title: Regulation of DEAH/RHA helicases by G-patch proteins.
abstract: RNA helicases from the DEAH/RHA family are present in all the processes of RNA metabolism. The function of two helicases from this family, Prp2 and Prp43, is regulated by protein partners containing a G-patch domain. The G-patch is a glycine-rich domain discovered by sequence alignment, involved in protein-protein and protein-nucleic acid interaction. Although it has been shown to stimulate the helicase's enzymatic activities, the precise role of the G-patch domain remains unclear. The role of G-patch proteins in the regulation of Prp43 activity has been studied in the two biological processes in which it is involved: splicing and ribosome biogenesis. Depending on the pathway, the activity of Prp43 is modulated by different G-patch proteins. A particular feature of the structure of DEAH/RHA helicases revealed by the Prp43 structure is the OB-fold domain in C-terminal part. The OB-fold has been shown to be a platform responsible for the interaction with G-patch proteins and RNA. Though there is still no structural data on the G-patch domain, in the current model, the interaction between the helicase, the G-patch protein, and RNA leads to a cooperative binding of RNA and conformational changes of the helicase.
authors.affiliation: Laboratoire de Cristallographie et RMN Biologiques, UMR CNRS 8015, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris-Cité, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Robert-Paganin
authors.affiliation: Laboratoire de Cristallographie et RMN Biologiques, UMR CNRS 8015, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris-Cité, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Réty
authors.affiliation: Laboratoire de Cristallographie et RMN Biologiques, UMR CNRS 8015, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris-Cité, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
mesh_terms: D000595:Amino Acid Sequence; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D012313:RNA; D019204:GTP-Binding Proteins; D053487:DEAD-box RNA Helicases