title: SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides.
abstract: An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme was fused to a highly soluble SUMO domain, in addition to being produced in an unfused form by a cold-shock expression system. Yields up to ∼325 mg/L(-1) were achieved in batch cultivations. The 6x-His-tagged enzyme was purified employing an Ni(2+)-IMAC-based procedure. Identity of the protein was established by Western Blot analysis as well as peptide mass fingerprinting. A molecular mass of 64 kDa and an isoelectric point of pI 4.95 were determined by 2D gel electrophoresis. Cleavage of the fusion domain was possible by digestion with specific SUMO protease. The synthase was active in Mg(2+) containing buffer and catalyzed the production of (+)-zizaene (syn. khusimene), a precursor of khusimol, from farnesyl diphosphate. Product identity was confirmed by GC-MS and comparison of retention indices. Enzyme kinetics were determined by measuring initial reaction rates for the product, using varying substrate concentrations. By assuming a Michaelis-Menten model, kinetic parameters of KM = 1.111 μM (±0.113), vmax = 0.3245 μM min(-1) (±0.0035), kcat = 2.95 min(-1), as well as a catalytic efficiency kcat/KM = 4.43 × 10(4) M(-1)s(-1) were calculated. Fusion to a SUMO moiety can substantially increase soluble expression levels of certain hard to express terpene synthases in E. coli. The kinetic data determined for the recombinant synthase are comparable to other described plant sesquiterpene synthases and in the typical range of enzymes belonging to the secondary metabolism. This leaves potential for optimizing catalytic parameters through methods like directed evolution.
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hartwig
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Frister
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Alemdar
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Scheper
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany. Electronic address: beutel@iftc.uni-hannover.de.
authors.forename: S
authors.initials: S
authors.lastname: Beutel
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D049930:Chrysopogon / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D000081223:Polycyclic Sesquiterpenes; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D025842:SUMO-1 Protein / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D000081223:Polycyclic Sesquiterpenes; D011106:Polyisoprenyl Phosphates; D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein; D012717:Sesquiterpenes; C091305:khusimol; C004808:farnesyl pyrophosphate; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: Enzyme kinetics; Khusimene; SUMO; Sesquiterpenes; Terpene synthase; Zizaene