title: Effect of curcumin caged silver nanoparticle on collagen stabilization for biomedical applications.
abstract: The current study aims at understanding the influence of curcumin caged silver nanoparticle (CCSNP) on stability of collagen. The results indicated that curcumin caged silver nanoparticles efficiently stabilize collagen, indicated by enhanced tensile strength, fibril formation and viscosity. The tensile strength of curcumin caged silver nanoparticle cross-linked collagen and elongation at break was also found to be higher than glutaraldehyde cross-linked collagen. The physicochemical characteristics of curcumin caged nanoparticle cross-linked collagen exhibited enhanced strength. The thermal properties were also good with both thermal degradation temperature and hydrothermal stability higher than native collagen. CD analysis showed no structural disparity in spite of superior physicochemical properties suggesting the significance of curcumin caged nanoparticle mediated cross-linking. The additional enhancement in the stabilization of collagen could be attributed to multiple sites for interaction with collagen molecule provided by curcumin caged silver nanoparticles. The results of cell proliferation and anti-microbial activity assays indicated that curcumin caged silver nanoparticles promoted cell proliferation and inhibited microbial growth making it an excellent biomaterial for wound dressing application. The study opens scope for nano-biotechnological strategies for the development of alternate non-toxic cross-linking agents facilitating multiple site interaction thereby improving therapeutic values to the collagen for biomedical application.
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
authors: Kunnavakkam Vinjimur
authors: KV
authors: Srivatsan
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors: N
authors: N
authors: Duraipandy
authors: University of Madras, Chennai, India.
authors: Shajitha
authors: S
authors: Begum
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
authors: Rachita
authors: R
authors: Lakra
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India.
authors: Usha
authors: U
authors: Ramamurthy
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors: Purna Sai
authors: PS
authors: Korrapati
authors: Council of Scientific and Industrial Research, Central Leather Research Institute, Adyar, Chennai 600020, India; Academy of Scientific and Innovative Research, New Delhi, India. Electronic address: kiranmsk112@gmail.com.
authors: Manikantan Syamala
authors: MS
authors: Kiran
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D020811:Biomedical Technology / Q000379:methods*; D002152:Calorimetry, Differential Scanning; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D003094:Collagen / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D000067493:Dynamic Light Scattering; D006801:Humans; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011208:Powders; D051381:Rats; D012212:Rheology; D012834:Silver / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013718:Tensile Strength; D013818:Thermogravimetry; D014783:Viscosity; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D011208:Powders; D012834:Silver; D003094:Collagen; D003474:Curcumin
keywords: Anti-microbial; Biocompatible; Collagen; Curcumin; Silver nanoparticles; Stabilization