title: Ultrasensitive detection of microRNA through rolling circle amplification on a DNA tetrahedron decorated electrode.
abstract: MicroRNAs are a class of evolutionally conserved, small noncoding RNAs involved in the regulation of gene expression and affect a variety of biological processes including cellular differentiation, immunological response, tumor development, and so on. Recently, microRNAs have been identified as promising disease biomarkers. In this work, we have fabricated a novel electrochemical method for ultrasensitive detection of microRNA. Generally, a DNA tetrahedron decorated gold electrode is employed as the recognition interface. Then, hybridizations between DNA tetrahedron, microRNA, and primer probe initiate rolling circle amplification (RCA) on the electrode surface. Silver nanoparticles attached to the RCA products provide significant electrochemical signals and a limit of detection as low as 50 aM is achieved. Moreover, homology microRNA family members with only one or two mismatches can be successfully distinguished. Therefore, this proposed method reveals great advancements toward improved disease diagnosis and prognosis.
authors.affiliation: †CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.|§University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.affiliation: †CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
authors.forename: Bidou
authors.initials: B
authors.lastname: Wang
authors.affiliation: †CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.|‡Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444, China.|§University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Meng
authors.affiliation: †CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.affiliation: †CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.|§University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D035683:MicroRNAs; D004247:DNA