title: Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator superfamily efflux pump with multiple roles.
abstract: Mycobacterium smegmatis mc(2)155 MSMEG_3705 gene was annotated to encode a transporter protein that contains 12 alpha-helical transmembrane domains. We predicted MSMEG_3705 encoding a major facilitator superfamily (MFS) member. To confirm the prediction, the M. smegmatis mc(2)155 MSMEG_3705 gene was deleted. The MSMEG_3705 deletion mutant strain M. smegmatis mc(2)155 ∆MSMEG_3705 was more sensitive to capreomycin. Moreover, M. smegmatis mc(2)155 ∆MSMEG_3705 strain accumulated more ethidium bromide intracellular than wild-type M. smegmatis mc(2)155. Quite unexpectedly, M. smegmatis mc(2)155 ∆MSMEG_3705 grew faster than the wild-type M. smegmatis mc(2)155. The upregulation of the expression of MSMEG_3706, a gene encoding isocitrate lyase downstream MSMEG_3705, in the deletion mutant, might underlie such faster growth in the mutant. The study showed that MSMEG_3705 encodes a genuine MFS member and plays significant role in bacterial growth and antibiotics resistance.
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Three Gorges Eco-environment and Bioresources, Key Laboratory Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, China, 1911125780@qq.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D000995:Antitubercular Agents / Q000378:metabolism; D001693:Biological Transport, Active; D002207:Capreomycin / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D004996:Ethidium / Q000378:metabolism; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000995:Antitubercular Agents; D026901:Membrane Transport Proteins; D002207:Capreomycin; D004996:Ethidium