title: Extensive Characterization of Platelet Gel Releasate From Cord Blood in Regenerative Medicine.
abstract: Platelet gel derived from peripheral blood is widely applied in many clinical fields of surgery as biomaterial containing growth factors with high proliferative properties. In 2010, we studied and patented a platelet gel derived from cord blood. In this study, due to the crucial role of the factors released by the platelet gel, we first extended the characterization of its releasate. Using a wide proteomic array and splitting the two components of the releasate, that is, platelets and plasma, we have been able to study their growth factor content. Interestingly, we discovered high levels of hormones and molecules able to support tissue growth in the cord blood platelet gel releasate and, in addition, higher concentrations of several angiogenic factors if compared with the peripheral blood counterpart. On the contrary, the latter was much richer in inflammatory factors. The second aim of our work was to study the effects on cell culture, immunophenotype, and function of mesenchymal stem cells exposed to these two platelet gel releasates as substitute for the animal serum. Since our findings nicely show that the use of the peripheral versus the cord blood platelet gel releasate can differently influence the mesenchymal stem cell commitment, we can suggest that in addition to its peculiar angiogenic properties cord blood platelet gel releasate shows excellent proliferative properties as cell culture supplement.
authors.affiliation: Cell Factory, Unit for Cellular Therapy and Cryobiology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Parazzi
authors.forename: Cristiana
authors.initials: C
authors.lastname: Lavazza
authors.forename: Valentina
authors.initials: V
authors.lastname: Boldrin
authors.forename: Elisa
authors.initials: E
authors.lastname: Montelatici
authors.forename: Francesco
authors.initials: F
authors.lastname: Pallotti
authors.forename: Maurizio
authors.initials: M
authors.lastname: Marconi
authors.forename: Lorenza
authors.initials: L
authors.lastname: Lazzari
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002457:Cell Extracts / Q000032:analysis* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology*; D020869:Gene Expression Profiling; D006801:Humans; D007231:Infant, Newborn; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D020543:Proteome / Q000032:analysis; D044968:Regenerative Medicine
chemical_list: D002457:Cell Extracts; D036341:Intercellular Signaling Peptides and Proteins; D020543:Proteome