title: Amelioration of ischemia-reperfusion-induced muscle injury by the recombinant human MG53 protein.
abstract: BACKGROUND
  Ischemia-reperfusion injury (I-R) in skeletal muscle requires timely treatment.
  
  
  METHODS
  Rodent models of I-R injury were used to test the efficacy of recombinant human MG53 (rhMG53) protein for protecting skeletal muscle.
  
  
  RESULTS
  In a mouse I-R injury model, we found that mg53,-/- mice are more susceptible to I-R injury. rhMG53 applied intravenously to the wild-type mice protected I-R injured muscle, as demonstrated by reduced CK release and Evans blue staining. Histochemical studies confirmed beneficial effects of rhMG53. Of interest, rhMG53 did not protect against I-R injury in rat skeletal muscle. This was likely due to the fact that the plasma level of endogenous MG53 protein is high in rats.
  
  
  CONCLUSIONS
  Our data suggest that rhMG53 may be a potential therapy for protection against muscle trauma. A mouse model appears to be a better choice than a rat model for evaluating potential treatments for protecting skeletal muscle.
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, P.R. China.
authors.forename: Jincai
authors.initials: J
authors.lastname: Hou
authors.affiliation: US Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, 78234, USA.
authors.forename: Janet L
authors.initials: JL
authors.lastname: Roe
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Park
authors.affiliation: Division of Protein Therapeutics, TRIM-edicine, Inc., Columbus, Ohio, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
authors.affiliation: The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, P.R. China.
authors.forename: Yongqiu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, P.R. China.
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, P.R. China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.|Division of Protein Therapeutics, TRIM-edicine, Inc., Columbus, Ohio, USA.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Ma
authors.affiliation: US Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, 78234, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walters
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D000070584:Tripartite Motif Proteins
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; C558399:TRIM72 protein, human; D000070584:Tripartite Motif Proteins
keywords: TRIM72; creatine kinase; edema; muscle injury; tourniquet