title: High-speed video analysis of acoustically oscillated guinea pig stapes.
abstract: OBJECTIVE
  We investigated the ossicular movement in the near-intact middle ear in response to acoustic stimulation using a high-speed video camera and video analysis software program.
  
  
  METHODS
  We have designed a good visual access to the middle ear of the guinea pig by opening the ventral wall of the otic capsule, without injuring the sound-conducting structures, from the external auditory canal to the oval window. The high-speed video camera could record analysable ossicular motion up to 4000 frames per second.
  
  
  RESULTS
  The stapes showed reciprocal movement in the same frequency as the stimulating tone, and with an amplitude proportional to the stimulating sound intensity. Injury to the tympanic membrane attenuated the stapedial motion, which was recovered to that of the control level by patch repair of the perforation.
  
  
  CONCLUSIONS
  Our experimental set-up was capable of evaluating the conductive hearing, regardless of the status of the animal's sensorineural hearing or even life. Such a video analysis may provide a powerful tool to investigate the physiology of the middle ear.
authors.affiliation: Department of Otorhinolaryngology,Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishizu
authors.affiliation: Department of Otorhinolaryngology,Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yasui
authors.affiliation: Department of Otorhinolaryngology,Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.affiliation: Department of Otorhinolaryngology,Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.affiliation: Department of Otorhinolaryngology,Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
authors.forename: S
authors.initials: S
authors.lastname: Komune
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006168:Guinea Pigs; D006309:Hearing / Q000502:physiology; D008297:Male; D018715:Microscopy, Video / Q000379:methods*; D009225:Myringoplasty; D009991:Oscillometry / Q000379:methods*; D012984:Software; D013199:Stapes / Q000502:physiology*; D014432:Tympanic Membrane / Q000293:injuries
keywords: Bone Conduction