title: Influence of peptides-phenolics interaction on the antioxidant profile of protein hydrolysates from Brassica napus.
abstract: The role of the peptides-phenolic compounds (PC) interaction on the antioxidant capacity profile (ACP) of protein hydrolysates from rapeseed (Brassica napus) was studied in 36 hydrolysates obtained from a PC-rich and PC-reduced protein substrate. The latent profile analysis (LPA), with data of seven in vitro methods and one assay for cellular antioxidant activity (CAA), allowed identifying five distinctive groups of hydrolysates, each one with distinctive ACP. The interaction of peptides with naturally present PC diminished in vitro antioxidant activity in comparison with their PC-reduced counterparts. However, CAA increased when peptides-PC interaction occurred. The profile with the highest average CAA (62.41 ± 1.48%), shown by hydrolysates obtained by using alcalase, shared typical values of Cu(2+)-catalysed β-carotene oxidation (62.41 ± 0.43%), β-carotene bleaching inhibition (91.75 ± 0.22%) and Cu(2+)-chelating activity (74.53 ± 0.58%). The possibilities for a sample to exhibit ACP with higher CAA increased with each unit of positively charged amino acids, according to multinomial logistic regression analysis.
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, D.F., Mexico.
authors.forename: Anaid
authors.initials: A
authors.lastname: Hernández-Jabalera
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Cortés-Giraldo
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, D.F., Mexico.
authors.forename: Gloria
authors.initials: G
authors.lastname: Dávila-Ortíz
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Vioque
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Alaiz
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Girón-Calle
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Megías
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, D.F., Mexico. Electronic address: crisjm_99@yahoo.com.
authors.forename: Cristian
authors.initials: C
authors.lastname: Jiménez-Martínez
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry*; D029688:Brassica napus / Q000737:chemistry*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D010455:Peptides; D010636:Phenols; D011492:Protein Hydrolysates
keywords: Antioxidant activity; Brassica napus; Latent profile analysis; Phenolic–peptides interaction; Protein hydrolysates