title: In vitro evaluation of celluloytic Bacillus amyloliquefaciens AMS1 isolated from traditional fermented soybean (Churpi) as an animal probiotic.
abstract: A microorganism showing probiotic attributes and hydrolyzing carboxymethylcellulose was isolated from traditional fermented soybean (Churpi) and identified as Bacillus amyloliquefaciens by analysis of 16S rRNA gene sequence and named as B. amyloliquefaciens AMS1. The potentiality of this isolate as probiotic was investigated in vitro and it showed gastrointestinal transit tolerance, cell surface hydrophobicity, cell aggregation and antimicrobial activity. The isolate was found to be non-hemolytic which further strengthens its candidature as a potential probiotic. The maize straw digestion was confirmed by scanning electron microscopy studies. The isolate was able to degrade filter paper within 96 hours of incubation. This study explores the possibility of combining the cellulase degrading ability of a microbe with its probiotic attributes to enhance gut health of animal and digestibility of the feed.
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Ajay K
authors: AK
authors: Manhar
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Devabrata
authors: D
authors: Saikia
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Yasir
authors: Y
authors: Bashir
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Reba K
authors: RK
authors: Mech
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Dhrubajyoti
authors: D
authors: Nath
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.
authors: Bolin K
authors: BK
authors: Konwar
authors: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India. Electronic address: mandal@tezu.ernet.in.
authors: Manabendra
authors: M
authors: Mandal
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D005285:Fermentation; D008969:Molecular Sequence Data; D010802:Phylogeny; D019936:Probiotics / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Animal feed; Bacillus amyloliquefaciens; Cellulolytic; Probiotic; Soybean