title: Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle.
abstract: Chaperone-assisted selective autophagy (CASA) is a tension-induced degradation pathway essential for muscle maintenance. Impairment of CASA causes childhood muscle dystrophy and cardiomyopathy. However, the importance of CASA for muscle function in healthy individuals has remained elusive so far. Here we describe the impact of strength training on CASA in a group of healthy and moderately trained men. We show that strenuous resistance exercise causes an acute induction of CASA in affected muscles to degrade mechanically damaged cytoskeleton proteins. Moreover, repeated resistance exercise during 4 wk of training led to an increased expression of CASA components. In human skeletal muscle, CASA apparently acts as a central adaptation mechanism that responds to acute physical exercise and to repeated mechanical stimulation.
authors.affiliation: a Institute for Cell Biology; Rheinische Friedrich-Wilhelms-University Bonn ; Bonn , Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Ulbricht
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gehlert
authors.forename: Barbara
authors.initials: B
authors.lastname: Leciejewski
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schiffer
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
authors.forename: Jörg
authors.initials: J
authors.lastname: Höhfeld
mesh_terms: D000222:Adaptation, Physiological; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000502:physiology*; D001706:Biopsy; D003402:Creatine Kinase / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D064448:Filamins / Q000378:metabolism; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D018832:Molecular Chaperones / Q000502:physiology*; D009119:Muscle Contraction; D052097:Quadriceps Muscle / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; C578661:FLNC protein, human; D064448:Filamins; D008840:Microfilament Proteins; D018832:Molecular Chaperones; C503979:SYNPO2 protein, human; D003402:Creatine Kinase
keywords: BAG3; CASA, chaperone-assisted selective autophagy; CK, creatine kinase; HSPB8; TBS, tris-buffered saline; autophagy; chaperone; exercise; human subjects; mechanical tension; muscle; proteostasis; stress