title: Cyclic adenosine 5'-diphosphoribose (cADPR) mimics used as molecular probes in cell signaling.
abstract: Cyclic adenosine 5'-diphosphate ribose (cADPR) is a second messenger in the Ca(2+) signaling pathway. To elucidate its molecular mechanism in calcium release, a series of cADPR analogues with modification on ribose, nucleobase, and pyrophosphate have been investigated. Among them, the analogue with the modification of the northern ribose by ether linkage substitution (cIDPRE) exhibits membrane-permeate Ca(2+) agonistic activity in intact HeLa cells, human T cells, mouse cardiac myocytes and neurosecretory PC12 cell lines; thus, cIDPRE and coumarin-caged cIDPRE are valuable probes to investigate the cADPR-mediated Ca(2+) signal pathway.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, P. R. China. liangren@bjmu.edu.cn.
authors.forename: Liangren
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yue
authors.forename: Li-He
authors.initials: LH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D003374:Coumarins / Q000737:chemistry; D036563:Cyclic ADP-Ribose / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007291:Inosine Monophosphate / Q000031:analogs & derivatives / Q000737:chemistry; D019169:Jurkat Cells; D051379:Mice; D018716:Molecular Mimicry; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D003374:Coumarins; D015335:Molecular Probes; C494342:N(1)-((5''-O-phosphorylethoxy)methyl)-5'-O-phosphorylinosine 5',5''-cyclic pyrophosphate; D036563:Cyclic ADP-Ribose; D007291:Inosine Monophosphate; C030123:coumarin; D002118:Calcium
keywords: calcium; cell cycle; nucleotides; signal transduction; structure-activity relationships