Effect of respiratory syncytial virus on the growth of hepatocellular carcinoma cell-lines.
In several reports, the respiratory syncytial virus (RSV) was identified as an oncolytic virus in cancer cells (e.g., lung and prostate cancer). However, the effects of RSV in hepatocellular carcinoma (HCC) cells have not yet been investigated. Here, we observed the inhibitory effects of RSV infection in HCC cell-lines. Cell growth was significantly decreased by RSV infection in BNL-HCC, Hep3B, Huh-7 and SNU-739 cells. After RSV infection, plaque formation and syncytial formation were observed in affected Hep3B and Huh-7 cells. RSV protein-expression was also detected in Hep3B and Huh-7 cells; however, only Huh-7 cells showed apoptosis after RSV infection. Furthermore, inhibition of cell migration by RSV infection was observed in BNL-HCC, Hep3B, Huh-7 and SNU-739 cells. Therefore, further investigation is required to clarify the molecular mechanism of RSV-mediated inhibition of HCC cell growth, and to develop potential RSV oncolytic viro-therapeutics.
Departments of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Korea.
Song Hee
SH
Choi
Departments of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Korea.
Byoung Kwon
BK
Park
Departments of Biomedical Science, College of Natural Science, Hallym University, Chuncheon 24252, Korea.
Keun-Wook
KW
Lee
Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Korea.
Jun
J
Chang
Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea.
Younghee
Y
Lee
Departments of Microbiology, College of Medicine, Hallym University, Chuncheon 24252; Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Korea.
Hyung-Joo
HJ
Kwon
D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology / Q000628:therapy* / Q000821:virology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy* / Q000821:virology*; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses / Q000502:physiology; D012136:Respiratory Syncytial Viruses / Q000502:physiology*