title: A cell-signaling network temporally resolves specific versus promiscuous phosphorylation.
abstract: If specific and functional kinase- or phosphatase-substrate interactions are optimized for binding compared to promiscuous interactions, then changes in phosphorylation should occur faster on functional versus promiscuous substrates. To test this hypothesis, we designed a high temporal resolution global phosphoproteomics protocol to study the high-osmolarity glycerol (HOG) response in the budding yeast Saccharomyces cerevisiae. The method provides accurate, stimulus-specific measurement of phosphoproteome changes, quantitative analysis of phosphodynamics at sub-minute temporal resolution, and detection of more phosphosites. Rates of evolution of dynamic phosphosites were comparable to those of known functional phosphosites and significantly lower than static or longer-time-frame dynamic phosphosites. Kinetic profile analyses indicated that putatively functional kinase- or phosphatase-substrate interactions occur more rapidly, within 60 s, than promiscuous interactions. Finally, we report many changes in phosphorylation of proteins implicated in cytoskeletal and mitotic spindle dynamics that may underlie regulation of cell cycle and morphogenesis.
authors.affiliation: Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada; Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kanshin
authors.affiliation: Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada.
authors.forename: Louis-Philippe
authors.initials: LP
authors.lastname: Bergeron-Sandoval
authors.affiliation: Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada.
authors.forename: S Sinan
authors.initials: SS
authors.lastname: Isik
authors.affiliation: Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada; Département de Chimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada; Institute for Research in Immunology and Cancer, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada. Electronic address: pierre.thibault@umontreal.ca.
authors.forename: Pierre
authors.initials: P
authors.lastname: Thibault
authors.affiliation: Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada; Centre Robert-Cedergren, Bio-Informatique et Génomique, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, QC H3C 3J7, Canada. Electronic address: stephen.michnick@umontreal.ca.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Michnick
mesh_terms: D000199:Actins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D008870:Microtubules / Q000378:metabolism; D009994:Osmolar Concentration; D010748:Phosphopeptides / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D010748:Phosphopeptides; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases; D005990:Glycerol