title: Protein adsorption on a laser-modified titanium implant surface.
abstract: OBJECTIVE
  The aim of this study was to investigate the earlier phase of the osseointegration of a laser-treated implant surface in terms of human protein adsorption.
  
  
  METHODS
  Titanium surfaces were divided into machined (M), sandblasted (SB), and laser-treated (LT). The LT surfaces were created with an Nd diode-pumped laser in Q-switching, whereas the SB were treated with Al2O3. An x-ray photoelectron spectroscopy (XPS) analysis of titanium surface was performed. Titanium discs were used for albumin and fibronectin adsorption evaluation through fluorescence intensity. Fibronectin evaluation was also made with Western Blot analysis on experimental implants.
  
  
  RESULTS
  LT discs appeared to trigger a higher albumin and fibronectin adsorption with a regular pattern. The mean count of albumin adsorption was 0.29 and 3.8 for SB and LT, respectively (P = 0.016), whereas fibronectin values were 0.67 and 4.9 for (SB) and (LT) titanium (P = 0.02). XPS analysis showed that titanium, oxygen, carbon, and nitrogen were found on all 3 surfaces.
  
  
  CONCLUSIONS
  Laser-engineered porous titanium surface seems to promote, in vitro, the adsorption of albumin and fibronectin more than sandblasted (SB) or machined (M) implants.
authors.affiliation: *Junior Researcher, Unit of Dentistry and Oral Surgery, Department of Surgical and Medical Pathology, University of Pisa, Pisa, Italy. †PhD Student, Unit of Dentistry and Oral Surgery, Department of Surgical and Medical Pathology, University of Pisa, Pisa, Italy. ‡Biologist, Istituto di Genomica Applicata, Udine, Italy. §Assistant Professor, Unit of Dentistry and Oral Surgery, Department of Surgical and Medical Pathology, University of Pisa, Pisa, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cei
authors.forename: Dimitra
authors.initials: D
authors.lastname: Karapetsa
authors.forename: Emanuela
authors.initials: E
authors.lastname: Aleo
authors.forename: Filippo
authors.initials: F
authors.lastname: Graziani
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000378:metabolism*; D015153:Blotting, Western; D015921:Dental Implants; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D016348:Osseointegration; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014025:Titanium
chemical_list: D000418:Albumins; D015921:Dental Implants; D005353:Fibronectins; D014025:Titanium