title: Phylogenetic relationships and morphological evolution in Lentinus, Polyporellus and Neofavolus, emphasizing southeastern Asian taxa.
abstract: The genus Lentinus (Polyporaceae, Basidiomycota) is widely documented from tropical and temperate forests and is taxonomically controversial. Here we studied the relationships between Lentinus subg. Lentinus sensu Pegler (i.e. sections Lentinus, Tigrini, Dicholamellatae, Rigidi, Lentodiellum and Pleuroti and polypores that share similar morphological characters). We generated sequences of internal transcribed spacers (ITS) and partial 28S regions of nuc rDNA and genes encoding the largest subunit of RNA polymerase II (RPB1), focusing on Lentinus subg. Lentinus sensu Pegler and the Neofavolus group, combined these data with sequences from GenBank (including RPB2 gene sequences) and performed phylogenetic analyses with maximum likelihood and Bayesian methods. We also evaluated the transition in hymenophore morphology between Lentinus, Neofavolus and related polypores with ancestral state reconstruction. Single-gene phylogenies and phylogenies combining ITS and 28S with RPB1 and RPB2 genes all support existence of a Lentinus/Polyporellus clade and a separate Neofavolus clade. Polyporellus (represented by P. arcularius, P. ciliatus, P. brumalis) forms a clade with species representing Lentinus subg. Lentinus sensu Pegler (1983), excluding L. suavissimus. Lentinus tigrinus appears as the sister group of Polyporellus in the four-gene phylogeny, but this placement was weakly supported. All three multigene analyses and the single-gene analysis using ITS strongly supported Polyporus tricholoma as the sister group of the Lentinus/Polyporellus clade; only the 28S rRNA phylogeny failed to support this placement. Under parsimony the ancestral hymenophoral configuration for the Lentinus/Polyporellus clade is estimated to be circular pores, with independent transitions to angular pores and lamellae. The ancestral state for the Neofavolus clade is estimated to be angular pores, with a single transition to lamellae in L. suavissimus. We propose that Lentinus suavissimus (section Pleuroti) should be reclassified as Neofavolus suavissimus comb. nov.
authors: Biology Department, Clark University, 950 Main Street, Worcester, Massachusetts 01610, Institute for Tropical Biology and Conservation (ITBC), Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.
authors: Jaya Seelan Sathiya
authors: JS
authors: Seelan
authors: Biology Department, Clark University, 950 Main Street, Worcester, Massachusetts 01610.
authors: Alfredo
authors: A
authors: Justo
authors: Biology Department, Clark University, 950 Main Street, Worcester, Massachusetts 01610.
authors: Laszlo G
authors: LG
authors: Nagy
authors: Mahidol University International College (Science Division), 999 Phuttamonthon, Sai 4, Salaya, Nakorn Pathom 73170, Thailand.
authors: Edward A
authors: EA
authors: Grand
authors: ECORC, Science & Technology Branch, Agriculture & Agri-Food Canada, CEF, Neatby Building, Ottawa, Ontario, K1A 0C6 Canada.
authors: Scott A
authors: SA
authors: Redhead
authors: Biology Department, Clark University, 950 Main Street Worcester, Massachusetts 01610 dhibbett@clarku.edu.
authors: David
authors: D
authors: Hibbett
mesh_terms: D001208:Asia; D004275:DNA, Ribosomal / Q000235:genetics; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics; D020074:Lentinula / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D011124:Polyporaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012319:RNA Polymerase II / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D005656:Fungal Proteins; D012335:RNA, Ribosomal; D012319:RNA Polymerase II
keywords: Lentinus sensu stricto; Lentinus suavissimus; PolyPEET; multigene phylogeny; taxonomy