title: Inhibitory effect of chondroitin sulfate oligosaccharides on bovine testicular hyaluronidase.
abstract: Hyaluronan and chondroitin sulfates are prominent components of the extracellular matrices of animal tissues; however, their functions in relation to their oligosaccharide structures have not yet been fully elucidated. The oligosaccharides of hyaluronan and chondroitin sulfate were prepared and used to investigate their effects on the hydrolysis and transglycosylation reactions of bovine testicular hyaluronidase when hyaluronan was used as a substrate. Hydrolysis and transglycosylation activities were assessed in independent reaction systems by analyzing the products by HPLC. The hydrolysis and transglycosylation reactions of bovine testicular hyaluronidase were dose-dependently inhibited by chondroitin sulfate oligosaccharides, but not by hyaluronan or chondroitin oligosaccharides. A kinetic analysis of the hydrolysis reaction using hyaluronan octasaccharide revealed that the inhibition mode by chondroitin sulfate oligosaccharides was competitive.
authors: Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan; Department of Glycobiochemistry, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. Electronic address: kaki@cc.hirosaki-u.ac.jp.
authors: Ikuko
authors: I
authors: Kakizaki
authors: Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors: Hideyo
authors: H
authors: Koizumi
authors: Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors: Fengchao
authors: F
authors: Chen
authors: Department of Glycobiochemistry, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors: Masahiko
authors: M
authors: Endo
mesh_terms: D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D002809:Chondroitin Sulfates; D006821:Hyaluronoglucosaminidase
keywords: Chondroitin sulfate; Hyaluronan; Hyaluronidase; Inhibitor; Oligosaccharide