title: Dynamic expression and localization of c-MET isoforms in the developing rat pancreas.
abstract: Pancreata from Sprague Dawley rats of different developmental stages were studied to determine the expression and cellular localization of different c-MET isoforms in the developing rat pancreas. Pancreatic mRNA and protein expression levels of c-MET at different developmental stages from embryo to adult were detected by reverse transcription-polymerase chain reaction and by western blotting. To identify the cellular localization of c-MET protein in the developing rat pancreas, double immunofluorescent staining was performed using antibodies for cell type-specific markers and for c-MET. The expression of two isoforms of c-MET (190 kDa and 170 kDa) coincided with the development of the pancreas. The 190 kDa isoform of c-MET is expressed during embryonic stages, and its expression is replaced by the expression of the 170 kDa isoform as the pancreas develops. Only the 170 kDa isoform is expressed in the adult rat pancreas. Throughout all stages of pancreatic development, c-MET is expressed by vimentin-positive cells. In contrast, c-MET staining was stronger in rat pancreata from newborn to adult stages and overlapped with insulin-positive beta-cells. The dynamic expression and localization of different c-MET isoforms in the rat pancreas during different developmental stages indicates that distinct c-MET isoform might be involved in different aspects of pancreatic development.
authors: Key Lab of Antibody Technique of Health Ministry, Nanjing Medical University Nanjing, Jiangsu, P.R. China ; School of Basic Medical Science, Binzhou Medical University Binzhou, Shandong, P.R. China.
authors: Yulong
authors: Y
authors: Wu
authors: School of Nursing, Binzhou Medical University Binzhou, Shandong, P.R. China.
authors: Mei
authors: M
authors: Cheng
authors: School of Basic Medical Science, Binzhou Medical University Binzhou, Shandong, P.R. China.
authors: Zhen
authors: Z
authors: Shi
authors: Key Lab of Antibody Technique of Health Ministry, Nanjing Medical University Nanjing, Jiangsu, P.R. China.
authors: Zhenqing
authors: Z
authors: Feng
authors: Key Lab of Antibody Technique of Health Ministry, Nanjing Medical University Nanjing, Jiangsu, P.R. China.
authors: Xiaohong
authors: X
authors: Guan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D010179:Pancreas / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D020033:Protein Isoforms; D019859:Proto-Oncogene Proteins c-met
keywords: c-MET; development; isoform; pancreas