title: The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase.
abstract: Despite the growing importance of the mass spectrometry of membrane proteins, it is not known how their transfer from solution into vacuum affects their stability and structure. To address this we have carried out a systematic investigation of ten membrane proteins solubilized in different detergents and used mass spectrometry to gain physicochemical insight into the mechanism of their ionization and desolvation. We show that the chemical properties of the detergents mediate the charge state, both during ionization and detergent removal. Using ion mobility mass spectrometry, we monitor the conformations of membrane proteins and show how the surface charge density dictates the stability of folded states. We conclude that the gas-phase stability of membrane proteins is increased when a greater proportion of their surface is lipophilic and is consequently protected by the physical presence of the micelle.
authors.affiliation: Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QZ (UK).
authors.forename: Eamonn
authors.initials: E
authors.lastname: Reading
authors.forename: Idlir
authors.initials: I
authors.lastname: Liko
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Allison
authors.forename: Justin L P
authors.initials: JL
authors.lastname: Benesch
authors.forename: Arthur
authors.initials: A
authors.lastname: Laganowsky
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
mesh_terms: D020346:Aquaporins / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D011487:Protein Conformation; D055550:Protein Stability
chemical_list: D020346:Aquaporins; D003902:Detergents; D029968:Escherichia coli Proteins; D005740:Gases; D008565:Membrane Proteins; D008823:Micelles; C106425:aqpZ protein, E coli
keywords: ion mobility spectrometry; mass spectrometry; membrane proteins; micelles; structural biology