title: A sensitive glucose biosensor based on Ag@C core-shell matrix.
abstract: Nano-Ag particles were coated with colloidal carbon (Ag@C) to improve its biocompatibility and chemical stability for the preparation of biosensor. The core-shell structure was evidenced by transmission electron microscope (TEM) and the Fourier transfer infrared (FTIR) spectra revealed that the carbon shell is rich of function groups such as -OH and -COOH. The as-prepared Ag@C core-shell structure can offer favorable microenvironment for immobilizing glucose oxidase and the direct electrochemistry process of glucose oxidase (GOD) at Ag@C modified glassy carbon electrode (GCE) was realized. The modified electrode exhibited good response to glucose. Under optimum experimental conditions the biosensor linearly responded to glucose concentration in the range of 0.05-2.5mM, with a detection limit of 0.02mM (S/N=3). The apparent Michaelis-Menten constant (KM(app)) of the biosensor is calculated to be 1.7mM, suggesting high enzymatic activity and affinity toward glucose. In addition, the GOD-Ag@C/Nafion/GCE shows good reproducibility and long-term stability. These results suggested that core-shell structured Ag@C is an ideal matrix for the immobilization of the redox enzymes and further the construction of the sensitive enzyme biosensor.
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors: Xuan
authors: X
authors: Zhou
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors: Xingxin
authors: X
authors: Dai
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors: Jianguo
authors: J
authors: Li
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, PR China. Electronic address: yumeilong@suda.edu.cn.
authors: Yumei
authors: Y
authors: Long
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address: liweifeng@suda.edu.cn.
authors: Weifeng
authors: W
authors: Li
authors: College of Chemistry, Chemical engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, PR China.
authors: Yifeng
authors: Y
authors: Tu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D012834:Silver; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose
keywords: Ag@C core–shell structure; Biosensor; Electrochemistry; Glucose oxidase