title: Development of imidazoline-2-thiones based two-photon fluorescence probes for imaging hypochlorite generation in a co-culture system.
abstract: We designed and prepared the imidazoline-2-thione containing OCl(-) probes, PIS and NIS, which operate through specific reactions with OCl(-) that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl(-) generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl(-) in live cells and tissues, indicating that this probe could have wide biological applications.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750 (Korea).
authors.forename: Qingling
authors.initials: Q
authors.lastname: Xu
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.forename: Gyoungmi
authors.initials: G
authors.lastname: Kim
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006624:Hippocampus / Q000187:drug effects; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis*; D048288:Imidazolines / Q000737:chemistry; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D017785:Photons; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013871:Thiones / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D048288:Imidazolines; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D013871:Thiones; D006997:Hypochlorous Acid; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate
keywords: co-culture systems; fluorescence probes; hypochlorite generation; two-photon microscopy