Localization of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches.
Mutagenesis is the only process by which unpredicted biological gene function can be identified. Despite that several macroalgal developmental mutants have been generated, their causal mutation was never identified, because experimental conditions were not gathered at that time. Today, progresses in macroalgal genomics and judicious choices of suitable genetic models make mutated gene identification possible. This article presents a comparative study of two methods aiming at identifying a genetic locus in the brown alga Ectocarpus siliculosus: positional cloning and Next-Generation Sequencing (NGS)-based mapping. Once necessary preliminary experimental tools were gathered, we tested both analyses on an Ectocarpus morphogenetic mutant. We show how a narrower localization results from the combination of the two methods. Advantages and drawbacks of these two approaches as well as potential transfer to other macroalgae are discussed.
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Bernard
B
Billoud
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Émilie
É
Jouanno
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Zofia
Z
Nehr
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Baptiste
B
Carton
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Élodie
É
Rolland
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Sabine
S
Chenivesse
Sorbonne Université, UPMC University Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff CS 90074, F-29688, Roscoff cedex, France.
Bénédicte
B
Charrier
NGS-based mapping; SNP mapping; SSR; genetic locus; macroalga; mutant; positional cloning