title: Which is the best route of administration for cell therapy in experimental model of small-for size syndrome in rats?
abstract: OBJECTIVE
  To evaluate which is the best route of administration for cell therapy in experimental rat model of small-for size syndrome.
  
  
  METHODS
  A total of 40 rats underwent partial hepatectomy (70%) that induces the small-for-size syndrome and were divided into four groups of route administration: intravenous, intraperitoneal, enteral and tracheal. The small-for-size syndrome model was designed with extended partial hepatectomy (70%). The animals were divided into four groups of routes administration: intravenous (n=10) - intravenously through the dorsal vein of the penis; intraperitoneal (n=10) - intraperitoneally in the abdominal cavity; enteral (n=10) - oroenteral with the placement of a number 4 urethral probe and maintained at third duodenal portion; tracheal (n=10) - after tracheal intubation. We track the animals and monitor them for 21 days; during this follow-up we evaluated the result of cell therapy application tracking animals using ultrasound, radiography and PET-scan. Statistical analysis was performed using GraphPad Prism Software(r). Differences were considered significant with the p<0.05. Data are presented as the median and variation for continuous variables. Comparisons between groups were made using analysis of the imaging test by the researchers.
  
  
  RESULTS
  All four groups underwent partial hepatectomy of 70% liver tissue targeting the same weight of resected liver. Initially the PET-scan tests showed similarity in administered cells by different routes. However, in few days the route of intravenous administration showed to be the most appropriated to lead cells to the liver followed by enteral. The tracheal and peritoneal routes were not as much successful for this goal.
  
  
  CONCLUSIONS
  The intravenous route is the best one to cell therapy in experimental rat model of small-for size-syndrome.
authors.affiliation: Medical Investigation Laboratory, Department of Gastroenterology, School of Medicine, University of Sao Paulo, Brazil.
authors.forename: Lucas Souto
authors.initials: LS
authors.lastname: Nacif
authors.affiliation: Laboratory of Biochemistry and Biophysics, Biochemistry in Biotechnology Center, Butantan Institute, Sao Paulo, SP, Brazil.
authors.forename: Amanda Olivotti
authors.initials: AO
authors.lastname: Ferreira
authors.affiliation: Laboratory of Biochemistry and Biophysics, Biochemistry in Biotechnology Center, Butantan Institute, Sao Paulo, SP, Brazil.
authors.forename: Durvanei Augusto
authors.initials: DA
authors.lastname: Maria
authors.affiliation: LIM 37, Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Márcia Saldanha
authors.initials: MS
authors.lastname: Kubrusly
authors.affiliation: LIM 37, Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Nilza
authors.initials: N
authors.lastname: Molan
authors.affiliation: LIM 37, Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Eleazar
authors.initials: E
authors.lastname: Chaib
authors.affiliation: LIM 37, Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Luiz Carneiro
authors.initials: LC
authors.lastname: D'Albuquerque
authors.affiliation: LIM 37, Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Wellington
authors.initials: W
authors.lastname: Andraus
mesh_terms: D000818:Animals; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D004195:Disease Models, Animal; D004333:Drug Administration Routes; D006498:Hepatectomy; D008099:Liver / Q000737:chemistry; D008107:Liver Diseases / Q000628:therapy*; D008115:Liver Regeneration / Q000502:physiology*; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D009929:Organ Size; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D033581:Stem Cell Transplantation / Q000379:methods*; D013577:Syndrome; D013997:Time Factors