title: Evaluation of the efficacy of akacid plus® fogging in eradicating causative microorganism in nosocomial infections.
abstract: OBJECTIVE
  The novel polymeric guanidine Akacid Plus® is a member of the cationic family of disinfectants. The aim of the present study was to evaluate the activity of Akacid Plus® against bacteria which cause nosocomial infections and remain viable after contaminating the environment and determine the effects of organic materials to the activity.
  
  
  METHODS
  Closed room and control room were created for experimental disinfection. Bacterial suspensions of 0.5 McFarland were prepared from methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii and vancomycine-resistant Enterococcus faecium (VRE) strains. A 0.1 mL of each suspension was applied on the chipboard (25 cm(2)) and tile (25 cm(2)) test surfaces without albumin and with 2% albumin to simulate organic dirt, and the test surfaces were placed in the test and control rooms after drying. Before testing, cotton swab premoistened with serum physiologic was used to obtain samples from various surfaces in the environment and the samples were transferred onto 5% sheep blood agar for incubation at 37°C. Akacid Plus® solution at a concentration of 0.5% was nebulized with an aerosol applicator (Prowi-06, Germany) for 45 minutes. After a 2-hour waiting period, 1 mL neutralizing broth (Dey-Engley Neutralizing Broth, Fluka) was transferred on the test surfaces, and samples were collected with a swab from the test surfaces and various surfaces in the testing room and inoculated on 5% sheep blood agar for incubation at 37oC for 24 hours. At the end of the incubation period, number of colonies were evaluated on the control and test plates.
  
  
  RESULTS
  Although coagulase-negative staphylococci, Bacillus spp., and fungi were grown in cultured samples obtained from the environment of experimental laboratory, no growth was observed in the test plates after room disinfection with Akacid Plus®. After room disinfection, MRSA and A. baumannii were not detectable in the cultured media prepared from the test surfaces with or without albumin. The bacterial count for vancomyine-resistant E. faecium was reduced from 10(7) to 5×10(2) on surfaces without albumin and from 10(7) to 2.5×10(3) on surfaces with albumin. All test plates prepared from the surfaces in the control room showed abundant growth of the microorganism.
  
  
  CONCLUSIONS
  The nebulization of Akacid plus® solution at a concentration of 0.5% proved to be an efficient means of disinfection for the removal of pathogenic microorganisms that cause hospital outbreaks and use of isolation measures.
authors: Department of Microbiology, Samsun Maternity and Children Hospital Samsun.
authors: Nevzat
authors: N
authors: Unal
authors: Department of Medical Microbiology, Ondokuz Mayıs University Faculty of Medicine Samsun.
authors: Keramettin
authors: K
authors: Yanik
authors: Department of Medical Microbiology, Ondokuz Mayıs University Faculty of Medicine Samsun.
authors: Adil
authors: A
authors: Karadag
authors: Department of Medical Microbiology, Ondokuz Mayıs University Faculty of Medicine Samsun.
authors: Hakan
authors: H
authors: Odabaşı
authors: Department of Infectious Diseases and Clinical Microbiology, Ondokuz Mayıs University Faculty of Medicine Samsun.
authors: Saban
authors: S
authors: Esen
authors: Department of Medical Microbiology, İstanbul University Cerrahpaşa Faculty of Medicine İstanbul.
authors: Murat
authors: M
authors: Günaydin
keywords: A. baumanni; Akacid plus®; MRSA; VRE