title: Molecular cloning and sequence analysis of selenoprotein W gene and its mRNA expression patterns in response to metabolic status and cadmium exposure in goldfish, Carassius auratus.
abstract: Selenoprotein W (SelW) is a low molecular weight and selenocysteine containing protein with redox activity involved in the antioxidant response. In the present study, the full-length cDNA of goldfish (Carassius auratus) selenoprotein W (gfSelW) was successfully cloned from the liver tissue by rapid amplification of cDNA ends technique. The obtained gfSelW cDNA was 730 bp long with a 79 bp 5'-untranslated region (UTR), a 390 bp 3'-UTR containing the consensus polyadenylation signal AATAAA and a 261 bp open reading frame coding a protein of 86 amino acid residues. gfSelW mRNA was observed in all regions of brain and peripheral tissues by semi-quantitative RT-PCR, and the most abundant was detected in testis. After fasting for 1 week, gfSelW mRNA expression levels were significantly decreased compared to the fed group in hypothalamus and liver. After refeeding for 7 days, gfSelW mRNA expression levels were increased back. Furthermore, the mRNA expressions of gfSelW in hypothalamus and liver were varied in periprandial changes and significantly up-regulated after meal 2 h and 4 h, respectively. With cadmium exposure for 24 h, gfSelW mRNA expression levels in gill and leucocytes were significantly decreased at different cadmium concentrations changing from 0.5 ppm to 10 ppm. However, the gfSelW mRNA expression level was sharply increased in liver, relatively to the control about 4.98-fold at 0.5 ppm. The results in this study provide molecular characterization of SelW in goldfish and imply that SelW mRNA expression may be associated with metabolic status and oxidative stress and regulated by metabolic factors and cadmium in fish.
authors: Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, Henan 454000, China. Electronic address: chenwbfish@163.com.
authors: Wenbo
authors: W
authors: Chen
authors: Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, Henan 454000, China.
authors: Zhen
authors: Z
authors: Zhang
authors: Department of Medicine, Huzhou University, Huzhou, Zhejiang 313000, China.
authors: Haiyan
authors: H
authors: Dong
authors: Department of Biology, Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, Henan 454000, China.
authors: Xiaoxue
authors: X
authors: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D006054:Goldfish / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism*; D051151:Selenoprotein W / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D051151:Selenoprotein W; D002104:Cadmium
keywords: Cadmium; Carassius auratus; Fasting; Periprandial change; Selenoprotein W