title: Immobilized lipase from Schizophyllum commune ISTL04 for the production of fatty acids methyl esters from cyanobacterial oil.
abstract: Novel lipase from model mushroom Schizophyllum commune strain ISTL04 produced by solid state fermentation of Leucaena leucocephala seeds, was immobilized onto Celite for enzymatic FAMEs production from cyanobacterial endolith Leptolyngbya ISTCY101. The isolate showed vigorous growth and produced remarkable lipase activity of 146.5 U g(-1) dry solid substrate, without any external lipase inducer. Single-factor experiments were carried out to study the effects of various reaction parameters on the FAMEs yield. The best conditions for enzymatic transesterification as revealed by the results were: 1:3 oil to methanol molar ratio, added at 3h intervals, 12% water content, 1581.5 U g(-1) immobilized lipase, temperature 45 °C, and time 24h. Under these conditions, the maximum FAMEs yield reached 94%. The immobilized lipase was able to produce >90% of the relative FAMEs yield after four repeated transesterification cycles. This immobilized lipase exhibited potential for application in biodiesel industry.
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors: Jyoti
authors: J
authors: Singh
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors: Manoj Kumar
authors: MK
authors: Singh
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors: Madan
authors: M
authors: Kumar
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: isthakur@hotmail.com.
authors: Indu Shekhar
authors: IS
authors: Thakur
mesh_terms: D000363:Agaricales / Q000201:enzymology; D056804:Biofuels; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry; D007692:Diatomaceous Earth / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004951:Esterification; D004952:Esters / Q000737:chemistry*; D005227:Fatty Acids / Q000096:biosynthesis*; D005285:Fermentation; D007218:Industrial Microbiology; D008049:Lipase / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D012567:Schizophyllum / Q000201:enzymology*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D009821:Oils; D014867:Water; D007692:Diatomaceous Earth; D008049:Lipase; D000432:Methanol
keywords: Biodiesel; Celite; FAMEs; Immobilized lipase; Schizophyllum commune