title: [Optimization of the fermentation conditions for 5-keto-D-gluconic acid production].
abstract: Gluconobacter oxydans converts glucose to gluconic acid and subsequently to 5-keto-D-gluconic acid (5-KGA), a precursor of industrially important L(+)-tartaric acid. To increase the yield of 5-KGA, fermentation conditions of 5-KGA production was optimized. Under the optimum medium and culture conditions in the shake flask, the highest 5-KGA production reached 19.7 g/L, increased by 43.8% after optimization. In a 5-L bioreactor, the pH was controlled at 5.5 and dissolved oxygen (DO) at 15%, 5-KGA production reached 46.0 g/L, raised at least 1.3 times than in the shake flask. Glucose feeding fermentation process was further developed, and the highest 5-KGA production of 75.5 g/L with 70% of yield was obtained, 32.0% higher than the highest reported value. Therefore, this newly developed fermentation process provided a practical and effective alternative for the commercial production of 5-KGA, and further of L(+)-tartaric acid.
authors.forename: Boyi
authors.initials: B
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Pan
authors.forename: Weirong
authors.initials: W
authors.lastname: Sun
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Cheng
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Xie
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D013644:Tartrates / Q000378:metabolism
chemical_list: D005942:Gluconates; D013644:Tartrates; D005947:Glucose; C030691:gluconic acid; C029768:tartaric acid