title: TGFβ3 secretion by three-dimensional cultures of human dental apical papilla mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) can be isolated from dental tissues, such as pulp and periodontal ligament; the dental apical papilla (DAP) is a less-studied MSC source. These dental-derived MSCs are of great interest because of their potential as an accessible source for cell-based therapies and tissue-engineering (TE) approaches. Much of the interest regarding MSCs relies on the trophic-mediated repair and regenerative effects observed when they are implanted. TGFβ3 is a key growth factor involved in tissue regeneration and scarless tissue repair. We hypothesized that human DAP-derived MSCs (hSCAPs) can produce and secrete TGFβ3 in response to micro-environmental cues. For this, we encapsulated hSCAPs in different types of matrix and evaluated TGFβ3 secretion. We found that dynamic changes of cell-matrix interactions and mechanical stress that cells sense during the transition from a monolayer culture (two-dimensional, 2D) towards a three-dimensional (3D) culture condition, rather than the different chemical composition of the scaffolds, may trigger the TGFβ3 secretion, while monolayer cultures showed almost 10-fold less secretion of TGFβ3. The study of these interactions is provided as a cornerstone in designing future strategies in TE and cell therapy that are more efficient and effective for repair/regeneration of damaged tissues. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Centro de Biotecnología, Universidad Técnica Federico Santa María, Valparaíso, Chile.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Somoza
authors.affiliation: Centro de Biotecnología, Universidad Técnica Federico Santa María, Valparaíso, Chile.
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Acevedo
authors.affiliation: Centro de Biotecnología, Universidad Técnica Federico Santa María, Valparaíso, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Albornoz
authors.affiliation: Laboratorio de Inmunología, Universidad de los Andes, Santiago, Chile.
authors.forename: Patricia
authors.initials: P
authors.lastname: Luz-Crawford
authors.affiliation: Laboratorio de Inmunología, Universidad de los Andes, Santiago, Chile.
authors.forename: Flavio
authors.initials: F
authors.lastname: Carrión
authors.affiliation: Centro de Biotecnología, Universidad Técnica Federico Santa María, Valparaíso, Chile.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Young
authors.affiliation: Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Chile.
authors.forename: Caroline
authors.initials: C
authors.lastname: Weinstein-Oppenheimer
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology*; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008954:Models, Biological; D025341:Principal Component Analysis; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3
keywords: 3D culture; SCAP; TGFβ3; alginate; fibrin; mesenchymal stem cells