title: The effect of systemic amantadine sulfate on malondialdehyde and total thiol levels in rat corneas.
abstract: OBJECTIVE
  To evaluate the malondialdehyde (MDA) and total thiol (sulfhydryl, SH) levels in rat corneas after intraperitoneal injection of amantadine sulfate.
  
  
  METHODS
  A total of 12 Wistar albino rats were divided into two groups: control group (n = 6) and amantadine group (n = 6). Balanced salt solution (1 mL, 0.9% NaCl, twice/day) was injected into rats in control group. Amantadine sulfate (2 mg/1 mL, twice/day) was injected into rats in amantadine group. In each group, two rats were injected for 1 week, two received injections for 1 month, and two rats received injections for 3 months. The corneas were homogenized and MDA and SH levels were measured spectroflourometrically.
  
  
  RESULTS
  In control group, median MDA and SH levels were 2.37 (range, 0.92-3.60) and 25.35 (range, 6.30-54.0) nmol/mg, respectively. In amantadine group, median MDA and SH levels were 3.57 (range, 1.25-5.92) and 32.65 (range, 3.30-48.3) nmol/mg, respectively. The difference between this two groups regarding MDA (P = 0.14) and SH (P = 1.0) levels was statistically insignificant.
  
  
  CONCLUSIONS
  Systemically administered amantadine sulfate seems not to cause MDA and SH imbalance in rat corneas.
authors: Department of Ophthalmology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey.
authors: Züleyha
authors: Z
authors: Yalniz-Akkaya
authors: Department of Biochemistry, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey.
authors: Vildan
authors: V
authors: Fidanci
authors: Department of Biochemistry, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey.
authors: Aytul
authors: A
authors: Kilinc
authors: Department of Ophthalmology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey.
authors: Ayse
authors: A
authors: Burcu
authors: Department of Ophthalmology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey.
authors: Guner O
authors: GO
authors: Uney
authors: Department of Ophthalmology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey.
authors: Firdevs
authors: F
authors: Ornek
keywords: Amantadin Sulfate; Anti-Oxidant; Cornea; Malondyaldehide; Oxidant; Rats; Total Thiol