title: Amplification of the signal intensity of fluorescence-based fiber-optic biosensors using a Fabry-Perot resonator structure.
abstract: Fluorescent biosensors have been widely used in biomedical applications. To amplify the intensity of fluorescence signals, this study developed a novel structure for an evanescent wave fiber-optic biosensor by using a Fabry-Perot resonator structure. An excitation light was coupled into the optical fiber through a laser-drilled hole on the proximal end of the resonator. After entering the resonator, the excitation light was reflected back and forth inside the resonator, thereby amplifying the intensity of the light in the fiber. Subsequently, the light was used to excite the fluorescent molecules in the reactive region of the sensor. The experimental results showed that the biosensor signal was amplified eight-fold when the resonator reflector was formed using a 92% reflective coating. Furthermore, in a simulation, the biosensor signal could be amplified 20-fold by using a 99% reflector.
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, 1 Sec.4 Roosevelt Road, Taipei 10617, Taiwan. yihsinchiu07@gmail.com.|Institute of Biomedical Engineering, National Yang-Ming University, No.155, Sec.2 Linong Street, Taipei 11221, Taiwan. yihsinchiu07@gmail.com.
authors.forename: Meng-Chang
authors.initials: MC
authors.lastname: Hsieh
authors.affiliation: Institute of Biomedical Engineering, National Yang-Ming University, No.155, Sec.2 Linong Street, Taipei 11221, Taiwan. yihsinchiu07@gmail.com.
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Chiu
authors.affiliation: Department of Optics and Photonics, National Central University, Jhongli 32001, Taiwan. 982406004@cc.ncu.edu.tw.
authors.forename: Sheng-Fu
authors.initials: SF
authors.lastname: Lin
authors.affiliation: Department of Optics and Photonics, National Central University, Jhongli 32001, Taiwan. jychang@dop.ncu.edu.tw.
authors.forename: Jenq-Yang
authors.initials: JY
authors.lastname: Chang
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, 1 Sec.4 Roosevelt Road, Taipei 10617, Taiwan. changco@iam.ntu.edu.tw.
authors.forename: Chia-Ou
authors.initials: CO
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Engineering, National Yang-Ming University, No.155, Sec.2 Linong Street, Taipei 11221, Taiwan. hkchiang@ym.edu.tw.
authors.forename: Huihua Kenny
authors.initials: HK
authors.lastname: Chiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005336:Fiber Optic Technology / Q000379:methods*; D005453:Fluorescence