title: DYn-2 Based Identification of Arabidopsis Sulfenomes.
abstract: Identifying the sulfenylation state of stressed cells is emerging as a strategic approach for the detection of key reactive oxygen species signaling proteins. Here, we optimized an in vivo trapping method for cysteine sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells using a dimedone based DYn-2 probe. We demonstrated that DYn-2 specifically detects sulfenylation events in an H2O2 dose- and time-dependent way. With mass spectrometry, we identified 226 sulfenylated proteins after H2O2 treatment of Arabidopsis cells, residing in the cytoplasm (123); plastid (68); mitochondria (14); nucleus (10); endoplasmic reticulum, Golgi and plasma membrane (7) and peroxisomes (4). Of these, 123 sulfenylated proteins have never been reported before to undergo cysteine oxidative post-translational modifications in plants. All in all, with this DYn-2 approach, we have identified new sulfenylated proteins, and gave a first glance on the locations of the sulfenomes of Arabidopsis thaliana.
authors.affiliation: From the Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium; Faculty of Biological Sciences, University of Dhaka, 1000 Dhaka, Bangladesh.
authors.forename: Salma
authors.initials: S
authors.lastname: Akter
authors.affiliation: Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium;
authors.forename: Jingjing
authors.initials: J
authors.lastname: Huang
authors.affiliation: From the Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium;
authors.forename: Nandita
authors.initials: N
authors.lastname: Bodra
authors.affiliation: From the Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium;
authors.forename: Barbara
authors.initials: B
authors.lastname: De Smet
authors.affiliation: Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium;
authors.forename: Khadija
authors.initials: K
authors.lastname: Wahni
authors.affiliation: From the Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
authors.forename: Debbie
authors.initials: D
authors.lastname: Rombaut
authors.affiliation: Department of Medical Protein Research, VIB, 9000 Ghent, Belgium; Department of Biochemistry, Ghent University, 9000 Ghent, Belgium;
authors.forename: Jarne
authors.initials: J
authors.lastname: Pauwels
authors.affiliation: Department of Medical Protein Research, VIB, 9000 Ghent, Belgium; Department of Biochemistry, Ghent University, 9000 Ghent, Belgium;
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.affiliation: Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458, USA;
authors.forename: Kate
authors.initials: K
authors.lastname: Carroll
authors.affiliation: From the Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; frank.vanbreusegem@psb.vib-ugent.be joris.messens@vib-vub.be.
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
authors.affiliation: Structural Biology Research Center, VIB, 1050 Brussels, Belgium; Brussels Center for Redox Biology, 1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium; frank.vanbreusegem@psb.vib-ugent.be joris.messens@vib-vub.be.
authors.forename: Joris
authors.initials: J
authors.lastname: Messens
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002451:Cell Compartmentation; D018929:Cell Culture Techniques; D003512:Cyclohexanones / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D015335:Molecular Probes / Q000737:chemistry*; D058977:Molecular Sequence Annotation; D015388:Organelles / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D013434:Sulfenic Acids / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003512:Cyclohexanones; D015335:Molecular Probes; D013434:Sulfenic Acids; D001360:Avidin; D001710:Biotin; C004774:dimedone; D006861:Hydrogen Peroxide