Osteopontin-induced brown adipogenesis from white preadipocytes through a PI3K-AKT dependent signaling.
Recent studies have shown that OPN (osteopontin) plays critical roles in cell survival, differentiation, bio-mineralization, cancer and cardiovascular remodeling. However, its roles in the differentiation of brown adipocytes and the underlying mechanisms remain unclear. Therefore, the aim of this study was to investigate the roles of OPN in the brown adipogenesis and the underlying mechanisms. It was shown that the OPN successfully induced the differentiation of 3T3-L1 white preadipocytes into the PRDM16(+) (PRD1-BF1-RIZ1 homologous domain containing 16) and UCP-1(+) (uncoupling protein-1) brown adipocytes in a concentration and time-dependent manner. Also, activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for the OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of the brown adipocytes and might provide a potential novel therapeutic approach for the treatment of obesity and related disorders.
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Xiao-Juan
XJ
Zhong
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Xiao-Dan
XD
Shen
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Jian-Bing
JB
Wen
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Ying
Y
Kong
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Jia-Jia
JJ
Chu
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Guo-Qiang
GQ
Yan
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Teng
T
Li
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Dan
D
Liu
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Meng-Qing
MQ
Wu
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Guo-Hua
GH
Zeng
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Ming
M
He
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China. Electronic address: qrhuang@ncu.edu.cn.
Qi-Ren
QR
Huang
D041721:3T3-L1 Cells; D052438:Adipocytes, White / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D039302:Integrin alphaVbeta3; D011994:Recombinant Proteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
Adipocyte; Differentiation; Obesity; Osteopontin; PRDM16