title: Oral bacteria adherence to suture threads: an in vitro study.
abstract: OBJECTIVE
  This study investigated the adherence of oral microorganisms to different types of suture threads.
  
  
  METHODS
  Pieces of thread were distributed on 24-well plates, according to the following groups: (G1) nylon, (G2) silk, (G3) polyglactin 910, (G4) polyglactin 910 with triclosan. Blank control (G5) consisted of one thread from each group. Adherence to thread tests was performed to observe adhesion of total microorganisms from saliva or two isolates of Prevotella intermedia (ATCC49046) and Fusobacterium nucleatum (ATCC51190). Brain-heart infusion (BHI) medium with or without bacterial inoculum (1.8 × 10(7) CFU/mL) was added to each well of microplates. The microplates were incubated in an anaerobic chamber at 37 °C, for 5 days for biofilm formation.
  
  
  RESULTS
  There was no difference between the groups as regard to adhesion of F. nucleatum (p > 0.05). For P. intermedia, the threads in G1 and G4 showed a lower level of adhesion (p < 0.05), with no difference between them. Against total microorganisms, G1 presented a lower level of adherence (p < 0.05), followed by G4; and no difference was observed between G2 and G3.
  
  
  CONCLUSIONS
  Total microorganisms and P. intermedia have different affinities to the tested suture threads, whereas F. nucleatum presented a similar adherence level. Among the threads, nylon (G1), followed by polyglactin 910 with triclosan (G4) presented the lowest microbial adherence level.
authors.affiliation: Department of Post-graduation, Professional Master's Degree in Dentistry, School of Dentistry, "Universidade Veiga de Almeida", Rio de Janeiro, Brazil.
authors.forename: Oswaldo
authors.initials: O
authors.lastname: de Castro Costa Neto
authors.forename: Leandro Araujo
authors.initials: LA
authors.lastname: Lobo
authors.forename: Natalia Lopes Pontes
authors.initials: NL
authors.lastname: Iorio
authors.forename: Maria
authors.initials: M
authors.lastname: de Fátima Carvalho Vasconcelos
authors.forename: Lucianne Cople
authors.initials: LC
authors.lastname: Maia
authors.forename: Patrícia Nivoloni
authors.initials: PN
authors.lastname: Tannure
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Antonio
mesh_terms: D001422:Bacterial Adhesion; D058491:Bacterial Load; D016967:Fusobacterium nucleatum; D006801:Humans; D066298:In Vitro Techniques; D018713:Prevotella intermedia; D012463:Saliva / Q000382:microbiology; D013537:Sutures / Q000382:microbiology*