title: miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo.
abstract: Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Li-Yin
authors.initials: LY
authors.lastname: Yeh
authors.affiliation: Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.|Department of Dentistry, Taipei Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Chung-Ji
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.|Department of Dentistry, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Yong-Kie
authors.initials: YK
authors.lastname: Wong
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Christine
authors.initials: C
authors.lastname: Chang
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.|Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.|Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan.
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Lin
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.|Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.|Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan.
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Chang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015687:Cell Hypoxia / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D057809:HEK293 Cells; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D023041:Xenograft Model Antitumor Assays
chemical_list: C561021:MIRN372 microRNA, human; D035683:MicroRNAs; C118585:P62 protein, human; D016601:RNA-Binding Proteins; D017382:Reactive Oxygen Species; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human
keywords: carcinoma; hypoxia; invasion; miR-372; mouth