title: Cloning of genomic sequences of three crustacean hyperglycemic hormone superfamily genes and elucidation of their roles of regulating insulin-like androgenic gland hormone gene.
abstract: The insulin-like androgenic gland hormone (IAG) gene in crustaceans plays an important role in male sexual differentiation, metabolism, and growth. However, the upstream regulation of IAG signaling schemes remains poorly studied. In the present study, we cloned the 5' flanking sequence of IAG and full-length genomic sequences of gonad-inhibiting hormone (Mn-GIH), molt-inhibiting hormone (Mn-MIH) and crustacean hyperglycemic hormone (Mn-CHH) in Macrobrachium nipponense. We identified the transcription factor-binding sites in the 5' flanking sequence of IAG and investigated the exon-intron patterns of the three CHH superfamily genes. Each CHH superfamily gene consisted of two introns separating three exons. Mn-GIH and Mn-MIH shared the same intron insertion sites, which differed from Mn-CHH. We provided DNA-level evidence for the type definition. We also identified two cAMP response elements in the 5' untranslated region. We further investigated the regulatory relationships between Mn-GIH, Mn-MIH, and Mn-CHH and IAG at the transcriptional level by injection of double-stranded RNA (dsRNA). IAG transcription levels were significantly increased to 660.2%, 472.9%, and 112.4% of control levels in the Mn-GIH dsRNA, Mn-MIH dsRNA, and Mn-CHH dsRNA groups, respectively. The results clearly demonstrated that Mn-GIH and Mn-MIH, but not Mn-CHH, negatively regulate the expression of the IAG gene.
authors: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China; Weifang University of Science and Technology, Shouguang 262700, PR China.
authors: Fajun
authors: F
authors: Li
authors: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China.
authors: Hongkun
authors: H
authors: Bai
authors: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors: Wenyi
authors: W
authors: Zhang
authors: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China. Electronic address: fuht@ffrc.cn.
authors: Hongtuo
authors: H
authors: Fu
authors: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China.
authors: Fengwei
authors: F
authors: Jiang
authors: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China.
authors: Guoxia
authors: G
authors: Liang
authors: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors: Shubo
authors: S
authors: Jin
authors: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors: Shengming
authors: S
authors: Sun
authors: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors: Hui
authors: H
authors: Qiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics; D005123:Eye / Q000166:cytology; D005786:Gene Expression Regulation; D042341:Gonadal Hormones / Q000235:genetics*; D007447:Invertebrate Hormones / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D033541:Palaemonidae / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D034622:RNA Interference; D034741:RNA, Small Interfering; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012733:Sex Differentiation / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D060829:Arthropod Proteins; D002352:Carrier Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D042341:Gonadal Hormones; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C053644:androgenic gland hormone (Armadillidium); C015947:hyperglycemic hormone, crustacean; C012445:molt-inhibiting hormone; C073189:vitellogenesis inhibiting hormone
keywords: Crustacean hyperglycemic hormone; Gonad-inhibiting hormone; Insulin-like androgenic gland hormone gene; Macrobrachium nipponense; Molt-inhibiting hormone