title: Reproductive toxicity in male mice after exposure to high molybdenum and low copper concentrations.
abstract: To evaluate the effects of dietary high molybdenum (HMo) and low copper (LCu) concentrations on reproductive toxicity of male mice, 80 mice were divided into 4 groups of 20. These groups were fed with the following: (1) normal control (NC) diet (NC group); (2) NC and HMo diets (HMo group); (3) LCu diet (LCu group); and (4) HMo and LCu diets (HMoLCu group). On the 50th and 100th day, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) were analyzed to determine oxidative stress states. Morphological changes in testicular tissue were evaluated with hematoxylin and eosin staining and ultrastructural changes were monitored by transmission electron microscopy. The results showed that administration of HMo, LCu, and HMoLCu not only decreased sperm density and motility but also increased the rate of teratosperm occurrence. A significant increase in MDA content and a decrease in SOD, GSH-Px, and T-AOC contents were observed in LCu, HMo, and HMoLCu groups. Testicular tissues and cells of mice were damaged by HMo and the damages were more serious in the case of Cu deficiency. Exposure to HMo adversely affected the reproductive system of male mice, and dietary LCu plays key roles in HMo-induced reproductive toxicity.
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China haustzbh@163.com.
authors.forename: Bian-Hua
authors.initials: BH
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Biological Engineering, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, People's Republic of China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China.
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, People's Republic of China.
authors.forename: Er-Jie
authors.initials: EJ
authors.lastname: Tian
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D003300:Copper / Q000172:deficiency*; D003677:Deficiency Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D004032:Diet / Q000009:adverse effects*; D000075322:Heavy Metal Poisoning; D007248:Infertility, Male / Q000209:etiology*; D015227:Lipid Peroxidation; D008297:Male; D019216:Metals, Heavy / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum / Q000506:poisoning*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000097:blood / Q000378:metabolism; D011041:Poisoning / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D000072660:Teratozoospermia / Q000209:etiology; D013737:Testis / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D015430:Weight Gain
chemical_list: D019216:Metals, Heavy; D003300:Copper; D008982:Molybdenum; D010088:Oxidoreductases
keywords: Molybdenum; copper; lipid peroxidation; mice; oxidative stress; testicular tissue; ultrastructure