title: Illumina sequencing-based analysis of free-living bacterial community dynamics during an Akashiwo sanguine bloom in Xiamen sea, China.
abstract: Although phytoplankton are the major source of marine dissolved organic matter (DOM), their blooms are a global problem that can greatly affect marine ecological systems, especially free-living bacteria, which are the primary DOM degraders. In this study, we analyzed free-living bacterial communities from Xiamen sea during an Akashiwo sanguine bloom using Illumina MiSeq sequencing of 16S rRNA gene amplicons. The bloom was probably stimulated by low salinity and ended after abatement of eutrophication pollution. A total of 658,446 sequence reads and 11,807 OTUs were obtained in both bloom and control samples with Alpha-proteobacteria and Gamma-proteobacteria being the predominant classes detected. The bloom decreased bacterial diversity, increased species evenness, and significantly changed the bacterial community structure. Bacterial communities within the bloom were more homogeneous than those within the control area. The bacteria stimulated by this bloom included the SAR86 and SAR116 clades and the AEGEAN-169 marine group, but a few were suppressed. In addition, many bacteria known to be associated with phytoplankton were detected only in the bloom samples. This study revealed the great influence of an A. sanguinea bloom on free-living bacterial communities, and provided new insights into the relationship between bacteria and A. sanguinea in marine ecosystems.
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Caiyun
authors: C
authors: Yang
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Yi
authors: Y
authors: Li
authors: Department of Computer Science, Stanford University, Stanford, California 94305, USA.
authors: Benjamin
authors: B
authors: Zhou
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Yanyan
authors: Y
authors: Zhou
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Wei
authors: W
authors: Zheng
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Yun
authors: Y
authors: Tian
authors: Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 730722, USA.
authors: Joy D
authors: JD
authors: Van Nostrand
authors: Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 730722, USA.
authors: Liyou
authors: L
authors: Wu
authors: Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 730722, USA.
authors: Zhili
authors: Z
authors: He
authors: 1] Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 730722, USA [2] Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA [3] School of Environment, Tsinghua University, Beijing 100084, China.
authors: Jizhong
authors: J
authors: Zhou
authors: State Key Laboratory for Marine Environmental Science, and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Tianling
authors: T
authors: Zheng
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D004141:Dinoflagellida / Q000254:growth & development* / Q000382:microbiology; D020563:Gammaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012623:Seawater / Q000382:microbiology*; D017423:Sequence Analysis, RNA
chemical_list: D012336:RNA, Ribosomal, 16S