title: Characterization of LpGPAT gene in Lilium pensylvanicum and response to cold stress.
abstract: LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
authors.affiliation: Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biological Science and Technology, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China ; Key Laboratory of Protected Horticulture, College of Horticulture, Ministry of Education, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China.
authors.forename: Shao-kun
authors.initials: SK
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biological Science and Technology, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China.
authors.forename: Ni-na
authors.initials: NN
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biological Science and Technology, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China ; Key Laboratory of Protected Horticulture, College of Horticulture, Ministry of Education, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China.
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biological Science and Technology, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Irfan
authors.affiliation: Liaoning Academy of Agricultural Sciences, Shenyang 110161, China.
authors.forename: Xing-hua
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Protected Horticulture, College of Horticulture, Ministry of Education, Shenyang Agriculture University, 120 Dongling Road, Shenyang, Liaoning 110866, China.
authors.forename: Tian-lai
authors.initials: TL
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D058639:Cold-Shock Response / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000737:chemistry* / Q000378:metabolism*; D027762:Lilium / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D005992:Glycerol-3-Phosphate O-Acyltransferase