title: [Effects of local induction of ipt-gene in roots on cytokinins content in leaf cells tobacco plants].
abstract: Identification of cytokinins in differentiated leaf cells has received little attention. We have carried out immunohistochemical localization of cytokinins in leaves of transgenic tobacco plants in which the level of increased due to induced in their roots the expression of ipt-gene controlling cytokinin synthesis. Immuno-labeling of cytokinins with the help of antibodies raised against zeatin riboside was characteristic of mesophyll cells. The label was localized in cytoplasm adjacent to cell walls and was absent in vacuoles. Immunohistochemical staining also revealed the presence of cytokinins in guard cells. Induction of cytokinin synthesis enhanced the immunohistochemical staining of both mesophyll cells and guard cells, which was accompanied by elevated stomatal conductance. The possibility of a direct effect of cytokinins on stomatal conductance and their indirect influence through photosynthesis in the mesophyll cells is discussed.
authors.forename: L B
authors.initials: LB
authors.lastname: Vysotskaia
authors.forename: G P
authors.initials: GP
authors.lastname: Akhiiarova
authors.forename: G V
authors.initials: GV
authors.lastname: Sharapova
authors.forename: M A
authors.initials: MA
authors.lastname: Dedova
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Veselov
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Zaĭtsev
authors.forename: G P
authors.initials: GP
authors.lastname: Kudoiarova
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000906:Antibodies / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D003583:Cytokinins / Q000032:analysis / Q000096:biosynthesis*; D004553:Electric Conductivity; D018506:Gene Expression Regulation, Plant; D007150:Immunohistochemistry; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D058503:Mesophyll Cells / Q000737:chemistry / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D003583:Cytokinins; D007541:Isopentenyladenosine; C009699:zeatin riboside; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase