title: Porphyromonas gingivalis B cell Antigen Epitope Vaccine, pIRES-ragB'-mGITRL, Promoted RagB-Specific Antibody Production and Tfh Cells Expansion.
abstract: The outer membrane protein RagB is one of the major virulence factors of Porphyromonas gingivalis (P. gingivalis). To prevent periodontitis and associated systemic diseases induced by P. gingivalis, we built B cell antigen epitope vaccine characterized by pIRES-ragB'-mGITRL to induce a protective immune responses. The B cell antigen epitope and scrambled peptide of ragB were predicted, cloned into pIRES and constructed pIRES-ragB', pIRES-scrambled epitopes and pIRES-ragB'-mGITRL. pIRES-ragB'-mGITRL was transfected into COS-7 cells. Subsequently, the 6-week-old female BALB/c mice were challenged by P. gingivalis following three time immunization by pIRES, pIRES-ragB', pIRES-scrambled epitopes and pIRES-ragB'-mGITRL. The levels of RagB-specific antibody in the serum and Tfh cells in the spleen were measured by ELISA and flow cytometry, respectively. And higher levels of RagB-specific IgG were produced in the immunized mice with pIRES-ragB'-mGITRL. Additionally, the number of Tfh cells was also expanded and lesions were diminished in pIRES-ragB'-mGITRL mice comparing with control groups. Our results clearly demonstrated that P. gingivalis B cell antigen epitope vaccine, pIRES-ragB'-mGITRL, could induce protective immune responses. Furthermore, our data also indicated that pIRES-ragB'-mGITRL was a potential therapeutic vaccine against P. gingivalis.
authors.affiliation: Department of Stomatology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Stomatology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.
authors.forename: D
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Stomatology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.
authors.forename: P
authors.initials: P
authors.lastname: She
authors.affiliation: Department of Immunology & Laboratory Immunology, Jiangsu University, Zhenjiang, China.
authors.forename: P
authors.initials: P
authors.lastname: Ni
authors.affiliation: Department of Immunology & Laboratory Immunology, Jiangsu University, Zhenjiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Immunology & Laboratory Immunology, Jiangsu University, Zhenjiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Immunology & Laboratory Immunology, Jiangsu University, Zhenjiang, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Su
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D016866:Bacteroidaceae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D019556:COS Cells; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008807:Mice, Inbred BALB C; D010518:Periodontitis / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D007074:Immunoglobulin G; C484346:RagB protein, bacteria; D011947:Receptors, Antigen, B-Cell; C478966:Tnfsf18 protein, mouse; D048069:Tumor Necrosis Factors; D019444:Vaccines, DNA