title: Contemporary proteomic strategies for clinical epigenetic research and potential impact for the clinic.
abstract: Novel proteomic methods are revealing the intricacy of the epigenetic landscape affecting gene regulation and improving our knowledge of the pathogenesis of complex diseases. Despite the enormous amount of data regarding epigenetic modifications present in DNA and histones, deciphering their biological relevance in the context of the disease and health is currently still an ongoing process. Here, we consider the relationship between epigenetic research in tumorigenesis and the prospect of knowledge transfer to clinical use, focusing primarily on the epigenetic histone post-translational modifications, which could be used as biomarkers. We additionally focus on the use of proteomic techniques in research and evaluate their usefulness in clinical setting.
authors.affiliation: Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.
authors.forename: Petra
authors.initials: P
authors.lastname: Hudler
authors.forename: Alja
authors.initials: A
authors.lastname: Videtič Paska
authors.forename: Radovan
authors.initials: R
authors.lastname: Komel
mesh_terms: D035843:Biomedical Research / Q000379:methods; D063646:Carcinogenesis / Q000378:metabolism; D044127:Epigenesis, Genetic; D006657:Histones / Q000378:metabolism; D006801:Humans; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods
chemical_list: D006657:Histones
keywords: DNA methylation; cancer; diagnostics; histone modification; immunohistochemistry; mass spectrometry; post-translational modifications; translation; validation