[Function of a calcium-dependent protein kinase gene Pscamk in Puccinia striiformis f. sp. tritici].
OBJECTIVE
To clone calcium-dependent protein kinase gene (camk) from Puccinia striiformis f. sp. tritici (Pst) and analyze its function.


METHODS
The cDNA full-length of Pscamk was isolated by using reverse transcriptional-PCR (RT-PCR), and gene expression profile at different morphological stages was analyzed via quantitative real-time--PCR (qRT-PCR). Pst urediospores were treated with CaMK suppressor KN-93 and germination rate was investigated.


RESULTS
A gene cDNA full-length with 1 620 bp was obtained and designated as Pscamk. qRT-PCR analysis showed Pscamk expression was highly induced in the early stages of Pst infection and reached the maximum at 6 h post inoculation (hpi) as 20.74-fold as that in the control (0 hpi). With increasing of the concentration of CaMK suppressor KN-93, germination rate of Pst urediospores was gradually decreased. The germination rate was reduced to 8.02%, only 12% of the control, under 1.4 μmol/L KN-93 treatment at 10 h after incubation at 9 degrees C.


CONCLUSIONS
Pscamk might play a role in germination and germ tube elongation of Pst urediospores. This study provides a basis for exploring pathogenesis of calcium signaling pathway during Pst infection.
Juan
J
Qin
Chuanming
C
Huang
Fuxin
F
He
Xiaoguo
X
Zhu
Yang
Y
Zhang
Zhensheng
Z
Kang
Jun
J
Guo
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000382:microbiology; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D005656:Fungal Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases