title: Enolase is regulated by Liver X Receptors.
abstract: Enolase is a glycolytic enzyme known to inhibit cholesteryl ester hydrolases (CEHs). Cholesteryl ester loading of macrophages, as occurs during atherosclerosis, is accompanied by increased Enolase protein and activity. Here, we describe that J774 macrophages treated with LXR agonists exhibit reduced Enolase transcript and protein abundance. Moreover, we show that this reduction is further potentiated by activation of the LXR/RXR heterodimer with the RXR ligand 9-cis retinoic acid. Enolase levels are also reduced in vivo following activation of LXRs in the intestine, but not in the liver. This effect is lost in Lxrαβ-/- mice. In aggregate, our study identified Enolase as a new target of LXRs in vivo, which may promote cholesterol mobilization for subsequent efflux.
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: De Boussac
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France; Service d'Endocrinologie, Diabétologie et Maladies Métaboliques, Hôpital Gabriel Montpied, F-63003 Clermont-Ferrand, France; Service de Médecine Nucléaire, Centre Jean Perrin, 58 rue Montalembert, F-63011 Clermont-Ferrand, France.
authors.forename: Salwan
authors.initials: S
authors.lastname: Maqdasy
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Amalia
authors.initials: A
authors.lastname: Trousson
authors.affiliation: Department of Medical Biochemistry Academic, Medical Center, Amsterdam 1105 AZ, The Netherlands.
authors.forename: Noam
authors.initials: N
authors.lastname: Zelcer
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: David H
authors.initials: DH
authors.lastname: Volle
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Jean-Marc A
authors.initials: JM
authors.lastname: Lobaccaro
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, BP 10448, F63000 Clermont-Ferrand, France; CNRS, UMR 6293, GReD, F-63177 Aubiere, France; INSERM, UMR 1103, GReD, F-63177 Aubiere, France; Centre de Recherche en Nutrition Humaine d'Auvergne, F-63000 Clermont-Ferrand, France. Electronic address: silvere.baron@univ-bpclermont.fr.
authors.forename: Silvère
authors.initials: S
authors.lastname: Baron
mesh_terms: D000818:Animals; D007422:Intestines / Q000201:enzymology; D000071518:Liver X Receptors; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008871:Microvilli / Q000201:enzymology; D008954:Models, Biological; D057093:Orphan Nuclear Receptors / Q000819:agonists / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D010751:Phosphopyruvate Hydratase
keywords: Cholesterol esters; Enolase; Liver X Receptors; Macrophages