Significance of Penicillium ochrochloron chitinase as a biocontrol agent against pest Helicoverpa armigera.
Penicillium ochrochloron chitinase purified by DEAE-cellulose ion exchange chromatography was evaluated for its antifeedant and growth inhibitory activities against Helicoverpa armigera at different concentrations of 2000, 1000, 500, 250 and 100 U mL(-1). It reduced the successful pupation and increased larval and pupal mortality, adult emergence in a dosage-dependent manner when applied topically. The highest mortalities were recorded for groups treated with 2000 U mL(-1) chitinase activity. The studies showed P.ochrochloron chitinase can affect the growth of H.armigera larvae. Since this insect pest species has developed resistance and resurgence to chemical insecticides, only alternate is the usage of enzyme-based pesticide formulations as an environmentally friendly pest management tool.
Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
Nilambari S
NS
Patil
Department of Biotechnology, Shivaji University, Kolhapur 416004, India; Department of Biochemistry, Shivaji University, Kolhapur 416004, India. Electronic address: jpjbiochem@gmail.com.
Jyoti P
JP
Jadhav
D000818:Animals; D002688:Chitinases / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects*; D010407:Penicillium / Q000201:enzymology*; D010571:Pest Control / Q000379:methods*; D010575:Pesticides / Q000494:pharmacology*; D011679:Pupa / Q000187:drug effects
D010575:Pesticides; D002688:Chitinases
Biocontrol; Chitinase; Helicoverpa armigera; Penicillium ochrochloron