title: Telomerase expression in individuals with chronic and aggressive periodontitis.
abstract: BACKGROUND
  The aim of this study is to evaluate the expression of human telomerase reverse transcription (hTERT) enzyme in chronic periodontitis (CP) and aggressive periodontitis (AgP) compared with healthy individuals.
  
  
  METHODS
  A total of 79 individuals consented to participate in the study. The study sample comprised healthy individuals (n = 30), patients with CP (n = 30), and patients with AgP (n = 19). Gingival tissue was collected and evaluated for hTERT expression by Western blot and immunohistochemical methods. Reverse transcription polymerase chain reaction was performed using the gingival crevicular fluid (GCF) samples.
  
  
  RESULTS
  The hTERT messenger RNA (mRNA) and protein expression was significantly higher in AgP compared with CP (P <0.001). In GCF, 53.33% of patients with CP and 68.42% of patients with AgP were showing hTERT mRNA expression, but it was not detected in the control group. The AgP tissue showed higher hTERT expression compared with CP (P <0.001). The hTERT mRNA expression did not show a correlation with gingival index (GI), plaque index (PI), probing depth (PD), and clinical attachment loss (AL) in patients with AgP, whereas hTERT protein expression was strongly correlated with GI, PI, PD, and AL in patients with AgP. The protein expression of hTERT shows significant but moderate correlation with GI and AL in patients with CP.
  
  
  CONCLUSIONS
  High expression of hTERT might be associated with periodontal disease progression, suggesting that hTERT could be a potential prognostic marker.
authors: Molecular and Human Genetics Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology, Kolkata, India.
authors: Atul
authors: A
authors: Katarkar
authors: Arindam
authors: A
authors: Saha
authors: Sanjit
authors: S
authors: Mukherjee
authors: Debabrata
authors: D
authors: Kundu
authors: Prasanta
authors: P
authors: Bandyopadhyay
authors: Keya
authors: K
authors: Chaudhuri
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000201:enzymology*; D015415:Biomarkers / Q000032:analysis; D055113:Chronic Periodontitis / Q000201:enzymology*; D003238:Connective Tissue / Q000201:enzymology; D003774:Dental Plaque Index; D004848:Epithelium / Q000201:enzymology; D005260:Female; D005881:Gingiva / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000201:enzymology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000032:analysis*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D019098:Telomerase
keywords: Aggressive periodontitis; chronic periodontitis; gingival crevicular fluid; gingival recession; periodontal attachment loss; telomerase.