title: Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
abstract: Medermycin and kalafungin, two antibacterial and antitumor antibiotics isolated from different streptomycetes, share an identical polyketide skeleton core. The present study reported the discovery of kalafungin in a medermycin-producing streptomycete strain for the first time. A mutant strain obtained through UV mutagenesis showed a 3-fold increase in the production of this antibiotic, compared to the wild type strain. Heterologous expression experiments suggested that its production was severely controlled by the gene cluster for medermycin biosynthesis. In all, these findings suggested that kalafungin and medermycin could be accumulated by the same streptomycete and share their biosynthetic pathway to some extent in this strain.
authors.affiliation: State Key Laboratory for Microbial Technology, Shandong University-Helmholtz Joint Institute of Biotechnology and School of Life Sciences, Shandong University, Jinan 250100, China; School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lü
authors.affiliation: School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.affiliation: School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Luyao
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cai
authors.affiliation: School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.affiliation: Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, Xinjiang, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Microbial Technology, Shandong University-Helmholtz Joint Institute of Biotechnology and School of Life Sciences, Shandong University, Jinan 250100, China; School of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Aiying
authors.initials: A
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D016227:Benzoquinones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D052978:Disk Diffusion Antimicrobial Tests; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D005810:Multigene Family; D016296:Mutagenesis / Q000528:radiation effects; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays; D000095622:Benzoisochromanequinones
chemical_list: D000900:Anti-Bacterial Agents; D016227:Benzoquinones; D009285:Naphthoquinones; C004532:quinone; C013104:medermycin; D000095622:Benzoisochromanequinones; C013079:kalafungin