Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel.
Three-dimensional (3D) cell manipulation is available with the integration of microfluidic technology and rapid prototyping techniques. High-Fidelity (Hi-Fi) constructs hold enormous therapeutic potential for organ manufacturing and regenerative medicine. In the present paper we introduced a quasi-three-dimensional (Q3D) model with parallel biocompatible alginate/gelatin/fibrin hurdles. The behaviors of fluids and cells along the microfluidic channels with various widths were studied. Cells inside the newly designed microfluidic channels attached and grew well. Morphological changes of adipose-derived stem cells (ADSCs) in both two-dimensional (2D) and 3D milieu were found on the printed constructs. Endothelialization occurred with the co-cultures of ADSCs and hepatocytes. This study provides insights into the interactions among fluids, cells and biomaterials, the behaviors of fluids and cells along the microfluidic channels, and the applications of Q3D techniques.
Department of Mechanical Engineering, Key Laboratory for Advanced Materials Processing Technology, Ministry of Education & Center of Organ Manufacturing, Tsinghua University, Beijing, 100084, P.R. China.
Yufan
Y
Xu
Department of Mechanical Engineering, Key Laboratory for Advanced Materials Processing Technology, Ministry of Education & Center of Organ Manufacturing, Tsinghua University, Beijing, 100084, P.R. China. wangxiaohong@mail.tsinghua.edu.cn.|State Key Laboratory of Materials Processing and Die & Mould Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R. China. wangxiaohong@mail.tsinghua.edu.cn.
Xiaohong
X
Wang
D000464:Alginates; D002454:Cell Differentiation; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000502:physiology*; D005337:Fibrin; D005780:Gelatin; D020723:Glucuronic Acid; D022781:Hepatocytes / Q000502:physiology*; D006603:Hexuronic Acids; D044085:Microfluidics / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin; D005337:Fibrin
3D printing; adipose-derived stem cells (ADSCs); hepatocytes; microfluidics; parallel alginate/gelatin/fibrin channels