title: Human adipose derived stem cells induced cell apoptosis and s phase arrest in bladder tumor.
abstract: The aim of this study was to determine the effect of human adipose derived stem cells (ADSCs) on the viability and apoptosis of human bladder cancer cells. EJ and T24 cells were cocultured with ADSCs or cultured with conditioned medium of ADSCs (ADSC-CM), respectively. The cell counting and colony formation assay showed ADSCs inhibited the proliferation of EJ and T24 cells. Cell viability assessment revealed that the secretions of ADSCs, in the form of conditioned medium, were able to decrease cancer cell viability. Wound-healing assay suggested ADSC-CM suppressed migration of T24 and EJ cells. Moreover, the results of the flow cytometry indicated that ADSC-CM was capable of inducing apoptosis of T24 cells and inducing S phase cell cycle arrest. Western blot revealed ADSC-CM increased the expression of cleaved caspase-3 and cleaved PARP, indicating that ADSC-CM induced apoptosis in a caspase-dependent way. PTEN/PI3K/Akt pathway and Bcl-2 family proteins were involved in the mechanism of this reaction. Our study indicated that ADSCs may provide a promising and practicable manner for bladder tumor therapy.
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yu
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Boxing
authors.initials: B
authors.lastname: Su
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ge
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Zicheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing Shunyi District Hospital, Beijing 101300, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.affiliation: Qinzhou First People's Hospital, Qinzhou 535000, China.
authors.forename: Bingwei
authors.initials: B
authors.lastname: Huang
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Gong
authors.affiliation: National Urological Cancer Center, Beijing 100034, China ; Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin