title: Interaction effects of dietary supplementation of heat-killed Lactobacillus plantarum and β-glucan on growth performance, digestibility and immune response of juvenile red sea bream, Pagrus major.
abstract: Both heat-killed Lactobacillus plantarum (HK-LP) and β-glucan (BG) play important roles in growth performance, feed utilization and health status of fish. Therefore, a feeding trial was conducted to determine the interactive effects of dietary HK-LP and BG on growth performance, digestibility, oxidative status and immune response of red sea bream for 56 days. A significant interaction was found between HK-LP and BG on final body weight, total plasma protein, glucose, serum bactericidal activity (BA), total serum protein, serum alternative complement pathway (ACP) activity, protein and dry matter digestibility coefficients (P < 0.05). In addition, body weight gain, specific growth rate, feed intake, protein efficiency ratio as well as serum lysozyme activity, ACP activity and mucus secretion were significantly affected by either HK-LP or BG (P < 0.05). Further, feeding 0.025% HK-LP combined with 0.1% BG significantly increased serum peroxidase activity compared with the other groups (P < 0.05). However, protein body content, somatic parameters, total bilirubin, blood urea nitrogen, glutamyl oxaloacetic transaminase (GOT), glutamic-pyruvate transaminase (GPT), triglycerides and mucus BA were not significantly altered by supplementations (P > 0.05). Interestingly, fish fed with both HK-LP at (0.025 and 0.1%) in combination with BG at (0 and 0.1%) showed higher oxidative stress resistance. Under the experimental conditions, dietary HK-LP and BG had a significant interaction on enhancing the growth, digestibility and immune responses of red sea bream.
authors: The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, Japan; Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt. Electronic address: mahmouddawood55@gmail.com.
authors: Mahmoud A O
authors: MA
authors: Dawood
authors: Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.
authors: Shunsuke
authors: S
authors: Koshio
authors: Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.
authors: Manabu
authors: M
authors: Ishikawa
authors: Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.
authors: Saichiro
authors: S
authors: Yokoyama
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D004032:Diet / Q000662:veterinary; D004063:Digestion / Q000187:drug effects; D006358:Hot Temperature; D007113:Immunity, Innate / Q000187:drug effects*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D010473:Perciformes / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D047071:beta-Glucans / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans
keywords: Growth; Heat-killed Lactobacillus plantarum; Immunity; Red sea bream; β-glucan