In vitro evaluation of anti-herpes simplex-1 activity of three standardized medicinal plants from Lamiaceae.
BACKGROUND
Rosmarinic acid (RA) is a phenolic acid with antioxidant and anti-viral effects. We have studied anti-herpes simplex virus type 1 (HSV-1) effect of three medicinal plants from Lamiaceae family which have been standardized on the basis of RA content.


METHODS
Methanolic extract of Teucrium polium, Ziziphora clinopoides, and Salvia rhytidea was prepared by maceration method and RA content of the plants was determined using a spectrophotometric method. Maximum nontoxic concentration (MNTC) of the extracts was determined using neutral red method. Serial dilutions of extracts up to MNTC were examined on Vero cells for anti-HSV-1 effect by plaque assay in comparison to acyclovir as a positive control.


RESULTS
Among the tested extracts, T. polium contained the highest percentage of RA (1.8%w/w) and exhibited the least toxicity (MNTC = 1000 μg/ml). The greatest anti-HSV-1 was shown by T. polium and Z. clinopoides extracts which exhibited both time and concentration-dependent plaque inhibition.


CONCLUSIONS
Considering the low toxicity and significant anti-viral effect of T. polium extract, this plant would prove valuable as an active anti-viral drug.
Pharmaceutics Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Mehdi
M
Ansari
Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Fariba
F
Sharififar
Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Ali Mohammad
AM
Arabzadeh
Herbal and Traditional Medicines Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Firoozeh
F
Mehni
International Centers for Science and Technology and Environmental Sciences (HITEC), Kerman, Iran.
Manosur
M
Mirtadzadini
Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Zahra
Z
Iranmanesh
Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Najmeh
N
Nikpour
Herpes simplex-1; Salvia rhytidea; Teucrium polium; Ziziphora clinopoides; plaque inhibition; rosmarinic acid