title: Individual surface-engineered microorganisms as robust Pickering interfacial biocatalysts for resistance-minimized phase-transfer bioconversion.
abstract: A powerful strategy for long-term and diffusional-resistance-minimized whole-cell biocatalysis in biphasic systems is reported where individually encapsulated bacteria are employed as robust and recyclable Pickering interfacial biocatalysts. By individually immobilizing bacterial cells and optimizing the hydrophobic/hydrophilic balance of the encapsulating magnetic mineral shells, the encased bacteria became interfacially active and locate at the Pickering emulsion interfaces, leading to dramatically enhanced bioconversion performances by minimizing internal and external diffusional resistances. Moreover, in situ product separation and biocatalyst recovery was readily achieved using a remote magnetic field. Importantly, the mineral shell effectively protected the entire cell from long-term organic-solvent stress, as shown by the reusability of the biocatalysts for up to 30 cycles, while retaining high stereoselective catalytic activities, cell viabilities, and proliferative abilities.
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Graduate School of the Chinese Academy of Sciences, Changchun, Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022 (China).
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Chen
authors.forename: Haiwei
authors.initials: H
authors.lastname: Ji
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Enguo
authors.initials: E
authors.lastname: Ju
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D042481:Alcaligenes faecalis / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D055162:Biocatalysis; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D005452:Fluoresceins / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D008280:Magnetics; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry
chemical_list: D004655:Emulsions; D005452:Fluoresceins; C018506:diacetylfluorescein
keywords: biocatalysis; cell encapsulation; interfaces; nanostructures; phase transfer