title: [Expression of autophagy related gene BECLIN-1 and number of autophagic vacuoles in bone marrow mononuclear cells from 40 myelodysplastic syndromes patients and their significance].
abstract: OBJECTIVE
  The purpose of this study was to detect the expression level of autophagy related gene BECLIN-1 and the number of autophagic vacuoles in bone marrow mononuclear cells (BMMNC) from myelodysplastic syndrome(MDS) patients and to explore their difference in different stage of MDS and relationship between their difference and disease characteristics.
  
  
  METHODS
  The BMMNC from 9 normal controls, 19 cases of low-risk MDS, 14 cases of high-risk MDS and 7 cases of MDS-transformed AML were collected. The expression level of BECLIN-1 was detected by real time PCR (RT-PCR) and the amount of autophagic vacuoles was counted by transmission electron microscopy.
  
  
  RESULTS
  The expression level of BECLIN-1 in BMMNC from patients with low-risk group was obviously higher than that in BMMNC from normal controls; the expression level of BECLIN-1 in BMMNC from patients of hgh risk group was higher than that in BMMNC of normal group, but there was no statistical significance (P > 0.05); the expression level of BECLIN-1 in BMMNC from patients with MDS-transformed AML group was significanly lower than that in BMMNC of normal group (P < 0.05). Transinission electron microscopy showed that the amount of autophagic vacuoles in BMMNC from patients with low-risk and high-risk MDS groups was more than that in normal control, but there was no stetistcal significance (P > 0.05), while the amount of autopuagic vecuoles in BMMNC from patients of MDS-transformed AML group was significantly less (P < 0.05).
  
  
  CONCLUSIONS
  The expression of BECLIN-1 and the number of autophagic vacuoles in BMMNC from patients with MDS progression and patients with MDS-transformed AML are gradually declining. The autophagy may be associated with disease progression.
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Bin
authors: B
authors: Hu
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Qin-Fang
authors: QF
authors: Yue
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Ye
authors: Y
authors: Chen
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Fan-Dan
authors: FD
authors: Bu
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Chun-Yan
authors: CY
authors: Sun
authors: Institute of Hematology, Union Hospital, Meclical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. E-mail: lxy87306548@sina.com.
authors: Xin-Yue
authors: XY
authors: Liu
mesh_terms: D051017:Apoptosis Regulatory Proteins; D001343:Autophagy; D000071186:Beclin-1; D001853:Bone Marrow; D001854:Bone Marrow Cells; D018450:Disease Progression; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D008565:Membrane Proteins; D009190:Myelodysplastic Syndromes; D014617:Vacuoles
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins