title: Recent advances and concepts in substrate specificity determination of proteases using tailored libraries of fluorogenic substrates with unnatural amino acids.
abstract: Substrate specificity of proteases can be determined using several methods among which the most frequently used are positional scanning library, proteomics and phage display. Classic approaches can deliver information about preferences for natural amino acids in binding pockets of virtually all proteases. However, recent studies demonstrate the ability to obtain much more information by application of unnatural amino acids to positional scanning library approaches. This knowledge can be used for the design of more active and specific substrates, inhibitors and activity based probes. In this minireview we describe recent strategies and concepts for the design and application of fluorogenic substrates library tailored for exopeptidases and endopeptidases.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Rut
authors.forename: Paulina
authors.initials: P
authors.lastname: Kasperkiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Byzia
authors.forename: Marcin
authors.initials: M
authors.lastname: Poreba
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Groborz
authors.forename: Marcin
authors.initials: M
authors.lastname: Drag
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D019151:Peptide Library; D054852:Small Molecule Libraries; D010447:Peptide Hydrolases