title: Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein.
abstract: In clinic, it calls for effective and simple materials to repair etched dentin. Bioinspired by the natural mineralization process guided by noncollagenous proteins (NCPs), in this work, we synthesized the fourth generation phosphate-terminated polyamidoamine dendrimer (G4-PO3H2) by one-step modification. We used FT-IR and 1H NMR to characterize the structure of G4-PO3H2, and MTT assay to prove its biocompatibility. It was applied as the analog of dentin phosphophoryn (DPP: a type of NCPs) to repair dentin, due to its similar dimensional scale, topological architecture and peripheral functionalities to that of DPP. By the characterization of SEM and XRD, the effective regeneration of human dentin induced by G4-PO3H2 is characterized and illustrated both in vitro (artificial saliva) and in vivo (oral cavity of rats). It is noted that the thickness of the regenerated mineral layers are more than 10 μm both in vitro and in vivo. The design strategy of G4-PO3H2 may be valuable for researchers in the fields of material science, stomatology and medicine to prepare various promising restorative nano-materials for biomineralized hard tissues such as bone and teeth.
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Kunneng
authors.initials: K
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address: jiyao_li@aliyun.com.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address: helibang@163.com.
authors.forename: Libang
authors.initials: L
authors.lastname: He
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Shuangjuan
authors.initials: S
authors.lastname: Peng
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Xingyu
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4).
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ding
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China(4); State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. Electronic address: jianshu_li@scu.edu.cn.
authors.forename: Jianshu
authors.initials: J
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000138:chemical synthesis* / Q000494:pharmacology; D003764:Dental Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D003804:Dentin / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D008963:Molar / Q000737:chemistry / Q000601:surgery; D010750:Phosphoproteins / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000737:chemistry; D014081:Tooth Extraction; D000099311:Dentin Sialophosphoprotein
chemical_list: D050091:Dendrimers; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C104700:PAMAM Starburst
keywords: Dendrimer; Human dentin; Hydroxyapatite; Protein analogs; Restorative material