title: Deacetyl-mycoepoxydiene, isolated from plant endophytic fungi Phomosis sp. demonstrates anti-microtubule activity in MCF-7 cells.
abstract: Deacetyl-mycoepoxydiene (DM), a novel secondary metabolite produced by the plant endophytic fungi Phomosis sp., induced the reorganization of cytoskeleton in actively growing MCF-7 cells by promoting polymerization of tubulin. DM could induce cell cycle arrest at G2/M in MCF-7 cells. Additionally, DM-induced apoptosis was characterized with up-regulating caspase-3, Bax, caspase-9, parp, and p21 while down-regulating Bcl-2 activation. DM conferred dose- and time-dependent inhibitory effects upon cell proliferation of MCF-7 cells both in cultured cells and nude mice with human breast carcinoma xenografts. The results obtained from these in vitro and in vivo models provide new data revealing the potential for DM as a novel microtubule inhibitor.
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors: Shan-Shan
authors: SS
authors: Zhu
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors: Yu-Sheng
authors: YS
authors: Zhang
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors: Xie-Huang
authors: XH
authors: Sheng
authors: University of Toronto, Toronto, Canada.
authors: Miao
authors: M
authors: Xu
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China; Department of Pharmacy, 456 Hospital of the People's Liberation Army, Jinan 250012, China.
authors: Si-Si
authors: SS
authors: Wu
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors: Yue-Mao
authors: YM
authors: Shen
authors: College of Life Sciences, Xiamen University, Xiamen 361005, China.
authors: Yao-Jian
authors: YJ
authors: Huang
authors: Technology Center, Xiwang Group Limited Company, Zouping 256209, China.
authors: Yi
authors: Y
authors: Wang
authors: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: shiyq@sdu.edu.cn.
authors: Yan-Qiu
authors: YQ
authors: Shi
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D060026:Endophytes / Q000737:chemistry*; D005260:Female; D005658:Fungi / Q000737:chemistry*; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D058105:Polymerization; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D001952:Bridged-Ring Compounds; D011753:Pyrones; C000602102:deacetyl-mycoepoxydiene; C476085:mycoepoxydiene
keywords: Apoptosis; Cell cycle arrest; Microtubule inhibitor