title: LvDJ-1 plays an important role in resistance against Vibrio alginolyticus in Litopenaeus vannamei.
abstract: DJ-1 was first identified as an oncogene that transformed mouse NIH3T3 cells in cooperation with activated Ras. It has since exhibited a variety of functions in a range of organisms. In this study, the DJ-1 gene in Litopenaeus vannamei (LvDJ-1) was identified and characterized. A recombinant protein LvDJ-1 was produced in Pichia pastoris. LvDJ-1 expression in vivo was knocked down by dsRNA-mediated RNA interference (RNAi), which led to significantly decreased levels of LvDJ-1 mRNA and protein. When the L. vannamei were challenged with RNAi and Vibrio alginolyticus, the transcription and expression of copper zinc superoxide dismutase (LvCZSOD) in the hepatopancreas were dramatically lower in shrimp with knocked down LvDJ-1 than in controls. Transcription and expression of P53 (LvP53) were significantly higher in shrimp lacking LvDJ-1 than in controls. Hepatopancreas samples were analyzed using real time polymerase chain reaction and Western blot. Moreover, blood samples from the shrimp, assessed with flow cytometry, showed significant increases in respiratory burst and apoptosis in those lacking LvDJ-1 compared to the controls. Cumulative mortality in the shrimp lacking LvDJ-1 was significantly different from that in the control group after challenge with V. alginolyticus. Altogether, the results prove that LvDJ-1 regulates apoptosis and antioxidant activity, and that these functions play an important role in L. vannamei resistance against V. alginolyticus.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Huang
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Chenying
authors.initials: C
authors.lastname: Xie
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China. Electronic address: weina63@aliyun.com.cn.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002452:Cell Count; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics*; D006434:Hemocytes / Q000166:cytology; D043143:Hepatopancreas / Q000378:metabolism; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000382:microbiology* / Q000502:physiology; D034622:RNA Interference; D016897:Respiratory Burst; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D044149:Vibrio alginolyticus / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D015513:Oncogene Proteins; D013482:Superoxide Dismutase
keywords: Antioxidant; Apoptosis; Litopenaeus vannamei; LvDJ-1; Vibrio alginolyticus