title: Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis.
abstract: The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Juliana L
authors.initials: JL
authors.lastname: Dreyfuss
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Caio V
authors.initials: CV
authors.lastname: Regatieri
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Coelho
authors.affiliation: Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: José B
authors.initials: JB
authors.lastname: Barbosa
authors.affiliation: Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Denise
authors.initials: D
authors.lastname: De Freitas
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: João R
authors.initials: JR
authors.lastname: Martins
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000503:physiopathology; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D003236:Conjunctivitis, Viral / Q000175:diagnosis* / Q000503:physiopathology / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis*; D008297:Male; D012680:Sensitivity and Specificity; D013666:Tears / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D005136:Eye Proteins; C042434:tear proteins; D006820:Hyaluronic Acid