title: Impairment of neutrophil migration to remote inflammatory site during lung histoplasmosis.
abstract: Histoplasma capsulatum (Hc) induces a pulmonary disease in which leukotrienes promote activation and recruitment of effectors cells. It is also well-recognized that leukotriene B4 (LTB4) and platelet-activating factor (PAF) induce leukocyte recruitment to inflammatory sites. We investigated the impact of pulmonary Hc infection on PMN migration to a remote inflammatory site. Our results show that pulmonary Hc infection impairs LTB4- or PAF-stimulated PMN recruitment to air pouch. Yet, remote inflammation did not modify PMN numbers in the bronchoalveolar lavage fluid (BALF) of Hc-infected mice. Interestingly, the concomitant administration of PAF and LTB4 receptor antagonists inhibited PMN recruitment to both BALF and the remote site, demonstrating cooperation between both mediators. Along that line, our results show that PAF-elicited PMN chemotaxis was abrogated in 5-lipoxygenase-deficient animals. These results suggest caution in the indiscriminate use of anti-inflammatory drugs during infectious diseases.
authors.affiliation: School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
authors.forename: Alexandra I
authors.initials: AI
authors.lastname: Medeiros
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Secatto
authors.affiliation: Faculty of Pharmacy, Université de Montréal, Montréal, QC, Canada H3T 1J4.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bélanger
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Sorgi
authors.affiliation: Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHUQ (CHUL), Sainte-Foy, QC, Canada G1V 4G2.
authors.forename: Pierre
authors.initials: P
authors.lastname: Borgeat
authors.affiliation: Faculty of Pharmacy, Université de Montréal, Montréal, QC, Canada H3T 1J4.
authors.forename: Sylvie
authors.identifier: 0000-0002-9392-1989
authors.initials: S
authors.lastname: Marleau
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Lúcia H
authors.initials: LH
authors.lastname: Faccioli
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D002465:Cell Movement / Q000276:immunology; D006660:Histoplasmosis / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018375:Neutrophil Activation / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents