title: Increased endogenous DNA oxidation correlates to increased iron levels in melanocytes relative to keratinocytes.
abstract: The endogenous oxidative state of normal human epidermal melanocytes was investigated and compared to normal human epidermal keratinocytes (NHEKs) in order to gain new insight into melanocyte biology. Previously, we showed that NHEKs contain higher levels of hydrogen peroxide (H2O2) than melanocytes and that it can migrate from NHEKs to melanocytes by passive permeation. Nevertheless, despite lower concentrations of H2O2, we now report higher levels of oxidative DNA in melanocytes as indicated by increased levels of 8-oxo-2'-deoxyguanosine (8-oxo-dG): 4.49 (±0.55 SEM) 8-oxo-dG/10(6) dG compared to 1.49 (±0.11 SEM) 8-oxo-dG/10(6) dG for NHEKs. An antioxidant biomarker, glutathione (GSH), was also lower in melanocytes (3.14 nmoles (±0.15 SEM)/cell) in comparison to NHEKs (5.98 nmoles (±0.33 SEM)/cell). Intriguingly, cellular bioavailable iron as measured in ferritin was found to be nearly fourfold higher in melanocytes than in NHEKs. Further, ferritin levels in melanocytes were also higher than in hepatocarcinoma cells, an iron-rich cell, and it indicates that higher relative iron levels may be characteristic of melanocytes. To account for the increased oxidative DNA and lower GSH and H2O2 levels that we observe, we propose that iron may contribute to higher levels of oxidation by reacting with H2O2 through a Fenton reaction leading to the generation of DNA-reactive hydroxyl radicals. In conclusion, our data support the concept of elevated oxidation and high iron levels as normal parameters of melanocytic activity. We present new evidence that may contribute to our understanding of the melanogenic process and lead to the development of new skin care products.
authors: Edward
authors: E
authors: Pelle
authors: Xi
authors: X
authors: Huang
authors: Qi
authors: Q
authors: Zhang
authors: Nadine
authors: N
authors: Pernodet
authors: Daniel B
authors: DB
authors: Yarosh
authors: Krystyna
authors: K
authors: Frenkel
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008544:Melanocytes / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D004247:DNA; D007501:Iron; D005978:Glutathione