title: Bioprocess intensification of antibiotic production by Streptomyces coelicolor A3(2) in micro-porous culture.
abstract: A novel functionalized micro-porous matrix was developed with well-controlled physicochemical proprieties such as pore size and surface chemistry. The matrix was used as a solid support in the growth of "Streptomyces coelicolor" A3(2) to enhance the production of antibiotics. The results shown support a higher production of prodigiosin and actinorhodin with overall production increase of 2-5 and 6-17, respectively, compared to conventional submerged liquid culture, offering a potential improvement in volumetric productivity. Scanning Electron Microscopy was used to evaluate pore size as well as bacterial adhesion, penetration, proliferation and migration within the micro-porous matrix.
authors: NUTRISS Limited, INEX, Herschel Annex, Kings Road, Newcastle upon Tyne NE1 7RU, UK. Electronic address: tm.ndlovu@nutriss.com.
authors: T M
authors: TM
authors: Ndlovu
authors: School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK; Department of Microbiology, Chung-Ang University, College of Medicine, Seoul, Republic of Korea 156-756.
authors: A C
authors: AC
authors: Ward
authors: School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
authors: J
authors: J
authors: Glassey
authors: NUTRISS Limited, INEX, Herschel Annex, Kings Road, Newcastle upon Tyne NE1 7RU, UK.
authors: J
authors: J
authors: Eskildsen
authors: School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
authors: G
authors: G
authors: Akay
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D008855:Microscopy, Electron, Scanning / Q000379:methods; D016062:Porosity; D011353:Prodigiosin / Q000096:biosynthesis / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D011353:Prodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones
keywords: Antibiotic; Functionalization; Micro-porous matrix growth; Polymerisation; Submerged growth; “Streptomyces coelicolor” A3(2)