title: Secretory factors of human chorion-derived stem cells enhance activation of human fibroblasts.
abstract: OBJECTIVE
  Wound healing remains a principal challenge in modern medical science. Chorion-dervied stem cells (CDSCs), isolated from human placenta, have largely been overlooked, and few studies on their potential in wound healing have been conducted. In this study, we investigated the functional characteristics of CDSCs compared with adipose-derived stem cells (ASCs) on human fibroblasts (HFs).
  
  
  METHODS
  We analyzed CDSCs by means of flow cytometry to confirm their mesenchymal stromal cell characteristics. We then evaluated the paracrine effects of CDSCs on HFs in a co-culture system and focused on fibroblast proliferation, migration and collagen synthesis. To explore the potential of CDSCs in wound healing, CDSC- and ASC-secreted factors were compared by use of a cytokine antibody array.
  
  
  RESULTS
  CDSCs had morphology similar to MSCs and expressed a mesenchymal stromal cell phenotype. HF proliferation and migration increased more than 5-fold when co-cultured with CDSCs. Furthermore, Western blot and reverse transcription-polymerase chain reaction analysis showed that expression of collagen (types I and III) in fibroblasts was upregulated 2-fold when co-cultured with CDSCs. Cytokine array results of CDSC-conditioned medium and ASC-conditioned medium revealed the presence of growth factors known to influence wound healing, including interleukin -6, interleukin -8, monocyte chemotactic protein 1 and regulated on activation, normal T cells expressed and secreted.
  
  
  CONCLUSIONS
  Our data demonstrated that CDSCs are functionally similar to ASCs, promote HF activation, and secrete growth factors that influence wound healing. Therefore, we suggest that CDSCs are potentially applicable in wound healing.
authors: Department of Molecular Biomedicine, The Catholic University of Korea, Seoul, Korea.
authors: Min Kyoung
authors: MK
authors: Kim
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors: Bommie F
authors: BF
authors: Seo
authors: Department of Molecular Biomedicine, The Catholic University of Korea, Seoul, Korea.
authors: Ki Joo
authors: KJ
authors: Kim
authors: Department of Molecular Biomedicine, The Catholic University of Korea, Seoul, Korea.
authors: Su-Jin
authors: SJ
authors: Lee
authors: Department of Molecular Biomedicine, The Catholic University of Korea, Seoul, Korea.
authors: Yeon Hee
authors: YH
authors: Ryu
authors: Department of Molecular Biomedicine, The Catholic University of Korea, Seoul, Korea; Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea. Electronic address: rhie@catholic.ac.kr.
authors: Jong Won
authors: JW
authors: Rhie
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology*; D002465:Cell Movement; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D002823:Chorion / Q000166:cytology*; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D014945:Wound Healing / Q000502:physiology*
chemical_list: C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D024042:Collagen Type I; D024061:Collagen Type III; D017077:Culture Media, Conditioned; C508600:IL6 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D016209:Interleukin-8
keywords: adipose-derived stem cells; chorion; fibroblasts; mesenchymal stromal cells; wound healing