title: Multicolor 3D super-resolution imaging by quantum dot stochastic optical reconstruction microscopy.
abstract: We demonstrate multicolor three-dimensional super-resolution imaging with quantum dots (QSTORM). By combining quantum dot asynchronous spectral blueing with stochastic optical reconstruction microscopy and adaptive optics, we achieve three-dimensional imaging with 24 nm lateral and 37 nm axial resolution. By pairing two short-pass filters with two appropriate quantum dots, we are able to image single blueing quantum dots on two channels simultaneously, enabling multicolor imaging with high photon counts.
authors.affiliation: College of Engineering, University of Georgia, Athens, Georgia 30602, United States.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Engineering, University of Georgia, Athens, Georgia 30602, United States.
authors.forename: Kayvan F
authors.initials: KF
authors.lastname: Tehrani
authors.affiliation: College of Engineering, University of Georgia, Athens, Georgia 30602, United States.
authors.forename: Peter
authors.initials: P
authors.lastname: Kner
mesh_terms: D003116:Color; D056945:Hep G2 Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D055620:Optical Phenomena; D045663:Quantum Dots; D013269:Stochastic Processes
keywords: fluorescence microscopy; quantum dots; stochastic optical reconstruction microscopy; super-resolution microscopy