title: Caleosin from Chlorella vulgaris TISTR 8580 is salt-induced and heme-containing protein.
abstract: Physiological and functional properties of lipid droplet-associated proteins in algae remain scarce. We report here the caleosin gene from Chlorella vulgaris encodes a protein of 279 amino acid residues. Amino acid sequence alignment showed high similarity to the putative caleosins from fungi, but less to plant caleosins. When the C. vulgaris TISTR 8580 cells were treated with salt stress (0.3 M NaCl), the level of triacylglycerol increased significantly. The mRNA contents for caleosin in Chlorella cells significantly increased under salt stress condition. Caleosin gene was expressed in E. coli. Crude extract of E. coli cells exhibited the cumene hydroperoxide-dependent oxidation of aniline. Absorption spectroscopy showed a peak around 415 nm which was decreased upon addition of cumene hydroperoxide. Native polyacrylamide gel electrophoresis suggests caleosin existed as the oligomer. These data indicate that a fresh water C. vulgaris TISTR 8580 contains a salt-induced heme-protein caleosin.
authors.affiliation: a Graduate School of Environmental and Human Sciences , Meijo University , Nagoya , Japan.
authors.forename: Pairpilin
authors.initials: P
authors.lastname: Charuchinda
authors.forename: Rungaroon
authors.initials: R
authors.lastname: Waditee-Sirisattha
authors.forename: Hakuto
authors.initials: H
authors.lastname: Kageyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamada
authors.forename: Sophon
authors.initials: S
authors.lastname: Sirisattha
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Aparat
authors.initials: A
authors.lastname: Mahakhant
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Takabe
mesh_terms: D000595:Amino Acid Sequence; D000814:Aniline Compounds / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D048408:Chlorella vulgaris / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006418:Heme / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D014280:Triglycerides; C402836:caleosin protein, Sesamum indicum; D006418:Heme; D012965:Sodium Chloride; C023650:aniline
keywords: Chlorella vulgaris; caleosin; microalga; oil body protein; salt stress