title: Survival of Lactobacillus casei strain Shirota in the intestines of healthy Chinese adults.
abstract: Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of a probiotic beverage containing 1.0 × 10(8) CFU/mL of LcS for 14 days. LcS were enumerated using a culture method and the colony identity confirmed by ELISA. Fourteen days after ingestion, the amount of LcS recovered from fecal samples was between 6.86 ± 0.80 and 7.17 ± 0.57 Log10 CFU/g of feces (mean ± SD). The intestinal microbiotas were analyzed by denaturing gradient gel electrophoresis. Principal component analysis showed that consuming LcS significantly changed fecal microbiota profiles. According to redundancy analysis, the amounts of 25 bacterial strains were significantly correlated with LcS intake (P < 0.05), 11 of them positively and fourteen negatively. Concentrations of acetic acid and propionic acid in feces were significantly lower during the ingestion period than during the baseline period (P < 0.05). These results confirm that LcS can survive passage through the gastrointestinal tract of Chinese people; however, they were found to have little ability to persist once their consumption had ceased. Furthermore, consumption of probiotic beverages containing LcS can modulate the composition of the intestinal microbiota on a long-term basis, resulting in decreased concentrations of SCFAs in the gut.
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083.
authors.forename: Shanbin
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Food Science and Engineering, Beijing University of Agriculture, Beijing, 102206.
authors.forename: Junhua
authors.initials: J
authors.lastname: Jin
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083.
authors.forename: Fazheng
authors.initials: F
authors.lastname: Ren
authors.affiliation: Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100083, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100083, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100083, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D000328:Adult; D044466:Asian People; D058448:Biota; D015169:Colony Count, Microbial; D058645:Denaturing Gradient Gel Electrophoresis; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007780:Lacticaseibacillus casei / Q000502:physiology*; D008297:Male; D050296:Microbial Viability; D019936:Probiotics / Q000008:administration & dosage*; D011422:Propionates / Q000032:analysis; D055815:Young Adult
chemical_list: D011422:Propionates; C029658:propionic acid; D019342:Acetic Acid
keywords: Chinese adults; intestinal microbiota; recovery of  Lactobacillus casei strain Shirota; short-chain fatty acids