title: Umbilical cord blood for autologous transfusion in the early postnatal ontogeny: analysis of cell composition and viability during long-term culturing.
abstract: Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of storage [corrected] and inability of cells to colony formation after 2 weeks.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia, romanov@cryocenter.ru.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Romanov
authors.forename: E E
authors.initials: EE
authors.lastname: Balashova
authors.forename: O A
authors.initials: OA
authors.lastname: Bystrykh
authors.forename: K V
authors.initials: KV
authors.lastname: Titkov
authors.forename: T N
authors.initials: TN
authors.lastname: Dugina
authors.forename: N V
authors.initials: NV
authors.lastname: Kabaeva
authors.forename: T A
authors.initials: TA
authors.lastname: Fedorova
authors.forename: O V
authors.initials: OV
authors.lastname: Rogachevskii
authors.forename: D N
authors.initials: DN
authors.lastname: Degtyarev
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D001773:Blood Cells / Q000502:physiology*; D001800:Blood Specimen Collection / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology* / Q000637:transplantation*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn