title: Overexpression of Hiwi Inhibits the Growth and Migration of Chronic Myeloid Leukemia Cells.
abstract: Chronic myeloid leukemia (CML) is a hematopoietic malignancy characterized by dysregulated growth and proliferation of hematopoietic stem/progenitor cells in bone marrow and excessive expansion of hematopoietic compartments in peripheral blood. Expression deletion of Hiwi, a human Piwi homolog, has been reported to be implicated in leukemogenesis. We here explored Hiwi's role in CML pathogenesis by determining how and whether its forced overexpression could affect CML cell growth and migration. The present results showed that lentivirus-mediated overexpression of Hiwi significantly suppressed cell proliferation and induced obvious apoptosis in K562 cells, a CML line cell line. Tumors in BALB/c nude mice generated by the K562 cells expressing Hiwi were much smaller than those formed by the control cells. Like in vitro, Hiwi upregulation induced cell apoptosis in the tumor tissues in vivo. Additionally, Hiwi elevation suppressed K562 cell migration and inhibited the activity and expression of matrix metalloproteinase-2 and -9. In summary, our study demonstrates that Hiwi overexpression inhibits CML cell growth and migration, providing insights into its role in CML pathogenesis.
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Rheumatology and Immunology, The First Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Bailu
authors.initials: B
authors.lastname: Sang
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cong
authors.affiliation: Cancer Center, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun, Jilin, 130021, People's Republic of China. liuzilinglzl@126.com.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism* / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
chemical_list: D060565:Argonaute Proteins; C421088:PIWIL1 protein, human; D020782:Matrix Metalloproteinases
keywords: Cell growth; Cell migration; Chronic myeloid leukemia; Hiwi; Matrix metalloproteinases