title: Meta-Analysis of Gene Expression in Autism Spectrum Disorder.
abstract: Autism spectrum disorders (ASD) are clinically heterogeneous and biologically complex. In general it remains unclear, what biological factors lead to changes in the brains of autistic individuals. A considerable number of transcriptome analyses have been performed in attempts to address this question, but their findings lack a clear consensus. As a result, each of these individual studies has not led to any significant advance in understanding the autistic phenotype as a whole. Here, we report a meta-analysis of more than 1000 microarrays across twelve independent studies on expression changes in ASD compared to unaffected individuals, in both blood and brain tissues. We identified a number of known and novel genes that are consistently differentially expressed across three studies of the brain (71 samples in total). A subset of the highly ranked genes is suggestive of effects on mitochondrial function. In blood, consistent changes were more difficult to identify, despite individual studies tending to exhibit larger effects than the brain studies. Our results are the strongest evidence to date of a common transcriptome signature in the brains of individuals with ASD.
authors.affiliation: Graduate Program in Bioinformatics, University of British Columbia, Vancouver, Canada, V6T 1Z4 (C.C.).|Center for High Throughput Biology, University of British Columbia, Vancouver, Canada, V6T 1Z4 (C.C., W.K., S.R., P.P.).
authors.forename: Carolyn
authors.initials: C
authors.lastname: Ch'ng
authors.affiliation: Center for High Throughput Biology, University of British Columbia, Vancouver, Canada, V6T 1Z4 (C.C., W.K., S.R., P.P.).|Department of Psychiatry, University of British Columbia, Vancouver, Canada, V6T 1Z4 (W.K., S.R., P.P.).
authors.forename: Willie
authors.initials: W
authors.lastname: Kwok
authors.affiliation: Center for High Throughput Biology, University of British Columbia, Vancouver, Canada, V6T 1Z4 (C.C., W.K., S.R., P.P.).|Department of Psychiatry, University of British Columbia, Vancouver, Canada, V6T 1Z4 (W.K., S.R., P.P.).
authors.forename: Sanja
authors.initials: S
authors.lastname: Rogic
authors.affiliation: Center for High Throughput Biology, University of British Columbia, Vancouver, Canada, V6T 1Z4 (C.C., W.K., S.R., P.P.).|Department of Psychiatry, University of British Columbia, Vancouver, Canada, V6T 1Z4 (W.K., S.R., P.P.).
authors.forename: Paul
authors.initials: P
authors.lastname: Pavlidis
mesh_terms: D000067877:Autism Spectrum Disorder / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D046228:Microarray Analysis / Q000706:statistics & numerical data
keywords: gene expression; meta-analysis; microarray; molecular genetics