title: Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression.
abstract: E-cadherin inactivation underpins the progression of invasive lobular breast carcinoma (ILC). In ILC, p120-catenin (p120) translocates to the cytosol where it controls anchorage independence through the Rho-Rock signaling pathway, a key mechanism driving tumor growth and metastasis. We now demonstrate that anchorage-independent ILC cells show an increase in nuclear p120, which results in relief of transcriptional repression by Kaiso. To identify the Kaiso target genes that control anchorage independence we performed genome-wide mRNA profiling on anoikis-resistant mouse ILC cells, and identified 29 candidate target genes, including the established Kaiso target Wnt11. Our data indicate that anchorage-independent upregulation of Wnt11 in ILC cells is controlled by nuclear p120 through inhibition of Kaiso-mediated transcriptional repression. Finally, we show that Wnt11 promotes activation of RhoA, which causes ILC anoikis resistance. Our findings thereby establish a mechanistic link between E-cadherin loss and subsequent control of Rho-driven anoikis resistance through p120- and Kaiso-dependent expression of Wnt11.
authors.affiliation: Department of Pathology, UMC Utrecht, 3584 CX Utrecht, The Netherlands.
authors.forename: Robert A H
authors.initials: RA
authors.lastname: van de Ven
authors.affiliation: Department of Pathology, UMC Utrecht, 3584 CX Utrecht, The Netherlands.
authors.forename: Milou
authors.initials: M
authors.lastname: Tenhagen
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands. Division of Molecular Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands. Faculty of Electrical Engineering, Mathematics and Computer Science, Delft University of Technology, Mekelweg, 2628 CD Delft, The Netherlands.
authors.forename: Wouter
authors.initials: W
authors.lastname: Meuleman
authors.affiliation: Department of Pathology, UMC Utrecht, 3584 CX Utrecht, The Netherlands.
authors.forename: Jeske J G
authors.initials: JJ
authors.lastname: van Riel
authors.affiliation: Department of Pathology, UMC Utrecht, 3584 CX Utrecht, The Netherlands.
authors.forename: Ron C J
authors.initials: RC
authors.lastname: Schackmann
authors.affiliation: Department of Pathology, UMC Utrecht, 3584 CX Utrecht, The Netherlands. p.w.b.derksen@umcutrecht.nl.
authors.forename: Patrick W B
authors.initials: PW
authors.lastname: Derksen
mesh_terms: D000818:Animals; D023102:Anoikis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D018275:Carcinoma, Lobular / Q000235:genetics / Q000473:pathology*; D051177:Catenins / Q000378:metabolism*; D002448:Cell Adhesion; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000235:genetics / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D021381:Protein Transport; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics; D051153:Wnt Proteins / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism; D000095002:Delta Catenin
chemical_list: D051177:Catenins; D012097:Repressor Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; C508957:Wnt11 protein, mouse; C118822:Zbtb33 protein, mouse; D020742:rhoA GTP-Binding Protein; D000095002:Delta Catenin
keywords: Anoikis resistance; Breast cancer metastasis; Kaiso; p120-catenin