title: Chemical constituents of Hericium erinaceum associated with the inhibitory activity against cellular senescence in human umbilical vascular endothelial cells.
abstract: Hericium erinaceum is an edible and medicinal mushroom widely used in Korea, Japan, and China. On the search for biologically active compounds supporting the medicinal usage, the MeOH extract of the fruiting bodies of H. erinaceum was investigated for its chemical constituents. Six compounds were isolated and identified as hericenone D (1), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), erinacerin B (3), hericenone E (4), hericenone F (5) and isohericerin (6) by comparing their spectroscopic data with previously reported values. The inhibitory effects on adriamycin-induced cellular senescence in human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs) of the isolates (1-6) were studied. Among the isolated compounds, ergosterol peroxide (2) reduced senescence associated β-galactosidase (SA-β-gal) activity increased in HUVECs treated with adriamycin. According to experimental data obtained, the active compound may inspire the development of a new pharmacologically useful substance to be used in the treatment and prevention of age-related diseases.
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Hyung Jun
authors: HJ
authors: Noh
authors: b Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .|c Aging-associated Vascular Disease Research Center, Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .
authors: Hyo Hyun
authors: HH
authors: Yang
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Geum Soog
authors: GS
authors: Kim
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Seung Eun
authors: SE
authors: Lee
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Dae Young
authors: DY
authors: Lee
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Je Hun
authors: JH
authors: Choi
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Seung Yu
authors: SY
authors: Kim
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Eun Suk
authors: ES
authors: Lee
authors: a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
authors: Seung Heon
authors: SH
authors: Ji
authors: d College of Korean Medicine , Gachon University , Seongnam , Korea .
authors: Ki Sung
authors: KS
authors: Kang
authors: e Department of Food Science and Biotechnology, College of BioNano Technology , Gachon University , Seongnam , Korea , and.
authors: Hye-Jin
authors: HJ
authors: Park
authors: b Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .|c Aging-associated Vascular Disease Research Center, Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .
authors: Jae-Ryong
authors: JR
authors: Kim
authors: f Natural Product Research Laboratory, School of Pharmacy , Sungkyunkwan University , Suwon , Korea.
authors: Ki Hyun
authors: KH
authors: Kim
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016922:Cellular Senescence / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004875:Ergosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006574:Heterocyclic Compounds, 2-Ring / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D010168:Palmitates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C503471:5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol; D001578:Benzopyrans; D006574:Heterocyclic Compounds, 2-Ring; D007211:Indoles; D010168:Palmitates; D010636:Phenols; D013729:Terpenes; C505043:erinacerin B; C532217:hericenone D; C532218:hericenone E; D004317:Doxorubicin; D001616:beta-Galactosidase; D004875:Ergosterol
keywords: Ergosterol peroxide; Hericium erinaceum; human dermal fibroblasts; human umbilical vein endothelial cells; senescence associated β-galactosidase activity