title: Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern.
abstract: A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1(T), was isolated from water of a saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1(T) grew in media containing 5-20% (w/v) salts (optimum 7.5% salts). It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 15-45 °C (optimally at 37 °C). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1(T) is a member of the genus Aquisalimonas . The closest relatives to this strain were Aquisalimonas halophila YIM 95345(T) and Aquisalimonas asiatica CG12(T) with sequence similarities of 99.4% and 97.0%, respectively. DNA-DNA hybridization between the novel isolate and Aquisalimonas halophila YIM 95345(T) revealed a relatedness of 54%. The major fatty acids of strain BA42AL-1(T) were C(18 : 1)ω6c/C(18 : 1)ω7c, C(19 : 0) cyclo ω8c and C(16 : 0), and lower contents of C12 : 0 and C18 : 0. The polar lipid pattern of strain BA42AL-1(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G+C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA-DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA42AL-1(T) is proposed as a novel species of the genus Aquisalimonas , for which the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1(T) ( = CCM 8472(T) = CECT 8326(T) = LMG 27614(T)).
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors: Carmen
authors: C
authors: Infante-Domínguez
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors: Cristina
authors: C
authors: Sánchez-Porro
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors: Antonio
authors: A
authors: Ventosa
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D042001:Ectothiorhodospiraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D013030:Spain; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S