title: Long non-coding RNA-DANCR in human circulating monocytes: a potential biomarker associated with postmenopausal osteoporosis.
abstract: Osteoporosis is a common disease characterized by low bone mineral density (BMD) and low trauma fractures, mainly resulting from exceeding bone resorption by osteoclasts over bone formation by osteoblasts. Circulating monocytes are directly involved in osteoclastogenesis, and lncRNAs are believed to be involved in the osteoblast differentiation. However, no study has been conducted to identify the roles of lncRNA in circulating monocytes associated with human osteoporosis. In this study, we found significant upregulation of DANCR in the blood mononuclear cells (MNCs) from low-BMD patients with the qRT-PCR analyses. We further found that DANCR promoted the expression of IL6 and TNF-α at both mRNA level and protein level in MNCs. After deletion of DANCR with siRNAs, the levels of IL6 and TNF-α are decreased in the MNCs from low-BMD postmenopausal women. Moreover, DANCR level was correlated with IL6 and TNF-α in postmenopausal women with low BMD. Furthermore, we found that DANCR-induced IL6 and TNF-α in MNCs had bone-resorbing activity. These results indicate that DANCR is involved in the pathology of osteoporosis and may be as a biomarker for postmenopausal osteoporosis.
authors: a Department of Orthopedic Surgery, The 1st Affiliated Hospital, College of Medicine , Zhejiang University , Hangzhou , China.
authors: Xiang
authors: X
authors: Tong
authors: Peng-cheng
authors: PC
authors: Gu
authors: San-zhong
authors: SZ
authors: Xu
authors: Xiang-jin
authors: XJ
authors: Lin
mesh_terms: D000368:Aged; D015519:Bone Density; D001862:Bone Resorption / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D015663:Osteoporosis, Postmenopausal / Q000097:blood / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000032:analysis / Q000097:blood / Q000235:genetics / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C000625902:DANCR long noncoding RNA, human; D005819:Genetic Markers; C508600:IL6 protein, human; D015850:Interleukin-6; D062085:RNA, Long Noncoding; D014409:Tumor Necrosis Factor-alpha
keywords: DANCR; blood mononuclear cells; bone mineral density; lncRNAs; osteoporosis