title: Study of DNA adsorption on mica surfaces using a surface force apparatus.
abstract: We report our studies on the adsorption properties of double-stranded DNA molecules on mica surfaces in a confined environment using a surface force apparatus. Specifically, we studied the influence of cation species and concentrations on DNA adsorption properties. Our results indicated that divalent cations (Mg(2+) and Co(2+)) preferred to form uniform and moderately dense DNA layers on a mica substrate. By measuring the interactions between DNA-coated mica and bare mica in an aqueous solution, obvious adhesion was observed in a cobalt chloride solution, possibly due to the ion-correlation attraction between negatively charged DNA and the mica surface. Furthermore, the interaction differences that were observed with MgCl2 and CoCl2 solutions reveal that the specific adsorption behaviors of DNA molecules on a mica substrate were mediated by these two salts. Our results are helpful to elucidate the dynamics of DNA binding on a solid substrate.
authors: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, and School of Mechanical Engineering, Southeast University, Nanjing 211189, China.
authors: Yajing
authors: Y
authors: Kan
authors: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, and School of Mechanical Engineering, Southeast University, Nanjing 211189, China.
authors: Qiyan
authors: Q
authors: Tan
authors: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, and School of Mechanical Engineering, Southeast University, Nanjing 211189, China.
authors: Gensheng
authors: G
authors: Wu
authors: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, and School of Mechanical Engineering, Southeast University, Nanjing 211189, China.
authors: Wei
authors: W
authors: Si
authors: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, and School of Mechanical Engineering, Southeast University, Nanjing 211189, China.
authors: Yunfei
authors: Y
authors: Chen
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015636:Magnesium Chloride / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D002412:Cations; D015636:Magnesium Chloride; D003035:Cobalt; D004247:DNA; C018021:cobaltous chloride; C011934:mica