title: Increased New lncRNA-mRNA Gene Pair Levels in Human Cumulus Cells Correlate With Oocyte Maturation and Embryo Development.
abstract: The close relationship between cumulus cells and oocyte indicates that the analysis of cumulus gene expression is a potential noninvasive method to aid embryo selection and in vitro fertilization outcome. Long noncoding RNAs (LncRNAs) could regulate essential pathways that contribute to human oocyte maturation, fertilization, and embryo development, which indicates that lncRNA would be valuable biomarkers. In our previous study, AK124742 is a newly detected lncRNA that was identified as being natural antisense to PSMD6, but its role in oocyte and embryo development is still not elucidated and needs to be investigated. Here, the expression of AK124742 and PSMD6 was measured in 40 pairs of cumulus cells from oocytes that result in high-quality embryos (HCCs) and from oocytes that result in poor-quality embryos (PCCs) by real-time quantitative reverse transcriptase polymerase chain reaction. The predictive value of AK124742 and PSMD6 was evaluated using a receiver-operating characteristic (ROC) curve. Notably, elevated expression levels of AK124742 and PSMD6 were observed in HCCs compared to PCCs (72.5% and 62.5%, respectively; P < .01). Expression of AK124742 was potentially positively associated with the PSMD6 levels. The relative expression levels of AK124742 and PSMD6 in the pregnancy group were significantly higher than those in the nonpregnancy group (P < .01).The area under the ROC curve of AK124742 was 0.78 (95% confidence interval: 0.64-0.93). In conclusion, AK124742 and PSMD6 as a new lncRNA-messenger RNA gene pair in human cumulus cells may be considered as potential biomarkers to aid embryo selection.
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Juan
authors: J
authors: Li
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China caoyunxia6@126.com.
authors: Yunxia
authors: Y
authors: Cao
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Xiaofeng
authors: X
authors: Xu
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Huifen
authors: H
authors: Xiang
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Zhiguo
authors: Z
authors: Zhang
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Beili
authors: B
authors: Chen
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Yan
authors: Y
authors: Hao
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Zhaolian
authors: Z
authors: Wei
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Ping
authors: P
authors: Zhou
authors: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors: Dawei
authors: D
authors: Chen
mesh_terms: D019540:Area Under Curve; D001755:Blastocyst / Q000378:metabolism* / Q000473:pathology; D001757:Blastomeres / Q000378:metabolism; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000378:metabolism*; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000378:metabolism*; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056826:Single Embryo Transfer; D016896:Treatment Outcome
chemical_list: C000605970:AK124742 long non-coding RNA, human; D005819:Genetic Markers; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D046988:Proteasome Endopeptidase Complex
keywords: AK124742; PSMD6; cumulus cells; embryo developmental potential; lncRNA