title: Single-nucleotide polymorphisms of microRNA processing machinery genes and risk of colorectal cancer.
abstract: OBJECTIVE
  MicroRNA (miRNA)-related single-nucleotide polymorphisms (miR-SNPs) in miRNA processing machinery genes can affect cancer risk, treatment efficacy, and patient prognosis. We genotyped 6 miR-SNPs of miRNA processing machinery genes including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348) in a case-control study to evaluate their impact on colorectal cancer (CRC) risk.
  
  
  METHODS
  miR-SNPs were genotyped using the polymerase chain reaction- ligase detection reaction. The χ (2) test was used to analyze dichotomous values, such as the presence or absence of any individual SNP in CRC patients and healthy controls.
  
  
  RESULTS
  Two of these SNPs were identified for their association with cancer risk in the Dicer and GEMIN3 genes. The AA allele of rs3742330 located in the Dicer gene exhibited a significantly increased risk of CRC (odds ratio, 2.11; 95% confidence interval: 1.33-3.34; P=0.001); the TT allele of rs197412 located in GEMIN3 also exhibited a significantly increased risk of CRC (odds ratio, 1.68; 95% confidence interval: 1.07-2.65; P=0.024).
  
  
  CONCLUSIONS
  Our results suggest that the specific genetic variants in miRNA machinery genes may affect CRC susceptibility.
authors.affiliation: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Guo
keywords: CRC; Dicer; GEMIN3; miR-SNP