title: High-titer lactic acid production from NaOH-pretreated corn stover by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile condition.
abstract: Lactic acid (LA) is an important chemical with various industrial applications. Non-food feedstock is commercially attractive for use in LA production; however, efficient LA fermentation from lignocellulosic biomass resulting in both high yield and titer faces technical obstacles. In this study, the thermophilic bacterium Bacillus coagulans LA204 demonstrated considerable ability to ferment glucose, xylose, and cellobiose to LA. Importantly, LA204 produces LA from several NaOH-pretreated agro stovers, with remarkably high yields through simultaneous saccharification and fermentation (SSF). A fed-batch SSF process conducted at 50°C and pH 6.0, using a cellulase concentration of 30 FPU (filter paper unit)/g stover and 10 g/L yeast extract in a 5-L bioreactor, was developed to produce LA from 14.4% (w/w) NaOH-pretreated non-sterile corn stover. LA titer, yield, and average productivity reached 97.59 g/L, 0.68 g/g stover, and 1.63 g/L/h, respectively. This study presents a feasible process for lignocellulosic LA production from abundant agro stovers.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Zhenting
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Yanxu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430070, PR China.
authors.forename: Shumiao
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Mei
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430070, PR China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430070, PR China. Electronic address: nanp@mail.hzau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Peng
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry; D013242:Sterilization; D013696:Temperature; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D019344:Lactic Acid; D012972:Sodium Hydroxide; D002244:Carbon; D008031:Lignin; D002480:Cellulase; D009584:Nitrogen
keywords: Bacillus coagulans LA204; Corn stover; High lactic acid titer; Lactic acid fermentation; Simultaneous saccharification and fermentation (SSF)