title: Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry.
abstract: Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ling-Na
authors.initials: LN
authors.lastname: Zheng
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Lei-Chao
authors.initials: LC
authors.lastname: Zhao
authors.forename: Bao-Yun
authors.initials: BY
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Chen
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Zhao
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Chai
authors.forename: Wei-Yue
authors.initials: WY
authors.lastname: Feng
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000378:metabolism*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000032:analysis / Q000378:metabolism*; D005682:Gadolinium / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism*; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D005682:Gadolinium; D002945:Cisplatin