title: Simple and efficient methodology to determine mycotoxins in cereal syrups.
abstract: Consumption of cereal syrups is increasing nowadays. Mycotoxins may be found in syrups resulting from the use of contaminated raw material or invading microorganisms in the final manufactured product. However, these matrices have been scarcely explored regarding their mycotoxin content. A sensitive, simple and rapid method for the determination of ten mycotoxins (ochratoxin A, fumonisin B1, fumonisin B2, deoxynivalenol, fusarenon-X, T-2 and HT-2 toxin, citrinin, sterigmatocystin and zearalenone) in cereal syrups (rice, wheat and barley) has been developed and characterised using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and a sample treatment based on QuEChERS procedure. Matrix-matched calibration curves were established and limits of quantification were below the limits usually established by current legislation in different foodstuff. The relative standard deviation of the whole analytical method was lower than 12% in all cases, while recoveries ranged from 70.2% to 100.6%, therefore fulfilling the current requirements for mycotoxins analysis.
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Natalia
authors: N
authors: Arroyo-Manzanares
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: José F
authors: JF
authors: Huertas-Pérez
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Laura
authors: L
authors: Gámiz-Gracia
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain. Electronic address: amgarcia@ugr.es.
authors: Ana M
authors: AM
authors: García-Campaña
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis; D009183:Mycotoxins / Q000032:analysis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009183:Mycotoxins
keywords: Cereal syrups; Multiclass mycotoxins; QuEChERS; UHPLC–MS/MS