Simultaneous analysis of Cr(III), Cr(VI), and chromium picolinate in foods using capillary electrophoresis-inductively coupled plasma mass spectrometry.
We herein reported a method for the simultaneous detection of trace Cr(VI), Cr(III), and chromium(III) picolinate (CrPic) in foods using CE-ICP-MS together with ultrasonic-assisted extraction. The Cr(III) (Cr(3+) ) was chelated with trans-1,2-diaminocyclohexane-N,N,N´,N´-tetraacetic acid (DCTA) to form a single charged Cr-DCTA(-) complex. Then, Cr(VI) (CrO4 (2-) ), Cr-DCTA(-) , and CrPic were separated by CE within 8 min under a separation voltage of -13 KV followed by their monitoring with ICP mass spectrometer (ICP-MS). The proposed method is simple, effective, and sensitive. It has an instrument detection limit of 0.10, 0.18, and 0.20 ngCr/mL for Cr(VI), Cr(III), and CrPic, respectively. With the help of the methods, we have successfully determined Cr(VI), Cr(III), and CrPic in nutritional supplement (CrPic yeast tablet) with an RSD (n = 5) <6% and a recovery of 93-103%. The experimental results showed that CrPic was the main speciation of chromium in the nutritional supplement, with a concentration of 1514.6 μg Cr/g.
Department of Chemistry, Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, P. R. China.
YiQuan
Y
Chen
Department of Chemistry, Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, P. R. China.
JinFa
J
Chen
Department of Chemistry, Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, P. R. China.
Zhiming
Z
Xi
Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou, Fujian, P. R. China.
Guidi
G
Yang
Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou, Fujian, P. R. China.
Zujian
Z
Wu
Food Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou, Liaoning, P. R. China.
JianRong
J
Li
Department of Chemistry, Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, P. R. China.
FengFu
F
Fu
D002857:Chromium / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D010848:Picolinic Acids / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results
D010848:Picolinic Acids; D002857:Chromium; C030614:picolinic acid
Capillary electrophoresis; Chromium; Chromium picolinate; ICP-MS; Speciation analysis