title: Novel method of cell line establishment utilizing fluorescence-activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines.
abstract: The purpose of this study was to present the establishment of new cell lines, which is important to cancer research.
  
  
  
  Six new head and neck squamous cell carcinoma cell lines were established using a novel fluorescence-activated cell sorting (FACS) method in order to overcome the barrier of fibroblast overgrowth and the susceptibility of primary tumors to fail in vitro.
  
  
  
  Antibodies chosen for specific targeting of epithelial cells and fibroblasts successfully separated cells for line establishment in 6 of 12 attempts, providing an alternative method of establishing head and neck squamous cell carcinoma cell lines. Each attempt at cell line establishment resulted in an epithelial carcinoma population, which was genotyped and catalogued as a unique cell line, and a corresponding fibroblast population.
  
  
  
  The selection of antibody markers could be optimized to aid in the establishment of any cancer cell line derived from any tumor tissue; this method is not limited to head and neck cancer. © 2015 Wiley Periodicals, Inc. Head Neck 38: E459-E467, 2016.
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: John Henry
authors: JH
authors: Owen
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Martin P
authors: MP
authors: Graham
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Steven B
authors: SB
authors: Chinn
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Owen F
authors: OF
authors: Darr
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Douglas B
authors: DB
authors: Chepeha
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Gregory T
authors: GT
authors: Wolf
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Carol R
authors: CR
authors: Bradford
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Thomas E
authors: TE
authors: Carey
authors: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
authors: Mark E P
authors: ME
authors: Prince
mesh_terms: D000906:Antibodies; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002469:Cell Separation; D004847:Epithelial Cells; D005347:Fibroblasts; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans
chemical_list: D000906:Antibodies
keywords: cell line; fibroblasts; flow cytometry; squamous cell carcinoma