title: Tumor progression is associated with increasing CD11b+ cells and CCL2 in Lewis rat sarcoma.
abstract: Tumor models are essential for basic anticancer research and development of novel therapies. In this study, we used a rat sarcoma model in which subcutaneous tumor develops after D6 cell inoculation. The aim of the current study was to analyze changes in haematological parameters, immune cell sub-populations and cytokine profiling during tumor growth, after tumor excision and after second inoculation of D6 cells. Tumor progression was found to be associated with an increased number of leukocytes and increased proportion of CD11b+ cells in peripheral blood. Serum concentration of chemokine (c-c motif) ligand 2, L-selectin and intra cellular adhesion molecule-1 also increased with growing tumor. However, the proportion of CD4+, CD8+ and MHC II+ cells decreased with growth of tumors. After tumor excision, all these parameters returned to pre-inoculation levels and did not change even after a second inoculation of D6 cells. Moreover, absence of secondary tumors after second inoculation of D6 cells gives an insight into development of antitumor immunity stimulated by primary tumor.
authors: Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic mishra@iapg.cas.cz.
authors: Rajbardhan
authors: R
authors: Mishra
authors: Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic.
authors: Jana
authors: J
authors: Kovalska
authors: Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic.
authors: Jozef
authors: J
authors: Janda
authors: Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic Institute of Microbiology AS CR, v.v.i., Prague, Czech Republic.
authors: Luca
authors: L
authors: Vannucci
authors: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic.
authors: Radko
authors: R
authors: Rajmon
authors: Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic.
authors: Vratislav
authors: V
authors: Horak
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000097:blood*; D018932:Chemokine CCL2 / Q000097:blood*; D018450:Disease Progression; D005434:Flow Cytometry; D007964:Leukocytosis; D051381:Rats; D011917:Rats, Inbred Lew; D012513:Sarcoma, Experimental / Q000097:blood / Q000473:pathology*
chemical_list: D039481:CD11b Antigen; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2
keywords: CCL2; CD11b+ cells; Lewis rat sarcoma; neutrophils; tumor immunology