title: Growth and alkaline phosphatase activity of Chattonella marina and Heterosigma akashiwo in response to phosphorus limitation.
abstract: The growth and alkaline phosphatase activity (APA) of two raphidophyceae species Chattonella marina and Heterosigma akashiwo were investigated in response to P-limitation and subsequent addition of dissolved inorganic phosphorus (DIP, NaH2PO4) and two dissolved organic phosphorus (DOP) compounds: guanosine 5-monophosphate (GMP) and triethyl phosphate (TEP). APA levels increased greatly after P-starvation as the decrease of the cellular phosphorus quotes (Qp). C. marina responded to P-limitation quickly and strongly, with 10-fold increase in APA within 24 hr after P-starvation. The larger difference between maximal and minimal QP values in C. marina indicated its high capacity in P storage. APA of H. akashiwo was maximally enlarged about 2.5 times at 48 hr of P-starvation. After the addition of nutrients, cell numbers of C. marina increased in all treatments including the P-free culture, demonstrating the higher endurance of C. marina to P-limitation. However, those of H. akashiwo increased only in DIP and GMP cultures. APA increased only after the addition of the monophosphate ester GMP. The results suggest that quick responses of C. marina to P-limitation, high capacity in P storage as well as endurance for P-depletion provide this species an ecological advantage in phytoplankton community competition under DIP-limited conditions.
authors: College of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China. Electronic address: twzh@jnu.edu.cn.
authors: Zhao-Hui
authors: ZH
authors: Wang
authors: College of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China.
authors: Yu
authors: Y
authors: Liang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D010758:Phosphorus / Q000172:deficiency / Q000378:metabolism*; D010839:Phytoplankton / Q000201:enzymology / Q000254:growth & development; D013045:Species Specificity; D058009:Stramenopiles / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D010758:Phosphorus; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; Dissolved inorganic phosphorus; Dissolved organic phosphorus; Growth; Phytoplankton