title: Anti-apoptosis effects on hearts of SHSST cyclodextrin complex in a carbon tetrachloride-induced cirrhotic cardiomyopathy rat model.
abstract: Cirrhotic cardiomyopathy (CCM) is a common cardiac dysfunction in patients waiting for orthotopic liver transplantation (OLT). Carbon tetrachloride (CCl₄) intraperitoneal (IP) injection has been reported as successful in a cirrhosis-induced CCM model. In this work, we used the same assay for CCM induction using CCl₄ (0.2 mg/kg) IP injection twice per day for 14 days during the cardiac protection drugs treatment process. The cardiac protection drugs were silymarin (100 mg/kg/day), baicalein (30 mg/kg/day), San Huang Shel Shin Tang (SHSST, 30 mg/kg/day) and β-cyclodextrin modified SHSST (SHSSTc, 30 mg/kg/day and 300 mg/kg/day). After 4 weeks of treatment, the SHSSTc cardiac protection effects were determined through activation of the IGF1R cell survival pathway and inhibition of Fas-FADD death domain induced-apoptosis. SHSSTc cardiac protection was enhanced through β-cyclodextrin modification, which increased bio-availability, and displayed stronger protective effects than silymarin and baicalein, both of which are well-known liver protection drugs. Thus, SHSSTc might provide the best therapeutic benefit in CCM treatment.
authors: Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Cheng-Hsun
authors: CH
authors: Yang
authors: Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Wei-Jen
authors: WJ
authors: Ting
authors: Division of Cardiology, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.
authors: Pei-Ying
authors: PY
authors: Pai
authors: Department of Laboratory, Tsaotun Psychiatric Center, Ministry of Health and Welfare, Nantou 54249, Taiwan, Republic of China.
authors: Sheng-Huang
authors: SH
authors: Chang
authors: Chinese Medicine Department, China Medical University Beigang Hospital, Yunlin 63244, Taiwan, Republic of China.
authors: Tsung-Jung
authors: TJ
authors: Ho
authors: Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan, Republic of China.
authors: Jing-Ying
authors: JY
authors: Lin
authors: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Fuu-Jen
authors: FJ
authors: Tsai
authors: Department of Biotechnology, Bharathiar University , Coimbatore 641046, India.
authors: Viswanadha Vijaya
authors: VV
authors: Padama
authors: Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Yuhsin
authors: Y
authors: Tsai
authors: Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan, Republic of China.
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002251:Carbon Tetrachloride; D009202:Cardiomyopathies / Q000188:drug therapy* / Q000473:pathology; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D008106:Liver Cirrhosis, Experimental / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012838:Silymarin / Q000494:pharmacology
chemical_list: D002316:Cardiotonic Agents; D003505:Cyclodextrins; D044950:Flavanones; D012838:Silymarin; C006680:baicalein; D002251:Carbon Tetrachloride