title: MicroRNA-584 functions as a tumor suppressor and targets PTTG1IP in glioma.
abstract: MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression at the post transcriptional level. Compelling evidence shows that there are causative links between miRNAs deregulation and cancer development and progression. In this study, we demonstrated that miR-584 was downregulated in human glioma and could suppress growth of the human glioma cell line U87-MG and U251-MG. Bioinformatics analysis indicated that PTTG1IP was a putative target of miR-584. In a Luciferase reporter system, we confirmed that PTTG1IP was a direct target gene of miR-584. These findings indicate that miR-584 suppresses glioma cell growth by negatively regulating the expression of PTTG1IP, suggesting that miR-584 has a tumor suppressive role in human glioma pathogenesis.
authors: Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University Yunnan 650032, China.
authors: Xiang-Peng
authors: XP
authors: Wang
authors: Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University Yunnan 650032, China.
authors: Xing-Li
authors: XL
authors: Deng
authors: Institute of Neuroscience, Kunming Medical University Yunnan 650050, China.
authors: Li-Yan
authors: LY
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C552781:MIRN584 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C112633:PTTG1IP protein, human
keywords: Glioma; PTTG1IP; miR-584; microRNA