title: Identification of potential protein markers of noble rot infected grapes.
abstract: The evaluation of Botrytis cinerea as noble rot on withered grapes is of great importance to predict the wine sensory/organoleptic properties and to manage the winemaking process of Amarone, a passito dry red wine. This report describes the first proteomic analysis of grapes infected by noble rot under withering conditions to identify possible markers of fungal infection. 2-D gel electrophoresis revealed that protein profiles of infected and not infected grape samples are significantly different in terms of number of spots and relative abundance. Protein identification by MS analysis allowed to identify only in infected berries proteins of B. cinerea that represent potential markers of the presence of the fungus in the withered grapes.
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: Marilinda
authors.initials: M
authors.lastname: Lorenzini
authors.affiliation: Department of Medicine, University of Padova, Via Giustiniani 2, 35128 Padova, Italy; Proteomics Center of Padova University, Via G. Orus 2/B, 35129 Padova, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Millioni
authors.affiliation: Proteomics Center of Padova University, Via G. Orus 2/B, 35129 Padova, Italy; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Franchin
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Zapparoli
authors.affiliation: Proteomics Center of Padova University, Via G. Orus 2/B, 35129 Padova, Italy; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy. Electronic address: giorgio.arrigoni@unipd.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Arrigoni
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy. Electronic address: barbara.simonato@univr.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Simonato
mesh_terms: D015415:Biomarkers; D015180:Electrophoresis, Gel, Two-Dimensional; D005658:Fungi / Q000302:isolation & purification*; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics; D027843:Vitis / Q000737:chemistry* / Q000382:microbiology
chemical_list: D015415:Biomarkers; D010940:Plant Proteins
keywords: 2-DE; Amarone wine; Botrytis cinerea; Grape proteins; Mass spectrometry; Proteomics