title: Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae.
abstract: The ability to rapidly respond to nutrient changes is a fundamental requirement for cell survival. Here, we show that the zinc cluster regulator Znf1 responds to altered nutrient signals following glucose starvation through the direct control of genes involved in non-fermentative metabolism, including those belonged to the central pathways of gluconeogenesis (PCK1, FBP1 and MDH2), glyoxylate shunt (MLS1 and ICL1) and the tricarboxylic acid cycle (ACO1), which is demonstrated by Znf1-binding enrichment at these promoters during the glucose-ethanol shift. Additionally, reduced Pck1 and Fbp1 enzymatic activities correlate well with the data obtained from gene transcription analysis. Cells deleted for ZNF1 also display defective mitochondrial morphology with unclear structures of the inner membrane cristae when grown in ethanol, in agreement with the substantial reduction in the ATP content, suggesting for roles of Znf1 in maintaining mitochondrial morphology and function. Furthermore, Znf1 also plays a role in tolerance to pH and osmotic stress, especially during the oxidative metabolism. Taken together, our results clearly suggest that Znf1 is a critical transcriptional regulator for stress adaptation during non-fermentative growth with some partial overlapping targets with previously reported regulators in Saccharomyces cerevisiae.
authors: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
authors: Pitchya
authors: P
authors: Tangsombatvichit
authors: Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine.
authors: Marta V
authors: MV
authors: Semkiv
authors: Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine Department of Biotechnology and Microbiology, University of Rzeszow, Rzeszow 35-601, Poland.
authors: Andriy A
authors: AA
authors: Sibirny
authors: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors: Laran T
authors: LT
authors: Jensen
authors: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
authors: Khanok
authors: K
authors: Ratanakhanokchai
authors: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand nitnipa.soo@kmutt.ac.th.
authors: Nitnipa
authors: N
authors: Soontorngun
mesh_terms: D000332:Aerobiosis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C000602284:Znf1 protein, S cerevisiae
keywords: ATP content; Saccharomyces cerevisiae; mitochondrial defect; non-fermentative metabolism; stress-responsive gene; transcriptional regulator