title: Hepcidin levels in hyperprolactinemic women monitored by nanopore thin film based assay: correlation with pregnancy-associated hormone prolactin.
abstract: Hepcidin is a central regulator in human iron metabolism. Although it is often regarded as a promising indicator of iron status, the lack of effective quantification method has impeded the comprehensive assessment of its physiological and clinical significance. Herein we applied a newly established, nanopore film enrichment based hepcidin assay to examine the correlation between hepcidin and prolactin, the hormone with an important role during pregnancy and lactation. Women with pathologically elevated prolactin secretion (hyperprolactinemia) were found to have lower serum hepcidin compared to those with normal prolactin levels, without showing significant difference in other hepcidin-regulating factors. Moreover, prolactin-reducing drug bromocriptine mesylate resulted in elevated expression of the hepcidin in hyperprolactinemia patients. These findings suggest a possible role of prolactin in regulation of hepcidin, and may render hepcidin a useful biomarker for progress monitoring and treatment of iron-related diseases under hyperprolactinemic conditions.
  
  
  BACKGROUND
  The level of hepcidin has been shown to reflect the underlying iron status of the patient. Nonentheless, there is an urgent need of reliable, fast and easy-to-do hepcidin assay in the clinical setting. In this paper, the authors described a further modification of their previously described nanopore silica film-based enrichment approach for quantification of hepcidin and found correlation between hepcidin and prolactin. This new knowledge may add to current understanding of iron homeostasis during pregnancy.
authors: Chinese Academy of Sciences Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, China.
authors: Jing
authors: J
authors: Wang
authors: Chinese Academy of Sciences Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China.
authors: Gang
authors: G
authors: Liu
authors: Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
authors: Zi
authors: Z
authors: Xu
authors: Beijing Donghua Hospital, Beijing, China.
authors: Jiwei
authors: J
authors: Dai
authors: Beijing Donghua Hospital, Beijing, China.
authors: Ping
authors: P
authors: Song
authors: Chinese Academy of Sciences Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China.
authors: Jian
authors: J
authors: Shi
authors: Department of Nanomedicine, Houston Methodist Hospital Research Institute, Houston, TX, USA.
authors: Ye
authors: Y
authors: Hu
authors: College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, China.
authors: Zhongbo
authors: Z
authors: Hu
authors: Chinese Academy of Sciences Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China. Electronic address: niegj@nanoctr.cn.
authors: Guangjun
authors: G
authors: Nie
authors: Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China. Electronic address: chang7676@163.com.
authors: Yan-Zhong
authors: YZ
authors: Chang
authors: Chinese Academy of Sciences Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China. Electronic address: zhaoyl@nanoctr.cn.
authors: Yuliang
authors: Y
authors: Zhao
mesh_terms: D000328:Adult; D005260:Female; D064451:Hepcidins / Q000097:blood*; D006801:Humans; D006966:Hyperprolactinemia / Q000097:blood*; D008567:Membranes, Artificial; D058608:Nanopores; D011247:Pregnancy; D011388:Prolactin / Q000097:blood*
chemical_list: D064451:Hepcidins; D008567:Membranes, Artificial; D011388:Prolactin
keywords: Biomarker; Hepcidin; Hyperprolactinemia; MALDI-TOF MS; Nanopore silica film; Prolactin