title: Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex.
abstract: Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.
authors: Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
authors: Christopher H S
authors: CH
authors: Aylett
authors: Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
authors: Daniel
authors: D
authors: Boehringer
authors: Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
authors: Jan P
authors: JP
authors: Erzberger
authors: Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
authors: Tanja
authors: T
authors: Schaefer
authors: Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
authors: Nenad
authors: N
authors: Ban
mesh_terms: D001665:Binding Sites; D020285:Cryoelectron Microscopy; D015977:Eukaryotic Initiation Factor-1 / Q000737:chemistry*; D039621:Eukaryotic Initiation Factor-3 / Q000737:chemistry*; D010442:Peptide Chain Initiation, Translational; D017434:Protein Structure, Tertiary; D054682:Ribosome Subunits, Small, Eukaryotic / Q000737:chemistry*; D004718:Saccharomycetales / Q000235:genetics*
chemical_list: D015977:Eukaryotic Initiation Factor-1; D039621:Eukaryotic Initiation Factor-3