title: Plasmid DNA-coding p62 as a bone effective anti-inflammatory/anabolic agent.
abstract: We recently reported that a DNA plasmid coding p62-SQSTM1 acts as an effective anti tumor vaccine against both transplantable mouse tumors and canine spontaneous mammary neoplasms. Here we report the unexpected finding that intramuscular delivery of p62 DNA exerts a powerful anti-osteoporotic activity in a mouse model of inflammatory bone loss (i.e, ovariectomy) by combining bone-sparing and osteo-synthetic effects. Notably, the suppression of osteoporosis by p62DNA was associated with a sharp down-regulation of master inflammatory cytokines, and up-regulation of endogenous p62 protein by bone-marrow stromal cells. The present data provide a solid rational to apply p62 DNA vaccine as a safe, new therapeutic for treatment of inflammatory related bone loss diseases.
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Maria Giovanna
authors: MG
authors: Sabbieti
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Dimitrios
authors: D
authors: Agas
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Melania
authors: M
authors: Capitani
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Luigi
authors: L
authors: Marchetti
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Antonio
authors: A
authors: Concetti
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Cecilia
authors: C
authors: Vullo
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Giuseppe
authors: G
authors: Catone
authors: CureLab Oncology Inc, Needham MA (USA).
authors: Vladimir
authors: V
authors: Gabai
authors: CureLab Oncology Inc, Needham MA (USA).
authors: Victor
authors: V
authors: Shifrin
authors: Dept. Biochem, Boston University School of Medicine, Boston MA (USA).
authors: Michael Y
authors: MY
authors: Sherman
authors: CureLab Oncology Inc, Needham MA (USA).
authors: Alexander
authors: A
authors: Shneider
authors: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (Italy).
authors: Franco M
authors: FM
authors: Venanzi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000494:pharmacology*; D011897:Random Allocation; D000071456:Sequestosome-1 Protein; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000893:Anti-Inflammatory Agents; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; D019444:Vaccines, DNA; D004247:DNA