title: Enhanced resolution through thick tissue with structured illumination and adaptive optics.
abstract: Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 μm of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO.
authors.affiliation: University of Georgia, College of Engineering, 101 Driftmier Engineering Center, Athens, Georgia 30602, United States.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thomas
authors.affiliation: University of Georgia, College of Veterinary Medicine, Department of Infectious Diseases, Athens, Georgia 30602, United States.
authors.forename: Adrian
authors.initials: A
authors.lastname: Wolstenholme
authors.affiliation: University of Georgia, Department of Cellular Biology, 724 Biological Sciences Building, Athens, Georgia 30602, United States.
authors.forename: Snehal N
authors.initials: SN
authors.lastname: Chaudhari
authors.affiliation: University of Georgia, Department of Cellular Biology, 724 Biological Sciences Building, Athens, Georgia 30602, United States.
authors.forename: Edward T
authors.initials: ET
authors.lastname: Kipreos
authors.affiliation: University of Georgia, College of Engineering, 101 Driftmier Engineering Center, Athens, Georgia 30602, United States.
authors.forename: Peter
authors.initials: P
authors.lastname: Kner
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D061848:Optical Imaging / Q000379:methods*; D059629:Signal-To-Noise Ratio