Extracts of Leptadenia hastata Leaf, a Famine Food and Traditional Remedy for Furuncles, Suppress Inflammation in Murine Models.
The effects of the methanol extract (LHE), hexane (LHHF), ethylacetate (LHEF) and methanol (LHMF) fractions of leaf of Leptadenia hastata on acute and chronic inflammation were studied. Furthermore, the effects of LHE on acetic acid induced increase in vascular permeability, carrageenan induced leucocyte migration and membrane stability were evaluated. The LHE and fractions were also subjected to phytochemical analysis. The LHE, LHEF and LHMF significantly (p < 0.05) suppressed topical ear edema, systemic paw edema, global edematous response to formaldehyde arthritis and granuloma tissue growth. The LHE suppressed acetic acid induced vascular permeability and carrageenan-induced leucocyte migration, and also stabilized the erythrocyte membrane. An acute toxicity test in mice established an oral LD50 > 5 g/kg for LHE. The LHEF elicited the greatest inhibition, suggesting that the observed anti-inflammatory effects may be attributable to the flavonoids abundant in the fraction. These findings demonstrate that the effectiveness of L. hastata leaf in the treatment of furuncles may largely derive from anti-inflammatory activities mediated through inhibition of both increase in vascular permeability and leucocyte migration, and stabilization of cell membranes.
a 1 Department of Pharmacology & Toxicology.
Adaobi Chioma
AC
Ezike
a 1 Department of Pharmacology & Toxicology.
Ifechukwu Kenneth
IK
Ufere
a 1 Department of Pharmacology & Toxicology.
Peter Achunike
PA
Akah
b 2 Department of Pharmacognosy & Environmental Medicines, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
Samson Chukwuemeka
SC
Ezea
a 1 Department of Pharmacology & Toxicology.
Charles Ogbonnaya
CO
Okoli
D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D001168:Arthritis / Q000139:chemically induced / Q000188:drug therapy; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan / Q000008:administration & dosage / Q000633:toxicity; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005557:Formaldehyde; D005667:Furunculosis; D006099:Granuloma / Q000139:chemically induced / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy*; D007928:Lethal Dose 50; D051379:Mice; D064209:Phytochemicals / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D023382:Toxicity Tests, Acute
D000893:Anti-Inflammatory Agents; D064209:Phytochemicals; D010936:Plant Extracts; D005557:Formaldehyde; D002351:Carrageenan; D019342:Acetic Acid
Leptadenia hastata; abscess; anti-inflammatory; furuncles; leucocyte migration; membrane stabilization; vascular permeability