title: UDP-glucosyltransferase71c5, a major glucosyltransferase, mediates abscisic acid homeostasis in Arabidopsis.
abstract: Abscisic acid (ABA) plays a key role in plant growth and development. The effect of ABA in plants mainly depends on its concentration, which is determined by a balance between biosynthesis and catabolism of ABA. In this study, we characterize a unique UDP-glucosyltransferase (UGT), UGT71C5, which plays an important role in ABA homeostasis by glucosylating ABA to abscisic acid -: glucose ester (GE) in Arabidopsis (Arabidopsis thaliana). Biochemical analyses show that UGT71C5 glucosylates ABA in vitro and in vivo. Mutation of UGT71C5 and down-expression of UGT71C5 in Arabidopsis cause delay in seed germination and enhanced drought tolerance. In contrast, overexpression of UGT71C5 accelerates seed germination and reduces drought tolerance. Determination of the content of ABA and ABA-GE in Arabidopsis revealed that mutation in UGT71C5 and down-expression of UGT71C5 resulted in increased level of ABA and reduced level of ABA-GE, whereas overexpression of UGT71C5 resulted in reduced level of ABA and increased level of ABA-GE. Furthermore, altered levels of ABA in plants lead to changes in transcript abundance of ABA-responsive genes, correlating with the concentration of ABA regulated by UGT71C5 in Arabidopsis. Our work shows that UGT71C5 plays a major role in ABA glucosylation for ABA homeostasis.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Yan
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: De-Kuan
authors.initials: DK
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Qin
authors.identifier: http://orcid.org/0000-0003-0739-680X
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Qiujie
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Ye
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
authors.forename: Xu-Feng
authors.initials: XF
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.) yangyi528@scu.edu.cn.
authors.forename: Yi
authors.identifier: http://orcid.org/0000-0002-7437-5644
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018525:Germination; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D006706:Homeostasis; D009154:Mutation; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D000040:Abscisic Acid; D005964:Glucosyltransferases; C000604705:UGT71C5 protein, Arabidopsis