title: Regulation of primary metabolic pathways in oyster mushroom mycelia induced by blue light stimulation: accumulation of shikimic acid.
abstract: Shikimic acid is a key intermediate in the aromatic amino acid pathway as well as an important starting material for the synthesis of Tamiflu, a potent and selective inhibitor of the neuraminidase enzyme of influenza viruses A and B. Here we report that in oyster mushroom (Pleurotus ostreatus) mycelia cultivated in the dark, stimulation with blue light-emitting diodes induces the accumulation of shikimic acid. An integrated analysis of primary metabolites, gene expression and protein expression suggests that the accumulation of shikimic acid caused by blue light stimulation is due to an increase in 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (DAHPS, EC2.5.1.54), the rate-determining enzyme in the shikimic acid pathway, as well as phosphofructokinase (PFK, EC2.7.1.11) and glucose-6-phosphate dehydrogenase (G6PD, EC1.1.1.49), the rate-determining enzymes in the glycolysis and pentose phosphate pathways, respectively. This stimulation results in increased levels of phosphoenolpyruvic acid (PEP) and erythrose-4-phosphate (E4P), the starting materials of shikimic acid biosynthesis.
authors.affiliation: Department of Bioscience and Biotechnology, Shinshu University, Minamiminowa, Kamiina, Nagano, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kojima
authors.affiliation: Department of Bioscience and Biotechnology, Shinshu University, Minamiminowa, Kamiina, Nagano, Japan.
authors.forename: Ninako
authors.initials: N
authors.lastname: Kimura
authors.affiliation: Department of Bioscience and Biotechnology, Shinshu University, Minamiminowa, Kamiina, Nagano, Japan.
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Miura
mesh_terms: D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D006019:Glycolysis; D008027:Light; D053858:Metabolic Networks and Pathways / Q000528:radiation effects*; D025282:Mycelium / Q000378:metabolism* / Q000528:radiation effects; D020076:Pleurotus / Q000378:metabolism* / Q000528:radiation effects; D012765:Shikimic Acid / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D012765:Shikimic Acid