title: Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta.
abstract: In this study, a previously unknown fungal immunomodulatory protein (FIP), here called FIP-ppl, was identified from the basidiomycete fungus Postia placenta by searching its genome sequence database using known FIPs as baits, which was the first basidiomycete FIP to be identified outside the order of edible macro fungi. The gene FIP-ppl was synthesized and expressed in Escherichia coli to produce a glutathione S-transferase (GST) fusion protein. The fusion protein was purified on a GST affinity column and the protein tag was removed using in situ thrombin cleavage. The purified recombinant protein (rFIP-ppl) displayed hemagglutination activity toward rabbit red blood cells but not against human red blood cells. RFIP-ppl stimulated mouse splenocyte cell proliferation and enhanced interleukin-2 (IL-2) release. Antitumor assays indicated that rFIP-ppl had significant cell proliferation inhibitory activity and apoptotic effects in human tumor cells with more pronounced inhibiting proliferation and inducing apoptotic effects on gastric tumor cells (MGC823) than against hepatoma (HepG2) cells. This study confirms an alternative means of identifying, producing, and isolating new FIPs. It may provide convenient access to FIP-ppl with potential human therapeutic applications.
authors: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors: Shu Ying
authors: SY
authors: Li
authors: Institute of Animal Science and Veterinary Medicine, CAAS, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors: Li Jun
authors: LJ
authors: Shi
authors: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors: Yang
authors: Y
authors: Ding
authors: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors: Ying
authors: Y
authors: Nie
authors: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. Electronic address: tangxuanming@caas.cn.
authors: Xuan Ming
authors: XM
authors: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D055453:Coriolaceae / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D006384:Hemagglutination; D056945:Hep G2 Cells; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase
keywords: Aggregation; Antitumor; Fungal immunomodulatory protein (FIP); Mitogen; Postia placenta