title: Tissue protein imaging at 1 μm laser spot diameter for high spatial resolution and high imaging speed using transmission geometry MALDI TOF MS.
abstract: We have achieved protein imaging mass spectrometry capabilities at sub-cellular spatial resolution and at high acquisition speed by integrating a transmission geometry ion source with time of flight mass spectrometry. The transmission geometry principle allowed us to achieve a 1-μm laser spot diameter on target. A minimal raster step size of the instrument was 2.5 μm. Use of 2,5-dihydroxyacetophenone robotically sprayed on top of a tissue sample as a matrix together with additional sample preparation steps resulted in single pixel mass spectra from mouse cerebellum tissue sections having more than 20 peaks in a range 3-22 kDa. Mass spectrometry images were acquired in a standard step raster microprobe mode at 5 pixels/s and in a continuous raster mode at 40 pixels/s.
authors: The Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University, Nashville, TN, 37235, USA.
authors: Andre
authors: A
authors: Zavalin
authors: Junhai
authors: J
authors: Yang
authors: Kevin
authors: K
authors: Hayden
authors: Marvin
authors: M
authors: Vestal
authors: Richard M
authors: RM
authors: Caprioli
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins