title: A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK.
abstract: OBJECTIVE
  Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection.
  
  
  RESULTS
  We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 µl of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines.
  
  
  CONCLUSIONS
  The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.
authors.affiliation: Clinical Pharmacy of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.
authors.forename: Zhan-Zhang
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: De-Wei
authors.initials: DW
authors.lastname: Shang
authors.forename: Xiao-Jia
authors.initials: XJ
authors.lastname: Ni
authors.forename: Jin-Qing
authors.initials: JQ
authors.lastname: Hu
authors.forename: Chang
authors.initials: C
authors.lastname: Qiu
authors.forename: Yu-Guan
authors.initials: YG
authors.lastname: Wen
mesh_terms: D000146:Acids, Carbocyclic; D000328:Adult; D053000:Analytic Sample Preparation Methods / Q000191:economics*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D003517:Cyclopentanes / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification* / Q000493:pharmacokinetics; D006146:Guanidines / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000146:Acids, Carbocyclic; D003517:Cyclopentanes; D006146:Guanidines; C414210:peramivir