title: Reversible energy-transfer switching on a DNA scaffold.
abstract: We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.
authors: Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology , 412 96 Göteborg, Sweden.
authors: Magnus
authors: M
authors: Bälter
authors: Martin
authors: M
authors: Hammarson
authors: Patricia
authors: P
authors: Remón
authors: Shiming
authors: S
authors: Li
authors: Nittaya
authors: N
authors: Gale
authors: Tom
authors: T
authors: Brown
authors: Joakim
authors: J
authors: Andréasson
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D005456:Fluorescent Dyes; D004247:DNA