title: Heterologous replicase driven 3' end repair of Cucumber mosaic virus satellite RNA.
abstract: To investigate the extent of the 3' end repair in a satellite RNA of Cucumber mosaic virus (CMV) strain Q (Q(sat)) by a heterologous Tomato aspermy virus (TAV), a set of biologically active agrotransformants corresponding to the three genomic RNAs of TAV was developed. Analysis of Nicotiana benthamiana plants agroinfiltrated with TAV and either wild type or each of the six 3' deletion mutants of Q(sat) revealed that (i) heterologous replicase failed to generate Q(sat) multimers, a hallmark feature of homologous replicase dependent replication of Qsat; (ii) manifestation of severe symptom phenotypes and progeny analysis suggested that heterologous replicase was competent to repair Q(sat) deletion mutants lacking up to 3'13 nucleotides (nt) but not beyond and (iii) comparative in silico analysis indicated that the 3' secondary structural features of the repaired Q(sat) progeny from heterologous vs homologous driven replicases are remarkably very similar. The significance of these observations is discussed.
authors.affiliation: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Sivanandam
authors.affiliation: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States.
authors.forename: Erika
authors.initials: E
authors.lastname: Varady
authors.affiliation: Department of Plant Pathology & Microbiology, University of California, Riverside, CA 92521-0122, United States. Electronic address: arao@ucr.edu.
authors.forename: A L N
authors.initials: AL
authors.lastname: Rao
mesh_terms: D019401:Cucumber Mosaic Virus Satellite / Q000235:genetics / Q000502:physiology*; D017799:Cucumovirus / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D010935:Plant Diseases / Q000821:virology; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D017384:Sequence Deletion; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D019401:Cucumber Mosaic Virus Satellite; D012324:RNA-Dependent RNA Polymerase
keywords: 3′ end repair; Cucumovirus; Multimers; Replication; Satellite RNA