title: Low-expressed testis-specific calcium-binding protein CBP86-IV (CABYR) is observed in idiopathic asthenozoospermia.
abstract: OBJECTIVE
  To investigate the expression level of testis-specific calcium-binding protein CBP86-IV in normal and asthenozoospermic human sperm.
  
  
  METHODS
  The total RNA was extracted from human sperm, and target cDNA was obtained by reverse transcription-polymerase chain reaction. Then the cDNA was used for quantitative PCR analysis and cloned into the prokaryotic expression vector pET-28a, respectively. The fusion protein was induced and expressed as inclusion body which was used to produce the polyclonal antibody against TSCBP86-IV. The protein expression level of TSCBP86-IV from normal human sperm and idiopathic asthenozoospermic samples was detected by the purified antibody.
  
  
  RESULTS
  The experimental results showed that the protein expression of TSCBP86-IV was reduced in idiopathic asthenozoospermia and consistent with the transcriptional changing tendency which was detected by quantitative PCR analysis.
  
  
  CONCLUSIONS
  The stable and reliable change of TSCBP86-IV may be taken as a new molecular marker for clinical diagnosis of idiopathic asthenozoospermia.
authors.affiliation: Department of Andrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China, shenforest@163.com.
authors.forename: Shulin
authors.initials: S
authors.lastname: Shen
authors.forename: Jinzi
authors.initials: J
authors.lastname: Wang
authors.forename: Jihong
authors.initials: J
authors.lastname: Liang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000175:diagnosis* / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013081:Sperm Motility; D013737:Testis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C448922:CABYR protein, human; D002135:Calcium-Binding Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger