title: Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells.
abstract: Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells, and 24 hours later, cells were transfected with recombinant adenovirus carrying channelrhodopsin-2 and green fluorescent protein genes. After 2-4 days of transfection, green fluorescence was visible in the cultured cells. Cells were stimulated with blue light (470 nm), and light-induced action potentials were recorded in patch-clamp experiments. Our findings indicate that channelrhodopsin-2-recombinant adenovirus transfection of primary cultured visual cortical cells can control the production of action potentials via blue light stimulation.
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Chongqing Key Lab of Visual Damage and Regeneration & Restoration, Chongqing 400038, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Yao
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Chongqing Key Lab of Visual Damage and Regeneration & Restoration, Chongqing 400038, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Ophthalmology, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Chuanhuang
authors.initials: C
authors.lastname: Weng
authors.affiliation: Department of Ophthalmology, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Zhengqin
authors.initials: Z
authors.lastname: Yin
keywords: action potentials; blue light; channelrhodopsin-2; neural regeneration; recombinant adenovirus; visual cortex