title: Identification of protein receptors for coronaviruses by mass spectrometry.
abstract: As obligate intracellular parasites, viruses need to cross the plasma membrane and deliver their genome inside the cell. This step is initiated by the recognition of receptors present on the host cell surface. Receptors can be major determinants of tropism, host range, and pathogenesis. Identifying virus receptors can give clues to these aspects and can lead to the design of intervention strategies. Interfering with receptor recognition is an attractive antiviral therapy, since it occurs before the viral genome has reached the relative safe haven within the cell. This chapter describes the use of an immunoprecipitation approach with Fc-tagged viral spike proteins followed by mass spectrometry to identify and characterize the receptor for the Middle East respiratory syndrome coronavirus. This technique can be adapted to identify other viral receptors.
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 2040, Rotterdam, CA, 3000, The Netherlands.
authors.forename: V Stalin
authors.initials: VS
authors.lastname: Raj
authors.forename: Mart M
authors.initials: MM
authors.lastname: Lamers
authors.forename: Saskia L
authors.initials: SL
authors.lastname: Smits
authors.forename: Jeroen A A
authors.initials: JA
authors.lastname: Demmers
authors.forename: Huihui
authors.initials: H
authors.lastname: Mou
authors.forename: Berend-Jan
authors.initials: BJ
authors.lastname: Bosch
authors.forename: Bart L
authors.initials: BL
authors.lastname: Haagmans
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017934:Coronavirus / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005434:Flow Cytometry; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D047468:Immunoprecipitation; D011991:Receptors, Virus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D064370:Spike Glycoprotein, Coronavirus / Q000096:biosynthesis / Q000235:genetics; D053719:Tandem Mass Spectrometry; D053586:Virus Internalization
chemical_list: D017931:DNA Primers; D007074:Immunoglobulin G; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D064370:Spike Glycoprotein, Coronavirus; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4