title: PE-Swab Direct STR Amplification of Forensic Touch DNA Samples.
abstract: The PE-Swab direct STR amplification workflow was developed to process low-level "touch DNA" samples. In this workflow, a forensic sample is first collected on a 4-mm PE-Swab (a novel sample collection device); two 2-mm punches containing collected samples are then generated from the PE-Swab and directly amplified for STR typing. Compared to the conventional STR workflow, which involves DNA extraction, purification, and elution volume reduction, the PE-Swab direct STR amplification workflow does not require sample preparation and takes <60 sec before a touch sample is ready for STR amplification. Because there is no DNA loss due to sample preparation, the PE-Swab workflow is more sensitive than the conventional STR workflow. The average peak height per sample obtained by the PE-swab workflow is 3 times higher than that from the conventional workflow with both low-level single source and two-contributor mixture samples tested in this study.
authors: Thermo Fisher Scientific 180 Oyster Point Blvd., South San Francisco, CA, 94080.
authors: Jason Y
authors: JY
authors: Liu
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D017598:Efficiency, Organizational; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D014110:Touch
chemical_list: D004247:DNA
keywords: DNA extraction; DNA typing; PE-Swab; case work samples; direct amplification; forensic science; touch DNA