title: Lactoferrin Combined with Retinoic Acid Stimulates B1 Cells to Express IgA Isotype and Gut-homing Molecules.
abstract: It is well established that TGF-β1 and retinoic acid (RA) cause IgA isotype switching in mice. We recently found that lactoferrin (LF) also has an activity of IgA isotype switching in spleen B cells. The present study explored the effect of LF on the Ig production by mouse peritoneal B cells. LF, like TGF-β1, substantially increased IgA production in peritoneal B1 cells but little in peritoneal B2 cells. In contrast, LF increased IgG2b production in peritoneal B2 cells much more strongly than in peritoneal B1 cells. LF in combination with RA further enhanced the IgA production and, interestingly, this enhancement was restricted to IgA isotype and B1 cells. Similarly, the combination of the two molecules also led to expression of gut homing molecules α4β7 and CCR9 on peritoneal B1 cells, but not on peritoneal B2 cells. Thus, these results indicate that LF and RA can contribute to gut IgA response through stimulating IgA isotype switching and expression of gut-homing molecules in peritoneal B1 cells.
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Jin
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Hyeon-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Goo-Young
authors.initials: GY
authors.lastname: Seo
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Young-Saeng
authors.initials: YS
authors.lastname: Jang
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: An
authors.affiliation: Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea.
authors.forename: Seok-Rae
authors.initials: SR
authors.lastname: Park
authors.affiliation: Department of Animal Life and Environmental Science, College of Agriculture and Life Science, Hankyong National University, Anseong 456-749, Korea.
authors.forename: Woan-Sub
authors.initials: WS
authors.lastname: Kim
authors.affiliation: Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Pyeung-Hyeun
authors.initials: PH
authors.lastname: Kim
keywords: Gut-homing molecule; IgA; Lactoferrin; Peritoneal B1 cell; Retinoic acid