title: The role of Rab6 GTPase in the maturation of phagosome against Staphylococcus aureus.
abstract: Phagocytosis, an evolutionarily conserved process in animals, plays a central role in host defense against pathogens. As reported, Rab6 GTPase was involved in the regulation of hemocytic phagocytosis in invertebrates. However, the role of Rab6 GTPase in mammalian phagocytosis remains to be addressed. In this study, the results showed that Rab6 GTPase took great effects on phagocytosis of mouse leukemic monocyte macrophages (RAW 264.7 cells). It was revealed that Rab6 GTPase was required during the phagosome maturation by its interaction with bicaudal-D1 (BICD1) protein. Further data presented that the Rab6 GTPase-regulated phagocytosis could influence the proliferation of Staphylococcus aureus in macrophages. Therefore, our study demonstrated a novel insight into the mechanism of regulation of mammalian phagocytosis by Rab6 GTPase and a novel strategy for the control of Staphylococcus aureus.
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Yulei
authors: Y
authors: Chen
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Chunxia
authors: C
authors: Jiang
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Min
authors: M
authors: Jin
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Yi
authors: Y
authors: Gong
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: zxb0812@zju.edu.cn.
authors: Xiaobo
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D055785:Gene Knockdown Techniques; D007113:Immunity, Innate; D008264:Macrophages / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D014162:Transfection; D020691:rab GTP-Binding Proteins / Q000276:immunology*
chemical_list: C094135:Rab6 protein; D020691:rab GTP-Binding Proteins
keywords: Mammalian; Pathogen; Phagocytosis; Phagosome maturation; Rab6