title: The macrophage galactose-type lectin-1 (MGL1) recognizes Taenia crassiceps antigens, triggers intracellular signaling, and is critical for resistance to this infection.
abstract: C-type lectins are multifunctional sugar-binding molecules expressed on dendritic cells (DCs) and macrophages that internalize antigens for processing and presentation. Macrophage galactose-type lectin 1 (MGL1) recognizes glycoconjugates expressing Lewis X structures which contain galactose residues, and it is selectively expressed on immature DCs and macrophages. Helminth parasites contain large amounts of glycosylated components, which play a role in the immune regulation induced by such infections. Macrophages from MGL1(-/-) mice showed less binding ability toward parasite antigens than their wild-type (WT) counterparts. Exposure of WT macrophages to T. crassiceps antigens triggered tyrosine phosphorylation signaling activity, which was diminished in MGL1(-/-) macrophages. Following T. crassiceps infection, MGL1(-/-) mice failed to produce significant levels of inflammatory cytokines early in the infection compared to WT mice. In contrast, MGL1(-/-) mice developed a Th2-dominant immune response that was associated with significantly higher parasite loads, whereas WT mice were resistant. Flow cytometry and RT-PCR analyses showed overexpression of the mannose receptors, IL-4Rα, PDL2, arginase-1, Ym1, and RELM-α on MGL1(-/-) macrophages. These studies indicate that MGL1 is involved in T. crassiceps recognition and subsequent innate immune activation and resistance.
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Daniel
authors: D
authors: Montero-Barrera
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Héctor
authors: H
authors: Valderrama-Carvajal
authors: Department of Pathology, The Ohio State University Medical Center, Columbus, OH, USA.
authors: César A
authors: CA
authors: Terrazas
authors: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
authors: Saúl
authors: S
authors: Rojas-Hernández
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Yadira
authors: Y
authors: Ledesma-Soto
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Laura
authors: L
authors: Vera-Arias
authors: Proyecto CyMA, UIICSE, FES Iztacala, UNAM, 54090 Tlalnepantla, MEX, Mexico.
authors: Maricela
authors: M
authors: Carrasco-Yépez
authors: Instituto Nacional de Cardiología "Ignacio Chávez," 14080 Mexico, DF, Mexico.
authors: Lorena
authors: L
authors: Gómez-García
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Diana
authors: D
authors: Martínez-Saucedo
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Mireya
authors: M
authors: Becerra-Díaz
authors: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), Avenue de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors: Luis I
authors: LI
authors: Terrazas
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001212:Asialoglycoproteins / Q000172:deficiency / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis; D060467:Disease Resistance / Q000276:immunology*; D005260:Female; D005690:Galactose / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism; D007109:Immunity; D042541:Intracellular Space / Q000378:metabolism; D007700:Kinetics; D037181:Lectins, C-Type / Q000172:deficiency / Q000378:metabolism*; D008262:Macrophage Activation / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008565:Membrane Proteins / Q000172:deficiency / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D015398:Signal Transduction; D012995:Solubility; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D001212:Asialoglycoproteins; C064720:Clec10a protein, mouse; D016207:Cytokines; D006001:Glycoconjugates; D037181:Lectins, C-Type; D008565:Membrane Proteins; D019000:Phosphotyrosine; D000116:Acetylgalactosamine; D005690:Galactose