title: Involvement of nitric oxide and caveolins in the age-associated functional and structural changes in a heart under osmotic stress.
abstract: Previous work done in our laboratory showed that water restriction during 24 and 72h induced changes in cardiovascular NOS activity without altering NOS protein levels in young and adult animals. These findings indicate that the involvement of NO in the regulatory mechanisms during dehydration depends on the magnitude of the water restriction and on age. Our aim was to study whether a controlled water restriction of 1 month affects cardiac function, NO synthase (NOS) activity and NOS, and cav-1 and -3 protein levels in rats during aging. Male Sprague-Dawley rats aged 2 and 16 months were divided into 2 groups: (CR) control restriction (WR) water restriction. Measurements of arterial blood pressure, heart rate, oxidative stress, NOS activity and NOS/cav-1 and -3 protein levels were performed. Cardiac function was evaluated by echocardiography. The results showed that adult rats have greater ESV, EDV and SV than young rats with similar SBP. Decreased atria NOS activity was caused by a reduction in NOS protein levels. Adult animals showed increased cav-1. Water restriction decreased NOS activity in young and adult rats associated to an increased cav-1. TBARS levels increased in adult animals. Higher ventricular NOS activity in adulthood would be caused by a reduction in both cav. Water restriction reduced NOS activity and increased cav in both age groups. In conclusion, our results indicated that dehydration modifies cardiac NO system activity and its regulatory proteins cav in order to maintain physiological cardiac function. Functional alterations are induced by the aging process as well as hypovolemic state.
authors: Department of Physiology, University of Buenos Aires, Buenos Aires, Argentina.
authors: Patricia
authors: P
authors: Arza
authors: Department of Physiology, University of Buenos Aires, Buenos Aires, Argentina.
authors: Vanina
authors: V
authors: Netti
authors: Department of Cardiology and Internal Medicine, Air Force Hospital, Buenos Aires, Argentina.
authors: Francisco
authors: F
authors: Perosi
authors: Department of Physiology, University of Buenos Aires, Buenos Aires, Argentina; Department of Cardiology and Internal Medicine, Air Force Hospital, Buenos Aires, Argentina.
authors: Gustavo
authors: G
authors: Cernadas
authors: Laboratory of Pathophysiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
authors: Federico
authors: F
authors: Ochoa
authors: Laboratory of Free Radical Biology (IBIMOL), School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors: Natalia
authors: N
authors: Magnani
authors: Laboratory of Free Radical Biology (IBIMOL), School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors: Pablo
authors: P
authors: Evelson
authors: Laboratory of Pathophysiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
authors: Elsa
authors: E
authors: Zotta
authors: Department of Physiology, University of Buenos Aires, Buenos Aires, Argentina.
authors: Andrea
authors: A
authors: Fellet
authors: Department of Physiology, University of Buenos Aires, Buenos Aires, Argentina. Electronic address: abalasz@ffyb.uba.ar.
authors: Ana M
authors: AM
authors: Balaszczuk
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D022461:Caveolins / Q000378:metabolism*; D004562:Electrocardiography; D005247:Feeding Behavior; D005355:Fibrosis; D006339:Heart Rate; D006352:Heart Ventricles / Q000378:metabolism; D006439:Hemodynamics; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009997:Osmotic Pressure; D017207:Rats, Sprague-Dawley; D013599:Systole; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014556:Urine
chemical_list: D022461:Caveolins; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase
keywords: Aging; Cardiac morphology; Caveolins; Heart nitric oxide; Water restriction