title: Simultaneous bile duct and portal vein ligation induces faster atrophy/hypertrophy complex than portal vein ligation: role of bile acids.
abstract: Portal vein ligation (PVL) induces atrophy/hypertrophy complex (AHC). We hypothesised that simultaneous bile duct and portal vein ligation (BPL) might induce proper bile acid (BA) retention to enhance AHC by activating BA-mediated FXR signalling in the intact liver and promoting apoptosis in the ligated liver. We established rat models of 90% BPL and 90% PVL and found that BPL was well-tolerated and significantly accelerated AHC. The enhanced BA retention in the intact liver promoted hepatocyte proliferation by promoting the activation of FXR signalling, while that in the ligated liver intensified caspase3-mediated apoptosis. Decreasing the BA pools in the rats that underwent BPL could compromise these effects, whereas increasing the bile acid pools of rats that underwent PVL could induce similar effects. Second-stage resection of posterior-caudate-lobe-spearing hepatectomy was performed 5 days after BPL (B-Hx), PVL (V-Hx) or sham (S-SHx), as well as whole-caudate-lobe-spearing hepatectomy 5 days after sham (S-Hx). The B-Hx group had the most favourable survival rate (93.3%, the S-SHx group 0%, the S-Hx group 26.7%, the V-Hx group 56.7%, P < 0.01) and the most sustained regeneration. We conclude that BPL is a safe and effective method, and the acceleration of AHC was bile acid-dependent.
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Weizheng
authors: W
authors: Ren
authors: Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University.
authors: Geng
authors: G
authors: Chen
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Xiaofeng
authors: X
authors: Wang
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Aiqun
authors: A
authors: Zhang
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Chonghui
authors: C
authors: Li
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Wenping
authors: W
authors: Lv
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Ke
authors: K
authors: Pan
authors: Department &Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors: Jia-Hong
authors: JH
authors: Dong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001284:Atrophy; D001647:Bile Acids and Salts / Q000378:metabolism*; D001652:Bile Ducts / Q000601:surgery*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006984:Hypertrophy; D008099:Liver / Q000473:pathology*; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D011169:Portal Vein / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013656:Taurocholic Acid / Q000494:pharmacology
chemical_list: D001647:Bile Acids and Salts; D053148:Caspase 3; D051860:Fragile X Messenger Ribonucleoprotein 1; D013656:Taurocholic Acid