title: Biosorption and biodegradation of pyrene by Brevibacillus brevis and cellular responses to pyrene treatment.
abstract: Biodegradation has been proposed as an effective approach to remove pyrene, however, the information regarding cellular responses to pyrene treatment is limited thus far. In this study, the biodegradation and biosorption of pyrene by Brevibacillus brevis, along with cellular responses caused by pollutant were investigated by means of flow cytometry assay and scanning electron microscopy. The experimental results showed that pyrene was initially adsorbed by B. brevis and subsequently transported and intracellularly degraded. During this process, pyrene removal was primarily dependent on biodegradation. Cell invagination and cell surface corrugation occurred due to pyrene exposure. Nevertheless, cell regrowth after 96h treatment was observed, and the proportion of necrotic cell was only 2.8% after pyrene exposure for 120h, confirming that B. brevis could utilize pyrene as a sole carbon source for growth. The removal and biodegradation amount of pyrene (1mg/L) at 168h were 0.75 and 0.69mg/L, respectively, and the biosorption amount by inactivated cells was 0.41mg/L at this time.
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Shuona
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, Guangdong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China. Electronic address: huayin@scut.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China. Electronic address: jsye@jnu.edu.cn.
authors.forename: Jinshao
authors.initials: J
authors.lastname: Ye
authors.affiliation: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, College of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dang
authors.affiliation: Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Zhichen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D001673:Biodegradation, Environmental; D058675:Brevibacillus / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D011721:Pyrenes; D014874:Water Pollutants, Chemical; C030984:pyrene
keywords: Biodegradation; Biosorption; Brevibacillus brevis; Cellular responses; Flow cytometry; Pyrene