Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry.
Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China. gmhuang@ustc.edu.cn.
Gongyu
G
Li
Jiying
J
Pei
Yue
Y
Yin
Guangming
G
Huang
D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004220:Disulfides; D007328:Insulin; D010455:Peptides