title: Inflammatory cytokine gene polymorphism profiles in Turkish patients with ulcerative colitis.
abstract: OBJECTIVE
  To investigate IL-1α, IL-1β, IL-1R , IL-4RA, TGF-β, TNF-α and IFN-γ, genes polymorphism in Turkish patients with ucerative colitis (UC).
  
  
  METHODS
  An analysis was carried out at Trabzon Karadeniz Technical University Medicine Faculty Gastroenterology polyclinics between March 2005 and May 2011 on 51 patient with UC (cases) and 100 healthy individuals (controls). PCR-SSP and cytokine gene panel (Helderberg kits) based techniques for analysis of gene polymorphisms were used.
  
  
  RESULTS
  Changes in allelic frequencies of each of the investigated eight cytokine genes polymorphisms in patient with ulcerative colitis were found. Among the allelic genes analyzed here, the highest statistically significant change was observed in the position TNF-α -308 G/A (339.7%). The following increases were observed in IL-IR mspa T/C variation (179.4%), IFN-γ 5644A/T variation (77.4%), and in IL-1β -511T/C SNPs (35.9% ). In other analyzed genes, allelic changes were found to be decreasing for TGF- β codon10C / T (-71.9%), IL4RA + 1902G / A (-47.3 %), and for IL- 1α -889T / C (-37.7%). The lowest negative change (-25.9%) was observed in the allele frequency in IL- 1β 3962T / C (p les than 0.000). In addition, there were changes in genotypic frequencies investigated seven gene polymophic site and only one of cytokine gene IL-1β 3962TT/TC/CC was not changed.
  
  
  CONCLUSIONS
  Genes polymorphism is not itself the only determining factor for clinical diagnoses. However, it can be used in the clinical diagnosis of UC in order to determine the low level or high level variations in cytokine gene polymorphisms.
authors: Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University Kars, Turkey.
authors: İlhami
authors: İ
authors: Gök
authors: Departments of Medical Biology and Genetics, School of Medicine, Akdeniz University, Antalya, Turkey.
authors: Fahri
authors: F
authors: Uçar
authors: Divisions of Gastroenterology, School of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors: Orhan
authors: O
authors: Ozgur
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D014421:Turkey; D055815:Young Adult
chemical_list: D016207:Cytokines; D007378:Interleukins; C439524:TNF protein, human; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma