title: Production of the allergenic protein Alt a 1 by Alternaria isolates from working environments.
abstract: The aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibit Alt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%-16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata, A. lini, A. limoniasperae A. nobilis and A. tenuissima. Environmental strains produced Alt a 1 at higher concentrations (1.103-6.528 ng/mL) than a ATCC strain (0.551-0.975 ng/mL). It has been shown that the homogenization of the mycelium and the use of ultrafiltration allow a considerable increase of Alt a 1 concentration. Variations in the production of Alt a 1 protein, depend on the strain and extraction methods. These studies revealed no impact of the technical material from the workplaces on the production of Alt a 1 protein.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland. justyna-skora@wp.pl.
authors.forename: Justyna
authors.initials: J
authors.lastname: Skóra
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland. anna.otlewska@p.lodz.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Otlewska
authors.affiliation: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland. beata.gutarowska@p.lodz.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Gutarowska
authors.affiliation: Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland. joanna.leszczynska@p.lodz.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Leszczyńska
authors.affiliation: Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland. iwona.majak@gmail.com.
authors.forename: Iwona
authors.initials: I
authors.lastname: Majak
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska Str, 60-479 Poznań, Poland. lste@igr.poznan.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Stępień
mesh_terms: D016902:Air Pollution, Indoor; D000485:Allergens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000528:Alternaria / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D016273:Occupational Exposure
chemical_list: C096258:ALTA1 protein, Alternaria alternata; D000485:Allergens; D005656:Fungal Proteins