title: Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles).
abstract: We studied belowground and aboveground diversity and distribution of ectomycorrhizal (EM) fungal species colonizing Coccoloba uvifera (L.) L. (seagrape) mature trees and seedlings naturally regenerating in four littoral forests of the Guadeloupe island (Lesser Antilles). We collected 546 sporocarps, 49 sclerotia, and morphotyped 26,722 root tips from mature trees and seedlings. Seven EM fungal species only were recovered among sporocarps (Cantharellus cinnabarinus, Amanita arenicola, Russula cremeolilacina, Inocybe littoralis, Inocybe xerophytica, Melanogaster sp., and Scleroderma bermudense) and one EM fungal species from sclerotia (Cenococcum geophilum). After internal transcribed spacer (ITS) sequencing, the EM root tips fell into 15 EM fungal taxa including 14 basidiomycetes and 1 ascomycete identified. Sporocarp survey only weakly reflected belowground assessment of the EM fungal community, although 5 fruiting species were found on roots. Seagrape seedlings and mature trees had very similar communities of EM fungi, dominated by S. bermudense, R. cremeolilacina, and two Thelephoraceae: shared species represented 93 % of the taxonomic EM fungal diversity and 74 % of the sampled EM root tips. Furthermore, some significant differences were observed between the frequencies of EM fungal taxa on mature trees and seedlings. The EM fungal community composition also varied between the four investigated sites. We discuss the reasons for such a species-poor community and the possible role of common mycorrhizal networks linking seagrape seedlings and mature trees in regeneration of coastal forests.
authors.affiliation: Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal.|Département Systématique et Evolution, UMR 7205 CNRS OSEB Muséum national d'Histoire naturelle, CP 5045 rue Buffon, 75005, Paris, France.
authors.forename: Seynabou
authors.initials: S
authors.lastname: Séne
authors.affiliation: Laboratoire de Biologie et Physiologie Végétales, Faculté des Sciences Exactes et Naturelles, Université des Antilles et de la Guyane, BP 592, 97159, Pointe-à-Pitre, Guadeloupe, France.
authors.forename: Raymond
authors.initials: R
authors.lastname: Avril
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR113 INRA/AGRO-M/CIRAD/IRD/UM2-TA10/J, Campus International de Baillarguet, 34398, Montpellier cedex 5, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Chaintreuil
authors.affiliation: Département Systématique et Evolution, UMR 7205 CNRS OSEB Muséum national d'Histoire naturelle, CP 5045 rue Buffon, 75005, Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Geoffroy
authors.affiliation: Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal.
authors.forename: Cheikh
authors.initials: C
authors.lastname: Ndiaye
authors.affiliation: Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal.
authors.forename: Abdala Gamby
authors.initials: AG
authors.lastname: Diédhiou
authors.affiliation: IRD, UMR 195 LEMAR (UBO/CNRS/IRD/Ifremer), BP 1386, CP 18524, Dakar, Sénégal.
authors.forename: Oumar
authors.initials: O
authors.lastname: Sadio
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille Nord de France, BP 83, 59006, Lille cedex, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Courtecuisse
authors.affiliation: Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal.
authors.forename: Samba Ndao
authors.initials: SN
authors.lastname: Sylla
authors.affiliation: Département Systématique et Evolution, UMR 7205 CNRS OSEB Muséum national d'Histoire naturelle, CP 5045 rue Buffon, 75005, Paris, France.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Selosse
authors.affiliation: Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal. amadou.ba@univ-ag.fr.|Laboratoire de Biologie et Physiologie Végétales, Faculté des Sciences Exactes et Naturelles, Université des Antilles et de la Guyane, BP 592, 97159, Pointe-à-Pitre, Guadeloupe, France. amadou.ba@univ-ag.fr.|Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR113 INRA/AGRO-M/CIRAD/IRD/UM2-TA10/J, Campus International de Baillarguet, 34398, Montpellier cedex 5, France. amadou.ba@univ-ag.fr.
authors.forename: Amadou
authors.initials: A
authors.lastname: Bâ
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000502:physiology; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000502:physiology; D065928:Forests; D005800:Genes, Fungal; D019430:Guadeloupe; D064307:Microbiota; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000145:classification / Q000235:genetics / Q000502:physiology*; D019664:Polygonaceae / Q000382:microbiology*; D036226:Seedlings / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014197:Trees / Q000382:microbiology
keywords: Common mycorrhizal network; Fungal species richness; ITS sequencing; Regeneration; Seagrape coastal forests