title: Recent advances in Citrus psorosis virus.
abstract: Psorosis is a globally devastating disease of citrus caused by an infectious filamentous ophiovirus, Citrus psorosis virus (CPsV), which causes annual losses of about 5 % and a progressive decline of trees by affecting the conductive tissues. The disease can be harboured asymptomatically in many citrus species. In the field, the most characteristic symptoms of the disease in adult trees are bark scaling in the trunk and main branches and also internal staining in the underlying wood. The virus has a tripartite single-stranded RNA genome, and has been inadvertently spread to most citrus growing areas through the movement of citrus propagative material. No natural vectors have been identified except in limited citrus areas in some cases. Management strategies for CPsV involving shoot-tip grafting and thermotherapy or somatic embryogenesis from stigma and style cultures have been successfully used to eliminate CPsV from plant propagating material. Molecular pathogen-mediated strategies have been used to produce citrus plants. Such a strategy protects against infections by the virus from which the resistance gene and promising resistance may emerge from trials. Certification programs are among the best established means of increasing phytosanitary health, and some of those for citrus are among the oldest in the world. In conjunction with quarantine and clean stock programs, they remain important weapons in the ongoing fight against citrus diseases. One of the elements essential for successful certification programs to produce such propagation material is the availability of sensitive and effective diagnostic methods. In this review, we discuss an updated status of CPsV disease.
authors: Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences, Université Ibn Tofaïl, Kénitra, Maroc.
authors: Asmae
authors: A
authors: Achachi
authors: Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UFR Sciences, Université de Reims Champagne-Ardenne, Reims Cédex, France.
authors: Essaïd
authors: E
authors: Ait Barka
authors: Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences, Université Ibn Tofaïl, Kénitra, Maroc.
authors: Mohammed
authors: M
authors: Ibriz
keywords: Certification; Citrus psorosis virus; Diagnostics; Ophiovirus; Segmented negative-stranded RNA virus; Viral resistance