title: Quercetin loaded biopolymeric colloidal particles prepared by simultaneous precipitation of quercetin with hydrophobic protein in aqueous medium.
abstract: Quercetin loaded biopolymeric colloidal particles were prepared by precipitating quercetin (water insoluble polyphenol) and zein (hydrophobic protein), simultaneously, by adding their hydro-alcoholic solution to aqueous solution in presence of sodium caseinate as an electrosteric stabiliser. The presence of protein resulted in altering the shape of quercetin precipitates from needle-like to spherical shape at higher zein proportions, as confirmed by transmission electron microscopy. The average particle size of zein:quercetin composite particles was below 200 nm (130-161 nm) with negative surface charge (-30 to -41 mV), as confirmed by dynamic light scattering and electrophoretic mobility data. Solid state characterisation (X-ray diffraction) and spectroscopic measurements (UV-Vis and IR spectroscopy) confirmed characteristic changes in quercetin due to the entrapment in the biopolymeric matrix of colloidal particles. Results from anti-oxidant study demonstrated the advantage of entrapping quercetin in the colloidal particles in terms of the chemical stability in the alkaline pH and against photodegradation under UV-light irradiation.
authors: Unilever R&D, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands. Electronic address: Ashok.Patel@Unilever.com.
authors: Ashok R
authors: AR
authors: Patel
authors: Unilever R&D, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands.
authors: Patricia C M
authors: PC
authors: Heussen
authors: Unilever R&D, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands.
authors: Johan
authors: J
authors: Hazekamp
authors: Unilever R&D, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands.
authors: Ellen
authors: E
authors: Drost
authors: Unilever R&D, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands. Electronic address: Krassimir.Velikov@Unilever.com.
authors: Krassimir P
authors: KP
authors: Velikov
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002364:Caseins / Q000737:chemistry; D011232:Chemical Precipitation; D003102:Colloids / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D011794:Quercetin / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D015028:Zein / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002364:Caseins; D003102:Colloids; D014867:Water; D015028:Zein; D011794:Quercetin
keywords: Anti-oxidant activity; Electrosteric stabilisation; Quercetin; Zein colloidal particles