title: Epigallocatechin-3-gallate protects against cisplatin-induced nephrotoxicity by inhibiting endoplasmic reticulum stress-induced apoptosis.
abstract: Cisplatin (CP)-induced nephrotoxicity hampers its application in clinic. Green tea, particularly its predominant polyphenolic constituent epigallocatechin-3-gallate (EGCG), possesses anti-inflammatory, antioxidant, and anti-apoptotic properties. The present study was designed to investigate the protective effects of EGCG against CP-induced nephrotoxicity in mice. Male C57/BL6 mice in different groups received single injection of CP (20 mg/kg) and EGCG (100 mg/kg) in various sets and kidney tissues and blood were collected after killing. Then, samples were used for biochemical and immunohistochemical assay. Our results showed EGCG decreased biochemical factors and immunohistochemical damage induced by CP. Besides, expression of phosphorylated-extracellular signal-regulated kinase (p-ERK), glucose-regulated protein 78 (GRP78), caspase-12, and apoptosis of kidney were decreased by EGCG via inhibition of endoplasmic reticulum (ER) stress-induced apoptosis.
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China Ministry of Education Key Laboratory of Experimental Teratology and Institute of Molecular Medicine and Genetics, Shandong University School of Medicine, Jinan, Shandong 250013, China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Peimei
authors.initials: P
authors.lastname: Zou
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Qiufa
authors.initials: Q
authors.lastname: Hao
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, China huzhaoql@sina.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D000970:Antineoplastic Agents / Q000009:adverse effects; D000975:Antioxidants / Q000737:chemistry; D017209:Apoptosis; D053454:Caspase 12 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002945:Cisplatin / Q000009:adverse effects*; D004195:Disease Models, Animal; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D002392:Catechin; C045651:epigallocatechin gallate; D048049:Extracellular Signal-Regulated MAP Kinases; D053454:Caspase 12; D002945:Cisplatin
keywords: Cisplatin; apoptosis; endoplasmic reticulum stress; epigallocatechin-3-gallate; nephrotoxicity