title: Detection and identification of platelet-associated alloantibodies by a solid-phase modified antigen capture enzyme-linked immunosorbent assay method and its correlation to platelet refractoriness in multiplatelet concentrate-transfused patients.
abstract: Platelets express a variety of polymorphic glycoproteins (GPs), such as GPIIb/IIIa, GPib/IX, GPla/Ila, GPIV, and class I human leukocyte antigen. In the platelet transfusion setting, alloimmunization involves the production of antibodies against these glycoproteins. Patients transfused with multiple units of platelet concentrates for longer periods are the main individuals with platelet alloimmunization. This study was performed to detect the development of platelet antibodies in patients who are transfused with multiple units of leukodepleted platelet concentrates, such as those with hemato-oncologic diseases and bone marrow failure syndromes. The method used was solid phase modified antigen capture enzyme-linked immunosorbent assay. Platelet refractoriness was assessed by measuring the corrected count increment at 1 and 24 hours after transfusion.
authors.affiliation: MD (corresponding author), Transfusion Medicine, Tata Medical Centre, Kolkata, West Bengal-700156, India.
authors.forename: Neelesh
authors.initials: N
authors.lastname: Jain
authors.affiliation: MD.
authors.forename: Shankar
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: MD, Department of Immunohaematology and Blood Transfusion, Armed Forces Medical College, Pune, India-411040.
authors.forename: Joseph
authors.initials: J
authors.lastname: Philip
mesh_terms: D016824:Antigens, Human Platelet / Q000097:blood / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood / Q000276:immunology / Q000628:therapy; D006801:Humans; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D008297:Male; D017713:Platelet Transfusion; D013997:Time Factors
chemical_list: D016824:Antigens, Human Platelet; D007518:Isoantibodies