Crystal structure of ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus.
The ATP binding cassette (ABC) transporters, represent one of the largest superfamilies of primary transporters, which are very essential for various biological functions. The crystal structure of ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus has been determined at 1.77 Å resolution. The crystal structure revealed that the protomer has two thick arms, (arm I and II), which resemble 'L' shape. The ATP-binding pocket is located close to the end of arm I. ATP molecule is docked into the active site of the protein. The dimeric crystal structure of ATP-binding subunit of ABC transporter from G. kaustophilus has been compared with the previously reported crystal structure of ATP-binding subunit of ABC transporter from Salmonella typhimurium.
Department of Studies in Physics, University of Mysore, Mysore 570 006, India.
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M
Manjula
Department of Studies in Microbiology, University of Mysore, Mysore 570 006, India.
K J
KJ
Pampa
Department of Studies in Physics, University of Mysore, Mysore 570 006, India.
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SM
Kumar
Aurigene Discovery Technologies Ltd., #39/40, KIADB Industrial Area, Hosur Road, Electronic City Phase-II, Bangalore 560100, India.
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S
Mukherjee
Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
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N
Kunishima
Department of Studies in Chemistry, University of Mysore, Mysore 570 006, India.
K S
KS
Rangappa
Department of Studies in Physics, University of Mysore, Mysore 570 006, India. Electronic address: lokanath@physics.uni-mysore.ac.in.
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Lokanath
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D056506:Geobacillus / Q000737:chemistry*; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D011487:Protein Conformation; D055503:Protein Multimerization; D016415:Sequence Alignment
D018528:ATP-Binding Cassette Transporters; D026923:Amino Acid Transport Systems, Basic; D001426:Bacterial Proteins; C043828:histidine permease, Bacteria; D000255:Adenosine Triphosphate
ABC transporter; ATP binding protein; Geobacillus kaustophilus; X-ray diffraction