title: Silver release and antimicrobial properties of PMMA films doped with silver ions, nano-particles and complexes.
abstract: Materials prepared on the base of bioactive silver compounds have become more and more popular due to low microbial resistance to silver. In the present work, the efficiency of polymethylmethacrylate (PMMA) thin films doped with silver ions, nanoparticles and silver-imidazole polymer complex was studied by a combination of AAS, XPS and AFM techniques. The biological activities of the proposed materials were discussed in view of the rate of silver releasing from the polymer matrix. Concentrations of Ag active form were estimated by its ability to interact with l-cysteine using electronic circular dichroism spectroscopy. Rates of the released silver were compared with the biological activity in dependence on the form of embedded silver. Antimicrobial properties of doped polymer films were studied using two bacterial strains: Staphylococcus epidermidis and Escherichia coli. It was found that PMMA films doped with Ag(+) had greater activity than those doped with nanoparticles and silver-imidazole polymeric complexes. However, the antimicrobial efficiency of Ag(+) doped films was only short-term. Contrary, the antimicrobial activity of silver-imidazole/PMMA films increased in time of sample soaking.
authors: Department of Solid State Engineering, Institute of Chemical Technology, Prague, Czech Republic. Electronic address: lyutakoo@vscht.cz.
authors: O
authors: O
authors: Lyutakov
authors: Department of Analytical Chemistry, Institute of Chemical Technology, Prague, Czech Republic.
authors: I
authors: I
authors: Goncharova
authors: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic.
authors: S
authors: S
authors: Rimpelova
authors: Department of Solid State Engineering, Institute of Chemical Technology, Prague, Czech Republic.
authors: K
authors: K
authors: Kolarova
authors: Department of Solid State Engineering, Institute of Chemical Technology, Prague, Czech Republic.
authors: J
authors: J
authors: Svanda
authors: Department of Solid State Engineering, Institute of Chemical Technology, Prague, Czech Republic.
authors: V
authors: V
authors: Svorcik
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D003545:Cysteine / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D007477:Ions / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry* / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D018030:Silver Compounds / Q000737:chemistry / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D007093:Imidazoles; D007477:Ions; D011108:Polymers; D018030:Silver Compounds; D012834:Silver; C029899:imidazole; D019904:Polymethyl Methacrylate; D003545:Cysteine
keywords: Antimicrobial properties; Doped polymer; Silver helical complex; Silver nanoparticles