title: High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium.
abstract: In order to investigate the potential of short antimicrobial peptides (AMPs) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg/mL to 0.5 mg/mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy (CLSM). The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Ji-yin
authors.initials: JY
authors.lastname: Li
authors.affiliation: Department of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors.forename: Xue-jin
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Wang
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Ying
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.
authors.forename: Guo-wu
authors.initials: GW
authors.lastname: Ma
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015921:Dental Implants / Q000382:microbiology*; D003764:Dental Materials; D004305:Dose-Response Relationship, Drug; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000502:physiology*; D014025:Titanium; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D015921:Dental Implants; D003764:Dental Materials; C009495:titanium dioxide; D014025:Titanium