title: Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery.
abstract: A methacrylation strategy was employed to functionalize hyaluronic acid and prepare hyaluronic acid (HA) nanogels. Dynamic light scattering, zeta potential analyzer and electron microscopy were utilized to characterize the nanogels and their enzyme-degradability in vitro. It was found that these nanogels had a spherical morphology with the diameter of about 70nm, and negative surface potential. When doxorubicin (DOX) was loaded into the nanogels, the diameter decreased to approximately 50nm with a drug loading content of 16% and encapsulation efficiency of 62%. Cellular uptake examinations showed that HA nanogels could be preferentially internalized by two-dimensional (2D) cells and three-dimensional (3D) multicellular spheroids (MCs) which both overexpress CD44 receptor. Near-infrared fluorescence imaging, biodistribution and penetration examinations in tumor tissue indicated that the HA nanogels could efficiently accumulate and penetrate the tumor matrix. In vivo antitumor evaluation found that DOX-loaded HA nanogels exhibited a significantly superior antitumor effect.
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Chenchen
authors: C
authors: Yang
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Xin
authors: X
authors: Wang
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Xikuang
authors: X
authors: Yao
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Yajun
authors: Y
authors: Zhang
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Wei
authors: W
authors: Wu
authors: MOE Key Laboratory of High Performance Polymer Materials and Technology, Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing 210093, PR China. Electronic address: jiangx@nju.edu.cn.
authors: Xiqun
authors: X
authors: Jiang
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004337:Drug Carriers; D005782:Gels; D056945:Hep G2 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D050997:Nanomedicine; D053758:Nanoparticles; D010316:Particle Size; D012542:Scattering, Radiation; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D018874:Spheroids, Cellular; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; C497483:CD44 protein, human; C476399:Cd44 protein, mouse; D004337:Drug Carriers; D005782:Gels; D018960:Hyaluronan Receptors; D004317:Doxorubicin; D006820:Hyaluronic Acid; D008049:Lipase; D006821:Hyaluronoglucosaminidase
keywords: Drug delivery; Multicellular spheroids; Nanogel hyaluronic acid