title: Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro.
abstract: β-mercaptoethanol can induce adult adipose-derived stromal cells to rapidly and efficiently differentiate into typical neuron-like cells in vitro. Immunohistochemistry showed that neuron specific enolase and neurofilament-200 expression gradually increased with the extension of induction time, and peaked at 5 hours. By contrast, glial fibrillary acidic protein was negatively expressed at all time points. Induced cells possessed a typical Nissl body, apoptosis showing condensed chromatin in the nucleus, autophagosomes with a bilayered membrane and autolysosomes in the cytoplasm at 5 hours. TUNEL assay and immunohistochemistry and immunofluorescence demonstrated that apoptosis and caspase-3 expression increased and peaked at 8 hours. Immunohistochemistry and immunofluorescence showed that microtubuleassociated protein light chain 3 gradually increased with induction and reached a peak at 5 hours. These results indicate that autophagy played an important role in protecting cells during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro.
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Qiaoyu
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Hebei Union University, Tangshan 063000, Hebei Province, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
keywords: adult adipose-derived stromal cells; apoptosis; autophagy; differentiation; neural regeneration; neuron-like cells