title: Rapid immunochemical analysis of the sulfonamide-sugar conjugated fraction of antibiotic contaminated honey samples.
abstract: A rapid high-throughput immunochemical screening (HtiS) procedure for the analysis of the sulfonamide (SA)-sugar conjugated fraction of antibiotic contaminated honey samples has been developed. Studies performed with this matrix have indicated that sulfonamide antibiotics are conjugated to sugars rapidly and quantitatively, providing samples with very low SA immunoreactivity. Therefore, sulfonamides must be first released before the analysis, and for this purpose, a simple and fast sample preparation procedure has been established consisting of hydrolyzing the sample for 5 min, adjusting the pH and buffering the sample prior to the immunochemical analysis. Under these conditions, honey samples could be directly analyzed without any additional sample treatment, other than dilution. Recovery values of the whole analytical procedure were greater than 85%. The analysis of the same samples without the hydrolysis provided recovery values below 5%. Selectivity studies performed in hydrolyzed honey samples revealed that nine relevant sulfonamide antibiotics can be detected with limit of detection (LOD) values below the action limits established by some EU countries (Belgium, 20 μg kg(-1), United Kingdom or Switzerland, 50 μg kg(-1)).
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Spain; CIBER de Bioingenieria, Biotemateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain(1).
authors.forename: A
authors.initials: A
authors.lastname: Muriano
authors.affiliation: UNISENSOR, Liege Wandre, Belgium(2).
authors.forename: V
authors.initials: V
authors.lastname: Chabottaux
authors.affiliation: Nestle Research Center, Lausanne, Switzerland(3).
authors.forename: J-M
authors.initials: JM
authors.lastname: Diserens
authors.affiliation: UNISENSOR, Liege Wandre, Belgium(2).
authors.forename: B
authors.initials: B
authors.lastname: Granier
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Spain; CIBER de Bioingenieria, Biotemateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain(1).
authors.forename: F
authors.initials: F
authors.lastname: Sanchez-Baeza
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Spain; CIBER de Bioingenieria, Biotemateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain(1). Electronic address: pilar.marco@cid.csic.es.
authors.forename: M-P
authors.initials: MP
authors.lastname: Marco
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D006868:Hydrolysis; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002241:Carbohydrates; D013449:Sulfonamides
keywords: Class-selective antibodies; Honey; Hydrolysis; Immunoassay; Magnetic particles; Sulfonamide antibiotics; Sulfonamide-sugar conjugates