title: Mameliella phaeodactyli sp. nov., a member of the family Rhodobacteraceae isolated from the marine algae Phaeodactylum tricornutum.
abstract: A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53(T), was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53(T) was a member of the Roseobacter clade within the family Rhodobacteraceae , forming a distinct lineage with species of the genus Mameliella . The 16S rRNA gene sequence similarities between strain KD53(T) and other strains examined were all less than 97.0%. Strain KD53(T) was found to grow optimally at 28 °C, at pH 7.5-8.0 and in the presence of 3% (w/v) NaCl. The dominant fatty acids of strain KD53(T) were C18 : 1ω6c and/or C18 : 1ω7c, C18 : 0 and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53(T) represents a novel member of the genus Mameliella , then the name Mameliella phaeodactyli sp. nov. is proposed. The type strain is KD53(T) ( =MCCC 1K00273(T) =KCTC 42178(T)).
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Zhangran
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Lei
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Luxi
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Marine Environment and Key lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D017377:Diatoms / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2