Isolation and characterization of microsatellite loci for Cunninghamia lanceolata (Lamb.) Hook.
As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52 individuals from the Provenance Test Plantation in Fenyi, Jiangxi Province, China. Among the loci, 10 were polymorphic and 1-34 (average 18.182) alleles per locus were identified. Observed and expected heterozygosities ranged from 0 to 0.750 (mean 0.456) and 0 to 0.968 (mean 0.749), respectively. These microsatellite loci may facilitate further research on the molecular breeding and population genetics of C. lanceolata and its relatives.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Y X
YX
Li
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Z S
ZS
Wang
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
J K
JK
Sui
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Y F
YF
Zeng
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China duanag@163.com.
A G
AG
Duan
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
J G
JG
Zhang
D032486:Cunninghamia / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D056426:Genetic Loci; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data
D018744:DNA, Plant