title: Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.
abstract: Somatic cells can be reprogrammed into embryonic stem cells (ESCs) by nuclear transfer (NT-ESCs), or into induced pluripotent stem cells (iPSCs) by the "Yamanaka method." However, recent studies have indicated that mouse and human iPSCs are prone to epigenetic and transcriptional aberrations, and that NT-ESCs correspond more closely to ESCs derived from in vitro fertilized embryos than iPSCs. In addition, the procedure of NT-ESCs does not involve gene modification. Demonstration of generation of NT-ESCs using an easily-accessible source of adult cell types would be very important. Adipose tissue is a source of readily accessible donor cells and can be isolated from both males and females at different ages. Here we report that NT-ESCs can be generated from adipose tissue-derived cells (ADCs). At morphological, mRNA and protein levels, these NT-ESCs show classic ESC colonies, exhibit alkaline phosphatase (AP) activity, and display normal diploid karyotypes. Importantly, these cells express pluripotent markers including Oct4, Sox2, Nanog and SSEA-1. Furthermore, they can differentiate in vivo into various types of cells from 3 germinal layers by teratoma formation assays. This study demonstrates for the first time that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.
authors.affiliation: a State Key Laboratory of Oncogenes and Related Genes; Renji-MedX Stem Cell Research Center; Ren Ji Hospital; School of Medicine ; Shanghai Jiao Tong University ; Shanghai , P.R. China.
authors.forename: Yiren
authors.initials: Y
authors.lastname: Qin
authors.forename: Jilong
authors.initials: J
authors.lastname: Qin
authors.forename: Chikai
authors.initials: C
authors.lastname: Zhou
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Gao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism*; D065150:Cellular Reprogramming; D016256:Lewis X Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D016256:Lewis X Antigen; D014157:Transcription Factors
keywords: adipose-tissue derived cells; embryonic stem cells; somatic cell nuclear transfer