title: Post-transplant soluble CD30 levels are associated with early subclinical rejection in kidney transplantation.
abstract: Several studies have shown association of high pre- or post-transplant levels of soluble CD30 (sCD30) with acute rejection and poor late kidney transplant outcome. Our goal was to investigate whether sCD30 levels at month-3 post-transplant are associated with subclinical rejection, presence of CD30(+) cells within the graft, and expression of immune response genes in peripheral blood mononuclear cells. The study comprised 118 adult first kidney graft recipients, transplanted at a single center, receiving tacrolimus in low concentration. All were submitted to a protocol biopsy at month-3. Subclinical rejection was identified in 10 biopsies and sCD30 levels ≥ 61.88 ng/mL (P = 0.004), younger recipient age (P = 0.030) and non-Caucasian ethnicity (P = 0.011) were independently associated with this outcome. Rare CD30(+) cells were present in only two biopsies. There was a correlation between sCD30 levels and CD30 gene expression in peripheral blood mononuclear cells (r = 0.385, P = 0.043). These results show that high sCD30 levels are independent predictors of graft dysfunction and may contribute to patient selection protocols by indicating those who could benefit from a more thorough evaluation.
authors.affiliation: Instituto de Imunogenética-AFIP, Rua Loefgreen 1235, 04040-031 São Paulo, SP, Brazil; Universidade Federal de São Paulo, Rua Sena Madureira 1500, 04021-001 São Paulo, SP, Brazil. Electronic address: patricia.grenzi@igen.epm.br.
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Grenzi
authors.affiliation: Instituto de Imunogenética-AFIP, Rua Loefgreen 1235, 04040-031 São Paulo, SP, Brazil; Universidade Federal de São Paulo, Rua Sena Madureira 1500, 04021-001 São Paulo, SP, Brazil. Electronic address: erika@igen.epm.br.
authors.forename: Érika F
authors.initials: ÉF
authors.lastname: Campos
authors.affiliation: Hospital do Rim e Hipertensão, Rua Borges Lagoa 960, 04038-002 São Paulo, SP, Brazil. Electronic address: heliotedesco@medfarm.com.br.
authors.forename: Hélio T
authors.initials: HT
authors.lastname: Silva
authors.affiliation: Hospital do Rim e Hipertensão, Rua Borges Lagoa 960, 04038-002 São Paulo, SP, Brazil. Electronic address: claudiafelipe@medfarm.com.br.
authors.forename: Claudia R
authors.initials: CR
authors.lastname: Felipe
authors.affiliation: Universidade Federal de São Paulo, Rua Sena Madureira 1500, 04021-001 São Paulo, SP, Brazil. Electronic address: m.franco@unifesp.br.
authors.forename: Marcelo F
authors.initials: MF
authors.lastname: Franco
authors.affiliation: Universidade Federal do Paraná, Rua XV de Novembro 1299, 80060-000 Curitiba, PR, Brazil. Electronic address: mafer.soares@gmail.com.
authors.forename: Maria F
authors.initials: MF
authors.lastname: Soares
authors.affiliation: Universidade Federal de São Paulo, Rua Sena Madureira 1500, 04021-001 São Paulo, SP, Brazil; Hospital do Rim e Hipertensão, Rua Borges Lagoa 960, 04038-002 São Paulo, SP, Brazil. Electronic address: medina@hrim.com.br.
authors.forename: José O
authors.initials: JO
authors.lastname: Medina-Pestana
authors.affiliation: Instituto de Imunogenética-AFIP, Rua Loefgreen 1235, 04040-031 São Paulo, SP, Brazil; Universidade Federal de São Paulo, Rua Sena Madureira 1500, 04021-001 São Paulo, SP, Brazil. Electronic address: gerbase@igen.epm.br.
authors.forename: Maria
authors.initials: M
authors.lastname: Gerbase-DeLima
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology / Q000473:pathology; D006801:Humans; D017730:Ki-1 Antigen / Q000097:blood* / Q000276:immunology; D016030:Kidney Transplantation; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D013997:Time Factors
chemical_list: D017730:Ki-1 Antigen
keywords: Kidney transplantation; Post-transplant monitoring; Protocol biopsy; Subclinical rejection; sCD30