title: Isolation and purification of schisandrol A from the stems of Schisandra chinensis and cytotoxicity against human hepatocarcinoma cell lines.
abstract: BACKGROUND
  Schisandrol A, a lignan with anticancer effects, is one of the representative components that identifies Schisandra chinensis.
  
  
  OBJECTIVE
  A method for purifying schisandrol A from the stems of S. chinensis was established using an octadecylsilyl (ODS) column combined with preparative high-performance liquid chromatography (HPLC).
  
  
  METHODS
  Crude extracts obtained from the stems of S. chinensis using 70% ethanol were separated on an AB-8 macroporous resin column and then eluted with a graded ethanol series. After 70% methanol was used in an ODS column separation, preparative HPLC was used for subsequent purification. The structure was identified by electrospray ionization-mass spectrometry, (1)H-nuclear magnetic resonance, and (13)C-nuclear magnetic resonance. HepG2 and Bel-7402 hepatocellular carcinoma cell lines were used for toxicological evaluation.
  
  
  RESULTS
  21.4 mg of schisandrol A with a purity of 95.2% were collected. The cytotoxicity of the ODS-purified sample and schisandrol A were significantly stronger than that of a resin-purified sample.
  
  
  CONCLUSIONS
  Schisandrol A was successfully extracted from the stems of S. chinensis and separated with an ODS column combined with preparative HPLC. The samples obtained during the purification process showed different levels of cytotoxicity on the HepG2 and Bel-7402 hepatocellular carcinoma cell lines.
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Horticulture, Eastern Liaoning University, Dandong 118003, People's Republic of China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Huang
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Meng
keywords: Cytotoxicity; Schisandra chinensis; octadecylsilyl; preparative high-performance liquid chromatography; schisandrol A