title: Performance of an HRP-2 rapid diagnostic test in Nigerian children less than 5 years of age.
abstract: The diagnostic performance of histidine-rich protein 2 (HRP-2)-based malaria rapid diagnostic test (RDT) was evaluated in a mesoendemic area for malaria, Kaduna, Nigeria. We compared RDT results with expert microscopy results of blood samples from 295 febrile children under 5 years. Overall, 11.9% (35/295) tested positive with RDT compared with 10.5% (31/295) by microscopy: sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 100%, 98.5%, 88.6%, and 100%, respectively. The RDT sensitivity was not affected by transmission season, parasite density, and age. Specificity and positive PV decreased slightly during the high-transmission season (97.5% and 83.3%). The RDT test positivity rates in the low- and high-transmission seasons were 9.4% and 13.5%, respectively. Overall, the test performance of this RDT was satisfactory. The findings of a low proportion of RDT false positives, no invalid and no false-negative results should validate the performance of RDTs in this context.
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia femiajumobi@gmail.com.
authors.forename: Olufemi
authors.initials: O
authors.lastname: Ajumobi
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Kabir
authors.initials: K
authors.lastname: Sabitu
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Patrick
authors.initials: P
authors.lastname: Nguku
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Jacob
authors.initials: J
authors.lastname: Kwaga
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Godwin
authors.initials: G
authors.lastname: Ntadom
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Sheba
authors.initials: S
authors.lastname: Gitta
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Rutebemberwa
authors.initials: R
authors.lastname: Elizeus
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Wellington
authors.initials: W
authors.lastname: Oyibo
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Peter
authors.initials: P
authors.lastname: Nsubuga
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Mark
authors.initials: M
authors.lastname: Maire
authors.affiliation: Nigeria Field Epidemiology and Laboratory Training Programme, Abuja, Nigeria; Ahmadu Bello University, Zaria, Nigeria; National Malaria Control Programme, Federal Ministry of Health, Abuja, Nigeria; African Field Epidemiology Network, Kampala, Uganda; Makerere University, Kampala, Uganda; College of Medicine, University of Lagos, Nigeria; Global Public Health Solutions, Atlanta, Georgia; Division of Global Health Protection and Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Poggensee
mesh_terms: D000953:Antigens, Protozoan / Q000097:blood*; D002675:Child, Preschool; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D005334:Fever; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D009549:Nigeria; D018512:Parasitemia; D010963:Plasmodium falciparum / Q000276:immunology* / Q000302:isolation & purification; D011237:Predictive Value of Tests; D015800:Protozoan Proteins / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012621:Seasons; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D015800:Protozoan Proteins; D011933:Reagent Kits, Diagnostic