title: TGF-β/Smad2/3 signal pathway involves in U251 cell proliferation and apoptosis.
abstract: TGF-β/Smad2/3 signal pathway is regarded as a central regulator in various tumors, but its roles in brain cancer therapy remain unknown. In this study, we identify that the TGF-β/Smad2/3 signal pathway is activated in human brain glioma cells; inhibitor (SB203580) and siRNA against Smad2/3 quickly inhibited the phosphorylation of Smad2 and 3, expression of its major downstream gene, Ki-67, arrested cells in the G2/M phase and induced apoptosis of cells. The findings suggest that TGF-β/Smad2/3 pathway plays a key role in U251 cell growth and metastasis, which suggests its potential role in the molecular therapy of brain cancer.
authors.affiliation: Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Treatment of Tianjin City, Tianjin 300060, China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Zhao
authors.affiliation: Department of Nephrology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Li
authors.affiliation: Tianjin Huanhu Hospital, Tianjin 300060, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Feng
authors.affiliation: Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin 300100, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China. Electronic address: yzhangtj@yeah.net.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Treatment of Tianjin City, Tianjin 300060, China.
authors.forename: Jin-Cheng
authors.initials: JC
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin 300060, China.
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D051899:Smad2 Protein / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D051900:Smad3 Protein / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
chemical_list: D007093:Imidazoles; D019394:Ki-67 Antigen; D011725:Pyridines; D034741:RNA, Small Interfering; C497782:SMAD2 protein, human; C497790:SMAD3 protein, human; D051899:Smad2 Protein; D051900:Smad3 Protein; D016212:Transforming Growth Factor beta; C093642:SB 203580
keywords: Apoptosis; Bcl-2; Caspase-3; TGF-β/Smad2/3