title: Infectious causes of cholesteatoma and treatment of infected ossicles prior to reimplantation by hydrostatic high-pressure inactivation.
abstract: Chronic inflammation, which is caused by recurrent infections, is one of the factors contributing to the pathogenesis of cholesteatoma. If reimplantation of autologous ossicles after a surgical intervention is intended, inactivation of planktonic bacteria and biofilms is desirable. High hydrostatic pressure treatment is a procedure, which has been used to inactivate cholesteatoma cells on ossicles. Here we discuss the potential inactivating effect of high hydrostatic pressure on microbial pathogens including biofilms. Recent experimental data suggest an incomplete inactivation at a pressure level, which is tolerable for the bone substance of ossicles and results at least in a considerable reduction of pathogen load. Further studies are necessary to access how far this quantitative reduction of pathogens is sufficient to prevent ongoing chronic infections, for example, due to forming of biofilms.
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen, 37075 Göttingen, Germany.
authors.forename: Wycliffe Omurwa
authors.identifier: 0000-0003-4194-9055
authors.initials: WO
authors.lastname: Masanta
authors.affiliation: Fachbereich Tropenmedizin am Bernhard-Nocht-Institut, Bundeswehrkrankenhaus Hamburg, 20359 Hamburg, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hinz
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen, 37075 Göttingen, Germany.
authors.forename: Andreas Erich
authors.identifier: 0000-0003-0984-013X
authors.initials: AE
authors.lastname: Zautner
mesh_terms: D001419:Bacteria / Q000145:classification / Q000472:pathogenicity / Q000528:radiation effects; D018441:Biofilms / Q000254:growth & development / Q000528:radiation effects; D002781:Cholesteatoma / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D004429:Ear Ossicles / Q000382:microbiology / Q000473:pathology / Q000528:radiation effects; D005658:Fungi / Q000145:classification / Q000472:pathogenicity / Q000528:radiation effects; D006801:Humans; D006874:Hydrostatic Pressure; D007249:Inflammation / Q000150:complications / Q000382:microbiology / Q000473:pathology* / Q000628:therapy*