title: Dectin-1 deficiency does not affect atherosclerosis development in mice.
abstract: OBJECTIVE
  Recent data suggest the involvement of dectin-1 in atherosclerosis through regulation of local reactive oxygen species production. The aim of the current study was to assess the effect of dectin-1 deficiency on atherosclerotic plaque development.
  
  
  METHODS
  Using immunohistochemistry dectin-1 expression was observed on foamy macrophages in atherosclerotic lesions in mice. Following lethal irradiation LDLR(-/-) mice were reconstituted with bone marrow from either wild type or dectin-1(-/-) mice. After recovery, mice were fed a high fat diet for 9 weeks and atherosclerotic lesions were analyzed.
  
  
  CONCLUSIONS
  Overall, we found no significant differences in plaque size or severity between the groups. Also no differences were observed in granulocyte or macrophage composition of the plaques or in the ability to produce reactive oxygen species by macrophages from both groups. Dectin-1 is dispensable for the development of atherosclerotic lesions in mice.
authors: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: k.szilagyi@sanquin.nl.
authors: Katka
authors: K
authors: Szilagyi
authors: Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands; Department of Pathology and Department of Molecular Genetics, CARIM, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
authors: Marion J J
authors: MJ
authors: Gijbels
authors: Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Saskia
authors: S
authors: van der Velden
authors: Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Sigrid E M
authors: SE
authors: Heinsbroek
authors: Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.
authors: Georg
authors: G
authors: Kraal
authors: Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Menno P J
authors: MP
authors: de Winther
authors: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Timo K
authors: TK
authors: van den Berg
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000235:genetics* / Q000503:physiopathology; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000172:deficiency*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058226:Plaque, Atherosclerotic / Q000235:genetics* / Q000503:physiopathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics; D016897:Respiratory Burst; D014746:Vimentin / Q000378:metabolism; D000099181:Dectin-1
chemical_list: D037181:Lectins, C-Type; D017382:Reactive Oxygen Species; D011973:Receptors, LDL; D014746:Vimentin; D000099181:Dectin-1
keywords: Atherosclerosis; Dectin-1; Macrophages; Respiratory burst