Generation of med28 specific monoclonal antibodies.
Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo.
1 Laboratory for Tracing of Gene Function, College of Pharmacy, Ajou University , Suwon, Gyunggi-do, Korea.
Min A
MA
Yu
Jin Gu
JG
Cho
Kwang-Il
KI
Kim
Yeong Joon
YJ
Jo
Jong-Hyuk
JH
Sung
Ho Bin
HB
Yang
Sang Gyu
SG
Park
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000276:immunology; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D056892:Mediator Complex / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C496877:MED28 protein, human; D056892:Mediator Complex; D011994:Recombinant Proteins; D005620:Freund's Adjuvant