title: ¹¹³Cd NMR experiments reveal an unusual metal cluster in the solution structure of the yeast splicing protein Bud31p.
abstract: Establishing the binding topology of structural zinc ions in proteins is an essential part of their structure determination by NMR spectroscopy. Using (113)Cd NMR experiments with (113)Cd-substituted samples is a useful approach but has previously been limited mainly to very small protein domains. Here we used (113)Cd NMR spectroscopy during structure determination of Bud31p, a 157-residue yeast protein containing an unusual Zn3Cys9 cluster, demonstrating that recent hardware developments make this approach feasible for significantly larger systems.
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH (UK).
authors.forename: Anne-Marie M
authors.initials: AM
authors.lastname: van Roon
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Yang
authors.forename: Daniel
authors.initials: D
authors.lastname: Mathieu
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nagai
authors.forename: David
authors.initials: D
authors.lastname: Neuhaus
mesh_terms: D002104:Cadmium / Q000737:chemistry; D007554:Isotopes; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry; D055672:Static Electricity; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
chemical_list: C489248:BUD31 protein, S cerevisiae; D007554:Isotopes; D008670:Metals; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions; D002104:Cadmium; D015032:Zinc
keywords: NMR spectroscopy; heteronuclear correlation; metal clusters; structure elucidation; zinc finger proteins