Immobilization of α-amylase onto poly(glycidyl methacrylate) grafted electrospun fibers by ATRP.
In this study, novel α-amylase immobilized poly(vinyl alcohol) (PVA) nanofibers were prepared. The PVA nanofiber surfaces were functionalized with 2-bromoisobutyryl bromide (BiBBr) and followed by surface initiated atom transfer radical polymerization (SI-ATRP) of glycidyl methacrylate (GMA). The morphology of the poly(glycidyl methacrylate) (PGMA) grafted PVA nanofibers was characterized by scanning electron microscopy (SEM). Also PGMA brushes were confirmed by X-ray photo electron microscopy (XPS). α-Amylase was immobilized in a one step process onto the PGMA grafted PVA nanofiber. The characteristic properties of the immobilized and free enzymes were examined. The thermal stability of the enzyme was improved and showed maximum activity at 37 °C by immobilization. pH values of the maximum activity of the free and immobilized enzymes were also found at 6.0 and 6.5, respectively. Free enzyme lost its activity completely within 15 days. The immobilized enzyme lost only 23.8% of its activity within 30 days.
Marmara University, Department of Chemistry, 34722 Istanbul, Turkey.
Burcu
B
Oktay
Marmara University, Department of Chemistry, 34722 Istanbul, Turkey.
Serap
S
Demir
Marmara University, Department of Chemistry, 34722 Istanbul, Turkey. Electronic address: napohan@marmara.edu.tr.
Nilhan
N
Kayaman-Apohan
D000818:Animals; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D059027:Recycling; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D013696:Temperature; D013818:Thermogravimetry; D000516:alpha-Amylases / Q000378:metabolism*
D004800:Enzymes, Immobilized; D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate; D011142:Polyvinyl Alcohol; D000516:alpha-Amylases
Electrospun nanofiber; Enzyme immobilization; Polymer brushes; Reactive electrospinning; Surface initiated atom transfer radical polymerization