title: Multipotent Adult Progenitor Cells from Bone Marrow Differentiate into Chondrocyte-Like Cells.
abstract: Cartilage tissue engineering has great potential for treating chondral and osteochondral injuries. Efficient seed cells are the key to cartilage tissue engineering. Multipotent adult progenitor cells (MAPCs) have greater differentiation ability than other bone-marrow stem cells, and thus may be candidate seed cells. We attempted to differentiate MAPCs into chondrocyte-like cells to evaluate their suitability as seed cells for cartilage tissue engineering. Toluidine blue and Alcian blue staining suggested that glycosaminoglycan was expressed in differentiated cells. Immunofluorostaining indicated that differentiated human MAPCs (hMAPCs) expressed collagen II. Based on these results, we concluded that bone-marrow-derived hMAPCs could differentiate into chondrocyte-like cells in vitro.
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
authors.forename: Lele
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China. Electronic address: drwengym@163.com.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Shui
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
authors.forename: Wenlai
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
authors.forename: Erge
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
mesh_terms: D000423:Alcian Blue / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology*; D002356:Cartilage / Q000473:pathology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D024043:Collagen Type II / Q000737:chemistry; D005434:Flow Cytometry; D006025:Glycosaminoglycans / Q000737:chemistry; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D014048:Tolonium Chloride / Q000737:chemistry
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; D014048:Tolonium Chloride; D000423:Alcian Blue
keywords: MAPC; cartilage tissue engineering; chondrocyte; differentiation; multipotent adult progenitor cells