title: NME2 reduces proliferation, migration and invasion of gastric cancer cells to limit metastasis.
abstract: Gastric cancer is one of the most common malignancies and has a high rate of metastasis. We hypothesize that NME2 (Nucleoside Diphosphate Kinase 2), which has previously been considered as an anti-metastatic gene, plays a role in the invasiveness of gastric cancer cells. Using a tissue chip technology and immunohistochemistry, we demonstrated that NME2 expression was associated with levels of differentiation of gastric cancer cells and their metastasis into the lymph nodes. When the NME2 gene product was over-expressed by ;in vitro stable transfection, cells from BGC823 and MKN45 gastric cancer cell lines had reduced rates of proliferation, migration, and invasion through the collagen matrix, suggesting an inhibitory activity of NME2 in the propagation and invasion of gastric cancer. NME2 could, therefore, severe as a risk marker for gastric cancer invasiveness and a potential new target for gene therapy to enhance or induce NME2 expression.
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Department of Pathology, Xi'an Children's Hospital, Xi'an, China.
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Yang
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Lihan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
authors.forename: Dongcang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Puget Sound Blood Center, Seattle, Washington, United States of America; Division of Hematology, Department of Medicine, University of Washington, School of Medicine, Seattle, Washington, United States of America.
authors.forename: Jing-fei
authors.initials: JF
authors.lastname: Dong
authors.affiliation: Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, Lanzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000502:physiology*; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; C517940:NME2 protein, human