title: New method for the determination of bile acids in human plasma by liquid-phase microextraction using liquid chromatography-ion-trap-time-of-flight mass spectrometry.
abstract: Bile acids (BAs) are derived from cholesterol and produced in the liver. The most abundant bile acids in humans are usually conjugated with glycine and taurine and are divided into primary BAs such as cholic acid (CA) and chenodeoxycholic acid (CDCA) and secondary BAs like deoxycholic acid (DCA), lithocholic acid (LCA) and ursodeoxycholic acid (UDCA). The differences amongst individual bile acids (BAs) are significant in order to distinguish different pathological processes and exposure to chemical compounds. Hollow fiber based liquid-phase microextraction (HF-LPME) is a technique that combines sample cleansing, extraction and the concentration of analytes, where a hydrophobic porous capillary membrane is impregnated with an organic extraction solvent and the lumen is filled with microliters of a phase acceptor both organic by nature. The aim of this study was to develop a new method to extract bile acids from plasma through HF-LPME of two phases (octanol as the acceptor phase) using LCMS-IT-TOF. The optimized two-phased LPME procedure for the extraction of bile acids showed limits of detection 1.0 μg L(-1) and limits of quantification of 5.0 μg L(-1). The intra-assay precision ranged from 2.1 to 11.9%. The method developed was linear over the range of 5.0-200.0 μg L(-1) for all analytes. The hollow-fiber liquid-phase microextraction method was applied to human plasma from workers exposed to organic and halogenated solvents and also to unexposed volunteers. The method is simple, low cost and it does not require large amounts of organic solvents, therefore it is quite suitable for the analysis of bile acids exposed to hepatotoxic compounds.
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Belo Horizonte/MG 31270901, Brazil; Universidade Federal de São João Del Rei - CCO, Av. Sebastião Gonçalves Coelho, 400, Divinópolis/MG 35501296, Brazil.
authors.forename: Maria José Nunes
authors.initials: MJ
authors.lastname: de Paiva
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Belo Horizonte/MG 31270901, Brazil.
authors.forename: Helvécio Costa
authors.initials: HC
authors.lastname: Menezes
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Belo Horizonte/MG 31270901, Brazil.
authors.forename: Júlio César Cardoso
authors.initials: JC
authors.lastname: da Silva
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Belo Horizonte/MG 31270901, Brazil.
authors.forename: Rodrigo Ribeiro
authors.initials: RR
authors.lastname: Resende
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Belo Horizonte/MG 31270901, Brazil. Electronic address: zenilda@ufmg.br.
authors.forename: Zenilda de Lourdes
authors.initials: Zde L
authors.lastname: Cardeal
mesh_terms: D000328:Adult; D001647:Bile Acids and Salts / Q000097:blood*; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D059627:Liquid Phase Microextraction / Q000379:methods*; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001647:Bile Acids and Salts
keywords: Bile acids; Liquid chromatography; Liquid phase microextraction; Mass spectrometry