title: Laminin receptor protein is implicated in hemocyte homeostasis for the whiteleg shrimp Penaeus (Litopenaeus) vannamei.
abstract: Here we show that knockdown of laminin receptor (Lamr) with PvLamr dsRNA in the whiteleg shrimp Penaeus (Litopenaeus) vannamei (Pv) caused a dramatic reduction specifically in hyaline hemocytes prior to death. Since apoptosis was not detected in hemocytes or hematopoietic cells, other possible causes of hemocyte loss were investigated. Reports that suppression of crustacean hematopoietic factor (CHF)-like protein or hemocyte homeostasis-associated protein (HHAP) also reduced shrimp hemocyte counts led us to carry out yeast two-hybrid (Y2H) and co-immunoprecipitation (co-IP) assays to test for interactions between Lamr and Pv homologues to these proteins (PvCHF-like and PvHHAP). The assays revealed that Lamr bound to both these homologues, but that the homologues did not bind to each other. Subsequent RT-PCR assays confirmed that PvLamr dsRNA injection significantly reduced expression levels for both PvCHF-like and PvHHAP genes. Further work is needed to determine how interaction among these three proteins can regulate shrimp hemocyte homeostasis.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Walaiporn
authors.initials: W
authors.lastname: Charoensapsri
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand.
authors.forename: Pakkakul
authors.initials: P
authors.lastname: Sangsuriya
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Tareerat
authors.initials: T
authors.lastname: Lertwimol
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Warachin
authors.initials: W
authors.lastname: Gangnonngiw
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Kornsunee
authors.initials: K
authors.lastname: Phiwsaiya
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand. Electronic address: saengchan@biotec.or.th.
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics / Q000378:metabolism; D006434:Hemocytes / Q000502:physiology*; D006706:Homeostasis / Q000235:genetics; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000276:immunology*; D011485:Protein Binding / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D016298:Hematopoietic Cell Growth Factors; D034741:RNA, Small Interfering; D017474:Receptors, Laminin
keywords: Apoptosis; Hemocyte; Homeostasis; Laminin receptor; RNAi; Shrimp