title: High performance liquid chromatography coupled to mass spectrometry for profiling and quantitative analysis of folate monoglutamates in tomato.
abstract: Folates are essential micronutrients for animals as they play a major role in one carbon metabolism. Animals are unable to synthesize folates and obtain them from plant derived food. In the present study, a high performance liquid chromatography coupled to mass spectrometric (HPLC-MS/MS) method was developed for the high throughput screening and quantitative analysis of folate monoglutamates in tomato fruits. For folate extraction, several parameters were optimized including extraction conditions, pH range, amount of tri-enzyme and boiling time. After processing the extract was purified using ultra-filtration with 10 kDa membrane filter. The ultra-filtered extract was chromatographed on a RP Luna C18 column using gradient elution program. The method was validated by determining linearity, sensitivity and recovery. This method was successfully applied to folate estimation in spinach, capsicum, and garden pea and demonstrated that this method offers a versatile approach for accurate and fast determination of different folate monoglutamates in vegetables.
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: tyagi.kamal6672@gmail.com.
authors.forename: Kamal
authors.initials: K
authors.lastname: Tyagi
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: pravas43@gmail.com.
authors.forename: Pallawi
authors.initials: P
authors.lastname: Upadhyaya
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: supu.megha@gmail.com.
authors.forename: Supriya
authors.initials: S
authors.lastname: Sarma
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: vajirchem@gmail.com.
authors.forename: Vajir
authors.initials: V
authors.lastname: Tamboli
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: syellamaraju@gmail.com.
authors.forename: Yellamaraju
authors.initials: Y
authors.lastname: Sreelakshmi
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: rameshwar.sharma@gmail.com.
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005971:Glutamates / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018551:Solanum lycopersicum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005971:Glutamates; C083563:folate monoglutamate; D005492:Folic Acid
keywords: Folate extraction; Folates; HPLC–MS/MS; Tomato