title: Superior cell penetration by a rigid and anisotropic synthetic protein.
abstract: Molecules with structural anisotropy and rigidity, such as asbestos, demonstrate high cell-penetrating activity but also high toxicity. Here we synthesize a biodegradable, rigid, and fibrous artificial protein, CCPC 140, as a potential vehicle for cellular delivery. CCPC 140 penetrated 100% of cells tested in vitro, even at a concentration of 3.1 nM-superior to previously reported cell-penetrating peptides. The effects of cell-strain-dependency and aspect ratio on the cell-penetrating activity of CCPC 140 were also investigated.
authors.affiliation: Graduate School of Environmental Symbiotic System Major and ‡Department of Innovative Systems Engineering, Nippon Institute of Technology , Miyashiro, Saitama 345-8501, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Nakayama
authors.forename: Kyoji
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Kuniharu
authors.initials: K
authors.lastname: Ijiro
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Osada
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016880:Anisotropy; D057846:Cell-Penetrating Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008297:Male; D055595:Mechanical Phenomena; D008969:Molecular Sequence Data; D011817:Rabbits; D014335:Tropomyosin / Q000737:chemistry
chemical_list: D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D014335:Tropomyosin