Hydrogen sulfide detection using nucleophilic substitution-cyclization-based fluorescent probes.
While previously known as a toxic pollutant, hydrogen sulfide (H2S) has been recently classified as an important signaling molecule with potent cytoprotective actions. The study on H2S is a rapidly growing field in biomedical research. Nevertheless, the detection of H2S in biological samples is still a challenge. Fluorescent assays can be very useful in this field owing to their high sensitivity and convenience. Based on the dual nucleophilicity of hydrogen sulfide, a series of reaction-based fluorescence "turn-on" probes have been developed by our lab. In this chapter, we provide an overview of the reactivity/property of these probes and detailed protocols for the use of them in experiments.
Department of Chemistry, Washington State University, Pullman, Washington, USA.
Bo
B
Peng
Department of Chemistry, Washington State University, Pullman, Washington, USA. Electronic address: mxian@wsu.edu.
Ming
M
Xian
D003500:Cyclization; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide
Fluorescence; Fluorescent probe; Hydrogen sulfide; Imaging; Nucleophilic reaction