title: Placental expression of eNOS, iNOS and the major protein components of caveolae in women with pre-eclampsia.
abstract: Caveolae regulate many cardiovascular functions and thus could be of interest in relation to pre-eclampsia, a pregnancy specific disorder characterised by hypertension and proteinuria. We examined placental mRNA and protein expression/localisation of the caveolae components Caveolin 1-3, Cavin 1-4 as well as eNOS/iNOS in normotensive control (n = 24) and pre-eclamptic pregnancies (n = 19). Placental mRNA expression of caveolin-1, cavin 1-3, was lower and eNOS expression was increased in pre-eclampsia (P < 0.05 for all). Additionally Caveolin-1 protein expression was also reduced in pre-eclampsia (P = 0.007); this could be an adaptive response in pre-eclampsia, possibly to attenuate the oxidative stress/inflammation.
authors.affiliation: Division of Women's Health, King's College London, Women's Health Academic Centre, UK; Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, UK.
authors.forename: K
authors.initials: K
authors.lastname: Smith-Jackson
authors.affiliation: Division of Women's Health, King's College London, Women's Health Academic Centre, UK; Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, UK; Laboratory of Nephrology - IPB, School of Medicine, PUCRS, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: Hentschke
authors.affiliation: Laboratory of Nephrology - IPB, School of Medicine, PUCRS, Brazil.
authors.forename: C E
authors.initials: CE
authors.lastname: Poli-de-Figueiredo
authors.affiliation: Laboratory of Nephrology - IPB, School of Medicine, PUCRS, Brazil.
authors.forename: B E
authors.initials: BE
authors.lastname: Pinheiro da Costa
authors.affiliation: Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, UK.
authors.forename: L O
authors.initials: LO
authors.lastname: Kurlak
authors.affiliation: Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, UK.
authors.forename: F
authors.initials: F
authors.lastname: Broughton Pipkin
authors.affiliation: Division of Diabetes and Nutritional Sciences, Diabetes Research Group, King's College London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Czajka
authors.affiliation: Division of Women's Health, King's College London, Women's Health Academic Centre, UK; Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, UK. Electronic address: hiten.mistry@kcl.ac.uk.
authors.forename: H D
authors.initials: HD
authors.lastname: Mistry
mesh_terms: D016022:Case-Control Studies; D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D051242:Caveolin 1; C496319:NOS2 protein, human; C496314:NOS3 protein, human; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
keywords: Caveolin; Cavin; Hypertension; Placenta; Pre-eclampsia