title: Methods in molecular cardiology: in silico cloning.
abstract: Advancements in sequencing technology have made it possible to obtain more information about the DNA sequence, structure and the transcript products of the genome from different species. This information is collected in DNA databases. These databases contain many genes of which the functions have not yet been discovered. By using online biotechnology tools novel genes and their transcripts can be identified. The identification of novel genes using DNA database analysis is referred to as in silico cloning. In silico cloning may not only provide new information on genes and their biological function, it may also lead to identification of molecular targets for drug discovery activities. In this review we describe the process of in silico cloning and its application in biomedical research.
authors.forename: R
authors.initials: R
authors.lastname: Passier
authors.forename: P A
authors.initials: PA
authors.lastname: Doevendans
keywords: DNA sequences; silico cloning