title: Coupling a solid phase microextraction (SPME) probe with ambient MS for rapid enrichment and detection of phosphopeptides in biological samples.
abstract: In this study, we developed a probe-electrospray ionization method by coupling a SPME probe modified with nanosized TiO2 directly to nanoESI-MS for the phosphoproteome analysis, which demonstrated excellent selectivity and sensitivity for enrichment of phosphopeptides in complex biological samples.
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 10084, China. xrzhang@mail.tsinghua.edu.cn.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Gong
authors.forename: Xingyu
authors.initials: X
authors.lastname: Si
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Wei
authors.forename: Chengdui
authors.initials: C
authors.lastname: Yang
authors.forename: Sichun
authors.initials: S
authors.lastname: Zhang
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006801:Humans; D010748:Phosphopeptides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D010748:Phosphopeptides; C009495:titanium dioxide; D014025:Titanium