title: Genome sequence and description of the mosquitocidal and heavy metal tolerant strain Lysinibacillus sphaericus CBAM5.
abstract: Lysinibacillus sphaericus CBAM5, was isolated from subsurface soil of oil well explorations in the Easter Planes of Colombia. This strain has potential in bioremediation of heavy-metal polluted environments and biological control of Culex quinquefasciatus. According to the phylogenetic analysis of 16S rRNA gene sequences, the strain CBAM5 was assigned to the Lysinibacillus sphaericus taxonomic group 1 that comprises mosquito pathogenic strains. After a combination assembly-integration, alignment and gap-filling steps, we propose a 4,610,292 bp chromosomal scaffold. The whole genome (consisting of 5,146,656 bp long, 60 contigs and 5,209 predicted-coding sequences) revealed strong functional and syntenial similarities to the L. sphaericus C3-41 genome. Mosquitocidal (Mtx), binary (Bin) toxins, cereolysin O, and heavy metal resistance clusters from nik, ars, czc, mnt, ter, cop, cad, and znu operons were identified.
authors: Centro de Investigaciones Microbiológicas - CIMIC, Universidad de los Andes, Bogotá, Colombia.
authors: Tito David
authors: TD
authors: Peña-Montenegro
authors: Centro de Investigaciones Microbiológicas - CIMIC, Universidad de los Andes, Bogotá, Colombia.
authors: Lucía
authors: L
authors: Lozano
authors: Centro de Investigaciones Microbiológicas - CIMIC, Universidad de los Andes, Bogotá, Colombia.
authors: Jenny
authors: J
authors: Dussán
keywords: Binary toxins; DNA homology; Heavy metal tolerance; Lysinibacillus sphaericus CBAM5; Mosquitocidal toxins; S-layer proteins