title: Identification and quantification of fumonisin A1, A2, and A3 in corn by high-resolution liquid chromatography-orbitrap mass spectrometry.
abstract: Three compounds, hypothesized as fumonisin A1 (FA1), fumonisin A2 (FA2), and fumonisin A3 (FA3), were detected in a corn sample contaminated with mycotoxins by high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS). One of them has been identified as FA1 synthesized by the acetylation of fumonisin B1 (FB1), and established a method for its quantification. Herein, we identified the two remaining compounds as FA2 and FA3, which were acetylated fumonisin B2 (FB2) and fumonisin B3 (FB3), respectively. Moreover, we examined a method for the simultaneous analysis of FA1, FA2, FA3, FB1, FB2, and FB3. The corn samples were prepared by extraction using a QuEChERS kit and purification using a multifunctional cartridge. The linearity, recovery, repeatability, limit of detection, and limit of quantification of the method were >0.99, 82.9%-104.6%, 3.7%-9.5%, 0.02-0.60 μg/kg, and 0.05-1.98 μg/kg, respectively. The simultaneous analysis of the six fumonisins revealed that FA1, FA2, and FA3 were present in all corn samples contaminated with FB1, FB2, and FB3. The results suggested that corn marketed for consumption can be considered as being contaminated with both the fumonisin B-series and with fumonisin A-series. This report presents the first identification and quantification of FA1, FA2, and FA3 in corn samples.
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. masayoshi.tamura@asahigroup-holdings.com.|Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. masayoshi.tamura@asahigroup-holdings.com.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. naoki.mochizuki@asahigroup-holdings.com.
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. yasushi.nagatomi@asahigroup-holdings.com.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nagatomi
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. koichi.harayama@asahigroup-holdings.com.
authors.forename: Koichi
authors.initials: K
authors.lastname: Harayama
authors.affiliation: Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. toriba@p.kanazawa-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.affiliation: Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, Japan. hayakawa@p.kanazawa-u.ac.jp.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D013058:Mass Spectrometry; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins; C000602130:fumonisin A3; C056934:fumonisin B2; C092632:fumonisin A2; C092631:fumonisin A1; C073063:fumonisin B3; C056933:fumonisin B1