title: A rapid liquid chromatography determination of free formaldehyde in cod.
abstract: A rapid method for the determination of free formaldehyde in cod is described. It uses a simple water extraction of formaldehyde which is then derivatised with 2,4-dinitrophenylhydrazine (DNPH) to form a sensitive and specific chromophore for high-performance liquid chromatography (HPLC) detection. Although this formaldehyde derivative has been widely used in past tissue analysis, this paper describes an improved derivatisation procedure. The formation of the DNPH formaldehyde derivative has been shortened to 2 min and a stabilising buffer has been added to the derivative to increase its stability. The average recovery of free formaldehyde in spiked cod was 63% with an RSD of 15% over the range of 25-200 mg kg(-1) (n = 48). The HPLC procedure described here was also compared to a commercial qualitative procedure - a swab test for the determination of free formaldehyde in fish. Several positive samples were compared by both methods.
authors.affiliation: a US Food and Drug Administration , Animal Drugs Research Center , Denver , CO 80225 , USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Storey
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Andersen
authors.forename: Andrea
authors.initials: A
authors.lastname: Heise
authors.forename: Sherri B
authors.initials: SB
authors.lastname: Turnipseed
authors.forename: Jack
authors.initials: J
authors.lastname: Lohne
authors.forename: Terri
authors.initials: T
authors.lastname: Thomas
authors.forename: Mark
authors.initials: M
authors.lastname: Madson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D005557:Formaldehyde / Q000032:analysis*; D048389:Gadus morhua; D010659:Phenylhydrazines / Q000032:analysis; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*
chemical_list: D010659:Phenylhydrazines; D005557:Formaldehyde; C004787:2,4-dinitrophenylhydrazine
keywords: 2,4-dinitrophenylhydrazine; cod; formaldehyde; liquid chromatography