title: Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers.
abstract: OBJECTIVE
  The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.
  
  
  BACKGROUND
  Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects.
  
  
  METHODS
  Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 ± 0.05.
  
  
  RESULTS
  Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 ± 0.55 µmol/L in all patients, 1.40 ± 0.57 µmol/L in smokers, and 1.50 ± 0.53 µmol/L in non-smokers.
  
  
  CONCLUSIONS
  The method presented here is sensitive and accurate for seminal plasma malondialdehyde determination. Our results showed a relationship between sperm motility and the malondialdehyde level in all patients and non-smokers. Malondialdehyde may induce poor sperm functionality and negatively affect the fertilization processes (Tab. 1, Fig. 1, Ref. 23).
authors: X
authors: X
authors: Stramova
authors: R
authors: R
authors: Kandar
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards*; D003430:Cross-Sectional Studies; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000209:etiology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis; D012680:Sensitivity and Specificity; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*; D013081:Sperm Motility
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D008315:Malondialdehyde