title: Effects of age and unaccustomed resistance exercise on mitochondrial transcript and protein abundance in skeletal muscle of men.
abstract: Mitochondrial dysfunction may contribute to age-associated muscle atrophy. Previous data has shown that resistance exercise (RE) increases mitochondrial gene expression and enzyme activity in older adults; however, the acute response to RE has not been well characterized. To characterize the acute mitochondrial response to unaccustomed RE, healthy young (21 ± 3 yr) and older (70 ± 4 yr) men performed a unilateral RE bout for the knee extensors. Muscle biopsies were taken at rest and 3, 24, and 48 h following leg press and knee extension exercise. The expression of the mitochondrial transcriptional regulator proliferator-activated receptor γ coactivator 1-α (PGC-1α) mRNA was increased at 3 h postexercise; however, all other mitochondrial variables decreased over the postexercise period, irrespective of age. ND1, ND4, and citrate synthase (CS) mRNA were all lower at 48 h postexercise, along with specific protein subunits of complex II, III, IV, and ATP synthase. Mitochondrial DNA (mtDNA) copy number decreased by 48 h postexercise, and mtDNA deletions were higher in the older adults and remained unaffected by acute exercise. Elevated mitophagy could not explain the reduction in mitochondrial proteins and DNA, because there was no increase in ubiquitinated voltage-dependent anion channel (VDAC) or its association with PTEN-induced putative kinase 1 (Pink1) or Parkin, and elevated p62 content indicated an impairment or reduction in autophagocytic flux. In conclusion, age did not influence the response of specific mitochondrial transcripts, proteins, and DNA to a bout of RE.
authors.affiliation: Departments of Medical Sciences.
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Ogborn
authors.affiliation: Kinesiology.
authors.forename: Bryon R
authors.initials: BR
authors.lastname: McKay
authors.affiliation: Kinesiology.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Crane
authors.affiliation: Cardiovascular Institute, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachussets.
authors.forename: Adeel
authors.initials: A
authors.lastname: Safdar
authors.affiliation: Pediatrics and.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Akhtar
authors.affiliation: Kinesiology.
authors.forename: Gianni
authors.initials: G
authors.lastname: Parise
authors.affiliation: Pediatrics and Medicine, McMaster University, Hamilton, ON, Canada; and tarnopol@mcmaster.ca.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Tarnopolsky
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D001343:Autophagy; D001706:Biopsy; D004272:DNA, Mitochondrial / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D055070:Resistance Training; D013997:Time Factors; D014158:Transcription, Genetic; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D012333:RNA, Messenger
keywords: autophagy; mitochondria; resistance exercise; skeletal muscle