title: [Synthesis of recombinant gamma interferons resistant to proteolysis in the yeast Pichia pastoris].
abstract: Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia Pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity. The recombinant strains provide a useful tool for the purification of bovine and chicken gamma interferons for their use in veterinary applications.
authors.forename: M A
authors.initials: MA
authors.lastname: Tsygankov
authors.forename: A E
authors.initials: AE
authors.lastname: Zobnina
authors.forename: M V
authors.initials: MV
authors.lastname: Padkina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002645:Chickens / Q000276:immunology; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000821:virology; D015870:Gene Expression; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D018116:Vesiculovirus / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma