title: Development of 5Ns chromosome-specific SCAR markers for utilization in future wheat breeding programs.
abstract: In previous studies, we developed a wheat-Psathyrostachys huashanica Keng disomic addition line 3-8-10-2, which exhibited high stripe rust resistance and could be used as a donor source for introducing novel disease resistance gene(s) into wheat in future breeding programs. It was identified using cytology, genomic in situ hybridization (GISH), EST-SSR, EST-STS and morphological analyses. However, these techniques are not suitable for breeding programs that require the rapid screening of large numbers of genotypes because they are highly technical and time-consuming. In this study, three Ns genome-specific SCAR markers were developed via random amplified polymorphic DNA (RAPD) markers. These SCAR markers were further validated using a complete set of wheat-P. huashanica disomic addition lines, which segregated the 5Ns disomic addition line individuals. Our results indicated that the SCAR markers associated with the 5Ns chromosome of P. huashanica and they provide a low cost, high efficiency, alternative tool for screening 5Ns chromosomes in a wheat background. These newly developed SCAR markers that species-specificity of the markers was proved by analysis of a wide range of cereal species, and specific for 5Ns chromosome, which should be useful in marker-assisted selection for wheat breeders who want to screen genotypes that may contain 5Ns chromatin.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: L M
authors.initials: LM
authors.lastname: Wang
authors.forename: W L
authors.initials: WL
authors.lastname: Du
authors.forename: L G
authors.initials: LG
authors.lastname: Chen
authors.forename: S H
authors.initials: SH
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: J X
authors.initials: JX
authors.lastname: Zhao
authors.forename: Q H
authors.initials: QH
authors.lastname: Yang
authors.forename: X H
authors.initials: XH
authors.lastname: Chen
mesh_terms: D001947:Breeding / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics*; D005819:Genetic Markers; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers