title: Expression of porcine protegrin-1 inPichia pastorisand its anticancer activityin vitro.
abstract: Protegrin-1 (PG-1), a β-hairpin antimicrobial peptide (AMP), is amongst the shortest AMPs in sequence length while remaining active against a variety of microorganisms. The aim of this study was produce recombinant PG-1 and investigate its anticancer activity. A DNA sequence encoding the mature PG-1, fused with a 6His-tag, was cloned into the pPICZα-A vector and transformed intoPichia pastoris. Expression was induced following culture for ~96 h with 1% methanol at 28°C, and ~15.6 mg PG-1 was expressed in 100 ml culture medium. Following purification using a Ni-chelating Sepharose column, ~20 mg pure active PG-1 was obtained from 500 ml culture broth supernatant. The expressed PG-1/6His exhibited strong dose- and time-dependent anticancer activity against HepG2 cellsin vitro.
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Mingfu
authors: M
authors: Niu
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Shumao
authors: S
authors: Chai
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Xiaoyan
authors: X
authors: You
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Wenhui
authors: W
authors: Wang
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Cuili
authors: C
authors: Qin
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Qiang
authors: Q
authors: Gong
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Tingting
authors: T
authors: Zhang
authors: Food and Bioengineering College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors: Peng
authors: P
authors: Wan
keywords: antibacterial peptide protegrin-1/His; anticancer activity; expression and purification