title: No Overlap among Serum GAD65, NMDAR and AQP4 Antibodies in Patients with Neuromyelitis Optica Spectrum Disorders.
abstract: OBJECTIVE
  To evaluate whether serum glutamic acid decarboxylase (GAD), N-methyl-D-aspartate-receptor (NMDAR), and aquaporin-4 (AQP4) autoantibodies coexist in patients with neuromyelitis optica (NMO)/NMO spectrum disorders (NMOSD).
  
  
  METHODS
  Serum samples were collected from 98 patients with NMO/NMOSD. Serum GAD65, NMDAR and AQP4 antibodies were measured using a cell-based assay.
  
  
  RESULTS
  A total of 63 patients (64.3%) had myelitis and optic neuritis and satisfied the revised diagnostic criteria for NMO. Longitudinally extensive transverse myelitis was seen on spinal cord magnetic resonance imaging, showing continuous T2-weighted signal abnormalities in at least three vertebral segments in 26 patients (26.5%); 5 patients (5.1%) had recurrent optic neuritis, and 4 patients (4.1%) had brain syndromes with optic neuritis and myelitis. None of the 98 patients had diabetes, stiff-man syndrome, or epilepsy. All 98 patients tested positive for AQP4 antibody. No patients tested positive for GAD65 and NMDAR antibodies.
  
  
  CONCLUSIONS
  In the present study, we found no simultaneous presence of serum GAD65, NMDAR and AQP4 antibodies in patients with NMO/NMOSD.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Longchang
authors.initials: L
authors.lastname: Xie
authors.forename: Youming
authors.initials: Y
authors.lastname: Long
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Fulan
authors.initials: F
authors.lastname: Shan
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Fan
authors.forename: Rong
authors.initials: R
authors.lastname: Zhong
authors.forename: Linzhan
authors.initials: L
authors.lastname: Wu
authors.forename: Jianrui
authors.initials: J
authors.lastname: Yin
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Gao
authors.forename: Gao
authors.initials: G
authors.lastname: Cong
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000097:blood*; D051401:Aquaporin 4 / Q000276:immunology*; D001921:Brain / Q000473:pathology; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008875:Middle Aged; D009471:Neuromyelitis Optica / Q000097:blood* / Q000473:pathology; D016194:Receptors, N-Methyl-D-Aspartate / Q000276:immunology*; D055815:Young Adult
chemical_list: C496030:AQP4 protein, human; D000906:Antibodies; D051401:Aquaporin 4; D016194:Receptors, N-Methyl-D-Aspartate; C505804:GAD55, human; D005968:Glutamate Decarboxylase