title: Activation of caspase-8 and caspase-9 are required for PC12 cells differentiation.
abstract: Nerve growth factor- (NGF) induced potent activation of caspase-8, and caspase-9 in PC12 cells in a time-dependent manner. Peptide inhibition of caspase activity led to a potent reduction in neurite outgrowth in NGF-treated cells. Although NGF treatment resulted in cell-cycle G0/G1 arrest as detected by FACS cell-cycle analysis, caspase inhibitors (caspase-8 and caspase-9 inhibitors) could block the G0/G1 arrest in PC12 cells. We demonstrated the unique caspase cascade, caspase-8, caspase-3, and caspase-9 to occur, in this order. In conclusion, present results confirm a unique role for the caspase-8 and caspase-9 mediated signal cascade in the differentiation of PC12 cells.
authors.affiliation: a Department of Medicinal Biochemistry, School of Pharmacy , Aichi-Gakuin University , Nagoya , Japan.
authors.forename: Makio
authors.initials: M
authors.lastname: Mogi
authors.forename: Ayami
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000818:Animals; D053181:Caspase 8 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D002454:Cell Differentiation; D004789:Enzyme Activation; D016716:PC12 Cells; D051381:Rats
chemical_list: D053181:Caspase 8; D053453:Caspase 9
keywords: caspase-8; caspase-9; nerve growth factor; neurite outgrowth; rat pheochromocytoma cells