title: Biocompatibility of a new epoxy resin-based root canal sealer in subcutaneous tissue of rat.
abstract: OBJECTIVE
  The objective was to evaluate the subcutaneous biocompatibility of two root canal sealers.
  
  
  METHODS
  Thirty female rats were distributed into two groups of 15 animals. Each received subcutaneous dorsal implants: Silicone tubes filled with AH Plus or Obtuseal. After the 11(th), 14(th), and 45(th) days, tissues were collected for biopsy and fixed and processed for histologic evaluation. Observations of the cellular inflammatory components, such as lymphocytes and macrophages, were made. The data were analyzed using Kruskal-Wallis and Connover tests (P < 0.05).
  
  
  RESULTS
  By the 7(th) day, lymphocyte infiltration increased in both groups (P = 0.0104). However, macrophage infiltration increased only in the AH Plus group (P = 0.0011). By the 14(th) and 45(th) days, lymphocyte and macrophage infiltrations were reduced. At the end of the experimental period, no statistically significant differences were found between the AH Plus group and the Obtuseal group (P > 0.05).
  
  
  CONCLUSIONS
  After 45 days, Obtuseal yielded a satisfactory tissue reaction; it was biocompatible when tested in subcutaneous rat tissue. On the basis of the obtained results, it was concluded that Obtuseal root canal sealer caused a satisfactory tissue reaction as AH plus, because it was biocompatible when tested in the subcutaneous tissue of rats.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Inonu University, Malatya, Turkiye.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Simsek
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Inonu University, Malatya, Turkiye.
authors.forename: Levent
authors.initials: L
authors.lastname: Akinci
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Inonu University, Malatya, Turkiye.
authors.forename: Orhan
authors.initials: O
authors.lastname: Gecor
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Inonu University, Malatya, Turkiye.
authors.forename: Hilal
authors.initials: H
authors.lastname: Alan
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Inonu University, Malatya, Turkiye.
authors.forename: Fuat
authors.initials: F
authors.lastname: Ahmetoglu
authors.affiliation: Department of Histology and Embriology, Faculty of Medicine, Inonu University, Malatya, Turkiye.
authors.forename: Elif
authors.initials: E
authors.lastname: Taslidere
keywords: Biocompatibility; endodontics; epoxy resin-based sealers; tissue reaction