title: Determination of synthetic food colorants in fish products by an HPLC-DAD method.
abstract: Reliable methods for quantification of synthetic water-soluble colors in complex food matrices are currently not available. The present work describes the development and validation of an improved protocol for the analysis of synthetic food colorants in complex food matrices presenting high protein and/or fat content. The method developed employs an extraction stage, followed by a subsequent sonification, centrifugation and concentration step. A final clean up via SPE on polyamide cartridges was also employed. The isolated colorants were separated and analyzed by an RP-HPLC/DAD system. High and consistent recoveries (min. 81%) and low RSDs (max. 6%) were achieved for all studied colorants. The issue of high fat content matrices was also addressed showing the need for an additional defatting step in the procedure. Overall, the protocol presented shows high precision and accuracy of detection and can provide the basis for future development of similar methods in other complex food matrices.
authors: General Chemical State Laboratory, Chemical Division of Piraeus and Aegean, 32 Etolikou Str., 18510 Piraeus, Greece. Electronic address: g.karanikolopoulos@2009.syzefxis.gov.gr.
authors: G
authors: G
authors: Karanikolopoulos
authors: General Chemical State Laboratory, Chemical Division of Piraeus and Aegean, 32 Etolikou Str., 18510 Piraeus, Greece.
authors: A
authors: A
authors: Gerakis
authors: General Chemical State Laboratory, Chemical Division of Piraeus and Aegean, 32 Etolikou Str., 18510 Piraeus, Greece.
authors: K
authors: K
authors: Papadopoulou
authors: General Chemical State Laboratory, Chemical Division of Piraeus and Aegean, 32 Etolikou Str., 18510 Piraeus, Greece. Electronic address: piraeus_aegean@gcsl.gr.
authors: I
authors: I
authors: Mastrantoni
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D005505:Food Coloring Agents / Q000032:analysis*
chemical_list: D005505:Food Coloring Agents
keywords: Diode-array detector; Fish products; Food analysis; Liquid chromatographic analysis; Synthetic dyes