title: BODIPY-based fluorometric sensor for the simultaneous determination of Cys, Hcy, and GSH in human serum.
abstract: Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are interconnected and play essential roles for regulating the redox balance of biological processes. However, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. In this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (BODIPY)-based fluorometric sensor. At a specified period of time, after reacting with excess sensor, Hcy and GSH form predominantly sulfur-substituted BODIPY, while Cys generates sulfur-amino-diBODIPY due to a fast substitution-rearrangement-substitution reaction. A significant difference in polarities of these respective major products simplifies their separation by TLC, thus leading to the simultaneous determination of Cys, Hcy, and GSH readily. The sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (HPLC).
authors.affiliation: †Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.|§University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Jia
authors.affiliation: ‡Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100191, P. R. China.
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Niu
authors.affiliation: †Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: ‡Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100191, P. R. China.
authors.forename: Qing-Zheng
authors.initials: QZ
authors.lastname: Yang
authors.affiliation: ‡Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100191, P. R. China.
authors.forename: Chen-Ho
authors.initials: CH
authors.lastname: Tung
authors.affiliation: †Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Guan
authors.affiliation: †Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001896:Boron Compounds / Q000737:chemistry*; D045424:Complex Mixtures / Q000097:blood; D003545:Cysteine / Q000097:blood*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005470:Fluorometry / Q000295:instrumentation*; D005978:Glutathione / Q000097:blood*; D006710:Homocysteine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D045424:Complex Mixtures; D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine
keywords: BODIPY; cysteine; glutathione; homocysteine; serum