title: Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates.
abstract: Antimicrobial properties of extracts of underground parts of three Laserpitium L. (Apiaceae) species, namely Laserpitium latifolium L., Laserpitium zernyi Hayek and Laserpitium ochridanum Micevski, were investigated. The investigated species are widely used as functional foods, as spices and for preparations in traditional medicine for treating complaints connected with infection and inflammation. Furthermore, antimicrobial and antibiofilm effects of laserpitine, the most abundant compound in the chloroform extract of L. latifolium, and guaianolide sesquiterpene lactones, such as, isomontanolide, montanolide and tarolide, principal components of the extracts of L. zernyi and L. ochridanum were assessed. The antimicrobial activity was tested using the microdilution method against five pathogenic bacteria and five fungi, as well as in the microplate biofilm assay on two Candida clinical isolates (C. albicans and C. krusei). Among the extracts, L. latifolium showed the most prominent activity. Isolated metabolites exerted higher effects against fungal than against bacterial strains, isomontanolide being the most active. Interestingly, all constituents showed higher potential on inhibition of biofilm formation than fluconazole, a reference compound. Tested metabolites may be good novel agents with high antifungal and antibacterial potential that might find practical applications in food industry as food preservatives in order to retard the growth of food spoiling microbes, but only after detailed safety assessments.
authors.affiliation: University of Belgrade - Faculty of Pharmacy, Department of Pharmacognosy, V. Stepe 450, 11221 Belgrade, Serbia.
authors.forename: Višnja
authors.initials: V
authors.lastname: Popović
authors.forename: Dejan
authors.initials: D
authors.lastname: Stojković
authors.forename: Miloš
authors.initials: M
authors.lastname: Nikolić
authors.forename: Arne
authors.initials: A
authors.lastname: Heyerick
authors.forename: Silvana
authors.initials: S
authors.lastname: Petrović
authors.forename: Marina
authors.initials: M
authors.lastname: Soković
authors.forename: Marjan
authors.initials: M
authors.lastname: Niketić
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D019661:Apiaceae / Q000737:chemistry*; D001232:Aspergillus fumigatus / Q000187:drug effects; D001234:Aspergillus niger / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D007783:Lactones / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010407:Penicillium / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D025341:Principal Component Analysis; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D012717:Sesquiterpenes / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: C000605324:6,10-diangeloyloxy-4-hydroxydihydrodaucane-9-one; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; C000605325:isomontanolide; C000605326:montanolide; C000605327:tarolide