title: Hepatoprotective, Antioxidant, and Anticancer Effects of the Tragopogon porrifolius Methanolic Extract.
abstract: Tragopogon porrifolius (Asteraceae), commonly referred to as white salsify, is an edible herb used in Lebanese folk medicine to treat cancer and liver dysfunction. In this study, we investigated the antioxidant activity of Tragopogon porrifolius methanolic extract, both in vitro and in vivo, in addition to its hepatoprotective and anticancer activities. Total phenolic and flavonoid contents were measured and found to be 37.0 ± 1.40 mg GAE/g and 16.6 ± 0.42 mg QE/g dry weight, respectively. In vitro antioxidant assays revealed an FRAP value of 659 ± 13.8 µmol Fe(2+)/g of extract and DPPH IC50 value 15.2 µg/mL. In rats subjected to CCl4-induced hepatotoxicity, significant increase in CAT, SOD, and GST levels was detected. The highest dose of the extract (250 mg/kg) recorded a fold increase of 1.68 for SOD, 2.49 for GST, and 3.2 for CAT. The extract also showed substantial decrease in AST (57%), ALT (56%), and LDH (65%) levels. Additionally, the extract caused a dose-dependent decrease in cell viability and proliferation. In conclusion, the methanolic extract of T. porrifolius displayed a relatively high antioxidant activity both in vitro and in vivo as well as hepatoprotective potential against liver toxicity in rats and anticancer effect on MDA-MB-231 and Caco-2 cells.
authors.affiliation: Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 36, Byblos 1401, Lebanon.
authors.forename: Clara
authors.initials: C
authors.lastname: Tenkerian
authors.affiliation: Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 36, Byblos 1401, Lebanon.
authors.forename: Mirvat
authors.initials: M
authors.lastname: El-Sibai
authors.affiliation: Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 36, Byblos 1401, Lebanon.
authors.forename: Costantine F
authors.initials: CF
authors.lastname: Daher
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, Lebanese American University, P.O. Box 36, Byblos 1401, Lebanon.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Mroueh