title: Cerebral blood volume measurement using near-infrared time-resolved spectroscopy and histopathological evaluation after hypoxic-ischemic insult in newborn piglets.
abstract: The aim of this study was to assess the relationship between the cerebral blood volume (CBV) measured by near-infrared time-resolved spectroscopy (TRS) and pathological change of the brain in a hypoxic-ischemic (HI) piglet model. Twenty-one anesthetized newborn piglets, including three sham controls, were studied. An HI event was induced by low inspired oxygen. CBV was measured using TRS (Hamamatsu TRS-10). Data were collected before, during, and 6h after the insult. CBV was calculated as the change from the end of the insult. The piglets were allowed to recover from anesthesia for 6h after the insult. At the age of 5 days, the brains of the piglets were perfusion-fixed, and histologic evaluations of brain tissue were performed. The extent of histopathological damage was graded in 0.5-unit intervals on a 9-step scale. CBV increments were well correlated with histopathological scores, especially at 1 and 3h after resuscitation. Spearman's rank-correlation coefficients at 1, 3, and 6h after resuscitation in the gray matter were 0.9016, 0.9127, and 0.6907, respectively. We conclude that an increased CBV after HI insult indicates more marked histological brain damage. CBV measurement immediately after resuscitation provides a more precise prediction of the histological outcome.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan; Department of Neonatology, National Hospital Organization Okayama Medical Center, Okayama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Maternal Perinatal Center, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Jinnai
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hamano
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Maternal Perinatal Center, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Koyano
authors.affiliation: Departments of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Chiba
authors.affiliation: Maternal Perinatal Center, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kanenishi
authors.affiliation: Maternal Perinatal Center, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Saneyuki
authors.initials: S
authors.lastname: Yasuda
authors.affiliation: Departments of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.affiliation: Departments of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.affiliation: Departments of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hata
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kusaka
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure / Q000502:physiology; D001810:Blood Volume; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000098:blood supply* / Q000378:metabolism*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D004569:Electroencephalography; D005260:Female; D006339:Heart Rate / Q000502:physiology; D006454:Hemoglobins / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D019265:Spectroscopy, Near-Infrared; D013223:Statistics as Topic; D013552:Swine
chemical_list: D006454:Hemoglobins
keywords: Amplitude-integrated electroencephalography; Animal model; Brain histology; Cerebral blood volume; Hypoxia-ischemia; Near-infrared spectroscopy