title: Serum metabolomics reveals that arsenic exposure disrupted lipid and amino acid metabolism in rats: a step forward in understanding chronic arsenic toxicity.
abstract: Chronic arsenic exposure through drinking water threatens public health worldwide. Although its multiorgan toxicity has been reported, the impact of chronic arsenic exposure on the metabolic network remains obscure. In this study, male Sprague Dawley rats were exposed to 0.5, 2 or 10 ppm sodium arsenite for three months. An ultra-high performance liquid chromatography/mass spectrometry based metabolomics approach was utilized to unveil the global metabolic response to chronic arsenic exposure in rats. Distinct serum metabolome profiles were found to be associated with the doses. Eighteen differential metabolites were identified, and most of them showed dose-dependent responses to arsenic exposure. Metabolic abnormalities mainly involved lipid metabolism and amino acid metabolism. The metabolic alterations were further confirmed by hepatic gene expression. Expressions of cpt2, lcat, cact, crot and mtr were significantly elevated in high dose groups. This study provides novel evidence to support the association between arsenic exposure and metabolic disruption, and it contributes to understanding the mechanism of chronic arsenic toxicity.
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, P. R. China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Mu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Huang
authors.forename: Ambreen
authors.initials: A
authors.lastname: Alamdar
authors.forename: Meiping
authors.initials: M
authors.lastname: Tian
authors.forename: Liangpo
authors.initials: L
authors.lastname: Liu
authors.forename: Heqing
authors.initials: H
authors.lastname: Shen
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D020261:Arsenic Poisoning / Q000097:blood / Q000378:metabolism; D002908:Chronic Disease; D016002:Discriminant Analysis; D004781:Environmental Exposure; D005786:Gene Expression Regulation / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects; D016018:Least-Squares Analysis; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008297:Male; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018675:Toxicity Tests
chemical_list: D000596:Amino Acids; D012333:RNA, Messenger; D001151:Arsenic