title: The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan.
abstract: OBJECTIVE
  A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA, and the ability of isolated Pg to produce methyl mercaptan (CH3 SH) was determined to clarify the relationship between halitosis and Pg infection.
  
  
  METHODS
  CH3 SH concentrations were measured in patients using Oral Chroma. The production of CH3 SH by Pg standard and clinical strains was also measured in vitro. Real-time PCR was performed to compare the expression of mgl mRNA (which encoded l-methionine-a-deamino-g-mercaptomethane-lyase) among the Pg strains. The production of CH3 SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine.
  
  
  RESULTS
  The production of CH3 SH and the expression of mgl mRNA strongly correlated with each other in the presence of l-methionine. The expression of mgl mRNA by Pg W83 was strongly inhibited by magnoliaceae.
  
  
  CONCLUSIONS
  The production of CH3 SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ouhara
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kajiya
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: I J
authors.initials: IJ
authors.lastname: Savitri
authors.affiliation: Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tokunaga
authors.affiliation: Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shintani
authors.affiliation: Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ogawa
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hino
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shiba
authors.affiliation: Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.|Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000328:Adult; D000368:Aged; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000187:drug effects; D016866:Bacteroidaceae Infections / Q000378:metabolism / Q000382:microbiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D006209:Halitosis / Q000382:microbiology*; D006801:Humans; D029562:Magnoliaceae; D008297:Male; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; C005231:methylmercaptan; D008715:Methionine
keywords: Porphyromonas gingivalis; halitosis; periodontitis; volatile sulfur compounds