Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice.
OBJECTIVE
Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability.


METHODS
To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting.


RESULTS
The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduced by an ERK1/2 inhibitor and the level of ERK1/2 phosphorylation was increased by treatment with Vd3.


CONCLUSIONS
This has been the first report on CM from Vd3 pre-activated preadipocyte displaying stimulatory effects on hair growth via the enhancement of angiogenesis in a hairless-induced C57BL/6 mice.
Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea.
Min Kyung
MK
Jung
Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea.
Soogyeong
S
Ha
Department of Biological Sciences, University of Connecticut, CT 06269-3042, USA.
Scarlett Yoona
SY
Huh
Biotech. Team, Cent'l Res. Inst., Ilyang Pharm., Co., Ltd., 359, Giheung-gu, Yongin-si, Gyeonggi-do, 449-726, Republic of Korea.
Seung Beom
SB
Park
Biotech. Team, Cent'l Res. Inst., Ilyang Pharm., Co., Ltd., 359, Giheung-gu, Yongin-si, Gyeonggi-do, 449-726, Republic of Korea.
Sangyoon
S
Kim
Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, Seoul 110-745, Republic of Korea.
Yoolhee
Y
Yang
Department of Anatomy, Inje University College of Medicine, Pusan 614-735, Republic of Korea.
Daejin
D
Kim
Department of Anatomy, Inje University College of Medicine, Pusan 614-735, Republic of Korea.
Dae Young
DY
Hur
Department of Chemistry, Sookmyung Women's University, Cheongpa-ro 47-gil 100, Yongsan-Ku, Seoul 140-742, Republic of Korea.
Hyuk
H
Jeong
Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, Seoul 110-745, Republic of Korea. Electronic address: si55.bang@samsung.com.
Sa Ik
SI
Bang
Department of Dermatology, St. Mary's Hospital, The Catholic University, Seoul 137-701, Republic of Korea. Electronic address: hjpark@catholic.ac.kr.
Hyunjeong
H
Park
Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea. Electronic address: cdhkor@sookmyung.ac.kr.
Daeho
D
Cho
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D006197:Hair / Q000187:drug effects / Q000254:growth & development*; D018859:Hair Follicle / Q000098:blood supply / Q000187:drug effects; D020935:MAP Kinase Signaling System; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D017077:Culture Media, Conditioned; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D002762:Cholecalciferol
Angiogenesis; Extracellular signal-regulated kinase 1/2; Hair growth; Preadipocyte; Vitamin D3