title: Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae.
abstract: The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup at 293 K and a complete data set was collected to 2.4 Å resolution. The crystal form was orthorhombic (space group P212121), with unit-cell parameters a = 77.2, b = 108.6, c = 167.7 Å. The asymmetric unit contained four molecules with a solvent content of 46.4%.
authors: Institute of Molecular Biology, University of Graz, Humboldtstrasse 50/3, 8010 Graz, Austria.
authors: Srinivasan
authors: S
authors: Rengachari
authors: Institute of Molecular Biology, University of Graz, Humboldtstrasse 50/3, 8010 Graz, Austria.
authors: Philipp
authors: P
authors: Aschauer
authors: Institute of Molecular Biology, University of Graz, Humboldtstrasse 50/3, 8010 Graz, Austria.
authors: Christian
authors: C
authors: Sturm
authors: Institute of Molecular Biology, University of Graz, Humboldtstrasse 50/3, 8010 Graz, Austria.
authors: Monika
authors: M
authors: Oberer
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008994:Monoacylglycerol Lipases / Q000737:chemistry* / Q000302:isolation & purification*; D020033:Protein Isoforms / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D014961:X-Ray Diffraction
chemical_list: D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins; D008994:Monoacylglycerol Lipases; C552818:Yju3 protein, S cerevisiae
keywords: monoacylglycerol lipase; monoglyceride lipase; s-Yju3p