title: Novel polystyrene/antibody nanoparticle-coated capillary for immunoaffinity in-tube solid-phase microextraction.
abstract: Antibody-coated polystyrene (PS) nanoparticles (denoted as PS/IgG) were prepared and chemically immobilized for the first time onto a capillary inner wall for immunoaffinity in-tube solid-phase microextraction (SPME) of β2-microglobin (β2MG) and cystatin C (Cys-C). Scanning electron microscopy images of the prepared capillary showed that the nanoparticles were evenly coated onto the capillary inner surface, resulting in the undulating surface of the capillary inner wall. The extraction capacity of the nanoparticle-coated capillary was nearly five times higher than that of a monolayer antibody-immobilized capillary. The in-tube SPME recovery of β2MG (or Cys-C) by the nanoparticle-functionalized capillary was more than 97.8%, whereas that by the monolayer antibody-immobilized tube was 30.5%. In addition, the method quantitation limit obtained by using the nanoparticle-coated capillary was ten times lower than that by the monolayer capillary. Therefore, the capillary coated by PS/IgG nanoparticles is more suitable for immunoaffinity in-tube SPME compared with the commonly used monolayer antibody-immobilized capillary.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Xu
authors.forename: Shuai
authors.initials: S
authors.lastname: Cheng
authors.forename: Xianhua
authors.initials: X
authors.lastname: Wang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry; D055316:Cystatin C / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011786:Quality Control; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods*; D001613:beta 2-Microglobulin / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D055316:Cystatin C; D011137:Polystyrenes; D001613:beta 2-Microglobulin