title: Graphene microelectrode arrays for neural activity detection.
abstract: We demonstrate a method to fabricate graphene microelectrode arrays (MEAs) using a simple and inexpensive method to solve the problem of opaque electrode positions in traditional MEAs, while keeping good biocompatibility. To study the interface differences between graphene-electrolyte and gold-electrolyte, graphene and gold electrodes with a large area were fabricated. According to the simulation results of electrochemical impedances, the gold-electrolyte interface can be described as a classical double-layer structure, while the graphene-electrolyte interface can be explained by a modified double-layer theory. Furthermore, using graphene MEAs, we detected the neural activities of neurons dissociated from Wistar rats (embryonic day 18). The signal-to-noise ratio of the detected signal was 10.31 ± 1.2, which is comparable to those of MEAs made with other materials. The long-term stability of the MEAs is demonstrated by comparing differences in Bode diagrams taken before and after cell culturing.
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Du
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Ji
authors.initials: J
authors.lastname: Cheng
authors.forename: Shanluo
authors.initials: S
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Cai
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Jin
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000200:Action Potentials; D000818:Animals; D017097:Electric Impedance; D004594:Electrophysiology / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006046:Gold; D006108:Graphite