title: The ABA-deficiency suppressor locus HAS2 encodes the PPR protein LOI1/MEF11 involved in mitochondrial RNA editing.
abstract: The hot ABA-deficiency suppressor2 (has2) mutation increases drought tolerance and the ABA sensitivity of stomata closure and seed germination. Here we report that the HAS2 locus encodes the mitochondrial editing factor11 (MEF11), also known as lovastatin insensitive1. has2/mef11 mutants exhibited phenotypes very similar to the ABA-hypersensitive mutant, hai1-1 pp2ca-1 hab1-1 abi1-2, which is impaired in four genes encoding type 2C protein phosphatases (PP2C) that act as upstream negative regulators of the ABA signaling cascade. Like pp2c, mef11 plants were more resistant to progressive water stress and seed germination was more sensitive to paclobutrazol (a gibberellin biosynthesis inhibitor) as well as mannitol and NaCl, compared with the wild-type plants. Phenotypic alterations in mef11 were associated with the lack of editing of transcripts for the mitochondrial cytochrome c maturation FN2 (ccmFN2) gene, which encodes a cytochrome c-heme lyase subunit involved in cytochrome c biogenesis. Although the abundance of electron transfer chain complexes was not affected, their dysfunction could be deduced from increased respiration and altered production of hydrogen peroxide and nitric oxide in mef11 seeds. As minor defects in mitochondrial respiration affect ABA signaling, this suggests an essential role for ABA in mitochondrial retrograde regulation.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Sechet
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Roux
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Plessis
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Effroy
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Frey
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: François
authors.initials: F
authors.lastname: Perreau
authors.affiliation: CEA Saclay, IBiTec-S, CNRS UMR 8221, Serv Bioenerget Biol Struct & Mécanisme, F-91191 Gif Sur Yvette, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Biniek
authors.affiliation: CEA Saclay, IBiTec-S, CNRS UMR 8221, Serv Bioenerget Biol Struct & Mécanisme, F-91191 Gif Sur Yvette, France.
authors.forename: Anja
authors.initials: A
authors.lastname: Krieger-Liszkay
authors.affiliation: Université d'Angers, UMR IRHS 1345, INRA, Agrocampus Ouest, F-49045 Angers, France.
authors.forename: David
authors.initials: D
authors.lastname: Macherel
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Helen M
authors.initials: HM
authors.lastname: North
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; Institut Jean-Pierre Bourgin, INRA, Bât 7, F-78026 Versailles Cedex, France. Electronic address: hakim.mireau@versailles.inra.fr.
authors.forename: Hakim
authors.initials: H
authors.lastname: Mireau
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; Institut Jean-Pierre Bourgin, INRA, Bât 2, F-78026 Versailles Cedex, France. Electronic address: annie.marion-poll@versailles.inra.fr.
authors.forename: Annie
authors.initials: A
authors.lastname: Marion-Poll
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000502:physiology*; D017393:RNA Editing / Q000235:genetics / Q000502:physiology*; D000077278:RNA, Mitochondrial; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C551730:MEF11 protein, Arabidopsis; D024101:Mitochondrial Proteins; D000077278:RNA, Mitochondrial; D016601:RNA-Binding Proteins; D012313:RNA; D000040:Abscisic Acid
keywords: RNA editing; abscisic acid; drought tolerance; germination; mitochondria; pentatricopeptide repeat protein