title: [Bioremediation of oil-polluted soils: using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation].
abstract: We compared data on the extent of bioremediation in soils polluted with oil. The data were obtained using conventional methods of hydrocarbon determination: extraction gas chromatography-mass spectrometry, extraction IR spectroscopy, and extraction gravimetry. Due to differences in the relative abundances of the stable carbon isotopes (13C/12C) in oil and in soil organic matter, these ratios could be used as natural isotopic labels of either substance. Extraction gravimetry in combination with characteristics of the carbon isotope composition of organic products in the soil before and after bioremediation was shown to be the most informative approach to an evaluation of soil bioremediation. At present, it is the only method enabling quantification of the total petroleum hydrocarbons in oil-polluted soil, as well as of the amounts of hydrocarbons remaining after bioremediation and those microbially transformed into organic products and biomass.
authors.forename: A M
authors.initials: AM
authors.lastname: Ziakun
authors.forename: E S
authors.initials: ES
authors.lastname: Brodskiĭ
authors.forename: B P
authors.initials: BP
authors.lastname: Baskunov
authors.forename: V N
authors.initials: VN
authors.lastname: Zakharchenko
authors.forename: V P
authors.initials: VP
authors.lastname: Peshenko
authors.forename: A E
authors.initials: AE
authors.lastname: Filonov
authors.forename: A A
authors.initials: AA
authors.lastname: Vetrova
authors.forename: A A
authors.initials: AA
authors.lastname: Ivanova
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D001673:Biodegradation, Environmental; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D010578:Petroleum / Q000032:analysis* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D002247:Carbon Isotopes; D006838:Hydrocarbons; D010578:Petroleum; D012989:Soil Pollutants