title: A Sertoli cell-specific connexin43 knockout leads to altered interstitial connexin expression and increased Leydig cell numbers.
abstract: The Sertoli cell (SC)-specific knockout (KO) of connexin43 (Cx43) results in spermatogenic arrest at the level of spermatogonia and/or SC-only syndrome. Histology of the interstitial compartment suggests Leydig cell (LC) hyperplasia. Our aim has been to investigate possible effects of the SC-specific KO of Cx43 (SCCx43KO) on interstitial LC. We therefore counted LC via the optical dissector method (per microliter of testicular tissue and per testis) and found LC to be significantly increased in SCCx43KO(-/-) compared with wild-type mice. Semiquantitative western blot together with Cx43 and 3β-hydroxysteroid dehydrogenase immunohistochemistry showed that Cx43 protein was significantly reduced and barely detectable in LC in adult SCCx43KO(-/-) mice. This reduction of Cx43 protein was accompanied by a reduction of Cx43 mRNA as analyzed by laser-assisted microdissection of interstitial cells and subsequent quantitative real-time polymerase chain reaction (PCR). Interestingly, Cx45, another recently detected connexin in LC, was also downregulated. Preliminary qualitative data of LC differentiation markers (Thb2, Hsd3b6) and a steroidogenic marker (Hsd17b3) obtained by reverse transcription plus PCR revealed no obvious differences. Thus, the loss of Cx43 in SC also provokes the downregulation of connexins in interstitial LC at the transcriptional and translational levels. Moreover, SCCx43KO leads to alterations in LC numbers. Despite these alterations, steroidogenesis seems not to be impaired. Further studies, including ultrastructural analysis of the tissue as well as quantitative examination of additional LC markers and testosterone, and functional in vitro experiments, should provide more information about LC differentiation and function in SCCx43KO(-/-) mice.
authors.affiliation: Institute of Anatomy, University of Veterinary Medicine Hannover, Foundation, 30173, Hannover, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Noelke
authors.forename: Joachim
authors.initials: J
authors.lastname: Wistuba
authors.forename: Oliver S
authors.initials: OS
authors.lastname: Damm
authors.forename: Daniela
authors.initials: D
authors.lastname: Fietz
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gerber
authors.forename: Marion
authors.initials: M
authors.lastname: Gaehle
authors.forename: Ralph
authors.initials: R
authors.lastname: Brehm
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D018031:Connexin 43 / Q000032:analysis / Q000235:genetics*; D017630:Connexins / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D007985:Leydig Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism
chemical_list: D018031:Connexin 43; D017630:Connexins; C543076:GJA1 protein, mouse; D012333:RNA, Messenger; D013256:Steroids; D015096:3-Hydroxysteroid Dehydrogenases