title: Effects of adipocyte-secreted factors on decidualized endometrial cells: modulation of endometrial receptivity in vitro.
abstract: Obesity is defined as an excessive accumulation of adipose tissue that may lead to health complications. Mounting evidence indicates that obesity has a negative impact on fertility. Yet, the link between adipose tissue biology and infertility remains unclear. We aimed to investigate the communication between the adipose tissue and the reproductive system and the importance of this cross talk for the development of a receptive endometrium. To that end, we generated an in vitro model with endometrial and adipocyte cell lines. Sexual hormones, progesterone and estradiol, were used to decidualize endometrial cells and sensitize adipocytes. Decidualization produced a simultaneous increase of adipokine receptors in endometrial cells paralleling changes in their receptivity status. Furthermore, sensitization of 3T3-L1 adipocytes increased mRNA levels of leptin and resistin and decreased the expression of adiponectin and chemerin levels. This was accompanied by increased isoproterenol-induced lipolysis and reduced insulin-stimulated glucose uptake. Lastly, conditioned culture medium of those sensitized adipocytes was used to feed endometrial cells. This treatment resulted in (i) upregulation of genes previously identified as positive regulators of endometrial receptivity, such as leukemia inhibitory factor and glutathione peroxidase 3, and (ii) downregulation of interleukin-15 and mucin1, both genes negatively related with endometrial receptivity. Our results indicate that the endocrine communication between adipose tissue and the reproductive system is bidirectional and stress the importance of the adipose tissue to modulate the reproductive fitness.
authors: Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Hospital Universitario Miguel Servet, Zaragoza, Spain.
authors: Silvia
authors: S
authors: Gamundi-Segura
authors: Jose
authors: J
authors: Serna
authors: Sergio
authors: S
authors: Oehninger
authors: Jose A
authors: JA
authors: Horcajadas
authors: Jose M
authors: JM
authors: Arbones-Mainar
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000328:Adult; D000818:Animals; D017077:Culture Media, Conditioned; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005298:Fertility; D015870:Gene Expression; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000378:metabolism*; D051379:Mice; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D019899:Paracrine Communication; D054417:Receptors, Adipokine / Q000378:metabolism; D055815:Young Adult
chemical_list: D017077:Culture Media, Conditioned; D054417:Receptors, Adipokine