title: In vivo application of chitosan to improve bioavailability of cyanocobalamin, a form of vitamin B12, following intraintestinal administration in rats.
abstract: The effect of chitosan on the intestinal absorption of cyanocobalamin (VB12), a stable form of vitamin B12, was investigated in vivo in rats, with the aim of improving the oral bioavailability of VB12 for anemia treatment in patients with gastrectomy. The bioavailability was evaluated based on the plasma concentration profile of VB12 following intraintestinal administration of the VB12 solution containing chitosan at various concentrations. The bioavailability of VB12 was 0.6±0.2% when the chitosan-free VB12 solution was administered, while it increased to 10.5±3.3% when chitosan was dissolved in the VB12 solution at a concentration of 1%. The bioavailability of VB12 increases with the chitosan concentration, in which chitosan seems to augment the amount of VB12 absorbed without affecting the absorption rate constant of VB12. It was also shown that the bioavailability of VB12 does not increase further when the degree of chitosan deacetylation is increased from 83 to 100% by substitutively employing the fully deacetylated chitosan. These findings suggest that the oral administration of VB12 with readily available chitosan may be a practical approach for anemia treatment in patients with gastrectomy.
authors: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
authors: Yuko
authors: Y
authors: Goto
authors: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
authors: Ayumi
authors: A
authors: Masuda
authors: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan. Electronic address: taiba@cc.okayama-u.ac.jp.
authors: Tetsuya
authors: T
authors: Aiba
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001682:Biological Availability; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D007408:Intestinal Absorption; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014805:Vitamin B 12 / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*
chemical_list: D048271:Chitosan; D014805:Vitamin B 12
keywords: Anemia; Bioavailability; Chitosan; Cyanocobalamin; Gastrectomy; Vitamin B(12)