title: Pulse dipolar ESR of doubly labeled mini TAR DNA and its annealing to mini TAR RNA.
abstract: Pulse dipolar electron-spin resonance in the form of double electron electron resonance was applied to strategically placed, site-specifically attached pairs of nitroxide spin labels to monitor changes in the mini TAR DNA stem-loop structure brought on by the HIV-1 nucleocapsid protein NCp7. The biophysical structural evidence was at Ångstrom-level resolution under solution conditions not amenable to crystallography or NMR. In the absence of complementary TAR RNA, double labels located in both the upper and the lower stem of mini TAR DNA showed in the presence of NCp7 a broadened distance distribution between the points of attachment, and there was evidence for several conformers. Next, when equimolar amounts of mini TAR DNA and complementary mini TAR RNA were present, NCp7 enhanced the annealing of their stem-loop structures to form duplex DNA-RNA. When duplex TAR DNA-TAR RNA formed, double labels initially located 27.5 Å apart at the 3'- and 5'-termini of the 27-base mini TAR DNA relocated to opposite ends of a 27 bp RNA-DNA duplex with 76.5 Å between labels, a distance which was consistent with the distance between the two labels in a thermally annealed 27-bp TAR DNA-TAR RNA duplex. Different sets of double labels initially located 26-27 Å apart in the mini TAR DNA upper stem, appropriately altered their interlabel distance to ~35 Å when a 27 bp TAR DNA-TAR RNA duplex formed, where the formation was caused either through NCp7-induced annealing or by thermal annealing. In summary, clear structural evidence was obtained for the fraying and destabilization brought on by NCp7 in its biochemical function as an annealing agent and for the detailed structural change from stem-loop to duplex RNA-DNA when complementary RNA was present.
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Chemical Biology and ACERT, Cornell University, Ithaca, New York.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Borbat
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Grigoryants
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York.
authors.forename: William K
authors.initials: WK
authors.lastname: Myers
authors.affiliation: Department of Chemistry and Chemical Biology and ACERT, Cornell University, Ithaca, New York.
authors.forename: Jack H
authors.initials: JH
authors.lastname: Freed
authors.affiliation: Department of Chemistry, University at Albany, State University of New York, Albany, New York. Electronic address: cps14@albany.edu.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Scholes
mesh_terms: D020029:Base Pairing; D059371:DNA, B-Form / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry; D055029:Inverted Repeat Sequences; D012367:RNA, Viral / Q000737:chemistry*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry
chemical_list: D059371:DNA, B-Form; D004279:DNA, Viral; C071884:NCP7 protein, Human immunodeficiency virus 1; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus