title: Inhibitory effect of triterpenoids from Dillenia serrata (Dilleniaceae) on prostaglandin E2 production and quantitative HPLC analysis of its koetjapic acid and betulinic acid contents.
abstract: The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. juriyati@pharmacy.ukm.my.
authors.forename: Juriyati
authors.initials: J
authors.lastname: Jalil
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. cw.sabandar@yahoo.com.
authors.forename: Carla W
authors.initials: CW
authors.lastname: Sabandar
authors.affiliation: Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam 40450, Malaysia. noriz118@salam.uitm.edu.my.
authors.forename: Norizan
authors.initials: N
authors.lastname: Ahmat
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. jamia@pharmacy.ukm.my.
authors.forename: Jamia A
authors.initials: JA
authors.lastname: Jamal
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. ibj@pharmacy.ukm.my.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Jantan
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. shila.aladdin@gmail.com.
authors.forename: Nor-Ashila
authors.initials: NA
authors.lastname: Aladdin
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. kartini_mohd@yahoo.com.
authors.forename: Kartiniwati
authors.initials: K
authors.lastname: Muhammad
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. fhataheya_buang@yahoo.com.
authors.forename: Fhataheya
authors.initials: F
authors.lastname: Buang
authors.affiliation: Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. hazni@pharmacy.ukm.my.
authors.forename: Hazni Falina
authors.initials: HF
authors.lastname: Mohamad
authors.affiliation: Laboratory of Natural Products Chemistry, Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia. sahidin02@yahoo.com.
authors.forename: Idin
authors.initials: I
authors.lastname: Sahidin
mesh_terms: D045466:Dilleniaceae / Q000737:chemistry*; D015232:Dinoprostone / Q000096:biosynthesis*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D053978:Pentacyclic Triterpenes; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology; D000094062:Betulinic Acid
chemical_list: D008070:Lipopolysaccharides; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C573794:koetjapic acid; D015232:Dinoprostone; D000094062:Betulinic Acid