title: Cloning and analysis of expression patterns and transcriptional regulation of RghBNG in response to plant growth regulators and abiotic stresses in Rehmannia glutinosa.
abstract: RghBNG, a gene of unknown function, was cloned from Rehmannia glutinosa by reverse transcription PCR and rapid amplification of cDNA ends. The full-length cDNA of RghBNG was 548 bp with a282-bp open reading frame. It encoded a polypeptide of 93 amino acids with a predicted molecular weight of 10.5 kDa and a theoretical isoelectric point of 9.25. Bioinformatics analysis indicated that RghBNG had no homology to any known plant genes, whereas the RghBNG polypeptide was highly similar to other plant proteins and possessed one conserved B12D protein family functional domain. Phylogenetic analysis revealed that RghBNG encoded for a dicot protein. RghBNG spatial and temporal expression patterns and responses to abiotic stresses and plant growth regulators were investigated by qRT-PCR. RghBNG transcripts were detected in roots, stems, leaves, petals, receptacles, stamens and pistils with the highest and lowest levels respectively observed in petals and leaves of mature plants. Additionally, RghBNG transcripts were detected at three developmental stages of roots, stems and leaves; the highest levels were observed in roots at seedling stage; Transcript levels changed to varying degrees in different tissues and stages; We also studied the effects of abiotic stress and plant growth regulators in roots and leaves. RghBNG expression was significantly increased (p < 0.01) by chromium, gibberellic acid and NaCl, with the highest levels induced by chromium stress; In contrast, 6-benzyladenine reduced expression. These results strongly suggest that RghBNG is involved in R. glutinosa growth, development and response to plant growth regulators and abiotic stresses.
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.|Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Yanqing
authors: Y
authors: Zhou
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Yonghua
authors: Y
authors: Zhang
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Jun
authors: J
authors: Wei
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Yu
authors: Y
authors: Zhang
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Jingyun
authors: J
authors: Li
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Wanshen
authors: W
authors: Wang
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Hongying
authors: H
authors: Duan
authors: College of Life Sciences, Henan Normal University, No.46 Jianshe Road, Xinxiang, 453007, Henan China.
authors: Juanjuan
authors: J
authors: Chen
keywords: Cloning and expression; qRT-PCR; Abiotic stress; Plant growth regulator; Rehmannia glutinosa; RghBNG gene