title: β Integrin-like protein-mediated adhesion and its disturbances during cell cultivation of the mussel Mytilus trossulus.
abstract: In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell-substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp-Ser (RGDS)-peptide, the Ca(2+)/Mg(2+)-chelators and the stress influence of freezing-thawing, reveals that all these factors resulted in the partial destruction of the integrin-extracellular matrix (ECM) interaction in culture and, in particular, changes in the distribution and relative abundance of β integrin-positive cells. The experiments, carried out on selected substrates, found that β integrin-positive cells demonstrate different affinities for the substrates. This finding further supports the assumption that epithelial differentiation in cultivated cells of larval Mytilus may be mediated by β integrin-like proteins via binding to laminin; direct binding to other components of the ECM could not be demonstrated. The mussel β integrin-positive cells are not involved in myogenic or neuronal differentiation on any of the substrates but part of them has tubulin-positive cilia, forming some epithelia-like structures. Our data indicate that β integrin-positive cells are able to proliferate in vitro which suggests that they could participate in renewing the digestive epithelium in larvae. The findings provide evidence that the distribution pattern of β integrin-like protein depends on the cell type and the factors influencing the adhesion.
authors: A. V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevsky Str. 17, 690041, Vladivostok, Russia.
authors: Mariia A
authors: MA
authors: Maiorova
authors: Nelly A
authors: NA
authors: Odintsova
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D015925:Cryopreservation; D039641:Integrin beta Chains / Q000032:analysis* / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D049878:Mytilus / Q000166:cytology* / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism
chemical_list: D039641:Integrin beta Chains; D009842:Oligopeptides; C046617:arginyl-glycyl-aspartyl-serine; D008274:Magnesium; D002118:Calcium