title: Facile and rapid deprotection conditions for the cleavage of synthetic oligonucleotides from 1,4-anhydroerythritol-based universal polymer support.
abstract: In our previous report [Kumar, P.; Dhawan, G.; Chandra, R.; Gupta, K.C. Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support. Nucl. Acids Res. 2002, 30, e130 (1-8)], we demonstrated polyamine-mediated deprotection of oligonucleotides from cis-diol group bearing universal polymer support (I). However, vulnerability of the conventional dC(bz) to modifications under these conditions compelled us to employ dC(ac) during synthesis of oligonucleotide using conventional synthons. Here, a new set of simple and rapid deprotection conditions has been developed for the complete cleavage of oligonucleotides from the 1,4-anhydroerythritol-based universal polymer support employing conventional dC(bz) synthon. Using manganese-imidazole complex in aqueous ammonium hydroxide (∼ 30%), fully deprotected oligonucleotide sequences were obtained in 40 min, which were analyzed on reverse phase-HPLC and compared with the standard oligomers in terms of their retention time. Finally, their biological compatibility was established by analyzing PCR amplified products of npsA gene of N. meningitidis.
authors.affiliation: a Nucleic Acids Research Laboratory, CSIR-Institute of Genomics and Integrative Biology , Delhi , India.
authors.forename: Gagan
authors.initials: G
authors.lastname: Dhawan
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
authors.forename: Kailash C
authors.initials: KC
authors.lastname: Gupta
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
mesh_terms: D064753:Ammonium Hydroxide; D002851:Chromatography, High Pressure Liquid; D004896:Erythritol / Q000031:analogs & derivatives* / Q000737:chemistry; D006868:Hydrolysis; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: C518865:1,4-anhydroerythritol; D009841:Oligonucleotides; D011073:Polyamines; D011108:Polymers; D064753:Ammonium Hydroxide; D004896:Erythritol
keywords: Oligonucleotides; PCR; RP-HPLC; deprotection conditions; universal polymer support