title: Orosomucoid 1 drives opportunistic infections through the polarization of monocytes to the M2b phenotype.
abstract: Orosomucoid (ORM, composed of two isoforms, ORM1 and ORM2) has been described as an inducer of M2 macrophages, which are cells that decrease host antibacterial innate immunities. However, it is unknown which phenotypes of M2 macrophages are induced by ORM. In this study, healthy donor monocytes stimulated with ORM (ORM-monocytes) were characterized phenotypically and biologically. CCL1 (a biomarker of M2b macrophages) and IL-10 were detected in monocyte cultures supplemented with ORM1; however, CCL17 (a biomarker of M2a macrophages) and CXCL13 (a biomarker of M2c macrophages) were not produced in these cultures. All of these soluble factors were not detected in the culture fluids of monocytes stimulated with ORM2. Monocytes stimulated with ORM1 were characterized as CD64(-)CD209(-)CD163(+)CCL1(+) cells. MRSA and Enterococcus faecalis infections were accelerated in chimeras (NOD/scid IL-2Rγ(null) mice reconstituted with white blood cells) after inoculation with monocytes stimulated with ORM1 or treatment with ORM1; however, the infections were greatly mitigated in both chimeras inoculated with ORM1-stimulated monocytes and treated with ORM1, after an additional treatment with an inhibitor of M2b macrophages (CCL1 antisense ODN). These results indicate that ORM1 stimulates quiescent monocytes to polarize to M2b monocytes. The regulation of M2b macrophages may be beneficial in controlling opportunistic infections in patients with a large amount of plasma ORM1.
authors.affiliation: Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555, United States.
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555, United States.
authors.forename: Ichiaki
authors.initials: I
authors.lastname: Ito
authors.affiliation: Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555, United States; Shriners Hospital for Children, Galveston, TX 77550, United States.
authors.forename: Makiko
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Shriners Hospital for Children, Galveston, TX 77550, United States.
authors.forename: David N
authors.initials: DN
authors.lastname: Herndon
authors.affiliation: Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555, United States; Shriners Hospital for Children, Galveston, TX 77550, United States. Electronic address: fsuzuki@utmb.edu.
authors.forename: Fujio
authors.initials: F
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D016764:Cell Polarity / Q000187:drug effects*; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis; D013293:Enterococcus faecalis / Q000187:drug effects; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000473:pathology*; D009894:Opportunistic Infections / Q000382:microbiology / Q000473:pathology*; D009961:Orosomucoid / Q000494:pharmacology*; D010641:Phenotype
chemical_list: D018925:Chemokines; D009961:Orosomucoid
keywords: CCL1; Monocytes; Orosomucoid