title: A colorimetric and smartphone readable method for uracil-DNA glycosylase detection based on the target-triggered formation of G-quadruplex.
abstract: A simple, visible and smartphone readable strategy for the sensitive detection of uracil-DNA glycosylase (UDG) activity has been developed. This is based on the UDG-catalyzed removal of uracil bases, which induces the formation of a G-quadruplex-cofactor complex. The guanine-rich DNA probe can form a special G-quadruplex structure with hemin to display peroxidase activity and catalyze the H2O2-mediated oxidation of ABTS(2-) to the colored ABTS˙(-), providing a visible signal for UDG detection. The proposed sensing platform exhibits a good linear response to UDG concentrations ranging from 0.008 to 0.2 U mL(-1), with a low detection limit of 0.008 U mL(-1). Based on the theory of chromatics, we can directly read out the color value using a smartphone app to reflect the content of UDG with high resolution, providing a new vision for the portable assay strategy. Furthermore, the utility of this method for screening potential UDG inhibitors has also been demonstrated.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. wli@hnu.edu.cn.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Nie
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D001483:Base Sequence; D003124:Colorimetry / Q000295:instrumentation*; D057075:Enzyme Assays / Q000295:instrumentation*; D054856:G-Quadruplexes; D006861:Hydrogen Peroxide / Q000378:metabolism; D000068997:Smartphone; D051981:Uracil-DNA Glycosidase / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D051981:Uracil-DNA Glycosidase