title: Intraperitoneally administered biliverdin protects against UVB-induced skin photo-damage in hairless mice.
abstract: Oxidative stress is shown to be responsible for ultraviolet B (UVB) irradiation-induced skin cancer and premature aging. Biliverdin (BVD), a product of heme oxygenase-1, has strong anti-oxidant and anti-inflammatory properties. In the present study, we investigated the effects of BVD on UVB-induced skin photo-damage in hairless mice. Mice were divided into three groups: control group, UVB group (only UVB irradiation) and BVD+UVB group (mice were intraperitoneally injected with BVD before each UVB irradiation). Intraperitoneal BVD injection resulted in a significant photoprotective effect by reducing morphological and histopathological changes to the skin. BVD also exhibited a significant antioxidant effect by increasing the superoxide dismutase (SOD) level and decreasing the thiobarbituric acid reactive substances (TBARS) level compared with the control group. In addition, BVD activated biliverdin reductase (BVR) expression and inhibited the UVB-induced increase of p38 mitogen-activated protein kinase phosphorylation (p-p38MAPK), MMP (matrix metalloproteinase)-1 and MMP-3 expression (p<0.05). It also significantly decreased the interleukin (IL)-6 level compared with the UVB group (p<0.05). In conclusion, these data suggest that the intraperitoneally administered BVD can prevent UVB irradiation-induced skin photo-damage in hairless mice and that this is likely mediated by its antioxidant and anti-inflammatory mechanisms and cell signal regulatory action.
authors.affiliation: The Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province 150081, China.
authors.forename: Bingxue
authors.initials: B
authors.lastname: Bai
authors.affiliation: The Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province 150081, China.
authors.forename: Yingdi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Department of Dermatology, The Third Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province 150040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: You
authors.affiliation: The Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province 150081, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province 150081, China. Electronic address: maliangjuan@163.com.
authors.forename: Liangjuan
authors.initials: L
authors.lastname: Ma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001664:Biliverdine / Q000008:administration & dosage* / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000096:biosynthesis; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase; D048051:p38 Mitogen-Activated Protein Kinases; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1; D001664:Biliverdine