title: Prevalent and up-regulated vimentin expression in micropapillary components of lung adenocarcinomas and its adverse prognostic significance.
abstract: The factors conferring the increased malignancy on lung adenocarcinoma with micropapillary component (AC-MPC) remain to be elucidated. On proteomics based on 2-dimensional gel electrophoresis, 19 proteins differentially expressed by more than 1.5-fold between AC-MPC and conventional adenocarcinoma (CAC); in particular, vimentin, one of the proteins, was 3.5-fold up-regulated in AC-MPC. Subsequent semi-quantitative investigation by immunohistochemistry with large cohorts comprised 101 AC-MPC and 119 CAC, respectively, of different stages revealed that vimentin was expressed in MPC of 95 (94.1%) AC-MPC and the expression scores were higher than those of well- and moderately differentiated CAC, as well as the background non-MPC of the AC-MPC (P < 0.0001), but not significantly different from those of poorly differentiated CAC (P = 0.561). Even within the AC-MPC entity, higher vimentin expression was correlated with more frequent vascular invasion and more advanced node metastasis (P < 0.02), and multivariate analysis showed that high vimentin expression and worse node statuses were independent indicators of adverse prognosis (P < 0.048). In conclusion, vimentin expression is prevalent and markedly up-regulated in MPC, which might reflect the biological essence of poorer differentiation or dedifferentiation of MPC, and this might have a role in the acquisition and increase of invasiveness and consequent more malignant nature of MPC.
authors: Department of Thoracic Surgery, Kitasato University School of Medicine, Sagamihara, Japan; Department of Pathology, Kitasato University School of Medicine, Sagamihara, Japan.
authors: Hiroyasu
authors: H
authors: Nakashima
authors: Shi-Xu
authors: SX
authors: Jiang
authors: Yuichi
authors: Y
authors: Sato
authors: Keika
authors: K
authors: Hoshi
authors: Toshihide
authors: T
authors: Matsumoto
authors: Ryo
authors: R
authors: Nagashio
authors: Makoto
authors: M
authors: Kobayashi
authors: Yukiko
authors: Y
authors: Matsuo
authors: Kazu
authors: K
authors: Shiomi
authors: Kazushige
authors: K
authors: Hayakawa
authors: Makoto
authors: M
authors: Saegusa
authors: Yukitoshi
authors: Y
authors: Satoh
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D014746:Vimentin / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D014746:Vimentin
keywords: 2-dimensional gel electrophoresis; immunohistochemistry; lung adenocarcinoma; micropapillary adenocarcinoma; proteomics; vimentin