title: Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae.
abstract: We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.
authors.affiliation: MEMPHYS-Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Henrik Seir
authors.initials: HS
authors.lastname: Thoke
authors.affiliation: MEMPHYS-Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Asger
authors.initials: A
authors.lastname: Tobiesen
authors.affiliation: MEMPHYS-Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Brewer
authors.affiliation: MEMPHYS-Center for Biomembrane Physics, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Per Lyngs
authors.initials: PL
authors.lastname: Hansen
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México (IBt-UNAM), Av. Universidad 2001, Cuernavaca, Morelos, 62210, Mexico.
authors.forename: Roberto P
authors.initials: RP
authors.lastname: Stock
authors.affiliation: CelCom group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Lars F
authors.initials: LF
authors.lastname: Olsen
authors.affiliation: MEMPHYS-Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Bagatolli
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D017666:Deuterium Oxide / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes; D006019:Glycolysis; D008660:Metabolism; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D014867:Water / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D009243:NAD; D000255:Adenosine Triphosphate; D017666:Deuterium Oxide