title: An alternative laboratory designed to address ethical concerns associated with traditional TAS2R38 student genotyping.
abstract: The TAS2R38 alleles that code for the PAV/AVI T2R38 proteins have long been viewed as benign taste receptor variants. However, recent studies have demonstrated an expanding and medically relevant role for TAS2R38. The AVI variant of T2R38 is associated with an increased risk of both colorectal cancer and Pseudomonas aeruginosa-associated sinus infection and T2R38 variants have been implicated in off-target drug responses. To address ethical concerns associated with continued student TAS2R38 gene testing, we developed an alternative to the traditional laboratory genotyping exercise. Instead of determining their own genotype, introductory level students isolated plasmid DNA containing a section of the human TAS2R38 gene from Escherichia coli. Following PCR-mediated amplification of a section of the TAS2R38 gene spanning the SNP at position 785, students determined their assigned genotype by restriction enzyme digestion and agarose gel electrophoresis. Using the course wide genotype and phenotype data, students found that there was an association between TAS2R38 genotype and the age of persistent P. aeruginosa acquisition in cystic fibrosis "patients." Assessment data demonstrated that students taking part in this new TAS2R38 laboratory activity made clear learning gains.
authors.affiliation: Department of the History of Science, Harvard University, Cambridge, Massachusetts, 02138.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: LaBonte
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Beers
mesh_terms: D026688:Bioethical Issues; D015179:Colorectal Neoplasms / Q000235:genetics; D003550:Cystic Fibrosis / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005826:Genetics, Medical / Q000193:education / Q000941:ethics; D060005:Genotyping Techniques / Q000941:ethics; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011552:Pseudomonas Infections / Q000235:genetics; D011550:Pseudomonas aeruginosa; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012852:Sinusitis / Q000235:genetics; D000098706:Taste Receptors, Type 2
chemical_list: D043562:Receptors, G-Protein-Coupled; D000098706:Taste Receptors, Type 2
keywords: SNP; TAS2R38; ethics; genotyping