title: Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA.
abstract: Somatic reprogramming to generate induced pluripotent stem cells, or iPSC, is a powerful tool in developmental biology, disease modeling, and regenerative medicine. microRNAs have been shown to regulate many key pathways in iPSC induction. Here we describe a microRNA mimic enhanced somatic reprogramming process starting from mouse embryonic fibroblast isolation to iPSC induction to colony derivation and characterization.
authors: Department of Bioengineering, Department of Pediatrics, University of California San Diego School of Medicine, 9500 Gilman Drive MC 0762, La Jolla, CA, 92093, USA.
authors: Jason
authors: J
authors: Dang
authors: Department of Pediatrics, University of California San Diego School of Medicine, 9500 Gilman Drive MC 0762, La Jolla, CA, 92093, USA. trana@ucsd.edu.
authors: Tariq M
authors: TM
authors: Rana
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects / Q000235:genetics*; D000067470:Cellular Reprogramming Techniques / Q000379:methods; D058732:Embryoid Bodies / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D013724:Teratoma / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: Differentiation; Embryoid body; Induced pluripotent stem cell; MicroRNA; Mouse embryonic fibroblasts; Somatic reprogramming; Teratoma; Transfection