title: Anaerosolibacter carboniphilus gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil.
abstract: A strictly anaerobic, mesophilic, iron-reducing bacterial strain, IRF19(T), was isolated from coal-contaminated soil in the Republic of Korea. IRF19(T) cells were straight, rod-shaped, Gram-staining-negative and motile by means of flagella. The optimum pH and temperature for their growth were determined to be pH 7.5-8.0 and 40 °C, while the optimum range was pH 6.5-10.0 and 20-45 °C, respectively. Strain IRF19(T) did not require NaCl for growth but it tolerated up to 2% (w/v). Growth was observed with yeast extract, D-glucose, D-fructose, D-ribose, D-mannitol, D-mannose, L-serine, L-alanine and L-isoleucine. Fe(III), elemental sulfur, thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain IRF19(T) is affiliated to the family Clostridiaceae and is most closely related to Salimesophilobacter vulgaris Zn2(T) (93.5% similarity), Geosporobacter subterraneus VNs68(T) (93.2%) and Thermotalea metallivorans B2-1(T) (92.3%). The major cellular fatty acids of strain IRF19(T) were C14 : 0, iso-C15 : 0 and C16 : 0, and the profile was distinct from those of the closely related species. The major respiratory quinone of strain IRF19(T) was menaquinone MK-5 (V-H2). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain IRF19(T) was determined to be 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic results, strain IRF19(T) is considered to represent a novel species of a novel genus of the family Clostridiaceae , for which we propose the name Anaerosolibacter carboniphilus gen. nov., sp. nov., with the type strain IRF19(T) ( =KCTC 15396(T) =JCM 19988(T)).
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Heeji
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: So-Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Ui-Gi
authors.initials: UG
authors.lastname: Min
authors.affiliation: Microbial Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: University of Science and Technology, Yuseong-gu, Daejeon 305-850, Republic of Korea.|Microbial Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jong-Geol
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jeong-Geol
authors.initials: JG
authors.lastname: Na
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003032:Coal Mining; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000737:chemistry; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D007501:Iron