title: A whole genome screening and RNA interference identify a juvenile hormone esterase-like gene of the diamondback moth, Plutella xylostella.
abstract: Juvenile hormone (JH) plays a crucial role in preventing precocious metamorphosis and stimulating reproduction. Thus, its hemolymph titer should be under a tight control. As a negative controller, juvenile hormone esterase (JHE) performs a rapid breakdown of residual JH in the hemolymph during last instar to induce a larval-to-pupal metamorphosis. A whole genome of the diamondback moth (DBM), Plutella xylostella, has been annotated and proposed 11 JHE candidates. Sequence analysis using conserved motifs commonly found in other JHEs proposed a putative JHE (Px004817). Px004817 (64.61 kDa, pI=5.28) exhibited a characteristic JHE expression pattern by showing high peak at the early last instar, at which JHE enzyme activity was also at a maximal level. RNA interference of Px004817 reduced JHE activity and interrupted pupal development with a significant increase of larval period. This study identifies Px004817 as a JHE-like gene of P. xylostella.
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, People's Republic of China; Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Marine Engineering, Kunsan National University, Kunsan, Republic of Korea.
authors.forename: Eunjin
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea. Electronic address: hosanna@andong.ac.kr.
authors.forename: Yonggyun
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D049750:Genome, Insect; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000196:embryology / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D034622:RNA Interference; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase
keywords: Genome; Juvenile hormone esterase; Metamorphosis; Plutella xylostella; RNA interference