title: High miR-24 expression is associated with risk of relapse and poor survival in acute leukemia.
abstract: MicroRNAs (miRNAs) play an essential role in the development and progression of acute leukemia (AL). miR-24 promotes the survival of hematopoietic cells. However, little is known concerning the function of miR-24 in human AL. The aim of the present study was to investigate the clinical significance of miR-24 expression in AL. miR-24 expression in 147 patients with AL and 100 healthy individuals was measured by quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). The results showed that compared with the healthy individuals, the expression of miR-24 in AL patients was significantly higher (p<0.001). In addition, miR-24 was expressed at significantly higher levels in acute myeloid leukemia (AML) patients and at significantly lower levels in acute lymphoblastic leukemia (ALL) (p<0.001). More importantly, Kaplan-Meier analysis showed that AL patients with high miR-24 expression tended to have shorter overall survival (p<0.05). In the multivariate analysis stratified for known prognostic variables, miR-24 was identified as an independent prognostic marker. Our data indicated that miR-24 upregulation was associated with poor prognosis in AL. miR-24 was identified for the first time as an independent marker for predicting the clinical outcome of AL patients.
authors: Institute of Cellular Physiology, National Autonomous University of Mexico (UNAM), University City, D.F., Mexico.
authors: Jorge
authors: J
authors: Organista-Nava
authors: Research Department, State Cancer Institute, 'Arturo Beltran Ortega', Acapulco, Guerrero, Mexico.
authors: Yazmín
authors: Y
authors: Gómez-Gómez
authors: Laboratory of Molecular Biomedicine, School of Chemical-Biological Sciences, Guerrero State University, Chilpancingo, Guerrero, Mexico.
authors: Berenice
authors: B
authors: Illades-Aguiar
authors: Laboratory of Cytopathology, School of Chemical-Biological Sciences, Guerrero State University, Chilpancingo, Guerrero, Mexico.
authors: Luz
authors: L
authors: Del Carmen Alarcón-Romero
authors: Research Department, State Cancer Institute, 'Arturo Beltran Ortega', Acapulco, Guerrero, Mexico.
authors: Mónica Virginia
authors: MV
authors: Saavedra-Herrera
authors: Research Department, State Cancer Institute, 'Arturo Beltran Ortega', Acapulco, Guerrero, Mexico.
authors: Ana Bertha
authors: AB
authors: Rivera-Ramírez
authors: Research Department, State Cancer Institute, 'Arturo Beltran Ortega', Acapulco, Guerrero, Mexico.
authors: Víctor Hugo
authors: VH
authors: Garzón-Barrientos
authors: Laboratory of Molecular Biomedicine, School of Chemical-Biological Sciences, Guerrero State University, Chilpancingo, Guerrero, Mexico.
authors: Marco Antonio
authors: MA
authors: Leyva-Vázquez
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D053208:Kaplan-Meier Estimate; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D007958:Leukocyte Count; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm