title: Resveratrol Inhibits Proliferation and Induces Apoptosis of Pathological Scar Fibroblasts Through the Mechanism Involving TGF-β1/Smads Signaling Pathway.
abstract: The objective of this study was to determine the effect of resveratrol (Res) treatment on pathological scar fibroblasts and the changes in TGF-β1/Smads signaling pathway. For this purpose, cultured pathological scar fibroblasts were treated with various concentrations of Res (10, 50, and, 100 µmol/l), and the morphological changes in target cells were studied using scanning electron microscopy (SEM). The cellular proliferation was assessed by MTT assay; the mRNA and protein expressions of TGF-β1 and Smad-2,3,4,7 were determined by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence assay, respectively. We found that Res-treated fibroblasts exhibited the typical apoptotic morphological changes. As shown by MTT assay, the OD values of Res-treated fibroblasts, as a measure of cell growth, were significantly lower than those of controls (P < 0.05). In addition, as compared to controls, TGF-β1 and Smad-2,3,4 mRNA/protein expression decreased but those of Smad7 increased in a dose-dependent manner (P < 0.05). It was, therefore, concluded that Res treatment inhibited the pathological scar fibroblast proliferation and induced cell apoptosis through the mechanism involving downregulation of TGF-β1, Smad-2,3,4, and upregulation of Smad7.
authors.affiliation: Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, 221003, China.
authors.forename: Xiao-xiang
authors.initials: XX
authors.lastname: Zhai
authors.affiliation: Department of Plastic Surgery, Xuzhou Central Hospital, Xuzhou, 221009, China. djicun@126.com.
authors.forename: Ji-cun
authors.initials: JC
authors.lastname: Ding
authors.affiliation: Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, 221003, China.
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051785:Smad Proteins; D013267:Stilbenes; D053773:Transforming Growth Factor beta1; D000077185:Resveratrol
keywords: Fibroblasts; Pathological scar; Resveratrol; TGF-β1/Smads signaling pathway