title: The significance of grafting collagen on polycaprolactone composite scaffolds: processing-structure-functional property relationship.
abstract: The study concerns processing-structure-functional property relationship in organic-inorganic hybrid scaffolds based on grafted collagen for bone tissue engineering. Biodegradable polyester, polycaprolactone (PCL) and nanohydroxyapatite were used to fabricate three-dimensional porous scaffolds by adopting a combination of solvent casting, particulate leaching, and polymer leaching approaches. The PCL scaffold was subsequently surface modified by chemical bonding of 1,6-hexanediamine to the ester groups of PCL to introduce free NH2 groups. The introduction of NH2 groups as active sites enabled immobilization of biocompatible macromolecule, collagen, on the aminolyzed PCL via a cross-linking agent, glutaraldehyde. The osteoblasts' functions, notably cell adhesion, proliferation, and mineralization, were favorably modulated because of the chemical interaction between Arg-Gly-Asp domains in collagen molecule and integrin receptor in the cell membrane. The study underscores the significance of grafting collagen on PCL-nHA scaffold in modulating cellular activity and biological functions expanding its current use in soft tissue engineering to hard tissue regeneration.
authors.affiliation: Biomaterials and Macromolecular Science Laboratory, Center for Structural and Functional Materials Research and Innovation, and Department of Metallurgical and Materials Engineering, University of Texas at El Paso, 500 W., University Avenue, El Paso, TX, 79968, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kiran
authors.affiliation: Biomaterials and Macromolecular Science Laboratory, Center for Structural and Functional Materials Research and Innovation, and Department of Metallurgical and Materials Engineering, University of Texas at El Paso, 500 W., University Avenue, El Paso, TX, 79968, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Nune
authors.affiliation: Biomaterials and Macromolecular Science Laboratory, Center for Structural and Functional Materials Research and Innovation, and Department of Metallurgical and Materials Engineering, University of Texas at El Paso, 500 W., University Avenue, El Paso, TX, 79968, USA.
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D016475:3T3 Cells; D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002113:Calcification, Physiologic; D003094:Collagen / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: collagen; osteoblasts; polycaprolactone; scaffold