title: Viable pigs with a conditionally-activated oncogenic KRAS mutation.
abstract: Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRAS (G12D) mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRAS (G12D) animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.
authors.affiliation: Chair of Livestock Biotechnology, Technische Universität München, Liesel-Beckmann-Straße 1, 85354, Freising, Germany.
authors.forename: Shun
authors.initials: S
authors.lastname: Li
authors.forename: Marlene
authors.initials: M
authors.lastname: Edlinger
authors.forename: Anja
authors.initials: A
authors.lastname: Saalfrank
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Flisikowski
authors.forename: Alexander
authors.initials: A
authors.lastname: Tschukes
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kurome
authors.forename: Valeri
authors.initials: V
authors.lastname: Zakhartchenko
authors.forename: Barbara
authors.initials: B
authors.lastname: Kessler
authors.forename: Dieter
authors.initials: D
authors.lastname: Saur
authors.forename: Alexander
authors.initials: A
authors.lastname: Kind
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.forename: Angelika
authors.initials: A
authors.lastname: Schnieke
authors.forename: Tatiana
authors.initials: T
authors.lastname: Flisikowska
mesh_terms: D000818:Animals; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009154:Mutation; D053652:Nuclear Transfer Techniques; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics*; D018631:ras Proteins / Q000235:genetics*
chemical_list: D011518:Proto-Oncogene Proteins; C045073:Cre recombinase; D019426:Integrases; D018631:ras Proteins