title: Residue depletion of ampicillin in eggs.
abstract: A residue depletion study of ampicillin (AMP) was performed after oral dosing (60.0 mg/kg and 120.0 mg/kg body weight once a day for 5 days) to laying hens, through the use of reversed-phase high-performance liquid chromatography with fluorescence detection (RP-HPLC-FLD) to achieve detection of ampicillin residue in eggs. Limit of detection was 0.5 ng/g, and limit of quantitation was 1.2 ng/g for ampicillin. Extraction recoveries of ampicillin from samples fortified at 5.0-125.0 ng/g levels ranged from 77.5% to 84.6% in albumen, 77.9% to 87.5% in yolk, and 77.9% to 88.6% in whole egg, with coefficients of variation ≤ 9.3%. The maximum concentrations of ampicillin in albumen, yolk, and whole egg were detected at 1, 2, and 1 day after the last administration of ampicillin, respectively. Ampicillin was not detectable in albumen at day 9 of withdrawal time, at day 10 and 11 in yolk, and day 9 and 11 in whole egg at each of those 2 dose levels. The theoretical withdrawal time of AMP in whole egg was 6.730 and 7.296 days for 60 and 120 mg/kg oral dosage, respectively. This method also proved to be suitable as a rapid and reliable method for the determination of ampicillin in other poultry eggs.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: K-Z
authors.initials: KZ
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: H-S
authors.initials: HS
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: A-H
authors.initials: AH
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: G-X
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: G-J
authors.initials: GJ
authors.lastname: Dai
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.|Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000667:Ampicillin / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002645:Chickens / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005260:Female
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin