title: The ACE I/D polymorphism is associated with nitric oxide metabolite and blood pressure levels in healthy Mexican men.
abstract: The I/D insertion/deletion polymorphism of the angiotensin-converting enzyme has been related to hypertension. This polymorphism also seems to have gender related implications. Angiotensin II contributes to the production and release of oxygen reactive species that react with nitric oxide, inactivating its effects.
  
  
  OBJECTIVE
  To establish whether the ACE I/D polymorphism correlates with nitric oxide plasma metabolites in healthy men and women.
  
  
  METHODS
  Among 896 subjects between 18 and 30 years of age range, 138 fulfilled inclusion criteria. The polymorphism was identified by polymerase chain reaction, and blood nitric oxide metabolites were analyzed following the method described by Bryan.
  
  
  RESULTS
  Both systolic and diastolic arterial pressures were higher in men than in women (107/67 vs. 101/65 mm Hg, p<0.001). In terms of the ACE gene, there were differences in the concentration of nitric oxide metabolites in men with the I/D and D/D genotypes when compared to carriers of the I/I genotype (33.55 and 29.23 vs. 53.74 pmol/ml; p=<0.05), while there were no significant differences in women when compared by genotype. Men with the D/D genotype had higher systolic blood pressure than I/D carriers (111 vs. 104 mm Hg, p<0.05). We observed no arterial blood pressure differences in women when grouped by ACE genotype.
  
  
  CONCLUSIONS
  The ACE D/D genotype was associated with nitric oxide metabolite levels and systolic blood pressure in clinically healthy men while it had no effect in women.
authors.affiliation: Department of Echocardiography, National Cardiology Institute "Ignacio Chávez", Mexico. Electronic address: vazzny74@yahoo.com.
authors.forename: Nydia
authors.initials: N
authors.lastname: Avila-Vanzzini
authors.affiliation: Department of Endocrinology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Posadas-Romero
authors.affiliation: Department of Endocrinology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Maria Del Carmen
authors.initials: Mdel C
authors.lastname: Gonzalez-Salazar
authors.affiliation: Department of Endocrinology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Carolina
authors.initials: C
authors.lastname: Maass-Iturbide
authors.affiliation: Department of Magnetic Resonance, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Melendez-Ramirez
authors.affiliation: Department of Molecular Biology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Perez-Mendez
authors.affiliation: Department of Pharmacology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Del Valle-Mondragon
authors.affiliation: Department of Physiology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Felipe
authors.initials: F
authors.lastname: Masso-Rojas
authors.affiliation: Department of Physiology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: Elvira
authors.initials: E
authors.lastname: Varela Lopez
authors.affiliation: Médica Sur Clinical Foundation, Mexico City, Mexico.
authors.forename: Hector
authors.initials: H
authors.lastname: Herrera-Bello
authors.affiliation: Hemodynamic Department, National Cardiology Institute, "Ignacio Chávez", Mexico.
authors.forename: Ramon
authors.initials: R
authors.lastname: Villavicencio Fernandez
authors.affiliation: Department of Molecular Biology, National Cardiology Institute "Ignacio Chávez", Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Cruz-Robles
mesh_terms: D000293:Adolescent; D000328:Adult; D001794:Blood Pressure; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D009569:Nitric Oxide / Q000097:blood* / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D009569:Nitric Oxide; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
keywords: ACE I/D polymorphism; Healthy subjects; Nitric oxide; Oxido Nítrico; Población sana; Polimorfismo I/D ECA