title: The effect of Walterinnesia aegyptia venom proteins on TCA cycle activity and mitochondrial NAD(+)-redox state in cultured human fibroblasts.
abstract: Fibroblast cultures were used to study the effects of crude Walterinnesia aegyptia venom and its F1-F7 protein fractions on TCA cycle enzyme activities and mitochondrial NAD-redox state. Confluent cells were incubated with 10 μg of venom proteins for 4 hours at 37°C. The activities of all studied TCA enzymes and the non-TCA mitochondrial NADP(+)-dependent isocitrate dehydrogenase underwent significant reductions of similar magnitude (50-60% of control activity) upon incubation of cells with the crude venom and fractions F4, F5, and F7 and 60-70% for fractions F3 and F6. In addition, the crude and fractions F3-F7 venom proteins caused a drop in mitochondrial NAD(+) and NADP(+) levels equivalent to around 25% of control values. Whereas the crude and fractions F4, F5, and F7 venom proteins caused similar magnitude drops in NADH and NADPH (around 55% of control levels), fractions F3 and F6 caused a more drastic drop (60-70% of control levels) of both reduced coenzymes. Results indicate that the effects of venom proteins could be directed at the mitochondrial level and/or the rates of NAD(+) and NADP(+) biosynthesis.
authors.affiliation: Medical and Molecular Genetics Research Chair, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
authors.forename: Hazem K
authors.initials: HK
authors.lastname: Ghneim
authors.affiliation: Medical and Molecular Genetics Research Chair, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
authors.forename: Yazeed A
authors.initials: YA
authors.lastname: Al-Sheikh
authors.affiliation: Medical and Molecular Genetics Research Chair, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia ; The Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
authors.forename: Mourad A M
authors.initials: MA
authors.lastname: Aboul-Soud
mesh_terms: D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects*; D017815:Elapidae; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects*; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry; D012910:Snake Venoms / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D011506:Proteins; D012910:Snake Venoms; D009243:NAD