title: Clinical significance of chromosome 1p/19q loss of heterozygosity and Sox17 expression in oligodendrogliomas.
abstract: OBJECTIVE
  To study chromosome 1p/19q loss of heterozygosity (LOH) and Sox17 protein expression in oligodendrogliomas and correlate this loss with clinicopathological features.
  
  
  METHODS
  This study included 100 cases of oligodendrogliomas at the First Affiliated Hospital of Xinjiang Medical University from 2003 to 2014. The cases included paraffin-embedded tissues from 50 low-grade oligodendrogliomas and 50 anaplastic oligodendrogliomas. Chromosome 1p/19q LOH was detected by fluorescence in situ hybridization (FISH) and Sox17 protein expression was analyzed by immunohistochemistry. Clinicopathological characteristics of the oligodendrogliomas were compared and prognosis analyzed using Cox regression and Kaplan-Meier analyses.
  
  
  RESULTS
  The LOH positivity rate of 1p/19q was 52% in 50 cases of low-grade oligodendrogliomas and 68% in 50 cases of anaplastic oligodendrogliomas (P = 0.102). The rates of Sox17 expression were significantly different in oligodendrogliomas (82%) and anaplastic oligodendrogliomas (62%, P = 0.026). Single factor analysis determined that 1p/19q LOH (P = 0.000), Sox17 protein expression (P = 0.000), location (P = 0.001), chemotherapy (P = 0.000), and radiation therapy (P = 0.001) were associated with oligodendroglioma patient prognosis. Cox multiple factors regression analysis determined that 1p/19q LOH and Sox17 expression were independent prognostic factors of oligodendrogliomas.
  
  
  CONCLUSIONS
  In this study, oligodendroglioma patients with 1p/19q LOH and Sox17 protein expression had a better prognosis. Thus, analysis of 1p/19q LOH and Sox17 protein expression could significantly enhance diagnostic accuracy, guide treatment, and improve the prognosis.
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Junzhi
authors: J
authors: Li
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Na
authors: N
authors: Miao
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Ming
authors: M
authors: Liu
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Wenli
authors: W
authors: Cui
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Xia
authors: X
authors: Liu
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Xinxia
authors: X
authors: Li
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Xiaoli
authors: X
authors: Shi
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Song
authors: S
authors: Qing
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Yuqing
authors: Y
authors: Ma
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Wei
authors: W
authors: Zhang
authors: Department of Pathology, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uyger Automatic Region, Urumqi 830054, China.
authors: Hadeti
authors: H
authors: Biekemituofu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality; D002648:Child; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009837:Oligodendroglioma / Q000235:genetics / Q000378:metabolism / Q000401:mortality; D011379:Prognosis; D016016:Proportional Hazards Models; D055760:SOXF Transcription Factors / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C109188:SOX17 protein, human; D055760:SOXF Transcription Factors
keywords: 1p/19q LOH; Oligodendroglioma; Sox17; clinical features; prognosis