title: Light influence in the nutritional composition of Brassica oleracea sprouts.
abstract: Brassica sprouts are considered a healthy food product, whose nutritional quality can be influenced by several factors. The aim of this work was to monitor the nutritional composition changes promoted by different sprouting conditions of four varieties of Brassica oleracea (red cabbage, broccoli, Galega kale and Penca cabbage). Sprouts were grown under light/darkness cycles and complete darkness. Standard AOAC methods were applied for nutritional value evaluation, while chromatographic methods with UV-VIS and FID detection were used to determine the free amino acids and fatty acids, respectively. Mineral content was analyzed by atomic absorption spectrometry. Sprouts composition revealed them as an excellent source of protein and dietary fiber. Selenium content was one of the most distinctive feature of sprouts, being the sprouting conditions determinant for the free amino acid and fatty acids profile. The use of complete darkness was beneficial to the overall nutritional quality of the brassica sprouts studied.
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; ESA, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: A P
authors.initials: AP
authors.lastname: Vale
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Santos
authors.affiliation: ESA, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: N V
authors.initials: NV
authors.lastname: Brito
authors.affiliation: ESA, Instituto Politécnico de Viana do Castelo, Refóios do Lima, 4990-706 Ponte de Lima, Portugal.
authors.forename: V
authors.initials: V
authors.lastname: Peixoto
authors.affiliation: Departamento de Agronomia, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal.
authors.forename: Rosa
authors.initials: R
authors.lastname: Carvalho
authors.affiliation: CITAB/UTAD-Centre for the Research and Technology for Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Rosa
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address: beatoliv@ff.up.pt.
authors.forename: M Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
mesh_terms: D001937:Brassica / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D003624:Darkness; D004043:Dietary Fiber; D058870:Food, Organic / Q000032:analysis / Q000528:radiation effects; D008027:Light; D009753:Nutritive Value / Q000528:radiation effects*; D018520:Plant Shoots / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects; D036226:Seedlings; D012643:Selenium / Q000032:analysis
chemical_list: D004043:Dietary Fiber; D012643:Selenium
keywords: Amino acids; Brassica sprouts; Fatty acids profile; Minerals; Nutritional evaluation; Sprouting conditions