title: A label-free electrochemical strategy for highly sensitive methyltransferase activity assays.
abstract: A new electrochemical strategy for the simple and ultrasensitive evaluation of DNA methyltransferase (MTase) activity was developed based on electrocatalytic oxidation of ascorbic acid by graphene. In addition, the suitability of this sensing platform for MTase inhibitor screening was also demonstrated.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China. chenjinhua@hnu.edu.cn.
authors.forename: Jiawan
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Erhu
authors.initials: E
authors.lastname: Xiong
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D004789:Enzyme Activation / Q000187:drug effects; D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D015254:DNA Modification Methylases; D001374:Azacitidine