title: Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes.
abstract: Parameters such as total number of coelomocytes, riboflavin content in coelomocytes, expression of genes implied in metal homeostasis, and detoxification mechanisms can be used as biomarkers to assess the impact of metals on annelids. Defense biomarkers (detoxification gene expressions and coelomocyte parameters) were investigated in the ecotoxicologically important species Eisenia andrei following in vivo exposure to 5 different metals (zinc, copper, nickel, lead, and cadmium) at known concentrations. Coelomocyte numbers and riboflavin content were not affected by metallic exposure, but metal-specific gene expression variations were evidenced.
authors.affiliation: Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Homa
authors.affiliation: Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland.|Environmental Axis, Lille 1 University, Villeneuve d'Ascq, France.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rorat
authors.affiliation: Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kruk
authors.affiliation: Environmental Axis, Lille 1 University, Villeneuve d'Ascq, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Cocquerelle
authors.affiliation: Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Plytycz
authors.affiliation: Environmental Axis, Lille 1 University, Villeneuve d'Ascq, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Vandenbulcke
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects*; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism / Q000633:toxicity*; D009835:Oligochaeta / Q000145:classification / Q000187:drug effects*; D010802:Phylogeny; D012256:Riboflavin / Q000032:analysis; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D019216:Metals, Heavy; D012989:Soil Pollutants; D002104:Cadmium; D008668:Metallothionein; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase; D012256:Riboflavin
keywords: Coelomocytes; Eisenia andrei; Heavy metals; metallothionein (mt); phytochelatin synthase (pcs)