title: Effects of blocking αvβ3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats.
abstract: OBJECTIVE
  To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats.
  
  
  METHODS
  Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αvβ3 antibodies-treated and G4, anti-α2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated.
  
  
  RESULTS
  After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti- αvβ3-integrin antibodies produced similar results than DisBa-01 but not anti-α2 integrin blocking antibodies.
  
  
  CONCLUSIONS
  DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia.
authors.affiliation: Department of Medicine, Federal University of Sao Carlos, Sao Carlos, SP, Brazil.
authors.forename: Claudio Ricardo de
authors.initials: CR
authors.lastname: Oliveira
authors.affiliation: Department of Physiological Sciences, Federal University of Brasilia, Brasilia, DF, Brazil.
authors.forename: Rita de Cassia
authors.initials: Rde C
authors.lastname: Marqueti
authors.affiliation: Department of Gerontology, UFSCar, Sao Carlos, SP, Brazil.
authors.forename: Marcia Regina
authors.initials: MR
authors.lastname: Cominetti
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Physiological Sciences, UFSCar, Sao Carlos, SP, Brazil.
authors.forename: Estela Sant'Ana Vieira
authors.initials: ES
authors.lastname: Douat
authors.affiliation: Department of Chemistry, UFSCar, Sao Carlos, SP, Brazil.
authors.forename: Juliana Uema
authors.initials: JU
authors.lastname: Ribeiro
authors.affiliation: Laboratory of Cell and Molecular Biology of Cancer, Department of Physiological Sciences, College of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Carmen Lucia Salla
authors.initials: CL
authors.lastname: Pontes
authors.affiliation: Cardiopulmonary Physiotherapy Laboratory, UFSCar, Sao Carlos, SP, Brasil.
authors.forename: Audrey
authors.initials: A
authors.lastname: Borghi-Silva
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Physiological Sciences, UFSCar, Sao Carlos, SP, Brazil.
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araujo
mesh_terms: D034861:Abdominal Wall / Q000473:pathology; D000818:Animals; D003094:Collagen / Q000032:analysis / Q000187:drug effects; D004195:Disease Models, Animal; D019483:Disintegrins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006555:Hernia, Ventral / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D039302:Integrin alphaVbeta3 / Q000037:antagonists & inhibitors*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000502:physiology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D019483:Disintegrins; D039302:Integrin alphaVbeta3; D010975:Platelet Aggregation Inhibitors; D003094:Collagen; D020778:Matrix Metalloproteinase 2