title: Highly stable and sensitive fluorescent probes (LysoProbes) for lysosomal labeling and tracking.
abstract: We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Nazmiye B
authors.initials: NB
authors.lastname: Yapici
authors.affiliation: Second Hospital of HeBei Medical University, Shijiazhuang, China 050000.
authors.forename: Yue
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Second Hospital of HeBei Medical University, Shijiazhuang, China 050000.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Srinivas Rao
authors.initials: SR
authors.lastname: Mandalapu
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Megan
authors.initials: M
authors.lastname: Faucett
authors.affiliation: Department of Chemistry, Columbia University, NY 10027.
authors.forename: Steffen
authors.initials: S
authors.lastname: Jockusch
authors.affiliation: Translational Research Laboratory, Stony Brook Medicine, Stony Brook, NY 11794.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Ju
authors.affiliation: Experimental and Systems Pharmacology, College of Pharmacy, Washington State University, Spokane WA 99202.
authors.forename: K Michael
authors.initials: KM
authors.lastname: Gibson
authors.affiliation: National Human Genome Research Institute, NIH, Bethesda, Maryland 20892.
authors.forename: William J
authors.initials: WJ
authors.lastname: Pavan
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Lanrong
authors.initials: L
authors.lastname: Bi
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000737:chemistry; D008247:Lysosomes / Q000737:chemistry* / Q000502:physiology; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D007785:Lactose