title: Novel synthesis strategy for composite hydrogel of collagen/hydroxyapatite-microsphere originating from conversion of CaCO3 templates.
abstract: Inspired by coralline-derived hydroxyapatite, we designed a methodological route to synthesize carbonated-hydroxyapatite microspheres from the conversion of CaCO3 spherulite templates within a collagen matrix under mild conditions and thus constructed the composite hydrogel of collagen/hydroxyapatite-microspheres. Fourier transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD) were employed to confirm the successful generation of the carbonated hydroxyapatite phase originating from CaCO3, and the ratios of calcium to phosphate were tracked over time. Variations in the weight portion of the components in the hybrid gels before and after the phase transformation of the CaCO3 templates were identified via thermogravimetric analysis (TGA). Scanning electron microscopy (SEM) shows these composite hydrogels have a unique multiscale microstructure consisting of a collagen nanofibril network and hydroxyapatite microspheres. The relationship between the hydroxyapatite nanocrystals and the collagen fibrils was revealed by transmission electron microscopy (TEM) in detail, and the selected area electron diffraction (SAED) pattern further confirmed the results of the XRD analyses which show the typical low crystallinity of the generated hydroxyapatite. This smart synthesis strategy achieved the simultaneous construction of microscale hydroxyapatite particles and collagen fibrillar hydrogel, and appears to provide a novel route to explore an advanced functional hydrogel materials with promising potentials for applications in bone tissue engineering and reconstruction medicine.
authors.affiliation: National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610064, People's Republic of China.
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Qiaoying
authors.initials: Q
authors.lastname: Wang
authors.forename: Hongsong
authors.initials: H
authors.lastname: Fan
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry; D002119:Calcium Carbonate / Q000737:chemistry*; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D004583:Electrons; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D053758:Nanoparticles; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen; D017886:Durapatite; D002119:Calcium Carbonate