title: Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions.
abstract: Reprogramming somatic cells into a pluripotent state involves the overexpression of transcription factors leading to a series of changes that end in the formation of induced pluripotent stem cells (iPSCs). These iPSCs have a wide range of potential uses from drug testing and in vitro disease modelling to personalized cell therapies for patients. While viral methods for reprogramming factor delivery have been traditionally preferred due to their high efficiency, it is now possible to generate iPSCs using nonviral methods at similar efficiencies. We developed a robust reprogramming strategy that combines episomal plasmids and the use of commercially available animal free reagents that can be easily adapted for the GMP manufacture of clinical grade cells.
authors.affiliation: UCL Cancer Institute, University College London, London, WC1E 6BT, UK.
authors.forename: Sara
authors.initials: S
authors.lastname: Caxaria
authors.affiliation: UCL Cancer Institute, University College London, London, WC1E 6BT, UK.
authors.forename: Susanne
authors.initials: S
authors.lastname: Arthold
authors.affiliation: UCL Cancer Institute, University College London, London, WC1E 6BT, UK.
authors.forename: Amit C
authors.initials: AC
authors.lastname: Nathwani
authors.affiliation: Centre for Paediatrics, Barts and The London Medical School, Blizard Institute, Queen Mary University of London, London, E1 2AT, UK. p.goh@qmul.ac.uk.
authors.forename: Pollyanna Agnes
authors.initials: PA
authors.lastname: Goh
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D065150:Cellular Reprogramming; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D022861:Hermanski-Pudlak Syndrome / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D012692:Serial Passage; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019076:Transgenes
chemical_list: D000577:Amides; D000954:Antigens, Surface; D036341:Intercellular Signaling Peptides and Proteins; D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D011725:Pyridines; C519803:TRA-1-81 antigen, human; C108830:Y 27632; D000469:Alkaline Phosphatase
keywords: Episomal plasmids; Induced pluripotent stem cells; Reprogramming