title: Reactive oxygen species involved in CT26 immunogenic cell death induced by Clostridium difficile toxin B.
abstract: Immunogenic cell death (ICD) is a new concept appeared in recent years. Despite growing interests of research on ICD, the circumstances that trigger immune responses against dying tumor cells remain largely unknown. It was demonstrated that recombinant Clostridium difficile toxin B (rTcdB) can induce ICD in intoxicated cells, but its mechanism remains unclear. This work aims at exploring whether reactive oxygen species (ROS) involved in rTcdB induced ICD using the chemical agent N-acetyl cysteine (NAC), diphenylene iodonium (DPI) and Antimycin A (Anti.A). The results suggested that ROS involved in rTcdB induced apoptosis and autophagy. DPI and Anti.A successfully inhibited the antitumor immune effect induced by rTcdB. As ICD is determined by a variety of factors, rTcdB is a potential tool for further exploring the circumstances that trigger ICD, which may offer us a good choice for designing the new chemotherapeutic drugs with immunogenic properties.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Sun
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Mao
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou, PR China. Electronic address: jufwang@scut.edu.cn.
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D001426:Bacterial Proteins / Q000378:metabolism* / Q000494:pharmacology; D001427:Bacterial Toxins / Q000378:metabolism* / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; C031815:toxB protein, Clostridium difficile
keywords: Apoptosis; Clostridium difficile toxin B; Immunogenic cell death; Reactive oxygen species