title: A mine is a terrible thing to waste: high content, single cell technologies for comprehensive immune analysis.
abstract: In recent years, an incredible variety of single cell technologies have become available to analyze immune responses. These technologies include polychromatic flow cytometry, mass cytometry, highly multiplexed single cell qPCR, RNA sequencing, microtools, and high-resolution imaging. In this article, we review these platforms, describing their power and limitations for comprehensive analysis of the immune system. We relate the properties of these technologies to the various cellular states relevant to an immune response, in order to address which technologies are most appropriate for which settings.
authors.affiliation: Vaccine Research Center, National Institutes of Health, Bethesda, MD.
authors.forename: P K
authors.initials: PK
authors.lastname: Chattopadhyay
authors.forename: M
authors.initials: M
authors.lastname: Roederer
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007107:Immune System / Q000502:physiology*; D007962:Leukocytes / Q000276:immunology; D013058:Mass Spectrometry; D016133:Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis / Q000379:methods*; D059467:Transcriptome
keywords: basic (laboratory) research/science; cytokines/cytokine receptors; cytometry; cytotoxicity; disease; disease pathogenesis; editorial/personal viewpoint; flow; immunobiology; immunosuppression/immune modulation; infectious; monitoring: immune