title: Extracorporeal photopheresis promotes IL-1β production.
abstract: Extracorporeal photopheresis (ECP) is a widely used clinical cell-based therapy exhibiting efficacy in heterogenous immune-mediated diseases such as cutaneous T cell lymphoma, graft-versus-host disease, and organ allograft rejection. Despite its documented efficacy in cancer immunotherapy, little is known regarding the induction of immunostimulatory mediators by ECP. In this article, we show that ECP promotes marked release of the prototypic immunostimulatory cytokine IL-1β. ECP primes IL-1β production and activates IL-1β maturation and release in the context of caspase-1 activation in monocytes and myeloid dendritic cells. Of interest, IL-1β maturation by ECP was fully intact in murine cells deficient in caspase-1, suggesting the predominance of an inflammasome-independent pathway for ECP-dependent IL-1β maturation. Clinically, patient analysis revealed significantly increased IL-1β production in stimulated leukapheresis concentrates and peripheral blood samples after ECP. Collectively, these results provide evidence for promotion of IL-1β production by ECP and offer new insight into the immunostimulatory capacity of ECP.
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany;
authors: Erhan
authors: E
authors: Yakut
authors: Institute of Molecular Medicine, University Hospital, University of Bonn, 53127 Bonn, Germany; and.
authors: Christopher
authors: C
authors: Jakobs
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany;
authors: Adriana
authors: A
authors: Peric
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany;
authors: Gabriela
authors: G
authors: Michel
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany;
authors: Nelli
authors: N
authors: Baal
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany;
authors: Gregor
authors: G
authors: Bein
authors: Institute of Biochemistry I, Faculty of Medicine, Goethe-University Frankfurt, 60590 Frankfurt, Germany.
authors: Bernhard
authors: B
authors: Brüne
authors: Institute of Molecular Medicine, University Hospital, University of Bonn, 53127 Bonn, Germany; and.
authors: Veit
authors: V
authors: Hornung
authors: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, D-35390 Giessen, Germany; Holger.Hackstein@immunologie.med.uni-giessen.de.
authors: Holger
authors: H
authors: Hackstein
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D058847:Inflammasomes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008730:Methoxsalen / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D017893:Photopheresis / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D058847:Inflammasomes; D053583:Interleukin-1beta; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D017319:Photosensitizing Agents; D000255:Adenosine Triphosphate; D020170:Caspase 1; D008730:Methoxsalen