title: Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines.
abstract: Breast cancer is a major form of cancer, with a high mortality rate in women. It is crucial to achieve more efficient and safe anticancer drugs. Recent developments in medical nanotechnology have resulted in novel advances in cancer drug delivery. Cisplatin, doxorubicin, and 5-fluorouracil are three important anti-cancer drugs which have poor water-solubility. In this study, we used cisplatin, doxorubicin, and 5-fluorouracil-loaded polycaprolactone-polyethylene glycol (PCL-PEG) nanoparticles to improve the stability and solubility of molecules in drug delivery systems. The nanoparticles were prepared by a double emulsion method and characterized with Fourier Transform Infrared (FTIR) spectroscopy and Hydrogen-1 nuclear magnetic resonance ((1)HNMR). Cells were treated with equal concentrations of cisplatin, doxorubicin and 5-fluorouracil-loaded PCL-PEG nanoparticles, and free cisplatin, doxorubicin and 5-fluorouracil. The 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) assay confirmed that cisplatin, doxorubicin, and 5-fluorouracil-loaded PCL-PEG nanoparticles enhanced cytotoxicity and drug delivery in T47D and MCF7 breast cancer cells. However, the IC50 value of doxorubicin was lower than the IC50 values of both cisplatin and 5-fluorouracil, where the difference was statistically considered significant (p˂0.05). However, the IC50 value of all drugs on T47D were lower than those on MCF7.
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.|c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Eatemadi
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Darabi
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, International Branch Aras , Tabriz , Iran.
authors.forename: Loghman
authors.initials: L
authors.lastname: Afraidooni
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, International Branch Aras , Tabriz , Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hadis
authors.initials: H
authors.lastname: Daraee
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Eskandari
authors.affiliation: c Department of Clinical Biochemistry , Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mellatyar
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, International Branch Aras , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D005472:Fluorouracil / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; D004317:Doxorubicin; D002945:Cisplatin; C000595209:polyethylene glycol 1000; D005472:Fluorouracil
keywords: 5-Fluorouracil; anti-cancer drugs; breast cancer; cisplatin; doxorubicin