title: RHCE variant allele: RHCE*ce254G,733G.
abstract: A novel RHCE allele was identified in a 53-year-old African American female blood donor with an Rh phenotype of D+ C– E– c+ e+ and a negative antibody screen. The donor’s cells typed e+ with all antisera tested. By gel-based genotyping and cDNA analysis, the two RHCE alleles in this donor were characterized. One allele was found to be the known allele RHCE*01.20.01 (RHCE*ce733G) and the second was novel: RHCE*01.06.02 (RHCE*ce254G,733G).
authors.affiliation: MS, Lead Molecular Technologist.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Keller
authors.affiliation: MS, Supervisor, American Red Cross, National Molecular Laboratory.
authors.forename: Trina
authors.initials: T
authors.lastname: Horn
authors.affiliation: Bonfils Blood Center.
authors.forename: Colleen
authors.initials: C
authors.lastname: Chiappa
authors.affiliation: Bonfils Blood Center, Denver, CO.
authors.forename: Camilla
authors.initials: C
authors.lastname: Melland
authors.affiliation: Cambridge, MA.
authors.forename: Christine
authors.initials: C
authors.lastname: Vietz
authors.affiliation: Hemocentro-Unicamp, R. Carlos Chagas, Campinas-SP, Brazil.
authors.forename: Lilian
authors.initials: L
authors.lastname: Castilho
authors.affiliation: PhD (corresponding author), Director, American Red Cross, National Molecular Laboratory, 700 Spring Garden Street, Philadelphia, PA 19123.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Keller
mesh_terms: D000483:Alleles; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D060005:Genotyping Techniques; D006801:Humans; D008875:Middle Aged; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; C066468:RHCE protein, human; D012204:Rh-Hr Blood-Group System