title: CUGBP1 promotes cell proliferation and suppresses apoptosis via down-regulating C/EBPα in human non-small cell lung cancers.
abstract: CUGBP1, which is involved in posttranscriptional regulatory networks, may control cell growth, activation and differentiation. Meanwhile, CCAAT/enhancer-binding protein α (C/EBPα) acts as a basic leucine zipper transcription factor which controls differentiation-dependent gene expression and inhibits cell proliferation. To date, very little is known about the association between CUGBP 1 and C/EBPα in regulating cell proliferation and apoptosis in non-small cell lung cancer (NSCLC). CUGBP1 and C/EBPα mRNA expressions were analyzed in NSCLC tumor and adjacent normal tissues, and the relationship in clinicopathological parameters was evaluated. Knockdown of CUGBP1 and C/EBPα regulated by CUGBP1 in NSCLC cell line was identified by real-time PCR and Western blot. The effect of depletion of CUGBP1 was evaluated by MTT assay and Annexin/Propidium Iodide Apoptosis assay. CUGBP1 is highly expressed and expression of C/EBPα is low in NSCLC tissues. The correlation analysis revealed that there was negative correlation between the expression of CUGBP 1 and C/EBPα. Knockdown of CUGBP1 effectively silenced the expression of CUGBP1 and up-regulated C/EBPα. Also, suppression of CUGBP1 inhibits proliferation and induces apoptosis in A549 cells. These observations suggest that the first proof the overexpression of CUGBP1 in NSCLC contributes to tumorigenesis through down-regulation of C/EBPα. Knockdown of CUGBP1 or up-regulation C/EBPα might be a potential therapeutic approach for human non-small cell lung cancers.
authors.affiliation: Department of Oncology, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, Shandong, People's Republic of China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Lu
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Yu
authors.forename: Shihai
authors.initials: S
authors.lastname: Liu
authors.forename: Lianhua
authors.initials: L
authors.lastname: Cui
authors.forename: Xiaozheng
authors.initials: X
authors.lastname: Chen
authors.forename: Ruyong
authors.initials: R
authors.lastname: Yao
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism*; D000067879:CELF1 Protein / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012016:Reference Values
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D000067879:CELF1 Protein; C102843:CELF1 protein, human