title: A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples.
abstract: Foodborne disease is a major public health threat worldwide. Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture or molecular-based methods are time consuming and usually take a few hours to days to complete. In response to the demand for rapid on line or on site detection of pathogens, in this study, we describe for the first time an eight-chamber lab-on-a-chip (LOC) system with integrated magnetic bead-based sample preparation and loop-mediated isothermal amplification (LAMP) for rapid and quantitative detection of Salmonella spp. in food samples. The whole diagnostic procedures including DNA isolation, isothermal amplification, and real-time detection were accomplished in a single chamber. Up to eight samples could be handled simultaneously and the system was capable to detect Salmonella at concentration of 50 cells per test within 40 min. The simple design, together with high level of integration, isothermal amplification, and quantitative analysis of multiple samples in short time, will greatly enhance the practical applicability of the LOC system for rapid on-site screening of Salmonella for applications in food safety control, environmental surveillance, and clinical diagnostics.
authors.affiliation: Department of Micro and Nanotechnology (DTU-Nanotech), Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Than Linh
authors.initials: TL
authors.lastname: Quyen
authors.forename: Tran Quang
authors.initials: TQ
authors.lastname: Hung
authors.forename: Wai Hoe
authors.initials: WH
authors.lastname: Chin
authors.forename: Anders
authors.initials: A
authors.lastname: Wolff
authors.forename: Dang Duong
authors.initials: DD
authors.lastname: Bang
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation*; D004396:Coloring Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D007364:Intercalating Agents / Q000494:pharmacology; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D019779:Salmonella enterica / Q000235:genetics* / Q000302:isolation & purification*; D018511:Systems Integration; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial; D007364:Intercalating Agents