title: Cytotoxicity of elastomeric power chains in artificial saliva: an in vitro study.
abstract: BACKGROUND
  The main aim of this paper is to investigate the cytotoxicity of elastomeric power chains after stretching and immersion in a solution of artificial saliva.
  
  
  METHODS
  Two brands of grey polyurethane power chains available from two different firms (GAC, G&H) were selected for cytotoxicity assay. Each segment was stretched up to an initial force of approximately 200 g. Then each segment was kept stretched using appropriate equipment. The samples were tested in artificial saliva at two pH levels, pH7 and pH4. Following incubation, the saliva was removed, filtered and placed in contact with cell culture media using the RD line.
  
  
  CONCLUSIONS
  At this supernatant dilution, no statistically significant difference was observed between the different groups of power chains studied in terms of cell viability. The GAC and G&H power chains, whether stretched or not and immerged in either pH7 or pH4 saliva, showed no toxic effect on RD cells (human cell line).
authors.affiliation: M.M.B Biotechnology and Biomechanics Research team, service d'orthopédie dento-faciale, centre hospitalier Ibn Sina, faculté de médecine dentaire, université Mohammed V - Souissi (UM5S), avenue Allal Al Fassi, rue Mohammed Jazouli, BP 6212, Rabat, Morocco. Electronic address: halimiali111@yahoo.fr.
authors.forename: Abdelali
authors.initials: A
authors.lastname: Halimi
authors.affiliation: M.M.B Biotechnology and Biomechanics Research team, service d'orthopédie dento-faciale, centre hospitalier Ibn Sina, faculté de médecine dentaire, université Mohammed V - Souissi (UM5S), avenue Allal Al Fassi, rue Mohammed Jazouli, BP 6212, Rabat, Morocco.
authors.forename: Mohamed-Faouzi
authors.initials: MF
authors.lastname: Azeroual
authors.affiliation: Département de biologie et immunologie, faculté des sciences, université Mohammed V - Agdal (UM5A), 4, avenue Ibn Battouta, BP 1014, Rabat, Morocco.
authors.forename: Latifa
authors.initials: L
authors.lastname: Eddimani
authors.affiliation: Département de génétique médicale, Institut national d'hygiène, université Mohammed V - Souissi (UM5S), 27, avenue Ibn Battouta, 10090 Rabat, Morocco.
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Natiq
authors.affiliation: Département de biologie et immunologie, faculté des sciences, université Mohammed V - Agdal (UM5A), 4, avenue Ibn Battouta, BP 1014, Rabat, Morocco.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Bakri
authors.affiliation: M.M.B Biotechnology and Biomechanics Research team, service d'orthopédie dento-faciale, centre hospitalier Ibn Sina, faculté de médecine dentaire, université Mohammed V - Souissi (UM5S), avenue Allal Al Fassi, rue Mohammed Jazouli, BP 6212, Rabat, Morocco.
authors.forename: Fatima
authors.initials: F
authors.lastname: Zaoui
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003764:Dental Materials / Q000737:chemistry / Q000633:toxicity*; D004548:Elasticity; D020724:Elastomers / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007101:Immersion; D008422:Materials Testing; D009967:Orthodontic Appliances; D011140:Polyurethanes / Q000737:chemistry / Q000633:toxicity*; D012464:Saliva, Artificial / Q000737:chemistry*; D013314:Stress, Mechanical; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003764:Dental Materials; D020724:Elastomers; D011140:Polyurethanes; D012464:Saliva, Artificial; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue
keywords: Biodegradation; Biomechanics; Cytotoxicity; Elastomeric power chain; In vitro study; RD cell line