Preliminary studies on application of library of artificial receptors for differentiation of metabolites in urine of healthy and cancer bearing mice.
A library of artificial receptors formed by the self-organization of N-lipidated peptides attached to the cellulose via m-aminophenylamino-1,3,5-triazine was used for differentiation of metabolites in urine of healthy and cancer bearing mice. The interactions of urine metabolites with the receptors were visualized by using competitive adsorption-desorption of an appropriate reporter dye. Analysis of the binding pattern (fingerprint) of urine metabolites from healthy and from cancer suffering mice showed that there were several structures among 120-elements molecular receptors which were able to differentiate bonded ligands depending on the healthy state. For all three tested types of cancers two structures: Lipid-Pro-Ala-NH-C6H4-NH-DMT-cellulose and Lipid-Arg-Pro-NH-C6H4-NH-DMT-cellulose were selected as diagnostic.
Małgorzata
M
Walczak
Justyna
J
Frączyk
Zbigniew J
ZJ
Kamiński
Joanna
J
Wietrzyk
Beata
B
Filip-Psurska
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002482:Cellulose / Q000737:chemistry; D005260:Female; D008024:Ligands; D055666:Lipopeptides / Q000737:chemistry / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000652:urine; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D019151:Peptide Library; D011485:Protein Binding; D062165:Receptors, Artificial / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry; D014556:Urine / Q000737:chemistry
D008024:Ligands; D055666:Lipopeptides; D019151:Peptide Library; D062165:Receptors, Artificial; D014227:Triazines; C542080:m-aminophenylamino-1,3,5-triazine; D002482:Cellulose