title: Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene.
abstract: Pyrene, a typical polycyclic aromatic hydrocarbon, is a common pollutant in the marine environment. Polycyclic aromatic hydrocarbons initiate cellular detoxification in an exposed organism via the activation of the aryl hydrocarbon receptor (AhR). Subsequent metabolism of these xenobiotics is mainly by the cytochrome P450 enzymes of the phase I detoxification system. Full-length complementary DNA sequences from the pearl oyster Pinctada martensii (pm) encoding AhR and cytochrome P4 were cloned. The P. martensii AhR complementary DNA sequence constitutes an open reading frame that encodes for 848 amino acids. Sequence analysis indicated PmAhR showed high similarity with its homologues of other bivalve species. The cytochrome P(CYP)4 complementary DNA sequence of P. martensii constitutes an open reading frame that encodes for 489 amino acids. Quantitative real-time analysis detected both PmAhR and PmCYP4 messenger RNA expressions in the mantle, gill, hepatapancreas and adductor muscle of P. martensii exposed to pyrene. The highest transcript-band intensities of PmAhR and PmCYP4 were observed in the gill. Temporal expression of PmAhR and PmCYP4 messenger RNAs induction was observed in gills and increased between 3 and 5 days post exposure; then returned to control level. These results suggest that messenger RNAs of PmAhR and PmCYP4 in pearl oysters might be useful parameters for monitoring marine environment pyrene pollution.
authors: College of Environment and Plant Protection, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.|Key Laboratory of Tropical Biological Resources, Ministry of Education, Hainan University, Haikou, 570228, China.
authors: Junqiao
authors: J
authors: Du
authors: College of Agriculture, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.
authors: Chenghong
authors: C
authors: Liao
authors: College of Agriculture, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.
authors: Hailong
authors: H
authors: Zhou
authors: College of Agriculture, Hainan University, Haikou, 570228, China. diaoxip@hainu.edu.cn.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China. diaoxip@hainu.edu.cn.
authors: Xiaoping
authors: X
authors: Diao
authors: College of Agriculture, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.
authors: Yuhu
authors: Y
authors: Li
authors: College of Agriculture, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.
authors: Pengfei
authors: P
authors: Zheng
authors: College of Environment and Plant Protection, Hainan University, Haikou, 570228, China.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China.
authors: Fuqiang
authors: F
authors: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003001:Cloning, Molecular; D042926:Cytochrome P-450 CYP4A / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D008969:Molecular Sequence Data; D049911:Pinctada / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D011721:Pyrenes / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D011721:Pyrenes; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; C030984:pyrene; D042926:Cytochrome P-450 CYP4A
keywords: AhR; CYP4; Pinctada martensii; Pyrene; cDNA cloning; mRNA expression