title: High-throughput screening approaches to identify regulators of mammalian autophagy.
abstract: This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.
authors: London Research Institute, Cancer Research UK, Secretory Pathway Laboratory, 44 Lincolns Inn Fields, London, WC2A 3LY, UK.
authors: Justin
authors: J
authors: Joachim
authors: London Research Institute, Cancer Research UK, High-throughput Screening Unit, 44 Lincolns Inn Fields, London, WC2A 3LY, UK.
authors: Ming
authors: M
authors: Jiang
authors: Department of Neurology, The Icahn School of Medicine at Mount Sinai, Hess Center for Science and Medicine, 1470 Madison Avenue, New York, NY 10029, USA.
authors: Nicole C
authors: NC
authors: McKnight
authors: London Research Institute, Cancer Research UK, High-throughput Screening Unit, 44 Lincolns Inn Fields, London, WC2A 3LY, UK.
authors: Michael
authors: M
authors: Howell
authors: London Research Institute, Cancer Research UK, Secretory Pathway Laboratory, 44 Lincolns Inn Fields, London, WC2A 3LY, UK. Electronic address: Sharon.tooze@cancer.org.uk.
authors: Sharon A
authors: SA
authors: Tooze
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008322:Mammals; D010588:Phagosomes / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D054852:Small Molecule Libraries / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D034741:RNA, Small Interfering; D054852:Small Molecule Libraries
keywords: Amino acid starvation; Autophagosome; Autophagy; Drug and compound libraries; GFP-LC3; High-throughput screens; Z-score; p62; siRNA