title: Ki-67/MIB-1 immunostaining in a cohort of human gliomas.
abstract: Histopathological malignancy grading of human gliomas is limited by subjective interpretation of the morphological criteria. Assessment of mitotic activity is a cornerstone of grading these tumours, but mitotic figures can be hard to identify in haematoxylin-eosin stained sections. Thus, determining proliferative activity by means of Ki-67/MIB-1 immunostaining has become a useful supplement. However, this method has drawbacks, so continuous testing and evaluation are required for optimization and standardization. The aim of this study was to analyse and evaluate the Ki-67/MIB-1 proliferative indices (PIs) in a series of gliomas. We found that Ki-67/MIB-1 PIs correlated well with histological malignancy grade in all glioma subtypes, but a considerable overlap of PIs was observed between the malignancy groups. Consequently, Ki-67/MIB-1 immunostaining alone is not sufficient to adequately determine the malignancy grade. Therefore, future work is necessary to clarify the role of this immunostaining in the histopathological diagnosis of human gliomas.
authors: Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway ; Department of Pathology and Medical Genetics, St. Olavs Hospital, Trondheim University Hospital Trondheim, Norway.
authors: Anne J
authors: AJ
authors: Skjulsvik
authors: Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway.
authors: Jørgen N
authors: JN
authors: Mørk
authors: Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway.
authors: Morten O
authors: MO
authors: Torp
authors: Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway ; Department of Pathology and Medical Genetics, St. Olavs Hospital, Trondheim University Hospital Trondheim, Norway.
authors: Sverre H
authors: SH
authors: Torp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000473:pathology*; D005260:Female; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading / Q000379:methods*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen
keywords: Astrocytoma; brain tumour; diagnosis; glioblastoma; immunohistochemistry; proliferation