title: Forced-flow planar chromatography in the rear view mirror.
abstract: Mobile phase progress in planar stationary phase can be evoked by either external or internal forces. An internal force is capillarity, while gravity, electric field, a pump and centrifugal forces belong to external forces. Overpressured layer chromatography gives a widely used special chapter of forced-flow planar chromatography, a special bridge between high-performance liquid column chromatography and thin-layer chromatography (TLC). A simple and special rule characterizes the progress of mobile phase. Optimal efficiency is composed by the doubled effect of flow resulting from the pump-forced mobile phase (convex profile of laminar flow) and capillary forces on the dry stationary phase (concave laminar flow). This review describes the most important aspects of forced-flow TLC, including how the set-ups are developed and also the progress of detection methods used.
authors: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary drkalasz@gmail.com.
authors: Huba
authors: H
authors: Kalász
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids