title: Poly(lactic-co-glycolic acid) matrix incorporated with nisin as a novel antimicrobial biomaterial.
abstract: The use of poly(lactic-co-glycolic acid) (PLGA) matrix as a biomolecule carrier has been receiving great attention due to its potential therapeutic application. In this context, we investigated the PLGA matrix capacity to incorporate nisin, an antimicrobial peptide capable of inhibiting the growth of Gram-positive bacteria and bacterial spores germination. Nisin-incorporated PLGA matrices were evaluated based on the inhibitory effect against the nisin-bioindicator Lactobacillus sakei. Additionally, the PLGA-nisin matrix stability over an 8-months period was investigated, as well as the nisin release profile. For the incorporation conditions, we observed that a 5 h incorporation time, at 30 °C, with 250 μg/mL nisin solution in PBS buffer pH 4.5, resulted in the highest inhibitory activity of 2.70 logAU/mL. The PLGA-nisin matrix was found to be relatively stable and showed sustained drug delivery, with continuous release of nisin for 2 weeks. Therefore, PLGA-nisin matrix is could be used as a novel antimicrobial delivery system and an alternative to antibiotics incorporated into PLGA matrices.
authors: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo - FCF/USP, Av. Prof. Lineu Prestes, 580, B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
authors: Rafaela Coelho
authors: RC
authors: Correia
authors: Angela Faustino
authors: AF
authors: Jozala
authors: Kelly Fernanda
authors: KF
authors: Martins
authors: Thereza Christina Vessoni
authors: TC
authors: Penna
authors: Eliana Aparecida de Rezende
authors: EA
authors: Duek
authors: Carlota de Oliveira
authors: Cde O
authors: Rangel-Yagui
authors: André Moreni
authors: AM
authors: Lopes
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D019344:Lactic Acid / Q000737:chemistry*; D007778:Lactobacillus / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry; D009561:Nisin / Q000737:chemistry* / Q000494:pharmacology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D009561:Nisin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid