title: [A multienzyme bioreactor based on a chitinase complex].
abstract: A heterogeneous biocatalyst containing a complex of chitinolytic enzymes isolated from the culture medium of bacteria Clostridium paraputrificum on the surface of macroporous monolithic minidisc was obtained. The complex of chitinolytic enzymes was immobilized on the polymer matrix using a multistep method involving the introduction of an intermediate macromolecular spacer. The endochitinase and N-acetylglucosaminidase activity of the heterogeneous biocatalyst was studied.
authors.forename: E G
authors.initials: EG
authors.lastname: Vlakh
authors.forename: E A
authors.initials: EA
authors.lastname: Ponomareva
authors.forename: T B
authors.initials: TB
authors.lastname: Tennikova
mesh_terms: D000118:Acetylglucosaminidase / Q000737:chemistry*; D001426:Bacterial Proteins; D019149:Bioreactors; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry; D002688:Chitinases / Q000737:chemistry*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid / Q000295:instrumentation; D003013:Clostridium / Q000737:chemistry* / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D002686:Chitin; D002688:Chitinases; D000118:Acetylglucosaminidase