title: Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs).
abstract: The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.
authors: MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DS, UK.
authors: Lucas V
authors: LV
authors: Greder
authors: Stem Cell Institute, University of Minnesota, 2-220 MTRF, 2001 6th Street SE, Minneapolis, MN, 55455, USA.
authors: Jason
authors: J
authors: Post
authors: Stem Cell Institute, University of Minnesota, 2-220 MTRF, 2001 6th Street SE, Minneapolis, MN, 55455, USA. dutto015@umn.edu.
authors: James R
authors: JR
authors: Dutton
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D019070:Cell Lineage; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D019426:Integrases; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D010368:Pectins; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000502:physiology; D013629:Tamoxifen / Q000494:pharmacology; D019076:Transgenes
chemical_list: D020413:3' Untranslated Regions; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D008164:Luminescent Proteins; C489428:Myc protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C485184:enhanced green fluorescent protein; C413663:fluorescent protein 583; C043411:proteinase inhibitor-inducing factor (tomato); D013629:Tamoxifen; D049452:Green Fluorescent Proteins; D010368:Pectins; C045073:Cre recombinase; D019426:Integrases
keywords: Induced pluripotent stem cell; Lineage tracing; Oct4; Oct4-Cre; Reprogramming; iPSC