title: Controlled release of stromal cell-derived factor-1α from silk fibroin-coated coils accelerates intra-aneurysmal organization and occlusion of neck remnant by recruiting endothelial progenitor cells.
abstract: This study is to test the efficacy of stromal cell-derived factor-1α (SDF-1α)-coated coils together with endothelial progenitor cells (EPCs) transplantation in occluding aneurysms. Bone marrow-derived EPC surface markers were analyzed using flow cytometry. The migratory function of EPCs in response to SDF-1α was evaluated using a modified Boyden chamber assay. Capillary-like tube formation was assessed using Matrigel gel. Coil morphologies before and after coating with SDF-1α were observed under a scanning electron microscope. The level of SDF-1α in supernatants was measured by ELISA. Sprague-Dawley rats were randomly allocated into five groups. Histological analysis was performed on days 14 and 28 after coil implantation. The bone marrow-EPCs could express CD133, CD34, and VEGFR-2 and form tubule-like structures in vitro. Migratory ability of EPCs in the presence of SDF-1α-coated coils was similar to that in the presence of 5 ng/ml SDF-1α gradient. Sustained release of SDF-1α was achieved using silk fibroin as a carrier. In SDF-1α-coated coils + EPCs transplantation group, a well-organized fibrous tissue bridging the orifice of aneurysms was shown on days 14 and 28. On day 28, tissue organization was greater in the SDF-1α-coated coils group than in the unmodified coils group. Immunofluorescence showed α-smooth muscle actin-positive cells in organized tissue in sacs. Combined treatment with SDF-1α-coated coils and EPCs transplantation is a safe and effective treatment for rat aneurysms. This may provide a new strategy for endovascular therapy following aneurysmal subarachnoid hemorrhage.
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Yuyuan
authors: Y
authors: Gao
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Qiujing
authors: Q
authors: Wang
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Xubo
authors: X
authors: Cui
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Yaqi
authors: Y
authors: Liu
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Tao
authors: T
authors: Zheng
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Chengwei
authors: C
authors: Chen
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Chengmei
authors: C
authors: Sun
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Shuyun
authors: S
authors: Huang
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Xin
authors: X
authors: Wang
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Yanchao
authors: Y
authors: Liu
authors: Department of Neurosurgery, Zhujiang Hospital, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University Guangzhou City, Guangdong Province, P.R. China.
authors: Xiaodan
authors: X
authors: Jiang
authors: Scholl of Chemistry and Chemical Engineering, Sun Yat-Sen University Guangzhou City, Guangdong Province, P.R. China.
authors: Chi
authors: C
authors: Zeng
authors: Scholl of Chemistry and Chemical Engineering, Sun Yat-Sen University Guangzhou City, Guangdong Province, P.R. China.
authors: Daping
authors: D
authors: Quan
mesh_terms: D000818:Animals; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic / Q000295:instrumentation* / Q000379:methods; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D057510:Endovascular Procedures / Q000295:instrumentation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005349:Fibroins; D005455:Fluorescent Antibody Technique; D002532:Intracranial Aneurysm / Q000601:surgery*; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D054377:Chemokine CXCL12; D005349:Fibroins
keywords: Stromal cell-derived factor-1α; cerebral aneurysm; endothelial progenitor cells; endothelialization; recanalization; silk fibroin