title: Codon optimization of genes for efficient protein expression in mammalian cells by selection of only preferred human codons.
abstract: A simple design method for codon optimization of genes to express a heterologous protein in mammalian cells is described. Codon optimization was performed by choosing only codons preferentially used in humans and with over 60% GC content, and the method was named the "preferred human codon-optimized method." To test our simple rule for codon optimization, the preferred human codon-optimized genes for six proteins containing photoproteins (aequorin and clytin II) and luciferases (Gaussia luciferase, Renilla luciferase, and firefly luciferases from Photinus pyralis and Luciola cruciata) were chemically synthesized and transiently expressed in Chinese hamster ovary-K1 cells. All preferred human codon-optimized genes showed higher luminescence activity than the corresponding wild-type genes. Our simple design method could be used to improve protein expression in mammalian cells efficiently.
authors: Yokohama Research Center, JNC Co., 5-1 Okawa, Kanazawa-ku, Yokohama 236-8605, Japan. Electronic address: sinouye@jnc-corp.co.jp.
authors: Satoshi
authors: S
authors: Inouye
authors: Yokohama Research Center, JNC Co., 5-1 Okawa, Kanazawa-ku, Yokohama 236-8605, Japan.
authors: Yuiko
authors: Y
authors: Sahara-Miura
authors: Yokohama Research Center, JNC Co., 5-1 Okawa, Kanazawa-ku, Yokohama 236-8605, Japan.
authors: Jun-ichi
authors: J
authors: Sato
authors: Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors: Takahiro
authors: T
authors: Suzuki
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D003062:Codon / Q000235:genetics*; D005796:Genes; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D008322:Mammals; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D003062:Codon; D010750:Phosphoproteins; D011506:Proteins; D008156:Luciferases
keywords: Codon optimization; Luciferase; Photoprotein; Reporter gene; Synthetic gene