title: Secondary structure analysis of swine pasivirus (family Picornaviridae) RNA reveals a type-IV IRES and a parechovirus-like 3' UTR organization.
abstract: The potential RNA structures of the 5' and 3' untranslated regions (UTRs) and cis-acting replication elements (CREs) of a novel pasivirus (PaV) genotype (family Picornaviridae) were analysed. PaV-A3 (KM259923) was identified in a faecal sample from a domestic pig in Hungary with posterior paraplegia of unknown etiology. Based on likely structural features of the 5' UTR, the pasiviruses were inferred to possess Hepacivirus/Pestivirus-like type-IV IRES. The pasivirus CRE was mapped to the 2B genome region, similar to Ljungan virus. The secondary RNA structure of the pasivirus 3' UTR was structurally similar to that of human parechoviruses. The genome, CRE, and 3' UTR of pasiviruses provide further evidence of the common origin of the members of the genera Parechovirus and Pasivirus, although their different 5' UTR IRES types suggest that a recombination event occurred during the divergence these viruses.
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, 7623, Pécs, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Boros
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Fenyvesi
authors.forename: Péter
authors.initials: P
authors.lastname: Pankovics
authors.forename: Hunor
authors.initials: H
authors.lastname: Biró
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.forename: Gábor
authors.initials: G
authors.lastname: Reuter
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D006814:Hungary; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010264:Paraplegia / Q000662:veterinary / Q000821:virology; D010849:Picornaviridae / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D059370:RNA Folding; D012367:RNA, Viral / Q000737:chemistry*; D012270:Ribosomes / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral