Virgin olive oil rich in phenolic compounds modulates the expression of atherosclerosis-related genes in vascular endothelium.
OBJECTIVE
Previous studies have shown the anti-inflammatory and antioxidant properties of phenolic compounds of virgin olive oil (VOO). However, the effect of bioavailable phenolic compounds on the vascular endothelium is unknown. We aimed to evaluate the effect of the consumption of virgin olive oil rich in phenolic compounds on the vascular endothelium.


METHODS
We treated HUVEC with human serum obtained in fasting state and after the intake of a breakfast prepared with VOO with a high or low content of phenolic compounds.


RESULTS
Treatment of HUVEC with serum obtained 2 h after the intake of the high-phenol VOO-based breakfast decreased p65 and MCP-1 gene expression (p < 0.001 and p = 0.002, respectively) and increased MT-CYB, SDHA and SOD1 gene expression (p = 0.004, p = 0.012 and p = 0.001, respectively), as compared with the treatment of HUVEC with the serum obtained 2 h after the intake of the low-phenol VOO-based breakfast. The treatment with serum obtained 4 h after the intake of the high-phenol VOO-based breakfast decreased MCP-1 and CAT gene expression (p < 0.001 and p = 0.003, respectively) and increased MT-CYB gene expression (p < 0.001), as compared to the treatment with serum obtained 4 h after the intake of the low-phenol VOO-based breakfast.


CONCLUSIONS
Our results suggest that the consumption of virgin olive oil rich in phenolic compounds may reduce the risk of atherosclerosis development by decreasing inflammation and improving the antioxidant profile in the vascular endothelium.
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Eliana R
ER
Meza-Miranda
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Oriol A
OA
Rangel-Zúñiga
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Carmen
C
Marín
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Pablo
P
Pérez-Martínez
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Javier
J
Delgado-Lista
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Carmen
C
Haro
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Patricia
P
Peña-Orihuela
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Ana I
AI
Jiménez-Morales
CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.|Department of Cell Biology, Physiology, and Immunology, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Cordoba, Spain.
María M
MM
Malagón
CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.|Endocrinology and Nutrition Service, Hospital Virgen de la Victoria, Malaga, Spain.
Francisco J
FJ
Tinahones
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
José
J
López-Miranda
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Francisco
F
Pérez-Jiménez
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Av. Menendez Pidal, s/n., 14004, Cordoba, Spain. antonio.camargo@imibic.org.|CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. antonio.camargo@imibic.org.
Antonio
A
Camargo
D050197:Atherosclerosis / Q000235:genetics* / Q000517:prevention & control*; D001682:Biological Availability; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D042963:Electron Transport Complex II / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D005215:Fasting; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000069463:Olive Oil / Q000008:administration & dosage* / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000493:pharmacokinetics; D000072105:Superoxide Dismutase-1 / Q000235:genetics / Q000378:metabolism
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D000069463:Olive Oil; D010636:Phenols; C000606290:SOD1 protein, human; C577219:CAT protein, human; D002374:Catalase; D000072105:Superoxide Dismutase-1; D042963:Electron Transport Complex II; C511268:SDHA protein, human
Gene expression; HUVEC; Phenolic compounds; Vascular endothelium; Virgin olive oil