title: Daptomycin versus Vancomycin in an Enterococcus faecalis Endophthalmitis Rabbit Model.
abstract: OBJECTIVE
  To compare bactericidal activities of daptomycin (DAP) and vancomycin (VAN) in an experimental rabbit model of Enterococcus faecalis endophthalmitis.
  
  
  METHODS
  The right vitreous cavities of 24 New Zealand rabbits were inoculated with 100 colony-forming units of E. faecalis; and after 24 h, rabbits were randomly divided into three groups. DAP group (n = 8, 0.2 mg/0.05 ml intravitreally), VAN group (n = 8, 1 mg/0.05 ml intravitreally) and balanced salt solution group (BSS, n = 8, 0.05 ml intravitreally). Clinical examination scores were recorded, and vitreous aspirates were obtained for microbiological analysis on days 0, 1, 2, 3 and 4. Rabbits were sacrificed, and the eyes were enucleated for histopathological assessment.
  
  
  RESULTS
  There was no difference between the DAP, VAN and BSS groups in terms of the clinical grading of endophthalmitis 24 h after the inoculation. The bacterial counts were similar between the VAN and DAP groups except on day 1, where it was significantly lower than those in the VAN group (p = 0.003). On day 4, 62% of the eyes treated with DAP, and 50% of the eyes treated with VAN were sterilized. All of the eyes from the BSS group showed increasing bacterial growth from day 0 to day 4. There was no difference between the DAP and VAN groups in terms of the histopathological and clinical examination scores, while they were significantly lower than those in the BSS group.
  
  
  CONCLUSIONS
  This study demonstrates evidence of the effectiveness of DAP for the treatment of experimental E. faecalis endophthalmitis.
authors: a Department of Ophthalmology .
authors: Muammer
authors: M
authors: Ozcimen
authors: b Department of Microbiology and.
authors: Muhammet G
authors: MG
authors: Kurtoglu
authors: a Department of Ophthalmology .
authors: Sertan
authors: S
authors: Goktas
authors: c Department of Pathology , Konya Training and Research Hospital , Konya , Turkey .
authors: Ethem
authors: E
authors: Omeroglu
authors: a Department of Ophthalmology .
authors: Yasar
authors: Y
authors: Sakarya
authors: a Department of Ophthalmology .
authors: Ismail
authors: I
authors: Alpfidan
authors: d Department of Infectious Diseases and Clinical Microbiology , Konya State Hospital , Konya , Turkey .
authors: Serap
authors: S
authors: Ozcımen
authors: a Department of Ophthalmology .
authors: Rabia
authors: R
authors: Sakarya
authors: e Department of Ophthalmology , Konya Private Eye Hospital , Konya , Turkey , and.
authors: Halil I
authors: HI
authors: Yener
authors: f Department of Public Health, Faculty of Medicine , Necmettin Erbakan University , Konya , Turkey.
authors: Lutfi S
authors: LS
authors: Demir
authors: a Department of Ophthalmology .
authors: Faik
authors: F
authors: Saglam
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D017576:Daptomycin / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D009877:Endophthalmitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D015818:Eye Infections, Bacterial / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D058449:Intravitreal Injections; D008826:Microbial Sensitivity Tests; D009883:Ophthalmic Solutions; D011817:Rabbits; D014640:Vancomycin / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000900:Anti-Bacterial Agents; D009883:Ophthalmic Solutions; D014640:Vancomycin; D017576:Daptomycin
keywords: Daptomycin; Enterococcus faecalis; endophthalmitis; rabbit; vancomycin