title: Successful implantation of autologous valved conduits with self-expanding stent (stent-biovalve) within the pulmonary artery in beagle dogs.
abstract: OBJECTIVE
  To evaluate the functionality of an autologous heart valve with stent (Stent-biovalve or SBV) after implantation in the pulmonic valve position in beagle dogs.
  
  
  METHODS
  Five beagle dogs.
  
  
  METHODS
  A mold with an aperture of a tri-leaflet structure was constructed from a pair of concave and convex rods to which a nitinol (NiTi) stent was mounted. This mold was embedded in a dorsal subcutaneous pouch in beagle dogs for 4 weeks. At the time of the removal, the surfaces of the molds were completely covered with connective tissues, tri-leaflet valves were formed and the NiTi stent was tightly connected to the structure.
  
  
  RESULTS
  The mean burst strength of the SBV leaflet was 2710 mmHg (range 2280-3116 mmHg), which was approximately equal to that of the native pulmonic valve leaflet. After implantation in the pulmonary position, the SBV showed good functionality as a pulmonic valve. At 84 days after implantation, the SBV was replaced with autologous fibroblasts and collagenous tissues, and showed organization similar to that of native heart valves.
  
  
  CONCLUSIONS
  Stent-Biovalves achieved good valvular function with laminar flow in the pulmonic valve position of beagle dogs.
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Marina
authors.initials: M
authors.lastname: Funayama
authors.affiliation: Department of Mechanical Systems Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Matsui
authors.affiliation: Department of Mechanical Systems Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tajikawa
authors.affiliation: College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Sasagawa
authors.affiliation: College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Saito
authors.affiliation: College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Sagishima
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Harada
authors.affiliation: Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Uchida
authors.affiliation: Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Shinoda
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Iwai
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan. Electronic address: uechi.masami@cardiovets.jp.
authors.forename: Masami
authors.initials: M
authors.lastname: Uechi
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000379:methods*; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D003238:Connective Tissue; D004285:Dogs; D008422:Materials Testing; D015607:Stents / Q000662:veterinary*
keywords: Heart valve replacement; Pulmonic stenosis; Regenerative medicine; Self-expandable stent; Tissue engineering