title: CRKL overexpression suppresses in vitro proliferation, invasion and migration of murine hepatocarcinoma Hca-P cells.
abstract: The signal adaptor CRK family protein play important roles in cancer cell progression, proliferation, migration and invasion. Previously, we showed that CRK was involved in lymphatic metastatic potential of murine hepatocarcinoma cells. In current work, as a member of CRK family, chicken tumour virus number 10 regulator of kinase-like protein (CRKL) was revealed to be associated with malignant behaviors of Hca-P, a murine HCC cell with lymph node metastatic (LNM) rate of ∼25%. CRKL overexpression in Hca-P by a constructed eukaryotic expression vector of pcDNA3.1/V5-HisB-CRKL significantly ameliorated its malignant biological properties. CCK-8 and soft agar colony formation assays indicated CRKL overexpression significantly inhibits the cell proliferation and colony formation abilities of Hca-P. Additionally, transwell assays indicated that the Hca-P cell migration and invasion capacities were apparently reduced following CRKL overexpression. As Hca-P is an ideal hepatocarcinoma cell model with low (initial) LNM potential, CRKL is shown to act as a potential suppressor and to provide new insight for both the malignant behaviors of hepatocarcinoma cells and lymphatic metastasis mechanism of hepatocarcinoma.
authors: Department of Biochemistry, Dalian Medical University, 116044 Dalian, PR China; Department of Biotechnology, Dalian Medical University, 116044 Dalian, PR China.
authors: Qiuyue
authors: Q
authors: Lin
authors: Department of Biotechnology, Dalian Medical University, 116044 Dalian, PR China.
authors: Ming-Zhong
authors: MZ
authors: Sun
authors: Department of Biotechnology, Dalian Medical University, 116044 Dalian, PR China.
authors: Chunmei
authors: C
authors: Guo
authors: Department of Biochemistry, Dalian Medical University, 116044 Dalian, PR China.
authors: Ji
authors: J
authors: Shi
authors: Department of General Surgery of The Second Hospital, Dalian Medical University, 116021 Dalian, PR China.
authors: Xin
authors: X
authors: Chen
authors: Department of Biochemistry, Dalian Medical University, 116044 Dalian, PR China; Provincial Key Laboratory of Cell and Molecular Biology, Dalian Medical University, 116044 Dalian, PR China. Electronic address: smzlsq@163.com.
authors: Shuqing
authors: S
authors: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D014162:Transfection; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D009687:Nuclear Proteins
keywords: CRKL; Hepatocarcinoma; Invasion; Migration; Proliferation