title: Fusion coat protein of pumpkin yellow vein mosaic virus with maltose binding protein: Applications in immunodiagnosis of begomoviruses.
abstract: Availability of adequate quantity of purified virus preparation from plant tissue is the major limitation in producing polyclonal antibodies (PAb) to begomovirus. Very few examples show successful utilization of E. coli expressed recombinant coat protein (CP) for immuno diagnosis of begomoviruses. In the present study, ~771 bp CP gene (~29.0 kDa) of Pumpkin yellow vein mosaic virus (PYVMV) was expressed as a ~71.0 kDa fusion protein with maltose binding protein (MBP) (~42.0 kDa) in E. coli. The MBP-CP was obtained in soluble state. The PAb to the purified fusion protein successfully detected PYVMV and other bipartite and monopartite begomoviruses in the field samples at 1:250 dilution in enzyme linked immunosorbent assay. Our study for the first time showed that MBP-tag fusion CP was suitable to produce diagnostic antibody to begomoviruses.
authors: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India ; Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, 522 510 Andhra Pradesh India.
authors: Chigurupati
authors: C
authors: Phaneendra
authors: Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, 522 510 Andhra Pradesh India.
authors: K R S
authors: KR
authors: Sambasiva Rao
authors: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors: Reetika
authors: R
authors: Kapoor
authors: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors: R K
authors: RK
authors: Jain
authors: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors: Bikash
authors: B
authors: Mandal
keywords: Coat protein gene; ELISA; Pumpkin yellow vein mosaic virus; Recombinant protein; Western blot