title: Protective effects of aerobic swimming training on high-fat diet induced nonalcoholic fatty liver disease: regulation of lipid metabolism via PANDER-AKT pathway.
abstract: This study aimed to investigate the mechanism by which aerobic swimming training prevents high-fat-diet-induced nonalcoholic fatty liver disease (NAFLD). Forty-two male C57BL/6 mice were randomized into normal-diet sedentary (ND; n = 8), ND exercised (n = 8), high-fat diet sedentary (HFD; n = 13), and HFD exercised groups (n = 13). After 2 weeks of training adaptation, the mice were subjected to an aerobic swimming protocol (60 min/day) 5 days/week for 10 weeks. The HFD group exhibited significantly higher mRNA levels of fatty acid transport-, lipogenesis-, and β-oxidation-associated gene expressions than the ND group. PANDER and FOXO1 expressions increased, whereas AKT expression decreased in the HFD group. The aerobic swimming program with the HFD reversed the effects of the HFD on the expressions of thrombospondin-1 receptor, liver fatty acid-binding protein, long-chain fatty-acid elongase-6, Fas cell surface death receptor, and stearoyl-coenzyme A desaturase-1, as well as PANDER, FOXO1, and AKT. In the HFD exercised group, PPARα and AOX expressions were much higher. Our findings suggest that aerobic swimming training can prevent NAFLD via the regulation of fatty acid transport-, lipogenesis-, and β-oxidation-associated genes. In addition, the benefits from aerobic swimming training were achieved partly through the PANDER-AKT-FOXO1 pathway.
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Immunology, Liaoning Medical University, Jinzhou, Liaoning, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Jin
authors.affiliation: Department of Neurobiology, Liaoning Medical University, Jinzhou, Liaoning, China.
authors.forename: Donghe
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Physiology, Liaoning Medical University, Jinzhou, Liaoning, China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, China.
authors.forename: Xifan
authors.initials: X
authors.lastname: Mei
authors.affiliation: Department of Physiology and Pathophysiology, Peking University Diabetes Center, Peking University Health Science Center, Beijing, China; Shenzhen University Diabetes Center, Shenzhen University Health Science Center, Shenzhen, China.
authors.forename: Youfei
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning, China. Electronic address: cliuxmei@outlook.com.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D050356:Lipid Metabolism; D050155:Lipogenesis; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011897:Random Allocation; D015398:Signal Transduction; D013550:Swimming
chemical_list: D016207:Cytokines; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; C504749:PANDER protein, mouse; D051057:Proto-Oncogene Proteins c-akt
keywords: Exercise; FOXO1; Fatty acid transport; Fatty acid β-oxidation; Lipogenesis; PPARα