title: Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance.
abstract: Incorporation of a piperazino-modified 2'-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3'-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.
authors: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. jwe@sdu.dk.
authors: Chenguang
authors: C
authors: Lou
authors: Birte
authors: B
authors: Vester
authors: Jesper
authors: J
authors: Wengel
mesh_terms: D003851:Deoxyribonucleases / Q000378:metabolism*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D000077489:Piperazine; D010879:Piperazines / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D010879:Piperazines; C527207:amino-LNA; D000077489:Piperazine; D003851:Deoxyribonucleases