title: Hepatoprotective and in vivo antioxidant activity of Olax subscorpioidea Oliv. (Olacaceae) and Distemonathus benthamianus Baill. (Caesalpiniaceae).
abstract: BACKGROUND
  Liver diseases are on rise and remain a serious health problem. Olax subscorpioidea and Distemonanthus benthamianus are two plants used in Ivorian traditional medicine in the treatment of many diseases including jaundice and hepatitis.
  
  
  OBJECTIVE
  The present study was carried out to assess the hepatoprotective and in vivo antioxidant potentials of the hydro-ethanolic leaf extracts of these plants in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats.
  
  
  METHODS
  The plant extracts at doses of 25 and 100 mg/kg bw, and silymarin (25 mg/kg bw) were intraperitoneally (i.p.) injected once daily for 7 days to different groups of rats. Hepatotoxicity was induced on the 7(th) day in all the group animals except control. Rats were sacrificed on the 8(th) day and blood was collected. Serum biochemical parameters and antioxidant activity were measured using standard procedures. Histopathological examinations of liver rats were also performed.
  
  
  CONCLUSIONS
  Hepatotoxicity induced with CCl4 was well manifested by significant increase in serum activities of GOT, GPT, ALP and GGT, and enhancement of total bilirubin and TBARS levels. On the other hand, the level of total protein, albumin, α1-globulin significantly decreased, and DPPH-free radical scavenging activity and TAP were lowered. Pretreatment with plant extracts and silymarin prevent the toxic effects of CCl4 by decreasing serum enzyme activities, total bilirubin and TBARS levels and improving serum TAP and DPPH-free radical scavenging potential. Histopathological observations showed almost normal hepatic cells with a mild degree of inflammation, lesser fatty infiltration and absence of necrosis among the rats treated at 100 mg/kg of extracts of both the plants.
  
  
  CONCLUSIONS
  The results suggest that the hydro-ethanolic leaf extracts of O. subscorpioidea and D. benthamianus possesses hepatoprotective and in vivo antioxidant activity.
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemical Pharmacodynamy, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, 22 BP 582 Abidjan 22, Republic of Côte d'Ivoire.
authors.forename: Kouassi
authors.initials: K
authors.lastname: Konan
authors.affiliation: Department of Anatomy, Pathological Anatomy Cytology, Histology-Embryology and Cytogenetics, Laboratory of pathological anatomy, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, 22 BP 582 Abidjan 22, Republic of Côte d'Ivoire.
authors.forename: N'dah Kouamé
authors.initials: NK
authors.lastname: Justin
authors.affiliation: Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Republic of Côte d'Ivoire.
authors.forename: Boyvin
authors.initials: B
authors.lastname: Lydie
authors.affiliation: Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Republic of Côte d'Ivoire.
authors.forename: Méité
authors.initials: M
authors.lastname: Souleymane
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemical Pharmacodynamy, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, 22 BP 582 Abidjan 22, Republic of Côte d'Ivoire.
authors.forename: Yapo Adou
authors.initials: YA
authors.lastname: Francis
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemical Pharmacodynamy, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, 22 BP 582 Abidjan 22, Republic of Côte d'Ivoire.
authors.forename: N'guessan Jean
authors.initials: NJ
authors.lastname: David
keywords: Antioxidant activity; Distemonanthus benthamianus; Olax subscorpioidea; carbon tetrachloride; hepatoprotective activity; histopathology