title: Analysis of ethyl glucuronide and ethyl sulfate using aqueous normal-phase chromatography with mass spectrometry.
abstract: The use of aqueous normal-phase chromatography is explored as a possible format for the analysis of the forensically significant compounds ethyl glucuronide and ethyl sulfate. Standard solutions of the two compounds are used to verify the retention capabilities of two stationary phases (diamond hydride and undecanoic acid). These results are then compared to data obtained on hair extracts to determine if any matrix effects exist with respect to both retention and peak shape. The undecanoic stationary phase is used for the establishment of calibration curves for quantitative analysis. These curves are utilized to determine the concentration of ethyl glucuronide in several hair samples tested.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Dang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D013463:Sulfuric Acid Esters / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D005965:Glucuronates; D013463:Sulfuric Acid Esters; D014867:Water; C093924:ethyl glucuronide; C011612:diethyl sulfate
keywords: Alcohol markers; Hair; Silica hydride; Undecenanoic acid stationary phase