title: Automated synthesis of arabinoxylan-oligosaccharides enables characterization of antibodies that recognize plant cell wall glycans.
abstract: Monoclonal antibodies that recognize plant cell wall glycans are used for high-resolution imaging, providing important information about the structure and function of cell wall polysaccharides. To characterize the binding epitopes of these powerful molecular probes a library of eleven plant arabinoxylan oligosaccharides was produced by automated solid-phase synthesis. Modular assembly of oligoarabinoxylans from few building blocks was enabled by adding (2-naphthyl)methyl (Nap) to the toolbox of orthogonal protecting groups for solid-phase synthesis. Conjugation-ready oligosaccharides were obtained and the binding specificities of xylan-directed antibodies were determined on microarrays.
authors.affiliation: Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam (Germany); Freie Universität Berlin, Institute of Chemistry and Biochemistry, Arnimallee 22, 14195 Berlin (Germany).
authors.forename: Deborah
authors.initials: D
authors.lastname: Schmidt
authors.forename: Frank
authors.initials: F
authors.lastname: Schuhmacher
authors.forename: Andreas
authors.initials: A
authors.lastname: Geissner
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
authors.forename: Fabian
authors.initials: F
authors.lastname: Pfrengle
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology*; D046228:Microarray Analysis; D059828:Plant Cells / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology; D060327:Solid-Phase Synthesis Techniques; D014990:Xylans / Q000138:chemical synthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D014990:Xylans; C085118:arabinoxylan
keywords: arabinoxylan; carbohydrates; microarray; plant cell wall; solid-phase synthesis