title: Current approaches in SELEX: An update to aptamer selection technology.
abstract: Systematic evolution of ligands by exponential enrichment (SELEX) is a well-established and efficient technology for the generation of oligonucleotides with a high target affinity. These SELEX-derived single stranded DNA and RNA molecules, called aptamers, were selected against various targets, such as proteins, cells, microorganisms, chemical compounds etc. They have a great potential in the use as novel antibodies, in cancer theragnostics and in biomedical research. Vast interest in aptamers stimulated continuous development of SELEX, which underwent numerous modifications since its first application in 1990. Novel modifications made the selection process more efficient, cost-effective and significantly less time-consuming. This article brings a comprehensive and up-to-date review of recent advances in SELEX methods and pinpoints advantages, main obstacles and limitations. The post-SELEX strategies and examples of application are also briefly outlined in this review.
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: darmosta@vscht.cz.
authors.forename: Mariia
authors.initials: M
authors.lastname: Darmostuk
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: silvie.rimpelova@vscht.cz.
authors.forename: Silvie
authors.initials: S
authors.lastname: Rimpelova
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, Bratislava 811 08, Slovak Republic. Electronic address: helena.gbelcova@fmed.uniba.sk.
authors.forename: Helena
authors.initials: H
authors.lastname: Gbelcova
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: tomas.ruml@vscht.cz.
authors.forename: Tomas
authors.initials: T
authors.lastname: Ruml
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D019075:Electrophoresis, Capillary; D008280:Magnetics; D008863:Microspheres; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide
keywords: Aptamers; In silico selection; Oligonucleotide library; PCR; Post-SELEX; SELEX