title: Synthesis, Characterization, Antioxidant Status, and Toxicity Study of Vanadium-Rutin Complex in Balb/c Mice.
abstract: A new trend was developed for the formation of a complex between vanadium and flavonoid derivatives in order to increase the intestinal absorption and to reduce the toxicity of vanadium compounds. The vanadium-rutin complex was characterized by several spectroscopic techniques like ultraviolet (UV)-visible, Fourier transform infrared (FTIR), NMR, mass spectrometry, and microscopic evaluation by scanning electron microscopy. The mononuclear complex was formed by the interaction between vanadium and rutin with 1:2 metal to ligand stoichiometry. Antioxidant activity of the complex was evaluated by 1,1-diphenyl-2 picrylhydrazyl, ferric-reducing power, and 2,2'-azin-obis 3-ethylbenzothiazoline-6-sulphonic acid methods. It was shown that radical scavenging activity and ferric-reducing potential of free rutin was lower as compared with vanadium-rutin complex. The study was also investigated for oral acute toxicity and 28 days repeated oral subacute toxicity study of vanadium-rutin complex in balb/c mice. The vanadium-rutin complex showed mortality at a dose of 120 mg/kg in the balb/c mice. In 28 days repeated oral toxicity study, vanadium-rutin complex was administered to both sex of balb/c mice at dose levels of 90, 45, and 20 ppm, respectively. In addition, subacute toxicity study of vanadium-rutin complex (at 90 ppm dose level) showed increase levels of white blood cell (WBC), total bilirubin, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatinine, and blood urea nitrogen and decrease level of total protein (TP) as compared with control group. Histopathological study of vanadium-rutin showed structural alteration in the liver, kidney, and stomach at 90 ppm dose level. No observed toxic level of vanadium-rutin complex at 20 ppm dose level could be good for further study.
authors.affiliation: Department of Pharmaceutical Technology, NSHM Knowledge Campus-Kolkata, 124 B.L. Saha Road, Kolkata, 700053, West Bengal, India, souvikroy35@gmail.com.
authors.forename: Souvik
authors.initials: S
authors.lastname: Roy
authors.forename: Sumana
authors.initials: S
authors.lastname: Majumdar
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
authors.forename: Nilanjan
authors.initials: N
authors.lastname: Ghosh
authors.forename: Subhadip
authors.initials: S
authors.lastname: Manna
authors.forename: Tania
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Sougato
authors.initials: S
authors.lastname: Mallick
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012431:Rutin / Q000737:chemistry*; D013270:Stomach / Q000187:drug effects; D014639:Vanadium / Q000737:chemistry*; D017968:Vanadium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D017968:Vanadium Compounds; D014639:Vanadium; D012431:Rutin