title: Cultivar specific variations in antioxidative defense system, genome and proteome of two tropical rice cultivars against ambient and elevated ozone.
abstract: For the past few decades continuous increase in the levels of tropospheric ozone (O3) concentrations is posing to be a threat for agricultural productivity. Two high yielding tropical rice cultivars (Malviya dhan 36 and Shivani) were evaluated against different concentrations of O3 under field conditions. Experimental design included filtered chambers, non-filtered chambers having ambient O3 and 10 and 20ppb elevated O3 above the ambient. Study was conducted to assess differential response if any in induction of antioxidative defense system, genome stability, leaf proteome, yield and quality of the product in both the test cultivars. Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR) were induced under ambient and elevated levels of O3. Native polyacrylamide gel electrophoresis (PAGE) of SOD, CAT and POD also displayed increased enzymatic activity along with associated alterations in specific isoforms. Ascorbic acid, thiols and phenolics were also stimulated at ambient and elevated O3. Structural alterations in DNA of rice plants due to O3 affecting its genome template stability (GTS) was examined using RAPD technique. 2-D PAGE revealed 25 differential spots in Malviya dhan 36 and 36 spots in Shivani after O3 treatment with reductions in RuBisCO subunits. Reductions in yield and change in the quality of grains were also noticed.
authors: Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: abhijitbhu@gmail.com.
authors: Abhijit
authors: A
authors: Sarkar
authors: Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: abha2512.singh@gmail.com.
authors: Aditya Abha
authors: AA
authors: Singh
authors: Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: sbagrawal56@gmail.com.
authors: Shashi Bhushan
authors: SB
authors: Agrawal
authors: Department of Botany, Aligarh Muslim University, Aligarh 202001, India. Electronic address: ahmadaltaf@yahoo.com.
authors: Altaf
authors: A
authors: Ahmad
authors: Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: shashi.bhubotany@gmail.com.
authors: Shashi Pandey
authors: SP
authors: Rai
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D018745:Genome, Plant; D005980:Glutathione Reductase / Q000378:metabolism; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010126:Ozone / Q000633:toxicity*; D009195:Peroxidase / Q000378:metabolism; D011110:Polymorphism, Genetic; D020543:Proteome / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020543:Proteome; D010126:Ozone; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Antioxidative enzymes; DNA; Grain quality; Native PAGE; Tropospheric ozone; Yield