title: Oregonin reduces lipid accumulation and proinflammatory responses in primary human macrophages.
abstract: Inflammation in the vascular wall is important for the development of atherosclerosis. We have previously shown that inflammatory macrophages are more abundant in human atherosclerotic lesions than in healthy arteries. Activated macrophages produce reactive oxygen species (ROS) that promote local inflammation in atherosclerotic lesions. Here, we investigated the role of oregonin, a diarylheptanoid, on proinflammatory responses in primary human macrophages and found that oregonin decreased cellular lipid accumulation and proinflammatory cytokine secretion. We also found that oregonin decreased ROS production in macrophages. Additionally, we observed that treatment of lipopolysaccharide-exposed macrophages with oregonin significantly induced the expression of antioxidant-related genes, including Heme oxygenase-1 and NADPH dehydrogenase quinone 1. In summary, we have shown that oregonin reduces lipid accumulation, inflammation and ROS production in primary human macrophages, indicating that oregonin has anti-inflammatory bioactivities.
authors: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, S-41345 Gothenburg, Sweden.
authors: Annika
authors: A
authors: Lundqvist
authors: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, S-41345 Gothenburg, Sweden.
authors: Lisa U
authors: LU
authors: Magnusson
authors: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, S-41345 Gothenburg, Sweden.
authors: Christina
authors: C
authors: Ullström
authors: Riga Stradin University, Riga, Latvia.
authors: Jelena
authors: J
authors: Krasilnikova
authors: Latvian State Institute of Wood Chemistry, Riga, Latvia.
authors: Galina
authors: G
authors: Telysheva
authors: Latvian State Institute of Wood Chemistry, Riga, Latvia.
authors: Tatjana
authors: T
authors: Dizhbite
authors: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, S-41345 Gothenburg, Sweden; Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden. Electronic address: lillemor.mattsson@wlab.gu.se.
authors: Lillemor Mattsson
authors: LM
authors: Hultén
mesh_terms: D000328:Adult; D029661:Alnus / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D050197:Atherosclerosis / Q000188:drug therapy / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D036381:Diarylheptanoids / Q000737:chemistry / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D008055:Lipids / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D036381:Diarylheptanoids; D008055:Lipids; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; C409456:oregonin; D051547:Heme Oxygenase-1
keywords: Anti-inflammatory; Cytokines; Lipid accumulation; Macrophages; Oregonin