title: Cell death pathway induced by resveratrol-bovine serum albumin nanoparticles in a human ovarian cell line.
abstract: Resveratrol-bovine serum albumin nanoparticles (RES-BSANP) exhibit chemotherapeutic properties, which trigger apoptosis. The aim of the present study was to investigate the caspase-independent cell death pathway induced by RES-BSANP in human ovarian cancer SKOV3 cells and to analyze its mechanism. Morphological changes were observed by apoptotic body/cell nucleus DNA staining using inverted and fluorescence microscopy. The cell death pathway was determined by phosphatidylserine translocation. Western blot analysis was conducted to detect the activation of apoptosis-inducing factor (AIF), cytochromec(Cytoc) and B-cell lymphoma 2-associated X protein (Bax). Apoptotic body and nuclear condensation and fragmentation were observed simultaneously following treatment with RES-BSANP. RES-BSANP induced apoptosis in a dose-dependent manner in the human ovarian cancer SKOV3 cells. The translocation of AIF from the mitochondria to the cytoplasm occurred earlier than that of Cytoc. In addition, Bax binding to the mitochondria was required for the release of AIF and Cytocfrom the mitochondria. The AIF apoptosis pathway may present an alternative caspase-dependent apoptosis pathway in human ovarian cell death induced by RES-BSANP. Elucidation of this pathway may be critical for the treatment of cancer using high doses of RES-BSANP.
authors: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Harbin Medical University, Harbin 150081, P.R. China.
authors: Liyuan
authors: L
authors: Guo
authors: Department of Health, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, P.R. China.
authors: Yan
authors: Y
authors: Peng
authors: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Harbin Medical University, Harbin 150081, P.R. China.
authors: Yulian
authors: Y
authors: Li
authors: Department of Health, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, P.R. China.
authors: Jingping
authors: J
authors: Yao
authors: Department of Health, First Affiliated Hospital, Harbin Medical University, Harbin 150001, P.R. China.
authors: Guangmei
authors: G
authors: Zhang
authors: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Harbin Medical University, Harbin 150081, P.R. China.
authors: Jie
authors: J
authors: Chen
authors: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Harbin Medical University, Harbin 150081, P.R. China.
authors: Jing
authors: J
authors: Wang
authors: Department of Obstetrics and Gynecology, Third Affiliated Hospital, Harbin Medical University, Harbin 150081, P.R. China.
authors: Lihua
authors: L
authors: Sui
keywords: apoptosis; caspase-independent; programmed cell death; resveratrol-bovine serum albumin nanoparticles