title: Effects of glucocorticoid-induced stress on absorption of glycylsarcosine in jejunum of broilers.
abstract: This study was conducted to investigate the effects of stress on the absorption of glycylsarcosine in the jejunum of broilers, as well as the underlying mechanisms. A glucocorticoid analogue, dexamethasone (DEX), was chosen to simulate the effects of endogenous glucocorticoids. Two hundred 21-day-old male Arbor Acre broilers were randomly allotted to 4 groups with 5 replicates in each group and 10 birds per replicate by a completely randomized assignment involving a single factorial arrangement of injection dose DEX. The broilers were injected with 1-mL DEX (0.1-, 0.5-, and 2.5-mg/kg BW) or saline subcutaneously in the abdomen 1×/d for 7 d. The broilers were weighed, killed, and sampled at 28 days old. The results showed that DEX dose-dependently reduced growth and endogenous corticosterone content in the plasma of the broilers (P<0.05). DEX decreased absorption of glycylsarcosine by jejunum brush border membrane vesicles and gut sacs (P<0.05). DEX treatment increased the crypt depth of the jejunum, decreased the villus height, absorption area, and ratio of villus height to crypt depth (P<0.05). Effect of dexamethasone on villus width was not obvious (P>0.05). It was concluded that DEX reduced the absorption of glycylsarcosine in the jejunum of broilers.
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: A J
authors.initials: AJ
authors.lastname: Zheng
authors.affiliation: Bureau of Agriculture and Animal Husbandry, Chengde 067000, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China liuguohua@caas.cn caihuiyi@caas.cn.
authors.forename: H Y
authors.initials: HY
authors.lastname: Cai
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China liuguohua@caas.cn caihuiyi@caas.cn.
authors.forename: G H
authors.initials: GH
authors.lastname: Liu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism* / Q000494:pharmacology; D004151:Dipeptides / Q000378:metabolism*; D005938:Glucocorticoids / Q000378:metabolism* / Q000494:pharmacology; D007583:Jejunum / Q000187:drug effects / Q000502:physiology*; D008297:Male; D011897:Random Allocation; D013312:Stress, Physiological
chemical_list: D004151:Dipeptides; D005938:Glucocorticoids; C004194:glycylsarcosine; D003907:Dexamethasone
keywords: absorption; broiler; glycylsarcosine; stress