title: [Change of expression of integrins on multipotent mesenchymal stromal cells isolated from human adipose tissue during long cultivation].
abstract: An expression ofintegrins on multipotent mesenchymal stromal cells (MMSCs), isolated from human subcutaneous adipose tissue during long cultivation was studied. Results of the comparative analysis of MMSCs on the 2nd and 17th passages of cultivation revealed considerable distinctions in expression of α1, α4 and α6 integrins on these cells. Strong decrease for 87.2 and 11.2%, quantities of the cells which have been positively stained by AT against CD49a (α1 integrin) and CD49d (α4 integrin), respectively was observed. The share of the cells which have been positively stained by AT against CD49f (α6 integrin) and CD49b (α2 integrin) on the contrary was increased during long cultivation by 9.9 and 2.3%, respectively. The high expression of β1 integrin (98%) on MMSCs, wasn't changed significantly in the course of long cultivation. As a result of induction of MMSCs to a differentiation in the osteogenic direction it was revealed that cells on the 17th passage of cultivation concede by efficiency of formation of cells of bone tissue and extracellular matrix in vitro to cells which induced on the 2nd passage. Thus, long cultivation of MMSCs which is required for building of cellular population after isolation, influences on a cytoskeleton and adhesive abilities of cells that it is necessary to consider when using them for cellular technologies, including for modeling of this or that tissue in three-dimensional scaffolds.
authors.forename: I P
authors.initials: IP
authors.lastname: Savchenkova
authors.forename: E A
authors.initials: EA
authors.lastname: Savchenkova
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D039423:Integrin alpha1 / Q000235:genetics* / Q000378:metabolism; D039441:Integrin alpha4 / Q000235:genetics* / Q000378:metabolism; D039503:Integrin alpha6 / Q000235:genetics* / Q000378:metabolism; D019012:Integrin beta1 / Q000235:genetics* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D061251:Primary Cell Culture; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D039423:Integrin alpha1; D039503:Integrin alpha6; D019012:Integrin beta1; D039441:Integrin alpha4