title: Viral-inducible Argonaute18 confers broad-spectrum virus resistance in rice by sequestering a host microRNA.
abstract: Viral pathogens are a major threat to rice production worldwide. Although RNA interference (RNAi) is known to mediate antiviral immunity in plant and animal models, the mechanism of antiviral RNAi in rice and other economically important crops is poorly understood. Here, we report that rice resistance to evolutionarily diverse viruses requires Argonaute18 (AGO18). Genetic studies reveal that the antiviral function of AGO18 depends on its activity to sequester microRNA168 (miR168) to alleviate repression of rice AGO1 essential for antiviral RNAi. Expression of miR168-resistant AGO1a in ago18 background rescues or increases rice antiviral activity. Notably, stable transgenic expression of AGO18 confers broad-spectrum virus resistance in rice. Our findings uncover a novel cooperative antiviral activity of two distinct AGO proteins and suggest a new strategy for the control of viral diseases in rice.
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Jianguo
authors: J
authors: Wu
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Zhirui
authors: Z
authors: Yang
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Yu
authors: Y
authors: Wang
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Lijia
authors: L
authors: Zheng
authors: Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, College of Life Sciences, Tsinghua University, Beijing, China.
authors: Ruiqiang
authors: R
authors: Ye
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors: Yinghua
authors: Y
authors: Ji
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Shanshan
authors: S
authors: Zhao
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Shaoyi
authors: S
authors: Ji
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Ruofei
authors: R
authors: Liu
authors: Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, College of Life Sciences, Tsinghua University, Beijing, China.
authors: Le
authors: L
authors: Xu
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Hong
authors: H
authors: Zheng
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors: Yijun
authors: Y
authors: Zhou
authors: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Xin
authors: X
authors: Zhang
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Beijing, China.
authors: Xiaofeng
authors: X
authors: Cao
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
authors: Lianhui
authors: L
authors: Xie
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
authors: Zujian
authors: Z
authors: Wu
authors: Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, College of Life Sciences, Tsinghua University, Beijing, China.
authors: Yijun
authors: Y
authors: Qi
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
authors: Yi
authors: Y
authors: Li
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012275:Oryza / Q000235:genetics / Q000821:virology*; D010942:Plant Viruses / Q000472:pathogenicity
chemical_list: D060565:Argonaute Proteins
keywords: RNAi; infectious disease; microbiology; plant biology; rice; viral disease; viruses