title: An effective electrochemical biosensing platform for the detection of reduced glutathione.
abstract: A biosensor was developed using glutathione peroxidase (GSH-Px) as the enzyme. Firstly, platinum (Pt) nanoparticles were deposited onto a glassy carbon paste electrode (GCPE), and then GSH-Px was immobilized by means of gelatin that was then crosslinked with glutaraldehyde. The measurement was based on the electrochemical oxidation of GSH to its disulfide form in the presence of hydrogen peroxide. The linear range was found to be between 10 and 250 μM, with a correlation coefficient of R(2) = 0.9968. The R.S.D value for 25 μM GSH (n = 6) was calculated as 2.92%. Finally, the proposed biosensor was used to analyze GSH in a synthetically prepared plasma sample, and a promising recovery value was obtained.
authors.affiliation: a Department of Chemistry , Mugla Sitki Kocman University, Faculty of Science , Kötekli, Mugla , Turkiye.
authors.forename: Ülkü
authors.initials: Ü
authors.lastname: Anik
authors.affiliation: a Department of Chemistry , Mugla Sitki Kocman University, Faculty of Science , Kötekli, Mugla , Turkiye.|b Department of Chemistry , Ege University, Faculty of Science , Bornova, Izmir , Turkiye.
authors.forename: Meliha
authors.initials: M
authors.lastname: Çubukçu
authors.affiliation: b Department of Chemistry , Ege University, Faculty of Science , Bornova, Izmir , Turkiye.
authors.forename: F Nil
authors.initials: FN
authors.lastname: Ertaş
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D002244:Carbon; D006861:Hydrogen Peroxide; D005978:Glutathione
keywords: Pt nanoparticles; biosensor; glassy carbon paste electrode; glutathione; glutathione peroxidase