title: [Interactions between proteins and cation exchange adsorbents analyzed by NMR and hydrogen/deuterium exchange technique].
abstract: In silico acquirement of the accurate residue details of protein on chromatographic media is a bottleneck in protein chromatography separation and purification. Here we developed a novel approach by coupling with H/D exchange and nuclear magnetic resonance to observe hen egg white lysozyme (HEWL) unfolding behavior adsorbed on cation exchange media (SP Sepharose FF). Analysis of 1D 1H-NMR shows that protein unfolding accelerated H/D exchange rate, leading to more loss of signal of amide hydrogen owing to exposure of residues and the more unfolding of protein. Analysis of two-dimensional hydrogen-hydrogen total correlation spectroscopy shows that lysozyme lost more signals and experienced great unfolding during its adsorption on media surface. However, for several distinct fragments, the protection degrees varied, the adsorbed lysozyme lost more signal intensity and was less protected at disorder structures (coil, bend, and turn), but was comparatively more protected against exchange at secondary structure domains (α-helix, β-sheet). Finally, the binding site was determined by electrostatic calculations using computer simulation methods in conjunction with hydrogen deuterium labeled protein and NMR. This study would help deeply understand the microscopic mechanism of protein chromatography and guide the purposely design of chromatographic process and media. Moreover, it also provide an effective tool to study the protein and biomaterials interaction in other applications.
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.forename: Dongxia
authors.initials: D
authors.lastname: Hao
authors.forename: Shuting
authors.initials: S
authors.lastname: Qi
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
mesh_terms: D000327:Adsorption; D000577:Amides; D002412:Cations; D003198:Computer Simulation; D003903:Deuterium; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000737:chemistry; D017433:Protein Structure, Secondary; D058767:Protein Unfolding; D011506:Proteins / Q000737:chemistry*
chemical_list: D000577:Amides; D002412:Cations; D011506:Proteins; D006859:Hydrogen; D003903:Deuterium; C421864:hen egg lysozyme; D009113:Muramidase