title: Ribifolin, an orbitide from Jatropha ribifolia, and its potential antimalarial activity.
abstract: A new orbitide named ribifolin was isolated and characterized from Jatropha ribifolia using mass spectrometry, NMR spectroscopy, quantitative amino acid analysis, molecular dynamics/simulated annealing, and Raman optical activity measurements and calculations. Ribifolin (1) and its linear form (1a) were synthesized by solid-phase peptide synthesis, followed by evaluation of its antiplasmodial and cytotoxicity activities. Compound 1 was moderately effective (IC50 = 42 μM) against the Plasmodium falciparum strain 3D7.
authors.affiliation: †Institute of Chemistry, São Paulo State University-UNESP, 14800-060, Araraquara, São Paulo, Brazil.
authors.forename: Meri Emili F
authors.initials: ME
authors.lastname: Pinto
authors.affiliation: †Institute of Chemistry, São Paulo State University-UNESP, 14800-060, Araraquara, São Paulo, Brazil.|‡Manchester Institute of Biotechnology and Faculty of Life Sciences, The University of Manchester, M1 7DN, Manchester, United Kingdom.
authors.forename: João M
authors.initials: JM
authors.lastname: Batista
authors.affiliation: §School of Biomedical Sciences, The University of Queensland, 4072, St. Lucia, Queensland, Australia.|⊥Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Koehbach
authors.affiliation: ∥Departament of Physiology, Institute of Biosciences, The University of São Paulo-USP, 05508-900, São Paulo, Brazil.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Gaur
authors.affiliation: ∥Departament of Physiology, Institute of Biosciences, The University of São Paulo-USP, 05508-900, São Paulo, Brazil.
authors.forename: Abhinay
authors.initials: A
authors.lastname: Sharma
authors.affiliation: ∥Departament of Physiology, Institute of Biosciences, The University of São Paulo-USP, 05508-900, São Paulo, Brazil.
authors.forename: Myna
authors.initials: M
authors.lastname: Nakabashi
authors.affiliation: †Institute of Chemistry, São Paulo State University-UNESP, 14800-060, Araraquara, São Paulo, Brazil.
authors.forename: Eduardo Maffud
authors.initials: EM
authors.lastname: Cilli
authors.affiliation: #Center of Biotechnology, Federal University of Rio Grande do Sul-UFRGS, 91500-970, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Guilherme M
authors.initials: GM
authors.lastname: Giesel
authors.affiliation: #Center of Biotechnology, Federal University of Rio Grande do Sul-UFRGS, 91500-970, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Hugo
authors.initials: H
authors.lastname: Verli
authors.affiliation: ⊥Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.
authors.forename: Christian W
authors.initials: CW
authors.lastname: Gruber
authors.affiliation: ‡Manchester Institute of Biotechnology and Faculty of Life Sciences, The University of Manchester, M1 7DN, Manchester, United Kingdom.
authors.forename: Ewan W
authors.initials: EW
authors.lastname: Blanch
authors.affiliation: □Laboratory of Pharmaceutical Technology, Federal University of Paraíba-UFPB, 58051-970, João Pessoa, Paraíba, Brazil.
authors.forename: Joseam F
authors.initials: JF
authors.lastname: Tavares
authors.affiliation: □Laboratory of Pharmaceutical Technology, Federal University of Paraíba-UFPB, 58051-970, João Pessoa, Paraíba, Brazil.
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: da Silva
authors.affiliation: ∥Departament of Physiology, Institute of Biosciences, The University of São Paulo-USP, 05508-900, São Paulo, Brazil.
authors.forename: Celia R S
authors.initials: CR
authors.lastname: Garcia
authors.affiliation: †Institute of Chemistry, São Paulo State University-UNESP, 14800-060, Araraquara, São Paulo, Brazil.
authors.forename: Vanderlan S
authors.initials: VS
authors.lastname: Bolzani
mesh_terms: D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D031286:Jatropha / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D021261:Parasitic Sensitivity Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000962:Antimalarials; D010456:Peptides, Cyclic; D010936:Plant Extracts; C000598545:ribifolin