title: The timing of acid-induced increase in saliva secretion in transplanted submandibular glands.
abstract: The purpose of this study was to determine the timing of acid-induced increase in saliva secretion and to investigate the possibility of parasympathetic reinnervation of transplanted submandibular glands (SMGs). Citric acid stimulation-induced changes in secretion of transplanted SMGs were evaluated in 27 patients who underwent SMG transplantation for keratoconjunctivitis sicca (KCS); (99m)Tc scintigraphy and Schirmer tests were done at 1, 3, 6, and 9 months after transplantation. Acetylcholinesterase staining was conducted to confirm the presence of parasympathetic reinnervation in three SMGs at 6 and 9 months after transplantation. Schirmer tests showed significantly increased secretion of the transplanted SMGs after acid stimulation at 6 and 9 months, but not at 1 and 3 months. On (99m)Tc scintigraphy, no decline was detected on the dynamic time-activity curve after acid stimulation at 1 and 3 months, but a decline was detected in nine glands at 6 months and in 19 glands at 9 months. No decline was observed in the remaining eight glands at 9 months after transplantation. The histology findings were consistent with scintigraphy results. In conclusion, acid-induced increase in saliva secretion occurs at ≥6 months after SMG transplantation, and parasympathetic reinnervation of the transplanted SMG might occur.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Su
authors.affiliation: Department of Global Health, Peking University School of Public Health, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China. Electronic address: gyyu@263.net.
authors.forename: G Y
authors.initials: GY
authors.lastname: Yu
mesh_terms: D000293:Adolescent; D000328:Adult; D019343:Citric Acid / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007638:Keratoconjunctivitis Sicca / Q000000981:diagnostic imaging / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D012463:Saliva / Q000378:metabolism*; D012472:Salivation / Q000187:drug effects*; D013670:Sodium Pertechnetate Tc 99m; D013194:Staining and Labeling; D013363:Submandibular Gland / Q000378:metabolism* / Q000637:transplantation*; D013997:Time Factors
chemical_list: D019275:Radiopharmaceuticals; D019343:Citric Acid; D013670:Sodium Pertechnetate Tc 99m
keywords: Schirmer test; keratoconjunctivitis sicca; reinnervation; scintigraphy; submandibular gland transplantation