title: Serological diagnosis of feline coronavirus infection by immunochromatographic test.
abstract: The immunochromatographic assay (ICA) is a simple antibody-antigen detection method, the results of which can be rapidly obtained at a low cost. We designed an ICA to detect anti-feline coronavirus (FCoV) antibodies. A colloidal gold-labeled recombinant FCoV nucleocapsid protein (rNP) is used as a conjugate. The Protein A and affinity-purified cat anti-FCoV IgG are blotted on the test line and the control line, respectively, of the nitrocellulose membrane. The specific detection of anti-FCoV antibodies was possible in all heparin-anticoagulated plasma, serum, whole blood, and ascitic fluid samples from anti-FCoV antibody positive cats, and nonspecific reaction was not noted in samples from anti-FCoV antibody negative cats.
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1, Towada, Aomori, 034-8628, Japan, takanot@vmas.kitasato-u.ac.jp.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D002846:Chromatography, Affinity; D000086462:Coronavirus Nucleocapsid Proteins; D016765:Coronavirus, Feline / Q000276:immunology*; D016766:Feline Infectious Peritonitis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D019590:Nucleocapsid Proteins / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins