Localization of Banana bunchy top virus and cellular compartments in gut and salivary gland tissues of the aphid vector Pentalonia nigronervosa.
Banana bunchy top virus (BBTV) (Nanoviridae: Babuvirus) is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. Here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and principal salivary glands of Pentalonia nigronervosa. We applied colabeling assays, to colocalize BBTV viral particles with these cellular compartments and structures. Our results suggest that multiple potential cellular processes, including clathrin- and caveolae-mediated endocytosis and lipid rafts, may not be involved in BBTV internalization.
Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Gilmore Hall, 96822, Honolulu, HI, USA.|Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, HI, USA.
Shizu
S
Watanabe
Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, HI, USA.
Dulal
D
Borthakur
Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Gilmore Hall, 96822, Honolulu, HI, USA.
Alberto
A
Bressan
D000818:Animals; D001042:Aphids / Q000166:cytology / Q000821:virology*; D052658:Babuvirus; D041981:Gastrointestinal Tract / Q000166:cytology / Q000821:virology; D007303:Insect Vectors / Q000821:virology*; D028521:Musa / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D012469:Salivary Glands / Q000166:cytology / Q000821:virology
aphid digestive system; aphid salivary glands; cellular markers; circulative virus; immunofluorescence; transcytosis