title: Metal-enhanced fluorescence and FRET on nanohole arrays excited at angled incidence.
abstract: The influence of experimental parameters on the performance of plasmonic sensors is of great importance in analytical sciences. The plasmon coupling conditions (angle of incidence, metal composition, laser frequency and excitation/emission properties of fluorophores) were thus investigated for surface plasmon-enhanced fluorescence on metallic nanohole arrays. Optimal fluorescence enhancements were achieved when the plasmon resonance, the excitation laser and the fluorophore's excitation wavelengths were matched. The enhancement of the acceptor emission of a rhodamine 6G(Rh6G)-Quasar670™ FRET pair was achieved on the nanohole arrays by tuning the plasmon wavelength with the maximal overlap of the donor's emission and acceptor excitation. Silver nanohole arrays achieved larger fluorescence enhancement than gold nanohole arrays at 532 nm, while gold nanohole arrays led to larger fluorescence enhancement at 635 nm. These results demonstrate the importance of tuning the plasmon coupling conditions for surface plasmon-enhanced fluorescence sensing.
authors: Departement de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC H3C 3J7, Canada. jf.masson@umontreal.ca.
authors: H-P
authors: HP
authors: Poirier-Richard
authors: M
authors: M
authors: Couture
authors: T
authors: T
authors: Brule
authors: J-F
authors: JF
authors: Masson
mesh_terms: D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D008670:Metals / Q000737:chemistry*; D049329:Nanostructures
chemical_list: D008670:Metals