title: Dormancy activation mechanism of oral cavity cancer stem cells.
abstract: Radiotherapy and chemotherapy are targeted primarily at rapidly proliferating cancer cells and are unable to eliminate cancer stem cells in the G0 phase. Thus, these treatments cannot prevent the recurrence and metastasis of cancer. Understanding the mechanisms by which cancer stem cells are maintained in the dormant G0 phase, and how they become active is key to developing new cancer therapies. The current study found that the anti-cancer drug 5-fluorouracil, acting on the oral squamous cell carcinoma KB cell line, selectively killed proliferating cells while sparing cells in the G0 phase. Bisulfite sequencing PCR showed that demethylation of the Sox2 promoter led to the expression of Sox2. This then resulted in the transformation of cancer stem cells from the G0 phase to the division stage and suggested that the transformation of cancer stem cells from the G0 phase to the division stage is closely related to an epigenetic modification of the cell.
authors.affiliation: Center of Implant Dentistry, School of Stomatology, China Medical University, Shenyang, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhao
authors.forename: Dehao
authors.initials: D
authors.lastname: Shang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Chunfu
authors.initials: C
authors.lastname: Deng
authors.forename: Xinshan
authors.initials: X
authors.lastname: Jia
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D005472:Fluorouracil / Q000008:administration & dosage; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009055:Mouth / Q000187:drug effects / Q000473:pathology; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D016192:Resting Phase, Cell Cycle / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D005472:Fluorouracil