title: Effect of fermented wheat germ extract with lactobacillus plantarum dy-1 on HT-29 cell proliferation and apoptosis.
abstract: This study aimed to evaluate the anticarcinogenic activities of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 (LFWGE). The anticarcinogenic activities, including antiproliferative effects and the induction of apoptosis, were studied in human HT-29 colon cancer cells. The 2,6-dimethoxybenzoquinone and total phenol contents in LFWGE were determined by HPLC and the Folin-Ciocalteu method. In addition, some functional proteins were separated and purified by gel filtration chromatography. There were 21 proteins identified by LC-MS/MS. The sugars isolated from LFWGE did not possess any anticarcinogenic activity. The results of an MTT assay showed high antiproliferative effects of LFWGE. In addition, LFWGE attenuated the progression from the G0-G1 to the G2-M phase of the cell cycle, and LFWGE-induced cell apoptosis was associated with the activation of caspase-3. LFWGE and its major bioactive ingredients inhibited the proliferation of HT-29 cells via apoptosis and thus may be a potential anticarcinogenic agent.
authors: School of Food and Biological Engineering, Jiangsu University , 301 Xuefu Road, Zhenjiang, 212013, China.
authors: Jia-Yan
authors: JY
authors: Zhang
authors: Xiang
authors: X
authors: Xiao
authors: Ying
authors: Y
authors: Dong
authors: Jing
authors: J
authors: Wu
authors: Fang
authors: F
authors: Yao
authors: Xing-Hua
authors: XH
authors: Zhou
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000503:physiopathology*; D005285:Fermentation; D019073:HT29 Cells; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C113915:Avemar; D010936:Plant Extracts
keywords: 2; 6-dimethoxybenzoquinone; apoptosis; colon cancer; fermented wheat germ extract; protein