title: Magnetosomes, biogenic magnetic nanomaterials for brain molecular imaging with 17.2 T MRI scanner.
abstract: The fast development of sensitive molecular diagnostic tools is currently paving the way for a personalized medicine. A new class of ultrasensitive magnetic resonance imaging (MRI) T₂-contrast agents based on magnetosomes, magnetite nanocrystals biomineralized by magnetotactic bacteria, is proposed here. The contrast agents can be injected into the blood circulation and detected in the picomolar range. Purified magnetosomes are water-dispersible and stable within physiological conditions and exhibit at 17.2 T a transverse relaxivity r₂ four times higher than commercial ferumoxide. The subsequent gain in sensitivity by T₂(*) -weighted imaging at 17.2 T of the mouse brain vasculature is evidenced in vivo after tail vein injection of magnetosomes representing a low dose of iron (20 μmoliron kg(-1)), whereas no such phenomenon with the same dose of ferumoxide is observed. Preclinical studies of human pathologies in animal models will benefit from the combination of high magnetic field MRI with sensitive, low dose, easy-to-produce biocompatible contrast agents derived from bacterial magnetosomes.
authors: CEA/DSV/I2BM/NeuroSpin/UNIRS, CEA Saclay, 91191, Gif-sur-Yvette Cedex, France.
authors: Sébastien
authors: S
authors: Mériaux
authors: CEA/DSV/I2BM/NeuroSpin/UNIRS, CEA Saclay, 91191, Gif-sur-Yvette Cedex, France.
authors: Marianne
authors: M
authors: Boucher
authors: Institute of Myology (NMR Laboratory), CEA/DSV/I2BM/MIRCen (IdM NMR Laboratory), UPMC Paris 6 University, 75013, Paris, France.
authors: Benjamin
authors: B
authors: Marty
authors: Paris 13 University/Sorbonne Paris Cité/CSPBAT/CNRS (UMR7244), 93017, Bobigny Cedex, France.
authors: Yoann
authors: Y
authors: Lalatonne
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Sandra
authors: S
authors: Prévéral
authors: Paris 13 University/Sorbonne Paris Cité/CSPBAT/CNRS (UMR7244), 93017, Bobigny Cedex, France.
authors: Laurence
authors: L
authors: Motte
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Christopher T
authors: CT
authors: Lefèvre
authors: CEA/DSV/I2BM/NeuroSpin/UNIRS, CEA Saclay, 91191, Gif-sur-Yvette Cedex, France.
authors: Françoise
authors: F
authors: Geffroy
authors: CEA/DSV/I2BM/NeuroSpin/UNIRS, CEA Saclay, 91191, Gif-sur-Yvette Cedex, France.
authors: Franck
authors: F
authors: Lethimonnier
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Michel
authors: M
authors: Péan
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Daniel
authors: D
authors: Garcia
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Géraldine
authors: G
authors: Adryanczyk-Perrier
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: David
authors: D
authors: Pignol
authors: CEA/Aix-Marseille University/CNRS (UMR7265), DSV/IBEB/SBVME/LBC, CEA Cadarache, 13108, Saint-Paul-lez-Durance Cedex, France.
authors: Nicolas
authors: N
authors: Ginet
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008280:Magnetics / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry; D055944:Magnetosomes / Q000737:chemistry* / Q000378:metabolism; D042201:Magnetospirillum / Q000378:metabolism; D051379:Mice; D057054:Molecular Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides; D052203:Ferrosoferric Oxide
keywords: brain molecular imaging; contrast agents; magnetosome