title: Genome-wide analysis and expression of the calcium-dependent protein kinase gene family in cucumber.
abstract: Calcium-dependent protein kinases (CDPKs) are multi-functional proteins that combine calcium-binding and signaling capabilities within a single gene product. Current studies have shown that the CDPKs regulate numerous growth and developmental processes and biotic and abiotic stress responses. Nonetheless, knowledge concerning the specific expression patterns and evolutionary history of the CDPK family in cucumber (Cucumis sativus L.) remains very limited. We, therefore, investigated the phylogenetic relationships and expression profiles of the 19 CDPK genes identified in the cucumber genome sequence, resolving them into four subfamilies based on a phylogenetic tree and gene structures. Tissue-specific expression profiles suggest that cucumber CDPK genes are involved in cucumber tissue development. An expression analysis based on qRT-PCR indicated that cucumber CDPK genes are extensively involved in abscisic acid, salt, cold, drought, heat, and waterlogging responses, possibly by different mechanisms. The fates of two paralogs after divergence were also investigated, suggesting subfunctionalization and neofunctionalization during evolution. These observations lay an important foundation for functional and evolutionary analyses of the CDPK gene family in cucurbitaceae species.
authors: School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors: Xuewen
authors: X
authors: Xu
authors: Min
authors: M
authors: Liu
authors: Lu
authors: L
authors: Lu
authors: Min
authors: M
authors: He
authors: Wenqin
authors: W
authors: Qu
authors: Qiang
authors: Q
authors: Xu
authors: Xiaohua
authors: X
authors: Qi
authors: Xuehao
authors: X
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D016000:Cluster Analysis; D018553:Cucumis sativus / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011494:Protein Kinases / Q000145:classification / Q000235:genetics*; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases