title: NMR assignments for the insertion domain of bacteriophage CUS-3 coat protein.
abstract: CUS-3 is a P22-like tailed dsDNA bacteriophage that infects Escherichia coli serotype K1. The CUS-3 coat protein, which forms the icosahedral capsid, has a conserved HK97-fold but with a non-conserved accessory domain known as the insertion domain (I-domain). Sequence alignment of the coat proteins from CUS-3 and P22 shows higher sequence similarity for the I-domains (35 %) than for the HK97-cores, suggesting the I-domains play important functional roles. The I-domain of the P22 coat protein, which has an NMR structure comprised of a six-stranded β-barrel, has been shown to govern the assembly, stability and size of the resulting capsid particles. Here, we report the (1)H, (15)N, and (13)C assignments for the I-domain from the coat protein of bacteriophage CUS-3. The secondary structure and dynamics of the CUS-3 I-domain, predicted from the assigned NMR chemical shifts, agree with those of the P22 I-domain, suggesting the CUS-3 and P22 I-domains may have similar structures and functions in capsid assembly.
authors.affiliation: Department of Molecular & Cell Biology, and Chemistry, University of Connecticut, 91 N. Eagleville Road., Storrs, CT, 06269-3125, USA.
authors.forename: Therese N
authors.initials: TN
authors.lastname: Tripler
authors.affiliation: Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health, 263 Farmington Ave., Farmington, CT, 06030-3305, USA.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Maciejewski
authors.affiliation: Department of Molecular & Cell Biology, and Chemistry, University of Connecticut, 91 N. Eagleville Road., Storrs, CT, 06269-3125, USA. teschke@uconn.edu.|Department of Chemistry, University of Connecticut, 55 N. Eagleville Rd., Storrs, CT, 06269-3060, USA. teschke@uconn.edu.
authors.forename: Carolyn M
authors.initials: CM
authors.lastname: Teschke
authors.affiliation: Department of Molecular & Cell Biology, and Chemistry, University of Connecticut, 91 N. Eagleville Road., Storrs, CT, 06269-3125, USA. andrei@uconn.edu.
authors.forename: Andrei T
authors.initials: AT
authors.lastname: Alexandrescu
mesh_terms: D000595:Amino Acid Sequence; D017100:Bacteriophage P22 / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D036022:Capsid Proteins
keywords: Bacteriophage; CUS-3; I-domain; Procapsid; Viral assembly