title: Methods in molecular cardiology: suppression subtraction hybridisation.
abstract: Identification of differentially expressed genes in pathological tissues may provide more insight into the molecular mechanism involved in the development or progression of disease. Suppression subtraction hybridisation (SSH) allows the identification of novel, differentially expressed genes without prior knowledge about gene expression patterns or functional and biochemical protein characteristics. This review provides a general overview of the SSH method and gives two examples of SSH in the cardiovascular field.
authors.forename: M B
authors.initials: MB
authors.lastname: Smeets
authors.forename: G
authors.initials: G
authors.lastname: Pasterkamp
authors.forename: D P V
authors.initials: DP
authors.lastname: de Kleijn
keywords: SSH library; differentially expressed genes; identification; suppression subtraction hybridisation