title: Simultaneous Determination of Trantinterol and One of Its Major Metabolites, 1-Carbonyl Trantinterol, in Human Plasma by LC-MS-MS.
abstract: A highly selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of trantinterol and one of its major metabolites, 1-carbonyl trantinterol, in human plasma. An Oasis MCX 96-well solid-phase extraction cartridge and a SeQuantTM ZIC(®)-HILIC LC column were used for sample preparation and chromatographic separation, respectively. The analytes were monitored by a QTrap 5500 mass spectrometer with positive electrospray ionization. Multiple reaction monitoring was used for quantification using the precursor to product ion pairs of m/z 311.1 → 237.9 (trantinterol), m/z 325.1 → 251.9 (1-carbonyl trantinterol) and m/z 368.4 → 294.0 (bambuterol as internal standard). The assay had a calibration range from 0.2 to 50 pg/mL and a lower limit of quantification of 0.2 pg/mL for both trantinterol and 1-carbonyl trantinterol. The inter-day and intra-day precisions were <12.0% and the accuracies were within the range of 87.1-111%. The mean recovery ranged from 82.0 to 97.7% and internal standard normalized matrix effect from 0.813 to 0.899. The analytes were stable under all tested conditions. This validated method was successfully applied to a pilot pharmacokinetic study in healthy subjects administered a single 50 μg oral dose.
authors.affiliation: Center of Clinical Pharmacology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Drug Clinical Research Centre of Shanghai Xuhui Central Hospital, Shanghai 200031, China.
authors.forename: Youli
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Center of Clinical Pharmacology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Drug Clinical Research Centre of Shanghai Xuhui Central Hospital, Shanghai 200031, China.
authors.forename: Meiwei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Center of Clinical Pharmacology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Pei
authors.affiliation: Drug Clinical Research Centre of Shanghai Xuhui Central Hospital, Shanghai 200031, China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Drug Clinical Research Centre of Shanghai Xuhui Central Hospital, Shanghai 200031, China.
authors.forename: Gangyi
authors.initials: G
authors.lastname: Liu
authors.affiliation: Center of Clinical Pharmacology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China ygp9880@126.com.
authors.forename: Guoping
authors.initials: G
authors.lastname: Yang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D002976:Clenbuterol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C542064:trantinterol; D002976:Clenbuterol