title: Response of α-glucosidase in gypsy moth larvae to acute and chronic dietary cadmium.
abstract: We investigated the effects of acute and chronic treatments with cadmium at 10 μg Cd/g dry food and 30 μg Cd/g dry food on α-glucosidase activity of the 4th instar larvae of Lymantria dispar (Lepidoptera: Lymantriidae) as well as subsequent recovery. Enzyme inhibition was recorded during acute exposure to 30 μg Cd/g dry food and during chronic treatment at the lower metal concentration. After three days recovery from 10 μg Cd/g dry food, the α-glucosidase activity returned to the control level. One-way ANOVA showed that cadmium significantly influenced the activity of α-glucosidase during all treatments. The index of phenotypic plasticity was higher during chronic treatment at 10 μg Cd/g dry food than at 30 μg Cd/g as well as during the recovery. We detected four glucosidase isoforms by NATIVE PAGE. The activities and expressions of the isoforms depended on both larval genotype and cadmium treatment.
authors.affiliation: a Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković," University of Belgrade , Belgrade , Serbia.
authors.forename: Milena S
authors.initials: MS
authors.lastname: Vlahović
authors.forename: Vesna D Perić
authors.initials: VD
authors.lastname: Mataruga
authors.forename: Jelica M
authors.initials: JM
authors.lastname: Lazarević
authors.forename: Marija M
authors.initials: MM
authors.lastname: Mrdaković
authors.forename: Dragana R
authors.initials: DR
authors.lastname: Matić
authors.forename: Dajana D
authors.initials: DD
authors.lastname: Todorović
authors.forename: Larisa A
authors.initials: LA
authors.lastname: Ilijin
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004789:Enzyme Activation / Q000187:drug effects; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects* / Q000201:enzymology; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D019476:Insect Proteins; D002104:Cadmium; D000520:alpha-Glucosidases
keywords: Cadmium; digestive enzyme; enzyme isoforms; gypsy moth; phenotypic plasticity