title: HPLC/MS analysis of polyphenols, antioxidant and antimicrobial activities of Artabotrys hildebrandtii O. Hffm. extracts.
abstract: The purpose of this work was to evaluate chemical constituents, antioxidant and antimicrobial activities of Artabotrys hildebrandtii, an endemic medicinal plant from Madagascar. Ethanol extracts from the leaves and stem bark were tested to evaluate DPPH free radical scavenging, using butylated hydroxytoluene and quercetin as standard antioxidants. An high-performance liquid chromatography/mass spectrometry method was developed to investigate the presence of phenolic compounds in the studied samples; gentisic acid, chlorogenic acid, hyperoside, isoquercitrin, rutin, quercitrin, quercetol, apigenin and luteolin were identified. Total polyphenolic content was determined by a spectrophotometric method using Folin-Ciocâlteu reagent. Results showed the efficiency of A. hildebrandtii leaves extract against strains of Staphylococcus aureus and Listeria monocytogenes, as the inhibitory activity is more powerful compared to Gentamicin, used as the standard drug. The leaves of A. hildebrandtii can be considered an important source of polyphenols, especially of rutin, with good antioxidant and antimicrobial activities.
authors: a Department of Chemistry, Faculty of Science , University of Antsiranana , Antsiranana 201 , Madagascar.
authors: Julio Hervé
authors: JH
authors: Andriamadio
authors: b Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique, Department of Organic Chemistry , University of Antananarivo , P.O. Box 906, Antananarivo 101 , Madagascar.
authors: Léa Herilala
authors: LH
authors: Rasoanaivo
authors: c Department of Pharmacognosy , University of Medicine and Pharmacy 'Iuliu Hatieganu' , 12 I. Creangă Street, Cluj-Napoca 400010 , Romania.
authors: Daniela
authors: D
authors: Benedec
authors: d Department of Pharmaceutical Technology and Biopharmaceutics , University of Medicine and Pharmacy 'Iuliu Hatieganu' , 12 I. Creangă Street, Cluj-Napoca 400010 , Romania.
authors: Laurian
authors: L
authors: Vlase
authors: d Department of Pharmaceutical Technology and Biopharmaceutics , University of Medicine and Pharmacy 'Iuliu Hatieganu' , 12 I. Creangă Street, Cluj-Napoca 400010 , Romania.
authors: Ana-Maria
authors: AM
authors: Gheldiu
authors: e State Veterinary Laboratory for Animal Health and Safety , 1 Piata Marasti Street, Cluj-Napoca 400609 , Romania.
authors: Mihaela
authors: M
authors: Duma
authors: c Department of Pharmacognosy , University of Medicine and Pharmacy 'Iuliu Hatieganu' , 12 I. Creangă Street, Cluj-Napoca 400010 , Romania.
authors: Anca
authors: A
authors: Toiu
authors: b Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique, Department of Organic Chemistry , University of Antananarivo , P.O. Box 906, Antananarivo 101 , Madagascar.
authors: Amélie
authors: A
authors: Raharisololalao
authors: c Department of Pharmacognosy , University of Medicine and Pharmacy 'Iuliu Hatieganu' , 12 I. Creangă Street, Cluj-Napoca 400010 , Romania.
authors: Ilioara
authors: I
authors: Oniga
mesh_terms: D029061:Annonaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D052978:Disk Diffusion Antimicrobial Tests; D008089:Listeria monocytogenes / Q000187:drug effects; D008270:Madagascar; D013058:Mass Spectrometry; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
keywords: Artabotrys hildebrandtii; Madagascar; antimicrobial; antioxidant; polyphenols