title: Enhanced cellulase production by Penicillium oxalicum for bio-ethanol application.
abstract: Present study was focused on cellulase production from an indigenously isolated filamentous fungal strain, identified as Penicillium oxalicum. Initially, cellulase production under submerged fermentation in shake flasks resulted in cellulase activity of 0.7 FPU/mL. Optimization of process parameters enhanced cellulase production by 1.7-fold and resulted in maximum cellulase activity of 1.2 FPU/mL in 8 days. Cellulase production was successfully scaled-up to 7 L fermenter under controlled conditions and incubation time was reduced from 8 days to 4 days for achieving similar cellulase titer. Optimum pH and temperature for activity of the crude enzyme were pH 5 and 50 °C, respectively. At 50 °C the produced cellulase retained approximately 50% and 26% of its activity at 48 h and 72 h, respectively. Hydrolytic efficiency of P. oxalicum was comparable to commercial cellulase preparations which indicate its great potential for application in the lignocellulose hydrolysis.
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Reetu
authors: R
authors: Saini
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Jitendra Kumar
authors: JK
authors: Saini
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Mukund
authors: M
authors: Adsul
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Anil Kumar
authors: AK
authors: Patel
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Anshu
authors: A
authors: Mathur
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors: Deepak
authors: D
authors: Tuli
authors: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India. Electronic address: reetasinghania@gmail.com.
authors: Reeta Rani
authors: RR
authors: Singhania
mesh_terms: D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D008031:Lignin / Q000737:chemistry; D010407:Penicillium / Q000378:metabolism*; D010802:Phylogeny; D013696:Temperature
chemical_list: D003470:Culture Media; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D002480:Cellulase
keywords: Biomass; Bioreactor; Cellulase; Penicillium; Submerged fermentation