title: Leishmania donovani skews the CD56(+) Natural Killer T cell response during human visceral leishmaniasis.
abstract: The objective of this study was to understand the categorical function of CD4(+)CD56(+) and CD8(+)CD56(+) NKT cells in human visceral leishmaniasis. These cell populations were significantly deregulated in human peripheral blood during VL. The in vitro experiments showed that CD4(+)NKT cells, but not CD8(+)NKT cells, migrated towards the Leishmania donovani infection site. Additionally, CD4(+)NKT cells from VL subjects primarily expressed CD25(+)Foxp3 and IL-10 compared with healthy subjects. However, CD8(+)NKT cells expressed primarily IFN-γ and killer cell immunoglobulin receptor compared with healthy subjects. Because the ratio of CD4(+) and CD8(+)NKT cells was 1:10, adoptive transfer of CD3(+)CD56(+) NKT cells effectively reduced the L. donovani burden in infected macrophages. This study concludes that although CD4(+)NKT cells are pathogenic and accumulate at the infection site, CD8(+)NKT cells may be protective in contact with target cells.
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Sarita
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Fauzia
authors.initials: F
authors.lastname: Jamal
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Pushkar
authors.initials: P
authors.lastname: Shivam
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: Sanjiva
authors.initials: S
authors.lastname: Bimal
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: V N R
authors.initials: VN
authors.lastname: Das
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: K
authors.initials: K
authors.lastname: Pandey
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: S
authors.initials: S
authors.lastname: Narayan
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India.
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Agamkuan, Patna 800007, India. Electronic address: sksrmri@yahoo.com.
authors.forename: Shubhankar K
authors.initials: SK
authors.lastname: Singh
mesh_terms: D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019002:CD56 Antigen / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D055611:Natural Killer T-Cells / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Leishmania donovani; Natural Killer T cell; Visceral leishmaniasis