title: Mulberry (Morus L.) methionine sulfoxide rreductase gene cloning, sequence analysis, and expression in plant development and stress response.
abstract: Methionine sulfoxide reductase plays a regulatory role in plant growth and development, especially in scavenging reactive oxygen species by restoration of the oxidation of methionine in protein. A full-length cDNA sequence encoding methionine sulfoxide reductase (MSR) from mulberry, which we designated MMSR, was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MMSR is 810 bp long, encoding 194 amino acids with a predicted molecular weight of 21.6 kDa and an isoelectric point of 6.78. The expression level of the MMSR gene under conditions of drought and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.
authors.affiliation: College of Biological and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Zhaoyue
authors.initials: Z
authors.lastname: Liu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Rongjun
authors.initials: R
authors.lastname: Fang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhao
authors.forename: Long
authors.initials: L
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D051150:Methionine Sulfoxide Reductases / Q000096:biosynthesis / Q000235:genetics*; D031625:Morus / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D017421:Sequence Analysis; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase