title: A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
abstract: Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
authors: Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
authors: Lauren R
authors: LR
authors: Polstein
authors: 1] Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA. [2] Center for Genomic and Computational Biology, Duke University, Durham, North Carolina, USA. [3] Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA.
authors: Charles A
authors: CA
authors: Gersbach
mesh_terms: D064113:CRISPR-Cas Systems / Q000187:drug effects* / Q000528:radiation effects; D056931:Cryptochromes / Q000235:genetics; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics / Q000528:radiation effects; D006801:Humans; D008027:Light; D062308:Optogenetics; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: C538966:CRY2 protein, human; D056931:Cryptochromes; D012313:RNA; D004247:DNA