title: Hybridization-sensitive fluorescent oligonucleotide probe conjugated with a bulky module for compartment-specific mRNA monitoring in a living cell.
abstract: Live-cell RNA imaging at specific intracellular locations is technically limited because of the diffusive nature of small oligonucleotide probes. The bulky fluorescent light-up probes that possess streptavidin or gold nanoparticles at the end of oligonucleotides were designed and synthesized. The bulky probes allowed nucleus- and cytoplasm-selective monitoring of endogenous mRNAs through nuclear and cytoplasmic microinjection, respectively. Simultaneous use of bulky and unbulky probes conjugated with different fluorescent dyes enabled dual color imaging of mRNAs present in nucleus and cytoplasm. Furthermore, we observed that the fluorescence near the cell edge in a living HeLa cell traveled over time in coordination with the dynamic formation and deformation of the pseudopodial protrusions after lipofection of the bulky probes.
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Gosuke
authors.initials: G
authors.lastname: Hayashi
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yanase
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Takeda
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakakibara
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sakamoto
authors.affiliation: ‡Institute for Integrated Cell-Material Science (iCeMS), Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Dan Ohtan
authors.initials: DO
authors.lastname: Wang
authors.affiliation: †Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.|§Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry* / Q000502:physiology; D003593:Cytoplasm / Q000737:chemistry* / Q000502:physiology; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D022022:Nuclear Pore / Q000737:chemistry* / Q000502:physiology; D015345:Oligonucleotide Probes / Q000032:analysis / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger