title: Citrobacter rodentium-induced colitis: A robust model to study mucosal immune responses in the gut.
abstract: Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells.
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA.
authors.forename: Ekaterina P
authors.initials: EP
authors.lastname: Koroleva
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA.
authors.forename: Sydney
authors.initials: S
authors.lastname: Halperin
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA.
authors.forename: Ekaterina O
authors.initials: EO
authors.lastname: Gubernatorova
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA.
authors.forename: Elise
authors.initials: E
authors.lastname: Macho-Fernandez
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA.
authors.forename: Cody M
authors.initials: CM
authors.lastname: Spencer
authors.affiliation: Trudeau Institute, Saranac Lake, NY 12983, USA; Engelhardt Institute of Molecular Biology, Moscow, Russia. Electronic address: atumanov@trudeauinstitute.org.
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Tumanov
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D044082:Citrobacter rodentium / Q000276:immunology*; D003092:Colitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D004756:Enterobacteriaceae Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D006965:Hyperplasia / Q000276:immunology / Q000382:microbiology; D018928:Immunity, Mucosal / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
keywords: Citrobacter rodentium; Infection-induced colitis; Mucosal model of inflammation