title: Regulation of ascorbic acid metabolism by blue LED light irradiation in citrus juice sacs.
abstract: In the present study, the effects of red and blue LED lights on the accumulation of ascorbic acid (AsA) were investigated in the juice sacs of three citrus varieties, Satsuma mandarin, Valencia orange, and Lisbon lemon. The results showed that the blue LED light treatment effectively increased the AsA content in the juice sacs of the three citrus varieties, whereas the red LED light treatment did not. By increasing the blue LED light intensity, the juice sacs of the three citrus varieties accumulated more AsA. Moreover, continuous irradiation with blue LED light was more effective than pulsed irradiation for increasing the AsA content in the juice sacs of the three citrus varieties. Gene expression results showed that the modulation of AsA accumulation by blue LED light was highly regulated at the transcription level. The up-regulation of AsA biosynthetic genes (CitVTC1, CitVTC2, CitVTC4, and CitGLDH), AsA regeneration genes (CitMDAR1, CitMDAR2, and CitDHAR) and two GSH-producing genes (CitGR and CitchGR) contributed to these increases in the AsA content in the three citrus varieties.
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Lancui
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamawaki
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikoma
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Terutaka
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohta
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan. Electronic address: amkato@ipc.shizuoka.ac.jp.
authors.forename: Masaya
authors.initials: M
authors.lastname: Kato
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D002957:Citrus / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D003116:Color; D018506:Gene Expression Regulation, Plant; D008027:Light; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D001205:Ascorbic Acid
keywords: AsA; Continuous irradiation; Pulsed irradiation; Regulatory mechanism