title: Optimization of volatile fatty acids and hydrogen production from Saccharina japonica: acidogenesis and molecular analysis of the resulting microbial communities.
abstract: Response surface methodology (RSM) was used to optimize the production of volatile fatty acids (VFAs) and hydrogen from mixed anaerobic cultures of Saccharina japonica with respect to two independent variables: methanogenic inhibitor concentration and temperature. The effects of four methanogenic inhibitors on acidogenic processes were tested, and qualitative microbial analyses were carried out. Escherichia, Acinetobacter, and Clostridium were the most predominant genera in samples treated with chloroform (CHCl3), iodoform (CHI3), 2-bromoethanesulfonate (BES), or β-cyclodextrin (β-CD), respectively. RSM showed that the production of VFAs reached a peak of 12.5 g/L at 38.6 °C in the presence of 7.4 g/L β-CD; these were the conditions under which hydrogen production was also nearly maximal. The quantitative polymerase chain reaction (qPCR) showed that shifts in the bacterial community population correlated with the concentrations of β-CD indicating that this compound effectively inhibited methanogens.
authors: Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
authors: Kwonsu
authors: K
authors: Jung
authors: Woong
authors: W
authors: Kim
authors: Gwon Woo
authors: GW
authors: Park
authors: Charles
authors: C
authors: Seo
authors: Ho Nam
authors: HN
authors: Chang
authors: Yeu-Chun
authors: YC
authors: Kim
mesh_terms: D017738:Alkanesulfonic Acids / Q000494:pharmacology; D000693:Anaerobiosis; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002725:Chloroform / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000096:biosynthesis*; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism*; D008697:Methane / Q000378:metabolism; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics; D000459:Phaeophyceae / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S; D013696:Temperature; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D017738:Alkanesulfonic Acids; D005232:Fatty Acids, Volatile; D006847:Hydrocarbons, Iodinated; D012336:RNA, Ribosomal, 16S; D047392:beta-Cyclodextrins; C018853:2-bromoethanesulfonic acid; D002725:Chloroform; D006859:Hydrogen; C031215:betadex; C010473:iodoform; D008697:Methane