title: Developing an effective therapeutic by delivery of synthetic microRNA-520e in lung cancer treatment.
abstract: MicroRNAs (miRNAs) can function as tumor suppressors and might provide an efficient strategy for annihilating cancer. Specific miRNAs can be reintroduced into tumor cells to complement the loss of tumor suppression activities. The "miRNA replacement therapy" is based on the concept that the reintroduction of miRNAs depleted in cancer cells reactivates cellular pathways that lead to therapeutic responses. Here, we report the development of miRNA delivery formulation using synthesized miR-520e. This formulation proved to be effective either locally or systematically. MiR-520e accumulation becomes evident in tumor cells and then exerts anti-proliferative function. Meanwhile, intravenous delivery of formulated miR-520e does not induce any deregulation in cytokine levels and liver enzymes. Taken together, our results shed new lights on the concept that systematic delivery of synthetic mimics for tumor suppressor miR-520e and provide potential implications for miRNA therapy in clinic.
authors: Department of Respiratory Medicine, Qilu Hospital, Shandong University, Shandong, China.
authors: Dedong
authors: D
authors: Ma
authors: Department of Anaesthesiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Shandong, China.
authors: Hongxiu
authors: H
authors: Lu
authors: Department of Respiratory Medicine, Qilu Hospital, Shandong University, Shandong, China.
authors: Yiqing
authors: Y
authors: Qu
authors: Department of Geratology, Qilu Hospital, Shandong University, Shandong, China.
authors: Weijiang
authors: W
authors: Fu
authors: Department of Ultrasound, Qilu Hospital, Shandong University, Shandong, China. Electronic address: manumazhe@163.com.
authors: Zhe
authors: Z
authors: Ma
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016207:Cytokines / Q000097:blood; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D007249:Inflammation / Q000473:pathology; D007275:Injections, Intravenous; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
chemical_list: D016207:Cytokines; C575048:MIRN520 microRNA, human; D035683:MicroRNAs
keywords: Lung cancer treatment; Systematic delivery; microRNA-520e