title: Exploring Actinobacteria assemblages in coastal marine sediments under contrasted Human influences in the West Istria Sea, Croatia.
abstract: The exploration of marine Actinobacteria has as major challenge to answer basic questions of microbial ecology that, in turn, will provide useful information to exploit Actinobacteria metabolisms in biotechnological processes. The ecological functions performed by Actinobacteria in marine sediments are still unclear and belongs to the most burning basic questions. The comparison of Actinobacteria communities inhabiting marine sediments that are under the influence of different contamination types will provide valuable information in the adaptation capacities of Actinobacteria to colonize specific ecological niche. In the present study, the characterization of different Actinobacteria assemblages according to contamination type revealed the ecological importance of Actinobacteria for maintaining both general biogeochemical functions through a "core" Actinobacteria community and specific roles associated with the presence of contaminants. Indeed, the results allowed to distinguish Actinobacteria genera and species operational taxonomic units (OTUs) able to cope with the presence of either (i) As, (ii) metals Ni, Fe, V, Cr, and Mn, or (iii) polycyclic aromatic hydrocarbons (PAHs) and toxic metals (Hg, Cd, Cu, Pb, and Zn). Such observations highlighted the metabolic capacities of Actinobacteria and their potential that should be taken into consideration and advantage during the implementation of bioremediation processes in marine ecosystems.
authors.affiliation: Equipe Environnement et Microbiologie, MELODY group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France. robert.duran@univ-pau.fr.|Université de Pau et des Pays de l'Adour, Bâtiment IBEAS, BP1155, 64013, Pau Cedex, France. robert.duran@univ-pau.fr.
authors.forename: Robert
authors.initials: R
authors.lastname: Duran
authors.affiliation: Division of Molecular Biology, Institute Ruđer Bošković, Bijenička 54, 10000, Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Bielen
authors.affiliation: Division of Molecular Biology, Institute Ruđer Bošković, Bijenička 54, 10000, Zagreb, Croatia.
authors.forename: Tina
authors.initials: T
authors.lastname: Paradžik
authors.affiliation: Equipe Environnement et Microbiologie, MELODY group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Gassie
authors.affiliation: Juraj Dobrila University of Pula, Zagrebačka 30, 52100, Pula, Croatia.
authors.forename: Emina
authors.initials: E
authors.lastname: Pustijanac
authors.affiliation: Equipe Environnement et Microbiologie, MELODY group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Cagnon
authors.affiliation: Center for Marine Research, Ruđer Bošković Institute, Giordano Paliaga 5, 52210, Rovinj, Croatia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Hamer
authors.affiliation: Division of Molecular Biology, Institute Ruđer Bošković, Bijenička 54, 10000, Zagreb, Croatia.
authors.forename: Dušica
authors.initials: D
authors.lastname: Vujaklija
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification*; D001151:Arsenic / Q000032:analysis; D017523:Croatia; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000382:microbiology*; D019216:Metals, Heavy / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D001151:Arsenic
keywords: Actinobacteria; Bacteria diversity; Co-occurrence network; Community structure; Hydrocarbon contamination; Marine coastal sediments; Metal contamination; T-RFLP