title: Myoblast differentiation of human mesenchymal stem cells on graphene oxide and electrospun graphene oxide-polymer composite fibrous meshes: importance of graphene oxide conductivity and dielectric constant on their biocompatibility.
abstract: Recently graphene and graphene based composites are emerging as better materials to fabricate scaffolds. Addition of graphene oxide (GO) nanoplatelets (GOnPs) in bioactive polymers was found to enhance its conductivity (σ) and, dielectric permittivity (ϵ) along with biocompatibility. In this paper, human cord blood derived mesenchymal stem cells (CB-hMSCs) were differentiated to skeletal muscle cells (hSkMCs) on spin coated thin GO sheets composed of GOnPs and on electrospun fibrous meshes of GO-PCL (poly-caprolactone) composite. Both substrates exhibited excellent myoblast differentiations and promoted self-alignedmyotubesformation similar to natural orientation. σ, ϵ, microstructural and vibration spectroscopic studies were carried out for the characterizations of GO sheet and the composite scaffolds. Significantly enhanced values of both σ and ϵ of the GO-PCL composite were considered to provide favourable cues for the formation of superior multinucleated myotubes on the electrospun meshes compared to those on thin GO sheets. The present results demonstrated that both substrates might be used as potential candidates for CB-hMSCs differentiation and proliferation for human skeletal muscle tissue regeneration.
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha-769008, India.
authors.forename: Biswadeep
authors.initials: B
authors.lastname: Chaudhuri
authors.affiliation: Department of Solid State Physics, Indian Association for the Cultivation of Science, Kolkata-700032, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Bhadra
authors.affiliation: University of Twente, Tissue Regeneration Department, Drienerlolaan 5, 7522NB Enschede, The Netherlands.|Maastricht University, MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moroni
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha-769008, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Pramanik
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004126:Dimethylformamide / Q000494:pharmacology; D004553:Electric Conductivity; D005434:Flow Cytometry; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D032446:Myoblasts / Q000166:cytology*; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D010087:Oxides; D011108:Polymers; D006108:Graphite; D004126:Dimethylformamide