title: Diagnostic performance of a rapid in-clinic test for the detection of Canine Parvovirus under different storage conditions and vaccination status.
abstract: Canine parvovirus (CPV) is one of the most common causes of acute haemorrhagic enteritis in young dogs, while clinical diagnosis is often indecisive. The aim of our study was to evaluate the diagnostic accuracy of an in-clinic rapid test in the detection of CPV infection in dogs. To this end, we compared the Rapid Diagnostic Kit of Canine Parvovirus, Coronavirus and Rotavirus antigen (Quicking(®)) to PCR, which is considered as the most reliable diagnostic method. A total of 78 duplicated faecal samples were collected from diarrhoeic dogs. Vaccination history within a month prior to the onset of diarrhoea was reported for 12 of the sampled dogs. The rapid diagnostic test was performed in 23 of the faecal samples directly, while the rest were placed into a sterile cotton tipped swab suitable for collection and transportation of viruses (Sigma Σ-VCM(®)) and stored at -20 °C. The sensitivity of the Quicking rapid diagnostic test compared to PCR in the total number of samples, in samples from non-vaccinated dogs and in samples tested directly after collection were 22.22% (95% CI: 13.27-33.57%), 26.67% (95% CI: 16.08-39.66%) and 76.47% (95% CI: 50.10-93.04%) respectively, while the specificity of the test was 100% in any case. In conclusion, negative results do not exclude parvoenteritis from the differential diagnosis, especially in dogs with early vaccination history, but a positive result almost certainly indicates CPV infection. An improved sensitivity may be expected when the test is performed immediately.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Greece.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Kantere
authors.affiliation: Department of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Greece. Electronic address: lathan@vet.uth.gr.
authors.forename: Labrini V
authors.initials: LV
authors.lastname: Athanasiou
authors.affiliation: Department of Animal Production, Technological and Educational Institute, Larissa, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Spyrou
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: Constantinos S
authors.initials: CS
authors.lastname: Kyriakis
authors.affiliation: Department of Veterinary Public Health, National School of Public Health, Athens, Greece.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Kontos
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Greece.
authors.forename: Dimitrios C
authors.initials: DC
authors.lastname: Chatzopoulos
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Greece.
authors.forename: Constantina N
authors.initials: CN
authors.lastname: Tsokana
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Greece.
authors.forename: Charalambos
authors.initials: C
authors.lastname: Billinis
mesh_terms: D000818:Animals; D003955:Diagnostic Tests, Routine / Q000379:methods*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005243:Feces / Q000821:virology*; D005615:Freezing; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
keywords: Canine parvovirus; Method comparison; Rapid in-clinic diagnostic test