title: Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China.
abstract: Canine parvovirus (CPV) can cause acute hemorrhagic diarrhea and fatal myocarditis in young dogs. Currently, most studies have focused on the evolution of the VP2 gene, whereas the full-length genome of CPV has been rarely reported. In this study, the whole genomes of CPV-LZ1 and CPV-LZ2 strains prevalent in Northwest China were determined and analyzed in comparison with those of the reference CPVs. The genome sequences of both LZ strains consisted of 5053 nucleotides. CPV-LZ1 and CPV-LZ2 strains were designated as new CPV-2a and CPV-2b, respectively. Sequence alignment analysis results revealed that these two new strains underwent specific unique variations during the process of local adaption. The left non-translated regions of these strains formed a Y-shaped hairpin structure, whereas the right non-translated regions lacked the reiteration of DNA sequence. A phylogenetic tree constructed from 33 whole coding regions of CPVs showed a strong spatial clustering, and these two strains belonged to the Chinese strain cluster lineage. This study provides a method to obtain the full-length genome of CPV. The isolation and characterization of these viruses adds incrementally to the knowledge of the full-length genome of CPV. The results from this study also provide insight into the molecular epidemiology and genetic diversity of the CPV field isolates from Northwest China and can be useful in preventing and controlling CPV infection in this region.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology and National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, People's Republic of China.
authors.forename: Shi-Chong
authors.initials: SC
authors.lastname: Han
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Guo
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Sun
authors.forename: Long
authors.initials: L
authors.lastname: Shu
authors.forename: Yan-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: De-Hui
authors.initials: DH
authors.lastname: Sun
authors.forename: Sui-Zhong
authors.initials: SZ
authors.lastname: Cao
authors.forename: Guang-Neng
authors.initials: GN
authors.lastname: Peng
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D014644:Genetic Variation; D016678:Genome / Q000235:genetics; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics*; D010802:Phylogeny; D015995:Prevalence; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins