title: The rhodopsin-arrestin-1 interaction in bicelles.
abstract: G-protein-coupled receptors (GPCRs) are essential mediators of information transfer in eukaryotic cells. Interactions between GPCRs and their binding partners modulate the signaling process. For example, the interaction between GPCR and cognate G protein initiates the signal, while the interaction with cognate arrestin terminates G-protein-mediated signaling. In visual signal transduction, arrestin-1 selectively binds to the phosphorylated light-activated GPCR rhodopsin to terminate rhodopsin signaling. Under physiological conditions, the rhodopsin-arrestin-1 interaction occurs in highly specialized disk membrane in which rhodopsin resides. This membrane is replaced with mimetics when working with purified proteins. While detergents are commonly used as membrane mimetics, most detergents denature arrestin-1, preventing biochemical studies of this interaction. In contrast, bicelles provide a suitable alternative medium. An advantage of bicelles is that they contain lipids, which have been shown to be necessary for normal rhodopsin-arrestin-1 interaction. Here we describe how to reconstitute rhodopsin into bicelles, and how bicelle properties affect the rhodopsin-arrestin-1 interaction.
authors.affiliation: Department of Pharmacology, Vanderbilt University Medical Center, 1211 Medical Center Drive, Nashville, TN, 37232-6600, USA.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Chen
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Vishnivetskiy
authors.forename: Tiandi
authors.initials: T
authors.lastname: Zhuang
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Cho
authors.forename: Tarjani M
authors.initials: TM
authors.lastname: Thaker
authors.forename: Charles R
authors.initials: CR
authors.lastname: Sanders
authors.forename: Vsevolod V
authors.initials: VV
authors.lastname: Gurevich
authors.forename: T M
authors.initials: TM
authors.lastname: Iverson
mesh_terms: D019393:Arrestin / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D010766:Phosphorylation; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D019393:Arrestin; D043562:Receptors, G-Protein-Coupled; D012243:Rhodopsin