title: Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil.
abstract: Mycobacteriophage SWU1 is a newly isolated phage from soil sample collected in Sichuan province, China using Mycobacterium smegmatis mc(2)155 as host. Plaque, phage morphology and one-step growth curve were characterized. The complete genomic sequence of phage SWU1 was determined by shotgun sequencing. The ends of SWU1 were determined. Structural proteins of SWU1 were analyzed by NanoLC-ESI-MS/MS. Seven ORFs were identified as structural protein encoded by SWU1 genome. The genetic basis underlying the SWU1 plaque was explored using comparative genomics. Prophages homologous to SWU1 were identified in two pathogens, Segniliparus rugosus ATCC BAA-974 and Mycobacterium rhodesiae JS60. Genus Segniliparus is a member of the order Corynebacteriales. To our knowledge, this is the first report of Mycobacterium prophages in different genera.
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China; School of Biological Science and Technology, University of Jinan, Shandong 250022, China. Electronic address: fxysnd@126.com.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Fan
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: 519465867@qq.com.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Yan
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: xielongxiang123@126.com.
authors.forename: Longxiang
authors.initials: L
authors.lastname: Xie
authors.affiliation: Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA. Electronic address: lzeng@tamu.edu.
authors.forename: Lanying
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA. Electronic address: ryland@tamu.edu.
authors.forename: Ryland F
authors.initials: RF
authors.lastname: Young
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: georgex@swu.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000235:genetics*; D017353:Gene Deletion; D016679:Genome, Viral / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009160:Mycobacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D040901:Proteomics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D053719:Tandem Mass Spectrometry; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins
keywords: Electron microscopy; Genome; Mycobacteriophage; Plaque; Proteome