title: Calcium phosphate coated Keratin-PCL scaffolds for potential bone tissue regeneration.
abstract: The incorporation of hydroxyapatite (HA) nanoparticles within or on the surface of electrospun polymeric scaffolds is a popular approach for bone tissue engineering. However, the fabrication of osteoconductive composite scaffolds via benign processing conditions still remains a major challenge to date. In this work, a new method was developed to achieve a uniform coating of calcium phosphate (CaP) onto electrospun keratin-polycaprolactone composites (Keratin-PCL). Keratin within PCL was crosslinked to decrease its solubility, before coating of CaP. A homogeneous coating was achieved within a short time frame (~10min) by immersing the scaffolds into Ca(2+) and (PO4)(3-) solutions separately. Results showed that the incorporation of keratin into PCL scaffolds not only provided nucleation sites for Ca(2+) adsorption and subsequent homogeneous CaP surface deposition, but also facilitated cell-matrix interactions. An improvement in the mechanical strength of the resultant composite scaffold, as compared to other conventional coating methods, was also observed. This approach of developing a biocompatible bone tissue engineering scaffold would be adopted for further in vitro osteogenic differentiation studies in the future.
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: xinxinzhao@ntu.edu.sg.
authors: Xinxin
authors: X
authors: Zhao
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore; Institute for Sports Research, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: YLUI001@e.ntu.edu.sg.
authors: Yuan Siang
authors: YS
authors: Lui
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: caleb.chookc@gmail.com.
authors: Caleb Kai Chuen
authors: CKC
authors: Choo
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: M110052@e.ntu.edu.sg.
authors: Wan Ting
authors: WT
authors: Sow
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: CharlotteHuang@ntu.edu.sg.
authors: Charlotte Liwen
authors: CL
authors: Huang
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: KWNG@ntu.edu.sg.
authors: Kee Woei
authors: KW
authors: Ng
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: lptan@ntu.edu.sg.
authors: Lay Poh
authors: LP
authors: Tan
authors: Nanyang Technological University, School of Materials Science & Engineering, Division of Materials Technology, N4.1-01-04a, 50 Nanyang Avenue, Singapore 639798, Singapore; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore. Electronic address: joachimloo@ntu.edu.sg.
authors: Joachim Say Chye
authors: JSC
authors: Loo
mesh_terms: D000327:Adsorption; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry* / Q000627:therapeutic use*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000627:therapeutic use*; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D007633:Keratins / Q000737:chemistry* / Q000627:therapeutic use*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry / Q000627:therapeutic use; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D012996:Solutions; C016240:polycaprolactone; D007633:Keratins; D017886:Durapatite; C020243:calcium phosphate; D002118:Calcium
keywords: Crosslinking; Electrospinning; Hydroxyapatite; Keratin; Surface coating