title: [The contribution of different alpha-amylase isoenzymes of the commodity grain spring wheat in the formation of falling number values].
abstract: The participation of various isoenzymes of alpha-amylase in the formation of falling number values of the commodity grain of wheat grown in the Republic of Kazakhstan was investigated. It was found that active isoenzymes alpha-AMY1 and alpha-AMY2 of the embryonic shield were present in the grain with an index over 200. A significant decrease in the falling number depended mainly on the synthesis of alpha-AMY1 and alpha-AMY2 isoenzymes in the aleurone layer. In the grain, isoenzymes with high isoelectric points (p1 > or = 7.3) were found; these isoenzymes belong to alpha-amylase or late maturing or alpha-amylase of practically mature grains. It was discovered that the exogenous hormone (gibberellic acid) induced synthesis of alpha-amylase isoenzymes of scutellum, whole caryopses, and aleurone. It was shown that the impact of exogenous gibberellic acid on the activity and structure of alpha-amylase is reduced in grain with a low falling number.
authors.forename: N S
authors.initials: NS
authors.lastname: Mamytova
authors.forename: V A
authors.initials: VA
authors.lastname: Kuzovlev
authors.forename: A A
authors.initials: AA
authors.lastname: Khakimzhanov
authors.forename: O V
authors.initials: OV
authors.lastname: Fursov
mesh_terms: D001939:Bread / Q000032:analysis; D002523:Edible Grain / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D005875:Gibberellins / Q000378:metabolism / Q000494:pharmacology; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D007623:Kazakhstan; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005875:Gibberellins; D007527:Isoenzymes; D010937:Plant Growth Regulators; D010940:Plant Proteins; C007842:gibberellic acid; D000516:alpha-Amylases