title: Practical Applications of in Vivo and ex Vivo MRI in Toxicologic Pathology Using a Novel High-performance Compact MRI System.
abstract: Magnetic resonance imaging (MRI) is widely used in preclinical research and drug development and is a powerful noninvasive method for assessment of phenotypes and therapeutic efficacy in murine models of disease. In vivo MRI provides an opportunity for longitudinal evaluation of tissue changes and phenotypic expression in experimental animal models. Ex vivo MRI of fixed samples permits a thorough examination of multiple digital slices while leaving the specimen intact for subsequent conventional hematoxylin and eosin (H&E) histology. With the advent of new compact MRI systems that are designed to operate in most conventional labs without the cost, complexity, and infrastructure needs of conventional MRI systems, the possibility of MRI becoming a practical modality is now viable. The purpose of this study was to investigate the capabilities of a new compact, high-performance MRI platform (M2™; Aspect Imaging, Israel) as it relates to preclinical toxicology studies. This overview will provide examples of major organ system pathologies with an emphasis on how compact MRI can serve as an important adjunct to conventional pathology by nondestructively providing 3-dimensional (3-D) digital data sets, detailed morphological insights, and quantitative information. Comparative data using compact MRI for both in vivo and ex vivo are provided as well as validation using conventional H&E.
authors.affiliation: Aspect Imaging, Shoham, Israel.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tempel-Brami
authors.affiliation: Aspect Imaging, Shoham, Israel yael@aspectimaging.com.
authors.forename: Yael S
authors.initials: YS
authors.lastname: Schiffenbauer
authors.affiliation: Tel Aviv University and Consultant in Toxicologic Pathology, Timrat, Tel Aviv, Israel.
authors.forename: Abraham
authors.initials: A
authors.lastname: Nyska
authors.affiliation: Harlan Biotech Israel, Nes Ziona, Israel.
authors.forename: Nati
authors.initials: N
authors.lastname: Ezov
authors.affiliation: Harlan Biotech Israel, Nes Ziona, Israel.
authors.forename: Itai
authors.initials: I
authors.lastname: Spector
authors.affiliation: Hadassah Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: Rinat
authors.initials: R
authors.lastname: Abramovitch
authors.affiliation: Maronpot Consulting, LLC, Raleigh, North Carolina, USA.
authors.forename: Robert R
authors.initials: RR
authors.lastname: Maronpot
mesh_terms: D000818:Animals; D006652:Histological Techniques / Q000379:methods*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010336:Pathology / Q000379:methods*; D014116:Toxicology / Q000379:methods*
keywords: MRI; ex vivo; in vivo; magnetic resonance histology (MRH); pathology; preclinical imaging.; toxicology