title: Evaluation of 2,6-dichlorophenolindophenol acetate as a substrate for acetylcholinesterase activity assay.
abstract: Ellman's method is a standard protocol for the determination of cholinesterases activity. Though the method is ready for laboratory purposes, it has some drawbacks as well. In the current article, 2,6-dichloroindophenol acetate is performed as a chromogenic substrate suitable for acetylcholinesterase (AChE) activity examination. Michaelis constant and maximal velocity for 2,6-dichloroindophenol acetate were determined (38.0 µM and 244 pkat) and compared to the values for acetythiocholine (K(m) 0.18 mM; V(max) 5.1 nkat). Docking for 2,6-dichloroindophenol acetate and human AChE was done as well. In conclusion, 2,6-dichloroindophenol acetate seems to be suitable chromogenic substrate for AChE and spectrophotometry and based on this it can be easily performed whenever AChE activity should be tested.
authors: a Faculty of Military Health Sciences , University of Defence , Hradec Kralove , Czech Republic .
authors: Miroslav
authors: M
authors: Pohanka
authors: Ondrej
authors: O
authors: Holas
mesh_terms: D015086:2,6-Dichloroindophenol / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000085:Acetates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D000085:Acetates; D002800:Cholinesterase Inhibitors; D015086:2,6-Dichloroindophenol; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Ellman method; assay; butyrylcholinesterase; chromogen; spectrophotometry; substrate