title: Characterization of particle morphology of biochanin A molecularly imprinted polymers and their properties as a potential sorbent for solid-phase extraction.
abstract: Molecularly imprinted polymers (MIPs) with biochanin A as a template were obtained using a bulk polymerization with non-covalent imprinting approach. The polymers were prepared in acetonitrile as porogen, using ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The synthesis, with an application of 1',1'-azobis(cyclohexanecarbonitrile) (ACHN) as an initiator, has been performed thermally. During the synthesis process the effect of different functional monomers such as methacrylic acid (MAA), acrylamide (AA) and 4-vinylpyridine (4-VP) was investigated. The application of nitrogen sorption porosimetry, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) permitted the characterization and evaluation of synthesized polymers. The adsorption capacity of obtained MIPs was checked by using the binding testing. All synthesized polymers were evaluated as solid-phase extraction (SPE) sorbents for isolation and preconcentration of biochanin A and its analogues, daidzein and genistein. The MIPs exhibited higher affinity for biochanin A over competitive compounds.
authors: Opole University, Faculty of Chemistry, Pl. Kopernika 11a, 45-040 Opole, Poland.
authors: Anna M
authors: AM
authors: Chrzanowska
authors: Opole University, Faculty of Chemistry, Pl. Kopernika 11a, 45-040 Opole, Poland. Electronic address: Anna.Poliwoda@uni.opole.pl.
authors: Anna
authors: A
authors: Poliwoda
authors: Opole University, Faculty of Chemistry, Pl. Kopernika 11a, 45-040 Opole, Poland.
authors: Piotr P
authors: PP
authors: Wieczorek
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D020106:Acrylamide / Q000737:chemistry; D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D019833:Genistein / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D054802:Molecular Imprinting / Q000379:methods; D009584:Nitrogen / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000097:Acetonitriles; D003432:Cross-Linking Reagents; D007529:Isoflavones; D008689:Methacrylates; D011108:Polymers; D011725:Pyridines; C008384:methacrylic acid; D020106:Acrylamide; C004742:daidzein; C004919:ethylene dimethacrylate; D019833:Genistein; C029351:4-vinylpyridine; D009584:Nitrogen; C004541:biochanin A; C032159:acetonitrile
keywords: Adsorption; Molecular imprinting; Phytoestrogens; Solid-phase extraction