title: Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products.
abstract: Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the contribution of wheat-based foods to the nutrition of the consumers. The aim of the present work was to develop a simple and rapid method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of seven water-soluble vitamins in various wheat-based food materials. The vitamins present in the test material were separated in less than 15 min by using a reverse-phase C18 column, and analyzed by positive ion electrospray selected reaction monitoring MS/MS. The MS response for all the vitamins was linear over the working range (0.05 to 9 μg/mL) with correlation coefficients ranging between 0.991 and 1. Limits of quantification in the different food materials ranged from 0.09 to 3.5 μg/g. Intra-day and inter-day precision were found satisfactory. The developed method was applied for the simultaneous analysis of the water-soluble vitamin natural content of different semi-coarse wheat flours and in their corresponding baking products.
authors.affiliation: INRA, UMR 1095, Génétique, Diversité et écophysiologie des céréales, 5 chemin de Beaulieu, 63039, Clermont-Ferrand, France, eric_nurit@hotmail.com.
authors.forename: Eric
authors.initials: E
authors.lastname: Nurit
authors.forename: Bernard
authors.initials: B
authors.lastname: Lyan
authors.forename: Agnès
authors.initials: A
authors.lastname: Piquet
authors.forename: Gérard
authors.initials: G
authors.lastname: Branlard
authors.forename: Estelle
authors.initials: E
authors.lastname: Pujos-Guillot
mesh_terms: D002247:Carbon Isotopes; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005433:Flour / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006868:Hydrolysis; D057230:Limit of Detection; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D014815:Vitamins