title: Inhibitory effect of Erythronium japonicum on the human breast cancer cell metastasis.
abstract: OBJECTIVE
  In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined.
  
  
  METHODS
  Cells were cultured with DMSO or with 50, 75, 100 or 250 µg/ml of Erythronium japonicum methanol or ethanol extract.
  
  
  RESULTS
  Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts.
  
  
  CONCLUSIONS
  Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.
authors: Department of Food & Nutrition / Research Institute of Human Ecology, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonnam 534-729, Korea.
authors: Mi-Kyoung
authors: MK
authors: You
authors: Nature Pure Korea Inc. Jeonnam 517-803, Korea.
authors: Min-Sook
authors: MS
authors: Kim
authors: Department of Food & Nutrition / Research Institute of Human Ecology, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonnam 534-729, Korea.
authors: Jin
authors: J
authors: Rhyu
authors: Jeonnam Biofood Technology Center, Jeonnam 520-330, Korea.
authors: Mi-Ae
authors: MA
authors: Bang
authors: Department of Food & Nutrition / Research Institute of Human Ecology, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonnam 534-729, Korea.
authors: Hyeon-A
authors: HA
authors: Kim
keywords: Erythronium japonicum; apoptosis; cancer; invasion; proliferation