title: Characterization of influenza virus among influenza like illness cases in Mumbai, India.
abstract: The present study was carried out to monitor influenza viruses by identifying the virus and studying the seasonal variation during 2007-2009 in Mumbai. A total of 193 clinical respiratory samples (nasal and throat swab) were collected from patients having influenza like illness in Mumbai region. One-step real-time reverse-transcriptase PCR (rRTPCR) was used to detect Influenza type A (H1 and H3) and Influenza type B virus. Isolation of the virus was carried out using in vitro system which was further confirmed and typed by hemagglutination assay and hemagglutination inhibition assay. Out of 193 samples 24 (12.4 3%) samples tested positive for influenza virus, of which 13 (6.73 %) were influenza type A virus and 10 (5.18 %) were influenza type B virus, while 1 sample (0.51 %) was positive for both. By culture methods, 3 (1.55 %) viral isolates were obtained. All the three isolates were found to be Influenza type B/Malaysia (Victoria lineage) by Hemagglutination Inhibition Assay. The data generated from the present study reveals that both Influenza type A and B are prevalent in Mumbai with considerable activity. The peak activity was observed during monsoon season.
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Soumen
authors: S
authors: Roy
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Ritwik
authors: R
authors: Dahake
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Deepak
authors: D
authors: Patil
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Shweta
authors: S
authors: Tawde
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Sandeepan
authors: S
authors: Mukherjee
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Shrikant
authors: S
authors: Athlekar
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Abhay
authors: A
authors: Chowdhary
authors: Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India.
authors: Ranjana
authors: R
authors: Deshmukh
keywords: ILI cases; Influenza virus; Phylogeny; rRT PCR