title: Preclinical Evaluation of Tegaderm™ Supported Nanofibrous Wound Matrix Dressing on Porcine Wound Healing Model.
abstract: Objective:Nanofibers for tissue scaffolding and wound dressings hold great potential in realizing enhanced healing of wounds in comparison with conventional counterparts. Previously, we demonstrated good fibroblast adherence and growth on a newly developed scaffold, Tegaderm™-Nanofiber (TG-NF), made from poly ɛ-caprolactone (PCL)/gelatin nanofibers electrospun onto Tegaderm (TG). The purpose of this study is to evaluate the performance and safety of TG-NF dressings in partial-thickness wound in a pig healing model.Approach:To evaluate the rate of reepithelialization, control TG, human dermal fibroblast-seeded TG-NF(+) and -unseeded TG-NF(-) were randomly dressed onto 80 partial-thickness burns created on four female and four male pigs. Wound inspections and dressings were done after burns on day 7, 14, 21, and 28. On day 28, full-thickness biopsies were taken for histopathological evaluation by Masson-Trichrome staining for collagen and hematoxylin-eosin staining for cell counting.Results:No infection and severe inflammation were recorded. Wounds treated with TG-NF(+) reepithelialized significantly faster than TG-NF(-) and control. Wound site inflammatory responses to study groups were similar as total cell counts on granulation tissues show no significant differences. Most of the wounds completely reepithelialized by day 28, except for two wounds in control and TG-NF(-). A higher collagen coverage was also recorded in the granulation tissues treated with TG-NF(+).Innovation and Conclusion:With better reepithelialization achieved by TG-NF(+) and similar rates of wound closure by TG-NF(-) and control, and the absence of elevated inflammatory responses to TG-NF constructs, TG-NF constructs are safe and demonstrated good healing potentials that are comparable to Tegaderm.
authors.affiliation: Department of Surgery, National University of Singapore , Singapore, Singapore .
authors.forename: Chee Tian
authors.initials: CT
authors.lastname: Ong
authors.affiliation: Division of Bioengineering, National University of Singapore , Singapore, Singapore . ; Department of Bioengineering, National University of Singapore , Singapore, Singapore .
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Surgery, National University of Singapore , Singapore, Singapore .
authors.forename: Raymond
authors.initials: R
authors.lastname: Lim
authors.affiliation: Division of Bioengineering, National University of Singapore , Singapore, Singapore .
authors.forename: Rebekah
authors.initials: R
authors.lastname: Samsonraj
authors.affiliation: Department of Surgery, National University of Singapore , Singapore, Singapore .
authors.forename: Jeyakumar
authors.initials: J
authors.lastname: Masilamani
authors.affiliation: Division of Bioengineering, National University of Singapore , Singapore, Singapore .
authors.forename: Tran Hong Ha
authors.initials: TH
authors.lastname: Phan
authors.affiliation: Division of Bioengineering, National University of Singapore , Singapore, Singapore .
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Department of Surgery, National University of Singapore , Singapore, Singapore .
authors.forename: Ivor
authors.initials: I
authors.lastname: Lim
authors.affiliation: SingHealth Experimental Medicine Centre, Donghua University , Shanghai, PR China .
authors.forename: Irene
authors.initials: I
authors.lastname: Kee
authors.affiliation: SingHealth Experimental Medicine Centre, Donghua University , Shanghai, PR China .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Fahamy
authors.affiliation: SingHealth Experimental Medicine Centre, Donghua University , Shanghai, PR China .
authors.forename: Vilma
authors.initials: V
authors.lastname: Templonuevo
authors.affiliation: Division of Bioengineering, National University of Singapore , Singapore, Singapore . ; Mechanobiology Institute, National University of Singapore , Singapore, Singapore .
authors.forename: Chwee Teck
authors.initials: CT
authors.lastname: Lim
authors.affiliation: Department of Surgery, National University of Singapore , Singapore, Singapore . ; Faculty of Dentistry Centre for Craniofacial and Regenerative Biology, National University of Singapore , Singapore, Singapore . ; NUS Tissue Engineering and Stem Cell Research Program, National University of Singapore , Singapore, Singapore .
authors.forename: Toan Thang
authors.initials: TT
authors.lastname: Phan