title: Association of TNF-α-(308(GG)), IL-10(-819(TT)), IL-10(-1082(GG)) and IL-1R1(+1970(CC)) genotypes with the susceptibility and progression of leprosy in North Indian population.
abstract: Leprosy is an infectious disease caused by M. leprae. We analyzed 48 cytokine polymorphisms in 13 (pro as well as anti-inflammatory) cytokine genes using PCR-SSP assay in 102 leprosy patients and 120 healthy controls with intent to find out a link between cytokine polymorphisms and disease susceptibility. TNF-α (-308) GG, IL-10 (-819) TT, IL-10 (-1082) GG and IL1R (+1970) CC genotypes are found to be predominant (p=0.01, p=0.02, p=0.0001 and p=0.001, respectively) in both tuberculoid as well as lepromatous leprosy patients. This observation suggests these genotypes as play the central role(s) in the progression of disease. CBA assay demonstrates the varied serum concentration of these cytokines with respect to their genotypes. The above genotypes appeared as high producer genotypes in our study. Even in presence of high produce genotypes, TNF-α level are found to be affected/masked by the presence of IL-10 in leprosy patients. Expressional masking of TNF-α is associated with the expression of IL-10 in these patients. This is one the negative impact of SNP-SNP interaction in leprosy patients. Therefore, we can conclude that cytokine gene polymorphisms determine the predisposition to the leprosy progression.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Tarique
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
authors.forename: Raza Ali
authors.initials: RA
authors.lastname: Naqvi
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Santosh
authors.affiliation: Department of Biostatistics, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
authors.forename: Vineet Kumar
authors.initials: VK
authors.lastname: Kamal
authors.affiliation: Department of Dermatovenerology, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
authors.forename: Neena
authors.initials: N
authors.lastname: Khanna
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India. Electronic address: dnrao31151@gmail.com.
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018450:Disease Progression; D004587:Electrophoresis, Agar Gel; D005260:Female; D005784:Gene Amplification; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007918:Leprosy / Q000097:blood / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053573:Receptors, Interleukin-1 Type I / Q000097:blood / Q000235:genetics*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; C508590:IL1R1 protein, human; D053573:Receptors, Interleukin-1 Type I; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: Cytokine polymorphism; Leprosy; SNP–SNP interaction; Serum cytokines