title: Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient.
abstract: Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5' end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5' end of human Igλ.
authors: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors: Natalia T
authors: NT
authors: Freund
authors: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors: Johannes F
authors: JF
authors: Scheid
authors: Laboratory of Humoral Response to Pathogens, Department of Immunology, Institut Pasteur, Paris 75015, France.
authors: Hugo
authors: H
authors: Mouquet
authors: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA. Electronic address: nussen@rockefeller.edu.
authors: Michel C
authors: MC
authors: Nussenzweig
mesh_terms: D005434:Flow Cytometry; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics*
chemical_list: D007146:Immunoglobulin lambda-Chains
keywords: Anti-HIV-1 antibodies; Human Ig lambda; PCR; Single cell sorting; Somatic hypermutation