In situ synthesis of homochiral metal-organic framework in capillary column for capillary electrochromatography enantioseparation.
Homochiral metal-organic frameworks (MOFs) are promising as porous stationary phase for open-tubular capillary electrochromatography (OT-CEC) enantioseparation owing to their fine-tuned pore sizes and large surface areas. In this work, the homochiral MOF AlaZnCl was successfully coated on the inner wall of fused silica capillary by an in situ, layer-by-layer self-assembly approach at room temperature. The results of scanning electron microscopy (SEM), X-ray diffraction (XRD), streaming potential (SP) and Fourier-transform infrared spectroscopy (FT-IR) indicated that the homochiral MOF AlaZnCl was successfully coated on the capillary inner wall. To evaluate the performance of the homochiral MOF AlaZnCl coated capillary column, the enantioseparation was carried out by using six amine drugs and monoamine neurotransmitters as model analytes and excellent enantioseparation efficiency was achieved. Run-to-run, day-to-day, and column-to-column relative standard deviations (RSDs) were all less than 5%. Moreover, the separation efficiency of the homochiral MOF AlaZnCl coated capillary column did not decrease obviously over 100 runs.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Congjie
C
Pan
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Weifeng
W
Wang
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Huige
H
Zhang
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Laifang
L
Xu
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.
Xingguo
X
Chen
D053801:Capillary Electrochromatography / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013237:Stereoisomerism; D014961:X-Ray Diffraction / Q000379:methods*
D009942:Organometallic Compounds; D012822:Silicon Dioxide
Enantioseparation; Homochiral metal–organic framework; In situ synthesis; MOF AlaZnCl; Open tubular capillary electrochromatography