title: Analysis of miRNA expression patterns in human and mouse hepatocellular carcinoma cells.
abstract: OBJECTIVE
  Hepatocellular carcinoma (HCC), one of the most common malignancies in adults displays aberrant miRNA expression during its pathogenesis. We assessed expression of miRNA in surgically resected human HCC of an early stage and murine HCC with a high malignancy in order to find miRNA overexpressed in HCC regardless of tumor stage and underlying etiology. Further, the role of the deregulated miRNA in HCC pathogenesis was investigated.
  
  
  METHODS
  miRNA were isolated from HCC tissues and surrounding non-tumorous tissues from HCC patients and a murine transgenic model of HCC. A quantitative reverse transcription polymerase chain reaction was performed to determine expression levels of miRNA. Human HCC cell lines stably expressing individual miRNA were generated to investigate the biological function of overexpressed miRNA.
  
  
  RESULTS
  We found that levels of miR-221, -181b-1, -155-5p, -25 and -17-5p were significantly upregulated in both human and murine HCC regardless of tumor stage, underlying etiology or the presence of fibrosis. Using HCC cell lines stably expressing respective miRNA, we found that miR-221 increased the proliferation of hepatoma cells, while miR-17-5p induced cell migration.
  
  
  CONCLUSIONS
  We identified miRNA that are consistently upregulated in HCC. The overexpressed miRNA could potentially be used as a bona fide biomarker for HCC.
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sinhwa
authors.initials: S
authors.lastname: Baek
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kyung Joo
authors.initials: KJ
authors.lastname: Cho
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Brain Korea 21 Project for Medical Science College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Hye-Lim
authors.initials: HL
authors.lastname: Ju
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Brain Korea 21 Project for Medical Science College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Moon
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Brain Korea 21 Project for Medical Science College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Brain Korea 21 Project for Medical Science College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Sook In
authors.initials: SI
authors.lastname: Chung
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jun Yong
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Ki Hong
authors.initials: KH
authors.lastname: Choi
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kwang-Hyub
authors.initials: KH
authors.lastname: Han
authors.affiliation: Liver Cirrhosis Clinical Research Center, Yonsei University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Simon Weonsang
authors.initials: SW
authors.lastname: Ro
keywords: hepatocellular carcinoma; hepatoma cell line; miRNA; quantitative reverse transcription polymerase chain reaction; wound-healing assay