title: Morphometry of the trabecular meshwork in vivo in a healthy population using fourier-domain optical coherence tomography.
abstract: OBJECTIVE
  We measured the length, thickness, and area of the trabecular meshwork (TM) in vivo using Fourier domain optical coherence tomography (FD-OCT) in a Caucasian population of healthy subjects.
  
  
  METHODS
  A cross-sectional study was done of 1006 healthy subjects. Left eyes were randomly selected. Age, sex, IOP, and spherical refractive error were noted. The depth and volume of the anterior chamber and the central corneal thickness were measured with Pentacam, while IOL Master was used to measure the axial length. The length, thickness, and area of the TM were measured through FD-OCT RTVue. A study was done to determine the correlation between TM size, and other demographic and ocular parameters. Finally, the reproducibility of the measurements was assessed for a subgroup of 50 eyes from 50 patients.
  
  
  RESULTS
  We were able to measure the TM in 91.1% of the total eyes studied. The mean TM length was 496.99 ± 92.77 μm (range, 275-800), TM thickness was 174.16 ± 28.14 μm (range, 100-276), and TM area was 0.069 ± 0.031 mm(2) (range, 0.023-0.133). No differences were found in terms of length and area for sex, although the TM was slightly thicker in men (P = 0.046). No correlation was observed between the TM measurements and any of the studied demographic or ocular parameters (R < 0.09, P < 0.001). Intra- and interobserver reproducibility of the measurements were good (intraclass correlation coefficient > 0.750, P < 0.001).
  
  
  CONCLUSIONS
  The FD-OCT is an effective and reproducible examination technique to measure the length, thickness, and area of the TM in vivo.
authors.affiliation: Clínico San Carlos University Hospital, Department of Ophthalmology, Institute of Health Research, Complutense University of Madrid, Madrid, Spain International Center of Advanced Ophthalmology, Madrid, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: Fernández-Vigo
authors.affiliation: Clínico San Carlos University Hospital, Department of Ophthalmology, Institute of Health Research, Complutense University of Madrid, Madrid, Spain.
authors.forename: Julián
authors.initials: J
authors.lastname: García-Feijóo
authors.affiliation: Clínico San Carlos University Hospital, Department of Ophthalmology, Institute of Health Research, Complutense University of Madrid, Madrid, Spain.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Martínez-de-la-Casa
authors.affiliation: Clínico San Carlos University Hospital, Department of Ophthalmology, Institute of Health Research, Complutense University of Madrid, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: García-Bella
authors.affiliation: International Center of Advanced Ophthalmology, Madrid, Spain Department of Ophthalmology, University of Extremadura, Badajoz, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Fernández-Vigo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003430:Cross-Sectional Studies; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D041623:Tomography, Optical Coherence; D014129:Trabecular Meshwork / Q000033:anatomy & histology*; D055815:Young Adult
keywords: glaucoma anterior segment; optical coherence tomography; trabecular meshwork