title: Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.
abstract: MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. Version 20 of the miRNA registry miRBase contains 307 mature miRNAs and 200 precursor sequences for CGR/CHO. We searched for evolutionary conserved miRNAs from miRBase v20 in recently published genomic data, derived from Chinese hamster and CHO cells, to further extend the list of known miRNAs. With our approach we could identify several hundred miRNA sequences in the genome. For several of these, the expression in CHO cells could be verified from multiple next-generation sequencing experiments. In addition, several hundred unexpressed miRNAs are awaiting further confirmation by testing for their transcription in different Chinese hamster tissues.
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria. andreas.diendorfer@boku.ac.at.
authors.forename: Andreas B
authors.initials: AB
authors.lastname: Diendorfer
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hackl
authors.affiliation: ACIB GmbH, Austrian Centre of Industrial Biotechnology, Graz, Austria.
authors.forename: Gerald
authors.initials: G
authors.lastname: Klanert
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Jadhav
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Manuel
authors.initials: M
authors.lastname: Reithofer
authors.affiliation: Biberach University of Applied Sciences, Biberach, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Stiefel
authors.affiliation: Biberach University of Applied Sciences, Biberach, Germany.
authors.forename: Friedemann
authors.initials: F
authors.lastname: Hesse
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.affiliation: Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.|ACIB GmbH, Austrian Centre of Industrial Biotechnology, Graz, Austria.
authors.forename: Nicole
authors.initials: N
authors.lastname: Borth
mesh_terms: D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation
chemical_list: D035683:MicroRNAs
keywords: Chinese hamster ovary cell; microRNA; next-generation sequencing