title: [Oxidative stress in uveitis and its correction with superoxide dismutase antioxidative enzyme (experimental study)].
abstract: OBJECTIVE
  to study the influence of experimental uveitis on those biochemical parameters of aqueous humor that reflect inflammation acuity as well as local antioxidant and local antiproteolytic activity; to study the effect of topical superoxide dismutase (SOD) on the clinical course of uveitis and ocular metabolism.
  
  
  METHODS
  Acute uveitis was induced in rabbits by a double injection (subcutaneous and intravitreal) of normal horse serum. The following parameters of aqueous humor were measured: protein concentration, antioxidant activity, SOD activity, alpha2-macroglobulin level, total nitrates and nitrites, and leukocyte number. Clinical assessment and histopathological study were performed.
  
  
  RESULTS
  It was found that uveitis is associated with a statistically significant increase in protein concentration, leukocyte number, SOD activity, and alpha2-macroglobulin level in aqueous humor as well as a decrease in anti-hydroxyl radical activity. SOD instillations contributed to the reduction of the listed parameters and improvement of the antioxidant activity. Clinical presentations of uveitis also became less pronounced.
  
  
  CONCLUSIONS
  SOD instillations for oxidative stress correction help reduce clinical presentations of uveitis, which is confirmed by biochemical examination.
authors.forename: N B
authors.initials: NB
authors.lastname: Chesnokova
authors.forename: V V
authors.initials: VV
authors.lastname: Neroev
authors.forename: O V
authors.initials: OV
authors.lastname: Beznos
authors.forename: G A
authors.initials: GA
authors.lastname: Beĭshenova
authors.forename: I I
authors.initials: II
authors.lastname: Nikol'skaia
authors.forename: O A
authors.initials: OA
authors.lastname: Kost
authors.forename: P V
authors.initials: PV
authors.lastname: Binevskiĭ
authors.forename: A B
authors.initials: AB
authors.lastname: Shekhter
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001082:Aqueous Humor / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D003315:Cornea / Q000473:pathology; D004195:Disease Models, Animal; D018384:Oxidative Stress; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism / Q000494:pharmacology; D016896:Treatment Outcome; D014605:Uveitis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D000511:alpha-Macroglobulins / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D000511:alpha-Macroglobulins; D013482:Superoxide Dismutase