Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality.
Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate. These cells were also subjected to immunophenotypic characterization for the presence of CD90, CD105, and CD79, and the absence of CD45, CD34, and CD73, which are positive and negative markers of MSC, respectively. To prove their multipotency, the cells were induced to differentiate into three different cell types, chondrocytes, osteoblasts, and adipocytes, which were stained with tissue-specific dyes (Chondrogenic-Alcian Blue, Osteogenic-Alizarin Red, and Adipogenic-Oil-Red O, respectively) to confirm differentiation. Gene expression analysis of pluripotency-related genes was also conducted. Our results suggest that adipose tissue from bovines and buffalos can be used as a source of MSC, making adipose tissue-derived cells an interesting option for cell therapy and regenerative medicine. Additionally, these findings have implications for reproductive biotechnology because the use of MSC as nuclear donors has been linked to an increase in the efficiency of nuclear transfer.
sampaiorv@gmail.com.
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Sampaio
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
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MR
Chiaratti
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Santos
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
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Bressan
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
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Sangalli
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Sá
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Silva
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Costa
Instituto Federal de Ciência e Tecnologia do Pará, Belém, PA, Brasil.
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Cordeiro
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Santos
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
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Ambrosio
Universidade Norte do Paraná, Londrina, PR, Brasil.
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Adona
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
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Meirelles
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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MS
Miranda
Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
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Ohashi
D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002020:Buffaloes; D002417:Cattle; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D020219:Chondrogenesis; D016130:Immunophenotyping; D039902:Multipotent Stem Cells / Q000166:cytology*; D010012:Osteogenesis