title: Interleukin-8 transcripts in mononuclear cells determine impaired graft function after kidney transplantation.
abstract: OBJECTIVE
  Interleukin-8 (IL-8) has been associated with ischemia reperfusion injury after renal allograft transplantation. Impaired allograft function may cause major impact on patient morbidity and health care costs. We investigated whether transcript levels in mononuclear cells including IL-8 on the first postoperative day may be involved in immediate allograft dysfunction as defined by reduced relative change in plasma creatinine at the first postoperative day.
  
  
  METHODS
  We performed a single center, prospective-cohort study of 113 patients receiving kidney transplants. Peripheral blood mononuclear cells were harvested within 24 hours after transplantation. Transcripts were measured using quantitative RT-PCR.
  
  
  RESULTS
  Transcript levels of IL-8 and S100A8 were significantly lower in patients with relative change in plasma creatinine less than 10% at the first postoperative day. Receiver-operator characteristic curves showed that IL-8 predicted the relative change in plasma creatinine less than 10% (area under curve (AUC), 0.80; P = 0.0007). Multivariate analyses showed that lower IL-8 transcripts, longer time on dialysis, higher recipient body mass index and deceased donor type were associated with relative change in plasma creatinine at the first postoperative day less than 10%.
  
  
  CONCLUSIONS
  Reduced levels of IL-8 transcripts in peripheral mononuclear cells predict immediate graft dysfunction and delayed graft function.
authors.affiliation: Department of Nephrology, Odense University Hospital, Odense, Denmark; University of Southern Denmark, Institute of Molecular Medicine, Cardiovascular and Renal Research, Institute of Clinical Research, Odense, Denmark.
authors.forename: Christoffer
authors.initials: C
authors.lastname: Borst
authors.affiliation: Department of Nephrology, Odense University Hospital, Odense, Denmark; University of Southern Denmark, Institute of Molecular Medicine, Cardiovascular and Renal Research, Institute of Clinical Research, Odense, Denmark.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Nephrology, Odense University Hospital, Odense, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Bistrup
authors.affiliation: Department of Nephrology, Odense University Hospital, Odense, Denmark; University of Southern Denmark, Institute of Molecular Medicine, Cardiovascular and Renal Research, Institute of Clinical Research, Odense, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Tepel
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D015992:Body Mass Index; D040501:Calgranulin A / Q000235:genetics / Q000378:metabolism; D015331:Cohort Studies; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000628:therapy*; D016030:Kidney Transplantation; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011446:Prospective Studies; D012372:ROC Curve; D006435:Renal Dialysis; D013997:Time Factors
chemical_list: C508606:CXCL8 protein, human; D040501:Calgranulin A; D016209:Interleukin-8; D003404:Creatinine