title: High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions.
abstract: BACKGROUND
  Plutella xylostella (Linnaeus) has become the most destructive pest in cabbage throughout the world. Cry1Ia8 cabbage has been developed to reduce pest attacks. To gain a better understanding of the efficacy of Cry1Ia8 cabbage, a homozygous Cry1Ia8 cabbage line A14-5 was produced, and its resistance to P. xylostella, Pieris rapae (Linnaeus) and other lepidopteran pests was evaluated in the field in 2011, 2012 and 2013.
  
  
  RESULTS
  Under natural infestation conditions, the homozygous transgenic line was highly resistant against P. xylostella and P. rapae as compared with the untransformed control and susceptible to Mamestra brassicae (Linnaeus) and Spodoptera exigua (Hübner). The homozygous transgenic plants showed slight symptoms of damaged leaves by lepidopteran species, while the untransformed plants exhibited serious damage symptoms throughout the cabbage growing season.
  
  
  CONCLUSIONS
  Compared with the control, the homozygous transgenic cabbage line showed great potential for protecting cabbage from attack by P. xylostella and P. rapae in the field.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.|Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Dengxia
authors.initials: D
authors.lastname: Yi
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001937:Brassica / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D018556:Crops, Agricultural; D060467:Disease Resistance; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism; D005813:Genes, Synthetic / Q000235:genetics; D006720:Homozygote; D007915:Lepidoptera / Q000502:physiology*; D009036:Moths / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology* / Q000469:parasitology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D030821:Plants, Genetically Modified; D018411:Spodoptera / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins
keywords: Bacillus thuringiensis; Pieris rapae; Plutella xylostella; cry1Ia8 gene; resistance evaluation; transgenic cabbage