title: Evaluation of fibroblast attachment in root conditioning with Er, Cr:YSGG laser versus EDTA: a SEM study.
abstract: The regeneration of periodontal support is a main concern in periodontal therapy. This study aims to investigate the efficacy of Er, Cr:YSGG laser and EDTA based conditioning in attachment of fibroblast on root surfaces. This in vitro study was conducted on 81 root plates (6 mm × 4 mm × 1 mm) prepared from 27 single-rooted human mature teeth. The samples were divided into three groups: (1) Er, Cr: YSGG laser conditioning with a G6 tip (2.78 µm, 0.75 W, pulse duration of 140 µs, repetition rate of 20 Hz) for 5-7 s; (2) EDTA conditioning (17%, pH: 8) for 1 min; and (3) the control group which were exposed neither to EDTA nor laser. The viability and proliferation rates assessments were performed using MTT assay on days 3 and 5. In addition, the level of cell attachment was studied using scanning electron microscopy. The data indicated Er, Cr:YSGG conditioning increased cell viability by lapse of time (from days 3-5), with significantly better cell attachment compared to the other groups on days 3 and 5 (P < 0.05). In addition, increasing cell attachment in the EDTA conditioning group compared with the control group was statistically significant on day 5 but not on day 3 (P < 0.05). In conclusion, Er, Cr:YSGG laser conditioning can promote enhance fibroblast attachment on dentinal root surfaces more than EDTA.
authors.affiliation: Laser Research Center of Dentistry, Dental Faculty, Tehran University of Medical Sciences, Periodontal Department, Dental Faculty, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Fekrazad
authors.forename: Ghogha
authors.initials: G
authors.lastname: Lotfi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Harandi
authors.forename: Sara
authors.initials: S
authors.lastname: Ayremlou
authors.forename: Katayoun A M
authors.initials: KA
authors.lastname: Kalhori
mesh_terms: D002448:Cell Adhesion; D002470:Cell Survival; D004492:Edetic Acid / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology*; D005562:Formazans / Q000032:analysis; D006801:Humans; D053844:Lasers, Solid-State; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D018915:Root Canal Preparation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts / Q000032:analysis; D014016:Tissue Conditioning, Dental / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D004492:Edetic Acid
keywords: EDTA; Er, Cr:YSGG laser; root conditioning; scanning electron microscopy