title: A label-free electrochemical DNA biosensor based on covalent immobilization of salmonella DNA sequences on the nanoporous glassy carbon electrode.
abstract: Herein, an easy and cost-effective approach to the immobilization of probe was performed. The amino modified salmonella ssDNA probe sequence was covalently linked with carboxylic group on the surface of nanoporous glassy carbon electrode to prepare the DNA biosensor. The differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for the determination of salmonella DNA in the concentration ranges of 10-400pM and 1-400pM with limits of detection of 2.1pM and 0.15pM, respectively.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Amouzadeh Tabrizi
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis; D005898:Glass / Q000737:chemistry; D008839:Microelectrodes; D008969:Molecular Sequence Data; D058608:Nanopores / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D004269:DNA, Bacterial; D002244:Carbon
keywords: Covalent immobilization; DNA biosensor; Label-free detection; Nanoporous glassy carbon electrode; Salmonella