title: Overexpression of Hiwi Inhibits the Cell Growth of Chronic Myeloid Leukemia K562 Cells and Enhances Their Chemosensitivity to Daunomycin.
abstract: Chronic myeloid leukemia (CML) is a clonal disorder characterized by excessive accumulation of myeloid cells in the peripheral blood. In the present study, to investigate the role of Hiwi in leukemogenesis, lentivirus-mediated Hiwi overexpression was performed in a CML cell line, K562 cells. Our data revealed that Hiwi protein expression was undetectable in K562 cells, and its overexpression suppressed cell proliferation, induced cell cycle arrest at G0/G1 and G2/M phases, and promoted apoptosis in K562 cells in vitro. Expression of anti-apoptotic protein, Bcl-2, was decreased in cells expressing Hiwi, whereas that of pro-apoptotic proteins, Bax, activated caspase-3, -9, and cleaved poly (ADP-ribose) polymerase were increased. Additionally, Hiwi upregulation enhanced the chemosensitivity of CML cells to daunomycin. Our study illustrates that expression deletion of Hiwi may be involved in the pathogenesis of human CML and suggests a possible role of Hiwi in regulating the cell growth, cell cycle, and apoptosis of CML cells in vitro.
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Bian
authors.affiliation: Department of Oncology, Affiliated Hospital of Chengde Medical College, Chengde, 067000, Hebei, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, 130021, Jilin, People's Republic of China. liuzilinglzl@126.com.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003630:Daunorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*
chemical_list: D000903:Antibiotics, Antineoplastic; D051017:Apoptosis Regulatory Proteins; D060565:Argonaute Proteins; C421088:PIWIL1 protein, human; D003630:Daunorubicin
keywords: Cell apoptosis; Cell proliferation; Chemotherapy; Chronic myeloid leukemia; Hiwi