DNA/HSA interaction and nuclease activity of an iron(III) amphiphilic sulfonated corrole.
The DNA binding of amphiphilic iron(III) 2,17-bis(sulfonato)-5,10,15-tris(pentafluorophenyl)corrole complex (Fe-SC) was studied using spectroscopic methods and viscosity measurements. Its nuclease-like activity was examined by using pBR322 DNA as a target. The interaction of Fe-SC with human serum albumin (HSA) in vitro was also examined using multispectroscopic techniques. Experimental results revealed that Fe-SC binds to ct-DNA via an outside binding mode with a binding constant of 1.25 × 10(4) M(-1). This iron corrole also displays good activity during oxidative DNA cleavage by hydrogen peroxide or tert-butyl hydroperoxide oxidants, and high-valent (oxo)iron(V,VI) corrole intermediates may play an important role in DNA cleavage. Fe-SC exhibits much stronger binding affinity to site II than site I of HSA, indicating a selective binding tendency to HSA site II. The HSA conformational change induced by Fe-SC was confirmed by UV/Vis and CD spectroscopy.
Department of Chemistry, South China University of Technology, Guangzhou, 510640, China.
Yang
Y
Zhang
Department of Chemistry, South China University of Technology, Guangzhou, 510640, China.
Jin-Yan
JY
Wen
Department of Chemistry, South China University of Technology, Guangzhou, 510640, China.
Mian H R
MH
Mahmood
Department of Chemistry, South China University of Technology, Guangzhou, 510640, China.
Xiang-Li
XL
Wang
Department of Applied Phsics, South China University of Technology, Guangzhou, 510640, China.
Biao-Biao
BB
Lv
Department of Applied Phsics, South China University of Technology, Guangzhou, 510640, China.
Xiao
X
Ying
State Key Laboratory of Optoelectronics Materials and Technologies, Sun-Yat Sen University, Guangzhou, 510275, China.
Hui
H
Wang
State Key Laboratory of Optoelectronics Materials and Technologies, Sun-Yat Sen University, Guangzhou, 510275, China.|MOE Laboratory of Bioinorganic and Synthetic Chemistry, Sun-Yat Sen University, Guangzhou, 510275, China.
Liang-Nian
LN
Ji
Department of Chemistry, South China University of Technology, Guangzhou, 510640, China.
Hai-Yang
HY
Liu
D053837:DNA Cleavage; D004278:DNA, Superhelical / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D015394:Molecular Structure; D011166:Porphyrins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014783:Viscosity
D004278:DNA, Superhelical; D005290:Ferric Compounds; D011166:Porphyrins; D012709:Serum Albumin; D013451:Sulfonic Acids; D013501:Surface-Active Agents; C431863:corrole
DNA; HSA; corrole; iron; nuclease activity