title: Endothelial dysfunction in young healthy men is associated with aspirin resistance.
abstract: The aim of this study was to investigate the relation between endothelial dysfunction and aspirin response in a young healthy population (102 men aged 18-40). Initial concentrations of the NO pathway metabolites (ADMA, l-arginine, SDMA), cardiovascular risk markers, oxidative stress markers (MDA, thiol index), sICAM1, sVCAM1, PAI-1, sE-selectin, sP-selectin, VEGF, thromboxane B2, 6-keto-PGF1α and arachidonate-induced platelet aggregation (to separate aspirin resistant from sensitive group) were measured. Flow-mediated-vasodilation (FMD) was measured before and after intravenous infusion of 16.0 g of l-arginine. Measurements were repeated following aspirin administration (75 mg/24 h) for 4 days. Both groups were homogenous regarding demographic and biochemical characteristics reflecting cardiovascular risk. Aspirin resistant subjects were characterized by lower baseline FMD and higher FMD following aspirin and l-arginine treatment, as compared to aspirin sensitive control. MDA and nitrotyrosine were greater, whereas thiol index was lower in aspirin resistant men. The sICAM1, sVCAM1, PAI-1, sE-selectin, sP-selectin and VEGF levels were similar in the analyzed groups. Thromboxane in aspirin resistant subjects was greater both at baseline and following aspirin therapy. However, a significant decrease following aspirin treatment was present in both groups. Aspirin resistance in young men is associated with endothelial dysfunction, which could be due to oxidative stress resulting from lipid peroxidation.
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland. Electronic address: adrian.doroszko@gmail.com.
authors.forename: Adrian
authors.initials: A
authors.lastname: Doroszko
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Szahidewicz-Krupska
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Janus
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jakubowski
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Turek
authors.affiliation: Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Ilnicka
authors.affiliation: Faculty of Health Science, Wroclaw Medical University, Bartla 5, 51-618 Wrocław, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szuba
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Mazur
authors.affiliation: Department of Internal Medicine, Occupational Diseases and Hypertension, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland; Wrovasc - Integrated Cardiovascular Centre, Co-financed by the European Regional Development Fund, within the Innovative Economy Operational Program, 2007-2013, Provincial Specialized Hospital, Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Derkacz
mesh_terms: D000293:Adolescent; D000328:Adult; D001241:Aspirin / Q000494:pharmacology*; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D004351:Drug Resistance / Q000187:drug effects* / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008297:Male; D012307:Risk Factors; D013931:Thromboxanes / Q000097:blood; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology; D055815:Young Adult
chemical_list: D013931:Thromboxanes; D001241:Aspirin
keywords: Aspirin resistance; Endothelial dysfunction; Platelets