title: Characterization of the UGA-recoding and SECIS-binding activities of SECIS-binding protein 2.
abstract: Selenium, a micronutrient, is primarily incorporated into human physiology as selenocysteine (Sec). The 25 Sec-containing proteins in humans are known as selenoproteins. Their synthesis depends on the translational recoding of the UGA stop codon to allow Sec insertion. This requires a stem-loop structure in the 3' untranslated region of eukaryotic mRNAs known as the Selenocysteine Insertion Sequence (SECIS). The SECIS is recognized by SECIS-binding protein 2 (SBP2) and this RNA:protein interaction is essential for UGA recoding to occur. Genetic mutations cause SBP2 deficiency in humans, resulting in a broad set of symptoms due to differential effects on individual selenoproteins. Progress on understanding the different phenotypes requires developing robust tools to investigate SBP2 structure and function. In this study we demonstrate that SBP2 protein produced by in vitro translation discriminates among SECIS elements in a competitive UGA recoding assay and has a much higher specific activity than bacterially expressed protein. We also show that a purified recombinant protein encompassing amino acids 517-777 of SBP2 binds to SECIS elements with high affinity and selectivity. The affinity of the SBP2:SECIS interaction correlated with the ability of a SECIS to compete for UGA recoding activity in vitro. The identification of a 250 amino acid sequence that mediates specific, selective SECIS-binding will facilitate future structural studies of the SBP2:SECIS complex. Finally, we identify an evolutionarily conserved core cysteine signature in SBP2 sequences from the vertebrate lineage. Mutation of multiple, but not single, cysteines impaired SECIS-binding but did not affect protein localization in cells.
authors.affiliation: a Department of Cellular and Molecular Medicine; Lerner Research Institute; Cleveland Clinic ; Cleveland , OH USA.
authors.forename: Jodi L
authors.initials: JL
authors.lastname: Bubenik
authors.forename: Angela C
authors.initials: AC
authors.lastname: Miniard
authors.forename: Donna M
authors.initials: DM
authors.lastname: Driscoll
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D018388:Codon, Terminator / Q000235:genetics*; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D017279:Selenocysteine / Q000235:genetics* / Q000378:metabolism; D051140:Selenoproteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D020413:3' Untranslated Regions; D018388:Codon, Terminator; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C498488:Secisbp2 protein, rat; D051140:Selenoproteins; D017279:Selenocysteine; D003545:Cysteine
keywords: DTT, dithiothreitol; Dio1, deiodinase 1; Dio2, deiodinase 2; GPx1, glutathione peroxidase 1; PHGPx, phospholipid hydroperoxide glutathione peroxidase; REMSA, RNA electrophoretic mobility shift assay; RNA-protein interactions; RRL, rabbit reticulocyte lysate; SBP2, SECIS binding protein 2; SECIS, Selenocysteine Insertion Sequence; SECIS-binding protein 2; Sec, selenocysteine; selenium; selenocysteine; selenoprotein; translation