title: Talbot holographic illumination nonscanning (THIN) fluorescence microscopy.
abstract: Optical sectioning techniques offer the ability to acquire three-dimensional information from various organ tissues by discriminating between the desired in-focus and out-of-focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning.
authors: Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, Taipei, 10051, Taiwan R.O.C.|Molecular Imaging Center, National Taiwan University, Taipei, 10055, Taiwan R.O.C.
authors: Yuan
authors: Y
authors: Luo
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.
authors: Vijay Raj
authors: VR
authors: Singh
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.|Centre for BioImaging Science (CBIS), Blk S1A, Level 2, National University of Singapore, 14 Science Drive 4, 117546, Singapore.|MechanoBiology Institute (MBI), National University of Singapore, 117411, Singapore.
authors: Dipanjan
authors: D
authors: Bhattacharya
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.|Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.
authors: Elijah Y S
authors: EYS
authors: Yew
authors: Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, Taipei, 10051, Taiwan R.O.C.
authors: Jui-Chang
authors: JC
authors: Tsai
authors: Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, 10055, Taiwan R.O.C.
authors: Sung-Liang
authors: SL
authors: Yu
authors: Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, Taipei, 10051, Taiwan R.O.C.|Molecular Imaging Center, National Taiwan University, Taipei, 10055, Taiwan R.O.C.
authors: Hsi-Hsun
authors: HH
authors: Chen
authors: Department of Internal Medicine, National Taiwan University Hospital, Taipei, 10048, Taiwan, R.O.C.
authors: Jau-Min
authors: JM
authors: Wong
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.|Centre for BioImaging Science (CBIS), Blk S1A, Level 2, National University of Singapore, 14 Science Drive 4, 117546, Singapore.|MechanoBiology Institute (MBI), National University of Singapore, 117411, Singapore.
authors: Paul
authors: P
authors: Matsudaira
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.|Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.|Department of Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.
authors: Peter T C
authors: PTC
authors: So
authors: Singapore-MIT Alliance for Research and Technology (SMART) Centre, 1 CREATE Way, #10-01 CREATE Tower, 138602, Singapore.|Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.
authors: George
authors: G
authors: Barbastathis
keywords: 3D wide field Microscopy; Talbot effect; holographic Bragg filters; optical sectioning