title: Regulation of Leishmania (L.) amazonensis protein expression by host T cell dependent responses: differential expression of oligopeptidase B, tryparedoxin peroxidase and HSP70 isoforms in amastigotes isolated from BALB/c and BALB/c nude mice.
abstract: Leishmaniasis is an important disease that affects 12 million people in 88 countries, with 2 million new cases every year. Leishmania amazonensis is an important agent in Brazil, leading to clinical forms varying from localized (LCL) to diffuse cutaneous leishmaniasis (DCL). One interesting issue rarely analyzed is how host immune response affects Leishmania phenotype and virulence. Aiming to study the effect of host immune system on Leishmania proteins we compared proteomes of amastigotes isolated from BALB/c and BALB/c nude mice. The athymic nude mice may resemble patients with diffuse cutaneous leishmaniasis, considered T-cell hyposensitive or anergic to Leishmania's antigens. This work is the first to compare modifications in amastigotes' proteomes driven by host immune response. Among the 44 differentially expressed spots, there were proteins related to oxidative/nitrosative stress and proteases. Some correspond to known Leishmania virulence factors such as OPB and tryparedoxin peroxidase. Specific isoforms of these two proteins were increased in parasites from nude mice, suggesting that T cells probably restrain their posttranslational modifications in BALB/c mice. On the other hand, an isoform of HSP70 was increased in amastigotes from BALB/c mice. We believe our study may allow identification of potential virulence factors and ways of regulating their expression.
authors.affiliation: Heart Institute (InCor), University of São Paulo School of Medicine, São Paulo, São Paulo, Brazil.
authors.forename: Priscila Camillo
authors.initials: PC
authors.lastname: Teixeira
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Leonardo Garcia
authors.initials: LG
authors.lastname: Velasquez
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Lepique
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Eloiza
authors.initials: E
authors.lastname: de Rezende
authors.affiliation: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
authors.forename: José Matheus Camargo
authors.initials: JM
authors.lastname: Bonatto
authors.affiliation: Oswaldo Cruz Institute-FioCruz, Rio de Janeiro, Brazil.
authors.forename: Marcello Andre
authors.initials: MA
authors.lastname: Barcinski
authors.affiliation: Heart Institute (InCor), University of São Paulo School of Medicine, São Paulo, São Paulo, Brazil.
authors.forename: Edecio
authors.initials: E
authors.lastname: Cunha-Neto
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Beatriz Simonsen
authors.initials: BS
authors.lastname: Stolf
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D001938:Brazil; D004195:Disease Models, Animal; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007894:Leishmania mexicana / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D016774:Leishmaniasis, Diffuse Cutaneous / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010544:Peroxidases / Q000096:biosynthesis*; D020033:Protein Isoforms / Q000096:biosynthesis; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D018840:HSP70 Heat-Shock Proteins; D020033:Protein Isoforms; D015800:Protozoan Proteins; D010544:Peroxidases; C110959:tryparedoxin peroxidase; D012697:Serine Endopeptidases; C090703:oligopeptidase B