title: Attenuated Salmonella enterica Typhimurium reduces tumor burden in an autochthonous breast cancer model.
abstract: OBJECTIVE
  Cancer treatment with attenuated Salmonella enterica Typhimurium (S. Typhimurium) has gained momentum in recent years. However, the effectiveness of this treatment has not been explored in autochthonous models. We report the efficacy of S. Typhimurium in mice with autochthonous mammary tumors.
  
  
  METHODS
  S. Typhimurium attenuated by deletion of cyclic adenosine monophosphate signaling, SalpNG.1, was injected into female BALB-neuT tumor-bearing mice. Mice were monitored for efficacy and sacrificed for mechanistic studies.
  
  
  RESULTS
  In treated mice, seven-week post-treatment tumor burden was reduced by 85% and median survival was increased by 88%. Efficacy was correlated with increased tumor-infiltrating CD8 and natural killer cells. In addition, SalpNG.1 treatment caused a systemic increase of monocytic myeloid-derived suppressor cells that accumulated to high numbers within tumor tissue. Bacteria were not detected in tumor tissue, suggesting that the observed efficacy was due to a systemic rather than a tumor-specific effect of the bacteria.
  
  
  CONCLUSIONS
  S. Typhimurium treatment reduces tumor burden and increases survival in an autochthonous breast cancer model.
authors: Department of Surgery, University of Minnesota, Minneapolis, MN, U.S.A. dree0036@umn.edu.
authors: Jeremy
authors: J
authors: Drees
authors: Department of Surgery, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Michael
authors: M
authors: Mertensotto
authors: Department of Pharmaceutics, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Garvey
authors: G
authors: Liu
authors: Department of Pharmaceutics, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Jayanth
authors: J
authors: Panyam
authors: Department of Surgery, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Arnold
authors: A
authors: Leonard
authors: Department of Surgery, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Lance
authors: L
authors: Augustin
authors: Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Janet
authors: J
authors: Schottel
authors: Department of Surgery, University of Minnesota, Minneapolis, MN, U.S.A.
authors: Daniel
authors: D
authors: Saltzman
mesh_terms: D000818:Animals; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012486:Salmonella typhimurium / Q000502:physiology*; D047368:Tumor Burden
keywords: BALB-neuT; Salmonella; autochthonous model; breast cancer; cancer therapy; genetically-engineered; immunotherapy; mouse model; myeloid-derived suppressor cells