title: Determination of ethyl carbamate in fermented liquids by ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer.
abstract: In this study, the determination of ethyl carbamate (EC) in fermented liquids (red wines, Chinese liquors and yellow wines) was carried by using ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer (UHPLC-MS/MS). The parameters usually examined in the method validation were firstly evaluated. Good linearity was obtained with a correlation coefficient of 0.9999. The limits of detection (LOD) and quantitation (LOQ) of EC in sample were 1.8 and 4.0 μg/L, respectively. Recoveries ranged between 107.19% and 110.98%. The precision (relative standard deviation, RSD) of the method was <5%. Furthermore, red wines, Chinese liquors and yellow wines for sale were detected using this method. The results showed that the red wines and Chinese liquors had the normal EC level that was within the limits of the Canadian legislation. The proposed method was simple in sample preparation without using organic solvents in pre-treatment and could be used for the determination of the content of EC in fermented liquids.
authors: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China. Electronic address: zhaoxirong1961@163.com.
authors: Xirong
authors: X
authors: Zhao
authors: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China. Electronic address: j1c2x3@gmail.com.
authors: Changxing
authors: C
authors: Jiang
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014520:Urethane / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D014520:Urethane
keywords: Ethyl carbamate; Fermented liquid; Q Exactive hybrid quadrupole-orbitrap mass spectrometer; Ultra high performance liquid chromatography