title: PDGF signalling in the dermis and in dermal condensates is dispensable for hair follicle induction and formation.
abstract: Embryonic hair follicle (HF) induction and formation is dependent on signalling crosstalk between the dermis and specialized dermal condensates on the mesenchymal side and epidermal cells and incipient placodes on the epithelial side, but the precise nature and succession of signals remain unclear. Platelet-derived growth factor (PDGF) signalling is involved in the development of several organs and the maintenance of adult tissues, including HF regeneration in the hair cycle. As both PDGF receptors, PDGFRα and PDGFRβ, are expressed in embryonic dermis and dermal condensates, we explored in this study the role of PDGF signalling in HF induction and formation in the developing skin mesenchyme. We conditionally ablated both PDGF receptors with Tbx18(Cre) in early dermal condensates before follicle formation, and with Prx1-Cre broadly in the ventral dermis prior to HF induction. In both PDGFR double mutants, HF induction and formation ensued normally, and the pattern of HF formation and HF numbers were unaffected. These data demonstrate that mesenchymal PDGF signalling, either in the specialized niche or broadly in the dermis, is dispensable for HF induction and formation.
authors.affiliation: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Amélie
authors.initials: A
authors.lastname: Rezza
authors.affiliation: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Sennett
authors.affiliation: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Manon
authors.initials: M
authors.lastname: Tanguy
authors.affiliation: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Clavel
authors.affiliation: Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Rendl
mesh_terms: D000818:Animals; D020405:Dermis / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D055786:Gene Knockout Techniques; D018859:Hair Follicle / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D008648:Mesoderm / Q000166:cytology / Q000196:embryology / Q000502:physiology; D051379:Mice; D008817:Mice, Mutant Strains; D023421:Models, Animal; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000502:physiology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000502:physiology; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D010982:Platelet-Derived Growth Factor; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta
keywords: PDGF signalling; dermal papilla cells; hair follicle morphogenesis; hair follicle stem cells; stem cell niche