title: b-Series gangliosides crucially regulate leptin secretion in adipose tissues.
abstract: Gangliosides are widely involved in the regulation of cells and organs. However, little is known about their roles in leptin secretion from adipose tissues. Genetic deletion of b-series gangliosides resulted in the marked reduction of serum leptin. Expression analysis of leptin revealed that leptin accumulated in the adipose tissues of GD3 synthase-knockout (GD3S KO) mice. Analysis of primary cultured stromal vascular fractions (SVF) derived from GD3S KO mice revealed that leptin secretion was reduced, although leptin amounts in cells were increased compared with those of wild type. Interestingly, addition of b-series gangliosides to the culture medium of differentiated SVF resulted in the restoration of leptin secretion. Results of methyl-β-cyclodextrin treatment of differentiated 3T3-L1 cells as well as immunocytostaining of leptin and caveolin-1 suggested that b-series gangliosides regulate the leptin secretion from adipose tissues in lipid rafts.
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Shuting
authors: S
authors: Ji
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Yuki
authors: Y
authors: Ohkawa
authors: Department of Functional Anatomy and Neuroscience, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Kyohei
authors: K
authors: Tokizane
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Yuhsuke
authors: Y
authors: Ohmi
authors: Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Ryoichi
authors: R
authors: Banno
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan; Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai 487-8501, Japan.
authors: Keiko
authors: K
authors: Furukawa
authors: Department of Functional Anatomy and Neuroscience, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan.
authors: Hiroshi
authors: H
authors: Kiyama
authors: Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan. Electronic address: koichi@med.nagoya-u.ac.jp.
authors: Koichi
authors: K
authors: Furukawa
mesh_terms: D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism; D002454:Cell Differentiation; D005732:Gangliosides / Q000378:metabolism*; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D012799:Sialyltransferases / Q000172:deficiency / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D051242:Caveolin 1; D005732:Gangliosides; D020738:Leptin; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase
keywords: Adipose tissue; Ganglioside; Leptin; Lipid raft