title: Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing.
abstract: Identifying sources of Mycobacterium bovis transmission would be essential for establishing effective control programs of bovine tuberculosis (TB), a major zoonosis threatening human health worldwide. As an effort to determine the extent of M. bovis transmission among dairy and beef cattle and deer populations, a mycobacterial interspersed repetitive units (MIRU)-variable-number tandem repeats (VNTR) typing method was employed for analysis of 131 M. bovis isolates from 59 Holstein dairy cattle, 39 Korean beef cattle, and 33 deer. Of 31 MIRU-VNTR markers, 15 showed allelic diversity. The most discriminatory locus for M. bovis isolates was VNTR 3336 (h=0.59) followed by QUB 26, MIRU 31, VNTR 2401, and VNTR 3171 which showed high discriminatory power (h=0.43). The combined VNTR loci had an allelic diversity of 0.83. On the basis of the VNTR profiles of 30 VNTR loci, 24 genotypes were identified, and two genotypes were highly prevalent among all M. bovis isolates (33.6% and 19.1%, respectively), thus indicating that more than 50% of the isolates shared common molecular characteristics. Six additional genotypes were common in 2 of the 3 animal species, suggesting a wide interspecies transmission of M. bovis. This study thus demonstrates that MIRU-VNTR typing is useful in differentiation of M. bovis isolates and that M. bovis transmission occurs frequently among farmed animal species, highlighting the importance of bovine TB control programs in different animal species which are often raised in the same villages.
authors: Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors: Sungmo
authors: S
authors: Je
authors: Bacteriology and Parasitology Division, Animal and Plant Quarantine Agency, Anyang 430-757, Gyeonggi-do, Republic of Korea.
authors: Bok Kyung
authors: BK
authors: Ku
authors: Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors: Bo-Young
authors: BY
authors: Jeon
authors: Bacteriology and Parasitology Division, Animal and Plant Quarantine Agency, Anyang 430-757, Gyeonggi-do, Republic of Korea.
authors: Jae-Myoung
authors: JM
authors: Kim
authors: Bacteriology and Parasitology Division, Animal and Plant Quarantine Agency, Anyang 430-757, Gyeonggi-do, Republic of Korea.
authors: Suk-Chan
authors: SC
authors: Jung
authors: Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea. Electronic address: raycho@yuhs.ac.
authors: Sang-Nae
authors: SN
authors: Cho
mesh_terms: D000483:Alleles; D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000635:transmission*; D003612:Dairying; D003670:Deer / Q000382:microbiology*; D005260:Female; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea / Q000453:epidemiology; D014376:Tuberculosis / Q000382:microbiology / Q000635:transmission*; D014380:Tuberculosis, Bovine / Q000382:microbiology / Q000635:transmission*
keywords: Bovine tuberculosis; Korea; Mycobacterium bovis; VNTR typing