Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.).
Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Faculty of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Liying
L
Yu
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Faculty of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Weiqi
W
Tang
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Weiyi
W
He
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Xiaoli
X
Ma
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.
Liette
L
Vasseur
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] School of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Simon W
SW
Baxter
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Guang
G
Yang
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Shiguo
S
Huang
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Faculty of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Fengqin
F
Song
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
Minsheng
M
You
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D008658:Inactivation, Metabolic / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000633:toxicity; D007814:Larva / Q000201:enzymology; D007915:Lepidoptera / Q000201:enzymology*; D005810:Multigene Family; D010802:Phylogeny; D011679:Pupa / Q000201:enzymology
D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System