title: [Glutathione S-transferase of alpha class from pike liver].
abstract: In this study, glutathione S-transferase (GST) was isolated from the liver of pike Esox lucius, which was homogenous according to SDS-PAGE and isoelectrofocusing. It is a homodimer with subunits mass 25235.36 Da (according to HPLC-MS/MS) and pI about 6.4. Substrate specificity, thermostability, some kinetic characteristics and optimum pH were determined. The enzyme was identified as Alpha class GST.
authors.forename: E V
authors.initials: EV
authors.lastname: Borvinskaia
authors.forename: L P
authors.initials: LP
authors.lastname: Smirnov
authors.forename: N N
authors.initials: NN
authors.lastname: Nemova
mesh_terms: D000818:Animals; D002384:Catalysis; D002467:Cell Nucleus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D017859:Esocidae; D005982:Glutathione Transferase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha