title: Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT.
abstract: Monocyte chemotactic protein‑1 (MCP‑1/CCL2) is an important immune factor, which may be important in cancer progression by promoting proliferation, invasion, metastasis and the tumor microenvironment. Previous studies have demonstrated that CCL2 affects the proliferation of osteosarcoma cells via the RANKL signaling pathway. However, the underlying mechanisms remain to be elucidated. To investigate the role of CCL2 in osteosarcoma cells, MTT, spheroid forming, wound healing and transwell assays were performed to examine the proliferation and invasion abilities of the osteosarcoma cells. It was revealed that the high-grade osteosarcoma cells exhibited increased expression levels of CCL2 compared with the low-grade osteosarcoma cells (P<0.001). Furthermore, knockdown of CCL2 decreased the proliferation and invasion abilities of the osteosarcoma cells (P<0.01). These results suggested that the expression of CCL2 is high in high-grade osteosarcoma cells and promotes the proliferation and invasion of osteosarcoma cells.
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Quanchi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Shan
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Zhuoying
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Zifei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D034741:RNA, Small Interfering; D051057:Proto-Oncogene Proteins c-akt