title: The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency.
abstract: The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Maheswara Reddy
authors.initials: MR
authors.lastname: Emani
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Elisa
authors.initials: E
authors.lastname: Närvä
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Aki
authors.initials: A
authors.lastname: Stubb
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Deepankar
authors.initials: D
authors.lastname: Chakroborty
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Miro
authors.initials: M
authors.lastname: Viitala
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Rokka
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Nelly
authors.initials: N
authors.lastname: Rahkonen
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Robert
authors.initials: R
authors.lastname: Moulder
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Denessiouk
authors.affiliation: Research Programs Unit, Molecular Neurology and Biomedicum Stem Cell Center, University of Helsinki, 00290 Helsinki, Finland.
authors.forename: Ras
authors.initials: R
authors.lastname: Trokovic
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Riikka
authors.initials: R
authors.lastname: Lund
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland; Department of Mathematics and Statistics, University of Turku, 20014 Turku, Finland.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Elo
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland. Electronic address: riitta.lahesmaa@btk.fi.
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D000066673:Cell Self Renewal / Q000187:drug effects / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D061988:Proteasome Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C562346:L1TD1 protein, human; D061988:Proteasome Inhibitors; D011506:Proteins; D016601:RNA-Binding Proteins