title: Synthesis of well-defined adenosine diphosphate ribose oligomers.
abstract: The post-translational modification of proteins that is known as adenosine diphosphate ribosylation (ADPr) regulates a wide variety of important biological processes, such as DNA-damage repair and cellular metabolism. This modification is also involved in carcinogenesis and the process of aging. Therefore, a better understanding of the function of ADP-ribosylation is crucial for the development of novel therapeutics. To facilitate the elucidation of the biology of ADPr, the availability of well-defined fragments of poly(ADP-ribose) is essential. Herein we report a solid-phase synthetic approach for the preparation of ADP-ribose oligomers of exactly defined length. The methodology is exemplified by the first reported synthesis of an ADP-ribose dimer and trimer.
authors.affiliation: Leiden Institute of Chemistry, Department of Bio-organic Synthesis, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands) http://biosyn.lic.leidenuniv.nl/
authors.forename: Hans A V
authors.initials: HA
authors.lastname: Kistemaker
authors.forename: Lucien N
authors.initials: LN
authors.lastname: Lameijer
authors.forename: Nico J
authors.initials: NJ
authors.lastname: Meeuwenoord
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Dmitri V
authors.initials: DV
authors.lastname: Filippov
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000737:chemistry*; D004249:DNA Damage; D004260:DNA Repair; D009682:Magnetic Resonance Spectroscopy; D010766:Phosphorylation; D011064:Poly Adenosine Diphosphate Ribose / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000246:Adenosine Diphosphate Ribose; D011064:Poly Adenosine Diphosphate Ribose
keywords: ADP-ribosylation; phosphorylation; poly(ADP-ribose); pyrophosphates; solid-phase synthesis