title: Simple chemiluminescence determination of ketotifen using tris(1,10 phenanthroline)ruthenium(II)- Ce(IV) system.
abstract: A new method using chemiluminescence (CL) detection has been developed for the simple determination of ketotifen fumarate (KF). The method is based on the catalytic effect of KF in the CL reaction of tris(1,10 phenanthroline)ruthenium(II), Ru(phen)3 (2+), with Ce(IV) in sulfuric acid medium. The CL response was detected using a lab-made chemiluminometer. Effects of chemical variables were investigated and under optimum conditions, the CL intensity was proportional to the concentration of the drug over the range 0.34-34.00 µg mL(-1) KF. The limit of detection (S/N=3) was 0.09 µg mL(-1). Effects of common ingredients were investigated and the method was applied successfully for determining KF in pharmaceutical formulations and human plasma. The percent of relative standard deviation (n=11) at level of 3.4 µg mL(-1) of KF was 4.6% and the minimum sampling rate was 70 samples per hour. The possible CL mechanism is proposed based on the kinetic characteristic of the CL reaction, CL spectrum, UV-Vis and phosphorescence spectra.
authors.affiliation: Department of Science, Golestan University, Gorgan, I.R. Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mokhtari
authors.affiliation: Department of Science, Golestan University, Gorgan, I.R. Iran.
authors.forename: Mehrgan
authors.initials: M
authors.lastname: Ghazaeian
authors.affiliation: Department of Science, Golestan University, Gorgan, I.R. Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Maghsoudi
authors.affiliation: Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, I.R. Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Keyvanfard
authors.affiliation: Department of Physics, Isfahan University of Technology, Isfahan, I.R. Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Emami
mesh_terms: D002563:Cerium / Q000737:chemistry*; D000069599:Crop Production; D007665:Ketotifen / Q000032:analysis*; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D010618:Phenanthrolines / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009942:Organometallic Compounds; D010618:Phenanthrolines; C034282:tris-(1,10-phenanthroline)ruthenium; D002563:Cerium; D007665:Ketotifen
keywords: chemiluminescence; human plasma; ketotifen; pharmaceuticals