Immune signaling pathways activated in response to different pathogenic micro-organisms in Bombyx mori.
The JAK/STAT, Toll, Imd, and RNAi pathways are the major signaling pathways associated with insect innate immunity. To explore the different immune signaling pathways triggered in response to pathogenic micro-organism infections in the silkworm, Bombyx mori, the expression levels of the signal transducer and activator of transcription (BmSTAT), spatzle-1 (Bmspz-1), peptidoglycan-recognition protein LB (BmPGRP-LB), peptidoglycan-recognition protein LE (BmPGRP-LE), argonaute 2 (Bmago2), and dicer-2 (Bmdcr2) genes after challenge with Escherichia coli (E. coli), Serratiamarcescens (Sm), Bacillus bombyseptieus (Bab), Beauveriabassiana (Beb), nucleopolyhedrovirus (BmNPV), cypovirus (BmCPV), bidensovirus (BmBDV), or Nosemabombycis (Nb) were determined using real-time PCR. We found that the JAK/STAT pathway could be activated by challenge with BmNPV and BmBDV, the Toll pathway could be most robustly induced by challenge with Beb, the Imd pathway was mainly activated in response to infection by E. coli and Sm, and the RNAi pathway was not activated by viral infection, but could be triggered by some bacterial infections. These findings yield insights into the immune signaling pathways activated in response to different pathogenic micro-organisms in the silkworm.
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Wei
W
Liu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Jiabin
J
Liu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Yahong
Y
Lu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Yongchang
Y
Gong
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Min
M
Zhu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Fei
F
Chen
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Zi
Z
Liang
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Liyuan
L
Zhu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
Sulan
S
Kuang
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
Xiaolong
X
Hu
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
Guangli
G
Cao
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
Renyu
R
Xue
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China. Electronic address: gongcl@suda.edu.cn.
Chengliang
C
Gong
D000818:Animals; D001419:Bacteria / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology*; D012831:Bombyx / Q000276:immunology / Q000382:microbiology / Q000821:virology; D019476:Insect Proteins / Q000276:immunology*; D007304:Insect Viruses / Q000276:immunology; D016816:Microsporida / Q000276:immunology; D016881:Microsporidiosis / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D014777:Virus Diseases / Q000276:immunology*
D019476:Insect Proteins
Imd pathway; Innate immunity; JAK/STAT pathway; RNAi pathway; Silkworm; Toll pathway