title: Glycoprotein-based enzyme-linked immunosorbent assays for serodiagnosis of infectious laryngotracheitis.
abstract: For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, USA.
authors.forename: Mallikarjuna
authors.initials: M
authors.lastname: Kanabagatte Basavarajappa
authors.affiliation: Zoetis, College Park, Maryland, USA.
authors.forename: Haichen
authors.initials: H
authors.lastname: Song
authors.affiliation: Zoetis, College Park, Maryland, USA.
authors.forename: Chinta
authors.initials: C
authors.lastname: Lamichhane
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, USA ssamal@umd.edu.
authors.forename: Siba K
authors.initials: SK
authors.lastname: Samal
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000276:immunology / Q000302:isolation & purification*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D012698:Serologic Tests / Q000379:methods*; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000914:Antibodies, Viral