title: The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression.
abstract: Diabetic nephropathy (DN) is a serious complication and it leads to kidney failure. The endothelial nitric oxide synthases (eNOS) seems to be involved in the development and progression of DN. The Puerarin is a well-known Chinese traditional formula, which is widely used in clinical practice for the treatment of kidney disease. The present study was designed to investigate the renal protective effects of Puerarin on streptozotocin (STZ)-induced diabetic rats. Thirty Sprague-Dawley (SD) male rats were divided into three groups at random. The diabetic group and the Puerarin-treated group were intraperitoneally injected with STZ 65 mg/kg and the Puerarin-treated rats were intraperitoneally injected Puerarin 100 mg/kg/day for 4 weeks. The results showed the Puerarin could improve body weight, blood sugar, BUN and SCr levels, and reduce ultrastructural changes of kidney in diabetic rats. It also attenuated eNOS expression in glomerular endothelial cells and tubular cells of diabetic rats with Puerarin treatment (p < 0.05). The Puerarin had significant renal-protective effects for the diabetic nephropathy, possibly through regulating eNOS expression, and it may be used as a potential therapeutic reagent.
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongya
authors.initials: H
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Jimin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology / Q000517:prevention & control*; D003928:Diabetic Nephropathies / Q000517:prevention & control; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000008:administration & dosage
chemical_list: D007529:Isoflavones; D013311:Streptozocin; D052250:Nitric Oxide Synthase Type III; C033607:puerarin
keywords: Diabetic nephropathy; Puerarin; eNOS; renal function; ultrastructural changes