title: CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models.
abstract: Fluorescent imaging of colorectal tumor cells would improve tumor localization and allow intra-operative staging, facilitating stratification of surgical resections thereby improving patient outcomes. We aimed to develop and test fluorescent nanoparticles capable of allowing this in vivo. Dye-doped silica nanoparticles were synthesized. Anti-CEA (carcinoembryonic antigen) or control IgGs were conjugated to nanoparticles using various chemical strategies. Binding of CEA-targeted or control nanoparticles to colorectal cancer cells was quantified in vitro, and in vivo after systemic-delivery to murine xenografts. CEA-targeted, polyamidoamine dendrimer-conjugated, nanoparticles, but not control nanoparticles, allowed strong tumor-specific imaging. We are the first to demonstrate live, specific, in vivo imaging of colorectal cancer cells using antibody-targeted fluorescent nanoparticles. These nanoparticles have potential to allow intra-operative fluorescent visualization of tumor cells.
authors.affiliation: School of Medicine, University of Leeds, Leeds, LS9 7TF, UK.
authors.forename: James P
authors.initials: JP
authors.lastname: Tiernan
authors.forename: Nicola
authors.initials: N
authors.lastname: Ingram
authors.forename: Gemma
authors.initials: G
authors.lastname: Marston
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Perry
authors.forename: Jo V
authors.initials: JV
authors.lastname: Rushworth
authors.forename: P Louise
authors.initials: PL
authors.lastname: Coletta
authors.forename: Paul A
authors.initials: PA
authors.lastname: Millner
authors.forename: David G
authors.initials: DG
authors.lastname: Jayne
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Hughes
mesh_terms: D000818:Animals; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D045744:Cell Line, Tumor; D003106:Colon / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods; D012007:Rectum / Q000473:pathology; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D012822:Silicon Dioxide
keywords: IVIS imaging; colorectal cancer; dye-doped; live tumor imaging; silica nanoparticles; tumor markers