title: The involvement of FAK-PI3K-AKT-Rac1 pathway in porcine reproductive and respiratory syndrome virus entry.
abstract: CD163 and sialoadhesin had been reported as the two receptors for porcine reproductive and respiratory syndrome virus (PRRSV) infection. The signaling pathway activated by PRRSV entry was seldom reported. In our studies, we demonstrated that PRRSV entry triggers FAK, PI3K, AKT and Rac1 activation. The signaling pathway FAK-PI3K-AKT-Rac1 is essential for PRRSV entry. Blocking FAK by PF573228 attenuates the activation of PI3K, AKT, Rac1 and the cytoskeleton remodeling induced by virus entry. Inhibitors to FAK, PI3K, AKT and Rac1 can significantly inhibit the virus entry. In conclusion, our observations reveal that PRRSV triggers the activation of FAK-PI3K-AKT-Rac1 signaling pathway to facilitate its entry into cells.
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Bo
authors: B
authors: Ni
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Science, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China.
authors: Li-Bin
authors: LB
authors: Wen
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Rui
authors: R
authors: Wang
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Hong-Ping
authors: HP
authors: Hao
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Chang-Chao
authors: CC
authors: Huan
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Xin
authors: X
authors: Wang
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Li
authors: L
authors: Huang
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
authors: Jin-Feng
authors: JF
authors: Miao
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal, Infectious Diseases and Zoonoses, Yangzhou, 225009, China. Electronic address: fhj@njau.edu.cn.
authors: Hong-Jie
authors: HJ
authors: Fan
authors: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China. Electronic address: xmao@njau.edu.cn.
authors: Xiang
authors: X
authors: Mao
mesh_terms: D000818:Animals; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000201:enzymology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D015398:Signal Transduction; D013552:Swine / Q000378:metabolism* / Q000821:virology*; D053586:Virus Internalization; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D010770:Phosphotransferases; D019869:Phosphatidylinositol 3-Kinases; D051417:Focal Adhesion Kinase 1; D051058:Oncogene Protein v-akt; D020830:rac1 GTP-Binding Protein
keywords: FAK; Infection; Porcine reproductive and respiratory syndrome virus; Rac1