title: A highly sensitive DNA sensor for attomolar detection of the BRCA1 gene: signal amplification with gold nanoparticle clusters.
abstract: Single stranded DNA fragments were conjugated onto gold nanoparticles leading to the formation of gold nanoparticle clusters upon hybridization with complementary strands. These clusters were successfully implemented for signal amplification in an electrochemical DNA sensor based on a graphene substrate. The sensor exhibited excellent sensitivity and selectivity with a detection limit of 50 attomolar target DNA.
authors.affiliation: Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, India. sandhya@nitc.ac.in.
authors.forename: P Abdul
authors.initials: PA
authors.lastname: Rasheed
authors.forename: N
authors.initials: N
authors.lastname: Sandhyarani
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D019398:Genes, BRCA1; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization
chemical_list: D004277:DNA, Single-Stranded; D006046:Gold; D004247:DNA