title: Lycopus europaeus: phenolic fingerprint, antioxidant activity and antimicrobial effect on clinical Staphylococcus aureus strains.
abstract: Lycopus europaeus L. leaves water extract (LEL) was subjected to phytochemical analysis, and evaluated for its antibacterial and antioxidant effects. Antibacterial activity testing was performed on Staphylococcus aureus clinical strains from catheter-related and skin infections by broth microdilution test. LEL showed bactericidal activity at concentrations from 2500 to 5000 μg/mL against all, including methicillin resistant and polyresistant nosocomial, strains. Antioxidant activity was examined using DPPH and ABTS (11.3 and 9.8 μg/mL, respectively) and by ferric reducing ability of the plasma method (891 μmol AAE/g dry extract). Phytochemical analysis of LEL was performed by LC-DAD-MS/MS. Ten phenolic compounds were identified; two minor compounds (glucopyranosyl rosmarinic acid and sagerinig acid) have not been described in Lycopus yet. The major compounds, considered to be responsible for biological activities detected in the study, were determined as rosmarinic acid (76 mg/g) and luteolin-7-O-glucuronide (23 mg/g). L. europaeus arises from our study as a promising source of antibacterial agent for topical usage.
authors: a Department of Pharmacognosy and Botany , Faculty of Pharmacy, Comenius University in Bratislava , 832 32 Bratislava , Slovak Republic.
authors: Silvia
authors: S
authors: Fialová
authors: b Faculty of Medicine, Institute of Microbiology, Comenius University and University Hospital in Bratislava , 811 08 Bratislava , Slovak Republic.
authors: Lívia
authors: L
authors: Slobodníková
authors: c Department of Pharmaceutical Analysis and Nuclear Pharmacy , Faculty of Pharmacy, Toxicological and Antidoping Center, Comenius University in Bratislava , 832 32 Bratislava , Slovak Republic.
authors: Lucia
authors: L
authors: Veizerová
authors: a Department of Pharmacognosy and Botany , Faculty of Pharmacy, Comenius University in Bratislava , 832 32 Bratislava , Slovak Republic.
authors: Daniel
authors: D
authors: Grančai
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D031333:Lycopus / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Lycopus; antimicrobial; antioxidant; phenolics