title: Chemical profiling and quantification of Gua-Lou-Gui-Zhi decoction by high performance liquid chromatography/quadrupole-time-of-flight mass spectrometry and ultra-performance liquid chromatography/triple quadrupole mass spectrometry.
abstract: Gua-Lou-Gui-Zhi decoction (GLGZD) is a classical formula of traditional Chinese medicine, which has been commonly used to treat dysfunction after stroke, epilepsy and spinal cord injury. In this study, a systematic method was established for chemical profiling and quantification analysis of the major constituents in GLGZD. For qualitative analysis, a method of high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (Q-TOF MS) was developed. 106 compounds, including monoterpene glycosides, galloyl glucoses, phenolic acids, flavonoids, gingerols and triterpene saponins were identified or tentatively presumed by comparison with reference standards or literature data. According to the qualitative results, a new quantitative analysis method of ultra-performance liquid chromatography/triple quadrupole mass spectrometry (QqQ-MS) was established. 24 representative compounds were simultaneously detected in 10 batches of GLGZD samples in 7.5 min. The calibration curves for all analytes showed good linearity (r>0.9959) within the test ranges. The LODs and the LOQs were less than 30.6 and 70.9 ng/mL, respectively. The RSDs of intra- and inter-day precision, repeatability and stability were below 3.64%, 4.85%, 4.84% and 3.87%, respectively. The overall recoveries ranged from 94.94% to 103.66%, with the RSDs within 5.12%. This study established a high sensitive and efficient method for the integrating quality control, including identification and quantification of Chinese medicinal preparation.
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Centre of Biomedical Research&Development, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Centre of Biomedical Research&Development, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Centre of Biomedical Research&Development, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. Electronic address: yaoxuexuwei@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Centre of Biomedical Research&Development, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. Electronic address: chukd5917@163.com.
authors.forename: Kedan
authors.initials: K
authors.lastname: Chu
authors.affiliation: College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Lidian
authors.initials: L
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D016014:Linear Models; D039821:Monoterpenes / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D039821:Monoterpenes; D010636:Phenols; C584294:gua lou gui zhi
keywords: Chemical profiling and quantification; Gua-Lou-Gui-Zhi decoction; QTOF mass spectrometry; QqQ mass spectrometry; Quality control