title: Automated High-Performance Analysis of Lung Morphometry.
abstract: Automation of lung morphometric analysis is an asset in the study of lung pathophysiology because it is an assurance of robustness, reproducibility, and rapidity. The novel automated morphometric approach presented here meets these criteria. This new method collects multiple parameters, allowing quantitative elucidation of the pathophysiology of the developing and mature lungs. The automated morphometric analysis is reliable and allows the analysis of a greater proportion of each lung together with a higher number of samples and superior reproducibility than manual analysis. The use of this method revealed that treatment with 80% oxygen and lung development presented an opposite effect on most of the analyzed parameters. In conclusion, this novel approach allowed the collection of new fundamental morphometric data on lung development and a deeper comprehension of the effect of hyperoxia.
authors.affiliation: 1 Axe Reproduction, Santé de la Mère et de l'Enfant, and.|2 Centre de Recherche en Biologie de la Reproduction (CRBR) and.
authors.forename: Céline
authors.initials: C
authors.lastname: Sallon
authors.affiliation: 3 Axe Neuroscience, Centre de Recherche du CHU de Québec, Laval, Québec; and.|Départements de 4 Psychiatrie et Neuroscience and.
authors.forename: Denis
authors.initials: D
authors.lastname: Soulet
authors.affiliation: 1 Axe Reproduction, Santé de la Mère et de l'Enfant, and.|2 Centre de Recherche en Biologie de la Reproduction (CRBR) and.|5 Obstétrique/Gynécologie & Reproduction, Faculté de Médecine, Université Laval, Laval, Québec, Canada.
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Provost
authors.affiliation: 1 Axe Reproduction, Santé de la Mère et de l'Enfant, and.|2 Centre de Recherche en Biologie de la Reproduction (CRBR) and.|5 Obstétrique/Gynécologie & Reproduction, Faculté de Médecine, Université Laval, Laval, Québec, Canada.
authors.forename: Yves
authors.initials: Y
authors.lastname: Tremblay
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001997:Bronchopulmonary Dysplasia / Q000175:diagnosis*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008168:Lung / Q000473:pathology*; D051379:Mice; D012984:Software
keywords: alveolarization; bronchopulmonary dysplasia; closed area; septa; software development