title: Relevance and therapeutic possibility of PTEN-long in renal cell carcinoma.
abstract: PTEN-Long is a translational variant of PTEN (Phosphatase and Tensin Homolog). Like PTEN, PTEN-Long is able to antagonize the PI3K-Akt pathway and inhibits tumor growth. In this study, we investigated the role PTEN-Long plays in the development and progression of clear cell renal cell carcinoma (ccRCC) and explored the therapeutic possibility using proteinaceous PTEN-Long to treat ccRCC. We found that the protein levels of PTEN-Long were drastically reduced in ccRCC, which was correlated with increased levels of phosphorylated Akt (pAkt). Gain of function experiments showed overexpression of PTEN-Long in the ccRCC cell line 786-0 suppressed PI3K-Akt signaling, inhibited cell proliferation, migration and invasion, and eventually induced cell death. When purified PTEN-Long was added into cultured 786-0 cells, it entered cells, blocked Akt activation, and induced apoptosis involving Caspase 3 cleavage. Furthermore, PTEN-Long inhibited proliferation of 786-0 cells in xenograft mouse model. Our results implicated that understanding the roles of PTEN-Long in renal cell carcinogenesis has therapeutic significance.
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Lin
authors.affiliation: Critical Care Medicine, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Jitao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Institute of Urology, Zhifu, Yantai, Shandong, 264000, People's Republic of China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi hospital, Ningbo, Zhejiang, 315040, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D020033:Protein Isoforms; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D059368:RNA Isoforms; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: D020033:Protein Isoforms; D059368:RNA Isoforms; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase