title: OsSDS is essential for DSB formation in rice meiosis.
abstract: SDS is a meiosis specific cyclin-like protein and required for DMC1 mediated double-strand break (DSB) repairing in Arabidopsis. Here, we found its rice homolog, OsSDS, is essential for meiotic DSB formation. The Ossds mutant is normal in vegetative growth but both male and female gametes are inviable. The Ossds meiocytes exhibit severe defects in homologous pairing and synapsis. No γH2AX immunosignals in Ossds meiocytes together with the suppression of chromosome fragmentation in Ossds-1 Osrad51c, both provide strong evidences that OsSDS is essential for meiotic DSB formation. Immunostaining investigations revealed that meiotic chromosome axes are normally formed but both SC installation and localization of recombination elements are failed in Ossds. We suspected that this cyclin protein has been differentiated pretty much between monocots and dicots on its function in meiosis.
authors.affiliation: Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University Kunming, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China ; School of Life Sciences, Huaiyin Normal University Huaian, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University Kunming, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors.forename: Zhukuan
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University Kunming, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
keywords: DSB formation; OsSDS; meiosis; rice