title: [Correlation of autophagy-associated gene Atg5 with tumorigenesis of prostate cancer].
abstract: OBJECTIVE
  To investigate the correlation of the autophagy-associated gene Atg5 with the pathogenesis of prostate cancer.
  
  
  METHODS
  Using real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and immunohistochemistry, we detected the expression of Atg5 in 50 cases of prostate intraepithelial neoplasm (PIN), 69 cases of prostate cancer (PCa), and 30 cases of benign prostatic hyperplasia (BPH).
  
  
  RESULTS
  The expression level of Atg5 mRNA was significantly higher in PIN (5.270 ± 0.230) and PCa (5.131 ± 0.252) than in the BPH tissue (1.723 ± 0.017) (P <0.01), and so was the positive rate of the Atg5 expression in the patients of the PIN group (94%) and PCa group (88.4%) than in those of the BPH group (6.7%) (P<0.01), but with no statistically significant differences between the PIN and PCa groups (P >0.05). No significant correlation was observed between the expression of Atg5 and the Gleason score of PCa (P >0.05).
  
  
  CONCLUSIONS
  The upregulated expression of Atg5 might play a role in the tumorigenesis of prostate cancer.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Ci
authors.initials: C
authors.lastname: Li
authors.forename: Lu-hong
authors.initials: LH
authors.lastname: Zhu
mesh_terms: D000368:Aged; D001343:Autophagy; D000071187:Autophagy-Related Protein 5; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015854:Up-Regulation
chemical_list: C502778:ATG5 protein, human; D000071187:Autophagy-Related Protein 5; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger