title: A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells.
abstract: HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.
authors: Department of Biology and Chemistry, City University of Hong Kong , 83 Tat Chee Avenue, Kowloon, Hong Kong, People's Republic of China.
authors: Huatang
authors: H
authors: Zhang
authors: Ruochuan
authors: R
authors: Liu
authors: Yi
authors: Y
authors: Tan
authors: William Haowei
authors: WH
authors: Xie
authors: Haipeng
authors: H
authors: Lei
authors: Hon-Yeung
authors: HY
authors: Cheung
authors: Hongyan
authors: H
authors: Sun
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009589:Nitrogen Oxides / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; C039900:nitroxyl
keywords: FRET probe; HNO; bioimaging; fluorescent probe; ratiometric