title: Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage JS01.
abstract: Staphylococcus aureus is a primary pathogen that causes bovine mastitis resulting in serious economic losses and herd management problems in dairy cows. A novel bacteriophage, JS01, specifically infecting bovine S. aureus, was isolated from milk of mastitis-affected cattle. TEM observation showed that it belonged to the family Siphovirus. The JS01 strain demonstrated a broad host range. The prediction result of PHACTS suggested that the JS01 strain was temperate phage. The JS01 genome is 43,458 bp long, with a GC content of 33.32% and no tRNAs. Annotation and functional analysis of the predicted ORFs revealed six functional groups: structure and morphology, DNA replication and regulation, packaging, lysogeny, lysis, and pathogenicity. Comparative analysis between JS01, S. aureus MSSA476, and S. aureus prophage PVL was also performed. The characterization and genomic analysis of JS01 provide a better understanding of S. aureus-targeting bacteriophages and useful information for the development of phage-based biocontrol agents against S. aureus.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Hongying
authors: H
authors: Jia
authors: Wenyang
authors: W
authors: Dong
authors: Lvfeng
authors: L
authors: Yuan
authors: Jiale
authors: J
authors: Ma
authors: Qinqin
authors: Q
authors: Bai
authors: Zihao
authors: Z
authors: Pan
authors: Chengping
authors: C
authors: Lu
authors: Huochun
authors: H
authors: Yao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005260:Female; D016679:Genome, Viral; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013204:Staphylococcus Phages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000821:virology*