title: Angiotensin processing activities in the venom of Thalassophryne nattereri.
abstract: The venom of marine animals is a rich source of compounds with remarkable functional specificity and diversity. Thalassophryne nattereri is a small venomous fish inhabiting the northern and northeastern coast of Brazil, and represents a relatively frequent cause of injuries. Its venom causes severe inflammatory response followed frequently by the necrosis of the affected area. This venom presents characterized components such as proteases (Natterins 1-4) and a lectin (Nattectin) with complex effects on the human organism. A specific inhibitor of tissue kallikrein (TKI) reduces the nociception and the edema caused by the venom in mice. Our study sought to investigate the proteolytic activities against vasopeptides Angiotensin I, Angiotensin II, Angiotensin 1-9 and Bradykinin. The venom indicated angiotensin conversion against angiotensin I, as well as kininase against bradykinin. Captopril conducted the total inhibition of the converting activity, featuring the first report of ACE activity in fish venoms.
authors.affiliation: Instituto de Química e Biotecnologia- Universidade Federal de Alagoas, Maceió, Brazil.
authors.forename: Humberto de Araújo
authors.initials: Hde A
authors.lastname: Tenório
authors.affiliation: Instituto de Química e Biotecnologia- Universidade Federal de Alagoas, Maceió, Brazil.
authors.forename: Maria Elizabeth da Costa
authors.initials: ME
authors.lastname: Marques
authors.affiliation: Instituto de Química e Biotecnologia- Universidade Federal de Alagoas, Maceió, Brazil.
authors.forename: Sonia Salgueiro
authors.initials: SS
authors.lastname: Machado
authors.affiliation: Instituto de Química e Biotecnologia- Universidade Federal de Alagoas, Maceió, Brazil. Electronic address: hugobqi@yahoo.com.br.
authors.forename: Hugo Juarez Vieira
authors.initials: HJ
authors.lastname: Pereira
mesh_terms: D000803:Angiotensin I / Q000037:antagonists & inhibitors / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000809:Angiotensins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D024541:Batrachoidiformes; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000378:metabolism; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005397:Fish Venoms / Q000737:chemistry*; D005400:Fishes, Poisonous; D051379:Mice
chemical_list: D000809:Angiotensins; D005397:Fish Venoms; D000804:Angiotensin II; D000803:Angiotensin I; D001920:Bradykinin
keywords: Angiotensin converting enzyme; Bradykinin; Kininase; Thalassophryne nattereri