title: Tight junction proteins in the canine epidermis: a pilot study on their distribution in normal and in high IgE-producing canines.
abstract: Epidermal tight junctions (TJ) have been well-described in human medicine and are involved in many skin diseases such as atopic dermatitis (AD). In dogs, there are no data regarding the implication of TJ in skin diseases including canine AD. The aim of this study was to compare the expression and the distribution of ZO-1, occludin and claudin-1 in the epidermis of healthy and atopic dogs. Skin biopsies from 6 high IgE-producing beagles sensitized to house dust mite (atopic group) were used. Skin specimens from nine healthy dogs without skin issues were sampled (healthy group). Immunoperoxydase staining was used to study the staining pattern of zonula occludens-1 (ZO-1), occludin and claudin-1 in the epidermis of healthy and atopic dogs. Positive controls were healthy human skin samples. Labeling patterns were assessed by 2 examiners blinded to the identities of the specimens. Comparisons between groups were performed using an exact Wilcoxon-Mann-Whitney test. The mean total expression score of claudin-1 was lower in atopic dogs as compared to healthy subjects. Occludin and ZO-1 expression remained unchanged within each group. These results suggest a defect in claudin-1 expression in the nonlesional epidermis of atopic dogs.
authors: ONIRIS, Departments of Dermatology, Parasitology, Mycology, Atlanpole - La Chantrerie, Route de Gachet, Nantes, F-44307, France (Roussel, Bruet, Bourdeau); Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2015 SW 16th Avenue, University of Florida, Gainesville, Florida 32610, USA (Marsella); and CRCNA - UMR 892 - INSERM/6299 CNRS/Université de Nantes, Laboratoire de dermatoimmunologie, RJ PT1, Institut de Biologie, CHU Hôtel Dieu, 9 quai Moncousu, Nantes, F-44093, France (Knol).
authors: Anne J J
authors: AJ
authors: Roussel
authors: ONIRIS, Departments of Dermatology, Parasitology, Mycology, Atlanpole - La Chantrerie, Route de Gachet, Nantes, F-44307, France (Roussel, Bruet, Bourdeau); Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2015 SW 16th Avenue, University of Florida, Gainesville, Florida 32610, USA (Marsella); and CRCNA - UMR 892 - INSERM/6299 CNRS/Université de Nantes, Laboratoire de dermatoimmunologie, RJ PT1, Institut de Biologie, CHU Hôtel Dieu, 9 quai Moncousu, Nantes, F-44093, France (Knol).
authors: Vincent
authors: V
authors: Bruet
authors: ONIRIS, Departments of Dermatology, Parasitology, Mycology, Atlanpole - La Chantrerie, Route de Gachet, Nantes, F-44307, France (Roussel, Bruet, Bourdeau); Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2015 SW 16th Avenue, University of Florida, Gainesville, Florida 32610, USA (Marsella); and CRCNA - UMR 892 - INSERM/6299 CNRS/Université de Nantes, Laboratoire de dermatoimmunologie, RJ PT1, Institut de Biologie, CHU Hôtel Dieu, 9 quai Moncousu, Nantes, F-44093, France (Knol).
authors: Rosanna
authors: R
authors: Marsella
authors: ONIRIS, Departments of Dermatology, Parasitology, Mycology, Atlanpole - La Chantrerie, Route de Gachet, Nantes, F-44307, France (Roussel, Bruet, Bourdeau); Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2015 SW 16th Avenue, University of Florida, Gainesville, Florida 32610, USA (Marsella); and CRCNA - UMR 892 - INSERM/6299 CNRS/Université de Nantes, Laboratoire de dermatoimmunologie, RJ PT1, Institut de Biologie, CHU Hôtel Dieu, 9 quai Moncousu, Nantes, F-44093, France (Knol).
authors: Anne Chantal
authors: AC
authors: Knol
authors: ONIRIS, Departments of Dermatology, Parasitology, Mycology, Atlanpole - La Chantrerie, Route de Gachet, Nantes, F-44307, France (Roussel, Bruet, Bourdeau); Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 2015 SW 16th Avenue, University of Florida, Gainesville, Florida 32610, USA (Marsella); and CRCNA - UMR 892 - INSERM/6299 CNRS/Université de Nantes, Laboratoire de dermatoimmunologie, RJ PT1, Institut de Biologie, CHU Hôtel Dieu, 9 quai Moncousu, Nantes, F-44093, France (Knol).
authors: Patrick J
authors: PJ
authors: Bourdeau
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D062445:Claudin-1 / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology / Q000378:metabolism*; D004285:Dogs; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007150:Immunohistochemistry; D062793:Occludin / Q000378:metabolism; D010865:Pilot Projects; D016037:Single-Blind Method; D062725:Tight Junction Proteins / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D015415:Biomarkers; D062445:Claudin-1; D062793:Occludin; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein; D007073:Immunoglobulin E