title: TCP transcription factors are critical for the coordinated regulation of isochorismate synthase 1 expression in Arabidopsis thaliana.
abstract: Salicylic acid (SA) plays an important role in various aspects of plant development and responses to stresses. To elucidate the sophisticated regulatory mechanism of SA synthesis and signaling, we used a yeast one-hybrid system to screen for regulators of isochorismate synthase 1 (ICS1), a gene encoding the key enzyme in SA biosynthesis in Arabidopsis thaliana. A TCP family transcription factor AtTCP8 was initially identified as a candidate regulator of ICS1. The regulation of ICS1 by TCP proteins is supported by the presence of a typical TCP binding site in the ICS1 promoter. The binding of TCP8 to this site was confirmed by in vitro and in vivo assays. Expression patterns of TCP8 and its corresponding gene TCP9 largely overlapped with ICS1 under pathogen attack. A significant reduction in the expression of ICS1 during immune responses was observed in the tcp8 tcp9 double mutant. We also detected strong interactions between TCP8 and SAR deficient 1 (SARD1), WRKY family transcription factor 28 (WRKY28), NAC (NAM/ATAF1,ATAF2/CUC2) family transcription factor 019 (NAC019), as well as among TCP8, TCP9 and TCP20, suggesting a complex coordinated regulatory mechanism underlying ICS1 expression. Our results collectively demonstrate that TCP proteins are involved in the orchestrated regulation of ICS1 expression, with TCP8 and TCP9 being verified as major representatives.
authors.affiliation: State Key Laboratory of Genetic Engineering, Fudan Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Jiong
authors.initials: J
authors.lastname: Gao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xianxin
authors.initials: X
authors.lastname: Dong
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.forename: Guodong
authors.initials: G
authors.lastname: Ren
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Benke
authors.initials: B
authors.lastname: Kuai
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002827:Chorismic Acid / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism; D057865:Plant Immunity; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D020156:Salicylic Acid / Q000032:analysis / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C000604867:TCP8 protein, Arabidopsis; C000604868:TCP9 protein, Arabidopsis; D014157:Transcription Factors; C052985:isochorismic acid; D019751:Intramolecular Transferases; C058486:isochorismate synthase; D002827:Chorismic Acid; D020156:Salicylic Acid
keywords: Arabidopsis; ICS1 regulation; TCP transcription factor; plant immune response; protein-protein interaction