title: New cancer suppressor gene for colorectal adenocarcinoma: filamin A.
abstract: OBJECTIVE
  To determine the expression and significance of filamin A (FLNa) in colorectal adenocarcinoma tissue.
  
  
  METHODS
  The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and its relationship with clinical parameters and prognosis was analyzed.
  
  
  RESULTS
  The positive expression of FLNa in cancer tissues was lower than that in normal mucosa, and the difference was statistically significant. The expression of FLNa correlated with liver metastasis, lymph node metastasis and rectal invasion depth, regardless of sex, age, tumor location, tumor size, gross shape and histological type of colorectal carcinoma. Multivariate analysis showed that FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma. Moreover, survival analysis showed that the expression level of FLNa was closely related with survival of patients with colorectal adenocarcinoma. The results of RT-PCR and Western blotting were consistent with those of immunohistochemistry.
  
  
  CONCLUSIONS
  FLNa showed low expression in colorectal adenocarcinoma, high correlation with the incidence and development of colorectal cancer, and was considered an indicator of prognosis.
authors.affiliation: Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Zi-Qiang
authors.initials: ZQ
authors.lastname: Tian
authors.affiliation: Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Shi
authors.affiliation: Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Wang
authors.affiliation: Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Gui-Ying
authors.initials: GY
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D016009:Chi-Square Distribution; D003082:Colectomy; D003110:Colonic Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D015536:Down-Regulation; D005260:Female; D064448:Filamins / Q000032:analysis / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D016896:Treatment Outcome; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; C578663:FLNA protein, human; D064448:Filamins
keywords: Colorectal carcinoma; Filamin A; Immunohistochemistry; Reverse transcription-polymerase chain reaction; Western blot