title: New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis.
abstract: The aim of the study was to evaluate the usefulness of 3 chimeric Toxoplasma gondii antigens, P35-MAG1, MIC1-ROP1 and MAG1-ROP1, in the serodiagnosis of an acute toxoplasmosis in humans. Proteins were produced as fusion proteins containing His tags ends and then further purified by metal affinity chromatography. Their application for the diagnosis of recently acquired T. gondii infection was tested in IgG and IgM enzyme-linked immunosorbent assays (ELISAs). At 100%, 77.3%, and 86.4%, respectively, the reactivity of the IgG ELISA using P35-MAG1, MIC1-ROP1, and MAG1-ROP1 for sera from patients where acute toxoplasmosis was suspected was significantly higher than for the samples from people with a chronic infection, at 26.2%, 36.1%, and 32.8%, respectively. Moreover, P35-MAG1, MIC1-ROP1, and MAG1-ROP1 detected IgM antibodies with a reactivity at 81.8%, 72.7%, and 59.1%, respectively. The results presented in the article show that, particularly, P35-MAG1 may be useful in the preliminary detection of recent T. gondii infection.
authors.affiliation: Chemical Faculty, Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Gdańsk, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Drapała
authors.affiliation: Chemical Faculty, Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Gdańsk, Poland.
authors.forename: Lucyna
authors.initials: L
authors.lastname: Holec-Gąsior
authors.affiliation: Chemical Faculty, Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Gdańsk, Poland. Electronic address: kur@pg.gda.pl.
authors.forename: Józef
authors.initials: J
authors.lastname: Kur
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012698:Serologic Tests / Q000379:methods*; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins
keywords: Chimeric antigens; Fusion proteins; IgG ELISA; IgM ELISA; Toxoplasmosis