title: Probing the role of scaffold dimensionality and media composition on matrix production and phenotype of fibroblasts.
abstract: Porous sponges, hydrogels, and micro/nanofibrous matrix are most commonly used three dimensional (3D) biomaterials in tissue engineering; however, reciprocal interaction between internal dimensionality of biomaterials and fibroblasts remains largely unexplored. Such studies would have potential to generate valuable insights about wound healing, tissue morphogenesis and homeostasis. To the best of our knowledge this is the first study to evaluate functionality of porous collagen matrix and collagen gels for in vitro culture of fibroblasts while investigating the role of culture media composition in modulating morphology, phenotype, extracellular matrix (ECM)-related gene expression and protein synthesis by fibroblasts. Encapsulation of fibroblasts in collagen gel was found to be more effective for ECM production compared to scaffold-based culture, as evidenced by enhanced collagen type I, elastin, lysyl oxidase, aggrecan gene expression. High glucose media induced spindle like morphology of typical in vivo fibroblasts and enhanced collagen production compared to other media. This variation in biosynthesis in different glucose concentrations was possibly due to endogenous activation of TGF-β or by an increase in ATP consuming anabolic pathways in high glucose concentration.
authors: Department of Textile Technology, IIT Delhi, New Delhi 110016, India; Centre for Biomedical Engineering, IIT Delhi, New Delhi, 110016, India.
authors: S
authors: S
authors: Chawla
authors: Department of Textile Technology, IIT Delhi, New Delhi 110016, India.
authors: S
authors: S
authors: Chameettachal
authors: Department of Textile Technology, IIT Delhi, New Delhi 110016, India. Electronic address: sghosh08@textile.iitd.ac.in.
authors: S
authors: S
authors: Ghosh
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D053674:Aggrecans / Q000378:metabolism; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology*; D004549:Elastin / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D010641:Phenotype; D014176:Protein Biosynthesis / Q000187:drug effects; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D053674:Aggrecans; D024042:Collagen Type I; D003470:Culture Media; D020100:Hydrogels; D016212:Transforming Growth Factor beta; D000255:Adenosine Triphosphate; D004549:Elastin; D008249:Protein-Lysine 6-Oxidase; D005947:Glucose
keywords: Collagen gel; Fibroblast; Porous scaffold