title: Insight into conformational modification of alpha-synuclein in the presence of neuronal whole cells and of their isolated membranes.
abstract: A change in the conformational plasticity of α-Synuclein (α-Syn) is hypothesised to be a key step in the pathogenic mechanism of Parkinson's disease (PD). Here, we report the study of extracellular α-Syn interaction with whole cells and membranes isolated from the neuronal SH-SY5Y cells, exploiting NMR and CD spectroscopies. In addition, the crosslinking agent DSG was used to freeze the conformational and oligomeric state of α-Syn in the presence of cells. These data, in a quasi-physiological environment, confirm the protein monomeric state with a propensity to adopt a transient alpha helical following interaction with biological membranes.
authors.affiliation: Istituto di Biostrutture e Bioimmagini, C.N.R. via Mezzocannone, 16, 80134 Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Smaldone
authors.affiliation: Istituto di Biostrutture e Bioimmagini, C.N.R. via Mezzocannone, 16, 80134 Napoli, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Diana
authors.affiliation: DBSV - Dept. of Biotechnology and Life Sciences - Università degli Studi dell'Insubria, via J.H. Dunant 3, 21100 Varese, Italy; The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Politecnico di Milano, ICRM CNR Milano, Università degli studi dell'Insubria, Milano, Italy.
authors.forename: Loredano
authors.initials: L
authors.lastname: Pollegioni
authors.affiliation: Istituto di Biostrutture e Bioimmagini, C.N.R. via Mezzocannone, 16, 80134 Napoli, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Di Gaetano
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli via Vivaldi, 43, 81100 Caserta, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Fattorusso
authors.affiliation: Istituto di Biostrutture e Bioimmagini, C.N.R. via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: empedone@unina.it.
authors.forename: Emilia
authors.initials: E
authors.lastname: Pedone
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D008279:Magnetic Resonance Imaging; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C497604:SNCA protein, human; D051844:alpha-Synuclein
keywords: Alpha synuclein; CD analyses; NMR spectroscopy; Protein–membranes interaction