title: Isolation and molecular characterization of 1-aminocyclopropane-1-carboxylic acid synthase genes in Hevea brasiliensis.
abstract: Ethylene is an important factor that stimulates Hevea brasiliensis to produce natural rubber. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a rate-limiting enzyme in ethylene biosynthesis. However, knowledge of the ACS gene family of H. brasiliensis is limited. In this study, nine ACS-like genes were identified in H. brasiliensis. Sequence and phylogenetic analysis results confirmed that seven isozymes (HbACS1-7) of these nine ACS-like genes were similar to ACS isozymes with ACS activity in other plants. Expression analysis results showed that seven ACS genes were differentially expressed in roots, barks, flowers, and leaves of H. brasiliensis. However, no or low ACS gene expression was detected in the latex of H. brasiliensis. Moreover, seven genes were differentially up-regulated by ethylene treatment. These results provided relevant information to help determine the functions of the ACS gene in H. brasiliensis, particularly the functions in regulating ethylene stimulation of latex production.
authors.affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. hjzhu1010@163.com.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Zhu
authors.affiliation: Hainan Academy of Agricultural Sciences, Haikou 571100, China. xujing6732807@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. changwenjun@itbb.org.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Chang
authors.affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. zhangzhili@itbb.org.cn.|Hainan Academy of Agricultural Sciences, Haikou 571100, China. zhangzhili@itbb.org.cn.
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005030:Ethylenes / Q000494:pharmacology; D017343:Genes, Plant; D028482:Hevea / Q000201:enzymology / Q000235:genetics*; D008190:Lyases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D005030:Ethylenes; D011994:Recombinant Proteins; C036216:ethylene; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase