title: Correlation of melanophore index with a battery of functional genomic stress indicators for measurement of environmental stress in aquatic ecosystem.
abstract: The correlation of primary stress indicator; melanophore index (MI) with set of genomic stress indicators is important for a better understanding of the cellular stress pathway induced by xenobiotics in aquatic species. This study presents a correlation between melanophore index (MI) and genomic stress indicators in Oreochromis mossambicus treated with lead nitrate, phenol and hexachlorocyclohexane (HCH). O. mossambicus was exposed to sub-lethal concentrations of the different LC50 values (96 h) of the tested chemicals at varying exposure periods and the response via genomic stress indicators and scale melanophores were assessed in accordance with standard protocols. Melanophore index decreased significantly (p<0.01) in a time dependent pattern to the tested chemicals. Gene expression showed significant time dependent increase in the expression of heat shock proteins (HSP70 and HSP60). Vitellogenin (Vtg) expression insignificantly altered. Significant increase in the expression of melanin concentrating hormone (MCH) was observed in response to hexachlorocyclohexane (HCH) in the treated fish. The findings demonstrated an inverse relationship between melanophore index and the set of genomic stress indicators.
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India.
authors: Atul P
authors: AP
authors: Daiwile
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India.
authors: Pravin K
authors: PK
authors: Naoghare
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India.
authors: Manisha D
authors: MD
authors: Giripunje
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India.
authors: P D Prasada
authors: PD
authors: Rao
authors: Ultra-tech, Environmental Consultancy and Laboratory, Pune 411038, India.
authors: Tarun K
authors: TK
authors: Ghosh
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India.
authors: Kannan
authors: K
authors: Krishnamurthi
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India; Cell Biology and Genetics unit, Department of Zoology, University of Ibadan, Nigeria.
authors: Chibuisi G
authors: CG
authors: Alimba
authors: Environmental Health Division, CSIR - National Environmental Engineering Research Institute (NEERI), Nagpur 440020, India. Electronic address: ss_devi@neeri.res.in.
authors: Saravanadevi
authors: S
authors: Sivanesan
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics; D017753:Ecosystem; D004777:Environment; D029941:Fish Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D023281:Genomics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007028:Hypothalamic Hormones / Q000235:genetics; D007854:Lead / Q000633:toxicity*; D007928:Lethal Dose 50; D008543:Melanins / Q000235:genetics; D008547:Melanophores / Q000187:drug effects*; D009566:Nitrates / Q000633:toxicity*; D019800:Phenol / Q000633:toxicity*; D010907:Pituitary Hormones / Q000235:genetics; D013312:Stress, Physiological; D017210:Tilapia / Q000235:genetics*; D014819:Vitellogenins / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D018834:Chaperonin 60; D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D007028:Hypothalamic Hormones; D008543:Melanins; D009566:Nitrates; D010907:Pituitary Hormones; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D007854:Lead; D019800:Phenol; D001556:Hexachlorocyclohexane; C019463:melanin-concentrating hormone; C017461:lead nitrate
keywords: Correlation; Functional stress indicators; HSP; MCH; Melanophore index; Vitellogenin