title: Development and GBS-genotyping of introgression lines (ILs) using two wild species of rice,O. meridionalisandO. rufipogon,in a common recurrent parent,O.sativacv. Curinga.
abstract: Two populations of interspecific introgression lines (ILs) in a common recurrent parent were developed for use in pre-breeding and QTL mapping. The ILs were derived from crosses between cv Curinga, atropical japonicaupland cultivar, and two different wild donors,Oryza meridionalisNg. accession (W2112) andOryza rufipogonGriff. accession (IRGC 105491). The lines were genotyped using genotyping-by-sequencing (GBS) and SSRs. The 32 Curinga/O. meridionalisILs contain 76.73 % of the donor genome in individual introgressed segments, and each line has an average of 94.9 % recurrent parent genome. The 48 Curinga/O. rufipogonILs collectively contain 97.6 % of the donor genome with an average of 89.9 % recurrent parent genome per line. To confirm that these populations were segregating for traits of interest, they were phenotyped for pericarp color in the greenhouse and for four agronomic traits-days to flowering, plant height, number of tillers, and number of panicles-in an upland field environment. Seeds from these IL libraries and the accompanying GBS datasets are publicly available and represent valuable genetic resources for exploring the genetics and breeding potential of rice wild relatives.
authors.affiliation: Department of Plant Breeding and Genetics, Cornell University, 162 Emerson Hall, Ithaca, NY 14853-1901 USA.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Arbelaez
authors.affiliation: School of Botany, The University of Melbourne, Parkville, VIC 3010 Australia.
authors.forename: Laura T
authors.initials: LT
authors.lastname: Moreno
authors.affiliation: Department of Plant Breeding and Genetics, Cornell University, 162 Emerson Hall, Ithaca, NY 14853-1901 USA.
authors.forename: Namrata
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Agronomy, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, 106 Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Tung
authors.affiliation: Department of Plant Breeding and Genetics, Cornell University, 162 Emerson Hall, Ithaca, NY 14853-1901 USA.
authors.forename: Lyza G
authors.initials: LG
authors.lastname: Maron
authors.affiliation: Rice Program, International Center for Tropical Agriculture (CIAT), AA6713 Cali, Colombia.
authors.forename: Yolima
authors.initials: Y
authors.lastname: Ospina
authors.affiliation: Rice Program, International Center for Tropical Agriculture (CIAT), AA6713 Cali, Colombia.
authors.forename: César P
authors.initials: CP
authors.lastname: Martinez
authors.affiliation: CIRAD-AGAP 34394, Montpellier Cedex 5, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Grenier
authors.affiliation: DIADE Research Unit, Institut de Recherche Pour le Développement, 34394 Montpellier Cedex 5, France ; Rice Genetics and Genomics Laboratory, International Center for Tropical Agriculture (CIAT), AA6713 Cali, Colombia.
authors.forename: Mathias
authors.initials: M
authors.lastname: Lorieux
authors.affiliation: Department of Plant Breeding and Genetics, Cornell University, 162 Emerson Hall, Ithaca, NY 14853-1901 USA.
authors.forename: Susan
authors.initials: S
authors.lastname: McCouch
keywords: Allele discovery; Chromosome segment substitution lines (CSSLs); Crop wild relatives; Genotyping by sequencing (GBS); Marker-assisted selection (MAS); Oryza sativa; Rice