title: Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study.
abstract: To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS).In vivofluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes.Ex vivofluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized byin vivoimaging with an acceptable tumor to background ratio.Ex vivostudies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation.
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Qiong
authors: Q
authors: Zhang
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Fan
authors: F
authors: Wang
authors: Department of Orthopedic Surgery, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Yao-Sen
authors: YS
authors: Wu
authors: Department of Gynecology and Obstetrics, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors: Ke-Ke
authors: KK
authors: Zhang
authors: Department of Orthopedic Surgery, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Yan
authors: Y
authors: Lin
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Xue-Qiong
authors: XQ
authors: Zhu
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Jie-Qiang
authors: JQ
authors: Lv
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Xiao-Sheng
authors: XS
authors: Lu
authors: Department of Orthopedic Surgery, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Xiao-Lei
authors: XL
authors: Zhang
authors: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Yue
authors: Y
authors: Hu
authors: Department of Gynecology and Obstetrics, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors: Yin-Ping
authors: YP
authors: Huang
keywords: CD227 antibody; fluorescence imaging; mucin 1 antibody; near-infrared fluorescence; ovarian cancer