title: Sodium Fluoride Induces Apoptosis in H9c2 Cardiomyocytes by Altering Mitochondrial Membrane Potential and Intracellular ROS Level.
abstract: Chronic excessive fluoride intake is known to be toxic, and effects of long-term fluorosis on different organ systems have been examined. However, there are few studies about the effects of fluorosis on cardiovascular systems. Here, we studied the fluoride-induced apoptosis in H9c2 cells and determined the underlying molecular mechanisms including the cell viability, intracellular reactive oxygen species (ROS) level, the changes of mitochondrial membrane potential (ΔΨm), and the cell apoptosis. Sodium fluoride (NaF) at concentrations of 0, 2, 4, 8, and 16 mg/L was administered to cultured H9c2 cells for up to 48 h. After the treatment, H9c2 cells were collected and the associated parameters were measured by flow cytometry. Our study found that fluoride not only inhibited H9c2 cell proliferation but also induced cell apoptosis. With the increment of NaF concentration, the apoptotic rates and ROS generation were increased, while the ΔΨm was decreased. In summary, these data suggested that NaF-induced H9c2 cell apoptosis is mediated by direct increased intracellular ROS and downregulated ΔΨm.
authors.affiliation: Department of Biology, Taiyuan Normal University, (TYNU), Taiyuan, 030031, People's Republic of China, y_xiaoyan@yahoo.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yan
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Xianhui
authors.initials: X
authors.lastname: Hao
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Ren
authors.forename: Jiping
authors.initials: J
authors.lastname: Gao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Chang
authors.forename: Yulan
authors.initials: Y
authors.lastname: Qiu
authors.forename: Guohua
authors.initials: G
authors.lastname: Song
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D012969:Sodium Fluoride