title: Association of Hemostatic Gene Polymorphisms With Early-Onset Ischemic Heart Disease in Egyptian Patients.
abstract: The association between hereditary thrombophilia and venous thrombosis is well established but controversial data exist with respect to arterial thrombosis. We performed a pilot study on 31 patients with acute myocardial infarction (AMI), 21 patients with unstable angina (UA), and 20 healthy volunteers to investigate the role of various hemostatic gene polymorphisms in young Egyptian patients, who survived their first ischemic heart disease (IHD). Thrombophilic gene polymorphisms were tested using multiplex polymerase chain reaction and reverse-hybridization technique. We showed an increased risk of AMI with factor V (FV) Leiden and prothrombin G20210A heterozygosity. The increased risks of UA was associated with GA and A allele of fibrinogen β-455G→A polymorphism. Conversely, factor XIII (FXIII) Val34Leu GT and T allele were protective in the UA group. Nevertheless, the prevalence of FV H1299R, plasminogen activator inhibitor 1 4G/5G, glycoprotein IIIa C1565T, 5,10-methylenetetrahydrofolate reductase C677T, and A1298C mutations did not differ between patients with IHD and controls. The data have clinical implications regarding screening and thromboprophylaxis in high-risk individuals younger than 40 years.
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Wael
authors.initials: W
authors.lastname: Alkhiary
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Hanan
authors.initials: H
authors.lastname: Azzam
authors.affiliation: Department of Cardiology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Mahmoud Mohammed Abdo
authors.initials: MM
authors.lastname: Yossof
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Salah
authors.initials: S
authors.lastname: Aref
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada School of Baccalaureate Nursing, St Lawrence College, Kingston, Ontario, Canada othman@queensu.ca.
authors.forename: Maha
authors.initials: M
authors.lastname: Othman
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Solafa
authors.initials: S
authors.lastname: El-Sharawy
mesh_terms: D000328:Adult; D017668:Age of Onset; D016022:Case-Control Studies; D004534:Egypt; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006487:Hemostasis / Q000235:genetics*; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
keywords: arterial thrombosis; hereditary thrombophilia; ischemic heart disease; myocardial infarction; unstable angina