Vaginal Lactobacillus gasseri CMUL57 can inhibit herpes simplex type 2 but not Coxsackievirus B4E2.
This study aimed at demonstrating the antiviral activity of Lactobacillus gasseri CMUL57 (L. gasseri CMUL57), L. acidophilus CMUL67 and L. plantarum CMUL140 against herpes simplex type 2 (HSV-2) and Coxsackievirus B4E2 (CVB4E2), which are enveloped and naked viruses, respectively. These lactobacilli were non-cytotoxic and were able to reduce the cytopathic effect induced by HSV-2 in Vero cell monolayers. However, lactobacilli were not active against CVB4E2. Tested lactobacilli displayed anti-HSV-2 activity when they were co-incubated with the virus prior to inoculating the mixture to Vero cell monolayers. The detection of HSV-2 DNA by PCR in pellets of bacteria/virus mixtures let us to hypothesize that anti-HSV-2 activity of lactobacilli resulted from the viruses' entrapment. This study showed the capabilities of vaginal lactobacilli to inhibit enveloped viruses such as HSV-2.
Université de Lille 1 Sciences et Technologies, Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies, Institut Charles Viollette-Université Lille 1, Bâtiment Polytech-Lille, Bâtiment Polytech-Lille (Bureau C315), Cité Scientifique, Université de Lille, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France.
Imad Al
IA
Kassaa
Didier
D
Hober
Monzer
M
Hamze
Delphine
D
Caloone
Anny
A
Dewilde
Nour-Eddine
NE
Chihib
Djamel
D
Drider
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000254:growth & development; D005260:Female; D006558:Herpes Genitalis / Q000276:immunology* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000254:growth & development*; D006801:Humans; D007778:Lactobacillus / Q000276:immunology*; D014621:Vagina / Q000276:immunology / Q000382:microbiology* / Q000821:virology; D014709:Vero Cells; D010948:Viral Plaque Assay