α-Glucosidase inhibitory activities of fatty acids purified from the internal organ of sea cucumber Stichopus japonicas.
α-Glucosidase inhibitory activities of the various solvent fractions (n-hexane, CHCl3 , EtOAc, BuOH, and water) of sea cucumber internal organ were investigated. 1,3-Dipalmitolein (1) and cis-9-octadecenoic acid (2) with potent α-glucosidase inhibitory activity were purified from the n-hexane fraction of sea cucumber internal organ. IC50 values of compounds 1 and 2 were 4.45 and 14.87 μM against Saccharomyces cerevisiae α-glucosidase. These compounds mildly inhibited rat-intestinal α-glucosidase. In addition, both compounds showed a mixed competitive inhibition against S. cerevisiae α-glucosidase and were very stable at pH 2 up to 60 min. The KI values of compounds 1 and 2 were 0.48 and 1.24 μM, respectively. Therefore, the internal organ of sea cucumber might be a potential new source of α-glucosidase inhibitors suitably used for prevention of obesity and diabetes mellitus.
Dept. of Marine Food Science and Technology, Gangneung-Wonju Natl. Univ, 7 Jukheon-gil, Gangneung, 210-702, Republic of Korea.
T H
TH
Nguyen
S M
SM
Kim
D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D019301:Oleic Acid / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology; D047528:Stichopus / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000378:metabolism*
D001688:Biological Products; D005227:Fatty Acids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D019301:Oleic Acid; D000520:alpha-Glucosidases
antidiabetes; fatty acid; internal organ; sea cucumber; α-glucosidase