title: Effect of IL-18 binding protein on hepatic ischemia-reperfusion injury induced by infrarenal aortic occlusion.
abstract: OBJECTIVE
  Severe local and systemic tissue damage called ischemia/reperfusion (IR) injury occurs during the period of reperfusion. Free oxygen radicals and proinflammatory cytokines are responsible for reperfusion injury. IL-18 binding protein (IL-18BP) is a natural inhibitor of IL-18. The balance between IL-18 and IL-18BP has an important role in the inflammatory setting. The present study aimed to investigate whether IL-18BP had a protective role in remote organ hepatic IR injury.
  
  
  METHODS
  Wistar-Albino rats were divided into three groups that contained seven rats. Group I (sham): Laparotomy and infrarenal abdominal aorta (AA) dissection were done but no clamping was done. Group II (I/R): The infrarenal AA was clamped by atraumatic microvascular clamp for 30 minutes and then was exposed to 90 minutes of reperfusion. Group III (IR + IL-18BP): 75 µg/kg of IL-18BP in 0.9% saline (1 mL) was administered 30 minutes before infrarenal AA dissection and clamping; 30 minutes of ischemia was applied and then was exposed to 90 minutes of reperfusion.
  
  
  RESULTS
  Serum AST, ALT, and LDH levels were remarkably higher in IR group and returned to normal levels in treatment group. The proinflammatory cytokine levels had decreased in treatment group, and was statistically significant compared with the IR group. Serum levels of total oxidant status and oxidative stress index decreased and levels of total antioxidant status increased by IL-18BP.
  
  
  CONCLUSIONS
  This study suggested that IL-18BP has antioxidant, anti-inflammatory and hepatoprotective effects in cases of IR with infrarenal AA induced liver oxidative damage.
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozsoy
authors.affiliation: Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Yucel
authors.initials: Y
authors.lastname: Gonul
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bal
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ziya Taner
authors.initials: ZT
authors.lastname: Ozkececi
authors.affiliation: Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ruchan Bahadir
authors.initials: RB
authors.lastname: Celep
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Fahri
authors.initials: F
authors.lastname: Adali
authors.affiliation: Department of Biochemistry, Faculty of Arts And Sciences, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Hazman
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koçak
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Tosun
keywords: Acute liver injury; IL-18 binding protein; Interleukin-18; Reperfusion Injury; Transplantation