title: Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis.
abstract: Accumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modifications in Arabidopsis cells during the early response to salicylate or flg22, two defense pathway elicitors that are known to disturb cellular redox homeostasis. Among the salicylate- and/or flg22-responsive redox-sensitive proteins are those involved in transcriptional regulation, chromatin remodeling, RNA processing, post-translational modifications, and nucleocytoplasmic shuttling. The identification of the salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from which further study can be conducted toward understanding biological significance of their oxidative modifications during the plant defense response.
authors.affiliation: 1] Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong [2] Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Biosciences Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Huoming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong.
authors.forename: Boying
authors.initials: B
authors.lastname: Yu
authors.affiliation: Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Liming
authors.initials: L
authors.lastname: Xiong
authors.affiliation: 1] Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong [2] Partner State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Yiji
authors.initials: Y
authors.lastname: Xia
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005408:Flagellin / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D012459:Salicylates; D005408:Flagellin