title: Control of fire blight (Erwinia amylovora) on apple trees with trunk-injected plant resistance inducers and antibiotics and assessment of induction of pathogenesis-related protein genes.
abstract: Management of fire blight is complicated by limitations on use of antibiotics in agriculture, antibiotic resistance development, and limited efficacy of alternative control agents. Even though successful in control, preventive antibiotic sprays also affect non-target bacteria, aiding the selection for resistance which could ultimately be transferred to the pathogen Erwinia amylovora. Trunk injection is a target-precise pesticide delivery method that utilizes tree xylem to distribute injected compounds. Trunk injection could decrease antibiotic usage in the open environment and increase the effectiveness of compounds in fire blight control. In field experiments, after 1-2 apple tree injections of either streptomycin, potassium phosphites (PH), or acibenzolar-S-methyl (ASM), significant reduction of blossom and shoot blight symptoms was observed compared to water injected control trees. Overall disease suppression with streptomycin was lower than typically observed following spray applications to flowers. Trunk injection of oxytetracycline resulted in excellent control of shoot blight severity, suggesting that injection is a superior delivery method for this antibiotic. Injection of both ASM and PH resulted in the significant induction of PR-1, PR-2, and PR-8 protein genes in apple leaves indicating induction of systemic acquired resistance (SAR) under field conditions. The time separating SAR induction and fire blight symptom suppression indicated that various defensive compounds within the SAR response were synthesized and accumulated in the canopy. ASM and PH suppressed fire blight even after cessation of induced gene expression. With the development of injectable formulations and optimization of doses and injection schedules, the injection of protective compounds could serve as an effective option for fire blight control.
authors.affiliation: Tree Fruit Pathology Laboratory, Department of Plant, Soil and Microbial Sciences, Michigan State University East Lansing, MI, USA ; Applied Insecticide Toxicology Laboratory, Department of Entomology, Michigan State University East Lansing, MI, USA.
authors.forename: Srđan G
authors.initials: SG
authors.lastname: Aćimović
authors.affiliation: Tree Fruit Pathology Laboratory, Department of Plant, Soil and Microbial Sciences, Michigan State University East Lansing, MI, USA.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Tree Fruit Pathology Laboratory, Department of Plant, Soil and Microbial Sciences, Michigan State University East Lansing, MI, USA.
authors.forename: Gayle C
authors.initials: GC
authors.lastname: McGhee
authors.affiliation: Tree Fruit Pathology Laboratory, Department of Plant, Soil and Microbial Sciences, Michigan State University East Lansing, MI, USA.
authors.forename: George W
authors.initials: GW
authors.lastname: Sundin
authors.affiliation: Applied Insecticide Toxicology Laboratory, Department of Entomology, Michigan State University East Lansing, MI, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Wise
keywords: PR genes; SAR induction; acibenzolar-S-methyl; antibiotics; apple disease control; fire blight; potassium phosphites; trunk injection