title: Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet.
abstract: OBJECTIVE
  To investigate activation of brown adipose tissue (BAT) stimulated by medium-chain triglyceride (MCT).
  
  
  METHODS
  30 Male C57BL/6J obese mice induced by fed high fat diet (HFD) were divided into 2 groups, and fed another HFD with 2% MCT or long-chain triglyceride (LCT) respectively for 12 weeks. Body weight, blood biochemical variables, interscapular brown fat tissue (IBAT) mass, expressions of mRNA and protein of beta 3-adrenergic receptors (β3-AR), uncoupling protein-1 (UCP1), hormone sensitive lipase (HSL), protein kinase A (PKA), and adipose triglyceride lipase (ATGL) in IBAT were measured.
  
  
  RESULTS
  Significant decrease in body weight and body fat mass was observed in MCT group as compared with LCT group (P<0.05) after 12 weeks. Greater increases in IBAT mass was observed in MCT group than in LCT group (P<0.05). Blood TG, TC, LDL-C in MCT group were decreased significantly, meanwhile blood HDL-C, ratio of HDL-C/LDL-C and norepinephrine were increased markedly. Expressions of mRNA and protein of β3-AR, UCP1, PKA, HSL, ATGL in BAT were greater in MCT group than in LCT group (P<0.05).
  
  
  CONCLUSIONS
  Our results suggest that MCT stimulated the activation of BAT, possible via norepinephrine pathway, which might partially contribute to reduction of the body fat mass in obese mice fed high fat diet.
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xin Sheng
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Yu
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Rong Xin
authors.initials: RX
authors.lastname: Zhang
authors.affiliation: Peking University Health Science Center, Beijing 100191, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xue
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xue Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Chang Yong
authors.initials: CY
authors.lastname: Xue
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects*; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry / Q000494:pharmacology*; D000071256:Uncoupling Protein 1; D015431:Weight Loss
chemical_list: D004041:Dietary Fats; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014280:Triglycerides; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1
keywords: Beta 3-adrenergic receptor; Brown adipose tissue; Medium-chain triglyceride; Uncoupling protein-1