title: Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.
abstract: A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
authors.forename: Liba
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D002853:Chromatography, Liquid; D004355:Drug Stability; D008297:Male; D015394:Molecular Structure; D011807:Quinolizines / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines