title: Development of an antigen capture enzyme-linked immunosorbent assay for virus detection based on porcine epidemic diarrhea virus monoclonal antibodies.
abstract: Porcine epidemic diarrhea virus (PEDV), a coronavirus, can cause acute diarrhea and dehydration in pigs. In the current study, two positive monoclonal cell lines (5D7 and 3H4) specific for PEDV were established, and the immunoreactivity of the monoclonal antibodies was confirmed by immunofluorescence and dot-immunobinding assays. A method, termed antigen capture enzyme-linked immunosorbent assay (AC-ELISA), which used the monoclonal antibody 5D7 as the detecting antibody and rabbit antiserum of PEDV protein S as the capture antibody, was developed. Compared with the reverse transcription polymerase chain reaction method of detecting PEDV in fecal samples, AC-ELISA showed similar sensitivity and specificity. These results suggested that AC-ELISA would be useful for the diagnosis and epidemiological studies of PEDV.
authors: Veterinary Microbiology Department, College of Veterinary Medicine, Northeast Agricultural University , Harbin, People's Republic of China .
authors: Zanyu
authors: Z
authors: Wang
authors: Yin
authors: Y
authors: Jiyuan
authors: Chen
authors: C
authors: Su
authors: Qiao
authors: Q
authors: Xinyuan
authors: Tang
authors: T
authors: Lijie
authors: Li
authors: L
authors: Yijing
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D003955:Diagnostic Tests, Routine / Q000379:methods*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology*; D051379:Mice; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral