title: Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid.
abstract: Docosahexaenoic acid (DHA, C22:6n-3) plays a vital role in the enhancement of human health, particularly for cognitive, neurological, and visual functions. Marine microalgae, such as members of the genus Aurantiochytrium, are rich in DHA and represent a promising source of omega-3 fatty acids. In this study, levels of glucose, yeast extract, sodium glutamate and sea salt were optimized for enhanced lipid and DHA production by a Malaysian isolate of thraustochytrid, Aurantiochytrium sp. SW1, using response surface methodology (RSM). The optimized medium contained 60 g/L glucose, 2 g/L yeast extract, 24 g/L sodium glutamate and 6 g/L sea salt. This combination produced 17.8 g/L biomass containing 53.9% lipid (9.6 g/L) which contained 44.07% DHA (4.23 g/L). The optimized medium was used in a scale-up run, where a 5 L bench-top bioreactor was employed to verify the applicability of the medium at larger scale. This produced 24.46 g/L biomass containing 38.43% lipid (9.4 g/L), of which 47.87% was DHA (4.5 g/L). The total amount of DHA produced was 25% higher than that produced in the original medium prior to optimization. This result suggests that Aurantiochytrium sp. SW1 could be developed for industrial application as a commercial DHA-producing microorganism.
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia.
authors.forename: Vidyah
authors.initials: V
authors.lastname: Manikan
authors.affiliation: Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia.
authors.forename: Mohd Sahaid
authors.initials: MS
authors.lastname: Kalil
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia.
authors.forename: Aidil Abdul
authors.initials: AA
authors.lastname: Hamid
mesh_terms: D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D050356:Lipid Metabolism; D058086:Microalgae / Q000378:metabolism*; D008956:Models, Chemical; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004281:Docosahexaenoic Acids