title: Evaluation of antimicrobial peptides and cytokine production in primary keratinocyte cell culture from healthy and atopic beagles.
abstract: Increased secretion of antimicrobial peptides and cytokines is present in atopic skin. The purpose of this study was to compare the production of β-defensin (cBD)3-like, cathelicidin (cCath) and cytokines in atopic and healthy canine keratinocytes. Seven atopic house dust mites (HDMs) sensitive and five healthy age-matched beagles were used. Keratinocytes were stimulated for 24 h, and the supernatant was collected. A significantly higher production of cBD3-like was present at baseline in atopic compared with healthy keratinocytes, but cBD3-like did not increase after stimulation. IL-17 and lipopolysaccharide increased cBD3-like in healthy compared with atopic keratinocytes. cCath increased in both groups after stimulation. Atopic keratinocytes exposed to HDM produced more IL-8 and keratinocyte-derived chemokine-like than healthy keratinocytes. Exposure to HDM induced an increased IL-8 in atopic keratinocytes and a decreased IFN-γ in healthy keratinocytes. These results may suggest an over sensitization of atopic keratinocytes and a possible impairment of the cutaneous defense against micro-organisms.
authors: Department of Small Animal Clinical Sciences, University of Florida, Gainesville, FL, USA; Department of Dermatology, College of Medicine, University of Florida, Gainesville, FL, USA.
authors: Domenico
authors: D
authors: Santoro
authors: Kim
authors: K
authors: Ahrens
authors: Rosanna
authors: R
authors: Marsella
authors: Mariangela
authors: M
authors: Segre
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology* / Q000378:metabolism*; D004285:Dogs; D015603:Keratinocytes / Q000276:immunology* / Q000378:metabolism*; D039981:Pyroglyphidae / Q000276:immunology
chemical_list: D023181:Antimicrobial Cationic Peptides; D016207:Cytokines
keywords: antimicrobial peptides and cytokines; atopic dermatitis; keratinocyte