title: In silico analysis for structure, function and T-cell epitopes of a hypothetical conserved (HP-C) protein coded by PVX_092425 in Plasmodium vivax.
abstract: OBJECTIVE
  Plasmodium spp. merozoite glycosylphosphatidylinositol-anchored proteins (GPI-APs) considered as protective immunogen in novel vaccines against malaria. To analyze the structure and function of a hypothetical conserved (HP-C) GPI-AP coded by gene PVX_092425 from Plasmodium vivax, and find its potential T-cell epitopes for further vivax malaria vaccine study.
  
  
  METHODS
  The structure, function and T-cell epitopes of the HP-C protein named Pvx_092425 were analyzed and predicted by online and offline bioinformatics software.
  
  
  RESULTS
  The bioinformatics data showed that the Pvx_092425 is an 830 amino acid (AA) long polypeptide encoded by five exons gene PVX_092425.It contains a pectin lyase-like superfamily, an AA repeats region, a cys-rich region and a transmembrane domain (TM) in C-terminal region. The alignment analysis drew it has a unique AA repeats region among Plasmodium spp. It was located in the cytoplasm, secretory system or cellular nucleus of P. vivax merozoite. For the sequence, the fragment of I823-V829 inserts in the interior side of the membrane, and M1--A812 belongs to the cytoplasmic tail. It has seven protein-protein binding sites. The peptides with the best predicted binding affinities were human leucocyte antigen (HLA) HLA-A*0203, HLA-DRB1*0101 and HLA- DRB1*0701.Among these predicted peptides, 582FLWDKALFD590 epitope interacted with HLA-DRB1*0101 allele showed best binding affinity compared to others. Structural analysis explained that the epitope fits well into the epitope-binding groove of HLA-DRB1*0101.
  
  
  CONCLUSIONS
  It proposes that the Pvx_092425 plays a key role during erythrocyte stage and generates information that is useful for development of blood-stage vaccine to block the merozoites invasion.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Mo
authors.forename: Jian
authors.initials: J
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000235:genetics; D000953:Antigens, Protozoan / Q000235:genetics; D019295:Computational Biology; D003198:Computer Simulation; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology; D006790:Host-Parasite Interactions; D006801:Humans; D016780:Malaria, Vivax / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D010966:Plasmodium vivax / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics; D012156:Reticulocytes / Q000473:pathology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D018984:Epitopes, T-Lymphocyte; D008565:Membrane Proteins; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins
keywords: Glycosylphosphatidylinositol,; Human leucocyte antigen,; Hypothetical conserved,; Molecular docking,; Plasmodium vivax,; T-cell epitope