title: [On specific properties of the mitochondrial oxidative phosphorylation system operating as a supercomplex].
abstract: This paper represents the study of endogenous and exogenous fatty acids affecting the mitochondrial phosphorylation system effectiveness depending on temperature. The experiment was set up under conditions in which the oxidative phosphorylation system operates as a supercomplex. Rat liver mitochondria were isolated without purposive fatty acids removal from membranes, then studied in hypotonic medium (120 mOsm). We managed to detect a very narrow interval 19 ± 1°C where the fatty acid uncoupling effect is weak up to disappearing. At the same small temperature range, a structural rearrangement that takes place in the enzyme system is accompanied with denser packing of membrane protein complexes. Thus, at the temperatures close to 19°C the supercomplex works in the specific regime protected (or partially protected) from the uncoupling effect of fatty acids. Here we also discuss a physiological significance of the increased ATP-synthesis effectiveness at lower temperatures and the most probable character of structural rearrangement taking place at 19°C in the enzymes in the mitochondrial membrane.
authors.forename: S V
authors.initials: SV
authors.lastname: Nesterov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Skorobogatova
authors.forename: L S
authors.initials: LS
authors.lastname: Iaguzhinskiĭ
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D008930:Mitochondria, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009097:Multienzyme Complexes; D000255:Adenosine Triphosphate