title: Chemical constituents of Swertia mussotii and their anti-hepatitis B virus activity.
abstract: Three new secoiridoid aglycones of (-)-swermusic acid A (1) and B (3), and (-)-swerimuslatone A (2), and four new secoiridoid glycosides of 6'-O-formylsweroside (4), 6'-O-formylgentiopicroside (5), 6'-O-acetylamarogentin (6) and 6'-O-acetylamaronitidin (7), along with 40 known compounds (8-47) were isolated from Swertia mussotii. Their structures were elucidated on the basis of extensive spectroscopic analyses including MS, IR, UV, 1D- and 2D-NMR. Forty-five compounds from S. mussotii were evaluated for their anti-HBV activity on the HepG 2.2.15 cell line in vitro inhibiting the secretions of HBsAg and HBeAg, as well as HBV DNA replication. Six of the nine phenols 26-29, 31 and 32 exhibited activities inhibiting HBsAg and HBeAg secretion with IC50 values from 0.23 to 5.18mM, and HBV DNA replication with IC50 values from <0.06 to 2.62mM. Moreover, isooriention (45) displayed significant anti-HBV activities against secretions of HBsAg and HBeAg with IC50 value of 0.79 and 1.12mM, as well as HBV DNA replication with IC50 value of 0.02mM.
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors: Tuan-Wu
authors: TW
authors: Cao
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors: Chang-An
authors: CA
authors: Geng
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors: Yun-Bao
authors: YB
authors: Ma
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors: Xue-Mei
authors: XM
authors: Zhang
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors: Jun
authors: J
authors: Zhou
authors: Research Center for Biological Resources in Qinghai-Tibet Plateau, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China.
authors: Yan-Duo
authors: YD
authors: Tao
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China. Electronic address: chenjj@mail.kib.ac.cn.
authors: Ji-Jun
authors: JJ
authors: Chen
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D029967:Swertia / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D039823:Iridoids; D010936:Plant Extracts
keywords: (−)-swerimuslatone A; (−)-swermusic acid A and B; Anti-HBV activity; Chemical constituents; Gentianaceae; Swertia mussotii