title: Immunohistochemical Expressions of MUC2, MUC5AC, and MUC6 in Normal, Helicobacter pylori Infected and Metaplastic Gastric Mucosa of Children and Adolescents.
abstract: OBJECTIVE
  The aim of this study was to investigate expression of gastric mucins in children and adolescents and to assess their relations with age and Helicobacter pylori (H. pylori) infection.
  
  
  METHODS
  Gastric biopsies were collected from 259 pediatric and adulthood patients with gastrointestinal symptoms among all of patients undergone gastroduodenoscopy from 1990 to 2004 at Gyeongsang National University hospital and assorted based on H. pylori infection, age, and intestinal metaplasia as follows; H. pylori infection before 5 years of age or not, H. pylori infection between 5 and 9 years of age or not, H. pylori infection between 10 and 14 years of age or not, H. pylori infection between 20 and 29 years of age or not and intestinal metaplasia between 21 and 35 years of age. Total 810 tissue slides from the subjects were examined regarding expressions of Mucin2 (MUC2), Mucin5AC (MUC5AC), and Mucin6 (MUC6) in nine groups using immunohistochemical stains. A semiquantitative approach was used to score the staining extent of tissue slide.
  
  
  RESULTS
  Increased expressions of MUC2, MUC5AC, and MUC6 were noted in intestinal metaplasia compared with subjects infected with H. pylori between 20 and 29 years. Gastric expressions of MUC5AC were decreased in older than 5 years with H. pylori compared with in older than 5 years without H. pylori (p < .001). Expressions of MUC2 and MUC6 did not change significantly by H. pylori status. Some nuclear expressions of MUC2 and MUC6 were noted in children without intestinal metaplasia.
  
  
  CONCLUSIONS
  MUC5AC might be affected by chronic H. pylori infection. In addition to biomarkers for intestinal metaplasia or prognostic factors for gastric cancer in adults, MUC2 and MUC6 in children might have an another role, based on ectopic gastric nuclear expressions of MUC2 and MUC6 in children without intestinal metaplasia.
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Ji Sook
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Yeom
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lim
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Chan-Hoo
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Hyang-Ok
authors.initials: HO
authors.lastname: Woo
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Jun
authors.affiliation: Department of Pediatrics, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Ji-Hoe
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Pathology, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Gyung-Hyuck
authors.initials: GH
authors.lastname: Ko
authors.affiliation: Department of Microbiology, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.affiliation: Department of Microbiology, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.affiliation: Department of Microbiology, Gyeonsang National University School of Medicine, Institute of Health Science, Gyeongsang National University Hospital, Jinju, Gyeongsangnam-do, Korea.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging; D002648:Child; D002675:Child, Preschool; D005260:Female; D005752:Gastric Mucins / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D016481:Helicobacter Infections / Q000235:genetics / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D007422:Intestines / Q000473:pathology; D008297:Male; D008679:Metaplasia / Q000473:pathology; D055271:Mucin 5AC / Q000378:metabolism*; D055262:Mucin-2 / Q000378:metabolism*; D055272:Mucin-6 / Q000378:metabolism*; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000473:pathology; D055815:Young Adult
chemical_list: D005752:Gastric Mucins; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6
keywords: Helicobacter pylori; Immunohistochemistry; gastric mucin