title: Basic fibroblast growth factor promotes macaque follicle development in vitro.
abstract: Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF-FSH combination promotes nonhuman primate primordial follicle development in vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: C L
authors: CL
authors: Lu
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: J
authors: J
authors: Yan
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: X
authors: X
authors: Zhi
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: X
authors: X
authors: Xia
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: T R
authors: TR
authors: Wang
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: L Y
authors: LY
authors: Yan
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: Y
authors: Y
authors: Yu
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: T
authors: T
authors: Ding
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: J M
authors: JM
authors: Gao
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China.
authors: R
authors: R
authors: Li
authors: Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China Department of Obstetrics and GynecologyMedical Center for Human Reproduction, Peking University Third Hospital, Beijing 100191, ChinaKey Laboratory of Assisted ReproductionMinistry of Education, Beijing, ChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductionBeijing, ChinaCenter for Reproductive MedicinePeking University Shenzhen Hospital, No. 1120 Lotus Road, FuTian District, Shenzhen, Guangdong 518000, China jie.qiao@263.net.
authors: J
authors: J
authors: Qiao
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006728:Hormones / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008251:Macaca; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D006728:Hormones; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D005640:Follicle Stimulating Hormone