title: Detection of highly ciprofloxacin resistance acinetobacter baumannii isolated from patients with burn wound infections in presence and absence of efflux pump inhibitor.
abstract: The emergence of fluoroquinolone resistance among A. baumannii isolates is now of particular concern. Phenotypic and genotypic characteristics of resistance to ciprofloxacin among 50 Acinetobacter baumannii isolated from burn wound infections of Tehran were evaluated by E-test and broth microdilution in presence and absence of efflux pump inhibitor phenylalanine- arginine β-naphthylamide (PAβN) and PCR-sequencing methods. All isolates were then typed by REP-PCR fingerprinting to find the clonal relationship between resistant isolates. Our results indicated that resistance to ciprofloxacin among A. baumannii isolated from burn infections in Tehran are high with resistance rate of 100% and ciprofloxacin resistant isolates have a mutation of Serine 83 →Leucine in the quinolone resistance determining region (QRDR) of DNA gyrase subunit A (GyrA). 38% of the isolates showed MIC ranges of 64 to ≥512μg/ml and were considered as highly resistant. We could not detect Par C mutations and plasmid-mediated quinolone resistance A (qnrA) among ciprofloxacin resistant isolates. When we used the efflux pump inhibitor PAbN, MIC of ciprofloxacin was reduced two-to four folds. REP-type A (25/50; 50%), B (20/50; 30%) and C (10/50; 20%) were the most common REP-types among A. baumannii isolates. It seems that mutation in GyrA is the main mechanism of resistant to ciprofloxacin among A. baumannii isolates from burn infections and presence of efflux pumps is just secondary target for ciprofloxacin resistant among A. baumannii in Iran. Regarding with limitation of REP-types detected in this study, we found good correlation between resistance to ciprofloxacin and REP-types A-C.
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Mohammad-Hossein
authors.initials: MH
authors.lastname: Maleki
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran ; Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Farid Azizi
authors.initials: FA
authors.lastname: Jalilian
authors.affiliation: Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Hatef
authors.initials: H
authors.lastname: Khayat
authors.affiliation: Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Department of Microbiology, School of Veterinary Medicine, University of Ilam, Ilam, Iran.
authors.forename: Fazel
authors.initials: F
authors.lastname: Pourahmad
authors.affiliation: Department of Epidemiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Khairollah
authors.initials: K
authors.lastname: Asadollahi
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran ; Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Pakzad
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran ; Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Nourkhoda
authors.initials: N
authors.lastname: Sadeghifard
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran ; Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Setareh
authors.initials: S
authors.lastname: Soroush
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
authors.affiliation: Clinical Center of Microbiology Research, Ilam University of Medical Sciences, Ilam, Iran ; Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
keywords: A. baumannii; ciprofloxacin resistance; gyr A QRDRS