title: Challenge with lipopolysaccharides or Freund's adjuvant? What is the best option to trigger acute phase protein production in broilers?
abstract: Broilers were injected at 10 days of age with either Escherichia coli lipopolysaccharides (LPS) or with Freund's adjuvants (FA) to investigate its triggering effect on the acute phase reaction (APR). First the kinetics of certain APP was studied by sampling blood 4 h, 8 h, 12 h and 24 h post injection with LPS. Ovotransferrin (OVT) and α-1 acid glycoprotein (AGP) concentration increased with time post injection (PI) with LPS to reach a plateau at 12 and 24 h PI. Caeruloplasmin (CP) did not increase with time PI. Compared to injection with phosphate buffered saline, OVT concentrations were higher when injecting chicks with LPS at all time points PI. At 24 h PI, LPS injection resulted in higher OVT and AGP concentration compared to injection with FA. It is recommended to use LPS instead of FA to trigger the APR. The best time point to sample blood for APP determination is 24 h PI.
authors: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenber 30 Box 2456, Leuven 3001, Belgium; Animal Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Scheldeweg 68, Melle 9090, Belgium. Electronic address: astrid_koppenol@hotmail.com.
authors: A
authors: A
authors: Koppenol
authors: Animal Science Unit, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, Gembloux 5030, Belgium.
authors: N
authors: N
authors: Everaert
authors: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenber 30 Box 2456, Leuven 3001, Belgium.
authors: J
authors: J
authors: Buyse
authors: Animal Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Scheldeweg 68, Melle 9090, Belgium.
authors: E
authors: E
authors: Delezie
mesh_terms: D000209:Acute-Phase Proteins / Q000276:immunology* / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000276:immunology / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D002645:Chickens / Q000276:immunology* / Q000378:metabolism*; D003207:Conalbumin / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000276:immunology*; D007279:Injections, Subcutaneous; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D009961:Orosomucoid / Q000378:metabolism; D013997:Time Factors
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; D008070:Lipopolysaccharides; D009961:Orosomucoid; D003207:Conalbumin; D005620:Freund's Adjuvant; D002570:Ceruloplasmin
keywords: Acute phase protein kinetics; Acute phase reaction; Caeruloplasmin; Ovotransferrin; α-1 Acid glycoprotein