title: A triple-arginine motif in the amino-terminal domain and oligomerization are required for HIV-1 inhibition by human MX2.
abstract: We have employed molecular genetic approaches to understand the domain organization of the HIV-1 resistance factor myxovirus resistance 2 (MX2). First, we describe an essential triple-arginine motif in the amino-terminal domain. Second, we demonstrate that this 91-residue domain mediates antiviral activity when appended to heterologous proteins, and we provide genetic evidence that protein oligomerization is required for MX2 function. These insights will facilitate future work aiming to elucidate MX2's mechanism of action.
authors: Department of Infectious Diseases, King's College London, London, United Kingdom cgoujon@cpbs.cnrs.fr michael.malim@kcl.ac.uk.
authors: Caroline
authors: C
authors: Goujon
authors: Department of Infectious Diseases, King's College London, London, United Kingdom.
authors: Rebecca A
authors: RA
authors: Greenbury
authors: Department of Infectious Diseases, King's College London, London, United Kingdom.
authors: Stelios
authors: S
authors: Papaioannou
authors: Department of Infectious Diseases, King's College London, London, United Kingdom.
authors: Tomas
authors: T
authors: Doyle
authors: Department of Infectious Diseases, King's College London, London, United Kingdom cgoujon@cpbs.cnrs.fr michael.malim@kcl.ac.uk.
authors: Michael H
authors: MH
authors: Malim
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics*; D001120:Arginine / Q000235:genetics; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins / Q000235:genetics* / Q000378:metabolism*; D058105:Polymerization
chemical_list: C579068:MX2 protein, human; D064547:Myxovirus Resistance Proteins; D001120:Arginine