title: Addition of MCP-1 and MIP-3β to the IL-8 appraisal in peritoneal fluid enhances the probability of identifying women with endometriosis.
abstract: Chemokines have been associated with endometriosis. Our study was aimed at evaluating the levels of six chemokines--CXCL8 (IL-8), CXCL12 (SDF-1), CCL2 (MCP-1), CCL5 (RANTES), CCL19 (MIP-3β), and CCL21 (6-Ckine)--in the peritoneal fluid (PF) of patients with and controls without endometriosis by multiplexed cytokine assay. In this retrospective case-control study conducted at the Charité University Hospital, patients (n = 36) and controls (n = 27) were enrolled. The patients were separated into groups according to stage of the disease: I-II (n = 21), III-IV (n = 1 5), and according to clinical findings: peritoneal endometriosis (PE; n = 7), deep-infiltrating endometriosis (DIE) affecting the retrocervical area (n = 13) or the bowel/rectovaginal site (n = 14). The subjects were also separated according to the cycle phase: follicular (n = 14) or luteal (n = 8) and the previous use (n = 25) or not (n = 38) of hormones. PF was collected from all subjects (n = 63) consecutively during laparoscopy. The concentration of chemokines in the PF was assessed using Luminex(®) x-MAP(®) technology. Sensitivity and specificity were calculated. A model of multiple logistic regressions estimated the odds of endometriosis for each combination of the chemokines detected. We observed significantly higher concentrations of IL-8 (p < 0.001), MCP-1 (p = 0.014), and MIP-3β (p = 0.022) in the PF of women with endometriosis than in the controls. A joint evaluation revealed that elevated levels of the three chemokines had a positive endometriosis prediction value of 89.1%. The combined assessment of MCP-1, MIP-3β, and IL-8 concentration in PF improved the likelihood of identifying patients with endometriosis. Future studies should investigate this panel in peripheral blood samples.
authors.affiliation: Charité Universitätsmedizin Berlin - Gynecology Clinic, Campus Benjamin Franklin, Berlin, Germany; University of Sao Paulo, Faculty of Medicine - Department of Obstetrics and Gynecology, Brazil. Electronic address: giuborrelli@me.com.
authors.forename: G M
authors.initials: GM
authors.lastname: Borrelli
authors.affiliation: Charité Universitätsmedizin Berlin - Gynecology Clinic, Campus Benjamin Franklin, Berlin, Germany.
authors.forename: A M
authors.initials: AM
authors.lastname: Kaufmann
authors.affiliation: University of Sao Paulo, Faculty of Medicine - Department of Obstetrics and Gynecology, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Abrão
authors.affiliation: Charité Universitätsmedizin Berlin - Gynecology Clinic, Campus Benjamin Franklin, Berlin, Germany. Electronic address: sylvia.mechsner@charite.de.
authors.forename: S
authors.initials: S
authors.lastname: Mechsner
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D054415:Chemokine CCL19 / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism*; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008183:Luteal Phase; D012189:Retrospective Studies
chemical_list: C515460:CCL19 protein, human; C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D054415:Chemokine CCL19; D018932:Chemokine CCL2; D016209:Interleukin-8
keywords: Biomarkers; Chemokines; Endometriosis; Panel of markers