title: [Calix[4]arenes C-136 and C-137 hyperpolarize myometrium mitochondria membranes].
abstract: Calixarenes--supramolecular compounds interacting with bioactive molecules and ions that causes the changes in biochemical and biophysical processes. The aim of this work was to study the effects of calix[4]arenes C-136, C-137 and C-138 on the level of polarization of rat myometrium mitochondria membrane. Structure of synthesized calix[4]arene molecules was confirmed by the methods of 1H NMR and infra-red spectroscopy. Calix[4]arenes C-136 and C-137 possess two chalcone amide moieties at the lower rim, while the calix[4]arene C-138--only one. In case of calix[4]arenes C-136 and C-137 take place, accordingly, absence or presence of phenolic hydroxyl groups at the lower rim on the calix[4]arene skeleton. It was shown that calix[4]arenes C-136, C-137 and C-138 form micelles in a water medium and in the dimethylformamide (DMF). The irradiation of micelles with argon laser on flow cytometer results in appearance of autofluorescence. In the water medium calix[4]arene micelles interact with positively charged potential-sensitive fluorescent probe TMRM, that can testify to the presence of negative charge in these structures. However calix[4]arene micelles in DMF solution do not interact with TMRM. Mitochondrial membrane potential was measured using fluorescent dyes MTG and TMRM with confocal microscopy and fluorescent dye TMRM with flow cytometry. Experiments were conducted on myometrium cells in culture and on suspension of digitonin-permeabilized uterus myocytes. It was shown that a fluorescent signal was stable during time of experiment. Calix[4]arenes C-136 and C-137 (10 μM) hyperpolarize mitochondria membranes. A maximal effect was 173%. At the same time calix[4]arene C-138 did not influence on mitochondria membrane potential. Connection comes into question between structural organization of investigated calix[4]arene molecules and their influence on polarization of mitochondria membrane.
authors.forename: L G
authors.initials: LG
authors.lastname: Babich
authors.forename: S G
authors.initials: SG
authors.lastname: Shlykov
authors.forename: V I
authors.initials: VI
authors.lastname: Boĭko
authors.forename: M A
authors.initials: MA
authors.lastname: Kliachina
authors.forename: S A
authors.initials: SA
authors.lastname: Kosterin
mesh_terms: D000818:Animals; D047250:Calixarenes / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007477:Ions; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008823:Micelles; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects*; D009215:Myometrium / Q000737:chemistry / Q000187:drug effects*; D019906:Nuclear Magnetic Resonance, Biomolecular; D051381:Rats; D014867:Water / Q000737:chemistry
chemical_list: D007477:Ions; D008823:Micelles; D014867:Water; D047250:Calixarenes