title: Hematopoietic stem and progenitor cells can be enriched by implanting biomaterial into spatium intermusculare.
abstract: Hematopoietic stem and progenitor cells (HSPCs) have been used successfully to treat patients with cancer and disorders of the blood and immune systems. In this study, we tried to enrich HSPCs by implanting biomaterials into the spatium intermusculare of mice hind limbs. Gelatine sponges were implanted into the spatium intermusculare of mice and then retrieved after 12 days. The presence of HSPCs in the migrating cells (MCs) was detected by phenotypically probing with CD34(+)Sca-1(+) and functionally confirming the presence of using colony-forming cell assay and assessing the long-term reconstitution ability. The frequency of CD34(+), Sca-1(+), and CD34(+)Sca-1(+) cells and colony formation unit in the MCs was much higher than that in the bone marrow (BM). Moreover, transplanted MCs were able to home to BM, muscle, and spleen, which induced an efficient long-term hematopoietic reconstitution in vivo. In addition, HSPCs within the MCs originated from the BM. Furthermore, the administration of G-CSF greatly reduced the time of implantation, and increased the number of MCs and frequency of HSPCs in the MCs. These data provide compelling evidence that HSPCs can be enriched by implanting biomaterial into spatium intermusculare. Implantation of biomaterial may be seen as the first step to a proof of their applicability to clinical practice in enriching HSPCs.
authors.affiliation: Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China ; Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Modern Medicine and Technology of Shandong Province, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Key Laboratory for Virology of Shandong Province, Shandong Medicinal Biotechnology Centre, Shandong Academy of Medical Sciences, 18877 Jingshi Road, Jinan 250000, China.
authors.forename: Jia-Bei
authors.initials: JB
authors.lastname: Tong
authors.affiliation: Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Modern Medicine and Technology of Shandong Province, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Key Laboratory for Virology of Shandong Province, Shandong Medicinal Biotechnology Centre, Shandong Academy of Medical Sciences, 18877 Jingshi Road, Jinan 250000, China ; Department of Technology, Beijing Jingmeng Stem Cell Technology Co., Ltd., 5-2 Shangdi East Road, Beijing 100085, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China.
authors.forename: Ge-Liuchang
authors.initials: GL
authors.lastname: Jia
authors.affiliation: Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China.
authors.forename: Kui-Jun
authors.initials: KJ
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Modern Medicine and Technology of Shandong Province, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Key Laboratory for Virology of Shandong Province, Shandong Medicinal Biotechnology Centre, Shandong Academy of Medical Sciences, 18877 Jingshi Road, Jinan 250000, China.
authors.forename: Shi-Li
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing 100050, China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D003114:Colony-Forming Units Assay / Q000379:methods; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D001672:Biocompatible Materials; D016179:Granulocyte Colony-Stimulating Factor