title: Transcription factors and anthocyanin genes related to low-temperature tolerance in rd29A:RdreB1BI transgenic strawberry.
abstract: Dehydration-responsive element-binding (DREB) transcription factors play critical roles in plant stress responses and signal transduction. To further understand how DREB regulates genes expression to promote cold-hardiness, Illumina/Solexa sequencing technology was used to compare the transcriptomes of non-transgenic and rd29A:RdreB1BI transgenic strawberry plants exposed to low temperatures. Approximately 3.5 million sequence tags were obtained from non-transgenic (NT) and transgenic (T) strawberry untreated (C) or low-temperature treated (LT) leaf samples. Over 1000 genes were differentially expressed between the NT-C and T-C plants, and also the NT-C and NT-LT, as well as the T-C and T-LT plants. Analysis of the genes up-regulated following low-temperature treatment revealed that the majority are linked to metabolism, biosynthesis, transcription and signal transduction. Uniquely up-regulated transcription factors as well as anthocyanin biosynthetic pathway genes are discussed. Accumulation of anthocyanin in the stolon and the base of the petiole differed between non-treated NT and T plants, and this correlated with gene expression patterns. The differentially expressed genes that encode transcription factors and anthocyanin enzymes may contribute to the cold hardiness of RdreB1BI transgenic strawberry. The transcriptome data provide a valuable resource for further studies of strawberry growth and development and DREB-mediated gene regulation under low-temperature stress.
authors: College of Horticulture, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China; College of Life Sciences, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
authors: Xianbin
authors: X
authors: Gu
authors: College of Life Sciences, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
authors: Yahua
authors: Y
authors: Chen
authors: College of Horticulture, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
authors: Zhihong
authors: Z
authors: Gao
authors: College of Horticulture, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
authors: Yushan
authors: Y
authors: Qiao
authors: College of Agro-grassland Science, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
authors: Xiuyun
authors: X
authors: Wang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000872:Anthocyanins / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D031985:Fragaria / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Digital gene expression; Low-temperature; RdreB1BI; Strawberry