title: Optimization and technological development strategies of an antimicrobial extract from Achyrocline alata assisted by statistical design.
abstract: Achyrocline alata, known as Jateí-ka-há, is traditionally used to treat several health problems, including inflammations and infections. This study aimed to optimize an active extract against Streptococcus mutans, the main bacteria that causes caries. The extract was developed using an accelerated solvent extraction and chemometric calculations. Factorial design and response surface methodologies were used to determine the most important variables, such as active compound selectivity. The standardized extraction recovered 99% of the four main compounds, gnaphaliin, helipyrone, obtusifolin and lepidissipyrone, which represent 44% of the extract. The optimized extract of A. alata has a MIC of 62.5 μg/mL against S. mutans and could be used in mouth care products.
authors.affiliation: Laboratório de Farmacognosia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Demarque
authors.affiliation: Laboratório de Microbiologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Sonia Maria F
authors.initials: SM
authors.lastname: Fitts
authors.affiliation: Laboratório de Farmacognosia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Boaretto
authors.affiliation: Faculdade de Odontologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Júlio César Leite
authors.initials: JC
authors.lastname: da Silva
authors.affiliation: Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Vieira
authors.affiliation: Laboratório de Farmacologia e Inflamação, Setor de Biofisiofarmacologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Vanessa N P
authors.initials: VN
authors.lastname: Franco
authors.affiliation: Laboratório de Microbiologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Caroline B
authors.initials: CB
authors.lastname: Teixeira
authors.affiliation: Laboratório de Farmacologia e Inflamação, Setor de Biofisiofarmacologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Mônica C
authors.initials: MC
authors.lastname: Toffoli-Kadri
authors.affiliation: Laboratório de Farmacognosia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Carollo
mesh_terms: D036921:Achyrocline / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006586:Hexanes / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000378:metabolism; D059625:Liquid-Liquid Extraction; D051379:Mice; D008826:Microbial Sensitivity Tests; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D012107:Research Design; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D006586:Hexanes; D010936:Plant Extracts; D009569:Nitric Oxide; D006861:Hydrogen Peroxide