title: Comparative genomic analysis of seven Mycoplasma hyosynoviae strains.
abstract: Infection with Mycoplasma hyosynoviae can result in debilitating arthritis in pigs, particularly those aged 10 weeks or older. Strategies for controlling this pathogen are becoming increasingly important due to the rise in the number of cases of arthritis that have been attributed to infection in recent years. In order to begin to develop interventions to prevent arthritis caused by M. hyosynoviae, more information regarding the specific proteins and potential virulence factors that its genome encodes was needed. However, the genome of this emerging swine pathogen had not been sequenced previously. In this report, we present a comparative analysis of the genomes of seven strains of M. hyosynoviae isolated from different locations in North America during the years 2010 to 2013. We identified several putative virulence factors that may contribute to the ability of this pathogen to adhere to host cells. Additionally, we discovered several prophage genes present within the genomes of three strains that show significant similarity to MAV1, a phage isolated from the related species, M. arthritidis. We also identified CRISPR-Cas and type III restriction and modification systems present in two strains that may contribute to their ability to defend against phage infection.
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Bumgardner
authors.affiliation: Systems Biology and Bioinformatics Research Group, Pilot Plant, Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkhuntien, Bangkok, Thailand.
authors.forename: Weerayuth
authors.initials: W
authors.lastname: Kittichotirat
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington.
authors.forename: Roger E
authors.initials: RE
authors.lastname: Bumgarner
authors.affiliation: Newport Laboratories Inc., Worthington, Minnesota.
authors.forename: Paulraj K
authors.initials: PK
authors.lastname: Lawrence
keywords: 
  CRISPR
  ; 
  Mycoplasma hyosynoviae
  ; Adhesin; MAA1; OppA; antibiotic; genome; phage; resistance