title: CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.
abstract: In this study, DNA arrays have been employed to monitor gene expression patterns in testis of mice exposed to tobacco smoke for 24 weeks and compared to control animals. The results of the analysis revealed significant changes in expression of several genes that may have a role in spermatogenesis. Cdk14 was chosen for further characterization because of a suggested role in the testis and in regulation of Wnt signaling. RT-PCR analysis confirmed down regulation of Cdk14 in mice exposed to cigarette smoke (CS). Cdk14 is expressed in all testicular cells; spermatogonia- and Sertoli-derived cell lines treated with cigarette smoke extract (CSE) in vitro showed down-regulation of CDK14 mRNA and protein levels as well as down-regulation of β-catenin levels. CS-induced down-regulation of CDK14 mRNA and protein levels was also observed in several lung epithelium-derived cell lines including primary normal human bronchial epithelial cells (NHBE), suggesting that the effect is not restricted to the testis. Similar to testicular cells, CS-induced down-regulation of CDK14 in lung cells correlated with decreased levels of β-catenin, a finding suggesting impaired Wnt signaling. In the lungs, CDK14 was localized to the alveolar and bronchial epithelium.
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA.
authors: Daniel
authors: D
authors: Pollack
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA.
authors: Yuxuan
authors: Y
authors: Xiao
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA.
authors: Vibha
authors: V
authors: Shrivasatava
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA.
authors: Avi
authors: A
authors: Levy
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA.
authors: Miriam
authors: M
authors: Andrusier
authors: Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University, New York, NY, USA.
authors: Jeanine
authors: J
authors: D'Armiento
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA; Department of Molecular Pharmacology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA.
authors: Marina K
authors: MK
authors: Holz
authors: Department of Biology, Stern College, Yeshiva University, New York, NY, USA; Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA. Electronic address: vigodner@yu.edu.
authors: Margarita
authors: M
authors: Vigodner
mesh_terms: D000818:Animals; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects; D008297:Male; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012708:Sertoli Cells / Q000187:drug effects / Q000201:enzymology; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects* / Q000235:genetics / Q000378:metabolism; D013093:Spermatogonia / Q000187:drug effects / Q000201:enzymology; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors; D060449:Wnt Signaling Pathway / Q000187:drug effects; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; C495267:CTNNB1 protein, mouse; D012906:Smoke; D051176:beta Catenin; D011494:Protein Kinases; C112141:Pftk1 protein, mouse; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases
keywords: CDK14/PFTK1/PFTAIRE-1; Cigarette smoke; Cyclin Y; DNA arrays; Lungs; Normal human bronchial cells; Spermatogenesis; Spermatogonia; β-Catenin