title: Citrus polyphenol for oral wound healing in oral ulcers and periodontal diseases.
abstract: OBJECTIVE
  Various polyphenolic compounds from plants have been confirmed to have different pharmaceutical functions. The purpose of this study was to evaluate citrus polyphenol (CP) for dental applications. A medium with CP was developed to improve oral wound healing. The CP could be used as a supplemental compound in mouthwash for periodontal diseases.
  
  
  METHODS
  In this study, the metabolic activity and cell toxicity of CP (1%, 0.1%, and 0.01%) for fibroblasts were investigated by MTT and lactate dehydrogenase assays (n = 6). The effect of CP on motility of fibroblast was also evaluated via a wound healing model.
  
  
  RESULTS
  The growth of Hs68 cells on TCPS was greatly increased in the presence of 0.01% CP. In addition, the signiﬁcant difference (p<0.01) of cell toxicity of fibroblast was observed after 6 days in 0.01% CP medium. Using the wound healing model, it was also found that CP could enhance the migratory ability of fibroblasts.
  
  
  CONCLUSIONS
  The results confirm the feasibility of CP be a supplemental compound in mouthwash for treatment of periodontal diseases in dental application to improve wound healing in the mouth.
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan, ROC; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Hsiao-Cheng
authors.initials: HC
authors.lastname: Tsai
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Li
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan, ROC. Electronic address: thyoung@ntu.edu.tw.
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan, ROC. Electronic address: minhueychen@ntu.edu.tw.
authors.forename: Min-Huey
authors.initials: MH
authors.lastname: Chen
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002957:Citrus / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D019226:Oral Ulcer / Q000188:drug therapy; D010510:Periodontal Diseases / Q000188:drug therapy; D059808:Polyphenols / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D059808:Polyphenols
keywords: citrus polyphenol; proliferation; wound healing