title: Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides.
abstract: Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD(+))-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules.
authors: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors: Musleh M
authors: MM
authors: Muthana
authors: Jingyao
authors: J
authors: Qu
authors: Mengyang
authors: M
authors: Xue
authors: Timofey
authors: T
authors: Klyuchnik
authors: Alex
authors: A
authors: Siu
authors: Yanhong
authors: Y
authors: Li
authors: Lei
authors: L
authors: Zhang
authors: Hai
authors: H
authors: Yu
authors: Lei
authors: L
authors: Li
authors: Peng G
authors: PG
authors: Wang
authors: Xi
authors: X
authors: Chen
mesh_terms: D000244:Adenosine Diphosphate / Q000096:biosynthesis* / Q000737:chemistry; D017360:Arabidopsis / Q000201:enzymology; D001644:Bifidobacterium / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D020719:Glucuronides / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D014574:Uronic Acids / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020719:Glucuronides; D014574:Uronic Acids; D000244:Adenosine Diphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C020591:glucuronokinase