title: On archaeal homologs of the human RNase P proteins Pop5 and Rpp30 in the hyperthermophilic archaeon Thermococcus kodakarensis.
abstract: The ribonuclease P (RNase P) proteins TkoPop5 and TkoRpp30, homologs of human Pop5 and Rpp30, respectively, in the hyperthermophilic archaeon Thermococcus kodakarensis were prepared and characterized with respect to pre-tRNA cleavage activity using the reconstitution system of the well-studied Pyrococcus horikoshii RNase P. The reconstituted particle containing TkoPop5 in place of the P. horikoshii counterpart PhoPop5 retained pre-tRNA cleavage activity comparable to that of the reconstituted P. horikoshii RNase P, while that containing TkoRpp30 instead of its corresponding protein PhoRpp30 had slightly lower activity than the P. horikoshii RNase P. Moreover, we determined crystal structures of TkoRpp30 alone and in complex with TkoPop5. Like their P. horikoshii counterparts, whose structures were solved previously, TkoRpp30 and TkoPop5 fold into TIM barrel and RRM-like fold, respectively. This finding demonstrates that RNase P proteins in T. kodakarensis and P. horikoshii are interchangeable and that their three-dimensional structures are highly conserved.
authors.affiliation: a Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Science , Kyushu University , Fukuoka , Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Suematsu
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Ueda
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kakuta
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D001324:Autoantigens / Q000737:chemistry* / Q000378:metabolism; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D043902:Pyrococcus horikoshii / Q000201:enzymology; D043262:Ribonuclease P / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D019712:Thermococcus / Q000201:enzymology*
chemical_list: D001324:Autoantigens; C104646:RPP30 protein, human; C000605944:Pop5 protein, human; D043262:Ribonuclease P
keywords: Thermococcus kodakarensis; crystal structure; hyperthermophilic archaea; ribonuclease P; tRNA precursor