A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out.
A luminescent probe for determination of pH was designed based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.
Robert J
RJ
Meier
Johann M B
JM
Simbürger
Tero
T
Soukka
Michael
M
Schäferling
D002614:Chelating Agents / Q000737:chemistry; D005063:Europium / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D015394:Molecular Structure
D002614:Chelating Agents; D005456:Fluorescent Dyes; D005063:Europium