title: A wheat lipid transfer protein (TdLTP4) promotes tolerance to abiotic and biotic stress in Arabidopsis thaliana.
abstract: Lipid transfer proteins (LTPs) are members of the family of pathogenesis-related proteins (PR-14) that are believed to be involved in plant defense responses. In this study, we report the isolation and characterization of a novel gene TdLTP4 encoding an LTP protein from durum wheat [Triticum turgidum L. subsp. Durum Desf.]. Molecular Phylogeny analyses of wheat TdLTP4 gene showed a high identity to other plant LTPs. Predicted three-dimensional structural model revealed the presence of six helices and nine loop turns. Expression analysis in two local durum wheat varieties with marked differences in salt and drought tolerance, revealed a higher transcript accumulation of TdLTP4 under different stress conditions in the tolerant variety, compared to the sensitive one. The overexpression of TdLTP4 in Arabidopsis resulted in a promoted plant growth under various stress conditions including NaCl, ABA, JA and H2O2 treatments. Moreover, the LTP-overexpressing lines exhibit less sensitivity to jasmonate than wild-type plants. Furthermore, detached leaves from transgenic Arabidopsis expressing TdLTP4 gene showed enhanced fungal resistance against Alternaria solani and Botrytis cinerea. Together, these data provide the evidence for the involvement of TdLTP4 gene in the tolerance to both abiotic and biotic stresses in crop plants.
authors.affiliation: Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax/ University of Sfax, BP "1177", 3018 Sfax, Tunisia.
authors.forename: Hela
authors.initials: H
authors.lastname: Safi
authors.affiliation: Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax/ University of Sfax, BP "1177", 3018 Sfax, Tunisia.
authors.forename: Walid
authors.initials: W
authors.lastname: Saibi
authors.affiliation: Laboratoire de biochimie, environnement et agroalimentaire, Université Hassan II-Mohammedia, Faculté des Sciences et techniques, BP 146, Mohammedia 20650, Maroc.
authors.forename: Meryem Mrani
authors.initials: MM
authors.lastname: Alaoui
authors.affiliation: Laboratoire de biochimie, environnement et agroalimentaire, Université Hassan II-Mohammedia, Faculté des Sciences et techniques, BP 146, Mohammedia 20650, Maroc.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Hmyene
authors.affiliation: Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax/ University of Sfax, BP "1177", 3018 Sfax, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Masmoudi
authors.affiliation: Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax/ University of Sfax, BP "1177", 3018 Sfax, Tunisia.
authors.forename: Moez
authors.initials: M
authors.lastname: Hanin
authors.affiliation: Plant Protection and Improvement Laboratory, Centre of Biotechnology of Sfax/ University of Sfax, BP "1177", 3018 Sfax, Tunisia. Electronic address: faical.brini@cbs.rnrt.tn.
authors.forename: Faïçal
authors.initials: F
authors.lastname: Brini
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological / Q000235:genetics*; D052179:Antigens, Plant / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D055864:Droughts; D005658:Fungi; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D054883:Oxylipins / Q000378:metabolism; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C030363:lipid transfer protein; C511920:lipid transfer proteins, plant; D012965:Sodium Chloride; C011006:jasmonic acid; D000040:Abscisic Acid; D006861:Hydrogen Peroxide
keywords: Abiotic and biotic stresses; Gene expression; Jasmonic acid; Lipid transfer protein; Transgenic Arabidopsis plants; Wheat