title: 3B, a novel of photosensitizer, exhibited anti-tumor effects via mitochondrial apoptosis pathway in MCF-7 human breast carcinoma cells.
abstract: Photodynamic therapy (PDT) has been recognized as an innovated therapeutic modality for the treatment of various cancers. In this study, we evaluated the anticancer effect of a new photosensitizer 3B in breast cancer, which was considered one of the most common cancers in women worldwide. Here, we determined the effect of 3B not only on the cell growth, apoptosis, and Bcl-2 signal pathway in vitro but also on the anti-cancer effect in nude mice in vivo. Our results showed that 3B was primarily accumulated in mitochondria, increased the level of ROS, induced apoptotic cells death via Bcl-2 family, and its activity could be blocked by the caspase inhibitor (Z-VAD-FMK). In vivo study, 3B made a significant opening inhibition of tumor growth and showed drug toxicity hardly. TUNEL assay indicated that PDT group showed more positive cells (green) than other groups. These data supported that 3B might develop as potential therapeutic drug for the treatment of breast cancer.
authors.affiliation: Department of Molecular & Cellular Pharmacology, Biomedical Nanotechnology Center, State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Lei
authors.forename: Shaoying
authors.initials: S
authors.lastname: Tan
authors.forename: Wenpei
authors.initials: W
authors.lastname: Du
authors.forename: Yichun
authors.initials: Y
authors.lastname: Xu
authors.forename: Shengchao
authors.initials: S
authors.lastname: Lin
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zou
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D007094:Imides / Q000008:administration & dosage*; D061986:MCF-7 Cells; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D043803:Phenalenes / Q000008:administration & dosage*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D007094:Imides; D043803:Phenalenes; D017319:Photosensitizing Agents; D019253:Proto-Oncogene Proteins c-bcl-2