title: [Methods of phenotypic determination of New Delhi metallo-beta-lactamase (NDM-1) in Klebsiella penumoniae isolated in Slovakia].
abstract: OBJECTIVE
  Reported is the first isolation and phenotypic determination of Klebsiella pneumoniae producing New Delhi metallo-beta-lactamase (NDM-1) isolated from patients in the Slovak Republic.
  
  
  METHODS
  Between 27 October 2012 and 22 January 2013, twenty-five isolates of Klebsiella pneumoniae collected from 5 patients were identified with MIC of meropenem ≥ 32 mg/L and MIC of ertapenem ≥ 4 mg/L in screening tests. Next, all isolates were assessed with the modified Hodge test, combined disk test with EDTA, double disk synergy test with EDTA and MBL E-test. To confirm production of MBL in isolated strains of Klebsiella pneumoniae, all strains were sent to the National Reference Center for Antibiotic Resistance in Bratislava.
  
  
  RESULTS
  All strains were positive in all phenotypic tests. In the first carbapenem-resistant isolate, NDM-1 production was confirmed by PCR amplification, sequencing and comparison with the GenBank.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first case of isolation of NDM-1 from Klebsiella pneumoniae in the Slovak Republic. As of 31 January 2014, with well-established and strict epidemiological and preventive measures, there was no further spread or another outbreak of NDM-1 producing Enterobacteriaceae in Louis Pasteur University Hospital in Košice.
authors.affiliation: Department of medical microbiology and clinical microbiology, University hospital of P. J. Šafařik, Košice, Slovak Republic, e-mail: eva.kendrovska@gmail.com.
authors.forename: Eva
authors.initials: E
authors.lastname: Schréterová
authors.forename: Viktória
authors.initials: V
authors.lastname: Takáčová
authors.forename: Milan
authors.initials: M
authors.lastname: Nikš
authors.forename: Lýdia
authors.initials: L
authors.lastname: Pastvová
authors.forename: Ladislav
authors.initials: L
authors.lastname: Tomčo
authors.forename: Leonard
authors.initials: L
authors.lastname: Siegfried