title: Colorimetric peroxidase mimetic assay for uranyl detection in sea water.
abstract: Uranyl (UO2(2+)) is a form of uranium in aqueous solution that represents the greatest risk to human health because of its bioavailability. Different sensing techniques have been used with very sensitive detection limits especially the recently reported uranyl-specific DNAzymes systems. However, to the best of our knowledge, few efficient detection methods have been reported for uranyl sensing in seawater. Herein, gold nanoclusters (AuNCs) are employed in an efficient spectroscopic method to detect uranyl ion (UO2(2+)) with a detection limit of 1.86 μM. In the absence of UO2(2+), the BSA-stabilized AuNCs (BSA-AuNCs) showed an intrinsic peroxidase-like activity. In the presence of UO2(2+), this activity can be efficiently restrained. The preliminary quenching mechanism and selectivity of UO2(2+) was also investigated and compared with other ions. This design strategy could be useful in understanding the binding affinity of protein-stabilized AuNCs to UO2(2+) and consequently prompt the recycling of UO2(2+) from seawater.
authors: Controlled Release and Delivery Lab, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology , Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors: Dingyuan
authors: D
authors: Zhang
authors: Zhuo
authors: Z
authors: Chen
authors: Haneen
authors: H
authors: Omar
authors: Lin
authors: L
authors: Deng
authors: Niveen M
authors: NM
authors: Khashab
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D003124:Colorimetry; D006046:Gold / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D009942:Organometallic Compounds / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism; D012623:Seawater / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014501:Uranium / Q000032:analysis*
chemical_list: D007477:Ions; D008670:Metals; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; C005460:uranyl acetate; D014501:Uranium; D006046:Gold; D009195:Peroxidase
keywords: gold nanoclusters; peroxidase mimetic; proteins; seawater; sensors; uranyl