title: Gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p inhibit growth of EBV-positive nasopharyngeal carcinoma.
abstract: Epstein-Barr virus (EBV) infection is a major etiological factor for nasopharyngeal carcinoma (NPC). Several EBV-encoded BART miRNAs have been associated with viral latency, immune escape, cell survival, cell proliferation and apoptosis. Here, we report that EBV-miR-BART7-3p, an EBV-encoded BART miRNA highly expressed in NPC, was correlated with cell-cycle progression in vitro and increased tumor formation in vivo. This viral miRNA stimulated the PTEN/PI3K/Akt pathway and induced c-Myc and c-Jun. Knockdown of PTEN mimicked EBV-miR-BART7-3p-induced tumorigenic phenotype. Based on these results, we conducted a therapeutic experiment by using gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p. Silencing of EBV-miR-BART7-3p reduced tumor growth in animal model. We conclude that EBV-miR-BART7-3p favors carcinogenesis, representing a potential target for miRNA-based therapy.
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Longmei
authors.initials: L
authors.lastname: Cai
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jinbang
authors.initials: J
authors.lastname: Li
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.|The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.|Department of Radiation Oncology, Gaozhou People's Hospital, Gaozhou 525200, China.
authors.forename: Yaoyong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.|Central Medical Laboratory, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lyu
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chinese Traditional Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jinkun
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Chinese Traditional Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Cai
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis; D002277:Carcinoma; D049109:Cell Proliferation / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000378:metabolism / Q000473:pathology / Q000628:therapy* / Q000821:virology*; D015316:Genetic Therapy / Q000379:methods; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D057809:HEK293 Cells; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy* / Q000821:virology*; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
chemical_list: D035683:MicroRNAs; D006046:Gold
keywords: EBV-miR-BART7; nasopharyngeal carcinoma; therapeutic experiment; tumorigenesis