title: Trade-off between thermal tolerance and insecticide resistance in Plutella xylostella.
abstract: Fitness costs associated with resistance to insecticides have been well documented, usually at normal temperature conditions, in many insect species. In this study, using chlorpyrifos-resistant homozygote (RR) and chlorpyrifos-susceptible homozygote (SS) of resistance ace1 allele of Plutella xylostella (DBM), we confirmed firstly that high temperature experience in pupal stage influenced phenotype of wing venation in insecticide-resistant and insecticide-susceptible Plutella xylostella, and SS DBM showed significantly higher thermal tolerance and lower damages of wing veins under heat stress than RR DBM. As compared to SS DBM, RR DBM displayed significantly lower AChE sensitivity to chlorpyrifos, higher basal GSTs activity and P450 production at 25°C, but higher inhibitions on the enzyme activities and P450 production as well as reduced resistance to chlorpyrifos under heat stress. Furthermore, RR DBM displayed significantly higher basal expressions of hsp69s, hsp72s, hsp20,hsp90,Apaf-1, and caspase-7 at 25°C, but lower induced expressions of hsps and higher induced expressions of Apaf-1,caspase-9, and caspase-7 under heat stress. These results suggest that fitness costs of chlorpyrifos resistance in DBM may partly attribute to excess consumption of energy caused by over production of detoxification enzymes and hsps when the proteins are less demanded at conducive environments but reduced expressions when they are highly demanded by the insects to combat environmental stresses, or to excess expressions of apoptotic genes under heat stress, which results in higher apoptosis. The evolutionary and ecological implications of these findings at global warming are discussed.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Lin Jie
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Zhao Li
authors.initials: ZL
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Kuan Fu
authors.initials: KF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Hua Mei
authors.initials: HM
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University Fuzhou, 350002, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
keywords: Caspase; Plutella xylostella; fitness cost of chlorpyrifos resistance; heat shock proteins; resistance-related enzymes; thermal tolerance