title: Mitigated reactive oxygen species generation leads to an improvement of cell proliferation on poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] functionalized polydimethylsiloxane surfaces.
abstract: In vitro cell-based analysis is strongly affected by material's surface chemical properties. The cell spreading, migration, and proliferation on a substrate surface are initiated and controlled by successful adhesion, particularly for anchor-dependent cells. Unfortunately, polydimethylsiloxane (PDMS), one of the most used polymeric materials for construction of microfluidic and miniaturized biomedical analytic devices, is not a cell-friendly surface because of its inherent hydrophobic property. Herein, a poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] (poly(GMA-co-pEGMA)) polymer brush was synthesized on a PDMS surface through a surface-initiated atom-transfer radical polymerization method. Contact angle and Fourier transform infrared characterization show that the poly (GMA-co-pEGMA) polymer brush functionalization can increase wettability of PDMS and introduce epoxy, hydroxyl, and ether groups into PDMS surface. In vitro cell growth assay demonstrates that cell adhesion and proliferation on poly(GMA-co-pEGMA) polymer brush-functionalized PDMS (poly(GMA-co-pEGMA)@PDMS) are better than on pristine PDMS. Additionally, immobilization of collagen type I (CI) and fibronectin (FN) on poly(GMA-co-pEGMA)@PDMS is better than direct coating of CI and FN on pristine PDMS to promote cell adhesion. Furthermore, increased intracellular reactive oxygen species and cell mitochondrial membrane depolarization, two indicators of cell oxidative stress, are observed from cells growing on pristine PDMS, but not from those on poly(GMA-co-pEGMA)@PDMS. Collectively, we demonstrate that poly(GMA-co-pEGMA) functionalization can enhance cell adhesion and proliferation on PDMS, and thus can be potentially used for microfluidic cell assay devices for cellular physiology study or drug screening.
authors.affiliation: Faculty of Materials & Energy, Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, China.|Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing, 400715, China.|Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases, Chongqing, 400715, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Faculty of Materials & Energy, Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, China.|Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing, 400715, China.|Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases, Chongqing, 400715, China.
authors.forename: ZhuanZhuan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Faculty of Materials & Energy, Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, China.|Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing, 400715, China.|Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases, Chongqing, 400715, China.
authors.forename: LiXia
authors.initials: L
authors.lastname: Gao
authors.affiliation: Faculty of Materials & Energy, Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, China.|Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing, 400715, China.|Chongqing Engineering Research Center for Rapid Diagnosis of Fatal Diseases, Chongqing, 400715, China.
authors.forename: ChangMing
authors.initials: C
authors.lastname: Li
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins; D008422:Materials Testing; D053078:Membrane Potential, Mitochondrial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011109:Polymethacrylic Acids / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D059010:Single-Cell Analysis
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D055767:Immobilized Proteins; D011109:Polymethacrylic Acids; D017382:Reactive Oxygen Species
keywords: PDMS functionalization; biocompatibility; cell adhesion and proliferation; cell oxidative stress; polymer brush