title: Probiotics inhibit immune fluctuation in the intestinal mucous layer in rats.
abstract: OBJECTIVE
  Innate lymphocytes play a key role in maintaining the immune bowel barrier, which provides resistance to infection. However, the effects of probiotics on changes in the function of innate lymphocytes in the postoperative intestine remain known.
  
  
  METHODS
  Ninety-six male SD rats were divided into the blank group and the preoperative Lactobacillus intervention group, and parts of the ileum were sampled on days 0, 3, 5 and 7 after partial small bowel resection. Distal ileum samples were evaluated using immunohistochemistry to detect IL-5, IL-13, IL-17a and IL-22.
  
  
  RESULTS
  (1) There were more immune cells in the experimental group than in the blank group on day. (2) After the first 3 days, the levels of IL-22 and IL-5 (p = 0.024) were higher in the experimental group than in the blank group. From the third to the fifth day, the IL-5 and IL-22 levels were higher and the IL-13 and IL-17A levels were lower in the experimental group than in the blank group. From the fifth to the seventh day, the IL-5 and IL-13 levels were lower and the IL-22 and IL17A levels were higher in the experimental group than in the blank group.
  
  
  CONCLUSIONS
  Probiotics stabilize the fluctuation of the intestinal immune system postoperatively.
authors: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors: Tian
authors: T
authors: Weijun
authors: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China. zhttj@live.cn.
authors: Zhang
authors: Z
authors: Teng
mesh_terms: D000818:Animals; D007082:Ileum / Q000276:immunology* / Q000378:metabolism; D007150:Immunohistochemistry; D007155:Immunologic Factors; D018793:Interleukin-13 / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D015848:Interleukin-5 / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D023421:Models, Animal; D011184:Postoperative Period; D019936:Probiotics / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013530:Surgical Wound Infection / Q000517:prevention & control; D000097043:Interleukin-22
chemical_list: D007155:Immunologic Factors; D018793:Interleukin-13; D020381:Interleukin-17; D015848:Interleukin-5; D007378:Interleukins; D000097043:Interleukin-22; C515934:Il17a protein, rat
keywords: Intestinal innate lymphocytes; Perioperative period; Probiotics