title: Comparison of four molecular methods to type Salmonella Enteritidis strains.
abstract: This study compared the pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), multilocus variable-number of tanden-repeat analysis (MLVA), and multilocus sequence typing (MLST) methods for typing 188 Salmonella Enteritidis strains from different sources isolated over a 24-year period in Brazil. PFGE and ERIC-PCR were more efficient than MLVA for subtyping the strains. However, MLVA provided additional epidemiological information for those strains. In addition, MLST showed the Brazilian strains as belonging to the main clonal complex of S. Enteritidis, CC11, and provided the first report of two new STs in the S. enterica database but could not properly subtype the strains. Our results showed that the use of PFGE or ERIC-PCR together with MLVA is suitable to efficiently subtype S. Enteritidis strains and provide important epidemiological information.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Campioni
authors.forename: André
authors.initials: A
authors.lastname: Pitondo-Silva
authors.forename: Alzira M M
authors.initials: AM
authors.lastname: Bergamini
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002645:Chickens / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D058872:Epidemics; D005516:Food Microbiology; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D058885:Multilocus Sequence Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
keywords: ERIC-PCR; MLST; MLVA; PFGE; Salmonella Enteritidis; molecular epidemiology