title: Multiple applications of ion chromatography oligosaccharide fingerprint profiles to solve a variety of sugar and sugar-biofuel industry problems.
abstract: Sugar crops contain a broad variety of carbohydrates used for human consumption and the production of biofuels and bioproducts. Ion chromatography with integrated pulsed amperometric detection (IC-IPAD) can be used to simultaneously detect mono-, di-, and oligosaccharides, oligosaccharide isomers, mannitol, and ethanol in complex matrices from sugar crops. By utilizing a strong NaOH/NaOAc gradient method over 45 min, oligosaccharides of at least 2-12 dp can be detected. Fingerprint IC oligosaccharide profiles are extremely selective, sensitive, and reliable and can detect deterioration product metabolites from as low as 100 colony-forming units/mL lactic acid bacteria. The IC fingerprints can also be used to (i) monitor freeze deterioration, (ii) optimize harvesting methods and cut-to-crush times, (iii) differentiate between white refined sugar from sugar cane and from sugar beets, (iv) verify the activities of carbohydrate enzymes, (v) select yeasts for ethanol fermentations, and (vi) isolate and diagnose infections and processing problems in sugar factories.
authors.affiliation: †Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Gillian
authors.initials: G
authors.lastname: Eggleston
authors.affiliation: §Fermentec Ltda., Av. Antônia Pazzinato Sturion 1155, Piracicaba, Brazil 13420 640.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Borges
mesh_terms: D056804:Biofuels / Q000032:analysis*; D002241:Carbohydrates / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D007536:Isomerism; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D009844:Oligosaccharides
keywords: HPAEC; ion chromatography; oligosaccharide fingerprint profiles; pulsed amperometric detection; sugar beet; sugar cane; sweet sorghum