title: Molecular screening for Epstein Barr virus (EBV) among Sudanese patients with nasopharyngeal carcinoma (NPC).
abstract: OBJECTIVE
  The aim of this study was to screen for the presence of Epstein Barr Virus (EBV) among Sudanese patients with Nasopharyngeal Carcinoma (NPC).
  
  
  METHODS
  In this study, 150 tissue samples that were previously diagnosed as having NPC were screened for the presence of EBV using Polymerase Chain Reaction (PCR). PCR was performed to amplify two viral genes; EBV nuclear antigen-4 (EBNA-4) and latent membrane protein-1 (LMP1).
  
  
  RESULTS
  EBV genes were detected in 92/150 (61.3%) tissue samples. Of the 92 infected samples, 58/92 (63%) were found among males and 34/92 (37%) were among females.
  
  
  CONCLUSIONS
  EBV is prevalent in the Sudan and responsible of the vast majority of cases of NPC.
authors.affiliation: Department of Pathology, College of Medicine, Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, Hail, Kingdom of Saudi Arabia ; Department of Histopathology and Cytology, FMLS, University of Khartoum, Hail, Sudan.
authors.forename: Hussain Gadelkarim
authors.initials: HG
authors.lastname: Ahmed
authors.affiliation: Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Khartoum, Sudan.
authors.forename: Rania Saad Abdul Gader
authors.initials: RS
authors.lastname: Suliman
authors.affiliation: Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Khartoum, Sudan.
authors.forename: Mohammed Siddig Abd
authors.initials: MS
authors.lastname: El Aziz
authors.affiliation: Department of Medical Laboratory Science, College of Applied Medical Science, University of Hail, Hail, Kingdom of Saudi Arabia.
authors.forename: Fawaz D
authors.initials: FD
authors.lastname: Alshammari
keywords: Epstein barr virus; Nasopharyngeal carcinoma; Sudan