title: A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.
abstract: We here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining assays directly. Using these preparations, in combination with conventional fluorescence staining techniques, we could detect the expression and subcellular localization of a specific protein and identify in situ proliferative or apoptotic cells in the germinal layer of metacestode vesicles. Based on this approach, future molecular and cellular analysis of Echinococcus metacestode vesicles in the in vitro system will be greatly facilitated.
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Medical College, Xiamen University, Xiamen, Fujian, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Medical College, Xiamen University, Xiamen, Fujian, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D048210:Echinococcus multilocularis / Q000166:cytology / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D015801:Helminth Proteins / Q000378:metabolism*; D006801:Humans; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D010766:Phosphorylation; D015203:Reproducibility of Results; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D014404:Tubulin; D048049:Extracellular Signal-Regulated MAP Kinases