title: Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China.
abstract: The aim of the study was to investigate the prevalence, distribution, and diversity of Escherichia coli in goat-milk-powder plants in Shaanxi, China. Three plants manufacturing goat milk powder in Shaanxi province were visited once for sampling during 2012 and 2013. Samples were taken for isolation of E. coli. Isolates were characterized by antimicrobial susceptibility testing and detection of virulence genes. All isolates were further examined by pulsed-field gel electrophoresis analysis. In total, 53 E. coli strains were isolated from 32 positive samples out of 534 samples. Among E. coli isolates, resistance was most frequently observed to trimethoprim-sulfamethoxazole (75.5%), whereas all isolates were sensitive to gatifloxacin, kanamycin, amikacin, and amoxicillin-clavulanate. The 6 virulence genes of pathogenic E. coli were not detected. Pulsed-field gel electrophoresis results showed that E. coli strains in plants were genetically diverse and milk storage tank could be an important contamination source. This study could provide useful information for plants manufacturing goat milk powder to establish proper management practices that help minimize the chance of microbial contamination.
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Meili
authors: M
authors: Xi
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yuqing
authors: Y
authors: Feng
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Qiong
authors: Q
authors: Li
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Qinnan
authors: Q
authors: Yang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Baigang
authors: B
authors: Zhang
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Guanghui
authors: G
authors: Li
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Chao
authors: C
authors: Shi
authors: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: foodscixiaodong@yahoo.com.
authors: Xiaodong
authors: X
authors: Xia
mesh_terms: D000818:Animals; D002681:China; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005511:Food Handling; D005516:Food Microbiology; D006041:Goats; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D012703:Serotyping
keywords: Escherichia coli; antimicrobial resistance; goat milk powder; virulence gene