title: Antioxidative effect of ginseng stem-leaf saponins on oxidative stress induced by cyclophosphamide in chickens.
abstract: Previous investigation demonstrated that oral administration of ginseng stem-leaf saponins in chickens could enhance the immune response. The present study was designed to evaluate the effects of ginseng stem-leaf saponins on oxidative stress induced by cyclophosphamide in chickens. One hundred and twenty chickens were randomly divided into 5 groups. Groups 1 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress while group 5 was injected with saline solution and served as control. Following administration of cyclophosphamide, groups 1 to 3 were orally administered ginseng stem-leaf saponins at 2.5, 5, and 10 mg/kg BW in drinking water for 7 d, respectively. After that, the spleen, thymus, bursa, and serum were collected to measure the indices of the organs and oxidative parameters. The results showed that ginseng stem-leaf saponins significantly inhibited cyclophosphamide-induced oxidative stress by increasing the organ indices, total antioxidant capacity, and the levels of glutathione, ascorbic acid, and α-tocopherol, while elevating the activity of total superoxide dismutase, catalase, and glutathione peroxidase, as well as decreasing the protein carbonyl content and malondialdehyde. Therefore, ginseng stem-leaf saponins could be a promising agent against oxidative stress in the poultry industry.
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: L
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China songhua@zju.edu.cn.
authors.forename: S
authors.initials: S
authors.lastname: Hu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002060:Bursa of Fabricius / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D003520:Cyclophosphamide / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000633:toxicity; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D011201:Poultry Diseases / Q000139:chemically induced; D050050:Protein Carbonylation / Q000187:drug effects; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects
chemical_list: D000975:Antioxidants; D007166:Immunosuppressive Agents; D012503:Saponins; D008315:Malondialdehyde; D003520:Cyclophosphamide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: cyclophosphamide; ginseng; oxidative stress