title: Transcriptional Profiling of Cutaneous MRGPRD Free Nerve Endings and C-LTMRs.
abstract: Cutaneous C-unmyelinated MRGPRD+free nerve endings and C-LTMRs innervating hair follicles convey two opposite aspects of touch sensation: a sensation of pain and a sensation of pleasant touch. The molecular mechanisms underlying these diametrically opposite functions are unknown. Here, we used a mouse model that genetically marks C-LTMRs and MRGPRD+neurons in combination with fluorescent cell surface labeling, flow cytometry, and RNA deep-sequencing technology (RNA-seq). Cluster analysis of RNA-seq profiles of the purified neuronal subsets revealed 486 and 549 genes differentially expressed in MRGPRD-expressing neurons and C-LTMRs, respectively. We validated 48 MRGPD- and 68 C-LTMRs-enriched genes using a triple-staining approach, and the Cav3.3 channel, found to be exclusively expressed in C-LTMRs, was validated using electrophysiology. Our study greatly expands the molecular characterization of C-LTMRs and suggests that this particular population of neurons shares some molecular features with Aβ and Aδ low-threshold mechanoreceptors.
authors: Aix-Marseille-Université, CNRS, Institut de Biologie du Développement de Marseille, UMR 7288, Case 907, 13288 Marseille Cedex 09, France.
authors: Ana
authors: A
authors: Reynders
authors: Aix-Marseille-Université, CNRS, Institut de Biologie du Développement de Marseille, UMR 7288, Case 907, 13288 Marseille Cedex 09, France.
authors: Annabelle
authors: A
authors: Mantilleri
authors: Aix-Marseille-Université, CNRS, Institut de Biologie du Développement de Marseille, UMR 7288, Case 907, 13288 Marseille Cedex 09, France.
authors: Pascale
authors: P
authors: Malapert
authors: Laboratories of Excellence, Ion Channel Science and Therapeutics, Institut de Génomique Fonctionnelle, UMR 5203, CNRS, U1191, INSERM, Université de Montpellier, 141 Rue de la Cardonille, 34094 Montpellier Cedex 05, France.
authors: Stéphanie
authors: S
authors: Rialle
authors: Laboratories of Excellence, Ion Channel Science and Therapeutics, Institut de Génomique Fonctionnelle, UMR 5203, CNRS, U1191, INSERM, Université de Montpellier, 141 Rue de la Cardonille, 34094 Montpellier Cedex 05, France.
authors: Sabine
authors: S
authors: Nidelet
authors: Laboratories of Excellence, Ion Channel Science and Therapeutics, Institut de Génomique Fonctionnelle, UMR 5203, CNRS, U1191, INSERM, Université de Montpellier, 141 Rue de la Cardonille, 34094 Montpellier Cedex 05, France.
authors: Sophie
authors: S
authors: Laffray
authors: Aix-Marseille-Université, CNRS, Institut de Biologie du Développement de Marseille, UMR 7288, Case 907, 13288 Marseille Cedex 09, France.
authors: Corinne
authors: C
authors: Beurrier
authors: Laboratories of Excellence, Ion Channel Science and Therapeutics, Institut de Génomique Fonctionnelle, UMR 5203, CNRS, U1191, INSERM, Université de Montpellier, 141 Rue de la Cardonille, 34094 Montpellier Cedex 05, France. Electronic address: emmanuel.bourinet@igf.cnrs.fr.
authors: Emmanuel
authors: E
authors: Bourinet
authors: Aix-Marseille-Université, CNRS, Institut de Biologie du Développement de Marseille, UMR 7288, Case 907, 13288 Marseille Cedex 09, France. Electronic address: aziz.moqrich@univ-amu.fr.
authors: Aziz
authors: A
authors: Moqrich