title: Multiple steps control immunity during the intracellular accommodation of rhizobia.
abstract: Medicago truncatula belongs to the legume family and forms symbiotic associations with nitrogen fixing bacteria, the rhizobia. During these interactions, the plants develop root nodules in which bacteria invade the plant cells and fix nitrogen for the benefit of the plant. Despite massive infection, legume nodules do not develop visible defence reactions, suggesting a special immune status of these organs. Some factors influencing rhizobium maintenance within the plant cells have been previously identified, such as the M. truncatula NCR peptides whose toxic effects are reduced by the bacterial protein BacA. In addition, DNF2, SymCRK, and RSD are M. truncatula genes required to avoid rhizobial death within the symbiotic cells. DNF2 and SymCRK are essential to prevent defence-like reactions in nodules after bacteria internalization into the symbiotic cells. Herein, we used a combination of genetics, histology and molecular biology approaches to investigate the relationship between the factors preventing bacterial death in the nodule cells. We show that the RSD gene is also required to repress plant defences in nodules. Upon inoculation with the bacA mutant, defence responses are observed only in the dnf2 mutant and not in the symCRK and rsd mutants. In addition, our data suggest that lack of nitrogen fixation by the bacterial partner triggers bacterial death in nodule cells after bacteroid differentiation. Together our data indicate that, after internalization, at least four independent mechanisms prevent bacterial death in the plant cell. These mechanisms involve successively: DNF2, BacA, SymCRK/RSD and bacterial ability to fix nitrogen.
authors: Institut des Sciences du Végétal, CNRS, Saclay Plant Sciences, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors: Fathi
authors: F
authors: Berrabah
authors: Institut des Sciences du Végétal, CNRS, Saclay Plant Sciences, Avenue de la Terrasse, 91198 Gif sur Yvette, France benjamin.gourion@isv.cnrs-gif.fr ratet@isv.cnrs-gif.fr.
authors: Pascal
authors: P
authors: Ratet
authors: Institut des Sciences du Végétal, CNRS, Saclay Plant Sciences, Avenue de la Terrasse, 91198 Gif sur Yvette, France benjamin.gourion@isv.cnrs-gif.fr ratet@isv.cnrs-gif.fr.
authors: Benjamin
authors: B
authors: Gourion
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D046913:Medicago truncatula / Q000166:cytology / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D010641:Phenotype; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D053204:Root Nodules, Plant / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000502:physiology*; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D009584:Nitrogen
keywords: CRK; DNF2; bacA; innate immunity; nifA; nifH; nitrogen fixation.