title: Poly(vinyl alcohol)-coated silver nanoparticles: activation of neutrophils and nanotoxicology effects in human hepatocarcinoma and mononuclear cells.
abstract: Silver nanoparticles (AgNps) have been described as important for their excellent biocompatibility, biomedical applications. Nevertheless, AgNps can interact with the immune system which is essential to analyze human exposure to assess their potential risk to health and environment. In general, the primary site for accumulation of nanoparticles has been demonstrated to be the liver. Furthermore, the direct activation of neutrophils or oxidative burst by a given nanoparticle is poorly documented. In this paper, we investigated the cell uptake, apoptosis, necrosis, DNA damage in human hepatocarcinoma cells (HepG2), primary normal human peripheral blood mononuclear cells (PBMC) and the direct activation of primary isolated neutrophils through the oxidative burst on exposure to AgNps coated with Polyvinyl-alcohol (PVA). All cell types were incubated in the presence of 1.0 and 50.0 μM of AgNps-PVA for 24h. A significant cyto- and genotoxic-response and the activation of human neutrophils were induced by AgNps-PVA (p<0.05). Our results revealed that AgNps can interact with the normal isolated neutrophils, PBMC and HepG2 cells in vitro, which opens the way for further studies on the toxicological effects of AgNps in the human immune system response and cancer cells.
authors: Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, Av. Trab. São Carlense, 400, CEP13566-590 São Carlos, SP, Brazil. Electronic address: iedammp@hotmail.com.
authors: Iêda Maria Martinez
authors: IM
authors: Paino
authors: Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, Av. Trab. São Carlense, 400, CEP13566-590 São Carlos, SP, Brazil.
authors: Valtencir
authors: V
authors: Zucolotto
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D004249:DNA Damage; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008113:Liver Neoplasms; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009336:Necrosis / Q000139:chemically induced; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D012834:Silver; D011142:Polyvinyl Alcohol
keywords: Cytotoxicity; Genotoxicity; Hepatocarcinoma cells; Oxidative burst; PBMC; Silver nanoparticles