title: Wheat peptides reduce oxidative stress and inhibit NO production through modulating μ-opioid receptor in a rat NSAID-induced stomach damage model.
abstract: Non-steroidal anti-inflammatory drugs (NSAIDs) induce tissue damage and oxidative stress in animal models of stomach damage. In the present study, the protective effects of wheat peptides were evaluated in a NSAID-induced stomach damage model in rats. Different doses of wheat peptides or distilled water were administered daily by gavage for 30 days before the rat stomach damage model was established by administration of NSAIDs (aspirin and indomethacin) into the digestive tract twice. The treatment of wheat peptides decreased the NSAID-induced gastric epithelial cell degeneration and oxidative stress and NO levels in the rats. Wheat peptides significantly increased the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and decreased iNOS activity in stomach. The mRNA expression level of μ-opioid receptor was significantly decreased in wheat peptides-treated rats than that in in the control rats. The results suggest that NSAID drugs induced stomach damage in rats, wchih can be prevented by wheat peptides. The mechanisms for the protective effects were most likely through reducing NSAID-induced oxidative stress.
authors: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors: Hong
authors: H
authors: Yin
authors: School of Public Health, Ningxia Medical University, Yinchuan 750004, China.
authors: Hui-Zhen
authors: HZ
authors: Cai
authors: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors: Shao-Kang
authors: SK
authors: Wang
authors: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors: Li-Gang
authors: LG
authors: Yang
authors: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. Electronic address: gjsun@seu.edu.cn.
authors: Gui-Ju
authors: GJ
authors: Sun
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology; D001241:Aspirin / Q000009:adverse effects; D005753:Gastric Mucosa / Q000187:drug effects; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000187:drug effects; D007213:Indomethacin / Q000009:adverse effects; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000138:chemical synthesis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010940:Plant Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017450:Receptors, Opioid, mu / Q000187:drug effects*; D013270:Stomach / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000187:drug effects; D014908:Triticum / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D010940:Plant Proteins; D012333:RNA, Messenger; D017450:Receptors, Opioid, mu; D009569:Nitric Oxide; D005979:Glutathione Peroxidase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D001241:Aspirin; D007213:Indomethacin
keywords: Oxidative stress; Stomach damage; Wheat peptides; iNOS; μ-Opioid receptor