title: Expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, HutX, from Vibrio cholerae.
abstract: Vibrio cholerae, the causative agent of cholera, has developed a variety of mechanisms to obtain the limited-availability iron from human hosts. One important method for iron acquisition is through haem-uptake systems. Although the transport of haem has been widely studied, the fate of haem once it enters the cytoplasm remains an open question. Here, preliminary X-ray crystallographic analysis was performed on HutX, a member of the conserved haem-utilization operon from V. cholerae strain N16961. The crystals of HutX were found to belong to the orthorhombic space group C2221, with unit-cell parameters a = 50.1, b = 169.0, c = 81.8 Å. There are two protein molecules in the asymmetric unit, with a corresponding Matthews coefficient VM of 2.06 Å(3) Da(-1) and a solvent content of 40.28%.
authors: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, Shandong 250100, People's Republic of China.
authors: Tiantian
authors: T
authors: Su
authors: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, Shandong 250100, People's Republic of China.
authors: Kaikai
authors: K
authors: Chi
authors: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, Shandong 250100, People's Republic of China.
authors: Kang
authors: K
authors: Wang
authors: Rizhao Center for Disease Prevention and Control, Rizhao Health Bureau, 136 Beijing Road, Rizhao, Shandong 276826, People's Republic of China.
authors: Liming
authors: L
authors: Guo
authors: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, Shandong 250100, People's Republic of China.
authors: Yan
authors: Y
authors: Huang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000378:metabolism*; D006801:Humans; D013057:Spectrum Analysis; D014734:Vibrio cholerae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D006418:Heme
keywords: Vibrio cholerae; haem uptake; iron acquisition