title: [Efficacy of PCR for the differential diagnosis of tuberculosis in granulomatous lesions of paraffin-embedded formalin fixed tissues].
abstract: BACKGROUND
  Granulomatous lesions occur in tuberculosis (TB), other infections, toxic, allergic, and autoimmune diseases among others. In absence of a an acid-fast bacilli (AFB) confirmation of TB is necessary.
  
  
  OBJECTIVE
  To assess the efficacy of PCR for TB detection and to correlate with granuloma histology and AFB staining.
  
  
  METHODS
  We analyzed 380 fixed paraffin-embedded tissues (PETs) of granulomas with and without caseous necrosis; suppurative; sarcoidal; or of chronic nonspecific nature. Nested PCR-IS6110 for Mycobacterium tuberculosis complex (MTB) and a nested pan-Mycobacterium for the hsp65 gene were used for Mycobacterium spp detection.
  
  
  RESULTS
  PCR was more sensitive than AFB staining for all five catagories of granulomas: G1: PCR 71%, AFB staining 28%. G2: PCR 37%, AFB 8%. G3: PCR 17%, AFB staining 7%. G4: PCR 8%, AFB staining 4%. G5: PCR 6%, AFB staining 0%.
  
  
  CONCLUSIONS
  Molecular diagnosis of TB using PCR-based testing is a fast, efficacious and sensitive method that increased the accuracy of PET histological diagnosis associated with granulomatous lesions.
authors: Sonia
authors: S
authors: Montenegro
authors: Carolina
authors: C
authors: Delgado
authors: Susana
authors: S
authors: Pineda
authors: Cristian
authors: C
authors: Reyes
authors: Tiare de la
authors: Tde L
authors: Barra
authors: Claudia
authors: C
authors: Cabezas
authors: Loreto
authors: L
authors: Spencer
authors: Francisco
authors: F
authors: Mucientes
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D005557:Formaldehyde; D006099:Granuloma / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D016707:Tissue Fixation; D014376:Tuberculosis / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D005557:Formaldehyde