title: A statistically inferred microRNA network identifies breast cancer target miR-940 as an actin cytoskeleton regulator.
abstract: MiRNAs are key regulators of gene expression. By binding to many genes, they create a complex network of gene co-regulation. Here, using a network-based approach, we identified miRNA hub groups by their close connections and common targets. In one cluster containing three miRNAs, miR-612, miR-661 and miR-940, the annotated functions of the co-regulated genes suggested a role in small GTPase signalling. Although the three members of this cluster targeted the same subset of predicted genes, we showed that their overexpression impacted cell fates differently. miR-661 demonstrated enhanced phosphorylation of myosin II and an increase in cell invasion, indicating a possible oncogenic miRNA. On the contrary, miR-612 and miR-940 inhibit phosphorylation of myosin II and cell invasion. Finally, expression profiling in human breast tissues showed that miR-940 was consistently downregulated in breast cancer tissues.
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Ricky
authors: R
authors: Bhajun
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Laurent
authors: L
authors: Guyon
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Amandine
authors: A
authors: Pitaval
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Eric
authors: E
authors: Sulpice
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Stéphanie
authors: S
authors: Combe
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Patricia
authors: P
authors: Obeid
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Vincent
authors: V
authors: Haguet
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Itebeddine
authors: I
authors: Ghorbel
authors: 1] Center for Computational Biology - CBIO, Mines ParisTech, F-77300 Fontainebleau, France [2] Institut Curie, F-75248 Paris, France [3] INSERM, U900, F-75248 Paris, France.
authors: Christian
authors: C
authors: Lajaunie
authors: 1] Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France [2] CEA, iRTSV-BGE, F-38000 Grenoble, France [3] INSERM, BGE, F-38000 Grenoble, France.
authors: Xavier
authors: X
authors: Gidrol
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C000594732:MIRN940 microRNA, human; D035683:MicroRNAs; D020559:Monomeric GTP-Binding Proteins