Hsp40 function in yeast prion propagation: Amyloid diversity necessitates chaperone functional complexity.
Yeast prions are heritable protein-based elements, most of which are formed of amyloid aggregates that rely on the action of molecular chaperones for transmission to progeny. Prions can form distinct amyloid structures, known as 'strains' in mammalian systems, that dictate both pathological progression and cross-species infection barriers. In yeast these same amyloid structural polymorphisms, called 'variants', dictate the intensity of prion-associated phenotypes and stability in mitosis. We recently reported that [PSI(+)] prion variants differ in the fundamental domain requirements for one chaperone, the Hsp40/J-protein Sis1, which are mutually exclusive between 2 different yeast prions, demonstrating a functional plurality for Sis1. Here we extend that analysis to incorporate additional data that collectively support the hypothesis that Sis1 has multiple functional roles that can be accomplished by distinct sets of domains. These functions are differentially required by distinct prions and prion variants. We also present new data regarding Hsp104-mediated prion elimination and show that some Sis1 functions, but not all, are conserved in the human homolog Hdj1/DNAJB1. Importantly, of the 10 amyloid-based prions indentified to date in Saccharomyces cerevisiae, the chaperone requirements of only 4 are known, leaving a great diversity of amyloid structures, and likely modes of amyloid-chaperone interaction, largely unexplored.
a Department of Chemistry ; Lafayette College ; Easton , PA USA.
Zachary A
ZA
Sporn
Justin K
JK
Hines
D000682:Amyloid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011328:Prions / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000682:Amyloid; C494647:DNAJB1 protein, human; D050956:HSP40 Heat-Shock Proteins; D011328:Prions; C070320:SIS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
ATP; Hsp104; PIN; Rnq1; Ssa; adenosine triphosphate; SDSPAGE; cell stress; heat-shock; protein misfolding; semi-denaturing detergent agarose gel electrophoresis; sodium dodecyl sulfate polyacrylamide gel electrophoresis; SDDAGE