title: Pentoxifylline and prostaglandin E1 action on ischemia and reperfusion of small intestine tissue in rats. An immunohistochemical study.
abstract: OBJECTIVE
  To investigate the action of pentoxifylline (PTX) and prostaglandin E1 (PGE1) on ischemia and reperfusion of small intestine tissue in rats, using immunohistochemical analysis.
  
  
  METHODS
  Thirty-five Wistar rats were distributed as follows: group A (n=10): subjected to intestinal ischemia and reperfusion for 60 min, with no drugs; group B (n=10): PTX given during tissue ischemia and reperfusion; group C (n=10): PGE1 given during tissue ischemia and reperfusion; group D (n=5): sham. A segment of the small intestine was excised from each euthanized animal and subjected to immunohistochemical examination.
  
  
  RESULTS
  Mean number of cells expressing anti-FAS ligand in the crypts was highest in Group A (78.9 ± 17.3), followed by groups B (16.7 ± 2.8), C (11.3 ± 1.8), and D (2.5 ± 0.9), with very significant differences between groups (p<0.0001).
  
  
  CONCLUSIONS
  The use of pentoxifylline or prostaglandin E1 proved beneficial during tissue reperfusion. The immunohistochemical results demonstrated a decrease in apoptotic cells, while protecting other intestinal epithelium cells against death after reperfusion, allowing these cells to renew the epithelial tissue.
authors.affiliation: Department of Surgery, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: José Lacerda
authors.initials: JL
authors.lastname: Brasileiro
authors.affiliation: Postgraduate Program in Health and Development, UFMS, Campo Grande, MS, Brazil.
authors.forename: Rondon Tosta
authors.initials: RT
authors.lastname: Ramalho
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Luis Carlos
authors.initials: LC
authors.lastname: Takita
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Guido
authors.initials: G
authors.lastname: Marks
authors.affiliation: Postgraduate Program in Health and Development, UFMS, Campo Grande, MS, Brazil.
authors.forename: Peterson Vieira de
authors.initials: PV
authors.lastname: Assis
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology; D007421:Intestine, Small / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D010431:Pentoxifylline / Q000494:pharmacology*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D014665:Vasodilator Agents; D000527:Alprostadil; D010431:Pentoxifylline