title: Effects of icariin on cytokine-induced ankylosing spondylitis with fibroblastic osteogenesis and its molecular mechanism.
abstract: The aim of this study is to explore the effects of icariin on cytokine induced ankylosing spondylitis fibroblast osteogenesis type expression and its molecular mechanism. The normal fibroblasts were collected as normal control group, and the fibroblasts of hip joint capsule of AS patients were collected, which were respectively added in fetal bovine serum (group AS), fetal bovine serum and cytokines (BMP-2+TGF-beta 1) (group AS), and cell factor solution (icariin group), and observed of the osteogenic expression of fibroblast, to evaluate the impact of Icariin on it. The ALP activity, the content of collagen, osteocalcin content and cbfa1mRNA and OCmRNA of fibroblast of AS group increased compared to the normal control group and AS control group (P < 0.01), indicating that icariin can significantly inhibit the above changes (P < 0.01). Icariin can inhibit fibroblast further osteogenic differentiation through inhibiting the effect of cytokines on the fibroblast osteogenesis type markers and osteogenic gene expression and osteogenic differentiation.
authors: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors: Chunrong
authors: C
authors: Jia
authors: Department of Rheumatology, Chinese Academy of Traditional Chinese Medicine, Guanganmen Hospital Beijing 100053, China.
authors: Hongxiao
authors: H
authors: Liu
authors: Department of Molecular Biology, Guanganmen Hospital China Academy of Traditional Chinese Medicine Beijing 100053, China.
authors: Min
authors: M
authors: Li
authors: Department of Molecular Biology, Guanganmen Hospital China Academy of Traditional Chinese Medicine Beijing 100053, China.
authors: Zhikui
authors: Z
authors: Wu
authors: Department of Rheumatology, Chinese Academy of Traditional Chinese Medicine, Guanganmen Hospital Beijing 100053, China.
authors: Xinghua
authors: X
authors: Feng
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006621:Hip Joint / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017746:Joint Capsule / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013167:Spondylitis, Ankylosing / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase; C056599:icariin
keywords: Ankylosing spondylitis; cytokine; fibroblast; icariin; ossification