title: An amperometric biosensor for L-glutamate determination prepared from L-glutamate oxidase immobilized in polypyrrole-polyvinylsulphonate film.
abstract: In this paper, a novel amperometric L-glutamate (Glu) biosensor with immobilization of L-glutamate oxidase (L-GlOx) on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been successfully developed. L-GlOx enzyme was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of Glu was carried out by oxidation of enzymatically produced H2O2 at 0.3 V versus Ag/AgCl. The optimum pH and temperature parameters were found to be 9.0 and 55 °C, respectively. There were three linear parts in the regions between 1.0 × 10(-9) and 1.0 × 10(-8) M (R(2) = 0.847), 5.0 × 10(-8) and 5.0 × 10(-7) M (R(2) = 0.997), 5.0 × 10(-7) and 5.0 × 10(-5) M (R(2) = 0.994). Storage stability, operation stability of the enzyme electrode were also studied.
authors: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.
authors: Şule
authors: Ş
authors: Şimşek
authors: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.|b Department of Chemistry , Faculty of Science and Art, Bozok University , Yozgat , Turkey.
authors: Elif
authors: E
authors: Aynacı
authors: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.
authors: Fatma
authors: F
authors: Arslan
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized; D018698:Glutamic Acid / Q000097:blood*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D013451:Sulfonic Acids; C008959:lyapolate; D012710:Serum Albumin, Bovine; C067635:polypyrrole; D018698:Glutamic Acid; D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase; D005976:Glutaral
keywords: L-Glutamate; L-glutamate oxidase; biosensor; polypyrrole; polyvinylsulphonate