title: Influences of domoic Acid exposure on cardiac development and the expression of cardiovascular relative genes in zebrafish (Daniorerio) embryos.
abstract: Domoic acid (DA) is a highly toxic phycotoxin that is generated from marine diatoms Pseudonitzschia spp. It has been found that bivalves or cephalopods can accumulate DA to a high level through their feeding activities and cause illness or death in consumers. Zebrafish have been used as a model to investigate and characterize the developmental toxicity of DA. However, there is no report about the relationship between DA and cardiac development in zebrafish. Here, zebrafish embryos were exposed to DA with at the dose of 1, 10, 100, and 1000 ng/L. High mortality and some developmental toxicity including pericardial and yolk sac edema, dorsal curvature, and cardiac defects were observed in the DA-treated larvae. We found that DA exposure not only disrupted normal cardiac development but also altered the expression of some cardiac development correlated genes and calcium ion channels, such as Anf, Bnp, Atp2a2a, Atp2a2b, Ncx1h, Ryr2b, and Tbx5.
authors.affiliation: School of Life Sciences and The State Key Laboratory of Marine Environmental Science, Xiamen University, South Xiangan Road, Xiamen, 361102, People's Republic of China. gaoyh@xmu.edu.cn.|Engineering Research Center of Marine Biological Resource Comprehensive Utilization, The Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), 184 Daxue Road, Xiamen, 361005, China. gaoyh@xmu.edu.cn.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Hong
authors.affiliation: School of Life Sciences and The State Key Laboratory of Marine Environmental Science, Xiamen University, South Xiangan Road, Xiamen, 361102, People's Republic of China.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Life Sciences and The State Key Laboratory of Marine Environmental Science, Xiamen University, South Xiangan Road, Xiamen, 361102, People's Republic of China.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Engineering Research Center of Marine Biological Resource Comprehensive Utilization, The Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), 184 Daxue Road, Xiamen, 361005, China.
authors.forename: Ruilin
authors.initials: R
authors.lastname: Zhu
authors.affiliation: School of Life Sciences and The State Key Laboratory of Marine Environmental Science, Xiamen University, South Xiangan Road, Xiamen, 361102, People's Republic of China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D066126:Cardiotoxicity; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D008387:Marine Toxins / Q000633:toxicity*; D038081:Organogenesis / Q000187:drug effects* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015027:Zebrafish
chemical_list: D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid
keywords: Cardiac Toxicity; Domoic Acid; Embryonic Development; Zebrafish