title: A manual and an automatic TERS based virus discrimination.
abstract: Rapid techniques for virus identification are more relevant today than ever. Conventional virus detection and identification strategies generally rest upon various microbiological methods and genomic approaches, which are not suited for the analysis of single virus particles. In contrast, the highly sensitive spectroscopic technique tip-enhanced Raman spectroscopy (TERS) allows the characterisation of biological nano-structures like virions on a single-particle level. In this study, the feasibility of TERS in combination with chemometrics to discriminate two pathogenic viruses, Varicella-zoster virus (VZV) and Porcine teschovirus (PTV), was investigated. In a first step, chemometric methods transformed the spectral data in such a way that a rapid visual discrimination of the two examined viruses was enabled. In a further step, these methods were utilised to perform an automatic quality rating of the measured spectra. Spectra that passed this test were eventually used to calculate a classification model, through which a successful discrimination of the two viral species based on TERS spectra of single virus particles was also realised with a classification accuracy of 91%.
authors: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Helmholtzweg 4, 07743 Jena, Germany. juergen.popp@ipht-jena.de.
authors: Konstanze
authors: K
authors: Olschewski
authors: Evelyn
authors: E
authors: Kämmer
authors: Stephan
authors: S
authors: Stöckel
authors: Thomas
authors: T
authors: Bocklitz
authors: Tanja
authors: T
authors: Deckert-Gaudig
authors: Roland
authors: R
authors: Zell
authors: Dana
authors: D
authors: Cialla-May
authors: Karina
authors: K
authors: Weber
authors: Volker
authors: V
authors: Deckert
authors: Jürgen
authors: J
authors: Popp
mesh_terms: D000818:Animals; D014645:Herpesvirus 3, Human / Q000737:chemistry*; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013552:Swine; D052700:Teschovirus / Q000737:chemistry*