title: NAF-1 antagonizes starvation-induced autophagy through AMPK signaling pathway in cardiomyocytes.
abstract: NAF-1 (nutrient-deprivation autophagy factor-1), an autophagy-related gene-related (ATG) protein, has been implicated in the autophagic pro-survival response. However, its role in autophagy has not been examined in the cardiomyocytes. In this study, we found that nutritional stress (NS) induced by glucose deprivation strongly induced autophagy in cultured neonatal rat cardiomyocytes, which was associated with NAF-1 down-regulation in cardiomyocytes under NS conditions. Furthermore, we demonstrate that ectopic expression of NAF-1 was sufficient to inhibit autophagy in cardiomyocytes under glucose deprivation conditions. Moreover, results of the co-immunoprecipitation assay indicate that NAF-1 antagonized autophagy by promoting the interaction between Beclin1 and Bcl-2 in NS-induced cardiomyocytes. Importantly, our results indicate that overexpression of NAF-1 significantly inhibited AMPK activity and protected cardiomyocytes from NS-induced cell death. Taken together, these data show that ectopic expression of NAF-1 antagonizes the degree of autophagy in cardiomyocytes and enhances cell survival during starvation conditions.
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Fuzhou
authors.initials: F
authors.lastname: Hua
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Shuchun
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Guohai
authors.initials: G
authors.lastname: Xu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D000071186:Beclin-1; D008565:Membrane Proteins / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D060066:Protein Interaction Maps; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D013217:Starvation / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; D008565:Membrane Proteins; C000605943:NAF-1 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases
keywords: Bcl-2; Beclin1; NAF-1; autophagy; starvation