Let-7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin.
Glioma is one of the most aggressive and malignant tumor types. Despite advances in surgery, imaging, chemotherapy, and radiation, glioma patient prognosis remains poor. Glioma pathogenesis is an urgent problem that must be solved. MicroRNAs (miRNAs) are endogenous small non-coding RNAs that are key post-transcriptional regulators of gene expression. miRNA deregulation commonly occurs in human tumorigenesis. In the present study, the expression levels of Let-7f were down-regulated in both glioma tissues and glioma cells. The enhanced expression of Let-7f suppressed glioma cells proliferation, migration, and invasion via direct targeting perisotin oncogenic activity. Experiments with periostin siRNA or over-expression further suggest that Let-7f may serve as tumor suppressors through perisotin signal. These findings provide insights regarding the role and mechanism of Let-7f in regulating biological behavior of glioma cells via the Let-7f/periostin axis, and Let-7f may serve as a potential therapeutic target in glioma.
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Shaofeng
S
Yan
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Xiao
X
Han
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Hao
H
Xue
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Ping
P
Zhang
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Xing
X
Guo
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Tong
T
Li
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Xiaofan
X
Guo
Department of Neurosurgery, Zibo Zhong Xin Hospital, Zibo, Shandong Province, P.R., China.
Guang
G
Yuan
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Lin
L
Deng
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, P.R., China.
Gang
G
Li
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D011401:Promoter Regions, Genetic
D015815:Cell Adhesion Molecules; D035683:MicroRNAs; C512835:POSTN protein, human; C487931:mirnlet7 microRNA, human
GLIOMA; INVASION; Let-7f; MIGRATION; PERIOSTIN; PROLIFERATION