title: Multidimensional microchip-capillary electrophoresis device for determination of functional proteins in infant milk formula.
abstract: Functional proteins have been found in infant milk formula as supplements, added by an increasing number of manufacturers. Their supplementations are expected to be controlled and monitored. Here, we describe a microchip-integrated CE method for the determination of these low levels of functional proteins in a protein-rich sample matrix. On-chip isoelectric focusing (IEF) is used to separate high-abundance proteins from low-abundance proteins instead of using some complicated time-consuming protein purification process. After that, transient isotachophoresis hyphenated capillary zone electrophoresis (t-ITP-CZE) can preconcentrate, separate, and analyze transferred functional proteins in the embedded capillary under UV detection.
authors: Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore, Singapore, 638075, rgwu@SIMTech.a-star.edu.sg.
authors: Ruige
authors: R
authors: Wu
authors: Zhiping
authors: Z
authors: Wang
authors: Ying Sing
authors: YS
authors: Fung
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins