title: [Human umbilical cord mesenchymal stem cells may differentiate into Leydig cells through conditioned medium induction].
abstract: OBJECTIVE
  To explore the feasibility of inducing human umbilical cord mesenchymal stem cells (HuMSCs) to differentiate into Leydig cells through conditioned medium derived from Leydig cells.
  
  
  METHODS
  HuMSCs and Leydig cells were obtained by tissue blocks culture attachment and enzymatic digestion respectively. HuMSCs were induced by conditioned medium of Leydig cells as an experiment group while those before induction were cultured as a control group. The expressions of LHR, 3β-HSD and StAR in the induced HuMSCs were determined by RT-PCR after 3, 7 and 10 days of culture; those of CYP11A1, CYP17A1 and 3β-HSD measured by immunofluorescence staining after 2 weeks; and that of 3β-HSD detected by Western blot after 4 weeks.
  
  
  RESULTS
  The experimental group showed positively expressed LHR, 3β-HSD and StAR at 3, 7 and 10 days, CYP11A1, CYP17A1 and 3β-HSD at 2 weeks, and 3β-HSD at 4 weeks, while the control group revealed negative expressions at all the time points.
  
  
  CONCLUSIONS
  Induced with conditioned culture medium derived from Leydig cells, HuMSCs are likely to differentiate into steroidogenic cells and eventually into Leydig cells.
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Xing
authors.forename: Ji-tao
authors.initials: JT
authors.lastname: Fan
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
mesh_terms: D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D006801:Humans; D007985:Leydig Cells / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned