title: Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.
abstract: Dilute acid-pretreated sorghum bagasse, which was predominantly composed of glucan (59%) and xylose (7.2%), was used as a lignocellulosic feedstock for d-phenyllactic acid (PhLA) production by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. During fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, the PhLA yield was reduced by 35% compared to filter paper hydrolysate, and metabolomics analysis revealed that NAD(P)H regeneration and intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate for PhLA biosynthesis markedly reduced. Compared to separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components, including p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Kawaguchi
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Teramura
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Uematsu
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Hirano
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sazuka
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kitano
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Yota
authors.initials: Y
authors.lastname: Tsuge
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Prihardi
authors.initials: P
authors.lastname: Kahar
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Niimi-Nakamura
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Oinuma
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Takaya
authors.affiliation: Education and Research Center of Alpine Field Science, Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Kasuga
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Turumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007773:Lactates / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D055442:Metabolome; D011422:Propionates; D045868:Sorghum / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D007773:Lactates; D011422:Propionates; C036909:lignocellulose; C017648:3-phenyllactic acid; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; C495469:p-coumaric acid; D005947:Glucose
keywords: Fermentation inhibitor; Lignocellulosic biomass; Phenyllactic acid; Simultaneous saccharification and fermentation; Sorghum