title: Fluorescence axial nanotomography with plasmonics.
abstract: We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.
authors.affiliation: Department of Physics, King's College London, Strand, London WC2R 2LS, UK. david.r.richards@kcl.ac.uk.
authors.forename: Nicholas I
authors.initials: NI
authors.lastname: Cade
authors.forename: Gilbert O
authors.initials: GO
authors.lastname: Fruhwirth
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Krasavin
authors.forename: Tony
authors.initials: T
authors.lastname: Ng
authors.forename: David
authors.initials: D
authors.lastname: Richards
mesh_terms: D045744:Cell Line, Tumor; D004705:Endocytosis; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000502:physiology
chemical_list: D019718:Receptors, CXCR4; D049452:Green Fluorescent Proteins; D006046:Gold