An analytical study on the common type of smokeless tobacco available in the Iranian market.
BACKGROUND
The use of smokeless tobacco is considered as a risk factor for oral cancer.


OBJECTIVE
The current study aimed to chemically analyze, separate and measure the existing substances in a type of chewing tobacco.


METHODS
In the current descriptive study, the investigated sample was a type of smokeless tobacco, known as BT, manufactured in India. First steam distillation method and Clevenger machine were used to separate and extract the essential oil of the sample. The presence of the desired compounds was evaluated in the essential oil, using gas chromatography (GC) and then gas chromatography coupled to mass spectrometry (GC/MS) analysis.


RESULTS
Based on the results obtained by the applied technique, the presence of carcinogenic compounds, N-nitrosomethyl-vinylamine (162 µg/g), N-nitrosonornicotine (6.3 µg/g), and Acetaldehyde (117 µg/g) was confirmed in the sample.


CONCLUSIONS
Chemical analysis of the sample confirmed the existence of carcinogenic compounds.
Department of Oral Medicine, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, IR Iran.
Leila
L
Farhadmollashahi
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, IR Iran.
Meisam
M
Noroozifar
Department of Chemistry, Faculty of Sciences, University of Shahrood, Shahrood, IR Iran.
Arezoo
A
Afroughe
Department of Pediatric, Faculty of Dentistry, Zahedn University of Medical Sciences, Zahedan, IR Iran.
Elahe Sadat
ES
Hashemi
Department of Oral Medicine, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, IR Iran.
Marieh
M
Honarmand
Analysis; Carcinogens; Smokeless; Tobacco