title: Zinc protects sperm from being damaged by reactive oxygen species in assisted reproduction techniques.
abstract: The aim of this study was to explore the effect of zinc on hydrogen peroxide-induced sperm damage in assisted reproduction techniques. First, sperms were selected from semen samples of 20 healthy men prepared by density gradient centrifugation. Selected sperm were treated with either 0.001% H(2)O(2), 12.5 nM ZnCL(2), 0.001% H(2)O(2) + 12.5 nM ZnCL(2) or 0.9% NaCl(2) (control). After this treatment, the motility, viability, membrane integrity and DNA fragmentation of sperms in each group were analysed by Goodline sperm detection system, optical microscopy and sperm DNA fragmentation assay. Poorer motility, vitality, membrane integrity and more DNA damage were found in sperms treated by H(2)O(2), compared with control. When sperms were treated with both H(2)O(2) and zinc, however, all indicators were improved compared with H(2)O(2) alone. There was a close association between oxidative stimulation and sperm injury; zinc could inhibit hydrogen peroxide-induced damage of sperm in assisted reproductive technology. However, the presence of zinc in culture medium can decrease the sperm quality without addition of peroxide.
authors: Laboratory of Reproduction Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China. Electronic address: pursuer@163.com.
authors: Jinxiang
authors: J
authors: Wu
authors: Department of Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Shiqiang
authors: S
authors: Wu
authors: Department of Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Yuanzhi
authors: Y
authors: Xie
authors: Laboratory of Reproduction Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Zhengyao
authors: Z
authors: Wang
authors: Department of Obstetric and Gynecology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Ruiyun
authors: R
authors: Wu
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Junfeng
authors: J
authors: Cai
authors: Department of Obstetric and Gynecology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Xiangmin
authors: X
authors: Luo
authors: Department of Obstetric and Gynecology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Suzhen
authors: S
authors: Huang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian, China.
authors: Liuxia
authors: L
authors: You
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D002712:Chlorides / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism*; D027724:Reproductive Techniques, Assisted; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D017382:Reactive Oxygen Species; D017967:Zinc Compounds; C016837:zinc chloride; D006861:Hydrogen Peroxide
keywords: DNA damage; assisted reproduction; oxidative stress; reactive oxygen species; sperm