A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells.
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
Institute for Molecular Health Sciences, ETH Zurich, Zurich, Switzerland.|Molecular Life Science program, Life Science Zurich Graduate School, University of Zürich, Zurich, Switzerland.
Peter F
PF
Renz
Institute for Molecular Health Sciences, ETH Zurich, Zurich, Switzerland. tobias.beyer@biol.ethz.ch.|Department of Biology, Institute for Molecular Health Sciences, ETH Zurich, HPL E16, Otto-Stern-Weg 7, 8093, Zurich, Switzerland. tobias.beyer@biol.ethz.ch.|Lunenfeld-Tanenbaum Research Institute, Toronto, ON, Canada. tobias.beyer@biol.ethz.ch.
Tobias A
TA
Beyer
D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D000066449:Human Embryonic Stem Cells / Q000378:metabolism*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering
Gene silencing; Human embryonic stem cells; Lipofection; Transfection; siRNA mediated-knockdown