title: Unexplored Brazilian oceanic island host high salt tolerant biosurfactant-producing bacterial strains.
abstract: We aimed to isolate biosurfactant-producing bacteria in high salt conditions from uncontaminated soils on the Brazilian oceanic island, Trindade. Blood agar medium was used for the isolation of presumptive biosurfactant-producing bacteria. Confirmation and measurements of biosurfactant production were made using an oil-spreading method. The isolates were identified by fatty acid profiles and partial 16S rRNA gene sequence analysis. A total of 14 isolates obtained from the 12 soil samples were found to produce biosurfactants. Among them, two isolates stood out as being able to produce biosurfactant that is increasingly active in solutions containing up to 175 g L(-1) NaCl. These high salt tolerant biosurfactant producers are affiliated to different species of the genus Bacillus. Soil organic matter showed positive correlation with the number of biosurfactant-producing bacteria isolated from our different sampling sites. The applied approach successfully recovered and identified biosurfactant-producing bacteria from non-contaminated soils. Due to the elevated salt tolerance, as well as their capacity to produce biosurfactants, these isolates are promising for environmental biotechnological applications, especially in the oil production chain.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Av. P.H. Rolfs, S/N, Campus, Viçosa, MG, CEP 36570-000, Brazil.
authors.forename: Fábio Sérgio Paulino
authors.initials: FS
authors.lastname: da Silva
authors.forename: Victor Satler
authors.initials: VS
authors.lastname: Pylro
authors.forename: Pericles Leonardo
authors.initials: PL
authors.lastname: Fernandes
authors.forename: Gisele Souza
authors.initials: GS
authors.lastname: Barcelos
authors.forename: Karlos Henrique Martins
authors.initials: KH
authors.lastname: Kalks
authors.forename: Carlos Ernesto Gonçalves Reynaud
authors.initials: CE
authors.lastname: Schaefer
authors.forename: Marcos Rogério
authors.initials: MR
authors.lastname: Tótola
mesh_terms: D001407:Bacillus / Q000302:isolation & purification* / Q000378:metabolism; D062312:Islands; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000032:analysis; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D012987:Soil; D013501:Surface-Active Agents; D012965:Sodium Chloride