title: Ovarian Hormones Regulate SP-D Expression in the Mouse Uterus During Estrous Cycle and Early Pregnancy.
abstract: OBJECTIVE
  Differential expression of SP-D in the cycling human and mouse endometrium suggests its regulation by ovarian hormones.
  
  
  METHODS
  SP-D expression in the mouse uterus was analyzed across the estrous cycle and during early pregnancy. Effect of exogenous ovarian hormones on the uterine expression of SP-D was analyzed.
  
  
  RESULTS
  SP-D expression varied across the estrous cycle and peaked in the estrous phase. SP-D transcript levels increased by fourfold in the uteri of estrogen-treated mice while co-administration of estrogen and progesterone enhanced SP-D levels by ninefold. However, treatment with progesterone alone significantly downregulated SP-D expression. Diethylstilbestrol enhanced SP-D transcript levels in the uteri of immature mice by 10-fold. During pregnancy, SP-D levels declined rapidly from 0.5 dpc to 6.5 dpc. In silico analysis predicted the presence of two potential ERE and 1 PRE in the mouse SP-D gene promoter region.
  
  
  CONCLUSIONS
  Estrogen positively regulates expression of SP-D in the mouse uterus. Progesterone, along with estrogen synergizes SP-D expression, however, when administered alone results in negative regulation.
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai, India.
authors.forename: Sharon
authors.initials: S
authors.lastname: Kay
authors.affiliation: Experimental Animal Facility, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai, India.
authors.forename: Siddhanath Maruti
authors.initials: SM
authors.lastname: Metkari
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai, India.
authors.forename: Taruna
authors.initials: T
authors.lastname: Madan
mesh_terms: D000818:Animals; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism*; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism* / Q000601:surgery; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D037662:Pulmonary Surfactant-Associated Protein A / Q000096:biosynthesis; D037663:Pulmonary Surfactant-Associated Protein D / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
chemical_list: D004967:Estrogens; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol
keywords: Estrogen; progesterone; surfactant protein-A; surfactant protein-D