title: Generation and Characterization of Rat iPSCs.
abstract: The laboratory rat (Rattus norvegicus) is now on the leading edge used as a laboratory model system to study pharmacology, toxicology, immunology, nutrition, behavior, and numerous other topics. Therefore, generation of rat induced pluripotent stem cells (iPSCs) through somatic cells reprogramming is a powerful tool for establishing in vitro disease model, development of new protocols for treatment of different diseases, and creating transgenic rat models. Here, we describe a simple adopted protocol for establishing rat iPSCs from different types of somatic cells including rat primary ear fibroblast (PEF) and primary bone marrow cells (BMC).
authors: Broad Institute of MIT and Harvard, Cambridge, MA, USA. jing_liao@harvard.edu.|Harvard Stem Cell Institute, Cambridge, MA, USA. jing_liao@harvard.edu.|Department of Stem Cell and Regenerative Biology, Harvard University, Bauer Laboratories-Administrative Suite, 7 Divinity Avenue, Cambridge, MA, 02138, USA. jing_liao@harvard.edu.
authors: Jing
authors: J
authors: Liao
authors: Wuxi Medical School, Jiangnan University, Wuxi, People's Republic of China.
authors: Chun
authors: C
authors: Cui
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods; D002999:Clone Cells; D004431:Ear, External / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D005822:Genetic Vectors / Q000235:genetics; D064593:Heterografts; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011401:Promoter Regions, Genetic / Q000235:genetics; D017207:Rats, Sprague-Dawley; D013724:Teratoma / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics
chemical_list: D014157:Transcription Factors
keywords: Disease model; Rat; Reprogramming; Somatic cells; iPSCs