title: Actual Isothermal Effects of Water-Filtered Infrared A-Irradiation.
abstract: In this study, the athermal effects of water-filtered infrared A (wIRA)-irradiation (780-1400 nm) on human dermal fibroblasts were investigated. For this purpose, cells were exposed to wIRA-irradiation (178 mW cm(-2) for 1 h), while a sophisticated experimental setup prevented warming of the samples exceeding 0.1°C. The investigated parameters were the formation of reactive oxygen species (ROS), mitochondrial membrane potential and superoxide release, protein oxidation, proliferation rate, as well as intracellular Ca(2+) -release in single cells, most of them quantified via fluorescence microscopy and fluorimetric techniques. The existence of actual athermal wIRA-effects is still intensively discussed, since their detection requires a careful experimental setup and both efficient and powerful temperature regulation of the exposed samples. Here, we can definitively show that some of the supposed athermal wIRA-effects may be rather artifacts, since wIRA did not reveal any impact on the above mentioned parameters-as long as the temperature of the exposed cells was carefully maintained. Though, we were able to identify an athermal DNA-protective wIRA-effect, since the induced DNA damage (quantified via 8-Oxo-G-formation) was significantly decreased after a subsequent UVB-exposure. These results suggest that many of the supposed athermal wIRA-effects can be induced by pure warming of the samples, independent from any wIRA-irradiation.
authors.affiliation: Department for "Molecular Toxicology", German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.|Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Höhn
authors.affiliation: Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Hartmann
authors.affiliation: Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Gebhart
authors.affiliation: Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Sonntag
authors.affiliation: Department for "Molecular Toxicology", German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.|Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
authors.affiliation: Department for "Molecular Toxicology", German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.|Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Jung
mesh_terms: D002118:Calcium / Q000378:metabolism; D005374:Filtration; D007259:Infrared Rays; D017382:Reactive Oxygen Species / Q000378:metabolism; D014867:Water
chemical_list: D017382:Reactive Oxygen Species; D014867:Water; D002118:Calcium