title: Replication of IMR-32-adapted JC virus clones in human embryonic kidney cells.
abstract: It has been difficult to study JCV replication because of its restricted host range. In this study, JCV replication was examined using different clones in 293 cells. RT-PCR assay revealed that large T antigen expression in cells transfected with IMR-32-adapted JCVs was significantly greater than in those transfected with Mad-1 or CY. DNA replication assay and viral load verified that the IMR-32-adapted JCVs were replication-competent in 293 cells, but not Mad-1 or CY JCVs. These results suggest that a 293 culture system with IMR-32-adapted JCVs may be a useful tool for assessing replication of JCV in vitro.
authors: Department of Infectious Diseases, Kobe Institute of Health, 4-6 Minatojima-Nakamachi, Chuo-ku, Kobe, 650-0046.
authors: Souichi
authors: S
authors: Nukuzuma
authors: Shigeki
authors: S
authors: Sugiura
authors: Kazuo
authors: K
authors: Nakamichi
authors: Masanori
authors: M
authors: Kameoka
authors: Chiyoko
authors: C
authors: Nukuzuma
authors: Takafumi
authors: T
authors: Tasaki
authors: Tsutomu
authors: T
authors: Takegami
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000502:physiology*; D007668:Kidney / Q000196:embryology / Q000821:virology*; D027601:Polyomavirus Infections / Q000821:virology*; D019562:Viral Load; D014776:Virus Cultivation; D014779:Virus Replication
keywords: 293 cells; DNA replication assay; IMR-32-adapted JCVs; large T antigen