title: L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor.
abstract: l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess "stemness" during long-term culture.
authors.affiliation: Cell Therapy and Tissue Engineering Center .
authors.forename: Sung Hae
authors.initials: SH
authors.lastname: Bae
authors.forename: Hoon
authors.initials: H
authors.lastname: Ryu
authors.forename: Ki-Jong
authors.initials: KJ
authors.lastname: Rhee
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Oh
authors.forename: Soon Koo
authors.initials: SK
authors.lastname: Baik
authors.forename: Kwang Yong
authors.initials: KY
authors.lastname: Shim
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Kong
authors.forename: Shin Young
authors.initials: SY
authors.lastname: Hyun
authors.forename: Hyun Sung
authors.initials: HS
authors.lastname: Pack
authors.forename: Changjo
authors.initials: C
authors.lastname: Im
authors.forename: Ha Cheol
authors.initials: HC
authors.lastname: Shin
authors.forename: Yong Man
authors.initials: YM
authors.lastname: Kim
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Eom
authors.forename: Jong In
authors.initials: JI
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000328:Adult; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D001343:Autophagy; D001854:Bone Marrow Cells / Q000187:drug effects*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D064368:Healthy Volunteers; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult
chemical_list: C066855:HGF protein, human; D017382:Reactive Oxygen Species; D016222:Fibroblast Growth Factor 2; C011669:ascorbate-2-phosphate; D017228:Hepatocyte Growth Factor; D001205:Ascorbic Acid
keywords: Bone marrow-derived mesenchymal stem cell; fibroblast growth factor-2; hepatocyte growth factor; l-ascorbic acid 2-phosphate; stemness