title: Survival of Salmonella enterica serovar infantis on and within stored table eggs.
abstract: Contaminated table eggs are considered a primary source of foodborne salmonellosis globally. Recently, a single clone of Salmonella enterica serovar Infantis emerged in Israel and became the predominant serovar isolated in poultry. This clone is currently the most prevalent strain in poultry and is the leading cause of salmonellosis in humans. Because little is known regarding the potential transmission of this strain from contaminated eggs to humans, the objective of this study was to evaluate the ability of Salmonella Infantis to survive on the eggshell or within the egg during cold storage or at room temperature. Salmonella cells (5.7 log CFU per egg) were inoculated on the surface of 120 intact eggs or injected into the egg yolk (3.7 log CFU per egg) of another 120 eggs. Half of the eggs were stored at 5.5 ± 0.3°C and half at room temperature (25.5 ± 0.1°C) for up to 10 weeks. At both temperatures, the number of Salmonella cells on the shell declined by 2 log up to 4 weeks and remained constant thereafter. Yolk-inoculated Salmonella counts at cold storage declined by 1 log up to 4 weeks and remained constant, while room-temperature storage supported the growth of the pathogen to a level of 8 log CFU/ml of total egg content, as early as 4 weeks postinoculation. Examination of egg content following surface inoculation revealed the presence of Salmonella in a portion of the eggs at both temperatures up to 10 weeks, suggesting that this strain can also penetrate through the shell and survive within the egg. These findings imply that Salmonella enterica serovar Infantis is capable of survival both on the exterior and interior of table eggs and even multiply inside the egg at room temperature. Our findings support the need for prompt refrigeration to prevent Salmonella multiplication during storage of eggs at room temperature.
authors.affiliation: Division of Avian and Fish Diseases, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel. avishailublin@yahoo.com.
authors.forename: Avishai
authors.initials: A
authors.lastname: Lublin
authors.affiliation: The Laboratory of Food Microbiology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel.
authors.forename: Ilana
authors.initials: I
authors.lastname: Maler
authors.affiliation: Division of Avian and Fish Diseases, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel.
authors.forename: Sara
authors.initials: S
authors.lastname: Mechani
authors.affiliation: Department of Food Quality and Safety, Institute of Postharvest Technology and Food Science, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel.
authors.forename: Riky
authors.initials: R
authors.lastname: Pinto
authors.affiliation: Department of Food Quality and Safety, Institute of Postharvest Technology and Food Science, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel.
authors.forename: Shlomo
authors.initials: S
authors.lastname: Sela-Saldinger
mesh_terms: D000818:Animals; D002645:Chickens; D015169:Colony Count, Microbial; D004528:Egg Shell / Q000382:microbiology*; D004530:Egg Yolk / Q000382:microbiology; D004531:Eggs / Q000382:microbiology*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D061353:Food Storage / Q000379:methods; D006801:Humans; D012034:Refrigeration; D019779:Salmonella enterica / Q000145:classification / Q000302:isolation & purification*; D065288:Serogroup; D013696:Temperature