title: Influence of succinylation on the conformation of yak casein micelles.
abstract: Succinylation modifies the physicochemical characteristics and improves the functional properties of proteins. This study assessed the effects of succinylation on the conformation of yak casein micelles with seven degree of modification. The results revealed that succinylation contributed to the dissociation of casein micelles. With the increase of succinylated degree, soluble nitrogen and minerals content increased, while casein micelle size and polydispersity index of micelles decreased. Succinylation affected the spatial conformation of yak casein micelles: turn decreased, ß-sheet and α-helix increased, and irregular structure were non-significantly affected. The intrinsic and ANS fluorescence intensity decreased and the maximum emission wavelength shifted red with increasing succinylation. Based on the results, the structure of yak casein micelles was characteristic of the sub-micelle model.
authors.affiliation: College of Science, Gansu Agricultural University, Lanzhou, China; College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Functional Dairy Product Engineering Lab of Gansu Province, Lanzhou, China. Electronic address: yangmin@gsau.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Functional Dairy Product Engineering Lab of Gansu Province, Lanzhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Cui
authors.affiliation: Instrumental Research and Analysis Center of Gansu Agricultural University, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Functional Dairy Product Engineering Lab of Gansu Province, Lanzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Science, Gansu Agricultural University, Lanzhou, China.
authors.forename: Jitao
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Science, Gansu Agricultural University, Lanzhou, China.
authors.forename: Jiangyu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D002364:Caseins; D008823:Micelles; D019802:Succinic Acid
keywords: Casein (CID: 73995022); Conformation; DLS; FTIR; Fluorescence spectroscopy; Succinic anhydride (CID: 7922); Succinylation; Yak casein micelles