title: Molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in Africa.
abstract: Knowledge of Kobuvirus (Family Picornaviridae) infection in carnivores is limited and has not been described in domestic or wild carnivores in Africa. To fill this gap in knowledge we used RT-PCR to screen fresh feces from several African carnivores. We detected kobuvirus RNA in samples from domestic dog, golden jackal, side-striped jackal and spotted hyena. Using next generation sequencing we obtained one complete Kobuvirus genome sequence from each of these species. Our phylogenetic analyses revealed canine kobuvirus (CaKV) infection in all four species and placed CaKVs from Africa together and separately from CaKVs from elsewhere. Wild carnivore strains were more closely related to each other than to those from domestic dogs. We found that the secondary structure model of the IRES was similar to the Aichivirus-like IRES subclass and was conserved among African strains. We describe the first CaKVs from Africa and extend the known host range of CaKV.
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany.
authors: Ximena A
authors: XA
authors: Olarte-Castillo
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany; Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Straße 6-8, 14195 Berlin, Germany.
authors: Felix
authors: F
authors: Heeger
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany; Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Straße 6-8, 14195 Berlin, Germany.
authors: Camila J
authors: CJ
authors: Mazzoni
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany.
authors: Alex D
authors: AD
authors: Greenwood
authors: Tanzania Wildlife Research Institute, P.O. Box 661, Arusha, Tanzania.
authors: Robert
authors: R
authors: Fyumagwa
authors: EcoHealth Alliance, 460 West 34th Street, New York, NY, USA.
authors: Patricia D
authors: PD
authors: Moehlman
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany.
authors: Heribert
authors: H
authors: Hofer
authors: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany. Electronic address: east@izw-berlin.de.
authors: Marion L
authors: ML
authors: East
mesh_terms: D000349:Africa; D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D002335:Carnivora; D016000:Cluster Analysis; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D052699:Kobuvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral
keywords: Domestic dog; Golden jackal; IRES; Kobuvirus; Next-generation sequencing; ORF; Phylogeny; Side-striped jackal; Spotted hyena; Whole genome