Electrogenerated chemiluminescence biosensing for the detection of prostate PC-3 cancer cells incorporating antibody as capture probe and ruthenium complex-labelled wheat germ agglutinin as signal probe.
A highly selective and sensitive electrogenerated chemiluminescence (ECL) biosensor for the detection of prostate PC-3 cancer cells was designed using a prostate specific antibody as a capture probe and ruthenium complex-labelled wheat germ agglutinin as a signal probe. The ECL biosensor was fabricated by covalently immobilising the capture probe on a graphene oxide-coated glassy carbon electrode. Target PC-3 cells were selectively captured on the surface of the biosensor, and then, the signal probe was bound with the captured PC-3 cells to form a sandwich. In the presence of tripropylamine, the ECL intensity of the sandwich biosensor was logarithmically directly proportion to the concentration of PC-3 cells over a range from 7.0×10(2) to 3.0×10(4) cells mL(-1), with a detection limit of 2.6×10(2) cells mL(-1). The ECL biosensor was also applied to detect prostate specific antigen with a detection limit of 0.1 ng mL(-1). The high selectivity of the biosensor was demonstrated in comparison with that of a lectin-based biosensor. The strategy developed in this study may be a promising approach and could be extended to the design of ECL biosensors for highly sensitive and selective detection of other cancer-related cells or cancer biomarkers using different probes.
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China; Department of Chemistry, Yuncheng University, Yuncheng 044300, PR China.
Haiying
H
Yang
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Zhejian
Z
Li
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Meng
M
Shan
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Congcong
C
Li
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Honglan
H
Qi
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Qiang
Q
Gao
College of Science and College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
Jinyi
J
Wang
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China. Electronic address: cxzhang@snnu.edu.cn.
Chengxiao
C
Zhang
D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004566:Electrodes; D006801:Humans; D049449:Luminescence; D008297:Male; D015335:Molecular Probes / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012428:Ruthenium / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D000906:Antibodies; D015335:Molecular Probes; D009942:Organometallic Compounds; D014909:Wheat Germ Agglutinins; D002244:Carbon; D012428:Ruthenium
Biosensor; Electrogenerated chemiluminescence; Graphene oxide; Prostate PC-3 cancer cells; Wheat germ agglutinin