title: Two nucleoside receptors from Streptomyces coelicolor: expression of the genes and characterization of the recombinant proteins.
abstract: Streptomyces coelicolor is a soil-dwelling bacterium that undergoes an intricate, saprophytic lifecycle. The bacterium takes up exogenous nucleosides for nucleic acid synthesis or use as carbon and energy sources. However, nucleosides must pass through the membrane with the help of transporters. In the present work, the SCO4884 and SCO4885 genes were cloned into pCOLADuet-1 and overexpressed in Escherichia coli BL21. Each protein was monomeric. Using isothermal titration calorimetry, we determined that SCO4884 and SCO4885 are likely nucleoside receptors with affinity for adenosine and pyrimidine nucleosides. On the basis of bioinformatics analysis and the transporter classification system, we speculate that SCO4884-SCO4888 is an ABC-like transporter responsible for the uptake of adenosine and pyrimidine nucleosides.
authors: State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China; School of Marine Science and Technology, Jiangsu Marine Resources Development Research Institute, Huaihai Institute of Technology, Lianyungang, Jiangsu Province 222005, People's Republic of China.
authors: Fuhou
authors: F
authors: Li
authors: State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China.
authors: Jingdan
authors: J
authors: Liang
authors: School of Marine Science and Technology, Jiangsu Marine Resources Development Research Institute, Huaihai Institute of Technology, Lianyungang, Jiangsu Province 222005, People's Republic of China.
authors: Weixia
authors: W
authors: Wang
authors: State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China.
authors: Xiufen
authors: X
authors: Zhou
authors: State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China. Electronic address: zxdeng@sjtu.edu.cn.
authors: Zixin
authors: Z
authors: Deng
authors: State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China. Electronic address: wangzhijun@sjtu.edu.cn.
authors: Zhijun
authors: Z
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009705:Nucleosides / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*; D016415:Sequence Alignment; D048372:Streptomyces coelicolor / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D009705:Nucleosides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins
keywords: Nucleoside receptor; SCO4884; SCO4885; Streptomyces coelicolor