title: Systematic mining of salt-tolerant genes in halophyte-Zoysia matrella through cDNA expression library screening.
abstract: Though a large number of salt-tolerant genes were identified from Glycophyte in previous study, genes involved in salt-tolerance of halophyte were scarcely studied. In this report, an important halophyte turfgrass, Zoysia matrella, was used for systematic excavation of salt-tolerant genes using full-length cDNA expression library in yeast. Adopting the Gateway-compatible vector system, a high quality entry library was constructed, containing 3 × 10(6) clones with an average inserted fragments length of 1.64 kb representing a 100% full-length rate. The yeast expression library was screened in a salt-sensitive yeast mutant. The screening yielded dozens of salt-tolerant clones harboring 16 candidate salt-tolerant genes. Under salt-stress condition, these 16 genes exhibited different transcription levels. According to the results, we concluded that the salt-tolerance of Z. matrella might result from known genes involved in ion regulation, osmotic adjustment, as well as unknown pathway associated with protein folding and modification, RNA metabolism, and mitochondrial membrane translocase, etc. In addition, these results shall provide new insight for the future researches with respect to salt-tolerance.
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China; College of Ago-grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors.forename: Junqin
authors.initials: J
authors.lastname: Zong
authors.affiliation: College of Ago-grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Ago-grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Baoyun
authors.initials: B
authors.lastname: Hu
authors.affiliation: College of Ago-grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chuanming
authors.initials: C
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China. Electronic address: turfunit@aliyun.com.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D018076:DNA, Complementary; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000009:adverse effects; D015003:Yeasts
chemical_list: D018076:DNA, Complementary; D018749:RNA, Plant; D012965:Sodium Chloride
keywords: Salt-tolerance; Yeast; Zoysia matrella; cDNA library