title: Direct detection, cloning and characterization of a glucoside hydrolase from forest soil.
abstract: A glucoside hydrolase gene, egl01, was cloned from the soil DNA of Changbai Mountain forest by homologous PCR amplification. The deduced sequence of 517 amino acids included a catalytic domain of glycoside hydrolase family 5 and was homologous to a putative cellulase from Bacillus licheniformis. The recombinant enzyme, Egl01, was maximally active at pH 5 and 50 °C and it was stable at pH 3-9, 4-50 °C, and also stable in the presence of metal ions, organic solvents, surfactants and salt. Its activity was above 120 % in 2-3 M NaCl/KCl and over 70 % was retained in 1-4 M NaCl/KCl for 6d. Egl01 hydrolyzed carboxymethyl cellulose, beechwood xylan, crop stalk, laminarin, filter paper, and avicel but not pNPG, indicating its broad substrate specificity. These properties make this recombinant enzyme a promising candidate for industrial applications.
authors.affiliation: School of Life Science, Northeast Normal University, Changchun, 130024, China, huam149@nenu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Hua
authors.forename: Shubo
authors.initials: S
authors.lastname: Zhao
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Dongbo
authors.initials: D
authors.lastname: Liu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Xia
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
mesh_terms: D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D065928:Forests; D006863:Hydrogen-Ion Concentration; D054892:Metagenome; D011189:Potassium Chloride / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000378:metabolism; D012987:Soil; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D020536:Enzyme Activators; D011994:Recombinant Proteins; D012987:Soil; D012965:Sodium Chloride; D011189:Potassium Chloride; D002480:Cellulase