title: Identification of polymorphism in exons 7 and 12 of lactoferrin gene and its association with incidence of clinical mastitis in Murrah buffalo.
abstract: Lactoferrin gene is one of the important candidate genes for mastitis resistance. The gene is located on chromosome BTA 22 and consists of 17 exons spanning over 34.5 kb of genomic DNA. The present study was undertaken with the objectives to identify allelic variants in exons 7 and 12 of lactoferrin gene and to analyze association between its genetic variants and incidence of clinical mastitis in Murrah buffalo. The amplification of exons 7 and 12 of lactoferrin gene yielded amplicons of 232- and 461-bp sizes. PCR-restriction fragment length polymorphism (RFLP) analysis of 232-bp amplicon using BccI restriction enzyme revealed three genotypes (AA, AB, and BB) with frequencies of 0.62, 0.22, and 0.16, respectively. The frequencies of two alleles, A and B, were estimated as 0.73 and 0.27. Hpy188I-RFLP for 461-bp amplicon revealed polymorphism with three genotypes, CC, CD, and DD, with respective frequencies of 0.06, 0.39, and 0.56, whereas frequencies for C and D alleles were 0.25 and 0.75. The chi-square (χ(2)) analysis revealed a significant association between incidence of clinical mastitis and genetic variants of exon 7, and animals of AA genotype of exon 7 were found to be least susceptible to mastitis. The findings indicate potential scope for incorporation of lactoferrin gene in selection and breeding of Murrah buffaloes for improved genetic resistance to mastitis.
authors: Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, Haryana, 132001, India, krishanender25@gmail.com.
authors: Krishanender
authors: K
authors: Dinesh
authors: Archana
authors: A
authors: Verma
authors: Ishwar
authors: I
authors: Das Gupta
authors: Yash Pal
authors: YP
authors: Thakur
authors: Nishant
authors: N
authors: Verma
authors: Ashwani
authors: A
authors: Arya
mesh_terms: D000818:Animals; D002020:Buffaloes; D003612:Dairying; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D015994:Incidence; D007194:India / Q000453:epidemiology; D007781:Lactoferrin / Q000235:genetics*; D008413:Mastitis / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D007781:Lactoferrin