Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection.
Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting (ta)-siRNAs, most of which target mRNAs of defense-related proteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta-siRNA-mediated silencing on NBS-LRR-disease-resistance proteins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.|Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210095, China.
Liu
L
Yang
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Xiaoying
X
Mu
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Chao
C
Liu
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Jinghui
J
Cai
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Ke
K
Shi
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Wenjiao
W
Zhu
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Qing
Q
Yang
D001483:Base Sequence; D001665:Binding Sites; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D056426:Genetic Loci; D000090263:Leucine-Rich Repeat Proteins; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000096:biosynthesis; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000235:genetics* / Q000382:microbiology*; D020172:Verticillium / Q000502:physiology*
D000090263:Leucine-Rich Repeat Proteins; D035683:MicroRNAs; D011506:Proteins; D034741:RNA, Small Interfering
Verticillium dahliae; Verticillium wilt; miR482; nucleotide binding site leucine-rich repeat; potato; sRNA; ta-siRNA