title: Evaluation of testicular tissue of adult rats treated with cisplatin incorporated into the liposome.
abstract: Cisplatin (CPL) is one of the most widely used and effective chemotherapeutic agents for the treatment of several human malignancies. However, it causes serious side effects, especially on reproduction. In order to reduce the undesirable effects caused by many drugs, liposomes have been used as a good system for drug delivery. The aim of this study was to investigate, for the first time, the effects of CPL incorporated into the dipalmitoyl phosphatidylcholine liposome (DPPC) on the testicular tissue of adult Wistar rats. The animals (n = 20) were distributed into four experimental groups: (a) control (distillated water); (b) liposome (DPPC, 1 mL), (c) cisplatin incorporated into liposome (CPL/DPPC), and (d) CPL (8 mg/kg body weight). The animals received a single intraperitoneal injection and were killed 10 days after each treatment for histopathological analysis of testes. The results showed that the testicular histomorphometric parameters in rats of DPPC and CPL/DPPC groups were similar to those of the control group. Meanwhile, rats of the CPL-treated group showed a variety of morphological alterations, including atrophy of seminiferous tubules and presence of multinucleated cells in the germinal epithelium. The incorporation of CPL into the liposome had no influence on the testicular weight or any other stereological parameters, but it was beneficial in maintaining the body weight of the animals. In conclusion, the liposome suppressed the cytotoxic effects caused by cisplatin in the testes of rats, suggesting a possible use in chemotherapy against cancer to reduce the side effects seen on reproduction.
authors.affiliation: Graduate Program in Environment and Regional Development, University of Western São Paulo - UNOESTE, Presidente Prudente, São Paulo, Brazil.
authors.forename: Ana Paula Alves
authors.initials: AP
authors.lastname: Favareto
authors.forename: Lázaro Cícero
authors.initials: LC
authors.lastname: Nogueira
authors.forename: Davi Abeid
authors.initials: DA
authors.lastname: Pontes
authors.forename: SuZana de Fátima Paccola
authors.initials: Sde F
authors.lastname: Mesquita
authors.forename: Isabel Cristina Cherici
authors.initials: IC
authors.lastname: Camargo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000009:adverse effects*; D002945:Cisplatin / Q000008:administration & dosage* / Q000009:adverse effects*; D004337:Drug Carriers / Q000008:administration & dosage*; D006651:Histocytochemistry; D007274:Injections, Intraperitoneal; D008081:Liposomes / Q000008:administration & dosage*; D008297:Male; D008853:Microscopy; D017208:Rats, Wistar; D013737:Testis / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008081:Liposomes; D002945:Cisplatin
keywords: chemotherapeutic agent; reproduction; spermatogenesis; synthetic vesicles