title: Anti-apoptotic effect of spermatogonial stem cells on doxorubicin-induced testicular toxicity in rats.
abstract: The present study was designed to investigate whether spermatogonial stem cells (SSCs) have possible effect on doxorubicin (DOX)-induced testicular apoptosis and damaged oxidant/antioxidant balance in rats. Sixty male Albino rats were divided into 3 groups: the saline control group, the testicular toxicity group (2mg/kg DOX once a week for 8 weeks) and the third group is a donor stem cells transplanted following pre-treatment with DOX. After the 8th week, the rats were sacrificed and tissues were collected and examined for CD95, CD95L, Caspase 3, and Caspase 8 gene expression using RT-PCR. While malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) were determined using colorimetric kits. Biochemical, histopathological and PCR results showed improvement of the SSCs' group compared to the DOX-group. It was observed that spermatogonial stem cell affected DOX-induced activation of intrinsic apoptotic signaling pathway via preventing DOX-induced increases in CD95 and CD95L levels as well as cleaved Caspase-8 and Caspase-3 levels in testicular tissues, however, spermatogonial stem cell decreased Dox-induced NF-κB activation as well. It can be concluded that SSCs may be utilized to develop new cell-based therapies, and to advance germline gene therapy.
authors: Biochemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt. Electronic address: drhussieny2009@yahoo.com.
authors: Rasha H
authors: RH
authors: Mohamed
authors: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Rehab A
authors: RA
authors: Karam
authors: Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Hoda A
authors: HA
authors: Hagrass
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Mona G
authors: MG
authors: Amer
authors: Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Manal R
authors: MR
authors: Abd El-Haleem
mesh_terms: D053687:Adult Stem Cells / Q000637:transplantation*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis; D053181:Caspase 8 / Q000096:biosynthesis; D002374:Catalase / Q000378:metabolism; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004317:Doxorubicin / Q000494:pharmacology / Q000633:toxicity*; D004789:Enzyme Activation; D053222:Fas Ligand Protein / Q000096:biosynthesis; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D012671:Seminiferous Tubules / Q000502:physiology; D015398:Signal Transduction; D013076:Sperm Count; D013081:Sperm Motility; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects; D019014:fas Receptor / Q000096:biosynthesis
chemical_list: D000903:Antibiotics, Antineoplastic; D053222:Fas Ligand Protein; D016328:NF-kappa B; D019014:fas Receptor; D008315:Malondialdehyde; D004317:Doxorubicin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D053148:Caspase 3; D053181:Caspase 8
keywords: Doxorubicin; Real-time PCR; SSCs; Testicular toxicity