title: Comparison of human adipose-derived stem cells and chondroitinase ABC transplantation on locomotor recovery in the contusion model of spinal cord injury in rats.
abstract: OBJECTIVE
  Spinal cord injury (SCI) is one of the most serious clinical diseases and its treatment has been a subject of interest to researchers. There are two important therapeutic strategies in the treatment of SCI: replacing lost tissue cells through cells implantation and scar elimination. Therefore, in this study we used human adipose-derived stem cells (hADSCs) implantation and injection of Chondroitinase ABC. Aim of present study was to answer to this question: which one is more efficient for Improvement of locomotor recovery after SCI in rat? Transplantation of hADSCs or injection of ChABC.
  
  
  METHODS
  The spinal cord of rats was injured by contusion using a weight-drop at the level of T8-9, the hADSCs and Chondroitinase ABC were infused in to the spinal cord tissue after injury. BBB test was performed and recorded for each animal weekly for 8 weeks. After the 8(th) weeks, Serial cross-sections were stained with cresyl violet and examined under a light microscope and area of cavity in the spinal cord was measured.
  
  
  RESULTS
  At 8(th) weeks after injection, hADSCs and ChABC significantly promote locomotor function (P<0.01) and spinal cords of hADSCs and ChABC group had cavities much smaller than those of the control group (P<0.001).
  
  
  CONCLUSIONS
  Results of the present study shows dealing with inappropriate neuro-inhibitory environment and glial scar by ChABC have equal role compare to cell therapy (with hADSCs) for improving motor function after SCI and this result in adoption of proper therapeutic strategies for SCI intervention is important.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Sarveazad
authors.affiliation: Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bakhtiari
authors.affiliation: Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Asrin
authors.initials: A
authors.lastname: Babahajian
authors.affiliation: Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran ; Department of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran ; Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Atusa
authors.initials: A
authors.lastname: Janzade
authors.affiliation: Systems and Synthetic Biology Group, Bioeconomy Company, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Fallah
authors.affiliation: Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Fateme
authors.initials: F
authors.lastname: Moradi
authors.affiliation: Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran ; Cellular and Molecular Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Hematology, School of Allied Medical, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Younesi
authors.affiliation: Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Sciences, Tehran, Iran.
authors.forename: Farjam
authors.initials: F
authors.lastname: Goudarzi
authors.affiliation: Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran ; Cellular and Molecular Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.lastname: Mohammad Taghi Joghataei
keywords: BBB; Chondroitinase ABC; Contusion; Spinal Cord Injury; hADSCs