title: Heterogeneous CD8+ T cell migration in the lymph node in the absence of inflammation revealed by quantitative migration analysis.
abstract: The three-dimensional positions of immune cells can be tracked in live tissues precisely as a function of time using two-photon microscopy. However, standard methods of analysis used in the field and experimental artifacts can bias interpretations and obscure important aspects of cell migration such as directional migration and non-Brownian walk statistics. Therefore, methods were developed for minimizing drift artifacts, identifying directional and anisotropic (asymmetric) migration, and classifying cell migration statistics. These methods were applied to describe the migration statistics of CD8+ T cells in uninflamed lymph nodes. Contrary to current models, CD8+ T cell statistics are not well described by a straightforward persistent random walk model. Instead, a model in which one population of cells moves via Brownian-like motion and another population follows variable persistent random walks with noise reproduces multiple statistical measures of CD8+ T cell migration in the lymph node in the absence of inflammation.
authors.affiliation: Department of Physics and Astronomy, School of Arts and Sciences, University of Pennsylvania, Philadelphia, Philadelphia, United States of America; Department of Physics and Astronomy, Northwestern University, Evanston, Illinois, United States of America.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Banigan
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Philadelphia, United States of America; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia, United States of America.
authors.forename: Tajie H
authors.initials: TH
authors.lastname: Harris
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Philadelphia, United States of America.
authors.forename: David A
authors.initials: DA
authors.lastname: Christian
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Philadelphia, United States of America.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Hunter
authors.affiliation: Department of Physics and Astronomy, School of Arts and Sciences, University of Pennsylvania, Philadelphia, Philadelphia, United States of America.
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D019295:Computational Biology; D003198:Computer Simulation; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018448:Models, Immunological; D013154:Spleen / Q000166:cytology