title: FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis.
abstract: The plant vacuole is a central organelle that is involved in various biological processes throughout the plant life cycle. Elucidating the mechanism of vacuole biogenesis and maintenance is thus the basis for our understanding of these processes. Proper formation of the vacuole has been shown to depend on the intracellular membrane trafficking pathway. Although several mutants with altered vacuole morphology have been characterized in the past, the molecular basis for plant vacuole biogenesis has yet to be fully elucidated. With the aim to identify key factors that are essential for vacuole biogenesis, we performed a forward genetics screen in Arabidopsis (Arabidopsis thaliana) and isolated mutants with altered vacuole morphology. The vacuolar fusion defective1 (vfd1) mutant shows seedling lethality and defects in central vacuole formation. VFD1 encodes a Fab1, YOTB, Vac1, and EEA1 (FYVE) domain-containing protein, FYVE1, that has been implicated in intracellular trafficking. FYVE1 localizes on late endosomes and interacts with Src homology-3 domain-containing proteins. Mutants of FYVE1 are defective in ubiquitin-mediated protein degradation, vacuolar transport, and autophagy. Altogether, our results show that FYVE1 is essential for plant growth and development and place FYVE1 as a key regulator of intracellular trafficking and vacuole biogenesis.
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Cornelia
authors.initials: C
authors.lastname: Kolb
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Marie-Kristin
authors.initials: MK
authors.lastname: Nagel
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Kamila
authors.initials: K
authors.lastname: Kalinowska
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Jörg
authors.initials: J
authors.lastname: Hagmann
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Mie
authors.initials: M
authors.lastname: Ichikawa
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Franziska
authors.initials: F
authors.lastname: Anzenberger
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Angela
authors.initials: A
authors.lastname: Alkofer
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Masa H
authors.initials: MH
authors.lastname: Sato
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.).
authors.forename: Pascal
authors.initials: P
authors.lastname: Braun
authors.affiliation: Plant Systems Biology, Technische Universität München, 85354 Freising, Germany (C.K., M.-K.N., K.K., F.A., A.A., P.B., E.I.);Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tuebingen, Germany (J.H.); andDepartment of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan (M.I., M.H.S.) erika.isono@wzw.tum.de.
authors.forename: Erika
authors.initials: E
authors.lastname: Isono
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001343:Autophagy; D003593:Cytoplasm / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D017930:Genes, Reporter; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D057149:Ubiquitinated Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000597558:FREE1 protein, Arabidopsis; D057149:Ubiquitinated Proteins; D033921:Vesicular Transport Proteins