title: Sub-100 nm resolution microscopy based on proximity projection grating scheme.
abstract: Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured illumination microscopy using the proximity projection grating scheme (PPGS), which has the ability to further enhance the SIM resolution without invoking any nonlinearity response from the sample. With the PPGS-based SIM, sub-100 nm resolution has been obtained experimentally, and results corresponding to 2.4 times resolution improvement are presented. Furthermore, it will be shown that an improvement of greater than 3 times can be achieved.
authors.affiliation: Department of Electrical and Electronic Engineering, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Feng
authors.initials: F
authors.lastname: Hu
authors.affiliation: Institute of Biophysics, Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Somekh
authors.affiliation: Institute of Biophysics, Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Albutt
authors.affiliation: Institute of Biophysics, Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Webb
authors.affiliation: Institute of Biophysics, Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Emilia
authors.initials: E
authors.lastname: Moradi
authors.affiliation: Department of Electrical and Electronic Engineering, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Chung W
authors.initials: CW
authors.lastname: See
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D007089:Image Enhancement; D057230:Limit of Detection; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D041681:NIH 3T3 Cells
chemical_list: D005456:Fluorescent Dyes