title: Structural characterization and antioxidant activities of κ-carrageenan oligosaccharides degraded by different methods.
abstract: In the present study, four kinds of κ-carrageenan oligosaccharides were obtained by the degradation of parent κ-carrageenan using free radical depolymerization, mild acid hydrolysis, κ-carrageenase digestion and partial reductive hydrolysis, respectively. Their structure types were accurately and comparatively elucidated by ESI-MS and CID MS/MS. The antioxidant activities of different degraded products were investigated by four different antioxidant assays, including superoxide radical scavenging activity, hydroxyl radical scavenging activity, reducing power and DPPH radical scavenging activity. The methods of depolymerization had an influence on the antioxidant activities of κ-carrageenan oligosaccharides obtained from κ-carrageenan. These results indicated that the antioxidant activities of κ-carrageenan oligosaccharides could be related to the degree of polymerization, the content of reducing sugar and sulfate groups, and the structure of reducing terminus.
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Bingying
authors.initials: B
authors.lastname: Yang
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gu
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Chengjian
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Glycoengineering Research Center, Shandong University, Jinan 250012, PR China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China. Electronic address: huanglj@nwu.edu.cn.
authors.forename: Linjuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China. Electronic address: wangzhf@nwu.edu.cn.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002351:Carrageenan / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D006868:Hydrolysis; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D009844:Oligosaccharides; D002351:Carrageenan
keywords: Antioxidant activities; Depolymerization; ESI-MS analysis; κ-Carrageenan