title: Chemical composition, antibacterial and anticancer activities of volatile oil of Melicope denhamii leaves.
abstract: Melicope denhamii leaf volatile oil was isolated by hydrodistillation, and twenty-six constituents comprising 95.95% of the leaf oil were characterised by gas chromatographic techniques. Sesquiterpenes, zierone (22.49%) and α-gurjunene (19.96%), were identified as the major components. M. denhamii leaf oil tested against Gram-positive and Gram-negative bacteria showed significant activity against Bacillus subtilis and Escherichia coli. Anticancer activity of M. denhamii leaf oil against Dalton's lymphoma ascites cells was assessed by trypan blue exclusion and MTT assays, and the oil showed significant cytotoxicity at CD50 of 12.2 μg/mL. Induction of apoptosis on DLA cells by M. denhamii leaf oil was confirmed by morphological observation, nuclear damage and comet assays.
authors.affiliation: a Phytochemistry and Phytopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute , Pacha-Palode, Thiruvananthapuram 695 562 , Kerala , India.
authors.forename: Sony
authors.initials: S
authors.lastname: George
authors.forename: S Ajikumaran
authors.initials: SA
authors.lastname: Nair
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Venkataraman
authors.forename: Sabulal
authors.initials: S
authors.lastname: Baby
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D017209:Apoptosis; D045744:Cell Line, Tumor; D051379:Mice; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D027821:Rutaceae / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes
keywords: MTT assay; Melicope denhamii; antibacterial; anticancer; leaf oil