title: Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections.
abstract: Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific oysters.
authors.affiliation: a Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer); Laboratoire de Génétique et Pathologie des Mollusques Marins; Ronce Les Bains ; La Tremblade , France.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Moreau
authors.forename: Kevin
authors.initials: K
authors.lastname: Moreau
authors.forename: Amélie
authors.initials: A
authors.lastname: Segarra
authors.forename: Delphine
authors.initials: D
authors.lastname: Tourbiez
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Travers
authors.forename: David C
authors.initials: DC
authors.lastname: Rubinsztein
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D001343:Autophagy; D001424:Bacterial Infections / Q000276:immunology / Q000662:veterinary; D049896:Crassostrea / Q000276:immunology* / Q000382:microbiology* / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005786:Gene Expression Regulation; D006564:Herpesviridae / Q000472:pathogenicity*; D007113:Immunity, Innate; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D014733:Vibrio / Q000472:pathogenicity*; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary
chemical_list: D004279:DNA, Viral
keywords: ATG, autophagy-related; Atg8–PE, Atg8–phosphatidylethenolamine; Crassostrea gigas; DNA, deoxyribonucleic acid; LC3-II, cleaved, lipidated and autophagosome-associated form of LC3; MAP1LC3A/B (LC3A/B), microtubule-associated proteins 1 light chain 3 alpha/beta (mammalian orthologs of the predicted Crassostrea gigas LC3 and yeast Atg8); NH4Cl, ammonium chloride; OsHV-1; OsHV-1, Ostreid herpesvirus 1; PCR, polymerase chain reaction; Pacific oyster; Vibrio aestuarianus; autophagy; hpi, hours postinfection