Combining a sensor and a pH-gated nanopore based on an avidin-biotin system.
Here we propose a new approach to tailor nanopores, which combines both pH gating and sensing properties. This strategy is based on PEG like-avidin grafting in nanopores designed by atomic layer deposition (ALD). Below pH 5 the nanopore is blocked. We show that the PEG chains are at the origin of these properties.
Institut Européen des Membranes, UMR5635 ENSM UM2 CNRS, Place Eugène Bataillon, 34095 Montpellier cedex 5, France. sebastien.balme@univ-montp2.fr.
Mathilde
M
Lepoitevin
Gael
G
Nguyen
Mikhael
M
Bechelany
Emmanuel
E
Balanzat
Jean-Marc
JM
Janot
Sebastien
S
Balme
D001360:Avidin / Q000737:chemistry*; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002234:Carbodiimides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D055664:Electrochemical Techniques; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D058608:Nanopores; D011092:Polyethylene Glycols / Q000737:chemistry; D012996:Solutions; D005719:gamma-Globulins / Q000032:analysis*
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D012996:Solutions; D005719:gamma-Globulins; D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin