title: Improved biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by adding L-glutamine plus glycine instead of NAD+ in β-cyclodextrin-water system.
abstract: To reduce dependence on the expensive cofactor and effectively biotransform ethyl 4-chloro-3-oxobutanoate, L-glutamine and glycine were found to enhance the content of intracellular NADH and the reductase activity. Adding the mixture of 200 mM of L-glutamine and 500 mM of glycine to the reaction media, a 1.67-fold of reductase activity was increased over the control without the addition of the two compounds. Moreover, β-cyclodextrin (0.4 mol β-cyclodextrin/mol ethyl 4-chloro-3-oxobutanoate) was also added into this reaction media, and the biocatalytic activity of the whole-cell biocatalyst of Escherichia coli CCZU-K14 was increased by 1.34-fold than that without β-cyclodextrin. In this β-cyclodextrin-water media containing L-glutamine (200 mM) plus glycine (500 mM), ethyl (S)-4-chloro-3-hydroxybutanoate (>99% ee) could be obtained from 3000 mM ethyl 4-chloro-3-oxobutanoate in the yield of 98.0% after 8h. All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of ethyl (S)-4-chloro-3-hydroxybutanoate.
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. Electronic address: heyucai2001@163.com.
authors: Yu-Cai
authors: YC
authors: He
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Dan-Ping
authors: DP
authors: Zhang
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Zhi-Cheng
authors: ZC
authors: Tao
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Yun
authors: Y
authors: Lu
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Yun
authors: Y
authors: Ding
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Feng
authors: F
authors: Liu
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Zheng-Zhong
authors: ZZ
authors: Zhu
authors: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors: Huan
authors: H
authors: Rui
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors: Gao-Wei
authors: GW
authors: Zheng
authors: Department of Bioengineering, Rice University, Houston, TX, USA.
authors: Xian
authors: X
authors: Zhang
mesh_terms: D000090:Acetoacetates / Q000737:chemistry / Q000378:metabolism; D002087:Butyrates / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry / Q000378:metabolism*; D005998:Glycine / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000090:Acetoacetates; D002087:Butyrates; D047392:beta-Cyclodextrins; C121244:ethyl 4-chloro-3-hydroxybutanoate; D014867:Water; D005973:Glutamine; D009243:NAD; C111982:ethyl 4-chloro-3-oxobutanoate; C031215:betadex; D005998:Glycine
keywords: Ethyl (S)-4-chloro-3-hydroxybutanoate; Ethyl 4-chloro-3-oxobutanoate; Glycine; l-Glutamine; β-Cyclodextrin