title: Expression and mutational analysis of DinB-like protein DR0053 in Deinococcus radiodurans.
abstract: In order to understand the mechanism governing radiation resistance in Deinococcus radiodurans, current efforts are aimed at identifying potential candidates from a large repertoire of unique Deinococcal genes and protein families. DR0053 belongs to the DinB/YfiT protein family, which is an over-represented protein family in D. radiodurans. We observed that dr0053 transcript levels were highly induced in response to gamma radiation (γ-radiation) and mitomycin C (MMC) exposure depending on PprI, RecA and the DrtR/S two-component signal transduction system. Protein profiles demonstrated that DR0053 is a highly induced protein in cultures exposed to 10 kGy γ-radiation. We were able to determine the transcriptional start site of dr0053, which was induced upon irradiation, and to assign the 133-bp promoter region of dr0053 as essential for radiation responsiveness through primer extension and promoter deletion analyses. A dr0053 mutant strain displayed sensitivity to γ-radiation and MMC exposure, but not hydrogen peroxide, suggesting that DR0053 helps cells recover from DNA damage. Bioinformatic analyses revealed that DR0053 is similar to the Bacillus subtilis protein YjoA, which is a substrate of bacterial protein-tyrosine kinases. Taken together, the DNA damage-inducible (din) gene dr0053 may be regulated at the transcriptional and post-translational levels.
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Deepti
authors.initials: D
authors.lastname: Appukuttan
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Ho Seong
authors.initials: HS
authors.lastname: Seo
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Sunwook
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Sunghun
authors.initials: S
authors.lastname: Im
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Minho
authors.initials: M
authors.lastname: Joe
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Dusup
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jungjoon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods; D034301:Deinococcus / Q000187:drug effects / Q000235:genetics*; D005720:Gamma Rays; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D016685:Mitomycin; D006861:Hydrogen Peroxide; D011505:Protein-Tyrosine Kinases