title: Investigation of a swimming pool-associated cryptosporidiosis outbreak in the Kimberley region of Western Australia.
abstract: Cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite Cryptosporidium, which manifests primarily as watery diarrhoea. Transmitted via the faecal-oral route, infection with the parasite can occur through ingestion of water, food or other fomites contaminated with its infective oocyst stage. In the months of November and December 2012, there were 18 notified cases of cryptosporidiosis from Broome, Western Australia. The 5-year average for the Kimberley region for this period is <1 case. Interviews conducted by Broome local government staff on the notified cases revealed that 11/18 cases had been swimming at the Broome public swimming pool. Molecular analyses of extracted DNA performed on 8/18 microscopy-positive faecal samples from interviewed cases and three water samples from different locations at the hypervariable glycoprotein 60 (gp60) gene, identified the C. hominis IbA10G2 subtype in all human samples and one water sample.
authors.affiliation: School of Veterinary and Life Sciences,Murdoch University,South Street, Murdoch, WA,Australia.
authors.forename: J S Y
authors.initials: JS
authors.lastname: Ng-Hublin
authors.affiliation: Environmental Health Services,Shire of Broome, Broome, Western Australia,Australia.
authors.forename: D
authors.initials: D
authors.lastname: Hargrave
authors.affiliation: OzFoodNet Communicable Disease Control Directorate,Perth, Western Australia,Australia.
authors.forename: B
authors.initials: B
authors.lastname: Combs
authors.affiliation: School of Veterinary and Life Sciences,Murdoch University,South Street, Murdoch, WA,Australia.
authors.forename: U
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000453:epidemiology*; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis*; D004196:Disease Outbreaks; D005243:Feces / Q000469:parasitology*; D005759:Gastroenteritis / Q000453:epidemiology* / Q000469:parasitology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D013551:Swimming Pools; D014867:Water / Q000469:parasitology; D014904:Western Australia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D014867:Water
keywords: swimming pool