title: Developing an ultra non-fouling SU-8 and PDMS hybrid microfluidic device by poly(amidoamine) engraftment.
abstract: A new strategy to prepare an ultra non-fouling SU-8/poly(dimethylsiloxane) (PDMS) hybrid microfluidic system is presented. We report a simple method to bond both SU-8 and PDMS surfaces to prepare the hybrid microfluidic device and then simultaneously modify both SU-8 and PDMS surfaces using poly(amidoamine) (PAMAM), a highly hydrophilic dendrimer to enhance non-fouling properties of the hybrid microfluidic channel. Water contact angle, atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) are used to characterize and confirm surface modifications. Finally the non-fouling performance of the resulting microfluidic system is tested using both microbeads and Escherichia coli O157:H7 bacterial cells. We demonstrate that the obtained hybrid microfluidic system shows a significant microbead adsorption suppression of 99.7%, an ultra non-fouling performance that has not been reported in the literature before; in addition, 95% bacteria surface adsorption suppression is also obtained. Considering the significantly improved non-fouling performance and the ease of preparing the hybrid microfluidic system, we anticipate that this strategy will find wide applications in biosensing and detection.
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Yubo
authors: Y
authors: Qin
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Poying
authors: P
authors: Yeh
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Xingkai
authors: X
authors: Hao
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada. Electronic address: xcao@eng.uottawa.ca.
authors: Xudong
authors: X
authors: Cao
mesh_terms: D057219:Biofouling; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D017953:Isocyanates / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D008863:Microspheres; D010100:Oxygen / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011073:Polyamines / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: C514436:3-isocyanatopropyl trimethoxysilane; D004129:Dimethylpolysiloxanes; D017953:Isocyanates; C531249:Poly(amidoamine); D011073:Polyamines; D011108:Polymers; D012821:Silanes; C013830:baysilon; D010100:Oxygen
keywords: Dendrimer; Detection; Microfluidic device; Non-fouling surface; Sensors; Surface modification