title: Lentivirus-mediated knockdown of rhomboid domain containing 1 inhibits colorectal cancer cell growth.
abstract: Rhomboid domain containing 1 (RHBDD1), is a member of the rhomboid protease family, which has a pivotal role in the progression of numerous severe malignancies. However, its role in colorectal carcinoma (CRC) remains to be elucidated. In the present study, RHBDD1 was shown to be widely expressed in CRC cell lines. Lentivirus-mediated RNA interference was employed to knockdown RHBDD1 expression in RKO CRC cells. Functional analyses indicated that depletion of RHBDD1 expression resulted in significantly reduced CRC cell proliferation and colony formation, and induced a G0/G1 phase cell cycle arrest. The findings of the present study suggest that RHBDD1 may contribute to CRC tumorigenesis and serve as a potential therapeutic target in human CRC.
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of General Surgery, People's Hospital of Dayao, Yunnan 675400, P.R. China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ge
authors.affiliation: Department of Thoracic Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhong
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D059585:G1 Phase Cell Cycle Checkpoints; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C534711:RHBDD1 protein, human; D012697:Serine Endopeptidases