title: Serum albumin coating of demineralized bone matrix results in stronger new bone formation.
abstract: Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.|Department of Orthopedics, Semmelweis University, Budapest, Hungary.
authors: Dénes B
authors: DB
authors: Horváthy
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.
authors: Gabriella
authors: G
authors: Vácz
authors: Department of Interfaces and Surface Modification, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors: Tamás
authors: T
authors: Szabó
authors: Department of Biological Nanochemistry, Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors: Imola C
authors: IC
authors: Szigyártó
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.
authors: Ildikó
authors: I
authors: Toró
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.
authors: Boglárka
authors: B
authors: Vámos
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.
authors: István
authors: I
authors: Hornyák
authors: Department of Physical Chemistry and Material Science, Budapest University of Technology and Economics, Budapest, Hungary.
authors: Károly
authors: K
authors: Renner
authors: Department of Orthopedics, Semmelweis University, Budapest, Hungary.
authors: Tamás
authors: T
authors: Klára
authors: Department of Oral Diagnostics, Semmelweis University, Budapest, Hungary.
authors: Bence T
authors: BT
authors: Szabó
authors: Department of Oral Diagnostics, Semmelweis University, Budapest, Hungary.
authors: Csaba
authors: C
authors: Dobó-Nagy
authors: Department of Transplantation and Surgery, Semmelweis University, Budapest, Hungary.
authors: Attila
authors: A
authors: Doros
authors: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary.|Department of Orthopedics, Semmelweis University, Budapest, Hungary.
authors: Zsombor
authors: Z
authors: Lacza
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin; D012886:Skull / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D012709:Serum Albumin
keywords: DBM; bone; calvaria defect; serum albumin; stem cells