title: Isolation and characterization of arylacetamide deacetylase in cynomolgus macaques.
abstract: Arylacetamide deacetylase (AADAC), a microsomal serine esterase, hydrolyzes drugs, such as flutamide, phenacetin and rifampicin. Because AADAC has not been fully investigated at molecular levels in cynomolgus macaques, the non-human primate species widely used in drug metabolism studies, cynomolgus AADAC cDNA was isolated and characterized. The deduced amino acid sequence, highly homologous (92%) to human AADAC, was more closely clustered with human AADAC than the dog, rat or mouse ortholog in a phylogenetic tree. AADAC was flanked by AADACL2 and SUCNR1 in the cynomolgus and human genomes. Moreover, relatively abundant expression of AADAC mRNA was found in liver and jejunum, the drug-metabolizing organs, in cynomolgus macaques, similar to humans. The results suggest molecular similarities of AADAC between cynomolgus macaques and humans.
authors.affiliation: Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Wakayama 642-0017, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Uno
authors.forename: Masakiyo
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Teruko
authors.initials: T
authors.lastname: Imai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007583:Jejunum / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008252:Macaca fascicularis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; C569302:AADAC protein, human; D002265:Carboxylic Ester Hydrolases