title: Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40.
abstract: Heat-killed Lactobacillus plantarum strain L-137 (HK L-137) is a more potent inducer of interleukin (IL)-12 than other heat-killed Lactobacillus strains. To elucidate the mechanism involved in this IL-12p40 induction, we compared HK L-137 with heat-killed L. plantarum strain JCM1149 (HK JCM1149) by nuclear magnetic resonance and mass spectrometry. Results showed that HK L-137 contained lipoteichoic acid (LTA) with a chemical structure similar to that of JCM1149, except for a lower degree of glucosyl substitution in the poly(glycerol phosphate) backbone. Lysozyme sensitivity and electrophoretic moiety analysis revealed that HK L-137 exposed more LTA on its cell surface than HK JCM1149. Phagocytosis of HK L-137 by splenic adherent cells was significantly greater than that of HK JCM1149. Anti-LTA antibody and anti-scavenger receptor-A (SR-A) antibody selectively inhibited phagocytosis of HK L-137, as well as IL-12p40 production, by splenic adherent cells. Thus, a higher efficiency of phagocytosis of HK L-137 via SR-A for LTA is responsible for the potent IL-12p40 induction.
authors.affiliation: Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hatano
authors.affiliation: Research and Development Institute, House Wellness Foods Corporation, 3-20 Imoji, Itami, Hyogo 664-0011, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Research and Development Institute, House Wellness Foods Corporation, 3-20 Imoji, Itami, Hyogo 664-0011, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Research and Development Institute, House Wellness Foods Corporation, 3-20 Imoji, Itami, Hyogo 664-0011, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Murosaki
authors.affiliation: Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan. Electronic address: yoshikai@bioreg.kyushu-u.ac.jp.
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Yoshikai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016207:Cytokines; D005260:Female; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010587:Phagocytosis / Q000187:drug effects; D017452:Receptors, IgG / Q000235:genetics; D051116:Receptors, Scavenger / Q000276:immunology / Q000378:metabolism*; D013682:Teichoic Acids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000906:Antibodies; D016207:Cytokines; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; D017452:Receptors, IgG; D051116:Receptors, Scavenger; D013682:Teichoic Acids; C009900:lipoteichoic acid
keywords: Bacteria; Immunotherapy; Interleukin-12; Phagocytosis; Scavenger receptor