title: Chronic monitoring of vascular progression after ischemic stroke using multiexposure speckle imaging and two-photon fluorescence microscopy.
abstract: Monitoring the progression of the vascular structure and cerebral blood flow (CBF) after brain injury is vital to understand the neurovascular recovery process. Multiexposure speckle imaging (MESI) provides a quantitatively accurate technique for chronically measuring the postocclusion CBF perfusion of the infarct and peri-infarct regions in rodent stroke models, while multiphoton microscopy offers direct visualization of the microvascular structure. In this paper, we present imaging outcomes extending 35 days after photo-thrombotic occlusion, tracking the progression of the vasculature throughout this period. We compare MESI flow estimates within the unresolvable parenchyma with subsurface microvascular volume fractions taken with two-photon microscopy in the same regions to assess how the vascular density influences the surface-integrated MESI flow values. The MESI flow measurements and volume fractions are shown to have high correlations (r=0.90) within areas of recovering vasculature in the peri-infarct region. We also observe vascular reorientation occurring within the microvascular structure throughout the 35-day postocclusion period. With the combination of a chronic mouse model and relatively noninvasive optical imaging techniques, we present an imaging protocol for monitoring long-term vascular progression after photo-thrombotic occlusion with the potential to test the efficacy of rehabilitation and pharmacological therapies.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Schrandt
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: S M Shams
authors.initials: SM
authors.lastname: Kazmi
authors.affiliation: 1] Department of Psychology, The University of Texas at Austin, Austin, Texas, USA [2] Institute for Neuroscience, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Jones
authors.affiliation: 1] Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA [2] Institute for Neuroscience, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Dunn
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000473:pathology; D002560:Cerebrovascular Circulation; D008297:Male; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D055806:Microvessels / Q000473:pathology*; D061848:Optical Imaging; D020521:Stroke / Q000473:pathology*