title: Rufibacter immobilis sp. nov., isolated from a high-altitude saline lake.
abstract: Two pinkish-red, Gram-stain-negative, non-motile aerobic bacterial strains (MCC P1(T) and MCC P2), capable of growing at low temperatures (15 °C), were isolated from water of a saline lake located in the western Himalayas of India. The strains were capable of growth in the presence of 0-2.0% NaCl and at pH 6.5-9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed the closest similarity of 96.3% to the type strain of the only species of the genus Rufibacter , Rufibacter tibetensis CCTCC AB 208084(T). Strains MCC P1(T) and MCC P2 shared 99.0% 16S rRNA gene sequence similarity and 88.6% DNA-DNA relatedness. The major cellular fatty acids were iso-C15 : 0, C17 : 1ω6c, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and summed feature 4 (anteiso-C17 : 1 B/iso-C17 : 1 I). Predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The respiratory quinone was MK-7. The DNA G+C content of the strains was 52.6-52.8 mol%. Based on morphological, physiological, chemotaxonomical and molecular characteristics, strains MCC P1(T) and MCC P2 represent a novel species of the genus Rufibacter , for which the name Rufibacter immobilis sp. nov. is proposed. The type strain is MCC P1(T) ( =MCC 2268(T) =CCTCC AB 2013351(T)).
authors.affiliation: Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors.forename: Ashish V
authors.initials: AV
authors.lastname: Polkade
authors.affiliation: Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors.forename: V Venkata
authors.initials: VV
authors.lastname: Ramana
authors.affiliation: Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors.forename: Amaraja
authors.initials: A
authors.lastname: Joshi
authors.affiliation: Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors.forename: Larrisa
authors.initials: L
authors.lastname: Pardesi
authors.affiliation: Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000531:Altitude; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7