title: An ultrahigh-performance liquid chromatography method with electrospray ionization tandem mass spectrometry for simultaneous quantification of five phytohormones in medicinal plant Glycyrrhiza uralensis under abscisic acid stress.
abstract: An efficient simplified method was developed to determine multiple classes of phytohormones simultaneously in the medicinal plant Glycyrrhiza uralensis. Ultrahigh-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) with multiple reaction monitoring (MRM) in negative mode was used for quantification. The five studied phytohormones are gibberellic acid (GA3), abscisic acid (ABA), jasmonic acid (JA), indole-3-acetic acid, and salicylic acid (SA). Only 100 mg of fresh leaves was needed, with one purification step based on C18 solid-phase extraction. Cinnamic acid was chosen as the internal standard instead of isotope-labeled internal standards. Under the optimized conditions, the five phytohormones with internal standard were separated within 4 min, with good linearities and high sensitivity. The validated method was applied to monitor the spatial and temporal changes of the five phytohormones in G. uralensis under ABA stress. The levels of GA3, ABA, JA, and SA in leaves of G. uralensis were increased at different times and with different tendencies in the reported stress mode. These changes in phytohormone levels are discussed in the context of a possible feedback regulation mechanism. Understanding this mechanism will provide a good chance of revealing the mutual interplay between different biosynthetic routes, which could further help elucidate the mechanisms of effective composition accumulation in medicinal plants.
authors.affiliation: College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 6 Wangjing Zhonghuan Road, Beijing, 100102, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.forename: Xiaona
authors.initials: X
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Qiao
authors.forename: Yimei
authors.initials: Y
authors.lastname: Zang
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D000040:Abscisic Acid / Q000302:isolation & purification / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003517:Cyclopentanes / Q000302:isolation & purification / Q000378:metabolism; D005875:Gibberellins / Q000302:isolation & purification / Q000378:metabolism; D029882:Glycyrrhiza uralensis / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000302:isolation & purification / Q000378:metabolism; D054883:Oxylipins / Q000302:isolation & purification / Q000378:metabolism; D010937:Plant Growth Regulators / Q000302:isolation & purification / Q000502:physiology*; D018515:Plant Leaves / Q000378:metabolism*; D010946:Plants, Medicinal / Q000378:metabolism; D020156:Salicylic Acid / Q000302:isolation & purification / Q000378:metabolism; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry
chemical_list: D003517:Cyclopentanes; D005875:Gibberellins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; C030737:indoleacetic acid; D000040:Abscisic Acid; C007842:gibberellic acid; D020156:Salicylic Acid