title: Complete mitochondrial genome of "Xenopus tropicalis" Asashima line (Anura: Pipidae), a possible undescribed species.
abstract: The diploid Xenopus tropicalis, with its small nuclear genomic size and short generation time compared to the traditional experimental amphibian X. laevis, is considered a next-generation model animal. Several experimental X. tropicalis lines have been used in research studies. Previous studies showed that the mtDNA sequence of the Asashima line is divergent from other lines and that this line may represent a distinct species. Here, we report the complete nucleotide sequence of this unique X. tropicalis experimental line. The genome is 17,700 bp in length and contains 37 genes commonly found in animal mtDNAs. The 16S rRNA gene sequence in Asashima line differed by over 6% from the standard Nigerian lines (a 3% difference is considered the species threshold in anurans), suggesting that this experimental line is a distinct species from the true X. tropicalis.
authors.affiliation: a Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Ibaraki , Japan and.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Haramoto
authors.affiliation: a Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Ibaraki , Japan and.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Oshima
authors.affiliation: b Institute for Amphibian Biology, Graduate school of Science, Hiroshima University , Kagamiyama, Higashi-Hiroshima , Hiroshima , Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: a Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Ibaraki , Japan and.
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
authors.affiliation: a Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Ibaraki , Japan and.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ito
authors.affiliation: b Institute for Amphibian Biology, Graduate school of Science, Hiroshima University , Kagamiyama, Higashi-Hiroshima , Hiroshima , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurabayashi
mesh_terms: D000818:Animals; D001482:Base Composition; D003062:Codon; D023061:Gene Order; D015321:Gene Rearrangement; D050259:Genes, Mitochondrial; D059646:Genome Size; D054629:Genome, Mitochondrial; D016366:Open Reading Frames; D010884:Pipidae / Q000145:classification* / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing
chemical_list: D003062:Codon
keywords: Asashima line; Xenopus tropicalis; experimental animal; mitochondrial genome