title: [Stress proteins in the cells of Porphyra purpurea (Rhodophyta) thallus].
abstract: Heat shock proteins have been revealed for the first time by the methods of Western blotting using alkaline phosphatase and ECL in the cells of Porphyra purpurea from Kattegat area of the Baltic Sea in normal and experimental stress conditions. It was demonstrated with application of monoclonal anti-Hsp70 antibodies that a slight band about 70 kDa is present constitutively at the film; additionally the polypeptide of about 40 kDa ("Hsp40") has been detected. After heat shock at 28 degrees C during 1 hr significant "expenditure" of Hsp70 was observed, as well as the pronounced induction of "Hsp40"; the induction was expressed especially strongly in 24 hr after the stress application.
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Podlipaeva
authors.forename: S
authors.initials: S
authors.lastname: Ful'da
authors.forename: A V
authors.initials: AV
authors.lastname: Gudkov
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D020418:Algal Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D000469:Alkaline Phosphatase / Q000737:chemistry; D015153:Blotting, Western; D015870:Gene Expression; D050956:HSP40 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006358:Hot Temperature; D008163:Luminescent Measurements; D044664:Porphyra / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D020418:Algal Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D000469:Alkaline Phosphatase