title: Surface modification of silk fibroin fabric using layer-by-layer polyelectrolyte deposition and heparin immobilization for small-diameter vascular prostheses.
abstract: There is an urgent need to develop a biologically active implantable small-diameter vascular prosthesis with long-term patency. Silk-fibroin-based small-diameter vascular prosthesis is a promising candidate having higher patency rate; however, the surface modification is indeed required to improve its further hemocompatibility. In this study, silk fibroin fabric was modified by a two-stage process. First, the surface of silk fibroin fabric was coated using a layer-by-layer polyelectrolyte deposition technique by stepwise dipping the silk fibroin fabric into a solution of cationic poly(allylamine hydrochloride) (PAH) and anionic poly(acrylic acid) (PAA) solution. The dipping procedure was repeated to obtain the PAH/PAA multilayers deposited on the silk fibroin fabrics. Second, the polyelectrolyte-deposited silk fibroin fabrics were treated in EDC/NHS-activated low-molecular-weight heparin (LMWH) solution at 4 °C for 24 h, resulting in immobilization of LMWH on the silk fibroin fabrics surface. Scanning electron microscopy, atomic force microscopy, and energy-dispersive X-ray data revealed the accomplishment of LMWH immobilization on the polyelectrolyte-deposited silk fibroin fabric surface. The higher the number of PAH/PAA coating layers on the silk fibroin fabric, the more surface hydrophilicity could be obtained, resulting in a higher fetal bovine serum protein and platelets adhesion resistance properties when tested in vitro. In addition, compared with untreated sample, the surface-modified silk fibroin fabrics showed negligible loss of bursting strength and thus reveal the acceptability of polyelectrolytes deposition and heparin immobilization approach for silk-fibroin-based small-diameter vascular prostheses modification.
authors: Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University , 2999 North Renmin Road, Songjiang, Shanghai 201620, China.
authors: M Fazley
authors: MF
authors: Elahi
authors: Guoping
authors: G
authors: Guan
authors: Lu
authors: L
authors: Wang
authors: Xinzhe
authors: X
authors: Zhao
authors: Fujun
authors: F
authors: Wang
authors: Martin W
authors: MW
authors: King
mesh_terms: D004573:Electrolytes / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D008970:Molecular Weight; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D047011:Silk; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D011108:Polymers; D047011:Silk; D006493:Heparin; D005349:Fibroins