title: Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration.
abstract: Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxal-5'-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved.
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Hyun Joong
authors: HJ
authors: Kim
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Yong Hyun
authors: YH
authors: Kim
authors: Department of Biological and Chemical Engineering, Hongik University, Sejong city, Republic of Korea.
authors: Ji-Hyun
authors: JH
authors: Shin
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Shashi Kant
authors: SK
authors: Bhatia
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Ganesan
authors: G
authors: Sathiyanarayanan
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Hyung-Min
authors: HM
authors: Seo
authors: Department of Environmental Engineering, Ajou University, Suwon 443-749, Republic of Korea.
authors: Kwon Young
authors: KY
authors: Choi
authors: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Yung-Hun
authors: YH
authors: Yang
authors: Department of Biological and Chemical Engineering, Hongik University, Sejong city, Republic of Korea.
authors: Kyungmoon
authors: K
authors: Park
mesh_terms: D001711:Biotransformation; D002103:Cadaverine / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D008239:Lysine / Q000378:metabolism*; D060847:Metabolic Engineering; D011732:Pyridoxal Phosphate / Q000378:metabolism
chemical_list: D011732:Pyridoxal Phosphate; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine
keywords: Biotransformation; Escherichia coli; cadaverine; high concentration reaction; lysine decarboxylase; whole cell biocatalyst