title: Fast gamma oscillations are generated intrinsically in CA1 without the involvement of fast-spiking basket cells.
abstract: Information processing in neuronal networks relies on the precise synchronization of ensembles of neurons, coordinated by the diverse family of inhibitory interneurons. Cortical interneurons can be usefully parsed by embryonic origin, with the vast majority arising from either the caudal or medial ganglionic eminences (CGE and MGE). Here, we examine the activity of hippocampal interneurons during gamma oscillations in mouse CA1, using an in vitro model where brief epochs of rhythmic activity were evoked by local application of kainate. We found that this CA1 KA-evoked gamma oscillation was faster than that in CA3 and, crucially, did not appear to require the involvement of fast-spiking basket cells. In contrast to CA3, we also found that optogenetic inhibition of pyramidal cells in CA1 did not significantly affect the power of the oscillation, suggesting that excitation may not be essential for gamma genesis in this region. We found that MGE-derived interneurons were generally more active than CGE interneurons during CA1 gamma, although a group of CGE-derived interneurons, putative trilaminar cells, were strongly phase-locked with gamma oscillations and, together with MGE-derived axo-axonic and bistratified cells, provide attractive candidates for being the driver of this locally generated, predominantly interneuron-driven model of gamma oscillations.
authors.affiliation: Program in Developmental Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Michael T
authors.identifier: http://orcid.org/0000-0001-8481-6709
authors.initials: MT
authors.lastname: Craig
authors.affiliation: Program in Developmental Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 mcbainc@mail.nih.gov.
authors.forename: Chris J
authors.identifier: http://orcid.org/0000-0002-5909-0157
authors.initials: CJ
authors.lastname: McBain
mesh_terms: D000200:Action Potentials; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D056654:CA3 Region, Hippocampal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D065818:Gamma Rhythm; D007395:Interneurons / Q000502:physiology*; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D009928:Organ Specificity; D017966:Pyramidal Cells / Q000502:physiology*
chemical_list: D007608:Kainic Acid
keywords: axo-axonic; basket; gamma; interneurons; oscillation; trilaminar