title: Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells.
abstract: OBJECTIVE
  To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF).
  
  
  METHODS
  HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h.
  
  
  RESULTS
  MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h.
  
  
  CONCLUSIONS
  MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Cheng-Ye
authors.initials: CY
authors.lastname: Che
authors.affiliation: Shandong Lunan Eye Hospital, Eye Hospital of Shandong Medical College, Linyi 276002, Shandong Province, China.
authors.forename: Jing-Fen
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, School of Medicine, Pusan National University, Pusan 609-735, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Hu
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Gui-Qiu
authors.initials: GQ
authors.lastname: Zhao
keywords: Aspergillus fumigatus; human corneal epithelial cells; mannose-binding lectin