title: MicroRNA-29A/PTEN pathway modulates neurite outgrowth in PC12 cells.
abstract: PTEN serves as an intrinsic brake on neurite outgrowth, but the regulatory mechanism that governs its action is not clear. In the present study, miR-29a was found to increase neurite outgrowth by decreasing PTEN expression. Results showed that miR-92a-1, miR-29a, miR-92b, and miR-29c expression levels increased during nerve growth factor (NGF)-induced differentiation of PC12 cells. Based on in silico analysis of possible miR-29a targets, PTEN mRNA may be a binding site for miR-29a. A protein expression assay and luciferase reporter assay showed that miR-29a could directly target the 3'-UTRs (untranslated regions) of PTEN mRNA and down-regulate the expression of PTEN. PC12 cells infected with lentiviral pLKO-miR-29a showed far higher levels of miR-29a and Akt phosphorylation level than those infected with control. This promoted neurite outgrowth of PC12 cells. Collectively, these results indicate that miR-29a is an important regulator of neurite outgrowth via targeting PTEN and that it may be a promising therapeutic target for neural disease.
authors: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
authors: H
authors: H
authors: Zou
authors: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
authors: Y
authors: Y
authors: Ding
authors: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
authors: K
authors: K
authors: Wang
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: E
authors: E
authors: Xiong
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: W
authors: W
authors: Peng
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: F
authors: F
authors: Du
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Z
authors: Z
authors: Zhang
authors: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China. Electronic address: czljbljb@126.com.
authors: J
authors: J
authors: Liu
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China. Electronic address: ahg5@ujs.edu.cn.
authors: A
authors: A
authors: Gong
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D015153:Blotting, Western; D049108:Cell Enlargement; D005822:Genetic Vectors; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C526366:MIRN29 microRNA, rat; C000592377:MIRN92 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat
keywords: Akt; PTEN; miR-29a; miRNA; neurite outgrowth