title: [Modulation of apoptosis in mononuclear cells with different variants of polymorphism C-262T in catalase gene].
abstract: Catalase is a clue enzyme metabolizing hydrogen peroxide under conditions of oxidative stress. It is known that the C-262T polymorphism in catalase gene is implicated in higher risk of some diseases onset. In this article we have evaluated the viability of mononuclear leukocytes isolated from patients with different variants of C-262T polymorphism in catalase gene (CC, CT, TT) under conditions of hydrogen peroxide induced oxidative stress. It has been revealed that cells with TT variant of polymorphism have lower viability in the presence of hydrogen peroxide in a concentration of 1000 μM/L in comparison with cells having CC and CT genotypes. Furthermore, unlike CC and CT variants, cells with TT polymorphism showed no activation of mitochondrial apoptotic pathway even with hydrogen peroxide at a concentration of 1 mM. The data obtained may explain the increased risk of diseases related with the activity of the antioxidant system in patients with 262 TT promoter polymorphism in catalase gene.
authors.forename: A V
authors.initials: AV
authors.lastname: Komina
authors.forename: T G
authors.initials: TG
authors.lastname: Ruksha
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005838:Genotype; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D061251:Primary Cell Culture; D011401:Promoter Regions, Genetic; D018079:Receptors, GABA / Q000235:genetics* / Q000378:metabolism
chemical_list: D018079:Receptors, GABA; C499229:TSPO protein, human; D006861:Hydrogen Peroxide; D002374:Catalase