title: Mycobacterium bovis infection in a horse with granulomatous enterocolitis.
abstract: A 2-year-old dappled Percheron horse had a wasting condition that did not respond to antibiotic treatments and ultimately resulted in death. Thickening of the wall of the large colon and enlargement of the mesenteric lymph nodes were observed at postmortem examination, along with the presence of pinpoint whitish foci in the liver. Microscopic examination of affected tissues revealed diffuse chronic granulomatous enterocolitis, granulomatous mesenteric lymphadenitis, and multifocal granulomatous hepatitis. The DNA extracted from paraffin-embedded intestinal and lymph node samples was analyzed using both a polymerase chain reaction (PCR) assay and PCR-restriction endonuclease analysis and demonstrated the presence of Mycobacterium bovis.
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga) jsarrade@fveter.unr.edu.ar.
authors: Javier E
authors: JE
authors: Sarradell
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Julio
authors: J
authors: Alvarez
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Mariana
authors: M
authors: Biscia
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Martin
authors: M
authors: Zumarraga
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Arno
authors: A
authors: Wunschmann
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Anibal G
authors: AG
authors: Armien
authors: Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
authors: Andres M
authors: AM
authors: Perez
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D004760:Enterocolitis / Q000150:complications / Q000662:veterinary; D017809:Fatal Outcome; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006736:Horses; D008198:Lymph Nodes / Q000473:pathology; D008199:Lymphadenitis / Q000150:complications / Q000662:veterinary; D009164:Mycobacterium Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial
keywords: Argentina; Mycobacterium bovis; horse; tuberculosis