title: [The influence of fibroblast growth factor (FGF2) on cardiomyocytes differentiation of mesenchymal stem cells of bone marrow ex vivo].
abstract: The influence of FGF2 on the efficiency of cardiomyocytes differentiation of mesenchymal stem cells (MSC) of bone marrow induced by 5-azacetidine (5-aza) was studied. The effect of FGF2 developing by the 14th day after the combined action of a differentiating agent and growth factor was manifested in an increase in Mef2A, Mef2D and gene transcription and a rise of ionized Ca2+ concentration in cytoplasm keeping cell viability and proliferation activity. In the presence of FGF2 this approach provided cardiomyogenesis and the increase in the formation of early precursors of cardiomyocytes.
authors.forename: E S
authors.initials: ES
authors.lastname: Lobanok
authors.forename: Z B
authors.initials: ZB
authors.lastname: Kvacheva
authors.forename: S V
authors.initials: SV
authors.lastname: Pinchuk
authors.forename: M V
authors.initials: MV
authors.lastname: Volk
authors.forename: L M
authors.initials: LM
authors.lastname: Mezhevkina
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
authors.forename: I D
authors.initials: ID
authors.lastname: Volotovski
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D064326:MEF2 Transcription Factors / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D064326:MEF2 Transcription Factors; C578037:MEF2A protein, rat; C578045:MEF2D protein, rat; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D001374:Azacitidine; D002118:Calcium