title: Genetic polymorphism of ABCB1 gene (C3435T) in patients with inflammatory bowel diseases. Is there any gender dependency?
abstract: BACKGROUND
  In recent years, an increasing incidence of inflammatory bowel disease (IBD) has been reported, mainly as Crohn's disease (CD) and ulcerative colitis (UC). The individual susceptibility, the disease's course and response to the applied therapy is likely due to genetic factors such as ABCB1 gene mutations, exemplified by C3435T polymorphism. The aim of the study was to evaluate the distribution of C3435T polymorphism regarding the gender in IBD patients and control subjects from Lower Silesia region and its possible association with IBD susceptibility.
  
  
  METHODS
  The research was conducted in groups of 61 IBD patients and 101 healthy subjects from the Lower Silesia region. Polymorphism of C3435T was determined using PCR-RFLP method.
  
  
  RESULTS
  Frequency distributions of C3435T genotype and of 3435T or 3435C gene alleles of IBD, CD or UC patients were compared to control group; each treated as a whole or split further by gender. The statistically significant correlation was discovered between gender and C3435T genotype both for IBD and CD patients, with 3435CT heterozygote prevailing in IBD and CD males. Odds ratio calculations revealed statistically significant difference for the 3435CT genotype between control and: IBD group considered as a whole; IBD males; CD males; and for 3435TT variant between control and IBD males. Conclusions. The 3435CT genotype could be a risk factor for IBD and CD in men. The 3435TT genotype in males seems to be associated with the lower chance of IBD presence.
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: tje1@wp.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Jaźwińska-Tarnawska
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Jęśkowiak
authors.affiliation: Department of Gastroenterology and Hepatology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Waszczuk
authors.affiliation: Department of Gastroenterology and Hepatology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Mulak
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: gastro@gastro.umed.wroc.pl.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Głowacka
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Hurkacz
authors.affiliation: Department of Gastroenterology and Hepatology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Paradowski
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Zaleska
authors.affiliation: Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: wf-12@umed.wroc.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Wiela-Hojeńska
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics*; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012727:Sex Characteristics
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B
keywords: ABCB1 gene; Genetic polymorphism; Inflammatory bowel diseases; P-glycoprotein