Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10.
The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial strain Streptomyces chattanoogensis L10. WhiGch regulates the growth and morphological differentiation of S. chattanoogensis L10. The whiG ch deletion mutant decreased natamycin production by about 30 % and delayed natamycin production more than 24 h by delaying the growth. Overexpression of the whiG ch gene increased natamycin production in large scale production medium by about 26 %. WhiGch upregulated the transcription of natamycin biosynthetic gene cluster and inhibited the expression of migrastatin and jadomycin analog biosynthetic polyketide synthase genes. WhiGch positively regulated natamycin biosynthetic gene cluster by directly binding to the promoters of scnC and scnD, which were involved in natamycin biosynthesis, and these binding sites adjacent to translation start codon were determined. Thus, this paper further elucidates the high natamycin yield mechanisms of industrial strains and demonstrates that a valuable improvement in the yield of the target metabolites can be achieved through manipulating the transcription regulators.
College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Shui-Ping
SP
Liu
Pin
P
Yu
Peng-Hui
PH
Yuan
Zhen-Xing
ZX
Zhou
Qing-Ting
QT
Bu
Xu-Ming
XM
Mao
Yong-Quan
YQ
Li
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D053938:DNA Fragmentation; D005285:Fermentation; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D046228:Microarray Analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D005810:Multigene Family; D010866:Natamycin / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D012808:Sigma Factor; D010866:Natamycin