title: [The kinetic parameters of retainment and release of deposited in human tissue structures calcium].
abstract: The analysis of retainment and release kinetics of deposited in tissue structures calcium was made in the hypercalcemic conditions in 28 healthy volunteers (22 males and 6 females) of the age of 33 ± 6.5 years via drip infusion (Groups 1, 2) and in 9 individuals (3 males and 6 females) in 12 trials via stream infusion (Group 3). By the end of each hour after the termination of calcium infusion the amount of calcium retained in tissues was calculated (Mtis./kg); the parameters of its binding (specific buffer volume--β3 sp, association constant--Ka, number of binding centers--n) were established using the Langmuir and Scetchard coordinates. The Group 1 volunteers (n = = 12) showed a section of positive cooperativity (a direct regression on Sketchard coordinates, Hill coefficient 3.36 ± 1.63) and 2 sections of the consecutive calcium separation from one set of noninteracting centers. 5 volunteers of Group 2 and 8 volunteers of Group 3 demonstrated a slight calcium delivery to tissues after 1 hour of observation which then followed for 2 volunteers of Group 2 and for 2 volunteers of Group 3. Other volunteers of Groups 2 and 3 showed a release of tissue-deposited calcium via the mechanism of the consecutive separation from one set of noninteracting centers with βsp 3 times less and Ka 7 times higher than with the calcium infusion. The excretion of calcium in urine was the highest in Group 1 and the lowest in Group 3. The [Ca2+] and Mtis./kg values were normalized in Groups 1 and 2 the next morning and in Group 3 after 2-3 hours of observation. An assumption was made about the relationship between the tissue and kidney [Ca2+] normalizing mechanisms with hypercalcemia.
authors: I P
authors: IP
authors: Ermakova
authors: T V
authors: TV
authors: Potanina
authors: I A
authors: IA
authors: Pronchenko
authors: I M
authors: IM
authors: Larina
authors: V I
authors: VI
authors: Sevast'ianov
mesh_terms: D000328:Adult; D002118:Calcium / Q000097:blood / Q000652:urine; D002125:Calcium Gluconate / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D006934:Hypercalcemia / Q000097:blood* / Q000139:chemically induced / Q000652:urine*; D007262:Infusions, Intravenous; D007668:Kidney / Q000378:metabolism*; D008297:Male
chemical_list: D002125:Calcium Gluconate; D002118:Calcium