Whole genome analysis of a Vietnamese trio.
We here present the first whole genome analysis of an anonymous Kinh Vietnamese (KHV) trio whose genomes were deeply sequenced to 30-fold average coverage. The resulting short reads covered 99.91 percent of the human reference genome (GRCh37d5). We identified 4,719,412 SNPs and 827,385 short indels that satisfied the Mendelian inheritance law. Among them, 109,914 (2.3 percent) SNPs and 59,119 (7.1 percent) short indels were novel. We also detected 30,171 structural variants of which 27,604 (91.5 percent) were large indels. There were 6,681 large indels in the range 0.1-100 kbp occurring in the child genome that were also confirmed in either the father or mother genome. We compared these large indels against the DGV database and found that 1,499 (22.44 percent) were KHV specific. De novo assembly of high-quality unmapped reads yielded 789 contigs with the length greater than or equal to 300 bp. There were 235 contigs from the child genome of which 199 (84.7 percent) were significantly matched with at least one contig from the father or mother genome. Blasting these 199 contigs against other alternative human genomes revealed 4 novel contigs. The novel variants identified from our study demonstrated the necessity of conducting more genome-wide studies not only for Kinh but also for other ethnic groups in Vietnam.
University of Engineering and Technology, Vietnam National University Hanoi, Hanoi, Vietnam.
Dang Thanh
DT
Hai
Nguyen Dai
ND
Thanh
Pham Thi Minh
PT
Trang
Le Si
le S
Quang
Phan Thi Thu
PT
Hang
Dang Cao
DC
Cuong
Hoang Kim
HK
Phuc
Nguyen Huu
NH
Duc
Do Duc
do D
Dong
Bui Quang
BQ
Minh
Pham Bao
PB
Son
Le Sy
le S
Vinh
D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics; D005006:Ethnicity / Q000235:genetics*; D005190:Family; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D014744:Vietnam
D004247:DNA