title: Bark beetle controls epithelial morphogenesis by septate junction maturation in Drosophila.
abstract: Epithelial tissues separate body compartments with different compositions. Tight junctions (TJs) in vertebrates and septate junctions (SJs) in invertebrates control the paracellular flow of molecules between these compartments. This epithelial barrier function of TJs and SJs must be stably maintained in tissue morphogenesis during cell proliferation and cell movement. Here, we show that Bark beetle (Bark), a putative transmembrane scavenger receptor-like protein, is essential for the maturation but not the establishment of SJs in Drosophila. Embryos that lack bark establish functional SJs, but due to rudimentary septae formation during subsequent embryonic development, these become non-functional. Furthermore, cell adhesion is impaired at the lateral cell membrane and the core protein complexes of SJs are mis-localised, but appear to form otherwise normally in such embryos. We propose a model in which Bark acts as a scaffold protein that mediates cell adhesion and mounting of SJ core complexes during cell rearrangement in tissue morphogenesis.
authors.affiliation: Research Group Molecular Organogenesis, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Hildebrandt
authors.affiliation: Department of Molecular Developmental Biology, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Pflanz
authors.affiliation: Developmental Biology, University of Leipzig, Talstrasse 33, D-04103 Leipzig, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Behr
authors.affiliation: Research Group Molecular Organogenesis, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Tarp
authors.affiliation: Electron Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Riedel
authors.affiliation: Research Group Molecular Organogenesis, Germany. Electronic address: rschuh@gwdg.de.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schuh
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D007365:Intercellular Junctions / Q000378:metabolism; D009154:Mutation; D051116:Receptors, Scavenger / Q000235:genetics / Q000378:metabolism*; D014132:Trachea / Q000166:cytology
chemical_list: C000598107:Bark protein, Drosophila; D029721:Drosophila Proteins; D051116:Receptors, Scavenger
keywords: Epithelial morphogenesis; Septate junction; Tracheal system