title: Diabetes mellitus affects activity of calcium/calmodulin-dependent protein kinase II alpha in rat trigeminal ganglia.
abstract: The activity of calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα) may play a critical role in the modulation of nociceptor activity and plasticity of primary sensory trigeminal neurons. The aim of this study was to investigate the immunoreactivity of phosphorylated CaMKIIα (pCaMKIIα) in subpopulations of trigeminal ganglion (TG) neurons in rat models of early diabetes type 1 (dm1) and 2 (dm2). DM1 model was induced with intraperitoneally (i.p.) injected streptozotocin (STZ) (55mg/kg). DM2 rats were fed with the high fat diet (HFD) for 2 weeks and then received 35mg/kg of STZ i.p. Two weeks and 2 months after the STZ-diabetes induction, rats were sacrificed and immunohistochemical analysis for detection of pCaMKIIα immunoreactivity and double immunofluorescence labelling with isolectin (IB4) was performed. Increased intensity of pCaMKIIα immunofluorescence, restricted to IB4-negative small-diameter neurons, was seen in TG neurons two months after STZ-DM1 induction. DM1 model, as well as the obesity (control dm2 groups) resulted in neuronal impaired growth while dm2 model led to neuron hypertrophy in TG. Observed changes may play a critical role in the modulation of nociceptor activity and plasticity of primary sensory trigeminal neurons. In future, innovative strategies for modulation of CaMKIIα activity in specific subpopulations of neurons could be a novel approach in therapy of diabetic trigeminal neuropathy.
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Milka
authors.initials: M
authors.lastname: Jerić
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Vuica
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Matija
authors.initials: M
authors.lastname: Borić
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Livia
authors.initials: L
authors.lastname: Puljak
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Antonia
authors.initials: A
authors.lastname: Jeličić Kadić
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Ivica
authors.initials: I
authors.lastname: Grković
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia. Electronic address: natalija.filipovic@mefst.hr.
authors.forename: Natalija
authors.initials: N
authors.lastname: Filipović
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012668:Trigeminal Ganglion / Q000201:enzymology*; D053675:Versicans
chemical_list: D001786:Blood Glucose; D006023:Glycoproteins; D037102:Lectins; C508324:Vcan protein, rat; D053675:Versicans; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: CaMKII; Isolectin; Neuropathy; Trigeminal ganglion