title: Exposure to acids changes the proteomic of acquired dentine pellicle.
abstract: OBJECTIVE
  For the first time, this study characterized the proteome of the acquired pellicle formed on human dentine. The changes in this proteome after exposure to lactic or citric acid were also evaluated.
  
  
  METHODS
  Volunteers (n=9) wore a mandibular device containing 6 specimens of human root dentine. After the device remained in the volunteers' oral cavities for 10min or 2h to allow the formation of the acquired pellicle in situ, the specimens were immersed in citric acid (1%, pH 2.5) or lactic acid (0.1M, pH 4.8) or deionized water for 20s. In sequence, the pellicle was collected with an electrode filter paper soaked in 3% citric acid. This procedure was repeated for two additional days following a crossover protocol. After harvest, proteins were subjected to reverse phase liquid chromatography coupled to mass spectrometry (nLC-ESI-MS/MS). MS/MS data were processed and submitted to Proteome Discoverer software. Searches were done using SWISS-PROT and TrEMBL databases for human proteins.
  
  
  RESULTS
  In total, 223 distinct proteins were identified in the dentine acquired pellicle in each of the different conditions. Exposure to citric acid dramatically reduced the number of identified proteins. This did not occur for lactic acid. Acid-resistant proteins, such as mucins, were identified after pellicle was exposed to lactic or citric acid.
  
  
  CONCLUSIONS
  These proteins could be related to protective effect of tooth homeostasis. Moreover, in the future, they could be candidates to the development of a supplemental therapy for the prevention and treatment of dental caries and dental erosion.
  
  
  CONCLUSIONS
  This study indicates some acid-resistant proteins that could be used in dental products to prevent dental caries and erosion.
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Taísa Ribas
authors: TR
authors: Delecrode
authors: Schulich School of Medicine & Dentistry, Western University, Dental Sciences Building - DSB0071, N6A 5C1 London, ON, Canada. Electronic address: mbuzalaf@fob.usp.br.
authors: Walter Luiz
authors: WL
authors: Siqueira
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Flávia Cardoso
authors: FC
authors: Zaidan
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Melina Rodrigues
authors: MR
authors: Bellini
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Aline Lima
authors: AL
authors: Leite
authors: Schulich School of Medicine & Dentistry, Western University, Dental Sciences Building - DSB0071, N6A 5C1 London, ON, Canada.
authors: Yishi
authors: Y
authors: Xiao
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Daniela
authors: D
authors: Rios
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
authors: Ana Carolina
authors: AC
authors: Magalhães
authors: Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro, Brisolla, 9-75, 17012-901 Bauru, SP, Brazil. Electronic address: walter.siqueira@uwo.ca.
authors: Marília Afonso Rabelo
authors: MA
authors: Buzalaf
mesh_terms: D000293:Adolescent; D000328:Adult; D019343:Citric Acid / Q000494:pharmacology*; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism; D044622:Dental Pellicle / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000494:pharmacology*; D008334:Mandible / Q000033:anatomy & histology; D008964:Molar, Third; D009077:Mucins / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D055815:Young Adult
chemical_list: D009077:Mucins; D020543:Proteome; D019343:Citric Acid; D019344:Lactic Acid
keywords: Acidic exposure; Acquired pellicle; Dentine; Proteomics