title: Microglial content-dependent inhibitory effects of calcitonin gene-related peptide (CGRP) on murine retroviral infection of glial cells.
abstract: C57BL/6 (B6) mice develop peripheral neuropathy post-LP-BM5 infection, a murine model of HIV-1 infection, along with the up-regulation of select spinal cord cytokines. We investigated if calcitonin gene-related peptide (CGRP) contributed to the development of peripheral neuropathy by stimulating glial responses. An increased expression of lumbar spinal cord CGRP was observed in vivo, post-LP-BM5 infection. Consequently, in vitro CGRP co-treatments led to a microglial content-dependent attenuation of viral loads in spinal cord mixed glia infected with selected doses of LP-BM5. This inhibition was neither caused by the loss of glia nor induced via the direct inhibition of LP-BM5 by CGRP.
authors: Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME.
authors: Jennifer
authors: J
authors: Malon
authors: Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME.
authors: Eliza
authors: E
authors: Grlickova-Duzevik
authors: Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME.
authors: James
authors: J
authors: Vaughn
authors: Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME.
authors: Holly
authors: H
authors: Beaulac
authors: College of Arts and Sciences, University of New England, Biddeford, ME.
authors: Tyler R
authors: TR
authors: Vunk
authors: Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME.
authors: Ling
authors: L
authors: Cao
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism* / Q000494:pharmacology; D002452:Cell Count; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000378:metabolism*; D012190:Retroviridae / Q000472:pathogenicity; D012192:Retroviridae Infections / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology*; D015854:Up-Regulation / Q000502:physiology*; D010948:Viral Plaque Assay
chemical_list: D016207:Cytokines; D015740:Calcitonin Gene-Related Peptide
keywords: Calcitonin gene-related peptide (CGRP); Glia; LP-BM5; Murine acquired immunodeficiency syndrome (MAIDS); Peripheral neuropathy; Retroviral infection