title: A time-course study of behavioral and electrophysiological characteristics in a mouse model of different stages of Parkinson's disease using 6-hydroxydopamine.
abstract: Parkinson's disease (PD) is characterized by abnormal motor symptoms and increased neuronal activity in the subthalamic nucleus (STN) as the disease progresses. We investigated the behavioral and electrophysiological characteristics in a mouse model mimicking the progressive stages of human PD (early, moderate, and advanced) by injecting 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle (MFB) at three different concentrations (2, 4, and 6 μg/2 μl). Significant changes in motor symptoms were demonstrated between groups in association with relative TH-positive cell loss in the substantia nigra pars compacta (SNc). Moreover, electrophysiologically assessed changes in the mean neuronal firing rate in the STN neurons were comparable to those in the early to advanced stages of human PD. Thus, the mouse model presented herein replicates the unique characteristics of each progressive stage of PD, in both motor and neurophysiological aspects, and therefore can be useful for further investigations of PD pathology.
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Sunghee Estelle
authors.initials: SE
authors.lastname: Park
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Electrical and Electronic Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Kang-Il
authors.initials: KI
authors.lastname: Song
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Jun-Kyo Francis
authors.initials: JK
authors.lastname: Suh
authors.affiliation: Department of Electrical and Electronic Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Dosik
authors.initials: D
authors.lastname: Hwang
authors.affiliation: Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Korea University of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. Electronic address: iyoun@kist.re.kr.
authors.forename: Inchan
authors.initials: I
authors.lastname: Youn
mesh_terms: D000200:Action Potentials; D000818:Animals; D001835:Body Weight; D018450:Disease Progression; D007150:Immunohistochemistry; D008297:Male; D008474:Medial Forebrain Bundle / Q000473:pathology / Q000503:physiopathology; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D009043:Motor Activity / Q000502:physiology; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000473:pathology / Q000503:physiopathology*; D065842:Pars Compacta / Q000473:pathology / Q000503:physiopathology*; D020531:Subthalamic Nucleus / Q000473:pathology / Q000503:physiopathology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase
keywords: 6-Hydroxydopamine; Parkinson's disease; Staged mouse model; Subthalamic nucleus; TH immunohistochemistry