title: Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats.
abstract: OBJECTIVE
  To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod immunoexpression in a model of dorsal burn in rats.
  
  
  METHODS
  Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry.
  
  
  RESULTS
  The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher.
  
  
  CONCLUSIONS
  Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.
authors.affiliation: Institutional Program for Scientific Initiation, National Council of Scientific and Technological Development, Ministry of Science, Technology and Inovation, Brazil.
authors.forename: Francielle
authors.initials: F
authors.lastname: Martins
authors.affiliation: Bioscience Department, UNIFESP, Santos, SP, Brazil.
authors.forename: Ana Cláudia Muniz
authors.initials: AC
authors.lastname: Rennó
authors.affiliation: Bioscience Department, UNIFESP, Santos, SP, Brazil.
authors.forename: Flávia de
authors.initials: Fd
authors.lastname: Oliveira
authors.affiliation: School of Medicine, UNIFESP, Santos, SP, Brazil.
authors.forename: Natália Peruchi
authors.initials: NP
authors.lastname: Minatel
authors.affiliation: Postgraduate Program in Interdisciplinary Health Sciences, UNIFESP, Santos, SP, Brazil.
authors.forename: Jeferson André
authors.initials: JA
authors.lastname: Bortolin
authors.affiliation: Postgraduate Program in Interdisciplinary Health Sciences, UNIFESP, Santos, SP, Brazil.
authors.forename: Hananiah Tardivo
authors.initials: HT
authors.lastname: Quintana
authors.affiliation: Department of Human Movement Sciences, UNIFESP, Santos, SP, Brazil.
authors.forename: Mariana Chaves
authors.initials: MC
authors.lastname: Aveiro
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D002452:Cell Count; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000528:radiation effects; D018482:Muscle, Skeletal / Q000473:pathology / Q000528:radiation effects*; D017570:MyoD Protein / Q000032:analysis* / Q000528:radiation effects; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000293:injuries / Q000528:radiation effects; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D017570:MyoD Protein