title: Inactivation of BRD7 results in impaired cognitive behavior and reduced synaptic plasticity of the medial prefrontal cortex.
abstract: BRD7 is a bromodomain-containing protein (BCP), and recent evidence implicates the role of BCPs in the initiation and development of neurodevelopmental disorders. However, few studies have investigated the biological functions of BRD7 in the central nervous system. In our study, BRD7 was found to be widely expressed in various regions of the mouse brain, including the medial prefrontal cortex (mPFC), caudate putamen (CPu), hippocampus (Hip), midbrain (Mb), cerebellum (Cb), and mainly co-localized with neuron but not with glia. Using a BRD7 knockout mouse model and a battery of behavioral tests, we report that disruption of BRD7 results in impaired cognitive behavior leaving the emotional behavior unaffected. Moreover, a series of proteins involved in synaptic plasticity were decreased in the medial prefrontal cortex and there was a concomitant decrease in neuronal spine density and dendritic branching in the medial prefrontal cortex. However, no significant difference was found in the hippocampus compared to the wild-type mice. Thus, BRD7 might play a critical role in the regulation of synaptic plasticity and affect cognitive behavior.
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan Province 410013, People's Republic of China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Cao
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China. Electronic address: zhouming2001@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Tongzipo Road 172, Changsha, Hunan Province 410013, People's Republic of China.
authors.forename: Changqi
authors.initials: C
authors.lastname: Li
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Heran
authors.initials: H
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Shihe
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Xinye
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Third Xiang-Ya Hospital, Central South University, Tongzipo Road 237, Changsha, Hunan Province 410013, People's Republic of China.
authors.forename: Xiayu
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Peng
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.affiliation: Mitchell Cancer Institute, University of South Alabama, Mobile, AL 36604, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Tan
authors.affiliation: Cancer Research Institute, Central South University, Xiangya Road 110 Changsha, Hunan Province 410078, People's Republic of China. Electronic address: ligy@xysm.net.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D001007:Anxiety / Q000473:pathology / Q000503:physiopathology; D015153:Blotting, Western; D002868:Chromosomal Proteins, Non-Histone / Q000172:deficiency* / Q000235:genetics; D003072:Cognition Disorders / Q000473:pathology / Q000503:physiopathology*; D003712:Dendrites / Q000473:pathology / Q000502:physiology; D003863:Depression / Q000473:pathology / Q000503:physiopathology; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000502:physiology*; D009483:Neuropsychological Tests; D055696:Olfactory Perception / Q000502:physiology; D046709:Pattern Recognition, Physiological / Q000502:physiology; D017397:Prefrontal Cortex / Q000473:pathology / Q000503:physiopathology*; D021641:Recognition, Psychology / Q000502:physiology; D012938:Social Perception; D000092468:Bromodomain Containing Proteins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C400206:Brd7 protein, mouse; D000092468:Bromodomain Containing Proteins
keywords: BRD7; Cognitive impairment; Knockout mouse; Medial prefrontal cortex; Synaptic plasticity