title: Spatiotemporal Expression of EAPP Modulates Neuronal Apoptosis and Reactive Astrogliosis After Spinal Cord Injury.
abstract: E2F-associated phosphoprotein (EAPP) is a novel E2F binding protein that interacts with the activating members of the E2F transcription factors family and involved in various biological processes. However, the expression and function of EAPP in central nervous system (CNS) are still unknown. In this study, we performed an acute spinal cord injury (SCI) model in adult rats, we found that EAPP protein levels were significantly increased and reached a peak at day 3, and then gradually returned to normal level at day 14 after spinal cord injury and we observed that the expression of EAPP is enhanced in the gray and white matter. Spatially, increased levels of EAPP were striking in neurons and astrocytes. Moreover, colocalization of EAPP/active caspase-3 was detected in neurons, and colocalization of EAPP/proliferating cell nuclear antigen (PCNA) was detected in astrocytes after spinal cord injury. These results indicated that EAPP might play an important role in neuronal apoptosis and reactive astrogliosis. Furthermore in vitro, EAPP depletion by siRNA inhibited astrocyte proliferation, migration and CDK4/cyclinD1 expression. Meanwhile, EAPP knockdown also reduce neuronal apoptosis and cell cycle related proteins. Which indicated that EAPP might integrate cell cycle progression and play a crucial role in cell proliferation and apoptosis. Taken together, we speculated that EAPP was involved in biochemical and physiological responses after SCI.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Minhao
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Xishan People' Hospital, Wuxi, Jiangsu, PR China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.affiliation: Basic Medical Research Centre, Medical School, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Chengniu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D066128:Gray Matter / Q000378:metabolism / Q000473:pathology; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology*; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation; D066127:White Matter / Q000378:metabolism / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; C503750:EAPP protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D014157:Transcription Factors
keywords: CELL CYCLE; EAPP; NEURONAL APOPTOSIS; REACTIVE ASTROGLIOSIS; SPINAL CORD INJURY