title: Immunomodulatory effects of the Agaricus blazei Murrill-based mushroom extract AndoSan in patients with multiple myeloma undergoing high dose chemotherapy and autologous stem cell transplantation: a randomized, double blinded clinical study.
abstract: Forty patients with multiple myeloma scheduled to undergo high dose chemotherapy with autologous stem cell support were randomized in a double blinded fashion to receive adjuvant treatment with the mushroom extract AndoSan, containing 82% of Agaricus blazei Murrill (19 patients) or placebo (21 patients). Intake of the study product started on the day of stem cell mobilizing chemotherapy and continued until the end of aplasia after high dose chemotherapy, a period of about seven weeks. Thirty-three patients were evaluable for all study endpoints, while all 40 included patients were evaluable for survival endpoints. In the leukapheresis product harvested after stem cell mobilisation, increased percentages of Treg cells and plasmacytoid dendritic cells were found in patients receiving AndoSan. Also, in this group, a significant increase of serum levels of IL-1ra, IL-5, and IL-7 at the end of treatment was found. Whole genome microarray showed increased expression of immunoglobulin genes, Killer Immunoglobulin Receptor (KIR) genes, and HLA genes in the Agaricus group. Furthermore, AndoSan displayed a concentration dependent antiproliferative effect on mouse myeloma cells in vitro. There were no statistically significant differences in treatment response, overall survival, and time to new treatment. The study was registered with Clinicaltrials.gov NCT00970021.
authors: Deparment of Haematology, Oslo University Hospital, 0424 Oslo, Norway ; Institute of Clinical Medicine, University of Oslo, 0372 Oslo, Norway.
authors: Jon-Magnus
authors: 0000-0003-4290-7746
authors: JM
authors: Tangen
authors: Department of Pathology, Oslo University Hospital, 0424 Oslo, Norway ; Laboratory Medicine Program, Toronto General Hospital, University Health Network, Toronto, ON, Canada M5G 2C4.
authors: Anne
authors: A
authors: Tierens
authors: Laboratory of Hematology, GIGA-Research, University of Liege, 4000 Sart Tilman, Belgium.
authors: Jo
authors: J
authors: Caers
authors: Laboratory of Hematology, GIGA-Research, University of Liege, 4000 Sart Tilman, Belgium.
authors: Marilene
authors: M
authors: Binsfeld
authors: Department Medical Biochemistry, Oslo University Hospital, 0424 Oslo, Norway.
authors: Ole Kristoffer
authors: OK
authors: Olstad
authors: Department Medical Biochemistry, Oslo University Hospital, 0424 Oslo, Norway.
authors: Anne-Marie Siebke
authors: AM
authors: Trøseid
authors: Department of Pathology, Oslo University Hospital, 0424 Oslo, Norway.
authors: Junbai
authors: J
authors: Wang
authors: Deparment of Haematology, Oslo University Hospital, 0424 Oslo, Norway ; Institute of Clinical Medicine, University of Oslo, 0372 Oslo, Norway.
authors: Geir Erland
authors: GE
authors: Tjønnfjord
authors: Institute of Clinical Medicine, University of Oslo, 0372 Oslo, Norway ; Department of Immunology and Transfusion Medicine, Oslo University Hospital, 0424 Oslo, Norway.
authors: Geir
authors: G
authors: Hetland
mesh_terms: D000328:Adult; D000364:Agaricus / Q000737:chemistry*; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D045424:Complex Mixtures; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D005786:Gene Expression Regulation; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology / Q000627:therapeutic use*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007937:Leukapheresis; D008297:Male; D051379:Mice; D008875:Middle Aged; D009101:Multiple Myeloma / Q000188:drug therapy* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D016019:Survival Analysis; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: C579023:AndoSan; D014408:Biomarkers, Tumor; D018925:Chemokines; D045424:Complex Mixtures; D007155:Immunologic Factors; D036341:Intercellular Signaling Peptides and Proteins; D014020:Tissue Extracts