The role of extracellular inducer of matrix metalloproteinases in premature rupture of membranes.
OBJECTIVE
To investigate the role of matrix metalloproteinases (MMP-2, MMP-9) and their inducer (CD147) in premature rupture of membranes (PROM) at term labor.


METHODS
In a cross-sectional study, 24 women aged 19-39, with 37-40-week pregnancy, and no clinical and histological signs of chorioamnionitis, were divided into two groups with and without PROM. The histological and immunohistochemical study of the fetal membranes was performed with polyclonal rabbit antibodies to MMP-2/MMP-9 and monoclonal rabbit antibodies to CD147.


RESULTS
The analysis of MMP revealed the increase of MMP-9 expression in the amniotic epithelium during premature membrane rupture both in rupture area, and beyond it, and increased MMR-2 expression in the mesodermal cells. We also found high level of CD147 in the amniotic epithelium in PROM group. The above-mentioned changes were found in all areas of fetal membranes, regardless of the rupture localization.


CONCLUSIONS
The study results demonstrate the increased expression of MMR-2 and MMR-9, which regulate the catabolism of fetal membrane extracellular matrix proteins, in amniotic membranes of women with PROM at term labor. The increased expression of CD147 may be one of the mechanisms triggering PROM in the absence of infection.
a Directorate, Federal State Budget Institution, "Research Center for Obstetrics, Gynecology and Perinatology" of Ministry of Healthcare of the Russian Federation , Moscow , Russian Federation .
Gennady T
GT
Sukhikh
b Obstetric Observational Department .
Natalia E
NE
Kan
b Obstetric Observational Department .
Victor L
VL
Tyutyunnik
b Obstetric Observational Department .
Maya V
MV
Sannikova
c Department of Pathology , and.
Elena A
EA
Dubova
c Department of Pathology , and.
Konstantin A
KA
Pavlov
b Obstetric Observational Department .
Elrad Y
EY
Amiraslanov
d R&D Department , Federal State Budget Institution, "Research Center for Obstetrics, Gynecology and Perinatology" of Ministry of Healthcare of the Russian Federation , Moscow , Russian Federation.
Nataliya V
NV
Dolgushina
D000328:Adult; D000650:Amnion / Q000378:metabolism*; D051926:Basigin / Q000378:metabolism; D003430:Cross-Sectional Studies; D042783:Endothelial Cells / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
C497886:BSG protein, human; D051926:Basigin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
CD147; MMP-2; MMP-9; premature rupture of membranes (PROM); spontaneous delivery