title: KdmA, a histone H3 demethylase with bipartite function, differentially regulates primary and secondary metabolism in Aspergillus nidulans.
abstract: Aspergillus nidulans kdmA encodes a member of the KDM4 family of jumonji histone demethylase proteins, highly similar to metazoan orthologues both within functional domains and in domain architecture. This family of proteins exhibits demethylase activity towards lysines 9 and 36 of histone H3 and plays a prominent role in gene expression and chromosome structure in many species. Mass spectrometry mapping of A. nidulans histones revealed that around 3% of bulk histone H3 carried trimethylated H3K9 (H3K9me3) but more than 90% of histones carried either H3K36me2 or H3K36me3. KdmA functions as H3K36me3 demethylase and has roles in transcriptional regulation. Genetic manipulation of KdmA levels is tolerated without obvious effect in most conditions, but strong phenotypes are evident under various conditions of stress. Transcriptome analysis revealed that - in submerged early and late cultures - between 25% and 30% of the genome is under KdmA influence respectively. Transcriptional imbalance in the kdmA deletion mutant may contribute to the lethal phenotype observed upon exposure of mutant cells to low-density visible light on solid medium. Although KdmA acts as transcriptional co-repressor of primary metabolism genes, it is required for full expression of several genes involved in biosynthesis of secondary metabolites.
authors.affiliation: Fungal Genetics and Genomics Unit, Department of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Life Sciences, Campus Tulln, Tulln, 3430, Austria.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gacek-Matthews
authors.affiliation: Department of Genetics, University of Melbourne, Victoria, 3010, Australia.
authors.forename: Luke M
authors.initials: LM
authors.lastname: Noble
authors.affiliation: Department of Chemistry, BOKU-University of Natural Resources and Life Sciences, Campus Muthgasse, Vienna, A-1190, Austria.
authors.forename: Clemens
authors.initials: C
authors.lastname: Gruber
authors.affiliation: Health and Environment Department, AIT - Austrian Institute of Technology GmbH, Campus Tulln, Tulln, 3430, Austria.
authors.forename: Harald
authors.initials: H
authors.lastname: Berger
authors.affiliation: Center for Analytical Chemistry, Department IFA Tulln, BOKU-University of Natural Resources and Life Sciences, Campus Tulln, Tulln, 3430, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Sulyok
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Sevilla, 41012, Spain.
authors.forename: Ana T
authors.initials: AT
authors.lastname: Marcos
authors.affiliation: Fungal Genetics and Genomics Unit, Department of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Life Sciences, Campus Tulln, Tulln, 3430, Austria.|Health and Environment Department, AIT - Austrian Institute of Technology GmbH, Campus Tulln, Tulln, 3430, Austria.
authors.forename: Joseph
authors.initials: J
authors.lastname: Strauss
authors.affiliation: Department of Genetics, University of Melbourne, Victoria, 3010, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Andrianopoulos
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D056970:Co-Repressor Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008027:Light; D008239:Lysine / Q000378:metabolism; D013058:Mass Spectrometry; D008745:Methylation; D008958:Models, Molecular; D010641:Phenotype; D010802:Phylogeny; D064210:Secondary Metabolism; D017384:Sequence Deletion
chemical_list: D056970:Co-Repressor Proteins; D006657:Histones; D056466:Histone Demethylases; D008239:Lysine