title: Role of histamine H3 receptor in glucagon-secreting αTC1.6 cells.
abstract: Pancreatic α-cells secrete glucagon to maintain energy homeostasis. Although histamine has an important role in energy homeostasis, the expression and function of histamine receptors in pancreatic α-cells remains unknown. We found that the histamine H3 receptor (H3R) was expressed in mouse pancreatic α-cells and αTC1.6 cells, a mouse pancreatic α-cell line. H3R inhibited glucagon secretion from αTC1.6 cells by inhibiting an increase in intracellular Ca(2+) concentration. We also found that immepip, a selective H3R agonist, decreased serum glucagon concentration in rats. These results suggest that H3R modulates glucagon secretion from pancreatic α-cells.
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Tadaho
authors: T
authors: Nakamura
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Takeo
authors: T
authors: Yoshikawa
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Fumito
authors: F
authors: Naganuma
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Attayeb
authors: A
authors: Mohsen
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Tomomitsu
authors: T
authors: Iida
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Yamato
authors: Y
authors: Miura
authors: Department of Molecular Endocrinology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Akira
authors: A
authors: Sugawara
authors: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors: Kazuhiko
authors: K
authors: Yanai
keywords: CNS, central nervous system; GLP, glucagon-like peptide; Glucagon secretion; H3KO, histamine H3 receptor-gene knockout; H3R, histamine H3 receptor; Histamine H3 receptor; KRB, Krebs–Ringer bicarbonate buffer; Pancreatic α-cells; VDCCs, voltage-dependent Ca2+ channels; αTC1.6 cells