title: A study in familial hypercholesterolemia suggests reduced methylomic plasticity in men with coronary artery disease.
abstract: OBJECTIVE
  To assess whether DNA methylation is associated with coronary artery disease (CAD).
  
  
  METHODS
  An epigenome-wide analysis has been performed on leucocytes from familial hypercholesterolemic (FH) men with (n = 6) or without CAD (n = 6). The results were replicated in an extended sample of FH men (n = 61) and in non-FH men (n = 100) for two of the top differentially methylated loci.
  
  
  RESULTS
  FH men with CAD had significantly more hypomethylated and hypermethylated loci and showed less DNA methylation level variability compared with men without CAD (p < 0.001). Moreover, COL14A1 and MMP9 DNA methylation levels were associated with CAD, age of onset of CAD or CAD risk factors.
  
  
  CONCLUSIONS
  These results suggest that epigenome-wide changes are associated with CAD occurrence in men.
authors: Department of Biochemistry, Université de Sherbrooke, University-Affiliated Chicoutimi Hospital, 305 rue St-Vallier, Saguenay, Québec G7H 5H6, Canada.
authors: Simon-Pierre
authors: SP
authors: Guay
authors: Diane
authors: D
authors: Brisson
authors: Patrick
authors: P
authors: Mathieu
authors: Yohan
authors: Y
authors: Bossé
authors: Daniel
authors: D
authors: Gaudet
authors: Luigi
authors: L
authors: Bouchard
mesh_terms: D003094:Collagen / Q000235:genetics; D003324:Coronary Artery Disease / Q000150:complications / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods; D063990:Gene Ontology; D055106:Genome-Wide Association Study; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000150:complications* / Q000235:genetics; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D008875:Middle Aged
chemical_list: C054447:COL14A1 protein, human; D006023:Glycoproteins; D003094:Collagen; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: DNA methylation; arterial remodeling; candidate genes; cardiovascular diseases; epigenome-wide association study; gene expression; missing heritability