title: MicroRNA-424 may function as a tumor suppressor in endometrial carcinoma cells by targeting E2F7.
abstract: MicroRNAs (miRNAs) are frequently dysregulated in human cancers and can act as potent oncogenes or tumor suppressor genes. Aberrant expression of miR-424 has been identified in some types of cancer, however, its expression and potential biologic role in endometrial cancer are remains to be determined. In the present study, we demonstrated that miR-424 was downregulated in human endometrial cancer and suppressed growth of the human Ishikawa and HEC-1B endometrial cancer cell lines. Bioinformatics analysis indicated that E2F7 was a putative target of miR-424. In a luciferase reporter system, we confirmed that E2F7 was a direct target gene of miR-424. Furthermore, knockdown of E2F7 inhibited Ishikawa and HEC-1B cell growth. These findings indicate that miR-424 targets the E2F7 transcript and suppresses endometrial cancer cell growth, suggesting that miR-424 has a tumor suppressive role in human endometrial cancer pathogenesis.
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Qiu
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Qing-Han
authors.initials: QH
authors.lastname: Li
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of Gynaecology, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizou 563000, P.R. China.
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: Xiao
mesh_terms: D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D050702:E2F7 Transcription Factor / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050702:E2F7 Transcription Factor; C481054:E2F7 protein, human; C554214:MIRN424 microrna, human; D035683:MicroRNAs; D012333:RNA, Messenger