title: F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation.
abstract: It has been demonstrated previously that F-box protein Fbxl15 targets HECT-type E3 Smurf1 and forms a functionally active SCF complex for ubiquitination and proteasomal degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. Different from Fbxl15, Fbxo3 targets all the Nedd4 family members for their degradation, indicating that Fbxo3 plays an important role in controlling the stability of Nedd4. Taken together, we show that Smurf1 is an endogenous substrate of Fbxo3. Our study gains further insight into the novel role of Fbxo3 in BMP signaling.
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing 100730, China.
authors.forename: Dongnian
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Ultrasound, Peking University Shougang Hospital, No.9 Jinyuanzhuang Road, Shijingshan District, Beijing 100144, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Shu
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang, Dongcheng District, Beijing 100730, China. Electronic address: yutaozhan@263.net.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China. Electronic address: zhanglq@nic.bmi.ac.cn.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D044783:F-Box Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D060066:Protein Interaction Maps; D059748:Proteolysis; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D019485:Bone Morphogenetic Proteins; D044783:F-Box Proteins; D046988:Proteasome Endopeptidase Complex; D044767:Ubiquitin-Protein Ligases; C533903:FBXO3 protein, human; C498342:SMURF1 protein, human
keywords: Fbxo3; Proteasomal degradation; Protein ubiquitination; Smurf1; Ubiquitin ligase