title: Effects of pulsed 2.856 GHz microwave exposure on BM-MSCs isolated from C57BL/6 mice.
abstract: The increasing use of microwave devices over recent years has meant the bioeffects of microwave exposure have been widely investigated and reported. However the exact biological fate of bone marrow MSCs (BM-MSCs) after microwave radiation remains unknown. In this study, the potential cytotoxicity on MSC proliferation, apoptosis, cell cycle, and in vitro differentiation were assayed following 2.856 GHz microwave exposure at a specific absorption rate (SAR) of 4 W/kg. Importantly, our findings indicated no significant changes in cell viability, cell division and apoptosis after microwave treatment. Furthermore, we detected no significant effects on the differentiation ability of these cells in vitro, with the exception of reduction in mRNA expression levels of osteopontin (OPN) and osteocalcin (OCN). These findings suggest that microwave treatment at a SAR of 4 W/kg has undefined adverse effects on BM-MSCs. However, the reduced-expression of proteins related to osteogenic differentiation suggests that microwave can the influence at the mRNA expression genetic level.
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Changzhen
authors: C
authors: Wang
authors: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors: Xiaoyan
authors: X
authors: Wang
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Hongmei
authors: H
authors: Zhou
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Guofu
authors: G
authors: Dong
authors: NO. 281 Hospital of People's Liberation Army, Qinhuangdao, China.
authors: Xue
authors: X
authors: Guan
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Lifeng
authors: L
authors: Wang
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Xinping
authors: X
authors: Xu
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Shuiming
authors: S
authors: Wang
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Peng
authors: P
authors: Chen
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Ruiyun
authors: R
authors: Peng
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Xiangjun
authors: X
authors: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005720:Gamma Rays; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008872:Microwaves; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature
chemical_list: D012333:RNA, Messenger; D015675:Osteocalcin; D053495:Osteopontin