title: Graphene-gold nanoparticle composite: application as a good scaffold for construction of glucose oxidase biosensor.
abstract: In the present work we report a facile method for fabrication of glucose oxidase immobilized on the partially reduced graphene-gold nanocomposite (PRGO-AuNPs/GOx) as a novel biosensor for determination of glucose concentration. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to study the morphology of PRGO and PRGO-AuNPs. Also, fast Fourier transformation infrared spectroscopy (FTIR) and UV-Vis spectroscopy were used to confirm formation of graphene and graphene-gold composite. Then, the electrochemical behavior of PRGO-AuNPs/GOx modified electrode was studied by cyclic voltammetry (CV). Our electrochemical studies, especially chronoamperometry (CA), showed that the PRGO-AuNPs/GOx modified electrode has excellent electrocatalytic activity towards the glucose. The limit of detection and sensitivity towards glucose were estimated as 0.06μM and 15.04mAmM(-1), respectively.
authors: Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
authors: Sina
authors: S
authors: Sabury
authors: Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 45137-66731, Iran. Electronic address: habibkazemi@iasbs.ac.ir.
authors: Sayed Habib
authors: SH
authors: Kazemi
authors: Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
authors: Farhad
authors: F
authors: Sharif
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004566:Electrodes; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Glucose oxidase; Gold nanoparticles; Reduced graphene