title: Polydiacetylene nanovesicles as carriers of natural phenylpropanoids for creating antimicrobial food-contact surfaces.
abstract: The ultimate goal of this study was developing antimicrobial food-contact materials based on natural phenolic compounds using nanotechnological approaches. Among the methyl-β-cyclodextrin-encapsulated phenolics tested, curcumin showed by far the highest activity toward Escherichia coli with a minimum inhibitory concentration of 0.4 mM. Curcumin was enclosed in liposome-type polydiacetylene/phosholipid nanovesicles supplemented with N-hydroxysuccinimide and glucose. The fluorescence spectrum of the nanovesicles suggested that curcumin was located in their bilayer region. Free-suspended nanovesicles tended to bind to the bacterial surface and demonstrated bactericidal activity toward Gram-negative (E. coli) and vegetative cells of Gram-positive (Bacillus cereus) bacteria reducing their counts from 5 log CFU mL(-1) to an undetectable level within 8 h. The nanovesicles were covalently bound to silanized glass. Incubation of E. coli and B. cereus with nanovesicle-coated glass resulted in a 2.5 log reduction in their counts. After optimization this approach can be used for controlling microbial growth, cross-contamination, and biofilm formation on food-contacting surfaces.
authors.affiliation: College of Science, 1245 Lincoln Drive, Neckers 157A, Southern Illinois University , Carbondale Illinois 62901-4403, United States.
authors.forename: Navneet
authors.initials: N
authors.lastname: Dogra
authors.forename: Ruplal
authors.initials: R
authors.lastname: Choudhary
authors.forename: Punit
authors.initials: P
authors.lastname: Kohli
authors.forename: John D
authors.initials: JD
authors.lastname: Haddock
authors.forename: Sanjaysinh
authors.initials: S
authors.lastname: Makwana
authors.forename: Batia
authors.initials: B
authors.lastname: Horev
authors.forename: Yakov
authors.initials: Y
authors.lastname: Vinokur
authors.forename: Samir
authors.initials: S
authors.lastname: Droby
authors.forename: Victor
authors.initials: V
authors.lastname: Rodov
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects / Q000254:growth & development; D003474:Curcumin / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D008826:Microbial Sensitivity Tests; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D003474:Curcumin
keywords: Bacillus cereus; Escherichia coli; antimicrobial surfaces; bactericidal; curcumin; glass; liposomes; methyl-β-cyclodextrin; nanoparticles; phenylpropanoids