title: Comparison of γ-irradiation with other pretreatments followed with simultaneous saccharification and fermentation on bioconversion of microcrystalline cellulose for bioethanol production.
abstract: The effect of γ-irradiation pretreatment was compared with other pretreatment methods including ionic liquids (ILs), 1% HCl, 1% H2SO4, acidic aqueous Ils (AA-ILs), on the bioconversion efficiency of microcrystalline cellulose (MCC) for bioethanol production. The efficiency of MCC pretreatment followed with simultaneous saccharification and fermentation (SSF) was firstly evaluated according to the variations of the irradiation-derived compounds and structure of MCC, as well as yeast growth curve and bioethanol yield. Results showed that the appropriate irradiation dose (891 kGy used in our work) could eliminate the negative effect of toxic irradiation-derived compounds on SSF for ethanol bioconversion with the yield value of 67%. Analyses of SEM, FT-IR, reducing sugar and bioethanol yield showed that the efficiency of pretreatment on MCC was ILs ≈ irradiation pretreatment > AA-ILs pretreatment > 1% HCl pretreatment > 1% H2SO4 pretreatment.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: liuyun@mail.buct.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Hunan Institute of Nuclear Agricultural Science and Space Breeding, Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, Changsha 410125, China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Wang
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Su
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005720:Gamma Rays; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D056804:Biofuels; D000431:Ethanol; D002482:Cellulose; C109691:microcrystalline cellulose
keywords: Bioethanol; Microcrystalline cellulose (MCC); Simultaneous saccharification and fermentation (SSF); γ-Irradiation pretreatment