title: Association between tissue miR-141, miR-200c and miR-30b and bladder cancer: a matched case-control study.
abstract: OBJECTIVE
  To evaluate the expression of microRNAs in tissue samples from patients with bladder cancer and to compare it with healthy adjacent tissue samples as controls.
  
  
  METHODS
  Thirty five tissue samples from patients with newly diagnosed untreated bladder transitional cell carcinoma and 35 adjacent normal urothelium were collected during 2013 to 2014. TRIzol reagent was used to isolate total RNA including microRNAs. RNA concentration and purity were determined using a nanodrop spectrophotometer. Also 1% agarose gel electrophoresis was used to assess integrity of RNA. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) method was performed using the PARSGENOME microRNA RT-PCR system. Data was analyzed by STATA 11.
  
  
  RESULTS
  A couple of patients were female the remainder were male. Mean age of patients were 71.06 ± 11.43 years. The expression level of miR-30b, miR-141 and miR-200c in case group were significantly higher than that of control normal tissue samples. miR-141 had higher expression rate in malignant tissue than two other miRNAs (P < .001).
  
  
  CONCLUSIONS
  There was a more expression rate of miR-200c, miR-141 and miR-30b in bladder cancer tissues than healthy adjacent control tissues. Further studies are needed to draw final conclusion.
authors.forename: Ali
authors.initials: A
authors.lastname: Mahdavinezhad
authors.forename: Seyed Habibollah
authors.initials: SH
authors.lastname: Mousavibahar
authors.forename: Jalal
authors.initials: J
authors.lastname: Poorolajal
authors.forename: Reza
authors.initials: R
authors.lastname: Yadegarazari
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jafari
authors.forename: Nooshin
authors.initials: N
authors.lastname: Shabab
authors.affiliation: Department of Genetics and Molecular Medicine, Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan,Iran. sjam110@yahoo.com.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000737:chemistry* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D001743:Urinary Bladder / Q000737:chemistry*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000235:genetics
chemical_list: C533895:MIRN141 microRNA, human; C533896:MIRN200 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs