title: Cryptococcus gattii sero-mating type allelic pattern determined by multiplex PCR.
abstract: Molecular methods to differentiate serotypes, mating types and molecular types of Cryptococcus neoformans and C. gattii are important tools to understand epidemiology and pathogenesis of these pathogens. In this study, a multiplex polymerase chain reaction (PCR) approach was applied to sero-mating typing of C. gattii strains. Four pairs of primers were designed to target 4 allele-specific genes located in the mating-type locus. Twenty-three C. gattii strains, presenting different mating types and serotypes, were tested to validate the method. The method was able to identify all sero-mating allelic patterns including hybrid combinations, and therefore, it represents a simple one-step PCR for sero-mating typing of C. gattii strains.
authors: Lab. Micologia Medica, Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milano, Italy. Electronic address: massimo.cogliati@unimi.it.
authors: M
authors: M
authors: Cogliati
authors: Lab. Micologia Medica, Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milano, Italy.
authors: R
authors: R
authors: D'Amicis
authors: Lab. Micologia Medica, Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milano, Italy.
authors: A M
authors: AM
authors: Tortorano
mesh_terms: D000483:Alleles; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D012703:Serotyping / Q000379:methods
keywords: Cryptococcus gattii; mating type; molecular type; multiplex PCR; serotype