title: Interleukin-4 affects the mature phenotype and function of rat bone marrow-derived dendritic cells.
abstract: Granulocyte macrophage‑colony stimulating factor (GM‑CSF), and GM‑CSF plus interleukin‑4 (GM‑CSF + IL‑4) are two commonly‑used cytokine therapies for the generation of bone marrow‑derived dendritic cells (DCs). However, the mechanisms underlying IL‑4 involvement in DC generation and maturation remain unclear. In order to investigate the effect of IL‑4 on DC generation, DCs from rat bone marrow progenitors were generated using GM‑CSF, with and without IL‑4. GM‑CSF + IL‑4 DCs exhibited more mature phenotypes, and the levels of naïve allogeneic T cell stimulation were greater compared with GM‑CSF DCs. Phosphorylated signal transducer and activator of transcription 6 (p‑STAT6), the active form of STAT6, was expressed in GM‑CSF + IL‑4 DCs but not in GM‑CSF DCs. The present study demonstrated that IL‑4 influences DC morphology and immune function, and that this process may be associated with the activation of STAT6.
authors: Department of Oncology Institute, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Shizhong
authors: S
authors: Wang
authors: Department of Oncology Institute, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Xiao
authors: X
authors: Sun
authors: Department of Oncology Institute, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Haijun
authors: H
authors: Zhou
authors: Department of Oncology Institute, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Zhichao
authors: Z
authors: Zhu
authors: Department of Oncology Institute, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Wenjie
authors: W
authors: Zhao
authors: Department of General Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
authors: Chunfu
authors: C
authors: Zhu
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010641:Phenotype; D061251:Primary Cell Culture; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D050800:STAT6 Transcription Factor / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497966:Cd86 protein, rat; D050800:STAT6 Transcription Factor; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor