In silico analysis of usher encoding genes in Klebsiella pneumoniae and characterization of their role in adhesion and colonization.
Chaperone/usher (CU) assembly pathway is used by a wide range of Enterobacteriaceae to assemble adhesive surface structures called pili or fimbriae that play a role in bacteria-host cell interactions. In silico analysis revealed that the genome of Klebsiella pneumoniae LM21 harbors eight chromosomal CU loci belonging to γκп and ϭ clusters. Of these, only two correspond to previously described operons, namely type 1 and type 3-encoding operons. Isogenic usher deletion mutants of K. pneumoniae LM21 were constructed for each locus and their role in adhesion to animal (Intestine 407) and plant (Arabidopsis thaliana) cells, biofilm formation and murine intestinal colonization was investigated. Type 3 pili usher deleted mutant was impaired in all assays, whereas type 1 pili usher deleted mutant only showed attenuation in adhesion to plant cells and in intestinal colonization. The LM21ΔkpjC mutant was impaired in its capacity to adhere to Arabidopsis cells and to colonize the murine intestine, either alone or in co-inoculation experiments. Deletion of LM21kpgC induced a significant decrease in biofilm formation, in adhesion to animal cells and in colonization of the mice intestine. The LM21∆kpaC and LM21∆kpeC mutants were only attenuated in biofilm formation and the adhesion abilities to Arabidopsis cells, respectively. No clear in vitro or in vivo effect was observed for LM21∆kpbC and LM21∆kpdC mutants. The multiplicity of CU loci in K. pneumoniae genome and their specific adhesion pattern probably reflect the ability of the bacteria to adhere to different substrates in its diverse ecological niches.
LMGE-UMR CNRS 6023- Clermont Ferrand, 63000, France.
Fida
F
Khater
LMGE-UMR CNRS 6023- Clermont Ferrand, 63000, France.
Damien
D
Balestrino
LMGE-UMR CNRS 6023- Clermont Ferrand, 63000, France.
Nicolas
N
Charbonnel
CIRAD, UMR 1334 AGAP, Avenue Agropolis, 34398 Montpellier, France.
Jean François
JF
Dufayard
Institut Pasteur, Microbial Evolutionary Genomics, 75015 Paris, France; CNRS, UMR 3525, Paris, France.
Sylvain
S
Brisse
LMGE-UMR CNRS 6023- Clermont Ferrand, 63000, France.
Christiane
C
Forestier
D000818:Animals; D017360:Arabidopsis / Q000382:microbiology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms; D002460:Cell Line; D005260:Female; D015870:Gene Expression; D005798:Genes, Bacterial; D056426:Genetic Loci; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics*; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny
D001426:Bacterial Proteins; D018832:Molecular Chaperones