title: CIDE-A gene expression in patients with obesity qualified for endovascular treatment of abdominal aorta aneurysm.
abstract: CIDE-A gene and the genes of LRP group play a key role in the regulation of the body weight and lipid metabolism in mammals. CIDE-A is defined as a potential human obesity gene and the LRP1 gene is associated with the development of abdominal aortic aneurysm (AAA). The aim of the study was to define the role of CIDE-A gene in patients with dyslipidemia and asymptomatic AAA.
  
  
  METHODS
  The study group consisted of 38 subjects, including 27 men and 11 women qualified for endovascular aneurysm repair (EVAR). The subjects with abdominal aortic aneurysm were enrolled in the study group, depending on the body mass index (BMI); in obese patients (BMI > 30). The control group (n = 16) included subjects without lipid disorders. One-step isolation of RNA from lymphocytes and adipose tissue cells was performed using the modified TRI method by Chomc-zynski and Sacchi, and then the gene expression was tested by real-time PCR.
  
  
  RESULTS
  The highest mean relative of the gene expression for CIDE-A was reported in subjects with the normal body weight. The lowest mean relative of the gene expression for CIDE-A was observed in the group of obese patients with aortic aneurysm and lipid disorders. A high negative correlation (r = -0.7101) in the gene expression for CIDE-A was observed in the group of obese patients with aortic aneurysm, depending on the BMI.
  
  
  CONCLUSIONS
  Due to the important role of the CIDE-A gene and Cide-A protein in the development of metabolic syndrome, obesity and the accompanying vascular lesions such as abdominal aortic an-eurysm, seen in this context, the tested gene and protein Cide-A represent a potential therapeutic target in these diseases.
authors.forename: Marcin
authors.initials: M
authors.lastname: Feldo
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocki
authors.forename: Jan
authors.initials: J
authors.lastname: Feldo
authors.forename: Sylwia
authors.initials: S
authors.lastname: Łukasik
authors.forename: Jacek
authors.initials: J
authors.lastname: Bogucki
authors.forename: Adam
authors.initials: A
authors.lastname: Skwarzyński
authors.forename: Jacek
authors.initials: J
authors.lastname: Wroński
authors.forename: Jan
authors.initials: J
authors.lastname: Kęsik
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zubilewicz
mesh_terms: D000368:Aged; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics* / Q000601:surgery*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D015992:Body Mass Index; D050171:Dyslipidemias / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000235:genetics*; D012307:Risk Factors; D016896:Treatment Outcome
chemical_list: D051017:Apoptosis Regulatory Proteins; C502539:CIDEA protein, human; C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1