title: DNA electrochemical biosensor for metallic drugs at physiological conditions.
abstract: Entrapment of dsSS-DNA into the polypyrrole-polyvinyl sulphonate (dsSS-DNA-PPy-PVS) film over indium-tin-oxide (ITO) coated glass has been designed to detect titanium and platinum drugs, titanocene dichloride and cisplatin. The disposable dsSS-DNA-PPy-PVS/ITO biosensor was characterized by cyclic voltammetry, attenuated total reflectance Infrared spectroscopy and atomic force microscopy. Amperometric studies by cyclic voltammetry using, dsSS-DNA-PPy PVS/ITO biosensor, demonstrated the ability of this biosensor to detect these metallic drugs in millimolar concentration by monitoring the decrease of the guanine oxidation signal as a result of the DNA damage. The concentration range detected for titanocene dichloride is 0.25 to 1.5 mM and for cisplatin is 0.06 to 1.0 mM.
authors.affiliation: University of Puerto Rico, Department of Chemical Engineering, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Angel J
authors.initials: AJ
authors.lastname: Santiago-Lopez
authors.affiliation: University of Puerto Rico, Department of Chemistry, PO Box 9019 Mayagüez, PR 00681.
authors.forename: José L
authors.initials: JL
authors.lastname: Vera
authors.affiliation: University of Puerto Rico, Department of Chemistry, PO Box 9019 Mayagüez, PR 00681.
authors.forename: Enrique
authors.initials: E
authors.lastname: Meléndez
keywords: DNA biosensor; cisplatin; electrochemical detection; electrochemical entrapment; titanocene dichloride