title: Granulocyte-macrophage colony-stimulating factor-transfected bone marrow stromal cells for the treatment of ischemic stroke.
abstract: Adult, male, Sprague-Dawley rats were injected with granulocyte-macrophage colony-stimulating factor-transfected bone marrow stromal cells (GM-CSF-BMSCs) into the ischemic boundary zone at 24 hours after onset of middle cerebral artery occlusion. Results showed reduced infarct volume, decreased number of apoptotic cells, improved neurological functions, increased angiogenic factor expression, and increased vascular density in the ischemic boundary zone in rats that underwent GM-CSF-BMSCs transplantation compared with the BMSCs group. Experimental findings suggested that GM-CSF-BMSCs could serve as a potential therapeutic strategy for ischemic stroke and are superior to BMSCs alone.
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Xingjian
authors.initials: X
authors.lastname: Lin
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China ; Nanjing Medical University, Affiliated Nanjing First Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Liu
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Jingde
authors.initials: J
authors.lastname: Dong
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Di
authors.affiliation: Nanjing Medical University, Affiliated Nanjing Brain Hospital, Department of Neurology, Nanjing 210029, Jiangsu Province, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Shi
keywords: bone marrow stromal cells; gene transfection; granulocyte-macrophage colony-stimulating factor; ischemic stroke; neural regeneration; stem cells; transplantation