title: Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria.
abstract: The bactericidal effect of various types of photoirradiated polyphenols against Gram-positive and -negative bacteria was evaluated in relation to the mode of action. Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, and Streptococcus mutans) and Gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, and Pseudomonas aeruginosa) suspended in a 1 mg/mL polyphenol aqueous solution (caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigallocatechin gallate, and proanthocyanidin) were exposed to LED light (wavelength, 400 nm; irradiance, 260 mW/cm(2)) for 5 or 10 min. Caffeic acid and chlorogenic acid exerted the highest bactericidal activity followed by gallic acid and proanthocyanidin against both Gram-positive and -negative bacteria. It was also demonstrated that the disinfection treatment induced oxidative damage of bacterial DNA, which suggests that polyphenols are incorporated into bacterial cells. The present study suggests that blue light irradiation of polyphenols could be a novel disinfection treatment.
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Keisuke
authors: K
authors: Nakamura
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Kirika
authors: K
authors: Ishiyama
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Hong
authors: H
authors: Sheng
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Hiroyo
authors: H
authors: Ikai
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Taro
authors: T
authors: Kanno
authors: Laboratory for Redox Regulation, ‡Division of Molecular and Regenerative Prosthodontics, and #Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry , 4-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors: Yoshimi
authors: Y
authors: Niwano
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D008027:Light; D008826:Microbial Sensitivity Tests; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D059808:Polyphenols
keywords: Gram-negative bacteria; Gram-positive bacteria; bactericidal effect; photoirradiation; polyphenols