title: Cloning and expression of visfatin and screening of oligopeptides binding with visfatin.
abstract: OBJECTIVE
  In the present study, we constructed an efficient expression system for visfatin and identified the peptide sequences which binding to visfatin.
  
  
  RESULTS
  The fusion protein was identified by SDS-PAGE and Western blot. The yield of visfatin was 300 mg/L culture medium with optimal conditions. The recombinant visfatin binds with insulin receptor with a dose-dependent effect. All of the 24 sequence identified by ELISA were able to bind to visfatin specifically. Among the 24 DNA sequences, there were 8 clones of AAKTPTE, 4 clones of ATTVPAS and 4 clones of MSLQQEH.
  
  
  CONCLUSIONS
  The sequence of AA(X)TPT(X) was the most frequently existed sequence in all of sequences analyzed, which suggests that AA(X)TPT(X) is likely to be the essential motif in peptides which visfatin could bind with.
authors: Ministry of Health Key Laboratory of Hormone and Development, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China ; Department of Diabetic Gastroenterology, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China.
authors: Xin Yu
authors: XY
authors: Liu
authors: Department of Biochemistry and Molecular Biology, Basic Medical College, Tianjin Medical University Tianjin 300070, P. R. China ; Tianjin Key Laboratory of Cellular and Molecular Immunology Tianjin 300070, P. R. China ; Key Laboratory of Educational Ministry of China Tianjin 300070, P. R. China.
authors: Lin
authors: L
authors: Ge
authors: Ministry of Health Key Laboratory of Hormone and Development, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China ; Department of Diabetic Gastroenterology, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China.
authors: De Min
authors: DM
authors: Yu
keywords: Visfatin; adipokine; fusion expression; gene engineering; phage display