title: Label-free quantitative mass spectrometry reveals a panel of differentially expressed proteins in colorectal cancer.
abstract: To identify potential biomarkers involved in CRC, a shotgun proteomic method was applied to identify soluble proteins in three CRCs and matched normal mucosal tissues using high-performance liquid chromatography and mass spectrometry. Label-free protein profiling of three CRCs and matched normal mucosal tissues were then conducted to quantify and compare proteins. Results showed that 67 of the 784 identified proteins were linked to CRC (28 upregulated and 39 downregulated). Gene Ontology and DAVID databases were searched to identify the location and function of differential proteins that were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, and so on. Among the differentially expressed proteins, tropomyosin-3 (TPM3), endoplasmic reticulum resident protein 29 (ERp29), 18 kDa cationic antimicrobial protein (CAMP), and heat shock 70 kDa protein 8 (HSPA8) were verified to be upregulated in CRC tissue and seven cell lines through western blot analysis. Furthermore, the upregulation of TPM3, ERp29, CAMP, and HSPA8 was validated in 69 CRCs byimmunohistochemistry (IHC) analysis. Combination of TPM3, ERp29, CAMP, and HSPA8 can identify CRC from matched normal mucosal achieving an accuracy of 73.2% using IHC score. These results suggest that TPM3, ERp29, CAMP, and HSPA8 are great potential IHC diagnostic biomarkers for CRC.
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, No. 2, Huaxiaxi Road, Luoyang 471000, China ; The General Hospital of Jinan Military Region, Jinan 25000, China.
authors.forename: Nai-Jun
authors.initials: NJ
authors.lastname: Fan
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, No. 2, Huaxiaxi Road, Luoyang 471000, China.
authors.forename: Jiang-Ling
authors.initials: JL
authors.lastname: Gao
authors.affiliation: The Department of Neurosurgery, No. 150 Central Hospital of PLA, Luoyang 471000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, No. 2, Huaxiaxi Road, Luoyang 471000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, No. 2, Huaxiaxi Road, Luoyang 471000, China.
authors.forename: Zhan-Yang
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, No. 2, Huaxiaxi Road, Luoyang 471000, China.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins