title: Knockdown of the host cellular protein transportin 3 attenuates prototype foamy virus infection.
abstract: Transportin 3 (TNPO3) is a member of the importin-ß superfamily proteins. Despite numerous studies, the exact molecular mechanism of TNPO3 in retroviral infection is still controversial. Here, we provide evidence for the role and mechanism of TNPO3 in the replication of prototype foamy virus (PFV). Our findings revealed that PFV infection was reduced 2-fold by knockdown (KD) of TNPO3. However, late stage of viral replication including transcription, translation, viral assembly, and release was not influenced. The differential cellular localization of PFV integrase (IN) in KD cells pinpointed a remarkable reduction of viral replication at the nuclear import step. We also found that TNPO3 interacted with PFV IN but not with Gag, suggesting that IN-TNPO3 interaction is important for nuclear import of PFV pre-integration complex. Our report enlightens the mechanism of PFV interaction with TNPO3 and support ongoing research on PFV as a promising safe vector for gene therapy.
authors: a Department of Systems Biotechnology , Chung Ang University , Ansung , Republic of Korea.
authors: Md Khadem
authors: MK
authors: Ali
authors: Jinsun
authors: J
authors: Kim
authors: Faysal Bin
authors: FB
authors: Hamid
authors: Cha-Gyun
authors: CG
authors: Shin
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D055785:Gene Knockdown Techniques; D019426:Integrases / Q000378:metabolism; D016092:Spumavirus / Q000201:enzymology / Q000502:physiology*; D028961:beta Karyopherins / Q000172:deficiency* / Q000235:genetics*
chemical_list: D028961:beta Karyopherins; D019426:Integrases
keywords: integrase; pre-integration complex; prototype foamy virus; transportin 3