title: The molecular architecture of dihydropyrindine receptor/L-type Ca2+ channel complex.
abstract: Dihydropyridine receptor (DHPR), an L-type Ca(2+) channel complex, plays an essential role in muscle contraction, secretion, integration of synaptic input in neurons and synaptic transmission. The molecular architecture of DHPR complex remains elusive. Here we present a 15-Å resolution cryo-electron microscopy structure of the skeletal DHPR/L-type Ca(2+) channel complex. The DHPR has an asymmetrical main body joined by a hook-like extension. The main body is composed of a "trapezoid" and a "tetrahedroid". Homologous crystal structure docking and site-specific antibody labelling revealed that the α1 and α2 subunits are located in the "trapezoid" and the β subunit is located in the "tetrahedroid". This structure revealed the molecular architecture of a eukaryotic Ca(2+) channel complex. Furthermore, this structure provides structural insights into the key elements of DHPR involved in physical coupling with the RyR/Ca(2+) release channel and shed light onto the mechanism of excitation-contraction coupling.
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Hongli
authors: H
authors: Hu
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Zhao
authors: Z
authors: Wang
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Risheng
authors: R
authors: Wei
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Guizhen
authors: G
authors: Fan
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Qiongling
authors: Q
authors: Wang
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Kaiming
authors: K
authors: Zhang
authors: Department of Biophysics, Peking University Health Science Centre, Peking University, 38 College Road, Beijing 100191, China.
authors: Chang-Cheng
authors: CC
authors: Yin
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020746:Calcium Channels, L-Type; D009124:Muscle Proteins; D021122:Protein Subunits; D019837:Ryanodine Receptor Calcium Release Channel