Creation of a functional graded nanobiomembrane using a new electrospinning system for drug release control and an in vitro validation of drug release behavior of the coating membrane.
Functional graded nanobiomembranes (FGMs) with multiple layers were created by a single process using a novel electrospinning system equipped with a generator and a PCI type motion board as a controller in order to control the drug release rate. By varying physical apparatus-related parameters such as nozzle-to-collector distance via a robot and the collector moving velocity the FGMs were formed. For the membrane base layer, poly-(ε-caprolactone) (PCL) with paclitaxel (PTX) was dissolved in a solvent (dichloromethane, N,N-dimethylformamide) and electrospun. For the top layers, the PCL solution was electrospun according to the distance and FGM system parameters, which can move the collector location at a constant ratio. It was observed that pore size, porosity, and permeability were higher when the membrane was spun at the far distance. The top surface of FGM is more porous, rougher, more permeable, and more hydrophilic so as to be active to the surrounding tissue cells. Meanwhile, the porous inside membrane was as low as the membrane spun at a close distance. Thus it induced a slow drug release due to the internal structure of FGM, which is considered to be very effective for slow drug release as well as bioactivity and bioconductivity.
Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Chan-Hee
CH
Park
Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Min-Young
MY
Chung
Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Afeesh Rajan
AR
Unnithan
Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.
Cheol Sang
CS
Kim
D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology*; D010539:Permeability; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water
D008567:Membranes, Artificial; D011091:Polyesters; D014867:Water; C016240:polycaprolactone; D017239:Paclitaxel
Drug release; Electrospinning; Functional graded membrane; Permeability; Stent coating