title: Ultrasound-assisted (R)-phenylephrine whole-cell bioconversion by S. marcescens N10612.
abstract: The strain Serratia marcescens N10612 is used to perform the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone (HPMAE) to (R)-phenylephrine ((R)-PE), which is an ephedrine drug substitute. The use of an ultrasound approach is found to improve the efficiency of the (R)-PE bioconversion. The optimization of the (R)-PE bioconversion is carried out by means of statistical experiment design. The optimal conditions obtained are 1.0mM HPMAE, 18.68 g/L glucose and ultrasound power of 120 W, where the predicted specific rate of the (R)-PE bioconversion is 31.46 ± 2.22 (ìmol/h/g-cells) and the experimental specific rate is 33.27 ± 1.46 (ìmol/h/g-cells), which is 3-fold higher than for the operation under ultrasound power of 200 W (11.11 ìmol/h/g-cells) and 4.3-fold higher than for the shaking operation (7.69 ìmol/h/g-cells). The kinetics study of the bioconversion also shows that under the ultrasound operation, the optimal rate (Vmax) of the (R)-PE bioconversion increases from 7.69 to 11.11 (μmol/h/g-cells) and the substrate inhibition constant (KSi) increases from 1.063 mM for the shaking operation to 1.490 mM for ultrasound operation.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC.
authors.forename: Chi-Zong
authors.initials: CZ
authors.lastname: Zang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kan
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC.
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Yeh
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC.
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 40227, Taiwan, ROC. Electronic address: ycliu@dragon.nchu.edu.tw.
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
mesh_terms: D001711:Biotransformation; D007700:Kinetics; D010656:Phenylephrine / Q000737:chemistry* / Q000378:metabolism*; D012706:Serratia marcescens / Q000166:cytology* / Q000378:metabolism*; D013237:Stereoisomerism; D000069453:Ultrasonic Waves
chemical_list: D010656:Phenylephrine
keywords: (R)-phenylephrine; Bioconversion; Kinetics study; Optimization; Serratia marcescens; Ultrasound operation