title: Role of serum miRNAs in the prediction of ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients.
abstract: BACKGROUND
  Polycystic ovarian syndrome (PCOS) causes a significantly increased risk of ovarian hyperstimulation syndrome (OHSS). Here, we focused on the altered expression of serum miRNAs and their predictive value for OHSS in PCOS patients.
  
  
  METHODS
  We used the TaqMan low density array followed by individual quantitative reverse transcription-polymerase chain reaction to identify and validate the expression of serum miRNAs in PCOS patients likely to develop severe OHSS.
  
  
  RESULTS
  The miR-16 and miR-223 expression levels were significantly reduced in the patients who were likely to develop severe OHSS than in the control subjects who were likely to develop mild or no OHSS. The sensitivity and specificity of the basal LH, basal LH/FSH, and body mass index (BMI) as OHSS predictors were also evaluated. miR-16 was the most efficient for OHSS prediction as it yielded the highest AUC. Logistic binary regression analyses revealed a positive association of miR-223 and BMI.
  
  
  CONCLUSIONS
  Serum miRNAs are differentially expressed in PCOS patients likely to suffer from severe OHSS. We identified and validated two serum miRNAs that have potential for use as novel noninvasive biomarkers to accurately predict OHSS before controlled ovarian hyperstimulation (COH) for PCOS patients.
authors: State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Chun
authors: C
authors: Zhao
authors: Xiaoguang
authors: X
authors: Liu
authors: Zhonghua
authors: Z
authors: Shi
authors: Jing
authors: J
authors: Zhang
authors: Junqiang
authors: J
authors: Zhang
authors: Xuemei
authors: X
authors: Jia
authors: Xiufeng
authors: X
authors: Ling
mesh_terms: D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood*; D016471:Ovarian Hyperstimulation Syndrome / Q000097:blood* / Q000150:complications / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000150:complications / Q000473:pathology
chemical_list: D035683:MicroRNAs; C505362:MIRN16 microRNA, human; C525067:MIR223, human