title: Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin.
abstract: The C-terminal region of Clostridium perfringens enterotoxin (C-CPE) can bind to specific claudins, resulting in the disintegration of tight junctions (TJs) and an increase in the paracellular permeability across epithelial cell sheets. Here we present the structure of mammalian claudin-19 in complex with C-CPE at 3.7 Å resolution. The structure shows that C-CPE forms extensive hydrophobic and hydrophilic interactions with the two extracellular segments of claudin-19. The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ strands. The helix displacement may thus underlie C-CPE-mediated disassembly of TJs.
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Department of Basic Medical Science, Graduate School of Pharmaceutical Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors: Yasunori
authors: Y
authors: Saitoh
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors: Hiroshi
authors: H
authors: Suzuki
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors: Kazutoshi
authors: K
authors: Tani
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors: Kouki
authors: K
authors: Nishikawa
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Department of Basic Medical Science, Graduate School of Pharmaceutical Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors: Katsumasa
authors: K
authors: Irie
authors: Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Yuki
authors: Y
authors: Ogura
authors: Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Atsushi
authors: A
authors: Tamura
authors: Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Sachiko
authors: S
authors: Tsukita
authors: Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Department of Basic Medical Science, Graduate School of Pharmaceutical Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan. yoshi@cespi.nagoya-u.ac.jp.
authors: Yoshinori
authors: Y
authors: Fujiyoshi
mesh_terms: D000818:Animals; D057167:Claudins / Q000737:chemistry*; D004768:Enterotoxins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D017433:Protein Structure, Secondary; D019108:Tight Junctions / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D057167:Claudins; C502666:Cldn19 protein, mouse; D004768:Enterotoxins; C035598:enterotoxin, Clostridium