title: Effects of 25-(OH)D3 on fecal Ca and P excretion, bone mineralization, Ca and P transporter mRNA expression and performance in growing female pigs.
abstract: A study was conducted to examine the effects of 25-hydroxyvitamin D3 (25-(OH)D3) on fecal Ca and P excretion, bone mineralization, performance and the mRNA expression of intestinal transporter genes in growing female pigs. Sixty-day old gilts (n = 24) with an average initial BW of 23.13 ± 1.49 kg were randomly allocated to a control diet (diet 1) containing wheat/corn/soybean meal and 150 IU kg(-1) of Vitamin D3, diet 1 + 50 μg of 25-(OH)D3 kg(-1) (diet 2) and diet 1 + 100 μg of 25-(OH)D3 kg(-1) (diet 3). The pigs were housed in an individual pen and had ad libitum access to feed and water for 42 days, and BWG and feed intake were measured weekly. Measures of bone mineralization and expression of Ca and P transporters mRNA were analyzed using Dual Energy X-Ray Absortiometry (DEXA) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Data were analyzed using GLM procedure of the Statistical Analysis System (SAS Institute version 9.2). Fecal Ca and P concentration were significantly reduced (P ≤ 0.05) in pigs fed diets 2 and 3 compared with the control diet. Supplementation of 25-(OH)D3 did not significantly improve bone mineralization, animal performance and intestinal transporters mRNA expression except for SLC34A1, a sodium-dependent phosphate transporter 1. In conclusion, supplementation of 25-(OH)D3 in swine nutrition may not improve animal performance but has the potential to reduce environmental pollution by increasing dietary Ca and P retention while reducing their excretion.
authors.affiliation: a Department of Animal Science , University of Manitoba , Winnipeg , Manitoba , Canada.
authors.forename: Alemu
authors.initials: A
authors.lastname: Regassa
authors.forename: Roshan
authors.initials: R
authors.lastname: Adhikari
authors.forename: Charles M
authors.initials: CM
authors.lastname: Nyachoti
authors.forename: Woo Kyun
authors.initials: WK
authors.lastname: Kim
mesh_terms: D015502:Absorptiometry, Photon / Q000662:veterinary; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002112:Calcifediol / Q000008:administration & dosage / Q000378:metabolism*; D002113:Calcification, Physiologic / Q000502:physiology; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D002136:Calcium, Dietary / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D034421:Sus scrofa / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D002136:Calcium, Dietary; D028061:Phosphate Transport Proteins; D016226:Phosphorus, Dietary; D012333:RNA, Messenger; D002112:Calcifediol
keywords: 25-(OH)D3; Fecal mineral excretion; bone mineralization; gilts; intestinal transporters