title: Development and validation of LC-MS/MS method for quantification of pseudolaric acid B from the root bark of Pseudolarix kaempferi in rat plasma: application to a pharmacokinetic study.
abstract: Pseudolaric acid B (PAB), which is the main biologically active diterpene acid of Pseudolarix kaempferi, has presented anti-fungal, anti-tumor, anti-fertility, and anti-tubulin activities. In this study, a sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method with multiple-reaction monitoring mode was employed for quantification of PAB in rat plasma. The calibration curve was linear over the range of 0.86-288 ng/mL with correlation coefficient (r) greater than 0.995 for PAB, and the lower limit of quantification was 0.86 ng/mL in rat plasma. The accuracy of PAB was between -9.1% and 7.0% relative error, and precision ranged from 1.2 to 10.6% relative standard deviation. This method was successfully applied to the pharmacokinetic studies of PAB in Sprague-Dawley rats. After single intravenous administration of 2.0, 4.0, and 8.0mg/kg PAB to rats, the t1/2 were (16.1 ± 5.6), (30.0 ± 13.7), and (27.4 ± 5.3)min, respectively. The pharmacokinetics (C(2min), AUC) of PAB within the used dosage range were in accordance with linear pharmacokinetic characteristics.
authors.affiliation: Department of Gastrointestinal Surgery, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Helei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, the Third Hospital of Jilin University, Changchun 130031, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cardiovascular Medicine, the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Gastrointestinal Surgery, the First Hospital of Jilin University, Changchun 130021, China. Electronic address: suojian198004@sina.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Suo
authors.affiliation: Department of Emergency Medicine, the First Hospital of Jilin University, Changchun 130021, China. Electronic address: zhangnan7412@sina.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D057230:Limit of Detection; D008297:Male; D028201:Pinaceae / Q000737:chemistry*; D024301:Plant Bark; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004224:Diterpenes; C058391:pseudolaric acid B
keywords: Liquid chromatography–tandem mass spectrometry; Pharmacokinetic study; Pseudolaric acid B; Pseudolarix kaempferi; Rat plasma