title: A label-free method for detecting biothiols based on poly(thymine)-templated copper nanoparticles.
abstract: A novel label-free nanosensor has been developed for detecting biothiols including cysteine, glutathione and homocysteine based on poly(thymine)-templated fluorescent copper nanoparticles (CuNPs), which were controlled through thymine-Hg(II)-thymine coordination. This assay provides a simple, cost-effective, and sensitive platform for the detection of biothiols. By employing this turn-on sensor, biological thiols, such as cysteine, glutathione and homocysteine, are successfully detected at concentrations as low as 12.5nM for cysteine, 15nM for glutathione, and 20nM for homocysteine. The sensing system also exhibits high selectivity against other amino acids, and the application of the sensor for biological fluids shows that the proposed method works well for real samples.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Qi-Yong
authors.initials: QY
authors.lastname: Cai
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: jiage0630@hnu.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Ge
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Dong
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: zhaohui.li@zzu.edu.cn.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
mesh_terms: D003300:Copper / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds / Q000097:blood*; D013941:Thymine / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D003300:Copper; D008628:Mercury; D013941:Thymine
keywords: Copper nanoparticles; Cysteine; Glutathione; Homocysteine; Label-free; Thymine-Hg(II)-thymine