title: Nanoengineered surfaces for focal adhesion guidance trigger mesenchymal stem cell self-organization and tenogenesis.
abstract: The initial conditions for morphogenesis trigger a cascade of events that ultimately dictate structure and functions of tissues and organs. Here we report that surface nanopatterning can control the initial assembly of focal adhesions, hence guiding human mesenchymal stem cells (hMSCs) through the process of self-organization and differentiation. This process self-sustains, leading to the development of macroscopic tissues with molecular profiles and microarchitecture reminiscent of embryonic tendons. Therefore, material surfaces can be in principle engineered to set off the hMSC program toward tissuegenesis in a deterministic manner by providing adequate sets of initial environmental conditions.
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia , Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Iannone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ventre
authors.forename: Lucia
authors.initials: L
authors.lastname: Formisano
authors.forename: Laura
authors.initials: L
authors.lastname: Casalino
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Patriarca
authors.forename: Paolo A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D022001:Focal Adhesions / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D013710:Tendons / Q000166:cytology / Q000254:growth & development*; D023822:Tissue Engineering / Q000379:methods*
keywords: Stem cell; focal adhesions; nanopattern; self-organization; tenogenic differentiation; tissue development