title: Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits.
abstract: OBJECTIVE
  To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits.
  
  
  METHODS
  Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed.
  
  
  RESULTS
  No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group.
  
  
  CONCLUSIONS
  Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
authors.affiliation: Department of Ophthalmology, Santa Casa de São Paulo, São Paulo, SP, Brazil.
authors.forename: José Ricardo de Abreu
authors.initials: JR
authors.lastname: Reggi
authors.affiliation: Department of Ophthalmology, Santa Casa de São Paulo, São Paulo, SP, Brazil.
authors.forename: Richard Yudi
authors.initials: RY
authors.lastname: Hida
authors.affiliation: Department of Ophthalmology, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, SP, Brazil.
authors.forename: Milton Massato
authors.initials: MM
authors.lastname: Hida
authors.affiliation: Department of Ophthalmology, Santa Casa de São Paulo, São Paulo, SP, Brazil.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Nishiwaki-Dantas
authors.affiliation: Department of Ophthalmology, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000867:Anterior Chamber / Q000737:chemistry*; D001082:Aqueous Humor / Q000737:chemistry*; D004311:Double-Blind Method; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D056965:Injections, Intraocular / Q000379:methods; D007841:Latex Fixation Tests; D008297:Male; D023421:Models, Animal; D019152:Paracentesis; D011446:Prospective Studies; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator