title: Mechanism of intracellular detection of glucose through nonenzymatic and boronic acid functionalized carbon dots.
abstract: The objective of the research described here is to elucidate the fundamental mechanism by which the new class of "inert" non-enzymatic and boronic acid functionalized carbon dots-based sensors facilitate intracellular detection of glucose. The study suggests that the mechanism of detection of glucose involved selective assembly and fluorescence quenching of the carbon dots with excellent dynamic response to varying concentration of glucose within the biological range (1-100 mM). The strong dynamic response was related to high selectivity to biomolecules and inertness of carbon dots. Furthermore, the functionalization of carbon dots with boronic acid was the governing factor response for the passive character of the carbon dots. The study lays the foundation for the new field of carbon-based nanochemosensors.
authors.affiliation: Biomaterials and Macromolecular Science Laboratory, Center for Structural and Functional Materials Research and Innovation, and Department of Metallurgical and Materials Engineering, University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kiran
authors.affiliation: Biomaterials and Macromolecular Science Laboratory, Center for Structural and Functional Materials Research and Innovation, and Department of Metallurgical and Materials Engineering, University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USA.
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D005453:Fluorescence; D005947:Glucose / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D001897:Boronic Acids; D002244:Carbon; D005947:Glucose
keywords: boronic acid; carbon dots; fluorescence; glucose; nanochemosensors