Draft genome sequence of the Chilean isolate Aeromonas salmonicida strain CBA100.
We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest homologue to one third of the genes of strain CBA100 are found in A. hydrophila. The strain contains several efflux pumps and putative genes that confer resistance to multiclass antibiotics, including macrolide, β-lactamics, florfenicol and quinolones. The antibiogram profile suggests that efflux pumps are the main mechanism of resistance to non-β-lactamic antibiotics. This is the first genome of a Chilean isolate of A. salmonicida, which should shed light on the design of strain-specific vaccines against this pathogen and reduce the use of antibiotics for preventive treatment in Chilean aquaculture.
Unidad de Apoyo Bioinformático, Departamento de Biología, Facultad de Química y Biología, USACH, Alameda 3363, Santiago, Chile.
Natalia
N
Valdes
Laboratorio de Inmunología, Centro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología, USACH, Alameda 3363, Santiago, Chile.
Carolina
C
Espinoza
Laboratorio de Microbiología Básica y Aplicada, Departamento de Biología, Facultad de Química y Biología, USACH, Alameda 3363, Santiago, Chile.
Loreto
L
Sanhueza
Laboratorio de Microbiología Ambiental y Extremófilos, Departamento de Ciencias Biológicas y Biodiversidad, Universidad de los Lagos, Fuchslocher 1305, Osorno, Chile.
Alex
A
Gonzalez
Centro de Investigation Biomédica, Universidad Autónoma de Chile, Llano Subercaseaux 2801, Santiago, Chile.
Gino
G
Corsini
Laboratorio de Metagenómica Bacteriana, Centro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología, USACH, Alameda 3363, Santiago, Chile mario.tello@usach.cl.
Mario
M
Tello
D048409:Aeromonas salmonicida / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D017756:Aquaculture; D001482:Base Composition; D001483:Base Sequence; D002677:Chile; D002874:Chromosome Mapping; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology; D019450:Genes, MDR; D016680:Genome, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D008826:Microbial Sensitivity Tests; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA
fish pathogen; multiresistance; vaccine