title: Effects of long-term dietary supplementation with conjugated linoleic acid on bovine oocyte lipid profile.
abstract: Nutritional and environmental conditions around conception and during early embryonic development may have significant effects on health and well-being in adult life. Here, a bovine heifer model was used to investigate the effects of rumen-protected fat supplementation on oocyte quality and embryo development. Holstein-Friesian heifers (n = 84) received a dietary supplement consisting of rumen-protected conjugated linoleic acid (CLA) or stearic acid (SA), each on top of an isocaloric basic diet. Oocytes were collected via ultrasound-guided follicular aspiration and subjected to in vitro maturation followed by either desorption electrospray ionisation mass spectrometry (DESI-MS) for lipid profiling of individual oocytes or in vitro fertilisation and embryo culture. The type of supplement significantly affected lipid profiles of in vitro-matured oocytes. Palmitic acid and plasmalogen species were more abundant in the mass spectra of in vitro-matured oocytes after rumen-protected SA supplementation when compared with those collected from animals supplemented with CLA. Lipid concentrations in blood and follicular fluid were significantly affected by both supplements. Results show that rumen-protected fatty-acid supplementation affects oocyte lipid content and may pave the way for the establishment of a large-animal model for studies towards a better understanding of reproductive disorders associated with nutritional impairments.
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Andrés F
authors.initials: AF
authors.lastname: González-Serrano
authors.affiliation: Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, 207 South Martin Jischke Drive, West Lafayette, 47907 IN, USA.
authors.forename: Christina R
authors.initials: CR
authors.lastname: Ferreira
authors.affiliation: Department of Chemistry, University of Turin, Via Pietro Giuria 7, 10125 Turin, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pirro
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lucas-Hahn
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Heinzmann
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Klaus-Gerd
authors.initials: KG
authors.lastname: Hadeler
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Baulain
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Aldag
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), Bundesallee 50, 38116 Braunschweig, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Meyer
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Piechotta
authors.affiliation: Institute of Nutrition, Friedrich Schiller University of Jena, Dornburger Strasse 25, 07743 Jena, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Jahreis
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), Bundesallee 50, 38116 Braunschweig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
authors.affiliation: Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, 207 South Martin Jischke Drive, West Lafayette, 47907 IN, USA.|Corresponding authors. Emails.
authors.forename: R Graham
authors.initials: RG
authors.lastname: Cooks
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Hoelty Strasse 10, 31535 Neustadt, Germany.|Corresponding authors. Emails.
authors.forename: Heiner
authors.initials: H
authors.lastname: Niemann