title: Characterization of multidrug-resistant diabetic foot ulcer enterococci.
abstract: BACKGROUND
  Diabetes mellitus is a highly prevalent chronic progressive disease with complications that include diabetic-foot ulcers.
  
  
  METHODS
  Enterococci isolated from diabetic-foot infections were identified, evaluated by macro-restriction analysis, and screened for virulence traits and antimicrobial resistance.
  
  
  RESULTS
  All isolates were considered multidrug-resistant, cytolysin and gelatinase producers, and the majority also demonstrated the ability to produce biofilms.
  
  
  CONCLUSIONS
  These results indicate the importance of enterococci in diabetic-foot infection development and persistence, especially regarding their biofilm-forming ability and resistance to clinically relevant antibiotics.
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal. Electronic address: tlemsaddek@fmv.ulisboa.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Semedo-Lemsaddek
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Mottola
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Alves-Barroco
authors.affiliation: TechnoPhage, S.A., Lisboa, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Monte de Caparica, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Cavaco-Silva
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: Tavares
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Oliveira
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms; D017719:Diabetic Foot / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D016983:Enterococcus / Q000187:drug effects* / Q000472:pathogenicity; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents
keywords: Biofilms; Diabetic foot infections; Enterococcus; Infecciones del pie diabético; Marcadores de virulencia; Multi-drug-resistant (MDR); Multirresistencia; Virulence factors