title: Effects of intermittent administration of parathyroid hormone on bone augmentation in rat calvarium.
abstract: OBJECTIVE
  This study examined the effects of parathyroid hormone (PTH) on bone augmentation beyond the skeletal envelope within a plastic cap in rat calvaria.
  
  
  METHODS
  The calvaria of 30 rats were exposed, and 2 plastic caps were placed on each. Each of the 10 rats was treated with 35 or 105 μg/kg (PTH-35, PTH-105) PTH 3 times per week. The control group was injected with sterile saline 3 times per week. Micro-computed tomography (CT) imaging was performed every 2 weeks for 12 weeks. Micro-CT and histological sections were used to determine the amount of bone augmentation within the plastic caps. Bone volume (BV) was calculated using BV-measuring software.
  
  
  RESULTS
  The histomorphometric and histological analyses showed that the amount of bone augmentation was increased significantly in the PTH groups compared with the controls at 12 weeks. The PTH-105 group showed significantly more bone augmentation and osteoblasts compared with the PTH-35 group.
  
  
  CONCLUSIONS
  These results indicate that the higher the dose of intermittent PTH administered, the greater the amount of bone formation beyond the skeletal envelop in the rat calvarium.
authors.affiliation: *Graduate Student, Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan. †Associate Professor, Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan; Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan. ‡Faculty, Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan. §Professor, Nihon University School of Dentistry, Tokyo, Japan. ‖Professor and Chair, Departments of Periodontology and Operative Dentistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Biomaterials Science, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Tsunori
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.forename: Akira
authors.initials: A
authors.lastname: Hasuike
authors.forename: Soichiro
authors.initials: S
authors.lastname: Manaka
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Shino
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Sato
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kubota
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Arai
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.forename: Masashi
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D048091:Guided Tissue Regeneration / Q000379:methods; D008297:Male; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D012886:Skull / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000637:transplantation*; D055114:X-Ray Microtomography
chemical_list: D010281:Parathyroid Hormone