title: Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents.
abstract: The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this assay, unlike those of the [(3)H]hypoxanthine incorporation assay, for the antimalarial activity of the above DNA binding agents may be erroneous. In the case of CPPs, false readouts can be improved by the removal of excess peptides.
authors.affiliation: Cell Biology and Immunology Division, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Bhatia
authors.affiliation: Bioinformatics Centre, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Ankur
authors.initials: A
authors.lastname: Gautam
authors.affiliation: Cell Biology and Immunology Division, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Shailendra K
authors.initials: SK
authors.lastname: Gautam
authors.affiliation: Cell Biology and Immunology Division, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Mehta
authors.affiliation: Cell Biology and Immunology Division, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Bioinformatics Centre, CSIR-Institute of Microbial Technology, Chandigarh, India grish@imtech.res.in raghava@imtech.res.in.
authors.forename: Gajendra P S
authors.initials: GP
authors.lastname: Raghava
authors.affiliation: Cell Biology and Immunology Division, CSIR-Institute of Microbial Technology, Chandigarh, India grish@imtech.res.in raghava@imtech.res.in.
authors.forename: Grish C
authors.initials: GC
authors.lastname: Varshney
mesh_terms: D000962:Antimalarials / Q000737:chemistry*; D052160:Benzothiazoles; D001681:Biological Assay / Q000379:methods*; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D003959:Diamines; D005453:Fluorescence; D007364:Intercalating Agents / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D011804:Quinolines
chemical_list: D000962:Antimalarials; D052160:Benzothiazoles; D057846:Cell-Penetrating Peptides; D003959:Diamines; D007364:Intercalating Agents; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA