title: Blood vessel matrix seeded with cells: a better alternative for abdominal wall reconstruction-a long-term study.
abstract: OBJECTIVE
  The aim of this study was to present abdominal wall reconstruction using a porcine vascular graft seeded with MSC (mesenchymal stem cells) on rat model.
  
  
  METHODS
  Abdominal wall defect was prepared in 21 Wistar rats. Acellular porcine-vascular grafts taken from aorta and prepared with Triton X were used. 14 aortic grafts were implanted in place, of which 7 grafts were seeded with rat MSC cells (Group I), and 7 were acellular grafts (Group II). As a control, 7 standard polypropylene meshes were used for defect augmentation (Group III). The assessment method was performed by HE and CD31 staining after 6 months. The mechanical properties have been investigated by Zwick&Roell Z0.5.
  
  
  RESULTS
  The strongest angiogenesis and lowest inflammatory response were observed in Group I. Average capillaries density was 2.75, 0.75, and 1.53 and inflammatory effect was 0.29, 1.39, and 2.72 for Groups I, II, and III, respectively. The means of mechanical properties were 12.74 ± 1.48, 7.27 ± 1.56, and 14.4 ± 3.7 N/cm in Groups I and II and control, respectively.
  
  
  CONCLUSIONS
  Cell-seeded grafts have better mechanical properties than acellular grafts but worse than polypropylene mesh. Cells improved mechanical and physiological properties of decellularized natural scaffolds.
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland.
authors.forename: Maciej
authors.identifier: 0000-0001-8424-7198
authors.initials: M
authors.lastname: Nowacki
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Jundziłł
authors.affiliation: Department of General, Transplant and Liver Surgery, Warsaw Medical University, Ulica Banacha 1A, 02-097 Warsaw, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Nazarewski
authors.affiliation: Department of Orthopaedics and Traumatology of Musculoskeletal System, 1st Faculty of Medicine, Medical University of Warsaw, Ulica Lindleya 4, 02-005 Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kotela
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kloskowski
authors.affiliation: Department of Chemistry and Photochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Ulica Gagarina 7, 87-100 Torun, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Skopińska-Wisniewska
authors.affiliation: Department of Clinical Pathomorphology, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Skłodowskiej Curie 9, 85-094 Bydgoszcz, Poland.
authors.forename: Magdalena
authors.identifier: 0000-0003-3748-1058
authors.initials: M
authors.lastname: Bodnar
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Łukasiewicz
authors.affiliation: Department of General, Vascular, and Transplant Surgery, Warsaw Medical University, Ulica Banacha 1A, 02-097 Warsaw, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Nazarewski
authors.affiliation: Department of Orthopedic Surgery and Traumatology, Central Research Hospital of Ministry of Interior, Ulica Wołoska 137, 02-507 Warsaw, Poland ; Institute of Physiotherapy, The Jan Kochanowski University of Humanities and Sciences, Al. IX Wieków Kielc 19, 25-317 Kielce, Poland.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Kotela
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Chair and Department of Descriptive and Topographic Anatomy, Medical University of Silesia, Ulica Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Marek
authors.identifier: 0000-0001-7950-679X
authors.initials: M
authors.lastname: Kucharzewski
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pokrywczyńska
authors.affiliation: Department of Clinical Pathomorphology, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Skłodowskiej Curie 9, 85-094 Bydgoszcz, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszałek
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University in Torun, Ulica Karlowicza 24, 85-092 Bydgoszcz, Poland ; Department of Urology, Nicolaus Copernicus Hospital in Torun, Ulica Batorego 17/19, 87-100 Torun, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D034861:Abdominal Wall / Q000254:growth & development / Q000473:pathology / Q000601:surgery*; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000637:transplantation; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000254:growth & development / Q000473:pathology / Q000637:transplantation*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D019651:Plastic Surgery Procedures; D013552:Swine