title: Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo.
abstract: Quantification of the number of infectious viruses in a sample is a basic virological technique. In this chapter we provide a detailed description of three techniques to estimate the number of viable infectious avian coronaviruses in a sample. All three techniques are serial dilution assays, better known as titrations.
authors.affiliation: Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands, joeri.kint@wur.nl.
authors.forename: Joeri
authors.initials: J
authors.lastname: Kint
authors.forename: Helena Jane
authors.initials: HJ
authors.lastname: Maier
authors.forename: Erik
authors.initials: E
authors.lastname: Jagt
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus / Q000302:isolation & purification* / Q000502:physiology; D011201:Poultry Diseases / Q000821:virology*; D017186:Titrimetry; D019562:Viral Load; D010948:Viral Plaque Assay; D014776:Virus Cultivation