title: Directional Notch trafficking in Sara endosomes during asymmetric cell division in the spinal cord.
abstract: Asymmetric division of neural precursor cells contributes to the generation of a variety of neuronal types. Asymmetric division is mediated by the asymmetric inheritance of fate determinants by the two daughter cells. In vertebrates, asymmetric fate determinants, such as Par3 and Mib, are only now starting to be identified. Here we show that, during mitosis of neural precursors in zebrafish, directional trafficking of Sara endosomes to one of the daughters can function as such a determinant. In asymmetric lineages, where one daughter cell becomes a neuron (n cell) whereas the other divides again to give rise to two neurons (p cell), we found that the daughter that inherits most of the Sara endosomes acquires the p fate. Sara endosomes carry an endocytosed pool of the Notch ligand DeltaD, which is thereby itself distributed asymmetrically. Sara and Notch are both essential for cell fate assignation within asymmetric lineages. Therefore, the Sara endosome system determines the fate decision between neuronal differentiation and mitosis in asymmetric lineages and thereby contributes to controlling the number of neural precursors and differentiated neurons during neurogenesis in a vertebrate.
authors.affiliation: Department of Biochemistry, Sciences II, 30 Quai Ernest-Ansermet, CH 1211 Geneva 4, Switzerland.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kressmann
authors.affiliation: Department of Biochemistry, Sciences II, 30 Quai Ernest-Ansermet, CH 1211 Geneva 4, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Campos
authors.affiliation: Department of Biochemistry, Sciences II, 30 Quai Ernest-Ansermet, CH 1211 Geneva 4, Switzerland.
authors.forename: Irinka
authors.initials: I
authors.lastname: Castanon
authors.affiliation: Institut de Biologie de Valrose, CNRS UMR7277, INSERM 1091, University of Nice-Sophia Antipolis, F-6108 Nice, France.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Fürthauer
authors.affiliation: Department of Biochemistry, Sciences II, 30 Quai Ernest-Ansermet, CH 1211 Geneva 4, Switzerland.
authors.forename: Marcos
authors.initials: M
authors.lastname: González-Gaitán
mesh_terms: D000818:Animals; D060049:Asymmetric Cell Division; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D002465:Cell Movement; D004625:Embryo, Nonmammalian; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D021381:Protein Transport; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D029961:Zebrafish Proteins; C468126:dld protein, zebrafish