title: Transforming growth factor-β inhibits IQ motif containing guanosine triphosphatase activating protein 1 expression in lung fibroblasts via the nuclear factor-κB signaling pathway.
abstract: IQ motif containing guanosine triphosphatase activating protein 1 (IQGAP1) is associated with idiopathic pulmonary fibrogenesis (IPF); however, characterization of the expression of IQGAP1 in lung fibroblasts has remained elusive. The present study therefore evaluated IQGAP1 expression in mouse and human lung fibroblasts under fibrotic conditions via western blot analysis. It was revealed that IQGAP1 expression levels were significantly decreased in lung fibroblasts isolated from bleomycin-challenged mice than in those of control mice. Transforming growth factor-β (TGF-β) induced differentiation, as well as decreased expression of IQGAP1 in WI-38 cells human lung fibroblasts. Furthermore, inhibition of nuclear factor (NF)-κB activation restored the TGF-β-induced inhibition of IQGAP1 expression in WI-38 cells. In lysophosphatidic acid (LPA)-challenged WI-38 cells, the expression of IQGAP1 was also decreased, while neutralized anti-TGF-β antibody treatment restored the LPA-induced inhibition of IQGAP1 expression. These data indicated that TGF-β inhibited IQGAP1 expression in lung fibroblasts via the NF-κB signaling pathway, presenting a potential novel therapeutic target for the treatment of IPF.
authors: Department of Anesthesiology, Huai'an Hospital Affiliated to Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an, Jiangsu 223002, P.R. China.
authors: Chuanyue
authors: C
authors: Zong
authors: Department of Anesthesiology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.
authors: Xianlong
authors: X
authors: Zhang
authors: Department of Anesthesiology, Huai'an Hospital Affiliated to Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an, Jiangsu 223002, P.R. China.
authors: Ying
authors: Y
authors: Xie
authors: Department of Anesthesiology, The First People's Hospital of Suqian, Suqian, Jiangsu 223800, P.R. China.
authors: Jiawen
authors: J
authors: Cheng
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000008:administration & dosage; D002454:Cell Differentiation / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000235:genetics* / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008246:Lysophospholipids / Q000008:administration & dosage; D051379:Mice; D016328:NF-kappa B / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D020703:ras GTPase-Activating Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C101203:IQ motif containing GTPase activating protein 1; D008246:Lysophospholipids; D016328:NF-kappa B; D016212:Transforming Growth Factor beta; D020703:ras GTPase-Activating Proteins; D001761:Bleomycin; C032881:lysophosphatidic acid