title: Lipidomic profiling of di- and tri-acylglycerol species in weight-controlled mice.
abstract: Weight control by dietary calorie restriction (DCR) or exercise has been shown to prevent cancer in various models. However, the mechanisms as to how weight control is beneficial are not well understood. While previous reports have investigated the effects of weight control on total lipid levels or lipid composition within cellular membranes, there has been little work surrounding changes to individual lipids following weight control interventions. In this study, using a model of skin carcinogenesis centered on the tumor promotion stage, CD-1 mice were randomly assigned into 4 groups: ad libitum and sedentary (control), ad libitum with exercise (AL+Exe), exercise with pair feeding of a diet isocaloric with control (PF+Exe), and sedentary with 20% DCR compared to control. After ten weeks, body weight and body fat percentages significantly decreased in the PF+Exe and DCR groups but not AL+Exe when compared with sedentary controls. Murine skin and plasma samples were obtained for analysis. Lipidomics using electrospray ionization MS/MS was employed to profile triacylglycerol (TG) and diacylglycerol (DG) species. Both plasma and tissue TG species containing fatty acid chains with length 18:1 were significantly decreased following DCR when compared to sedentary control animals. In regards to DG, the most significant changes occurred in the plasma. DG species containing fatty acids with lengths 16:1 or 18:1 were significantly decreased in PF+Exe and DCR groups when compared to sedentary controls. Due to the significant role of TG in energy storage and DG in cellular signaling, our findings of the effects of weight control on individual TG and DG species in plasma and skin tissue following exposure to a tumor promoter, may provide insight into the mechanism of weight control on cancer prevention.
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Brenee S
authors.initials: BS
authors.lastname: King
authors.affiliation: Institute of Animal Husbandry & Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Joseph
authors.initials: J
authors.lastname: Standard
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Su
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, United States of America.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D031204:Caloric Restriction; D004075:Diglycerides / Q000378:metabolism*; D051379:Mice; D010805:Physical Conditioning, Animal / Q000502:physiology; D012867:Skin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D014280:Triglycerides