title: New Insights on the terpenome of the red seaweed Laurencia dendroidea (Florideophyceae, Rhodophyta).
abstract: The red seaweeds belonging to the genus Laurencia are well known as halogenated secondary metabolites producers, mainly terpenoids and acetogennins. Several of these chemicals exhibit important ecological roles and biotechnological applications. However, knowledge regarding the genes involved in the biosynthesis of these compounds is still very limited. We detected 20 different genes involved in the biosynthesis of terpenoid precursors, and 21 different genes coding for terpene synthases that are responsible for the chemical modifications of the terpenoid precursors, resulting in a high diversity of carbon chemical skeletons. In addition, we demonstrate through molecular and cytochemical approaches the occurrence of the mevalonate pathway involved in the biosynthesis of terpenes in L. dendroidea. This is the first report on terpene synthase genes in seaweeds, enabling further studies on possible heterologous biosynthesis of terpenes from L. dendroidea exhibiting ecological or biotechnological interest.
authors: Institute of Biology, Federal University of Rio de Janeiro (UFRJ) Av. Carlos Chagas Filho, 373-CCS-IB-BLOCO A (ANEXO) A3-202, SAGE-COPPE, Rio de Janeiro 21941-599, Brazil. louisioliveira@gmail.com.
authors: Louisi Souza
authors: LS
authors: de Oliveira
authors: Institute of Biology, Federal University of Rio de Janeiro (UFRJ) Av. Carlos Chagas Filho, 373-CCS-IB-BLOCO A (ANEXO) A3-202, SAGE-COPPE, Rio de Janeiro 21941-599, Brazil. diogoat@gmail.com.
authors: Diogo Antonio
authors: DA
authors: Tschoeke
authors: Federal Institute of Education, Science and Technology of Rio de Janeiro (IFRJ), Campus Duque de Caxias, Avenida República do Paraguai, 120, Sarapuí, Duque de Caxias 25050-100, Brazil. linesoliveira@gmail.com.
authors: Aline Santos
authors: AS
authors: de Oliveira
authors: Research Institute of the Botanical Garden of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, Brazil. lilianhill.bio@gmail.com.
authors: Lilian Jorge
authors: LJ
authors: Hill
authors: Research Institute of the Botanical Garden of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, Brazil. wladimirparadas@gmail.com.
authors: Wladimir Costa
authors: WC
authors: Paradas
authors: Research Institute of the Botanical Garden of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, Brazil. lsalgado.jbrj@gmail.com.
authors: Leonardo Tavares
authors: LT
authors: Salgado
authors: Institute of Biology, Federal University of Rio de Janeiro (UFRJ) Av. Carlos Chagas Filho, 373-CCS-IB-BLOCO A (ANEXO) A3-202, SAGE-COPPE, Rio de Janeiro 21941-599, Brazil. thompsoncristiane@gmail.com.
authors: Cristiane Carneiro
authors: CC
authors: Thompson
authors: Departament of Marine Biology, Federal Fluminense University (UFF), Morro do Valonguinho, s/n, Centro, Niterói 24001-970, Brazil. rcrespo@id.uff.br.
authors: Renato Crespo
authors: RC
authors: Pereira
authors: Institute of Biology, Federal University of Rio de Janeiro (UFRJ) Av. Carlos Chagas Filho, 373-CCS-IB-BLOCO A (ANEXO) A3-202, SAGE-COPPE, Rio de Janeiro 21941-599, Brazil. fabianothompson1@gmail.com.
authors: Fabiano L
authors: FL
authors: Thompson
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D002236:Carbohydrate Conformation; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D044722:Laurencia / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D008798:Mevalonic Acid / Q000378:metabolism; D008958:Models, Molecular; D013729:Terpenes / Q000737:chemistry* / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase; D008798:Mevalonic Acid