title: Development and characterization of microsatellite markers for Piptadenia gonoacantha (Fabaceae).
abstract: •
  
  
  OBJECTIVE
  Microsatellite primers were designed for Piptadenia gonoacantha (Fabaceae) and characterized to estimate genetic diversity parameters. The species is a native tree from the Atlantic Forest biome commonly used in forest restoration; it has medicinal potential and the wood is economically useful. •
  
  
  RESULTS
  Twenty-eight microsatellite loci were identified from an enriched genomic library. Fifteen loci resulted in successful amplifications and were characterized in a natural population of 94 individuals. Twelve loci were polymorphic, with allele numbers ranging from three to 15 per locus, and expected and observed heterozygosities ranging from 0.2142 to 0.8325 and 0.190 to 0.769, respectively. •
  
  
  CONCLUSIONS
  The developed markers will be used in further studies of population genetics of P. gonoacantha, aimed at conservation and management of the species in natural populations and in forest restoration projects.
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Grando
authors.affiliation: Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Miklos M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Stephanie K
authors.initials: SK
authors.lastname: Bajay
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Kaiser D
authors.initials: KD
authors.lastname: Schwarcz
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Jaqueline B
authors.initials: JB
authors.lastname: Campos
authors.affiliation: Departamento de Ciências Florestais, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Pedro H S
authors.initials: PH
authors.lastname: Brancalion
authors.affiliation: Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: José B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Departamento de Ciências Florestais, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Ricardo R
authors.initials: RR
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-graduação em Biologia Vegetal, Universidade Estadual de Campinas, CP 6109, Rua Bertrand Russel, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Pólo Centro Sul, CP 28, Rodovia SP 127, Km 30, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zucchi
keywords: Atlantic Forest; Fabaceae; Piptadenia gonoacantha; conservation genetics; forest restoration; microsatellites