title: Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells.
abstract: Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 μg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle.
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina. Electronic address: pecora.andrea@inta.gob.ar.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pecora
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
authors.forename: Darío A
authors.initials: DA
authors.lastname: Malacari
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
authors.forename: María S
authors.initials: MS
authors.lastname: Pérez Aguirreburualde
authors.affiliation: Vetanco S.A., Buenos Aires, Argentina.
authors.forename: Demian
authors.initials: D
authors.lastname: Bellido
authors.affiliation: Algenex SL, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Escribano
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
authors.forename: María J
authors.initials: MJ
authors.lastname: Dus Santos
authors.affiliation: Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
authors.forename: Andrés
authors.initials: A
authors.lastname: Wigdorovitz
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology*; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D006023:Glycoproteins / Q000276:immunology*; D006168:Guinea Pigs; D057127:Single-Chain Antibodies / Q000276:immunology*; D022223:Vaccines, Subunit
chemical_list: D006023:Glycoproteins; D057127:Single-Chain Antibodies; D022223:Vaccines, Subunit
keywords: Anticuerpo de cadena simple; Baculovirus; Bovine viral diarrhea virus; E2 glycoprotein; Glucoproteína E2; Single chain antibody; Subunit vaccine; Vacuna a subunidad; Virus de la diarrea viral bovina