title: De novo protein structure determination from near-atomic-resolution cryo-EM maps.
abstract: We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
authors.affiliation: 1] Graduate program in Biological Physics, Structure and Design, University of Washington, Seattle, Washington, USA. [2] Department of Biochemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Ray Yu-Ruei
authors.initials: RY
authors.lastname: Wang
authors.affiliation: 1] Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland. [2] Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kudryashev
authors.affiliation: The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Xueming
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Edward H
authors.initials: EH
authors.lastname: Egelman
authors.affiliation: Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Marek
authors.initials: M
authors.lastname: Basler
authors.affiliation: The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: 1] Department of Biochemistry, University of Washington, Seattle, Washington, USA. [2] Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA.
authors.forename: David
authors.initials: D
authors.lastname: Baker
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: DiMaio
mesh_terms: D002627:Chemistry, Physical / Q000379:methods*; D020285:Cryoelectron Microscopy; D008958:Models, Molecular; D009010:Monte Carlo Method; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013997:Time Factors
chemical_list: D011506:Proteins