title: Involvement of major facilitator superfamily proteins SfaA and SbnD in staphyloferrin secretion in Staphylococcus aureus.
abstract: A paucity of information exists concerning the mechanism(s) by which bacteria secrete siderophores into the extracellular compartment. We investigated the role of SfaA and SbnD, two major facilitator superfamily (MFS)-type efflux proteins, in the secretion of the Staphylococcus aureus siderophores staphyloferrin A (SA) and staphyloferrin B (SB), respectively. Deletion of sfaA resulted in a drastic reduction of SA secreted into the supernatant with a corresponding accumulation of SA in the cytoplasm and a significant growth defect in cells devoid of SB synthesis. In contrast, sbnD mutants showed transiently lowered levels of secreted SB, suggesting the involvement of additional efflux mechanisms.
authors: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
authors: Mélissa
authors: M
authors: Hannauer
authors: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
authors: Jessica R
authors: JR
authors: Sheldon
authors: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada; Centre for Human Immunology, University of Western Ontario, London, Ontario N6A 5C1 Canada. Electronic address: deh@uwo.ca.
authors: David E
authors: DE
authors: Heinrichs
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002951:Citrates / Q000378:metabolism*; D055786:Gene Knockout Techniques; D026901:Membrane Transport Proteins / Q000502:physiology*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D009154:Mutation; D009952:Ornithine / Q000031:analogs & derivatives* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002951:Citrates; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009952:Ornithine; C083439:staphyloferrin B; C063851:staphyloferrin A; C075040:NorA protein, Staphylococcus
keywords: Efflux; Iron acquisition; Siderophores