title: Chronodependent effect of interleukin-2 on mouse spleen cells in the model of cyclophosphamide immunosuppression.
abstract: We studied the chronodependent effect of IL-2 in the experimental model of immunodeficiency, cyclophosphamide-induced immunosuppression in mice. IL-2 in a dose of 100 U/ mouse was administered at 10.00 and 16.00 for 3 days after injection of cyclophosphamide. In contrast to the morning treatment with the cytokine, evening administration produced antiapoptotic effect on splenocytes and stimulated proliferation to a greater extent. This was accompanied by an increase in the number of CD4(+), CD25(+) and CD4(+)25(+) cells in the spleen to a level of intact mice. More pronounced effect of the evening mode of IL-2 administration on the proliferation and subpopulation composition of mouse spleen cells in the studied model can be associated with high blood level of CD25(+) cells at this time of the day.
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk, Russia, anna_v_s@mail.ru.
authors.forename: A V
authors.initials: AV
authors.lastname: Shurlygina
authors.forename: E V
authors.initials: EV
authors.lastname: Mel'nikova
authors.forename: V A
authors.initials: VA
authors.lastname: Trufakin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003520:Cyclophosphamide / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate; D007153:Immunologic Deficiency Syndromes / Q000139:chemically induced / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D003520:Cyclophosphamide; D016650:Fluorescein-5-isothiocyanate