title: Anti-glycation activities of phenolic constituents from Silybum marianum (Milk Thistle) flower in vitro and on human explants.
abstract: Glycation is an ageing reaction of naturally occurring sugars with dermal proteins, with clinical signs appearing in vivo around age 30, and increasing steadily/regularly with age. The suppleness of the dermis is affected by the formation of bridges between proteins and sugars (Maillard's reaction). The accumulation of advanced glycation end products (AGEs) in skin plays a very important role in skin ageing. Therefore, natural compounds or extracts that possess antiglycation activities may have great anti-ageing potential. In the present study, Silybum marianum flower extract (SMFE) was demonstrated to possess antiglycation activity. We found that SMFE inhibits glycation reaction between BSA and glucose. In addition, antiglycation activity of SMFE was confirmed in a human skin explants model. SMFE reduced Nε-(carboxymethyl) lysine (CML) expression, whereas SMFE stimulated fibrillin-1 expression compared to treatment with methyglyoxal. An active ingredient contributing to the observed activities was identified as silibinin. The antiglycation activity of silibinin was dose-dependent. The beneficial effects of silibinin may be applied to prevention or management of AGE-mediated pathologies, targeting in a pleiotropic and complementary way the biochemical and cellular bases of skin aging.
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biost@biospectrum.com.
authors.forename: Seoungwoo
authors.initials: S
authors.lastname: Shin
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biofk@biospectrum.com.
authors.forename: Jung-A
authors.initials: JA
authors.lastname: Lee
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biotq@biospectrum.com.
authors.forename: Minkyung
authors.initials: M
authors.lastname: Kim
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. biosd@biospectrum.com.
authors.forename: Hyunwoo
authors.initials: H
authors.lastname: Kum
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. bioso@biospectrum.com.
authors.forename: Eunsun
authors.initials: E
authors.lastname: Jung
authors.affiliation: Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea. pdh@biospectrum.com.
authors.forename: Deokhoon
authors.initials: D
authors.lastname: Park
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D005419:Flavonoids / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000378:metabolism; D008875:Middle Aged; D020944:Silybum marianum / Q000737:chemistry*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D000077385:Silybin; D012838:Silymarin / Q000378:metabolism; D012867:Skin / Q000187:drug effects*; D023422:Skin Irritancy Tests / Q000379:methods; D054392:Adipokines
chemical_list: D000975:Antioxidants; D000071838:Fibrillin-1; D000071837:Fibrillins; D005419:Flavonoids; D017127:Glycation End Products, Advanced; D008840:Microfilament Proteins; D010636:Phenols; D010936:Plant Extracts; D000077385:Silybin; D012838:Silymarin; C000605722:FBN1 protein, human; D054392:Adipokines