title: Aging-induced changes in sex-steroidogenic enzymes and sex-steroid receptors in the cortex, hypothalamus and cerebellum.
abstract: We investigated age-induced changes in mRNA expression profiles of sex-steroidogenic enzymes and sex-steroid receptors in 3-, 12-, and 24-month-old male rat brain subregions [cerebral cortex (CC), hypothalamus (Hy) and cerebellum (CL)]. In many cases, the expression levels of mRNA decreased with age for androgen synthesis enzyme systems, including Cyp17a1, Hsd17b and Srd5a in the CC and CL, but not in the Hy. Estradiol synthase Cyp19a1 did not show age-induced decline in the Hy, and nearly no expression of Cyp19a1 was observed in the CC and CL over 3-24 m. Androgen receptor Ar increased in the Hy but decreased in the CC with age. Estrogen receptor Esr1 increased in the CC and Hy, and did not change in the CL with age. Esr2 did not change in the CC and Hy, but decreased in the CL with age. As a comparison, age-induced changes of brain-derived neurotrophic factor mRNA were also investigated.
authors.affiliation: Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, Univ. Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Munetomo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hojo
authors.forename: Shimpei
authors.initials: S
authors.lastname: Higo
authors.forename: Asami
authors.initials: A
authors.lastname: Kato
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Takuji
authors.initials: T
authors.lastname: Shirasawa
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
authors.forename: Anna
authors.initials: A
authors.lastname: Barron
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimoto
authors.forename: Suguru
authors.initials: S
authors.lastname: Kawato
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012739:Gonadal Steroid Hormones; D008565:Membrane Proteins; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D001141:Aromatase; C582555:CYP17A1 protein, rat; D013254:Steroid 17-alpha-Hydroxylase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C548474:Srd5a2 protein, rat