title: Pyrroloquinoline Quinone Slows Down the Progression of Osteoarthritis by Inhibiting Nitric Oxide Production and Metalloproteinase Synthesis.
abstract: Osteoarthritis (OA) is the most common arthritis and also one of the major causes of joint pain in elderly people. The aim of this study was to investigate the effects of pyrroloquinoline quinone (PQQ) on degenerated-related changes in osteoarthritis (OA). SW1353 cells were stimulated with IL-1β to establish the chondrocyte injury model in vitro. PQQ was administrated into SW1353 cultures 1 h before IL-1β treatment. Amounts of MMP-1, MMP-13, P65, IκBα, ERK, p-ERK, P38, and p-P38 were measured via western blot. The production of NO was determined by Griess reaction assay and reflected by the iNOS level. Meniscal-ligamentous injury (MLI) was performed on 8-week-old rats to establish the OA rat model. PQQ was injected intraperitoneally 3 days before MLI and consecutively until harvest, and the arthritis cartilage degeneration level was assessed. The expressions of MMP-1 and MMP-13 were significantly downregulated after PQQ treatment compared with that in IL-1β alone group. NO production and iNOS expression were decreased by PQQ treatment compared with control group. Amounts of nucleus P65 were upregulated in SW1353 after stimulated with IL-1β, while PQQ significantly inhibited the translocation. In rat OA model, treatment with PQQ markedly decelerated the degeneration of articular cartilage. These findings suggested that PQQ could inhibit OA-related catabolic proteins MMPs expression, NO production, and thus, slow down the articular cartilage degeneration and OA progression. Owing to its beneficial effects, PQQ is expected to be a novel pharmacological application in OA clinical prevention and treatment in the near future.
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Ran
authors: R
authors: Tao
authors: Shitao
authors: S
authors: Wang
authors: Xiaopeng
authors: X
authors: Xia
authors: Youhua
authors: Y
authors: Wang
authors: Yi
authors: Y
authors: Cao
authors: Yuejiao
authors: Y
authors: Huang
authors: Xinbao
authors: X
authors: Xu
authors: Zhongbing
authors: Z
authors: Liu
authors: Peichao
authors: P
authors: Liu
authors: Xiaohang
authors: X
authors: Tang
authors: Chun
authors: C
authors: Liu
authors: Gan
authors: G
authors: Shen
authors: Dongmei
authors: D
authors: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010003:Osteoarthritis / Q000188:drug therapy / Q000378:metabolism*; D045542:PQQ Cofactor / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats
chemical_list: D009569:Nitric Oxide; D045542:PQQ Cofactor; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat; C576840:MMP1 protein, rat; D020781:Matrix Metalloproteinase 1