title: Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of β-cyclodextrin on the lipid-probe complexes: a fluorometric investigation.
abstract: Interaction of cationic phenosafranin (PSF), anionic 8-anilino-1-naphthalene sulfonate (ANS) and non-ionic nile red (NR) have been studied with the zwitterionic phospholipid, egg yolk L-α-phosphatidylcholine (EYPC). The study reveals discernible binding interactions of the three fluorescent probes with the EYPC lipid vesicle. Once the binding of the probes with the lipid is established, the effect of cyclic oligosaccharide, β-cyclodextrin (β-CD), on these lipid bound probes has been investigated. Different fluorometric techniques suggest that addition of β-CD to the probe-lipid complexes leads to the release of the probes from the lipid medium through the formation of probe-β-CD inclusion complexes. A competitive binding of the probes between β-cyclodextrin and the lipid is ascribed to be responsible for the effect. This provides an easy avenue for the removal of the probe molecules from the lipid environment. Extension of this work with drug molecules in cell membranes is expected to give rise to a strategy for the removal of adsorbed drugs from the cell membranes by the use of non-toxic β-cyclodextrin.
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Pronab
authors.initials: P
authors.lastname: Kundu
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Saptarshi
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Barnali
authors.initials: B
authors.lastname: Jana
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India. Electronic address: nitin.chattopadhyay@yahoo.com.
authors.forename: Nitin
authors.initials: N
authors.lastname: Chattopadhyay
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D004530:Egg Yolk / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D010078:Oxazines / Q000737:chemistry; D010619:Phenazines / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: C515594:8-anilino-1-naphthalenesulfonic acid; D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D010078:Oxazines; D010619:Phenazines; D010713:Phosphatidylcholines; D047392:beta-Cyclodextrins; C011977:phenosafranine; C031215:betadex; C044808:nile red
keywords: Competitive binding; Fluorescence; Inclusion complex; Phospholipid; β-Cyclodextrin