title: Chronic intermittent hypoxia increases rat sternohyoid muscle NADPH oxidase expression with attendant modest oxidative stress.
abstract: Chronic intermittent hypoxia (CIH) causes upper airway muscle dysfunction. We hypothesized that the superoxide generating NADPH oxidase (NOX) is upregulated in CIH-exposed muscle causing oxidative stress. Adult male Wistar rats were exposed to intermittent hypoxia (5% O2 at the nadir for 90 s followed by 210 s of normoxia), for 8 h per day for 14 days. The effect of CIH exposure on the expression of NOX subunits, total myosin and 4-hydroxynonenal (4-HNE) protein adducts in sternohyoid muscle was determined by western blotting and densitometry. Sternohyoid protein free thiol and carbonyl group contents were determined by 1D electrophoresis using specific fluorophore probes. Aconitase and glutathione reductase activities were measured as indices of oxidative stress. HIF-1α content and key oxidative and glycolytic enzyme activities were determined. Contractile properties of sternohyoid muscle were determined ex vivo in the absence and presence of apocynin (putative NOX inhibitor). We observed an increase in NOX 2 and p47 phox expression in CIH-exposed sternohyoid muscle with decreased aconitase and glutathione reductase activities. There was no evidence, however, of increased lipid peroxidation or protein oxidation in CIH-exposed muscle. CIH exposure did not affect sternohyoid HIF-1α content or aldolase, lactate dehydrogenase, or glyceraldehyde-3-phosphate dehydrogenase activities. Citrate synthase activity was also unaffected by CIH exposure. Apocynin significantly increased sternohyoid force and power. We conclude that CIH exposure upregulates NOX expression in rat sternohyoid muscle with concomitant modest oxidative stress but it does not result in a HIF-1α-dependent increase in glycolytic enzyme activity. Constitutive NOX activity decreases sternohyoid force and power. Our results implicate NOX-dependent reactive oxygen species in CIH-induced upper airway muscle dysfunction which likely relates to redox modulation of key regulatory proteins in excitation-contraction coupling.
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Robert
authors: R
authors: Williams
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Paul
authors: P
authors: Lemaire
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Philip
authors: P
authors: Lewis
authors: School of Medicine and Medical Science, University College Dublin Dublin, Ireland.
authors: Fiona B
authors: FB
authors: McDonald
authors: School of Medicine and Medical Science, University College Dublin Dublin, Ireland.
authors: Eric
authors: E
authors: Lucking
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Sean
authors: S
authors: Hogan
authors: School of Biochemistry and Cell Biology, University College Cork Cork, Ireland.
authors: David
authors: D
authors: Sheehan
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Vincent
authors: V
authors: Healy
authors: Department of Physiology, School of Medicine, University College Cork Cork, Ireland.
authors: Ken D
authors: KD
authors: O'Halloran
keywords: NADPH oxidase; apocynin; intermittent hypoxia; oxidative stress; respiratory muscle; sleep apnea; sternohyoid; upper airway