title: Genomic study of the absorption mechanism of p-coumaric acid and caffeic acid of extract of Ananas comosus L. leaves.
abstract: Cardiac disease has emerged as the leading cause of death worldwide, and food rich in phenolic acids has drawn much attention as sources of active substances of hypolipidemic drug. Ananas comosus L. (pineapple) is one of the most popular tropical and subtropical fruits. Isolated from pineapple leaves, EAL(Extract of Ananas Comosus L. Leaves) is rich in phenolic acids, such as p-coumaric acid, caffeic acid, and other phenolics, highly relevant to the putative cardiovascular-protective effects, which suggests its potential to be a new plant medicine for treatment of cardiac disease, but little is known about absorption, distribution, metabolism, and excretion of EAL in animals or human beings. In this study, we employed cDNA microarray, Caco-2 cell lines, and rat intestinal model to explore the absorption behavior of p-coumaric acid and caffeic acid in EAL. The permeation of 2 substances was concentration and time dependent. Results also indicated that monocarboxylic acid transporter was involved in the transepithelial transport of p-coumaric acid and caffeic acid.
authors: Inst. of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193, China.
authors: Yun-jie
authors: YJ
authors: Dang
authors: Chun-yan
authors: CY
authors: Zhu
mesh_terms: D031230:Ananas / Q000737:chemistry*; D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells; D002109:Caffeic Acids / Q000493:pharmacokinetics*; D003373:Coumaric Acids / Q000493:pharmacokinetics*; D023281:Genomics; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D010636:Phenols / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000493:pharmacokinetics*; D018515:Plant Leaves / Q000737:chemistry; D011422:Propionates; D051381:Rats
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D027501:Monocarboxylic Acid Transporters; D010636:Phenols; D010936:Plant Extracts; D011422:Propionates; C495469:p-coumaric acid; C040048:caffeic acid
keywords: Ananas comosus L. leave; DNA microarray; absorption mechanism; caffeic acid; p-coumaric acid