title: Effect of pretreatment on arabinoxylan distribution in wheat bran.
abstract: Arabinoxylan is one of the potential key products of a wheat bran based biorefinery. To develop a suitable process for the isolation of arabinoxylans, the effect of different processing approaches needs to be determined. In this work, chemical analysis was supplemented by immunolocalization of arabinoxylan by confocal microscopy, which proved valuable in the assessment of cell-structural changes occurring upon different chemical and mechanical bran treatments. The influences of acid, lye and hydrogen peroxide treatment, ball-milling, extrusion, fermentation and treatment with esterase, xylanase and a combination thereof were investigated. Extensive ball-milling showed the best selectivity for harvesting arabinoxylan. Chemical treatments gave the highest yields, but did so at the cost of selectivity. Fermentative and enzymatic treatments were hampered by coextraction of other compounds.
authors: Department of Chemistry, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria; Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria.
authors: Johannes
authors: J
authors: Hell
authors: Scion, 49 Sala Street, Private Bag 3020, Rotorua 3046, New Zealand.
authors: Lloyd
authors: L
authors: Donaldson
authors: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria.
authors: Herbert
authors: H
authors: Michlmayr
authors: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria.
authors: Manuel
authors: M
authors: Kraler
authors: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria.
authors: Wolfgang
authors: W
authors: Kneifel
authors: Department of Chemistry, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria.
authors: Antje
authors: A
authors: Potthast
authors: Department of Chemistry, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria.
authors: Thomas
authors: T
authors: Rosenau
authors: Department of Chemistry, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria. Electronic address: stefan.boehmdorfer@boku.ac.at.
authors: Stefan
authors: S
authors: Böhmdorfer
mesh_terms: D004043:Dietary Fiber / Q000032:analysis* / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005285:Fermentation; D005511:Food Handling; D006861:Hydrogen Peroxide / Q000494:pharmacology; D055595:Mechanical Phenomena; D000432:Methanol / Q000737:chemistry; D014990:Xylans / Q000032:analysis*
chemical_list: D004043:Dietary Fiber; D014990:Xylans; C085118:arabinoxylan; D006861:Hydrogen Peroxide; D043364:Endo-1,4-beta Xylanases; D000432:Methanol
keywords: Arabinoxylan; Confocal microscopy; Enzymatic peeling; Immunolocalization; Methanolysis; Wheat bran