[In vitro generation of insulin-producing cells from the neonatal rat bone marrow mesenchymal stem cells].
OBJECTIVE
To observe the differentiation of the neonatal rat bone marrow mesenchymal stem cells (MSCs) into insulin-producing cells and detect the expressions of insulin, pancreatic duodenal homebox-1 (PDX-1) and nestin.


METHODS
MSCs were isolated from the neonatal rats and cultured in the modified medium composed of 10 μg/L human epidermal growth factor (EGF), 10 μg/L basic fibroblast growth factor (bFGF), 10 μg/L hepatocyte growth factor (HGF), 10 μg/L human B cell regulin, 20 mmol/L nicotinamide and 20 g/L B27. After the induction, the mRNA expressions of insulin, PDX-1 and nestin were examined by reverse transcription-PCR, and the insulin, PDX-1 and nestin protein levels were detected by immunocytochemistry.


RESULTS
The insulin and PDX-1 mRNA expressions increased and the nestin mRNA expression decreased in the differentiation of the neonatal rat MSCs into insulin-producing cells. The nestin, PDX-1 and insulin proteins were co-expressed in insulin-producing cells.


CONCLUSIONS
MSCs can be induced to differentiate into insulin-producing cells.
School of Medicine, Hubei University of Science and Technology, First People's Hospital of Xianning City, Xianning 437100, China.
Xiaohu
X
Li
School of Medicine, Hubei University of Science and Technology, First People's Hospital of Xianning City, Xianning 437100, China.
Haiyan
H
Huang
School of Medicine, Hubei University of Science and Technology, First People's Hospital of Xianning City, Xianning 437100, China.
Xirong
X
Liu
School of Medicine, Hubei University of Science and Technology, Central Hospital of Xianning City, Xianning 437100, China.
Hongxia
H
Xia
School of Medicine, Hubei University of Science and Technology, First People's Hospital of Xianning City, Xianning 437100, China.
Mincai
M
Li
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D018398:Homeodomain Proteins; D007328:Insulin; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein