title: Targeted fluorescent magnetic nanoparticles for imaging of human breast cancer.
abstract: Magnetic nanoclusters coated with ruthenium (II) complexes doped with silica (fluorescent magnetic nanoparticles or FMNPs) could be used for magnetic resonance imaging (MRI) and optical imaging (OI) of human breast cancer. To achieve the targeting imaging of tumors, the peptide cyclic-arginine-glycine-aspartic acid (RGD) was chosen as the probe for specific targeting integrin αvβ3 over expressed in human breast cancer MDA-MB-231 cells. The cytotoxicity tests in vitro showed little toxicity of the synthesized RGD-FMNPs with the size of 150 nm. The in vivo study also showed no obvious acute toxicity after the injection of RGD-FMNPs in mice bearing MDA-MB-231 tumors. After 24 hours of co-culture with MDA-MB-231 cells, the cellular uptake of RGD-FMNPs significantly increased compared to that of FMNPs. T2-weighted (T2W) MRI demonstrated a negative enhancement in mice injected with RGD-FMNPs approximately three times of that injected with FMNPs (12.867 ± 0.451 ms vs. 4.833 ± 0.513 ms, P < 0.05). The Prussian blue staining results confirmed more RGD-FMNPs accumulated around the tumors than FMNPs. These results demonstrated the potential application of RGD-FMNPs as a targeting molecular probe for detection of breast cancer using MRI and OI. The synthesized RGD-FMNPs could be potentially used for biomedical imaging in the future.
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China ; Department of Radiology, Northwestern University Chicago, IL 60611, USA.
authors: Jing
authors: J
authors: Sun
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China.
authors: Zhao-Gang
authors: ZG
authors: Teng
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China.
authors: Ying
authors: Y
authors: Tian
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China.
authors: Jian-Dong
authors: JD
authors: Wang
authors: Department of Radiology, Northwestern University Chicago, IL 60611, USA.
authors: Yang
authors: Y
authors: Guo
authors: Department of Radiology, Northwestern University Chicago, IL 60611, USA.
authors: Dong-Hyun
authors: DH
authors: Kim
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China ; Department of Radiology, Northwestern University Chicago, IL 60611, USA ; Robert H. Lurie Comprehensive Cancer Center Chicago, IL 60611, USA ; Department of Biomedical Engineering, Northwestern University Evanston, IL 60208, USA.
authors: Andrew C
authors: AC
authors: Larson
authors: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, China.
authors: Guang-Ming
authors: GM
authors: Lu
keywords: Fluorescent magnetic nanoparticles (FMNPs); RGD; breast cancer; magnetic resonance imaging (MRI); optical imaging (OI); ruthenium complexes