title: Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor.
abstract: Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.
authors: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors: Luna
authors: L
authors: Izuhara
authors: Department of Anatomy, The Jikei University School of Medicine, Tokyo, Japan.
authors: Norifumi
authors: N
authors: Tatsumi
authors: Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Shuji
authors: S
authors: Miyagawa
authors: Laboratory of Small Animal Surgery I, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
authors: Satomi
authors: S
authors: Iwai
authors: Meiji University International Institute for Bio-Resource Research, Kawasaki, Japan.
authors: Masahito
authors: M
authors: Watanabe
authors: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors: Shuichiro
authors: S
authors: Yamanaka
authors: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors: Yuichi
authors: Y
authors: Katsuoka
authors: Meiji University International Institute for Bio-Resource Research, Kawasaki, Japan.
authors: Hiroshi
authors: H
authors: Nagashima
authors: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors: Hirotaka J
authors: HJ
authors: Okano
authors: Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors: Takashi
authors: T
authors: Yokoo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018958:CD55 Antigens / Q000737:chemistry / Q000235:genetics*; D002371:Cat Diseases / Q000601:surgery; D002415:Cats; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D003001:Cloning, Molecular; D042783:Endothelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006084:Graft Rejection; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D051436:Renal Insufficiency, Chronic / Q000662:veterinary; D013552:Swine; D014162:Transfection; D014183:Transplantation, Heterologous / Q000009:adverse effects
chemical_list: D018958:CD55 Antigens; D007770:L-Lactate Dehydrogenase