title: Fluorescence-guided surgery of retroperitoneal-implanted human fibrosarcoma in nude mice delays or eliminates tumor recurrence and increases survival compared to bright-light surgery.
abstract: The aim of this study is to determine if fluorescence-guided surgery (FGS) can eradicate human fibrosarcoma growing in the retroperitoneum of nude mice. One week after retroperitoneal implantation of human HT1080 fibrosarcoma cells, expressing green fluorescent protein (GFP) (HT-1080-GFP), in nude mice, bright-light surgery (BLS) was performed on all tumor-bearing mice (n = 22). After BLS, mice were randomized into 2 treatment groups; BLS-only (n = 11) or the combination of BLS + FGS (n = 11). The residual tumors remaining after BLS were resected with FGS using a hand-held portable imaging system under fluorescence navigation. The average residual tumor area after BLS + FGS was significantly smaller than after BLS-only (0.4 ± 0.4 mm(2) and 10.5 ± 2.4 mm(2), respectively; p = 0.006). Five weeks after surgery, the fluorescent-tumor areas of BLS- and BLS + FGS-treated mice were 379 ± 147 mm(2) and 11.7 ± 6.9 mm(2), respectively, indicating that FGS greatly inhibited tumor recurrence compared to BLS. The combination of BLS + FGS significantly decreased fibrosarcoma recurrence compared to BLS-only treated mice (p < 0.001). Mice treated with BLS+FGS had a significantly higher disease-free survival rate than mice treated with BLS-only at five weeks after surgery. These results suggest that combination of BLS + FGS significantly reduced the residual fibrosarcoma volume after BLS and improved disease-free survival.
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America; Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Fuminari
authors.initials: F
authors.lastname: Uehara
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hiroshima
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America; Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America.
authors.forename: Mako
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maehara
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kanaya
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, United States of America; Department of Surgery, University of California San Diego, 200 West Arbor Drive, San Diego, California 92103, United States of America.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology / Q000601:surgery*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009364:Neoplasm Recurrence, Local / Q000209:etiology / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*; D012186:Retroperitoneal Neoplasms / Q000473:pathology / Q000601:surgery*; D025321:Surgery, Computer-Assisted / Q000009:adverse effects / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins