title: The role of thionins in rice defence against root pathogens.
abstract: Thionins are antimicrobial peptides that are involved in plant defence. Here, we present an in-depth analysis of the role of rice thionin genes in defence responses against two root pathogens: the root-knot nematode Meloidogyne graminicola and the oomycete Pythium graminicola. The expression of rice thionin genes was observed to be differentially regulated by defence-related hormones, whereas all analysed genes were consistently down-regulated in M. graminicola-induced galls, at least until 7 days post-inoculation (dpi). Transgenic lines of Oryza sativa cv. Nipponbare overproducing OsTHI7 revealed decreased susceptibility to M. graminicola infection and P. graminicola colonization. Taken together, these results demonstrate the role of rice thionin genes in defence against two of the most damaging root pathogens attacking rice.
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.|Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
authors: Hongli
authors: H
authors: Ji
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Godelieve
authors: G
authors: Gheysen
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Chhana
authors: C
authors: Ullah
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.|Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Ruben
authors: R
authors: Verbeek
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Chenjing
authors: C
authors: Shang
authors: Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: David
authors: D
authors: De Vleesschauwer
authors: Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Monica
authors: M
authors: Höfte
authors: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors: Tina
authors: T
authors: Kyndt
mesh_terms: D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018517:Plant Roots / Q000276:immunology* / Q000382:microbiology; D054809:Thionins / Q000502:physiology*
chemical_list: D054809:Thionins
keywords: Meloidogyne graminicola; Pythium graminicola; defence; overexpression; qRT-PCR; rice thionins