1236 C/T and 3435 C/T polymorphisms of the ABCB1 gene in Mexican breast cancer patients.
MDR1, which is encoded by the ABCB1 gene, is involved in multidrug resistance (hydrophobic), as well as the elimination of xenotoxic agents. The association between ABCB1 gene polymorphisms and breast cancer risk in different populations has been described previously; however, the results have been inconclusive. In this study, we examined the association between polymorphisms 3435 C/T and 1236 C/T in the ABCB1 gene and breast cancer development in Mexican women according to their menopausal status and molecular classification. Molecular subtypes as well as allele and genotype frequencies were analyzed. A total of 248 women with initial breast cancer diagnosis and 180 ethnically matched, healthy, unrelated individuals were enrolled. Polymerase chain reaction-restriction fragment length polymorphism was performed to detect polymorphisms 3435 C/T and 1236 C/T in the ABCB1 gene. Premenopausal T allele carriers of the 3435 C/T polymorphism showed a 2-fold increased risk of breast cancer with respect to the reference and postmenopausal groups, as well as triple-negative expression regarding the luminal A/B molecular subrogated subtypes. In contrast, the CT genotype of the 1236 polymorphism was a protective factor against breast cancer. We conclude that the T allele carrier of the 3435 C/T polymorphism in the ABCB1 gene in combination with an estrogen receptor-negative status may be an important risk factor for breast cancer development in premenopausal women.
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico.
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SA
Gutierrez-Rubio
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico.
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A
Quintero-Ramos
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico.
A
A
Durán-Cárdenas
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico.
R A
RA
Franco-Topete
UMAE Hospital de Especialidades del Centro Médico Nacional de Occidente del IMSS Hospital de Especialidades, Instituto Mexicano del Seguro Social, Guadalajara, Mexico.
J M
JM
Castro-Cervantes
Instituto Jalisciense de Cancerologia SSJ, Guadalajara, Mexico.
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Oceguera-Villanueva
Departamento de Salud Pública, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
L M
LM
Jiménez-Pérez
Unidad de Investigación en Epidemiología Clínica, UMAE Hospital de Especialidades del Centro Médico Nacional de Occidente del IMSS, Guadalajara, Mexico.
L M A
LM
Balderas-Peña
UMAE Hospital de Especialidades del Centro Médico Nacional de Occidente del IMSS Hospital de Especialidades, Instituto Mexicano del Seguro Social, Guadalajara, Mexico.
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G
Morgan-Villela
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico.
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A
Del-Toro-Arreola
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Colonia Independencia, Guadalajara, Jalisco, Mexico daneri@cucs.udg.mx.
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A
Daneri-Navarro
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001943:Breast Neoplasms / Q000208:ethnology* / Q000235:genetics*; D016022:Case-Control Studies; D005006:Ethnicity; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008800:Mexico; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause; D017697:Premenopause; D012307:Risk Factors
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B