title: Characterization of Tn6238 with a new allele of aac(6')-Ib-cr.
abstract: Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6')-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6')-Ib-like gene cassettes suggests that this new aac(6')-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants.
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María P
authors.initials: MP
authors.lastname: Quiroga
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Betina
authors.initials: B
authors.lastname: Orman
authors.affiliation: Sección Microbiología, Hospital Fernández, Buenos Aires, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Errecalde
authors.affiliation: Sección Microbiología, Hospital Fernández, Buenos Aires, Argentina.
authors.forename: Sara
authors.initials: S
authors.lastname: Kaufman
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina dcentron@gmail.com.
authors.forename: Daniela
authors.initials: D
authors.lastname: Centrón
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019295:Computational Biology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones