title: Stress enhances the sensitivity of Salmonella enterica serovar Typhimurium to bacteriocins.
abstract: OBJECTIVE
  The aim of this study was to evaluate the potential application of bacteriocins against Gram-negative bacteria when associated with others food preservation methods.
  
  
  RESULTS
  Salmonella was subjected to heat, cold, acid and chemical (with ethylenediaminetetracetate and trisodium phosphate) stresses. Then, the cells were recovered and subjected to treatment with bacteriocins (500 AU ml(-1) ) for 6 h. Heat and cold stress were those that promoted more sensitization to bactericidal activity of nisin. Under the same conditions, bovicin HC5 acted more rapidly than nisin reducing the number of viable cells to undetectable levels after 20 min of treatment. Similar results with use of nisin only were observed after 6 h of treatment.
  
  
  CONCLUSIONS
  Stress conditions used in food industry, such as temperature and pH, and use of chelating agents or membrane disruptors, sensitized Salmonella Typhimurium cells to bacteriocins produced by lactic acid bacteria, such as nisin and bovicin HC5.
  
  
  CONCLUSIONS
  Food preservation methods sensitized Gram-negative bacteria to bacteriocins activity, which demonstrate the potential of nisin and bovicin HC5 to inhibit the growth of Salmonella.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Galvão
authors.forename: C V
authors.initials: CV
authors.lastname: Prudêncio
authors.forename: M C D
authors.initials: MC
authors.lastname: Vanetti
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin; C102526:nisin A
keywords: Salmonella; biocontrol; bovicin HC5; food industry; hurdle technology; nisin; outer membrane