title: An aquaporin protein is associated with drought stress tolerance.
abstract: Water channel proteins known as aquaporins (AQPs) regulate the movement of water and other small molecules across plant vacuolar and plasma membranes; they are associated with plant tolerance of biotic and abiotic stresses. In this study, a PIP type AQPs gene, designated as GoPIP1, was cloned from Galega orientalis, a high value leguminous forage crop. The GoPIP1 gene consists of an 870 bp open reading frame encoding a protein of 289 amino acids, and belongs to the PIP1 subgroup of the PIP subfamily. The transcript level of GoPIP1 was higher in the root of G. orientalis than in the leaf and stem. The level of GoPIP1 transcript increased significantly when treated with 200 mM NaCl or 20% polyethylene glycol (PEG) 6000. Transient expression of GoPIP1 in onion epidermal cells revealed that the GoPIP1 protein was localized to the plasma membrane. Over-expression of GoPIP1 increased the rosette/root ratio and increased sensitivity to drought in transgenic Arabidopsis plants. However, GoPIP1 over-expression in Arabidopsis had no significant effect under saline condition. The present data provides a gene resource that contributes to furthering our understanding of water channel protein and their application in plant stress tolerance.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China; Institute of Grassland Research, Chinese Academy of Agricultural Sciences / SharaQin Key Wild Scientific Monitoring Station for Forage Resources of Ministry of Agriculture, 120 Wulanchabu East Street, Saihan District, Hohhot 010010, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College Animal Science & Technology, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ban
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wen
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg 190000, Russia.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Dzyubenko
authors.affiliation: N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg 190000, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chapurin
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Gao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China. Electronic address: wangxuemin@caas.cn.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000064:Acclimatization / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003681:Dehydration; D055864:Droughts; D029881:Galega / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D054712:Salinity; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological
chemical_list: D020346:Aquaporins; D010940:Plant Proteins; C110065:major intrinsic protein, plant
keywords: Abiotic stress; Aquaporin; Galega orientalis L.; GoPIP1; Transgenic Arabidopsis