title: Multilayered Nanoparticles for Gene Delivery Used to Reprogram Human Foreskin Fibroblasts to Neurospheres.
abstract: Polycationic nanocomplexes are a robust means for achieving nucleic acid condensation and efficient intracellular gene deliveries. To enhance delivery, a multilayered nanoparticle consisting of a core of electrostatically bound elements was used. These included a histone-mimetic peptides, poly-l-arginine and poly-d-glutamic acid was coated with silicate before surface functionalization with poly-l-arginine. Transfection efficiencies and duration of expression were similar when using green fluorescent protein (GFP) plasmid DNA (pDNA) or GFP mRNA. These nanoparticles demonstrated significantly higher (>100%) and significantly longer (15 vs. 4 days) transfection efficiencies in comparison to a commercial transfection agent (Lipofectamine 2000). Reprogramming of human foreskin fibroblasts using mRNA to the Sox2 transcription factor resulted in three-fold higher neurosphere formation in comparison to the commercial reagent. These results demonstrate the potential of these nanoparticles as ideal vectors for gene delivery.
authors: 1 Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York.
authors: Vaibhav
authors: V
authors: Pandit
authors: 2 Ligandal, Inc. , Oakland, California.
authors: Andre
authors: A
authors: Watson
authors: 3 Department of Material Science and Engineering, Rensselaer Polytechnic Institute , Troy, New York.
authors: Liyun
authors: L
authors: Ren
authors: 1 Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York.
authors: Amanda
authors: A
authors: Mixon
authors: 1 Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York.
authors: Shiva P
authors: SP
authors: Kotha
mesh_terms: D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism
chemical_list: D010455:Peptides; C015462:polyarginine