Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus.
PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.
Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: felsberg@biomed.cas.cz.
Jurgen
J
Felsberg
Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: marketaj@biomed.cas.cz.
Markéta
M
Jelínková
Department of Microbiology, Research Institute of Brewing and Malting, Lípová 15, 120 44 Prague, Czech Republic. Electronic address: kubizniakova@beerresearch.cz.
Petra
P
Kubizniaková
Department of Microbiology, Research Institute of Brewing and Malting, Lípová 15, 120 44 Prague, Czech Republic. Electronic address: matoulkova@beerresearch.cz.
Dagmar
D
Matoulková
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
D021901:DNA, Intergenic; D012335:RNA, Ribosomal
Brewing microbiology; PCR; Strictly anaerobic bacteria; Yeast contamination; Zymophilus paucivorans; Zymophilus raffinosivorans