title: [Modulation of GABA- and kainate-activated currents by metabotropic receptors in isolated rat cortical neurons].
abstract: Whole-cell patch-clamp recordings from isolated neurons from rat prefrontal cortex have been made to study GABAb and mGluR receptor modulation of currents induced by applications of GABA and kainate. The GABAb-receptor antagonist CGP-55845 (5 microM) enhanced the peak by 26 +/- 13% (n = 6) but had no effect on the steady-state of GABA-activated current. Bath application of GABAb-receptor agonist baclofen (50 microM) enhanced the GABAa currents by 9 +/- 2% (n = 8). Kainate-activated currents were not affected by baclofen. Both GABA-activated currents and kainate-activated currents were not affected by trans-ACPD (MGluR agonist). These results suggest that in cortex postsynaptic response of GABAa-receptors can be modulated by GABAb-receptors.
authors.forename: D V
authors.initials: DV
authors.lastname: Amakhin
authors.forename: V A
authors.initials: VA
authors.lastname: Popov
authors.forename: N P
authors.initials: NP
authors.lastname: Veselkin
mesh_terms: D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D005260:Female; D018756:GABA Antagonists / Q000494:pharmacology; D058786:GABA-B Receptor Agonists / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D010721:Phosphinic Acids / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018080:Receptors, GABA-B / Q000276:immunology*; D018094:Receptors, Metabotropic Glutamate; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D018690:Excitatory Amino Acid Agonists; D018756:GABA Antagonists; D058786:GABA-B Receptor Agonists; D018696:Neuroprotective Agents; D010721:Phosphinic Acids; D011412:Propanolamines; D018080:Receptors, GABA-B; D018094:Receptors, Metabotropic Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; C085075:CGP 55845A; D005680:gamma-Aminobutyric Acid; D001418:Baclofen; D007608:Kainic Acid