title: Deep Invasive Endometriosis Lesions of the Rectosigmoid May Be Related to Alterations in Cell Kinetics.
abstract: OBJECTIVE
  The aim of this study was to analyze cell kinetics through expression and apoptosis of topoisomerase 2-α (TOP2A), p53, and c-erb2 in rectosigmoid endometriotic lesions and in healthy endometrial tissue and to establish correlations between such findings and clinical data in patients with rectosigmoid endometriosis.
  
  
  METHODS
  Sixty patients with rectosigmoid endometriosis and 20 control women without endometriosis were included. Immunohistochemical assays were used to measure expression of TOP2A, p53, and c-erB-2. Apoptosis was quantified by directly counting the apoptotic bodies.
  
  
  RESULTS
  The number of lesions was positively correlated with expression of TOP2A in the lesion. There was also significant correlation between the lesions' size and number and cell turnover index. Apoptosis index (AI) was the same for endometriosis lesions and eutopic endometrium. Expression of TOP2A was significantly lower in the endometriosis group compared to the controls.
  
  
  CONCLUSIONS
  Changes in cell proliferation but not in the AI in rectosigmoid endometriosis are indicative of an imbalance in cell kinetics that may lead to the development of the disease.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Bassi
authors.affiliation: Department of Pathology, Perola Byington Hospital, São Paulo, Brazil.
authors.forename: Victor
authors.initials: V
authors.lastname: Arias
authors.affiliation: Department of Obstetrics and Gynecology, Samaritano Hospital, São Paulo, Brazil.
authors.forename: Nicolau
authors.initials: N
authors.lastname: D'Amico Filho
authors.affiliation: Jewish Teaching and Research Institute, Albert Einstein Hospital, São Paulo, Brazil.
authors.forename: Bárbara Yasmin
authors.initials: BY
authors.lastname: Gueuvoghlanian-Silva
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Mauricio Simoes
authors.initials: MS
authors.lastname: Abrao
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Sao Paulo, São Paulo, Brazil Jewish Teaching and Research Institute, Albert Einstein Hospital, São Paulo, Brazil sergiopodgaec@me.com.
authors.forename: Sergio
authors.initials: S
authors.lastname: Podgaec
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D017209:Apoptosis; D016022:Case-Control Studies; D049109:Cell Proliferation; D012809:Colon, Sigmoid / Q000201:enzymology / Q000473:pathology*; D003430:Cross-Sectional Studies; D004250:DNA Topoisomerases, Type II / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis*; D004715:Endometriosis / Q000201:enzymology / Q000473:pathology*; D004717:Endometrium / Q000201:enzymology / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008875:Middle Aged; D000075223:Poly-ADP-Ribose Binding Proteins; D011446:Prospective Studies; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D012007:Rectum / Q000201:enzymology / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53; C495901:TP53 protein, human; C508053:ERBB2 protein, human; C000618811:TOP2A protein, human
keywords: apoptosis; bowel endometriosis; cell proliferation; deep infiltrating endometriosis; topoisomerase 2-α