title: MicroRNA-29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke.
abstract: OBJECTIVE
  Studies showed fastigial nucleus stimulation (FNS) reduced brain damage, but the mechanisms of neuroprotection induced by FNS were not entirely understood; MicroRNAs are noncoding RNA molecules that regulate gene expression in a posttranscriptional manner, but their functional consequence in response to ischemia-reperfusion (IR) remains unknown. We investigated the role of microRNA-29c in the neuroprotection induced by FNS in rat.
  
  
  METHODS
  The IR rat models were conducted 1 day after FNS. Besides, miR-29c antagomir (or agomir or control) was infused to the left intracerebroventricular 1 day before IR models were conducted. We detected differential expression of Birc2 mRNA (also Bak1mRNA and miR-29c) level among different groups by RT-qPCR. The differential expression of Birc2 protein (also Bak1 protein) level among different groups was surveyed via Western blot. The neuroprotective effects were assessed by infarct volume, neurological deficit, and apoptosis.
  
  
  RESULTS
  MiR-29c was decreased after FNS. Moreover, miR-29c directly bound to the predicted 3'-UTR target sites of Birc2 and Bak1 genes. Furthermore, over-expression of miR-29c effectively reduced Birc2 (also Bak1) mRNA and protein levels, increased infarct volume and apoptosis, and deteriorated neurological outcomes, whereas down-regulation played a neuroprotective role.
  
  
  CONCLUSIONS
  MiR-29c correlates with the neuroprotection induced by FNS by negatively regulating Birc2 and Bak1.
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Li-Gang
authors: LG
authors: Huang
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Jin-Pin
authors: JP
authors: Li
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Xiao-Min
authors: XM
authors: Pang
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Chun-Yong
authors: CY
authors: Chen
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Hui-Yao
authors: HY
authors: Xiang
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Ling-Bo
authors: LB
authors: Feng
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Sheng-You
authors: SY
authors: Su
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Sheng-Hua
authors: SH
authors: Li
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Lei
authors: L
authors: Zhang
authors: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors: Jing-Li
authors: JL
authors: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D020520:Brain Infarction / Q000188:drug therapy / Q000209:etiology; D002529:Cerebellar Nuclei / Q000502:physiology*; D046690:Deep Brain Stimulation / Q000379:methods*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009422:Nervous System Diseases / Q000188:drug therapy / Q000209:etiology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494774:Bak1 protein, rat; D051034:Inhibitor of Apoptosis Proteins; C526366:MIRN29 microRNA, rat; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D050998:bcl-2 Homologous Antagonist-Killer Protein; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; C494836:Birc3 protein, rat
keywords: Apoptosis; Cerebral ischemia; Electrical fastigial nucleus stimulation; MicroRNA