title: A rapid HPLC post-column reaction analysis for the quantification of ergothioneine in edible mushrooms and in animals fed a diet supplemented with extracts from the processing waste of cultivated mushrooms.
abstract: For establishing an efficient and sensitive method for the quantitative determination of 2-thiol-l-histidine-betaine (ergothioneine, ERG) in edible mushrooms and the blood and muscles of animals, a technique using reversed-phase separation and post-column reaction between 2'-dipyridyl disulphide and ERG was developed. A corresponding derivative 2-thiopyridone, detected at 343 nm, was used for estimating ERG concentration. The flow rate, temperature, pH, and composition of the solution were optimised. A low limit of quantification (1.41 ppm) and a simpler sample preparation made this technique more rapid compared to other methods using liquid chromatography-mass spectrometry. The coefficient of variation (CV) values for the reproducibility and recovery of ERG were within the acceptable values of 6% and 97.5-100.0%, respectively. The efficiency of this methodology was compared with that of spectrophotometric and mass-spectrometric quantitative methods, and was assessed in the light of previous studies. The ERG contents in different mushrooms were 12.69-234.85 mg/kg wet weight basis. Dietary supplementation with extracts from mushroom processing waste significantly improved ERG bioavailability in the blood of yellowtail fish and muscle tissue of cattle.
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan.
authors: The Han
authors: TH
authors: Nguyen
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan.
authors: Anupam
authors: A
authors: Giri
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan. Electronic address: tohshima@kaiyodai.ac.jp.
authors: Toshiaki
authors: T
authors: Ohshima
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004032:Diet; D004880:Ergothioneine / Q000032:analysis* / Q000097:blood; D005399:Fishes / Q000378:metabolism; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D014866:Waste Products / Q000032:analysis*
chemical_list: D014866:Waste Products; D004880:Ergothioneine
keywords: Animal blood; Animal muscles; Edible mushrooms; Ergothioneine; HPLC post-column reaction analysis