title: High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine.
abstract: S-adenosyl-l-methionine (AdoMet), an important biological cofactor, exists in two chiral forms, (S,S)- and (R,S)-, only the former of which is biologically active. Here, we have developed a chromatographic method to obtain pure (S,S)-AdoMet using a single C18 column.
authors: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.
authors: Jianyu
authors: J
authors: Zhang
authors: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: klinman@berkeley.edu.
authors: Judith P
authors: JP
authors: Klinman
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012436:S-Adenosylmethionine / Q000302:isolation & purification*
chemical_list: D012436:S-Adenosylmethionine
keywords: Chiral resolution; Diastereoisomer; High-performance liquid chromatography; Radioactive; S-adenosyl-l-methionine; Separation