title: GABAergic projections from lateral hypothalamus to paraventricular hypothalamic nucleus promote feeding.
abstract: Lesions of the lateral hypothalamus (LH) cause hypophagia. However, activation of glutamatergic neurons in LH inhibits feeding. These results suggest a potential importance for other LH neurons in stimulating feeding. Our current study in mice showed that disruption of GABA release from adult LH GABAergic neurons reduced feeding. LH GABAergic neurons project extensively to the paraventricular hypothalamic nucleus (PVH), and optogenetic stimulation of GABAergic LH → PVH fibers induced monosynaptic IPSCs in PVH neurons, and potently increased feeding, which depended on GABA release. In addition, disruption of GABA-A receptors in the PVH reduced feeding. Thus, we have identified a new feeding pathway in which GABAergic projections from the LH to the PVH promote feeding.
authors.affiliation: Brown Foundation Institute of Molecular Medicine and.
authors.forename: Zhaofei
authors.identifier: http://orcid.org/0000-0002-2463-242X
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Brown Foundation Institute of Molecular Medicine and.
authors.forename: Eun Ran
authors.initials: ER
authors.lastname: Kim
authors.affiliation: Brown Foundation Institute of Molecular Medicine and.
authors.forename: Hao
authors.identifier: http://orcid.org/0000-0001-9388-9176
authors.initials: H
authors.lastname: Sun
authors.affiliation: Brown Foundation Institute of Molecular Medicine and.
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Brown Foundation Institute of Molecular Medicine and Graduate Program in Neuroscience of Graduate School of Biological Sciences, University of Texas Medical School at Houston, Houston, Texas 77030.
authors.forename: Leandra R
authors.initials: LR
authors.lastname: Mangieri
authors.affiliation: Division of Anesthesiology and Critical Care, University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030.
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Li
authors.affiliation: Division of Anesthesiology and Critical Care, University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Pan
authors.affiliation: Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, and.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neuroscience and Jan Duncan Neurological Research Institute, Baylor College of Medicine, Houston, Texas, 77030.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Arenkiel
authors.affiliation: Brown Foundation Institute of Molecular Medicine and Graduate Program in Neuroscience of Graduate School of Biological Sciences, University of Texas Medical School at Houston, Houston, Texas 77030, qingchun.tong@uth.tmc.edu.
authors.forename: Qingchun
authors.identifier: http://orcid.org/0000-0002-4561-2540
authors.initials: Q
authors.lastname: Tong
mesh_terms: D000818:Animals; D004435:Eating / Q000502:physiology*; D059330:GABAergic Neurons / Q000378:metabolism / Q000502:physiology*; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000502:physiology*; D053444:Inhibitory Postsynaptic Potentials; D051379:Mice; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000502:physiology*
keywords: GABA; LH; PVH; feeding; optogenetics