title: Anti-inflammatory effect of resveratrol in old mice liver.
abstract: Inflammation is a hallmark of aging. Caloric restriction and resveratrol (RSV) have shown important effects on prevention of oxidative stress and inflammation. Here, we investigate the progression of proinflammatory markers in liver during aging and the effect of RSV on inflammation markers in the liver of old male C57BL/6J mice. Young (2 months), mature (12 months) and old (18 months) mice were fed during 6 months with RSV. Levels of IL-1β, IL-6, IL-10, IL-17 and TNF-α were evaluated by ELISA in mice liver. Levels of pro-inflammatory cytokines, IL-1β, IL-6, IL-17 and TNF-α and also their respective mRNA increased in the liver from old mice. However, RSV decreased these levels in the case of IL-1β and TNF-α but only in old mice showing no effect on young and mature animals. This reduction was also found at the mRNA level. Levels of mRNA of the components of NALP-3 inflammasome, ASC, CASP-1, NALP-1 and NALP-3, also showed an age-dependent increase that was reversed by RSV. Furthermore, cyclooxygenase 2 levels, a marker of proinflammatory innate immune activity, were also upregulated in aged liver and reversed again by RSV. In conclusion, our study confirms that aging is accompanied by an increase in the proinflammatory pattern in the liver and that RSV reduces this pattern in old mice liver.
authors: Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide CSIC, CIBERER-Instituto de Salud San Carlos III, Carretera de Utrera Km 1, 41013 Sevilla, Spain; Departmento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: Bui Thanh
authors: BT
authors: Tung
authors: Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide CSIC, CIBERER-Instituto de Salud San Carlos III, Carretera de Utrera Km 1, 41013 Sevilla, Spain.
authors: Elisabeth
authors: E
authors: Rodríguez-Bies
authors: Departmento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: Elena
authors: E
authors: Talero
authors: Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide CSIC, CIBERER-Instituto de Salud San Carlos III, Carretera de Utrera Km 1, 41013 Sevilla, Spain.
authors: Enrique
authors: E
authors: Gamero-Estévez
authors: Departmento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: Virginia
authors: V
authors: Motilva
authors: Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide CSIC, CIBERER-Instituto de Salud San Carlos III, Carretera de Utrera Km 1, 41013 Sevilla, Spain.
authors: Plácido
authors: P
authors: Navas
authors: Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide CSIC, CIBERER-Instituto de Salud San Carlos III, Carretera de Utrera Km 1, 41013 Sevilla, Spain. Electronic address: glopllu@upo.es.
authors: Guillermo
authors: G
authors: López-Lluch
mesh_terms: D000375:Aging; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D016207:Cytokines; D012333:RNA, Messenger; D013267:Stilbenes; D000077185:Resveratrol
keywords: Aging; Cytokines; Inflammation; Liver; Resveratrol