title: [Genetically predetermined limitation in the use of HaCaT cells that affects their ability to serve as an experimental model of psoriasis].
abstract: Proinflammatory cytokines TNF, IFNG and ILl7 play an important role in eruption of psoriasis. The activation of epidermal keratinocytes with the named cytokines alters their terminal differentiation program and causes their hyperproliferation in the diseased skin. HaCaT cells, which are immortalized human keratinocytes, are often used as a cellular model of psoriasis. The aim of this study was to evaluate changes in gene expression and the proliferation rates in cultured HaCaT cells treated with TNF, IFNG and IL17. We found that HaCaT cells decrease their proliferation rate in response to either IL17 or a combination TNF and IF-NG. The analysis of microarray data discovered a group of 12 genes, which were downregulated in HaCaT after treatments with the named cytokines and upregulated in psoriatic lesional skin. Eight genes were important for DNA replication and they also contributed to two larger networks that regulated cell progression through the cell cycle. We conclude that HaCaT cells have a sufficient limitation as a cellular model of psoriasis due to their treatment with proinflammatory cytokines, namely TNF, IFNG and IL17 does not increase their proliferation rate. Thus, the studies of psoriasis based on HaCaT cells as an experimental model shall take in account this important phenomenon.
authors.forename: A G
authors.initials: AG
authors.lastname: Soboleva
authors.forename: A D
authors.initials: AD
authors.lastname: Zolotarenko
authors.forename: V V
authors.initials: VV
authors.lastname: Sobolev
authors.forename: S A
authors.initials: SA
authors.lastname: Bruskin
authors.forename: E S
authors.initials: ES
authors.lastname: Piruzian
authors.forename: A V
authors.initials: AV
authors.lastname: Mezentsev
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism* / Q000502:physiology; D061251:Primary Cell Culture / Q000379:methods; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C573415:IFNG protein, human; D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma