title: Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis.
abstract: Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, UK. e.fullam@warwick.ac.uk.
authors.forename: R
authors.initials: R
authors.lastname: Lowery
authors.forename: M I
authors.initials: MI
authors.lastname: Gibson
authors.forename: R L
authors.initials: RL
authors.lastname: Thompson
authors.forename: E
authors.initials: E
authors.lastname: Fullam
mesh_terms: D001692:Biological Transport / Q000502:physiology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry*; D020102:Mycobacterium smegmatis / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D003903:Deuterium