title: The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent.
abstract: Bone sialoprotein (BSP) belongs to the "small integrin-binding ligand N-linked glycoprotein" (SIBLING) family, whose members interact with bone cells and bone mineral. BSP is strongly expressed in bone and we previously showed that BSP knockout (BSP-/-) mice have a higher bone mass than wild type (BSP+/+) littermates, with lower bone remodelling. Because baseline bone formation activity is constitutively lower in BSP-/- mice, we studied the impact of the absence of BSP on in vitro osteogenesis in mouse calvaria cell (MCC) cultures. MCC BSP-/- cultures exhibit fewer fibroblast (CFU-F), preosteoblast (CFU-ALP) and osteoblast colonies (bone nodules) than wild type, indicative of a lower number of osteoprogenitors. No mineralized colonies were observed in BSP-/- cultures, along with little/no expression of either osteogenic markers or SIBLING proteins MEPE or DMP1. Osteopontin (OPN) is the only SIBLING expressed in standard density BSP-/- culture, at higher levels than in wild type in early culture times. At higher plating density, the effects of the absence of BSP were partly rescued, with resumed expression of osteoblast markers and cognate SIBLING proteins, and mineralization of the mutant cultures. OPN expression and amount are further increased in high density BSP-/- cultures, while PHEX and CatB expression are differentiatlly regulated in a manner that may favor mineralization. Altogether, we found that BSP regulates mouse calvaria osteoblast cell clonogenicity, differentiation and activity in vitro in a cell density dependent manner, consistent with the effective skeletogenesis but the low levels of bone formation observed in vivo. The BSP knockout bone microenvironment may alter the proliferation/cell fate of early osteoprogenitors.
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Guenaelle
authors.initials: G
authors.lastname: Bouet
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Wafa
authors.initials: W
authors.lastname: Bouleftour
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Juignet
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Linossier
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Thomas
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Vanden-Bossche
authors.affiliation: Dept. of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Aubin
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Vico
authors.affiliation: Ecole Nationale Supérieure des Mines de Saint-Etienne, Center for Health Engineering, SFR IFRESIS, Saint-Etienne, France.
authors.forename: David
authors.initials: D
authors.lastname: Marchat
authors.affiliation: INSERM U1059, Laboratoire de Biologie du Tissu Osseux, SFR IFRESIS, Université de Lyon and Université Jean Monnet, St-Etienne, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Malaval
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002401:Cathepsin B / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010012:Osteogenesis; D053495:Osteopontin / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D053402:PHEX Phosphate Regulating Neutral Endopeptidase / Q000378:metabolism; D012886:Skull / Q000166:cytology* / Q000378:metabolism
chemical_list: C506896:Spp1 protein, mouse; D053495:Osteopontin; D002401:Cathepsin B; D053402:PHEX Phosphate Regulating Neutral Endopeptidase; C506232:Phex protein, mouse