title: Light-cleavable rapamycin dimer as an optical trigger for protein dimerization.
abstract: Rapamycin-induced protein heterodimerization of FKBP12 and FRB is one of the most commonly employed switches to conditionally control biological processes. We developed an optically activated rapamycin dimer that does not induce FKBP12-FRB dimerization until exposed to light, and applied it to control kinase, protease, and recombinase function.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA. deiters@pitt.edu.
authors.forename: Kalyn A
authors.initials: KA
authors.lastname: Brown
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: David
authors.initials: D
authors.lastname: Shirvanyants
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Subhas
authors.initials: S
authors.lastname: Samanta
authors.forename: Pavan K
authors.initials: PK
authors.lastname: Mantravadi
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Dokholyan
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D006801:Humans; D008968:Molecular Conformation; D055095:Optics and Photonics; D010782:Photolysis / Q000528:radiation effects*; D055503:Protein Multimerization / Q000528:radiation effects*; D020123:Sirolimus / Q000737:chemistry* / Q000528:radiation effects*; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D022061:Tacrolimus Binding Protein 1A; D020123:Sirolimus