title: Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus.
abstract: This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.
authors: Molecular Breeding and Biodiversity Research Group, Department of Genetics, Stellenbosch University, JC Smuts Building, van der Bijl Street, Stellenbosch 7600, South Africa; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Dublin Road, Galway, Ireland.
authors: L
authors: L
authors: Mirimin
authors: R
authors: R
authors: Roodt-Wilding
mesh_terms: D000818:Animals; D017756:Aquaculture; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D007814:Larva / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats; D010473:Perciformes / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015053:Zygote
chemical_list: D004247:DNA
keywords: F1; Kabeljou; first generation; genomic DNA isolation; microsatellite loci; pedigree analyses