The effect of mitochondrial ATP-sensitive potassium channels on apoptosis of chick embryo cecal cells by Eimeria tenella.
The objective of this study was to investigate the effect of mitochondrial ATP-sensitive potassium (mitoKATP) channels on apoptosis induced by Eimeria tenella. At 24, 48, 72, 96 and 120 h after Eimeria tenella infection, TUNEL assays and translation of phosphatidyl serines to the host cell plasma membrane surface showed that diazoxide-treated chick embryo cecal cells underwent less apoptosis (P <0.05), while light microscopy showed that infection rates of treated cells were higher (P <0.01) than untreated cells. Caspase 9 and caspase 3 of infected cells were activated less (P <0.01) in diazoxide-treated cells than untreated cells. These results indicate that opening mitoKATP channels can protect chick embryo cecal cells from mitochondria-dependent apoptosis induced by Eimeria tenella by inhibiting activations of caspase 9 and caspase 3.
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Sha-sha
SS
Yang
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. Electronic address: zhengmingxue288@163.com.
Ming-xue
MX
Zheng
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Huan-cheng
HC
Xu
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Xiao-zhen
XZ
Cui
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Yan
Y
Zhang
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Wen-long
WL
Zhao
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.
Rui
R
Bai
D000818:Animals; D017209:Apoptosis; D002432:Cecum / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003048:Coccidiosis / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D003981:Diazoxide / Q000633:toxicity; D016786:Eimeria tenella / Q000502:physiology*; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D011201:Poultry Diseases / Q000378:metabolism / Q000469:parasitology*
D015221:Potassium Channels; C117780:mitochondrial K(ATP) channel; D003981:Diazoxide
Apoptosis; Chick embryo cecal cell; Eimeria tenella; Mitochondrial ATP-sensitive potassium channel