title: miR-28-5p Involved in LXR-ABCA1 Pathway is Increased in the Plasma of Unstable Angina Patients.
abstract: BACKGROUND
  Previous studies confirmed that the intronic miRNAs participated in regulating host gene-primed biological processes. The coordinated roles of miR-28 with its host gene, LIM domain lipoma-preferred partner (LPP), remain unknown in atherosclerosis.
  
  
  METHODS
  In this study, we determined to assess circulating levels of miR-28-5p in unstable angina patients, compared with age- and sex- matched control subjects by quantitative PCR. Furthermore, we attempted to explore whether miR-28-5p could influence the expression of ATP-binding cassette transporter A1 (ABCA1) and liver X receptor (LXR), major mediators of high density lipoprotein (HDL) synthesis and transportation in hepatic cells and macrophages.
  
  
  RESULTS
  It was found that plasma levels of miR-28-5p were significantly increased in unstable angina patients with or without type 2 diabetes mellitus. Notably, miR-28-5p upregulated ABCA1 expression at transcription and translation levels, strongly correlated with translational activation of LXRα in HepG2 and THP-1-derived macrophages.
  
  
  CONCLUSIONS
  Our findings suggest that circulating miR-28-5p, involved in LXRα-ABCA1 pathway, may be a potential biomarker for diagnosis and prognosis of unstable angina.
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory Center, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiovasology, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: Department of Cardiovasology, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Tangshan Key Laboratory of Clinical Molecular Diagnosis and Treatment, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Tangshan Key Laboratory of Clinical Molecular Diagnosis and Treatment, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Li
authors.affiliation: Tangshan Key Laboratory of Clinical Molecular Diagnosis and Treatment, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China; Department of Clinical Laboratory Center, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: Tangshan Key Laboratory of Clinical Molecular Diagnosis and Treatment, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China; Department of Clinical Laboratory Center, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China. Electronic address: yuntaozhou@hotmail.com.
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Zhou
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China; Department of Cardiovasology, Tangshan Gongren Hospital affiliated to Hebei Medical University, Tangshan, Hebei 063000, P.R. China. Electronic address: Xmsh_ts@163.com.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Shang
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000097:blood*; D000368:Aged; D000789:Angina, Unstable / Q000097:blood* / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D000071518:Liver X Receptors; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D057093:Orphan Nuclear Receptors / Q000097:blood*; D011379:Prognosis; D015398:Signal Transduction
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D015415:Biomarkers; D000071518:Liver X Receptors; C570628:MIRN28 microRNA, human; D035683:MicroRNAs; C000605213:NR1H3 protein, human; D057093:Orphan Nuclear Receptors
keywords: ABCA1; Circulation; LXRα; Unstable angina; miR-28-5p