title: Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier.
abstract: Using and engineering amyloid as nanomaterials are blossoming trends in bionanotechnology. Here, we show our discovery of an amyloid structure, termed "amyloid-like nanosheet," formed by a key amyloid-forming segment of Alzheimer's Aβ. Combining multiple biophysical and computational approaches, we proposed a structural model for the nanosheet that is formed by stacking the amyloid fibril spines perpendicular to the fibril axis. We further used the nanosheet for laboratorial retroviral transduction enhancement and directly visualized the presence of virus on the nanosheet surface by electron microscopy. Furthermore, based on our structural model, we designed nanosheet-forming peptides with different functionalities, elucidating the potential of rational design for amyloid-based materials with novel architecture and function.
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Surface Physics, Key Laboratory for Computational Physical Sciences (Ministry of Education), Department of Physics, Fudan University, Shanghai 200433, China;
authors.forename: Wenhui
authors.initials: W
authors.lastname: Xi
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Fang
authors.initials: F
authors.lastname: Luo
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Man
authors.initials: M
authors.lastname: Zou
authors.affiliation: National Center for Protein Science (Shanghai), Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201210, China; and.
authors.forename: Mi
authors.initials: M
authors.lastname: Cao
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Surface Physics, Key Laboratory for Computational Physical Sciences (Ministry of Education), Department of Physics, Fudan University, Shanghai 200433, China; liulab@sioc.ac.cn ghwei@fudan.edu.cn zhangyi@sinap.ac.cn.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Wei
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China liulab@sioc.ac.cn ghwei@fudan.edu.cn zhangyi@sinap.ac.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; liulab@sioc.ac.cn ghwei@fudan.edu.cn zhangyi@sinap.ac.cn.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D049329:Nanostructures; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D012190:Retroviridae / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D014161:Transduction, Genetic
chemical_list: D000682:Amyloid; D010455:Peptides
keywords: beta-amyloid; functional amyloid material; nanosheet; peptide self-assembly; retrovirus transduction