title: Structural and genetic studies of the O-antigen of Escherichia coli O163.
abstract: An acidic O-polysaccharide (O-antigen) of Escherichia coli O163 was obtained by mild acid hydrolysis of the lipopolysaccharide and studied by sugar analysis and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear tetrasaccharide repeating unit was established, which is unique among known structures of bacterial polysaccharides: -->2)-β-D-Manp-(1-->4)-β-D-GlcpA-(1-->3)-α-L-QuipNAc-(1-->3)-α-D-GlcpNAc-(1-->. Functions of genes in the O-antigen gene cluster of E. coli O163 were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure. Relationships between O-antigen structures and gene clusters of E. coli O163 and Salmonella enterica O41 are discussed.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors: Andrei V
authors: AV
authors: Perepelov
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors: Quan
authors: Q
authors: Wang
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Sof'ya N
authors: SN
authors: Senchenkova
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors: Ye
authors: Y
authors: Qian
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Alexander S
authors: AS
authors: Shashkov
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors: Lei
authors: L
authors: Wang
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D016366:Open Reading Frames
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-Antigen; O-Antigen gene cluster