title: Screening and profiling stilbene-type natural products with angiotensin-converting enzyme inhibitory activity from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder.
abstract: In this study, we screened 10 resveratrol derivatives isolated from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder (ABH) for angiotensin I converting enzyme (ACE) inhibitory (ACEI) activity. Among these compounds, (+)-hopeaphenol and (+)-vitisin A showed the lowest IC50 values (∼ 1.5 μM) toward ACE. In addition, the compounds' abundances and distributions in ABH were profiled using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Interestingly, trimers and tetramers of resveratrol were mainly obtained from the bark of ABH when 90% ethanol was used for extraction. This result implies that the antihypertension effect of ABH extract may be mainly contributed by (+)-hopeaphenol (F1) and (+)-vitisin A (F2) in the ABH bark due to their remarkable ACE inhibitions. Moreover, the sizes and structures of these compounds were further correlated to their affinities toward ACE using molecular docking calculations. The results showed that resveratrol tetramers interact with ACE more favorably than other smaller oligomers.
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Pei-Shiuan
authors.initials: PS
authors.lastname: Su
authors.affiliation: Department of Medicinal Chemistry and Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, United States.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Doerksen
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Sin-Hong
authors.initials: SH
authors.lastname: Chen
authors.affiliation: Vaccine Research and Development Center, National Health Research Institutes, Miaoli 35053, Taiwan.
authors.forename: Wang-Chou
authors.initials: WC
authors.lastname: Sung
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Juan
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Reynetha D S
authors.initials: RD
authors.lastname: Rawendra
authors.affiliation: Department of Life Science, National Taitung University, Taitung 95002, Taiwan.
authors.forename: Chiy-Rong
authors.initials: CR
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Li
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Department of Food Science, Faculty of Agricultural Technology, University of Brawijaya, Malang 65145, Indonesia.
authors.lastname: Aisha
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Tzou-Chi
authors.initials: TC
authors.lastname: Huang
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
authors.forename: Ming-Huei
authors.initials: MH
authors.lastname: Liao
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address: changchii@mail.npust.edu.tw.
authors.forename: Chi-I
authors.initials: CI
authors.lastname: Chang
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Research Center for Tropic Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address: jlhsu@mail.npust.edu.tw.
authors.forename: Jue-Liang
authors.initials: JL
authors.lastname: Hsu
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001688:Biological Products / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002853:Chromatography, Liquid / Q000379:methods; D020128:Inhibitory Concentration 50; D062105:Molecular Docking Simulation; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D001688:Biological Products; D013267:Stilbenes; D000077185:Resveratrol
keywords: Ampelopsis brevipedunculata var. hancei; Angiotensin I converting enzyme (ACE); LC–MS/MS; Molecular docking; Oligostilbenes