title: Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS).
abstract: Volumetric absorptive microsampling (VAMS) is a simple intuitive technique for collecting and quantitative analysis of dried blood samples. It enables the collection of an accurate blood volume (approximately 10μL) regardless of blood hematocrit. A bioanalytical method for the determination of paracetamol in dried blood supported on VAMS samplers has been validated and used to support a toxicokinetic (TK) study in rat. The calculated TK parameters were comparable to those obtained from blood-water (1:1, v/v) samples. VAMS is demonstrated to be a robust method that simplifies both the blood sample collection and bioanalytical laboratory procedures and generates high quality quantitative data. However, problems were encountered with controlling the bleed rate during sample collection, resulting in the VAMS tips being flooded and highlighting the need for bleeding methods to be compatible with microsampling techniques to avoid wasting blood. Alternative sample collection procedures are discussed that minimize these issues.
authors.affiliation: Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Denniff
authors.affiliation: Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Parry
authors.affiliation: Laboratory Animal Science, GlaxoSmithKline Research & Development, Ware, UK.
authors.forename: Wesley
authors.initials: W
authors.lastname: Dopson
authors.affiliation: Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK. Electronic address: neil.spooner@gsk.com.
authors.forename: Neil
authors.initials: N
authors.lastname: Spooner
mesh_terms: D000082:Acetaminophen / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D005260:Female; D006400:Hematocrit; D051381:Rats; D017208:Rats, Wistar; D013048:Specimen Handling / Q000379:methods
chemical_list: D000082:Acetaminophen
keywords: Dried blood; Microsampling; Paracetamol; Validation; Volumetric absorptive microsampling