title: Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst.
abstract: OBJECTIVE
  To produce 10-ketostearic acid from oleic acid.
  
  
  RESULTS
  Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1).
  
  
  CONCLUSIONS
  This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
authors.forename: Byeonghun
authors.initials: B
authors.lastname: Lee
authors.forename: Saebom
authors.initials: S
authors.lastname: Lee
authors.forename: Hyeonsoo
authors.initials: H
authors.lastname: Kim
authors.forename: Kijun
authors.initials: K
authors.lastname: Jeong
authors.forename: Jinbyung
authors.initials: J
authors.lastname: Park
authors.forename: Eunyeol
authors.initials: E
authors.lastname: Lee
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016982:Micrococcus luteus / Q000201:enzymology / Q000235:genetics; D019301:Oleic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism*; D020615:Stenotrophomonas maltophilia / Q000201:enzymology / Q000235:genetics; D013696:Temperature
chemical_list: C403718:10-ketostearic acid; D011994:Recombinant Proteins; D013229:Stearic Acids; D019301:Oleic Acid; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP); D006836:Hydro-Lyases