title: Polymorphic regions in the interleukin-1 gene and susceptibility to chronic periodontitis: a genetic association study.
abstract: OBJECTIVE
  The objectives of this study were to determine the association between single nucleotide polymorphisms (SNPs) in IL1B (-511, +3954), IL1A (-889, +4845), and the variable number of tandem repeats (VNTRs) polymorphism in the IL-1RN gene with chronic periodontitis susceptibility and to analyze gene-gene interactions in a hospital-based sample population from South India.
  
  
  METHODS
  A total of 400 individuals were recruited for this study; 200 individuals with healthy gingiva and 200 chronic periodontitis patients. Genomic DNA was isolated from peripheral blood samples and genotyping was performed for the above-mentioned single nucleotide and VNTR polymorphisms by polymerase chain reaction, DNA sequencing, and agarose gel electrophoresis.
  
  
  RESULTS
  A higher proportion of the variant alleles were observed in the chronic periodontitis group for all the SNPs examined. The SNP at +3954 (C>T) in the IL1B gene was found to be significantly associated with chronic periodontitis (p=0.007). VNTR genotypes (χ(2) value: 5.163, df=1, p=0.023) and alleles (χ(2) value: 6.818, df=1, p=0.009) were found to have a significant association with chronic periodontitis susceptibility.
  
  
  CONCLUSIONS
  In the study population examined, the SNP in the IL1B gene (+3954) and VNTR polymorphisms in the IL1RN gene were found to have a significant association with chronic periodontitis susceptibility.
authors.affiliation: 1 Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors.forename: Vamsi
authors.initials: V
authors.lastname: Lavu
authors.forename: Vettriselvi
authors.initials: V
authors.lastname: Venkatesan
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Venkata Kameswara Subrahmanya Lakkakula
authors.forename: Priyanka
authors.initials: P
authors.lastname: Venugopal
authors.forename: Solomon Franklin Durairaj
authors.initials: SF
authors.lastname: Paul
authors.forename: Suresh Ranga
authors.initials: SR
authors.lastname: Rao
mesh_terms: D000328:Adult; D000483:Alleles; D055113:Chronic Periodontitis / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India / Q000453:epidemiology; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: C508585:IL1B protein, human; D053583:Interleukin-1beta