title: Ion transporter NKCC1, modulator of neurogenesis in murine olfactory neurons.
abstract: Olfaction is one of the most crucial senses for vertebrates regarding foraging and social behavior. Therefore, it is of particular interest to investigate the sense of smell, its function on a molecular level, the signaling proteins involved in the process and the mechanism of required ion transport. In recent years, the precise role of the ion transporter NKCC1 in olfactory sensory neuron (OSN) chloride accumulation has been a controversial subject. NKCC1 is expressed in OSNs and is involved in chloride accumulation of dissociated neurons, but it had not been shown to play a role in mouse odorant sensation. Here, we present electro-olfactogram recordings (EOG) demonstrating that NKCC1-deficient mice exhibit significant defects in perception of a complex odorant mixture (Henkel100) in both air-phase and submerged approaches. Using next generation sequencing (NGS) and RT-PCR experiments of NKCC1-deficient and wild type mouse transcriptomes, we confirmed the absence of a highly expressed ion transporter that could compensate for NKCC1. Additional histological investigations demonstrated a reduced number of cells in the olfactory epithelium (OE), resulting in a thinner neuronal layer. Therefore, we conclude that NKCC1 is an important transporter involved in chloride ion accumulation in the olfactory epithelium, but it is also involved in OSN neurogenesis.
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and Claudia.Haering@rub.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Haering
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Ninthujah
authors.initials: N
authors.lastname: Kanageswaran
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Pascal
authors.initials: P
authors.lastname: Bouvain
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Paul
authors.initials: P
authors.lastname: Scholz
authors.affiliation: the University of Köln, Cologne Center for Genomics, Köln, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Altmüller
authors.affiliation: the University of Köln, Cologne Center for Genomics, Köln, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Becker
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Günter
authors.initials: G
authors.lastname: Gisselmann
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Janine
authors.initials: J
authors.lastname: Wäring-Bischof
authors.affiliation: From Cell Physiology, Ruhr-University Bochum, Universitaetsstr.150, 44780 Bochum, Germany and.
authors.forename: Hanns
authors.initials: H
authors.lastname: Hatt
mesh_terms: D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D015151:Immunoblotting; D017136:Ion Transport / Q000235:genetics; D008297:Male; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D055495:Neurogenesis / Q000235:genetics*; D009812:Odorants; D009831:Olfactory Mucosa / Q000378:metabolism* / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D012903:Smell; D064506:Solute Carrier Family 12, Member 2 / Q000172:deficiency / Q000235:genetics*; D059467:Transcriptome
chemical_list: D002712:Chlorides; D064506:Solute Carrier Family 12, Member 2
keywords: NKCC1; Na-K-Cl cotransporter (NKCC); Olfaction; chloride; development; electrophysiology; knockout mouse; neurobiology; neurogenesis; next generation sequencing