title: Evaluating the impact of sprouting conditions on the glucosinolate content of Brassica oleracea sprouts.
abstract: The glucosinolates content of brassica plants is a distinctive characteristic, representing a healthy advantage as many of these compounds are associated to antioxidant and anti-carcinogenic properties. Brassica sprouts are still an underutilized source of these bioactive compounds. In this work, four varieties of brassica sprouts (red cabbage, broccoli, Galega kale and Penca cabbage), including two local varieties from the North of Portugal, were grown to evaluate the glucosinolate profile and myrosinase activity during the sprouting. Also the influence of light/darkness exposure during sprouting on the glucosinolate content was assessed. Glucosinolate content and myrosinase activity of the sprouts was evaluated by HPLC methods. All sprouts revealed a higher content of aliphatic glucosinolates than of indole glucosinolates, contrary to the profile described for most of brassica mature plants. Galega kale sprouts had the highest glucosinolate content, mainly sinigrin and glucoiberin, which are recognized for their beneficial health effects. Penca cabbage sprouts were particularly richer in glucoraphanin, who was also one of the major compounds in broccoli sprouts. Red cabbage showed a higher content of progoitrin. Regarding myrosinase activity, Galega kale sprouts showed the highest values, revealing that the use of light/dark cycles and a sprouting phase of 7-9 days could be beneficial to preserve the glucosinolate content of this variety.
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Escola Superior Agrária, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: A P
authors.initials: AP
authors.lastname: Vale
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address: joanasantoscma@sapo.pt.
authors.forename: J
authors.initials: J
authors.lastname: Santos
authors.affiliation: Escola Superior Agrária, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: N V
authors.initials: NV
authors.lastname: Brito
authors.affiliation: Escola Superior Agrária, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Fernandes
authors.affiliation: CITAB/UTAD-Centre for the Research and Technology for Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Rosa
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: M Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
mesh_terms: D016588:Anticarcinogenic Agents / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D001937:Brassica / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D003624:Darkness; D005961:Glucosinolates / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D007096:Imidoesters; D010091:Oximes; D036226:Seedlings / Q000737:chemistry; D013454:Sulfoxides
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D013454:Sulfoxides; C457341:glucoiberin; C010330:sinigrin; C009048:progoitrin; D006026:Glycoside Hydrolases; C022739:thioglucosidase; C119494:glucoraphanin
keywords: Brassica sprouts; Glucosinolates; Myrosinase activity; Sprouting