title: Comparison of amino acid profiles between rats subjected to forced running and voluntary running exercises.
abstract: It has been suspected that in comparison with glucose or fatty acids, the levels of amino acids may readily change with different forms of exercise. In the present study, we measured the concentrations of amino acids, glucose, triglycerides, total protein and total cholesterol in the blood and/or cerebrospinal fluid (CSF) of rats subjected to forced running exercise on a treadmill, and voluntary running exercise using a wheel, with a constant running distance of 440 m. Rats that performed no running and rats subjected to immobilization stress were used as controls. We observed a few significant changes in the levels of plasma glucose, triglycerides, total protein and total cholesterol in all groups. Whereas, plasma amino acid levels were significantly changed by exercise and stress, especially during the light period. The plasma levels of many amino acids were specifically increased by forced running; some were decreased by immobilization stress. Few amino acids showed similar changes in their levels as a result of voluntary running. In addition, there was a significant difference in the degree of amino acid imbalance between blood and CSF. These results provide the first information on changes in levels of amino acids in plasma and CSF resulting from forced and voluntary exercises.
authors.affiliation: Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Rieko
authors.initials: R
authors.lastname: Okame
authors.forename: Keiko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kato
authors.forename: Makoto
authors.initials: M
authors.lastname: Bannai
authors.forename: Noboru
authors.initials: N
authors.lastname: Murakami
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000134:cerebrospinal fluid; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002556:Cerebrospinal Fluid Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood / Q000134:cerebrospinal fluid; D002853:Chromatography, Liquid; D005947:Glucose / Q000134:cerebrospinal fluid; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012420:Running / Q000502:physiology*; D013315:Stress, Psychological / Q000503:physiopathology*; D053719:Tandem Mass Spectrometry; D014280:Triglycerides / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D014280:Triglycerides; D002784:Cholesterol; D005947:Glucose