title: Dietary trans-10, cis-12-conjugated linoleic acid alters fatty acid metabolism and microbiota composition in mice.
abstract: The main aim of the present study was to investigate the effects of dietary trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) on intestinal microbiota composition and SCFA production. C57BL/6 mice (n 8 per group) were fed a standard diet either supplemented with t10c12-CLA (0·5 %, w/w) (intervention) or with no supplementation (control), daily for 8 weeks. Metabolic markers (serum glucose, leptin, insulin and TAG, and liver TAG) were assessed by ELISA commercial kits, tissue long-chain fatty acids and caecal SCFA by GC, and microbial composition by 16S rRNA pyrosequencing. Dietary t10c12-CLA significantly decreased visceral fat mass (P< 0·001), but did not affect body weight (intervention), when compared with no supplementation (control). Additionally, lipid mass and composition were affected by t10c12-CLA intake. Caecal acetate, propionate and isobutyrate concentrations were higher (P< 0·05) in the t10c12-CLA-supplemented group than in the control group. The analysis of the microbiota composition following 8 weeks of t10c12-CLA supplementation revealed lower proportions of Firmicutes (P= 0·003) and higher proportions of Bacteroidetes (P= 0·027) compared with no supplementation. Furthermore, t10c12-CLA supplementation for 8 weeks significantly altered the gut microbiota composition, harbouring higher proportions of Bacteroidetes, including Porphyromonadaceae bacteria previously linked with negative effects on lipid metabolism and induction of hepatic steatosis. These results indicate that the mechanism of dietary t10c12-CLA on lipid metabolism in mice may be, at least, partially mediated by alterations in gut microbiota composition and functionality.
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Marques
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy,Cork,Ireland.
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Fergus
authors.initials: F
authors.lastname: Shanahan
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Eamonn M
authors.initials: EM
authors.lastname: Quigley
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Cotter
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: John F
authors.initials: JF
authors.lastname: Cryan
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Dinan
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork,Cork,Ireland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
mesh_terms: D050154:Adiposity; D000818:Animals; D019440:Anti-Obesity Agents / Q000009:adverse effects*; D041963:Bacteroidetes / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000378:metabolism; D002432:Cecum; D019587:Dietary Supplements / Q000009:adverse effects*; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000378:metabolism*; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000382:microbiology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D050152:Intra-Abdominal Fat / Q000473:pathology; D044243:Linoleic Acids, Conjugated / Q000009:adverse effects*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D064307:Microbiota; D058889:Molecular Typing; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D009929:Organ Size
chemical_list: D019440:Anti-Obesity Agents; D015415:Biomarkers; D005232:Fatty Acids, Volatile; D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid
keywords: Lipid metabolism