title: Association of human papillomavirus type 58 with breast cancer in Shaanxi province of China.
abstract: A number of reports have identified HPV DNA in breast cancer specimens and HPV type 16, 18, 31, 33, 45, and 51 were more prevalent. HPV 58 was frequently detected in cervical cancer in Shaanxi China. The aim of the present study was to investigate whether HPV 58 present in breast cancer. 169 cases of breast cancer samples and 83 benign breast lesions were analyzed. Type specific primers and oligonucliotide probe were used for the detection of HPV 58 by conventional PCR and in situ hybridization techniques. The HPV 58 viral load were measured by qPCR. p16 protein expression were evaluated by immunohistochemistry. HPV 58 E7 DNA was detected in 25 out of 169 formalin fixed paraffin embedded breast cancer tissues (14.79%) by PCR, only 1 out of 83 non-malignant breast lesions showed positive (1.20%). The results of ISH showed that 17 out of 169 (10.06%) malignant samples were positive for HPV 58 E7, and only 1 out of 83 non-malignant lesions was positive. Positive p16 immunostaining was observed in all the HPV 58 E7 ISH positive cases, but 16 out of 98 cases with HPV negative were p16 positive. The presence of HPV 58 in both normal duct epithelial cells and carcinoma in situ along with its presence in the cancer cells of the same specimen indicated the possible causal role of HPV 58 in breast cancer. The findings provide a solid morphological evidence of the involvement of HPV 58 in breast cancer development.
authors: Center of Cancer Research, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China; Institute for Cancer Research, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors: Ling
authors: L
authors: Fu
authors: Depu
authors: D
authors: Wang
authors: Walayat
authors: W
authors: Shah
authors: Yili
authors: Y
authors: Wang
authors: Guanjun
authors: G
authors: Zhang
authors: Jianjun
authors: J
authors: He
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D002278:Carcinoma in Situ / Q000821:virology; D018270:Carcinoma, Ductal, Breast / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000821:virology; D002681:China; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology; D019562:Viral Load
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D017931:DNA Primers; D004279:DNA, Viral
keywords: HPV 58; ISH; PCR; breast cancer; high risk human papillomavirus