title: APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria.
abstract: We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL(-1)) and could reliably diagnose malaria in clinical blood samples.
authors.affiliation: Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R.. jatanner@hku.hk.
authors.forename: Roderick M
authors.initials: RM
authors.lastname: Dirkzwager
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Kinghorn
authors.forename: Jack S
authors.initials: JS
authors.lastname: Richards
authors.forename: Julian A
authors.initials: JA
authors.lastname: Tanner
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003124:Colorimetry; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D006801:Humans; D045022:Lactate Dehydrogenases / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D010963:Plasmodium falciparum / Q000201:enzymology*; D051381:Rats; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D045022:Lactate Dehydrogenases