Improved production of reducing sugars from rice straw using crude cellulase activated with Fe₃O₄/alginate nanocomposite.
Effect of Fe3O4 nanoparticles (NPs) and Fe3O4/Alginate nanocomposites (NCs) have been investigated on production and thermostability of crude cellulase enzyme system obtained by newly isolated thermotolerant Aspergillus fumigatus AA001. Fe3O4 NPs and Fe3O4/Alginate NCs have been synthesized by co-precipitation method and characterized through various techniques. In presence of Fe3O4 NPs and Fe3O4/Alginate NCs, filter paper activity of crude cellulase was increased about 35% and 40%, respectively in 72 h as compared to control. Fe3O4/Alginate NCs treated crude enzyme was thermally stable up to 8h at 70°C and retained 56% of its relative activity whereas; control samples could retain only 19%. Further, the hydrolysis of 1.0% alkali treated rice straw using Fe3O4/Alginate NCs treated cellulase gave much higher sugar productivity than control at optimal condition. These findings may be utilized in the area of biofuels and biowaste management.
Department of Biotechnology, Allahabad Agriculture University (ICAR), Allahabad 211007, India; Department of Chemical Engineering, Indian Institute of Technology (IIT), Banaras Hindu University, Varanasi 221005, India.
Neha
N
Srivastava
Department of Applied Chemistry, Delhi Technological University, Shahbad Daulatpur, Main Bawana Road, Delhi 110042, India.
Jay
J
Singh
Department of Biotechnology, Allahabad Agriculture University (ICAR), Allahabad 211007, India.
Pramod W
PW
Ramteke
Department of Chemical Engineering, Indian Institute of Technology (IIT), Banaras Hindu University, Varanasi 221005, India.
P K
PK
Mishra
Department of Physics & Astrophysics, University of Delhi, Delhi 110007, India. Electronic address: 84.srivastava@gmail.com.
Manish
M
Srivastava
D000464:Alginates / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000302:isolation & purification / Q000378:metabolism; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D005290:Ferric Compounds / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006868:Hydrolysis; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012275:Oryza / Q000737:chemistry*; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014866:Waste Products; D014961:X-Ray Diffraction
D000464:Alginates; D002241:Carbohydrates; D005290:Ferric Compounds; D006603:Hexuronic Acids; D014866:Waste Products; C000499:ferric oxide; D020723:Glucuronic Acid; D002480:Cellulase
Cellulases; Fe(3)O(4) nanoparticle; Saccharification; Sugar productivity; Thermostability