title: [An expedient synthesis of fluorescent labeled ceramide-1-phosphate analogues].
abstract: A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a- diaza-s-indacene-8-yl (Me4-BODIPY) fluorophore at o-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus; the enzymatic yield has been raised to 50-70% by appliance of organic solvent in the incubation medium.
authors.forename: I A
authors.initials: IA
authors.lastname: Boldyrev
authors.forename: R E
authors.initials: RE
authors.lastname: Braun
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Molotkovskiĭ
mesh_terms: D001896:Boron Compounds / Q000737:chemistry; D002518:Ceramides / Q000138:chemical synthesis* / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis; D010713:Phosphatidylcholines / Q000737:chemistry; D010739:Phospholipase D / Q000737:chemistry*; D013109:Sphingomyelins / Q000138:chemical synthesis / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology
chemical_list: C525900:(me4-BODIPY-8)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacen-8-yl; D001896:Boron Compounds; D002518:Ceramides; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D013109:Sphingomyelins; C065576:ceramide 1-phosphate; D010739:Phospholipase D