title: Bioactivity of coatings formed on Ti-13Nb-13Zr alloy using plasma electrolytic oxidation.
abstract: In this work, we investigated the bioactivity of anodic oxide coatings on Ti-13Nb-13Zr alloy by plasma electrolytic oxidation (PEO) in solutions containing Ca and P. The bioactive properties of the films were determined by immersion in simulated body fluid (SBF), and their biocompatibility was examined using adult human bone marrow derived mesenchymal stem cells (hBMSCs). The oxide layers were characterised based on their surface morphology (SEM, AFM, profilometry) as well as on their chemical and phase compositions (EDX, XRF, XRD, XPS). We report that anodic oxidation of Ti-13Nb-13Zr led to the development of relatively thick anodic oxide films that were enriched in Ca and P in the form of phosphate compounds. Furthermore, the treatment generated rough surfaces with a significant amount of open pores. The surfaces were essentially amorphous, with small amounts of crystalline phases (anatase and rutile) being observed, depending on the PEO process parameters. SBF soaking led to the precipitation of small crystals after one week of experiment. During culturing of hBMSCs on the bioactive Ti-13Nb-13Zr surfaces the differentiation of human mesenchymal stem cells toward osteoblasts was promoted, which indicated a potential of the modified materials to improve implant osseointegration.
authors: Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego Street 6, 44-100 Gliwice, Poland.
authors: Maciej
authors: M
authors: Sowa
authors: Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego Street 6, 44-100 Gliwice, Poland.
authors: Magdalena
authors: M
authors: Piotrowska
authors: Faculty of Biology and Earth Sciences, Jagiellonian University, Gronostajowa Street 9, 30-060 Kraków, Poland.
authors: Magdalena
authors: M
authors: Widziołek
authors: Institute of Materials Science, University of Silesia, 75 Pulku Piechoty Street 1a, 41-500 Chorzow, Poland.
authors: Grzegorz
authors: G
authors: Dercz
authors: Faculty of Biology and Earth Sciences, Jagiellonian University, Gronostajowa Street 9, 30-060 Kraków, Poland.
authors: Grzegorz
authors: G
authors: Tylko
authors: Institute of Non-Ferrous Metals, Sowińskiego Street 5, 44-100 Gliwice, Poland.
authors: Tadeusz
authors: T
authors: Gorewoda
authors: Faculty of Biology and Earth Sciences, Jagiellonian University, Gronostajowa Street 9, 30-060 Kraków, Poland.
authors: Anna M
authors: AM
authors: Osyczka
authors: Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego Street 6, 44-100 Gliwice, Poland. Electronic address: wojciech.simka@polsl.pl.
authors: Wojciech
authors: W
authors: Simka
mesh_terms: D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D006801:Humans; D010084:Oxidation-Reduction; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; C090153:titanium alloy (TiNb13Zr13); D014025:Titanium
keywords: Adult mesenchymal stem cells; Anodic oxidation; Osteogenesis; Surface modification; Titanium alloy