title: Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity.
abstract: Circular RNAs (circRNAs) have re-emerged as an interesting RNA species. Using deep RNA profiling in different mouse tissues, we observed that circRNAs were substantially enriched in brain and a disproportionate fraction of them were derived from host genes that encode synaptic proteins. Moreover, on the basis of separate profiling of the RNAs localized in neuronal cell bodies and neuropil, circRNAs were, on average, more enriched in the neuropil than their host gene mRNA isoforms. Using high-resolution in situ hybridization, we visualized circRNA punctae in the dendrites of neurons. Consistent with the idea that circRNAs might regulate synaptic function during development, many circRNAs changed their abundance abruptly at a time corresponding to synaptogenesis. In addition, following a homeostatic downscaling of neuronal activity many circRNAs exhibited substantial up- or downregulation. Together, our data indicate that brain circRNAs are positioned to respond to and regulate synaptic function.
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Xintian
authors.initials: X
authors.lastname: You
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Irena
authors.initials: I
authors.lastname: Vlatkovic
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Babic
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Tristan
authors.initials: T
authors.lastname: Will
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Epstein
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Georgi
authors.initials: G
authors.lastname: Tushev
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Güney
authors.initials: G
authors.lastname: Akbalik
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Mantian
authors.initials: M
authors.lastname: Wang
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Caspar
authors.initials: C
authors.lastname: Glock
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Quedenau
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Hou
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Sambandan
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Max Planck Institute for Brain Research, Department of Synaptic Plasticity, Max-von-Laue Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Schuman
authors.affiliation: Berlin Institute for Medical Systems Biology, Max-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity / Q000502:physiology*; D019581:Neuropil / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012313:RNA / Q000378:metabolism*; D000079962:RNA, Circular; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013569:Synapses / Q000235:genetics*
chemical_list: D000079962:RNA, Circular; D012313:RNA