title: The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study.
abstract: BACKGROUND
  In this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitor cells.
  
  
  RESULTS
  After analyzing the samples by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), it could be shown that the number of expressed proteins differs depending on the developmental stages. Especially members of the functional classes, like proteins of biosynthesis, regulatory proteins and structural proteins, show the highest differential expression in the stages of development analyzed.
  
  
  CONCLUSIONS
  In this study, quantitative changes in the expression of proteins in the oB at different developmental stages (postnatal days (P) 7, 90 and 637) could be observed. Furthermore, the expression of many proteins was found at specific developmental stages. It was possible to identify these proteins which are involved in processes like support of cell migration and differentiation.
authors.affiliation: Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wille
authors.affiliation: Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Schümann
authors.affiliation: Proteome Center Rostock, Schillingallee 69, 18055 Rostock, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kreutzer
authors.affiliation: Proteome Center Rostock, Schillingallee 69, 18055 Rostock, Germany.
authors.forename: Michael O
authors.initials: MO
authors.lastname: Glocker
authors.affiliation: Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wree
authors.affiliation: Department of Pathology, Josef-Schneider-Str. 2, 97080 Würzburg, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Mutzbauer
authors.affiliation: Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schmitt
keywords: Brain; Development; Olfactory bulb; Proteomics; Rat