title: Intermittent Fluorescence Oscillations in Lipid Droplets in a Live Normal and Lung Cancer Cell: Time-Resolved Confocal Microscopy.
abstract: Intermittent structural oscillation in the lipid droplets of live lung cells is monitored using time-resolved confocal microscopy. Significant differences are observed between the lung cancer cell (A549) and normal (nonmalignant) lung cell (WI38). For this study, the lipid droplets are covalently labeled with a fluorescent dye, coumarin maleimide (7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin, CPM). The number of lipid droplets in the cancer cell is found to be ∼20-fold higher than that in the normal (nonmalignant) cell. The fluctuation in the fluorescence intensity of the dye (CPM) is attributed to the red-ox processes and periodic formation/rupture of the S-CPM bond. The amount of reactive oxygen species (ROS) is much higher in a cancer cell. This is manifested in faster oscillations (0.9 ± 0.3 s) in cancer cells compared to that in the normal cells (2.8 ± 0.7 s). Solvation dynamics in the lipid droplets of cancer cells is slower compared to that in the normal cell.
authors: Department of Physical Chemistry, Indian Association for the Cultivation of Science , Jadavpur, Kolkata 700032, India.
authors: Rajdeep
authors: R
authors: Chowdhury
authors: Department of Physical Chemistry, Indian Association for the Cultivation of Science , Jadavpur, Kolkata 700032, India.
authors: Md Asif
authors: MA
authors: Amin
authors: Department of Physical Chemistry, Indian Association for the Cultivation of Science , Jadavpur, Kolkata 700032, India.
authors: Kankan
authors: K
authors: Bhattacharyya
mesh_terms: D003374:Coumarins / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D066292:Lipid Droplets / Q000648:ultrastructure*; D008168:Lung / Q000648:ultrastructure*; D008175:Lung Neoplasms / Q000473:pathology*; D018613:Microscopy, Confocal; D015394:Molecular Structure; D017382:Reactive Oxygen Species / Q000737:chemistry; D012015:Reference Standards
chemical_list: D003374:Coumarins; D017382:Reactive Oxygen Species; C030860:N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide