title: Evaluation of Immune Restoration Potential of PD-1 Blockers.
abstract: The programmed death 1 (PD-1) receptor and its primary ligand (PD-L1) have crucial roles in tumor-induced immune suppression. PD-1/PD-L1 blockers are designed to restore the immune system and induce potent antitumor effects. In this study we established a direct and reliable method to evaluate the immune restoration potential of human PD-1 blockers. We found anti-human PD-1 antibody could reverse PD-L1 induced suppression of human CD3+ cells proliferation and IL-2 production. This method is suitable for all kinds of PD-1 blockers including antibodies and chemical drugs. This function assay could be easily applied and provide valuable information for drug development.
authors.affiliation: a School of Basic Medical Science , Wuhan University , Wuhan , China.
authors.forename: Weihuang
authors.initials: W
authors.lastname: Liu
authors.forename: Zan
authors.initials: Z
authors.lastname: Tong
mesh_terms: D000328:Adult; D000906:Antibodies / Q000494:pharmacology*; D060890:B7-H1 Antigen / Q000276:immunology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D061026:Programmed Cell Death 1 Receptor / Q000037:antagonists & inhibitors* / Q000276:immunology
chemical_list: D000906:Antibodies; D060890:B7-H1 Antigen; C423236:CD274 protein, human; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor
keywords: PD-1 blocker; drug development; flow cytometry; immune restoration; proliferation index