title: Ameliorative Effects of Testosterone Administration on Renal Redox Homeostasis in Naturally Aged Rats.
abstract: BACKGROUND
  Testosterone biosynthesis gradually decreases with age. Impaired redox homeostasis-related oxidative damage in cellular macromolecules has a high risk for the development of renal insufficiency. Our aim was to study the effects of testosterone replacement therapy on redox homeostasis.
  
  
  METHODS
  We investigated various oxidative damage biomarkers in kidney. Experimental animals were separated into three groups-naturally aged rats, testosterone-administered naturally aged rats (single dose of 25 mg/kg testosterone enanthate), and their respective young controls.
  
  
  RESULTS
  Our results showed that the testosterone-administered naturally aged group shared significant similarities with the young rats with respect to their redox status. In testosterone-administered naturally aged rats, kynurenine, protein carbonyl, advanced oxidation protein products, lipid peroxidation markers, and xanthine oxidase activities were significantly lower and Cu-Zn superoxide dismutase activities and testosterone levels were higher than naturally aged rats. In testosterone-administered naturally aged rats, catalase activities, ferric reducing anti-oxidant power, and testosterone levels were significantly lower and dityrosine, N-formyl kynurenine, protein carbonyl, and protein hydroperoxides were significantly higher than in young rats. On the other hand, in naturally aged rats, Cu-Zn superoxide dismutase, catalase activities, ferric reducing anti-oxidant power, and testosterone levels were lower and dityrosine, kynurenine, protein carbonyl, protein hydroperoxide, advanced oxidation protein products, lipid peroxidation markers, advanced glycation end products, and xanthine oxidase activities were higher than controls.
  
  
  CONCLUSIONS
  Our results showed that a single dose of testosterone administration has a positive effect on the redox status of the aged kidney. Future studies are needed to clarify the exact molecular mechanism(s) involved in the action of testosterone in maintaining kidney redox homeostasis.
authors: 1 Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Karolin
authors: K
authors: Yanar
authors: 1 Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Pınar
authors: P
authors: Atukeren
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Tamer
authors: T
authors: Cebe
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Ahmad
authors: A
authors: Kunbaz
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Tuna
authors: T
authors: Ozan
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Ahmet Doğukan
authors: AD
authors: Kansu
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Selahattin
authors: S
authors: Durmaz
authors: 2 Basic Sciences, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Veysel
authors: V
authors: Güleç
authors: 3 Department of Nursing, Faculty of Health Sciences, Bezmialem Vakıf University , Istanbul, Turkey .
authors: Ahmet
authors: A
authors: Belce
authors: 1 Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Seval
authors: S
authors: Aydın
authors: 1 Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors: Ufuk
authors: U
authors: Çakatay
authors: 4 Department of Biochemistry, University of Allahabad , Allahabad, India .
authors: Syed Ibrahim
authors: SI
authors: Rizvi
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D024322:Amino Acids, Aromatic / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects*; D007267:Injections; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D024322:Amino Acids, Aromatic; D015415:Biomarkers; D011506:Proteins; D013739:Testosterone; D003404:Creatinine