A novel alkaline hemicellulosic heteroxylan isolated from alfalfa (Medicago sativa L.) stem and its thermal and anti-inflammatory properties.
A novel hemicellulosic polysaccharide (ACAP) was purified from the cold alkali extraction of alfalfa stems and characterized as a heteroxylan with a weight-average molecular weight of 7.94 × 10(3) kDa and a radius of 58 nm. Structural analysis indicated that ACAP consisted of a 1,4-linked β-D-Xylp backbone with 4-O-MeGlcpA and T-L-Araf substitutions at O-2 and O-3 positions, respectively. Transmission electron microscopy (TEM) examination revealed the entangled chain morphology of ACAP molecules. The evaluation of thermal degradation property revealed a primary decomposition temperature range of 238.8-314.0 °C with an apparent activation energy (Ea) and a pre-exponential factor (A) of 220.0 kJ/mol and 2.81 × 10(24)/s, respectively. ACAP also showed significant inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in cultured RAW 264.7 mouse macrophage cells. These results suggested the potential utilization of ACAP in functional foods and dietary supplement products.
Lei
L
Chen
Jie
J
Liu
Yaqiong
Y
Zhang
Yuge
Y
Niu
Bona
B
Dai
§Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742, United States.
Liangli Lucy
LL
Yu
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004355:Drug Stability; D006358:Hot Temperature; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D000455:Medicago sativa / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D010936:Plant Extracts; D011134:Polysaccharides; C007916:hemicellulose
alfalfa polysaccharide; anti-inflammatory effect; hemicellulosic heteroxylan; structural analysis; thermal decomposition