title: Pirin regulates epithelial to mesenchymal transition independently of Bcl3-Slug signaling.
abstract: Epithelial to mesenchymal transition (EMT) is an important mechanism for the initial step of metastasis. Proteomic analysis indicates that Pirin is involved in metastasis. However, there are no reports demonstrating its direct contribution. Here we investigated the involvement of Pirin in EMT. In HeLa cells, Pirin suppressed E-cadherin expression and regulated the expression of other EMT markers. Furthermore, cells expressing Pirin exhibited a spindle-like morphology, which is reminiscent of EMT. A Pirin mutant defective for Bcl3 binding decreased E-cadherin expression similar to wild-type, suggesting that Pirin regulates E-cadherin independently of Bcl3-Slug signaling. These data provide direct evidence that Pirin contributes to cancer metastasis.
authors: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors: Kuniya
authors: K
authors: Komai
authors: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors: Yuki
authors: Y
authors: Niwa
authors: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors: Yukiko
authors: Y
authors: Sasazawa
authors: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. Electronic address: simizu@applc.keio.ac.jp.
authors: Siro
authors: S
authors: Simizu
mesh_terms: D015703:Antigens, CD; D000081242:B-Cell Lymphoma 3 Protein; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000502:physiology*; D002465:Cell Movement; D048430:Cell Shape; D049308:Dioxygenases; D058750:Epithelial-Mesenchymal Transition; D015870:Gene Expression; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000081242:B-Cell Lymphoma 3 Protein; C000631344:BCL3 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D002352:Carrier Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D049308:Dioxygenases; C105340:PIR protein, human
keywords: E-cadherin; Epithelial to mesenchymal transition; Metastasis; Morphological change; Pirin