Novel monoclonal antibody against alphaX subunit from horse CD11c/CD18 integrin.
The αX I-domain of the horse integrin CD11c was successfully expressed in Escherichia coli, purified, biochemically characterized and used as immunogen to generate murine monoclonal antibodies against horse CD11c, which are not yet commercially available. One monoclonal antibody mAb-1C4 against the αX I-domain, is an IgG2a able to interact with the recombinant I-domain, showing an EC50=2.4ng according to ELISA assays. By western blot with horse PBMCs lysates the mAb-1C4 recognized a protein of 150kDa which corresponds well with the CD11c molecule. Using immunohistochemistry in horse lymph node tissue sections, mAb-1C4 marked cells in situ, some with apparent dendritic morphology. Thus the mAb generated to a recombinant epitope from horse CD11c identified the molecule in intact cells within horse lymphoid tissue. By the labelling intensity, the histological location (paracortical and interfollicular areas) and the apparent morphology of the marked cells, we can say that these are putative horse dendritic cells (DCs). The development of a mAb to horse CD11c provides a new tool to better study the horse DC biology and opens other biotechnological avenues, such as DC targeting-based vaccines.
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia - Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Colonia Chamilpa, Apartado Postal 510-3, Cuernavaca, Morelos, Mexico. Electronic address: Gerardo.Espino-Solis@baylorhealth.edu.
Gerardo Pavel
GP
Espino-Solis
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia - Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Colonia Chamilpa, Apartado Postal 510-3, Cuernavaca, Morelos, Mexico.
Veronica
V
Quintero-Hernandez
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia - Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Colonia Chamilpa, Apartado Postal 510-3, Cuernavaca, Morelos, Mexico.
Alejandro
A
Olvera-Rodriguez
Departamento de Biologia Celular CINVESTAV-IPN, Av IPN 2508, San Pedro Zacatenco, Mexico, D.F., Mexico.
Juana
J
Calderon-Amador
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia - Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Colonia Chamilpa, Apartado Postal 510-3, Cuernavaca, Morelos, Mexico.
Martha
M
Pedraza-Escalona
Departamento de Biotecnologia Marina, C.I.C.E.S.E. Carretera Ensenada-Tijuana # 3918, Zona Playitas, Ensenada, B.C., Mexico.
Alexei
A
Licea-Navarro
Departamento de Biologia Celular CINVESTAV-IPN, Av IPN 2508, San Pedro Zacatenco, Mexico, D.F., Mexico.
Leopoldo
L
Flores-Romo
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia - Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Colonia Chamilpa, Apartado Postal 510-3, Cuernavaca, Morelos, Mexico. Electronic address: possani@ibt.unam.mx.
Lourival Domingos
LD
Possani
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D039521:CD11c Antigen / Q000737:chemistry / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D039521:CD11c Antigen; D018821:CD18 Antigens
CD11c integrin; Dendritic cells; In situ; Monoclonal antibodies