title: A new bisphenol A derivative for estrogen receptor binding studies with surface plasmon resonance.
abstract: A new bisphenol A (BPA) derivative was synthesized and immobilized onto the surface of a CM5 sensor chip for a binding study with orphan estrogen-related receptor γ using the surface plasmon resonance technique. The kinetic parameters, including dissociation and association constants and dissociation and association rate constants, were investigated. Moreover, a competitive inhibition assay was conducted to understand the competition relationship between the free BPA molecules in solution and the immobilized BPA molecules on the sensor chip surface. The CM5 sensor chip immobilized with the BPA derivative can be regenerated by using a high-concentration running buffer for repeated use more than 150 times without any adverse effect on its performance in the binding studies. The results indicate that the system has potential for further development as a sensitive surface plasmon resonance-based biosensor for endocrine-disrupting chemicals.
authors.affiliation: Department of Science and Environmental Studies, Centre for Education in Environmental Sustainability, The Hong Kong Institute of Education, Tai Po, Hong Kong, China.
authors.forename: Wing-Leung
authors.initials: WL
authors.lastname: Wong
authors.affiliation: Department of Science and Environmental Studies, Centre for Education in Environmental Sustainability, The Hong Kong Institute of Education, Tai Po, Hong Kong, China.
authors.forename: Cheuk-Fai
authors.initials: CF
authors.lastname: Chow
mesh_terms: D001559:Benzhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D052244:Endocrine Disruptors / Q000737:chemistry / Q000378:metabolism*; D007103:Immobilization; D007700:Kinetics; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000737:chemistry / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D011960:Receptors, Estrogen; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Biosensor; Bisphenol A; CM5 sensor chip; Estrogen-related receptor; Surface plasmon resonance