title: Rapid Isolation of DNA from Staphylococcus.
abstract: Many methods exist to extract DNA from bacteria. Indeed, there is no shortage of kits available from manufacturers that allow for isolation of highly purified DNA. However, for many applications samples do not need to be extremely pure (i.e., free of contaminating proteins or RNA). Furthermore, for quick genetic screening, it is often useful to have a rapid and inexpensive option for DNA isolation from small samples. For these occasions, the method found in this chapter provides a cost-efficient, yet rapid, isolation of DNA.
authors: Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.
authors: Kelsey L
authors: KL
authors: Krausz
authors: Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA. jbose@kumc.edu.
authors: Jeffrey L
authors: JL
authors: Bose
mesh_terms: D002725:Chloroform / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D019800:Phenol / Q000737:chemistry; D013210:Staphylococcus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D019800:Phenol; D002725:Chloroform
keywords: DNA extraction; DNA isolation; DNA purification; Phenol–chloroform; Staphylococcus DNA