title: Characterization of a Gene Identified in Pathotype 5 of the Clubroot Pathogen Plasmodiophora brassicae.
abstract: Clubroot caused by Plasmodiophora brassicae is an important disease of crucifers worldwide. Isolates of the pathogen can be classified into pathotypes according to their pathogenicity on differential hosts. In this study, the presence or absence of all database-available nonhousekeeping P. brassicae genes (118 in total) were assessed by polymerase chain reaction (PCR) analysis in isolates belonging to five P. brassicae pathotypes (2, 3, 5, 6, and 8 according to Williams' differential set). One gene, designated Cr811, was present exclusively in the isolate of pathotype 5. This was further confirmed by dot blot hybridization and by PCR using alternative DNA preparations and primers. Reverse transcription quantitative PCR analysis indicated that in planta expression of Cr811 was up-regulated during canola infection, especially in the stage of secondary plasmodia. Primers specific to Cr811 could distinguish a field isolate of P. brassicae belonging to pathotype 5 from two other field isolates representing pathotypes 3 and 8. These findings suggest that Cr811 is a gene that is potentially involved in clubroot pathogenesis and that it also might serve as a molecular marker for differentiation of pathotype 5 from other pathotypes.
authors.affiliation: First author: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; first, second, and fifth authors: Crop Diversification Centre North, Alberta Agriculture and Rural Development, Edmonton, AB, T5Y 6H3, Canada; and first, third, and fourth authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: First author: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; first, second, and fifth authors: Crop Diversification Centre North, Alberta Agriculture and Rural Development, Edmonton, AB, T5Y 6H3, Canada; and first, third, and fourth authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Feng
authors.affiliation: First author: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; first, second, and fifth authors: Crop Diversification Centre North, Alberta Agriculture and Rural Development, Edmonton, AB, T5Y 6H3, Canada; and first, third, and fourth authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: V P
authors.initials: VP
authors.lastname: Manolii
authors.affiliation: First author: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; first, second, and fifth authors: Crop Diversification Centre North, Alberta Agriculture and Rural Development, Edmonton, AB, T5Y 6H3, Canada; and first, third, and fourth authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: S E
authors.initials: SE
authors.lastname: Strelkov
authors.affiliation: First author: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; first, second, and fifth authors: Crop Diversification Centre North, Alberta Agriculture and Rural Development, Edmonton, AB, T5Y 6H3, Canada; and first, third, and fourth authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
authors.forename: S-F
authors.initials: SF
authors.lastname: Hwang
mesh_terms: D029688:Brassica napus / Q000469:parasitology*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D060467:Disease Resistance; D022761:Gene Transfer, Horizontal; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000469:parasitology; D056909:Plasmodiophorida / Q000235:genetics* / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000235:genetics*; D015854:Up-Regulation; D014774:Virulence
chemical_list: D017931:DNA Primers; D015800:Protozoan Proteins
keywords: gene expression; horizontal gene transfer; resistance; virulence