title: Chitooligomers preparation by chitosanase produced under solid state fermentation using shrimp by-products as substrate.
abstract: Solid state fermentation (SSF) conditions were statistically optimized for the production of chitosanase by Purpureocillium lilacinum CFRNT12 using shrimp by-products as substrate. Central composite design and response surface methodology were applied to evaluate the effect of variables and their optimization. Incubation temperature, incubation time, concentration of inoculum and yeast extract were found to influence the chitosanase production significantly. The R(2) value of 0.94 indicates the aptness of the model. The level of variables for optimal production of chitosanase was 32 ± 1°C temperature, 96 h incubation, 10.5% (w/v) inoculum, 1.05% (w/w) yeast extract and 65% (w/w) moisture content. The chitosanase production was found to increase from 2.34 ± 0.07 to 41.78 ± 0.73 units/g initial dry substrate after optimization. The crude chitosanase produced 4.43 mM of chitooligomers as exclusive end product from colloidal chitosan hydrolysis. These results indicate the potential of P. lilacinum CFRNT12 for the chitosanase production employing cost effective SSF using shrimp by-products.
authors: Academy of Scientific and Innovative Research, Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
authors: T
authors: T
authors: Nidheesh
authors: Academy of Scientific and Innovative Research, Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
authors: Gaurav Kumar
authors: GK
authors: Pal
authors: Academy of Scientific and Innovative Research, Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India. Electronic address: drsureshpv@cftri.res.in.
authors: P V
authors: PV
authors: Suresh
mesh_terms: D000818:Animals; D001709:Biotechnology; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D006999:Hypocreales / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D007220:Industrial Waste; D033561:Penaeidae; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D007220:Industrial Waste; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase
keywords: Chitooligomers; Chitosanase; Purpureocillium lilacinum; Response surface methodology; Shrimp by-products; Solid state fermentation