title: Bestatin induces specific changes in Trypanosoma cruzi dipeptide pool.
abstract: Proteases and peptidases in Trypanosoma cruzi are considered potential targets for antichagasic chemotherapy. We monitored changes in low-mass metabolites in T. cruzi epimastigotes treated with bestatin, a dipeptide metalloaminopeptidase inhibitor. After treatment, multiple dipeptides were shown to be increased, confirming in situ inhibition of the leucine aminopeptidase of T. cruzi (LAPTc) and probably other peptidases.
authors.affiliation: Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay Laboratorio de Microbiología Aplicada y Biotecnología, Instituto de Investigaciones en Biodiversidad y Medio Ambiente (CONICET), Universidad Nacional del Comahue, San Carlos de Bariloche, Río Negro, Argentina atrochine@gmail.com robello@pasteur.edu.uy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Trochine
authors.affiliation: The Wellcome Trust Centre for Molecular Parasitology, Institute for Infection, Immunity and Inflammation and Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Creek
authors.affiliation: Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay.
authors.forename: Paula
authors.initials: P
authors.lastname: Faral-Tello
authors.affiliation: The Wellcome Trust Centre for Molecular Parasitology, Institute for Infection, Immunity and Inflammation and Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Barrett
authors.affiliation: Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay atrochine@gmail.com robello@pasteur.edu.uy.
authors.forename: Carlos
authors.initials: C
authors.lastname: Robello
mesh_terms: D000626:Aminopeptidases / Q000037:antagonists & inhibitors; D004151:Dipeptides / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D000626:Aminopeptidases; D007930:Leucine; C012211:ubenimex