title: Herpes simplex virus 1 upregulates p35, alters CDK-5 localization, and stimulates CDK-5 kinase activity during acute infection in neurons.
abstract: The cyclin-dependent kinase 5 (CDK-5) activating protein, p35, is important for acute herpes simplex virus 1 (HSV-1) replication in mice. This report shows that HSV-1 increases p35 levels, changes the primary localization of CDK-5 from the nucleus to the cytoplasm, and enhances CDK-5 activity during lytic or acute infection. Infected neurons also stained positive for the DNA damage response (DDR) marker γH2AX. We propose that CDK-5 is activated by the DDR to protect infected neurons from apoptosis.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.
authors.forename: Heba H
authors.initials: HH
authors.lastname: Mostafa
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: van Loben Sels
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA ddavido@ku.edu.
authors.forename: David J
authors.initials: DJ
authors.lastname: Davido
mesh_terms: D000818:Animals; D017209:Apoptosis; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D004249:DNA Damage; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006657:Histones / Q000032:analysis; D054884:Host-Pathogen Interactions; D018345:Mice, Knockout; D009474:Neurons / Q000821:virology*; D010770:Phosphotransferases / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: C492236:Cdk5r1 protein, mouse; D006657:Histones; C475961:gamma-H2AX protein, mouse; D010770:Phosphotransferases; D051360:Cyclin-Dependent Kinase 5; C495906:Cdk5 protein, mouse