title: Development of an immunochromatographic strip for rapid detection of Pantoea stewartii subsp. stewartii.
abstract: A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 10(5) cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.
authors: Huaian Entry-Exit Inspection and Quarantine Bureau, Huaian 223001, China. fm_8228@163.com.|State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. fm_8228@163.com.
authors: Min
authors: M
authors: Feng
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. kdz19900910@163.com.
authors: Dezhao
authors: D
authors: Kong
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wenbin66@yeah.net.
authors: Wenbing
authors: W
authors: Wang
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. raxray@gmail.com.
authors: Liqiang
authors: L
authors: Liu
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. songshanshan0626@126.com.
authors: Shanshan
authors: S
authors: Song
authors: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. xcl@jiangnan.edu.cn.
authors: Chuanlai
authors: C
authors: Xu
mesh_terms: D000818:Animals; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D020636:Pantoea / Q000276:immunology* / Q000302:isolation & purification; D003313:Zea mays / Q000382:microbiology