title: Microbiological and clinical aspects of respiratory infections associated with Bordetella bronchiseptica.
abstract: Bordetella bronchiseptica is a well-known veterinary pathogen, but its implication in human disease is probably not fully recognized. The purpose of this study was to determine the clinical significance of 36 B. bronchiseptica isolates from respiratory samples of 22 patients. Therefore, we describe microbiological characteristics, including phenotypic and genotypic identification as well as antimicrobial susceptibilities of the isolates. Clonal relatedness was evaluated using pulsed-field gel electrophoresis (PFGE). Most of the patients had some underlying immunosuppressive condition. Eighteen out of 22 (82%) patients had respiratory symptoms, and the death of 2 patients was associated with respiratory infection.All strains were correctly identified at species level by the simultaneous use of phenotypic methods and were confirmed by specific amplification of the upstream region of the fla gene. Tigecycline, minocycline, doxycycline, colistin, and meropenem were the most active agents tested. PFGE analysis revealed that repeated infections involving each patient had been caused by the same strain.
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain. Electronic address: cgarcia@humv.es.
authors.forename: Celia
authors.initials: C
authors.lastname: García-de-la-Fuente
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Guzmán
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
authors.forename: María Eliecer
authors.initials: ME
authors.lastname: Cano
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain; Department of Molecular Biology, School of Medicine, University of Cantabria, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Agüero
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sanjuán
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodríguez
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Aguirre
authors.affiliation: Service of Microbiology, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain; Department of Molecular Biology, School of Medicine, University of Cantabria, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001885:Bordetella Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology*; D016950:Bordetella bronchiseptica / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D010641:Phenotype; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: Bordetella bronchiseptica; Molecular identification; Pulsed-field gel electrophoresis; Respiratory infections; Veterinary pathogen