ecole Nationale Vétérinaire, ONIRIS, route de Gachet, BP 40706, 44307 Nantes Cedex 3, France. Electronic address: florence_aviat@yahoo.fr.
authors: Florence
authors: F
authors: Aviat
authors: Secteur vétérinaire de Marseille, BP 30182, 13009 Marseille Cedex 9, France. Electronic address: jean-lou.marie@wanadoo.fr.
authors: Jean-Lou
authors: JL
authors: Marie
authors: VIRBAC SA, No. 13 rue LID, 06511 Carros, France. Electronic address: benoit.chatrenet@virbac.com.
authors: Benoit
authors: B
authors: Chatrenet
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005849:Gerbillinae; D007921:Leptospira interrogans / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis / Q000662:veterinary*; D008074:Lipoproteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins; D010446:Peptide Fragments
keywords: Dog; Hap1/LipL32; Leptospirosis; MAT; OMPL; Serological diagnosis