title: GSTM1, GSTT1, and NQO1 polymorphisms in cervical carcinogenesis.
abstract: The aim of the study is to investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1, and NQO1 c.609C>T (rs1800566) genetic polymorphisms in cervical carcinogenesis. GSTM1, GSTT1, and NQO1 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 192 cervical smear in exfoliated cervical cell samples using polymerase chain reaction (PCR) system and real-time polymerase chain reaction (PCR) system. The 19 patients with high-grade squamous intraepithelial lesion had statistically higher frequency of null GSTT1 genotype than 9 with low-grade squamous intraepithelial lesion (LSIL) among the 67 patients with high-risk HPV (P = 0.024). The 24 patients with HSIL had also statistically higher frequency of NQO1 (CT+TT) genotype than 14 with LSIL among the 67 patients with high-risk HPV (P = 0.024). GSTT1 null and NQO1 genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population.
authors.affiliation: Department of Medical Technology, Kobe Tokiwa University, 6-2 2 chome, Ohtanicho, Nagata-ku, Kobe, Hyogo, 6530838, Japan, o-nunobiki@kobe-tokiwa.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nunobiki
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ueda
authors.forename: Hikari
authors.initials: H
authors.lastname: Akise
authors.forename: Shinji
authors.initials: S
authors.lastname: Izuma
authors.forename: Kiyo
authors.initials: K
authors.lastname: Torii
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tanaka
authors.forename: Sadamu
authors.initials: S
authors.lastname: Noda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Akashi
authors.forename: Taro
authors.initials: T
authors.lastname: Higashida
mesh_terms: D044466:Asian People; D063646:Carcinogenesis / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D060005:Genotyping Techniques / Q000379:methods; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D027383:Papillomaviridae; D011110:Polymorphism, Genetic / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: D005982:Glutathione Transferase; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; C413545:glutathione S-transferase T1