title: Cascade enzymatic reactions for efficient carbon sequestration.
abstract: Thermochemical processes developed for carbon capture and storage (CCS) offer high carbon capture capacities, but are generally hampered by low energy efficiency. Reversible cascade enzyme reactions are examined in this work for energy-efficient carbon sequestration. By integrating the reactions of two key enzymes of RTCA cycle, isocitrate dehydrogenase and aconitase, we demonstrate that intensified carbon capture can be realized through such cascade enzymatic reactions. Experiments show that enhanced thermodynamic driving force for carbon conversion can be attained via pH control under ambient conditions, and that the cascade reactions have the potential to capture 0.5 mol carbon at pH 6 for each mole of substrate applied. Overall it manifests that the carbon capture capacity of biocatalytic reactions, in addition to be energy efficient, can also be ultimately intensified to approach those realized with chemical absorbents such as MEA.
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Frigo-Vaz
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Wenyun
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA. Electronic address: ping@umn.edu.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000154:Aconitate Hydratase / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D057965:Carbon Sequestration; D004798:Enzymes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D004798:Enzymes; D002244:Carbon; D007521:Isocitrate Dehydrogenase; D000154:Aconitate Hydratase
keywords: Aconitase; Biocatalysis; Carbon capture and storage; Carbon dioxide; Isocitrate dehydrogenase