title: Histopathology and Immunophenotyping of Congenital Lacrimal (Anlage) Fistulae.
abstract: OBJECTIVE
  To report the histopathological and immuno histochemical features of congenital lacrimal fistulae.
  
  
  METHODS
  Retrospective chart review of all patients who underwent a fistulectomy for congenital lacrimal fistulae, over a 3-year period from a single surgeon's (M.J.A.) database, were included in the study. A detailed lacrimal system evaluation was performed, and intraoperative findings were documented. The excised fistulae were studied using hematoxylin and eosin and periodic acid-Schiff staining. Immunophenotyping was performed using CD3, CD5, CD10, and CD20.
  
  
  RESULTS
  Twelve excised fistulae of 12 patients were studied. The mean age at presentations was 5.3 years (range, 3-11 years). The deeper parts of the fistulae were lined with hypertrophied stratified squamous epithelium similar to canalicular tissue in 83.3% (10/12), reflecting its origin from the canalicular tissue, and 16.7% (2/12) originated from the lacrimal sac and were lined with columnar epithelium with areas of squamous metaplasia. The subepithelial areas showed presence of fibrosis and chronic inflammatory infiltrate. The infiltrates were lymphoplasmacytic and were positive for CD3, CD5, and CD20 and negative for CD10 immunostaining.
  
  
  CONCLUSIONS
  Hypertrophied stratified squamous lining was the commonest finding. Immunophenotyping revealed features of chronic inflammatory infiltrate composed of a mixture of both T and B lymphocytes. Histopathological analysis of fistulae may be of adjunctive value in determining the origin of the anlage.
authors: *Dacryology Service and †Ocular Pathology Service, L.V. Prasad Eye Institute, Hyderabad, Andhra Pradesh, India.
authors: Mohammad Javed
authors: MJ
authors: Ali
authors: Dilip Kumar
authors: DK
authors: Mishra
authors: Milind N
authors: MN
authors: Naik
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005355:Fibrosis; D005402:Fistula / Q000151:congenital / Q000276:immunology / Q000473:pathology*; D006801:Humans; D006984:Hypertrophy; D016130:Immunophenotyping; D007766:Lacrimal Apparatus Diseases / Q000151:congenital / Q000276:immunology / Q000473:pathology*; D008297:Male; D010503:Periodic Acid-Schiff Reaction; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D015703:Antigens, CD