title: Icariin intervenes in cardiac inflammaging through upregulation of SIRT6 enzyme activity and inhibition of the NF-kappa B pathway.
abstract: The aim of the study was to investigate the effect of icariin (ICA) on cardiac aging through its effects on the SIRT6 enzyme and on the NF-κB pathway. Investigating the effect of ICA on the enzymatic activity of histone deacetylase SIRT6 revealed a concentration of 10(-8) mol/L ICA had a maximum activating effect on histone deacetylase SIRT6 enzymatic activity. Western analysis showed that ICA upregulated SIRT6 protein expression and downregulated NF-κB (p65) protein expression in animal tissues and cell models. ICA upregulated the expression of SIRT6 and had an inhibitory effect on NF-κB inflammatory signaling pathways as shown by decreasing mRNA levels of the NF-κB downstream target genes TNF-α, ICAM-1, IL-2, and IL-6. Those effects were mediated directly or indirectly by SIRT6. We provided evidence that inflammaging may involve a novel link between the effects of ICA on SIRT6 (a regulator of aging) and NF-κB (a regulator of inflammation).
authors: Center of Disease Prevention and Treatment, Shanghai Guanghua Hospital of Integrative Traditional Chinese and Western Medicine, Shanghai 200052, China.
authors: Yang
authors: Y
authors: Chen
authors: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors: Tao
authors: T
authors: Sun
authors: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors: Junzhen
authors: J
authors: Wu
authors: Department of Perinatal Medicine, Pregnancy Research Centre and University of Melbourne Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, VIC 3052, Australia.
authors: Bill
authors: B
authors: Kalionis
authors: Medical College, China Three Gorges University, Yichang 443002, China.
authors: Changcheng
authors: C
authors: Zhang
authors: Medical College, China Three Gorges University, Yichang 443002, China.
authors: Ding
authors: D
authors: Yuan
authors: Key Laboratory of Cellular and Molecular Biology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Jianhua
authors: J
authors: Huang
authors: Key Laboratory of Cellular and Molecular Biology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Waijiao
authors: W
authors: Cai
authors: Department of Diseases Prevention and Healthcare, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors: Hong
authors: H
authors: Fang
authors: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors: Shijin
authors: S
authors: Xia
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology; D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D037761:Sirtuins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016328:NF-kappa B; C501860:Sirt6 protein, mouse; D037761:Sirtuins; C056599:icariin