title: Quality evaluation of Perillae Folium by HPLC/PDA.
abstract: To establish a standard of quality control for Perillae Folium (Lamiaceae Family), four standard compounds including rosmarinic acid, elemicin, perillaldehyde, and dillapiole were evaluated by high-performance liquid chromatography (HPLC)/photodiode array (PDA). The four standards were analyzed with a Phenomenex Kinetex C18 (250 × 4.6 mm, 5 μm) column by gradient elution using 0.1 % formic acid in water and methanol as the mobile phase. The standards were quantified by HPLC/PDA from Perillae Folium, which included the leaf and twig of Perilla frutescens L. Britton var. acuta (Thunb.) Kudo or Perilla frutescens Britton var. crispa Decne. The method was successfully used in the analysis of Perillae Folium, and the linearity, recovery, precision, accuracy, stability, and robustness were satisfactory according to the validation results. In Perillae Folium samples, the average contents (wt%) of rosmarinic acid, elemicin, perillaldehyde, and dillapiole were 0.540, 0.059, 0.050, and 0.056 %, respectively. The results indicate that the established HPLC/PDA method is suitable for the quantitation and quality evaluation of Perillae Folium.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Republic of Korea.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
authors.forename: Je Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004353:Drug Evaluation, Preclinical / Q000379:methods; D019686:Lamiaceae; D031343:Perilla; D010936:Plant Extracts / Q000302:isolation & purification*; D018515:Plant Leaves
chemical_list: D010936:Plant Extracts