MicroRNA-575 targets BLID to promote growth and invasion of non-small cell lung cancer cells.
This study was designed to detect miR-575 expression and function in non-small cell lung cancer (NSCLC). A higher expression of miR-575 in NSCLC tissues was observed compared with adjacent non-neoplastic tissues. Furthermore, re-introduction of miR-575 significantly promoted cell proliferation, migration, and invasion in the NSCLC line. Moreover, we showed that BLID is negatively regulated by miR-575 at the posttranscriptional level, via a specific target site within the 3'UTR. Overexpression of BLID counteracted miR-575-induced proliferation and invasion in NSCLC cells. The expression of BLID is frequently downregulated in NSCLC tumors and cell lines and inversely correlates with miR-575 expression. The findings of this study contribute to the current understanding of the functions of miR-575 in NSCLC.
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Hongwei
H
Wang
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Chunhua
C
Yan
Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Xiaodong
X
Shi
Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Jiaolin
J
Zheng
Department of Oncology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Lili
L
Deng
Department of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Lei
L
Yang
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Fangfei
F
Yu
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
Yuandi
Y
Yang
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China. Electronic address: shaoyuxiadoctor@163.com.
Yuxia
Y
Shao
D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness
D051017:Apoptosis Regulatory Proteins; C118024:BLID protein, human; C000597892:MIRN575 microRNA, human; D035683:MicroRNAs
BLID; MicroRNA-575; Non-small cell lung cancer; Oncogene