Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss).
Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a 'scavenging' receptor for inflammatory CC chemokines and plays a central role in the resolution of in vivo inflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulated in vivo by bacterial and parasitic infections, and in vitro by PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; Key Laboratory of Aquaculture and Ecology of Coastal Pool in Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu, 224051, China.
Zhitao
Z
Qi
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; College of Fishery and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Yousheng
Y
Jiang
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
Jason W
JW
Holland
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China.
Pin
P
Nie
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
Christopher J
CJ
Secombes
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK. Electronic address: t.h.wang@abdn.ac.uk.
Tiehui
T
Wang
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology*; D060226:Head Kidney / Q000378:metabolism*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000378:metabolism* / Q000382:microbiology / Q000469:parasitology; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D011070:Poly I-C / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D054447:Receptors, CCR10 / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000378:metabolism*; D026801:Synteny; D000097628:Chemokine Receptor D6
D016207:Cytokines; D017931:DNA Primers; D010457:Peptidoglycan; D011070:Poly I-C; D054447:Receptors, CCR10; D000097628:Chemokine Receptor D6
Atypical chemokine receptor-2 (ACKR2); CC chemokine binding protein-2 (CCBP2); Expression; Infection; Rainbow trout Oncorhynchus mykiss