title: Mass spectrometry-based method to investigate the natural selectivity of sucrose as the sugar transport form for plants.
abstract: Sucrose is the carbon skeletons and energy vector for plants, which is important for plants growth. Among thousands of disaccharides in Nature, why chose sucrose for plants? In this paper, we analyzed the intrinsic structural characteristics of four sucrose isomers with different glycosidic linkage by mass spectrometry (MS) technique. Our results show that sucrose has the most labile glycosidic bond compared with other three isomers, which is helpful for releasing glucose and fructose unit. Besides, sucrose has the most stable integral structure, which is hard to dehydrate and degrade into fragments through losing one or three even four-carbon units, just as its three isomers. In other words, sucrose is more easily holds an integral structure during the transport process, whenever it is necessary, and sucrose can be cleaved into glucose and fructose easily. Besides, we also investigate the internal relationship of sucrose with K(+) by tandem mass spectrometry and viscosity measurement. The related results have shown that the K(+) can stabilize sucrose to a greater extent than the Na(+). Furthermore, under the same conditions, K(+) ions reduce the viscosity of sucrose-water system much more than Na(+). These results suggest that K(+) is a better co-transporter for sucrose. Of course, the transport of sucrose in plants is a very complicated process, which is involved in many proteins. This paper directly accounts for the basic structure feature of sucrose, and the results discovered could provide the novel insight for the answer why Nature chose sucrose for plants.
authors.affiliation: Department of Chemical Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Chemical Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Yile
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Chemical Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: stacyliu@xmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Chemical Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005, China; Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: yfzhao@xmu.edu.cn.
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001692:Biological Transport; D002236:Carbohydrate Conformation; D013058:Mass Spectrometry / Q000379:methods*; D010944:Plants / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013395:Sucrose / Q000737:chemistry* / Q000378:metabolism; D014783:Viscosity
chemical_list: D013395:Sucrose; D012964:Sodium; D011188:Potassium
keywords: Glycosidic bond; Mass spectrometry; Potassium ions; Sucrose transport