title: Proliferative role of TRAF4 in breast cancer by upregulating PRMT5 nuclear expression.
abstract: In this study, we examined protein arginine methyltransferase 5 (PRMT5) and tumor necrosis factor receptor-associated 4 (TRAF4) expression in breast cancer to find the interaction mechanism between the two. We examined TRAF4 and PRMT5 expression by immunohistochemistry and found that their expression is positively correlated in breast cancer. Besides, PRMT5 expression was significantly associated with histological type and tumor size (p < 0.05). PRMT5 nuclear expression was significantly associated with HER2 expression (p < 0.05). PRMT5 and TRAF4 were both overexpressed in breast cancer tissues and cells, and we found that PRMT5 binds to the zinc finger structures in TRAF4 by coimmunoprecipitation and Western blotting. We also tested the potential regulatory effect between TRAF4 and PRMT5. TRAF4 upregulated PRMT5 expression, which occurred predominantly in the nucleus, on which TRAF4 promotion of cell proliferation in breast cancer is mainly dependent. PRMT5 may play an important role in activation of the NF-κB signaling pathway.
authors.affiliation: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Hua-Yan
authors.initials: HY
authors.lastname: Ren
authors.forename: Juan
authors.initials: J
authors.lastname: Jin
authors.forename: Ai-Lian
authors.initials: AL
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Sun
authors.forename: Ke-Xin
authors.initials: KX
authors.lastname: Diao
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Wang
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Mi
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D011484:Protein-Arginine N-Methyltransferases / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D053449:TNF Receptor-Associated Factor 4 / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D053449:TNF Receptor-Associated Factor 4; C506367:TRAF4 protein, human; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases