title: Basal cell carcinoma vs basaloid squamous cell carcinoma of the skin: an immunohistochemical reappraisal.
abstract: Typical cutaneous basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are morphologically dissimilar. It is well known, however, that poorly differentiated SCC may assume a basaloid phenotype, complicating the histologic distinction between these 2 neoplasms. Selected immunohistochemical stains have been used in the past to aid in that differential diagnosis. In the current study, additional markers were evaluated to determine whether they would be helpful in that regard. Twenty-nine cases of metatypical (squamoid) BCC (MBCC) and 25 examples of basaloid SCC (BSCC) were studied using the antibodies Ber-EP4 and MOC-31 as well as a plant lectin preparation from Ulex europaeus I (UEA-1). The resulting immunostains were interpreted independently by 3 pathologists, and the results showed that MBCCs demonstrated strong and diffuse staining for Ber-EP4 (25/29) and MOC-31 (29/29). In contrast, BSCCs tended to be only sporadically reactive for both markers (4/25 and 1/25 cases, respectively). Labeling for UEA-1 was observed in almost all BSCCs (24/25), but only 6 of 29 cases of MBCC showed limited, focal staining with that lectin. These data suggest that MOC-31 is a useful marker in the specified differential diagnosis, especially when used together with UEA-1.
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, VA.
authors.forename: David V
authors.initials: DV
authors.lastname: Webb
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, VA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Mentrikoski
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, VA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Verduin
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, VA.
authors.forename: Louis B
authors.initials: LB
authors.lastname: Brill
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, VA. Electronic address: mrw9c@virginia.edu.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wick
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism; D002280:Basal Cell Carcinoma / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D012878:Skin Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D039762:Ulex / Q000737:chemistry; D000951:Antigens, Neoplasm
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C546130:MOC-31 monoclonal antibody, human; C115012:human epithelial antigen-125; D000951:Antigens, Neoplasm
keywords: Basal cell carcinoma; Ber-EP4; MOC-31; Skin neoplasms; Squamous cell carcinoma; Ulex europaeus