Enhancing osteoblast-affinity of titanium scaffolds for bone engineering by use of ultraviolet light treatment.
Ultraviolet (UV) treatment immediately prior to use is attracting attention as an effective surface conditioning method for titanium to improve osteoblast-affinity. The affinity of titanium to osteoblasts in two-dimensional plate culture has been well studied, but that in three-dimensional cultures remains unclear. Here, we examined the effect of UV treatment on titanium scaffolds, comprising micro-thin titanium fibers, used in bone engineering. Titanium scaffolds, with and without UV treatment, were seeded with rat bone marrow derived osteoblasts, and the number of cells attached to scaffolds and osteoblastic phenotype in the cultures were examined. UV treatment improved the wettability of scaffolds and significantly reduced the percentage of surface carbon. Along with these physicochemical changes in the scaffolds, cell attachment increased by a factor of 1.3 as compared to that of the untreated control. In addition, alkaline phosphatase activity and calcium deposition significantly increased by a factor of 2.3 and 2.0, respectively. Robust formation of mineralized structures consisting of clear peaks of calcium and phosphorus was observed in the UV-treated scaffolds. The observed increase in osteoblast affinity and capability of mineralized matrix formation indicates the potential use of UV-treated titanium scaffolds for bone engineering.
Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku 101-8310; Laboratory of Bone and Implant Sciences (LBIS), Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, UCLA School of Dentistry, USA; Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.
Manabu
M
Ishijima
Pooya
P
Soltanzadeh
Makoto
M
Hirota
Naoki
N
Tsukimura
Tomohiko
T
Shigami
Takahiro
T
Ogawa
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion / Q000528:radiation effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000528:radiation effects; D008297:Male; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry / Q000528:radiation effects*; D014466:Ultraviolet Rays; D017155:Wettability
D015415:Biomarkers; C101914:titanium fiber; D014025:Titanium; D000469:Alkaline Phosphatase