title: In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb.
abstract: Juxtaglomerular neurons (JGNs) of the mammalian olfactory bulb are generated throughout life. Their integration into the preexisting neural network, their differentiation and survival therein depend on sensory activity, but when and how these adult-born cells acquire responsiveness to sensory stimuli remains unknown. In vivo two-photon imaging of retrovirally labelled adult-born JGNs reveals that ~90% of the cells arrive at the glomerular layer after day post injection (DPI) 7. After arrival, adult-born JGNs are still migrating, but at DPI 9, 52% of them have odour-evoked Ca(2+) signals. Their odourant sensitivity closely resembles that of the parent glomerulus and surrounding JGNs, and their spontaneous and odour-evoked spiking is similar to that of their resident neighbours. Our data reveal a remarkably rapid functional integration of adult-born cells into the preexisting neural network. The mature pattern of odour-evoked responses of these cells strongly contrasts with their molecular phenotype, which is typical of immature, migrating neuroblasts.
authors.affiliation: Institute of Physiology II, University of Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany.
authors.forename: Yury
authors.initials: Y
authors.lastname: Kovalchuk
authors.affiliation: Department of Physiology, Yale University, New Haven, Connecticut 06510, USA.
authors.forename: Ryota
authors.initials: R
authors.lastname: Homma
authors.affiliation: Institute of Physiology II, University of Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Institute of Physiology II, University of Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Maslyukov
authors.affiliation: Institute of Physiology II, University of Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Hermes
authors.affiliation: Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thestrup
authors.affiliation: Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Griesbeck
authors.affiliation: Helmholtz Zentrum München, Institute of Stem Cell Research, 85764 Neuherberg, Germany.
authors.forename: Jovica
authors.initials: J
authors.lastname: Ninkovic
authors.affiliation: 1] Department of Physiology, Yale University, New Haven, Connecticut 06510, USA [2] Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Cohen
authors.affiliation: Institute of Physiology II, University of Tübingen, Keplerstrasse 15, 72074 Tübingen, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Garaschuk
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D058953:Neural Stem Cells / Q000166:cytology; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology*; D018034:Olfactory Receptor Neurons / Q000166:cytology*; D010641:Phenotype; D015398:Signal Transduction; D012903:Smell / Q000502:physiology
chemical_list: D049452:Green Fluorescent Proteins; D002118:Calcium