Anti-acetylcholinesterase and Antioxidant Activities of Inhaled Juniper Oil on Amyloid Beta (1-42)-Induced Oxidative Stress in the Rat Hippocampus.
Juniper volatile oil is extracted from Juniperus communis L., of the Cupressaceae family, also known as common juniper. Also, in aromatherapy the juniper volatile oil is used against anxiety, nervous tension and stress-related conditions. In the present study, we identified the effects of the juniper volatile oil on amyloid beta (1-42)-induced oxidative stress in the rat hippocampus. Rats received a single intracerebroventricular injection of amyloid beta (1-42) (400 pmol/rat) and then were exposed to juniper volatile oil (200 μl, either 1 or 3 %) for controlled 60 min period, daily, for 21 continuous days. Also, the antioxidant activity in the hippocampus was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels. Additionally, the acetylcholinesterase activity in the hippocampus was assessed. The amyloid beta (1-42)-treated rats exhibited the following: increase of the acetylcholinesterase, superoxide dismutase and catalase specific activities, decrease of glutathione peroxidase specific activity and the total content of the reduced glutathione along with an elevation of malondialdehyde and protein carbonyl levels. Inhalation of the juniper volatile oil significantly decreases the acetylcholinesterase activity and exhibited antioxidant potential. These findings suggest that the juniper volatile oil may be a potential candidate for the development of therapeutic agents to manage oxidative stress associated with Alzheimer's disease through decreasing the activity of acetylcholinesterase and anti-oxidative mechanism.
Faculty of Pharmacy, University of Medicine and Pharmacy "Gr. T. Popa", 16 University Str., 700117, Iasi, Romania.
Oana
O
Cioanca
Monica
M
Hancianu
Marius
M
Mihasan
Lucian
L
Hritcu
D000110:Acetylcholinesterase / Q000378:metabolism; D000280:Administration, Inhalation; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000302:isolation & purification; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage* / Q000302:isolation & purification; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology*; D018653:Juniperus; D008297:Male; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000302:isolation & purification; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000633:toxicity*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D009822:Oils, Volatile; D010446:Peptide Fragments; D010936:Plant Extracts; C075222:amyloid beta-protein (1-42); D000110:Acetylcholinesterase