title: Metabolic differences between white and brown fat from fasting rabbits at physiological temperature.
abstract: It has been suggested that activated brown adipose tissue (BAT) shows increased glucose metabolic activity. However, less is known about metabolic activity of BAT under conditions of fasting and normal temperature. The aim of this study was to compare the possible differences in energetic metabolism between BAT and white adipose tissue (WAT) obtained from rabbits under the conditions of physiological temperature and 24 h after fasting conditions. The study was carried out on New Zealand rabbits (n=10) maintained for a period of 8 weeks at 23±2 °C. Food was removed 24 h before BAT and WAT were obtained. Protein expression levels of the glycolytic-related protein, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate dehydrogenase were higher in WAT than that in BAT. The expression level of carnitine palmitoyltransferase 1 (CPT1) and CPT2, two fatty acid mitochondrial transporters, and the fatty acid β-oxidation-related enzyme, acyl CoA dehydrogenase, was higher in BAT than in WAT. Cytosolic malate dehydrogenase expression and malate dehydrogenase activity were higher in WAT than in BAT. However, lactate dehydrogenase expression and lactate content were significantly higher in BAT than in WAT. In summary, this study for the first time, to our knowledge, has described how under fasting and normal temperature conditions rabbit BAT seems to use anaerobic metabolism to provide energetic fuel, as opposed to WAT, where the malate-aspartate shuttle and, therefore, the gluconeogenic pathway seem to be potentiated.
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: Z
authors.initials: Z
authors.lastname: López-Ibarra
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Modrego
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Valero-Muñoz
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Rodríguez-Sierra
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zamorano-León
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: A
authors.initials: A
authors.lastname: González-Cantalapiedra
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: N
authors.initials: N
authors.lastname: de Las Heras
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ballesteros
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain ajlf@telefonica.net vlahera@med.ucm.es.
authors.forename: V
authors.initials: V
authors.lastname: Lahera
authors.affiliation: Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain Surgery DepartmentHospital Universitario ROF-Codina, Lugo, SpainInstituto de Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC)Madrid, SpainDepartments of PhysiologyMedicineSchool of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain ajlf@telefonica.net vlahera@med.ucm.es.
authors.forename: A J
authors.initials: AJ
authors.lastname: López-Farré
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000201:enzymology / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D011817:Rabbits; D013696:Temperature
chemical_list: D005227:Fatty Acids; D024101:Mitochondrial Proteins; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D000154:Aconitate Hydratase; D005947:Glucose
keywords: adipose tissue; energetic metabolism; gluconeogenesis