title: Sampling, metadata and DNA extraction - important steps in metagenomic studies.
abstract: Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.
authors: Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
authors: Agnieszka
authors: A
authors: Felczykowska
authors: Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
authors: Anna
authors: A
authors: Krajewska
authors: Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
authors: Sylwia
authors: S
authors: Zielińska
authors: Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
authors: Joanna M
authors: JM
authors: Łoś
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D017753:Ecosystem; D056186:Metagenomics; D012722:Sewage
chemical_list: D012722:Sewage; D004247:DNA