title: Expression profiling analysis of the microRNA response of Cynoglossus semilaevis to Vibrio anguillarum and other stimuli.
abstract: To investigate the roles of microRNAs (miRNA) of Cynoglossus semilaevis in response to Vibrio anguillarum that were previously identified using high-throughput sequencing, microarray analyses was performed on three small RNA libraries (CG, NOSG, and HOSG) prepared from C. semilaevis immune tissues. In total, of 1279 designed probes, 739 (57.78 %) were detectable. The expression levels of these miRNAs were analyzed using pairwise comparisons among the three libraries, and a total of 99 miRNAs were observed to be significantly differentially expressed. The expression patterns of 10 differentially expressed miRNAs were validated by real-time quantitative PCR (RT-qPCR). In addition, expression of miR-142-5p, miR-223, and miR-181a in response to V. anguillarum at numerous time-points in four tissues, as well as the responses to lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), peptidoglycan (PGN), and red-spotted grouper nervous necrosis virus (RGNNV) in head kidney cells, were studied by qRT-PCR. Taken together, all of the expression profiles showed significant differences compared to the control group; both similarities and differences in the expression responses to the same pathogen were observed. Collectively, these findings highlighted the putative roles for miRNAs in the context of the innate immune response of C. semilaevis exposing to pathogens and that further studies are needed to understand the molecular mechanisms of miRNA regulation in C. semilaevis host-pathogen interactions.
authors.affiliation: Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, People's Republic of China.
authors.forename: Guangye
authors.initials: G
authors.lastname: Gong
authors.forename: Zhenxia
authors.initials: Z
authors.lastname: Sha
authors.forename: Songlin
authors.initials: S
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Yadong
authors.initials: Y
authors.lastname: Chen
authors.forename: Tianzi
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D005412:Flatfishes / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D060226:Head Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000472:pathogenicity / Q000502:physiology; D010457:Peptidoglycan / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D012327:RNA Virus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D014733:Vibrio / Q000472:pathogenicity / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*
chemical_list: D008070:Lipopolysaccharides; D035683:MicroRNAs; D010457:Peptidoglycan; D011070:Poly I-C