title: MicroRNA expression signature of oral squamous cell carcinoma: functional role of microRNA-26a/b in the modulation of novel cancer pathways.
abstract: BACKGROUND
  MicroRNAs (miRNAs) have been shown to play major roles in carcinogenesis in a variety of cancers. The aim of this study was to determine the miRNA expression signature of oral squamous cell carcinoma (OSCC) and to investigate the functional roles of miR-26a and miR-26b in OSCC cells.
  
  
  METHODS
  An OSCC miRNA signature was constructed by PCR-based array methods. Functional studies of differentially expressed miRNAs were performed to investigate cell proliferation, migration, and invasion in OSCC cells. In silico database and genome-wide gene expression analyses were performed to identify molecular targets and pathways mediated by miR-26a/b.
  
  
  RESULTS
  miR-26a and miR-26b were significantly downregulated in OSCC. Restoration of both miR-26a and miR-26b in cancer cell lines revealed that these miRNAs significantly inhibited cancer cell migration and invasion. Our data demonstrated that the novel transmembrane TMEM184B gene was a direct target of miR-26a/b regulation. Silencing of TMEM184B inhibited cancer cell migration and invasion, and regulated the actin cytoskeleton-pathway related genes.
  
  
  CONCLUSIONS
  Loss of tumour-suppressive miR-26a/b enhanced cancer cell migration and invasion in OSCC through direct regulation of TMEM184B. Our data describing pathways regulated by tumour-suppressive miR-26a/b provide new insights into the potential mechanisms of OSCC oncogenesis and metastasis.
authors: 1] Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan [2] Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: I
authors: I
authors: Fukumoto
authors: Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: T
authors: T
authors: Hanazawa
authors: 1] Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan [2] Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: T
authors: T
authors: Kinoshita
authors: Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: N
authors: N
authors: Kikkawa
authors: 1] Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan [2] Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: K
authors: K
authors: Koshizuka
authors: Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: Y
authors: Y
authors: Goto
authors: Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: R
authors: R
authors: Nishikawa
authors: Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
authors: T
authors: T
authors: Chiyomaru
authors: Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
authors: H
authors: H
authors: Enokida
authors: Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
authors: M
authors: M
authors: Nakagawa
authors: Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: Y
authors: Y
authors: Okamoto
authors: Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan.
authors: N
authors: N
authors: Seki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D015398:Signal Transduction
chemical_list: D008565:Membrane Proteins; D035683:MicroRNAs; C528163:MIRN26 microRNA, human; C000597367:TMEM184B protein, human