title: Downregulation of Survivin by shRNA Inhibits Invasion and Enhances the Radiosensitivity of Laryngeal Squamous Cell Carcinoma.
abstract: Radiotherapy is an important treatment modality for laryngeal carcinoma, however, some patients with early stage laryngeal carcinomas showed resistance to radiotherapy. Survivin overexpression is associated with radioresistance of laryngeal squamous cell carcinoma. We speculated that the inhibition of survivin expression might improve the radiosensitivity of laryngeal carcinoma. We assessed the effect of survivin expressions by shRNA on the radiosensitivity of laryngeal squamous cell carcinoma in vitro and in vivo. After transfection of shRNA-targeting survivin, MTS assay showed that the survival rates of radiation groups were reduced with the prolongation of culture time (p < 0.05). In addition, shRNA-targeting survivin abrogated radiation-induced G2 phase arrest and amplified radiation-induced apoptosis. In vivo, shRNA transfection also sensitized tumor xenograft to radiotherapy. Radioresistance of laryngeal squamous cell carcinoma may be associated with increased expression of survivin, and survivin suppression may enhance the radiosensitivity of laryngeal squamous cell carcinoma.
authors.affiliation: Department of Otorhinolaryngology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Pediatrics, Taihe Hospital Affiliated with Hubei University of Medicine, Shiyan, 442000, Hubei, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Oncology, Taihe Hospital Affiliated with Hubei University of Medicine, Shiyan, 442000, Hubei, People's Republic of China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Otorhinolaryngology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China. drzezhangtao@163.com.
authors.forename: Zezhang
authors.initials: Z
authors.lastname: Tao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000532:radiotherapy*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000532:radiotherapy*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000532:radiotherapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D034741:RNA, Small Interfering / Q000378:metabolism*; D011836:Radiation Tolerance; D000077195:Squamous Cell Carcinoma of Head and Neck; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin
keywords: Laryngeal squamous cell carcinoma; Radiosensitivity; Survivin