Identification of SHIP-1 and SHIP-2 homologs in channel catfish, Ictalurus punctatus.
Src homology domain 2 (SH2) domain-containing inositol 5'-phosphatases (SHIP) proteins have diverse roles in signal transduction. SHIP-1 and SHIP-2 homologs were identified in channel catfish, Ictalurus punctatus, based on sequence homology to murine and human SHIP sequences. Full-length cDNAs for catfish SHIP-1 and SHIP-2 (IpSHIP-1 and IpSHIP-2) were obtained using 5' and 3' RACE protocols. Catfish SHIP molecules share a high degree of sequence identity to their respective SHIP sequences from diverse taxa and both are encoded by single copy genes. IpSHIP-1 and IpSHIP-2 transcripts were expressed in all catfish tissues analyzed except for skin, and IpSHIP-1 message was more abundant than IpSHIP-2 message in lymphoid tissues. Catfish clonal B, cytotoxic T, and macrophage cell lines also expressed message for both molecules. IpSHIP-1 and IpSHIP-2 SH2 domains were expressed as recombinant proteins and were both found to be bound by cross-reacting rabbit anti-mouse SHIP-1 pAb. The anti-mouse SHIP-1 pAb also reacted with cell lysates from the cytotoxic T cell lines, macrophages and stimulated PBL. SHIP-1 is also phosphorylated at a conserved tyrosine residue, as shown by immunoprecipitation studies.
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Erin B
EB
Taylor
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Deepak K
DK
Nayak
Warmwater Aquaculture Research Unit, USDA-ARS, Stoneville, MS 38776, USA.
Sylvie M A
SM
Quiniou
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
Eva
E
Bengten
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA. Electronic address: mwilson@umc.edu.
Melanie
M
Wilson
D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000502:physiology*; D002460:Cell Line; D003429:Cross Reactions; D006801:Humans; D007059:Ictaluridae / Q000276:immunology*; D000072181:Inositol Polyphosphate 5-Phosphatases; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D018909:src Homology Domains / Q000235:genetics
D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases; C000606430:INPP5D protein, human; C000606433:Inpp5d protein, mouse; C000606435:Inppl1 protein, mouse; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
Phosphatases; Signal transduction