title: Dual high-resolution α-glucosidase and radical scavenging profiling combined with HPLC-HRMS-SPE-NMR for identification of minor and major constituents directly from the crude extract of Pueraria lobata.
abstract: The crude methanol extract of Pueraria lobata was investigated by dual high-resolution α-glucosidase inhibition and radical scavenging profiling combined with hyphenated HPLC-HRMS-SPE-NMR. Direct analysis of the crude extract without preceding purification was facilitated by combining chromatograms from two analytical-scale HPLC separations of 120 and 600 μg on-column, respectively. High-resolution α-glucosidase and radical scavenging profiles were obtained after microfractionation of the eluate in 96-well microplates. This allowed full bioactivity profiling of individual peaks in the HPLC chromatogram of the crude methanol extract. Subsequent HPLC-HRMS-SPE-NMR analysis allowed identification of 21 known compounds in addition to two new compounds, i.e., 3'-methoxydaidzein 8-C-[α-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside and 6″-O-malonyl-3'-methoxydaidzin, as well as an unstable compound tentatively identified as 3'-de-O-methylpuerariafuran.
authors: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors: Bingrui
authors: B
authors: Liu
authors: Kenneth T
authors: KT
authors: Kongstad
authors: Sun
authors: S
authors: Qinglei
authors: Nils T
authors: NT
authors: Nyberg
authors: Anna K
authors: AK
authors: Jäger
authors: Dan
authors: D
authors: Staerk
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*; D052616:Solid Phase Extraction; D000520:alpha-Glucosidases / Q000187:drug effects*
chemical_list: C000600241:3'-methoxydaidzein 8-C-(alpha-D-apiofuranosyl-(1-6))-beta-D-glucopyranoside; C000600242:6'-O-malonyl-3'-methoxydaidzin; D005960:Glucosides; D065089:Glycoside Hydrolase Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; D000520:alpha-Glucosidases