title: Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.
abstract: Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-regulated while 2,462 down-regulated) and 3,885 (2,141 up-regulated while 1,744 down-regulated) genes were differentially expressed from DT vs DRT and DRT vs ST. The RNA-Seq results were further verified using qRT-PCR. We found reserve mobilization events were activated before the bud emergence (DT vs DRT) and highlighted after dormancy release (DRT vs ST). Overexpressed genes related to metabolism of auxin, gibberellic acid, cytokinin and barssinosteriod were dominated in DT vs DRT, whereas overexpressed genes involved in metabolism of ethylene, jasmonate and salicylate were prominent in DRT vs ST. Various histone and cyclin isoforms associated genes involved in cell division/cycle were mainly up-regulated in DT vs DRT. Dormancy release process was also companied by stress response and redox regulation, those genes related to biotic stress, cell wall and second metabolism was preferentially overexpressed in DRT vs ST, which might accelerate dormancy breaking and sprout outgrowth. The metabolic processes activated during tuber dormancy release were also supported by plant seed models. These results represented the first comprehensive picture of a large number of genes involved in tuber dormancy release process.
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Bailin
authors: B
authors: Liu
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Ning
authors: N
authors: Zhang
authors: College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Yikai
authors: Y
authors: Wen
authors: College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Xin
authors: X
authors: Jin
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Jiangwei
authors: J
authors: Yang
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: hjsi@gsau.edu.cn.
authors: Huaijun
authors: H
authors: Si
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: wangd@gsau.edu.cn.
authors: Di
authors: D
authors: Wang
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D035281:Plant Tubers / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D011198:Solanum tuberosum / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant
keywords: Dormancy; RNA-seq; Sprouting; Transcriptome; Tuber