title: Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers.
abstract: The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in Alzheimer's disease (AD). Recent studies have implicated the filamentous actin (F-actin) severing protein, Cofilin, in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However, whether Cofilin is an essential component of the AD pathogenic process and how Aβ impinges its signals to Cofilin from the neuronal surface are unknown. In this study, we found that Aβ42 oligomers (Aβ42O, amyloid-β protein 1-42 oligomers) bind with high affinity to low or intermediate activation conformers of β1-integrin, resulting in the loss of surface β1-integrin and activation of Cofilin via Slingshot homology-1 (SSH1) activation. Specifically, conditional loss of β1-integrin prevented Aβ42O-induced Cofilin activation, and allosteric modulation or activation of β1-integrin significantly reduced Aβ42O binding to neurons while blocking Aβ42O-induced reactive oxygen species (ROS) production, mitochondrial dysfunction, depletion of F-actin/focal Vinculin, and apoptosis. Cofilin, in turn, was required for Aβ42O-induced loss of cell surface β1-integrin, disruption of F-actin/focal Talin-Vinculin, and depletion of F-actin-associated postsynaptic proteins. SSH1 reduction, which mitigated Cofilin activation, prevented Aβ42O-induced mitochondrial Cofilin translocation and apoptosis, while AD brain mitochondria contained significantly increased activated/oxidized Cofilin. In mechanistic support in vivo, AD mouse model (APP (amyloid precursor protein)/PS1) brains contained increased SSH1/Cofilin and decreased SSH1/14-3-3 complexes, indicative of SSH1-Cofilin activation via release of SSH1 from 14-3-3. Finally, genetic reduction in Cofilin rescued APP/Aβ-induced synaptic protein loss and gliosis in vivo as well as deficits in long-term potentiation (LTP) and contextual memory in APP/PS1 mice. These novel findings therefore implicate the essential involvement of the β1-integrin-SSH1-Cofilin pathway in mitochondrial and synaptic dysfunction in AD.
authors.affiliation: 1] Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Woo
authors.affiliation: 1] Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.affiliation: USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: H
authors.initials: H
authors.lastname: Khan
authors.affiliation: USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: C
authors.initials: C
authors.lastname: Penn
authors.affiliation: 1] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] Molecular Pharmacology and Physiology, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] Molecular Pharmacology and Physiology, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: A
authors.initials: A
authors.lastname: Joly-Amado
authors.affiliation: 1] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] Molecular Pharmacology and Physiology, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: E
authors.initials: E
authors.lastname: Weeber
authors.affiliation: 1] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] Molecular Pharmacology and Physiology, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.affiliation: 1] Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL, USA [2] USF Health Byrd Alzheimer's Institute, University of South Florida Morsani College of Medicine, Tampa, FL, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Kang
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D004594:Electrophysiology; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
chemical_list: D051339:Actin Depolymerizing Factors; D016229:Amyloid beta-Peptides; D019012:Integrin beta1; D017382:Reactive Oxygen Species; D010749:Phosphoprotein Phosphatases; C484329:SSH1 protein, human