title: Reactive DESI-MS imaging of biological tissues with dicationic ion-pairing compounds.
abstract: This work illustrates reactive desorption electrospray ionization mass spectrometry (DESI-MS) with a stable dication on biological tissues. Rat brain and zebra fish tissues were investigated with reactive DESI-MS in which the dictation forms a stable bond with biological tissue fatty acids and lipids. Tandem mass spectrometry (MS/MS) was used to characterize the dication (DC9) and to identify linked lipid-dication compounds formed. The fragment m/z 85 common to both DC9 fragmentation and DC9-lipid fragmentation was used to confirm that DC9 is indeed bonded with the lipids. Lipid signals in the range of m/z 250-350 and phosphoethanolamines (PE) m/z 700-800 observed in negative ion mode were also detected in positive ion mode with reactive DESI-MS with enhanced signal intensity. Reactive DESI-MS imaging in positive ion mode of rat brain and zebra fish tissues allowed enhanced detection of compounds commonly observed in the negative ion mode.
authors.affiliation: †Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Dragos
authors.initials: D
authors.lastname: Lostun
authors.affiliation: †Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Consuelo J
authors.initials: CJ
authors.lastname: Perez
authors.affiliation: ‡School of Chemistry, University of Nottingham, Nottingham NG7 2RD, U.K.
authors.forename: Peter
authors.initials: P
authors.lastname: Licence
authors.affiliation: §Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, U.K.
authors.forename: David A
authors.initials: DA
authors.lastname: Barrett
authors.affiliation: †Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Demian R
authors.initials: DR
authors.lastname: Ifa
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D050356:Lipid Metabolism; D057054:Molecular Imaging / Q000379:methods*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015027:Zebrafish / Q000378:metabolism