title: Isolation and culturing of trophoblasts from human terminal placenta.
abstract: Trophoblast culture was derived from the islets of cell migrating from the fragments of placental microvilli. The isolated cells retained their cytotrophoblast phenotype longer (until passage 2-3) and then gained characteristics of proliferating mesenchymal cells. We conclude that changes in the morphology and co-expression of epithelial (cytokeratin 7) and mesenchymal (vimentin) markers attest to epithelial mesenchymal transition.
authors.affiliation: Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia, kolokoltd@mail.ru.
authors.forename: T D
authors.initials: TD
authors.lastname: Kolokol'tsova
authors.forename: N N
authors.initials: NN
authors.lastname: Saburina
authors.forename: P A
authors.initials: PA
authors.lastname: Poltavtseva
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology*