title: Synthesis, structural characterization and DNA interaction of zinc complex from 2,6-diacetylpyridine dihydrazone and {4-[(2E)-2-(hydroxyimino)acetyl]phenoxy} acetic acid.
abstract: A new water soluble zinc complex has been prepared and structurally characterized. The Zn(II) complex was synthesized by the reaction of 2,6-diacetylpyridine dihydrazone (dph) with {4-[(2E)-2-(hydroxyimino)acetyl]phenoxy} acetic acid (H₂L) in the presence of zinc(II) acetate. Single crystal X-ray diffraction study revealed that the zinc ion is situated in distorted trigonal-bipyramidal environment where the equatorial position is occupied by the nitrogen atom of pyridine ring and the oxygen atoms of acetate groups of two oxime ligands (H₂L) whereas the axial positions of the zinc complex are occupied by the imine nitrogen atoms of dph ligand. Characterization of the complex with FTIR, (1)H and (13)C NMR, UV-vis and elemental analysis also confirmed the proposed structure. Interaction of the Zn(II) complex with calf-thymus DNA (CT-DNA) was investigated through UV-vis spectroscopy and viscosity measurements. The results suggest that the complex preferably bind to DNA through the groove binding mode. The zinc complex cleaves plasmid pBR 322 DNA in the presence and absence of an oxidative agent (H₂O₂), possibly through a hydrolytic pathway which is also supported by DNA cleave experiments in the presence of different radical scavengers. The nuclease activity of the zinc complex significantly depends on concentration of the complex and incubation time both in the presence and absence of H₂O₂. DNA cleave activity is inhibited in the presence of methyl green indicating that the zinc complex seems to bind the major groove of DNA.
authors.affiliation: Department of Chemistry, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey. Electronic address: rgup@mu.edu.tr.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Gup
authors.affiliation: Department of Chemistry, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Gökçe
authors.affiliation: Department of Physics, Arts and Sciences Faculty Aksaray University, 68100 Aksaray, Turkey.
authors.forename: Nefise
authors.initials: N
authors.lastname: Dilek
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D003851:Deoxyribonucleases / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008958:Models, Molecular; D008968:Molecular Conformation; D011725:Pyridines / Q000737:chemistry*; D014783:Viscosity; D015032:Zinc / Q000737:chemistry*
chemical_list: D006843:Hydrocarbons, Chlorinated; D011725:Pyridines; D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases; D015032:Zinc; D019342:Acetic Acid