title: Apoptotic effects of dillapiole on maturation of mouse oocytes, fertilization and fetal development.
abstract: Previously, we reported that dillapiole, a phenylpropanoid with antileishmanial, anti-inflammatory, antifungal and acaricidal activities, is a risk factor for normal embryonic development that triggers apoptotic processes in the inner cell mass of mouse blastocysts, leading to impaired embryonic development and cell viability. In the current study, we investigated the deleterious effects of dillapiole on mouse oocyte maturation, in vitro fertilization (IVF) and subsequent pre- and post-implantation development, both in vitro and in vivo. Notably, dillapiole induced significant impairment of mouse oocyte maturation, decrease in the IVF rate and inhibition of subsequent embryonic development in vitro. Pre-incubation of oocytes with dillapiole during in vitro maturation led to an increase in post-implantation embryo resorption and decrease in mouse fetal weight. In an in vivo animal model, 2.5, 5 or 10 μM dillapiole provided in drinking water caused a decrease in oocyte maturation and IVF, and led to deleterious effects on early embryonic development. Importantly, pre-incubation of oocytes with a caspase-3-specific inhibitor effectively blocked dillapiole-triggered deleterious effects, clearly implying that embryonic injury induced by dillapiole is mediated via a caspase-dependent apoptotic mechanism. To the best of our knowledge, this is the first study to establish the impact of dillapiole on maturation of mouse oocytes, fertilization and sequential embryonic development.
authors.affiliation: a Department of Life Science , National Pingtung University of Science and Technology , Pingtung , Taiwan .
authors.forename: Yan-Der
authors.initials: YD
authors.lastname: Hsuuw
authors.affiliation: b Department of Bioscience Technology and Center for Nanotechnology , Chung Yuan Christian University , Chung Li , Taiwan , and.|c Center for Biomedical Technology, Chung Yuan Christian University , Chung Li , Taiwan.
authors.forename: Wen-Hsiung
authors.initials: WH
authors.lastname: Chan
mesh_terms: D000498:Allyl Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D004149:Dioxoles / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D047109:Fetal Development / Q000187:drug effects; D020567:Fetal Weight / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy
chemical_list: D000498:Allyl Compounds; D004149:Dioxoles; C498255:5-allyl 6,7-dimethoxy 1,3-benzodioxole; D053148:Caspase 3
keywords: Apoptosis; dillapiole; embryonic development; oocyte maturation