title: The benefit of a human bone marrow stem cells concentrate in addition to an inorganic scaffold for bone regeneration: an in vitro study.
abstract: Background. This work compares the osteoblastic behaviour of a bone marrow (BM) aspirate and a prepared BM concentrate of nucleated cells associated with a glass reinforced hydroxyapatite composite (GRHC) in a microporous pellet formulation. Methods. BM aspirate (30 mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3 mL) from the BM aspirates. The BM aspirates (53% cell viability; 2.7 × 10(6) nucleated cell/mL) and the BM concentrates (76% cell viability; 2 × 10(7) nucleated cell/mL) were cultured over glass reinforced hydroxyapatite pellets, at the same volume/mass ratio, for 30 days. Cultures performed in standard tissue culture plates were used as control. Results. The colonized BM concentrate/material constructs exhibited a representative osteoblastic proliferation/differentiation pathway, evidenced by a high alkaline phosphatase (ALP) activity, expression of collagen type 1, ALP, BMP-2, M-CSF, RANKL, and OPG, and formation of a calcium phosphate mineralized matrix. A clear improved behaviour was noticed compared to the BM aspirate/material constructs. Conclusions. The results suggest the benefit of using an autologous BM concentrate/material construct in the clinical setting, in bone regeneration applications.
authors: Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de São João, Largo Hernâni Monteiro, 4200 Porto, Portugal.
authors: J
authors: J
authors: Torres
authors: CEMUC, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors: A
authors: A
authors: Lopes
authors: CEMUC, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors: M A
authors: MA
authors: Lopes
authors: Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de São João, Largo Hernâni Monteiro, 4200 Porto, Portugal.
authors: M
authors: M
authors: Gutierres
authors: Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de São João, Largo Hernâni Monteiro, 4200 Porto, Portugal.
authors: A T
authors: AT
authors: Cabral
authors: Faculdade de Medicina Dentária da Universidade do Porto (FMDUP), Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.
authors: M H
authors: MH
authors: Fernandes
authors: Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de São João, Largo Hernâni Monteiro, 4200 Porto, Portugal.
authors: E
authors: E
authors: Monteiro
authors: University of Pittsburgh Medical Center (UPMC), Kaufman Building, Suite 1011, Pittsburgh, PA 15213, USA.
authors: C F
authors: CF
authors: van Eck
authors: CEMUC, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors: J D
authors: JD
authors: Santos
mesh_terms: D000368:Aged; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D001861:Bone Regeneration; D017886:Durapatite / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D005898:Glass / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D017886:Durapatite