title: Characterizing protein modifications by reactive metabolites using magnetic bead bioreactors and LC-MS/MS.
abstract: We report here label-free metabolite-protein adduct detection and identification employing magnetic beads coated with metabolic enzymes as bioreactors to generate metabolites and possible metabolite-protein adducts for analysis by liquid chromatography-tandem mass spectrometry.
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, USA. james.rusling@uconn.edu.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.forename: You-Jun
authors.initials: YJ
authors.lastname: Fu
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
mesh_terms: D019149:Bioreactors; D002853:Chromatography, Liquid; D051549:Glutathione S-Transferase pi / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D060328:Magnetic Phenomena; D008863:Microspheres; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry
chemical_list: D051549:Glutathione S-Transferase pi