title: Tip-enhanced fluorescence with radially polarized illumination for monitoring loop-mediated isothermal amplification on hepatitis C virus cDNA.
abstract: A tip nanobiosensor for monitoring DNA replication was presented. The effects of excitation power and polarization on tip-enhanced fluorescence (TEF) were assessed with the tip immersed in fluorescein isothiocyanate solution first. The photon count rose on average fivefold with radially polarized illumination at 50 mW. We then used polymerase-functionalized tips for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA. The amplicon-SYBR® Green I complex was detected and compared to real-time loop-mediated isothermal amplification. The signals of the reaction using 4 and 0.004 ng∕μl templates were detected 10 and 30 min earlier, respectively. The results showed the potential of TEF in developing a nanobiosensor for real-time DNA amplification.
authors: National Taiwan University, Institute of Biomedical Electronics and Bioinformatics, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors: Shih-Chung
authors: SC
authors: Wei
authors: National Taiwan University, Institute of Biomedical Engineering, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors: Tsung-Liang
authors: TL
authors: Chuang
authors: National Taiwan University, Institute of Biomedical Engineering, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors: Da-Shin
authors: DS
authors: Wang
authors: National Taiwan University, Institute of Biomedical Engineering, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors: Hui-Hsin
authors: HH
authors: Lu
authors: University of Waterloo, Department of Chemical Engineering, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
authors: Frank X
authors: FX
authors: Gu
authors: National Taiwan University, Institute of Biomedical Electronics and Bioinformatics, No. 1, Section 4, Roosevelt Road, Taipei 10617, TaiwandNational Taiwan University, Molecular Imaging Center, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors: Kung-Bin
authors: KB
authors: Sung
authors: National Taiwan University, Institute of Biomedical Electronics and Bioinformatics, No. 1, Section 4, Roosevelt Road, Taipei 10617, TaiwanbNational Taiwan University, Institute of Biomedical Engineering, No. 1, Section 4, Roosevelt Road, Taipei 10617, Tai.
authors: Chii-Wann
authors: CW
authors: Lin
mesh_terms: D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D016174:Hepacivirus / Q000235:genetics*; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012367:RNA, Viral; D019793:Fluorescein