title: The influence of leukotrienes C₄ and D₄ on the contractility of an inflamed porcine uterus.
abstract: The effect of leukotriene (LT) C4 (LTC4) and LTD4 on the contractility of an inflamed porcine uterus was investigated. On Day 3 of the estrous cycle (Day 0 of the study), either saline or Escherichia coli suspension was injected into each uterine horn. Although acute endometritis developed in all bacteria-inoculated gilts, a severe acute endometritis was noted more often on Day 8 than on Day 16. Myometrial and endometrial/myometrial strips were incubated with LTC4 or LTD4 alone, or together with a cysteinyl-LT receptor antagonist (BAY-u9773). Leukotriene C4 increased the contraction intensity in the saline- and bacteria-treated uteri on Day 8; however, its effect was lower in the myometrium of inflamed uteri. Contraction frequency was found to decrease in the saline-treated uteri as opposed to inflamed ones, in which it was elevated. On Day 16, contraction intensity increased in response to LTC4 in the saline-treated uteri but was reduced in the inflamed organs. The value of this parameter was lower in the inflamed uteri than that in the saline-treated ones. Leukotriene D4 (Days 8 and 16) augmented contractility in the saline-treated uteri, but despite increasing its intensity in the inflamed organs, it decreased contraction frequency. Leukotriene C4 or LTD4, added to BAY-u9773-pretreated saline- and bacteria-treated uteri on both days, decreased the contraction intensity. On Day 16 after treatment with BAY-u9773 and LTC4, contraction intensity in the endometrium/myometrium of the inflamed uteri was lower than that in the saline-treated organs. Data show that both LTC4 and LTD4 affect the contractility of inflamed porcine uteri, though LTC4 exerts a weaker contractile effect.
authors.affiliation: Division of Reproductive Biology, Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland. Electronic address: b.jana@pan.olsztyn.pl.
authors.forename: B
authors.initials: B
authors.lastname: Jana
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
authors.forename: J J
authors.initials: JJ
authors.lastname: Jaroszewski
authors.affiliation: Division of Reproductive Biology, Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Czarzasta
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Markiewicz
mesh_terms: D000818:Animals; D004716:Endometritis / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D004717:Endometrium / Q000503:physiopathology; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000503:physiopathology / Q000662:veterinary; D005260:Female; D017997:Leukotriene C4 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D017998:Leukotriene D4 / Q000494:pharmacology*; D009215:Myometrium / Q000503:physiopathology; D013189:SRS-A / Q000031:analogs & derivatives / Q000494:pharmacology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology*; D014590:Uterine Contraction / Q000187:drug effects*
chemical_list: C071435:BAY u9773; D013189:SRS-A; D017997:Leukotriene C4; D017998:Leukotriene D4
keywords: Gilts; Inflammation; Leukotrienes; Uterine contractility