title: Simultaneous functions of the installed DAS/DAK formaldehyde-assimilation pathway and the original formaldehyde metabolic pathways enhance the ability of transgenic geranium to purify gaseous formaldehyde polluted environment.
abstract: The overexpression of dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAK) from methylotrophic yeasts in chloroplasts created a photosynthetic formaldehyde (HCHO)-assimilation pathway (DAS/DAK pathway) in transgenic tobacco. Geranium has abilities to absorb and metabolize HCHO. Results of this study showed that the installed DAS/DAK pathway functioning in chloroplasts greatly enhanced the role of the Calvin cycle in transgenic geranium under high concentrations of gaseous HCHO stress. Consequently, the yield of sugars from HCHO-assimilation increased approximately 6-fold in transgenic geranium leaves, and concomitantly, the role of three original HCHO metabolic pathways reduced, leading to a significant decrease in formic acid, citrate and glycine production from HCHO metabolism. Although the role of three metabolic pathways reduced in transgenic plants under high concentrations of gaseous HCHO stress, the installed DAS/DAK pathway could still function together with the original HCHO metabolic pathways. Consequently, the gaseous HCHO-resistance of transgenic plants was significantly improved, and the generation of H2O2 in the transgenic geranium leaves was significantly less than that in the wild type (WT) leaves. Under environmental-polluted gaseous HCHO stress for a long duration, the stomata conductance of transgenic plants remained approximately 2-fold higher than that of the WT, thereby increasing its ability to purify gaseous HCHO polluted environment.
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China.
authors.forename: Shengen
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China.
authors.forename: Sunqin
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Xuan
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China.
authors.forename: Kunzhi
authors.initials: K
authors.lastname: Li
authors.affiliation: Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming 650500, PR China. Electronic address: chenlimeikm@126.com.
authors.forename: Limei
authors.initials: L
authors.lastname: Chen
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D019880:Aldehyde-Ketone Transferases / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002736:Chloroplasts / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism*; D005740:Gases / Q000378:metabolism; D015870:Gene Expression; D005800:Genes, Fungal; D031315:Geranium / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics; D015003:Yeasts / Q000235:genetics
chemical_list: D004785:Environmental Pollutants; D005740:Gases; D005557:Formaldehyde; D006861:Hydrogen Peroxide; D019880:Aldehyde-Ketone Transferases; C031222:formaldehyde transketolase; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase
keywords: DAS/DAK HCHO-assimilation pathway; Environmental-polluted gaseous HCHO; Gaseous HCHO; Original HCHO metabolic pathway; Transgenic geranium