title: Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.
abstract: Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.
authors.affiliation: Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Amnon
authors.initials: A
authors.lastname: Bar-Shir
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liang
authors.forename: Kannie W Y
authors.initials: KW
authors.lastname: Chan
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
mesh_terms: D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000145:classification; D057809:HEK293 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009154:Mutation; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins