title: An ultrasensitive detection of 17β-estradiol using a gold nanoparticle-based fluorescence immunoassay.
abstract: A novel ultrasensitive amplification immunoassay for the determination of 17β-estradiol (E2) is reported based on the nanoparticle signal amplification platform. It involves two types of particles: magnetic microparticles (MMPs) functionalized with an anti-E2 antibody produced in rabbit as a capture probe; double-codified gold nanoparticles (DC-AuNPs) modified with both goat anti-rabbit antibody and SH-dsDNA-biotin as a signal amplifier; and avidin-FITC was added to link to the SH-dsDNA-biotin as a tracer. The competitive reaction of the anti-E2 antibody immobilized on magnetic microparticles with estradiol in the sample solution and with the goat anti-rabbit antibody on double-codified gold nanoparticles results in a complex involving the DC-AuNPs and MMPs. Under optimized conditions, the linear range of E2 is from 1.0 × 10(-5) to 1.0 ng mL(-1), and the detection limit of the assay could reach up to 6.37 × 10(-6) ng mL(-1). It was applied to determine E2 in human urine, with mean percent recoveries in the range of 96.5%-107.4%, and relative standard deviations were below 8.1%.
authors: Shandong Provincial Key Laboratory of Chemical Energy Storage and spectrometry (LC/MS/MS) and gas chromatography-mass Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. shuhaowang@sohu.com.
authors: Lingyun
authors: L
authors: Du
authors: Wei
authors: W
authors: Ji
authors: Yuanfu
authors: Y
authors: Zhang
authors: Chunyan
authors: C
authors: Zhang
authors: Guofu
authors: G
authors: Liu
authors: Shuhao
authors: S
authors: Wang
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004958:Estradiol / Q000652:urine*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005453:Fluorescence; D006041:Goats; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D013438:Sulfhydryl Compounds; C036963:fluorescein isothiocyante avidin; D001360:Avidin; D004958:Estradiol; D001710:Biotin; D006046:Gold; D004247:DNA; D016650:Fluorescein-5-isothiocyanate