title: The cytoplasm as a radiation target: an in silico study of microbeam cell irradiation.
abstract: We performed in silico microbeam cell irradiation modelling to quantitatively investigate ionisations resulting from soft x-ray and alpha particle microbeams targeting the cytoplasm of a realistic cell model. Our results on the spatial distribution of ionisations show that as x-rays are susceptible to scatter within a cell that can lead to ionisations in the nucleus, soft x-ray microbeams may not be suitable for investigating the DNA damage response to radiation targeting the cytoplasm alone. In contrast, ionisations from an ideal alpha microbeam are tightly confined to the cytoplasm, but a realistic alpha microbeam degrades upon interaction with components upstream of the cellular target. Thus it is difficult to completely rule out a contribution from alpha particle hits to the nucleus when investigating DNA damage response to cytoplasmic irradiation. We find that although the cytoplasm targeting efficiency of an alpha microbeam is better than that of a soft x-ray microbeam (the probability of stray alphas hitting the nucleus is 0.2% compared to 3.6% for x-rays), stray alphas produce more ionisations in the nucleus and thus have greater potential for initiating damage responses therein. Our results suggest that observed biological responses to cytoplasmic irradiation include a small component that can be attributed to stray ionisations in the nucleus resulting from the stochastic nature of particle interactions that cause out-of-beam scatter. This contribution is difficult to isolate experimentally, thus demonstrating the value of the in silico approach.
authors.affiliation: Institute of Medical Physics, School of Physics, University of Sydney, NSW 2006, Australia.
authors.forename: H L
authors.initials: HL
authors.lastname: Byrne
authors.forename: W
authors.initials: W
authors.lastname: Domanova
authors.forename: A L
authors.initials: AL
authors.lastname: McNamara
authors.forename: S
authors.initials: S
authors.lastname: Incerti
authors.forename: Z
authors.initials: Z
authors.lastname: Kuncic
mesh_terms: D000512:Alpha Particles; D002467:Cell Nucleus / Q000528:radiation effects; D003198:Computer Simulation; D003593:Cytoplasm / Q000528:radiation effects*; D014965:X-Rays