title: Nano copper oxide-incorporated mesoporous carbon composite as multimode adsorbent for selective isolation of hemoglobin.
abstract: Assembly of nano-objects with tunable size, morphology and function into integrated nanostructures is critical for the development of a novel nanosystem in adsorption, sensing and drug/gene delivery. We demonstrate herein the fabrication of ordered mesoporous carbon by assembling uniform and highly dispersed copper-oxide (CuxOy) nanoparticles into the mesopores via evaporation of solvent from the mixture of triblock copolymer, carbon source and metal nitrate hydrate. The ordered 2D hexagonal mesoporous carbon composite possesses a large surface area of 580.8 cm(2)/g, a uniform pore size of 5.4 nm, a large pore volume of 0.64 cm(3)/g and a high metal content of 3.32 wt %. The mesoporous composite exhibits excellent adsorption selectivity and high adsorption capacity to hemoglobin (Hb) under the synergistic effect of hydrophobic and metal-affinity interactions as well as size exclusion. This facilitates multimode adsorption of hemoglobin fitting Langmuir adsorption model and offers an adsorption capacity of 1666.7 mg g(-1) for hemoglobin. The mesoporous composite is used for the isolation of hemoglobin from human whole blood with high purity. It demonstrates the potential of the copper-oxide nanoparticle-embedded mesoporous carbon composite in selective isolation/removal of specific protein species from biological sample matrixes.
authors.affiliation: Research Center for Analytical Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li-Gang
authors.initials: LG
authors.lastname: Xing
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Chen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D002846:Chromatography, Affinity; D003300:Copper / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D058608:Nanopores / Q000648:ultrastructure; D016062:Porosity; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D002244:Carbon; D003300:Copper; C000520:cuprous oxide
keywords: copper-oxide nanoparticles; hemoglobin; mesoporous carbon; multimodal adsorption