Expression of intercellular adhesion molecule-1 by myofibers in mdx mice.
BACKGROUND
We investigated the extent to which intercellular adhesion molecule-1 (ICAM-1), a critical protein of the inflammatory response, is expressed in skeletal muscles of mdx mice (a murine model of Duchenne muscular dystrophy).


METHODS
Muscles were collected from control and mdx mice at 2-24 weeks of age and analyzed for ICAM-1 expression by means of Western blot and immunofluorescence.


RESULTS
Western blot revealed higher expression of ICAM-1 in mdx compared with control muscles through 24 weeks of age. In contrast to control muscles, ICAM-1 was expressed on the membrane of damaged, regenerating, and normal myofibers of mdx mice. CD11b+ myeloid cells also expressed ICAM-1 in mdx muscles, and CD11b+ cells were closely associated with the membrane of myofibers expressing ICAM-1.


CONCLUSIONS
These findings support a paradigm in which ICAM-1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology.
Department of Kinesiology, The University of Toledo, 2801 W. Bancroft Street, Toledo, Ohio, 43606, USA.
Maria J
MJ
Torres-Palsa
Department of Kinesiology, The University of Toledo, 2801 W. Bancroft Street, Toledo, Ohio, 43606, USA.
Matthew V
MV
Koziol
Department of Kinesiology, The University of Toledo, 2801 W. Bancroft Street, Toledo, Ohio, 43606, USA.
Qingnian
Q
Goh
Department of Kinesiology, The University of Toledo, 2801 W. Bancroft Street, Toledo, Ohio, 43606, USA.
Peter A
PA
Cicinelli
Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, Columbus, Ohio, USA.
Jennifer M
JM
Peterson
Department of Kinesiology, The University of Toledo, 2801 W. Bancroft Street, Toledo, Ohio, 43606, USA.
Francis X
FX
Pizza
D000818:Animals; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis / Q000378:metabolism*
D018799:Intercellular Adhesion Molecule-1
Duchenne muscular dystrophy; macrophages; muscle inflammation; neutrophils; β2 integrins