An effective and convenient method for the delivery of apple latent spherical virus (ALSV)-based vectors into plant cells by agroinoculation.
Virus infection leads to the synthesis of double-stranded RNA during virus replication, and then this infection produces small RNA molecules in the antiviral RNA silencing pathway. Here, we develop an Agrobacterium-mediated inoculation system for ALSV-based vectors. This system is more effective and convenient for inoculation of the ALSV vectors into plants compared to direct inoculation of ALSV-RNA2-based vectors in pUC plasmids reported previously. In addition, cointroduction of various plant viral RNA-silencing suppressors increased the efficiency of agroinoculation of the ALSV-based vector. An ALSV vector could be successfully used to silence an endogenous gene in plants. Therefore, the ALSV-based VIGS/agroinoculation system provides a valuable tool for functional genomics among a broad range of plant species.
Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550, Japan.
Tatsuya
T
Kon
Nubuyuki
N
Yoshikawa
D060054:Agrobacterium / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics; D010944:Plants / Q000235:genetics / Q000382:microbiology* / Q000821:virology; D012328:RNA Viruses / Q000235:genetics*
D010940:Plant Proteins