title: Renal extraction and acute effects of glucagon-like peptide-1 on central and renal hemodynamics in healthy men.
abstract: The present experiments were performed to elucidate the acute effects of intravenous infusion of glucagon-like peptide (GLP)-1 on central and renal hemodynamics in healthy men. Seven healthy middle-aged men were examined on two different occasions in random order. During a 3-h infusion of either GLP-1 (1.5 pmol·kg⁻¹·min⁻¹) or saline, cardiac output was estimated noninvasively, and intraarterial blood pressure and heart rate were measured continuously. Renal plasma flow, glomerular filtration rate, and uptake/release of hormones and ions were measured by Fick's Principle after catheterization of a renal vein. Subjects remained supine during the experiments. During GLP-1 infusion, both systolic blood pressure and arterial pulse pressure increased by 5±1 mmHg (P=0.015 and P=0.002, respectively). Heart rate increased by 5±1 beats/min (P=0.005), and cardiac output increased by 18% (P=0.016). Renal plasma flow and glomerular filtration rate as well as the clearance of Na⁺ and Li⁺ were not affected by GLP-1. However, plasma renin activity decreased (P=0.037), whereas plasma levels of atrial natriuretic peptide were unaffected. Renal extraction of intact GLP-1 was 43% (P<0.001), whereas 60% of the primary metabolite GLP-1 9-36amide was extracted (P=0.017). In humans, an acute intravenous administration of GLP-1 leads to increased cardiac output due to a simultaneous increase in stroke volume and heart rate, whereas no effect on renal hemodynamics could be demonstrated despite significant extraction of both the intact hormone and its primary metabolite.
authors: Department of Clinical Physiology and Nuclear Medicine, Bispebjerg University Hospital, Copenhagen, Denmark; aliasmar@sund.ku.dk.
authors: Ali
authors: A
authors: Asmar
authors: Department of Clinical Physiology and Nuclear Medicine, Bispebjerg University Hospital, Copenhagen, Denmark;
authors: Lene
authors: L
authors: Simonsen
authors: Department of Clinical Physiology and Nuclear Medicine, Bispebjerg University Hospital, Copenhagen, Denmark;
authors: Meena
authors: M
authors: Asmar
authors: Department of Endocrinology, Hvidovre University Hospital, Copenhagen, Denmark;
authors: Sten
authors: S
authors: Madsbad
authors: NNF Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark;
authors: Jens J
authors: JJ
authors: Holst
authors: Department of Diagnostics, Clinical Physiology and Nuclear Medicine, Glostrup University Hospital, Copenhagen, Denmark; and.
authors: Erik
authors: E
authors: Frandsen
authors: Institut National de la Santé et de la Recherche Médicale, UMR 1048, Institute of Metabolic and Cardiovascular Diseases, Paul Sabatier University, Toulouse, France.
authors: Cedric
authors: C
authors: Moro
authors: Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark;
authors: Thomas
authors: T
authors: Jonassen
authors: Department of Clinical Physiology and Nuclear Medicine, Bispebjerg University Hospital, Copenhagen, Denmark; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark;
authors: Jens
authors: J
authors: Bülow
mesh_terms: D001775:Blood Circulation; D001794:Blood Pressure; D002302:Cardiac Output; D002404:Catheterization; D005919:Glomerular Filtration Rate; D052216:Glucagon-Like Peptide 1 / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006339:Heart Rate; D006801:Humans; D007262:Infusions, Intravenous; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D010455:Peptides / Q000097:blood / Q000378:metabolism / Q000652:urine; D017534:Radial Artery; D012079:Renal Circulation; D065667:Renal Elimination; D012082:Renal Veins; D012083:Renin / Q000097:blood; D014882:Water-Electrolyte Balance
chemical_list: D010455:Peptides; C114437:glucagon-like peptide-1 (9-36)-amide; D052216:Glucagon-Like Peptide 1; D012083:Renin
keywords: blood pressure; cardiac output; glucagon-like peptide-1; heart rate; renal plasma flow