title: The effect of superoxide dismutase mimetic and catalase on the quality of postthawed goat semen.
abstract: Manganese(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin chloride (MnTE) is a cell-permeable superoxide dismutase mimetic agent which can convert superoxide to hydrogen peroxide (H2O2). Supplementation of MnTE to a commercial semen extender can protect sperm from superoxide but not H2O2. Therefore, we proposed that addition of catalase (0.0, 200, or 400 IU/mL) in combination with MnTE (0.1 μM) may further improve the cryopreservation efficiency of goat semen in commercially optimized freezing media such as Andromed. Therefore, ejaculates were obtained from three adult bucks twice a week during the breeding season and diluted with Andromed supplemented with or without MnTE and catalase and were frozen in liquid nitrogen. Sperm parameters and reactive oxygen species contents were evaluated 2 hours after dilution (before freezing) and after freezing/thawing. The results revealed that all the treatments significantly (P ≤ 0.05) improved sperm motility, viability, and membrane integrity after freezing and reduced reactive oxygen species content compared with the control group, but maximum improvement was obtained in MnTE + 400 IU/mL catalase. In addition, supplementation with these antioxidants significantly (P ≤ 0.05) increases the cleavage rate after IVF. In conclusion, the results of present study suggest that addition of antioxidant MnTE or catalase to commercial optimized media, such as Andromed, improves total motility, membrane integrity, and viability of goat semen samples after thawing. But the degree of improvement for these parameters significantly (P ≤ 0.05) higher when MnTE and catalase were simultaneously added to the cryopreservation media.
authors.affiliation: Department of Biochemistry, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shafiei
authors.affiliation: Department of Biochemistry, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Forouzanfar
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Sayyed Morteza
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address: mh.nasresfahani@royaninstitute.org.
authors.forename: Mohammad Hossein Nasr
authors.initials: MH
authors.lastname: Esfahani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006041:Goats; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility; D013482:Superoxide Dismutase; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D008665:Metalloporphyrins; D017382:Reactive Oxygen Species; C431023:manganese (III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin; D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant; MnTE; Reactive oxygen species; Semen cryopreservation; Sperm motility