title: Route of intrabacterial nanotransportation system for CagA in Helicobacter pylori.
abstract: Helicobacter pylori (H. pylori) possesses an intrabacterial nanotransportation system (ibNoTS) for transporting CagA and urease within the bacterial cytoplasm; this system is controlled by the extrabacterial environment. The transportation routes of the system have not yet been studied in detail. In this study, we demonstrated by immunoelectron microscopy that CagA localizes closely with the MreB filament in the bacterium, and MreB polymerization inhibitor A22 obstructs ibNoTS for CagA. These findings indicate that the route of ibNoTS for CagA is closely associated with the MreB filament. Because these phenomena were not observed in ibNoTS for urease, the route of ibNoTS for CagA is different from that of ibNoTS for urease as previously suggested. We propose that the route of ibNoTS for CagA is associated with the MreB filament in H. pylori.
authors.affiliation: Project Team for Study of Nanotransportation System, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan. wuhong@art.osaka-med.ac.jp.|Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan. wuhong@art.osaka-med.ac.jp.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Project Team for Study of Nanotransportation System, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.|Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Noritaka
authors.initials: N
authors.lastname: Iwai
authors.affiliation: Project Team for Study of Nanotransportation System, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.|Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.|Infection Control Office, Osaka Medical College Hospital, Osaka, Japan.
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Ooi
authors.affiliation: Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Ishihara
authors.affiliation: Project Team for Study of Nanotransportation System, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.|Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D021381:Protein Transport / Q000187:drug effects; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology; D014510:Urease / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori; C015799:2,6-dichlorobenzylthiopseudourea; D014510:Urease; D013890:Thiourea
keywords: A22; Acidic pH; CagA; Helicobacter pylori; Immunoelectron microscopy; Intrabacterial nanotransportation system; Localization; MreB; Shift; Urease