title: Label-free in-situ monitoring of protein tyrosine nitration in blood by surface-enhanced Raman spectroscopy.
abstract: A novel label-free method for the in-situ monitoring of protein tyrosine nitration (PTN) was explored based on surface-enhanced Raman spectroscopy (SERS). Benefiting from the relative weak binding ability of sulfate to silver surface, the Raman signals of nitrated peptides were boosted well with sulfate-aggregated silver nanoparticles (Ag NPs). The distinction of the SERS spectra between non-nitrated peptides and nitrated peptides was obtained by directly comparing SERS bands at 330-400cm(-1), allowing the rapid identification of PTN. Furthermore, without any pretreatments, the established method was successfully applied in the rapid in-situ dynamic monitoring of the mimic hemin-catalyzed PTN process in synthetic peptide, bovine serum albumin (BSA), and original human blood serum samples. The results indicated that the proposed approach could be a promising in-situ label-free tool for observing PTN process, which may be quite helpful to deeply understand the mechanism of post-translation modification.
authors.affiliation: Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yuan-Ting
authors.initials: YT
authors.lastname: Li
authors.affiliation: Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China. Electronic address: daweili@ecust.edu.cn.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.affiliation: Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China. Electronic address: ytlong@ecust.edu.cn.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
mesh_terms: D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D009566:Nitrates / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D009566:Nitrates; D012834:Silver; D014443:Tyrosine
keywords: Label-free in-situ monitoring; Protein tyrosine nitration; Surface-enhanced Raman spectroscopy