title: Sensory inputs control the integration of neurogliaform interneurons into cortical circuits.
abstract: Neuronal microcircuits in the superficial layers of the mammalian cortex provide the substrate for associative cortical computation. Inhibitory interneurons constitute an essential component of the circuitry and are fundamental to the integration of local and long-range information. Here we report that, during early development, superficially positioned Reelin-expressing neurogliaform interneurons in the mouse somatosensory cortex receive afferent innervation from both cortical and thalamic excitatory sources. Attenuation of ascending sensory, but not intracortical, excitation leads to axo-dendritic morphological defects in these interneurons. Moreover, abrogation of the NMDA receptors through which the thalamic inputs signal results in a similar phenotype, as well as in the selective loss of thalamic and a concomitant increase in intracortical connectivity. These results suggest that thalamic inputs are critical in determining the balance between local and long-range connectivity and are fundamental to the proper integration of Reelin-expressing interneurons into nascent cortical circuits.
authors: 1] NYU Neuroscience Institute, New York Langone Medical Center, New York, New York, USA. [2] Department of Neuroscience and Physiology, New York Langone Medical Center, New York, New York, USA. [3] Department of Neural Science, New York University, New York, New York, USA.
authors: Natalia V
authors: NV
authors: De Marco García
authors: 1] NYU Neuroscience Institute, New York Langone Medical Center, New York, New York, USA. [2] Department of Neuroscience and Physiology, New York Langone Medical Center, New York, New York, USA. [3] Department of Neural Science, New York University, New York, New York, USA.
authors: Rashi
authors: R
authors: Priya
authors: 1] NYU Neuroscience Institute, New York Langone Medical Center, New York, New York, USA. [2] Department of Neuroscience and Physiology, New York Langone Medical Center, New York, New York, USA. [3] Department of Neural Science, New York University, New York, New York, USA.
authors: Sebnem N
authors: SN
authors: Tuncdemir
authors: 1] NYU Neuroscience Institute, New York Langone Medical Center, New York, New York, USA. [2] Department of Neuroscience and Physiology, New York Langone Medical Center, New York, New York, USA. [3] Department of Neural Science, New York University, New York, New York, USA.
authors: Gord
authors: G
authors: Fishell
authors: 1] NYU Neuroscience Institute, New York Langone Medical Center, New York, New York, USA. [2] Department of Neuroscience and Physiology, New York Langone Medical Center, New York, New York, USA. [3] Department of Neural Science, New York University, New York, New York, USA.
authors: Theofanis
authors: T
authors: Karayannis
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002540:Cerebral Cortex / Q000166:cytology*; D018274:Electroporation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009415:Nerve Net / Q000502:physiology*; D011247:Pregnancy; D022661:RNA, Untranslated / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics; D000091042:Reelin Protein; D014157:Transcription Factors / Q000235:genetics; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics; D014738:Vibrissae / Q000294:innervation*; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D001640:Bicuculline; D018691:Excitatory Amino Acid Antagonists; D018398:Homeodomain Proteins; D022661:RNA, Untranslated; D016194:Receptors, N-Methyl-D-Aspartate; D044406:Receptors, Serotonin, 5-HT3; D000091042:Reelin Protein; D014157:Transcription Factors; D050599:Vesicular Glutamate Transport Protein 2; D000099260:Empty Spiracles Homeobox Proteins; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C493477:Slc17a6 protein, mouse; C000717128:Reln protein, mouse