title: Cyclospora cayetanensis travels in tap water on Italian trains.
abstract: Tap water samples from the toilets of an Italian national railway train were collected over a period of 10 months and tested for the presence of Cyclospora cayetanensis (C. cayetanensis) using EvaGreen® real-time polymerase chain reaction (RT-PCR) assay coupled with high resolution melting (HRM) analysis for protozoan detection and oocyst quantification. C. cayetanensis positive samples were detected in March, April, and May 2013, with the number of oocysts of 4, 5, and 11 per liter, respectively. This is the first finding of C. cayetanensis in water samples in Italy. The findings call for an improvement of hygiene and water safety by the Italian national railway company.
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, 71121 Foggia, Italy E-mail: annunziata.giangaspero@unifg.it.
authors.forename: A
authors.initials: A
authors.lastname: Giangaspero
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, 71121 Foggia, Italy E-mail: annunziata.giangaspero@unifg.it.
authors.forename: M
authors.initials: M
authors.lastname: Marangi
authors.affiliation: Food & Technology Consulting SAS, 71016 S. Severo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Arace
mesh_terms: D021744:Cyclospora / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D060766:Drinking Water / Q000032:analysis*; D007558:Italy; D033661:Oocysts; D011890:Railroads; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan; D060766:Drinking Water; D014873:Water Pollutants