title: Designing primers for SNaPshot technique.
abstract: The SNaPshot technique, also known as minisequencing, is a primer extension-based method developed for the analysis of Single Nucleotide Polymorphisms (SNPs). Using this technique, it is possible to analyze more than 50 SNPs distributed throughout the genome in a single multiplex reaction, making it an advantage when compared with traditional sequencing reaction. In this chapter, you will find a step-by-step guide to design a multiplex primer assay for SNaPshot reaction.
authors.affiliation: Department of Pharmacy and Nutrition, Federal University of Espirito Santo, Room 18, Alto Universitario s/n, CCA, Alegre, ES, 29500-000, Brazil, greiciane.paneto@ufes.br.
authors.forename: Greiciane Gaburro
authors.initials: GG
authors.lastname: Paneto
authors.forename: Francisco
authors.initials: F
authors.lastname: de Paula Careta
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers