title: Effects of saliva on early post-tooth extraction tissue repair in rats.
abstract: The aim of the present study was to perform a biochemical, histological, and histomorphometrical evaluation of the mechanisms involved in tissue repair in rats subjected to submandibulectomy-induced hyposialia, 24, 48, and 72 hours of post-tooth extraction. We studied the correlation between the lack of submandibular saliva and the modulation of inflammatory mediators involved in tissue repair, such as prostaglandin E2 , nitric oxide (NO), and tumor necrosis factor alpha (TNF-α). Rats with hyposialia showed a delay in socket healing, slow replacement of the clot with granulation tissue, and fewer cells and collagen fibers, concomitant with a longer inflammatory process, as compared to controls. The lack of saliva induced by submandibulectomy modified the levels of prostaglandin E2 , NO, and TNF-α, and tissue response in the early stages of wound healing compared to controls, and could thus determine alterations in later osteogenic response. Our results allow concluding that hyposialia modulates the parameters of inflammation studied here, and that it is essential for optimal healing. Therefore, these findings provide evidence for the importance of submandibular saliva to final bone socket healing.
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Argentina.|National Council for Scientific and Technological Research, Argentina.
authors.forename: Claudia E
authors.initials: CE
authors.lastname: Mohn
authors.affiliation: Department of Oral Pathology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Tammy
authors.initials: T
authors.lastname: Steimetz
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Argentina.
authors.forename: Pablo N
authors.initials: PN
authors.lastname: Surkin
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Argentina.|National Council for Scientific and Technological Research, Argentina.
authors.forename: Javier
authors.initials: J
authors.lastname: Fernandez-Solari
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Argentina.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Elverdin
authors.affiliation: National Council for Scientific and Technological Research, Argentina.|Department of Oral Pathology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: María B
authors.initials: MB
authors.lastname: Guglielmotti
mesh_terms: D000818:Animals; D006097:Granulation Tissue / Q000473:pathology*; D007249:Inflammation / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014081:Tooth Extraction; D020390:Tooth Socket / Q000276:immunology / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing; D014987:Xerostomia / Q000150:complications / Q000473:pathology*
chemical_list: D014409:Tumor Necrosis Factor-alpha