title: In vitro comparative survey of cell adhesion and proliferation of human induced pluripotent stem cells on surfaces of polymeric electrospun nanofibrous and solution-cast film scaffolds.
abstract: Extracellular matrix (ECM) components play a critical role in regulating cell behaviors. Interactions between ECM components and cells are important in various biological processes, including cell attachment, survival, morphogenesis, spreading, proliferation, and gene expression. In this study the in vitro responses of human induced pluripotent stem cells (hiPSCs) on polycaprolactone (PCL) electrospun nanofibrous scaffold were reported in comparison with those of the cells on corresponding solution-cast film scaffold. Our results demonstrated that the nanofibrous scaffold showed better support for the attachment and proliferation of hiPSCs than their corresponding film scaffold. Consequently, we emphasize that hiPSCs can sense the physical properties and chemical composition of the materials and regulate their behaviors accordingly.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hoveizi
authors.affiliation: Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Ebrahimi-Barough
authors.affiliation: Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.|Advanced Medical Science and Technologies Association, Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shima
authors.initials: S
authors.lastname: Tavakol
authors.affiliation: Department of Cell and Molecular Biology, Biological Science Faculty, Kharazmi University (TMU), Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D066298:In Vitro Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D012333:RNA, Messenger; C016240:polycaprolactone
keywords: adhesion; electrospinning; hiPSCs; proliferation; scaffold