title: With a little help from my friends: complementation as a survival strategy for viruses in a long-lived host system.
abstract: In selective host species, the extent of Citrus tristeza virus (CTV) infection is limited through the prevention of long-distance movement. As CTV infections often contain a population of multiple strains, we investigated whether the members of a population were capable of interaction, and what effect this would have on the infection process. We found that the tissue-tropism limitations of strain T36 in selective hosts could be overcome through interaction with a second strain, VT, increasing titer of, and number of cells infected by, T36. This interaction was strain-specific: other strains, T30 and T68, did not complement T36, indicating a requirement for compatibility between gene-products of the strains involved. This interaction was also host-specific, suggesting a second requirement of compatibility between the provided gene-product and host. These findings provide insight into the 'rules' that govern interaction between strains, and suggest an important mechanism by which viruses survive in a changing environment.
authors: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA. Electronic address: sjharper@ufl.edu.
authors: S J
authors: SJ
authors: Harper
authors: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors: S J
authors: SJ
authors: Cowell
authors: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors: W O
authors: WO
authors: Dawson
mesh_terms: D017790:Closterovirus / Q000235:genetics / Q000502:physiology*; D058507:Host Specificity; D054884:Host-Pathogen Interactions; D056265:Microbial Interactions; D010935:Plant Diseases / Q000821:virology; D019562:Viral Load; D056189:Viral Tropism
keywords: Citrus tristeza virus; Complementation; Movement; Phloem; Population