title: Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification.
abstract: Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.
authors.affiliation: Department of Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kobylak
authors.affiliation: Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bykowska
authors.affiliation: Department of Dermatology, Venereology and Allergology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Nowicki
authors.affiliation: Department of Microbiology, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Brillowska-Dąbrowska
mesh_terms: D003883:Arthrodermataceae / Q000302:isolation & purification*; D006358:Hot Temperature; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014249:Trichophyton / Q000145:classification / Q000302:isolation & purification*