title: [Morphology of NCTC cells upon a contact with type I collagen added to culture medium].
abstract: The morphometric characteristics of NCTC cells upon their contact with type I collagen added to culture medium were studied. The cells were plated on plastic in the colony form. In a day after seeding, the culture medium was changed for the same medium complemented with 0.1% type I collagen. And the cells were incubated in this medium for 30 min more. Then the cells were washed out of collagen. Using time-lapse microscopy, the cell state at a colony edge was registered during 7 h. The area, spreading, and polarization of the cells were evaluated. It is shown that the contact with collagen did not affect the cell area, decreased cell spreading and sharply reduced a portion of polarized cells. These results probably demonstrate sensitivity of NCTC cells to the presence of type I collagen in culture medium and suggest that the cell response involves inhibition of long filopodia formation.
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Petrov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Neguliaev
authors.forename: N V
authors.initials: NV
authors.lastname: Tsupkina
mesh_terms: D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D016764:Cell Polarity; D024042:Collagen Type I / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D011554:Pseudopodia / Q000187:drug effects* / Q000648:ultrastructure; D059008:Time-Lapse Imaging
chemical_list: D024042:Collagen Type I; D003470:Culture Media