Continuous cultivation of Chlorella pyrenoidosa using anaerobic digested starch processing wastewater in the outdoors.
Microalgae cultivation using wastewater might be a suitable approach to support sustainable large-scale biomass production. Its compelling characteristics included the recycling of nutrients and water resources, reducing carbon emissions and harvesting available biomass. In outdoor batch and continuous cultures, Chlorella pyrenoidosa completely adapted to anaerobic digested starch processing wastewater and was the dominant microorganism in the photobioreactor. However, seasonal changes of environmental conditions significantly influenced biomass growth and lipid production. The long-term outdoor operation demonstrated that the biomass concentration and productivity in continuous operations at different hydraulic retention times (HRTs) can be successfully predicted using the kinetic growth parameters obtained from the batch culture. A moderate HRT (4days) in the summer provided the best microalgae and lipid production and achieved relatively high biomass concentrations of 1.29-1.62g/L, biomass productivities of 342.6±12.8mg/L/d and lipids productivities of 43.37±7.43mg/L/d.
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Hua-Qiang
HQ
Chu
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Xiao-Bo
XB
Tan
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China. Electronic address: zhangyalei@tongji.edu.cn.
Ya-Lei
YL
Zhang
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Li-Bin
LB
Yang
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Fang-Chao
FC
Zhao
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Jun
J
Guo
D000693:Anaerobiosis / Q000502:physiology; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002708:Chlorella / Q000502:physiology*; D010865:Pilot Projects; D013213:Starch / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology*
D013213:Starch; D062065:Wastewater
Anaerobic digested starch processing wastewater; Chlorella pyrenoidosa; Hydraulic retention time; Lipids production