title: Diversities of interaction of murine macrophages with three strains of Candida albicans represented by MyD88, CARD9 gene expressions and ROS, IL-10 and TNF-α secretion.
abstract: OBJECTIVE
  To explore the mechanisms underlying the different responses of macrophages to distinct Candida albicans strains.
  
  
  METHODS
  Bone marrow was collected from mice. Macrophages were independently incubated with 3 Candida albicans strains.
  
  
  RESULTS
  MyD88 expression in Candida albicans 3683 group was significantly higher than that in Candida albicans 3630 group and Candida albicans SC5314 group, and marked difference was also observed between later two groups (P<0.05). CARD9 expression in Candida albicans 3630 group was higher than that in Candida albicans 3683 group and Candida albicans SC5314 group. Fluorescence intensity was 46.78±0.79 in Candida albicans 3630 group, 32.60±1.31 in Candida albicans 3683 group and 19.40±0.58 in Candida albicans SC5314, and significant difference was observed between any two groups (P<0.05). TNF-α and IL-10 were 18.9843±0.7081 pg/ml and 11.6690±0.3167 pg/ml, respectively, in Candida albicans 3683 group, which were markedly higher than those in Candida albicans 3630 group and Candida albicans SC5314 group (P<0.05 and 0.01).
  
  
  CONCLUSIONS
  Different Candida albicans strains may induce CARD9 expression and alter the production of ROS, TNF-α and IL-10 in macrophages, which may be one of mechanisms underlying the different killing effects of macrophages on distinct Candida albicans strains.
authors: Department of Endodontics, School and Hospital of Stomatology, Sun Yat-Sen University Guangzhou 510055, China.
authors: Xiaohuan
authors: X
authors: Zhang
authors: Department of Oral Biology, Guanghua School of Stomatology, Sun Yat-Sen University Guangzhou 510055, China.
authors: Yanping
authors: Y
authors: Ge
authors: Department of Oral Biology, Guanghua School of Stomatology, Sun Yat-Sen University Guangzhou 510055, China.
authors: Wenqing
authors: W
authors: Li
authors: Department of Oral Biology, Guanghua School of Stomatology, Sun Yat-Sen University Guangzhou 510055, China ; Guangdong Provincial Key Laboratory of Stomatology Guangzhou 510055, China.
authors: Yan
authors: Y
authors: Hu
keywords: CARD9; Candida albicans; IL-10/TNF-α; MyD88; macrophage; reactive oxygen species