title: Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina.
abstract: In this study, the radioprotective effect of neutral polysaccharides from Hohenbuehelia serotina (NTHSP) against the damages induced by (60)Co-γ radiation was investigated. The results showed that NTHSP could significantly improve the activity of glutathione peroxidase (GSH-Px) and increase the contents of glutathione (GSH) and ceruloplasmin in plasma after treated with 6 Gy-radiation compared with the radiation controls (p < 0.05). Furthermore, administration with NTHSP could effectively increase the quantity of marrow DNA (p < 0.05) and reduce the rates of chromosome aberration and micronuclei (p < 0.01) in bone marrows of mice. In addition, NTHSP could markedly inhibit the expressions of Bax protein and promote the expressions of Bcl-2 protein, accordingly inhibit the releases of cytochrome c and expressions of activated Caspase-3, and therefore block the mitochondrial apoptotic pathway of splenocytes in mice induced by (60)Co-γ radiation. These results suggested that NTHSP might be a natural radioprotective agent against the injuries induced by radiation.
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. Electronic address: lixiaoyu@ysu.edu.cn.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D045304:Cytochromes c / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D011837:Radiation-Protective Agents; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D004247:DNA; D005979:Glutathione Peroxidase; D002570:Ceruloplasmin; D053148:Caspase 3; D005978:Glutathione
keywords: Hohenbuehelia serotina; Isolation; Neutral polysaccharides; Radioprotective activity