title: Identification and characterization of enolase as a collagen-binding protein in Lactobacillus plantarum.
abstract: Collagen is a target of pathogens for adhesion, colonization, and invasion of host tissue. Probiotic bacteria can mimic the same mechanism as used by the pathogens in the colonization process, expressing cell surface proteins that specifically interact with extracellular matrix component proteins. The capability to bind collagen is expressed by several Lactobacillus isolates, including some Lactobacillus plantarum strains. In this study we report the involvement of the L. plantarum EnoA1 alfa-enolase in type I collagen (CnI) binding. By adhesion assays, we show that the mutant strain LM3-CC1, carrying a null mutation in the enoA1 gene, binds to immobilized collagen less efficiently than wild type strain. CnI overlay assay and Elisa tests, performed on the purified EnoA1, show that this protein can bind collagen both under denaturing and native conditions. By using truncated recombinant enolase proteins, we also show that the region spanning from 73rd to the 140th amino acid residues is involved in CnI binding.
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Salzillo
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Vastano
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Ugo
authors.initials: U
authors.lastname: Capri
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Lidia
authors.initials: L
authors.lastname: Muscariello
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Sacco
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Caserta, Italy.
authors.forename: Rosangela
authors.initials: R
authors.lastname: Marasco
mesh_terms: D001422:Bacterial Adhesion; D003094:Collagen / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D008565:Membrane Proteins; D003094:Collagen; D010751:Phosphopyruvate Hydratase
keywords: Adhesins; Collagen I; Enolase; Probiotics