title: The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway.
abstract: Golgins are coiled-coil proteins that participate in membrane-tethering events at the Golgi complex. Golgin-mediated tethering is thought to be important for vesicular trafficking and Golgi organization. However, the degree to which individual golgins contribute to these processes is poorly defined, and it has been proposed that golgins act in a largely redundant manner. Previous studies on the golgin GMAP-210 (also known as TRIP11), which is mutated in the rare skeletal disorder achondrogenesis type 1A, have yielded conflicting results regarding its involvement in trafficking. Here, we re-investigated the trafficking role of GMAP-210, and found that it is indeed required for efficient trafficking in the secretory pathway. GMAP-210 acts at both the endoplasmic reticulum (ER)-to-Golgi intermediate compartment (ERGIC) and Golgi complex during anterograde trafficking, and is also required for retrograde trafficking to the ER. Using co-depletion experiments, we also found that GMAP-210 acts in a partially redundant manner with the golgin GM130 to ensure efficient anterograde cargo delivery to the cis-Golgi. In summary, our results indicate a role for GMAP-210 in several trafficking steps at the ER-Golgi interface, some of which are partially redundant with another golgin, namely GM130 (also known as GOLGA2).
authors.affiliation: Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Peristera
authors.initials: P
authors.lastname: Roboti
authors.affiliation: Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sato
authors.affiliation: Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK martin.lowe@manchester.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Lowe
mesh_terms: D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D003598:Cytoskeletal Proteins; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D021381:Protein Transport; D055571:Secretory Pathway
chemical_list: D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C109331:TRIP11 protein, human
keywords: ERGIC; Golgi; Golgin; Membrane traffic; Secretion