title: Chemically reactive supramolecular hydrogel coupled with a signal amplification system for enhanced analyte sensitivity.
abstract: Multicomponent supramolecular hydrogels are constructed for sensitive, naked-eye detection of small-molecule biomarkers. A dendritic self-immolative molecule and the corresponding enzyme as a signal amplification system were stably embedded in a hydrogen peroxide (H2O2)-responsive supramolecular hydrogel (BPmoc-F3), together with other enzymes. The nanostructure and mechanical strength of the hybrid BPmoc-F3 gel were not substantially diminished by incorporation of these multiple components in the absence of target biomarkers, but could be destroyed by addition of the biomarker through the multiple enzymatic and chemical cascade reactions operating in combination within the gel matrix. The sensitivity to biomarkers such as H2O2, glucose, and uric acid, detected by gel-sol transition, was significantly enhanced by the signal amplification system. An array chip consisting of these multicomponent hydrogels enabled the detection of the level of hyperuricemia disease in human plasma samples.
authors: Department of Synthetic Chemistry and Biological Chemistry, Kyoto University , Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan.
authors: Tatsuyuki
authors: T
authors: Yoshii
authors: Shoji
authors: S
authors: Onogi
authors: Hajime
authors: H
authors: Shigemitsu
authors: Itaru
authors: I
authors: Hamachi
mesh_terms: D015415:Biomarkers; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D060326:Chemistry Techniques, Synthetic; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000032:analysis; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D050879:Sarcosine Oxidase / Q000737:chemistry; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis / Q000097:blood
chemical_list: D015415:Biomarkers; D020100:Hydrogels; D014527:Uric Acid; D006861:Hydrogen Peroxide; D050879:Sarcosine Oxidase; D014503:Urate Oxidase; D005947:Glucose