title: Effects of anesthetic agents on in vivo axonal HCN current in normal mice.
abstract: OBJECTIVE
  The objective was to study the in vivo effects of anesthetic agents on peripheral nerve excitability.
  
  
  METHODS
  Normal male mice were anesthetized by either isoflurane inhalation or a combination of medetomidine, midazolam, and butorphanol intraperitoneal injection ("triple agents"). Immediately after induction, the tail sensory nerve action potential was recorded and its excitability was monitored.
  
  
  RESULTS
  Under both anesthetic protocols, there was an interval excitability change by long hyperpolarizing currents. There was greater threshold reduction approximately 30min post induction, in comparison to immediately post induction. Other excitability parameters were stable over time. Modeling suggested interval suppression of internodal H conductance or leak current.
  
  
  CONCLUSIONS
  Anesthetic agents affected responses to long hyperpolarizing currents.
  
  
  CONCLUSIONS
  Axonal excitability during intraoperative monitoring may be affected by anesthetic agents. Interpretation of interval excitability changes under anesthesia requires caution, especially with long hyperpolarizing currents.
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Osaki
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan. Electronic address: hnodera@tokushima-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nodera
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Chimeglkham
authors.initials: C
authors.lastname: Banzrai
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Endo
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takayasu
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Mori
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Shimatani
authors.affiliation: Department of Neurology, Tokushima University, Tokushima, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kaji
mesh_terms: D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology*; D000777:Anesthetics / Q000493:pharmacokinetics*; D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D000777:Anesthetics
keywords: Anesthetics; Axonal excitability; HCN channel