title: Anti-cancer evaluation of quercetin embedded PLA nanoparticles synthesized by emulsified nanoprecipitation.
abstract: This study was carried out to synthesize quercetin (Qt) embedded poly(lactic acid) (PLA) nanoparticles (PLA-Qt) and to evaluate anti-cancer efficacy of PLA-Qt by using human breast cancer cells. PLA-Qt were synthesized by using novel emulsified nanoprecipitation technique with varying dimension of 32 ± 8 to 152 ± 9 nm of PLA-Qt with 62 ± 3% (w/w) entrapment efficiency by varying the concentration of polymer, emulsifier, drug and preparation temperature. The dimension of PLA-Qt was measured through transmission electron microscopy indicating larger particle size at higher concentration of PLA. The release rate of Qt from PLA-Qt was found to be more sustained for larger particle dimension (152 ± 9 nm) as compared to smaller particle dimension (32 ± 8 nm). Interaction between Qt and PLA was verified through spectroscopic and calorimetric methods. Delayed diffusion and stronger interaction in PLA-Qt caused the sustained delivery of Qt from the polymer matrix. In vitro cytotoxicity study indicate the killing of ∼ 50% breast cancer cells in two days at 100 μg/ml of drug concentration while the ∼ 40% destruction of cells require 5 days for PLA-Qt (46 ± 6 nm; 20mg/ml of PLA). Thus our results propose anticancer efficacy of PLA-Qt nanoparticles in terms of its sustained release kinetics revealing novel vehicle for the treatment of cancer.
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India. Electronic address: sanjeevbiochem11@gmail.com.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Pandey
authors.affiliation: School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Patel
authors.affiliation: Cell Death Research Laboratory, Division of Endocrinology, Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Thakur
authors.affiliation: Cell Death Research Laboratory, Division of Endocrinology, Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Durga P
authors.initials: DP
authors.lastname: Mishra
authors.affiliation: School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.
authors.forename: Pralay
authors.initials: P
authors.lastname: Maiti
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India. Electronic address: chaldar2001@yahoo.com.
authors.forename: Chandana
authors.initials: C
authors.lastname: Haldar
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D011232:Chemical Precipitation; D004347:Drug Interactions; D004655:Emulsions / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D004655:Emulsions; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D011142:Polyvinyl Alcohol; D011794:Quercetin
keywords: Nanoparticles; PLA; Quercetin