title: Immune responses to a recombinant Rv0057-Rv1352 fusion protein of Mycobacterium tuberculosis.
abstract: The identification and characterization of antigens of Mycobacterium tuberculosis help in understanding the mechanisms of protective immunity and in improving diagnostic methods for TB. Rv0057 and Rv1352 proteins are new T-cell antigens, found to play roles in TB infection. In this study, a recombinant fusion protein Rv0057-Rv1352 was made and analyzed for its immunological characteristics and potential utility. It showed good immunoreactivity with serum from TB patients by western blotting. The antibody levels against Rv0057-Rv1352 were significantly higher in sera from 69 TB patients than in sera from 60 patients with non-TB respiratory diseases (P<0.001). The sensitivities of a diagnostic ELISA test based on detecting Rv0057-Rv1352 antibody (60.3%) or 38 kDa-16 kDa antibody (58.8%) were comparable to commercial rapid test B (75.4%), and significantly higher (p<0.001) than bacteriological methods (15.9%), rapid test A (20.3%), or rapid test C (29.0%). The specificities of Rv0057-Rv1352 (93.3%) or 38 kDa-16 kDa antibody tests (93.3%) were equivalent to the rapid tests A (93.3%) and C (86.7%), and significantly higher than rapid test B (63.3%, p<0.001). When 38 kDa-16 kDa was used together with Rv0057-Rv1352, the test sensitivity reached 85.5%, and its specificity remained high (86.7%). The test was as sensitive with bacterium-positive TB patients as with bacterium-negative. In an ELISPOT assay for cellular immunity, Rv0057-Rv1352 stimulated T lymphocytes to produce fewer spots secreting IFN-γ than CFP10-ESAT6 fusion protein did (p>0.05). These results suggest that Rv0057-Rv1352 has potential for the serodiagnosis of active pulmonary TB.
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research.
authors.forename: Yourong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Respiration, the 309 Hospital of Chinese PLA, Beijing 100091, P. R. China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Feng
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research.
authors.forename: Junxian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiration, the 309 Hospital of Chinese PLA, Beijing 100091, P. R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Respiration, the 309 Hospital of Chinese PLA, Beijing 100091, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Bai
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research wu-xueqiong@263.net.
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Wu
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D006801:Humans; D007109:Immunity; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000276:immunology; D014374:Tuberculin Test
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins
keywords: Antigenicity; Mycobacterium tuberculosis; Recombinant fusion protein; Rv0057; Rv1352; serodiagnosis