title: Characterization of Periodic Acid-Schiff-Positive Granular Deposits in the Hippocampus of SJL/J Mice.
abstract: Periodic acid-Schiff (PAS)-positive granular deposits in the hippocampus have been reported previously in certain inbred mouse strains such as C57BL/6 and the senescent-accelerated mouse prone-8. Here, we report for the first time that similar PAS-positive granules age dependently occur in SJL/J mice, a mouse strain, for instance, used for central nervous system disease research. Moreover, similar granules stained intensely positive with a polyclonal antibody directed against p75 neurotrophin receptor (p75(NTR)). Granular deposits were absent in young mice and developed with aging in CA1 and CA2 regions of the hippocampus. Interestingly, granules significantly diminished in SJL/J mice previously treated with cuprizone, a copper chelator, which is a useful model for toxic demyelination. The presented data support the idea that granules might be the result of an imbalance of redox-active metals and/or a dysregulation of complementary mechanisms that regulate their homeostasis in astrocyte-neuron coupling, respectively. It remains to be determined whether the unsuspected immunoreactivity for p75(NTR) represents a false-positive reaction or whether p75(NTR) is crucially involved in the pathogenesis of age-related hippocampal granular deposits in mice.
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany.
authors.forename: Armend
authors.initials: A
authors.lastname: Cana
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Herder
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Hansmann
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Beineke
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany wolfgang.baumgaertner@tiho-hannover.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany Center for Systems Neuroscience, Hannover, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Spitzbarth
mesh_terms: D000367:Age Factors; D000818:Animals; D003471:Cuprizone / Q000494:pharmacology; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010504:Periodic Acid / Q000737:chemistry*; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D012394:Rosaniline Dyes / Q000737:chemistry
chemical_list: D008996:Monoamine Oxidase Inhibitors; D017475:Receptors, Nerve Growth Factor; D012394:Rosaniline Dyes; C476677:Schiff's reagent; C517634:Ngfr protein, mouse; D010504:Periodic Acid; D003471:Cuprizone
keywords: SJL/J; aging; granules; hippocampus; p75 neurotrophin receptor; periodic acid-Schiff