title: Potential use of gallium-doped phosphate-based glass material for periodontitis treatment.
abstract: This study aimed at evaluating the potential effect of gallium-incorporated phosphate-based glasses towards periodontitis-associated bacteria, Porphyromonas gingivalis, and matrix metalloproteinase-13. Periodontitis describes a group of inflammatory diseases of the gingiva and supporting structures of the periodontium. They are initiated by the accumulation of plaque bacteria, such as the putative periodontal pathogen Porphyromonas gingivalis, but the host immune response such as elevated matrix metalloproteinases are the major contributing factor for destruction of periodontal tissues. Antibacterial assays of gallium-incorporated phosphate-based glasses were conducted on Porphyromonas gingivalis ATCC 33277 using disc diffusion assay on fastidious anaerobe agar and liquid broth assay in a modified tryptic soy broth. In vitro study investigated the effect of gallium on purified recombinant human matrix metalloproteinase-13 activity using matrix metalloproteinase assay kit. In vivo biocompatibility of gallium-incorporated phosphate-based glass was evaluated in rats as subcutaneous implants. Antibacterial assay of gallium displayed activity against Porphyromonas gingivalis (inhibition zone of 22 ± 0.5 mm compared with 0 mm for control glass, c-PBG). Gallium in the glass contributed to growth inhibitory effect on Porphyromonas gingivalis (up to 1.30 reductions in log 10 values of the viable counts compared with control) in a modified tryptic soy broth. In vitro study showed gallium-incorporated phosphate-based glasses inhibited matrix metalloproteinase activity significantly (p ≤ 0.01) compared with c-PBG. Evaluation of in vivo biocompatibility of gallium-incorporated phosphate-based glasses in rats showed a non-toxic and foreign body response after 2 weeks of implantation. The results indicate that gallium ions might act on multiple targets of biological mechanisms underlying periodontal disease. Moreover, gallium-incorporated phosphate-based glasses are biocompatible in a rat model. The findings warrant further investigation and will have important clinical implications in the future treatment and management of periodontitis.
authors: Department of Health Services Research and School of Dentistry, University of Liverpool, Liverpool, UK.
authors: Rohan
authors: R
authors: Sahdev
authors: Department of Surgical Research, Imperial College London, Watford Road, Harrow, UK.
authors: Tahera I
authors: TI
authors: Ansari
authors: Department of Health Services Research and School of Dentistry, University of Liverpool, Liverpool, UK.
authors: Susan M
authors: SM
authors: Higham
authors: Department of Health Services Research and School of Dentistry, University of Liverpool, Liverpool, UK S.Valappil@iverpool.ac.uk.
authors: Sabeel P
authors: SP
authors: Valappil
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D016866:Bacteroidaceae Infections / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D001672:Biocompatible Materials / Q000737:chemistry*; D005708:Gallium / Q000008:administration & dosage / Q000494:pharmacology*; D005898:Glass / Q000737:chemistry; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000276:immunology; D061965:Matrix Metalloproteinase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D010518:Periodontitis / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D010710:Phosphates / Q000737:chemistry*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D061965:Matrix Metalloproteinase Inhibitors; D010710:Phosphates; D005708:Gallium; D053509:Matrix Metalloproteinase 13
keywords: Gallium; Porphyromonas gingivalis; matrix metalloproteinase; periodontitis; phosphate-based glasses