title: Increased expression of thymic stromal lymphopoietin and its receptor in Kimura's disease.
abstract: OBJECTIVE
  The objective of this study was to investigate the expression of thymic stromal lymphopoietin (TSLP) and TSLP receptor (TSLPR) in Kimura's disease (KD).
  
  
  METHODS
  Using parotid gland tissues from KD patients and control subjects, we quantified the expression levels of mRNA for TSLP, interleukin (IL)-25, IL-33, and their receptors by massively parallel sequencing. We also performed immunohistochemical analysis of TSLP and TSLPR, and counted cells immunoreactive for these proteins by the polymer immunocomplex and double immunofluorescence methods.
  
  
  RESULTS
  The levels of mRNA for TSLP, TSLPR, and IL-25R, but not IL-25, IL-33, or IL-33R, were significantly elevated in parotid gland tissues from the KD group as compared to the control group. Immunohistochemical analysis revealed that TSLP- and TSLPR-positive cells were significantly increased in number in parotid gland tissues from KD patients. Double immunofluorescence staining showed that TSLP and TSLPR were localized mainly in CD68-positive macrophages and tryptase-positive mast cells, respectively.
  
  
  CONCLUSIONS
  Overexpression of TSLP and TSLPR might contribute to the pathogenesis of KD through interactions between macrophages and mast cells. Regulation of TSLP/TSLPR signaling may be a potential therapeutic approach for KD.
authors: Department of Otolaryngology, Tokyo Women's Medical University, Tokyo, Japan.
authors: Eri
authors: E
authors: Sakitani
authors: Manabu
authors: M
authors: Nonaka
authors: Noriyuki
authors: N
authors: Shibata
authors: Toru
authors: T
authors: Furukawa
authors: Toshio
authors: T
authors: Yoshihara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000796:Angiolymphoid Hyperplasia with Eosinophilia / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult; D000094632:Thymic Stromal Lymphopoietin
chemical_list: C498762:CRLF2 protein, human; D016207:Cytokines; C486701:IL25 protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D000094632:Thymic Stromal Lymphopoietin