title: Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme.
abstract: We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
authors.forename: Chaesun
authors.initials: C
authors.lastname: Hong
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Ahruem
authors.initials: A
authors.lastname: Baek
authors.forename: Hyewon
authors.initials: H
authors.lastname: Chung
authors.forename: Woong
authors.initials: W
authors.lastname: Jung
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
mesh_terms: D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D005470:Fluorometry; D006108:Graphite / Q000737:chemistry*; D009154:Mutation; D010087:Oxides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D010087:Oxides; D012313:RNA; D006108:Graphite