title: Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.
abstract: The expression of secretogranin III (SgIII) in chicken endocrine cells has not been investigated. There is limited data available for the immunohistochemical localization of SgIII in the brain, pituitary, and pancreatic islets of humans and rodents. In the present study, we used immunoblotting to reveal the similarities between the expression patterns of SgIII in the common endocrine glands of chickens and rats. The protein-protein interactions between SgIII and chromogranin A (CgA) mediate the sorting of CgA/prohormone core aggregates to the secretory granule membrane. We examined these interactions using co-immunoprecipitation in chicken endocrine tissues. Using immunohistochemistry, we also examined the expression of SgIII in a wide range of chicken endocrine glands and gastrointestinal endocrine cells (GECs). SgIII was expressed in the pituitary, pineal, adrenal (medullary parts), parathyroid, and ultimobranchial glands, but not in the thyroid gland. It was also expressed in GECs of the stomach (proventriculus and gizzard), small and large intestines, and pancreatic islet cells. These SgIII-expressing cells co-expressed serotonin, somatostatin, gastric inhibitory polypeptide, glucagon-like peptide-1, glucagon, or insulin. These results suggest that SgIII is expressed in the endocrine cells that secrete peptide hormones, which mature via the intragranular enzymatic processing of prohormones and physiologically active amines in chickens.
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Hiroshi
authors: H
authors: Gomi
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Satomi
authors: S
authors: Morikawa
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Naoki
authors: N
authors: Shinmura
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Hiroaki
authors: H
authors: Moki
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Tadashi
authors: T
authors: Yasui
authors: Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan (HG, SM, NS, HM, TY, AT)
authors: Azuma
authors: A
authors: Tsukise
authors: Laboratory of Secretion Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan (ST)
authors: Seiji
authors: S
authors: Torii
authors: Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical College, Asahikawa, Japan (TW)
authors: Tsuyoshi
authors: T
authors: Watanabe
authors: Laboratory of Molecular Life Sciences, Department of Biotechnology, Akita Prefectural University, Akita, Japan (YM, MH)
authors: Yoshinori
authors: Y
authors: Maeda
authors: Laboratory of Molecular Life Sciences, Department of Biotechnology, Akita Prefectural University, Akita, Japan (YM, MH)
authors: Masahiro
authors: M
authors: Hosaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002645:Chickens; D002864:Chromogranins / Q000378:metabolism*; D004702:Endocrine Glands / Q000378:metabolism*; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid
chemical_list: D002864:Chromogranins; C506167:secretogranin III
keywords: adrenal medulla; chicken endocrine system; chromogranin; gastrointestinal endocrine cells; pancreatic islets; prohormone; proteolytic processing; secretogranin; secretory granules; ultimobranchial glands