title: Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin.
abstract: Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of its effects on coagulation. This work shows for the first time the cloning and sequencing of antithrombin from a snake species. This predicted protein is composed by 430 amino acids and presents about 64.5% sequence identity to human antithrombin. Biacore experiments revealed that the binding affinity of Bothrops jararaca snake antithrombin to heparin was ~30 times higher than that of human antithrombin. Furthermore, Bothrops jararaca antithrombin is more effective in preventing acute inflammation induced by carrageenan when compared to human antithrombin. Hence, the results showed herein suggest that Bothrops jararaca antithrombin can play a key role in the control of acute inflammation and that this molecule might be used as a pharmacological tool and as a prototype for drug development.
authors: Karen de
authors: Kd
authors: Morais-Zani
authors: Kathleen F
authors: KF
authors: Grego
authors: Ricardo J S
authors: RJ
authors: Torquato
authors: Caroline S
authors: CS
authors: Silva
authors: Aparecida S
authors: AS
authors: Tanaka
authors: Instituto Butantan, Laboratorio de Herpetologia, Avenida Vital Brazil, 1500, 05503-900, Sao Paulo, Brasil. anita.azevedo@butantan.gov.br.
authors: Anita M
authors: AM
authors: Tanaka-Azevedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D058833:Antithrombin Proteins / Q000737:chemistry / Q000235:genetics / Q000627:therapeutic use*; D017837:Bothrops / Q000235:genetics*; D002351:Carrageenan; D003001:Cloning, Molecular; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics / Q000627:therapeutic use*; D016415:Sequence Alignment
chemical_list: D000893:Anti-Inflammatory Agents; D058833:Antithrombin Proteins; D030162:Reptilian Proteins; D002351:Carrageenan