title: Bioassay-guided isolation of antibacterial constituents from Diospyros lotus roots.
abstract: The aim of this study was to explore the extract/fractions and compounds of Diospyros lotus against various Gram-positive and Gram-negative bacteria strain. The results showed marked susceptibility of extract and its fractions against test pathogens. Among them, chloroform fraction was most dominant and effective against all tested bacteria. The chloroform fraction was subjected to column chromatography which led to the isolation of lupeol (1), 7-methyljuglone (2), β-sitosterol (3), stigmasterol (4), betulinic acid (5), diospyrin (6) and 8-hydroxyisodiospyrin (7). Among the isolated compounds, betulinic acid (5) showed significant activity against most of the tested pathogen. In conclusion, our study validated the traditional uses of the plant in the treatment of infectious diseases which was also strongly supported by the isolated compound, betulinic acid (5).
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , K.P.K Peshawar , Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , K.P.K Peshawar , Pakistan.
authors.forename: Ghias
authors.initials: G
authors.lastname: Uddin
authors.affiliation: b Department of Pharmacy , Abdul Wali Khan University , Mardan 23200 , Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , K.P.K Peshawar , Pakistan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arfan
authors.affiliation: c H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi , Karachi - 75270 , Pakistan.
authors.forename: Bina S
authors.initials: BS
authors.lastname: Siddiqui
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002725:Chloroform; D029788:Diospyros / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012997:Solvents; D002725:Chloroform
keywords: Diospyros lotus; Ebenaceae; antibacterial activity; phytoconstituents