title: [Display cellulolytic enzymes on Saccharomyces cerevisiae cell surface by using Flo1p as an anchor protein for cellulosic ethanol production].
abstract: In this study, we constructed a yeast consortium surface-display expression system by using Flo1 as an anchor protein. Endoglucanase II (EGII) and cellobiohydrolase II (CBHII) from Trichoderma reesei, and β3-glucosidase 1 (BGLI) from Aspergillus aculeatus were immobilized on Saccharomyces cerevisiae Y5. We constructed the cellulose-displaying expression yeast consortium (Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. The displayed cellulolytic enzymes was stabile during the 96-h fermentation. The yeast consortium produced 0.77 g/L ethanol from 10 g/L phosphoric acid swollen cellulose (PASC) within 96 h. The yield (in grams of ethanol produced per gram of carbohydrate consumed) was 0.35 g/g, which correspond to 68.6% of the theoretical yield.
authors.forename: Chunling
authors.initials: C
authors.lastname: Mo
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Yang
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Xiushan
authors.initials: X
authors.lastname: Yang
authors.forename: Shen
authors.initials: S
authors.lastname: Tian
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D002480:Cellulase / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics; D004800:Enzymes, Immobilized / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005959:Glucosidases / Q000235:genetics; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D002482:Cellulose; D005959:Glucosidases; C056373:endoglucanase 2; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase