title: [Long-term manure application induced shift of diversity and abundance of antibiotic resistance genes in paddy soil].
abstract: To investigate the effect of long-term manure application on the profiles of antibiotic resistance genes (ARGs) in paddy soil, the abundance of ARGs targeted by 295 primers sets were measured by using high-throughput quantitative PCR technique. A total of 107 types of ARGs were detected in long-term manured paddy soil, which is significantly higher than that in control paddy soil (P < 0.05). PCA analysis and UPGMA cluster demonstrated that long-term manure application significantly changed the ARGs diversity and abundance in paddy soils. There were 49 types antibiotic resistance genes were enriched significantly in paddy soil applied with manure (P < 0.05), especially the mexF, which belongs to quinolones-chloramphenicol resistance genes, increased 1791-fold compared with paddy soil of no manure application. Our results showed that long-term application of manure in paddy soil resulted in the shift of abundance and diversity of ARGs, high-throughput quantitative PCR could be a powerful tool concerning the environmental ARGs dimensions.
authors.forename: Fu-Yi
authors.initials: FY
authors.lastname: Huang
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.forename: Bei
authors.initials: B
authors.lastname: Wei
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Ouyang
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Su
mesh_terms: D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D008372:Manure; D012275:Oryza; D012987:Soil; D012988:Soil Microbiology
chemical_list: D008372:Manure; D012987:Soil