title: Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures.
abstract: The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(-1) dry weight (about 60 mg L(-1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(-1) dry weight (200-210 mg L(-1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis.
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Marco
authors: M
authors: Taurino
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Ilaria
authors: I
authors: Ingrosso
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Leone
authors: L
authors: D'amico
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Stefania
authors: S
authors: De Domenico
authors: Institute of Chemical Methodologies, CNR, Area della Ricerca di Roma 1, via Salaria km 29,300, Monterotondo Stazione, Rome, Italy.
authors: Isabella
authors: I
authors: Nicoletti
authors: Institute of Chemical Methodologies, CNR, Area della Ricerca di Roma 1, via Salaria km 29,300, Monterotondo Stazione, Rome, Italy.
authors: Danilo
authors: D
authors: Corradini
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Angelo
authors: A
authors: Santino
authors: Institute of Food Production Sciences, C.N.R. Unit of Lecce, via Monteroni, 73100 Lecce, Italy.
authors: Giovanna
authors: G
authors: Giovinazzo
keywords: 12-oxo-phytodienoic; Chitosan; Coronatine; Jasmonic acid; Methyl jasmonate; Viniferins; Vitis vinifera cv. Negramaro