title: Ectopic expression of AP-2α transcription factor suppresses glioma progression.
abstract: The transcriptional factor AP-2α is a tumor suppressor gene and is downregulated in various neoplasms including glioma. Although the level of AP-2α is negatively associated with the grade of human glioma, the specific functions of AP-2α in glioma are still unknown. In this study, we experimentally showed that artificial overexpression of AP-2α in glioma T98G and U251 cells significantly downregulated the mRNA levels of Bcl-xl, Bcl-2, c-IAP2 and survivin, together with upregulation of the Hrk mRNA levels. Reintroduction of AP-2α also induced downregulation of the protein levels of survivin and VEGF in glioma cells. In biological assays with T98G and U251 cells, AP-2α reduced tumor cell growth, increased cell death, attenuated cell migration and endothelial tube formation. The AP-2α transcription factor may play an important role in suppressing glioma progression.
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Wenjing
authors: W
authors: Su
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China ; Suining Central Hospital Suining 629000, China.
authors: Juan
authors: J
authors: Xia
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Xueqin
authors: X
authors: Chen
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Miao
authors: M
authors: Xu
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Ling
authors: L
authors: Nie
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Ni
authors: N
authors: Chen
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Jing
authors: J
authors: Gong
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Xinglan
authors: X
authors: Li
authors: Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
authors: Qiao
authors: Q
authors: Zhou
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D018450:Disease Progression; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D060888:Real-Time Polymerase Chain Reaction; D050656:Transcription Factor AP-2 / Q000096:biosynthesis*
chemical_list: D050656:Transcription Factor AP-2
keywords: AP-2 alpha; apoptosis; cell growth; glioma; invasion