title: Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats.
abstract: BACKGROUND
  Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulin-receptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured.
  
  
  METHODS
  Adult Wistar male rats were divided into four groups (n=16 per group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet+melatonin; (iv) control diet+melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin.
  
  
  RESULTS
  After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of the MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated a body weight increase in high-fat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats.
  
  
  CONCLUSIONS
  The results indicate that an effect on gene expression of feeding behavior signals at the central nervous system (CNS) may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats.
authors.forename: María J
authors.initials: MJ
authors.lastname: Ríos-Lugo
authors.forename: Vanesa
authors.initials: V
authors.lastname: Jiménez-Ortega
authors.forename: Pilar
authors.initials: P
authors.lastname: Cano-Barquilla
authors.forename: Pilar Fernández
authors.initials: PF
authors.lastname: Mateos
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Spinedi
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Cardinali
authors.forename: Ana I
authors.initials: AI
authors.lastname: Esquifino
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat; D005247:Feeding Behavior / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D008550:Melatonin