title: Up-regulation of ECT2 is associated with poor prognosis in gastric cancer patients.
abstract: OBJECTIVE
  The aim of this study was to investigate the expression of ECT2 in gastric cancer and its clinical significance.
  
  
  RESULTS
  We investigated the differentially expressed genes between gastric cancer tissues and normal gastric mucosa by cDNA microarray, and then we found ECT2 was up-regulated in gastric cancer. What is more, we verified ECT2 expression level by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and measured its protein level by immunohistochemistry (IHC). qRT-PCR analysis indicated ECT2 was significantly up-regulated in gastric cancer and Immunohistochemistry confirmed the percentage of ECT2-positive specimens was significantly higher in gastric carcinoma than in non-tumor tissues. Up-regulation of ECT2 is associated with the degree of histological differentiation (P = 0.007), invasion depth (P = 0.047), lymph node metastasis (P = 0.016), distant metastasis (P = 0.021) and TNM stage (P = 0.016), patients with up-regulated ECT2 had a lower overall survival rate (P = 0.000). Cox regression analysis revealed that up-regulation of ECT2 is an independent prognostic factor in gastric cancer patients (P = 0.012).
  
  
  CONCLUSIONS
  Up-regulation of ECT2 might contribute to the progression of gastric carcinogenesis and may be a useful prognostic indicator in gastric cancer.
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Yi
authors: Y
authors: Jin
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Yuhui
authors: Y
authors: Yu
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Qinshu
authors: Q
authors: Shao
authors: Key Laboratory of Gastroenterology, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Yingyu
authors: Y
authors: Ma
authors: Key Laboratory of Gastroenterology, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Ruxuan
authors: R
authors: Zhang
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Haibo
authors: H
authors: Yao
authors: Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital Hangzhou 310014, Zhejiang, China.
authors: Yuan
authors: Y
authors: Xu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000401:mortality / Q000473:pathology*; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C489850:ECT2 protein, human; D011518:Proto-Oncogene Proteins
keywords: ECT2; gastric cancer; microarray; prognosis