title: Probing the interaction of distamycin A with S100β: the "unexpected" ability of S100β to bind to DNA-binding ligands.
abstract: DNA-minor-groove-binding ligands are potent antineoplastic molecules. The antibiotic distamycin A is the prototype of one class of these DNA-interfering molecules that have been largely used in vitro. The affinity of distamycin A for DNA is well known, and the structural details of the complexes with some B-DNA and G-quadruplex-forming DNA sequences have been already elucidated. Here, we show that distamycin A binds S100β, a protein involved in the regulation of several cellular processes. The reported affinity of distamycin A for the calcium(II)-loaded S100β reinforces the idea that some biological activities of the DNA-minor-groove-binding ligands arise from the binding to cellular proteins.
authors.affiliation: Giotto Biotech, Via Madonna del Piano 6, Sesto Fiorentino, Florence, 50019, Italy.
authors.forename: Linda
authors.initials: L
authors.lastname: Cerofolini
authors.forename: Jussara
authors.initials: J
authors.lastname: Amato
authors.forename: Valentina
authors.initials: V
authors.lastname: Borsi
authors.forename: Bruno
authors.initials: B
authors.lastname: Pagano
authors.forename: Antonio
authors.initials: A
authors.lastname: Randazzo
authors.forename: Marco
authors.initials: M
authors.lastname: Fragai
mesh_terms: D001665:Binding Sites; D004214:Distamycins / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D064568:S100 Calcium Binding Protein beta Subunit / Q000737:chemistry*
chemical_list: D004214:Distamycins; D008024:Ligands; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human; C030000:stallimycin
keywords: NMR spectroscopy; calcium binding protein S100β; calorimetry; distamycin A; docking; fluorescence; noncovalent interactions