title: Early glial activation precedes neurodegeneration in the cerebral cortex after SIV infection: a 3D, multivoxel proton magnetic resonance spectroscopy study.
abstract: OBJECTIVE
  As ∼40% of HIV-infected individuals experience neurocognitive decline, we investigated whether proton magnetic resonance spectroscopic imaging ((1) H-MRSI) detects early metabolic abnormalities in the cerebral cortex of a simian immunodeficiency virus (SIV)-infected rhesus monkey model of neuroAIDS.
  
  
  METHODS
  The brains of five rhesus monkeys before and 4 or 6 weeks after SIV infection (with CD8(+) T-cell depletion) were assessed with T2 -weighted quantitative magnetic resonance imaging (MRI) and 16×16×4 multivoxel (1) H-MRSI (echo time/repetition time = 33/1440 ms). Grey matter and white matter masks were segmented from the animal MRIs and used to produce cortical masks co-registered to (1) H-MRSI data to yield cortical metabolite concentrations of the glial markers myo-inositol (mI), creatine (Cr) and choline (Cho), and of the neuronal marker N-acetylaspartate (NAA). The cortex volume within the large, 28 cm(3) (∼35% of total monkey brain) volume of interest was also calculated for each animal pre- and post-infection. Mean metabolite concentrations and cortex volumes were compared pre- and post-infection using paired sample t-tests.
  
  
  RESULTS
  The mean (± standard deviation) pre-infection concentrations of the glial markers mI, Cr and Cho were 5.8 ± 0.9, 7.2 ± 0.4 and 0.9 ± 0.1 mM, respectively; these concentrations increased 28% (p ≈ 0.06), 15% and 10% (both p < 0.05), respectively, post-infection. The mean concentration of neuronal marker NAA remained unchanged (7.0 ± 0.6 mM pre-infection vs. 7.3 ± 0.8 mM post-infection; p ≈ 0.37). The mean cortex volume was also unchanged (8.1 ± 1.1 cm(3) pre-infection vs. 8.3 ± 0.5 cm(3) post-infection; p ≈ 0.76).
  
  
  CONCLUSIONS
  These results support the hypothesis that early cortical glial activation occurs after SIV infection prior to the onset of neurodegeneration. This suggests HIV therapeutic interventions should potentially target early glial activation in the cerebral cortex.
authors.affiliation: Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: W E
authors.initials: WE
authors.lastname: Wu
authors.affiliation: Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Babb
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Tal
authors.affiliation: Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: I I
authors.initials: II
authors.lastname: Kirov
authors.affiliation: Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: George
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging and Neuroradiology Division, Massachusetts General Hospital, Charlestown, MA, USA.
authors.forename: E-M
authors.initials: EM
authors.lastname: Ratai
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging and Neuroradiology Division, Massachusetts General Hospital, Charlestown, MA, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Gonzalez
authors.affiliation: Department of Radiology, New York University School of Medicine, New York, NY, USA.
authors.forename: O
authors.initials: O
authors.lastname: Gonen
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002493:Central Nervous System Diseases / Q000209:etiology; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology*; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D008253:Macaca mulatta; D008297:Male; D066244:Proton Magnetic Resonance Spectroscopy; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications / Q000473:pathology; D015302:Simian Immunodeficiency Virus
chemical_list: D015415:Biomarkers; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline
keywords: cerebral cortex; magnetic resonance imaging; magnetic resonance spectroscopy; neuroglia; simian immunodeficiency virus