title: Application of QuEChERS based method for the determination of pesticides in nutraceutical products (Camellia sinensis) by liquid chromatography coupled to triple quadrupole tandem mass spectrometry.
abstract: A QuEChERS (quick, easy, cheap, effective, rugged, and safe) based method has been evaluated and validated for the determination and quantification of approximately 100 LC-amenable pesticides in nutraceutical products obtained from green tea (Camellia sinensis). Extraction was performed with acidified acetonitrile (acetic acid 1% (v/v)), and additional clean-up steps were not necessary. Pesticides determination was achieved using ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS). Total running time was 11 min. Pesticides were quantified using matrix-matched calibration. Recoveries ranged from 70% to 117% and relative standard deviation (RSD) was lower than 20% at concentration levels of 25, 50 and 100 μg/kg for intra-day precision and equal or lower than 25% for inter-day precision. Limits of quantification (LOQ) were equal or lower than 25 μg/kg. The validated method was applied to commercial nutraceutical products, detecting acetamiprid (56 μg/kg) and carbendazim (13 μg/kg) in two samples.
authors: Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, CeiA3, E-04120 Almería, Spain.
authors: Gerardo
authors: G
authors: Martínez-Domínguez
authors: Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, CeiA3, E-04120 Almería, Spain.
authors: Antonio José
authors: AJ
authors: Nieto-García
authors: Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, CeiA3, E-04120 Almería, Spain.
authors: Roberto
authors: R
authors: Romero-González
authors: Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, CeiA3, E-04120 Almería, Spain. Electronic address: agarrido@ual.es.
authors: Antonia Garrido
authors: AG
authors: Frenich
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry* / Q000166:cytology; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013662:Tea / Q000737:chemistry
chemical_list: D010575:Pesticides; D013662:Tea
keywords: Green tea; Nutraceuticals; Pesticides; QuEChERS; Tandem mass spectrometry; Ultra high performance liquid chromatography