title: Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivum L.).
abstract: Choice of an appropriate promoter is critical to express target genes in intended tissues and developmental stages. However, promoters capable of directing gene expression in specific tissues and stages are not well characterized in monocot species. To identify such a promoter in wheat, this study isolated a partial sequence of the wheat small subunit of RuBisCO (TarbcS) promoter. In silico analysis revealed the presence of elements that are characteristic to rbcS promoters of other, mainly dicot, species. Transient expression of the TarbcS:GUS in immature wheat embryos and tobacco leaves but not in the wheat roots indicate the functionality of the TarbcS promoter fragment in directing the expression of target genes in green plant tissues.
authors.affiliation: a Department of Plant Science ; University of Manitoba ; Winnipeg , Canada.
authors.forename: Shalini
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Claudio
authors.initials: C
authors.lastname: Stasolla
authors.forename: Anita
authors.initials: A
authors.lastname: Brûlé-Babel
authors.forename: Belay T
authors.initials: BT
authors.lastname: Ayele
mesh_terms: D001483:Base Sequence; D017930:Genes, Reporter; D005966:Glucuronidase / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D012639:Seeds / Q000378:metabolism; D014908:Triticum / Q000196:embryology / Q000201:enzymology* / Q000235:genetics*
chemical_list: D021122:Protein Subunits; D005966:Glucuronidase; D012273:Ribulose-Bisphosphate Carboxylase
keywords: promoter; rbcS; reporter gene; tissue specificity; transient expression; wheat