The influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats: A histometric and immunohistochemical evaluation.
OBJECTIVE
Although it has been shown that rifamycin is an effective agent for bone graft decontamination, no information exists on the effects of rifamycin decontamination on bone graft incorporation. The aim of this study was to evaluate the influence of rifamycin decontamination on the incorporation of autologous onlay bone grafts quantitatively.


METHODS
In 30 rats, a standardized 5.0-mm-diameter bone graft was harvested from the right mandibular angle, contaminated with saliva, decontaminated with rifamycin solution, and augmented to the left as an onlay graft. Ten animals were sacrificed at 7, 14, and 21 days after surgery. In the control group (10 rats), the onlay grafts were neither contaminated nor decontaminated, and the rats were sacrificed at 21 days after surgery. Histological slides were prepared from each grafted site for both immunohistochemistry analysis (bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) antibodies) and histometric analysis. Images obtained from the graft incorporation area with the light microscope were transferred to a PC, and they were evaluated using Clemex PE 3.5 image analysis software.


RESULTS
The grafts were fully incorporated in all specimens. The results showed that rifamycin decontamination has no detrimental effect on graft incorporation and the findings revealed a tendency for earlier revascularization and osteogenesis in the decontamination group. Data were analyzed using variance analysis and Tukey's test.


CONCLUSIONS
Rifamycin decontamination has no detrimental effect on autogenous graft incorporation, and it can be used for graft decontamination with confidence.
Dentistry Faculty, Oral and Maxillofacial Department, Pamukkale University, Denizli, Turkey. Electronic address: Ufukdt@gmail.com.
Ufuk
U
Taşdemir
Dentistry Faculty, Oral and Maxillofacial Department, Cumhuriyet University, Sivas, Turkey.
İlker
İ
Özeç
Medicine Faculty, Pathology Department, Necmettin Erbakan University, Konya, Turkey.
Haci Hasan
HH
Esen
Medicine Faculty, Pathology Department, Necmettin Erbakan University, Konya, Turkey.
M Cihat
MC
Avunduk
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D016025:Bone Transplantation; D003666:Decontamination / Q000379:methods*; D007150:Immunohistochemistry; D008334:Mandible / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D012294:Rifamycins / Q000494:pharmacology*; D014182:Transplantation, Autologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D012294:Rifamycins; D042461:Vascular Endothelial Growth Factor A
Autogenous bone block graft; Decontamination; Rifamycin