title: Quantification of interferon signaling in avian cells.
abstract: Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.
authors.affiliation: Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
authors.forename: Joeri
authors.initials: J
authors.lastname: Kint
authors.forename: Maria
authors.initials: M
authors.lastname: Forlenza
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D017934:Coronavirus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism*; D011784:Quail; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D030161:Avian Proteins; D007370:Interferon Type I; D012333:RNA, Messenger