title: Determination of acacetin in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of acacetin in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 2.0 min and the elution of acacetin was at 0.83 min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reaction monitoring (MRM) of the transitions at m/z 285.3→242.2 for acacetin and m/z 237.2→194.3 for carbamazepine (internal standard). The calibration curve was linear over the range of 1-1600 ng/mL with a lower limit of quantitation (LLOQ) of 1.0 ng/mL. Mean recovery of acacetin in plasma was in the range of 78.4-85.2%. Intra-day and inter-day precision were both <10.5%. This method was successfully applied in pharmacokinetic study after intravenous administration of 5.0mg/kg acacetin in rats.
authors.affiliation: Department of Anesthesiology, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, Zhejiang, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Fan
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, Zhejiang, China.
authors.forename: De-yuan
authors.initials: DY
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, Zhejiang, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shan
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Ren-ai
authors.initials: RA
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Shanghai No.9 People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. Electronic address: ausword@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.affiliation: The 2nd Affiliated Hospital & Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address: r_ge@yahoo.com.
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Ge
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D047309:Flavones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047309:Flavones; C023717:acacetin
keywords: Acacetin; Pharmacokinetics; Rat plasma; UPLC-MS/MS