title: Preservation of resin-dentin interfaces treated with benzalkonium chloride adhesive blends.
abstract: Reducing collagen degradation within hybrid layers may contribute to the preservation of adhesive interfaces. This study evaluated the stability of resin-dentin interfaces treated with benzalkonium chloride (BAC)-modified adhesive blends and assessed collagen degradation in dentin matrices treated with BAC. The etch-and-rinse adhesive, Adper Single Bond Plus, modified with 0.5% and 1.0% BAC, was evaluated for microtensile bond strength (μTBS) and nanoleakage (NL) after 24 h and 1 yr. Thirty completely demineralized dentin beams from human molars were dipped for 60 s in deionized water (DW; control), or in 0.5% or 1.0% BAC, and then incubated in simulated body fluid (SBF). Collagen degradation was assessed by quantification of the dry mass loss and the amount of hydroxyproline (HYP) released from hydrolyzed specimens after 1 or 4 wk. Although all groups demonstrated a significant increase in NL after 1 yr, adhesive modified with 0.5% BAC showed stable bond strength after 1 yr (9% decrease) relative to the control (44% decrease). Significantly less HYP release and dry mass loss were observed for both 0.5% and 1.0% BAC relative to the control. This in vitro study demonstrates that BAC contributes to the preservation of resin-dentin bonds for up to 1 yr by reducing collagen degradation.
authors.affiliation: Department of Restorative Dentistry, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA.
authors.forename: Camila
authors.initials: C
authors.lastname: Sabatini
authors.forename: Pilar A
authors.initials: PA
authors.lastname: Ortiz
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D001548:Benzalkonium Compounds / Q000737:chemistry*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002710:Chlorhexidine / Q000737:chemistry; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D001840:Dental Bonding; D003763:Dental Leakage / Q000145:classification; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D008422:Materials Testing; D010756:Phosphoric Acids / Q000737:chemistry; D011897:Random Allocation; D012995:Solubility; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D001548:Benzalkonium Compounds; D017220:Dentin-Bonding Agents; D010756:Phosphoric Acids; C511238:Single Bond Plus; D017438:Bisphenol A-Glycidyl Methacrylate; D003094:Collagen; C030242:phosphoric acid; D002710:Chlorhexidine; D006909:Hydroxyproline
keywords: benzalkonium chloride; bond strength; dental adhesive; matrix metalloproteinase; proteolysis