title: Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens.
abstract: We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.
authors.affiliation: Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD.
authors.forename: Nuntra
authors.initials: N
authors.lastname: Suwantarat
authors.affiliation: Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD.
authors.forename: Justin B
authors.initials: JB
authors.lastname: Dalton
authors.affiliation: Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
authors.forename: Richard
authors.initials: R
authors.lastname: Lee
authors.affiliation: Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
authors.forename: Rachel
authors.initials: R
authors.lastname: Green
authors.affiliation: Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
authors.forename: Warda
authors.initials: W
authors.lastname: Memon
authors.affiliation: Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
authors.forename: Karen C
authors.initials: KC
authors.lastname: Carroll
authors.affiliation: Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; The Johns Hopkins Bayview Medical Center, Baltimore, MD.
authors.forename: Stefan
authors.initials: S
authors.lastname: Riedel
authors.affiliation: Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD. Electronic address: szhang28@jhmi.edu.
authors.forename: Sean X
authors.initials: SX
authors.lastname: Zhang
mesh_terms: D000946:Antigens, Fungal / Q000097:blood* / Q000134:cerebrospinal fluid*; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D003453:Cryptococcosis / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D011446:Prospective Studies; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D013997:Time Factors
chemical_list: D000946:Antigens, Fungal
keywords: Antigen; Cerebrospinal fluid; Cryptococcus; Lateral flow immunoassay; Serum