title: A rapid bioluminescence assay for measuring myeloperoxidase activity in human plasma.
abstract: Myeloperoxidase (MPO) is a circulating cardiovascular disease (CVD) biomarker used to estimate clinical risk and patient prognosis. Current enzyme-linked immunosorbent assays (ELISA) for MPO concentration are costly and time-intensive. Here we report a novel bioluminescence assay, designated MPO activity on a polymer surface (MAPS), for measuring MPO activity in human plasma samples using the bioluminescent substrate L-012. The method delivers a result in under an hour and is resistant to confounding effects from endogenous MPO inhibitors. In a pilot clinical study, we compared MAPS and two clinical ELISAs using 72 plasma samples from cardiac catheterization patients. Results from parallel MAPS and ELISAs were concordant within 2±11 μg l(-1) MPO with similar uncertainty and reproducibility. Results between parallel MAPS and ELISA were in better agreement than those between independent ELISAs. MAPS may provide an inexpensive and rapid assay for determining MPO activity in plasma samples from patients with CVD or potentially other immune and inflammatory disorders.
authors: Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors: Reece J
authors: RJ
authors: Goiffon
authors: Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors: Sara C
authors: SC
authors: Martinez
authors: 1] Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA [2] Department of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, 1400 Pressler Street, Unit 1479, FCT16.6030, Houston, Texas 77030, USA.
authors: David
authors: D
authors: Piwnica-Worms
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002417:Cattle; D057075:Enzyme Assays; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054879:Halogenation / Q000187:drug effects; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000097:blood*; D011108:Polymers / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D015203:Reproducibility of Results; D014019:Tissue Donors
chemical_list: D000975:Antioxidants; D011108:Polymers; C081614:L 012; D008165:Luminol; D009195:Peroxidase; D000645:Ammonium Sulfate