title: Comparative cytotoxicity of dolomite nanoparticles in human larynx HEp2 and liver HepG2 cells.
abstract: Dolomite is a natural mineral of great industrial and commercial importance. With the advent of nanotechnology, natural minerals including dolomite in the form of nanoparticles (NPs) are being utilized in various applications to improve the quality of products. However, safety or toxicity information of dolomite NPs is largely lacking. This study evaluated the cytotoxicity of dolomite NPs in two widely used in vitro cell culture models: human airway epithelial (HEp2) and human liver (HepG2) cells. Concentration-dependent decreased cell viability and damaged cell membrane integrity revealed the cytotoxicity of dolomite NPs. We further observed that dolomite NPs induce oxidative stress in a concentration-dependent manner, as indicated by depletion of glutathione and induction of reactive oxygen species (ROS) and lipid peroxidation. Quantitative real-time PCR data demonstrated that the mRNA level of tumor suppressor gene p53 and apoptotic genes (bax, CASP3 and CASP9) were up-regulated whereas the anti-apoptotic gene bcl-2 was down-regulated in HEp2 and HepG2 cells exposed to dolomite NPs. Moreover, the activity of apoptotic enzymes (caspase-3 and caspase-9) was also higher in both kinds of cells treated with dolomite NPs. It is also worth mentioning that HEp2 cells seem to be marginally more susceptible to dolomite NPs exposure than HepG2 cells. Cytotoxicity induced by dolomite NPs was efficiently prevented by N-acetyl cysteine treatment, which suggests that oxidative stress is primarily responsible for the cytotoxicity of dolomite NPs in both HEp2 and HepG2 cells. Toxicity mechanisms of dolomite NPs warrant further investigations at the in vivo level.
authors: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, 11451, Saudi Arabia.
authors: Maqusood
authors: M
authors: Ahamed
authors: Hisham A
authors: HA
authors: Alhadlaq
authors: Javed
authors: J
authors: Ahmad
authors: Maqsood A
authors: MA
authors: Siddiqui
authors: Shams T
authors: ST
authors: Khan
authors: Javed
authors: J
authors: Musarrat
authors: Abdulaziz A
authors: AA
authors: Al-Khedhairy
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002119:Calcium Carbonate / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000032:analysis; D056945:Hep G2 Cells / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D007820:Laryngeal Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D008274:Magnesium / Q000633:toxicity*; D053768:Metal Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; D053453:Caspase 9; D005978:Glutathione; D002119:Calcium Carbonate; D008274:Magnesium; C028042:calcium magnesium carbonate
keywords: apoptosis; cytotoxicity; dolomite nanoparticles; human health; oxidative stress