title: Channel characteristics of VDAC-3 from Arabidopsis thaliana.
abstract: Four different isoforms of the Voltage-Dependent Anion Channel (VDAC) have been identified in Arabidopsis plant cells. The electrophysiological characteristics of several VDAC channels from animal as well as plant cells are well documented, but those of this model plant are unknown. One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured. An electrophysiological study of the purified proteins in planar lipid bilayers showed that both methods yielded proteins with similar channel activity. The characteristics of AtVDAC-3 are that of a bona fide VDAC-like channel.
authors: Neuroscience Paris-Saclay Institute (Neuro-PSI) UMR 9197, CNRS-Université Paris-Sud, Bâtiment 430, F-91405 Orsay Cedex, France.
authors: C
authors: C
authors: Berrier
authors: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Sciences Plant, Saclay, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
authors: R
authors: R
authors: Peyronnet
authors: Unité de Microbiologie Structurale & CNRS UMR3528, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors: J-M
authors: JM
authors: Betton
authors: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Sciences Plant, Saclay, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
authors: G
authors: G
authors: Ephritikhine
authors: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Sciences Plant, Saclay, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
authors: H
authors: H
authors: Barbier-Brygoo
authors: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Sciences Plant, Saclay, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
authors: J-M
authors: JM
authors: Frachisse
authors: Neuroscience Paris-Saclay Institute (Neuro-PSI) UMR 9197, CNRS-Université Paris-Sud, Bâtiment 430, F-91405 Orsay Cedex, France. Electronic address: alexandre.ghazi@u-psud.fr.
authors: A
authors: A
authors: Ghazi
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002474:Cell-Free System; D055724:Electrophysiological Phenomena; D004926:Escherichia coli / Q000235:genetics; D008051:Lipid Bilayers; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008051:Lipid Bilayers; D011994:Recombinant Proteins; C000591479:VDAC3 protein, Arabidopsis; D050994:Voltage-Dependent Anion Channels
keywords: Arabidopsis; Cell-free synthesis; Channel; VDAC