title: MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3.
abstract: Glioblastoma multiforme (GBM) is the most malignant brain tumor in humans. Previous studies have demonstrated that microRNA plays important roles in the development and proliferation of GBM cells. Here we defined the mechanism by which miR-212-3p regulated the proliferation of GBM. In this study, we showed that miR-212-3p expression was significantly down-regulated and negatively correlated with serum and glucocorticoid-inducible kinase 3 (SGK3) in GBM. Either over-expression of miR-212-3p or silence of SGK3 decreased viability of GBM cells. Moreover, miR-212-3p directly bound to 3'UTR of SGK3 and inhibited its mRNA and protein expression. And over-expression of SGK3 rescued the decreased proliferation of GBM cells induced by miR-212-3p. Importantly, miR-212-3p also suppressed tumor growth in vivo. Collectively, our results demonstrated that miR-212-3p inhibited proliferation of GBM cells by directly targeting SGK3, and could potentially serve as a new therapeutic target for GBM.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No.23 Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang Province, People's Republic of China.
authors.forename: Huailei
authors.initials: H
authors.lastname: Liu
authors.forename: Chenguang
authors.initials: C
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Shen
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
authors.forename: Kaikai
authors.initials: K
authors.lastname: Wang
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Liu
authors.forename: Bingjie
authors.initials: B
authors.lastname: Zheng
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Jianing
authors.initials: J
authors.lastname: Wu
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhong
authors.forename: Jiakang
authors.initials: J
authors.lastname: Zhang
authors.forename: Huaizhang
authors.initials: H
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005909:Glioblastoma / Q000523:psychology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016014:Linear Models; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D023041:Xenograft Model Antitumor Assays; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D049452:Green Fluorescent Proteins; D035683:MicroRNAs; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C551411:MIRN212 microRNA, human; C403243:SGK3 protein, human; D000099184:Serum-Glucocorticoid Regulated Kinases