title: Fabrication and In Vitro/In Vivo Performance of Mucoadhesive Electrospun Nanofiber Mats Containing α-Mangostin.
abstract: This study aimed to fabricate mucoadhesive electrospun nanofiber mats containing α-mangostin for the maintenance of oral hygiene and reduction of the bacterial growth that causes dental caries. Synthesized thiolated chitosan (CS-SH) blended with polyvinyl alcohol (PVA) was selected as the mucoadhesive polymer. α-Mangostin was incorporated into the CS-SH/PVA solution and electrospun to obtain nanofiber mats. Scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, and tensile strength testing were used to characterize the mats. The swelling degree and mucoadhesion were also determined. The nanofiber mats were further evaluated regarding their α-mangostin content, in vitro α-mangostin release, antibacterial activity, cytotoxicity, in vivo performance, and stability. The results indicated that the mats were in the nanometer range. The α-mangostin was well incorporated into the mats, with an amorphous form. The mats showed suitable tensile strength, swelling, and mucoadhesive properties. The loading capacity increased when the initial amount of α-mangostin was increased. Rapid release of α-mangostin from the mats was achieved. Additionally, a fast bacterial killing rate occurred at the lowest concentration of nanofiber mats when α-mangostin was added to the mats. The mats were less cytotoxic after use for 72 h. Moreover, in vivo testing indicated that the mats could reduce the number of oral bacteria, with a good mouth feel. The mats maintained the amount of α-mangostin for 6 months. The results suggest that α-mangostin-loaded mucoadhesive electrospun nanofiber mats may be a promising material for oral care and the prevention of dental caries.
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Wipada
authors.initials: W
authors.lastname: Samprasit
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Akkaramongkolporn
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
authors.affiliation: Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand. ruchadapornk@yahoo.com.
authors.forename: Ruchadaporn
authors.initials: R
authors.lastname: Kaomongkolgit
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand. opanasopit_p@su.ac.th.
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
mesh_terms: D000268:Adhesiveness; D000278:Administration, Buccal; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009061:Mouth Mucosa / Q000378:metabolism*; D057139:Nanofibers; D036103:Nanotechnology / Q000379:methods; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D012995:Solubility; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D013298:Streptococcus sanguis / Q000187:drug effects / Q000254:growth & development; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D013552:Swine; D013718:Tensile Strength; D014961:X-Ray Diffraction; D044004:Xanthones / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D013438:Sulfhydryl Compounds; D044004:Xanthones; D011142:Polyvinyl Alcohol; D048271:Chitosan; C021053:mangostin
keywords: dental caries; mucoadhesive property; nanofibers; thiolated chitosan; α-mangostin