title: Nucleus deformation of SaOs-2 cells on rhombic µ-pillars.
abstract: It has been previously shown that osteosarcoma (SaOs-2) cells respond to micropillared surfaces consisting of poly-L-lactic acid with strong deformation of the cell body and nucleus. Until now, cell nucleus deformation of SaOs-2 cells was only studied by exposing them to square shaped micropillars in an isotropic pattern. Here we report on experiments of the cell nucleus response of such cells to rhombic structures of different topographies generated from a rubbery polymer, namely poly(n-butyacrylate). It is observed that cells orientate themselves perpendicular to the long axis of the rhombi. While their spreading on the surface is not influenced by the opening angle of the structures, rhombic structures with sharper angles induce stronger deformation of the cells and accordingly more elongated nuclei.
authors: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany.
authors: Melanie
authors: M
authors: Eichhorn
authors: Cleo
authors: C
authors: Stannard
authors: Karine
authors: K
authors: Anselme
authors: Jürgen
authors: J
authors: Rühe
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D053144:Cell Nucleus Shape / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D048429:Cell Size; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000179:Acrylates; D011108:Polymers; C498324:poly(n-butyl acrylate)