Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China.
In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2.0) automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16-24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 130112, People's Republic of China.
Hewei
H
Zhang
Zhuo
Z
Zhang
Yufei
Y
Wang
Xin
X
Wang
Mingqi
M
Xia
Hua
H
Wu
D000818:Animals; D002460:Cell Line; D002681:China; D016000:Cluster Analysis; D003588:Cytopathogenic Effect, Viral; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004847:Epithelial Cells / Q000821:virology; D005838:Genotype; D060005:Genotyping Techniques; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D012367:RNA, Viral