title: Development of miniaturized sorbent membrane funnel-based spray platform for biological analysis.
abstract: In this work, a miniaturized solid-phase extraction (SPE) platform, called sorbent membrane funnel, which permits in situ cleanup prior to membrane funnel-based spray analysis was developed. The fabrication of funnel and the mounting of SPE sorbent were simple and straightforward by a homemade punching system. Using different sorbents, the SPE sorbent funnel has been successfully applied in spray analysis of drug molecules spiked in human plasma, trypsin digested solution of bovine serum albumin in the presence of high concentration of chaotropic reagents, and phosphopeptides in the tryptic digested solution of casein. The results demonstrated that SPE sorbent attached membrane funnels can be a useful tool in common metabolomic and proteomic applications.
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors: Hoi Sze
authors: HS
authors: Yeung
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.|‡Shandong Academy of Sciences, Jinan, Shandong, P. R. China.
authors: Xiangfeng
authors: X
authors: Chen
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors: Wan
authors: W
authors: Li
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors: Ze
authors: Z
authors: Wang
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors: Y L Elaine
authors: YL
authors: Wong
authors: †Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China.
authors: T-W Dominic
authors: TW
authors: Chan
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006801:Humans; D008567:Membranes, Artificial; D008904:Miniaturization / Q000379:methods*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000097:blood / Q000302:isolation & purification; D010748:Phosphopeptides / Q000737:chemistry; D040901:Proteomics; D011759:Pyrrolidines / Q000097:blood / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods*; D014025:Titanium / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism; D000077597:Vildagliptin
chemical_list: D008567:Membranes, Artificial; D009570:Nitriles; D010748:Phosphopeptides; D011759:Pyrrolidines; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium; D014357:Trypsin; D000077597:Vildagliptin; D000218:Adamantane