title: Deciphering the role of the type II glyoxalase isoenzyme YcbL (GlxII-2) in Escherichia coli.
abstract: In Escherichia coli, detoxification of methylglyoxal (MG) requires glyoxalases I and II. Glyoxalase I (gloA/GlxI) isomerizes the hemithioacetal, formed spontaneously from MG and glutathione (GSH) to S-lactoylglutathione (SLG), which is hydrolyzed by glyoxalase II (gloB/GlxII) to lactate and GSH. YcbL from Salmonella enterica serovar Typhimurium is an unusual type II glyoxalase whose role in MG detoxification has remained enigmatic. Here we show that YcbL (gloC/GlxII-2) acts as an accessory type II glyoxylase in E. coli. The two isoenzymes have additive effects and ensure maximal MG degradation.
authors: Munich Center for integrated Protein Science (CiPSM) at the Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2-4, D-82152 Martinsried, Germany Institute of Environmental Medicine, UNIKA-T, Neusässer Straße 47, 86156 Augsburg, Germany Faculty of Medicine, Technische Universität München, Ismaningerstr. 22, 81675 Munich, Germany.
authors: Matthias
authors: M
authors: Reiger
authors: Munich Center for integrated Protein Science (CiPSM) at the Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.
authors: Jürgen
authors: J
authors: Lassak
authors: Munich Center for integrated Protein Science (CiPSM) at the Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Großhaderner Straße 2-4, D-82152 Martinsried, Germany jung@lmu.de.
authors: Kirsten
authors: K
authors: Jung
mesh_terms: D019295:Computational Biology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010802:Phylogeny; D011765:Pyruvaldehyde / Q000378:metabolism* / Q000494:pharmacology; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology / Q000235:genetics; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D005978:Glutathione; D007527:Isoenzymes; D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase; C013585:S-lactoylglutathione
keywords: VIBHAR_02708; VIBHAR_03213; Vibrio campbellii; gloB; methylglyoxal