title: Immunohistochemical Expression of CD105 and TGF-β1 in Oral Squamous Cell Carcinoma and Adjacent Apparently Normal Oral Mucosa and its Correlation With Clinicopathologic Features.
abstract: Angiogenesis in oral squamous cell carcinomas (OSCC) is essential for its growth, invasion, and metastasis. This entails a shift in the balance between proangiogenic and antiangiogenic factors. CD105 and TGF-β1 are 2 such proangiogenic factors wherein CD105 exerts its angiogenic effect by binding to and modulating the TGF-β1 pathway. A total of 50 resected specimens of OSCC were considered. One tissue specimen was taken from tumor proper and another specimen from adjacent apparently normal mucosa (AANM). Both tissues were immunohistochemically stained using CD105 and TGF-β1 antibodies. The expression of each antibody was individually assessed and then compared. Pearson χ test was used for statistical comparison of expression. CD105 was significantly expressed in OSCC as compared with AANM and also correlated with increasing TNM stage. The mean microvessel density was higher in OSCC. TGF-β1 was significantly expressed in epithelium of OSCC as compared with AANM. On comparing expression of TGF-β1 and CD105, 79.54% of endothelial cells expressed positivity for both molecules. Both CD105 and TGF-β1 were increased in OSCC, although based on our results CD105 alone can be used as a prognostic marker. On the basis of immunohistochemical expression of CD105 and TGF-β1 in endothelial cells, our results demonstrate that CD105 acts as one of the receptors of TGF-β1 on endothelial cells and induces the angiogenic pathway in OSCC.
authors.affiliation: Departments of *Oral Pathology and Microbiology †Molecular Biology, Maratha Mandal's NGH Institute of Dental Sciences, Belgaum, Karnataka, India.
authors.forename: Sindhu
authors.initials: S
authors.lastname: Nair
authors.forename: Ramakant
authors.initials: R
authors.lastname: Nayak
authors.forename: Kishore
authors.initials: K
authors.lastname: Bhat
authors.forename: Vijayalakshmi S
authors.initials: VS
authors.lastname: Kotrashetti
authors.forename: Deepa
authors.initials: D
authors.lastname: Babji
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000071063:Endoglin; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D009062:Mouth Neoplasms / Q000098:blood supply / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000235:genetics*; D012189:Retrospective Studies; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1