title: The galactose-binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines.
abstract: In this study, the amino acid sequence and anti-inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% - 3 h, 19% - 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF-α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate-binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF-α (68%) and IL1-β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti-inflammatory activity by inhibiting neutrophil migration by reducing TNF-α and IL1-β levels via the lectin domain.
authors.affiliation: Departamento de Morfologia, Universidade Federal do Ceará, Rua Delmiro de Farias s/n, 60430-170, Fortaleza, Ceará, Brazil.
authors.forename: Deysen Kerlla Fernandes Bezerra
authors.initials: DK
authors.lastname: Girão
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Alana
authors.initials: A
authors.lastname: de Freitas Pires
authors.forename: Timna Varela
authors.initials: TV
authors.lastname: Martins
authors.forename: Álvaro Xavier
authors.initials: ÁX
authors.lastname: Franco
authors.forename: Cecília Mendes
authors.initials: CM
authors.lastname: Morais
authors.forename: Kyria
authors.initials: K
authors.lastname: Santiago do Nascimento
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Helton Colares
authors.initials: HC
authors.lastname: da Silva
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Pedro Marcos Gomes
authors.initials: PM
authors.lastname: Soares
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D036481:Bauhinia / Q000737:chemistry*; D002448:Cell Adhesion; D016207:Cytokines / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D037161:Galectins / Q000737:chemistry / Q000494:pharmacology*; D036904:Leukocyte Rolling; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010538:Peritonitis / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D037161:Galectins; D010936:Plant Extracts; D037121:Plant Lectins
keywords: Bauhinia bauhinioides; TNF-α; anti-inflammatory effect; galactose-binding lectin; neutrophil