[Clinical features and gene mutation profiles of patients with chronic hepatitis B and Gilbert's syndrome].
OBJECTIVE
To explore the clinical features and gene mutation profiles of patients with chronic hepatitis B (CHB) and Gilbert's syndrome.


METHODS
Thirty-three patients with CHB and Gilbert's syndrome were enrolled in the study. Serum markers of liver function and histological features of disease-related liver injury were assessed by standard methods. Gene mutations were detected by PCR and direct DNA sequencing.Statistical analysis was carried out with the chi-square and t tests.


RESULTS
Sequencing of the Gilbert syndrome-associated gene, UGT 1A 1, revealed mutations in the upstream promoter phenobarbital-responsive element module (PBREM) (-3279 mutation, 23 cases), in the promoter TATA box (a TA insertion mutation, 21 cases), and in the coding region of exon 1 (a GGA-AGA Gly71Arg mutation, 18 cases); there was no statistical difference found for any of the three mutations among this patient population (x2 =1.640, P more than 0.05).


CONCLUSIONS
The traditional methods of diagnosis for patients with CHB and Gilbert's syndrome remain a technical challenge in the clinic, and gene detection may represent a more favorable method for diagnosing this patient population.
Department of Infectious Diseases, Henan Provincal People's Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, China.
Huibin
H
Ning
Kuan
K
Li
Zhongshan
Z
Mao
Junping
J
Liu
Erhui
E
Xiao
Yi
Y
Kang
Jia
J
Shang
D001483:Base Sequence; D005091:Exons; D005878:Gilbert Disease; D014453:Glucuronosyltransferase; D019694:Hepatitis B, Chronic; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016385:TATA Box
D014453:Glucuronosyltransferase