title: MicroRNA-31 Promotes Skin Wound Healing by Enhancing Keratinocyte Proliferation and Migration.
abstract: Wound healing is a basic biological process restoring the integrity of the skin. The role of microRNAs during this process remains largely unexplored. By using an in vivo human skin wound healing model, we show here that the expression of miR-31 is gradually upregulated in wound edge keratinocytes in the inflammatory (1 day after injury) through the proliferative phase (7 days after injury) in comparison with intact skin. In human primary keratinocytes, overexpression of miR-31 promoted cell proliferation and migration, whereas inhibition of miR-31 had the opposite effects. Moreover, we identified epithelial membrane protein 1 (EMP-1) as a direct target of miR-31 in keratinocytes. The expression of EMP-1 in the skin was negatively correlated with the level of miR-31 during wound healing. Silencing of EMP-1 mimicked the effects of overexpression of miR-31 on keratinocyte proliferation and migration, indicating that EMP-1 is a critical target mediating the functions of miR-31 in keratinocytes. Finally, we demonstrated that transforming growth factor-β2, which is highly expressed in skin wounds, upregulated miR-31 expression in keratinocytes. Collectively, we identify miR-31 as a key regulator for promoting keratinocyte proliferation and migration during wound healing.
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Dongqing
authors: D
authors: Li
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: X I
authors: XI
authors: Li
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors: Aoxue
authors: A
authors: Wang
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Florian
authors: F
authors: Meisgen
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Andor
authors: A
authors: Pivarcsi
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Enikö
authors: E
authors: Sonkoly
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Mona
authors: M
authors: Ståhle
authors: Molecular Dermatology Research Group, Unit of Dermatology and Venereology, Centre for Molecular Medicine (CMM), Department of Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: ning.xu@ki.se.
authors: Ning Xu
authors: NX
authors: Landén
mesh_terms: D000328:Adult; D001706:Biopsy; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D007249:Inflammation; D015603:Keratinocytes / Q000166:cytology*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism; D015854:Up-Regulation; D014945:Wound Healing; D055815:Young Adult
chemical_list: C534612:MIRN31 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D053781:Transforming Growth Factor beta2; C097051:epithelial membrane protein-1