title: Effect of ovarian hormones on maturation of dendritic cells from peripheral blood monocytes in dogs.
abstract: Previously, we reported that ovarian hormones affect the immune response against E. coli isolated from the dogs affected with pyometra. In order to investigate mechanisms underlying the immune modulation, we examined the effects of ovarian hormones on the generation of dendritic cells (DCs), the most potent antigen presenting cell. DCs were differentiated from peripheral blood monocytes (PBMOs) using a cytokine cocktail. Both estrogen receptor and progesterone receptors were expressed by the PBMOs and immature DCs. When various ovarian hormones were added to the culture for the DC differentiation, progesterone significantly decreased the expression of DC maturation markers, such as CD1a, CD80 and CD86, on mature DCs. Conversely, the addition of estrogen to the cultures increased the expression of CD86, but not other maturation makers. Furthermore, DCs differentiated in the presence of progesterone did not stimulate allogeneic mononuclear cells in PB. Taken together, these results indicate that progesterone diminishes the maturation of DCs, leading to decreased immune responses against invading pathogens.
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano-shi, Osaka 598-8531, Japan.
authors.forename: Viskam
authors.initials: V
authors.lastname: Wijewardana
authors.forename: Kikuya
authors.initials: K
authors.lastname: Sugiura
authors.forename: Daluthgamage Patsy H
authors.initials: DP
authors.lastname: Wijesekera
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.forename: Toshiya
authors.initials: T
authors.lastname: Nishimura
authors.forename: Ryoji
authors.initials: R
authors.lastname: Kanegi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ushigusa
authors.forename: Toshio
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007111:Immunity, Cellular / Q000187:drug effects / Q000502:physiology; D009000:Monocytes / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018949:Antigens, CD1; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C419393:CD1a antigen; D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone