title: Total antioxidant capacity and lipid peroxidation in semen of patient with hyperviscosity.
abstract: Semen hyperviscosity (SHV) is one of the factors involved in deficiency in sperm function. This research aimed to evaluate seminal plasma total antioxidant capacity (TAC) and malondialdehyde (MDA) levels in infertile patients with hyperviscous and non-hyperviscous semen samples to understand whether hyperviscous semen is associated with oxidative damage in infertile subjects. In this cross sectional study, 59 semen samples were provided by fertile (n=12) individuals as control, infertile patients with normal viscosity (n=25) and infertile patients with hyperviscosity (n=22). After semen parameters examination, semen viscosity was studied by glass pipettes. Seminal plasma TAC and MDA levels were measured by ferric reducing of antioxidant power (FRAP) and thiobarbituric acid reaction (TBAR) methods, respectively. A probability less than 0.05 was considered statistically significant throughout the article. The mean of sperm parameters including: counts, motility and normal morphology in patients with hyperviscosity were significantly lower than those in non-hyperviscosity patients (p<0.05, p<0.01 and p<0.001, respectively). The mean of seminal plasma TAC value in seminal plasma of non-hyperviscosity patients (1710.31 ± 458.67 µmol/l) was significantly (p<0.01) higher than that of hyperviscosity group (1230.25 ± 352 µmol/l). A trend toward a higher mean of seminal plasma MDA value was estimated for hyperviscous group compared with non-hyperviscous (1.01 ± 0.41 nmol/ml vs. 0.94 ± 0.28 nmol/l); however, it was nonsignificant. Hyperviscous semen impairs seminal plasma TAC which is eventually associated with sperm membrane lipid peroxidation.
authors: Department of Biochemistry, Islamic Azad University, Sari Branch, Sari, Iran.
authors: Issa
authors: I
authors: Layali
authors: Member of Young Research Club, Islamic Azad University, Sari Branch, Sari, Iran.
authors: Eisa
authors: E
authors: Tahmasbpour
authors: Department of Psychology, Islamic Azad University, Sari Branch, Sari, Iran.
authors: Manijeh
authors: M
authors: Joulaei
authors: Fateme Zahra Infertility and Health Reproductive Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Seyed Gholam Ali
authors: SG
authors: Jorsaraei
authors: Department of Exercise Physiology, Islamic Azad University, Sari Branch, Sari, Iran.
authors: Parvin
authors: P
authors: Farzanegi
keywords: Antioxidants; Lipid Peroxidation; MDA; Male Infertility