title: Direct interactions in the recognition between the environmental estrogen bisphenol AF and human serum albumin.
abstract: Bisphenol AF (BPAF) was used as a model compound to investigate the binding mechanism between the endocrine disrupting compound and human serum albumin (HSA) using multispectroscopic techniques and molecular modeling method at the protein level. The results indicated that BPAF was indeed bound to HSA and located in the hydrophobic pocket of HSA on subdomain IIA through hydrogen bond and van der Waals interactions. The fluorescence quenching data showed that the binding of BPAF and HSA quenched the intrinsic fluorescence of HSA, and the static quenching constants were acquired.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Junna
authors.initials: J
authors.lastname: Lv
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang, 110036, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry* / Q000633:toxicity*; D001665:Binding Sites; D052244:Endocrine Disruptors / Q000737:chemistry* / Q000633:toxicity; D004967:Estrogens / Q000737:chemistry* / Q000633:toxicity*; D006801:Humans; D006860:Hydrogen Bonding; D008958:Models, Molecular; D010636:Phenols / Q000737:chemistry* / Q000633:toxicity*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004967:Estrogens; D010636:Phenols; D012709:Serum Albumin; C583074:bisphenol AF; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
keywords: Bisphenol AF; Human serum albumin; Interaction; Multi-spectroscopic techniques