title: Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL).
abstract: A periplasmic sensory domain of the Campylobacter jejuni chemoreceptor for multiple ligands (CcmL) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. A complete data set was collected to 1.3 Å resolution using cryocooling conditions and synchrotron radiation. The crystals belonged to space group P21, with unit-cell parameters a = 42.6, b = 138.0, c = 49.0 Å, β = 94.3°.
authors: Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
authors: Mayra A
authors: MA
authors: Machuca
authors: Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
authors: Yu C
authors: YC
authors: Liu
authors: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
authors: Simone A
authors: SA
authors: Beckham
authors: Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
authors: Anna
authors: A
authors: Roujeinikova
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002167:Campylobacter / Q000737:chemistry*; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008027:Light; D008969:Molecular Sequence Data; D058849:Protein Refolding; D017434:Protein Structure, Tertiary; D012542:Scattering, Radiation
chemical_list: D001426:Bacterial Proteins; D008024:Ligands
keywords: Campylobacter jejuni; chemotaxis; methyl-accepting proteins; transducer-like proteins