title: Relationships between the genome and some phenotypical properties of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk.
abstract: Lactobacillus fermentum CECT 5716, isolated from human milk, has immunomodulatory, anti-inflammatory, and anti-infectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, some phenotypic properties of L. fermentum CECT 5716 were evaluated, and the genetic basis for the obtained results was searched for in the strain genome. L. fermentum CECT 5716 does not contain plasmids and showed neither bacteriocin nor biogenic amine biosynthesis ability but was able to produce organic acids, glutathione, riboflavin, and folates and to moderately stimulate the maturation of mouse dendritic cells. No prophages could be induced, and the strain was sensitive to all antibiotics proposed by European Food Safety Authority (EFSA) standards, while no transmissible genes potentially involved in antibiotic resistance were detected in its genome. Globally, there was an agreement between the phenotype properties of L. fermentum CECT 5716 and the genetic information contained in its genome.
authors: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Ciudad Universitaria, Avda. Puerta de Hierro, s/n., 28040, Madrid, Spain.
authors: Nivia
authors: N
authors: Cárdenas
authors: Jonathan E
authors: JE
authors: Laiño
authors: Susana
authors: S
authors: Delgado
authors: Esther
authors: E
authors: Jiménez
authors: Marianela
authors: M
authors: Juárez del Valle
authors: Graciela
authors: G
authors: Savoy de Giori
authors: Fernando
authors: F
authors: Sesma
authors: Baltasar
authors: B
authors: Mayo
authors: Leónides
authors: L
authors: Fernández
authors: Jean Guy
authors: JG
authors: LeBlanc
authors: Juan M
authors: JM
authors: Rodríguez
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005260:Female; D005492:Folic Acid / Q000378:metabolism; D016680:Genome, Bacterial; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008895:Milk, Human / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D019936:Probiotics / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D012256:Riboflavin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D005492:Folic Acid; D005978:Glutathione; D012256:Riboflavin