title: Understanding of complex protein interactions with respect to anchorage independence.
abstract: Anchorage-independent growth of cells in soft agar is one of the hallmark characteristics of cellular transformation and uncontrolled cell growth. It may be considered as one of the most stringent assays for detecting malignant transformation of cells. Here, we describe a retroviral infection of a library of small secretory proteins and the use of the soft agar assay to obtain and study novel interacting protein combinations that cause cell transformation.
authors.affiliation: Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Proteos, Singapore, 138673, Singapore, mcbthamj@imcb.a-star.edu.sg.
authors.forename: Jill M
authors.initials: JM
authors.lastname: Tham
authors.forename: Sofie C
authors.initials: SC
authors.lastname: Van Huffel
authors.forename: Wanjin
authors.initials: W
authors.lastname: Hong
mesh_terms: D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D011506:Proteins; D011994:Recombinant Proteins