title: Measurements of rhodopsin diffusion within signaling membrane microcompartments in live photoreceptors.
abstract: High-resolution multiphoton imaging of live cells has become an invaluable method to study protein dynamics in highly compartmentalized subcellular environments. Here we describe procedures that we recently developed to quantify rhodopsin mobility within and between retinal rod photoreceptor light signaling microcompartments, the disc membrane lobules, using multiphoton fluorescence relaxation after photoconversion.
authors.affiliation: Department of Ophthalmology and the Center for Vision Research, State University of New York Upstate Medical University, Syracuse, NY, USA.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Najafi
authors.forename: Peter D
authors.initials: PD
authors.lastname: Calvert
mesh_terms: D010786:Photoreceptor Cells / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D043562:Receptors, G-Protein-Coupled; D012243:Rhodopsin