title: [Cytotoxicity of cytosine deaminase and herpes simplex virus thymidine kinase genes in melanoma cells is independent on promoter strength].
abstract: In preparation of the therapeutic genetic constructs aimed to the gene-programmed enzymatic transformation of the non-toxic prodrug into toxin within cancer cells the right choice of regulatory elements (promoters and enhancers) is essential. This is widely accepted that the efficiency of the gene therapy constructions is dependent, in particular, on the strength of promoters driving the expression of the therapeutic genes. In this work we demonstrated, using the melanoma-specific promoters and enhancers of human melanoma inhibitory activity and mouse tyrosinase gene, that for the development of cytotoxic effect the promoter strength is not of primary importance. In the case of HSVtk, coding for the herpes simplex virus thymidine kinase, and FCU1, coding for cytosine deaminase/uracil phosphoribosyltransferase hybrid protein genes, their cytotoxic activity was determined by the quantity of the added prodrug.
authors.forename: I V
authors.initials: IV
authors.lastname: Alekseenko
authors.forename: D V
authors.initials: DV
authors.lastname: Kuz'min
authors.forename: V V
authors.initials: VV
authors.lastname: Pleshkan
authors.forename: M V
authors.initials: MV
authors.lastname: Zinov'eva
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000818:Animals; D043525:Cytosine Deaminase / Q000235:genetics* / Q000627:therapeutic use; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D006801:Humans; D008546:Melanoma, Experimental / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D051379:Mice; D010430:Pentosyltransferases / Q000235:genetics* / Q000627:therapeutic use; D011355:Prodrugs / Q000627:therapeutic use; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics
chemical_list: D011355:Prodrugs; D010430:Pentosyltransferases; C023269:uracil phosphoribosyltransferase; D043525:Cytosine Deaminase