title: Diagnosis and sequence analysis of avian leukosis virus subgroup J isolated from Chinese Partridge Shank chickens.
abstract: The diagnosis of avian leukosis virus subgroup J (ALV-J) infection in Chinese Partridge Shank chickens was confirmed by necropsy, histopathological examinations, antibody tests, viral isolation, immunofluorescence assays, and sequence analysis. Myelocytoma, myeloma, and fibrosarcoma were simultaneously found in Partridge Shank flock with ALV-J infection. Sequence analysis of the env genes of ALV-J demonstrated that both gp85 and gp37 were highly homologous among the three strains from local chickens of those among ALV-J strains isolated from white meat-type chickens. The phylogenetic trees indicated that the three strains isolated in this study were closely related to reference strains isolated in so-called Chinese yellow chickens and some strains isolated from white meat-type chickens, both from the USA and China. The observed ALV-J infection was the first report on Partridge Shank chickens, and myelocytoma, myeloma, and fibrosarcoma were found at the same time in this batch of local chickens.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Sidi
authors.initials: S
authors.lastname: Ju
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Fanfeng
authors.initials: F
authors.lastname: Meng
authors.affiliation: Qingdao Entry-Exit Inspection And Quarantine Bureau, Qingdao 266001, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China zzcui@sdau.edu.cn.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D002681:China / Q000453:epidemiology; D005354:Fibrosarcoma / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D015994:Incidence; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity
keywords: Avian leukosis virus subgroup J; Partridge Shank chickens; comprehensive diagnosis; sequence analysis