title: The receptor-like cytoplasmic kinase PCRK1 contributes to pattern-triggered immunity against Pseudomonas syringae in Arabidopsis thaliana.
abstract: In this paper we describe PATTERN-TRIGGERED IMMUNITY (PTI) COMPROMISED RECEPTOR-LIKE CYTOPLASMIC KINASE 1 (PCRK1) of Arabidopsis thaliana, an RLCK that is important for defense against the pathogen Pseudomonas syringae pv. maculicola ES4326 (Pma ES4326). We examined defense responses such as bacterial growth, production of reactive oxygen species (ROS) and callose deposition in pcrk1 mutant plants to determine the role of PCRK1 during pathogen infection. Expression of PCRK1 was induced following pathogen infection. Pathogen growth was significantly higher in pcrk1 mutant lines than in wild-type Col-0. Mutant pcrk1 plants showed reduced pattern-triggered immunity (PTI) against Pma ES4326 after pretreatment with peptides derived from flagellin (flg22), elongation factor-Tu (elf18), or an endogenous protein (pep1). Deposition of callose was reduced in pcrk1 plants, indicating a role of PCRK1 in activation of early immune responses. A PCRK1 transgene containing a mutation in a conserved lysine residue important for phosphorylation activity of kinases (K118E) failed to complement a pcrk1 mutant for the Pma ES4326 growth phenotype. Our study shows that PCRK1 plays an important role during PTI and that a conserved lysine residue in the putative kinase domain is important for PCRK1 function.
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Suma
authors.initials: S
authors.lastname: Sreekanta
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Gerit
authors.initials: G
authors.lastname: Bethke
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hatsugai
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tsuda
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Thao
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Katagiri
authors.affiliation: Department of Plant Biology and Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, Saint Paul, MN, 55108, USA.
authors.forename: Jane
authors.initials: J
authors.lastname: Glazebrook
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D005408:Flagellin / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005936:Glucans / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism*; D057865:Plant Immunity / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; D000069452:Pathogen-Associated Molecular Pattern Molecules; D017382:Reactive Oxygen Species; D005408:Flagellin; C048306:callose; C000606408:PCRK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D008239:Lysine; D020156:Salicylic Acid
keywords: Arabidopsis; PTI COMPROMISED RECEPTOR-LIKE CYTOPLASMIC KINASE 1 (PCRK1); Pseudomonas syringae; flg22; immunity; pathogen/microbe-associated molecular pattern (PAMP/MAMP); pattern-triggered immunity (PTI); receptor-like cytoplasmic kinase