title: Quantitative PCR analysis of functional genes in iron-rich microbial mats at an active hydrothermal vent system (Lō'ihi Seamount, Hawai'i).
abstract: The chemolithotrophic Zetaproteobacteria represent a novel class of Proteobacteria which oxidize Fe(II) to Fe(III) and are the dominant bacterial population in iron-rich microbial mats. Zetaproteobacteria were first discovered at Lō'ihi Seamount, located 35 km southeast off the big island of Hawai'i, which is characterized by low-temperature diffuse hydrothermal venting. Novel nondegenerate quantitative PCR (qPCR) assays for genes associated with microbial nitrogen fixation, denitrification, arsenic detoxification, Calvin-Benson-Bassham (CBB), and reductive tricarboxylic acid (rTCA) cycles were developed using selected microbial mat community-derived metagenomes. Nitrogen fixation genes were not detected, but all other functional genes were present. This suggests that arsenic detoxification and denitrification processes are likely cooccurring in addition to two modes of carbon fixation. Two groups of microbial mat community types were identified by terminal restriction fragment length polymorphism (T-RFLP) and were further described based on qPCR data for zetaproteobacterial abundance and carbon fixation mode preference. qPCR variance was associated with mat morphology but not with temperature or sample site. Geochemistry data were significantly associated with sample site and mat morphology. Together, these qPCR assays constitute a functional gene signature for iron microbial mat communities across a broad array of temperatures, mat types, chemistries, and sampling sites at Lō'ihi Seamount.
authors: Department of Biology, Western Washington University, Bellingham, Washington, USA.
authors: Kelsey J
authors: KJ
authors: Jesser
authors: Department of Biology, Western Washington University, Bellingham, Washington, USA.
authors: Heather
authors: H
authors: Fullerton
authors: Department of Biology, Western Washington University, Bellingham, Washington, USA.
authors: Kevin W
authors: KW
authors: Hager
authors: Department of Biology, Western Washington University, Bellingham, Washington, USA cmoyer@hydro.biol.wwu.edu.
authors: Craig L
authors: CL
authors: Moyer
mesh_terms: D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D060086:Hydrothermal Vents / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020560:Proteobacteria / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D002244:Carbon