title: Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.
abstract: Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater.
authors.affiliation: Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka, 565-0871, Japan, matsushima-k@phs.osaka-u.ac.jp.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Matsushima
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kaneda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Hirata
mesh_terms: D001559:Benzhydryl Compounds / Q000378:metabolism*; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology*; D031822:Portulaca / Q000201:enzymology*; D013696:Temperature; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002457:Cell Extracts; D004800:Enzymes, Immobilized; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds