title: The effect of photoinitiators on intracellular AKT signaling pathway in tissue engineering application.
abstract: Free-radical photopolymerization initiated by photoinitiators is an important method to make tissue engineering scaffolds. To advance understanding of photoinitiator cytocompatibility, we examined three photoinitiators including 2,2-dimethoxy-2-phenylacetophenone (DMPA), Irgacure 2959 (I-2959), and eosin Y photoinitiating system (EY) in terms of their effects on viability of HN4 cells and expression levels of intracellular AKT and its phosphorylated form p-AKT. Our results show that the photoinitiators and their UV-exposed counterparts affect intracellular AKT signaling, which can be used in conjunction with cell viability for cytocompatibility assessment of photoinitiators.
authors.affiliation: Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284.
authors.forename: Natasha
authors.initials: N
authors.lastname: Sheybani
authors.affiliation: Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298 ; Massey Cancer Center; Virginia Commonwealth University, Richmond, Virginia 23298.
authors.forename: W Andrew
authors.initials: WA
authors.lastname: Yeudall
authors.affiliation: Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284 ; Massey Cancer Center; Virginia Commonwealth University, Richmond, Virginia 23298.
authors.forename: Hu
authors.initials: H
authors.lastname: Yang
mesh_terms: D000098:Acetophenones / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D010777:Photochemistry; D058105:Polymerization; D051057:Proto-Oncogene Proteins c-akt / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000098:Acetophenones; C452198:2,2-dimethoxy-2-phenylacetophenone; D051057:Proto-Oncogene Proteins c-akt