Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae).
Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest.
Univ Estadual Paulista "Júlio de Mesquita Filho" UNESP/FCAV, Department of Applied Biology, Rod Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, São Paulo, Brazil; Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Camila C
CC
Davolos
Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Patricia
P
Hernández-Martinez
Univ Estadual Paulista "Júlio de Mesquita Filho" UNESP/FCAV, Department of Applied Biology, Rod Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Paula C B
PC
Crialesi-Legori
Univ Estadual Paulista "Júlio de Mesquita Filho" UNESP/FCAV, Department of Applied Biology, Rod Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Janete A
JA
Desidério
Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Juan
J
Ferré
Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Baltasar
B
Escriche
Univ Estadual Paulista "Júlio de Mesquita Filho" UNESP/FCAV, Department of Applied Biology, Rod Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, São Paulo, Brazil. Electronic address: mvictor@fcav.unesp.br.
Manoel Victor F
MV
Lemos
D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007305:Insecticide Resistance / Q000502:physiology*; D009036:Moths / Q000378:metabolism*; D010572:Pest Control, Biological / Q000379:methods*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Binding site models; Insect control; Insect corn pest; Insect resistance management; Insecticidal proteins