title: Measurement of microcystin -LR in water samples using improved HPLC method.
abstract: Microcystins are a group of toxic compounds produced by freshwater cyanobacteria and cause diseases. World Health Organization has recommended a concentration of 1 µg/l for Microcystin-LR (MC-LR) in potable water as guideline value. The high performance liquid chromatography (HPLC) followed by C18 analytical column and ultra violet detector for detection of MC-LR. In this regard, 5 different concentrations of MC-LR solutions were injected into HPLC. MC-LR was detected in 5.33 minute retention time and Calibration curve was achieved with R(2) = 0.988. Detection limit for this method was obtained by using acetonitrile solutions (32% and 55%) as a gradient run and a high silanol activity column equal to 0.02 µg /mL. Despite no acidic organic modifier being used in the mixture of solvents, the sensitivity of this method was appropriate for detection of MC-LR. Because of short retention time, reduction in number of solvents and high resolution and suitable sensitivity, this method is affordable and is fast for detection and determination of MC-LR in potable water.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Shamsollahi
authors.forename: Mahmood
authors.initials: M
authors.lastname: Alimohammadi
authors.forename: Ramin
authors.initials: R
authors.lastname: Nabizadeh
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Nazmara
authors.affiliation: . ahmahvi@yahoo.com.
authors.forename: Amir Hossein
authors.initials: AH
authors.lastname: Mahvi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D060766:Drinking Water / Q000032:analysis*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D060766:Drinking Water; D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C057862:cyanoginosin LR