title: Lymphangiosarcoma with bone formation of the auricle in a dog.
abstract: A 12-year-old mixed-breed neutered female dog was referred with cutaneous tumors at the left auricle. Histologically, the cutaneous tumor located in the dermis comprised numerous clefts and cavernous channels lined by neoplastic endothelial cells with no erythrocytes. Bone tissue without direct contact with neoplastic cells was seen in the well-developed stromal connective tissue. The neoplastic endothelial cells exhibited mild to moderate atypia. Immunohistochemically, neoplastic cells were positive for vimentin and negative for cytokeratin and factor VIII-related antigen. Basement membrane around the neoplastic lumens was positive for laminin in a linear or granular pattern. Ultrastructural examination revealed discontinuous basement membrane beneath the tumor cells. Histopathological features of this case were consistent with lymphangiosarcoma, and stromal ossification was characteristic.
authors.affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mineshige
authors.forename: Go
authors.initials: G
authors.lastname: Sugahara
authors.forename: Tamio
authors.initials: T
authors.lastname: Ohmuro
authors.forename: Junichi
authors.initials: J
authors.lastname: Kamiie
authors.forename: Kinji
authors.initials: K
authors.lastname: Shirota
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D004428:Ear Neoplasms / Q000473:pathology / Q000662:veterinary*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007797:Laminin / Q000378:metabolism; D008204:Lymphangiosarcoma / Q000473:pathology / Q000662:veterinary*; D009999:Ossification, Heterotopic / Q000473:pathology / Q000662:veterinary*; D014746:Vimentin / Q000378:metabolism
chemical_list: D007797:Laminin; D014746:Vimentin