title: [Hydrolysis of chitozan with an enzyme complex from Myceliophthora sp].
abstract: Samples of low-molecular weight chitosan with molecular masses of 8-24 kDa, identical deacetylation degrees (85%), and polydispersity indexes soluble at pH 5-7 were obtained by enzymatic hydrolysis using an enzyme complex from the micelial fungi Myceliophthora fergusii with yields of 50-80%. The optimal conditions for hydrolysis were found (pH 5.6, 37 degrees C, an enzyme/substrate ratio 1/800, 15-60 min). The obtainment of chitosan sample sets with different characteristics will enable the selection of the most efficient ones for comparison in in vitro/in vivo experiments.
authors.forename: L M
authors.initials: LM
authors.lastname: Khasanova
authors.forename: A V
authors.initials: AV
authors.lastname: Il'ina
authors.forename: V P
authors.initials: VP
authors.lastname: Varlamov
authors.forename: O A
authors.initials: OA
authors.lastname: Sinitsyna
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D002688:Chitinases / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D005612:Freeze Drying; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry* / Q000201:enzymology; D004718:Saccharomycetales / Q000737:chemistry* / Q000201:enzymology; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D048271:Chitosan; D002688:Chitinases