title: Kinetics of glucosylated and non-glucosylated aryltetralin lignans in Linum hairy root cultures.
abstract: Due to their pronounced cytotoxic activity, a number of aryltetralin lignans (ATLs), such as podophyllotoxin (PTOX), are used as antitumor compounds. The production of such molecules from entire plants or plant cell-tissue-organ cultures is thus of interest to the pharmaceutical industry. Hairy root cultures constitute a good tool not only for phytochemical production but also for investigating plant secondary metabolism. This work reports on the growth and ATL biosynthesis in two hairy root cultures of Linum album Kotschy ex Boiss. and Linum flavum. The kinetics of accumulation of the intermediates of MPTOX biosynthesis and of their glucosylated forms are described over a 21-day period of growth. An accumulation of non-glucosylated forms of the ATLs during the exponential phase of the cultures is followed by an accumulation of the glucosylated forms during the stationary phase. Our results show a strong coordination of the biosynthetic paths derived from deoxypodophyllotoxin via deoxypodophyllotoxin 6-hydroxylase and deoxypodophyllotoxin 7-hydroxylase, and a coordinated glucosylation of podophyllotoxin, methoxypodophyllotoxin, and 5'-demethoxymethoxypodophyllotoxin. Furthermore, our results suggest an important role of β-peltatin-6-glucoside formation in the control of ATL accumulation in Linum hairy root cultures.
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Luyen Huynh
authors.initials: LH
authors.lastname: Cong
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Dauwe
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Lequart
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Vinchon
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France; Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Sullivan
authors.initials: S
authors.lastname: Renouard
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Ophélie
authors.initials: O
authors.lastname: Fliniaux
authors.affiliation: Fédération de Recherche Physique et Chimie du Vivant, Université d'Orléans-CNRS, FR 2708, rue de Chartres, 45067 Orléans Cedex 2, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Colas
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Cyrielle
authors.initials: C
authors.lastname: Corbin
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France; Ecole SITI (Département CASER), Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75141 Paris Cedex 03, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Doussot
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hano
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC) - Université d'Orléans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lamblin
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Molinié
authors.affiliation: Plate-forme analytique, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Pilard
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Jullian
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Boitel
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gontier
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: François
authors.initials: F
authors.lastname: Mesnard
authors.affiliation: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France. Electronic address: jean-claude.laberche@u-picardie.fr.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Laberche
mesh_terms: D004365:Drugs, Chinese Herbal; D019597:Flax / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D006031:Glycosylation; D007700:Kinetics; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D011034:Podophyllotoxin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity
chemical_list: D004365:Drugs, Chinese Herbal; D017705:Lignans; C014451:deoxypodophyllotoxin; D011034:Podophyllotoxin
keywords: Aryltetralin lignans; Correlation analysis; Hairy root cultures; Linum album; Linum flavum; Podophyllotoxin