title: Polydatin attenuates ipopolysaccharide-induced acute lung injury in rats.
abstract: Anti-inflammatory and anti-apoptotic effects of polydatin (PD) have been demonstrated in our previous studies. Recently, we have found that PD treatment can ameliorate burn-induced acute lung injury (ALI). In the present study, we hypothesized that PD may provide protective effect against LPS-induced ALI through reducing inflammation and apoptosis. Rats were respectively pretreated with PD at doses of 15, 30 and 45 mg/kg weight, followed by intratracheal administration of lipopolysaccharide (LPS). LPS-challenged rats exhibited significant lung injury characterized by the deterioration of histopathology, pulmonary microvascular hyperpermeability, wet-to-dry weight ratio, and oxygenation index, which was attenuated by PD (30 and 45 mg/kg) treatment. Moreover, PD (30 and 45 mg/kg) treatment inhibited LPS-induced inflammatory response, as evidenced by the downregulation of lung myeloperoxidase activity, total cells and PMNs in bronchoalveolar lavage fluid, and the systemic levels of the pro-inflammatory cytokines. Furthermore, PD (30 and 45 mg/kg) treatment remarkably improved LPS-induced increase in TUNEL (deoxynucleotidyl transferase dUTP nick end labeling) staining-positive cells, caspase 3 activity, Bax over-expression and Bcl-2 down-expression. In conclusion, these results demonstrate that PD (30 and 45 mg/kg) treatment attenuates LPS-induced ALI through reducing lung inflammation and apoptosis.
authors: Department of Critical Care Medicine, The First People's Hospital of Chenzhou, Institute of Translation Medicine Chenzou 423000, Hunan Province, China.
authors: Tao
authors: T
authors: Li
authors: Department of Anesthesiology, The University of Hongkong Shenzhen Hospital Shenzhen 518053, China.
authors: Youtan
authors: Y
authors: Liu
authors: Department of Critical Care Medicine, The First People's Hospital of Chenzhou, Institute of Translation Medicine Chenzou 423000, Hunan Province, China ; Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, China.
authors: Guicheng
authors: G
authors: Li
authors: Department of Critical Care Medicine, The First People's Hospital of Chenzhou, Institute of Translation Medicine Chenzou 423000, Hunan Province, China.
authors: Xiang
authors: X
authors: Wang
authors: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, China ; Department of Pathophysiology, Southern Medical University, Guangdong Key Lab of Shock and Microcirculation Research Guangzhou 510515, Guangdong Province, China.
authors: Zhenhua
authors: Z
authors: Zeng
authors: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, China.
authors: Shumin
authors: S
authors: Cai
authors: Department of Critical Care Medicine, The First People's Hospital of Chenzhou, Institute of Translation Medicine Chenzou 423000, Hunan Province, China.
authors: Fengyun
authors: F
authors: Li
authors: Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, China.
authors: Zhongqing
authors: Z
authors: Chen
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D005960:Glucosides; D008070:Lipopolysaccharides; D013267:Stilbenes; C058229:polydatin
keywords: Acute lung injury; apoptosis; inflammation; polydatin