MicroRNA expression in differentially metastasizing tumors of the head and neck: adenoid cystic versus squamous cell carcinoma.
OBJECTIVE
Head and neck adenoid cystic carcinoma (HNACC) is a rare malignancy of the salivary glands with a tendency to metastasize in lung or liver without lymph node involvement, whereas squamous cell carcinoma (HNSCC) preferentially metastasizes to locoregional lymph nodes. The expression patterns of microRNA, a class of small non-coding RNA transcripts, involved in gene regulation and various developmental processes, could be of influence during the metastatic process. The aim of the present study was to compare mircoRNA expression patterns of HNACC and HNSCC.


METHODS
In a total of 21 tissue samples, a genome-wide screening for microRNAs was performed. A microRNA array platform was used for the identification of target microRNA.


RESULTS
Five microRNAs, hsa-MiR-214, hsa-MiR-125a-5p, hsa-MiR-574-3p, hsa-MiR-199a-3p/199b-3p and hsa-miR-199a-5p were identified to be over-expressed in HNACC compared to HNSCC, whereas hsa-MiR-452 showed a lower expression level.


CONCLUSIONS
Our data showed significantly different expression patterns of mircoRNA in HNACC and HNSCC supporting the theory of tumor-specific expression and giving hints for different clinical behavior.
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany johannes.veit@uniklinik-ulm.de.
Johannes A
JA
Veit
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Düsseldorf University Medical Center, Düsseldorf, Germany.
Kathrin
K
Scheckenbach
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany.
Patrick J
PJ
Schuler
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany.
Simon
S
Laban
Paul Severin
PS
Wiggenhauser
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany.
Severin P
SP
Wiggenhauser
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany.
Julia
J
Thierauf
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Giessen University Medical Center, Giessen, Germany.
Jens P
JP
Klussmann
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Ulm University Medical Center, Ulm, Germany.
Thomas K
TK
Hoffmann
D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000556:secondary; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D000077195:Squamous Cell Carcinoma of Head and Neck
D035683:MicroRNAs
HPV; adenoid cystic carcinoma; head and neck squamous cell carcinoma; microRNA