title: Engineered single-chain variable fragment antibody for immunodiagnosis of groundnut bud necrosis virus infection.
abstract: Few studies have been done on engineered antibodies for diagnosis of tospovirus infections. The present study was undertaken to develop a single-chain variable fragment (scFv) for specific diagnosis of infection by groundnut bud necrosis virus (GBNV), the most prevalent serogroup IV tospovirus in India. Heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scFv construct that expressed a ~29-kDa protein in E. coli. The scFv specifically detected GBNV in field samples of cowpea, groundnut, mung bean, and tomato, and it did not recognize watermelon bud necrosis virus, a close relative of GBNV belonging to tospovirus serogroup IV. This study for the first time demonstrated the application of a functional scFv against a serogroup-IV tospovirus.
authors.affiliation: Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India.
authors.forename: Yogita
authors.initials: Y
authors.lastname: Maheshwari
authors.forename: S
authors.initials: S
authors.lastname: Vijayanandraj
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
authors.forename: Bikash
authors.initials: B
authors.lastname: Mandal
mesh_terms: D000914:Antibodies, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007887:Fabaceae / Q000821:virology; D015870:Gene Expression; D007159:Immunologic Tests / Q000379:methods; D007194:India; D018551:Solanum lycopersicum / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D057127:Single-Chain Antibodies / Q000235:genetics; D017865:Tospovirus / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies