title: A β-lactamase based assay to measure surface expression of membrane proteins.
abstract: Measurement of cell surface expression is an essential part of studying membrane proteins. Traditional techniques for measuring surface expression depend on the availability of appropriate radioligands or antibodies towards extracellular epitopes of a protein of interest. The current protocol outlines the use of an assay to monitor surface expression of membrane proteins tagged with a bacterial β-lactamase in mammalian cell lines. The use of this technique allows for quick, quantitative, sensitive, and inexpensive measurement of surface expression, with the potential for high-throughput screening.
authors.affiliation: Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
authors.forename: Pieter
authors.initials: P
authors.lastname: Beerepoot
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Lam
authors.forename: Ali
authors.initials: A
authors.lastname: Salahpour
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D057075:Enzyme Assays; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D001618:beta-Lactamases