title: High repetition rate atmospheric pressure matrix-assisted laser desorption/ionization in combination with liquid matrices.
abstract: One major drawback of matrix-assisted laser desorption/ionization (MALDI) is still the relatively poor pulse-to-pulse reproducibility of the signal intensity. This problem, caused by insufficient homogeneity in the matrix/analyte co-crystallization, is usually circumvented by averaging the detected ion intensity over several shots. However, during the consecutive laser pulses, the applied matrix gets depleted and only a number of subsequent experiments can be done on the same sample spot. In order to achieve the desired long-term stability in combination with a sufficient pulse-to-pulse reproducibility, recently liquid MALDI matrices have been introduced. This contribution demonstrates the promising combination of liquid matrices with high repetition rate lasers for atmospheric pressure MALDI (AP-MALDI). To demonstrate the robustness of the new approach, two different kinds of liquid matrices were used in combination with both a typical flashlamp pumped 15 Hz laser and a diode pumped solid state laser operated at 5 kHz. The latter showed a stable ion signal over more than 3,500,000 consecutive laser pulses.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bierstedt
authors.forename: Arne
authors.initials: A
authors.lastname: Stindt
authors.forename: Carsten
authors.initials: C
authors.lastname: Warschat
authors.forename: Ulrich
authors.initials: U
authors.lastname: Panne
authors.forename: Jens
authors.initials: J
authors.lastname: Riedel