title: [Isolation and identification of electrochemically active microorganism from micro-aerobic environment].
abstract: Extracellular electron transfer of electrochemically active microorganism plays vital role in biogeochemical cycling of metals and carbon and in biosynthesis of bioenergy. Compared to anaerobic anode, micro-aerobic anode captures more energy from microbial fuel cell. However, most of previous researches focused on functioning bacteria in anaerobic anode, functioning bacteria in micro-aerobic anode was rarely studied. Herein, we used the traditional aerobic screening technology to isolate functioning bacteria from a micro-aerobic anode. Three pure cultures Aeromonas sp. WS-XY2, Citrobacter sp. WS-XY3 and Bacterium strain WS-XY4 were obtained. WS-XY2 and WS-XY3 were belonged to Proteobacteria, whereas WS-XY4 was possibly a new species. Cyclic voltammetry and chronoamperometry analysis demonstrated all of them showed the electrochemical activity by direct extracellular electron transfer, and micro-aerobic anode could select bacteria that have similar electrochemical activity to proliferate on the anode. We further conclude that functioning bacteria in micro-aerobic anode are more efficient than that of anaerobic anode may be the reason that micro-aerobic anode has better performance than anaerobic anode. Therefore, a thorough study of functioning bacteria in micro-aerobic anode will significantly promote the energy recovery from microbial fuel cell.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004566:Electrodes; D004579:Electron Transport