Cellular and biological evaluation and diagnostic immunohistochemistry of cytokeratin 15/19 expression in distinguishing cutaneous basal cell carcinoma.
Recent studies have investigated the expression of proliferative markers, but little is known about the expression of cytokeratin 15 and 19 in different histological subtypes of basal cell carcinoma (BCC). We conducted cellular, biological, clinicopathological and immunohistochemical analysis on the manifestations of 8 BCC by hematoxylin and eosin stain (H&E) staining and immunohistochemistry and reviewed relevant literature. Microscopically, the tumor cells were multiple remarkable foci of epidermolytic hyperkeratosis with large pleomorphic nuclei and scant cytoplasm together with peripheral palisading and forming solid nests. Furthermore, the most tumors were composed of highly cellular areas with a homogenous population of round, ovoid and spindle cells, hyperchromatic nuclei, high cellular pleomorphism, high mitotic index and various morphologic patterns. Moreover, the tumors displayed an invasive growth, with positive expression of Cytokeratin 19 (CK19) and negative expression of CK15. Our study revealed that the expression of CK19 was associated with progression and invasion in cases with BCC and immunohistochemistry is indispensable in distinguishing this tumor from other types of cutaneous carcinoma. To our best knowledge, it may be a considerable biomarker to assess invasiveness of cutaneous-surface BCC and to guide clinical management of such tumors.
Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
Katayoun
K
Ziari
Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Behnoosh
B
Behdad
Department of Emergency Medicine, AJA University of Medical Sciences, Tehran, Iran.
Ali
A
Mohammadshahi
Department of Nephrology, AJA University of Medical Sciences, Tehran, Iran.
Hamid Reza
HR
Pirasteh
Department of Emergency Medicine, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad Davood
MD
Sharifi
Department of Pathology, Imam Reza Hospital, AJA University of Medical Sciences, Tehran, Iran. Electronic address: mdadpay@yahoo.com.
Masoomeh
M
Dadpay
D000818:Animals; D002280:Basal Cell Carcinoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry / Q000379:methods; D053569:Keratin-15 / Q000096:biosynthesis*; D053539:Keratin-19 / Q000096:biosynthesis*; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary
D053569:Keratin-15; D053539:Keratin-19; D009363:Neoplasm Proteins
Basal cell carcinoma; Cutaneous; Cytokeratin; Histopathology; Immunohistochemistry