title: High resolution Raman spectroscopy mapping of stem cell micropatterns.
abstract: We report on the use of high resolution Raman spectroscopy mapping combined with a micro-engineered stem cell platform. This technique obtains quantitative information about the concentration of individual intracellular molecules such as proteins, lipids, and other metabolites, while tightly controlling cell shape and adhesion. This new quantitative analysis will prove highly relevant for in vitro drug screening applications and regenerative medicine.
authors: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk.
authors: Thomas C
authors: TC
authors: von Erlach
authors: Martin A B
authors: MA
authors: Hedegaard
authors: Molly M
authors: MM
authors: Stevens
mesh_terms: D002460:Cell Line; D048430:Cell Shape; D006801:Humans; D008055:Lipids / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology*; D011506:Proteins / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids; D011506:Proteins