title: Macrophage immigration inhibitory factor promotes cell proliferation and inhibits apoptosis of cervical adenocarcinoma.
abstract: As a multifunctional cytokine, macrophage migration inhibitory factor (MIF) is associated with inflammation and tumorigenesis; however, the role of MIF in cervical adenocarcinoma (ADC) is not fully understood. In this study, we aimed to examine the expression of MIF in ADC and explore the mechanism of MIF in ADC progression. MIF expression was positively related to ADC clinicopathological features of carcinoma diameter and lymph node metastasis. MIF knockdown induced cell cycle arrest of G1/S transition in ADC cells, upregulation of the expressions of p21 and p27, and downregulation of the expressions of Cdk4, CyclinD2, and CyclinE2. In MIF knockdown cells, the expressions of proapoptotic proteins of Bax, caspase-3, cleaved caspase-3, and cleaved-PARP were upregulated, and the expressions of antiapoptotic proteins of Bcl-2, pAkt, and p53 were downregulated. It indicated that MIF knockdown inhibited cell proliferation and induced apoptosis in ADC cells. MIF might be a novel molecular marker in diagnosis and therapy of ADC.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, #58 Zhongshan er Road, Guangzhou, Guangdong, 510080, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Junxiu
authors.initials: J
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Xia
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Mian
authors.initials: M
authors.lastname: He
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D009363:Neoplasm Proteins; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human