title: Aptamers in diagnostics and treatment of viral infections.
abstract: Aptamers are in vitro selected DNA or RNA molecules that are capable of binding a wide range of nucleic and non-nucleic acid molecules with high affinity and specificity. They have been conducted through the process known as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). It serves to reach specificity and considerable affinity to target molecules, including those of viral origin, both proteins and nucleic acids. Properties of aptamers allow detecting virus infected cells or viruses themselves and make them competitive to monoclonal antibodies. Specific aptamers can be used to interfere in each stage of the viral replication cycle and also inhibit its penetration into cells. Many current studies have reported possible application of aptamers as a treatment or diagnostic tool in viral infections, e.g., HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus), HCV (Hepatitis C Virus), SARS (Severe Acute Respiratory Syndrome), H5N1 avian influenza and recently spread Ebola. This review presents current developments of using aptamers in the diagnostics and treatment of viral diseases.
authors.affiliation: Department of Gene Therapy, Faculty of Medicine, Nicolaus Copernicus University in Toruń, M. Curie-Sklodowskiej 9 St., 85-094 Bydgoszcz, Poland. tomasz_wandtke@wp.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wandtke
authors.affiliation: Department of Gene Therapy, Faculty of Medicine, Nicolaus Copernicus University in Toruń, M. Curie-Sklodowskiej 9 St., 85-094 Bydgoszcz, Poland. wozniak.jkw@gmail.com.
authors.forename: Joanna
authors.initials: J
authors.lastname: Woźniak
authors.affiliation: Department of Gene Therapy, Faculty of Medicine, Nicolaus Copernicus University in Toruń, M. Curie-Sklodowskiej 9 St., 85-094 Bydgoszcz, Poland. mpkopins@hotmail.com.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kopiński
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000494:pharmacology / Q000627:therapeutic use*; D019142:Hemorrhagic Fever, Ebola / Q000628:therapy; D006801:Humans; D052156:SELEX Aptamer Technique; D014777:Virus Diseases / Q000175:diagnosis* / Q000628:therapy*; D018406:Virus Physiological Phenomena / Q000187:drug effects
chemical_list: D052157:Aptamers, Nucleotide