title: Bu Shen Huo Xue decoction restores endometrial leukemia-inhibitory factor but not Angiopoietin-2 expression, and improves uterine receptivity in the controlled ovarian stimulation rat model.
abstract: Leukemia-inhibitory factor (LIF) and Angiopoietin-2 (Ang-2) are important factors in fertility. In the present study, it was investigated whether Bu Shen Huo Xue Decoction (BSHXD) prevents controlled ovarian hyperstimulation (COH) treatment-induced changes in endometrial LIF and Ang-2 expression and whether it has an effect on the number of implantation sites and live births in rats. Uteri were collected on day (D) 3, 4 and 5 of pregnancy, and LIF and Ang-2 protein and mRNA expression were detected using western blot analysis and quantitative polymerase chain reaction. On pregnancy D10, the average number of implantation sites was observed. The number of live births from each group was recorded. The results indicated that BSHXD treatment markedly increased the number of live births by restoring endometrial LIF expression and the implantation capacity in the COH rat model. In addition, no association was identified between LIF and Ang-2 expression. Therefore, this suggests that BSHXD may be useful for female reproduction.
authors: Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
authors: Xin
authors: X
authors: Gong
authors: Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
authors: Jiaoying
authors: J
authors: Lou
authors: Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
authors: Qiudan
authors: Q
authors: Lu
authors: Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
authors: Haitao
authors: H
authors: Huang
authors: Reproductive Endocrinology Center, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
authors: Zhe
authors: Z
authors: Jin
keywords: angiogenesis; angiopoietin-2; chinese herbs; implantation; leukemia-inhibitory factor