title: A comparative study of the effects of 4-META/MMA-TBB resin and cyanoacrylate on wound healing of skin defects.
abstract: The purpose of this study was to investigate the healing process of wounded skin following the application of cyanoacrylate or a 4-(2-methacryloxyethyl) trimellitic anhydride/methyl methacrylate-tributylborane resin (4-META resin). Those materials were applied to skin wound areas in rats, and the regenerating tissues were biopsied and examined at days 1, 3, 5, 7, and 14. Paraffin-embedded specimens were sectioned and stained with hematoxylin and eosin or with Azan-Mallory stain. Sections were also immunohistochemically stained with Pan-cytokeratin and CD68 antibodies. In cyanoacrylate-treated wounds, CD68-positive cells were observed in the connective tissue and their number increased up to day 5. The wound surface was completely covered by epithelial tissue at day 14. In 4-META resin-treated wounds, CD68-positive cells appeared in the soft-tissue hybrid layer (STHL) and epithelial tissue had migrated under the STHL by day 5. The wound surface was completely covered by epithelial tissue at day 7. CD68-positive cells were distributed over the entire area of the cyanoacrylate-treated wounds, but accumulated under the STHL in the 4-META resin-treated wounds. In conclusion, the results suggest that covering skin defects with a 4-META resin is an effective strategy to promote wound healing compared to cyanoacrylate.
authors.affiliation: Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College, Ichikawa, Chiba, 272-8513, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kidokoro
authors.affiliation: Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.|Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.|Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kobayashi
authors.affiliation: Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.|Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.|Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.affiliation: Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College, Ichikawa, Chiba, 272-8513, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Katakura
authors.affiliation: Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.|Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology*; D003487:Cyanoacrylates; D008297:Male; D008768:Methylmethacrylates / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000180:Acrylic Resins; D001896:Boron Compounds; D003487:Cyanoacrylates; D008768:Methylmethacrylates; C476987:methylmethacrylate-tributylborane resin; C031172:4-META resin
keywords: 4-META/TBB-MMA resin; cyanoacrylate; immunohistochemical staining; skin; wound healing