title: Differential immunodetection of toxin B from highly virulent Clostridium difficile BI/NAP-1/027.
abstract: We developed a simple immunoassay capable of differentially detecting toxin B from highly virulent strains of Clostridium difficile (BI/NAP-1/027) in stool. This assay can simultaneously confirm the presence of in vivo toxin production and provide strain-related information relevant to infection control epidemiology and disease prognosis.
authors.affiliation: Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA Division of Laboratory Medicine, Boston Children's Hospital, Boston, Massachusetts, USA nira.pollock@childrens.harvard.edu.
authors.forename: Nira R
authors.initials: NR
authors.lastname: Pollock
authors.affiliation: Quanterix Corporation, Lexington, Massachusetts, USA.
authors.forename: Linan
authors.initials: L
authors.lastname: Song
authors.affiliation: Quanterix Corporation, Lexington, Massachusetts, USA.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Quanterix Corporation, Lexington, Massachusetts, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Duffy
authors.affiliation: Department of Gastroenterology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.affiliation: Hines VA Medical Center, Hines, and Loyola University Chicago Stritch School of Medicine, Maywood, Illinois, USA.
authors.forename: Susan P
authors.initials: SP
authors.lastname: Sambol
authors.affiliation: Hines VA Medical Center, Hines, and Loyola University Chicago Stritch School of Medicine, Maywood, Illinois, USA.
authors.forename: Dale N
authors.initials: DN
authors.lastname: Gerding
authors.affiliation: Department of Gastroenterology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
authors.forename: Ciarán P
authors.initials: CP
authors.lastname: Kelly
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000378:metabolism*; D003015:Clostridium Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000737:chemistry* / Q000382:microbiology*; D006801:Humans
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C031815:toxB protein, Clostridium difficile