title: Characterization of hospital and community-acquired respiratory syncytial virus in children with severe lower respiratory tract infections in Ho Chi Minh City, Vietnam, 2010.
abstract: BACKGROUND
  Human respiratory syncytial virus (RSV) is an important community and nosocomial pathogen in developed countries but data regarding the importance of RSV in developing countries are relatively scarce.
  
  
  METHODS
  During a 1-year surveillance study in 2010, we took serial samples from children admitted to the Emergency Unit of the Respiratory Ward of Children's Hospital 1 in Ho Chi Minh City, Vietnam. RSV was detected within 72 hours of admission to the ward in 26% (376/1439; RSV A: n = 320; RSV B: n = 54; and RSV A and B: n = 2). Among those negative in the first 72 hours after admission, 6.6% (25/377) acquired nosocomial RSV infection during hospitalization (RSV A: n = 22; and RSV B: n = 3).
  
  
  RESULTS
  Children with nosocomial RSV infection were younger (P = 0.001) and had a longer duration of hospitalization (P < 0.001). The rate of incomplete recovery among children with nosocomial RSV infection was significantly higher than among those without (P < 0.001). Phylogenetic analysis of partial G gene sequences obtained from 79% (316/401) of positive specimens revealed the co-circulation of multiple genotypes with RSV A NA1 being predominant (A NA1: n = 275; A GA5: n = 5; B BA3: n = 3; B BA9: n = 26; and B BA10: n = 7). The RSV A GA5 and RSV B BA3 genotypes have not been reported from Vietnam, previously.
  
  
  CONCLUSIONS
  Besides emphasizing the importance of RSV as a cause of respiratory infection leading to hospitalization in young children and as a nosocomial pathogen, data from this study extend our knowledge on the genetic diversity of RSV circulating in Vietnam.
authors.affiliation: Children's Hospital 1, Ho Chi Minh City, Vietnam.
authors.forename: Tran Anh
authors.initials: TA
authors.lastname: Tuan
authors.forename: Tran Tan
authors.initials: TT
authors.lastname: Thanh
authors.forename: Nguyen thi Thanh
authors.initials: Nt
authors.lastname: Hai
authors.forename: Le Binh Bao
authors.initials: le BB
authors.lastname: Tinh
authors.forename: Le thi Ngoc
authors.initials: le TN
authors.lastname: Kim
authors.forename: Lien Anh Ha
authors.initials: LA
authors.lastname: Do
authors.forename: Nguyen thi Thuy
authors.initials: Nt
authors.lastname: Chinh B'Krong
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Tham
authors.forename: Vu thi Ty
authors.initials: Vt
authors.lastname: Hang
authors.forename: Laura
authors.initials: L
authors.lastname: Merson
authors.forename: Jeremy
authors.initials: J
authors.lastname: Farrar
authors.forename: Tang Chi
authors.initials: TC
authors.lastname: Thuong
authors.forename: Menno D
authors.initials: MD
authors.lastname: de Jong
authors.forename: Constance
authors.initials: C
authors.lastname: Schultsz
authors.forename: H Rogier
authors.initials: HR
authors.lastname: van Doorn
mesh_terms: D017714:Community-Acquired Infections / Q000453:epidemiology / Q000821:virology*; D003428:Cross Infection / Q000821:virology*; D018592:Cross-Over Studies; D004636:Emergency Service, Hospital; D062665:Epidemiological Monitoring; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D008297:Male; D010802:Phylogeny; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012141:Respiratory Tract Infections / Q000150:complications / Q000821:virology*; D012621:Seasons; D017421:Sequence Analysis; D013997:Time Factors; D014744:Vietnam / Q000453:epidemiology
keywords: nosocomial infection; phylogenetics; respiratory syncytial virus