title: Cytotoxic effect of extract from Dunaliella salina against SH-SY5Y neuroblastoma cells.
abstract: Cytotoxic effects of essential oils extracted from Dunaliella salina on SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determination of the composition of essential oils found in Dunaliella salina extract. All experimented concentrations of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than the tested concentrations of the extract on ECV304 human endothelial cells used as a control. Fifthy compounds were detected in GC-MS analysis of the extract, and five major compounds were predominantly found as follows: octadecanoic acid, methyl ester (27.43%); hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39%); octadecanoic acid (5.03%), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells might be due to high concentrations of octadecanoic acid and hexadecanoic acid. Furthermore, results indicate that the extract demonstrates some proliferative effect on ECV304 cells in a dose-dependent manner between 0.25 and 5 μg/ml. These results suggest that Dunaliella salina may have anticancer potential against human neuroblastoma cells.
authors: Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey. belkisatasever.arslan@uskudar.edu.tr.
authors: Belkis
authors: B
authors: Atasever-Arslan
authors: Kaan
authors: K
authors: Yilancioglu
authors: Maide G
authors: MG
authors: Bekaroglu
authors: Emre
authors: E
authors: Taskin
authors: Eyup
authors: E
authors: Altinoz
authors: Selim
authors: S
authors: Cetiner
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology*; D009822:Oils, Volatile / Q000008:administration & dosage*; D010936:Plant Extracts / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts