title: Scaffold materials from glycosylated and PEGylated bovine serum albumin.
abstract: Bovine serum albumin has been PEGylated and glycosylated to create mimetic materials for the extracellular matrix (ECM) with potential tissue engineering applications. Different surfaces for cell adhesion were achieved by crosslinking the initial albumin product and forming either a coating or a sponge-like three-dimensional morphology to mimic the mesh structure of natural ECM. The biocompatibility of the albumin matrix with mammalian cells was evaluated using cell culture assays with NIH 3T3 cells. The results indicated that glycoprotein composition and specific morphology of the assembly can improve the cell growth environment. These ECM mimetic structures might eventually be considered to serve as alternatives for the more expensive collagen and elastin based ECM substances currently in use in tissue engineering.
authors.affiliation: Department of Polymer and Fiber Engineering, Auburn University, Auburn, Alabama, 36849.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering, Auburn University, Auburn, Alabama, 36849.
authors.forename: Allan E
authors.initials: AE
authors.lastname: David
authors.affiliation: Department of Chemical Engineering, Auburn University, Auburn, Alabama, 36849.
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama, 36849.
authors.forename: Yonnie
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Polymer and Fiber Engineering, Auburn University, Auburn, Alabama, 36849.
authors.forename: Gisela
authors.initials: G
authors.lastname: Buschle-Diller
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D005109:Extracellular Matrix / Q000737:chemistry; D006031:Glycosylation; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols
keywords: BSA; biocompatibility; biomaterials; cell adhesion; glycosylation