title: Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis.
abstract: Triptolide (TPL), a traditional Chinese medicine extract, possesses anti-inflammatory and anti-tumor properties. Though some research results have implicated that Triptolide (TPL) can be utilized in the treatment of leukemia, it remains controversial about the mechanism of TPL-induced leukemic T-lymphocytes apoptosis. In this study, combining Raman spectroscopic data, principal component analysis (PCA) and atomic force microscopy (AFM) imaging, both the biochemical changes and morphological changes during TPL-induced cell apoptosis were presented. In contrast, the corresponding data during Daunorubicin (DNR)-induced cell apoptosis was also exhibited. The obtained results showed that Raman spectral changes during TPL-induced cell apoptosis were greatly different from DNR-induced cell apoptosis in the early stage of apoptosis but revealed the high similarity in the late stage of apoptosis. Moreover, above Raman spectral changes were respectively consistent with the morphological changes of different stages during TPL-induced apoptosis or DNR-induced apoptosis, including membrane shrinkage and blebbing, chromatin condensation and the formation of apoptotic bodies. Importantly, it was found that Raman spectral changes with TPL-induced apoptosis or DNR-induced apoptosis were respectively related with the cell cycle G1 phase arrest or G1 and S phase arrest.
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Daosen
authors: D
authors: Zhang
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Yanyan
authors: Y
authors: Feng
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Qinnan
authors: Q
authors: Zhang
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Xin
authors: X
authors: Su
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Xiaoxu
authors: X
authors: Lu
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors: Shengde
authors: S
authors: Liu
authors: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China. Electronic address: zhongly@scnu.edu.cn.
authors: Liyun
authors: L
authors: Zhong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000473:pathology*; D010616:Phenanthrenes / Q000494:pharmacology*; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology*
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide
keywords: Atomic force microscopy (AFM); Cell apoptosis; Daunorubicin (DNR); Principal component analysis (PCA); Raman spectroscopy; Triptolide (TPL)