title: Investigation of the interactions of silibinin with 2-hydroxypropyl-β-cyclodextrin through biophysical techniques and computational methods.
abstract: Cyclodextrins (CDs) are a well-known class of supermolecules that have been widely used to protect drugs against conjugation and metabolic inactivation as well as to enhance the aqueous solubility and hence to ameliorate the oral bioavailability of sparingly soluble drug molecules. The hepatoprotectant drug silibinin can be incorporated into CDs, and here we elucidate the interaction between the drug and the host at the molecular level. The complexation product of silibinin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) is characterized by Differential Scanning Calorimetry, mass spectrometry, solid and liquid high-resolution NMR spectroscopy. The chemical shift changes using (13)C CP/MAS on the complexing of the guest with the host provided significant information on the molecular interactions, and they were in agreement with the 2D NOESY results. These results point out that in both solid and liquid forms, the drug is engulfed and interacts with HP-β-CD in identical manner. Molecular dynamics calculations have been performed to examine the thermodynamic characteristics associated with the silibinin-HP-β-CD interactions and to study the stability of the complex. To approximate the physiological conditions, the aqueous solubility and dissolution characteristics of the complex at pH states simulating those of the upper gastrointestinal tract have been applied. To evaluate the antiproliferative activity of silibinin-HP-β-CD complex comparatively to silibinin in MCF-7 human cancer cells, MTT assays have been performed.
authors: Department of Chemistry, National and Kapodistrian University of Athens , Panepistimiopolis Zografou 15771, Greece.
authors: Tahsin F
authors: TF
authors: Kellici
authors: Dimitrios
authors: D
authors: Ntountaniotis
authors: Georgios
authors: G
authors: Leonis
authors: Maria
authors: M
authors: Chatziathanasiadou
authors: Alexandra V
authors: AV
authors: Chatzikonstantinou
authors: Johanna
authors: J
authors: Becker-Baldus
authors: Clemens
authors: C
authors: Glaubitz
authors: Andreas G
authors: AG
authors: Tzakos
authors: Kyriakos
authors: K
authors: Viras
authors: Petros
authors: P
authors: Chatzigeorgiou
authors: Stavros
authors: S
authors: Tzimas
authors: Evangelia
authors: E
authors: Kefala
authors: Georgia
authors: G
authors: Valsami
authors: Helen
authors: H
authors: Archontaki
authors: Manthos G
authors: MG
authors: Papadopoulos
authors: Thomas
authors: T
authors: Mavromoustakos
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001702:Biopharmaceutics; D055592:Biophysical Phenomena; D049109:Cell Proliferation / Q000187:drug effects; D004347:Drug Interactions; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage* / Q000737:chemistry*; D012995:Solubility; D047392:beta-Cyclodextrins / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D020011:Protective Agents; D012838:Silymarin; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000077385:Silybin
keywords: 2-hydroxypropyl-β-cyclodextrin; MTT assay; NMR spectroscopy; differential scanning calorimetry; molecular dynamics; silibinin or silybin