title: Plasma rich in growth factors (PRGF) eye drops stimulates scarless regeneration compared to autologous serum in the ocular surface stromal fibroblasts.
abstract: Autologous serum (AS) eye drops was the first blood-derived product used for the treatment of corneal pathologies but nowadays PRGF arises as a novel interesting alternative to this type of diseases. The purpose of this study was to evaluate and compare the biological outcomes of autologous serum eye drops or Plasma rich in growth factors (PRGF) eye drops on corneal stromal keratocytes (HK) and conjunctival fibroblasts (HConF). To address this, blood from healthy donors was collected and processed to obtain autologous serum (AS) eye drops and plasma rich in growth factors (PRGF) eye drops. Blood-derivates were aliquoted and stored at -80°C until use. PDGF-AB, VEGF, EGF, FGFb and TGF-β1 were quantified. The potential of PRGF and AS in promoting wound healing was evaluated by means of proliferation and migration assays in HK and HConF. Fibroblast cells were induced to myofibroblast differentiation after treatment with 2.5ng/mL of TGF-β1. The capability of PRGF and AS to prevent and inhibit TGF-β1-induced differentiation was evaluated. Results showed significant higher levels of all growth factors analyzed in PRGF eye drops compared to AS. Moreover, PRGF eye drops enhanced significantly the biological outcomes of both HK and HConF, and reduced TGF-β1-induced myofibroblast differentiation in contrast to autologous serum eye drops (AS). In summary, these results suggest that PRGF exerts enhanced biological outcomes than AS. PRGF may improve the treatment of ocular surface wound healing minimizing the scar formation compared to AS. Results obtained herein suggest that PRGF protects and reverses the myofibroblast phenotype while promotes cell proliferation and migration.
authors.affiliation: Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Anitua
authors.affiliation: Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: M
authors.initials: M
authors.lastname: de la Fuente
authors.affiliation: Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Muruzabal
authors.affiliation: Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernández-Vega, Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Riestra
authors.affiliation: Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernández-Vega, Oviedo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Merayo-Lloves
authors.affiliation: Biotechnology Institute (BTI), Vitoria, Spain. Electronic address: gorka.orive@ehu.es.
authors.forename: G
authors.initials: G
authors.lastname: Orive
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000517:prevention & control*; D003228:Conjunctiva / Q000166:cytology*; D060527:Corneal Keratocytes / Q000187:drug effects*; D003319:Corneal Stroma / Q000166:cytology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D053657:Platelet-Rich Plasma; D012038:Regeneration / Q000187:drug effects; D044967:Serum / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009883:Ophthalmic Solutions
keywords: Autologous serum; Cornea; Myofibroblast; PRGF; PRP; Plasma rich in growth factors; Platelet-rich plasma; Wound healing