title: Response of stem cells from different origins to biphasic calcium phosphate bioceramics.
abstract: Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). BCPs have been loaded with stem cells from different origins for bone tissue engineering purposes, but evidence of stem cell behavior on different compositions (various HA/β-TCP ratios) and physical features of BCPs is limited. We compared the adhesion, proliferation, viability and osteogenic potential of human mesenchymal stem cells (MSCs) on granular BCPs with equal HA/β-TCP ratio of diverse particle sizes and on porous blocks which had different chemical compositions. In addition, the osteogenic differentiation of MSCs was compared to adipose-derived (ADSC) and dental pulp (DPSC) stem cells, as well as to pre-osteoblasts on a particulate BCP. MSCs growing on granular BCPs demonstrated increased number as compared to MSCs growing on blocks. Cells proliferated to a greater extent on small granular BCPs, while large granular BCPs and blocks promoted cell differentiation. Surprisingly, the expression of genes involved in osteogenesis was upregulated in MSCs on bioceramics in basal medium which indicates that BCPs may have osteoinductive potential. This was confirmed with the upregulation of osteochondrogenic markers, at different time points, when stem cells from various tissues were grown on the BCP. This study demonstrates that BCPs, depending on their physical features and chemical composition, modulate stem cell behavior, and that stem cells from different origins are inherently distinct in their gene expression profile and can be triggered toward osteochondrogenic fate by BCPs.
authors: Department of Morphology and Genetics, Federal University of São Paulo, São Paulo, Brazil, sonja.e.lobo@gmail.com.
authors: Sonja E
authors: SE
authors: Lobo
authors: Robert
authors: R
authors: Glickman
authors: Wagner N
authors: WN
authors: da Silva
authors: Treena L
authors: TL
authors: Arinzeh
authors: Irina
authors: I
authors: Kerkis
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000293:Adolescent; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; C074950:hydroxyapatite-beta tricalcium phosphate; D017886:Durapatite