title: A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology.
abstract: This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules - particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.
authors: a National Centre for Macromolecular Hydrodynamics, Sutton Bonington Campus, University of Nottingham , Sutton Bonington , LE12 5RD , UK.
authors: Richard B
authors: RB
authors: Gillis
authors: Arthur J
authors: AJ
authors: Rowe
authors: Gary G
authors: GG
authors: Adams
authors: Stephen E
authors: SE
authors: Harding
mesh_terms: D003198:Computer Simulation; D057446:Hydrodynamics; D046911:Macromolecular Substances / Q000737:chemistry*; D008832:Microchemistry / Q000379:methods*; D008956:Models, Chemical; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D014461:Ultracentrifugation / Q000379:methods*; D014783:Viscosity
chemical_list: D046911:Macromolecular Substances
keywords: SEC-MALS; analytical ultracentrifugation; biopolymer; hydrodynamics; light scattering; polydisperse; viscometry