title: Free radical-quenched SERS probes for detecting H2O2 and glucose.
abstract: For developing a free radical-quenched surface-enhanced Raman scattering (SERS) probe, starch, a linear molecule, was used as a protective layer to coat gold nanoshells (GNSs) as enhancement substrates and then, methylene blue (MB) was absorbed on the starch-coated GNSs as a free radical-responsive element. By detecting the change of the SERS intensity of MB on GNSs, the free radical-quenched SERS probes were used to detect H2O2, a less active reactive oxygen species (ROS), which was first converted to free radicals, a highly active ROS, to react with MB absorbed on GNSs to quench its SERS. The free radical-quenched SERS probe was also used to detect glucose in the presence of glucose oxidase which converted glucose to H2O2. The free radical-quenched SERS probe would be a versatile platform for detection of biochemical processes. The integration of optically changed molecules and optical enhancement of nanomaterials provided a way for advanced materials and analytical science.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China. dongjian@seu.edu.cn wqian@seu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Dong
authors.forename: Guoming
authors.initials: G
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhang
authors.forename: Weiping
authors.initials: W
authors.lastname: Qian
mesh_terms: D005609:Free Radicals / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D057145:Nanoshells / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D005609:Free Radicals; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D008751:Methylene Blue