title: [Investigation of mechanisms of interaction between inulinase from Kluyveromyces marxianus and the matrices of ion-exchange resins and fiber].
abstract: It is established that ion exchange resins AV-17-2P, KU-2, AV-16-GS, AM 21A, IMAC-HP, PUROLITE and fiber VION KN-1 can be applied as carriers for inulinase immobilization. The analysis of IR spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through electrostatic interactions. It is assumed that the mechanisms of interaction between inulinase from Kluyveromyces marxianus and the matrices of cation- and anion exchange polymers differ essentially from each other: different sites of protein molecule take part in adsorption that causes various conformational reorganizations in an enzyme molecule.
authors.forename: M G
authors.initials: MG
authors.lastname: Holyavka
authors.forename: T A
authors.initials: TA
authors.lastname: Kovaleva
authors.forename: S I
authors.initials: SI
authors.lastname: Karpov
authors.forename: P V
authors.initials: PV
authors.lastname: Seredin
authors.forename: V G
authors.initials: VG
authors.lastname: Artyukhov
mesh_terms: D065966:Absorption, Physicochemical; D005656:Fungal Proteins / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D007475:Ion Exchange Resins / Q000737:chemistry*; D007716:Kluyveromyces / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D007475:Ion Exchange Resins; D006026:Glycoside Hydrolases; C023086:inulinase