title: Cell surface receptors: rapid quantification of live cell receptors using bioluminescence in a flow-based microfluidic device (small 8/2015).
abstract: C. T. Lim and co-workers describe a rapid and sensitive bioluminescence-based microfluidic method for quantifying receptor numbers on live cells. On page 943, this integrated, lens-free optical platform allows the determination of signals from the cell surface with high sensitivity. Compared to conventional approaches, the combined use of bioluminescence and microfluidics makes it safe to use, reduces background noise, improves sensitivity, requires smaller sample volumes, and allows high-throughput sampling over thousands of cells.
authors.affiliation: Department of Biomedical Engineering, 9 Engineering Drive 1, Block EA, #03-12, National University of Singapore, Singapore, 117575.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ramji
authors.forename: Cheong Fook
authors.initials: CF
authors.lastname: Cheong
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hirata
authors.forename: Abdur Rub Abdur
authors.initials: AR
authors.lastname: Rahman
authors.forename: Chwee Teck
authors.initials: CT
authors.lastname: Lim
keywords: cell islands; cell surface receptors; gaussia luciferase; lens-free optics; microfluidics; receptor numbers