title: Formulation of highly functionalizable DNA nanoparticles based on 1,2-dithiolane derivatives.
abstract: We describe the formulation of synthetic virus models based on ionic compounds bearing the polymerizable 1,2-dithiolane moiety. First, cationic amphiphiles containing the polymeric inducer were prepared and used to efficiently condense a DNA plasmid (pDNA) into a highly monodisperse population of small polymeric cationic DNA nanoparticles (NPs; Dh ∼100 nm). These nonspecific cationic particles were then functionalized with anionic PEGylated conjugates, also based on the 1,2-dithiolane motifs, in order to produce stable and fully dispersible stealth DNA nanoparticles. Our results show that both ionic interactions and polymerization based on the 1,2-dithiolane pattern occur and that they produce highly functionalizable nonviral DNA NPs.
authors.affiliation: Institut de Chimie de Nice, équipe Molécules Bioactives (Vectorisation & Diagnostic), CNRS UMR 7272, Université de Nice-Sophia Antipolis, Faculté des Sciences, 06108 Nice Cedex 2 (France).
authors.forename: Coralie
authors.initials: C
authors.lastname: Charrat
authors.forename: Anaïs
authors.initials: A
authors.lastname: Biscotti
authors.forename: Guilhem
authors.initials: G
authors.lastname: Godeau
authors.forename: Jacques
authors.initials: J
authors.lastname: Greiner
authors.forename: Pierre
authors.initials: P
authors.lastname: Vierling
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Guigonis
authors.forename: Christophe
authors.initials: C
authors.lastname: Di Giorgio
mesh_terms: D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids; D011092:Polyethylene Glycols; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C448825:1,2-dithiolane; D002412:Cations; D011108:Polymers; D011092:Polyethylene Glycols; D008063:Thioctic Acid; D004247:DNA
keywords: DNA; functionalization; lipoic acid derivatives; nanoparticles; polyanionic PEGylated conjugate; polymerizable detergents