title: Novel recombinant anti-HER2/neu immunotoxin: design and antitumor efficiency.
abstract: The novel HER2/neu-specific recombinant immunotoxin 4D5scFv-PE40 consisting of 4D5scFv antibody (targeting module) and Pseudomonas exotoxin A fragment (effector module) combined in a single polypeptide chain via a flexible linker has been expressed and purified. This immunotoxin conserves specificity and affinity that are characteristics of the parental antibody 4D5scFv and exhibits selective and strong cytotoxic effect against cancer cells overexpressing HER2/neu receptor. The results of the experiments both in vitro (in cell cultures) and in vivo (in tumor-bearing animals) demonstrate high potential of 4D5scFv-PE40 for targeted therapy of tumors overexpressing HER2/neu.
authors.affiliation: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, 603950, Russia. unn@unn.ru.
authors.forename: E A
authors.initials: EA
authors.lastname: Sokolova
authors.forename: T A
authors.initials: TA
authors.lastname: Zdobnova
authors.forename: O A
authors.initials: OA
authors.lastname: Stremovskiy
authors.forename: I V
authors.initials: IV
authors.lastname: Balalaeva
authors.forename: S M
authors.initials: SM
authors.lastname: Deyev
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005098:Exotoxins / Q000235:genetics; D005260:Female; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D051379:Mice; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D057127:Single-Chain Antibodies / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D000970:Antineoplastic Agents; D001427:Bacterial Toxins; D005098:Exotoxins; D000922:Immunotoxins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A