title: Gammaherpesvirus small noncoding RNAs are bifunctional elements that regulate infection and contribute to virulence in vivo.
abstract: Many viruses express noncoding RNAs (ncRNAs). The gammaherpesviruses (γHVs), including Epstein-Barr virus, Kaposi's sarcoma-associated herpesvirus, and murine γHV68, each contain multiple ncRNA genes, including microRNAs (miRNAs). While these ncRNAs can regulate multiple host and viral processes in vitro, the genetic contribution of these RNAs to infection and pathogenesis remains largely unknown. To study the functional contribution of these RNAs to γHV infection, we have used γHV68, a small-animal model of γHV pathogenesis. γHV68 encodes eight small hybrid ncRNAs that contain both tRNA-like elements and functional miRNAs. These genes are transcribed by RNA polymerase III and are referred to as the γHV68 TMERs (tRNA-miRNA-encoded RNAs). To determine the total concerted genetic contribution of these ncRNAs to γHV acute infection and pathogenesis, we generated and characterized a recombinant γHV68 strain devoid of all eight TMERs. TMER-deficient γHV68 has wild-type levels of lytic replication in vitro and normal establishment of latency in B cells early following acute infection in vivo. In contrast, during acute infection of immunodeficient mice, TMER-deficient γHV68 has reduced virulence in a model of viral pneumonia, despite having an enhanced frequency of virus-infected cells. Strikingly, expression of a single viral tRNA-like molecule, in the absence of all other virus-encoded TMERs and miRNAs, reverses both attenuation in virulence and enhanced frequency of infected cells. These data show that γHV ncRNAs play critical roles in acute infection and virulence in immunocompromised hosts and identify these RNAs as a new potential target to modulate γHV-induced infection and pathogenesis.
  
  
  OBJECTIVE
  The gammaherpesviruses (γHVs) are a subfamily of viruses associated with chronic inflammatory diseases and cancer, particularly in immunocompromised individuals. These viruses uniformly encode multiple types of noncoding RNAs (ncRNAs) that are not translated into proteins. It remains unclear how virus-expressed ncRNAs influence the course and outcome of infection in vivo. Here, we generated a mouse γHV that lacks the expression of multiple ncRNAs. Notably, this mutant virus is critically impaired in the ability to cause disease in immunocompromised hosts yet shows a paradoxical increase in infected cells early during infection in these hosts. While the original mouse virus encodes multiple ncRNAs, the expression of a single domain of one ncRNA can partially reverse the defects of the mutant virus. These studies demonstrate that γHV ncRNAs can directly contribute to virus-induced disease in vivo and that these RNAs may be multifunctional, allowing the opportunity to specifically interfere with different functional domains of these RNAs.
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Diebel
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Oko
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Medina
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Brian F
authors.initials: BF
authors.lastname: Niemeyer
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Cody J
authors.initials: CJ
authors.lastname: Warren
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Claypool
authors.affiliation: Department of Molecular Genetics & Microbiology, University of Florida College of Medicine, Gainesville, Florida, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Tibbetts
authors.forename: Carlyne D
authors.initials: CD
authors.lastname: Cool
authors.affiliation: Department of Anesthesiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
authors.forename: Eric T
authors.initials: ET
authors.lastname: Clambey
authors.affiliation: Department of Immunology & Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA Linda.VanDyk@ucdenver.edu.
authors.forename: Linda F
authors.initials: LF
authors.lastname: van Dyk
mesh_terms: D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000502:physiology / Q000821:virology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006566:Herpesviridae Infections / Q000473:pathology / Q000821:virology; D054884:Host-Pathogen Interactions; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D018144:Rhadinovirus / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D022661:RNA, Untranslated; D037521:Virulence Factors