title: Analytical methods for kinetic studies of biological interactions: A review.
abstract: The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: Cong
authors.initials: C
authors.lastname: Bi
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Podariu
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588, USA. Electronic address: dhage1@unl.edu.
authors.forename: David S
authors.initials: DS
authors.lastname: Hage
mesh_terms: D000818:Animals; D001688:Biological Products / Q000032:analysis* / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods* / Q000639:trends; D019075:Electrophoresis, Capillary / Q000379:methods / Q000639:trends; D006801:Humans; D007700:Kinetics; D011485:Protein Binding / Q000502:physiology; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000639:trends
chemical_list: D001688:Biological Products
keywords: Affinity chromatography; Capillary electrophoresis; Kinetics of biological interactions; Stopped-flow analysis; Surface plasmon resonance spectroscopy