title: Characterisation of the metabolites of an antibacterial endophyte Botryodiplodia theobromae Pat. of Dracaena draco L. by LC-MS/MS.
abstract: Botryodiplodia theobromae Pat. belongs to the endophytic fungi that live within the tissues of medicinal plants and produce bioactive natural products. The endophyte was isolated from the leaves of Dracaena draco L. The LC-MS-based metabolite fingerprinting of the ethyl acetate extract of B. theobromae with antibacterial activity led to the identification of 13 metabolites pertaining to various classes: dipeptides (maculosin and L,L-cyclo(leucylprolyl), alkaloid (norharman), coumarin and isocoumarins (bergapten, meranzin and monocerin), sesquiterpene (dihydrocumambrin A), aldehyde (formyl indanone), fatty alcohol (halaminol A) and fatty acid amide (palmitoleamide, palmitamide, capsi-amide and oleamide). This study reports for the first time, the LC-MS and LC-MS/MS identification of 13 known bioactive metabolites from the antibacterial ethyl acetate extract of B.theobromae isolated from the leaves of D. draco L.
authors.affiliation: a Department of Drug Discovery and Development , Harrison School of Pharmacy, Auburn University , 4306 Walker Building, Auburn , AL 36849 , USA.|b Department of Pharmacognosy , Faculty of Pharmacy, Assiut University , Assiut , Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Zaher
authors.affiliation: c Mycology Center, Faculty of Science, Assiut University , Assiut , Egypt.
authors.forename: Ahmad M
authors.initials: AM
authors.lastname: Moharram
authors.affiliation: a Department of Drug Discovery and Development , Harrison School of Pharmacy, Auburn University , 4306 Walker Building, Auburn , AL 36849 , USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Davis
authors.affiliation: a Department of Drug Discovery and Development , Harrison School of Pharmacy, Auburn University , 4306 Walker Building, Auburn , AL 36849 , USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Panizzi
authors.affiliation: b Department of Pharmacognosy , Faculty of Pharmacy, Assiut University , Assiut , Egypt.
authors.forename: Makboul A
authors.initials: MA
authors.lastname: Makboul
authors.affiliation: a Department of Drug Discovery and Development , Harrison School of Pharmacy, Auburn University , 4306 Walker Building, Auburn , AL 36849 , USA.
authors.forename: Angela I
authors.initials: AI
authors.lastname: Calderón
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D001203:Ascomycota / Q000737:chemistry*; D002853:Chromatography, Liquid; D031409:Dracaena / Q000382:microbiology*; D060026:Endophytes / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018515:Plant Leaves / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents
keywords: Botryodiplodia theobromae; LC-MS/MS; antibacterial; metabolite fingerprinting