title: N-Octanoyl Dopamine for Donor Treatment in a Brain-death Model of Kidney and Heart Transplantation.
abstract: BACKGROUND
  This study investigated the potential use of N-octanoyl dopamine (NOD) in donor management to ameliorate the damage caused by brain death and ischemia-reperfusion injury in a rat model of kidney and heart transplantation.
  
  
  METHODS
  Brain-dead Fisher rats were treated for 6 hours with either saline or saline plus NOD. Orthotopic kidney and heterotopic heart transplantation were performed in different Lewis recipient rats. The right donor kidneys were stored for biochemical analysis. Blood samples were taken from the donor and on several days after transplantation from the recipient. All grafts were harvested after 7 days.
  
  
  RESULTS
  There was no effect on donor heart rate and blood pressure under NOD treatment. The release of lactate dehydrogenase (LDH) during brain death was reduced in the NOD group. The right kidneys from NOD-preconditioned animals revealed diminished expression of the proinflammatory cell adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1). Nevertheless, there was no difference in renal infiltration with ED1 (CD68) or major histocompatibility complex (MHC) class II-positive cells. Recipients receiving a renal allograft from NOD-treated donors had a significantly better renal function at day 1 after transplantation. Banff-grading after 7 days showed significantly reduced scores for tubulitis and vasculitis in the grafts of these recipients. In the heart allograft recipients, lower plasma LDH levels were observed.
  
  
  CONCLUSIONS
  Donor preconditioning with NOD leads to better graft function and reduced acute rejection in untreated renal allograft recipients without displaying adverse effects on heart allografts.
authors: 1 5th Department of Medicine, University Medical Centre Mannheim, Heidelberg University, Germany. 2 Herz- und Nierenzentrum Weinheim, Germany. 3 Novaliq GmbH, Heidelberg, Germany. 4 Department of Anesthesiology, University Medical Centre Mannheim, Heidelberg University, Heidelberg, Germany.
authors: Rahel S
authors: RS
authors: Spindler
authors: Peter
authors: P
authors: Schnuelle
authors: Lukas
authors: L
authors: Nickels
authors: Jonas
authors: J
authors: Jarczyk
authors: Rüdiger
authors: R
authors: Waldherr
authors: Sonja
authors: S
authors: Theisinger
authors: Bastian
authors: B
authors: Theisinger
authors: Sarah
authors: S
authors: Klotz
authors: Charalambos
authors: C
authors: Tsagogiorgas
authors: Uwe
authors: U
authors: Göttmann
authors: Bernhard K
authors: BK
authors: Krämer
authors: Benito A
authors: BA
authors: Yard
authors: Simone
authors: S
authors: Hoeger
mesh_terms: D000818:Animals; D001926:Brain Death; D004298:Dopamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016027:Heart Transplantation; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation; D008297:Male; D009206:Myocardium / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D050916:TRPV Cation Channels / Q000502:physiology; D014019:Tissue Donors
chemical_list: C554838:N-octanoyldopamine; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D004298:Dopamine