title: Recent advances in proteomic profiling of human blood: clinical scope.
abstract: A chromosome-centric approach in combination with targeted selected reaction monitoring-mass spectrometry analysis is one of the main approaches to study the human proteome. Measuring the size of the human plasma proteome includes both definition of all forms of proteins and quantitative measuring of the content of each protein form. The algorithm for measuring the proteome of canonical (master) proteins of chromosome 18 was created by combining a chromosome-centric approach and selected reaction monitoring-mass spectrometry. It can be scaled for all chromosomes to measure master proteins in the human blood plasma. Establishment of selected reaction monitoring-mass spectrometry diagnostic assays for quantitative measuring of the proteins associated with the development of diseases is a practical result.
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya Street 10, Moscow, 119121, Russian Federation.
authors.forename: Alexander
authors.initials: A
authors.lastname: Archakov
authors.forename: Andrey
authors.initials: A
authors.lastname: Lisitsa
authors.forename: Elena
authors.initials: E
authors.lastname: Ponomarenko
authors.forename: Victor
authors.initials: V
authors.lastname: Zgoda
mesh_terms: D002887:Chromosomes, Human, Pair 18; D020869:Gene Expression Profiling; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010949:Plasma / Q000737:chemistry*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: clinical application; human proteome; mass spectrometry; proteome depth; proteome size; proteome width; selected reaction monitoring; sensitivity; shotgun mass spectrometry