title: Cross-talk between signaling pathways: the link between plant secondary metabolite production and wounding stress response.
abstract: Plants subjected to wounding stress produce secondary metabolites. Several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. This wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxygen species (ROS), ethylene (ET) and jasmonic acid (JA). However, their specific role and interactions that modulate the wound-respond in plants is not fully understood. In the present study, a subtractive cDNA library was generated, to better understand the global response of plants to wounding stress. Carrot (Daucus carota) was used as a model system for this study. A total of 335 unique expressed sequence tags (ESTs) sequences were obtained. ESTs sequences with a putative identity showed involvement in stress-signaling pathways as well as on the primary and secondary metabolism. Inhibitors of ROS biosynthesis, ET action, and JA biosynthesis alone and in combination were applied to wounded-carrots in order to determine, based on relative gene expression data, the regulatory role of ET, JA, and ROS on the wound-response in plants. Our results demonstrate that ROS play a key role as signaling-molecules for the wound-induced activation of the primary and secondary metabolism whereas ET and JA are essential to modulate ROS levels.
authors.affiliation: Department of Horticultural Sciences, Texas A‌&‌M University, Vegetable ‌&‌ Fruit Improvement Center, College Station, TX 77843-2133, U.S.A.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Jacobo-Velázquez
authors.affiliation: Institute of Agricultural Research (INIA-CRI La Platina), Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: González-Agüero
authors.affiliation: Department of Horticultural Sciences, Texas A‌&‌M University, Vegetable ‌&‌ Fruit Improvement Center, College Station, TX 77843-2133, U.S.A.
authors.forename: Luis
authors.initials: L
authors.lastname: Cisneros-Zevallos
mesh_terms: D001481:Basal Metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018552:Daucus carota / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008954:Models, Biological; D054883:Oxylipins / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D064210:Secondary Metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014947:Wounds and Injuries / Q000235:genetics / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010636:Phenols; D017382:Reactive Oxygen Species; C011006:jasmonic acid; C036216:ethylene