title: Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner.
abstract: Neural stem and progenitor cell (NSPC) fate is strongly influenced by mechanotransduction as modulation of substrate stiffness affects lineage choice. Other types of mechanical stimuli, such as stretch (tensile strain), occur during CNS development and trauma, but their consequences for NSPC differentiation have not been reported. We delivered a 10% static equibiaxial stretch to NSPCs and examined effects on differentiation. We found static stretch specifically impacts NSPC differentiation into oligodendrocytes, but not neurons or astrocytes, and this effect is dependent on particular extracellular matrix (ECM)-integrin linkages. Generation of oligodendrocytes from NSPCs was reduced on laminin, an outcome likely mediated by the α6 laminin-binding integrin, whereas similar effects were not observed for NSPCs on fibronectin. Our data demonstrate a direct role for tensile strain in dictating the lineage choice of NSPCs and indicate the dependence of this phenomenon on specific substrate materials, which should be taken into account for the design of biomaterials for NSPC transplantation.
authors: 1] Department of Biomedical Engineering, University of California, Irvine, 3210 Natural Sciences II, Irvine, CA 92697-2715, USA [2] Sue &Bill Gross Stem Cell Research Center, University of California, Irvine, 845 Health Sciences Road, 3030 Gross Hall, Irvine, CA 92697-1705, USA.
authors: Janahan
authors: J
authors: Arulmoli
authors: Department of Physiology &Biophysics, University of California, Irvine, D380 Medical Sciences I, Irvine, CA 92697-4560, USA.
authors: Medha M
authors: MM
authors: Pathak
authors: 1] Sue &Bill Gross Stem Cell Research Center, University of California, Irvine, 845 Health Sciences Road, 3030 Gross Hall, Irvine, CA 92697-1705, USA [2] Department of Neurology, University of California, Irvine, 200 South Manchester Avenue Suite 206, Orange, CA 92868-4280, USA.
authors: Lisa P
authors: LP
authors: McDonnell
authors: 1] Sue &Bill Gross Stem Cell Research Center, University of California, Irvine, 845 Health Sciences Road, 3030 Gross Hall, Irvine, CA 92697-1705, USA [2] Department of Neurology, University of California, Irvine, 200 South Manchester Avenue Suite 206, Orange, CA 92868-4280, USA.
authors: Jamison L
authors: JL
authors: Nourse
authors: Department of Physiology &Biophysics, University of California, Irvine, D380 Medical Sciences I, Irvine, CA 92697-4560, USA.
authors: Francesco
authors: F
authors: Tombola
authors: 1] Department of Biomedical Engineering, University of California, Irvine, 3210 Natural Sciences II, Irvine, CA 92697-2715, USA [2] Department of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, CA 92697-2575, USA.
authors: James C
authors: JC
authors: Earthman
authors: 1] Department of Biomedical Engineering, University of California, Irvine, 3210 Natural Sciences II, Irvine, CA 92697-2715, USA [2] Sue &Bill Gross Stem Cell Research Center, University of California, Irvine, 845 Health Sciences Road, 3030 Gross Hall, Irvine, CA 92697-1705, USA [3] Department of Neurology, University of California, Irvine, 200 South Manchester Avenue Suite 206, Orange, CA 92868-4280, USA.
authors: Lisa A
authors: LA
authors: Flanagan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology; D011485:Protein Binding; D013314:Stress, Mechanical
chemical_list: D016023:Integrins; D007797:Laminin