title: Altered expression of platelet factor 4 and basic fibroblast growth factor correlates with the inhibition of tumor growth in mice.
abstract: Herein, we describe the effects of Taxol on endothelioma cell growth and migration in vitro and on vascular tumor growth in vivo. The effects of Taxol on endothelioma cell growth were determined using the crystal violet assay, while cell migration was measured using the xCELLIgence Real-Time Cell Analysis system. To study the effects of Taxol on tumor growth, mice were inoculated with endothelioma cells to induce vascular tumor development and were treated with the drug. At termination, tissue samples from Taxol-treated and control mice were stained with hematoxylin and eosin for histological examination, while blood samples were collected for hematological analysis, as well as for the analysis of the expression of angiogenic markers. In vitro, Taxol inhibited cell growth and migration. The drug also inhibited vascular tumor growth in mice, and this correlated with a recovery of mice from thrombocytopenia. Array analysis of blood samples from mice revealed that there was an increase in the expression of platelet factor 4 and a suppression of the proangiogenic molecule basic fibroblast growth factor in Taxol-treated animals. Our findings suggest that Taxol may have potential in the treatment of vascular tumors.
authors: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Tshwane, South Africa. Electronic address: peace.mabeta@up.ac.za.
authors: Peace
authors: P
authors: Mabeta
authors: Immunology, Faculty of Health Sciences, University of Pretoria, Tshwane, South Africa.
authors: Michael S
authors: MS
authors: Pepper
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000097:blood / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology; D010978:Platelet Factor 4 / Q000378:metabolism*
chemical_list: D000906:Antibodies; D016222:Fibroblast Growth Factor 2; D010978:Platelet Factor 4; D017239:Paclitaxel
keywords: Angiogenesis; Cell migration; PF4; Taxol; Vascular tumor; bFGF