title: Elevated level of HSPA1L mRNA correlates with graft-versus-host disease.
abstract: Graft-versus-host disease (GVHD) can be a fatal complication of allogeneic stem cell transplantation (allo-HSCT). GVHD can be classified as acute (aGVHD: up to 100 days) or chronic (cGVHD: after 100 days) based on the time-point of disease occurrence. At present there are a limited number of biomarkers available for use in the clinic. Thus, the aim of this research was to evaluate the biomarker potential of the extensively studied Heat Shock Protein 70 family members (HSPA1A/HSPA1B and HSPA1L) at the messenger RNA (mRNA) level in acute and cGVHD patient cohorts. In the skin biopsies, HSPA1L mRNA expression was lower in patients with severe aGVHD (grades II-III) when compared to those with none or low grade aGVHD (grades 0-I) and normal controls. In whole blood, HSPA1L mRNA expression level was significantly (p = 0.008) up-regulated at 28 days post-transplant in cGVHD patients with a significant area under the curve (AUC = 0.773). In addition, HSPA1B expression in whole blood was significantly higher at 3 months post-transplant in both the aGVHD grade II-III (p = 0.012) and cGVHD (p = 0.027) patients. Our initial results in this small cohort show that quantifying HSPA1L mRNA expression in the whole blood of allo-HSCT patients at day 28 post-allo-HSCT may be a useful predictive biomarker for cGVHD.
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: s.atarod@newcastle.ac.uk.
authors: Sadaf
authors: S
authors: Atarod
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: briesta@hotmail.com.
authors: Brie
authors: B
authors: Turner
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: kim.pearce@newcastle.ac.uk.
authors: Kim Frances
authors: KF
authors: Pearce
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: shaheda.ahmed@newcastle.ac.uk.
authors: Shaheda S
authors: SS
authors: Ahmed
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: jean.norden@newcastle.ac.uk.
authors: Jean
authors: J
authors: Norden
authors: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. Electronic address: bogunia@iitd.pan.wroc.pl.
authors: Katarzyna
authors: K
authors: Bogunia-Kubik
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: x.n.wang@newcastle.ac.uk.
authors: Xiao-nong
authors: XN
authors: Wang
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: matthew.collin@newcastle.ac.uk.
authors: Matthew
authors: M
authors: Collin
authors: Haematological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom. Electronic address: anne.dickinson@newcastle.ac.uk.
authors: Anne Mary
authors: AM
authors: Dickinson
mesh_terms: D000208:Acute Disease; D064591:Allografts / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D002908:Chronic Disease; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D006086:Graft vs Host Disease / Q000175:diagnosis*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000276:immunology* / Q000473:pathology; D033581:Stem Cell Transplantation
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human; C543454:HSPA1L protein, human; D012333:RNA, Messenger
keywords: Biomarker; Graft-versus-host disease; Heat Shock Protein