title: Assessing the antimicrobial activity of polyisoprene based surfaces.
abstract: There has been an intense research effort in the last decades in the field of biofouling prevention as it concerns many aspects of everyday life and causes problems to devices, the environment, and human health. Many different antifouling and antimicrobial materials have been developed to struggle against bacteria and other micro- and macro-organism attachment to different surfaces. However the "miracle solution" has still to be found. The research presented here concerns the synthesis of bio-based polymeric materials and the biological tests that showed their antifouling and, at the same time, antibacterial activity. The raw material used for the coating synthesis was natural rubber. The polyisoprene chains were fragmented to obtain oligomers, which had reactive chemical groups at their chain ends, therefore they could be modified to insert polymerizable and biocidal groups. Films were obtained by radical photopolymerization of the natural rubber derived oligomers and their structure was altered, in order to understand the mechanism of attachment inhibition and to increase the efficiency of the anti-biofouling action. The adhesion of three species of pathogenic bacteria and six strains of marine bacteria was studied. The coatings were able to inhibit bacterial attachment by contact, as it was verified that no detectable leaching of toxic molecules occurred.
authors.affiliation: LUNAM Université, Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans, France. hope.badawy@gmail.com.
authors.forename: Hope
authors.initials: H
authors.lastname: Badawy
authors.affiliation: Centre de Transfert de Technologie du Mans, 20 rue Thalès de Milet, 72000 Le Mans, France. jbrunelliere@cttm-lemans.com.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Brunellière
authors.affiliation: LUNAM Université, Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans, France. mveryaskina@mail.ru.
authors.forename: Marina
authors.initials: M
authors.lastname: Veryaskina
authors.affiliation: LUNAM Université, Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans, France. guillaume.brotons@univ-lemans.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Brotons
authors.affiliation: LIENSs, UMR CNRS 7266, Université de La Rochelle, Bât. Marie Curie, Av. Michel Crépeau, 17042 La Rochelle, France. ssable@univ-lr.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Sablé
authors.affiliation: LIENSs, UMR CNRS 7266, Université de La Rochelle, Bât. Marie Curie, Av. Michel Crépeau, 17042 La Rochelle, France. isabelle.lanneluc@univ-lr.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lanneluc
authors.affiliation: Centre de Transfert de Technologie du Mans, 20 rue Thalès de Milet, 72000 Le Mans, France. klambert@cttm-lemans.com.
authors.forename: Kelly
authors.initials: K
authors.lastname: Lambert
authors.affiliation: Centre de Transfert de Technologie du Mans, 20 rue Thalès de Milet, 72000 Le Mans, France. pmarmey@cttm-lemans.com.
authors.forename: Pascal
authors.initials: P
authors.lastname: Marmey
authors.affiliation: Biology Department, University of Akron, Akron, OH 44325, USA. milsted@uakron.edu.
authors.forename: Amy
authors.initials: A
authors.lastname: Milsted
authors.affiliation: Department of Civil Engineering, University of Akron, Akron, OH 44325, USA. tcutrig@uakron.edu.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cutright
authors.affiliation: LUNAM Université, Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans, France. arnaud.nourry@univ-lemans.fr.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Nourry
authors.affiliation: MMS-Mer, Molécules-Santé, FR CNRS 3473 IUML, Université du Maine, 72085 Le Mans, France. Jean-Luc.Mouget@univ-lemans.fr.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Mouget
authors.affiliation: LUNAM Université, Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans, France. Pamela.Pasetto@univ-lemans.fr.
authors.forename: Pamela
authors.initials: P
authors.lastname: Pasetto
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002070:Butadienes / Q000737:chemistry / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000502:physiology; D045782:Hemiterpenes / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D010420:Pentanes / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D012623:Seawater / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000890:Anti-Infective Agents; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D011108:Polymers; C005059:isoprene