title: Transgenic sugarcane resistant to Sorghum mosaic virus based on coat protein gene silencing by RNA interference.
abstract: As one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. It is mainly caused by Potyvirus sugarcane mosaic virus (SCMV) and/or Sorghum mosaic virus (SrMV), with additional differences in viral strains. RNA interference (RNAi) is a novel strategy for producing viral resistant plants. In this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of SrMV, the conserved region of coat protein (CP) genes was selected as the target gene and the interference sequence with size of 423 bp in length was obtained through PCR amplification. The RNAi vector pGII00-HACP with an expression cassette containing both hairpin interference sequence and cp4-epsps herbicide-tolerant gene was transferred to sugarcane cultivar ROC22 via Agrobacterium-mediated transformation. After herbicide screening, PCR molecular identification, and artificial inoculation challenge, anti-SrMV positive transgenic lines were successfully obtained. SrMV resistance rate of the transgenic lines with the interference sequence was 87.5% based on SrMV challenge by artificial inoculation. The genetically modified SrMV-resistant lines of cultivar ROC22 provide resistant germplasm for breeding lines and can also serve as resistant lines having the same genetic background for study of resistance mechanisms.
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Jinlong
authors: J
authors: Guo
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Shiwu
authors: S
authors: Gao
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Qinliang
authors: Q
authors: Lin
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Hengbo
authors: H
authors: Wang
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Youxiong
authors: Y
authors: Que
authors: Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; National Research and Development Center for Sugarcane Industry Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Liping
authors: L
authors: Xu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D009029:Mosaic Viruses / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D034622:RNA Interference; D031786:Saccharum / Q000235:genetics* / Q000378:metabolism / Q000821:virology
chemical_list: D036022:Capsid Proteins