Evaluation of a new technique for preparation of endothelial grafts for descemet membrane endothelial keratoplasty.
OBJECTIVE
The purpose of this study was to compare the Muraine technique, a relatively new method for preparing endothelial grafts for Descemet membrane endothelial keratoplasty (DMEK), with the current standard submerged cornea using backgrounds away (SCUBA) peeling technique.


METHODS
This study was a prospective ex vivo investigation. In a wet-lab setting, 20 donor corneas were prepared for DMEK using The Muraine technique and 20 donor corneas using the SCUBA technique. In each of the technique groups, 10 corneas were prepared by a corneal surgeon and 10 were prepared by a corneal fellow. Primary outcome measures were the time needed to prepare endothelial grafts and the number of graft tears.


RESULTS
In the SCUBA technique, median time to prepare grafts was shorter for both the surgeon (301 ± 85 seconds) and fellow (523 ± 58 seconds) compared with the Muraine technique (surgeon, 359 ± 83 seconds; fellow, 543 ± 44 seconds). However, these findings were not statistically significant (surgeon, P = 0.33; fellow, P = 0.24; pooled, P = 0.46). There was a statistically significant difference between surgeon time and fellow time for each technique (SCUBA technique, P = 0.0005; Muraine technique, P = 0.002). In the Muraine technique, there were 5 graft tears (surgeon = 2, fellow = 3), and no graft tears in the SCUBA technique, which was statistically significant (P = 0.047).


CONCLUSIONS
The present study demonstrates that the SCUBA technique may be a more effective technique to prepare endothelial donor grafts for DMEK.
*Department of Ophthalmology, Queen's University, Kingston, ON, Canada; and †University of Ottawa Eye Institute, Ottawa, ON, Canada.
Ashley
A
Brissette
Ronan
R
Conlon
Joshua C
JC
Teichman
Season
S
Yeung
Setareh
S
Ziai
Kashif
K
Baig
D002452:Cell Count; D057111:Descemet Stripping Endothelial Keratoplasty; D004728:Endothelium, Corneal / Q000637:transplantation*; D006801:Humans; D011446:Prospective Studies; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods*