title: Microwave-assisted solid-phase peptide synthesis of neurosecretory protein GL composed of 80 amino acid residues.
abstract: We recently identified a novel cDNA encoding a small secretory protein of 80 amino acid residues, termed neurosecretory protein GL (NPGL), from the chicken hypothalamus. Homologs of NPGL have been reported to be present in mammals, such as human and rat. NPGL is amidated at its C-terminus, contains an intramolecular disulfide bond, and is hydrophobic in nature. In this study, we have optimized the synthesis of the entire 80-amino acid peptide sequence of rat NPGL by microwave-assisted solid-phase peptide synthesis. NPGL was obtained with a 10% yield when the coupling reactions were performed using 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium-3-oxid hexafluorophosphate (HATU) at 50 °C for 5 min, and Fmoc deprotections were performed using 40% piperidine containing 0.1 M HOBt. Furthermore, the disulfide bond of NPGL was formed with 20% yield with the use of glutathione-containing redox buffer and 50% acetonitrile.
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Masuda
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Ooyama
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Shikano
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kondo
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Furumitsu
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Eiko
authors.initials: E
authors.lastname: Iwakoshi-Ukena
authors.affiliation: Section of Behavioral Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ukena
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D006801:Humans; D008872:Microwaves; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis*; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis*; D051381:Rats; D060327:Solid-Phase Synthesis Techniques / Q000379:methods
chemical_list: D000596:Amino Acids; D009419:Nerve Tissue Proteins; D010455:Peptides; C000604721:neurosecretory protein GL, rat
keywords: NPGL; disulfide bond formation; microwave; small protein; solid-phase peptide synthesis