title: Comparison analysis in synchronous and metachronous metastatic colorectal cancer based on microarray expression profile.
abstract: OBJECTIVE
  Colorectal cancer (CRC) is one of the most common malignancies, and liver metastasis is one of the major causes of death of CRC. This study aimed to compare the genetic difference between metachronous lesions (MC) and synchronous lesions (SC) and explore the molecular pathology of CRC metastasis.
  
  
  METHODS
  Microarray expression profile data (GSE10961) including 8 MC and 10 SC was downloaded from Gene Expression Omnibus. The differentially expressed genes (DEGs) between the two groups were identified based on T test. Furthermore, GO enrichment analysis was performed for the down-regulated DEGs using DAVID. Finally, Classify validation of known CRC genes based on previous studies between MC and SC samples was conducted.
  
  
  RESULTS
  Total of 36 DEGs including 35 down-regulated DEGs and 1 up-regulated DEGs were identified. The expressional differences of the 5 informative oncogenes: EGFr, PIK3R1, PTGS2 (COX-2), PTGS1 (COX1), and ALOX5AP between SC and MC were really tiny.
  
  
  CONCLUSIONS
  Some DEGs, such as NFAT5, OLR1, ERAP2, HOXC6 and TWIST1 might play crucial roles in the regulation of CRC metastasis (both SC and MC) and by disrupting some pathways. However, our results indeed demand further research and experiment.
authors.forename: Da-dong
authors.initials: DD
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Yu-liang
authors.initials: YL
authors.lastname: Tu
authors.forename: Xiang-long
authors.initials: XL
authors.lastname: Tan
authors.forename: Zi-man
authors.initials: ZM
authors.lastname: Zhu
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Han
authors.forename: Chun-qing
authors.initials: CQ
authors.lastname: Dou
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Zeng
authors.forename: Jing-wang
authors.initials: JW
authors.lastname: Tan
authors.forename: Jun-dong
authors.initials: JD
authors.lastname: Du
authors.forename: Hua-bou
authors.initials: HB
authors.lastname: Jiao
authors.forename: Shou-wang
authors.initials: SW
authors.lastname: Cai
mesh_terms: D015179:Colorectal Neoplasms / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D009378:Neoplasms, Multiple Primary / Q000473:pathology*; D016609:Neoplasms, Second Primary / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome