[Protective effect of total glycosides of Ranunculus japonicus on myocardial ischemic-reperfusion injury in isolated rat hearts].
OBJECTIVE
To study the protective effect of total glycosides of Ranunculus japonicus (TGRJ) on myocardial ischemic-reperfusion injury in isolated rat hearts.


METHODS
The SD rats were randomly divided into normal control group, ischemia-reperfusion group, and TGRJ in 0.05, 0.10 and 0.20 mg/mL groups. The ischemia-reperfusion injury model was built using Langendroff isolated rats hearts perfusion system. The indexes of heart function such as heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular systolic pressure maximum rate of rise (+ dp/dt(max)), left ventricular diastolic pressure maximum rate of descent (-dp/dt(max)) and coronary flow (CF) before ischemia and later at 1, 5, 10, 20, 30 and 40 min after reperfusion were recorded. The activity of Lactate dehydrogenase (LDH) and creatine kinase (CK) at 20 and 40 min after reperfusion were determined. The myocardial tissues were stained with Triphenyltetrazolium chloride (TTC) and the percentage of myocardial infarction area was calculated.


RESULTS
HR, LVDP, +dp/dt(max) and CF of the I/R group were significantly decreased after reperfusion, LDH and CK levels were increased and the area of myocardial infraction was 58.78%. TGRJ had improving effect on all above indexes.


CONCLUSIONS
TGRJ has protective effet on the myo- cardial ischemic-reperfusion injury.
Xiao-Wei
XW
Gao
Yuan
Y
Liu
Zhi-Cheng
ZC
Yang
Yu-Zhi
YZ
Tan
D000818:Animals; D001794:Blood Pressure; D003402:Creatine Kinase; D006027:Glycosides / Q000494:pharmacology*; D006339:Heart Rate; D007770:L-Lactate Dehydrogenase; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D031947:Ranunculus / Q000737:chemistry*; D051381:Rats
D006027:Glycosides; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase