title: Effects of culturing media on hepatocytes differentiation using Volvox sphere as co-culturing vehicle.
abstract: Volvox sphere is a unique design to mimic natural volvox consists of a large outer-sphere that contains smaller inner-spheres, which provide three-dimensional (3D) environment to culture cells. The purpose of this study is to co-culture mesenchymal stem cells (MSCs) and AML12 liver cells in Volvox spheres and to evaluate the effects of two media, DMEM and DMEM/F12 on the cultured cells. The results of this study shows that the 3D Volvox sphere can successfully be applied for co-culture of MSCs and AML12 liver cells, and the MSCs are able to differentiate into hepatocyte-like cells expressing hepatocyte-specific markers including albumin (ALB), alpha feto-protein (AFP) and cytokeratin 18 (CK18) mRNA expressions and producing CK18 and ALB proteins. Interestingly, the MSCs expressed higher ALB, AFP and CK18 mRNA expression at the initial 7-day culture by using DMEM, whereas, the MSCs expressed more mRNA expressions from 7-day to 14-day by the usage of DMEM/F12. The result demonstrated that DMEM and DMEM/F12 media could affect MSCs behaviors during a 14-day culture.
authors: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC.
authors: Kun Lieh
authors: KL
authors: Wu
authors: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC.
authors: Siou Han
authors: SH
authors: Chang
authors: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC.
authors: Ioannis
authors: I
authors: Manousakas
authors: Department of Veterinary Medicine, National Chiayi University, Chiayi City, Taiwan, ROC.
authors: Han Hsiang
authors: HH
authors: Huang
authors: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC.
authors: Benjamin
authors: B
authors: Teong
authors: Department of Electric Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC.
authors: Chin Wen
authors: CW
authors: Chuang
authors: Department of Biomedical Engineering, I-Shou University, Kaohsiung City, Taiwan, ROC. Electronic address: smkuo@isu.edu.tw.
authors: Shyh Ming
authors: SM
authors: Kuo
mesh_terms: D000418:Albumins / Q000235:genetics; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000378:metabolism*; D004867:Equipment Design; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D053538:Keratin-18 / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D017207:Rats, Sprague-Dawley; D044446:Volvox / Q000737:chemistry / Q000166:cytology; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D000418:Albumins; D003470:Culture Media; D053538:Keratin-18; D012333:RNA, Messenger; D000509:alpha-Fetoproteins
keywords: Co-culture; Media; Mesenchymal stem cell; Volvox sphere