A sensitive whole-cell biosensor for the simultaneous detection of a broad-spectrum of toxic heavy metal ions.
Bacterial biosensors are simple, cost-effective and efficient analytical tools for detecting bioavailable heavy metals in the environment. This work presents the design, construction and calibration of a novel whole-cell fluorescent biosensory device that, simultaneously and with high sensitivity, reports the presence of toxic mercury, lead, cadmium and/or gold ions in aqueous samples. This bio-reporter can be easily applied as an immediate alerting tool for detecting the presence of harmful pollutants in drinking water.
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR), Ocampo y Esmeralda, 2000-Rosario, Argentina. checa@ibr-conicet.gov.ar.
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D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002104:Cadmium / Q000032:analysis*; D002413:Cations, Divalent; D060766:Drinking Water / Q000737:chemistry*; D015870:Gene Expression; D017930:Genes, Reporter; D006046:Gold / Q000032:analysis*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007854:Lead / Q000032:analysis*; D008628:Mercury / Q000032:analysis*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis*
D002413:Cations, Divalent; D060766:Drinking Water; D014874:Water Pollutants, Chemical; C485192:green fluorescent protein, Aequorea victoria; D002104:Cadmium; D049452:Green Fluorescent Proteins; D007854:Lead; D006046:Gold; D008628:Mercury