title: Isolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae).
abstract: •
  
  
  OBJECTIVE
  Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. •
  
  
  RESULTS
  Twenty-two primer pairs were developed from ESTs of T. dolabrata var. hondae. The primers amplified di- and trinucleotide repeat-containing sequences. Polymorphisms were assessed in 81 individuals from two T. dolabrata var. hondae populations and one T. dolabrata population. The number of alleles ranged from one to 17 per locus. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.926, respectively. •
  
  
  CONCLUSIONS
  These new EST-SSR markers will be useful in analyses of the genetic diversity and population structure of the genus Thujopsis.
authors.affiliation: Institute of Wood Technology, Akita Prefectural University, 11-1 Kaieisaka, Noshiro, Akita 016-0876, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Sato
authors.affiliation: Institute of Wood Technology, Akita Prefectural University, 11-1 Kaieisaka, Noshiro, Akita 016-0876, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Forestry and Forest Products Research Institute, Forest Tree Breeding Center, 3809-1 Ishi, Juo, Hitachi, Ibraraki 319-1301, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Mishima
authors.affiliation: Institute of Wood Technology, Akita Prefectural University, 11-1 Kaieisaka, Noshiro, Akita 016-0876, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takata
keywords: Cupressaceae; Thujopsis; expressed sequence tag; microsatellite; next-generation sequencing