title: Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement.
abstract: Costunolide, the main bioactive compound of the medicinal plant, Radix Aucklandiae, is a sesquiterpene lactone (SL) and has a broad range of biological activities. It is also a precursor of many biologically-active SLs and is a branching point in the biosynthesis of SLs. Here we have reconstituted the costunolide biosynthetic pathway in Escherichia coli by co-expression of three genes (GAS, GAO, LsCOS) involved in costunolide biosynthesis and eight genes involved in converting acetyl-CoA into farnesyl diphosphate from mevalonate pathway. Costunolide production was then detected. By screening and optimization of cultured medium and inducing temperature, costunolide yield was up to 100 mg l(-1) in E. coli.
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 Xiqidao, Airport Economic Park, Tianjin, 300308, People's Republic of China, yin_h@tib.cas.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Zhuang
authors.forename: E-E
authors.initials: EE
authors.lastname: Li
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Bi
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism*; D007783:Lactones
chemical_list: D012717:Sesquiterpenes; C002602:costunolide; D007783:Lactones