title: CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production.
abstract: B cells are essential for antiviral immune defence because they produce neutralizing antibodies, present antigen and maintain the lymphoid architecture. Here we show that intrinsic signalling of CEACAM1 is essential for generating efficient B-cell responses. Although CEACAM1 exerts limited influence on the proliferation of B cells, expression of CEACAM1 induces survival of proliferating B cells via the BTK/Syk/NF-κB-axis. The absence of this signalling cascade in naive Ceacam1(-/-) mice limits the survival of B cells. During systemic infection with cytopathic vesicular stomatitis virus, Ceacam1(-/-) mice can barely induce neutralizing antibody responses and die early after infection. We find, therefore, that CEACAM1 is a crucial regulator of B-cell survival, influencing B-cell numbers and protective antiviral antibody responses.
authors.affiliation: Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Vishal
authors.identifier: 0000000235847530
authors.initials: V
authors.lastname: Khairnar
authors.affiliation: Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Vikas
authors.initials: V
authors.lastname: Duhan
authors.affiliation: Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Sathish Kumar
authors.initials: SK
authors.lastname: Maney
authors.affiliation: 1] Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany [2] Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Honke
authors.affiliation: 1] Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany [2] Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Namir
authors.initials: N
authors.lastname: Shaabani
authors.affiliation: Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Aleksandra A
authors.initials: AA
authors.lastname: Pandyra
authors.affiliation: Institute of Cell Biology (Cancer Research), University of Duisburg-Essen, Virchowstrasse 173, Essen 45122, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Seifert
authors.affiliation: Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Vitaly
authors.initials: V
authors.lastname: Pozdeev
authors.affiliation: 1] Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany [2] Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Haifeng C
authors.initials: HC
authors.lastname: Xu
authors.affiliation: Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Piyush
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Clinic for Primary Immunodeficiency, Medical Outpatient Unit and Immunodeficiency Laboratory, Department of Biomedicine, University Hospital, Basel 4031, Switzerland.
authors.forename: Fabian
authors.initials: F
authors.lastname: Baldin
authors.affiliation: Clinic for Primary Immunodeficiency, Medical Outpatient Unit and Immunodeficiency Laboratory, Department of Biomedicine, University Hospital, Basel 4031, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Marquardsen
authors.affiliation: 1] Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany [2] Department of Physiology I, University of Tuebingen, Gmelinstrasse 5, Tuebingen 72076, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Merches
authors.affiliation: Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lang
authors.affiliation: Institute of Medical Microbiology, Faculty of Medicine, University Hospital Essen, Hufelandstrasse 55, Essen 45122, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Kirschning
authors.affiliation: Institute of Medical Microbiology, Faculty of Medicine, University Hospital Essen, Hufelandstrasse 55, Essen 45122, Germany.
authors.forename: Astrid M
authors.initials: AM
authors.lastname: Westendorf
authors.affiliation: Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Häussinger
authors.affiliation: Department of Physiology I, University of Tuebingen, Gmelinstrasse 5, Tuebingen 72076, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.affiliation: Institute of Virology, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Dittmer
authors.affiliation: Institute of Cell Biology (Cancer Research), University of Duisburg-Essen, Virchowstrasse 173, Essen 45122, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Küppers
authors.affiliation: Clinic for Primary Immunodeficiency, Medical Outpatient Unit and Immunodeficiency Laboratory, Department of Biomedicine, University Hospital, Basel 4031, Switzerland.
authors.forename: Mike
authors.initials: M
authors.lastname: Recher
authors.affiliation: Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Hardt
authors.affiliation: Clinic of Gastroenterology and Hepatology, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Inka
authors.initials: I
authors.lastname: Scheffrahn
authors.affiliation: Rosalind and Morris Goodman Cancer Centre, Departments of Biochemistry, Medicine and Oncology, McIntyre Medical Science Building, Montreal, Quebec, Canada H3G 1Y6.
authors.forename: Nicole
authors.initials: N
authors.lastname: Beauchemin
authors.affiliation: Department of Hematology, West German Cancer Center (WTZ), University Hospital Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Joachim R
authors.initials: JR
authors.lastname: Göthert
authors.affiliation: Institute of Anatomy, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany.
authors.forename: Bernhard B
authors.initials: BB
authors.lastname: Singer
authors.affiliation: 1] Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany [2] Department of Molecular Medicine II, Heinrich Heine University Düsseldorf, Universitätsstrasse 1, Düsseldorf 40225, Germany.
authors.forename: Philipp A
authors.initials: PA
authors.lastname: Lang
authors.affiliation: 1] Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Hufelandstrasse 55, Essen 45147, Germany [2] Clinic of Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf 40225, Germany.
authors.forename: Karl S
authors.initials: KS
authors.lastname: Lang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002272:Carcinoembryonic Antigen / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010766:Phosphorylation; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism; D018116:Vesiculovirus
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D007074:Immunoglobulin G