title: Hericium erinaceus polysaccharide-protein HEG-5 inhibits SGC-7901 cell growth via cell cycle arrest and apoptosis.
abstract: HEG-5 is a novel polysaccharide-protein purified from the fermented mycelia of Hericium erinaceus CZ-2. The present study aims to investigate the effects of HEG-5 on proliferation, cell cycle and apoptosis of human gastric cancer cells SGC-7901. Here, we first uncover that HEG-5 significantly inhibited the proliferation and colony formation of SGC-7901 cells by promoting apoptosis and cell cycle arrest at S phase. RT-PCR and Western blot analysis suggested that HEG-5 could decrease the expressions of Bcl2, PI3K and AKT1, while increase the expressions of Caspase-8, Caspase-3, p53, CDK4, Bax and Bad. These findings indicated that the Caspase-8/-3-dependent, p53-dependent mitochondrial-mediated and PI3k/Akt signaling pathways involved in the molecular events of HEG-5 induced apoptosis and cell cycle arrest. Thus, our study provides in vitro evidence that HEG-5 may be taken as a potential candidate for treating gastric cancer.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, P.R. China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zan
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China. Electronic address: fengjiecui@163.com.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Cui
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China. Electronic address: yangyan@saas.sh.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Institute of Quality and Standard for Agroproducts, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China. Electronic address: lfping2005@gmail.com.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Ping
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001487:Basidiomycota / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D045424:Complex Mixtures / Q000737:chemistry* / Q000494:pharmacology*; D062610:Fungal Polysaccharides / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans
chemical_list: D045424:Complex Mixtures; D062610:Fungal Polysaccharides; D005656:Fungal Proteins
keywords: Anti-tumor activity; Apoptosis; Cell cycle; Hericium erinaceus; Polysaccharide-protein