title: Effect of dietary lead on intestinal nutrient transporters mRNA expression in broiler chickens.
abstract: Lead- (Pb-) induced oxidative stress is known to suppress growth performance and feed efficiency in broiler chickens. In an attempt to describe the specific underlying mechanisms of such phenomenon we carried out the current study. Ninety-six one-day-old broiler chicks were randomly assigned to 2 dietary treatment groups of 6 pen replicates, namely, (i) basal diet containing no lead supplement (control) and (ii) basal diet containing 200 mg lead acetate/kg of diet. Following 3 weeks of experimental period, jejunum samples were collected to examine the changes in gene expression of several nutrient transporters, antioxidant enzymes, and heat shock protein 70 (Hsp70) using quantitative real-time PCR. The results showed that addition of lead significantly decreased feed intake, body weight gain, and feed efficiency. Moreover, with the exception of GLUT5, the expression of all sugar, peptide, and amino acid transporters was significantly downregulated in the birds under Pb induced oxidative stress. Exposure to Pb also upregulated the antioxidant enzymes gene expression together with the downregulation of glutathione S-transferase and Hsp70. In conclusion, it appears that Pb-induced oxidative stress adversely suppresses feed efficiency and growth performance in chicken and the possible underlying mechanism for such phenomenon is downregulation of major nutrient transporter genes in small intestine.
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Roohollah
authors.initials: R
authors.lastname: Ebrahimi
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faseleh Jahromi
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Abdoreza
authors.initials: A
authors.lastname: Soleimani Farjam
authors.affiliation: Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Parisa
authors.initials: P
authors.lastname: Shokryazdan
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Zulkifli
authors.initials: Z
authors.lastname: Idrus
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004032:Diet / Q000379:methods; D019587:Dietary Supplements; D005502:Food; D005786:Gene Expression Regulation / Q000235:genetics*; D005982:Glutathione Transferase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D015430:Weight Gain / Q000235:genetics
chemical_list: D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D007854:Lead; D005982:Glutathione Transferase