title: Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation.
abstract: A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6 wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea.
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Busan, Republic of Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Kim
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Ham
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea.
authors.forename: Sang In
authors.initials: SI
authors.lastname: Park
authors.affiliation: Department of Molecular Physiology, College of Pharmacy, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Department of Life Systems, Sookmyung Women's University, Yongsan-gu Seoul, Republic of Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Microbiology, College of Natural Science, Pusan National University, Busan, Republic of Korea.
authors.forename: Jihwan
authors.initials: J
authors.lastname: Hwang
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea.
authors.forename: Moon-Seo
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Seung-Schik
authors.initials: SS
authors.lastname: Yoo
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea.
authors.forename: Lee-So
authors.initials: LS
authors.lastname: Maeng
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Busan, Republic of Korea. Electronic address: wchang1975@pusan.ac.kr.
authors.forename: Woochul
authors.initials: W
authors.lastname: Chang
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea. Electronic address: nm@catholic.ac.kr.
authors.forename: Yong-An
authors.initials: YA
authors.lastname: Chung
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000000981:diagnostic imaging*; D014464:Ultrasonic Therapy / Q000379:methods*; D000069453:Ultrasonic Waves; D014463:Ultrasonography
keywords: Bone regeneration; Calvarial bone fracture; Focused low-intensity pulsed ultrasound; Osteocyte proliferation