title: Conformational dynamics of nucleic acid molecules studied by PELDOR spectroscopy with rigid spin labels.
abstract: Nucleic acid molecules can adopt a variety of structures and exhibit a large degree of conformational flexibility to fulfill their various functions in cells. Here we describe the use of Pulsed Electron-Electron Double Resonance (PELDOR or DEER) to investigate nucleic acid molecules where two cytosine analogs have been incorporated as spin probes. Because these new types of spin labels are rigid and incorporated into double stranded DNA and RNA molecules, there is no additional flexibility of the spin label itself present. Therefore the magnetic dipole-dipole interaction between both spin labels encodes for the distance as well as for the mutual orientation between the spin labels. All of this information can be extracted by multi-frequency/multi-field PELDOR experiments, which gives very precise and valuable information about the structure and conformational flexibility of the nucleic acid molecules. We describe in detail our procedure to obtain the conformational ensembles and show the accuracy and limitations with test examples and application to double-stranded DNA.
authors.affiliation: Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Germany. Electronic address: prisner@chemie.uni-frankfurt.de.
authors.forename: T F
authors.initials: TF
authors.lastname: Prisner
authors.affiliation: Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Marko
authors.affiliation: Science Institute, University of Iceland, Reykjavik, Iceland.
authors.forename: S Th
authors.initials: ST
authors.lastname: Sigurdsson
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013113:Spin Labels
chemical_list: D013113:Spin Labels; D004247:DNA