title: Novel colorimetric immunoassay for ultrasensitive monitoring of brevetoxin B based on enzyme-controlled chemical conversion of sulfite to sulfate.
abstract: A simple colorimetric immunoassay for quantitative monitoring of brevetoxin B on a functionalized magnetic bead by using glucose oxidase (GOx)/antibrevetoxin antibody-labeled gold nanoparticle as the signal transduction tag was developed. The assay was carried out on the basis of GOx-controlled sulfite-to-sulfate chemical conversion with a silver(I)-3,3',5,5'-tetramethylbenzidine [Ag(I)-TMB] system. Initially, the sulfite was used as an inhibitor of Ag(I) to hinder the color development of TMB due to the formation of insoluble silver sulfite. Accompanying H2O2 generation with GOx-catalyzed glucose, the sulfite was converted into the sulfate, thus resulting in the colorless-to-blue change. Under the optimal conditions, the absorbance decreased with increasing brevetoxin B from 0.5 to 200 ng/kg with a detection limit of 0.1 ng/kg (ppt). The precision and specificity were acceptable. Furthermore, the methodology gave results matching well with the referenced brevetoxin ELISA kit for monitoring of spiked Musculista senhousia samples.
authors: State Key Laboratory of Photocatalysis on Energy and Environment, Key Laboratory of Analysis and Detection for Food Safety (Fujian Province and Ministry of Education), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University , Fuzhou 350108, People's Republic of China.
authors: Wenqiang
authors: W
authors: Lai
authors: Junyang
authors: J
authors: Zhuang
authors: Dianping
authors: D
authors: Tang
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D005949:Glucose Oxidase / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008387:Marine Toxins / Q000032:analysis*; D035964:Oxocins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*; D013431:Sulfates / Q000737:chemistry*; D013447:Sulfites / Q000737:chemistry*; D000095702:Polyether Toxins
chemical_list: D005949:Glucose Oxidase; D008387:Marine Toxins; D035964:Oxocins; D013431:Sulfates; D013447:Sulfites; C043466:brevetoxin B; D000095702:Polyether Toxins
keywords: brevetoxin B; colorimetric immunoassay; marine biotoxin; sulfite-to-sulfate enzyme-controlled chemical conversion