title: Cancer cell-specific oligopeptides selected by an integrated microfluidic system from a phage display library for ovarian cancer diagnosis.
abstract: Ovarian cancer is one of the leading causes of female mortality worldwide. Unfortunately, there are currently few high-specificity candidate oligopeptide targeting agents that can be used for early diagnosis of this cancer. It has been suggested that cancer-specific oligopeptides could be screened from a phage display library. However, conventional methods are tedious, labor-intensive, and time consuming. Therefore, a novel, integrated microfluidic system was developed to automate the entire screening process for ovarian cancer cell-specific oligopeptides. An oligopeptide screened with microfluidic chip-based technique was demonstrated to have high affinity to ovarian cancer cells and demonstrated relatively low binding to other cancer cells, indicating a high specificity. Furthermore, the developed method consumed relatively low volumes of samples and reagents; only 70 μL of reactant was used within the whole experimental process. Each panning process was also significantly shortened to only 7.5 hours. Therefore, the screened oligopeptide could be used to isolate ovarian cancer cells in a rapid manner, thus greatly expediting the diagnosis and its application as oligopeptide targeting agent for theranostics of this cancer.
authors.affiliation: 1. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Wang
authors.affiliation: 1. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Chen-Hsun
authors.initials: CH
authors.lastname: Weng
authors.affiliation: 1. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Yu-Jui
authors.initials: YJ
authors.lastname: Che
authors.affiliation: 4. Nanomedicine Program and Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Kuan
authors.initials: K
authors.lastname: Wang
authors.affiliation: 1. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan ; 2. Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan ; 3. Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Gwo-Bin
authors.initials: GB
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D003955:Diagnostic Tests, Routine / Q000379:methods; D005260:Female; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D009842:Oligopeptides / Q000302:isolation & purification*; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D009842:Oligopeptides; D019151:Peptide Library
keywords: microfluidic chip, oligopeptide targeting agent, ovarian cancer; phage display library