title: Nerve growth factor protects retinal ganglion cells against injury induced by retinal ischemia-reperfusion in rats.
abstract: In this study, we investigated the protective effect of mouse nerve growth factor (NGF) on retinal ganglion cell (RGC) injury induced by retinal ischemia-reperfusion (RIR) in rats and explored its possible mechanisms of action. RIR caused a significant injury to RGCs and an obvious impairment of the inner retina functions, which could be seen from flash electroretinogram and flash visual evoked potential recordings. RIR also increased the expression of the apoptotic protein Bax while decreasing the expression of Bcl-2 and the phosphorylation of protein kinase B (Akt) in RGCs. Preinjection (i.m.) of NGF for 22 d reversed the injury induced by RIR and ameliorated the inner retina functions. NGF also reduced the expression of Bax and reversed the reduction of Bcl-2 and the phosphorylated Akt induced by RIR. These results indicate that NGF produces a neuroprotective effect on RGCs against RIR injury and the protective effect of NGF is mainly mediated by the PI-3K/Akt signaling pathway.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University , Guangzhou , China .
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.forename: Sufen
authors.initials: S
authors.lastname: Liao
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Rifang
authors.initials: R
authors.lastname: Liao
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Little
authors.forename: Jiangping
authors.initials: J
authors.lastname: Xu
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Feng
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Zheng
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D004596:Electroretinography; D005074:Evoked Potentials, Visual; D005260:Female; D008297:Male; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000517:prevention & control*; D012164:Retinal Diseases / Q000473:pathology / Q000517:prevention & control*; D012165:Retinal Ganglion Cells / Q000187:drug effects*; D014785:Vision, Ocular; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020932:Nerve Growth Factor; D019869:Phosphatidylinositol 3-Kinases
keywords: Nerve growth factor; PI-3K/Akt signaling pathway; retinal ganglion cells; retinal ischemia–reperfusion