title: Bacillus crassostreae sp. nov., isolated from an oyster (Crassostrea hongkongensis).
abstract: A novel Gram-stain-positive, motile, catalase- and oxidase-positive, endospore-forming, facultatively anaerobic rod, designated strain JSM 100118(T), was isolated from an oyster (Crassostrea hongkongensis) collected from the tidal flat of Naozhou Island in the South China Sea. Strain JSM 100118(T) was able to grow with 0-13% (w/v) NaCl (optimum 2-5%), at pH 5.5-10.0 (optimum pH 7.5) and at 5-50 °C (optimum 30-35 °C). The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant respiratory quinone was menaquinone-7 and the major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 and C16 : 1ω11c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown glycolipid and an unknown phospholipid. The genomic DNA G+C content was 35.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 100118(T) belonged to the genus Bacillus , and was most closely related to Bacillus litoralis SW-211(T) (98.9% 16S rRNA gene sequence similarity), Bacillus halosaccharovorans E33(T) (98.3%), Bacillus niabensis 4T19(T) (97.8%) and Bacillus herbersteinensis D-1,5a(T) (97.1%). The combination of results from the phylogenetic analysis, DNA-DNA hybridization, and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 100118(T) represents a novel species of the genus Bacillus , for which the name Bacillus crassostreae sp. nov. is proposed. The type strain is JSM 100118(T) ( = CTCC AB 2010452(T) =DSM 24486(T) =JCM 17523(T)).
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, 416000 Jishou, PR China.|Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization, Hunan Agricultural University, 410128 Changsha, PR China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Chen
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, 416000 Jishou, PR China.
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Tian
authors.affiliation: Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization, Hunan Agricultural University, 410128 Changsha, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yang
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, 416000 Jishou, PR China.
authors.forename: Ze-Qiang
authors.initials: ZQ
authors.lastname: He
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
authors.forename: Huazhong
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, 416000 Jishou, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
mesh_terms: D000818:Animals; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D049896:Crassostrea / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7