title: OY-TES-1 may regulate the malignant behavior of liver cancer via NANOG, CD9, CCND2 and CDCA3: a bioinformatic analysis combine with RNAi and oligonucleotide microarray.
abstract: Given its tumor-specific expression, including liver cancer, OY-TES-1 is a potential molecular marker for the diagnosis and immunotherapy of liver cancers. However, investigations of the mechanisms and the role of OY-TES-1 in liver cancer are rare. In the present study, based on a comprehensive bioinformatic analysis combined with RNA interference (RNAi) and oligonucleotide microarray, we report for the first time that downregulation of OY-TES-1 resulted in significant changes in expression of NANOG, CD9, CCND2 and CDCA3 in the liver cancer cell line BEL-7404. NANOG, CD9, CCND2 and CDCA3 may be involved in cell proliferation, migration, invasion and apoptosis, yet also may be functionally related to each other and OY-TES-1. Among these molecules, we identified that NANOG, containing a Kazal-2 binding motif and homeobox, may be the most likely candidate protein interacting with OY-TES-1 in liver cancer. Thus, the present study may provide important information for further investigation of the roles of OY-TES-1 in liver cancer.
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Qiping
authors: Q
authors: Hu
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Jun
authors: J
authors: Fu
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Bin
authors: B
authors: Luo
authors: Department of Radiology, Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Miao
authors: M
authors: Huang
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Wenwen
authors: W
authors: Guo
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Yongda
authors: Y
authors: Lin
authors: Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Xiaoxun
authors: X
authors: Xie
authors: Department of Neurosurgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
authors: Shaowen
authors: S
authors: Xiao
mesh_terms: D020816:Amino Acid Motifs; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D002455:Cell Division; D002465:Cell Movement; D019295:Computational Biology; D056742:Cyclin D2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D030541:Databases, Genetic; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D000071317:Nanog Homeobox Protein; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D012329:RNA, Bacterial; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D060245:Tetraspanin 29 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D059467:Transcriptome
chemical_list: C485651:ACRBP protein, human; C080336:CCND2 protein, human; C554869:CD9 protein, human; C473796:CDCA3 protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056742:Cyclin D2; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D009363:Neoplasm Proteins; C042027:RNA I; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D060245:Tetraspanin 29