title: Optimisation of gelatin extraction from Unicorn leatherjacket (Aluterus monoceros) skin waste: response surface approach.
abstract: Physical properties of gelatin extracted from Unicorn leatherjacket (Aluterus monoceros) skin, which is generated as a waste from fish processing industries, were optimised using Response Surface Methodology (RSM). A Box-Behnken design was used to study the combined effects of three independent variables, namely phosphoric acid (H3PO4) concentration (0.15-0.25 M), extraction temperature (40-50 °C) and extraction time (4-12 h) on different responses like yield, gel strength and melting point of gelatin. The optimum conditions derived by RSM for the yield (10.58%) were 0.2 M H3PO4 for 9.01 h of extraction time and hot water extraction of 45.83 °C. The maximum achieved gel strength and melting point was 138.54 g and 22.61 °C respectively. Extraction time was found to be most influencing variable and had a positive coefficient on yield and negative coefficient on gel strength and melting point. The results indicated that Unicorn leatherjacket skins can be a source of gelatin having mild gel strength and melting point.
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Mandakini Devi
authors.initials: MD
authors.lastname: Hanjabam
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Sathish Kumar
authors.initials: SK
authors.lastname: Kannaiyan
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Gaihiamngam
authors.initials: G
authors.lastname: Kamei
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Jitender Kumar
authors.initials: JK
authors.lastname: Jakhar
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Mithlesh Kumar
authors.initials: MK
authors.lastname: Chouksey
authors.affiliation: Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061 India.
authors.forename: Venkateshwarlu
authors.initials: V
authors.lastname: Gudipati
keywords: Gel strength; Gelatin; Melting point; Response surface methodology; Skin waste; Unicorn leatherjacket