title: The effect of combined use of platelet-rich plasma and adipose-derived stem cells on fat graft survival.
abstract: BACKGROUND
  Free fat grafts have an unpredictable survival rate that limits their successful use. To increase the viability of fat grafts, it is important to minimize the reabsorption rate.
  
  
  OBJECTIVE
  Our aim was to investigate whether the combined use of platelet-rich plasma (PRP) and adipose derived stem cells (ADSCs) would contribute an improvement in lower resorption rates of fat grafts.
  
  
  METHODS
  Inbred Fischer 344 rats were randomized into 4 groups (n = 10). Fat grafts were mixed with Dulbecco modified Eagle medium in group A, with PRP in group B, with ADSC in group C, and with PRP + ADSC in group D and were injected to the scalp.In vitro growth factor (vascular endothelial growth factor, transforming growth factor-β, and fibroblast growth factor) levels were compared using enzyme-linked immunoassay method. After 12 weeks weight, volume and histology of the transplants were evaluated.
  
  
  RESULTS
  The mean weight and volume of the fat grafts were highest in group D. Histopathological investigations revealed that the number of viable adipocytes and blood vessels were highest in group D. The level of growth factors was significantly higher in stem cell plus PRP group.
  
  
  CONCLUSIONS
  Adipose-derived stem cells combined with PRP can enhance the survival of transplanted fat tissue.
authors.affiliation: From the *Department of Plastic and Reconstructive Surgery, Meram Medical School,Necmettin Erbakan University, Konya; †Department of Plastic and Reconstructive Surgery, ‡Department of Hematology, §Department of Pathology, Gulhane Military Academy, Ankara; and ∥Department of Pathology, Meram MedicalSchool, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Nevra
authors.initials: N
authors.lastname: Seyhan
authors.forename: Dogan
authors.initials: D
authors.lastname: Alhan
authors.forename: Ali Ugur
authors.initials: AU
authors.lastname: Ural
authors.forename: Armagan
authors.initials: A
authors.lastname: Gunal
authors.forename: Mustafa Cihad
authors.initials: MC
authors.lastname: Avunduk
authors.forename: Nedim
authors.initials: N
authors.lastname: Savaci
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D006085:Graft Survival; D045164:Mesenchymal Stem Cell Transplantation; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344