Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells.
The purpose of this study was to investigate the influence of superhydrophilic treatments of titanium on the behavior of osteoblastlike cells. Superhydrophilic specimens were prepared with sandblast and acid-etching (DW), oxygen plasma (Plasma) and ultraviolet light (UV), and were stored in distilled water for 3 days immediately after these treatments. Specimens stored in air for 3 weeks were used as a control Air group. Initial cell attachment, proliferation, alkaline phosphatase activity, and osteocalcin secretion of mouse osteoblast-like cells MC3T3-E1 were enhanced more on superhydrophilic groups than were Air specimens. On confocal laser scanning microscope images of cell morphology, the expression of actin filaments was observed on the superhydrophilic groups, whereas relatively little actin filament expression was seen on the Air surfaces on all culture periods. These results indicate that DW, Plasma, or UV treatment has potential for the creation and maintenance of superhydrophilic surfaces and the enhancement of the initial attachment, proliferation, and differentiation of osteoblast-like cells.
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502; Department of Endodontics and Clinical Cariology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku 101-0061, Japan.
Keisuke
K
Yamamura
Tadashi
T
Miura
I
I
Kou
Takashi
T
Muramatsu
Masahiro
M
Furusawa
Masao
M
Yoshinari
D000134:Acid Etching, Dental; D000199:Actins / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D057927:Hydrophobic and Hydrophilic Interactions; D066298:In Vitro Techniques; D051379:Mice; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*
D000199:Actins; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase