title: Bimolecular fluorescence complementation (BiFC) technique in yeast Saccharomyces cerevisiae and mammalian cells.
abstract: Visualization of protein-protein interactions in vivo offers a powerful tool to resolve spatial and temporal aspects of cellular functions. The bimolecular fluorescence complementation (BiFC) makes use of nonfluorescent fragments of green fluorescent protein or its variants that are added as "tags" to target proteins under study. Only upon target protein interaction is a fluorescent protein complex assembled, and the site of interaction can be monitored by microscopy. In this chapter, we describe the method and tools for the use of BiFC in the yeast Saccharomyces cerevisiae and in mammalian cells.
authors.affiliation: Research Program in Cell and Molecular Biology, Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland.
authors.forename: Marion
authors.initials: M
authors.lastname: Weber-Boyvat
authors.forename: Shiqian
authors.initials: S
authors.lastname: Li
authors.forename: Kari-Pekka
authors.initials: KP
authors.lastname: Skarp
authors.forename: Vesa M
authors.initials: VM
authors.lastname: Olkkonen
authors.forename: Daoguang
authors.initials: D
authors.lastname: Yan
authors.forename: Jussi
authors.initials: J
authors.lastname: Jäntti
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D008164:Luminescent Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins