title: Hair-growth-promoting effect of conditioned medium of high integrin α6 and low CD 71 (α6bri/CD71dim) positive keratinocyte cells.
abstract: Keratinocyte stem/progenitor cells (KSCs) reside in the bulge region of the hair follicles and may be involved in hair growth. Hair follicle dermal papilla cells (HFDPCs) and outer root sheath (ORS) cells were treated with conditioned medium (CM) of KSCs. Moreover, the effects of KSC-CM on hair growth were examined ex vivo and in vivo. A human growth factor chip array and RT-PCR were employed to identify enriched proteins in KSC-CM as compared with CM from keratinocytes. KSC-CM significantly increased the proliferation of HFDPCs and ORS cells, and increased the S-phase of the cell cycle in HFDPCs. KSC-CM led to the phosphorylation of ATK and ERK1/2 in both cell types. After subcutaneous injection of KSC-CM in C3H/HeN mice, a significant increase in hair growth and increased proliferation of hair matrix keratinocytes ex vivo was observed. We identified six proteins enriched in KSC-CM (amphiregulin, insulin-like growth factor binding protein-2, insulin-like growth factor binding protein-5, granulocyte macrophage-colony stimulating factor, Platelet-derived growth factor-AA, and vascular endothelial growth factor). A growth-factor cocktail that contains these six recombinant growth factors significantly increased the proliferation of HFDPCs and ORS cells and enhanced the hair growth of mouse models. These results collectively indicate that KSC-CM has the potential to increase hair growth via the proliferative capacity of HFDPCs and ORS cells.
authors.affiliation: Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. chwon98@chol.com.
authors.forename: Chong Hyun
authors.initials: CH
authors.lastname: Won
authors.affiliation: Department of Applied Bioscience, CHA University, Seoul 135-081, Korea. iteps951@gmail.com.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Jeong
authors.affiliation: Department of Applied Bioscience, CHA University, Seoul 135-081, Korea. sjkang@cha.ac.kr.
authors.forename: Sangjin
authors.initials: S
authors.lastname: Kang
authors.affiliation: Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 305-764, Korea. kootae@cnu.ac.kr.
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Koo
authors.affiliation: Coway Cosmetics R&D Center, Seoul 153-792, Korea. shpark@coway.co.kr.
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Asan Institute for Life Sciences, Seoul 138-736, Korea. piaoid05@naver.com.
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Park
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea. ysung@knu.ac.kr.
authors.forename: Young-Kwan
authors.initials: YK
authors.lastname: Sung
authors.affiliation: College of Pharmacy, Yonsei University, Incheon 406-840, Korea. brian99@empas.com.
authors.forename: Jong-Hyuk
authors.initials: JH
authors.lastname: Sung
mesh_terms: D066258:Amphiregulin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics / Q000378:metabolism; D039503:Integrin alpha6 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism*; D008809:Mice, Inbred C3H; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D066258:Amphiregulin; D015703:Antigens, CD; C068126:CD71 antigen; D017077:Culture Media, Conditioned; D018971:Insulin-Like Growth Factor Binding Protein 2; D018975:Insulin-Like Growth Factor Binding Protein 5; D039503:Integrin alpha6; D010982:Platelet-Derived Growth Factor; D011990:Receptors, Transferrin; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; C075278:platelet-derived growth factor A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor