title: Pre-exposure of neuroblastoma cell line to pulsed electromagnetic field prevents H2 O2 -induced ROS production by increasing MnSOD activity.
abstract: Electromagnetic fields (EMFs) have been linked to increased risk of cancers and neurodegenerative diseases; however, EMFs can also elicit positive effects on biological systems, and redox status seems crucially involved in EMF biological effects. This study aimed to assess whether a short and repeated pulsed EMF (PEMF) could trigger adaptive responses against an oxidative insult in a neuronal cellular model. We found that a 40 min overall (four times a week, 10 min each) pre-exposure to PEMF did not affect major physiological parameters and led to a significant increase of Mn-dependent superoxide dismutase activity in the human neuroblastoma SH-SY5Y cell line. In addition, we found PEMF-pre-exposed cells exhibited decreased reactive oxygen species production following a 30 min H2 O2 challenge, with respect to non pre-exposed cells. Our findings might provide new insights on the role played by short and repeated PEMF stimulations in the enhancement of cellular defenses against oxidative insults. Although studies in normal neuronal cells would be useful to further confirm our hypothesis, we suggest that specific PEMF treatments may have potential biological repercussions in diseases where oxidative stress is implicated.
authors.affiliation: Department of Drug Sciences, Section of Pharmacology, University of Pavia, Pavia, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Osera
authors.forename: Marialaura
authors.initials: M
authors.lastname: Amadio
authors.forename: Stefano
authors.initials: S
authors.lastname: Falone
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fassina
authors.forename: Giovanni
authors.initials: G
authors.lastname: Magenes
authors.forename: Fernanda
authors.initials: F
authors.lastname: Amicarelli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ricevuti
authors.forename: Stefano
authors.initials: S
authors.lastname: Govoni
authors.forename: Alessia
authors.initials: A
authors.lastname: Pascale
mesh_terms: D002452:Cell Count; D045744:Cell Line, Tumor; D000067780:ELAV-Like Protein 1 / Q000378:metabolism; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009447:Neuroblastoma / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D051571:Protein Kinase C-alpha / Q000378:metabolism; D000069079:Radiation Exposure; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000067780:ELAV-Like Protein 1; D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D051571:Protein Kinase C-alpha
keywords: SH-SY5Y; electromagnetism; oxidative stimulus; reactive oxygen species; superoxide dismutase