title: A new species of Drepanocephalus Dietz, 1909 (Digenea: Echinostomatidae) from the double-crested cormorant Phalacrocorax auritus (Lesson) (Aves: Phalacrocoracidae) in North America.
abstract: Drepanocephalus auritus n. sp. is described based on specimens from the double-crested cormorant Phalacrocorax auritus (Lesson) in North America. The new species differs from its congeners in its very narrow, elongate body, long uterine field and widely separated testes. Sequences of the nuclear rRNA gene cluster, spanning the 3' end of the nuclear ribosomal 18S rRNA gene, internal transcribed spacer region (ITS1+5.8S gene+ITS2) and partial 28S gene (2,345 bp), were identical in specimens collected from North Dakota, Minnesota and Mississippi, USA. Sequences of the 651 bp long fragment of the mitochondrial cox1 gene exhibited very low intraspecific variability (< 1%). Comparisons of the newly-generated sequences with those available in the GenBank indicate that the sequences from North America published under the name D. spathans Dietz, 1909 in fact represent D. auritus n. sp.
authors.affiliation: Laboratory of Parasitology, Nature Research Centre, Akademijos 2, 08412, Vilnius, Lithuania, olena.kudlai@gmail.com.
authors.forename: Olena
authors.initials: O
authors.lastname: Kudlai
authors.forename: Aneta
authors.initials: A
authors.lastname: Kostadinova
authors.forename: Eric E
authors.initials: EE
authors.lastname: Pulis
authors.forename: Vasyl V
authors.initials: VV
authors.lastname: Tkach
mesh_terms: D000818:Animals; D001717:Birds / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004450:Echinostomatidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D003576:Electron Transport Complex IV / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009656:North America; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV