title: Adenosine receptor transcriptomic profile in cardiac tissue of a Zucker rat model.
abstract: To evaluate the possible variations of adenosine receptor (AR) profile together with TNF-α and IL-6 mRNA in cardiac tissue of obese Zucker rats (OZR) during fasting conditions (fc) and during the induction of acute hyperglycemia (AH). OZR (O, n=21) and age-matched lean control rats (CO, n=18) were studied during fc (COfc, n=8; Ofc, n=13) and during the induction of AH (COAH, n=10; OAH, n=8). The histopathologic analysis performed on O and CO heart samples did not show abnormalities of myocardial structure. The AR transcriptomic profile was analyzed in O and CO by real-time polymerase chain reaction (PCR) and a significantly lower mRNA expression was observed for A2AR in O with respect to CO (p=0.047), while a significant upregulation was observed for A3R in O with respect to CO (p=0.002). No significant differences between O and CO were observed for TNF-α or IL-6. Correlations were found between glycemia and A1R (p=0.03) and A2BR (p=0.002); total cholesterol and A2BR (p=0.02) and A3R (p=0.0002), as well as between IL-6 and A1R (p=0.05) and TNF-α and A2AR (p<0.0001). The modulation of ARs in these settings could represent a promising approach to pharmacological treatment, which must be supported by diet restrictions and physical exercise.
authors.affiliation: 1 CNR Institute of Clinical Physiology, Biochemical and Molecular Biology Laboratory, Laboratory of Cardiovascular Biochemistry , Pisa, Italy .
authors.forename: Manuela
authors.initials: M
authors.lastname: Cabiati
authors.forename: Benedetta
authors.initials: B
authors.lastname: Svezia
authors.forename: Maria Angela
authors.initials: MA
authors.lastname: Guzzardi
authors.forename: Letizia
authors.initials: L
authors.lastname: Mattii
authors.forename: Andrea
authors.initials: A
authors.lastname: D'Amico
authors.forename: Chiara
authors.initials: C
authors.lastname: Caselli
authors.forename: Tommaso
authors.initials: T
authors.lastname: Prescimone
authors.forename: Maria Aurora
authors.initials: MA
authors.lastname: Morales
authors.forename: Silvia
authors.initials: S
authors.lastname: Del Ry
mesh_terms: D000818:Animals; D005215:Fasting / Q000502:physiology*; D020869:Gene Expression Profiling; D006321:Heart / Q000502:physiology*; D006943:Hyperglycemia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011924:Rats, Zucker; D060888:Real-Time Polymerase Chain Reaction; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D018047:Receptors, Purinergic P1; D014409:Tumor Necrosis Factor-alpha