title: Potential use of curcumin loaded carboxymethylated guar gum grafted gelatin film for biomedical applications.
abstract: Present study describes the synthesis of carboxylmethyl guar gum (CMGG) from the native guar gum (GG). Further, the prepared CMGG is grafted with gelatin to form CMGG-g-gelatin and then mixed with curcumin to prepare a biomaterial. The resultant biomaterial is subjected to the analysis of (1)H NMR, ATR-FTIR, TGA, SEM and XRD ensure the carboxymethylation and grafting. The results reveal that 45% of the amine groups of gelatin have been reacted with the--COOH group of CMGG and 90-95% of curcumin is released from CMGG-g-gelatin after 96h of incubation in the phosphate buffer at physiological pH. In vitro cell line studies reveal the biocompatibility of the biomaterial and the antimicrobial studies display the growth inhibition against gram +ve and gram -ve organisms at a considerable level. Overall, the study indicates that the incorporation of curcumin into CMGG-g-gelatin can improve the functional property of guar gum as well as gelatin.
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C road, Kolkata 700009, West Bengal India.
authors: Piyali Jana
authors: PJ
authors: Manna
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C road, Kolkata 700009, West Bengal India. Electronic address: tapasbiochem1@gmail.com.
authors: Tapas
authors: T
authors: Mitra
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C road, Kolkata 700009, West Bengal India.
authors: Nilkamal
authors: N
authors: Pramanik
authors: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu India.
authors: V
authors: V
authors: Kavitha
authors: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu India.
authors: A
authors: A
authors: Gnanamani
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C road, Kolkata 700009, West Bengal India. Electronic address: ppk923@yahoo.com.
authors: P P
authors: PP
authors: Kundu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D020811:Biomedical Technology; D048430:Cell Shape / Q000187:drug effects; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D005685:Galactans / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008351:Mannans / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D053149:Plant Gums / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C015233:gelatin film; D005780:Gelatin; C007894:guar gum; D003474:Curcumin
keywords: Curcumin; Gelatin; Guar gum