title: Lysosomal-associated protein transmembrane 4 Beta-35 overexpression is a novel independent prognostic marker for gastric carcinoma.
abstract: OBJECTIVE
  The purpose of this work was to analyze the relationships between the expression status of Lysosomal-associated protein transmembrane-4 beta 35 (LAPTM4B-35) in cancerous tissues and clinicopathological characteristics and prognosis of the patients with gastric carcinoma (GC).
  
  
  METHODS
  The GC samples from 157 patients in a discovery cohort and 148 patients in a testing cohort with follow-up data were used to validate the feasibility of expression of LAPTM4B-35 protein in predicting GC prognosis. Immunohistochemical staining was used to determine the expression of LAPTM4B-35 protein in precancerous gastric lesions and gastric carcinomas. The correlation between the expression of LAPTM4B-35 and clinicopathologic characteristics of patients with gastric carcinoma was analyzed using chi-square test. Univariate and multivariate analyses were performed to determine the association between LAPTM4B-35 expression and prognosis.
  
  
  RESULTS
  LAPTM4B-35 expression was increased steadily in sequential stages of precancerous gastric lesions. Positive LAPTM4B-35 expression was more frequently detected in patients with distant metastasis (P = 0.023) and III+IV TNM stages (P = 0.042) in the discovery cohort. Kaplan-Meier survival curves and univariate analysis showed that expression of LAPTM4B-35 had a significant impact on overall survival of patients with gastric carcinoma in discovery cohort (P<0.001) and testing cohort (P = 0.001). LAPTM4B-35 expression was an independent prognostic indicator for the overall survival of patients with gastric carcinoma in both cohorts.
  
  
  CONCLUSIONS
  The present research demonstrated that LAPTM4B-35 over-expression was an independent factor in gastric carcinoma prognosis. LAPTM4B gene may be a useful target of interventions slowing the progression of precancerous gastric lesions and a new therapy method to improve the prognosis of gastric carcinoma.
authors.affiliation: Department of Pathology, Binzhou Medical University, Yantai, Shandong Province, China; Department of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan, Shandong Province, China.
authors.forename: Luying
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathology, Binzhou Medical University, Yantai, Shandong Province, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pathology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan, Shandong Province, China.
authors.forename: Gengyin
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Binzhou Medical University, Yantai, Shandong Province, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Pathology, Binzhou Medical University, Yantai, Shandong Province, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Rouli
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D005757:Gastritis, Atrophic / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008679:Metaplasia; D008875:Middle Aged; D015513:Oncogene Proteins / Q000378:metabolism*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C477083:LAPTM4B protein, human; D008565:Membrane Proteins; D015513:Oncogene Proteins