title: Separation of chemical constituents from three plant medicines by counter-current chromatography using a three-phase solvent system at a novel ratio.
abstract: A solvent system of n-hexane, methyl acetate, acetonitrile, and water at a novel volume ratio of 4:3:4:4 forms three layers, i.e. upper phase (UP), middle phase (MP), and lower phase (LP), with a volume ratio of 1:1.20:1.42 at room temperature (25°C). All three two-phases from this three-phase solvent system were successfully used to separate some chemical constituents from three plant medicines with counter-current chromatography (CCC). Eight coumarins (B1-B8) were obtained from petroleum ether extract of fresh roots of Angelica dahurica (Baizhi) with a stationary phase of UP and a mobile phase of LP. Six diarylheptanoids (L1-L6) were obtained from petroleum ether extract of dried rhizomes of Alpinia officinarum (Liangjiang) with a stationary phase of UP and a mobile phase of MP. Three chemical constituents (Z1-Z3) were obtained from ethyl acetate extract of fresh rhizomes of Anemarrhena asphodeloides (Zhimu) with a stationary phase of MP and a mobile phase of LP. Preparative HPLC was used for further purification if necessary. Seventeen chemical constituents were identified as oxypeucedanin hydrate (B1), byakangelicin (B2), byakangelicol (B3), bergapten (B4), oxypeucedanin (B5), imperatorin (B6), phellopterin (B7), isoimperatorin (B8), 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (L1), 7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-4E-en-3-heptanone (L2), 5-hydroxy-1,7-diphenyl-3-heptanone (L3), 1,7-diphenyl-4E-en-3-heptanone (L4), 5-hydroxy-1,7-diphenyl-4E,6E-dien-3-heptanone (L5), isomers of 1,7-diphenyl-3,5-heptandione and 5-hydroxy-1,7-diphenyl-4E-en-3-heptanone (L6), mangiferin (Z1), timosaponin A-III (Z2), and 2,6,4'-trihydroxy-4-methoxy-benzophenone (Z3) by means of MS, (1)H and (13)C NMR studies. Five compounds of B3, L3, L5, L6, and Z3 were isolated by CCC for the first time.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Xiaoyi
authors: X
authors: Wu
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: chaozhimao@163.com.
authors: Zhimao
authors: Z
authors: Chao
authors: Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Chun
authors: C
authors: Wang
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Li
authors: L
authors: Yu
mesh_terms: D032429:Alpinia / Q000737:chemistry; D031403:Anemarrhena; D029969:Angelica / Q000737:chemistry; D003377:Countercurrent Distribution; D059625:Liquid-Liquid Extraction / Q000379:methods*; D010936:Plant Extracts / Q000302:isolation & purification*; D027343:Rhizome / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012997:Solvents
keywords: Alpinia officinarum; Anemarrhena asphodeloides; Angelica dahurica; Chemical constituent; Counter-current chromatography (CCC); Three-phase solvent system