title: Development of bioorthogonal reactions and their applications in bioconjugation.
abstract: Biomolecule labeling using chemical probes with specific biological activities has played important roles for the elucidation of complicated biological processes. Selective bioconjugation strategies are highly-demanded in the construction of various small-molecule probes to explore complex biological systems. Bioorthogonal reactions that undergo fast and selective ligation under bio-compatible conditions have found diverse applications in the development of new bioconjugation strategies. The development of new bioorthogonal reactions in the past decade has been summarized with comments on their potentials as bioconjugation method in the construction of various biological probes for investigating their target biomolecules. For the applications of bioorthogonal reactions in the site-selective biomolecule conjugation, examples have been presented on the bioconjugation of protein, glycan, nucleic acids and lipids.
authors.affiliation: Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. zhengmengmeng453@163.com.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. ndzhengli@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. zpy1994121@163.com.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. jinboli@nju.edu.cn.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. njuzy@nju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D008055:Lipids; D009696:Nucleic Acids; D011506:Proteins