title: Histochemical and functional improvement of adipose-derived stem cell-based tissue-engineered cartilage by hyperbaric oxygen/air treatment in a rabbit articular defect model.
abstract: Cartilage is exposed to compression forces during joint loading. Therefore, exogenous stimuli are frequently used in cartilage tissue engineering strategies to enhance chondrocyte differentiation and extracellular matrix (ECM) secretion. In this study, human adipose-derived stem cells were seeded on a gelatin/polycaprolactone scaffold to evaluate the histochemical and functional improvement of tissue-engineered cartilage after hyperbaric oxygen/air treatment in a rabbit articular defect model. Behavior tests showed beneficial effects on weight-bearing and rear leg-supporting capacities after treatment of tissue-engineered cartilage with 2.5 ATA oxygen or air. Moreover, positron emission tomography images and immunohistochemistry staining demonstrated hydroxyapatite formation and increased ECM synthesis, respectively, at the tissue-engineered cartilage graft site after high pressure oxygen/air treatment. Based on these results, we concluded that hyperbaric oxygen and air treatment can improve the quality of tissue-engineered cartilage in vivo by increasing the synthesis of ECM.
authors.affiliation: From the *Division of Plastic and Reconstructive Surgery, Department of Surgery, †Burn Center, Tri-Service General Hospital, ‡Institute of Biology and Anatomy, §Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center; and ∥Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan, Republic of China.
authors.forename: Niann-Tzyy
authors.initials: NT
authors.lastname: Dai
authors.forename: Gang-Yi
authors.initials: GY
authors.lastname: Fan
authors.forename: Nien-Hsien
authors.initials: NH
authors.lastname: Liou
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Keng-Yen
authors.initials: KY
authors.lastname: Fu
authors.forename: Kuo-Hsing
authors.initials: KH
authors.lastname: Ma
authors.forename: Jiang-Chuan
authors.initials: JC
authors.lastname: Liu
authors.forename: Shun-Cheng
authors.initials: SC
authors.lastname: Chang
authors.forename: Kun-Lun
authors.initials: KL
authors.lastname: Huang
authors.forename: Lien-Guo
authors.initials: LG
authors.lastname: Dai
authors.forename: Shyi-Gen
authors.initials: SG
authors.lastname: Chen
authors.forename: Tim-Mo
authors.initials: TM
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000388:Air; D000818:Animals; D002358:Cartilage, Articular / Q000601:surgery*; D004195:Disease Models, Animal; D006651:Histocytochemistry; D006801:Humans; D006931:Hyperbaric Oxygenation; D008297:Male; D011817:Rabbits; D020127:Recovery of Function; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*