Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis.
To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p≤0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.
Department of Prosthodontics, Faculty of Dentistry, İnönü University.
Meral
M
Arslan Malkoç
Necla
N
Demir
Abdulkadir
A
Şengün
Şerife Buket
ŞB
Bozkurt
Sema Sezen
SS
Hakki
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D008422:Materials Testing; D019279:Resin Cements / Q000009:adverse effects* / Q000494:pharmacology
C515955:Maxcem; D019279:Resin Cements