title: Pyridine-biquinoline-metal complexes for sensing pyrophosphate and hydrogen sulfide in aqueous buffer and in cells.
abstract: Herein, we report a new pyridine-biquinoline-derivative fluorophore L for effectively sensing pyrophosphate (PPi) and monohydrogen sulfide (HS(-)) in aqueous buffer and in living cells. L could selectively coordinate with metal ions (M(n+)) in Groups IB and IIB to form L-M(n+) complexes with 1:1 stoichiometry, resulting in fluorescence quenching via photoinduced electron transfer (PET) mechanism. L-Zn(2+) complex was applied to competitively coordinate with PPi to form a new "ate"-type complex, turning on the fluorescence by a 21-fold-increase. The limit of detection (LOD) of this assay for PPi detection in aqueous buffer is 0.85 μM. L-Cu(2+) complex was applied for highly selective detection of HS(-) with an excellent sensitivity by 25-fold decomplexation-induced fluorescence increase. LOD of L-Cu(2+) complex for HS(-) detection in aqueous buffer is 2.24 μM. With the in vitro data obtained, we successfully applied these two complexes for sequential imaging Zn(2+) and PPi, Cu(2+) and HS(-) in living cells, respectively. Since PPi and HS(-) occur in vascular calcification in positive correlation, our multifunctional probe L might help doctors to more precisely diagnose this disease in vivo in the future. For example, we could use radioactive tracer L-(64)Cu for qualitative and quantitative positron emission tomography/computed tomography (PET/CT) imaging of HS(-) in vivo.
authors: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , Hefei, Anhui 230026, China.
authors: Zijuan
authors: Z
authors: Hai
authors: Yajie
authors: Y
authors: Bao
authors: Qingqing
authors: Q
authors: Miao
authors: Xiaoyi
authors: X
authors: Yi
authors: Gaolin
authors: G
authors: Liang
mesh_terms: D002021:Buffers; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D011756:Diphosphates / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D057230:Limit of Detection; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D002021:Buffers; D056831:Coordination Complexes; D011756:Diphosphates; D005456:Fluorescent Dyes; D011725:Pyridines; D011804:Quinolines; D014867:Water; C107241:diphosphoric acid; D003300:Copper; D015032:Zinc; C023666:pyridine; D006862:Hydrogen Sulfide