title: Crystallization and preliminary X-ray diffraction studies of the family 54 carbohydrate-binding module from laminarinase (β-1,3-glucanase) Lic16A of Clostridium thermocellum.
abstract: The crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding module (CBM) from laminarinase Lic16A of the hyperthermophilic anaerobic bacterium Clostridium thermocellum (ctCBM54) are reported. Recombinant ctCBM54 was prepared using an Escherichia coli/pQE30 overexpression system and was crystallized by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 Å resolution using synchrotron radiation. The crystals belonged to space group P6322, with unit-cell parameters a = b = 130.15, c = 131.05 Å. The three-dimensional structure of ctCBM54 will provide valuable information about the structure-function relation of the laminarinase Lic16A and will allow the exploitation of this binding module in biotechnological applications.
authors: A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninsky Prospect 59, Moscow 117333, Russian Federation.
authors: Yury A
authors: YA
authors: Kislitsyn
authors: A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninsky Prospect 59, Moscow 117333, Russian Federation.
authors: Valeriya R
authors: VR
authors: Samygina
authors: Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russian Federation.
authors: Igor A
authors: IA
authors: Dvortsov
authors: Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russian Federation.
authors: Nataliya A
authors: NA
authors: Lunina
authors: A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninsky Prospect 59, Moscow 117333, Russian Federation.
authors: Inna P
authors: IP
authors: Kuranova
authors: Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russian Federation.
authors: Galina A
authors: GA
authors: Velikodvorskaya
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D044602:Cellulases / Q000737:chemistry*; D048013:Acetivibrio thermocellus / Q000201:enzymology*; D003460:Crystallization; D004058:Diffusion; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D044602:Cellulases
keywords: Clostridium thermocellum; Lic16A; carbohydrate-binding module; β-1,3-glucanase