title: Effects of berberine on amelioration of hyperglycemia and oxidative stress in high glucose and high fat diet-induced diabetic hamsters in vivo.
abstract: This study investigated the effects of berberine on amelioration of hyperglycemia and hyperlipidemia and the mechanism involved in high glucose and high fat diet-induced diabetic hamsters. Golden hamsters fed with high glucose and high fat diet were medicated with metformin, simvastatin, and low or high dose of berberine (50 and 100 mg·kg(-1)) for 6 weeks. The results showed that the body weights were significantly lower in berberine-treated groups than control group. Histological analyses revealed that the treatment of berberine inhibited hepatic fat accumulation. Berberine significantly reduced plasma total cholesterol, triglyceride, free fatty acid, low density lipoprotein cholesterol, malondialdehyde, thiobarbituric acid-reactive substance, and 8-isoprostane level but significantly increased plasma superoxide dismutase activity. Glucose and insulin levels were significantly reduced in metformin and berberine-treated groups. Glucose tolerance tests documented that berberine-treated mice were more glucose tolerant. Berberine treatment increased expression of skeletal muscle glucose transporter 4 mRNA and significantly decreased liver low density lipoprotein receptor mRNA expression. The study suggested that berberine was effective in lowering blood glucose and lipids levels, reducing the body weight, and alleviating the oxidative stress in diabetic hamsters, which might be beneficial in reducing the cardiovascular risk factors in diabetes.
authors.affiliation: Department of Endocrinology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Endocrinology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Endocrinology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Endocrinology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D001599:Berberine / Q000008:administration & dosage*; D001786:Blood Glucose / Q000187:drug effects; D002318:Cardiovascular Diseases / Q000097:blood / Q000150:complications / Q000188:drug therapy; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D059305:Diet, High-Fat; D015870:Gene Expression / Q000187:drug effects; D005951:Glucose Tolerance Test; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008647:Mesocricetus; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D012333:RNA, Messenger; D001599:Berberine; D013311:Streptozocin