title: Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos.
abstract: Personal care products have been detected in superficial waters, representing an environmental risk to the biota. Some studies indicated that 3-benzophenone (3BP) alters hormones, inducing vitellogenesis and having adverse effects on fish reproduction. Other studies have reported generation of free radicals and changes in antioxidant enzymes. Therefore, the aim of the present study was to test acute exposure to 3BP at concentrations within and beyond that found environmentally to provide important toxicological information regarding this chemical. We evaluated the effect of 3BP on vitellogenin 1 (VTG1) gene expression and the transcription of the enzymes catalase (CAT), superoxide dismutase (SOD) or glutathione peroxidase (GPx), which are involved in cellular redox balance. Zebrafish eluthero-embryos (168hpf) were exposed to 1,10, 100, 1000µg/L 3BP, in addition to a negative control and a 0.1% ethanol control for 48h. The results of our study indicated a positive significant correlation between exposure concentrations and VTG1 expression (r=0.986, p=0.0028) but only 1000µg/L 3BP produced a significant increase from control. Acute exposure showed no significant differences in transcription levels of CAT, SOD or GPx at the tested conditions. Nevertheless, a trend toward increase in GPx expression was observed as a positive significant correlation (r=0.928, p=0.017) was noted.
authors: Facultad de Química, Universidad Nacional Autónoma de México, Sisal, Yucatan, Mexico. Electronic address: grf@unam.mx.
authors: Gabriela
authors: G
authors: Rodríguez-Fuentes
authors: Facultad de Química, Universidad Nacional Autónoma de México, Sisal, Yucatan, Mexico; Instituto Tecnológico de Tizimín, Tizimin, Yucatan, Mexico.
authors: Juan J
authors: JJ
authors: Sandoval-Gío
authors: Benemérita Universidad Autónoma de Puebla, Puebla, Puebla, Mexico.
authors: Anita
authors: A
authors: Arroyo-Silva
authors: Facultad de Química, Universidad Nacional Autónoma de México, Sisal, Yucatan, Mexico.
authors: Elsa
authors: E
authors: Noreña-Barroso
authors: Facultad de Ciencias, Universidad Nacional Autónoma de México, Sisal, Yucatan, Mexico.
authors: Karla S
authors: KS
authors: Escalante-Herrera
authors: Facultad de Ciencias, Universidad Nacional Autónoma de México, Sisal, Yucatan, Mexico.
authors: Francisco
authors: F
authors: Olvera-Espinosa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001577:Benzophenones / Q000032:analysis / Q000633:toxicity*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D013473:Sunscreening Agents / Q000032:analysis / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001577:Benzophenones; D004968:Estrogens, Non-Steroidal; D013473:Sunscreening Agents; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C005290:oxybenzone; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: 3-Benzophenone; Danio rerio; Oxidative stress; UV filters; Vitellogenin