title: Lipase-catalyzed dissipative self-assembly of a thixotropic Peptide bolaamphiphile hydrogel for human umbilical cord stem-cell proliferation.
abstract: We report lipase-catalyzed inclusion of p-hydroxy benzylalcohol to peptide bolaamphiphiles. The lipase-catalyzed reactions of peptide bolaamphiphiles with p-hydroxy benzylalcohol generate dynamic combinatorial libraries (DCL) in aqueous medium that mimic the natural dissipative system. The peptide bolaamphiphile 1 (HO-WY-Suc-YW-OH) reacts with p-hydroxy benzylalcohol in the presence of lipase forming an activated diester building block. The activated diester building block self-assembles to produce nanofibrillar thixotropic hydrogel. The subsequent hydrolysis results in the dissipation of energy to form nonassembling bolaamphiphile 1 with collapsed nanofibers. The thixotropic DCL hydrogel matrix is used for 3D cell culture experiments for different periods of time, significantly supporting the cell survival and proliferation of human umbilical cord mesenchymal stem cells.
authors.affiliation: †Department of Chemistry, Indian Institute of Technology Indore, Khandwa Road, Indore 452017, India.
authors.forename: Apurba K
authors.initials: AK
authors.lastname: Das
authors.affiliation: †Department of Chemistry, Indian Institute of Technology Indore, Khandwa Road, Indore 452017, India.
authors.forename: Indrajit
authors.initials: I
authors.lastname: Maity
authors.affiliation: ‡Department of Biotechnology, Devi Ahilya Vishwavidyalaya, Khandwa Road, Indore 452001, India.
authors.forename: Hamendra S
authors.initials: HS
authors.lastname: Parmar
authors.affiliation: §Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZD, United Kingdom.
authors.forename: Tom O
authors.initials: TO
authors.lastname: McDonald
authors.affiliation: †Department of Chemistry, Indian Institute of Technology Indore, Khandwa Road, Indore 452017, India.
authors.forename: Maruthi
authors.initials: M
authors.lastname: Konda
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D049109:Cell Proliferation / Q000187:drug effects*; D005663:Furans / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008049:Lipase / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D058105:Polymerization; D011728:Pyridones / Q000737:chemistry*
chemical_list: D005663:Furans; D020100:Hydrogels; D010455:Peptides; D011728:Pyridones; C543575:bolaamphiphile; D008049:Lipase