title: Fine mapping of a major quantitative trait locus, qLG-9, that controls seed longevity in rice (Oryza sativa L.).
abstract: We fine-mapped a quantitative trait locus, qLG - 9, for seed longevity detected between Japonica-type and Indica-type cultivars. qLG - 9 was mapped in a 30-kb interval of the Nipponbare genome sequence. A quantitative trait locus, qLG-9, for seed longevity in rice has previously been detected on chromosome 9 by using backcross inbred lines derived from a cross between Japonica-type (Nipponbare) and Indica-type (Kasalath) cultivars. In the present study, the chromosomal location of qLG-9 was precisely determined by fine-scale mapping. Firstly, allelic difference in qLG-9 was verified by QTL analysis of an F2 population derived from a cross between Nipponbare and NKSL-1, in which a segment of Kasalath chromosome 9 was substituted in Nipponbare genetic background. Then, we selected F2 plants in which recombination had occurred near qLG-9 and performed F3 progeny testing on these plants to determine the genotype classes of qLG-9. Eventually, qLG-9 was mapped in a 30-kb interval (defined by two markers, CAPSb and CHPa12) of the Nipponbare genome sequence. This allowed us to nominate positional candidate genes of qLG-9. Additionally, we developed near-isogenic lines (NIL) for qLG-9 by marker-assisted selection. qLG-9 NIL showed significantly higher seed longevity than isogenic control of Nipponbare. These results will facilitate cloning of the gene(s) underlying qLG-9 as well as marker-assisted transfer of desirable genes for seed longevity improvement in rice.
authors: Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan, sasaki@isas.a.u-tokyo.ac.jp.
authors: K
authors: K
authors: Sasaki
authors: Y
authors: Y
authors: Takeuchi
authors: K
authors: K
authors: Miura
authors: T
authors: T
authors: Yamaguchi
authors: T
authors: T
authors: Ando
authors: T
authors: T
authors: Ebitani
authors: A
authors: A
authors: Higashitani
authors: T
authors: T
authors: Yamaya
authors: M
authors: M
authors: Yano
authors: T
authors: T
authors: Sato
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D040641:Quantitative Trait Loci; D012639:Seeds / Q000254:growth & development*; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers