title: Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.
abstract: Activation of AMPA receptors assembled with the GluA1 subunit can promote dendrite growth in a manner that depends on its direct binding partner, SAP97. SAP97 is a modular scaffolding protein that has at least seven recognizable protein-protein interaction domains. Several complementary approaches were employed to show that the dendrite branching promoting action of full length SAP97 depends on ligand(s) that bind to the PDZ3 domain. Ligand(s) to PDZ1, PDZ2 and I3 domains also contribute to dendrite growth. The ability of PDZ3 ligand(s) to promote dendrite growth depends on localization at the plasma membrane along with GluA1 and SAP97. These results suggest that the assembly of a multi-protein complex at or near synapses is vital for the translation of AMPA-R activity into dendrite growth.
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States. Electronic address: ZHANGL@email.chop.edu.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States. Electronic address: HSUF@email.chop.edu.
authors.forename: F-C
authors.initials: FC
authors.lastname: Hsu
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States. Electronic address: PETROVIC@email.chop.edu.
authors.forename: J
authors.initials: J
authors.lastname: Mojsilovic-Petrovic
authors.affiliation: Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, United States. Electronic address: ajablo@mail.med.upenn.edu.
authors.forename: A M
authors.initials: AM
authors.lastname: Jablonski
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States. Electronic address: zhaijb@yahoo.com.
authors.forename: J
authors.initials: J
authors.lastname: Zhai
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, United States; Department of Neurology, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, United States. Electronic address: COULTERD@email.chop.edu.
authors.forename: D A
authors.initials: DA
authors.lastname: Coulter
authors.affiliation: Department of Pediatrics, Division of Neurology, Research Institute, Children's Hospital of Philadelphia, 3615 Civic Center Boulevard, Philadelphia, PA 19104, United States; Department of Neurology, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, United States. Electronic address: kalb@email.chop.edu.
authors.forename: R G
authors.initials: RG
authors.lastname: Kalb
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D055495:Neurogenesis; D054731:PDZ Domains; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D018091:Receptors, AMPA; C501119:Dlg1 protein, rat
keywords: GluA1; PDZ domain; Spinal cord neuron