title: Surveillance of artemisinin resistance in Plasmodium falciparum in India using the kelch13 molecular marker.
abstract: Malaria treatment in Southeast Asia is threatened with the emergence of artemisinin-resistant Plasmodium falciparum. Genome association studies have strongly linked a locus on P. falciparum chromosome 13 to artemisinin resistance, and recently, mutations in the kelch13 propeller region (Pfk-13) were strongly linked to resistance. To date, this information has not been shown in Indian samples. Pfk-13 mutations were assessed in samples from efficacy studies of artemisinin combination treatments in India. Samples were PCR amplified and sequenced from codon 427 to 727. Out of 384 samples, nonsynonymous mutations in the propeller region were found in four patients from the northeastern states, but their presence did not correlate with ACT treatment failures. This is the first report of Pfk-13 point mutations from India. Further phenotyping and genotyping studies are required to assess the status of artemisinin resistance in this region.
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Neelima
authors.initials: N
authors.lastname: Mishra
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Prajapati
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Kaitholia
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Ram Suresh
authors.initials: RS
authors.lastname: Bharti
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Bina
authors.initials: B
authors.lastname: Srivastava
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Sobhan
authors.initials: S
authors.lastname: Phookan
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Anupkumar R
authors.initials: AR
authors.lastname: Anvikar
authors.affiliation: National Institute of Malaria Research, New Delhi, India.
authors.forename: Vas
authors.initials: V
authors.lastname: Dev
authors.affiliation: National Vector Borne Disease Control Programme, Delhi, India.
authors.forename: Gagan Singh
authors.initials: GS
authors.lastname: Sonal
authors.affiliation: National Vector Borne Disease Control Programme, Delhi, India.
authors.forename: Akshay Chandra
authors.initials: AC
authors.lastname: Dhariwal
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.affiliation: National Institute of Malaria Research, New Delhi, India neenavalecha@gmail.com.
authors.forename: Neena
authors.initials: N
authors.lastname: Valecha
mesh_terms: D037621:Artemisinins / Q000494:pharmacology*; D004094:Dihydropteroate Synthase / Q000235:genetics / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics*; D007194:India; D008969:Molecular Sequence Data; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D037621:Artemisinins; D015800:Protozoan Proteins; C031327:artemisinin; D004094:Dihydropteroate Synthase