Effects of prebiotic supplementation on the expression of proteins regulating iron absorption in anaemic growing rats.
Prebiotics may increase intestinal Fe absorption in anaemic growing rats. The present study evaluated the effects of high-performance (HP) inulin and oligofructose on factors that regulate Fe absorption in anaemic rats during the growth phase. Male Wistar rats aged 21 d of age were fed AIN-93G ration without Fe for 2 weeks to induce Fe-deficiency anaemia. The rats were fed on day 35 a control diet, or a diet with 10 % HP inulin, or a diet with 10 % oligofructose, without Fe supplementation. The animals were euthanised after 2 weeks, and segments of the duodenum, caecum, colon and liver were removed. The expression levels of proteins in the intestinal segments were assessed using Western blotting. The levels of serum, urine and liver hepcidin and the concentrations of IL-10, IL-6 and TNF-α in the caecum, colon and liver were measured using the ELISA test. HP inulin increased the expression of the divalent metal transporter 1 protein in the caecum by 162 % (P= 0·04), and the expression of duodenal cytochrome b reductase in the colon by 136 % (P= 0·02). Oligofructose decreased the expression of the protein ferroportin in the duodenum (P= 0·02), the concentrations of IL-10 (P= 0·044), IL-6 (P= 0·036) and TNF-α (P= 0·004) in the caecum, as well as the level of urinary hepcidin (P< 0·001). These results indicate that prebiotics may interfere with the expression of various intestinal proteins and systemic factors involved in the regulation of intestinal Fe absorption in anaemic rats during the growth phase.
Department of Physiology,Physiology of Nutrition Discipline, Federal University of São Paulo,Rua Botucatu 862, 2nd floor,04023-060São Paulo,Brazil.
Renata
R
Marciano
Department of Physiology,Physiology of Nutrition Discipline, Federal University of São Paulo,Rua Botucatu 862, 2nd floor,04023-060São Paulo,Brazil.
Aline Boveto
AB
Santamarina
Department of Physiology,Physiology of Nutrition Discipline, Federal University of São Paulo,Rua Botucatu 862, 2nd floor,04023-060São Paulo,Brazil.
Aline Alves
AA
de Santana
Department of Pediatrics,Pediatric Gastroenterology Discipline, Federal University of São Paulo,Rua Coronel Lisboa, 826,04020-000São Paulo,SP,Brazil.
Maísa de Lima Correia
Mde L
Silva
Department of Pediatrics,Research Laboratory of the Nutrology Discipline, Federal University of São Paulo,Rua Botucatu, 703,04023-062São Paulo,SP,Brazil.
Olga Maria Silvério
OM
Amancio
Department of Physiology,Physiology of Nutrition Discipline, Federal University of São Paulo,Rua Botucatu 862, 2nd floor,04023-060São Paulo,Brazil.
Claudia Maria da Penha Oller
CM
do Nascimento
Department of Physiology,Physiology of Nutrition Discipline, Federal University of São Paulo,Rua Botucatu 862, 2nd floor,04023-060São Paulo,Brazil.
Lila Missae
LM
Oyama
Department of Pediatrics,Federal University of São Paulo,Rua Botucatu, 598,04023-062São Paulo,SP,Brazil.
Mauro Batista
MB
de Morais
D018798:Anemia, Iron-Deficiency / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D027682:Cation Transport Proteins / Q000819:agonists / Q000378:metabolism*; D002432:Cecum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D003573:Cytochrome b Group / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004386:Duodenum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D064451:Hepcidins / Q000097:blood / Q000378:metabolism / Q000652:urine; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007444:Inulin / Q000009:adverse effects / Q000627:therapeutic use; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D009844:Oligosaccharides / Q000009:adverse effects / Q000627:therapeutic use; D009929:Organ Size; D056692:Prebiotics / Q000009:adverse effects; D017208:Rats, Wistar; D015854:Up-Regulation; D015430:Weight Gain; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D003573:Cytochrome b Group; D064451:Hepcidins; D018836:Inflammation Mediators; D007444:Inulin; D009844:Oligosaccharides; D056692:Prebiotics; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2; C000598701:Cybrd1 protein, rat; C120489:oligofructose
Rats