title: Beta-7 integrin controls enterocyte migration in the small intestine.
abstract: OBJECTIVE
  To hypothesize that beta-7 integrin affects cellular migration of both, lymphocytes and enterocytes.
  
  
  METHODS
  The nucleoside analog BrdU was ip injected in beta-7-deficient mice (C57BL/6-Itgb(tmlcgn)/J) of male gender and age-matched male C57BL/J J mice (wild type) 4, 20, or 40 h before analysis. The total small intestine was isolated, dissected, and used for morphometrical studies. BrdU-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum, jejunum, and ileum of each animal. The outer most BrdU-positive cell (cell(max)) was determined per hemi-crypt, numerically documented, and statistically analysed.
  
  
  RESULTS
  Integrins containing the beta-7-chain were exclusively expressed on leukocytes. In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased. Moreover, the Peyer's patches of beta-7 integrin-deficient mice appeared hypoplastic. In beta-7 integrin-deficient mice the location of cell(max) was found in a higher position than it was the case for the controls. The difference was already detected at 4 h after BrdU application, but significantly increased with time (40 h after BrdU injection) in all small intestinal segments investigated, i.e., duodenum, jejunum, and ileum. Migration of small intestinal enterocytes was different between the experimental groups measured by cell(max) locations.
  
  
  CONCLUSIONS
  The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer.
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Elke
authors: E
authors: Kaemmerer
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Paula
authors: P
authors: Kuhn
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Ursula
authors: U
authors: Schneider
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Thomas
authors: T
authors: Clahsen
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Min Kyung
authors: MK
authors: Jeon
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Christina
authors: C
authors: Klaus
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Julia
authors: J
authors: Andruszkow
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Michael
authors: M
authors: Härer
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Sabine
authors: S
authors: Ernst
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Angela
authors: A
authors: Schippers
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Norbert
authors: N
authors: Wagner
authors: Elke Kaemmerer, Paula Kuhn, Ursula Schneider, Min Kyung Jeon, Christina Klaus, Julia Andruszkow, Michael Härer, Nikolaus Gassler, Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany.
authors: Nikolaus
authors: N
authors: Gassler
mesh_terms: D000818:Animals; D002465:Cell Movement; D020895:Enterocytes / Q000378:metabolism*; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D039641:Integrin beta Chains; C091368:integrin beta7
keywords: Barrier function; Cell migration; Inflammatory bowel disease; Integrin; Intercellular junctions