title: Microbial synthesis of plant oxylipins from γ-linolenic acid through designed biotransformation pathways.
abstract: Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxylipins--a family of oxygenated unsaturated carboxylic acids--was explored to enable a high-yield production through a designed microbial synthetic system harboring a set of microbial enzymes (i.e., fatty acid double-bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases, and esterases) to produce a variety of unsaturated carboxylic acids from γ-linolenic acid. The whole cell system of the recombinant Escherichia coli efficiently produced (6Z,9Z)-12-hydroxydodeca-6,9-dienoic acid (7), (Z)-9-hydroxynon-6-enoic acid (15), (Z)-dec-4-enedioic acid (17), and (6Z,9Z)-13-hydroxyoctadeca-6,9-dienoic acid (2). This study demonstrated that various secondary metabolites of plants can be produced by implementing artificial biosynthetic pathways into whole-cell biocatalysis.
authors.forename: Sae-Um
authors.initials: SU
authors.lastname: Kim
authors.affiliation: §Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Kyoung-Rok
authors.initials: KR
authors.lastname: Kim
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Soomin
authors.initials: S
authors.lastname: Kim
authors.forename: Yong-Uk
authors.initials: YU
authors.lastname: Kwon
authors.affiliation: §Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
authors.forename: Jin-Byung
authors.initials: JB
authors.lastname: Park
mesh_terms: D053898:Biosynthetic Pathways; D001711:Biotransformation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D054883:Oxylipins / Q000378:metabolism*; D017965:gamma-Linolenic Acid / Q000378:metabolism*
chemical_list: D054883:Oxylipins; D017965:gamma-Linolenic Acid
keywords: Escherichia coli; biotransformation; plant oxylipins; whole-cell biocatalysis; γ-linolenic acid