title: Proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident CD8⁺ T cells responding to infection.
abstract: We report that oral infection with Yersinia pseudotuberculosis results in the development of two distinct populations of pathogen-specific CD8(+) tissue-resident memory T cells (TRM cells) in the lamina propria. CD103(-) T cells did not require transforming growth factor-β (TGF-β) signaling but were true resident memory cells. Unlike CD103(+)CD8(+) T cells, which were TGF-β dependent and were scattered in the tissue, CD103(-)CD8(+) T cells clustered with CD4(+) T cells and CX3CR1(+) macrophages and/or dendritic cells around areas of bacterial infection. CXCR3-dependent recruitment of cells to inflamed areas was critical for development of the CD103(-) population and pathogen clearance. Our studies have identified the 'preferential' development of CD103(-) TRM cells in inflammatory microenvironments within the lamina propria and suggest that this subset has a critical role in controlling infection.
authors.affiliation: Department of Immunology and Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA.
authors.forename: Tessa
authors.initials: T
authors.lastname: Bergsbaken
authors.affiliation: Department of Immunology and Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bevan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D002465:Cell Movement; D060833:Cellular Microenvironment; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005786:Gene Expression Regulation; D007156:Immunologic Memory; D016130:Immunophenotyping; D039001:Integrin alpha Chains / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054367:Receptors, CXCR3 / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000276:immunology; D015012:Yersinia pseudotuberculosis Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
chemical_list: D015703:Antigens, CD; C515188:Cxcr3 protein, mouse; D039001:Integrin alpha Chains; D054367:Receptors, CXCR3; D016212:Transforming Growth Factor beta; C091367:alpha E integrins