title: Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways.
abstract: We present the results of a native fluorescence spectroscopy study of blood plasma of rats with experimental diabetes. It was shown that the fluorescence emission band shape at 320 nm excitation is the most indicative of hyperglycemia in the blood plasma samples. We provide the interpretation of this fact based on the changes in reduced nicotinamide adenine dinucleotide phosphate concentration due to glucose-related metabolic pathways and protein fluorescent cross-linking formation following nonenzymatic glycation.
authors.affiliation: M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, Russia.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Shirshin
authors.affiliation: Institute of Laser Physics SB RAS, pr. Lavrentyeva, 13/3, Novosibirsk 630090, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Cherkasova
authors.affiliation: M.V. Lomonosov Moscow State University, International Laser Center, Leninskie gory 1/62, Moscow 119991, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tikhonova
authors.affiliation: M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Berlovskaya
authors.affiliation: M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, RussiacM.V. Lomonosov Moscow State University, International Laser Center, Leninskie gory 1/62, Moscow 119991, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Priezzhev
authors.affiliation: M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, Russia.
authors.forename: Victor
authors.initials: V
authors.lastname: Fadeev
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000737:chemistry; D001835:Body Weight; D003432:Cross-Linking Reagents / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D005453:Fluorescence; D005786:Gene Expression Regulation; D005947:Glucose / Q000737:chemistry*; D006031:Glycosylation; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy*; D008297:Male; D053858:Metabolic Networks and Pathways; D009243:NAD / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*
chemical_list: D001786:Blood Glucose; D003432:Cross-Linking Reagents; D009243:NAD; D009249:NADP; D005947:Glucose