title: Evaluation of nanoformulated therapeutics in an ex-vivo bovine corneal irritation model.
abstract: OBJECTIVE
  To determine the internalization and protective effects of potential ophthalmic formulations and nanoformulated natural proteins in ex-vivo bovine corneal alkali burn model.
  
  
  METHODS
  The bovine cornea obtained were subjected to the 0.5N NaOH insult that induced alkali burn and inflammation as observed in the in vivo situation. The toxic effects of the nanoformulation were evaluated in the normal and insult induced cornea using histological analysis. Internalization studies were carried out using in vivo imaging and analysis (IVIS, PerkinElmer, USA).
  
  
  RESULTS
  The nanoformulations employed in this study showed no obvious changes in the integrity of the cornea. Further, improvements in the light transmittance and reduced inflammation were observed. The IVIS showed a dose dependant increase in the uptake of the nanoformulations with time.
  
  
  CONCLUSIONS
  The nanoformulated bovine lactoferrin and SurR9-C84A (SR9) proteins evaluated in the ex vivo bovine corneal irritation model is the first of its kind, and we report here the non-toxic and therapeutic potential of these formulations for topical applications.
authors: Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), Molecular and Medical Research (MMR) Strategic Research Centre, School of Medicine (SoM), Faculty of Health, Deakin University, Waurn Ponds, Victoria 3217, Australia.
authors: Sriramoju
authors: S
authors: Bhasker
authors: Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), Molecular and Medical Research (MMR) Strategic Research Centre, School of Medicine (SoM), Faculty of Health, Deakin University, Waurn Ponds, Victoria 3217, Australia.
authors: Roy
authors: R
authors: Kislay
authors: Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), Molecular and Medical Research (MMR) Strategic Research Centre, School of Medicine (SoM), Faculty of Health, Deakin University, Waurn Ponds, Victoria 3217, Australia.
authors: Kanwar K
authors: KK
authors: Rupinder
authors: Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), Molecular and Medical Research (MMR) Strategic Research Centre, School of Medicine (SoM), Faculty of Health, Deakin University, Waurn Ponds, Victoria 3217, Australia. Electronic address: jagat.kanwar@deakin.edu.au.
authors: Kanwar R
authors: KR
authors: Jagat
mesh_terms: D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use; D003315:Cornea / Q000187:drug effects; D005126:Eye Burns / Q000139:chemically induced / Q000188:drug therapy*; D051034:Inhibitor of Apoptosis Proteins / Q000737:chemistry / Q000627:therapeutic use*; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D007781:Lactoferrin / Q000737:chemistry / Q000627:therapeutic use*; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012972:Sodium Hydroxide
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012972:Sodium Hydroxide; D048271:Chitosan; D007781:Lactoferrin
keywords: Bovine lactoferrin; Chitosan; Corneal irritation; PLGA; SurR9-C84A