title: Differential gene expression analysis of benzo(a)pyrene toxicity in the clam, Ruditapes philippinarum.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are known for their carcinogenic, teratogenic and mutagenic properties. Benzo(a)pyrene (BaP) possesses the greatest carcinogenic potential among the various PAHs. In this study, digital gene expression (DGE) was performed to investigate the digestive gland transcriptome profile of the clam Ruditapes philippinarum exposed to BaP. A total of 10,508,312 and 11,414,297 clean reads were generated respectively, from control and BaP exposure DGE libraries. One hundred and forty-five differentially expressed genes were detected after comparing two libraries with 58 up-regulated and 87 down-regulated genes. GO annotation and KEGG pathway analyses were performed on all genes to understand their biological functions and processes. The results showed that numerous enriched differentially expressed genes are related to growth and development, antioxidant metabolism, apoptosis and detoxification metabolism. Quantitative real-time PCR was performed to verify the expressed genes of DGE. Our results provide evidences that RNA-seq is a powerful tool for toxicology and capable of generating novel and valuable information at the transcriptome level for characterizing deleterious effects caused by BaP.
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors: Tong
authors: T
authors: Liu
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China. Electronic address: panlq@ouc.edu.cn.
authors: Luqing
authors: L
authors: Pan
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors: Qian
authors: Q
authors: Jin
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors: Yuefeng
authors: Y
authors: Cai
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D049872:Bivalvia / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity*; D020869:Gene Expression Profiling; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene
keywords: Benzo(a)pyrene; Differentially expressed genes; Digital gene expression sequencing; Quantitative real-time PCR; Ruditapes philippinarum