title: Thermo-chemotherapy Induced miR-218 upregulation inhibits the invasion of gastric cancer via targeting Gli2 and E-cadherin.
abstract: Thermo-chemotherapy has been proven to reduce the invasion capability of cancer cells. However, the molecular mechanism underlying this anti-invasion effect is still unclear. In this study, the role of thermo-chemotherapy in the inhibition of tumor invasion was studied. The results demonstrated that expression of miR-218 was downregulated in gastric cancer tissues, which had a positive correlation with tumor invasion and metastasis. In vitro thermo-chemotherapy increased miR-218 expression in SGC7901 cells and inhibited both proliferation and invasion of cancer cells. Gli2 was identified as a downstream target of miR-218, and its expression was negatively regulated by miR-218. The thermo-chemotherapy induced miR-218 upregulation was also accompanied by increasing of E-cadherin expression. In conclusion, the present study indicates that thermo-chemotherapy can effectively decrease the invasion capability of cancer cells and increase cell-cell adhesion. miR-218 and its downstream target Gli2, as well as E-cadherin, participate in the anti-invasion process.
authors.affiliation: Department of Hepatobiliary Surgery, Cancer Institute and Hospital, Guangzhou Medical University, Guangzhou, 510095, Guangdong, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Fang
authors.forename: Shu-Zhong
authors.initials: SZ
authors.lastname: Cui
authors.forename: Xiang-Liang
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yin-Bing
authors.initials: YB
authors.lastname: Wu
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Tang
authors.forename: Yi-Nuo
authors.initials: YN
authors.lastname: Tu
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D006979:Hyperthermia, Induced; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D000075746:Zinc Finger Protein Gli2
chemical_list: D015820:Cadherins; C082593:GLI2 protein, human; D051741:Kruppel-Like Transcription Factors; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D000075746:Zinc Finger Protein Gli2