title: Actinomadura darangshiensis sp. nov., isolated from a volcanic cone.
abstract: An actinobacterium, designated strain DLS-70(T), was isolated from a soil sample from the surface of a rock on the peak of Darangshi Oreum (a volcanic cone) in Jeju, Republic of Korea. Substrate mycelium was produced abundantly on most of the media tested. Spiral chains of spores with warty surface developed on the aerial hyphae. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DLS-70(T) belonged to the genus Actinomadura and was most closely related to Actinomadura bangladeshensis DSM 45347(T) (98.9% sequence similarity), Actinomadura madurae DSM 43067(T) (98.8%) and Actinomadura chokoriensis DSM 45346(T) (98.6%). Chemotaxonomic observations supported the assignment of the isolate to the genus Actinomadura . DNA-DNA relatedness values between strain DLS-70(T) and the type strains of the closest phylogenetic relatives were less than 20%. On the basis of data from this polyphasic study, strain DLS-70(T) ( =KCTC 29224(T) =DSM 45941(T)) is considered to represent a novel species of the genus Actinomadura , for which the name Actinomadura darangshiensis sp. nov. is proposed.
authors: Department of Science Education, Educational Science Research Institute, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Soon Dong
authors: SD
authors: Lee
authors: Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Deajeon 305-764, Republic of Korea.
authors: Seung Bum
authors: SB
authors: Kim
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D018707:Volcanic Eruptions
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid