title: Import of proteins into isolated yeast mitochondria.
abstract: Mitochondria are essential organelles of eukaryotic cells. The vast majority of mitochondrial proteins is encoded within the nuclear genome and translocated into various mitochondrial compartments after translation in the cytosol as preproteins. Even in rather primitive eukaryotes like yeasts, there are 700-1,000 different proteins that need to be recognized in the cytosol, directed to the protein translocases in the two mitochondrial membranes and sorted to their appropriate mitochondrial subcompartment. In vitro reconstituted import systems have proved to be important tools to study these processes in detail. Using isolated mitochondria and radioactively labeled precursor proteins, it was possible to identify several import machineries and pathways consisting of a large number of components during the last few decades.
authors.affiliation: Cell Biology, University of Kaiserslautern, Erwin-Schrodinger-Strasse 13, 67663, Kaiserslautern, Germany.
authors.forename: Valentina
authors.initials: V
authors.lastname: Peleh
authors.forename: Ajay
authors.initials: A
authors.lastname: Ramesh
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Herrmann
mesh_terms: D002458:Cell Fractionation; D002474:Cell-Free System; D005656:Fungal Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D021381:Protein Transport; D015003:Yeasts / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins