Sublethal heavy metal stress stimulates innate immunity in tomato.
Effect of sublethal heavy metal stress as plant biotic elicitor for triggering innate immunity in tomato plant was investigated. Copper in in vivo condition induced accumulation of defense enzymes like peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and β-1,3 glucanase along with higher accumulation of total phenol, antioxidative enzymes (catalase and ascorbate peroxidase), and total chlorophyll content. Furthermore, the treatment also induced nitric oxide (NO) production which was confirmed by realtime visualization of NO burst using a fluorescent probe 4,5-diaminofluorescein diacetate (DAF-2DA) and spectrophotometric analysis. The result suggested that the sublethal dose of heavy metal can induce an array of plant defense responses that lead to the improvement of innate immunity in plants.
Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India.
Nilanjan
0000-0001-7351-3200
N
Chakraborty
Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India.
Swarnendu
S
Chandra
Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India.
Krishnendu
K
Acharya
D000704:Analysis of Variance; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004156:Catechol Oxidase / Q000276:immunology; D002734:Chlorophyll / Q000378:metabolism; D005453:Fluorescence; D043326:Glucan 1,3-beta-Glucosidase / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000276:immunology*; D019216:Metals, Heavy / Q000633:toxicity*; D009195:Peroxidase / Q000276:immunology; D010636:Phenols / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000276:immunology; D013053:Spectrophotometry
D019216:Metals, Heavy; D010636:Phenols; D002734:Chlorophyll; D004156:Catechol Oxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D043326:Glucan 1,3-beta-Glucosidase; D010650:Phenylalanine Ammonia-Lyase