title: Identification of peptide regions of SERPINA1 and ENOSF1 and their protein expression as potential serum biomarkers for gastric cancer.
abstract: This study aimed to detect potential serum biomarkers for gastric cancer. In the present study, we used magnetic bead-based purification and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to detect potential serum markers in 70 gastric cancer (GC) patients compared with 72 healthy controls. On average, up to 81 peaks, of which 11 were significantly different m/z peaks (fold change >1.5; P < 0.001, Wilcoxon rank sum test) between GC group and healthy controls were detected. Two potential gastric serum biomarkers (m/z values of 1546.02 and 5335.08), with higher and specific expression in GC patients were further identified as peptide regions of SERPINA1 and ENOSF1. Enzyme-linked immunosorbent assays (ELISAs) were used to analyze 210 additional serum samples obtained from 36 healthy volunteers, 36 GC patients, 30 GU patients, 36 nonsmall-cell lung cancer (NSCLC) patients, 36 clear-cell renal cell carcinoma (CCRCC) patients, and 36 pancreatic cancer patients to verify the expression of SERPINA1 and ENOSF1 in GC sera. The suitability of the present method for gastric serum proteomic analysis was demonstrated and led to the identification of two peptide regions and their corresponding proteins as potential serum biomarkers for the serum detection of GC.
authors: Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China, yangjuan0112@mail.xjtu.edu.cn.
authors: Juan
authors: J
authors: Yang
authors: Xiaofan
authors: X
authors: Xiong
authors: Xiaofei
authors: X
authors: Wang
authors: Bo
authors: B
authors: Guo
authors: Kang
authors: K
authors: He
authors: Chen
authors: C
authors: Huang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006836:Hydro-Lyases; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin; C108693:ENOSF1 protein, human; D006836:Hydro-Lyases