title: Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol.
abstract: Purified recombinant sorbose dehydrogenase from Sinorhizobium sp. 97507 exhibited high reactivity for 1,5-anhydro-D-glucitol (1,5-AG) and L-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (k(cat)/Km) for L-sorbose and 1,5-AG is 1.8 × 10(2) and 1.5 × 10(2) s(-1)·M(-1), respectively.
authors.affiliation: a Ikeda Food Research Co., Ltd , Fukuyama , Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Araki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakatsuka
authors.forename: Fuminao
authors.initials: F
authors.lastname: Kobayashi
authors.forename: Emi
authors.initials: E
authors.lastname: Watanabe-Ishimaru
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Sanada
authors.forename: Takashi
authors.initials: T
authors.lastname: Tamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Inagaki
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020579:Sinorhizobium / Q000201:enzymology* / Q000235:genetics; D013013:Sorbose / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose; D002237:Carbohydrate Dehydrogenases; C104557:sorbose dehydrogenase; D013013:Sorbose
keywords: 1,5-anhydro-d-glucitol; Sinorhizobium sp.; sorbose dehydrogenase