title: Development and validation of an high-performance liquid chromatography-diode array detector method for the simultaneous determination of six phenolic compounds in abnormal savda munziq decoction.
abstract: OBJECTIVE
  Given the high-effectiveness and low-toxicity of abnormal savda munziq (ASMQ), its herbal formulation has long been used in traditional Uyghur medicine to treat complex diseases, such as cancer, diabetes, and cardiovascular diseases.
  
  
  METHODS
  ASMQ decoction by reversed-phase high-performance liquid chromatography coupled with a diode array detector was successfully developed for the simultaneous quality assessment of gallic acid, protocatechuic acid, caffeic acid, rutin, rosmarinic acid, and luteolin. The six phenolic compounds were separated on an Agilent TC-C18 reversed-phase analytical column (4.6 × 250 mm, 5 μm) by gradient elution using 0.3% aqueous formic acid (v/v) and 0.3% methanol formic acid (v/v) at 1.0 mL/min.
  
  
  METHODS
  The plant material was separately ground and mixed at the following ratios (10): Cordia dichotoma (10.6), Anchusa italic (10.6), Euphorbia humifusa (4.9), Adiantum capillus-veneris (4.9), Ziziphus jujube (4.9), Glycyrrhiza uralensis (7.1), Foeniculum vulgare (4.9), Lavandula angustifolia (4.9), Dracocephalum moldavica L. (4.9), and Alhagi pseudoalhagi (42.3).
  
  
  METHODS
  The precisions of all six compounds were <0.60%, and the average recoveries ranged from 99.39% to 104.85%. Highly significant linear correlations were found between component concentrations and specific chromatographic peak areas (R (2) > 0.999).
  
  
  RESULTS
  The proposed method was successfully applied to determine the levels of six active components in ASMQ.
  
  
  CONCLUSIONS
  Given the simplicity, precision, specificity, and sensitivity of the method, it can be utilized as a quality control approach to simultaneously determining the six phenolic compounds in AMSQ.
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, China.
authors.forename: Shuge
authors.initials: S
authors.lastname: Tian
authors.affiliation: Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830054, Xinjiang, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi-830054, Xinjiang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830054, Xinjiang, China.
authors.forename: Xuejia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, China ; Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830054, Xinjiang, China.
authors.forename: Halmuart
authors.initials: H
authors.lastname: Upur
keywords: Abnormal savda munziq decoction; diode array detector; high-performance liquid chromatography; phenolic compounds; uighur medicine