title: [Control levels of Sin3 histone deacetylase for spontaneous and UV-induced mutagenesis in yeasts Saccharomyces cerevisiae].
abstract: SIN3 gene product operates as a repressor for a huge amount of genes in Saccharomyces cerevisiae. Sin3 protein with a mass of about 175 kDa is a member of the RPD3 protein complex with an assessed mass of greater than 2 million Da. It was previously shownthat RPD3 gene mutations influence recombination and repair processes in S. cerevisiae yeasts. We studied the impacts of the sin3 mutation on UV-light sensitivity and UV-induced mutagenesis in budding yeast cells. The deletion ofthe SIN3 gene causes weak UV-sensitivity of mutant budding cells as compared to the wild-type strain. These results show that the sin3 mutation decreases both spontaneous and UV-induced levels of levels. This fact is hypothetically related to themalfunction of ribonucleotide reductase activity regulation, which leads to a decrease in the dNTP pool and the inaccurate error-prone damage bypass postreplication repair pathway, which in turn provokes a reduction in the incidence of mutations.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Lebovka
authors.forename: T N
authors.initials: TN
authors.lastname: Kozhina
authors.forename: I V
authors.initials: IV
authors.lastname: Fedorova
authors.forename: V T
authors.initials: VT
authors.lastname: Peshekhonov
authors.forename: T A
authors.initials: TA
authors.lastname: Evstiukhina
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Chernenkov
authors.forename: V G
authors.initials: VG
authors.lastname: Korolev
mesh_terms: D004260:DNA Repair; D004261:DNA Replication / Q000235:genetics / Q000528:radiation effects*; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D006655:Histone Deacetylases / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics / Q000528:radiation effects; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: D012097:Repressor Proteins; C071731:SIN3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006655:Histone Deacetylases