title: Development of a loop-mediated isothermal amplification assay for rapid detection of Trichosporon asahii in experimental and clinical samples.
abstract: Invasive trichosporonosis is a deep mycosis found mainly in immunocompromised hosts, and the major pathogen is Trichosporon asahii. We detected the species-specific intergenic spacers (IGS) of rRNA gene of T. asahii using a loop-mediated isothermal amplification (LAMP) assay in 15 isolates with 3 different visualization methods, including SYBR green detection, gel electrophoresis, and turbidimetric methods. The LAMP assay displayed superior rapidity to other traditional methods in the detection time; that is, only 1 h was needed for detection and identification of the pathogen DNA. Furthermore, the detection limit of the LAMP assay was more sensitive than the PCR assay. We also successfully detect the presence of T. asahii in samples from experimentally infected mice and samples from patients with invasive trichosporonosis caused by T. asahii, suggesting that this method may become useful in clinical applications in the near future.
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China ; The Clinical Medical College in the Beijing Military Region of Second Military Medical University of PLA, Beijing 100700, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China.
authors.forename: Rongya
authors.initials: R
authors.lastname: Yang
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009181:Mycoses / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014250:Trichosporon / Q000235:genetics*; D060586:Trichosporonosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal