title: Expression of aquaporin-1, aquaporin-3, and aquaporin-5 correlates with nodal metastasis in colon cancer.
abstract: OBJECTIVE
  The clinical significance of aquaporin-1 (AQP1), aquaporin-3 (AQP3), and aquaporin-5 (AQP5) expression was analyzed in a large number of patients with colon cancer.
  
  
  METHODS
  AQP1, AQP3, and AQP5 expression was investigated based on the immunohistochemistry of tissue microarray specimens from 486 colon cancer patients who underwent curative surgery. Scores were given to the staining intensity and percentage of positive cells, and the staining score was defined as the sum of these scores then used to categorize the AQP expression as negative, weakly AQP-positive, or strongly AQP-positive.
  
  
  RESULTS
  A total of 298 (61.3%) patients were identified as strongly AQP1-positive (staining score ≥ 6), while 38 (7.8%) were strongly AQP3-positive and 145 (29.8%) were strongly AQP5-positive. The overexpression of AQP1, AQP3, and AQP5 was significantly correlated with lymph node metastasis in a multivariate logistic analysis (AQP1, p = 0.026; AQP3, p = 0.023; AQP5, p = 0.003). While the multivariate survival analysis, which included age, histology, TNM stage, and CEA level showed that the expression of AQP1, AQP3, and AQP5 had no effect on the overall survival and disease-free survival.
  
  
  CONCLUSIONS
  The current study found a significant correlation between AQP1, AQP3, and AQP5 expression and lymph node metastasis in patients with surgically resected colon cancer.
authors.affiliation: Department of Oncology/Hematology, Kyungpook National University Medical Center, Kyungpook National University School of Medicine, Daegu, South Korea.
authors.forename: Byung Woog
authors.initials: BW
authors.lastname: Kang
authors.forename: Jong Gwang
authors.initials: JG
authors.lastname: Kim
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yee Soo
authors.initials: YS
authors.lastname: Chae
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Jeong
authors.forename: Ghil Suk
authors.initials: GS
authors.lastname: Yoon
authors.forename: Soo Yeun
authors.initials: SY
authors.lastname: Park
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jun Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Gyu-Seog
authors.initials: GS
authors.lastname: Choi
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Jeong
mesh_terms: D000328:Adult; D000368:Aged; D051398:Aquaporin 1 / Q000378:metabolism*; D051400:Aquaporin 3 / Q000378:metabolism*; D051402:Aquaporin 5 / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D046888:Tissue Array Analysis
chemical_list: C496020:AQP1 protein, human; C496027:AQP3 protein, human; C496036:AQP5 protein, human; D051402:Aquaporin 5; D051398:Aquaporin 1; D051400:Aquaporin 3