title: Bio-mimetic mineralization potential of collagen hydrolysate obtained from chromium tanned leather waste.
abstract: Hydroxyapatite (HA) ceramics serve as an alternative to autogenous-free bone grafting by virtue of their excellent biocompatibility. However, chemically synthesized HA lacks the strong load-bearing capacity as required by bone. The bio-mimetic growth of HA crystals on collagen surface provides a feasible solution for synthesizing bone substitutes with the desired properties. This study deals with the utilization of the collagen hydrolysate recovered from leather waste as a substrate for promoting HA crystal growth. Bio-mimetic growth of HA was induced by subjecting the hydrolysate to various mineralization conditions. Parameters that would have a direct effect on crystal growth were varied to determine the optimal conditions necessary. Maximum mineralization was achieved with a combination of 10mM of CaCl2, 5mM of Na2HPO4, 100mM of NaCl and 0.575% glutaraldehyde at a pH of 7.4. The metal-protein interactions leading to formation of HA were identified through Fourier-transform infrared (FTIR) spectroscopy and x-ray diffraction (XRD) studies. The crystal dimensions were determined to be in the nanoscale range by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The size and crystallinity of bio-mimetically grown HA indicate that hydrolysate from leather waste can be used as an ideal alternative substrate for bone growth.
authors: School of Bio Science and Technology, Vellore Institute of Technology University, Vellore 632014, Tamil Nadu, India.
authors: Pradipta
authors: P
authors: Banerjee
authors: School of Bio Science and Technology, Vellore Institute of Technology University, Vellore 632014, Tamil Nadu, India.
authors: S
authors: S
authors: Madhu
authors: Tannery Division, CSIR-Central Leather Research Institute, Chennai 600 020, Tamil Nadu, India.
authors: N K
authors: NK
authors: Chandra Babu
authors: School of Bio Science and Technology, Vellore Institute of Technology University, Vellore 632014, Tamil Nadu, India. Electronic address: cshanthi@vit.ac.in.
authors: C
authors: C
authors: Shanthi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics / Q000379:methods; D018786:Bone Substitutes / Q000737:chemistry; D016025:Bone Transplantation / Q000379:methods; D001842:Bone and Bones / Q000737:chemistry; D002857:Chromium / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002857:Chromium; D003094:Collagen; D017886:Durapatite
keywords: Bio-mimetic growth; Bone; Collagen hydrolysate; Hydroxyapatite; Leather waste; Mineralization