Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil.
The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil. Electronic address: sgennari@usp.br.
Solange M
SM
Gennari
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Patrícia de O
Pde O
Esmerini
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Marcos G
MG
Lopes
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Herbert S
HS
Soares
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil; Curso de Medicina Veterinária, FMU, São Paulo, SP, Brazil.
Sérgio N
SN
Vitaliano
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Aline D
AD
Cabral
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Hilda F J
HF
Pena
Colegiado de Medicina Veterinária, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil.
Maurício C
MC
Horta
Instituto de Teologia Aplicada, Faculdades INTA, Sobral, CE, Brazil.
Paulo H
PH
Cavalcante
Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.
Kleber P
KP
Fortes
Instituto Biológico, São Paulo, SP, Brazil.
Eliana M C
EM
Villalobos
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D018397:Equidae; D005838:Genotype; D013045:Species Specificity; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000453:epidemiology / Q000276:immunology*
D000913:Antibodies, Protozoan
Brazil; Equidae; Genotyping; Isolation; Toxoplasma gondii