title: Core fucosylation of IgG B cell receptor is required for antigen recognition and antibody production.
abstract: Ag recognition and Ab production in B cells are major components of the humoral immune response. In the current study, we found that the core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) was required for the Ag recognition of BCR and the subsequent signal transduction. Moreover, compared with the 3-83 B cells, the coalescing of lipid rafts and Ag-BCR endocytosis were substantially reduced in Fut8-knockdown (3-83-KD) cells with p31 stimulation and then completely restored by reintroduction of the Fut8 gene to the 3-83-KD cells. Indeed, Fut8-null (Fut8(-/-)) mice evoked a low immune response following OVA immunization. Also, the frequency of IgG-producing cells was significantly reduced in the Fut8(-/-) spleen following OVA immunization. Our results clearly suggest an unexpected mode of BCR function, in which the core fucosylation of IgG-BCR mediates Ag recognition and, concomitantly, cell signal transduction via BCR and Ab production.
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China; liwenzhe46@hotmail.com.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Biao
authors.initials: B
authors.lastname: Ma
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Xinyan
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710127, China;
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Dalian University, Liaoning 116622, China;
authors.forename: Hongyu
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Weijie
authors.initials: W
authors.lastname: Dong
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Linhua
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Keli
authors.initials: K
authors.lastname: Ma
authors.affiliation: Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Miyagi 981-8558, Japan;
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: College of Life Science and Technology, Dalian University, Liaoning 116622, China;
authors.forename: Qingping
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Liaoning 116044, China;
authors.forename: Tonghui
authors.initials: T
authors.lastname: Ma
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710127, China;
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Miyagi 981-8558, Japan;
authors.forename: Jianguo
authors.initials: J
authors.lastname: Gu
authors.affiliation: School of Life Science and Medicine, Dalian University of Technology, Panjin 124221, China; and.
authors.forename: Jianing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Systems Glycobiology Research Group, Advanced Science Institute, RIKEN, Saitama 351-0198, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002478:Cells, Cultured; D004705:Endocytosis / Q000276:immunology; D005434:Flow Cytometry; D005643:Fucose / Q000276:immunology / Q000378:metabolism; D005647:Fucosyltransferases / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D006031:Glycosylation; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D021962:Membrane Microdomains / Q000276:immunology / Q000378:metabolism; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000378:metabolism
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D005643:Fucose; D010047:Ovalbumin; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase