Isolation and structural elements of a water-soluble free radical scavenger from Nyctanthes arbor-tristis leaves.
The leaves of Nyctanthes arbor-tristis L. (Oleaceae) are used in Ayurvedic medicine for the management of a range of diseases, but reports on its phytochemicals and pharmacological properties are inadequate. Herein, we report purification of an antioxidative polysaccharide (F2) extracted from its leaves by water. The presence of a highly branched polysaccharide (75 kDa) containing esterified phenolic acids was revealed by chemical, chromatographic and spectroscopic analyses. Particularly, ESMS analysis of per acetylated oligomeric fragments derived by Smith degradation provides important structural information on a spectrum of glycerol tagged oligosaccharides. This polysaccharide showed dose dependent free radical scavenging capacity as evidenced by DPPH and Ferric reducing power assay. This pharmacologically active compound (F2) formed a water soluble complex with bovine serum albumin over pH 4.0-7.4. Accordingly, traditional aqueous extraction method provides a molecular entity that induces a pharmacological effect: this could epitomize a smart approach in phytotherapeutic management.
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713 104, India.
Kanika
K
Ghosh
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713 104, India.
Sayani
S
Ray
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713 104, India.
Kaushik
K
Bera
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713 104, India. Electronic address: bray@chem.buruniv.ac.in.
Bimalendu
B
Ray
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D029592:Oleaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D011134:Polysaccharides; D014867:Water; D012710:Serum Albumin, Bovine
Antioxidative effect; BSA–polysaccharide interaction; Esterified phenolic acids; Nyctanthes arbor-tristis L.; Oleaceae; Polysaccharide; Smith degradation