title: Muscarinic cholinoceptor-mediated activation of JNK negatively regulates intestinal secretion in mice.
abstract: Regulation of intestinal secretion is important for body fluid homeostasis. We investigated the role of three MAP kinases (MAPKs) as negative regulators in muscarinic cholinoceptor (mAChR)-mediated intestinal secretion in mice. Electrophysiological analyses revealed that mAChR stimulation enhanced intestinal chloride secretion, which was further augmented by the inhibition of JNK but not by that of ERK or p38 with specific inhibitors SP600125, U0126 or SB203580, respectively. Immunoblot analyses in colonic mucosa showed that mAChR stimulation increased MAPKs phosphorylation that was suppressed by the specific inhibitor for each MAPK. This suggests that JNK is a major negative regulator in mAChR-induced intestinal secretion.
authors.affiliation: Division of Cellular Signal Transduction, Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan; Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Md Rafiqul Islam
authors.initials: MR
authors.lastname: Khan
authors.affiliation: Division of Cellular Signal Transduction, Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Md Tariqul
authors.initials: MT
authors.lastname: Islam
authors.affiliation: Division of Cellular Signal Transduction, Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yazawa
authors.affiliation: Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan; Division of Health and Nutrition, Sendai Shirayuri Women's College, Sendai, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Division of Cellular Signal Transduction, Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan; Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Uwada
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Abu Syed Md
authors.initials: AS
authors.lastname: Anisuzzaman
authors.affiliation: Division of Cellular Signal Transduction, Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan. Electronic address: takotago@asahikawa-med.ac.jp.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002070:Butadienes / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007419:Intestinal Secretions / Q000187:drug effects*; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000873:Anthracenes; D002070:Butadienes; D007093:Imidazoles; D018721:Muscarinic Agonists; D009570:Nitriles; D011725:Pyridines; C113580:U 0126; C432165:pyrazolanthrone; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580
keywords: Intestinal secretion; MAP kinase; mAChR