title: Carbon dots (C-dots) from cow manure with impressive subcellular selectivity tuned by simple chemical modification.
abstract: Improved cellular selectivity for nucleoli staining was achieved by simple chemical modification of carbon dots (C-dots) synthesized from waste carbon sources such as cow manure (or from glucose). The C-dots were characterized and functionalized (amine-passivated) with ethylenediamine, affording amide bonds that resulted in bright green fluorescence. The new modified C-dots were successfully applied as selective live-cell fluorescence imaging probes with impressive subcellular selectivity and the ability to selectively stain nucleoli in breast cancer cell lineages (MCF-7). The C-dots were also tested in four other cellular models and showed the same cellular selection in live-cell imaging experiments.
authors.affiliation: LIMA-Laboratório de Inorgânica e Materiais, Campus Universitário Darcy Ribeiro, CEP 70904-970, P.O. Box 4478, Brasilia-DF (Brazil), Also, Graduate Program from DQF-UFPE.
authors.forename: Cintya
authors.initials: C
authors.lastname: D'Angelis do E S Barbosa
authors.forename: José R
authors.initials: JR
authors.lastname: Corrêa
authors.forename: Gisele A
authors.initials: GA
authors.lastname: Medeiros
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Barreto
authors.forename: Kelly G
authors.initials: KG
authors.lastname: Magalhães
authors.forename: Aline L
authors.initials: AL
authors.lastname: de Oliveira
authors.forename: John
authors.initials: J
authors.lastname: Spencer
authors.forename: Marcelo O
authors.initials: MO
authors.lastname: Rodrigues
authors.forename: Brenno A D
authors.initials: BA
authors.lastname: Neto
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D006801:Humans; D008372:Manure / Q000032:analysis*; D061848:Optical Imaging / Q000379:methods*; D045663:Quantum Dots
chemical_list: D008372:Manure; D002244:Carbon
keywords: carbon dots; cell imaging; fluorescence; functionalized c-dots; nucleoli