[The influence of spermine on Ca(2+)-dependent permeability transition in mitochondria and liposomes induced by palmitic and α,Ω-hexadecanedioic acids].
The effect of spermine on Ca(2+)-dependent permeability transition in mitochondria and liposomes induced by palmitic and α,Ω-hexadecanedioic acid was studied. It has been shown that spermine inhibited the cyclosporin A-insensitive mitochondrial swelling induced by palmitic acid and Ca2+ and α,Ω-hexadecanedioic acid and Ca2+. 100 μM spermine did not influence the mitochondrial respiration in state V2 and the respiration stimulated by palmitic acid, α,Ω-hexadecanedioic acid and Ca2+. Pre-incubation of liposomes with 100 μM spermine resulted in the inhibition of palmitic acid/Ca(2+)- and α,Ω-hexadecanedioic acid/Ca(2+)-induced release of the fluorescent dye sulforhodamine B from liposomes. At the same time, spermine added to fatty acids-contained membranes of liposomes stimulated Ca(2+)-dependent release of sulforhodamine B from liposomes. It was shown that an addition of spermine to liposomes resulted in a significant increase in z-potential of liposomal membranes (from -39.8 mV to -18.6 mV). A possible mechanism of spermine influence on palmitic acid/Ca(2+)- and α,Ω-hexadecanedioic acid/Ca(2+)-induced permeability transition in mitochondria and liposomes is discussed.
K N
KN
Belosludtsev
N V
NV
Belosludtseva
M V
MV
Dubinin
S V
SV
Gudkov
N V
NV
Pen'kov
V N
VN
Samartsev
D000818:Animals; D002118:Calcium / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D008930:Mitochondria, Liver / Q000737:chemistry*; D051336:Mitochondrial Membranes / Q000737:chemistry*; D010169:Palmitic Acids / Q000737:chemistry*; D010539:Permeability; D051381:Rats; D013096:Spermine / Q000737:chemistry*
D008081:Liposomes; D010169:Palmitic Acids; D013096:Spermine; C012346:hexadecanedioic acid; D002118:Calcium