title: Evaluation of phenotypic screening tests for carbapenemase production in Pseudomonas aeruginosa from patients with cystic fibrosis.
abstract: Carbapenemase phenotypic tests (inhibitor-based, modified Hodge and Carba NP) for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF) were evaluated with PCR as a reference assay. Except for Carba NP, all methods demonstrated high false positives. Carba NP test presents a promising screening method for CF P. aeruginosa isolates.
authors: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia; Adult Cystic Fibrosis Centre, Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia; School of Medicine, The University of Queensland, Brisbane, Australia. Electronic address: anna.tai@uq.net.au.
authors: Anna S
authors: AS
authors: Tai
authors: University of Queensland, UQ Centre for Clinical Research (UQCCR), Brisbane, Australia. Electronic address: h.sidjabat@uq.edu.au.
authors: Hanna E
authors: HE
authors: Sidjabat
authors: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: t.m.kidd@uq.edu.au.
authors: Timothy J
authors: TJ
authors: Kidd
authors: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: d.whiley@uq.edu.au.
authors: David M
authors: DM
authors: Whiley
authors: University of Queensland, UQ Centre for Clinical Research (UQCCR), Brisbane, Australia. Electronic address: d.paterson1@uq.edu.au.
authors: David L
authors: DL
authors: Paterson
authors: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia; Adult Cystic Fibrosis Centre, Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia; QIMR Berghofer Medical Research Institute, Brisbane, Australia. Electronic address: Scott.Bell@health.qld.gov.au.
authors: Scott C
authors: SC
authors: Bell
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001431:Bacteriological Techniques; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D005189:False Positive Reactions; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000150:complications / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carba NP test; Cystic fibrosis; Metallo-β-lactamase; Modified Hodge test; PCR; Pseudomonas aeruginosa