title: Multistep microsatellite mutation in a case of non-exclusion parentage.
abstract: A non-exclusion paternity with multistep mutation in the locus D5S818 was reported. Examination of 39 autosomal short tandem repeats (STR) loci revealed a mismatch of the maternally or paternally transmitted allele in the locus D5S818 in the questioned child. The composition of the alleles of this locus in the mother, the questioned child and the alleged father are 11/13, 7/13 and 13, respectively. The sequence analysis of the regions flanking the locus D5S818 of the mother, the questioned child and the alleged father excluded the possibility of null allele as a cause of the allelic mismatch in the child. The combined paternity index of 39 autosomal STRs is up to 2.461×10(9). Genotyping of sixteen Y-STR loci in the questioned child matched completely with the alleged father. The results prove that the alleged father is the biological father of the questioned child with four-step or six-step microsatellite mutation in the locus D5S818.
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Yu Xuan
authors: YX
authors: Liu
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Wen Qiong
authors: WQ
authors: Zhang
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Yun Shu
authors: YS
authors: Jia
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Lei
authors: L
authors: Zhang
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Feng Lei
authors: FL
authors: Zhou
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Kun
authors: K
authors: Mei
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors: Dai Xin
authors: DX
authors: Huang
authors: Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China; AGCU ScienTech Incorporation, Wuxi 214174, China; Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China. Electronic address: yishaoxia@126.com.
authors: Shao Hua
authors: SH
authors: Yi
mesh_terms: D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation; D010334:Paternity
keywords: D5S818; Multistep mutation; Paternity testing; STR