Highly specific enrichment of N-glycoproteome through a nonreductive amination reaction using Fe3O4@SiO2-aniline nanoparticles.
A novel method based on the conjunction of aldehydes from oxidized glycopeptides to aniline groups on magnetic nanoparticles via nonreductive amination is reported for the highly selective enrichment of N-glycopeptides. For the first time, a nonreductive amination reaction has been introduced into N-glycoproteome extraction, and correspondingly a new type of aniline-functionalized nanoparticle has been designed and synthesized.
Shanghai Cancer Center and Department of Chemistry, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn.
Ying
Y
Zhang
Meng
M
Yu
Cheng
C
Zhang
Yali
Y
Wang
Yi
Y
Di
Changchun
C
Wang
Haojie
H
Lu
D000447:Aldehydes / Q000737:chemistry; D000586:Amination; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D001212:Asialoglycoproteins / Q000302:isolation & purification*; D001798:Blood Proteins / Q000737:chemistry*; D002417:Cattle; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D060748:Fetuins / Q000302:isolation & purification*; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D020543:Proteome / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods
D000447:Aldehydes; D000814:Aniline Compounds; D001212:Asialoglycoproteins; D001798:Blood Proteins; D060748:Fetuins; D006020:Glycopeptides; D058185:Magnetite Nanoparticles; D020543:Proteome; C017029:asialofetuin; D012822:Silicon Dioxide; C023650:aniline; D052203:Ferrosoferric Oxide