title: Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis.
abstract: We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%).
authors.affiliation: Laboratoire de Parasitologie et Mycologie Médicale, Les Hôpitaux Universitaires de Strasbourg, Université de Strasbourg, Strasbourg, France Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Centre Hospitalier Universitaire of Montpellier, Montpellier, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Filisetti
authors.affiliation: Laboratoire de Parasitologie Mycologie, Hôpital Cochin, Hôpitaux Universitaires Paris Centre AP-HP, Université Paris Descartes, Paris, France Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Centre Hospitalier Universitaire of Montpellier, Montpellier, France helene.yera@cch.aphp.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Yera
authors.affiliation: Laboratoire de Parasitologie et Mycologie Médicale, Les Hôpitaux Universitaires de Strasbourg, Université de Strasbourg, Strasbourg, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Villard
authors.affiliation: Service de Pédiatrie 2, Les Hôpitaux Universitaires de Strasbourg, Université de Strasbourg, Strasbourg, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Escande
authors.affiliation: Gynécologie-Obstérique, Centre Hospitalier de Marne-La-Vallée, Jossigny, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Wafo
authors.affiliation: Gynécologie-Obstérique, Université Lille Nord de France (UDSL), CHRU de Lille, Lille, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Houfflin-Debarge
authors.affiliation: Laboratoire de Parasitologie Mycologie, EA4547-BDEEP, CIIL, Faculté de Médecine, Université Lille Nord de France (UDSL), CHRU de Lille, Lille, France Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Centre Hospitalier Universitaire of Montpellier, Montpellier, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Delhaes
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU (University Hospital Centre) of Montpellier, UMR MIVEGEC CNRS 5290/IRD 224/University of Montpellier, Montpellier, France Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Centre Hospitalier Universitaire of Montpellier, Montpellier, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bastien
mesh_terms: D000653:Amniotic Fluid / Q000469:parasitology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013997:Time Factors; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology