Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on the Ovariectomized Mice.
Protective effect of new oriental medicine (Kyungokgo mixed with water extract of Hovenia dulcis, KOGHD) was assessed on the bone loss induced mice by ovariectomy. In the in vivo experiments, antiosteoporosis effect of KOGHD was investigated using ovariectomized osteoporosis mice model. After 6 weeks of treatment, the mice were euthanized, and the effect of Kyungokgo (KOG) and KOGHD on body weight, spleen weigh, thymus weight, uterine weight, serum biochemical indicators, bone weight and length, immune cell population, bone morphometric parameters, and histological stains was observed. Our results showed that KOGHD prevented the deterioration of trabecular microarchitecture caused by ovariectomy, which were accompanied by the lower levels of bone turnover markers and immune cell population as evidenced by the inhibition of RANKL-mediated osteoclast differentiation without cytotoxic effect on bone marrow derived macrophages (BMMs). Therefore, these results suggest that the Hovenia dulcis (HD) supplementation in the KOG may also prevent and treat bone loss.
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Yun-Ho
YH
Hwang
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kwang-Jin
KJ
Kim
Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
Jong-Jin
JJ
Kim
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kyung-Yun
KY
Kang
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Sung-Ju
SJ
Lee
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Gil-Yeon
GY
Jeong
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kyung-Hee
KH
Choi
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.
Young-Jin
YJ
Son
Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea ; Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
Sung-Tae
ST
Yee