title: Immunodiagnosis of canine visceral leishmaniasis using mimotope peptides selected from phage displayed combinatorial libraries.
abstract: ELISA and RIFI are currently used for serodiagnosis of canine visceral leishmaniasis (CVL). The accuracy of these tests is controversial in endemic areas where canine infections by Trypanosoma cruzi may occur. We evaluated the usefulness of synthetic peptides that were selected through phage display technique in the serodiagnosis of CVL. Peptides were chosen based on their ability to bind to IgGs purified from infected dogs pooled sera. We selected three phage clones that reacted only with those IgGs. Peptides were synthesized, polymerized with glutaraldehyde, and used as antigens in ELISA assays. Each individual peptide or a mix of them was reactive with infected dogs serum. The assay was highly sensitive and specific when compared to soluble Leishmania antigen that showed cross-reactivity with anti-T. cruzi IgGs. Our results demonstrate that phage display technique is useful for selection of peptides that may represent valuable synthetic antigens for an improved serodiagnosis of CVL.
authors.affiliation: Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Christina Monerat
authors.initials: CM
authors.lastname: Toledo-Machado
authors.affiliation: Departamento Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Ricardo Andrez Machado
authors.initials: RA
authors.lastname: de Avila
authors.affiliation: SysDiag CNRS-BioRad UMR 3145, Cap Delta/Parc Euromédecine, 1682 rue de la Valsière, CS 61003, 34184 Montpellier Cedex 4, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: NGuyen
authors.affiliation: SysDiag CNRS-BioRad UMR 3145, Cap Delta/Parc Euromédecine, 1682 rue de la Valsière, CS 61003, 34184 Montpellier Cedex 4, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Granier
authors.affiliation: Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Lilian Lacerda
authors.initials: LL
authors.lastname: Bueno
authors.affiliation: Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil.
authors.forename: Claudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.affiliation: Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Menezes-Souza
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Rubens Antonio
authors.initials: RA
authors.lastname: Carneiro
authors.affiliation: Departamento Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Chávez-Olórtegui
authors.affiliation: Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte 31270-901, MG, Brazil.
authors.forename: Ricardo Toshio
authors.initials: RT
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology*; D004285:Dogs; D018604:Epitope Mapping / Q000662:veterinary; D005260:Female; D007118:Immunoassay / Q000662:veterinary*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary*
chemical_list: D019151:Peptide Library; D010455:Peptides