title: Multiple diguanylate cyclase-coordinated regulation of pyoverdine synthesis in Pseudomonas aeruginosa.
abstract: The nucleotide signalling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) plays an essential role in regulating microbial virulence and biofilm formation. C-di-GMP is synthesized by diguanylate cyclase (DGC) enzymes and degraded by phosphodiesterase (PDE) enzymes. One intrinsic feature of c-di-GMP signalling is the abundance of DGCs and PDEs encoded by many bacterial species. It is unclear whether the different DGCs or PDEs coordinately establish the c-di-GMP regulation or function independently of each other. Here, we provide evidence that multiple DGCs are involved in regulation of c-di-GMP on synthesis of the major iron siderophore pyoverdine in Pseudomonas aeruginosa. Constitutive expression of the WspG or YedQ DGC in P. aeruginosa is able to induce its pyoverdine synthesis. Induction of pyoverdine synthesis by high intracellular c-di-GMP depends on the synthesis of exopolysaccharides and another two DGCs, SiaD and SadC. SiaD was found to boost the c-di-GMP synthesis together with constitutively expressing YedQ. The exopolysaccharides and the SiaD DGC were found to modulate the expression of the RsmY/RsmZ ncRNAs. Induction of the RsmY/RsmZ ncRNAs might enhance the pyoverdine synthesis through SadC. Our study sheds light on a novel multiple DGC-coordinated c-di-GMP regulatory mechanism of bacteria.
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.
authors: Yicai
authors: Y
authors: Chen
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.
authors: Mingjun
authors: M
authors: Yuan
authors: School of Civil and Environmental Engineering, Nanyang Technological University, Nanyang, 639798, Singapore.
authors: Anee
authors: A
authors: Mohanty
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.|Interdisciplinary Graduate School, Nanyang Technological University, Nanyang, 637551, Singapore.
authors: Joey Kuok Hoong
authors: JK
authors: Yam
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.
authors: Yang
authors: Y
authors: Liu
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.|NUS Graduate School of Integrative Sciences and Engineering, National University of Singapore, Nanyang, 117543, Singapore.
authors: Song Lin
authors: SL
authors: Chua
authors: Department of Chemistry, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.
authors: Thomas E
authors: TE
authors: Nielsen
authors: Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, Panum Institute, University of Copenhagen, København N, 2200, Denmark.
authors: Tim
authors: T
authors: Tolker-Nielsen
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.|Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, Panum Institute, University of Copenhagen, København N, 2200, Denmark.
authors: Michael
authors: M
authors: Givskov
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.|School of Civil and Environmental Engineering, Nanyang Technological University, Nanyang, 639798, Singapore.
authors: Bin
authors: B
authors: Cao
authors: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Nanyang, 637551, Singapore.|School of Biological Sciences, Nanyang Technological University, Nanyang, 639798, Singapore.
authors: Liang
authors: L
authors: Yang
mesh_terms: D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009842:Oligopeptides / Q000096:biosynthesis*; D019761:Phosphorus-Oxygen Lyases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D009842:Oligopeptides; C062025:bis(3',5')-cyclic diguanylic acid; C042453:pyoverdin; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP