Isolation and characterization of tri- and tetra-repeat microsatellite loci in the white-spotted charr Salvelinus leucomaenis (Salmonidae).
Tri- and tetra-motif repeat microsatellite marker loci were developed for the white-spotted charr Salvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite-primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180-0·600 and 0·188-0·599, respectively.
National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321-1661, Japan.
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S
Yamamoto
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M
Sekino
D000483:Alleles; D000818:Animals; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014337:Trout / Q000235:genetics*
454 pyrosequencing; next-generation sequencer; polymorphism; short tandem repeat; simple sequence repeat