title: Dentin and pulp sense cold stimulus.
abstract: Dentin hypersensitivity is a common symptom, and recent convergent evidences have reported transient receptor potential (TRP) channels in odontoblasts act as mechanical and thermal molecular sensor, which detect stimulation applied on the exposed dentin surface, to drive multiple odontoblastic cellular functions, such as sensory transduction and/or dentin formation. In the present study, we confirmed expression of TRP melastatin subfamily member-8 (TRPM8) channels in primary cultured cells derived from human dental pulp cells (HPCs) and mouse odontoblast-lineage cells (OLCs) as well as in dentin matrix protein-1 (DMP-1) and dentin sialoprotein (DSP) positive acutely isolated rat odontoblasts from dental pulp tissue slice culture by immunohistochemical analyses. In addition, we detected TRPM8 channel expression on HPCs and OLCs by RT-PCR and Western blotting analyses. These results indicated that both odontoblasts and dental pulp cells express TRPM8 channels in rat, mouse and human, and therefore we hypothesize they may contribute as cold sensor in tooth.
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan. Electronic address: mas-tok@dent.kagoshima-u.ac.jp.
authors: Masayuki
authors: M
authors: Tokuda
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors: Shoko
authors: S
authors: Tatsuyama
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors: Mari
authors: M
authors: Fujisawa
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors: Yoko
authors: Y
authors: Morimoto-Yamashita
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors: Yoshiko
authors: Y
authors: Kawakami
authors: Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan.
authors: Yoshiyuki
authors: Y
authors: Shibukawa
authors: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors: Mistuso
authors: M
authors: Torii
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003080:Cold Temperature / Q000009:adverse effects*; D003782:Dental Pulp / Q000502:physiology*; D003807:Dentin Sensitivity / Q000503:physiopathology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D009804:Odontoblasts / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050053:TRPM Cation Channels / Q000378:metabolism*
chemical_list: D050053:TRPM Cation Channels; C425966:TRPM8 protein, human