title: Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus).
abstract: Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow.
authors: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors: Jundan
authors: J
authors: Duan
authors: Gongqing
authors: G
authors: Feng
authors: Pei
authors: P
authors: Chang
authors: Xueyan
authors: X
authors: Zhang
authors: Qingchun
authors: Q
authors: Zhou
authors: Xueping
authors: X
authors: Zhong
authors: Chao
authors: C
authors: Qi
authors: Songguang
authors: S
authors: Xie
authors: Haobin
authors: H
authors: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D003530:Cyprinidae / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D017403:In Situ Hybridization; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C539707:dnd1 protein, zebrafish