[High-intensity focused ultrasound inhibits tumor metastasis in a melanoma-bearing mouse model].
OBJECTIVE
To investigate the effect of high-intensity focused ultrasound (HIFU) on tumor metastasis in mouse model bearing melanoma xenograft.


METHODS
Mice bearing murine melanoma B16-F10 cell xenograft were randomized for sham-HIFU or HIFU exposure when the tumors grew to a maximum diameter of 7-10 mm, and the tumor size was measured every 3 days. The cumulative survival rate of the mice and tumor metastasis rate were calculated, and the circulating melanoma cells were detected using qRT-PCR. At 14 days after HIFU treatment, B16-F10 cells were retransplanted via the tail vein and the pulmonary metastatic nodules were counted.


RESULTS
The median survival time of the mice was 19.00 days (95% CI 17.14-20.86 days) in the sham group and 26.00 days (95%CI 24.76-27.25 days) in HIFU group. The cumulative survival rate in the HIFU group was significantly higher than that in sham-HIFU group (P<0.01), and the tumor size was significantly smaller in HIFU group at 20, 23, and 26 days after HIFU treatment (P<0.05). Compared with the sham-HIFU group, HIFU group had significantly lower levels of MAGE-A3, MART1 and PAX3 at 7 days after HIFU (P<0.05) with still lower MAGE-A3 level at 14 days (P<0.05). HIFU group showed a significantly smaller number of pulmonary metastatic nodules following tumor cell retransplantation than in sham-HIFU group (P<0.01) with a metastasis inhibition rate of 42.4%.


CONCLUSIONS
HIFU treatment can inhibit tumor metastasis in melanoma-bearing mice possibly by reducing tumor cell detachment from the primary tumor site and suppressing colonization of the circulating melanoma cells.
Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Clinical Laboratory Diagnostics, Chongqing Medical University, Chongqing 400016, China.E-mail: 729835071@qq.com.
Huan
H
Li
Shimei
S
Yuan
Min
M
Yang
Liang
L
Duan
Haiyan
H
Wang
He
H
Zha
Xueru
X
Li
Hui
H
Sun
Yaguang
Y
Weng
Jinyong
J
Luo
Tongchuan
T
He
Chongyan
C
Li
Yan
Y
Wang
Faqi
F
Li
Zhibiao
Z
Wang
Lan
L
Zhou
D000818:Animals; D057086:High-Intensity Focused Ultrasound Ablation; D008546:Melanoma, Experimental / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D015996:Survival Rate