title: Sonoporation of adherent cells under regulated ultrasound cavitation conditions.
abstract: A sonoporation device dedicated to the adherent cell monolayer has been implemented with a regulation process allowing the real-time monitoring and control of inertial cavitation activity. Use of the cavitation-regulated device revealed first that adherent cell sonoporation efficiency is related to inertial cavitation activity, without inducing additional cell mortality. Reproducibility is enhanced for the highest sonoporation rates (up to 17%); sonoporation efficiency can reach 26% when advantage is taken of the standing wave acoustic configuration by applying a frequency sweep with ultrasound frequency tuned to the modal acoustic modes of the cavity. This device allows sonoporation of adherent and suspended cells, and the use of regulation allows some environmental parameters such as the temperature of the medium to be overcome, resulting in the possibility of cell sonoporation even at ambient temperature.
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France. Electronic address: pauline.muleki@gmail.com.
authors.forename: Pauline
authors.initials: P
authors.lastname: Muleki Seya
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France.
authors.forename: Manuela
authors.initials: M
authors.lastname: Fouqueray
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ngo
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Poizat
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Inserra
authors.affiliation: Inserm, U1032, LabTau, Lyon, F-69003, France; Université de Lyon, Lyon, F-69003, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Béra
mesh_terms: D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D006801:Humans; D015203:Reproducibility of Results; D014465:Ultrasonics / Q000379:methods*
keywords: Adherent cells; Cavitation regulation; Inertial cavitation; Sonoporation