title: Bifunctional glass membrane designed to interface SDS-PAGE separations of proteins with the detection of peptides by mass spectrometry.
abstract: We describe the construction and characterization of a novel membrane designed to allow proteins separated by gel electrophoresis (SDS-PAGE) to be detected as peptides by mass spectrometry in an efficient and comprehensive manner. The key attribute of the membrane is a bifunctional design that allows for the digestion of protein(s) and retention of the resulting peptides with minimal lateral diffusion. Silane chemistries are used to differentially treat the opposing surfaces of a glass filter paper to enable this unique capability.
authors.affiliation: †SimulTOF Systems, 60 Union Avenue, Sudbury, Massachusetts 01776, United States.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hattan
authors.affiliation: ‡Toxikon Corporation, 15 Wiggins Avenue, Bedford, Massachusetts 01730, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: †SimulTOF Systems, 60 Union Avenue, Sudbury, Massachusetts 01776, United States.
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Parker
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation; D005898:Glass / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D008567:Membranes, Artificial; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012821:Silanes / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D010455:Peptides; D011506:Proteins; D012821:Silanes