Nanoparticles of copper stimulate angiogenesis at systemic and molecular level.
Copper is a key element affecting blood vessel growth and muscle development. However, the ions released from Cu salts are toxic. Given their specific physicochemical properties, nanoparticles of Cu (NanoCu) may have different bioactivity and affect the development of blood vessel and muscles in a different manner than Cu salts. The objective of the study was to evaluate the influence of NanoCu on embryo development and angiogenesis at the systemic and molecular level, in experiments using a chick embryo model. Fertilized chicken eggs were divided into a control group, and groups injected with a placebo, CuSO4 or NanoCu. Embryo development at the whole body level and molecular indices using an embryo chorioallantoic membrane model were measured during embryogenesis. The present study indicated for the first time that NanoCu have pro-angiogenic properties at the systemic level, to a greater degree than CuSO4 salt. The properties of NanoCu were confirmed at the molecular level, demonstrating significant effects on mRNA concentration and on mRNA gene expression of all pro-angiogenic and pro-proliferative genes measured herein.
Division of Poultry Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. nataliamroczek1@wp.pl.
Natalia
N
Mroczek-Sosnowska
Division of Nanobiotechnology, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. ewa_sawosz@sggw.pl.
Ewa
E
Sawosz
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg, Denmark. krish@sund.ku.dk.
Krishna Prasad
KP
Vadalasetty
Division of Poultry Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. monika_lukasiewicz@sggw.pl.
Monika
M
Łukasiewicz
Division of Poultry Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. jan_niemiec@sggw.pl.
Jan
J
Niemiec
Division of Nanobiotechnology, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. mateusz_wierzbicki@sggw.pl.
Mateusz
M
Wierzbicki
Division of Nanobiotechnology, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. marta_prasek@sggw.pl.
Marta
M
Kutwin
Division of Nanobiotechnology, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland. slawomir_jaworski@sggw.pl.
Sławomir
S
Jaworski
Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg, Denmark. ach@sund.ku.dk.
André
A
Chwalibog
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply; D003300:Copper / Q000737:chemistry*; D019327:Copper Sulfate / Q000737:chemistry / Q000633:toxicity; D047108:Embryonic Development / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D003300:Copper; D019327:Copper Sulfate