title: IL1RAP as a surface marker for leukemia stem cells is related to clinical phase of chronic myeloid leukemia patients.
abstract: Chronic myeloid leukemia (CML) is a clonal disease from hematopoietic stem cells. Surviving leukemia stem cells (LSCs) and progenitor cells are a potential source for CML relapse and progression. Recent data reported that IL-1 receptor accessory protein (IL1RAP) gene was differentially expressed in CML versus normal stem and progenitor cells. However, whether the level of IL1RAP is associated with clinical phases of CML, and correlations between IL1RAP expression and detections of diagnosis is still unclear. Here we demonstrated that IL1RAP was up-regulated in CD34+ and CD34+CD38- cells which highly enriched with stem cells. Furthermore, IL1RAP expression in CD34+CD38- cells was tightly consistent with the generation of BCR-ABL fusion gene and Philadelphia chromosome. Importantly, we found that the level of IL1RAP increased with disease progression from chronic phase (CP) into accelerated phase (AP) and blast phase (BP), which was investigated not only in new diagnosed CML patients but also in patients treated with tyrosine kinase inhibitors (TKI) and hydroxyurea. Negative correlation was detected between IL1RAP expression and neutrophil (NE), whereas no relation was found in white blood cell (WBC), lymphocyte (LY), red blood cell (RBC), platelet (PLT), age or gender of CML patients. In conclusion, we identified IL1RAP as a surface marker of LSCs may be a potential indicator for CML clinical phases.
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China ; Key Laboratory of Bone Marrow Stem Cell No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu Province, China ; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Kai
authors: K
authors: Zhao
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Ling-Ling
authors: LL
authors: Yin
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Dong-Mei
authors: DM
authors: Zhao
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China ; Key Laboratory of Bone Marrow Stem Cell No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu Province, China ; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Bin
authors: B
authors: Pan
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Wei
authors: W
authors: Chen
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Jiang
authors: J
authors: Cao
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Hai
authors: H
authors: Cheng
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Zhen-Yu
authors: ZY
authors: Li
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: De-Peng
authors: DP
authors: Li
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Wei
authors: W
authors: Sang
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China ; Key Laboratory of Bone Marrow Stem Cell No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu Province, China ; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Ling-Yu
authors: LY
authors: Zeng
authors: Blood Diseases Institute, Xuzhou Medical College No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China ; Key Laboratory of Bone Marrow Stem Cell No. 84 West Huaihai Road, Xuzhou 221002, Jiangsu Province, China ; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College No. 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China.
authors: Kai-Lin
authors: KL
authors: Xu
keywords: Chronic myeloid leukemia; IL-1 receptor accessory protein; leukemia stem cells