title: [16β-hydroxylation of 4-androstene-3,17-dione by Aspergillus niger].
abstract: In order to discover the steroid biotransformation ability of filamentous fungus Aspergillus niger TCCC41650, we studied the fermentation of 4-androstene-3,17-dione with A. niger TCCC41650. The transformation product was purified, crystallized and determined as 16β-hydroxy-androst-4-ene-3,17-dione by X-ray single crystal diffraction method. The best fermentation condition was found to be pH 6.0, ethanol amount 2% with a substrate concentration of 1 per thousand, the transformation rate is 85.81% after 72 h. Based on the best of our knowledge, 16β-hydroxylation rarely occurs in microbial transformations of steroid. This study laid the foundation for the research of 16β-hydroxylation steroids
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Ge
authors.forename: Shuhong
authors.initials: S
authors.lastname: Mao
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
mesh_terms: D000735:Androstenedione / Q000378:metabolism*; D001234:Aspergillus niger / Q000378:metabolism*; D001711:Biotransformation; D005285:Fermentation; D006900:Hydroxylation; D007218:Industrial Microbiology
chemical_list: D000735:Androstenedione