title: rhCSF3 accelerates the proliferation of human melanocytes in culture through binding CSF3R and the expression of CSF3R transcripts.
abstract: Melanogenic paracrine and autocrine cytokine networks have recently been discovered in vitro between melanocytes and other types of skin cells. Granulocyte colony-stimulating factor receptor (CSF3R) controls the survival, proliferation and differentiation of many kinds of cells, including neutrophils. To understand the function of CSF3R and recombinant human granulocyte colony-stimulating factor (rhCSF3) on melanocyte proliferation, this study compared the expression of CSF3R and the effects of rhCSF3 in primary human melanocytes, neutrophils and HEL 92.1.7 cells. The results show that CSF3R is localized in the cytoplasm and on cell membranes of melanocytes and neutrophils. The percentage of CSF3R(+) melanocytes was higher than CSF3R(+) HEL 92.1.7 cells, but was lower than CSF3R(+) neutrophils. Both CSF3R mRNA and CSF3R protein levels in melanocytes were higher than in HEL 92.1.7 cells, but were lower than in neutrophils. Treatment with rhCSF3 increased the proliferation of human melanocytes, but not their tyrosinase activity. Transcripts of CSF3R in human melanocytes, M14, A375 melanoma and A431 squamous cell carcinoma cells were also detected. Expression of the CSF3R V3 transcript was lower in melanocytes than in M14, A375 melanoma and A431 squamous cell carcinoma cells. In conclusion, rhCSF3 can promote melanocyte proliferation through CSF3R without affecting tyrosinase activity.
authors: Dermatology Department, First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China, luyan6289@163.com.
authors: Yan
authors: Y
authors: Lu
authors: Ze
authors: Z
authors: Guo
authors: Mei-Hua
authors: MH
authors: Zhou
authors: Xue
authors: X
authors: Li
authors: Jie
authors: J
authors: Sun
authors: Qing-Li
authors: QL
authors: Gong
authors: Wen-Yuan
authors: WY
authors: Zhu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C517047:CSF3R protein, human; D003115:Colony-Stimulating Factors; D012333:RNA, Messenger; D016184:Receptors, Colony-Stimulating Factor; D011994:Recombinant Proteins; D014442:Monophenol Monooxygenase