title: The cadmium-tolerant pea (Pisum sativum L.) mutant SGECdt is more sensitive to mercury: assessing plant water relations.
abstract: Heavy metals have multiple effects on plant growth and physiology, including perturbation of plant water status. These effects were assessed by exposing the unique Cd-tolerant and Cd-accumulating pea (Pisum sativum L.) mutant SGECd(t) and its wild-type (WT) line SGE to either cadmium (1, 4 μM CdCl2) or mercury (0.5, 1, 2 μM HgCl2) in hydroponic culture for 12 days. When exposed to Cd, SGECd(t) accumulated more Cd in roots, xylem sap, and shoot, and had considerably more biomass than WT plants. WT plants lost circa 0.2 MPa turgor when grown in 4 μM CdCl2, despite massive decreases in whole-plant transpiration rate and stomatal conductance. In contrast, root Hg accumulation was similar in both genotypes, but WT plants accumulated more Hg in leaves and had a higher stomatal conductance, and root and shoot biomass compared with SGECd(t). Shoot excision resulted in greater root-pressure induced xylem exudation of SGECd(t) in the absence of Cd or Hg and following Cd exposure, whereas the opposite response or no genotypic differences occurred following Hg exposure. Exposing plants that had not been treated with metal to 50 μM CdCl2 for 1h increased root xylem exudation of WT, whereas 50 μM HgCl2 inhibited and eliminated genotypic differences in root xylem exudation, suggesting differences between WT and SGECd(t) plants in aquaporin function. Thus, root water transport might be involved in mechanisms of increased tolerance and accumulation of Cd in the SGECd(t) mutant. However, the lack of cross-tolerance to Cd and Hg stress in the mutant indicates metal-specific mechanisms related to plant adaptation.
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 St-Petersburg, Russian-Federation belimov@rambler.ru.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Belimov
authors.affiliation: Lancaster Environment Centre, Lancaster University, LA1 4YQ Lancaster, UK.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Dodd
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 St-Petersburg, Russian-Federation.
authors.forename: Vera I
authors.initials: VI
authors.lastname: Safronova
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 St-Petersburg, Russian-Federation.
authors.forename: Nikita V
authors.initials: NV
authors.lastname: Malkov
authors.affiliation: Lancaster Environment Centre, Lancaster University, LA1 4YQ Lancaster, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Davies
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 St-Petersburg, Russian-Federation.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Tikhonovich
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D018533:Biomass; D002104:Cadmium / Q000633:toxicity*; D005838:Genotype; D008628:Mercury / Q000633:toxicity*; D009154:Mutation / Q000235:genetics*; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D054046:Plant Stomata / Q000187:drug effects; D018526:Plant Transpiration / Q000187:drug effects; D013997:Time Factors; D014867:Water / Q000378:metabolism*; D052584:Xylem / Q000187:drug effects / Q000378:metabolism
chemical_list: D002104:Cadmium; D014867:Water; D008628:Mercury
keywords: Aquaporin; cadmium; drought; mercury; pea; root sap flow; water deficit.