title: In vivo therapeutic potential of mesenchymal stromal cells depends on the source and the isolation procedure.
abstract: Over the last several years, mesenchymal stromal cells (MSCs) have been isolated from different tissues following a variety of different procedures. Here, we comparatively assess the ex vivo and in vivo properties of MSCs isolated from either adipose tissue or bone marrow by different purification protocols. After MSC transplantation into a mouse model of hindlimb ischemia, clinical and histological analysis revealed that bone marrow MSCs purified on adhesive substrates exerted the best therapeutic activity, preserving tissue viability and promoting formation of new arterioles without directly transdifferentiating into vascular cells. In keeping with these observations, these cells abundantly expressed cytokines involved in vessel maturation and cell retention. These findings indicate that the choice of MSC source and purification protocol is critical in determining the therapeutic potential of these cells and warrant the standardization of an optimal MSC isolation procedure in order to select the best conditions to move forward to more effective clinical experimentation.
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy.
authors: Francesca
authors: F
authors: Bortolotti
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy.
authors: Laura
authors: L
authors: Ukovich
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy; Tissue Engineering Department, Shiraz University of Medical Sciences, School of Advanced Medical Sciences and Technologies, Shiraz 7134814336, Iran.
authors: Vahid
authors: V
authors: Razban
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy.
authors: Valentina
authors: V
authors: Martinelli
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy.
authors: Giulia
authors: G
authors: Ruozi
authors: Struttura Complessa di Medicina Nucleare, "Ospedali Riuniti," 34142 Trieste, Italy.
authors: Barbara
authors: B
authors: Pelos
authors: Struttura Complessa di Medicina Nucleare, "Ospedali Riuniti," 34142 Trieste, Italy.
authors: Franca
authors: F
authors: Dore
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy.
authors: Mauro
authors: M
authors: Giacca
authors: Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), 34149 Trieste, Italy. Electronic address: zacchign@icgeb.org.
authors: Serena
authors: S
authors: Zacchigna
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006614:Hindlimb / Q000098:blood supply / Q000473:pathology; D016130:Immunophenotyping; D007511:Ischemia / Q000473:pathology / Q000628:therapy; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D018919:Neovascularization, Physiologic; D010641:Phenotype