title: Modulation of spinal glial reactivity by intrathecal PPF is not sufficient to inhibit mechanical allodynia induced by nerve crush.
abstract: Spinal glial reactivity has been strongly implicated in pain that follows peripheral nerve injury. Among the many therapeutic agents that have been tested for anti-allodynia through immune modulation is the atypical methylxanthine propentofylline. While propentofylline shows a potent anti-allodynia effect after nerve transection injury, we here demonstrate that, when propentofylline is used intrathecally at the effective immune-modulatory dose, allodynia after rat nerve crush injury is completely preserved. Microglial/macrophage Iba-1 and astrocytic GFAP expression, increased in the dorsal horn of nerve crushed animals, was, however, effectively attenuated by propentofylline. Effective modulation of spinal glial reactivity is, thus, no assurance for anti-allodynia.
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gallo
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Andrea
authors.initials: A
authors.lastname: Dimiziani
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Damblon
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Benoît
authors.initials: B
authors.lastname: Michot
authors.affiliation: Louvain Drug Research Institute, Pharmaceutics and Drug Delivery Unit, Avenue E. Mounier 73, 1200 Brussels, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Des Rieux
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: De Kock
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Hermans
authors.affiliation: Institute of Neuroscience, Université catholique de Louvain, Avenue Hippocrate B1.54.10, 1200 Brussels, Belgium. Electronic address: ronald.deumens@uclouvain.be.
authors.forename: Ronald
authors.initials: R
authors.lastname: Deumens
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006930:Hyperalgesia / Q000188:drug therapy / Q000378:metabolism* / Q000523:psychology*; D007278:Injections, Spinal; D008840:Microfilament Proteins / Q000378:metabolism; D009409:Nerve Crush; D009437:Neuralgia / Q000188:drug therapy / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism*; D017288:Pain Threshold / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D012584:Sciatic Nerve / Q000293:injuries; D066148:Spinal Cord Dorsal Horn / Q000187:drug effects / Q000378:metabolism*; D014970:Xanthines / Q000008:administration & dosage*
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D014970:Xanthines; C032114:propentofylline
keywords: Inflammation; Motor function; Nerve injury; Pain; Spared nerve injury; Treatment