title: Critical methodological factors in diagnosing minimal residual disease in hematological malignancies using quantitative PCR.
abstract: Hematological malignancies are a heterogeneous group of cancers with respect to both presentation and prognosis, and many subtypes are nowadays associated with aberrations that make up excellent molecular targets for the quantification of minimal residual disease. The quantitative PCR methodology is outstanding in terms of sensitivity, specificity and reproducibility and thus an excellent choice for minimal residual disease assessment. However, the methodology still has pitfalls that should be carefully considered when the technique is integrated in a clinical setting.
authors: Department of Haematology, HemoDiagnostic Laboratory, Aarhus University Hospital, Tage-Hansens Gade 2, Aarhus 8000, Denmark.
authors: Charlotte Guldborg
authors: CG
authors: Nyvold
mesh_terms: D019337:Hematologic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D018365:Neoplasm, Residual / Q000175:diagnosis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: AML; CML; MCL; hematology; minimal residual disease; qPCR