title: [The process of heme synthesis in bone marrow mesenchymal stem cells cultured under fibroblast growth factor bFGF and hypoxic conditions].
abstract: It was demonstrated that fibroblast growth factor bFGF influences the process of heme synthesis, the proliferation activity and viability of bone marrow mesenchymal stem cells in culture under hypoxic conditions. The addition of fibroblast growth factor bFGF (7 ng/ml) to the medium under above conditions led to the accumulation of aminolevulinic acid--an early porphyrin and heme precursor, an increase in CD 71 expression--a transferrin receptor, and also a decrease in porphyrin pigments and heme contents--a late precursor and end products of heme synthesis, respectively. It was found that cultivation of the cells under hypoxic conditions and bFGF is an optimum to maintain high viability and proliferation capacity of the mesenchymal stem cells.
authors.forename: A G
authors.initials: AG
authors.lastname: Poleshko
authors.forename: E S
authors.initials: ES
authors.lastname: Lobanok
authors.forename: L M
authors.initials: LM
authors.lastname: Mezhevikina
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
authors.forename: I D
authors.initials: ID
authors.lastname: Volotkovskiĭ
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006418:Heme / Q000096:biosynthesis*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011990:Receptors, Transferrin / Q000096:biosynthesis
chemical_list: D015703:Antigens, CD; C068126:CD71 antigen; D011990:Receptors, Transferrin; D016222:Fibroblast Growth Factor 2; D006418:Heme; D000622:Aminolevulinic Acid