title: Correlation between sperm parameters and protein expression of antioxidative defense enzymes in seminal plasma: a pilot study.
abstract: BACKGROUND
  Semen analysis is the cornerstone in the evaluation of male (in)fertility. However, there are men with normal semen tests but with impaired fertilizing ability, as well as fertile men with poor sperm characteristics. Thus, there is rising interest to find novel parameters that will help to predict and define the functional capacity of spermatozoa.
  
  
  METHODS
  We examined whether there is a correlation between semen parameters (count, progressive motility, and morphology) and protein expression/activity of antioxidative defense enzymes in seminal plasma from 10 normospermic subjects.
  
  
  RESULTS
  Sperm progressive motility was in positive correlation with seminal plasma protein expression of both superoxide dismutase (SOD) isoforms (MnSOD and CuZnSOD) and catalase. Also, positive correlation was observed between sperm count and MnSOD protein expression, as well as between sperm morphology and protein expression of catalase in seminal plasma. In contrast, protein expression of glutathione peroxidase was not in correlation with any sperm parameter, while its activity negatively correlated with sperm morphology and motility.
  
  
  CONCLUSIONS
  These data suggest that evaluation of protein expression of antioxidative defense enzymes in seminal plasma might be of importance in the evaluation of male fertility status and that could be used as an additional biomarker along with classic semen analysis in assessment of semen quality.
authors.affiliation: The Clinic of Gynecology and Obstetrics "Narodni front", 11000 Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Macanovic
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Vucetic
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jankovic
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Ana
authors.identifier: 0000-0003-0806-0799
authors.initials: A
authors.lastname: Stancic
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Buzadzic
authors.affiliation: The Clinic of Gynecology and Obstetrics "Narodni front", 11000 Belgrade, Serbia.
authors.forename: Eliana
authors.initials: E
authors.lastname: Garalejic
authors.affiliation: Institute of Zoology and Center for Electron Microscopy, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.identifier: 0000-0002-3044-9963
authors.initials: A
authors.lastname: Korac
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Bato
authors.identifier: 0000-0001-5272-579X
authors.initials: B
authors.lastname: Korac
authors.affiliation: The Clinic of Gynecology and Obstetrics "Narodni front", 11000 Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Otasevic
mesh_terms: D000328:Adult; D002374:Catalase / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008297:Male; D010865:Pilot Projects; D020033:Protein Isoforms / Q000378:metabolism; D012661:Semen / Q000201:enzymology* / Q000502:physiology; D055101:Semen Analysis; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase