title: Effect on β-galactosidase synthesis and burden on growth of osmotic stress in Escherichia coli.
abstract: Osmotic Shock is known to negatively affect growth rate along with an extended lag phase. The reduction in growth rate can be characterized as burden due to the osmotic stress. Studies have shown that production of unnecessary protein also burdens cellular growth. This has been demonstrated by growing Escherichia coli on glycerol in the presence of Isopropyl-β-D-1-thiogalactopyranoside (IPTG) to induce β-galactosidase synthesis which does not offer any benefit towards growth. The trade off between osmotic stress and burden on growth due to unnecessary gene expression has not been enumerated. The influence of osmotic stress on β-galactosidase synthesis and activity is not clearly understood. Here, we study the effect of salt concentration on β-galactosidase activity and burden on growth due to unnecessary gene expression in E.coli. We characterize the burden on growth in presence of varying concentrations of salt in the presence of IPTG using three strains, namely wild type, ∆lacI and ∆lacIlacZ mutant strains. We demonstrate that the salt concentrations, sensitively inhibits enzyme synthesis thereby influencing the burden on growth. In a wild type strain, addition of lactose into the medium demonstrated growth benefit at low salt concentration but not at higher concentrations. The extent of burden due to osmotic shock was higher in a lactose M9 medium than in a glycerol M9 medium. A linear relationship was observed between enzyme activity and burden on growth in various media types studied.
authors: Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors: Pushkar
authors: P
authors: Malakar
authors: Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India.
authors: Vivek K
authors: VK
authors: Singh
authors: Department of Chemical Engineering, IIT Bombay, Mumbai, 400076 India.
authors: Richa
authors: R
authors: Karmakar
authors: Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 Maharashtra India ; Department of Chemical Engineering, IIT Bombay, Mumbai, 400076 India.
authors: Kareenhalli V
authors: KV
authors: Venkatesh
keywords: Escherichia coli; Growth rate; IPTG; Osmotic stress; Unnecessary Gene Expression