title: Effect of Pre-Stressing on the Acid-Stress Response in Bifidobacterium Revealed Using Proteomic and Physiological Approaches.
abstract: Weak acid resistance limits the application of Bifidobacteria as a probiotic in food. The acid tolerance response (ATR), caused by pre-stressing cells at a sublethal pH, could improve the acid resistance of Bifidobacteria to subsequent acid stress. In this study, we used Bifidobacterium longum sub. longum BBMN68 to investigate the effect of the ATR on the acid stress response (ASR), and compared the difference between the ATR and the ASR by analyzing the two-dimensional-PAGE protein profiles and performing physiological tests. The results revealed that a greater abundance of proteins involved in carbohydrate metabolism and protein protection was present after the ASR than after the ATR in Bifidobacterium. Pre-stressing cells increased the abundance of proteins involved in energy production, amino acid metabolism, and peptidoglycan synthesis during the ASR of Bifidobacterium. Moreover, after the ASR, the content of ATP, NH3, thiols, and peptidoglycan, the activity of H+-ATPase, and the maintenance of the intracellular pH in the pre-stressed Bifidobacterium cells was significantly higher than in the uninduced cells. These results provide the first explanation as to why the resistance of Bifidobacterium to acid stress improved after pre-stressing.
authors.affiliation: Food Science and Engineering College, Beijing University of Agriculture, Beijing Laboratory for Food Quality and Safety, Beijing, 102206, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Jin
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qin
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China; Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, 100083, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China; Key Laboratory for Functional Dairy, China Agriculture University, Beijing, 100083, China.
authors.forename: Songling
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China; Beijing Resources Yatai Feed Technology Co., LTD, Beijing, China.
authors.forename: Shaoyang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Food Science and Engineering College, Beijing University of Agriculture, Beijing Laboratory for Food Quality and Safety, Beijing, 102206, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Cui
authors.affiliation: College of Food Science and Nutritional Engineering, China Agriculture University, Beijing, 100083, China.
authors.forename: Fazheng
authors.initials: F
authors.lastname: Ren
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001644:Bifidobacterium / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000737:chemistry; D010457:Peptidoglycan / Q000096:biosynthesis; D040901:Proteomics; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013312:Stress, Physiological; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D010457:Peptidoglycan; D013438:Sulfhydryl Compounds; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases