title: Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons.
abstract: Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.
authors.affiliation: Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Ioly
authors.initials: I
authors.lastname: Kotta-Loizou
authors.affiliation: Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Karakasiliotis
authors.affiliation: Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Niki
authors.initials: N
authors.lastname: Vassilaki
authors.affiliation: Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Sakellariou
authors.affiliation: Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Bartenschlager
authors.affiliation: Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece Department of Molecular Biology and Genetics, Democritus University of Thrace, Thrace, Greece penelopm@pasteur.gr.
authors.forename: Penelope
authors.initials: P
authors.lastname: Mavromara
mesh_terms: D002460:Cell Line; D015870:Gene Expression; D020869:Gene Expression Profiling; D016174:Hepacivirus / Q000235:genetics*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012093:Replicon; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D014758:Viral Core Proteins; C433031:hepatitis C protein F, Hepatitis C virus