title: Inhibition of autophagy suppresses sertraline-mediated primary ciliogenesis in retinal pigment epithelium cells.
abstract: Primary cilia are conserved cellular organelles that regulate diverse signaling pathways. Autophagy is a complex process of cellular degradation and recycling of cytoplasmic proteins and organelles, and plays an important role in cellular homeostasis. Despite its potential importance, the role of autophagy in ciliogenesis is largely unknown. In this study, we identified sertraline as a regulator of autophagy and ciliogenesis. Sertraline, a known antidepressant, induced the growth of cilia and blocked the disassembly of cilia in htRPE cells. Following treatment of sertraline, there was an increase in the number of cells with autophagic puncta and LC3 protein conversion. In addition, both a decrease of ATG5 expression and the treatment of an autophagy inhibitor resulted in the suppression of the sertraline-induced activation of autophagy in htRPE cells. Interestingly, we found that genetic and chemical inhibition of autophagy attenuated the growth of primary cilia in htRPE cells. Taken together, our results suggest that the inhibition of autophagy suppresses sertraline-induced ciliogenesis.
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Eun Sung
authors: ES
authors: Kim
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Ji Hyun
authors: JH
authors: Shin
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: So Jung
authors: SJ
authors: Park
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Yoon Kyung
authors: YK
authors: Jo
authors: Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea.
authors: Jae-Sung
authors: JS
authors: Kim
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Il-Hwan
authors: IH
authors: Kang
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Jung-Bum
authors: JB
authors: Nam
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Doo-Young
authors: DY
authors: Chung
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Yoonchul
authors: Y
authors: Cho
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: EunJoo H
authors: EH
authors: Lee
authors: Research Institute for Future Medicine Stem Cell & Regenerative Medicine Center, Samsung Medical Center, Seoul, Republic of Korea.
authors: Jong Wook
authors: JW
authors: Chang
authors: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Dong-Hyung
authors: DH
authors: Cho
mesh_terms: D001343:Autophagy / Q000187:drug effects*; D000071187:Autophagy-Related Protein 5; D002460:Cell Line; D002923:Cilia / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000187:drug effects*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*; D020280:Sertraline / Q000494:pharmacology*; D019098:Telomerase / Q000378:metabolism
chemical_list: D000071187:Autophagy-Related Protein 5; D008869:Microtubule-Associated Proteins; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline; D019098:Telomerase; C502778:ATG5 protein, human