Phosphate enhancing fermentative hydrogen production from substrate with municipal solid waste composting leachate as a nutrient.
To overcome phosphorus deficiency in municipal solid waste composting leachate, orthophosphate (OP) and pyrophosphate (PP) were separately added into leachate to evaluate the possibility of fermentative H2 production with leachate and phosphorus-rich streams as a full nutrient source. Results indicate H2 production is significantly promoted by OP addition but slightly facilitated by PP in some cases, depending on initial pH and P dosage. The highest hydrogen yield (1.95±0.07mol H2/mol glucose) was achieved at a COD/P ratio of 27.64 (mg/mg) with OP as phosphorus source at initial pH 5. For PP, a maximum yield of 1.58±0.09mol H2/mol glucose can be attained at the optimal COD/P ratio of 221.12 (mg/mg) and initial pH 5. OP promotes H2 production via dual approaches: supplying nutrient and relieving inhibition from excessive Ca(2+) on granule sludge. However, both the roles in nutrient supply and Ca(2+) removal by PP addition are limited.
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China; Shanghai Key Laboratory of Bio-energy Crops, Shanghai University, Shanghai 200444, China.
Qiang
Q
Liu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Wen
W
Chen
School of Civil, Environmental and Chemical Engineering, RMIT University, GPO Box 2476, Melbourne, VIC 3001, Australia.
Xiaolei
X
Zhang
Shanghai Pudong Environmental Protection Development Co. Ltd., No. 1229, Dongxiu Road, Shanghai 201207, China.
Lijia
L
Yu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Jizhi
J
Zhou
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China. Electronic address: yfxu@shu.edu.cn.
Yunfeng
Y
Xu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Guangren
G
Qian
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D002947:Cities; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000302:isolation & purification / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D012988:Soil Microbiology; D062611:Solid Waste; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*
D010710:Phosphates; D062611:Solid Waste; D014874:Water Pollutants, Chemical; D006859:Hydrogen; D005947:Glucose
Biohydrogen production; Fresh leachate; Orthophosphate; Precipitation; Pyrophosphate