title: [MicroRNA-10a expression in FAB different subtype of acute myeloid leukemia and its relationship with drug resistance].
abstract: OBJECTIVE
  This study was to investigate the expression of miR-10a in the different FAB subtype of acute myeloid leukemia (AML) and its relationship with drug resistance.
  
  
  METHODS
  Forty de novo patients with AML, 16 patients with non-malignant hematologic disease and three AML cell lines HL-60, U937 and HL-60/ADR were enrolled in this study, the MiR-10a expression in bone marrow mononuclear cells of above-mentioned patients and 3 AML cell lines was detected by TaqMan RT-PCR. The correlation of miR-10a with clinicopathological factors of AML patients was analyzed.
  
  
  RESULTS
  The miR-10a expression level in HL-60 cell line was higher than that in U937 cell line (P = 0.039). And its expression level in de novo AML patients was higher than that in patients with non-malignant hematologic disease (P < 0.01). FAB-AML-M3 patients exhibited higher expression of miR-10a than that in M1, M2 and M4 (P < 0.05); HL-60/ADR cell line showed higher miR-10a expression than that in HL-60 cell line (P < 0.01) . Except M3, the patients without CR (non-CR) after the first cycle of chemotherapy showed a higher level of miR-10a as compared with CR patients (P < 0.01).
  
  
  CONCLUSIONS
  The high expression of miR-10a may be closely related to over-proliferation of promyelocyte and drug resistance of acute myeloid leukemia cells, except M3.
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Yong-Jin
authors: YJ
authors: Zhi
authors: Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Feng
authors: F
authors: Zhi
authors: Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Rong
authors: R
authors: Wang
authors: Research Center of Modern Medicine, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Lian
authors: L
authors: Xue
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Wei-Ying
authors: WY
authors: Gu
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Biao
authors: B
authors: Wang
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Wei-Min
authors: WM
authors: Dong
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Hai-Qian
authors: HQ
authors: Li
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Yun
authors: Y
authors: Ling
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Guo-Qiang
authors: GQ
authors: Qiu
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Zhi-Lin
authors: ZL
authors: Wang
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China.
authors: Xiang-Shan
authors: XS
authors: Cao
authors: Department of Hematology, The Third Hospital Affiliated to Soochow University, Changzhou 213003, Jiangsu Province, China. E-mail:15051973544@163.com.
authors: Yan
authors: Y
authors: Liu
mesh_terms: D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D035683:MicroRNAs
chemical_list: C547183:MIRN10 microRNA, human; D035683:MicroRNAs