title: [Extracellular proteasomes purification methods and the evaluation of proteasomal peptidase activities].
abstract: In this paper, we present a comparative analysis of different methods of purification of proteasomes from the culture medium in which proerithroleukemia human K562 cells were grown. The results obtained allowed us to purify proteasomes from samples of conditioned cell culture medium and control the quality of the proteasome preparations at all stages of their separation. Extracellular proteasomes purified via different approaches possess all the three types of peptidase activity described for intracellular counterparts.
authors.forename: Iu Ia
authors.initials: IuIa
authors.lastname: Zaĭkova
authors.forename: V A
authors.initials: VA
authors.lastname: Kulichkova
authors.forename: K A
authors.initials: KA
authors.lastname: Mitenkova
authors.forename: A S
authors.initials: AS
authors.lastname: Tsimokha
mesh_terms: D020169:Caspases / Q000737:chemistry / Q000302:isolation & purification*; D002918:Chymotrypsin / Q000737:chemistry / Q000302:isolation & purification*; D017077:Culture Media, Conditioned / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D059748:Proteolysis; D014357:Trypsin / Q000737:chemistry / Q000302:isolation & purification*; D054875:Ubiquitination
chemical_list: D017077:Culture Media, Conditioned; D002918:Chymotrypsin; D014357:Trypsin; D020169:Caspases; D046988:Proteasome Endopeptidase Complex