MhNCED3, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Malus hupehensis Rehd., enhances plant tolerance to Cl- stress by reducing Cl- accumulation.
High Cl(-) concentrations in tissues can be toxic to crop plants and may lead to reduced growth rates and yields. 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be involved in the biosynthesis of abscisic acid (ABA), which is an important regulator of plant adaptive responses to stress. Here, the expression of MhNCED3 in Malus hupehensis Rehd. and the effects of MhNCED3 on plant tolerance to Cl(-) stress were explored. The results showed that MhNCED3 expression and ABA biosynthesis in M. hupehensis Rehd. were induced by Cl(-) stress. Ectopic expression of MhNCED3 in Arabidopsis complemented the phenotypic defects of the 129B08/nced3 mutant and enhanced WT tolerance to Cl(-) stress. The transgenic Arabidopsis showed improved growth and developmental status, increased ABA contents, and reduced transpiration rates and relative water content. Furthermore, ectopic expression of MhNCED3 decreased Cl(-) accumulation and oxidative damage, and up-regulated the expression levels of AtCLCc (chloride channel protein) and AtSLAH3 (slow anion channel 1 homolog 3) genes in Arabidopsis. These observations suggest that MhNCED3 has critical role in enhancing plant tolerance to Cl(-) stress by reducing Cl(-) accumulation.
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
Wei-wei
WW
Zhang
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China. Electronic address: hqyang@sdau.edu.cn.
Hong-qiang
HQ
Yang
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
Shu-zhen
SZ
You
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
Shu-lei
SL
Fan
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong 271018, PR China.
Kun
K
Ran
D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D002712:Chlorides / Q000009:adverse effects / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D027845:Malus / Q000201:enzymology / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018526:Plant Transpiration / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D015854:Up-Regulation; D014867:Water / Q000378:metabolism
D029681:Arabidopsis Proteins; D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins; D010940:Plant Proteins; C528438:SLAC1 protein, Arabidopsis; D014867:Water; D000040:Abscisic Acid; D049308:Dioxygenases; C109043:9-cis-epoxy-carotenoid dioxygenase
Cl(–) accumulation; Ectopic expression; Malus hupehensis Rehd.; NCED; Tolerance