Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.
Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments.
Tsukuba Research Laboratories, Aquas Corporation, 4–4 Midorigahara, Tsukuba, Ibaraki 300–2646, Japan; Ibaraki University College of Agriculture, 3–21–1 Chuou, Ami-machi, Ibaraki 300–0393, Japan.
Hiroaki
H
Inoue
Reiko
R
Fujimura
Kunio
K
Agata
Hiroyuki
H
Ohta
D001386:Azides / Q000378:metabolism; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000378:metabolism; D014644:Genetic Variation; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D001386:Azides; D004275:DNA, Ribosomal; D004791:Enzyme Inhibitors; D012336:RNA, Ribosomal, 16S; C014535:8-azidoethidium