Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing.
An effective enzymatic glucose biosensor was developed by immobilizing glucose oxidase on chitosan submicron particles synthesized from the gladius of Todarodes pacificus (GCSP). The chemically synthesized chitosan from gladius was pulverized to submicron particles by ball milling technique, which was further characterized and compared with the standard chitosan (SCS). The degree of deacetylation of GCSP was determined using FTIR spectroscopy which was comparable to the value of standard chitosan. The glucose oxidase (GOx) was immobilized over GCSP on porous zinc oxide/platinum nanoparticle (ZnO/Pt) based electrode. The morphological and structural properties of the electrodes were analyzed using scanning electron microscopy and X-ray diffraction analysis. The glucose sensing behavior of electrode was estimated using electrochemical analysis and showed an excellent analytical performance. The electrode ZnO/Pt/GCSP conjugated with GOx displayed high sensitivity (88.76 μA mM(-1) cm(-2)) with low detection limit in short response time. In addition, the very low value of Michaelis-Menten constant for GCSP based electrode contributes a better affinity of the electrode surface towards glucose oxidase.
Department of Advanced Zoology and Biotechnology, Loyola College, Chennai 600034, India.
J R
JR
Anusha
Department of Advanced Zoology and Biotechnology, Loyola College, Chennai 600034, India.
Albin T
AT
Fleming
Laser and Sensor Lab, Department of Electrical Engineering, Pusan National University, Gumjeong-Ku, Busan 609735, Republic of Korea.
Hee-Je
HJ
Kim
Department of Chemistry, Dongguk University-Seoul, Seoul 100-715, Republic of Korea.
Byung Chul
BC
Kim
Department of Chemistry, Dongguk University-Seoul, Seoul 100-715, Republic of Korea.
Kook-Hyun
KH
Yu
Department of Chemistry, Dongguk University-Seoul, Seoul 100-715, Republic of Korea. Electronic address: cjustinraj@gmail.com.
C Justin
CJ
Raj
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D049832:Decapodiformes / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D010984:Platinum / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
D004800:Enzymes, Immobilized; D010984:Platinum; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide
Biopolymers; Biosensors; Chitosan; Glucose oxidase; Submicron particles