title: Water-soluble polysaccharide isolated with alkali from the stem of Physalis alkekengi L.: structural characterization and immunologic enhancement in DNA vaccine.
abstract: A water-soluble polysaccharide (WSPA) was isolated with alkali and purified from the mature stem of Physalis alkekengi L. WSPA (Mw=31kDa) was an acid heteropolysaccharide, which consisted of Rha, Ara, Xyl, Gal, Glc and GalA in ratio of 1.0:2.5:0.8:2.7:4.4:1.4. The results from structural analysis indicated backbone and branches of WSPA were composed of (1→3)-linked Glc, (1→3)-linked Gal, (1→2)-linked Xyl, (1→2)-linked Ara, and (1→2)-linked Rha residues. However, GalA was distributed only in the backbone of WSPA. All branches of WSPA were at O-2 of (1→6)-linked Gal and terminated with Glc. More importantly, it was found that WSPA significantly enhanced specific antibody IgG response with higher titers of IgG1 as well as IgG2b (p<0.05) in mice immunized with DNA vaccine. Therefore, WSPA can be considered as a potential adjuvant candidate in DNA vaccine.
authors: School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
authors: Jingjing
authors: J
authors: Yang
authors: School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
authors: Fan
authors: F
authors: Yang
authors: School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
authors: Huimin
authors: H
authors: Yang
authors: School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China. Electronic address: wanggy737@nenu.edu.cn.
authors: Guiyun
authors: G
authors: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D000468:Alkalies / Q000737:chemistry; D000818:Animals; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000468:Alkalies; D018841:HSP90 Heat-Shock Proteins; D007136:Immunoglobulins; D010936:Plant Extracts; D011134:Polysaccharides; D019444:Vaccines, DNA
keywords: Adjuvant; DNA vaccine; Physalis alkekengi L.; Polysaccharide; Structure analysis