title: The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration.
abstract: Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
authors.affiliation: The Pirbright Institute, High Street, Compton, Newbury, Berkshire, RG20 7NN, UK, ruth.hennion@pirbright.ac.uk.
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Hennion
mesh_terms: D000818:Animals; D002645:Chickens; D017934:Coronavirus / Q000502:physiology*; D046509:Tissue Culture Techniques; D017186:Titrimetry; D014132:Trachea / Q000821:virology; D019562:Viral Load; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication