title: 24-chromosome PCR for aneuploidy screening.
abstract: OBJECTIVE
  The purpose of this study is to describe the many advantages of PCR-based comprehensive chromosome screening (CCS) methodologies and the importance of extensive preclinical validation.
  
  
  RESULTS
  The rigorous preclinical validation of quantitative real-time (q)PCR-based CCS involved an initial validation on cell lines, followed by a blinded evaluation on embryos. Comparison with alternative platforms and a prospective randomized clinical trial demonstrate superior precision and improved sustained implantation and delivery rates. Preclinical validation of targeted PCR-based next-generation sequencing (NGS) has also demonstrated consistency in positive controls, equivalent accuracy to commonly used techniques, high resolution, increased throughput, the simultaneous detection of single gene disorders and triploidy, and the potential to decrease costs. Prospective randomized controlled trials are ongoing to validate this technique for clinical use.
  
  
  CONCLUSIONS
  CCS using PCR-based methodology improves implantation and delivery rates and allows for fresh embryo transfers. Other platforms are available to select euploid embryos; however, there are meaningful differences between techniques. PCR-based NGS technology may further enhance the utility of CCS for patients with infertility.
authors.affiliation: aRobert Wood Johnson Medical School of Rutgers University, New Brunswick bReproductive Medicine Associates of New Jersey and Rutgers-Robert Wood Johnson Medical School, Basking Ridge, New Jersey, USA.
authors.forename: Marie D
authors.initials: MD
authors.lastname: Werner
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
mesh_terms: D000782:Aneuploidy; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation / Q000502:physiology; D004624:Embryo Transfer / Q000379:methods* / Q000639:trends; D005260:Female; D005820:Genetic Testing / Q000379:methods* / Q000639:trends; D059014:High-Throughput Nucleotide Sequencing / Q000639:trends; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000639:trends; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D017422:Sequence Analysis, DNA