title: Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures.
abstract: The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.
authors.affiliation: Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, 3012, Switzerland.
authors.forename: Hulda R
authors.initials: HR
authors.lastname: Jonsdottir
authors.forename: Ronald
authors.initials: R
authors.lastname: Dijkman
mesh_terms: D001980:Bronchi / Q000166:cytology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D017934:Coronavirus / Q000502:physiology*; D004847:Epithelial Cells / Q000821:virology*; D017930:Genes, Reporter; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D014132:Trachea / Q000166:cytology; D056189:Viral Tropism; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D049410:Luciferases, Renilla