title: Effects of prior treatment with simvastatin on skeletal muscle structure and mitochondrial enzyme activities during early phases of sepsis.
abstract: We investigated the effects of the early phase of sepsis and prior treatment of Simvastatin on muscle structure and mitochondrial enzymes treated with lipopolysaccharide (LPS) in rats. We divided rats into control, LPS, simvastatin, simvastatin + LPS groups. Mitochondrial citrate synthase, complex I, II, I + III, II + III, cytochrome c oxidase (COX) activities were measured. Muscle tissue was stained using modified Gomori trichrome (MGT), succinic dehydrogenase (SDH) and cytochrome oxidase (COX). In all treated groups, complex I and citrate synthase activities were higher than in the controls. In the control and LPS groups, COX activity was increased when compared with simvastatins'. Complex II, II-III activities were higher in the LPS group than in the control group. Complex I-III activities were higher in the Simvastatin and Simvastatin + LPS groups than in the control and LPS groups (P < 0.05). Myopathic changes with LPS group were observed in MGT stained sections. Our findings showed improvements in the alterations of enzyme activities and muscle myofibrils after treating rats with LPS that had received a prior dose of simvastatin.
authors: Deparment of Neuroscience, The Institute for Experimental Medicine, Istanbul University Istanbul, Turkey.
authors: Elif
authors: E
authors: Ozkok
authors: School of Nursing, Halic University Istanbul, Turkey.
authors: Hatice
authors: H
authors: Yorulmaz
authors: Department of Physiology, Istanbul Medical Faculty, Istanbul University Istanbul, Turkey.
authors: Gulten
authors: G
authors: Ates
authors: Department of Neurology/Neuromuscular Disorders Unit, Istanbul Faculty of Medicine, Istanbul University Istanbul, Turkey.
authors: Piraye
authors: P
authors: Serdaroglu-Oflazer
authors: Department of Physiology, Istanbul Medical Faculty, Istanbul University Istanbul, Turkey.
authors: Ayse Sule
authors: AS
authors: Tamer
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000139:chemically induced / Q000473:pathology*; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008070:Lipopolysaccharides; D019821:Simvastatin
keywords: Lipopolysaccharide; mitochondrial enzyme activites; rat; simvastatin; skeletal muscle