Ethanol modulates the synthesis and catabolism of retinoic acid in the rat prostate.
All-trans retinoic acid (atRA) maintains physiological stability of the prostate, and we reported that ethanol intake increases atRA in the rat prostate; however the mechanisms underlying these changes are unknown. We evaluated the impact of a low- and high-dose ethanol intake (UChA and UChB strains) on atRA metabolism in the dorsal and lateral prostate. Aldehyde dehydrogenase (ALDH) subtype 1A3 was increased in the dorsal prostate of UChA animals while ALDH1A1 and ALDH1A2 decreased in the lateral prostate. In UChB animals, ALDH1A1, ALDH1A2, and ALDH1A3 increased in the dorsal prostate, and ALDH1A3 decreased in the lateral prostate. atRA levels increased with the low activity of CYP2E1 and decreased with high CYP26 activity in the UChB dorsal prostate. Conversely, atRA was found to decrease when the activity of total CYP was increased in the UChA lateral prostate. Ethanol modulates the synthesis and catabolism of atRA in the prostate in a concentration-dependent manner.
Department of Anatomy, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil; Structural and Cell Biology Program, UNICAMP, Campinas, SP, Brazil.
Beatriz Aparecida
BA
Fioruci-Fontanelli
Department of Anatomy, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil. Electronic address: chuffa@ibb.unesp.br.
Luiz Gustavo A
LG
Chuffa
Department of Anatomy, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil; Structural and Cell Biology Program, UNICAMP, Campinas, SP, Brazil.
Leonardo O
LO
Mendes
Department of Anatomy, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
Patricia Fernanda F
PF
Pinheiro
Department of Morphology, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
Luis Antônio
LA
Justulin
Department of Morphology, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
Sérgio Luis
SL
Felisbino
Department of Anatomy, Institute of Biosciences, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
Francisco Eduardo
FE
Martinez
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D050697:Retinal Dehydrogenase / Q000378:metabolism; D014212:Tretinoin / Q000493:pharmacokinetics*
D000431:Ethanol; D014212:Tretinoin; D003577:Cytochrome P-450 Enzyme System; D050697:Retinal Dehydrogenase
ALDH; All-trans retinoic acid; CYP; Ethanol; Prostate