title: Sodium butyrate-induced histone hyperacetylation up-regulating WT1 expression in porcine kidney fibroblasts.
abstract: OBJECTIVE
  Wilms' tumor 1 gene (WT1) is essential for the development of kidney and histone acetylation and is involved in its expression regulation in mice. However, whether WT1 expression is associated with histone acetylation in porcine kidney cells is unclear. Here, the effect of histone deacetylase inhibitor sodium butyrate (NaBu)-induced hyperacetylation on WT1 expression in porcine kidney fibroblasts (PKF) was examined.
  
  
  RESULTS
  Treatments of NaBu (1, 3, 6 mM) for 24 h increased PKF viability, and 24, 48 h-treatments of 1 mM NaBu enhanced PKF proliferation. WT1 mRNA levels were significantly elevated in NaBu-treated (1, 3 mM for 24, 48 h, respectively) PKF samples. Consistently, strengthened expression of WT1 protein and histone acetylation level were detected in NaBu-treated PKF cells.
  
  
  CONCLUSIONS
  Together, NaBu-induced hyperacetylation up-regulates WT1 expression in PKF, suggesting the involvement of histone acetylation in the transcriptional modulation of WT1 in porcine kidney cells.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, 5333 Xi-An Road, Changchun, 130062, People's Republic of China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Tan
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Peipei
authors.initials: P
authors.lastname: An
authors.forename: Aibing
authors.initials: A
authors.lastname: Wang
authors.forename: Meiran
authors.initials: M
authors.lastname: Chu
authors.forename: Liuxin
authors.initials: L
authors.lastname: Shi
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhang
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000107:Acetylation; D000818:Animals; D020148:Butyric Acid / Q000378:metabolism*; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006657:Histones / Q000378:metabolism*; D013552:Swine; D015854:Up-Regulation; D025721:WT1 Proteins / Q000096:biosynthesis*
chemical_list: D004791:Enzyme Inhibitors; D006657:Histones; D025721:WT1 Proteins; D020148:Butyric Acid