title: Effects of dietary n-6:n-3 PUFA ratio on fatty acid composition, free amino acid profile and gene expression of transporters in finishing pigs.
abstract: Revealing the expression patterns of fatty acid and amino acid transporters as affected by dietary n-6:n-3 PUFA ratio would be useful for further clarifying the importance of the balance between n-6 and n-3 PUFA. A total of ninety-six finishing pigs were fed one of four diets with the ratio of 1:1, 2·5:1, 5:1 and 10:1. Pigs fed the dietary n-6:n-3 PUFA ratio of 5:1 had the highest (P< 0·05) daily weight gain, and those fed the dietary n-6:n-3 PUFA ratio of 1:1 had the largest loin muscle area (P< 0·01). The concentration of n-3 PUFA was raised as the ratio declined (P< 0·05) in the longissimus dorsi and subcutaneous adipose tissue. The contents of tryptophan, tasty amino acids and branched-chain amino acids in the longissimus dorsi were enhanced in pigs fed the dietary n-6:n-3 PUFA ratios of 1:1-5:1. The mRNA expression level of the fatty acid transporter fatty acid transport protein-1 (FATP-1) was declined (P< 0·05) in the longissimus dorsi of pigs fed the dietary n-6:n-3 PUFA ratios of 1:1-5:1, and increased (P< 0·05) in the subcutaneous adipose tissue of pigs fed the dietary n-6:n-3 PUFA ratios of 5:1 and 10:1. The expression profile of FATP-4 was similar to those of FATP-1 in the adipose tissue. The mRNA expression level of the amino acid transceptors LAT1 and SNAT2 was up-regulated (P< 0·05) in the longissimus dorsi of pigs fed the dietary n-6:n-3 PUFA ratios of 1:1 and 2·5:1. In conclusion, maintaining the dietary n-6:n-3 PUFA ratios of 1:1-5:1 would facilitate the absorption and utilisation of fatty acids and free amino acids, and result in improved muscle and adipose composition.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Yehui
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Meimei
authors.initials: M
authors.lastname: Geng
authors.affiliation: Animal Nutrition Department, College of Animal Science and Livestock Production, Federal University of Agriculture,PMB 2240Abeokuta,Ogun State,Nigeria.
authors.forename: Oso Abimbola
authors.initials: OA
authors.lastname: Oladele
authors.affiliation: Department of Animal Science, North Carolina State University,Raleigh,NC27695,USA.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha Hunan410125,People's Republic of China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D026924:Amino Acid Transport System A / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D003433:Crosses, Genetic; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000032:analysis; D002149:Energy Intake; D050612:Fatty Acid Transport Proteins / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D027283:Large Neutral Amino Acid-Transporter 1 / Q000235:genetics / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage / Q000378:metabolism; D008460:Meat / Q000032:analysis; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013024:Soybean Oil / Q000008:administration & dosage / Q000378:metabolism; D050151:Subcutaneous Fat / Q000254:growth & development / Q000378:metabolism*; D034421:Sus scrofa / Q000254:growth & development / Q000378:metabolism*; D015430:Weight Gain
chemical_list: D026924:Amino Acid Transport System A; D000596:Amino Acids; D004041:Dietary Fats; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D027283:Large Neutral Amino Acid-Transporter 1; D020033:Protein Isoforms; D013024:Soybean Oil; D008043:Linseed Oil
keywords: Finishing pigs