title: Plasma thiols levels in Alzheimer's disease mice under diet-induced hyperhomocysteinemia: effect of S-adenosylmethionine and superoxide-dismutase supplementation.
abstract: Widely confirmed reports were published on association between hyperhomocysteinemia, B vitamin deficiency, oxidative stress, and amyloid-β in Alzheimer's disease (AD). Homocysteine, cysteine, cysteinylglycine and glutathione are metabolically interrelated thiols that may be potential indicators of health status and disease risk; they all participate in the metabolic pathway of homocysteine. Previous data obtained in one of our laboratories showed that B vitamin deficiency induced exacerbation of AD-like features in TgCRND8 AD mice; these effects were counteracted by S-adenosylmethionine (SAM) supplementation, through the modulation of DNA methylation and antioxidant pathways. Since the cellular response to oxidative stress typically involves alteration in thiols content, a rapid and sensitive HPLC method with fluorescence detection was here used to evaluate the effect of SAM and superoxide-dismutase (SOD) supplementation on thiols level in plasma, in TgCRND8 mice. The quantitative data obtained from HPLC analysis of mice plasma samples showed significant decrease of thiols level when the B vitamin deficient diet was supplemented with SAM + SOD and SOD alone, the latter showing the greatest effect. All these considerations point out the measurement of plasma thiols concentration as a powerful tool of relevance for all clinical purposes involving the evaluation of oxidative stress. The coupling of HPLC with fluorimetric detection, here used, provided a strong method sensitivity allowing thiols determination at very low levels.
authors: Istituto di Biochimica e Biochimica Clinica, Universitá Cattolica del Sacro Cuore, Rome, Italy.
authors: Silvia
authors: S
authors: Persichilli
authors: Istituto di Biochimica e Biochimica Clinica, Universitá Cattolica del Sacro Cuore, Rome, Italy.
authors: Jacopo
authors: J
authors: Gervasoni
authors: Department of Surgery "P. Valdoni", Sapienza University of Rome, Rome, Italy.
authors: Alessandra
authors: A
authors: Di Napoli
authors: Department of Psychology, Section of Neuroscience, Sapienza University of Rome, Rome, Italy European Center for Brain Research (CERC)/IRCCS Santa Lucia Foundation, Rome, Italy.
authors: Andrea
authors: A
authors: Fuso
authors: Department of Surgery "P. Valdoni", Sapienza University of Rome, Rome, Italy.
authors: Vincenzina
authors: V
authors: Nicolia
authors: Istituto di Biochimica e Biochimica Clinica, Universitá Cattolica del Sacro Cuore, Rome, Italy Istituto di Chimica del Riconoscimento Molecolare - UOS Roma, Consiglio Nazionale delle Ricerche c/o Istituto di Biochimica e Biochimica Clinica, Universitá Cattolica del Sacro Cuore, Rome, Italy Centro di Ricerca in Biochimica e Nutrizione dello Sport (CriBeNS), Universitá Cattolica del Sacro Cuore, Milano, Italy.
authors: Bruno
authors: B
authors: Giardina
authors: Department of Surgery "P. Valdoni", Sapienza University of Rome, Rome, Italy.
authors: Sigfrido
authors: S
authors: Scarpa
authors: Istituto di Chimica del Riconoscimento Molecolare - UOS Roma, Consiglio Nazionale delle Ricerche c/o Istituto di Biochimica e Biochimica Clinica, Universitá Cattolica del Sacro Cuore, Rome, Italy.
authors: Claudia
authors: C
authors: Desiderio
authors: Department of Surgery "P. Valdoni", Sapienza University of Rome, Rome, Italy.
authors: Rosaria A
authors: RA
authors: Cavallaro
mesh_terms: D000544:Alzheimer Disease / Q000097:blood* / Q000178:diet therapy* / Q000235:genetics; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005978:Glutathione / Q000097:blood; D006710:Homocysteine / Q000097:blood; D006801:Humans; D020138:Hyperhomocysteinemia / Q000097:blood / Q000139:chemically induced*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D012436:S-Adenosylmethionine / Q000627:therapeutic use*; D013438:Sulfhydryl Compounds / Q000097:blood*; D013482:Superoxide Dismutase / Q000627:therapeutic use*
chemical_list: D016564:Amyloid beta-Protein Precursor; D013438:Sulfhydryl Compounds; D006710:Homocysteine; D012436:S-Adenosylmethionine; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Alzheimer's disease; HPLC; S-adenosylmethionine; homocysteine; superoxide-dismutase; thiols