title: Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications.
abstract: Corynebacterium glutamicum (C. glutamicum) is a highly promising alternative prokaryotic host for recombinant protein expression, as it possesses several significant advantages over Escherichia coli (E. coli), the currently leading bacterial protein expression system. During the past decades, several experimental techniques and vector components for genetic manipulation of C. glutamicum have been developed and validated, including strong promoters for tightly regulating target gene expression, various types of plasmid vectors, protein secretion systems and methods of genetically modifying the host strain genome to improve protein production potential. This review critically discusses current progress in establishing C. glutamicum as a host for recombinant protein expression, and examines, in depth, some successful case studies of actual application of this expression system. The established "expression tool box" for developing novel constructs based on C. glutamicum as a host are also evaluated. Finally, the existing issues and solutions in process development with C. glutamicum as a host are specifically addressed.
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Liu
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Yankun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.affiliation: b Institute of Pharmacy & Biomedical Sciences, Strathclyde University , Glasgow , UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Jeffrey
authors.affiliation: b Institute of Pharmacy & Biomedical Sciences, Strathclyde University , Glasgow , UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Harvey
authors.affiliation: b Institute of Pharmacy & Biomedical Sciences, Strathclyde University , Glasgow , UK.
authors.forename: Brian
authors.initials: B
authors.lastname: McNeil
authors.affiliation: a National Engineering Laboratory of Cereal Fermentation Technology , School of Biotechnology, JiangNan University , Wuxi , China and.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: Bai
mesh_terms: D000818:Animals; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins
keywords: Corynebacterium glutamicum; genetic alteration of strain; heterologous genes; plasmids; promoters; protein secretion pathways