title: Studying coronavirus-host protein interactions.
abstract: To understand the molecular mechanisms of viral replication and pathogenesis, it is necessary to establish the virus-host protein interaction networks. The yeast two-hybrid system is a powerful proteomic approach to study protein-protein interactions. After the identification of specific cellular factors interacting with the target viral protein using the yeast two-hybrid screening system, co-immunoprecipitation and confocal microscopy analyses are often used to verify the virus-host protein interactions in cells. Identification of the cellular factors required for viral survival or eliminating virus infected cells could help scientists develop more effective antiviral drugs. Here we summarize a standard protocol used in our lab to study the coronavirus-host protein interactions, including yeast two-hybrid screening, co-immunoprecipitation, and immunofluorescence microscopy analyses.
authors.affiliation: Institute of Medical Sciences, Tzu Chi University, Hualien, 97004, Taiwan.
authors.forename: Chee-Hing
authors.initials: CH
authors.lastname: Yang
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Li
authors.forename: Cheng-Huei
authors.initials: CH
authors.lastname: Hung
authors.forename: Shih-Yen
authors.initials: SY
authors.lastname: Lo
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D017934:Coronavirus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D060066:Protein Interaction Maps; D020798:Two-Hybrid System Techniques; D014709:Vero Cells; D053585:Virus Attachment