title: Gold nanoparticles immobilized on metal-organic frameworks with enhanced catalytic performance for DNA detection.
abstract: In this work, gold nanoparticles (AuNPs) assembled on the surface of iron based metal-organic frameworks (MOFs), Fe-MIL-88, are facilely prepared through electrostatic interactions using polyethyleneimine (PEI) molecules as linker. The resulting hybrid materials possess synergetic peroxidase-like activity. Because iron based metal-organic frameworks, Fe-MIL-88, exhibits highly peroxidase-like activity, and AuNPs has the distinct adsorption property to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The peroxidase-like activity of Au@Fe-MIL-88 exhibit excellent switchable in response to specific DNA, ssDNA is easily adsorbed on the surface of the Au@Fe-MIL-88 hybrids, resulting in the reduce of the peroxidase-like activity of the hybrids. While it is recovered by the addition of target DNA, and the recovery degree is proportional to the target DNA concentration over the range of 30-150 nM with a detection limit of 11.4 nM. Based on these unique properties, we develop a label-free colorimetric method for DNA hybridization detection. In control experiment, base-mismatched DNA cannot induce recovery of the peroxidase-like activity. This detection method is simple, cheap, rapid and colorimetric.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ya Li
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Wen Liang
authors.initials: WL
authors.lastname: Fu
authors.affiliation: College of Pharmaceutical Science, Southwest University, Chongqing 400715, China.
authors.forename: Chun Mei
authors.initials: CM
authors.lastname: Li
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; College of Pharmaceutical Science, Southwest University, Chongqing 400715, China.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: liyf@swu.edu.cn.
authors.forename: Yuan Fang
authors.initials: YF
authors.lastname: Li
mesh_terms: D000327:Adsorption; D002384:Catalysis; D004279:DNA, Viral / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006678:HIV / Q000235:genetics; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D009930:Organic Chemicals / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D009930:Organic Chemicals; D006046:Gold
keywords: DNA detection; Gold nanoparticles; Metal–organic frameworks; Peroxidase mimetic