title: Osteoblast biocompatibility of novel chitosan crosslinker, hexamethylene-1,6-diaminocarboxysulfonate.
abstract: Chitosan is a naturally occurring polysaccharide, which has proven to be an attractive candidate for bone tissue engineering, due to its ability to promote osteoblast mineralization. Electrospinning has become a well-established cell scaffold processing technique, as it produces a high surface area to volume fibrous material that can mimic the three dimensionality of the extracellular matrix of a cell. In this study, we have investigated the osteoblast response to two different chemically crosslinked (hexamethylene-1,6-diaminocarboxysulfonate (HDACS) and genipin) electrospun chitosan scaffolds and their film counterparts in order to determine how material chemistry influences cellular behavior in conjunction with material topology. In addition, material properties of each fiber scaffold such as porosity and tensile strength were considered. MLO-A5 osteoblast cells grown on chitosan-HDACS scaffolds were found to display a more organized cellular network, along with significantly more filopodia extensions, compared to those grown on chitosan-genipin scaffolds. After 2 days of growth on chitosan-HDACS fibers, a higher level of alkaline phosphatase expression in MLO-A5 cells was reported compared to that of either chitosan-genipin fibers or films. These results indicate that not only chemistry, but also surface topology is an important effecter of cellular behavior. Ultimately, chitosan-HDACS fiber scaffolds provided an adequate substrate for osteoblast attachment and proliferation.
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania, 19104.
authors.forename: Laura T
authors.initials: LT
authors.lastname: Beringer
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania, 19104.
authors.forename: Marjorie A
authors.initials: MA
authors.lastname: Kiechel
authors.affiliation: Department of Biology, Temple University, 1900 North 12th Street, Philadelphia, Pennsylvania, 19122.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Komiya
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania, 19104.
authors.forename: Amalie E
authors.initials: AE
authors.lastname: Donius
authors.affiliation: Department of Biology, Temple University, 1900 North 12th Street, Philadelphia, Pennsylvania, 19122.
authors.forename: Raymond
authors.initials: R
authors.lastname: Habas
authors.affiliation: Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, New Hampshire, 03755.
authors.forename: Ulrike G K
authors.initials: UG
authors.lastname: Wegst
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania, 19104.
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Schauer
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003959:Diamines / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D003432:Cross-Linking Reagents; D003959:Diamines; D048271:Chitosan; D000469:Alkaline Phosphatase; C007297:1,6-diaminohexane
keywords: bone tissue engineering; chitosan; crosslinking