title: Phenotype and function of nasal dendritic cells.
abstract: Intranasal (i.n.) vaccination generates immunity across local, regional, and distant sites. However, nasal dendritic cells (DCs), pivotal for the induction of i.n. vaccine-induced immune responses, have not been studied in detail. Here, by using a variety of parameters, we define nasal DCs in mice and humans. Distinct subsets of "classical" DCs, dependent on the transcription factor zbtb46 were identified in the murine nose. The murine nasal DCs were Fms-related tyrosine 3 kinase ligand responsive and displayed unique phenotypic and functional characteristics, including the ability to present antigen, induce an allogeneic T-cell response, and migrate in response to lipopolysaccharide or live bacterial pathogens. Importantly, in a cohort of human volunteers, BDCA-1(+) DCs were observed to be the dominant nasal DC population at steady state. During chronic inflammation, the frequency of both BDCA-1(+) and BDCA-3(hi) DCs was reduced in the nasal tissue, associating the loss of these immune sentinels with chronic nasal inflammation. The present study is the first detailed description of the phenotypic, ontogenetic, and functional properties of nasal DCs, and will inform the design of preventative immunization strategies as well as therapeutic modalities against chronic rhinosinusitis.
authors: 1] Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: H
authors: H
authors: Lee
authors: 1] Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: D
authors: D
authors: Ruane
authors: 1] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: K
authors: K
authors: Law
authors: Department of Otorhinolaryngology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Y
authors: Y
authors: Ho
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: A
authors: A
authors: Garg
authors: 1] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Flow Cytometry Shared Resource Facility, Icahn School of Medicine, New York, New York, USA.
authors: A
authors: A
authors: Rahman
authors: Laboratory of Mucosal Immunology, Rockefeller University, New York, New York, USA.
authors: D
authors: D
authors: Esterházy
authors: Laboratory of Cellular Immunology and Physiology, Institut de Recherches Cliniques de Montréal and Department of Microbiology and Immunology, Université de Montréal, Montreal, Quebec, Canada.
authors: C
authors: C
authors: Cheong
authors: Department of Otorhinolaryngology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: E
authors: E
authors: Goljo
authors: Bobby R. Alford Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA.
authors: A G
authors: AG
authors: Sikora
authors: Laboratory of Mucosal Immunology, Rockefeller University, New York, New York, USA.
authors: D
authors: D
authors: Mucida
authors: 1] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: B K
authors: BK
authors: Chen
authors: Department of Otorhinolaryngology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: S
authors: S
authors: Govindraj
authors: Laboratory of Molecular Immunology, Rockefeller University, New York, New York, USA.
authors: G
authors: G
authors: Breton
authors: 1] Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA [2] Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: S
authors: S
authors: Mehandru
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000166:cytology* / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology / Q000473:pathology; D012852:Sinusitis / Q000276:immunology / Q000473:pathology; D018180:Thrombomodulin; D014157:Transcription Factors / Q000276:immunology
chemical_list: D018949:Antigens, CD1; D000954:Antigens, Surface; C437944:CD1C protein, human; D004268:DNA-Binding Proteins; D006023:Glycoproteins; C546931:THBD protein, human; D018180:Thrombomodulin; D014157:Transcription Factors; C485514:ZBTB7A protein, human; C574226:Zbtb46 protein, mouse