title: 3-Deoxyglucosone: a potential glycating agent accountable for structural alteration in H3 histone protein through generation of different AGEs.
abstract: Advanced glycation end-products (AGEs) are heterogeneous group of compounds, known to be implicated in diabetic complications. One of the consequences of the Maillard reaction is attributed to the production of reactive intermediate products such as α-oxoaldehydes. 3-deoxyglucosone (3-DG), an α-oxoaldehyde has been found to be involved in accelerating vascular damage during diabetes. In the present study, calf thymus histone H3 was treated with 3-deoxyglucosone to investigate the generation of AGEs (Nε-carboxymethyllysine, pentosidine), by examining the degree of side chain modifications and formation of different intermediates and employing various physicochemical techniques. The results clearly indicate the formation of AGEs and structural changes upon glycation of H3 by 3-deoxyglucosone, which may hamper the normal functioning of H3 histone, that may compromise the veracity of chromatin structures and function in secondary complications of diabetes.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Jalaluddin M
authors.initials: JM
authors.lastname: Ashraf
authors.affiliation: Department of Biotechnology, Integral University, Lucknow, India.
authors.forename: Saheem
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Gulam
authors.initials: G
authors.lastname: Rabbani
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Qambar
authors.initials: Q
authors.lastname: Hasan
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Arif Tasleem
authors.initials: AT
authors.lastname: Jan
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Rizwan Hasan
authors.initials: RH
authors.lastname: Khan
authors.affiliation: Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Khursheed
authors.initials: K
authors.lastname: Alam
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
mesh_terms: D000818:Animals; D002417:Cattle; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D048909:Diabetes Complications / Q000209:etiology / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D006657:Histones / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced; D006657:Histones; D003847:Deoxyglucose; C016350:3-deoxyglucosone