title: CXCL9 and CXCL10 gene polymorphisms in patients with rheumatoid arthritis.
abstract: Chemokines (CXCL) and their receptors play important roles in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to examine the associations between polymorphisms in the CXCL9 (rs3733236 G>A) and CXCL10 (rs8878 A>G) genes and RA. We examined 422 RA patients and 338 subjects as a control group. Single nucleotide polymorphisms (SNPs) in the CXCL9 (rs3733236 G>A) and CXCL10 (rs8878 A>G) genes were genotyped using TaqMan genotyping assays from Life Technologies Genomic. There were no significant differences in distribution of CXCL9 genotypes and alleles between RA patients and control group. Among RA patients, the increased frequency of CXCL10 (rs8878) G allele carriers was detected AG+GG vs AA (p = 0.034; OR 1.49, 95 % CI 1.03-2.13). There were no significant associations of CXCL9 genotypes with age of disease diagnosis rheumatoid factor, erosive disease, and extra-articular manifestations. In case of CXCL10 genotypes, there was the increased frequency of extra-articular manifestations in GG genotype carriers GG vs AA+AG (p = 0.027; OR 1.82, 95 % CI 1.09-3.03). In the multivariate regression analysis, the CXCL10 GG genotype was the independent factor associated with increased probability of extra-articular manifestations development (p = 0.034; OR 1.75, 95 % CI 1.04-3.03). The results of this study suggest the association between CXCL10 gene polymorphism and rheumatoid arthritis.
authors.affiliation: Departament of Orthopaedics, Traumatology and Orthopaedic Oncology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kotrych
authors.forename: Violetta
authors.initials: V
authors.lastname: Dziedziejko
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Marek
authors.initials: M
authors.lastname: Drozdzik
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pawlik
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000235:genetics*; D054370:Chemokine CXCL9 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide; D012044:Regression Analysis; D012217:Rheumatoid Factor / Q000276:immunology; D012720:Severity of Illness Index
chemical_list: C114753:CXCL10 protein, human; C080810:CXCL9 protein, human; D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; D012217:Rheumatoid Factor