title: Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species.
abstract: In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct banding patterns, allowing the correct identification of all 43 clinical and environmental isolates at the species level, what was confirmed by partial calmodulin gene sequence analyses. This technique is reproducible and provides the identification of all species of the Sporothrix complex with sufficient accuracy to be applied in clinical mycology laboratories as well as in epidemiological studies in order to obtain a better understanding of the epidemiology of sporotrichosis.
authors.affiliation: Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21045-900, Brazil manoel.marques@ini.fiocruz.br.
authors.forename: Manoel Marques Evangelista
authors.initials: MM
authors.lastname: Oliveira
authors.affiliation: Molecular and Environmental Biology Centre (CBMA), Universidade do Minho, 4710-057, Braga, Portugal.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Franco-Duarte
authors.affiliation: Department of Environmental and Biological Sciences - University of Messina, 98166, Italy.
authors.forename: Orazio
authors.initials: O
authors.lastname: Romeo
authors.affiliation: Molecular and Environmental Biology Centre (CBMA), Universidade do Minho, 4710-057, Braga, Portugal.
authors.forename: Célia
authors.initials: C
authors.lastname: Pais
authors.affiliation: Department of Environmental and Biological Sciences - University of Messina, 98166, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Criseo
authors.affiliation: Molecular and Environmental Biology Centre (CBMA), Universidade do Minho, 4710-057, Braga, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.affiliation: Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21045-900, Brazil.
authors.forename: Rosely Maria
authors.initials: RM
authors.lastname: Zancope-Oliveira
mesh_terms: D002147:Calmodulin; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D016533:Mycological Typing Techniques; D010802:Phylogeny; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013175:Sporothrix / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000453:epidemiology
chemical_list: D002147:Calmodulin; D017931:DNA Primers; D004271:DNA, Fungal
keywords: Sporothrix species complex; molecular identification; sporotrichosis