title: Effect of aerobic exercise training on non-alcoholic fatty liver disease induced by a high fat diet in C57BL/6 mice.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of aerobic exercise training on a high fat diet (HFD)-induced fatty liver and its metabolic complications in C57BL/6 mice.
  
  
  METHODS
  Mice at 5-month old (n = 30) were randomly assigned to standard chow (SC + CON, n = 10) and high-fat diet (HFD, n = 20), and they were subjected to SC and HFD, respectively, for 23-week. After 15-week of HFD, mice in the HFD group were further assigned to HFD (HFD + CON, n = 10) or exercise training (HFD + EX, n = 10) groups. The HFD + EX mice were subjected to aerobic treadmill running during the last 8-week of the 23-week HFD course. Outcomes included hepatic steatosis, insulin resistance, and expression of genes involved in mitochondrial function and/or fatty oxidation as well as de novo lipogenesis and/or triacylglycerol (TAG) synthesis.
  
  
  RESULTS
  Treadmill running ameliorated impaired glucose tolerance and insulin resistance secondary to the HFD. The beneficial effects of treadmill running were associated with enhanced molecular markers of mitochondrial function and/or fatty acids oxidation (i.e., PPARα and CPT1a mRNAs, pAMPK/AMPK, pACC, and SIRT1 protein) as well as suppressed expression of de novo lipogenesis and/or TAG synthesis (i.e., SREBP1c, lipin1 and FAS mRNAs) in the liver.
  
  
  CONCLUSIONS
  The current findings suggest that aerobic exercise training is an effective and non-pharmacological means to combat fatty liver and its metabolic complications in HFD-induced obese mice.
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Jinkyung
authors: J
authors: Cho
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Inhwan
authors: I
authors: Lee
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Donghyun
authors: D
authors: Kim
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Yeojung
authors: Y
authors: Koh
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Jiyoung
authors: J
authors: Kong
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Sanghee
authors: S
authors: Lee
authors: College of Sport Science, Sungkyunkwan University, Suwon, Korea.
authors: Hyunsik
authors: H
authors: Kang
keywords: AMPK; aerobic exercise; high-fat diet; insulin resistance; non-alcoholic fatty liver