title: A multiple amplification strategy for nucleic acid detection based on host-guest interaction between the β-cyclodextrin polymer and pyrene.
abstract: A multiple amplification strategy has been developed for nucleic acid detection based on host-guest interaction between the β-cyclodextrin polymer (β-CDP) and pyrene. Briefly, the detection system consists of three parts: the polymerase and nicking enzyme-assisted isothermal strand displacement amplification (SDA) activated by a target DNA or microRNA; the exonuclease III-aided cyclic enzymatic amplification (CEA); and the fluorescence enhancement effect based on host-guest interaction between β-CDP and pyrene. This strategy showed a good positive linear correlation with target DNA concentrations in the range from 75 fM to 1 pM with a detection limit of 41 fM. Significantly, our amplification platform was further validated and evaluated successfully by assaying miRNA-21 in human serum. The proposed assay has great potential as a nucleic acid quantification method for use in biomedical research, clinical analysis and disease diagnostics.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn yangxiaohai@hnu.edu.cn.
authors: Xiaochen
authors: X
authors: Guo
authors: Pei
authors: P
authors: Liu
authors: Xiaohai
authors: X
authors: Yang
authors: Kemin
authors: K
authors: Wang
authors: Qing
authors: Q
authors: Wang
authors: Qiuping
authors: Q
authors: Guo
authors: Jin
authors: J
authors: Huang
authors: Jianbo
authors: J
authors: Liu
authors: Chunxia
authors: C
authors: Song
authors: Wenshan
authors: W
authors: Li
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011721:Pyrenes / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D035683:MicroRNAs; D011721:Pyrenes; D047392:beta-Cyclodextrins; C041953:cyclodextrin polymer; D002482:Cellulose; D004247:DNA; C030984:pyrene; C031215:betadex