title: Downregulation of tNASP inhibits proliferation through regulating cell cycle-related proteins and inactive ERK/MAPK signal pathway in renal cell carcinoma cells.
abstract: Nuclear auto-antigenic sperm protein (NASP), initially described as a highly auto-immunogenic testis and sperm-specific protein, is a histone chaperone that is proved to present in all dividing cells. NASP has two splice variants: testicular NASP (tNASP) and somatic form of NASP (sNASP). Only cancer, germ, transformed, and embryonic cells have a high level of expression of the tNASP. Up to now, little has been known about tNASP in renal cell carcinoma (RCC). In the present study, the molecular mechanism of tNASP in RCC was explored. The expression level of tNASP in 16 paired human RCC specimens was determined. Downregulation of tNASP by small interfering RNA (siRNA) was transfected in RCC cell lines. The effect of downregulation of tNASP by siRNA on cell colony formation and proliferation was examined by colony formation assay and CCK-8 assay, cell cycle was analyzed by flow cytometry, and the expression of cyclin D1 and P21 were detected by Western blotting. ERK/MAPK signaling was also analyzed. tNASP has a relative high expression level in human RCC tissues. Via upregulation of P21 and downregulation of cyclinD1, silence of tNASP can inhibit cell proliferation, which induces cell cycle arrest. Furthermore, ERK signaling pathway is confirmed to mediate the regulation of cell cycle-related proteins caused by silence of tNASP. Our research demonstrates that knockdown of tNASP effectively inhibits the proliferation and causes G1 phase arrest through ERK/MAPK signal pathway.
authors: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China.
authors: Jianzheng
authors: J
authors: Fang
authors: Hainan
authors: H
authors: Wang
authors: Wei
authors: W
authors: Xi
authors: Gong
authors: G
authors: Cheng
authors: Shangqian
authors: S
authors: Wang
authors: Shifeng
authors: S
authors: Su
authors: Shengli
authors: S
authors: Zhang
authors: Yunfei
authors: Y
authors: Deng
authors: Zhen
authors: Z
authors: Song
authors: Aiming
authors: A
authors: Xu
authors: Bianjiang
authors: B
authors: Liu
authors: Jingyi
authors: J
authors: Cao
authors: Zengjun
authors: Z
authors: Wang
mesh_terms: D001324:Autoantigens / Q000096:biosynthesis* / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000096:biosynthesis; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D054462:p21-Activated Kinases / Q000096:biosynthesis
chemical_list: D001324:Autoantigens; D018797:Cell Cycle Proteins; C489390:NASP protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D019938:Cyclin D1; C515907:PAK2 protein, human; D054462:p21-Activated Kinases