title: Citrinin detection using phage-displayed anti-idiotypic single-domain antibody for antigen mimicry.
abstract: Anti-idiotypic antibodies (AIds) can mimic antigen molecules and can thus offer an alternative to conventional antigens in immunoassays. In this study, citrinin (CIT) was chosen as a target analyte, and an anti-idiotypic single-domain antibody (VHH) was selected from a naïve alpaca VHHs library to serve as a surrogate for CIT hapten. The phage-displayed VHH was used as a signal-amplification carrier to develop an indirect competitive phage enzyme-linked immunosorbent assay (P-ELISA) for the sensitive detection of CIT. The half-inhibition concentration (IC50) of P-ELISA was 10.9 μg/kg, which was 9-fold better than that of conventional ELISA (IC50=102.1 μg/kg). Results on P-ELISA analysis of naturally contaminated samples were also consistent with those obtained by conventional ELISA. In conclusion, the proposed P-ELISA demonstrates the potential use of phage-displayed anti-idiotypic VHH as surrogate for small molecules and signal-amplification carrier to improve assay performance for more sensitive analyte detection in food safety monitoring.
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors: Yang
authors: Y
authors: Xu
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China; Department of Preventive Medicine, Gannan Medical College, Number 1 Yixueyuan Road, Ganzhou 341000, Jiangxi, China.
authors: Liang
authors: L
authors: Xiong
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China. Electronic address: liyanping@ncu.edu.cn.
authors: Yanping
authors: Y
authors: Li
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors: Yonghua
authors: Y
authors: Xiong
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors: Zhui
authors: Z
authors: Tu
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors: Jinheng
authors: J
authors: Fu
authors: State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University, Number 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors: Xiao
authors: X
authors: Tang
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D002953:Citrinin / Q000032:analysis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018716:Molecular Mimicry / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D002953:Citrinin
keywords: Anti-idiotypic antibody; Citrinin; Phage ELISA; Phage display; Single-domain antibodies