Detection of DNA utilizing a fluorescent reversible change of a biosensor based on the electron transfer from quantum dots to polymyxin B sulfate.
A fluorescent "turn off-on" pattern for the detection of herring sperm DNA (hsDNA) had been designed through utilizing the interaction between polymyxin B sulfate (PMBS) and hsDNA as an inherent performance and the fluorescent transformation of glutathione (GSH)-capped CdTe quantum dots (QDs) as an external manifestation. Due to the occurrence of the photoinduced electron transfer from the QDs to PMBS, the fluorescence of GSH-capped CdTe QDs could be effectively quenched by PMBS, causing the system into "off" state. With the addition of hsDNA, the quenched fluorescence of GSH-capped CdTe QDs could be restored for the reason that PMBS embedded into hsDNA double helix structure to form new complex and peeled off from the surface of GSH-capped CdTe QDs, leading the system into "on" condition. Corresponding experimental results illustrated that the relative recovered fluorescence intensity of GSH-capped CdTe QDs-PMBS system was near proportional to the concentration of hsDNA within the range of 0.059-15.0 μg mL(-1). This proposed method demonstrated a good linear correlation coefficient of 0.9937 and a detection limit (3 σ/K) of 0.018 μg mL(-1) for hsDNA. This dual-directional fluorescent biosensor overcame the selectivity problem commonly existed in the traditional mono-directional fluorescence detection mode and owned perfect analysis applications in biochemical DNA monitoring.
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Linlin
L
Wang
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Shaopu
S
Liu
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Wanjun
W
Liang
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Dan
D
Li
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China; School of Chemical and Environmental Engineering, Chongqing Three Gorges University, Chongqing, Wanzhou 404000, PR China.
Jidong
J
Yang
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: heyq@swu.edu.cn.
Youqiu
Y
He
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D004579:Electron Transport; D004583:Electrons; D005399:Fishes; D005978:Glutathione / Q000737:chemistry*; D057230:Limit of Detection; D011112:Polymyxin B / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D004247:DNA; D005978:Glutathione; D011112:Polymyxin B; D013691:Tellurium; C028337:cadmium telluride
Detection; Fluorescence; Herring sperm DNA; Polymyxin B sulfate; Quantum dots