title: MiR-124 promotes bone marrow mesenchymal stem cells differentiation into neurogenic cells for accelerating recovery in the spinal cord injury.
abstract: In this research, mouse BMMSCs were isolated from bone marrow, induced to differentiate into neurogenic cells in vitro, and transplanted into the injured spinal cord after over-expression of miR-124. The results showed that the BMMSCs could induce the differentiation to neurogenic cells under the special condition medium, but when the miR-124 was over-expressed, the differentiation efficiency of neurogenic cells from BMMSCs could be promoted. This reason was demonstrated that polypyrimidine tract-binding protein 1 (PTBP1) showed a repressor for polypyrimidine tract-binding protein 2 (PTBP2) during neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. miR-124 promoted neurogenic cells from BMMSCs were successful colonized into injured spinal cord for participation in tissue-repair. In conclusion, our research shows that the miR-124 promoted the differentiation of neuronal cells from BMMSCs, and this mechanism was miR-124 reduced the expression of PTBP1, increased the expression of PTBP2.
authors.affiliation: Department of Orthopedics, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110016, Liaoning Province, China.
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110016, Liaoning Province, China.
authors.forename: Liang-Bi
authors.initials: LB
authors.lastname: Xiang
authors.affiliation: Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110016, Liaoning Province, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China. Electronic address: jiantingchenprof@163.com.
authors.forename: Jian-Ting
authors.initials: JT
authors.lastname: Chen
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D013119:Spinal Cord Injuries / Q000628:therapy*
chemical_list: D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse
keywords: Bone marrow; MSCs; Neurogenic cells; microRNA