title: Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.
abstract: A common challenge in avian cell biology is the generation of differentiated cell-lines, especially in the keratinocyte lineage. Only a few avian cell-lines are available and very few of them show an interesting differentiation profile. During the last decade, mammalian embryonic stem cell-lines were shown to differentiate into almost all lineages, including keratinocytes. Although chicken embryonic stem cells had been obtained in the 1990s, few differentiation studies toward the ectodermal lineage were reported. Consequently, we explored the differentiation of chicken embryonic stem cells toward the keratinocyte lineage by using a combination of stromal induction, ascorbic acid, BMP4 and chicken serum. During the induction period, we observed a downregulation of pluripotency markers and an upregulation of epidermal markers. Three homogenous cell populations were derived, which were morphologically similar to chicken primary keratinocytes, displaying intracellular lipid droplets in almost every pavimentous cell. These cells could be serially passaged without alteration of their morphology and showed gene and protein expression profiles of epidermal markers similar to chicken primary keratinocytes. These cells represent an alternative to the isolation of chicken primary keratinocytes, being less cumbersome to handle and reducing the number of experimental animals used for the preparation of primary cells.
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France. Electronic address: mathilde.couteaudier@hotmail.fr.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Couteaudier
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France. Electronic address: Laetitia.Fragnet@tours.inra.fr.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Trapp-Fragnet
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Auger
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Courvoisier
authors.affiliation: INRA, USC 1361, INSERM U846, Université Lyon 1, U846 S, Institut Cellules Souches et Cerveau, F-69500 Bron, France. Electronic address: bertrand.pain@inserm.fr.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Pain
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France. Electronic address: Caroline.Denesvre@tours.inra.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Denesvre
authors.affiliation: INRA, UMR 1282, Infectious Diseases and Public Health, ISP, Biova Team, Centre INRA de Tours, F-37380 Nouzilly, France. Electronic address: Jean-Francois.Vautherot@tours.inra.fr.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vautherot
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D002645:Chickens; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism