title: The osteogenic properties of multipotent mesenchymal stromal cells in cultures on TiO₂ sol-gel-derived biomaterial.
abstract: The biocompatibility of the bone implants is a crucial factor determining the successful tissue regeneration. The aim of this work was to compare cellular behavior and osteogenic properties of rat adipose-derived multipotent stromal cells (ASCs) and bone marrow multipotent stromal cells (BMSCs) cultured on metallic substrate covered with TiO2 sol-gel-derived nanolayer. The morphology, proliferation rate, and osteogenic differentiation potential of both ASCs and BMSCs propagated on the biomaterials were examined. The potential for osteogenic differentiation of ASCs and BMSCs was determined based on the presence of specific markers of osteogenesis, that is, alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCL). Additionally, the concentration of calcium and phosphorus in extracellular matrix was determined using energy-dispersive X-ray spectroscopy (SEM-EDX). Obtained results showed that TiO2 layer influenced proliferation activity of ASCs, which manifested by shortening of population doubling time and increase of OPN secretion. However, characteristic features of cells morphology and growth pattern of cultures prompted us to conclude that ultrathin TiO2 layer might also enhance osteodifferentiation of BMSCs. Therefore in our opinion, both populations of MSCs should be used for biological evaluation of biomaterials compatibility, such results may enhance the area of investigations related to regenerative medicine.
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b, 50-631 Wroclaw, Poland ; Wrocław Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b, 50-631 Wroclaw, Poland ; Wrocław Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b, 50-631 Wroclaw, Poland ; Wrocław Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b, 50-631 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Siudzińska
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b, 50-631 Wroclaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Wrocław Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland ; Institute of Materials Science and Applied Mechanics, Wroclaw University of Technology, Smoluchowskiego 25, 50-370 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Donesz-Sikorska
authors.affiliation: Institute of Materials Science and Applied Mechanics, Wroclaw University of Technology, Smoluchowskiego 25, 50-370 Wroclaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Krzak
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium