title: Fractionation of Plasmodium-infected human red blood cells to study protein trafficking.
abstract: Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.
authors.affiliation: Research School of Biology, The Australian National University, Canberra, Australia.
authors.forename: Simone
authors.initials: S
authors.lastname: Külzer
authors.forename: Verena
authors.initials: V
authors.lastname: Bittl
authors.forename: Jude M
authors.initials: JM
authors.lastname: Przyborski
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D057075:Enzyme Assays; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology*; D006461:Hemolysis; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D010539:Permeability; D010961:Plasmodium / Q000502:physiology*; D021381:Protein Transport; D013301:Streptolysins / Q000378:metabolism*; D013347:Subcellular Fractions
chemical_list: D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O; D010447:Peptide Hydrolases