title: Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments.
abstract: The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms.
authors.affiliation: CEA, Division of Immuno-Virology, IDMIT Center, Institute for Emerging Diseases and Innovative Therapies (iMETI), DSV, Fontenay-aux-Roses, France; Université Paris-Sud, UMR E1, Orsay, France; Vaccine Research Institute (VRI), Créteil, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Tricot
authors.forename: Mickael
authors.initials: M
authors.lastname: Meyrand
authors.forename: Chiara
authors.initials: C
authors.lastname: Sammicheli
authors.forename: Jamila
authors.initials: J
authors.lastname: Elhmouzi-Younes
authors.forename: Aurélien
authors.initials: A
authors.lastname: Corneau
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bertholet
authors.forename: Marie
authors.initials: M
authors.lastname: Malissen
authors.forename: Roger
authors.initials: R
authors.lastname: Le Grand
authors.forename: Sandra
authors.initials: S
authors.lastname: Nuti
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Antonio
authors.initials: A
authors.lastname: Cosma
mesh_terms: D000465:Algorithms; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D007554:Isotopes / Q000378:metabolism; D028581:Lanthanoid Series Elements / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D007554:Isotopes; D028581:Lanthanoid Series Elements
keywords: CyTOF; Key terms: mass cytometry; flow cytometry; standardization