title: Enhancement of Bacillus thuringiensis insecticidal activity by combining Cry1Ac and bi-functional toxin HWTX-XI from spider.
abstract: In order to assess the potency of bi-functional HWTX-XI toxin from spider Ornithoctonus huwena in improving the insecticidal activity of Bacillus thuringiensis, a fusion gene of cry1Ac and hwtx-XI was constructed and expressed in an acrystalliferous B. thuringiensis strain Cry(-)B. Western blot analysis and microscopic observation revealed that the recombinant strain could express 140-kDa Cry1Ac-HWTX-XI fusion protein and produce parasporal inclusions during sporulation. Bioassay using the larvae of Helicoverpa armigera and Spodoptera exigua showed that the Cry1Ac-HWTX-XI fusion was more toxic than the control Cry1Ac protoxin, as revealed by 95% lethal concentration. Our study indicated that the HWTX-XI from spider might be a candidate for enhancing the toxicity of B. thuringiensis products.
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China; Hunan Institute of Microbiology, Changsha 410009, PR China.
authors.forename: Zujiao
authors.initials: Z
authors.lastname: Fu
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: He
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yuan
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, PR China. Electronic address: xialq@hunnu.edu.cn.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001681:Biological Assay; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D009036:Moths / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013111:Spider Venoms / Q000494:pharmacology*; D013112:Spiders / Q000737:chemistry*; D018411:Spodoptera / Q000187:drug effects
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D011993:Recombinant Fusion Proteins; D013111:Spider Venoms; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Cry1Ac; Fusion protein; Insecticidal activity; Spider toxin