A simple microviscometric approach based on Brownian motion tracking.
Viscosity-an integral property of a liquid-is traditionally determined by mechanical instruments. The most pronounced disadvantage of such an approach is the requirement of a large sample volume, which poses a serious obstacle, particularly in biology and biophysics when working with limited samples. Scaling down the required volume by means of microviscometry based on tracking the Brownian motion of particles can provide a reasonable alternative. In this paper, we report a simple microviscometric approach which can be conducted with common laboratory equipment. The core of this approach consists in a freely available standalone script to process particle trajectory data based on a Newtonian model. In our study, this setup allowed the sample to be scaled down to 10 μl. The utility of the approach was demonstrated using model solutions of glycerine, hyaluronate, and mouse blood plasma. Therefore, this microviscometric approach based on a newly developed freely available script can be suggested for determination of the viscosity of small biological samples (e.g., body fluids).
Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic.
Zuzana
Z
Hnyluchová
Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic.
Petra
P
Bjalončíková
Faculty of Informatics, Masaryk University in Brno, Botanická 68A, 602 00 Brno, Czech Republic.
Pavel
P
Karas
Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic.
Filip
F
Mravec
Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic.
Tereza
T
Halasová
Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic.
Miloslav
M
Pekař
International Clinical Research Center-Center of Biomolecular and Cell Engineering, St. Anne's University Hospital Brno, Pekařská 53, 656 91 Brno, Czech Republic.
Lukáš
L
Kubala
International Clinical Research Center-Center of Biomolecular and Cell Engineering, St. Anne's University Hospital Brno, Pekařská 53, 656 91 Brno, Czech Republic.
Jan
J
Víteček
D000818:Animals; D001809:Blood Viscosity; D006820:Hyaluronic Acid / Q000737:chemistry; D051379:Mice; D055616:Microtechnology / Q000295:instrumentation*; D009038:Motion; D014783:Viscosity
D006820:Hyaluronic Acid