title: Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis.
abstract: It is well known that paraoxonase-1 (PON1) activity may decrease during the course of infection and inflammation. The aim of this study was to investigate serum PON1 activity, oxidative status, and thiols levels in patients with acute brucellosis. In addition, we investigated the PON1 phenotype in patients with acute brucellosis. Thirty patients with acute brucellosis and 35 healthy controls were enrolled. Serum paraoxonase and arylesterase activities, thiols levels, lipid hydroperoxide levels, total antioxidant capacity (TAC), total oxidant status (TOS) and oxidative stress index (OSI) were determined. Serum basal and salt-stimulated paraoxonase-arylesterase activities, TAC levels and thiols levels were significantly lower in patients with acute brucellosis than controls (for all, p < 0.05), while LOOH levels, TOS levels, and OSI values were significantly higher (for all, p < 0.05). We concluded that oxidative stress is increased, while serum PON1 activity is decreased in patients with acute brucellosis. These results indicate that lower PON1 activity is associated with oxidant-antioxidant imbalance.
authors.affiliation: Medical Faculty, Department of Internal Medicine, Yuzuncu Yıl University, 65000, Van, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Esen
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aslan
authors.forename: Mehmet Emin
authors.initials: ME
authors.lastname: Kucukoglu
authors.forename: Aytekin
authors.initials: A
authors.lastname: Cıkman
authors.forename: Umit
authors.initials: U
authors.lastname: Yakan
authors.forename: Mahmut
authors.initials: M
authors.lastname: Sunnetcioglu
authors.forename: Sahbettin
authors.initials: S
authors.lastname: Selek
mesh_terms: D000208:Acute Disease; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002006:Brucellosis / Q000097:blood*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008297:Male; D016877:Oxidants / Q000097:blood*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human