title: The biophysical properties of Basal lamina gels depend on the biochemical composition of the gel.
abstract: The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of mice. Here, we compare four different commercial variants of this ECM, which have all been purified according to the same protocol. Nevertheless, in those gels, we detect strong differences in the migration behavior of leukocyte cells as well as in the Brownian motion of nanoparticles. We show that these differences correlate with the mechanical properties and the microarchitecture of the gels which in turn arise from small variations in their biochemical composition.
authors.affiliation: Institute of Medical Engineering IMETUM, Technische Universität München, Boltzmannstrasse 11, 85748, Garching, Germany; Department of Mechanical Engineering, Technische Universität München, Boltzmannstrasse 15, 85748, Garching, Germany.
authors.forename: Fabienna
authors.initials: F
authors.lastname: Arends
authors.affiliation: Institute of Medical Engineering IMETUM, Technische Universität München, Boltzmannstrasse 11, 85748, Garching, Germany; Department of Mechanical Engineering, Technische Universität München, Boltzmannstrasse 15, 85748, Garching, Germany.
authors.forename: Constantin
authors.initials: C
authors.lastname: Nowald
authors.affiliation: Department of Pharmacy-Center for Drug Research, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377, München, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Pflieger
authors.affiliation: Institute of Medical Engineering IMETUM, Technische Universität München, Boltzmannstrasse 11, 85748, Garching, Germany; Department of Mechanical Engineering, Technische Universität München, Boltzmannstrasse 15, 85748, Garching, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Boettcher
authors.affiliation: Department of Pharmacy-Center for Drug Research, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377, München, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zahler
authors.affiliation: Institute of Medical Engineering IMETUM, Technische Universität München, Boltzmannstrasse 11, 85748, Garching, Germany; Department of Mechanical Engineering, Technische Universität München, Boltzmannstrasse 15, 85748, Garching, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Lieleg
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry* / Q000378:metabolism; D055592:Biophysical Phenomena; D002465:Cell Movement; D004058:Diffusion; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D018922:HL-60 Cells; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D053758:Nanoparticles / Q000378:metabolism
chemical_list: D005782:Gels