title: Silver nanoparticles mediated altered gene expression of melanin biosynthesis genes in Bipolaris sorokiniana.
abstract: Melanin production in many fungal phytopathogens has been investigated to play direct or indirect role in pathogenesis. However, in Bipolaris sorokiniana, the spot blotch pathogen of wheat, much less is known about the role melanin play in pathogenesis. As an extension of our previous report, the present study aims to investigate the plausible association between melanin production and virulence factor in B. sorokiniana. In the previous study, we carried out analysis on the antifungal efficacy of biosynthesized silver nanoparticles (AgNPs) against B. sorokiniana. The present investigation revealed the gene expression analysis of melanin biosynthesis genes viz. polyketide synthase (PKS1) and scytalone dehydratase (SCD1) under the influence of AgNPs. The 0.05mg/ml concentration of AgNPs yielded noticeable inhibition of B. sorokiniana growth, while 0.1mg/ml concentration of AgNPs accounted for complete inhibition of pathogen growth. In addition, the semiquantitative RT-PCR analysis exhibited reduced expression of PKS1 and SCD1 under the influence of AgNPs treatment. Furthermore, the qRT-PCR demonstrated 6.47 and 1.808 fold significant decrease in the expression pattern of PKS1 and SCD1, respectively, in B. sorokiniana treated with AgNPs. The present study provides probable understanding of molecular events underlying the antifungal role of AgNPs against B. sorokiniana.
authors.affiliation: Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi221005, India.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi221005, India. Electronic address: hbs1@rediffmail.com.
authors.forename: H B
authors.initials: HB
authors.lastname: Singh
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D001203:Ascomycota / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005796:Genes; D006836:Hydro-Lyases / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D053758:Nanoparticles / Q000378:metabolism*; D048630:Polyketide Synthases / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012834:Silver / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D008543:Melanins; D012834:Silver; D048630:Polyketide Synthases; D006836:Hydro-Lyases; C082741:scytalone dehydratase
keywords: Bipolaris sorokiniana; Melanin; Polyketide synthase; Scytalone dehydratase; Silver nanoparticles