title: [Studies on the structure of the bioregulator purified from the rat brain].
abstract: From the brain tissue of Wistar rats,we purified a bioregulator, which is active at ultralow doses. Using reversed-phase HPLC, we prepared a homogenous polypeptide with a molecular weight of 4749 +/- 2 Da, which is responsible for the biological activity of the bioregulator. Using the CD spectroscopy method, we calculated the percentage of canonical elements of the secondary polypeptide structure in a solution. Using the methods of proteomics, we revealed that the structure of the investigated polypeptide was similar to the N-terminal sequence of a fragment of guanine-nucleotide binding G0-protein subunit alpha-1.
authors.forename: A P
authors.initials: AP
authors.lastname: Il'ina
authors.forename: A A
authors.initials: AA
authors.lastname: Moliavka
authors.forename: V P
authors.initials: VP
authors.lastname: Iamskova
authors.forename: A K
authors.initials: AK
authors.lastname: Buriak
authors.forename: I A
authors.initials: IA
authors.lastname: Iamskov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques
chemical_list: C522851:Gnai1 protein, rat; D019206:GTP-Binding Protein alpha Subunits, Gi-Go