title: Combination treatment with whole body vibration and a kidney-tonifying herbal Fufang prevent osteoporosis in ovariectomized rats.
abstract: OBJECTIVE
  To assess the ability of whole body vibration (WBV) with the kidney-tonifying herbal Fufang (Bushen Zhuanggu Granules, BZG) to prevent osteoporosis in ovariectomized rats.
  
  
  METHODS
  Fifty 6-month-old female Sprague Dawley rats were divided into five groups: sham-operated (SHAM), ovariectomized (OVX), OVX with WBV (OVX + WBV), OVX with BZG (OVX + BZG), OVX with both WBV and BZG (OVX + WBV + BZG). The SHAM group received normal saline. After 12 weeks of treatment, the rats were killed, their serum concentrations of osteopontin (OPN), receptor activator of nuclear factor kappa-B ligand RANKL and bone turnover markers assayed and bone mineral density (BMD), histomorphometry and bone strength evaluated.
  
  
  RESULTS
  Concentrations of OPN were significantly lower in the SHAM, OVX + WBV and OVX + WBV + BZG groups at 12 weeks, whereas concentrations of RANKL had decreased significantly in the SHAM, OVX + WBV, OVX + BZG and OVX + WBV + BZG groups. In the OVX + WBV, OVX + BZG and OVX + WBV + BZG groups the amount of bone turnover had been significantly antagonized. Compared with OVX group, BMD, % trabecular area (Tb.Ar), number of trabeculae (Tb.N) and assessed biomechanical variables were higher in OVX+WBV group, whereas and BMD, %Tb.Ar, Tb.N, maximal load and yield load were higher in the OVX + BZG group. All tested indices were significantly lower in the OVX + WBV and OVX + BZG groups than in the OVX + WBV + BZG group.
  
  
  CONCLUSIONS
  Either WBV or BZG alone prevents OVX-induced bone loss. However, BZG enhances the effect of WBV by further enhancing BMD, bone architecture and strength.
authors.affiliation: Department of Rehabilitation, General Hospital of Guangzhou Military Command of People's Liberation Army, Guangzhou, China.
authors.forename: Qiu-shi
authors.initials: QS
authors.lastname: Wei
authors.forename: Hai-bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Jun-ling
authors.initials: JL
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Fang
authors.forename: Guang-quan
authors.initials: GQ
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Tan
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Deng
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D010024:Osteoporosis / Q000209:etiology / Q000517:prevention & control*; D010052:Ovariectomy; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014732:Vibration / Q000627:therapeutic use*
chemical_list: D050071:Bone Density Conservation Agents; D004365:Drugs, Chinese Herbal; C528816:bushen zhuanggu
keywords: Kidney-tonifying herbal; Osteopontin; Osteoporosis; Ovariectomy; Whole body vibration