title: Function of Isolated Pancreatic Islets From Patients at Onset of Type 1 Diabetes: Insulin Secretion Can Be Restored After Some Days in a Nondiabetogenic Environment In Vitro: Results From the DiViD Study.
abstract: The understanding of the etiology of type 1 diabetes (T1D) remains limited. One objective of the Diabetes Virus Detection (DiViD) study was to collect pancreatic tissue from living subjects shortly after the diagnosis of T1D. Here we report the insulin secretion ability by in vitro glucose perifusion and explore the expression of insulin pathway genes in isolated islets of Langerhans from these patients. Whole-genome RNA sequencing was performed on islets from six DiViD study patients and two organ donors who died at the onset of T1D, and the findings were compared with those from three nondiabetic organ donors. All human transcripts involved in the insulin pathway were present in the islets at the onset of T1D. Glucose-induced insulin secretion was present in some patients at the onset of T1D, and a perfectly normalized biphasic insulin release was obtained after some days in a nondiabetogenic environment in vitro. This indicates that the potential for endogenous insulin production is good, which could be taken advantage of if the disease process was reversed at diagnosis.
authors: Paediatric Department, Oslo University Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway lars.krogvold@gmail.com.
authors: Lars
authors: L
authors: Krogvold
authors: Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
authors: Oskar
authors: O
authors: Skog
authors: Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
authors: Görel
authors: G
authors: Sundström
authors: Intervention Centre and Department of Surgery, Oslo University Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Bjørn
authors: B
authors: Edwin
authors: Division of Cancer Medicine, Surgery and Transplantation, Oslo University Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Trond
authors: T
authors: Buanes
authors: Department of Endocrinology, Oslo University Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Kristian F
authors: KF
authors: Hanssen
authors: Division of Paediatrics, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors: Johnny
authors: J
authors: Ludvigsson
authors: Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
authors: Manfred
authors: M
authors: Grabherr
authors: Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
authors: Olle
authors: O
authors: Korsgren
authors: Paediatric Department, Oslo University Hospital and Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Knut
authors: K
authors: Dahl-Jørgensen
mesh_terms: D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D059865:Endoplasmic Reticulum Stress; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000503:physiopathology*; D008297:Male; D059467:Transcriptome
chemical_list: D007328:Insulin