title: Sandwich enzyme-linked immunosorbent assay for the quantification of human serum albumin fragment 408-423 in bodily fluids.
abstract: Urinary levels of human serum albumin (hSA) fragment 408-423 have been proposed to represent an early marker for graft-versus-host disease (GvHD) and chronic kidney diseases. Here, we developed an enzyme-linked immunosorbent assay (ELISA) for the quantification of hSA(408-423). The sandwich ELISA has a detection limit of 0.5ng/ml and is highly specific for hSA(408-423) because it does not cross-react with other albumin fragments or the full-length precursor. This ELISA allows rapid and convenient quantification of hSA(408-423) in bodily fluids, further clarifying the prognostic and diagnostic value of this peptide in GvHD, kidney disease, and other disorders.
authors: Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; International Graduate School in Molecular Medicine Ulm, Ulm University, 89081 Ulm, Germany.
authors: Katharina B
authors: KB
authors: Mohr
authors: Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.
authors: Onofrio
authors: O
authors: Zirafi
authors: ELISA-Entwicklung, 53359 Rheinbach, Germany.
authors: Mark
authors: M
authors: Hennies
authors: Core Unit Mass Spectrometry and Proteomics, Ulm University, 89081 Ulm, Germany.
authors: Sebastian
authors: S
authors: Wiese
authors: Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Ulm Peptide Pharmaceuticals, Ulm University, 89081 Ulm, Germany.
authors: Frank
authors: F
authors: Kirchhoff
authors: Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Ulm Peptide Pharmaceuticals, Ulm University, 89081 Ulm, Germany. Electronic address: jan.muench@uni-ulm.de.
authors: Jan
authors: J
authors: Münch
mesh_terms: D000418:Albumins / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006086:Graft vs Host Disease / Q000378:metabolism*; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*
chemical_list: D000418:Albumins; D012709:Serum Albumin
keywords: Biomarker; ELISA; Graft-versus-host disease; Human serum albumin; Kidney disease; Peptide