title: IL-33 Enhances Humoral Immunity Against Chronic HBV Infection Through Activating CD4(+)CXCR5(+) TFH Cells.
abstract: This study aimed to investigate the potential effect of interleukin 33 (IL-33) on humoral responses to hepatitis B virus (HBV) and the possible mechanisms underlying the action of IL-33 in regulating follicular helper T (TFH) cells. The impact of IL-33 treatment on the levels of serum HBV DNA, HBsAg, HBeAg, HBsAb, and HBeAb, as well as the frequencies of CD4(+)CXCR5(+) TFH cells in wild-type HBV transgenic (HBV-Tg) mice and in a transwell coculture of HepG2.2.15 with IL-33-treated peripheral blood mononuclear cells (PBMCs) were determined. Furthermore, the gene transcription profiles in IL-33-treated TFH cells were determined by microarrays. IL-33 treatment significantly reduced the levels of serum HBV DNA, HBsAg, and HBeAg, but increased the levels of HBsAb and HBeAb in HBV-Tg mice, accompanied by increased frequency of splenic infiltrating CD4(+)CXCR5(+) TFH cells in HBV-Tg. Similarly, coculture of HepG2.2.15 cells with IL-33-treated PBMCs reduced the levels of HBV DNA, HBsAg, and HBeAg, but increased the levels of HBsAb and HBeAb. Microarray analyses indicated that IL-33 significantly modulated the transcription of many genes involved in regulating TFH activation and differentiation. Our findings suggest that IL-33 may activate TFH cells, promoting humoral responses to HBV during the pathogenic process.
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .
authors.forename: Ping-Wei
authors.initials: PW
authors.lastname: Zhao
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .
authors.forename: Xu
authors.initials: X
authors.lastname: Shi
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: 2 Department of Medical Laboratory Sciences, Haramaya University , Dire Dawa, Ethiopia .
authors.forename: Desalegn Admassu
authors.initials: DA
authors.lastname: Ayana
authors.affiliation: 3 Department of Hepatology, The First Hospital of Jilin University , Changchun, China .
authors.forename: Jun-Qi
authors.initials: JQ
authors.lastname: Niu
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Feng
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun, China .|4 Department of Pediatrics, First Affiliated Hospital of Jiamusi University , Jiamusi, China .
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D018920:Coculture Techniques; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000276:immunology* / Q000472:pathogenicity; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects*; D000067596:Interleukin-33 / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000821:virology; D051379:Mice; D008822:Mice, Transgenic; D054380:Receptors, CXCR5 / Q000819:agonists / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D015704:CD4 Antigens; C095085:CXCR5 protein, human; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; C506536:IL33 protein, human; D000067596:Interleukin-33; D054380:Receptors, CXCR5