title: Effects of preventive administration of oxidized dextran on liver injury and reparative regeneration in mice infected with influenza A/H5N1 virus.
abstract: Intranasal infection of outbred male mice with influenza A/H5N1 A/goose/Krasnoozerskoye/627/05 virus led to high (85%) mortality of animals. Morphological studies of liver specimens showed destructive changes in the parenchyma (93.5% hepatocytes), caused by long persistence of the virus in the liver. The virus persistence was conjugated with activation of cellular immunity, manifesting by an increase in the counts of cells with high expression of proinflammatory cytokines (TNF-α) and lysosomal enzymes (lysozyme, cathepsin D). Injections of oxidized dextran 3 and 1 days before infection reduced mortality and 2-fold attenuated destructive changes in the liver, presumably due to prevention of virus penetration into the target cells, modulation of immune reactions, and stimulation of reparative plastic processes.
authors.affiliation: Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Shkurupy
authors.forename: O V
authors.initials: OV
authors.lastname: Potapova
authors.forename: T V
authors.initials: TV
authors.lastname: Sharkova
authors.forename: A M
authors.initials: AM
authors.lastname: Shestopalov
authors.forename: A V
authors.initials: AV
authors.lastname: Troitskii
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003911:Dextrans / Q000494:pharmacology*; D006652:Histological Techniques; D007150:Immunohistochemistry; D053124:Influenza A Virus, H5N1 Subtype; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000502:physiology*; D008297:Male; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D009929:Organ Size; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology
chemical_list: D016207:Cytokines; D003911:Dextrans; D009113:Muramidase; D002402:Cathepsin D