title: Synthesis and cellular internalization of spindle hematite/polymer hybrid nanoparticles.
abstract: Nonspherical spindle-shaped hematite/polymer hybrid nanoparticles (SPNPs) were synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP). The long axis of the SPNPs was 370 ± 65 nm, and the short axis was 80 ± 15 nm with an aspect ratio of 4.6-4.7. The SPNPs were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). Thermogravimetric analysis (TGA) was used to estimate the content of grafted polymer. Light-scattering measurement was used to detect the particle size distribution of SPNPs in water and in cell culture medium. HeLa cells internalized the SPNPs within 1 h, and the uptake reached equilibrium in 8 h. These observations contribute to better understanding of the interactions between nonspherical nanoparticles and cells, which may have implication for designing drug delivery vehicles.
authors.affiliation: Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Lixin
authors.initials: L
authors.lastname: Liu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D005290:Ferric Compounds; D011108:Polymers; D012235:Rhodamines; C000499:ferric oxide; D012822:Silicon Dioxide; C029773:rhodamine B
keywords: HeLa cells; cellular internalization; grafted polymers; hematite nanoparticles; nonspherical particles