title: Immunolocalization of dentin matrix protein-1 in human primary teeth treated with different pulp capping materials.
abstract: The aim of this study was to evaluate the immunolocalization of dentin matrix protein (DMP)-1 in human primary teeth treated with different pulp capping materials. Twenty-five primary molars were divided into the following groups: formocresol (FC), calcium hydroxide (CH), mineral trioxide aggregate (MTA), corticosteroid/antibiotic solution + CH (O + CH), and Portland cement (PC), and all received conventional pulpotomy treatment. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP-1. Statistical analysis was performed using the χ(2) test (p < 0.05). Histological analysis revealed statistically significant differences in the comparison among the groups through the use of a score system regarding the presence of hard tissue barrier, odontoblastic layer, and internal resorption, but not regarding pulp calcification. Immunohistochemical analysis showed immunostaining for DMP-1 in groups CH, MTA, O + CH, and PC. Internal resorption was observed in the groups FC and CH. MTA and PC showed pulp repair without inflammation and with the presence of hard tissue barrier. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials.
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Natalino
authors: N
authors: Lourenço Neto
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Nádia C T
authors: NC
authors: Marques
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Ana Paula
authors: AP
authors: Fernandes
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Camila O
authors: CO
authors: Rodini
authors: Department of Clinics and Surgery, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
authors: Vivien T
authors: VT
authors: Sakai
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Ruy Cesar C
authors: RC
authors: Abdo
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Maria Aparecida A M
authors: MA
authors: Machado
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Carlos F
authors: CF
authors: Santos
authors: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Thais M
authors: TM
authors: Oliveira
mesh_terms: D002648:Child; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism*; D057872:Pulp Capping and Pulpectomy Agents / Q000009:adverse effects / Q000494:pharmacology*; D014094:Tooth, Deciduous / Q000378:metabolism* / Q000473:pathology*
chemical_list: C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents
keywords: biomaterials availability; deciduous tooth; immunohistochemistry; pulpotomy