title: Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae.
abstract: Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with an LD50 of about 2.3 × 10(3) c.f.u. ml(-1) after 24 h. Moreover, complete or gel permeation HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECP fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease.
authors.affiliation: Fédération de Recherche en Environnement et Développement Durable, FR CNRS 3097, Université de La Rochelle, La Rochelle, France.|IFREMER, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins Avenue de Mus de Loup, 17390 La Tremblade, France.|UMR 7266 CNRS-ULR, LIENSs, Equipe Approches Moléculaires, Environnement-Santé, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Rachida
authors.initials: R
authors.lastname: Mersni-Achour
authors.affiliation: UMR 7266 CNRS-ULR, LIENSs, Equipe Approches Moléculaires, Environnement-Santé, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Yosra Ben
authors.initials: YB
authors.lastname: Cheikh
authors.affiliation: Laboratoire des Sciences de l'Environnement Marin LEMAR, UMR 6539 CNRS/UBO/IRD/IFREMER, Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Université Européenne de Bretagne, 29280 Plouzané, France.
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
authors.affiliation: UMR 7266 CNRS-ULR, LIENSs, Equipe Approches Moléculaires, Environnement-Santé, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Doghri
authors.affiliation: UMR 7266 CNRS-ULR, LIENSs, Equipe Approches Moléculaires, Environnement-Santé, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Etien
authors.affiliation: IFREMER, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Dégremont
authors.affiliation: IFREMER, Centre Ifremer du Pacifique, UMR 241 Ecosystèmes Insulaires Océaniens, Tahiti, 98719 Taravao, French Polynesia.|IFREMER, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Saulnier
authors.affiliation: Fédération de Recherche en Environnement et Développement Durable, FR CNRS 3097, Université de La Rochelle, La Rochelle, France.|UMR 7266 CNRS-ULR, LIENSs, Equipe Approches Moléculaires, Environnement-Santé, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Fruitier-Arnaudin
authors.affiliation: IFREMER, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Travers
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015870:Gene Expression; D007814:Larva / Q000382:microbiology; D045726:Metalloproteases / Q000235:genetics* / Q000378:metabolism; D010125:Ostreidae / Q000382:microbiology*; D010641:Phenotype; D014733:Vibrio / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D045726:Metalloproteases