title: Geno/cytotoxicty and Apoptotic Properties of Phenolic Compounds from the Seeds of Dorema Glabrum Fisch. C.A.
abstract: BACKGROUND
  Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anti-cancer potency of bioactive compounds from the seeds of Dorma glabrum.
  
  
  METHODS
  Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds.
  
  
  RESULTS
  Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 μg/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments.
  
  
  CONCLUSIONS
  This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum.
authors: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Morteza
authors: M
authors: Eskandani
authors: Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Elmira
authors: E
authors: Dadizadeh
authors: Drugs Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Hamed
authors: H
authors: Hamishehkar
authors: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran ; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Hossein
authors: H
authors: Nazemiyeh
authors: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran ; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Jaleh
authors: J
authors: Barar
keywords: Cytotoxicity; Dorema glabrum; Genotoxicity; Phenolic compounds