title: Spatially resolved quantification of gadolinium(III)-based magnetic resonance agents in tissue by MALDI imaging mass spectrometry after in vivo MRI.
abstract: Gadolinium(III)-based contrast agents improve the sensitivity and specificity of magnetic resonance imaging (MRI), especially when targeted contrast agents are applied. Because of nonlinear correlation between the contrast agent concentration in tissue and the MRI signal obtained in vivo, quantification of certain biological or pathophysiological processes by MRI remains a challenge. Up to now, no technology has been able to provide a spatially resolved quantification of MRI agents directly within the tissue, which would allow a more precise verification of in vivo imaging results. MALDI imaging mass spectrometry for spatially resolved in situ quantification of gadolinium(III) agents, in correlation to in vivo MRI, were evaluated. Enhanced kinetics of Gadofluorine M were determined dynamically over time in a mouse model of myocardial infarction. MALDI imaging was able to corroborate the in vivo imaging MRI signals and enabled in situ quantification of the gadolinium probe with high spatial resolution.
authors.affiliation: Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstaedter Landstrasse 1, 85764 Neuherberg (Germany).
authors.forename: Michaela
authors.initials: M
authors.lastname: Aichler
authors.forename: Katharina
authors.initials: K
authors.lastname: Huber
authors.forename: Franz
authors.initials: F
authors.lastname: Schilling
authors.forename: Fabian
authors.initials: F
authors.lastname: Lohöfer
authors.forename: Katja
authors.initials: K
authors.lastname: Kosanke
authors.forename: Reinhard
authors.initials: R
authors.lastname: Meier
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Rummeny
authors.forename: Axel
authors.initials: A
authors.lastname: Walch
authors.forename: Moritz
authors.initials: M
authors.lastname: Wildgruber
mesh_terms: D003287:Contrast Media; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D003287:Contrast Media; D005682:Gadolinium
keywords: biochemistry; gadolinium; imaging agents; kinetics; mass spectrometry