title: Alpha-1 antitrypsin, retinol binding protein and keratin 10 alterations in patients with psoriasis vulgaris, a proteomic approach.
abstract: OBJECTIVE
  Psoriasis is an autoimmune disease that appears on the skin. Although psoriasis is clinically and histologically well characterized, its pathogenesis is unknown in detail. The aims of this study were to evaluate the proteome of psoriatic patients' sera and to compare them with those of normal healthy human to find valuable biomarkers.
  
  
  METHODS
  In a case-control study, twenty cases of white patients with psoriasis vulgaris, 10 males and 10 females and sixteen healthy controls, 8 males and 8 females were enrolled in the study. The serum protein expression patterns obtained after depletion of albumin were compared by using two dimensional gel electrophoresis (2-DE) coupled to MALDI/TOF-TOF to identify disease associated proteins.
  
  
  RESULTS
  Differential expression of nine protein spots representing four unique proteins including alpha-1 antitrypsin, retinol binding protein, keratin 10 and an unknown protein (with pI 6.47 and molecular weight of 19941 Da), between psoriatic and healthy human serum were found. Furthermore, expression of four new alpha-1 antitrypsin isoforms with different molecular weight and isoelectric point were observed in psoriatic serums in this research for the first time.
  
  
  CONCLUSIONS
  A unique proteomic profiling with abnormal expression of alpha-1 antitrypsin and presence of keratin 10 in sera of psoriasis patients were observed that may constitute new and useful findings of psoriasis and offer a clue to a better understanding of the inflammatory pathway.
authors.affiliation: Cellular and Molecular Biology Research Center, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran ; Department of Biochemistry, Faculty of Science, University of Sistan & Baluchestan, Zahedan, Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Fattahi
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Sistan & Baluchestan, Zahedan, Iran ; Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Kazemipour
authors.affiliation: Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran ; Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Masood
authors.initials: M
authors.lastname: Sepehrimanesh
keywords: Alpha-1 antitrypsin; Keratin 10; Proteomics; Psoriasis; Retinol binding protein