title: Simultaneous determination of eperisone hydrochloride and paracetamol in mouse plasma by high performance liquid chromatography-photodiode array detector.
abstract: This paper reports the validation of a quantitative high performance liquid chromatography-photodiode array (HPLC-PDA) method for the simultaneous analysis, in mouse plasma, of eperisone hydrochloride and paracetamol by protein precipitation using zinc sulphate-methanol-acetonitrile. The analytes were resolved on a Gemini C18 column (4.6 mm × 250 mm; 5 μm particle size) using a gradient elution mode with a run time of 15 min, comprising re-equilibration, at 60°C (± 1°C). The method was validated over the concentration range from 0.5 to 25 μg/mL for eperisone hydrochloride and paracetamol, in mouse plasma. Ciprofloxacin was used as Internal Standard. Results from assay validations show that the method is selective, sensitive and robust. The limit of quantification of the method was 0.5 μg/mL for eperisone hydrochloride and paracetamol, and matrix-matched standard curves showed a good linearity, up to 25 μg/mL with correlation coefficients (r(2))≥ 0.9891. In the entire analytical range the intra and inter-day precision (RSD%) values were ≤ 1.15% and ≤ 1.46% for eperisone hydrochloride, and ≤ 0.35% and ≤ 1.65% for paracetamol. For both analytes the intra and inter-day trueness (bias%) values ranged, respectively, from -5.33% to 4.00% and from -11.4% to -4.00%. The method was successfully tested in pharmacokinetic studies after oral administration in mouse. Furthermore, the application of this method results in a significant reduction in terms of animal number, dosage, and improvement in speed, rate of analysis, and quality of pharmacokinetic parameters related to serial blood sampling.
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy; Interuniversity Consortium of Structural and Systems Biology, Viale Medaglie d'oro 305, 00136 Roma, Italy. Electronic address: m.locatelli@unich.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Locatelli
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Cifelli
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Di Legge
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Immunology Division, Department of Experimental and Clinical Science, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Renato Carmine
authors.initials: RC
authors.lastname: Barbacane
authors.affiliation: University of Catanzaro "Magna Graecia", Department of Health Sciences, Viale "S. Venuta", 88100 Catanzaro, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Costa
authors.affiliation: University of Catanzaro "Magna Graecia", Department of Health Sciences, Viale "S. Venuta", 88100 Catanzaro, Italy; University of Catanzaro "Magna Græcia", Inter-regional Research Center for Food Safety & Health, Viale "S. Venuta", 88100 Catanzaro, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Fresta
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy; University of Catanzaro "Magna Græcia", Inter-regional Research Center for Food Safety & Health, Viale "S. Venuta", 88100 Catanzaro, Italy; Houston Methodist Research Institute, Department of Nanomedicine, Houston, TX 77030, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Celia
authors.affiliation: Unità Operativa di Farmacia Ospedaliera, Presidio Ospedaliero Soveria Mannelli, Viale R. Rubbettino, 88049 Soveria Mannelli (CZ), Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Capolupo
authors.affiliation: University "G. d'Annunzio" Chieti-Pescara, Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Di Marzio
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D011427:Propiophenones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D014018:Tissue Distribution; D054928:Validation Studies as Topic
chemical_list: D011427:Propiophenones; C030848:eperisone; D000082:Acetaminophen
keywords: Eperisone hydrochloride; HPLC-PDA; Method development; Paracetamol; Plasma; Sample preparation