title: [Oxidative stress and semen parameters in the serum and seminal plasma of infertile men with chronic viral hepatitis].
abstract: OBJECTIVE
  To investigate the malondialdehyde (MDA) level and paraoxonase-1 (PON-1) activity in the serum and seminal plasma of infertile men with chronic viral hepatitis and their influence on the semen parameters of the patients.
  
  
  METHODS
  We collected serum and semen samples from 42 infertile men, 45 infertile males with chronic viral hepatitis, and 50 healthy fertile men as controls. We measured the MDA level in the serum and seminal plasma by spectrophotometry, detected the PON-1 activity by spectrophotometry, and determined the sperm DNA fragmentation index (DFI) by acridine orange fluorescence staining.
  
  
  RESULTS
  The MDA level was significantly higher but the PON-1 activity remarkably lower in the serum and seminal plasma of the infertile males with chronic viral hepatitis than in the healthy controls and infertile patients (P <0.01 or P <0.05). Total sperm motility and sperm survival rate were significantly lower while the sperm DFI markedly higher in the former than in the latter two groups (P <0.01 or P <0.05). No statistically significant difference was found among the three groups in sperm concentration (P >0.05). The WBC counts in the semen of the infertile and infertile with chronic viral hepatitis groups were significantly higher than that in the health controls (P <0.05). The MDA level and PON-1 activity in the seminal plasma were positively correlated with those in the serum in the infertile males with chronic viral hepatitis (r=0.57 or 0.48, P <0.01).
  
  
  CONCLUSIONS
  Virus-induced chronic active hepatitis enhances oxidative stress in the reproductive system, aggravates sperm damage, and affects sperm quality parameters.
authors.forename: Dao-yuan
authors.initials: DY
authors.lastname: Gong
authors.forename: Zi-ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Hua-yi
authors.initials: HY
authors.lastname: Yao
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000032:analysis; D016022:Case-Control Studies; D053938:DNA Fragmentation; D005298:Fertility; D006525:Hepatitis, Viral, Human / Q000150:complications*; D006801:Humans; D007248:Infertility, Male / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D018384:Oxidative Stress; D012661:Semen; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa
chemical_list: D008315:Malondialdehyde; D043303:Aryldialkylphosphatase