title: Rapid evaporative ionization mass spectrometry imaging platform for direct mapping from bulk tissue and bacterial growth media.
abstract: Rapid evaporative ionization mass spectrometry (REIMS) technology allows real time intraoperative tissue classification and the characterization and identification of microorganisms. In order to create spectral libraries for training the classification models, reference data need to be acquired in large quantities as classification accuracy generally improves as a function of number of training samples. In this study, we present an automated high-throughput method for collecting REIMS data from heterogeneous organic tissue. The underlying instrumentation consists of a 2D stage with an additional high-precision z-axis actuator that is equipped with an electrosurgical diathermy-based sampling probe. The approach was validated using samples of human liver with metastases and bacterial strains, cultured on solid medium, belonging to the species P. aeruginosa, B. subtilis, and S. aureus. For both sample types, spatially resolved spectral information was obtained that resulted in clearly distinguishable multivariate clustering between the healthy/cancerous liver tissues and between the bacterial species.
authors: Section of Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , SW7 2AZ London, United Kingdom.
authors: Ottmar
authors: O
authors: Golf
authors: Nicole
authors: N
authors: Strittmatter
authors: Tamas
authors: T
authors: Karancsi
authors: Steven D
authors: SD
authors: Pringle
authors: Abigail V M
authors: AV
authors: Speller
authors: Anna
authors: A
authors: Mroz
authors: James M
authors: JM
authors: Kinross
authors: Nima
authors: N
authors: Abbassi-Ghadi
authors: Emrys A
authors: EA
authors: Jones
authors: Zoltan
authors: Z
authors: Takats
mesh_terms: D000230:Adenocarcinoma / Q000556:secondary*; D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000254:growth & development; D015179:Colorectal Neoplasms / Q000473:pathology*; D003470:Culture Media / Q000032:analysis*; D003952:Diagnostic Imaging; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008113:Liver Neoplasms / Q000556:secondary*; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003470:Culture Media