title: Aniline exposure associated with up-regulated transcriptional responses of three glutathione S-transferase Delta genes in Drosophila melanogaster.
abstract: Complex transcriptional profile of glutathione S-transferase Delta cluster genes occurred in the developmental process of the fruit fly Drosophila melanogaster. The purpose of this project was to quantify the expression levels of Gst Delta class genes altered by aniline exposure and to understand the relationship between aniline dosages and the variation of Gst Delta genes expressed in D. melanogaster. Using RT-PCR expression assays, the expression patterns of the transcript mRNAs of the glutathione S-transferase Delta genes were revealed and their expression levels were measured at eggs, larvae, pupae and adults. The adult stage was selected for further dose-response assays. After analysis, the results indicated that three Gst Delta genes (Gst D2, Gst D5 and Gst D6) were found to show a peak of up-regulated transcriptional response at 6-8h of exposure of aniline. Furthermore, the dose-response relationship of their induction levels within the dose regiments (from 1.2 to 2.0 μl/tube) had been measured. The expression patterns and annotations of these genes were discussed in the context.
authors: Department of Biology, National Changhua University of Education, Changhua 500, Taiwan.
authors: Wen-Chiao
authors: WC
authors: Chan
authors: Department of Biology, National Changhua University of Education, Changhua 500, Taiwan.
authors: Yi-Chih
authors: YC
authors: Chien
authors: Department of Environmental Engineering and Science, Feng Chia University, Taichung 407, Taiwan. Electronic address: cichien@fcu.edu.tw.
authors: Cheng-I
authors: CI
authors: Chien
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D000814:Aniline Compounds; D019476:Insect Proteins; D012333:RNA, Messenger; D005982:Glutathione Transferase; C023650:aniline
keywords: Aniline; Drosophila; Glutathione S-transferases