title: Genistein prevents ultraviolet B radiation-induced nitrosative skin injury and promotes cell proliferation.
abstract: Nitric oxide (NO) levels increase considerably after 24h of exposure of skin to ultraviolet B (UVB) radiation, which leads to nitrosative skin injury. In addition, increased NO levels after exposure to UVB radiation are associated with inhibition of cell proliferation. Compared to the UV-control group, UV-genistein at 10 mg/kg (UV-GEN10) group showed tissue protection, decreased lipid peroxide and nitrotyrosine formation, and low CAT activity. Furthermore, NO levels and iNOS labeling remained high. In this group, the reduction in lipid peroxides and nitrotyrosine was accompanied by upregulation of cell proliferation factors (Ki67 and PCNA), which indicated that prevention of nitrosative skin injury promoted cell proliferation and DNA repair. Genistein also prevented nitrosative events, inhibited ONOO(-) formation, which leads to tissue protection and cell proliferation. The UV-GEN15 group did not result in a greater protective effect compared to that with UV-GEN10 group. In the UV-GEN15 group, histological examination of the epidermis showed morphological alterations without efficient protection against lipid peroxide formation, as well as inhibition of Ki67 and PCNA, and VEGF labeling, which suggested inhibition of cell proliferation. These results help to elucidate the mechanisms underlying the photoprotective effect of genistein and reveal the importance of UVB radiation-induced nitrosative damage.
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: V A
authors: VA
authors: Terra
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil; Laboratorio de Patofisiologia e Radicais Livres, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: F P
authors: FP
authors: Souza-Neto
authors: Departamento de Clínica Medica, Divisão de Dermatologia, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: M A C
authors: MA
authors: Frade
authors: Departamento de Patologia e Medicina Legal, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: L N Z
authors: LN
authors: Ramalho
authors: Departamento de Clínica Medica, Divisão de Dermatologia, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: T A M
authors: TA
authors: Andrade
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: A A C
authors: AA
authors: Pasta
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: A C
authors: AC
authors: Conchon
authors: Departamento de Patologia e Medicina Legal, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: F A
authors: FA
authors: Guedes
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: R C
authors: RC
authors: Luiz
authors: Laboratorio de Patofisiologia e Radicais Livres, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil.
authors: R
authors: R
authors: Cecchini
authors: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil; Laboratorio de Patofisiologia e Radicais Livres, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, PR, Brazil. Electronic address: alcecchini@uel.br.
authors: A L
authors: AL
authors: Cecchini
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D019833:Genistein / Q000494:pharmacology*; D019394:Ki-67 Antigen / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A; D019833:Genistein