title: The expression of propionicin PLG-1 gene (plg-1) by lactic starters.
abstract: Propionicin PLG-1 is a bacteriocin produced by Propionibacterium thoenii P127. Such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Yersinia enterocolitica and a strain of Corynebacterium sp. In the present study, plg-1 gene expressing propionicin PLG-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. The PCR product containing plg-1 gene was transferred to different lactic acid bacterial (LAB) strains using pLEB590 as a cloning vector to give the modified vector pLEBPLG-1. LAB transformants showed an antimicrobial activity against Esch. coli DH5α (most affected strain), Listeria monocytogenes 18116, and Salmonella enterica 25566 as model pathogenic strains. Such LAB transformants can be used in dairy industry to control the food-borne pathogens that are largely distributed worldwide and to feed schoolchildren in the poor countries where dangerous epidemic diseases and diarrhoea prevail.
authors: Department of Food Technology,Arid Lands Cultivation Research Institute,City for Scientific Research and Technological Applications,Borg El-Arab,Egypt.
authors: Sameh E
authors: SE
authors: Mohamed
authors: Department of Dairy Science and Technology, Faculty of Agriculture,Alexandria University,Alexandria,Egypt.
authors: Mahmoud K
authors: MK
authors: Tahoun
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D003611:Dairy Products / Q000382:microbiology; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; C079796:propionicin PLG-1
keywords: food-borne pathogens