title: Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus.
abstract: OBJECTIVE
  Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/and without oropharyngeal candidiasis (OPC).
  
  
  METHODS
  A total of 100 C. albicans isolates from Iranian HIV(+)patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes.
  
  
  RESULTS
  The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence.
  
  
  CONCLUSIONS
  In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV(+) patients.
authors.affiliation: Bu Ali Sina University, Hamedan, Iran ; Research and Training Center of Imam Khomeini Hospital, Tehran, Iran.
authors.forename: Iradj Ashrafi
authors.initials: IA
authors.lastname: Tamai
authors.affiliation: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Taghi Zahraei
authors.initials: TZ
authors.lastname: Salehi
authors.affiliation: Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Aghil
authors.initials: A
authors.lastname: Sharifzadeh
authors.affiliation: Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
authors.forename: Hojjatollah
authors.initials: H
authors.lastname: Shokri
authors.affiliation: Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Khosravi
keywords: Candida albicans; Candidiasis; Genotyping; HIV+; RPS sequence