title: Bioaccessibility versus bioavailability of essential (Cu, Fe, Mn, and Zn) and toxic (Pb) elements from phyto hyperaccumulator Pistia stratiotes: potential risk of dietary intake.
abstract: Aquatic weeds are widely used as animal feed in developing countries. However, information about element bioavailability from these plants is lacking. A combination of an in vitro method [physiologically based extraction test (PBET)] and an in vivo feeding trial was used in this study to investigate potential element bioaccessibility and estimated bioavailability of Pistia stratiotes (PS). Cu, Fe, Mn, Zn, and Pb concentrations in PS biomass, artificial gastrointestinal fluids, and rat tissues were determined using atomic absorption spectrometry with electrothermal atomization and inductively coupled plasma-atomic emission spectrometry. PS exhibited elevated Fe, Mn, and Pb levels. The PBET revealed high bioaccessibility of all monitored elements from PS biomass. The results of the in vivo trial were inconsistent with those of the PBET, because animals fed PS exhibited low levels of essential elements in the tissues. The consumption of a PS-supplemented diet significantly decreased total Fe levels and increased the total level of accumulation of Pb in exposed animals. Significantly reduced amounts of essential elements in the intestinal walls indicated a potential disruption in nutrient gastrointestinal absorption in animals fed PS.
authors: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague , Kamýcká 957, Prague 6, 165 21 Czech Republic.
authors: Zuzana
authors: Z
authors: Čadková
authors: Jiřina
authors: J
authors: Száková
authors: Daniela
authors: D
authors: Miholová
authors: Barbora
authors: B
authors: Horáková
authors: Oldřich
authors: O
authors: Kopecký
authors: Daniela
authors: D
authors: Křivská
authors: Iva
authors: I
authors: Langrová
authors: Pavel
authors: P
authors: Tlustoš
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000825:Animal Structures / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D029064:Araceae / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000032:analysis / Q000378:metabolism*; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D007854:Lead / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D008297:Male; D008345:Manganese / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D015032:Zinc / Q000032:analysis / Q000378:metabolism*
chemical_list: D012989:Soil Pollutants; D007854:Lead; D008345:Manganese; D003300:Copper; D007501:Iron; D015032:Zinc
keywords: Pistia stratiotes; biokinetics; feeding trial; microelements; physiologically based extraction test; risk elements