title: Selenium speciation in tea by dispersive liquid-liquid microextraction coupled to high-performance liquid chromatography after derivatization with 2,3-diaminonaphthalene.
abstract: Selenium is an important element for human health, and it is present in many natural drinks and foods. Present study described a new method using dispersive liquid-liquid microextraction prior to high-performance liquid chromatography with a UV variable wavelength detector for the determination of the total selenium, Se(IV), Se(VI), and total organoselenium in tea samples. In the procedure, 2,3-diaminonaphthalene was used as the chelating reagent, 400 μL acetonitrile was used as the disperser solvent and 60 μL chlorobenzene was used as the extraction solvent. The complex of Se(IV) and 2,3-diaminonaphthalene in the final extracted phase was analyzed by high-performance liquid chromatography. The factors influencing the derivatization and microextraction were investigated. Under the optimal conditions, the limit of detection was 0.11 μg/L for Se(IV) and the linearity range was in the range of 0.5-40 μg/L. This method was successfully applied to the determination of selenium in four tea samples with spiked recoveries ranging from 91.3 to 100%.
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Qingxiang
authors: Q
authors: Zhou
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Man
authors: M
authors: Lei
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Jing
authors: J
authors: Li
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Mengyun
authors: M
authors: Wang
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Danchen
authors: D
authors: Zhao
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: An
authors: A
authors: Xing
authors: Beijing Key Laboratory of Oil and Gas Pollution Control, College of Geosciences, China University of Petroleum Beijing, Beijing, China.
authors: Kuifu
authors: K
authors: Zhao
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D059627:Liquid Phase Microextraction; D016566:Organoselenium Compounds / Q000032:analysis*; D012643:Selenium / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D016566:Organoselenium Compounds; D013662:Tea; C026373:2,3-diaminonaphthalene; D015081:2-Naphthylamine; D012643:Selenium
keywords: 2,3-Diaminonaphthalene; Dispersive liquid-liquid microextraction; Selenium; Tea