title: A simple high-performance liquid chromatography for the determination of linezolid in human plasma and saliva.
abstract: Linezolid is an antimicrobial agent for the treatment of multiresistant Gram-positive infections. A practical high-performance liquid chromatography method was developed for the determination of linezolid in human plasma and saliva. Linezolid and an internal standard (o-ethoxybenzamide) were extracted from plasma and saliva with ethyl acetate and analyzed on a Capcell Pak C18 MG column with UV detection at 254 nm. The calibration curve was linear through the range 0.5-50 µg/mL using a 200 μL sample volume. The intra- and interday precisions were all <6.44% for plasma and 5.60% for saliva. The accuracies ranged from 98.8 to 110% for both matrices. The mean recoveries of linezolid were 80.8% for plasma and 79.0% for saliva. This method was used to determine the plasma and saliva concentrations of linezolid in healthy volunteers who were orally administered a 600 mg dose of linezolid. Our liquid-liquid extraction procedure is easy and requires a small volume of plasma or saliva (200 μL). This small volume can be advantageous in clinical pharmacokinetic studies, especially if children participate.
authors: Faculty of Pharmaceutical Sciences Fukuoka University, Fukuoka, Japan.
authors: Shuuji
authors: S
authors: Hara
authors: Department of Pharmacy, Fukuoka University Hospital, Fukuoka, Japan.
authors: Masanobu
authors: M
authors: Uchiyama
authors: Faculty of Pharmaceutical Sciences Fukuoka University, Fukuoka, Japan.
authors: Masami
authors: M
authors: Yoshinari
authors: Faculty of Pharmaceutical Sciences Fukuoka University, Fukuoka, Japan.
authors: Taichi
authors: T
authors: Matsumoto
authors: Central Laboratory for Pathology and Morphology, Department of Medicine, Fukuoka University, Fukuoka, Japan.
authors: Shiro
authors: S
authors: Jimi
authors: Division of Medical Oncology, Hematology and Infectious Diseases, Department of Medicine, Fukuoka University, Fukuoka, Japan.
authors: Atsushi
authors: A
authors: Togawa
authors: Division of Medical Oncology, Hematology and Infectious Diseases, Department of Medicine, Fukuoka University, Fukuoka, Japan.
authors: Tohru
authors: T
authors: Takata
authors: Division of Medical Oncology, Hematology and Infectious Diseases, Department of Medicine, Fukuoka University, Fukuoka, Japan.
authors: Yasushi
authors: Y
authors: Takamatsu
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D000069349:Linezolid / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000069349:Linezolid
keywords: HPLC-UV; linezolid; plasma; saliva; therapeutic drug monitoring