title: Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM).
abstract: The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
authors: Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
authors: George E
authors: GE
authors: Cutsail
authors: Department of Biological, Chemical and Physical Sciences, Roosevelt University, Chicago, IL 60605, USA.
authors: Joshua
authors: J
authors: Telser
authors: Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA. Electronic address: bmh@northwestern.edu.
authors: Brian M
authors: BM
authors: Hoffman
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods* / Q000639:trends; D005288:Ferredoxins / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D015394:Molecular Structure; D009591:Nitrogenase / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D013455:Sulfur; D007501:Iron; D009591:Nitrogenase
keywords: Advanced paramagnetic resonance spectroscopy of iron–sulfur proteins; Electron nuclear double resonance (ENDOR); Electron spin echo envelope modulation (ESEEM)