title: Effects of nano-TiO2 on antibiotic resistance transfer mediated by RP4 plasmid.
abstract: The potential risks of nano-materials and the spread of antibiotic resistance genes (ARGs) have become two major global public concerns. Studies have confirmed that nano-alumina can promote the spread of ARGs mediated by plasmids. Nano-titanium dioxide (TiO(2)), an excellent photocatalytic nano-material, has been widely used and is often present in aqueous environments. At various nano-material concentrations, bacterial density, matting time, and matting temperature, nano-TiO(2) can significantly promote the conjugation of RP4 plasmid in Escherichia coli. We developed a mathematical model to quantitatively describe the conjugation process and used this model to evaluate the effects of nano-TiO(2) on the spread of ARGs. We obtained analytical solutions for total and resistant bacteria, which were enumerated by the abundance of genetic loci unique to the plasmid and the chromosome using qPCR. Our results showed that the mathematic model was able to fit the experimental data well and can be used to quantitatively evaluate the effects of nano-TiO(2). According to our model, the presence of nano-TiO(2) decreased the bacterial growth rate from 0.0360 to 0.0323 min(-1) and increased the conjugative transfer rate from 6.69 × 10(-12) to 3.93 × 10(-10 )mL cell(-1) min(-1). These results indicate that nano-TiO(2) inhibited bacterial growth and promoted conjugation simultaneously. The data for morphology and mRNA expression also demonstrated this phenomenon. Our results confirm that environmental nano-TiO(2) may cause the spread of ARGs and thus poses an environmental risk. In addition, we provide a potential method for monitoring changes in ARGs that result from conjugation and evaluating the effects of antimicrobial substances on ARG expression.
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Zhigang
authors: Z
authors: Qiu
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Zhiqiang
authors: Z
authors: Shen
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Di
authors: D
authors: Qian
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Min
authors: M
authors: Jin
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Dong
authors: D
authors: Yang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Jingfeng
authors: J
authors: Wang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Bin
authors: B
authors: Zhang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Zhongwei
authors: Z
authors: Yang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Zhaoli
authors: Z
authors: Chen
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Xinwei
authors: X
authors: Wang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Chengshi
authors: C
authors: Ding
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Daning
authors: D
authors: Wang
authors: a Institute of Health and Environmental Medicine , Tianjin , China and.|b Key Laboratory of Risk Assessment and Control for Environment & Food Safety , Tianjin , China.
authors: Jun-Wen
authors: JW
authors: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000187:drug effects* / Q000235:genetics*; D054750:Ecotoxicology / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D049329:Nanostructures / Q000633:toxicity*; D010957:Plasmids / Q000187:drug effects* / Q000235:genetics*; D014025:Titanium / Q000633:toxicity*; D014873:Water Pollutants / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D014873:Water Pollutants; C009495:titanium dioxide; D014025:Titanium
keywords: Conjugation; conjugative transfer rate; environmental risk; growth rate; public concerns; spread of ARGs