title: A new polymorphic positions discovered in mitochondrial DNA hypervariable region HVIII from central and north-central of Iraq.
abstract: The aim of this research is to study the mitochondria non-coding region by using Sanger sequencing technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction) is utilized to extract DNA. A portion of a non-coding region encompassing positions 438 to 574 for HVIII amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column were then sequenced and detected by using the ABI 3730xL DNA analyzer. The new polymorphic positions 469 and 482 that are described in this study may in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence, most promising for identification variants.
authors.affiliation: a Centre of Environmental Research , Babylon University , Hilla City , Iraq and.
authors.forename: Muhanned Abdulhassan
authors.initials: MA
authors.lastname: Kareem
authors.affiliation: b Department of Molecular Biology , Babylon University , Hilla City , Iraq.
authors.forename: Ameer Ibrahim
authors.initials: AI
authors.lastname: Abdulzahra
authors.affiliation: b Department of Molecular Biology , Babylon University , Hilla City , Iraq.
authors.forename: Imad Hadi
authors.initials: IH
authors.lastname: Hameed
authors.affiliation: b Department of Molecular Biology , Babylon University , Hilla City , Iraq.
authors.forename: Mohammed Abdullah
authors.initials: MA
authors.lastname: Jebor
mesh_terms: D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007493:Iraq; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial
keywords: Forensic; HVIII; Iraq; frequency; polymorphism