title: Anaerobic co-digestion of biodiesel waste glycerin with municipal wastewater sludge: microbial community structure dynamics and reactor performance.
abstract: Two 10 L completely mixed reactors operating at 37°C and 20 days SRT were used to evaluate the relationships between reactor performance and microbial community dynamics during anaerobic co-digestion of biodiesel waste glycerin (BWG) with municipal wastewater sludge (MWS). The addition of up to 1.35% (v/v) BWG to reactor feeds yielded increased VS and COD removal together with enhanced the biogas production and methane yield. This represented 50% of the MWS feed COD. Pyrosequencing analysis showed Methanosaeta (acetoclastic) and Methanomicrobium (hydrogenotrophic) to be the methanogenic genera present in greatest diversity during stable reactor operation. Methanosaeta sequences predominated at the lowest BWG loading while those of Methanomicrobium were present in greatest abundance at the higher BWG loadings. Genus Candidatus cloacamonas was present in the greatest number of bacterial sequences at all loadings. Alkalinity, pH, biogas production and methane yield declined and VFA concentrations (especially propionate) increased during the highest BWG loading.
authors: Department of Civil and Environmental Engineering, University of Alberta, 9105-116 St, T6G 2W2 Edmonton, Alberta, Canada. Electronic address: razaviar@ualberta.ca.
authors: Vahid
authors: V
authors: Razaviarani
authors: Department of Civil and Environmental Engineering, University of Alberta, 9105-116 St, T6G 2W2 Edmonton, Alberta, Canada.
authors: Ian D
authors: ID
authors: Buchanan
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D008697:Methane / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics; D012336:RNA, Ribosomal, 16S; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D008697:Methane; D005990:Glycerol
keywords: Biodiesel waste glycerin; Mesophilic anaerobic co-digestion; Microbial dynamics; Municipal wastewater sludge; Reactor performance