title: The preparation of chicken kidney cell cultures for virus propagation.
abstract: Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is described. This technique routinely gives high cell yield together with high viability and the resultant adherent primary cultures can be used for virus growth and plaque formation.
authors.affiliation: The Pirbright Institute, High Street, Compton, Newbury, Berkshire, RG20 7NN, UK, ruth.hennion@pirbright.ac.uk.
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Hennion
authors.forename: Gillian
authors.initials: G
authors.lastname: Hill
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002645:Chickens; D017934:Coronavirus / Q000502:physiology*; D007668:Kidney / Q000166:cytology*; D014776:Virus Cultivation / Q000379:methods