title: The toxicity of NaF on BmN cells and a comparative proteomics approach to identify protein expression changes in cells under NaF-stress: impact of NaF on BmN cells.
abstract: Fluorides negatively affect the development of organisms and are a threat to human health and environmental safety. In this study, Bombyx mori N cell line (BmN) were used to explore effects of NaF on insect cells. We found that 8h (hrs) culture with high concentration of NaF (≥ 1 mM) induced significantly morphological changes. Dose-response curves of 72 h continuously cultured BmN treated with NaF showed that the half inhibitory concentration (IC50) value was 56.60 μM. Treatment of BmN with 100 and 300 μM of NaF induced apoptosis and necrosis. 2-D electrophoresis of whole cell extracted from BmN showed that treatment with 300 μM NaF up-regulated 32 proteins and down-regulated 11 proteins when compared with controls. We identified 5 different proteins by MALDI-TOF MS, and 4 of them were identified for the first time, including 2 up-regulated proteins (mitochondrial aldehyde dehydrogenase ALDH2 and prohibitin protein WPH) and 2 down-regulated proteins (calreticulin precursor CRT and DNA supercoiling factor SCF). These observations were further confirmed by fluorescence quantitative PCR. Together, our data suggest that these target proteins could be regarded as targets influenced by NaF and also provide clues for studies on the response metabolism pathway under NaF stress.
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Liang
authors: L
authors: Chen
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Huiqing
authors: H
authors: Chen
authors: Department of Stomatology, Zhenjiang First People's Hospital, Zhenjiang, Jiangsu 212013, PR China.
authors: Chun
authors: C
authors: Yao
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Cheng
authors: C
authors: Chang
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Hengchuan
authors: H
authors: Xia
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Chunxia
authors: C
authors: Zhang
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Yang
authors: Y
authors: Zhou
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
authors: Qin
authors: Q
authors: Yao
authors: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. Electronic address: kpchen@ujs.edu.cn.
authors: Keping
authors: K
authors: Chen
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D012831:Bombyx; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009336:Necrosis / Q000139:chemically induced; D009687:Nuclear Proteins / Q000378:metabolism; D000091402:Prohibitins; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D012969:Sodium Fluoride / Q000633:toxicity*
chemical_list: D002135:Calcium-Binding Proteins; D006360:Heat-Shock Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins; D012969:Sodium Fluoride; D000444:Aldehyde Dehydrogenase
keywords: Apoptosis; Fluoride; Necrosis; Silkworm; Two-dimensional electrophoresis