title: Lunasin inhibits cell proliferation via apoptosis and reduces the production of proinflammatory cytokines in cultured rheumatoid arthritis synovial fibroblasts.
abstract: Lunasin, a peptide with 43 amino acid residues and initially isolated and identified in soybean cotyledon, has gained extensive attention due to its anti-inflammatory and anticancer properties. However, its treatment efficacy on rheumatoid arthritis (RA) and corresponding mechanisms have not been reported. Herein, the synovial fibroblasts harvested and isolated from patients with RA were treated with lunasin at various concentrations to examine the proliferation, apoptosis status, and corresponding cell cycle of cultured RA synovial fibroblasts. Meanwhile, the underlying mechanisms of lunasin for RA treatment are explored through Western blot, real-time PCR, ELISA, and luciferase reporter assays. Lunasin significantly inhibited the proliferation and induced the apoptosis of cultured RA synovial fibroblasts. In addition, lunasin reduced the production of interleukin-6 (IL-6), IL-8, and matrix metalloproteinase-3 (MMP-3) and suppressed the activation of NF-κB in cultured RA synovial fibroblasts but did not reveal obvious modulation on the secretion and gene expression of MMP-1. Therefore, lunasin will have promising potential as a novel nutritional supplement or drug candidate for RA due to its potency of suppressing synovial cell proliferation and decreasing the production of proinflammatory cytokines and MMPs in synovial cells.
authors.affiliation: College of Health Science, Hubei Provincial Collaborative Innovation Center for Exercise and Health Promotion, Wuhan Sports University, Wuhan 430079, China.
authors.forename: Shaohui
authors.identifier: 0000-0003-4183-1399
authors.initials: S
authors.lastname: Jia
authors.affiliation: Graduate School, Wuhan Sports University, Wuhan 430079, China ; College of Sports Science and Technology, Wuhan Sports University, Wuhan 430205, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Graduate School, Wuhan Sports University, Wuhan 430079, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.affiliation: College of Health Science, Hubei Provincial Collaborative Innovation Center for Exercise and Health Promotion, Wuhan Sports University, Wuhan 430079, China.
authors.forename: Ning
authors.identifier: 0000-0002-8191-6744
authors.initials: N
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D030262:Soybean Proteins / Q000494:pharmacology*; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
chemical_list: C508606:CXCL8 protein, human; C120514:GM2S-1 protein, soybean; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D030262:Soybean Proteins; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3