title: Effect of di-n-butyl phthalate on root physiology and rhizosphere microbial community of cucumber seedlings.
abstract: The authors investigated the effects of di-n-butyl phthalate (DBP) on root physiology and rhizosphere microbial communities of cucumber seedlings (sativus L. cv Jinyan No. 4). Root protein content and root activity were observed to decrease. From the ultrastructural micrographs, visible impact on the mitochondria, endoplasmic reticulum and vacuole were detected. Moreover, the number of starch grains increased, and some were adhered to other cell components which might be the most direct evidence of DBP causing cellular damage. Results of PCR-DGGE (denaturing gradient gel electrophoresis) indicated that DBP significantly changed the abundance, structure and composition of rhizosphere bacteria when the concentration was higher than 50 mg L(-1). The relative abundances of Firmicutes increased while that of Bacteroidetes decreased. Bacillus was detected as the dominant bacteria in DBP contaminated cucumber rhizospheric soil. The amount of Actinobacteridae and Pseudomonas decreased until it disappeared in the rhizosphere soil when exposed to DBP concentrations higher than 50 mg L(-1).
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: zhangyinghr@hotmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Tao
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Sun
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Kehinde O
authors.initials: KO
authors.lastname: Erinle
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Feng
authors.affiliation: School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Song
authors.affiliation: School of Geography, University of Nottingham, Nottinghamshire NG72RD, UK.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
mesh_terms: D018553:Cucumis sativus / Q000187:drug effects* / Q000382:microbiology*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis; D018517:Plant Roots / Q000187:drug effects* / Q000382:microbiology* / Q000648:ultrastructure; D058441:Rhizosphere; D036226:Seedlings; D012988:Soil Microbiology
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D003993:Dibutyl Phthalate
keywords: Cucumber rhizosphere microbial community; DBP; Protein content; Root activity; Ultrastructure