title: Evaluation of the microcirculation in a rabbit hemorrhagic shock model using laser Doppler imaging.
abstract: The aim of this study is to evaluate the feasibility of Laser Doppler imaging (LDI) for noninvasive and dynamic assessment of hemorrhagic shock in a rabbit model. A rabbit model of hemorrhagic shock was generated and LDI of the microcirculation in the rabbit ears was performed before and at 0, 30, 60, and 90 min after hemorrhage. The CCD (Charge Coupled Device) image of the ears, the mean arterial pressure (MAP) and the heart rate (HR) were monitored. The mean LDI flux was calculated. The HR of rabbits was significantly (p < 0.05) elevated and the MAP was decreased after hemorrhage, compared to the pre-hemorrhage level. Within the initial 30 min after hemorrhage, the perfusion flux lineally dropped down. In contrast, the MAP values did not differ significantly between the time points of 0 and 30 after hemorrhage (p > 0.05). Both the flux numbers and the red-to-blue color changes on LDI imaging showed the reduction of the microcirculation. LDI imaging is a noninvasive and non-contact approach to evaluate the microcirculation and may offer benefits in the diagnosis and treatment of hemorrhage shock. Further studies are needed to confirm its effectiveness in clinical practice.
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Zhenchun
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Critical Care Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: An
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, The traditional Chinese medical hospital of Chongqing, Chongqing, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zuo
authors.affiliation: Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D004423:Ear / Q000098:blood supply; D017078:Laser-Doppler Flowmetry; D008833:Microcirculation; D011817:Rabbits; D012771:Shock, Hemorrhagic / Q000175:diagnosis* / Q000503:physiopathology*; D013997:Time Factors