title: Protective effects of caffeic acid phenethyl ester against acute radiation-induced hepatic injury in rats.
abstract: Caffeic acid phenyl ester (CAPE) is a potent anti-inflammatory agent and it can eliminate the free radicals. The current study was intended to evaluate the protective effect of CAPE against the acute radiation-induced liver damage in rats. Male Sprague-Dawley rats were intraperitoneally administered with CAPE (30 mg/kg) for 3 consecutive days before exposing them to a single dose of 30 Gy of β-ray irradiation to upper abdomen. We found that pretreatment with CAPE significantly decreased the serum levels of alanine aminotransferase and aspartate aminotransferase and increased the activity of superoxide dismutase and glutathione. Histological evaluation further confirmed the protection of CAPE against radiation-induced hepatotoxicity. TUNEL assay showed that CAPE pretreatment inhibited hepatocyte apoptosis. Moreover, CAPE inhibited the nuclear transport of NF-κB p65 subunit, decreased the level of tumor necrosis factor-α, nitric oxide and inducible nitric oxide synthase. Taken together, these results suggest that pretreatment with CAPE offers protection against radiation-induced hepatic injury.
authors.affiliation: Department of Oncology, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, 214122 Wuxi, China.
authors.forename: JianJun
authors.initials: J
authors.lastname: Chu
authors.affiliation: Department of Oncology, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, 214122 Wuxi, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, 214122 Wuxi, China.
authors.forename: Liugen
authors.initials: L
authors.lastname: Jin
authors.affiliation: Wuxi Medical School, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Wuxi Medical School, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Wuxi Medical School, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, China. Electronic address: qfpang@jiangnan.edu.cn.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Pang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002109:Caffeic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008107:Liver Diseases / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014965:X-Rays / Q000009:adverse effects
chemical_list: D002109:Caffeic Acids; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol
keywords: Apoptosis; Caffeic acid phenyl ester; Liver; Radiation