title: Single-molecule arrays for ultrasensitive detection of host immune response to dengue virus infection.
abstract: Dengue virus serotype 2-positive plasma was applied to two indirect single-molecule arrays (Simoas) for the detection of anti-dengue virus IgG and IgM. The Simoas were 1,000 and 10,000 times more sensitive than enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM, respectively. Using Simoas, serology may be used for the detection of dengue virus infection in the acute phase.
authors.affiliation: Department of Chemistry, School of Arts and Sciences, Tufts University, Medford, Massachusetts, USA.
authors.forename: Shonda T
authors.initials: ST
authors.lastname: Gaylord
authors.affiliation: Department of Chemistry, School of Arts and Sciences, Tufts University, Medford, Massachusetts, USA.
authors.forename: Sama
authors.initials: S
authors.lastname: Abdul-Aziz
authors.affiliation: Department of Chemistry, School of Arts and Sciences, Tufts University, Medford, Massachusetts, USA david.walt@tufts.edu.
authors.forename: David R
authors.initials: DR
authors.lastname: Walt
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M