Continuous acidogenesis of sucrose, raffinose and vinasse using mineral kissiris as promoter.
The use of kissiris as promoter (culture immobilization carrier) in anaerobic acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4-8) and fermentation temperature (18-52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5-1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
Food Biotechnology Group, Department of Chemistry, University of Patras, 26500 Patras, Greece.
Katerina
K
Lappa
Food Biotechnology Group, Department of Chemistry, University of Patras, 26500 Patras, Greece.
Panagiotis
P
Kandylis
Food Biotechnology Group, Department of Chemistry, University of Patras, 26500 Patras, Greece.
Argyro
A
Bekatorou
B.G. Spiliopoulos, 87-89 Akti Dimeon, 26333 Patras, Greece.
Nikolaos
N
Bastas
B.G. Spiliopoulos, 87-89 Akti Dimeon, 26333 Patras, Greece.
Stavros
S
Klaoudatos
I. Athanasopoulos and Co, 106b Lontou, 26224 Patras, Greece.
Nikolaos
N
Athanasopoulos
Food Biotechnology Group, Department of Chemistry, University of Patras, 26500 Patras, Greece.
Maria
M
Kanellaki
Food Biotechnology Group, Department of Chemistry, University of Patras, 26500 Patras, Greece. Electronic address: a.a.koutinas@upatras.gr.
Athanasios A
AA
Koutinas
D001419:Bacteria / Q000378:metabolism; D056804:Biofuels; D019149:Bioreactors; D018914:Cells, Immobilized; D002851:Chromatography, High Pressure Liquid; D045424:Complex Mixtures / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste; D019344:Lactic Acid / Q000737:chemistry; D008903:Minerals / Q000737:chemistry*; D011887:Raffinose / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*; D013696:Temperature
D056804:Biofuels; D045424:Complex Mixtures; D003470:Culture Media; D004952:Esters; D007220:Industrial Waste; D008903:Minerals; D019344:Lactic Acid; D000431:Ethanol; D013395:Sucrose; D012822:Silicon Dioxide; D011887:Raffinose
Acidogenesis; Biofuel; Raffinose; Sucrose; Vinasse