Physiological perturbations in juvenile cuttlefish Sepia officinalis induced by subchronic exposure to dissolved zinc.
Although cephalopod early life stage development often occurs in coastal areas where contamination is real and continuous, the physiological perturbations induced by contaminants have been rarely investigated. This study focused on the Zn as it is one of the trace metals the most concentrated in coastal waters, worldwide. As Zn-tolerance limits were unknown in juvenile Sepia officinalis, the aim of this study was to estimate the threshold inducing mortality during the 2-first weeks post-hatching, and to determine its sensitivity using digestive and immune enzymatic assays, as well as growth and behavior follow-up during the first 5weeks post-hatching. Our study highlighted a Zn-mortality threshold lying between 185 and 230μgl(-1), and growth reductions occurring after 5-week at 108μgl(-1) and above, associated with enzymatic perturbations. These results underline a relatively important sensitivity of juvenile cuttlefish to Zn, pointed out by a wide diversity of biomarkers.
Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France. Electronic address: charles.lepabic@gmx.fr.
Charles
C
Le Pabic
Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
Christelle
C
Caplat
Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
Jean-Paul
JP
Lehodey
School of Biological Sciences, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK.
Lorna
L
Dallas
Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
Noussithé
N
Koueta
D000818:Animals; D004784:Environmental Monitoring; D049833:Sepia / Q000502:physiology*; D013312:Stress, Physiological; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D015032:Zinc
Growth; Juvenile stage; Mollusc cephalopod; Predation behavior; Sepia officinalis; Zinc