title: Small non-coding RNA deregulation in endometrial carcinogenesis.
abstract: Small non-coding RNAs (sncRNAs) represent a heterogeneous group of <200nt-long transcripts comprising microRNAs, PIWI-interacting RNAs (piRNAs) and small-nucleolar-RNAs (snoRNAs) involved in physiological and pathological processes such as carcinogenesis and tumor progression. Aberrant sncRNA expression in cancer has been associated with specific clinical phenotypes, grading, staging, metastases development and resistance to therapy.Aim of the present work is to study the role of sncRNAs in endometrial carcinogenesis. Changes in sncRNA expression were identified by high-throughput genomic analysis of paired normal, hyperplastic and cancerous endometrial tissues obtained by endometrial biopsies (n = 10). Using smallRNA sequencing and microarrays we identified significant differences in sncRNA expression pattern between normal, hyperplastic and neoplastic endometrium. This led to the definition of a sncRNA signature (129 microRNAs, 2 of which not previously described, 10 piRNAs and 3 snoRNAs) of neoplastic transformation. Functional bioinformatics analysis identified as downstream targets multiple signaling pathways potentially involved in the hyperplastic and neoplastic tissue responses, including Wnt/β-catenin, and ERK/MAPK and TGF-β-Signaling.Considering the regulatory role of sncRNAs, this newly identified sncRNA signature is likely to reflect the events leading to endometrial cancer, which can be exploited to dissect the carcinogenic process including novel biomarkers for early and non-invasive diagnosis of these tumors.
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Maria
authors: M
authors: Ravo
authors: Fondazione IRCCS SDN, Napoli, Italy.
authors: Angela
authors: A
authors: Cordella
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Antonio
authors: A
authors: Rinaldi
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Giuseppina
authors: G
authors: Bruno
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.|Genomix4Life Srl, Spin-Off of the Laboratory of Molecular Medicine and Genomics, University of Salerno, Baronissi, Italy.
authors: Elena
authors: E
authors: Alexandrova
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Pasquale
authors: P
authors: Saggese
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Giovanni
authors: G
authors: Nassa
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Giorgio
authors: G
authors: Giurato
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Roberta
authors: R
authors: Tarallo
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.|Genomix4Life Srl, Spin-Off of the Laboratory of Molecular Medicine and Genomics, University of Salerno, Baronissi, Italy.
authors: Giovanna
authors: G
authors: Marchese
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Francesca
authors: F
authors: Rizzo
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
authors: Claudia
authors: C
authors: Stellato
authors: Department of Medicine and Surgery and Division of Gynecology and Obstetrics, "SS. Giovanni di Dio e Ruggi d'Aragona - Schola Medica Salernitana", University of Salerno Hospital, Salerno, Italy.
authors: Rossella
authors: R
authors: Biancardi
authors: Department of Medicine and Surgery and Division of Gynecology and Obstetrics, "SS. Giovanni di Dio e Ruggi d'Aragona - Schola Medica Salernitana", University of Salerno Hospital, Salerno, Italy.
authors: Jacopo
authors: J
authors: Troisi
authors: Department of Gynecology and Obstetrics and Pathophysiology of Human Reproduction, University of Naples "Federico II", Napoli, Italy.
authors: Attilio
authors: A
authors: Di Spiezio Sardo
authors: Department of Gynecology and Obstetrics and Pathophysiology of Human Reproduction, University of Naples "Federico II", Napoli, Italy.
authors: Fulvio
authors: F
authors: Zullo
authors: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.|Division of Molecular Pathology and Medical Genomics, "SS. Giovanni di Dio e Ruggi d'Aragona - Schola Medica Salernitana", University of Salerno Hospital, Salerno, Italy.
authors: Alessandro
authors: A
authors: Weisz
authors: Department of Medicine and Surgery and Division of Gynecology and Obstetrics, "SS. Giovanni di Dio e Ruggi d'Aragona - Schola Medica Salernitana", University of Salerno Hospital, Salerno, Italy.
authors: Maurizio
authors: M
authors: Guida
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D058727:RNA, Small Untranslated / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger; D058727:RNA, Small Untranslated
keywords: endometrial cancer; miRNAs; piRNAs; small non-coding RNA; snoRNAs