title: Peroxisomal Pex3 activates selective autophagy of peroxisomes via interaction with the pexophagy receptor Atg30.
abstract: Pexophagy is a process that selectively degrades peroxisomes by autophagy. The Pichia pastoris pexophagy receptor Atg30 is recruited to peroxisomes under peroxisome proliferation conditions. During pexophagy, Atg30 undergoes phosphorylation, a prerequisite for its interactions with the autophagy scaffold protein Atg11 and the ubiquitin-like protein Atg8. Atg30 is subsequently shuttled to the vacuole along with the targeted peroxisome for degradation. Here, we defined the binding site for Atg30 on the peroxisomal membrane protein Pex3 and uncovered a role for Pex3 in the activation of Atg30 via phosphorylation and in the recruitment of Atg11 to the receptor protein complex. Pex3 is classically a docking protein for other proteins that affect peroxisome biogenesis, division, and segregation. We conclude that Pex3 has a role beyond simple docking of Atg30 and that its interaction with Atg30 regulates pexophagy in the yeast P. pastoris.
authors.affiliation: From the Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0322.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Burnett
authors.affiliation: From the Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0322.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Farré
authors.affiliation: From the Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0322.
authors.forename: Taras Y
authors.initials: TY
authors.lastname: Nazarko
authors.affiliation: From the Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0322 ssubramani@ucsd.edu.
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramani
mesh_terms: D001343:Autophagy; D005656:Fungal Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins
keywords: Autophagy; Membrane Trafficking; Peroxisome; Pexophagy; Protein-Protein Interaction; Receptor Regulation; Signaling