title: Backbone resonance assignments of the PRYSPRY domain of TRIM25.
abstract: TRIM25 is a member of the tripartite motif (TRIM) family and has been implicated in the regulation of innate immune signaling via the RIG-I (retinoic acid-inducible gene-I) pathway for antiviral defense. As the essential first step towards the structural and functional characterization of the TRIM25/RIG-I interaction, the backbone resonance of the PRYSPRY domain of TRIM25 is assigned here based on triple-resonance experiments using uniformly [(2)H, (13)C, (15)N]-labeled protein.
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Kong
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Srinivasa R
authors.initials: SR
authors.lastname: Penumutchu
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Hung
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian, People's Republic of China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian, People's Republic of China. twlin@xmu.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Lin
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan. cyu.nthu@gmail.com.
authors.forename: Chin
authors.initials: C
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D014157:Transcription Factors / Q000737:chemistry*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry*
chemical_list: D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; C084523:TRIM25 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: NMR; PRYSPRY; RIG-1; Resonance assignment; TRIM25; β-Strands