title: Influence of long-term fertilization on soil microbial biomass, dehydrogenase activity, and bacterial and fungal community structure in a brown soil of northeast China.
abstract: In this study, the effect of mineral fertilizer and organic manure were evaluated on soil microbial biomass, dehydrogenase activity, bacterial and fungal community structure in a long-term (33 years) field experiment. Except for the mineral nitrogen fertilizer (N) treatment, long-term fertilization greatly increased soil microbial biomass carbon (SMBC) and dehydrogenase activity. Organic manure had a significantly greater impact on SMBC and dehydrogenase activity, compared with mineral fertilizers. Bacterial and fungal community structure was analyzed by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Long-term fertilization increased bacterial and fungal ribotype diversity. Total soil nitrogen (TN) and phosphorus (TP), soil organic carbon (SOC) and available phosphorus (AP) had a similar level of influence on bacterial ribotypes while TN, SOC and AP had a larger influence than alkali-hydrolyzable nitrogen (AHN) on fungal ribotypes. Our results suggested that long-term P-deficiency fertilization can significantly decrease soil microbial biomass, dehydrogenase activity and bacterial diversity. N-fertilizer and SOC have an important influence on bacterial and fungal communities.
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China ; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Luo
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China ; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Xiaori
authors.initials: X
authors.lastname: Han
authors.affiliation: Foreign Language Teaching Department, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China ; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China ; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Dongling Road 120, Shenyang, 110866 Liaoning China ; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Dongling Road 120, Shenyang, 110866 Liaoning China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Bai
keywords: Bacterial and fungal community structure; Dehydrogenase activity; Long-term fertilization; PCR-DGGE; Soil microbial biomass carbon