title: Somatic copy number alterations associated with Japanese or endometriosis in ovarian clear cell adenocarcinoma.
abstract: When compared with other epithelial ovarian cancers, the clinical characteristics of ovarian clear cell adenocarcinoma (CCC) include 1) a higher incidence among Japanese, 2) an association with endometriosis, 3) poor prognosis in advanced stages, and 4) a higher incidence of thrombosis as a complication. We used high resolution comparative genomic hybridization (CGH) to identify somatic copy number alterations (SCNAs) associated with each of these clinical characteristics of CCC. The Human Genome CGH 244A Oligo Microarray was used to examine 144 samples obtained from 120 Japanese, 15 Korean, and nine German patients with CCC. The entire 8q chromosome (minimum corrected p-value: q = 0.0001) and chromosome 20q13.2 including the ZNF217 locus (q = 0.0078) were amplified significantly more in Japanese than in Korean or German samples. This copy number amplification of the ZNF217 gene was confirmed by quantitative real-time polymerase chain reaction (Q-PCR). ZNF217 RNA levels were also higher in Japanese tumor samples than in non-Japanese samples (P = 0.027). Moreover, endometriosis was associated with amplification of EGFR gene (q = 0.047), which was again confirmed by Q-PCR and correlated with EGFR RNA expression. However, no SCNAs were significantly associated with prognosis or thrombosis. These results indicated that there may be an association between CCC and ZNF217 amplification among Japanese patients as well as between endometriosis and EGFR gene amplifications.
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Aikou
authors: A
authors: Okamoto
authors: Department of Obstetrics and Gynecology, Charité University Hospital, Berlin, Germany.
authors: Jalid
authors: J
authors: Sehouli
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Nozomu
authors: N
authors: Yanaihara
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Yukihiro
authors: Y
authors: Hirata
authors: Department of Obstetrics and Gynecology, Charité University Hospital, Berlin, Germany.
authors: Ioana
authors: I
authors: Braicu
authors: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors: Byoung-Gie
authors: BG
authors: Kim
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Takakura
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Misato
authors: M
authors: Saito
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Yanagida
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Masataka
authors: M
authors: Takenaka
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Noriko
authors: N
authors: Yamaguchi
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Asuka
authors: A
authors: Morikawa
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Tanabe
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Kyosuke
authors: K
authors: Yamada
authors: Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Kosuke
authors: K
authors: Yoshihara
authors: Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Takayuki
authors: T
authors: Enomoto
authors: Department of Obstetrics and Gynecology, Tottori University School of Medicine, Yonago, Japan.
authors: Hiroaki
authors: H
authors: Itamochi
authors: Department of Obstetrics and Gynecology, Tottori University School of Medicine, Yonago, Japan.
authors: Junzo
authors: J
authors: Kigawa
authors: Department of Obstetrics and Gynecology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors: Noriomi
authors: N
authors: Matsumura
authors: Department of Obstetrics and Gynecology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors: Ikuo
authors: I
authors: Konishi
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Aida
authors: Pharmaceutical Research Department 2, Research Division, Chugai Pharmaceutical Co., Ltd., Kamakura, Japan.
authors: Yuko
authors: Y
authors: Aoki
authors: Pharmaceutical Research Department 2, Research Division, Chugai Pharmaceutical Co., Ltd., Kamakura, Japan.
authors: Nobuya
authors: N
authors: Ishii
authors: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
authors: Kazunori
authors: K
authors: Ochiai
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
authors: Tetsu
authors: T
authors: Akiyama
authors: Division of Molecular Epidemiology, Jikei University School of Medicine, Tokyo, Japan.
authors: Mitsuyoshi
authors: M
authors: Urashima
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000628:therapy; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D002869:Chromosome Aberrations; D002890:Chromosomes, Human, Pair 20; D002898:Chromosomes, Human, Pair 8; D055028:Comparative Genomic Hybridization; D004715:Endometriosis / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000628:therapy; D060888:Real-Time Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics
chemical_list: D015534:Trans-Activators; C113698:ZNF217 protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors