title: Expression of CD150 in tumors of the central nervous system: identification of a novel isoform.
abstract: CD150 (IPO3/SLAM) belongs to the SLAM family of receptors and serves as a major entry receptor for measles virus. CD150 is expressed on normal and malignant cells of the immune system. However, little is known about its expression outside the hematopoietic system, especially tumors of the central nervous system (CNS). Although CD150 was not found in different regions of normal brain tissues, our immunohistochemical study revealed its expression in 77.6% of human CNS tumors, including glioblastoma, anaplastic astrocytoma, diffuse astrocytoma, ependymoma, and others. CD150 was detected in the cytoplasm, but not on the cell surface of glioma cell lines, and it was colocalized with the endoplasmic reticulum and Golgi complex markers. In addition to the full length mRNA of the mCD150 splice isoform, in glioma cells we found a highly expressed novel CD150 transcript (nCD150), containing an 83 bp insert. The insert is derived from a previously unrecognized exon designated Cyt-new, which is located 510 bp downstream of the transmembrane region exon, and is a specific feature of primate SLAMF1. Both mCD150 and nCD150 cDNA variants did not contain any mutations and had the leader sequence. The nCD150 transcript was also detected in normal and malignant B lymphocytes, primary T cells, dendritic cells and macrophages; however, in glioma cells nCD150 was found to be the predominant CD150 isoform. Similarly to mCD150, cell surface expression of nCD150 allows wild type measles virus entry to the cell. Our data indicate that CD150 expression in CNS tumors can be considered a new diagnostic marker and potential target for novel therapeutic approaches.
authors.affiliation: Laboratory of signal transduction pathways, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine; CIRI, International Center for Infectiology Research, IbIV team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; CNRS, UMR5308, Lyon, France; Université Lyon 1, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France.
authors.forename: Olga
authors.initials: O
authors.lastname: Romanets-Korbut
authors.affiliation: Laboratory of immunogenetics, Institute of Molecular and Cellular Biology of SB RAS, Novosibirsk, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Najakshin
authors.affiliation: Laboratory of signal transduction pathways, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine.
authors.forename: Mariya
authors.initials: M
authors.lastname: Yurchenko
authors.affiliation: CNRS, UMR5308, Lyon, France.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Malysheva
authors.affiliation: Laboratory of signal transduction pathways, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine.
authors.forename: Larysa
authors.initials: L
authors.lastname: Kovalevska
authors.affiliation: Laboratory of signal transduction pathways, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine.
authors.forename: Larysa M
authors.initials: LM
authors.lastname: Shlapatska
authors.affiliation: Neuropathomorphology Department, A.P. Romodanov Institute of Neurosurgery NAMS of Ukraine, Kyiv, Ukraine.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Zozulya
authors.affiliation: Laboratory of immunogenetics, Institute of Molecular and Cellular Biology of SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Taranin
authors.affiliation: CIRI, International Center for Infectiology Research, IbIV team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; CNRS, UMR5308, Lyon, France; Université Lyon 1, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France.
authors.forename: Branka
authors.initials: B
authors.lastname: Horvat
authors.affiliation: Laboratory of signal transduction pathways, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine.
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Sidorenko
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000378:metabolism / Q000473:pathology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008459:Measles virus / Q000502:physiology; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1
chemical_list: D015703:Antigens, CD; D020033:Protein Isoforms; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1