title: Diagnostic challenges of tuberculous lymphadenitis using polymerase chain reaction analysis: a case study.
abstract: This report presents a case of tuberculous lymphadenitis that was difficult to diagnose using polymerase chain reaction analysis. An 80-year-old Japanese female was hospitalized due to swollen cervical lymph nodes. Her lymph node tests revealed paradoxical polymerase chain reaction results. Polymerase chain reaction analysis of two biopsy tissues using the Cobas TaqMan revealed a positive result for Mycobacterium avium and a negative result for Mycobacterium tuberculosis. However, polymerase chain reaction analysis of a cultured colony of acid-fast bacteria from biopsy tissue using the Cobas TaqMan and an alternative polymerase chain reaction analysis of biopsy tissue yielded discordant results. The patient was diagnosed as having tuberculous lymphadenitis. She was treated with antitubercular drugs and subsequently had a reduction in cervical lymph node swelling. Polymerase chain reaction analysis is not 100% accurate; hence, its use as a diagnostic tool for mycobacterial infection requires increased attention.
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Hirokazu
authors: H
authors: Taniguchi
authors: The Division of Microbiology, Department of Medical Laboratory, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Masahiko
authors: 0000-0002-0695-1486
authors: M
authors: Nakamura
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Kazuki
authors: 0000-0002-6330-4533
authors: K
authors: Shimokawa
authors: The Department of Otolaryngology, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Fumi
authors: F
authors: Kamiseki
authors: The Department of Pathology, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Shin
authors: S
authors: Ishizawa
authors: The Department of Diagnostic Radiology, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Hitoshi
authors: H
authors: Abo
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Hideaki
authors: H
authors: Furuse
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Takeshi
authors: 0000-0002-6243-4459
authors: T
authors: Tsuda
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Yasuaki
authors: 0000-0001-7001-9725
authors: Y
authors: Masaki
authors: The Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama 930-8550, Japan.
authors: Kensuke
authors: K
authors: Suzuki