title: Classification of malignant and benign tumors of the lung by infrared spectral histopathology (SHP).
abstract: We report results of a study utilizing a novel tissue classification method, based on label-free spectral techniques, for the classification of lung cancer histopathological samples on a tissue microarray. The spectral diagnostic method allows reproducible and objective classification of unstained tissue sections. This is accomplished by acquiring infrared data sets containing thousands of spectra, each collected from tissue pixels ∼6 μm on edge; these pixel spectra contain an encoded snapshot of the entire biochemical composition of the pixel area. The hyperspectral data sets are subsequently decoded by methods of multivariate analysis that reveal changes in the biochemical composition between tissue types, and between various stages and states of disease. In this study, a detailed comparison between classical and spectral histopathology is presented, suggesting that spectral histopathology can achieve levels of diagnostic accuracy that is comparable to that of multipanel immunohistochemistry.
authors: Department of Pathology, University of Massachusetts Medical School, Worcester, MA, USA.
authors: Ali
authors: A
authors: Akalin
authors: 1] Department of Mathematics and Statistics and Program in Bioinformatics, Boston University, Boston, MA, USA [2] Cireca Theranostics, LLC, Cambridge, MA, USA.
authors: Xinying
authors: X
authors: Mu
authors: Department of Mathematics and Statistics and Program in Bioinformatics, Boston University, Boston, MA, USA.
authors: Mark A
authors: MA
authors: Kon
authors: Cireca Theranostics, LLC, Cambridge, MA, USA.
authors: Ayşegül
authors: A
authors: Ergin
authors: Cireca Theranostics, LLC, Cambridge, MA, USA.
authors: Stan H
authors: SH
authors: Remiszewski
authors: 1] Cireca Theranostics, LLC, Cambridge, MA, USA [2] Creative Creek, LLC, Camano Island, WA, USA.
authors: Clay M
authors: CM
authors: Thompson
authors: Division of Thoracic Surgery, City of Hope Medical Center, Duarte, CA, USA.
authors: Dan J
authors: DJ
authors: Raz
authors: 1] Cireca Theranostics, LLC, Cambridge, MA, USA [2] Laboratory for Spectral Diagnosis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
authors: Max
authors: M
authors: Diem
authors: Benjamin
authors: B
authors: Bird
authors: Miloš
authors: M
authors: Miljković
mesh_terms: D006652:Histological Techniques / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000473:pathology*; D015999:Multivariate Analysis; D013055:Spectrophotometry, Infrared / Q000379:methods*; D046888:Tissue Array Analysis / Q000379:methods*