title: Study on Biological Effects of La(3+) on Rat Liver Mitochondria by Microcalorimetric and Spectroscopic Methods.
abstract: The effects of lanthanum on heat production of mitochondria isolated from Wistar rat liver were investigated with microcalorimetry; simultaneously, the effects on mitochondrial swelling and membrane potential (Δψ) were determined by spectroscopic methods. La(3+) showed only inhibitory action on mitochondrial energy turnover with IC50 being 55.8 μmol L(-1). In the spectroscopic experiments, La(3+), like Ca(2+), induced rat liver mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor, cyclosporine A (CsA). The induction ability of La(3+) was stronger than that of Ca(2+). These results demonstrated that La(3+) had some biotoxicity effect on mitochondria; the effects of La(3+) and Ca(2+) on rat liver mitochondrial membrane permeability transition (MPT) are different, and La represents toxic action rather than Ca analogy.
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, 434023, Hubei, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Gao
authors.forename: Zhi-Jiang
authors.initials: ZJ
authors.lastname: Feng
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Ye-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002151:Calorimetry / Q000379:methods; D016572:Cyclosporine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects; D008933:Mitochondrial Swelling / Q000187:drug effects*; D010539:Permeability / Q000187:drug effects; D017208:Rats, Wistar; D013053:Spectrophotometry
chemical_list: D007166:Immunosuppressive Agents; D007811:Lanthanum; D016572:Cyclosporine; D002118:Calcium