title: Next-generation sequencing identified microRNAs that associate with motile aeromonad septicemia in grass carp.
abstract: MicroRNAs (miRNAs) modulate many biological processes and can be up-or down-regulated in a variety of diseases states, including in bacterial infection. In this study, we characterized miRNAs associated with susceptibility or resistance to motile Aeromonas hydrophila in grass carp (Ctenopharyngodon idella), a commercially important farmed fish species in China. Using Illumina next-generation sequencing, we detected 185 miRNAs in A. hydrophila-susceptible (SGC) and -resistant grass carp (RGC) during immune activation. Twenty-one miRNAs were found to be differentially expressed between SGC and RGC, with 23.8% (5 out 21) exhibiting elevated expression in SGC. Further investigation of a selected five miRNAs indicated differences in the timing and tissue sources of expression. Target genes likely regulated by these miRNAs were identified using computational prediction combined with transcriptome expression data. MiRNA targets of differentially expressed mRNAs included those with functions important for bacterial resistance, such as tlr4 and nfil3-6. In addition, we demonstrated that nfil3-6 and tlr4 genes are direct targets of let-7i and cid-miRn-118. The present study suggested that the multiple miRNAs, displaying diverse kinetics and tissue sources on antibacterial immune processes.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, PR China.
authors.forename: Yubang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Fu
authors.affiliation: National Pathogen Collection Center for Aquatic Animals, College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Lu
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, PR China; E-Institute of Shanghai Universities, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, PR China. Electronic address: jlli2009@126.com.
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology*; D000818:Animals; D002347:Carps; D004198:Disease Susceptibility / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018805:Sepsis / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Aeromonas hydrophila; Ctenopharyngodon idella; Motile aeromonad septicemia; let-7i; microRNA