title: Improved tagmentation-based whole-genome bisulfite sequencing for input DNA from less than 100 mammalian cells.
abstract: OBJECTIVE
  To develop a whole-genome methylation sequencing method that fulfills the needs for studies using ultra-low-input DNA.
  
  
  METHODS
  The tagmentation-based whole-genome bisulfite sequencing (T-WGBS) technology is modified, enabling stable library construction with complexity from minimally 0.5 ng of initial genomic DNA, which equals less than 100 mammalian cells.
  
  
  RESULTS
  We thoroughly assessed the performance of this T-WGBS method by sequencing the methylomes of a rice strain and pre-implantation embryos of rhesus monkey and compare to traditional WGBS approach, thereby demonstrating the efficacy of this new approach.
  
  
  CONCLUSIONS
  This new approach is highly attractive for the complete methylome analysis of very few cells, for example, mammalian pre-implantation embryos, or tiny human biopsy specimens.
authors: BGI-Shenzhen, Shenzhen, China.
authors: Hanlin
authors: H
authors: Lu
authors: Zhimei
authors: Z
authors: Yuan
authors: Tao
authors: T
authors: Tan
authors: Junwen
authors: J
authors: Wang
authors: Juyong
authors: J
authors: Zhang
authors: Hui-Juan
authors: HJ
authors: Luo
authors: Yudong
authors: Y
authors: Xia
authors: Weizhi
authors: W
authors: Ji
authors: Fei
authors: F
authors: Gao
mesh_terms: D000818:Animals; D019175:DNA Methylation; D023281:Genomics / Q000379:methods; D008253:Macaca mulatta; D012275:Oryza / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite
keywords: DNA methylation; T-WGBS; Tn5 transposase; WGBS