title: Liquid chromatography-mass spectrometry of biofluids and extracts.
abstract: Metabonomics aims at a comprehensive and semiquantitative monitoring of the perturbations of the metabonome in response to pathophysiological stimuli. Liquid chromatography-mass spectrometry (LC-MS) is one of the most commonly employed analytical platforms for this purpose. As the sample pretreatment is detailed in the former chapter, we here describe the practical procedures for the LC-MS-based metabolic profiling of biological samples including detailed liquid chromatographic and MS conditions, batch analysis, peak alignment, data quality assessment, and compound identification.
authors: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, 16023, Dalian, China.
authors: Xinjie
authors: X
authors: Zhao
authors: Lina
authors: L
authors: Zhou
authors: Peiyuan
authors: P
authors: Yin
authors: Guowang
authors: G
authors: Xu
mesh_terms: D001826:Body Fluids / Q000378:metabolism*; D002457:Cell Extracts / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D025341:Principal Component Analysis; D011786:Quality Control; D014020:Tissue Extracts / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D014020:Tissue Extracts