title: Rhodopsin purification from dark-adapted bovine retina.
abstract: Structural and biophysical studies of rhodopsin have long depended upon the ready availability of bovine retina from the meat-packing industry and the relative ease of obtaining homogenous preparations of rhodopsin in the quantities and purities necessary for such study. Herein we present a modular purification methodology employing a combination of several strategies, beginning with sucrose gradient isolation of rod outer segments (ROS) from bovine retina, detergent solubilization of ROS, selective extraction of rhodopsin starting from this detergent-solubilized ROS, and further purification via size-exclusion chromatography, resulting in a preparation of high-purity rhodopsin at high concentration suitable for crystallization or other biophysical study.
authors.affiliation: Case Center for Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, 10900 Euclid Ave., Cleveland, OH, 44106-4965, USA.
authors.forename: Elise
authors.initials: E
authors.lastname: Blankenship
authors.forename: David T
authors.initials: DT
authors.lastname: Lodowski
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D008027:Light; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism
chemical_list: D012243:Rhodopsin