title: Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication.
abstract: The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.
authors: a Department of Biology ; University of York ; York , UK.
authors: Emma L
authors: EL
authors: Hesketh
authors: John R P
authors: JR
authors: Knight
authors: Rosemary H C
authors: RH
authors: Wilson
authors: James P J
authors: JP
authors: Chong
authors: Dawn
authors: D
authors: Coverley
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D003850:Deoxyribonuclease I / Q000378:metabolism; D016193:G1 Phase / Q000187:drug effects; D051379:Mice; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism*; D015530:Nuclear Matrix / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D016196:S Phase / Q000187:drug effects; D013388:Succinimides / Q000494:pharmacology
chemical_list: D002843:Chromatin; D013388:Succinimides; D003850:Deoxyribonuclease I; D064110:Minichromosome Maintenance Proteins; C011240:dithiobis(succinimidylpropionate)
keywords: DNA replication; MCM, Minichromosome maintenance; MCM2-7, nuclear matrix; NM, Nuclear matrix; cell cycle; initiation; minichromosome maintenance complex