Biodegradation and utilization of dimethylformamide by biofilm forming Paracoccus sp. strains MKU1 and MKU2.
Two bacterial strains capable of degrading N,N-dimethylformamide (DMF) were isolated from the effluent and sludge samples of textile and tyre industries. The 16S rRNA gene analysis revealed that bacterial strains belonged to the genera Paracoccus and named as Paracoccus sp. MKU1 and Paracoccus sp. MKU2. The DMF degradation experiments conducted at a DMF concentration of 1% v/v and HPLC analysis revealed that MKU1 and MKU2 degraded 55% and 46% of DMF after 120 h of growth. Biofilm quantification by microtiter plate assay revealed that both the bacterial isolates can form efficient biofilm on during DMF utilization. The presence of secondary carbon sources influenced the DMF degradation and biofilm formation where highest biofilm formation was observed in the presence of acetate and enhanced the DMF degradation to a maximum of 86.59% with MKU1 whereas glucose and acetate enhanced DMF degradation by MKU2 to a maximum of 82.7% and 80% respectively.
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Kamaldeen Nasrin
KN
Nisha
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Venkatesan
V
Devi
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Perumal
P
Varalakshmi
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India. Electronic address: rbashokkumar@yahoo.com.
Balasubramaniem
B
Ashokkumar
D000085:Acetates / Q000737:chemistry; D001673:Biodegradation, Environmental; D018441:Biofilms; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004126:Dimethylformamide / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D007700:Kinetics; D009584:Nitrogen / Q000737:chemistry; D010230:Paracoccus / Q000145:classification / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity
D000085:Acetates; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002244:Carbon; D004126:Dimethylformamide; D005947:Glucose; D009584:Nitrogen
Acetate; Biofilm; HPLC; N,N-dimethylformamide; Paracoccus sp.