title: Rootin, a compound that inhibits root development through modulating PIN-mediated auxin distribution.
abstract: Plant roots anchor the plant to the soil and absorb water and nutrients for growth. Understanding the molecular mechanisms regulating root development is essential for improving plant survival and agricultural productivity. Extensive molecular genetic studies have provided important information on crucial components for the root development control over the last few decades. However, it is becoming difficult to identify new regulatory components in root development due to the functional redundancy and lethality of genes involved in root development. In this study, we performed a chemical genetic screen to identify novel synthetic compounds that regulate root development in Arabidopsis seedlings. The screen yielded a root growth inhibitor designated as 'rootin', which inhibited Arabidopsis root development by modulating cell division and elongation, but did not significantly affect shoot development. Transcript analysis of phytohormone marker genes revealed that rootin preferentially altered the expression of auxin-regulated genes. Furthermore, rootin reduced the accumulation of PIN1, PIN3, and PIN7 proteins, and affected the auxin distribution in roots, which consequently may lead to the observed defects in root development. Our results suggest that rootin could be utilized to unravel the mechanisms underlying root development and to investigate dynamic changes in PIN-mediated auxin distribution.
authors.affiliation: Department of Life Sciences, POSTECH, Hyojadong, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Suyeong
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, NUS & Singapore Bioimaging Consortium, A*STAR, Singapore 117543, Singapore.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Integrative Bioscience and Biotechnology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
authors.forename: Hyunmo
authors.initials: H
authors.lastname: Choi
authors.affiliation: Department of Life Sciences, POSTECH, Hyojadong, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Hyunmin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, POSTECH, Hyojadong, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Ilhwan
authors.initials: I
authors.lastname: Lee
authors.affiliation: Department of Integrative Bioscience and Biotechnology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Soh
authors.affiliation: Center for Plant Aging Research, Institute for Basic Science (IBS), Republic of Korea; Department of New Biology, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Hong Gil
authors.initials: HG
authors.lastname: Nam
authors.affiliation: Department of Chemistry, NUS & Singapore Bioimaging Consortium, A*STAR, Singapore 117543, Singapore. Electronic address: chmcyt@nus.edu.sg.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Department of New Biology, DGIST, Daegu 711-873, Republic of Korea. Electronic address: polim@dgist.ac.kr.
authors.forename: Pyung Ok
authors.initials: PO
authors.lastname: Lim
authors.affiliation: Department of New Biology, DGIST, Daegu 711-873, Republic of Korea. Electronic address: hrwoo@dgist.ac.kr.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Woo
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators
keywords: Abundance of PIN proteins; Auxin transporters; Chemical genetics; Root development