title: Notch Signaling Pathway Regulates Progesterone Secretion in Murine Luteal Cells.
abstract: Notch signaling is an evolutionarily conserved pathway, which involves in various cell life activities. Other studies and our report showed that the Notch signaling plays very important role in follicle development in mammalian ovaries. In luteal cells, Notch ligand, delta-like ligand 4, is involved in normal luteal vasculature. In this study, murine luteal cells were cultured in vitro and treated with Notch signaling inhibitors, L-658,458 and N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycinet-butyl ester (DAPT). We found that L-658,458 and DAPT treatment decrease basal and human chorionic gonadotropin (hCG)-stimulated progesterone secretion. On the contrary, overexpression of intracellular domain of Notch3 increased basal and hCG-stimulated progesterone secretion. Further studies demonstrated that Notch signaling regulated the expression of steroidogenic acute regulatory protein and CYP11A, 2 key enzymes for progesterone synthesis. In conclusion, Notch signaling plays important role in regulating progesterone secretion in murine luteal cells.
authors.affiliation: Department of Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Peng
authors.affiliation: Joint Programme of Nanchang University and Queen Mary University of London, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Joint Programme of Nanchang University and Queen Mary University of London, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Riqiang
authors.initials: R
authors.lastname: Bao
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China zhangcp81@gmail.com.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004151:Dipeptides / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D006063:Chorionic Gonadotropin; D004151:Dipeptides; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; C499373:Notch3 protein, mouse; D010750:Phosphoproteins; D000071656:Receptor, Notch3; D051880:Receptors, Notch; D000098682:Steroidogenic Acute Regulatory Protein; D011374:Progesterone; D002786:Cholesterol Side-Chain Cleavage Enzyme
keywords: Notch3; luteal cell; progesterone