title: Comparison of urinary levels of 8-hydroxy-2'-deoxyguanosine between young females with and without depressive symptoms during different menstrual phases.
abstract: This study aimed to clarify the association between depressive symptoms and a marker of oxidative stress-induced DNA damage in young females. Since the menstrual cycle may confound or modify this association, depressive symptoms and urinary levels of 8-hydroxy-2' deoxyguanosine (8-OHdG) were evaluated during each menstrual phase. A total of 57 female fourth-year students (aged 21.6±0.8) from a Japanese health science university were studied. The menstrual cycle was divided into 3 phases:menstrual (days 1 to 3 after the onset of menses);proliferative (days 13 to 15);and secretory (days 24 to 26). Depressive symptoms were assessed by the self-rating depression scale (SDS). Positive depressive symptoms were defined as a score of 53 or more during 2 different menstrual phases. The association between the presence of depressive symptoms and 8-OHdG levels adjusting for the menstrual cycle was examined by two-way analysis of variance with the menstrual cycle (menstrual, proliferative, and secretory phases) as the within-individual factor. The menstrual cycle did not show a significant correlation with urinary 8-OHdG levels. On the other hand, the menstrual cycle-adjusted 8-OHdG level was significantly higher in those with depressive symptoms (7.01ng/mL) than in those without them (3.98ng/mL). The ROC curve analysis showed that urinary 8-OHdG levels had reasonably high discriminative performance throughout all the menstrual cycles (0.73-0.81;all p＜0.05). These results indicated the presence of oxidative stress in subjects with depressive symptoms independent of the menstrual cycle.
authors.affiliation: Department of Physical Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima, Mihara, Hiroshima 723-0053, Japan.
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Iida
authors.forename: Ken
authors.initials: K
authors.lastname: Inoue
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Miwa
authors.initials: M
authors.lastname: Kagiono
authors.forename: Ryoji
authors.initials: R
authors.lastname: Teradaira
authors.forename: Chiho
authors.initials: C
authors.lastname: Chikamura
authors.forename: Toshihide
authors.initials: T
authors.lastname: Harada
authors.forename: Satoko
authors.initials: S
authors.lastname: Ezoe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yatsuya
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D003863:Depression / Q000652:urine*; D005260:Female; D006801:Humans; D008597:Menstrual Cycle / Q000378:metabolism*; D012372:ROC Curve; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine