title: Alpha-glucosidase inhibitory and antiplasmodial properties of terpenoids from the leaves of Buddleja saligna Willd.
abstract: In our continuing search for biologically active natural product(s) of plant origin, Buddleja saligna, a South African medicinal plant, was screened in line with its traditional use for antidiabetic (yeast alpha glucosidase inhibitory) and antiplasmodial (against a chloroquine sensitive strain of Plasmodium falciparum (NF54)) activities. The hexane fraction showed the most promising activity with regards to its antidiabetic (IC(50) = 260 ± 0.112 µg/ml) and antiplasmodial (IC(50) = 8.5 ± 1.6 µg/ml) activities. Using activity guided fractionation three known terpenoids (betulonic acid, betulone and spinasterol) were isolated from this species for the first time. The compounds displayed varying levels of biological activities (antidiabetic: 27.31 µg/ml ≥ IC(50) ≥ 5.6 µg/ml; antiplasmodial: 14 µg/ml ≥ IC(50) ≥ 2 µg/ml) with very minimal toxicity.
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal , Pietermaritzburg , Scottsville , South Africa .
authors.forename: Jude C
authors.initials: JC
authors.lastname: Chukwujekwu
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal , Pietermaritzburg , Scottsville , South Africa .
authors.forename: Kannan R R
authors.initials: KR
authors.lastname: Rengasamy
authors.affiliation: b Department of Medicine, Division of Pharmacology , University of Cape Town , Cape Town , South Africa , and.
authors.forename: Carmen A
authors.initials: CA
authors.lastname: de Kock
authors.affiliation: b Department of Medicine, Division of Pharmacology , University of Cape Town , Cape Town , South Africa , and.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Smith
authors.affiliation: c Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic , Prague , Czech Republic.
authors.forename: Lenka Poštová
authors.initials: LP
authors.lastname: Slavětínská
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal , Pietermaritzburg , Scottsville , South Africa .
authors.forename: Johannes
authors.initials: J
authors.lastname: van Staden
mesh_terms: D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032861:Buddleja / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D021261:Parasitic Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015003:Yeasts / Q000201:enzymology; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D000962:Antimalarials; D065089:Glycoside Hydrolase Inhibitors; D013729:Terpenes; D000520:alpha-Glucosidases
keywords: Alpha-glucosidase; Buddleja saligna; antidiabetic; antiplasmodial; terpenoids