title: Characterization of bacterial isolates from rubber dump site and their use in biodegradation of isoprene in batch and continuous bioreactors.
abstract: Bacterial isolates from contaminated soil of a waste rubber dumping site were isolated and characterized using biochemical and molecular approaches. Isoprene degradation kinetics in batch mode (isoprene concentration: 100-1000 ppm) revealed the degradation efficiency of isolates as: Pseudomonas sp. (83%)>Alcaligenes sp. (70%)>Klebsiella sp. (68.5%). The most efficient isolate Pseudomonas sp. was finally inoculated in a specifically designed bioreactor system comprising a bioscrubber and a biofilter packed with polyurethane foam connected in series. The bioscrubber and biofilter units when operated in a series showed more than 90% removal efficiency up to the inlet loading rate (IL) of 371.1g/m(3)/h. Maximum elimination capacity (EC) of biofilter was found to be an order of magnitude greater than that for bioscrubber. Oxidative cleavage of the double bond of isoprene has been revealed through IR spectra of the leachate.
authors: Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors: Navnita
authors: N
authors: Srivastva
authors: Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors: Awadhesh Kumar
authors: AK
authors: Shukla
authors: Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
authors: Ram Sharan
authors: RS
authors: Singh
authors: Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
authors: Siddh Nath
authors: SN
authors: Upadhyay
authors: Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: skdubey@bhu.ac.in.
authors: Suresh Kumar
authors: SK
authors: Dubey
mesh_terms: D000421:Alcaligenes / Q000145:classification / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002070:Butadienes / Q000737:chemistry*; D005374:Filtration; D045782:Hemiterpenes / Q000737:chemistry*; D007700:Kinetics; D007709:Klebsiella / Q000145:classification / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D010420:Pentanes / Q000737:chemistry*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012408:Rubber; D012989:Soil Pollutants / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D064091:Waste Disposal Facilities
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C005059:isoprene; D012408:Rubber; D010100:Oxygen
keywords: Bacterial isolates; Biodegradation; Biofilter; Bioscrubber; Isoprene