title: Fibronectin changes in eosinophilic meningitis with blood-CSF barrier disruption.
abstract: Fibronectin, which is present at relatively low levels in healthy central nervous systems (CNS), shows increased levels in meningitis. In this study, fibronectin processing was correlated with the increased permeability of the blood-cerebrospinal fluid (CSF) barrier as well as with the formation of eosinophil infiltrates in angiostrongyliasis meningitis. The immunohistochemistry results show matrix metalloproteinase-9 (MMP-9) is localized in the choroid plexus epithelium. Coimmunoprecipitation demonstrated fibronectin strongly binds MMP-9. Furthermore, treatment with the MMP-9 inhibitor GM6001 significantly inhibited fibronectin processing, reduced the blood-CSF barrier permeability, and decreased the eosinophil counts. The decreased fibronectin processing in CSF implies decreased cellular invasion of the subarachnoid space across the blood-CSF barrier. Therefore, increased fibronectin processing may be associated with barrier disruption and participate in the extravasation and migration of eosinophils into the CNS during experimental parasitic infection.
authors: Department of Parasitology, School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
authors: Ling-Yuh
authors: LY
authors: Shyu
authors: Department of Anatomy, School of Medicine, Chung-Shan Medical University, Taichung 402, Taiwan.
authors: Ming-E
authors: ME
authors: Hu
authors: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
authors: Chun-Hui
authors: CH
authors: Chou
authors: Department of Parasitology, School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
authors: Ke-Min
authors: KM
authors: Chen
authors: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
authors: Ping-Sung
authors: PS
authors: Chiu
authors: Department of Parasitology, School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 402, Taiwan. Electronic address: shih@csmu.edu.tw.
authors: Shih-Chan
authors: SC
authors: Lai
mesh_terms: D017185:Angiostrongylus cantonensis; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002831:Choroid Plexus / Q000201:enzymology; D004151:Dipeptides / Q000494:pharmacology; D004802:Eosinophilia / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism* / Q000469:parasitology; D005353:Fibronectins / Q000134:cerebrospinal fluid / Q000276:immunology / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D008581:Meningitis / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010539:Permeability; D011897:Random Allocation; D051381:Rats; D012908:Snails; D013047:Specific Pathogen-Free Organisms; D017206:Strongylida Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D004151:Dipeptides; D005353:Fibronectins; D061965:Matrix Metalloproteinase Inhibitors; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D020780:Matrix Metalloproteinase 9
keywords: Angiostrongylus cantonensis; Evans blue; Fibronectin; Meningitis