title: A therapeutic strategy for spinal cord defect: human dental follicle cells combined with aligned PCL/PLGA electrospun material.
abstract: Stem cell implantation has been utilized for the repair of spinal cord injury; however, it shows unsatisfactory performance in repairing large scale lesion of an organ. We hypothesized that dental follicle cells (DFCs), which possess multipotential capability, could reconstruct spinal cord defect (SCD) in combination with biomaterials. In the present study, mesenchymal and neurogenic lineage characteristics of human DFCs (hDFCs) were identified. Aligned electrospun PCL/PLGA material (AEM) was fabricated and it would not lead to cytotoxic reaction; furthermore, hDFCs could stretch along the oriented fibers and proliferate efficiently on AEM. Subsequently, hDFCs seeded AEM was transplanted to restore the defect in rat spinal cord. Functional observation was performed but results showed no statistical significance. The following histologic analyses proved that AEM allowed nerve fibers to pass through, and implanted hDFCs could express oligodendrogenic lineage maker Olig2 in vivo which was able to contribute to remyelination. Therefore, we concluded that hDFCs can be a candidate resource in neural regeneration. Aligned electrospun fibers can support spinal cord structure and induce cell/tissue polarity. This strategy can be considered as alternative proposals for the SCD regeneration studies.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Xinghan
authors.identifier: 0000-0002-7671-6326
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Zongting
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Pedodontics, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China ; Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002478:Cells, Cultured; D003795:Dental Sac / Q000502:physiology*; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000627:therapeutic use*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D011100:Polyglycolic Acid / Q000627:therapeutic use*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology* / Q000628:therapy*; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid