Protective effect of hispidulin on kainic acid-induced seizures and neurotoxicity in rats.
Hispidulin is a flavonoid compound which is an active ingredient in a number of traditional Chinese medicinal herbs, and it has been reported to inhibit glutamate release. The purpose of this study was to investigate whether hispidulin protects against seizures induced by kainic acid, a glutamate analog with excitotoxic properties. The results indicated that intraperitoneally administering hispidulin (10 or 50mg/kg) to rats 30 min before intraperitoneally injecting kainic acid (15 mg/kg) increased seizure latency and decreased seizure score. In addition, hispidulin substantially attenuated kainic acid-induced hippocampal neuronal cell death, and this protective effect was accompanied by the suppression of microglial activation and the production of proinflammatory cytokines such as interleukin-1β, interleukin-6, and tumor necrosis factor-α in the hippocampus. Moreover, hispidulin reduced kainic acid-induced c-Fos expression and the activation of mitogen-activated protein kinases in the hippocampus. These data suggest that hispidulin has considerable antiepileptic, neuroprotective, and antiinflammatory effects on kainic acid-induced seizures in rats.
Department of Anesthesiology, Far-Eastern Memorial Hospital, New Taipei City, Taiwan; Department of Mechanical Engineering, Yuan Ze University, Taoyuan, Taiwan.
Tzu Yu
TY
Lin
Department of Anesthesiology, Far-Eastern Memorial Hospital, New Taipei City, Taiwan; Department of Mechanical Engineering, Yuan Ze University, Taoyuan, Taiwan.
Cheng Wei
CW
Lu
Graduate Institute of Basic Medicine, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang District, New Taipei City 24205, Taiwan. Electronic address: med0003@mail.fju.edu.tw.
Su Jane
SJ
Wang
Department of Anesthesiology, Far-Eastern Memorial Hospital, New Taipei City, Taiwan.
Shu Kuei
SK
Huang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use*; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D016207:Cytokines / Q000235:genetics; D047309:Flavones / Q000494:pharmacology / Q000627:therapeutic use*; D007608:Kainic Acid; D008297:Male; D017628:Microglia / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D000893:Anti-Inflammatory Agents; D000927:Anticonvulsants; D016207:Cytokines; D047309:Flavones; D018696:Neuroprotective Agents; D012333:RNA, Messenger; C055957:hispidulin; D007608:Kainic Acid
Anti-inflammation; Antiepileptic effect; Hippocampus; Hispidulin (PubChem CID:5281628); Kainic acid (PubChem CID:10255); Neuroprotection