title: Pulse Field Gel Electrophoresis.
abstract: Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.
authors: Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA.
authors: Batu K
authors: BK
authors: Sharma-Kuinkel
authors: Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA.
authors: Thomas H
authors: TH
authors: Rude
authors: Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA. fowle003@mc.duke.edu.
authors: Vance G
authors: VG
authors: Fowler
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype
chemical_list: D004269:DNA, Bacterial; D004247:DNA; D004262:DNA Restriction Enzymes
keywords: Genomic DNA; Genotyping technique; Pulse field gel electrophoresis; Restriction enzyme