title: Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809.
abstract: Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species. A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus. This phage was also able to infect Pantoea agglomerans strains. The genome of phiEa2809 is a double-stranded linear DNA 162,160 bp in length, including 145 ORFs and one tRNA gene. The phiEa2809 genomic sequence is similar to the genomes of the Serratia plymutica phage MAM1, Shigella phage AG-3, Dickeya phage vB DsoM LIMEstone1 and Salmonella phage ViI and lacks similarity to described E. amylovora phage genomes. Based on virion morphology (an icosahedral head, long contractile tail) and genome structure, phiEa2809 was classified as a member of Myoviridae, ViI-like bacteriophages group. PhiEa2809 is the firstly characterized ViI-like bacteriophage able to lyse E. amylovora.
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Belarusian State University, 220050 Minsk, Belarus lagonenkoal@mail.ru.
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Lagonenko
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Belarusian State University, 220050 Minsk, Belarus.
authors.forename: Olga
authors.initials: O
authors.lastname: Sadovskaya
authors.affiliation: Institute of Microbiology, National Academy of Sciences, 220141 Minsk, Belarus.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Valentovich
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Belarusian State University, 220050 Minsk, Belarus.
authors.forename: Anatoly N
authors.initials: AN
authors.lastname: Evtushenkov
mesh_terms: D044084:Erwinia amylovora / Q000821:virology*; D016679:Genome, Viral; D017900:Myoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D016366:Open Reading Frames; D020636:Pantoea / Q000821:virology*; D010935:Plant Diseases / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014771:Virion
keywords: Erwinia amylovora; Myoviridae; bacteriophage