Involvement of miRNAs and Cell-Secreted Vesicles in Mammalian Ovarian Antral Follicle Development.
Ovarian follicular development is a controlled series of events culminating with an ovulatory or atretic follicle. MicroRNAs (miRNAs) are small noncoding RNAs involved in translational regulation of genes in different developmental processes. Deletion of Dicer in mice ovaries demonstrated the importance of miRNAs in reproduction, which led to infertility. The miRNAs were thought to act only within host cells; however, these molecules are also present in cell-secreted vesicles. These vesicles are present in body fluids such as milk, serum, and ovarian follicular fluid. Vesicles are secreted in extracellular fluids and travel from donor to target cells, mediating transfer of bioactive material. Herein we discuss the role of hormonal-regulated miRNAs within different ovarian follicular cells as well as cell-secreted vesicles participation in mammalian ovarian follicular fluid. Furthermore, we discuss the possibility of miRNAs transference mediated by cell-secreted vesicles present in ovarian follicular fluid, increasing the versatility of miRNA functions during antral follicle development.
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil julianodasilveira@usp.br.
Juliano C
JC
da Silveira
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
Gabriella M
GM
de Andrade
Department of Biological Science, Faculty of Sciences and Letters, University of São Paulo State, Assis, São Paulo, Brazil.
Marcelo F G
MF
Nogueira
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
Flávio V
FV
Meirelles
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
Felipe
F
Perecin
D000818:Animals; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006062:Gonadotropins / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D022142:Secretory Vesicles / Q000378:metabolism*; D015398:Signal Transduction
D006062:Gonadotropins; D035683:MicroRNAs
cell-secreted vesicles; follicle; miRNAs; noncoding RNA; posttranscriptional gene regulation