title: Protein disulfide isomerase mediates dengue virus entry in association with lipid rafts.
abstract: Dengue virus (DENV) causes a febrile disease, infecting around 50-100 million people annually. The relationship between DENV proteins and host cellular responses during infection is unclear. This study investigated the interaction of host protein disulfide isomerase (PDI) with DENV proteins and role of lipid rafts in viral immunopathogenesis. Host viral protein interactions were studied by co-immunoprecipitation and co-localization. It was found that PDI interacts with DENV nonstructural protein 1 (NS1) intracellularly as well as on the surface in the lipid raft domain. Disruption of this key interaction between PDI and NS1 could be an important therapeutic strategy to block DENV infection.
authors: Immunomodulation Laboratory, Defence Institute of Physiology and Allied Sciences , Delhi, India .
authors: Drishya
authors: D
authors: Diwaker
authors: Kamla P
authors: KP
authors: Mishra
authors: Lilly
authors: L
authors: Ganju
authors: Shashi B
authors: SB
authors: Singh
mesh_terms: D002460:Cell Line; D003716:Dengue Virus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D021962:Membrane Microdomains / Q000378:metabolism*; D009000:Monocytes / Q000821:virology; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: C079340:NS1 protein, Dengue virus type 2; D011991:Receptors, Virus; D017361:Viral Nonstructural Proteins; D019704:Protein Disulfide-Isomerases