title: Guidelines for assessing mouse endothelial function via ultrasound imaging: a report from the International Society Of Cardiovascular Translational Research.
abstract: The study is to establish a novel method to determine the endothelial function in mouse carotid arteries in vivo by using high-resolution ultrasound images. Atherosclerosis in carotid arteries is induced in ApoE(-/-) mice with a Western diet. The ultrasound of the ventral neck generates clear pictures of the common carotid arteries. Acetylcholine at the range from 5 to 20 μg/kg/min (iv) is able to induce a dose-dependent relaxation as shown by the increased diameter of these normal mouse carotid arteries, which is impaired in atherosclerotic arteries. The endothelial function determined by ultrasound images in vivo matches well with that determined in isolated carotid arterial rings in vitro. All animals survived after the endothelial function measurement. In this study, we have established a standard method to determine the mouse endothelial function in vivo. It is a reliable, safe, and survival method that could be used repetitively in mouse arteries.
authors.affiliation: Department of Pharmacology and Cardiovascular Research Center, Rush Medical College, Rush University Medical Center, 1735 West Harrison St, Cohn Building, Suite 406, Chicago, IL, 60612, USA.
authors.forename: Hua-ting
authors.initials: HT
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shan
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Maoping
authors.initials: M
authors.lastname: Chu
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Dan
authors.initials: D
authors.lastname: Yi
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhan
authors.forename: Zu-Yi
authors.initials: ZY
authors.lastname: Yuan
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Raikwar
authors.forename: Shenming
authors.initials: S
authors.lastname: Wang
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging* / Q000503:physiopathology; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000503:physiopathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D018345:Mice, Knockout; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014463:Ultrasonography; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D001057:Apolipoproteins E; D014665:Vasodilator Agents; D000109:Acetylcholine