title: Characterization of lipid films by an angle-interrogation surface plasmon resonance imaging device.
abstract: Surface topographies of lipid films have an important significance in the analysis of the preparation of giant unilamellar vesicles (GUVs). In order to achieve accurately high-throughput and rapidly analysis of surface topographies of lipid films, a homemade SPR imaging device is constructed based on the classical Kretschmann configuration and an angle interrogation manner. A mathematical model is developed to accurately describe the shift including the light path in different conditions and the change of the illumination point on the CCD camera, and thus a SPR curve for each sampling point can also be achieved, based on this calculation method. The experiment results show that the topographies of lipid films formed in distinct experimental conditions can be accurately characterized, and the measuring resolution of the thickness lipid film may reach 0.05 nm. Compared with existing SPRi devices, which realize detection by monitoring the change of the reflective-light intensity, this new SPRi system can achieve the change of the resonance angle on the entire sensing surface. Thus, it has higher detection accuracy as the traditional angle-interrogation SPR sensor, with much wider detectable range of refractive index.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Chongqing City Management College, Chongqing 400055, China.
authors.forename: Wangang
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China. Electronic address: huning@cqu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China.
authors.forename: Yanjian
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China. Electronic address: bioyangjun@cqu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D004867:Equipment Design; D008027:Light; D008432:Mathematical Computing; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry*; D012031:Refractometry; D020349:Surface Plasmon Resonance; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes
keywords: Angle interrogation; Biosensor; Lipid film; Mathematical model; Surface plasmon resonance