[Prevalence and molecular characterization of Giardia lamblia isolates from goats in Anhui Province].
Five hundred and six fresh fecal samples were collected from Lu'an, Fuyang, Suzhou, Chizhou, Wuhu, Chuzhou and Bozhou in Anhui Province, and detected firstly by direct smear microscopy. The microscopy-positive samples were amplified by nested PCR targeting the triosephosphate isomerase (TPI) and glutamate dehydrogenase(GDH) genes. The positive PCR products were sequenced in both directions. The sequences were analyzed by ClustalX 1.81 for sequence alignment and the neighbor-joining trees were constructed by Mega 5.05. Thirty-two out of 506 fecal specimens were diagnosed as Giardia-positive by microscopy with an infection rate of 6.3%. 23 and 16 of the samples were typed as assemblage E by the TPI (530 bp) and GDH (450 bp) genes, respectively. These findings indicated that there was a different distribution of subtypes of assemblage E in different areas. The zoonosis genotypes such as assemblage A or B was not found in the present study.
You-fang
YF
Gu
Li-ke
LK
Wang
Yang
Y
Li
Lei
L
Li
Xing-hai
XH
Chu
Di-wei
DW
Xin
Cheng-xiang
CX
Ma
Wen-han
WH
Xu
Sheng-bing
SB
Wu
He-yin
HY
Wang
Wen-chao
WC
Li
D000818:Animals; D001483:Base Sequence; D005243:Feces; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016415:Sequence Alignment; D014305:Triose-Phosphate Isomerase; D015047:Zoonoses
D005969:Glutamate Dehydrogenase; D014305:Triose-Phosphate Isomerase