title: Effects of hydroethanolic extracts of Balanites aegyptiaca (L.) Delile (Balanitaceae) on some resistant pathogens bacteria isolated from wounds.
abstract: BACKGROUND
  The bark of Balanites aegyptiaca (L.) Delile (Balanitaceae) is widely used in the Togolese folk medicine for the treatment of wounds, malaria and skin diseases. This study aimed to evaluate the antioxidant properties and the activity of the bark extract of this plant against some resistant bacteria isolated from wounds.
  
  
  METHODS
  The antimicrobial activity was assayed using the NCCLS microdilution while the DPPH free radical scavenging and ferric reducing antioxidant power (FRAP) were used for the antioxidant activity. The susceptibility to conventional antibiotics of bacteria was assessed by an agar disk diffusion method using commercial disk. Phenolics were quantified using spectrophotometric methods.
  
  
  RESULTS
  The hydroethanolic extracts of the bark inhibited in vitro the growth of Pseudomonas aeruginosa and Staphylococcus aureus in a dose-dependent manner. Bacteriostatic effects were observed on 15 (33%) and 21 (47%) strains of Staphylococcus aureus and Pseudomonas aeruginosa respectively. The MICs and MBCs against Staphylococcus aureus strains ranged respectively from 2.5 to 12.5 µg/mL and 2.5 to 50 µg/mL. Concerning Pseudomonas aeruginosa, these values were equal (12.5 µg/mL). Multidrug resistance was 95% against six antibiotics for Pseudomonas aeruginosa and 91% against five antibiotics for Staphylococcus aureus. The DPPH radical scavenging and the FRAP assays yielded weak antioxidant activity, closely linked with the total phenolic contents.
  
  
  CONCLUSIONS
  This study indicated that the bark extract of Balanites aegyptiaca possess bioactive compounds implicated in the free radical scavenging and antibacterial activities, justifying the use of the plant in the traditional medicines.
authors: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires - Université de Lomé, B.P. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Lomé,Togo.
authors: Kokou
authors: K
authors: Anani
authors: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires - Université de Lomé, B.P. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Lomé,Togo. Electronic address: neladjrah@gmail.com.
authors: Yao
authors: Y
authors: Adjrah
authors: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires - Université de Lomé, B.P. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo.
authors: Yaovi
authors: Y
authors: Ameyapoh
authors: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires - Université de Lomé, B.P. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo; Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), Lomé,Togo.
authors: Simplice Damintoti
authors: SD
authors: Karou
authors: Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences - Université de Lomé, BP. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo.
authors: Amegnona
authors: A
authors: Agbonon
authors: Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires - Université de Lomé, B.P. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo.
authors: Comlan
authors: C
authors: de Souza
authors: Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences - Université de Lomé, BP. 1515 Lomé, Togo; Centre de Formation et de Recherche sur les Plantes Médicinales (CERFOPLAM), Université de Lomé, Togo.
authors: Messanvi
authors: M
authors: Gbeassor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D032430:Balanites; D024881:Drug Resistance, Bacterial; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D014947:Wounds and Injuries / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts
keywords: Antimicrobial; Antioxidant; Balanites aegyptiaca; Togo; Wounds