title: Dark fermentative bioconversion of glycerol to hydrogen by Bacillus thuringiensis.
abstract: Biodiesel manufacturing units discharge effluents rich in glycerol. The need is to convert crude glycerol (CG) into useful products such as hydrogen (H2). Under batch culture, Bacillusthuringiensis EGU45 adapted on pure glycerol (PG, 2% v/v) resulted in an H2 yield of 0.646 mol/mol glycerol consumed on minimal media (250 mL) supplemented with 1% ammonium nitrate at 37°C over 4 days. Here, H2 constituted 67% of the total biogas. Under continuous culture, at 2 days of hydraulic retention time, B. thuringiensis immobilized on ligno-cellulosic materials (banana leaves - BL, 10% v/v) resulted in a H2 yield of 0.386 mol/mol PG consumed. On CG, the maximal H2 yield of 0.393 mol/mol feed consumed was recorded. In brief, B. thuringiensis could transform CG, on limited resources - minimal medium with sodium nitrate, by immobilizing them on cheap and easily available biowaste, which makes it a suitable candidate for H2 production on a large scale.
authors: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors: Prasun
authors: P
authors: Kumar
authors: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors: Rishi
authors: R
authors: Sharma
authors: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors: Subhasree
authors: S
authors: Ray
authors: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors: Sanjeet
authors: S
authors: Mehariya
authors: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors: Sanjay K S
authors: SKS
authors: Patel
authors: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors: Jung-Kul
authors: JK
authors: Lee
authors: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India. Electronic address: vckalia@igib.res.in.
authors: Vipin C
authors: VC
authors: Kalia
mesh_terms: D001413:Bacillus thuringiensis / Q000737:chemistry / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D018914:Cells, Immobilized; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D028521:Musa / Q000737:chemistry; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D003470:Culture Media; D009566:Nitrates; D006859:Hydrogen; C031618:sodium nitrate; D009584:Nitrogen; D005990:Glycerol
keywords: Adaptation; Bacillus; Continuous culture; Crude glycerol; Dark fermentation