title: Optogenetic induction of contractile ability in immature C2C12 myotubes.
abstract: Myoblasts can be differentiated into multinucleated myotubes, which provide a well-established and reproducible muscle cell model for skeletal myogenesis in vitro. However, under conventional differentiation conditions, each myotube rarely exhibits robust contraction as well as sarcomere arrangement. Here, we applied trains of optical stimulation (OS) to C2C12 myotubes, which were genetically engineered to express a channelrhodopsin variant, channelrhodopsin-green receiver (ChRGR), to investigate whether membrane depolarization facilitates the maturation of myotubes. We found that light pulses induced membrane depolarization and evoked action potentials in ChRGR-expressing myotubes. Regular alignments of sarcomeric proteins were patterned periodically after OS training. In contrast, untrained control myotubes rarely exhibited the striated patterns. OS-trained and untrained myotubes also differed in terms of their resting potential. OS training significantly increased the number of contractile myotubes. Treatment with nifedipine during OS training significantly decreased the fraction of contractile myotubes, whereas tetrodotoxin was less effective. These results suggest that oscillations of membrane potential and intracellular Ca(2+) accompanied by OS promoted sarcomere assembly and the development of contractility during the myogenic process. These results also suggest that optogenetic techniques could be used to manipulate the activity-dependent process during myogenic development.
authors: 1] Department of Mechanical Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan [2] Japan Science and Technology Agency (JST), Core Research of Evolutional Science &Technology (CREST), 5 Sanbancho, Chiyoda-ku 102-0075, Japan.
authors: Toshifumi
authors: T
authors: Asano
authors: 1] Japan Science and Technology Agency (JST), Core Research of Evolutional Science &Technology (CREST), 5 Sanbancho, Chiyoda-ku 102-0075, Japan [2] Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
authors: Toru
authors: T
authors: Ishizuka
authors: 1] Department of Mechanical Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan [2] The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita 565-0871, Japan.
authors: Keisuke
authors: K
authors: Morishima
authors: 1] Japan Science and Technology Agency (JST), Core Research of Evolutional Science &Technology (CREST), 5 Sanbancho, Chiyoda-ku 102-0075, Japan [2] Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
authors: Hiromu
authors: H
authors: Yawo
mesh_terms: D000200:Action Potentials; D000818:Animals; D020013:Calcium Signaling; D002460:Cell Line; D015870:Gene Expression; D008027:Light; D051379:Mice; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D032446:Myoblasts / Q000502:physiology; D062308:Optogenetics / Q000379:methods*; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism; D012518:Sarcomeres
chemical_list: D012243:Rhodopsin