title: The CHEK2 1100delC allelic variant is not present in familial and sporadic breast cancer cases from Moroccan population.
abstract: OBJECTIVE
  The cell-cycle checkpoint kinase 2 (CHEK2) is an important signal transducer of cellular responses to DNA damage, whose defects has been associated with increased risk for breast cancer. The CHEK2 1100delC mutation has been reported to confer a twofold increased risk of breast cancer among carriers. The frequency of the mutation varies among populations. The highest frequency has been described in Northern and Eastern European countries. However, the 1100delC mutation has been investigated in different case-control studies and none in Moroccan population. The aim of this study was to evaluate the prevalence of this variant and determine its contribution to the development of breast cancer in sporadic cases and also in members of breast cancer families who tested negative or positive for a deleterious mutation in BRCA1/BRCA2.
  
  
  METHODS
  In this case-control study we performed the CHEK2 1100delC mutation analysis by ASO-PCR in 134 breast cancer patients and 114 unaffected control individuals. Most of these families had several cases of breast cancer or ovarian cancer (or both).
  
  
  RESULTS
  No CHEK2 1100delC mutations were detected in any of 134 individuals, including 59 women diagnosed with breast cancer at an early age (<40 years), 10 women with bilateral breast cancer, and 6 women with ovarian cancer.
  
  
  CONCLUSIONS
  Our preliminary genetic analysis are consistent with the reported very low frequency of CHEK2 1100delC mutation in North American populations (compared with Northern Europe), rendering CHEK2 1100delC such as an unlikely to be major breast cancer susceptibility genes.
authors: Laboratory of Genetics and Molecular Pathology-Medical School of Casablanca, 19 rue Tarik Ibn Ziad, P.C 9154 Casablanca, Morocco ; University Hassan II Ain Chock, Center Of Doctoral Sciences "In Health Sciences", Casablanca, Morocco.
authors: Chaymaa
authors: C
authors: Marouf
authors: Department of Oncology, Littoral Clinic, Casablanca, Morocco.
authors: Omar
authors: O
authors: Hajji
authors: Laboratory of Genetics and Molecular Pathology-Medical School of Casablanca, 19 rue Tarik Ibn Ziad, P.C 9154 Casablanca, Morocco ; University Hassan II Ain Chock, Center Of Doctoral Sciences "In Health Sciences", Casablanca, Morocco.
authors: Brehima
authors: B
authors: Diakité
authors: Laboratory of Genetics and Molecular Pathology-Medical School of Casablanca, 19 rue Tarik Ibn Ziad, P.C 9154 Casablanca, Morocco ; University Hassan II Ain Chock, Center Of Doctoral Sciences "In Health Sciences", Casablanca, Morocco.
authors: Amal
authors: A
authors: Tazzite
authors: Mohammed VI Center for Cancer Treatment, Ibn Rochd University Hospital, Casablanca, Morocco.
authors: Hassan
authors: H
authors: Jouhadi
authors: Mohammed VI Center for Cancer Treatment, Ibn Rochd University Hospital, Casablanca, Morocco.
authors: Abdellatif
authors: A
authors: Benider
authors: Laboratory of Genetics and Molecular Pathology-Medical School of Casablanca, 19 rue Tarik Ibn Ziad, P.C 9154 Casablanca, Morocco ; University Hassan II Ain Chock, Center Of Doctoral Sciences "In Health Sciences", Casablanca, Morocco.
authors: Sellama
authors: S
authors: Nadifi