title: Microfluidic synthesis of rigid nanovesicles for hydrophilic reagents delivery.
abstract: We present a hollow-structured rigid nanovesicle (RNV) fabricated by a multi-stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic-co-glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) simulations. The entrapment efficiency of hydrophilic reagents such as calcein, rhodamine B and siRNA inside the hollow water core of RNV is ≈90 %. In comparison with the combination of free Dox and siRNA, RNV that co-encapsulate siRNA and doxorubicin (Dox) reveals a significantly enhanced anti-tumor effect for a multi-drug resistant tumor model.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 ZhongGuanCun BeiYiTiao, Beijing, 100190 (P. R. China).
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.forename: Jiuling
authors.initials: J
authors.lastname: Wang
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Xinghua
authors.initials: X
authors.lastname: Shi
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D003198:Computer Simulation; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D004339:Drug Compounding; D005452:Fluoresceins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D019344:Lactic Acid; D044085:Microfluidics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D034741:RNA, Small Interfering / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005452:Fluoresceins; D007202:Indicators and Reagents; D034741:RNA, Small Interfering; D012235:Rhodamines; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004317:Doxorubicin; C029773:rhodamine B; C007740:fluorexon
keywords: drug delivery; hydrophilic reagents; microfluidics; nanoparticles; vesicles