title: A Potent Virus-Specific Antibody-Secreting Cell Response to Acute Enterovirus 71 Infection in Children.
abstract: BACKGROUND
  Enterovirus 71 (EV71) remains a leading pathogen for acute infectious diseases in children, especially in Asia. The cellular basis for establishing a virus-specific antibody response to acute EV71 infections is unclear in children.
  
  
  METHODS
  We studied the magnitude of virus-specific antibody-secreting B cells (ASCs) and its relationship with serological response, clinical parameters, and virological parameters among children with laboratory-confirmed EV71 infection.
  
  
  RESULTS
  A potent EV71 genogroup B- and virus-specific ASC response was detected in the first week of illness among genotype B5 EV71-infected children. The cross-reactive EV71-specific ASC response to genogroup C viral antigens composed about 10% of the response. The EV71-specific ASC response in children aged ≥3 years produced immunoglobulin G predominantly, but immunoglobulin M was predominant in younger children. Proliferation marker was expressed by the majority of circulating ASCs in the acute phase of EV71 infection. Virus-specific ASC responses significantly correlated with throat viral load, fever duration, and serological genogroup-specific neutralization titer.
  
  
  CONCLUSIONS
  The presence of a virus-specific ASC response serves an early cellular marker of an EV71-specific antibody response. Further detailed study of EV71-specific ASCs at the monoclonal level is crucial to delineate the specificity and function of antibody immunity in children.
authors.affiliation: Department of Pediatrics, Chang Gung Children's Hospital Molecular Infectious Disease Research Centre.
authors.forename: Kuan-Ying Arthur
authors.initials: KY
authors.lastname: Huang
authors.affiliation: Department of Pediatrics, Chang Gung Children's Hospital.
authors.forename: Jainn-Jim
authors.initials: JJ
authors.lastname: Lin
authors.affiliation: Department of Pediatrics, Chang Gung Children's Hospital Molecular Infectious Disease Research Centre.
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Chiu
authors.affiliation: Department of Laboratory Medicine, Chang Gung Memorial Hospital.
authors.forename: Shuan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Chang Gung Memorial Hospital.
authors.forename: Kuo-Chien
authors.initials: KC
authors.lastname: Tsao
authors.affiliation: Department of Pediatrics, Chang Gung Children's Hospital College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Yhu-Chering
authors.initials: YC
authors.lastname: Huang
authors.affiliation: College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Tzou-Yien
authors.initials: TY
authors.lastname: Lin
mesh_terms: D000293:Adolescent; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D000921:Antibody-Producing Cells / Q000276:immunology*; D001208:Asia; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D029821:Enterovirus A, Human / Q000276:immunology* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D005260:Female; D005334:Fever; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D010614:Pharynx / Q000821:virology; D011446:Prospective Studies; D019562:Viral Load
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: antibody-secreting B cells; children; enterovirus 71