title: Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur.
abstract: We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells.
authors: Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus Givat Ram, Jerusalem 91904, Israel aharon.oren@mail.huji.ac.il.
authors: Aharon
authors: A
authors: Oren
authors: Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus Givat Ram, Jerusalem 91904, Israel.
authors: Lily
authors: L
authors: Mana
authors: Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University in Prague, 12843 Prague, Czech Republic.
authors: Jan
authors: J
authors: Jehlička
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002841:Chromatiaceae / Q000737:chemistry* / Q000648:ultrastructure; D059010:Single-Cell Analysis / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013440:Sulfides / Q000378:metabolism; D013455:Sulfur / Q000032:analysis*
chemical_list: D013440:Sulfides; D002338:Carotenoids; D013455:Sulfur
keywords: Allochromatium vinosum; Allochromatium warmingii; Raman spectroscopy; carotenoids; purple sulfur bacteria; sulfur