title: Susceptibility of Aedes albopictus and Aedes aegypti to three imported Chikungunya virus strains, including the E1/226V variant in Taiwan.
abstract: OBJECTIVE
  An E1/226V variant Chikungunya virus (CHIKV) efficiently transmitted by Aedes albopictus to humans poses a significant threat to public health for those areas with the presence of Aedes albopictus, including Taiwan.
  
  
  METHODS
  We infected three imported CHIKV isolates including the E1/226V variant with Ae. albopictus and Aedes aegypti in the laboratory to understand the disease risk. Viral RNA was measured by real time reverse transcription polymerase chain reaction.
  
  
  RESULTS
  The viral susceptibility varied by virus strain and mosquito species and strain. The Asian virus strain started to replicate at 5-6 days post infection (dpi) with the maximum virus yield, ranging from 10(3.63) to 10(3.87) at 5-10 dpi in both species. The variant CHIKV Central/East/South African (CESA) virus genotype replicated earlier at 1 dpi with the maximum virus yield ranging from 10(5.63) to 10(6.52) at 3-6 dpi in Ae. albopictus females while the nonvariant virus strain replicated at 1-2 dpi with the maximum virus yield ranging from 10(5.51) to 10(6.27) at 6-12 dpi. In Ae. aegypti, these viruses replicated at 1-2 dpi, with maximum yields at 4-5 dpi (range from 10(5.38) to 10(5.62)).
  
  
  CONCLUSIONS
  We concluded that the risk of CHIKV in Taiwan is high in all distribution areas of Ae. aegypti and Ae. albopictus for the CESA genotype and that the E1/226V variant virus strain presents an even higher risk.
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Tien-Huang
authors.initials: TH
authors.lastname: Chen
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Shu-Wan
authors.initials: SW
authors.lastname: Jian
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Cheo
authors.initials: C
authors.lastname: Lin
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Pei-Feng
authors.initials: PF
authors.lastname: Wang
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Chien-Ling
authors.initials: CL
authors.lastname: Su
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC. Electronic address: hjteng@cdc.gov.tw.
authors.forename: Hwa-Jen
authors.initials: HJ
authors.lastname: Teng
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Pei-Yun
authors.initials: PY
authors.lastname: Shu
authors.affiliation: Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Number 161, Kunyang Street, Taipei 11561, Taiwan, ROC.
authors.forename: Ho-Sheng
authors.initials: HS
authors.lastname: Wu
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000235:genetics*; D005260:Female; D012367:RNA, Viral / Q000302:isolation & purification; D013624:Taiwan
chemical_list: D012367:RNA, Viral
keywords: Aedes aegypti; Aedes albopictus; Chikungunya virus; Taiwan; mosquito infection