title: Identification and analysis of isothiocyanates and new acylated anthocyanins in the juice of Raphanus sativus cv. Sango sprouts.
abstract: The freeze-dried sprouts' juice of Raphanus sativus (L.) cv. Sango was prepared and analysed for the first time. HPLC analysis of total isothiocyanates, after protein displacement, resulted in 77.8 ± 3.0 μmol/g of dry juice while GC-MS analysis of hexane and acetone extracts showed E- and Z-raphasatin (8.9 and 0.11 μmol/g, respectively) and sulforaphene (11.7 μmol/g), summing up to 20.7 ± 1.7 μmol/g of free isothiocyanates. Sprouts' juice contained an unprecedented wealth of anthocyanins and a new fractionation methodology allowed us to isolate 34 mg/g of acylated anthocyanins (28.3 ± 1.9 μmol/g), belonging selectively to the cyanidin family. Analysis was performed by HPLC-PDA-ESI-MS(n) and extended to deacylated anthocyanins and aglycones, obtained, respectively, by alkaline and acid hydrolysis. This study identified 70 anthocyanins, 19 of which have never been described before and 32 of which are reported here in R. sativus for the first time. Sango radish sprouts are exceptional dietary sources of heath-promoting micronutrients.
authors: R&D Division, BeC S.r.l., Via C. Monteverdi 49, 47122 Forlì, Italy.
authors: Riccardo
authors: R
authors: Matera
authors: R&D Division, BeC S.r.l., Via C. Monteverdi 49, 47122 Forlì, Italy.
authors: Simone
authors: S
authors: Gabbanini
authors: Industrial Crop Research Centre, Agricultural Research Council, (CRA-CIN), Via di Corticella, 133, 40128 Bologna, Italy.
authors: Gina Rosalinda
authors: GR
authors: De Nicola
authors: Industrial Crop Research Centre, Agricultural Research Council, (CRA-CIN), Via di Corticella, 133, 40128 Bologna, Italy. Electronic address: r.iori@isci.it.
authors: Renato
authors: R
authors: Iori
authors: Department of Organic Chemistry "A. Mangini", University of Bologna, Via S. Giacomo 11, 40126 Bologna, Italy.
authors: Gianna
authors: G
authors: Petrillo
authors: Department of Organic Chemistry "A. Mangini", University of Bologna, Via S. Giacomo 11, 40126 Bologna, Italy. Electronic address: luca.valgimigli@unibo.it.
authors: Luca
authors: L
authors: Valgimigli
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D017879:Isothiocyanates / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D031224:Raphanus / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000872:Anthocyanins; D017879:Isothiocyanates; D010936:Plant Extracts
keywords: Anthocyanin; Antioxidants; Isothiocyanate; Mass spectrometry; Radish sprouts; Sango