title: Hematopoietic activity in putative mouse primordial germ cell populations.
abstract: In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.
authors: Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.
authors: Maria Lucia
authors: ML
authors: Scaldaferri
authors: Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.
authors: Francesca Gioia
authors: FG
authors: Klinger
authors: Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.
authors: Donatella
authors: D
authors: Farini
authors: Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.
authors: Anna
authors: A
authors: Di Carlo
authors: Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Laboratory of Flow-Cytometry and B Cell Development, Rome, Italy.
authors: Rita
authors: R
authors: Carsetti
authors: Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Laboratory of Flow-Cytometry and B Cell Development, Rome, Italy.
authors: Ezio
authors: E
authors: Giorda
authors: Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy. Electronic address: defelici@uniroma2.it.
authors: Massimo
authors: M
authors: De Felici
mesh_terms: D000818:Animals; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D006066:Gonads / Q000166:cytology / Q000378:metabolism*; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D008650:Mesonephros / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism
chemical_list: D050814:Octamer Transcription Factor-3
keywords: Allantois; Germline; Hematopoiesis; Oct3/4; Primordial germ cells