title: Determination of kurarinone in rat plasma by UPLC-MS/MS.
abstract: A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine kurarinone in rat plasma using chlorzoxazone as the internal standard (IS). Sample preparation was accomplished through a liquid-liquid extraction procedure with ethyl acetate to 0.2 mL plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 437.0→301.2 for kurarinone and m/z 168.1→132.1 for IS. The linearity of this method was found to be within the concentration range of 20-2000 ng/mL with a lower limit of quantification of 20 ng/mL. Only 3.0 min was needed for an analytical run. The matrix effect was 94.7-107.2% for kurarinone. The intra- and inter-day precision (RSD%) were less than 8.2% and accuracy (RE%) was within ±9.0%. The recovery ranged from 77.3% to 85.6%. Kurarinone was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of kurarinone in rats.
authors.affiliation: The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China. Electronic address: lyzhangweimin@163.com.
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Zhang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Rui-fang
authors.initials: RF
authors.lastname: Li
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Jian-fei
authors.initials: JF
authors.lastname: Qiu
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Zhi-yin
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bian
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Lei
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005419:Flavonoids / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids; C411319:kurarinone
keywords: Kurarinone; Pharmacokinetics; Rat plasma; UPLC–MS/MS