title: Cytological assessment of the epithelial cells of the nasal mucous membrane after local fluticasone therapy.
abstract: The majority of cytological studies concern the influence of glucocorticosteroids on cells involved in creating and sustaining inflammation, such as eosinophils or neutrophils. Much less attention is devoted to epithelial cells. It should also be noticed that glucocorticosteroid drugs administered nasally for local action can significantly change the cytological image of the nasal mucous membrane. Therefore, the purpose of this research was to cytologically assess the influence of topical fluticasone therapy on the nasal mucous membrane cells, with special attention for the changes in the morphology of epithelial cells. The research samples were taken from patients with symptoms of chronic rhinitis and suspected allergies. The research was a two-step process. In the first step, a smear was taken from the surface of the nasal mucous membrane of the above-mentioned patients before the start of therapy and the obtained cytological image was compared with a control image of the nasal mucous of healthy people. Step two involved the cytology of the same patients after 4 weeks of fluticasone therapy, applied as a nasal aerosol in two doses of 50 μg to each nostril once per day, in the combined daily dose of 200 μg (for adults and children aged 12 or more). Children aged between 4 and 12 were given a single dose of 50 μg to each nostril once per day, in a daily dose of 100 μg. Based on smears stained according to the Papanicolaou and Pappenheim method, a qualitative and quantitative analysis of changes in the mucous membrane of nasal cells was performed. The cytological assessment of nasal mucous membrane stains of patients with chronic rhinitis before fluticasone treatment enabled a diagnosis of chronic infectious rhinitis, compared through the presence of numerous neutrophils and bacteria. The studied samples did not show significant changes in the morphology of epithelial cells, only a few cells with mild vacuolation changes of the cytoplasm were found. The use of fluticasone, however, caused a significant decrease in the neutrophilia and the appearance of numerous epithelial cells with intensified cytoplasm vacuolation in the sample. The results obtained allow us to conclude that standard fluticasone therapy as administered nasally in aerosol form to patients with diagnosed nonallergic nasal mucous membrane inflammation caused a significant reduction in the inflammation without showing cytological characteristics of damage to the epithelium of the nasal mucous membrane. The intensified vacuolation observed in the cytoplasm of epithelial cells, most prominently in the columnar cells, might suggest the stimulation of autophagic processes.
authors.affiliation: Department of Cell Biology and Electron Microscopy, Jan Kochanowski University, Kielce, Poland. tkrol@op.pl.
authors.forename: E
authors.initials: E
authors.lastname: Trybus
authors.forename: T
authors.initials: T
authors.lastname: Krol
authors.forename: T
authors.initials: T
authors.lastname: Obarzanowski
authors.forename: W
authors.initials: W
authors.lastname: Trybus
authors.forename: A
authors.initials: A
authors.lastname: Kopacz-Bednarska
authors.forename: M
authors.initials: M
authors.lastname: Obarzanowski
mesh_terms: D000281:Administration, Intranasal; D000293:Adolescent; D000328:Adult; D000336:Aerosols; D018926:Anti-Allergic Agents / Q000008:administration & dosage* / Q000009:adverse effects; D001343:Autophagy / Q000187:drug effects; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004334:Drug Administration Schedule; D004847:Epithelial Cells / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D005260:Female; D000068298:Fluticasone / Q000008:administration & dosage* / Q000009:adverse effects; D006801:Humans; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D020556:Neutrophil Infiltration / Q000187:drug effects; D065006:Papanicolaou Test; D065631:Rhinitis, Allergic / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology; D013997:Time Factors; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D000336:Aerosols; D018926:Anti-Allergic Agents; D000068298:Fluticasone