title: Different protein of Echinococcus granulosus stimulates dendritic induced immune response.
abstract: Cystic echinococcosis is a chronic infectious disease that results from a host/parasite interaction. Vaccination with ferritin derived from Echinococcus granulosus is a potential preventative treatment. To understand whether ferritin is capable of inducing a host immune response, we investigated the response of dendritic cells (DCs) to both recombinant ferritin protein and the hydatid fluid (HF) of E. granulosus. We evaluated the immunomodulatory potential of these antigens by performing, immunocytochemistry, electron microscopy and in vivo imaging of monocyte-derived murine DCs. During antigen stimulation of DCs, ferritin cause DCs maturation and induced higher levels of surface marker expression and activated T-cell proliferation and migration. On contrary, HF failed to induce surface marker expression and to stimulate T-cell proliferation. In response to HF, DCs produced interleukin-6 (IL-6), but no IL-12 and IL-10. DCs stimulated with ferritin produced high levels of cytokines. Overall, HF appears to induce host immunosuppression in order to ensure parasite survival via inhibits DC maturation and promotes Th2-dependent secretion of cytokines. Although ferritin also promoted DC maturation and cytokine release, it also activates CD4+T-cell proliferation, but regard of the mechanism of the Eg.ferritin induce host to eradicate E. granulosus were not clear.
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution,School of Life Sciences,Lanzhou University,222 South Tianshui Road,Lanzhou,Gansu 730000,China.
authors.forename: Yana
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Echinococcosis Laboratory,Ningxia Medical University,Yinchun 750004,China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Laboratory Medicine,Ningxia Medical University,Yinchuan 750004,China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Lv
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution,School of Life Sciences,Lanzhou University,222 South Tianshui Road,Lanzhou,Gansu 730000,China.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004443:Echinococcosis / Q000469:parasitology; D048209:Echinococcus granulosus / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D005293:Ferritins / Q000096:biosynthesis / Q000494:pharmacology*; D015870:Gene Expression; D015801:Helminth Proteins / Q000494:pharmacology*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D061251:Primary Cell Culture; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D045424:Complex Mixtures; D015801:Helminth Proteins; C556062:IL10 protein, mouse; D015850:Interleukin-6; D011994:Recombinant Proteins; D016753:Interleukin-10; D018664:Interleukin-12; D005293:Ferritins
keywords: Echinococcus granulosus; dendritic cell; hydatid fluid; immune response; recombinant ferritin