Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands.
To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p = 0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p < 0.001).
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands. Electronic address: itmaoverdevest@gmail.com.
I T M A
IT
Overdevest
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands; Laboratory for Medical Microbiology, Franciscus Hospital, Roosendaal, The Netherlands.
A M C
AM
Bergmans
Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
J J
JJ
Verweij
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
J
J
Vissers
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
N
N
Bax
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
E
E
Snelders
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands; Julius Centre, University Medical Centre, Utrecht, The Netherlands.
J A J W
JA
Kluytmans
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009426:Netherlands / Q000453:epidemiology; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D065288:Serogroup; D001618:beta-Lactamases / Q000378:metabolism
D001618:beta-Lactamases
Blood culture; E. coli; ST131; phylogroups; rectal colonization; urine