title: Changes in the BDNF-immunopositive cell population of neocortical layers I and II/III after focal cerebral ischemia in rats.
abstract: Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family and is widely distributed in the central nervous system, including the cerebral cortex. BDNF plays an important role in normal neural development, survival of existing neurons, and activity-dependent neuroplasticity. BDNF can also be neuroprotective and evoke neurogenesis in certain pathological conditions, such as cerebral ischemia. Neocortical layer I is an important region that can integrate feedforward and feedback information from other cortical areas and subcortical regions. In addition, it has recently been proposed as a possible source of neuronal progenitor cells after ischemia. Therefore, we investigated changes in the BDNF-immunoreactive cell population of neocortical layers I and II/III after middle cerebral artery occlusion (MCAO)-induced cerebral ischemia in rats. In unaffected condition, the number of BDNF(+) cells in layer I was significantly less than in layer II/III in the cingulate cortex and in the motor and sensory areas. The increase in the number of BDNF(+) cells in layer I 8 days after MCAO was more remarkable than layer II/III, in all regions except the area of cingulate cortex farthest from the infarct core. Only BDNF(+)-Ox-42(+) cells showed a tendency to increase consistently toward the infarct core in both layers I and II/III, implying a major source of BDNF for response to ischemic injury. The present study suggests that some beneficial effects during recovery from ischemic injury, such as increased supportive microglia/macrophages, occur owing to a sensitive response of BDNF in layer I.
authors: Department of Rehabilitation Science, Inje University, Gimhae 621-749, Republic of Korea; U-Healthcare & Anti-aging Research Center, Inje University, Gimhae 621-749, Republic of Korea.
authors: Yongwon
authors: Y
authors: Choi
authors: Department of Biological Sciences, Institute of Basic Science, Inje University, Gimhae 621-749, Republic of Korea.
authors: Sung Goo
authors: SG
authors: Kang
authors: Department of Occupational Therapy, Inje University, Gimhae 621-749, Republic of Korea; U-Healthcare & Anti-aging Research Center, Inje University, Gimhae 621-749, Republic of Korea. Electronic address: kamlapa@inje.ac.kr.
authors: Kyung-Yoon
authors: KY
authors: Kam
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D019579:Neocortex / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor
keywords: BDNF; Ischemia; Macrophage; Microglia; Neocortical layer I