title: Vascular endothelial injury and apoptosis in rats with severe acute pancreatitis.
abstract: We explored mechanisms of vascular endothelial injury that lead to systemic multiple organ failure by detecting the soluble endothelial protein C receptor (sEPCR), von Willebrand factor (vWF), serum nitric oxide (NO), and tumor necrosis factor alpha (TNF-α) and Bcl-2 mRNA and Bax mRNA expression in a severe acute pancreatitis (SAP) rat model. Compared to controls, the levels of TNF-α, vWF, and sEPCR were significantly increased in the experimental group at 12 hours and 24 hours and the NO level was significantly decreased. After 12 hours, the aortic endothelial apoptosis index and Bax mRNA expression in aortic endothelial cells had increased in the experimental group, but Bcl-2 mRNA levels had decreased. All these changes appeared at both 12 h and 24 hours. The results indicated that vascular endothelial injury and apoptosis markers were elevated in SAP. Endothelial injury and increased apoptosis in the experimental group were related to the increased expression of TNF-α.
authors: Division of Geriatric Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors: Ning
authors: N
authors: Ge
authors: Chinese Medicine Department, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors: Qing
authors: Q
authors: Xia
authors: Division of Geriatric Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors: Zhong-Hua
authors: ZH
authors: Yang
authors: Division of Geriatric Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors: Qun-Fang
authors: QF
authors: Ding
authors: Cardiac Medicine Department, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors: Zhi
authors: Z
authors: Zeng