title: Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.
abstract: Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.
authors: Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University, Tsu, Mie, Japan E-mail : tchihara@fujita-hu.ac.jp.
authors: Takeshi
authors: T
authors: Chihara
authors: Kan
authors: K
authors: Shimpo
authors: Takaaki
authors: T
authors: Kaneko
authors: Hidehiko
authors: H
authors: Beppu
authors: Takashi
authors: T
authors: Higashiguchi
authors: Shigeru
authors: S
authors: Sonoda
authors: Miyuki
authors: M
authors: Tanaka
authors: Muneo
authors: M
authors: Yamada
authors: Fumiaki
authors: F
authors: Abe
mesh_terms: D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000504:Aloe / Q000737:chemistry*; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000627:therapeutic use*; D011208:Powders / Q000737:chemistry / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D011208:Powders; D012333:RNA, Messenger; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase; D001397:Azoxymethane