title: Annexin A2 is required for the early steps of cytokinesis.
abstract: Cytokinesis requires the formation of an actomyosin contractile ring between the two sets of sister chromatids. Annexin A2 is a calcium- and phospholipid-binding protein implicated in cortical actin remodeling. We report that annexin A2 accumulates at the equatorial cortex at the onset of cytokinesis and depletion of annexin A2 results in cytokinetic failure, due to a defective cleavage furrow assembly. In the absence of annexin A2, the small GTPase RhoA-which regulates cortical cytoskeletal rearrangement-fails to form a compact ring at the equatorial plane. Furthermore, annexin A2 is required for cortical localization of the RhoGEF Ect2 and to maintain the association between the equatorial cortex and the central spindle. Our results demonstrate that annexin A2 is necessary in the early phase of cytokinesis. We propose that annexin A2 participates in central spindle-equatorial plasma membrane communication.
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France christelle.benaud@univ-rennes1.fr.
authors.forename: Christelle
authors.initials: C
authors.lastname: Benaud
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Le Dez
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Mironov
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: Federico
authors.initials: F
authors.lastname: Galli
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: David
authors.initials: D
authors.lastname: Reboutier
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290 Equipe Labellisée Ligue 2014, Rennes, France Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Prigent
mesh_terms: D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D045744:Cell Line, Tumor; D002842:Chromatids / Q000378:metabolism / Q000648:ultrastructure; D048749:Cytokinesis / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D017354:Point Mutation; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D009418:S100 Proteins; D020742:rhoA GTP-Binding Protein; D000097573:Red Fluorescent Protein; D000099248:S100 Calcium Binding Protein A10; C472822:ANXA2 protein, human; C489850:ECT2 protein, human; C066737:RHOA protein, human
keywords: RhoA; annexin A2; cytokinesis; mitosis