title: Five new secoiridoid glycosides and one unusual lactonic enol ketone with anti-HBV activity from Swertia cincta.
abstract: Five new secoiridoid glycosides, swericinctosides A and B (1-2), 9-epi swertiamarin (3), 2'-O-m-hydroxybenzoyl swertiamarin (4), 4″-O-acetyl swertianoside E (5), and one unusual lactonic enol ketone, 3-(hydroxymethyl ene) dihydro-2H-pyran-2, 4(3H)-dione (6), together with three known compounds, swertiaside (7), swertianoside C (8) and decentapicrin B (9) were isolated from Swertia cincta. The structures of the new compounds were determined by extensive spectroscopic analyses including 1D and 2D NMR, HRESIMS, UV, IR and [α]D spectra. Anti-HBV assay on HepG 2.2.15 cell line in vitro demonstrated that compounds 1-9 possessed inhibitory activity on HBV DNA replication with IC50 values from 0.05 to 1.83mM, and compounds 1, 3, 5, 7 and 8 could inhibit the secretion of HBsAg with IC50 values from 0.24 to 1.06mM.
authors.affiliation: School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jie
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Yun-Bao
authors.initials: YB
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, PR China. Electronic address: zhrpkm@163.com.
authors.forename: Rong-Ping
authors.initials: RP
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China. Electronic address: chenjj@mail.kib.ac.cn.
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D057889:Iridoid Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D007659:Ketones / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D029967:Swertia / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D057889:Iridoid Glycosides; D007659:Ketones
keywords: Anti-HBV activity; Lactonic enol ketone; Secoiridoid glycosides; Swertia cincta