title: Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds.
abstract: Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10days in consistent dosages averaging 80.5±12.5(SD)ng/mL. Moreover, the BDNF released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages.
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Lynam
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Dena
authors.initials: D
authors.lastname: Shahriari
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Kayla J
authors.initials: KJ
authors.lastname: Wolf
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Angart
authors.affiliation: Center for Neural Repair, University of California, San Diego, CA 92093, United States.
authors.forename: Jacob
authors.initials: J
authors.lastname: Koffler
authors.affiliation: Center for Neural Repair, University of California, San Diego, CA 92093, United States.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Tuszynski
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Christina
authors.initials: C
authors.lastname: Chan
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Patrick
authors.initials: P
authors.lastname: Walton
authors.affiliation: Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D048091:Guided Tissue Regeneration; D051379:Mice; D041681:NIH 3T3 Cells; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D020813:Receptor, trkB / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020099:Coated Materials, Biocompatible; D012685:Sepharose; D020813:Receptor, trkB
keywords: Agarose; BDNF; Controlled release; Hydrogel; Nerve