title: Rapamycin protects cardiomyocytes against anoxia/reoxygenation injury by inducing autophagy through the PI3k/Akt pathway.
abstract: The purpose of this study was to investigate the potential cardioprotection roles of Rapamycin in anoxia/reoxygenation (A/R) injury of cardiomyocytes through inducing autophagy, and the involvement of PI3k/Akt pathway. We employed simulated A/R of neonatal rat ventricular myocytes (NRVM) as an in vitro model of ischemial/reperfusion (I/R) injury to the heart. NRVM were pretreated with four different concentrations of Rapamycin (20, 50, 100, 150 μmol/L), and pretreated with 10 mmol/L 3-methyladenine (3MA) for inhibiting autophagy during A/R. Then, Western blot analysis was used to examine variation in the expression of LC3-II, LC3-I, Bim, caspase-3, p-PI3KI, PI3KI, p-Akt and Akt. In our model, Rapamycin had a preferential action on autophagy, increasing the expression of LC3-II/LC3-I, whereas decreasing the expression of Bim and caspase-3. Moreover, our results also demonstrated that Rapamycin inhibited the activation of p-PI3KI and enhanced the activation of p-Akt. It is concluded that Rapamycin has a cardioprotection effect by inducing autophagy in a concentration-dependent manner against apopotosis through PI3K/Akt signaling pathway during A/R in NRVM.
authors: Jiangxi Key Laboratory of Molecular Medicine, Nanchang University, Nanchang, 330006, China.|Department of Cardiovascular Medicine, Nanchang University, Nanchang, 330006, China.
authors: Lu-Qiao
authors: LQ
authors: Wang
authors: Jiangxi Key Laboratory of Molecular Medicine, Nanchang University, Nanchang, 330006, China. xiaoshumenfan@126.com.|Department of Cardiovascular Medicine, Nanchang University, Nanchang, 330006, China. xiaoshumenfan@126.com.
authors: Xiao-Shu
authors: XS
authors: Cheng
authors: Jiangxi Key Laboratory of Molecular Medicine, Nanchang University, Nanchang, 330006, China.
authors: Cha-Hua
authors: CH
authors: Huang
authors: Department of Clinical Laboratory, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Bo
authors: B
authors: Huang
authors: Jiangxi Key Laboratory of Molecular Medicine, Nanchang University, Nanchang, 330006, China.
authors: Qian
authors: Q
authors: Liang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D032383:Myocytes, Cardiac / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000517:prevention & control*; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020123:Sirolimus