title: New UHPLC-QqQ-MS/MS method for quantitative and qualitative determination of free phytoprostanes in foodstuffs of commercial olive and sunflower oils.
abstract: In this work, we propose a new quick and accurate analytical method by UHPLC-QqQ-MS/MS which is able to identify free phytoprostanes in olive and refined sunflower oils. The recovery provided high extraction efficiencies ranging from 102.90% to 140.64% using Strata-XAW cartridge. The intra-day and inter-day variations for all target compounds ranged from 2.24% to 13.64% and 0.01% to 13.69%, respectively, and the accuracies for these parameters varied from 80.33% to 119.64% and from 80.34% to 119.90%, respectively. Results obtained reflect that refined sunflower presented more series of phytoprostanes and a 20 and 8-fold higher quantity than two types of olive oil: Extra virgin olive oil and olive oil, (containing half virgin extra olive oil and half refined olive oil). The manufacture process could be the key for the different phytoprostane production since most of the plant oils are subjected to a refining treatment.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Jacinta
authors.initials: J
authors.lastname: Collado-González
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Medina
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University Montpellier I and II - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University Montpellier I and II - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Guy
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University Montpellier I and II - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: Department of Irrigation, CEBAS-CSIC, P.O. Box 164, Espinardo, E-30100 Murcia, Spain.
authors.forename: Arturo
authors.initials: A
authors.lastname: Torrecillas
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain. Electronic address: angelgil@cebas.csic.es.
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D057230:Limit of Detection; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis*; D000074242:Sunflower Oil; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005231:Fatty Acids, Unsaturated; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil
keywords: Mass spectrometry; Olive oil; Phytoprostanes; Sunflower oil; UHPLC