Fear-potentiated startle processing in humans: Parallel fMRI and orbicularis EMG assessment during cue conditioning and extinction.
Studying neural networks and behavioral indices such as potentiated startle responses during fear conditioning has a long tradition in both animal and human research. However, most of the studies in humans do not link startle potentiation and neural activity during fear acquisition and extinction. Therefore, we examined startle blink responses measured with electromyography (EMG) and brain activity measured with functional MRI simultaneously during differential conditioning. Furthermore, we combined these behavioral fear indices with brain network activity by analyzing the brain activity evoked by the startle probe stimulus presented during conditioned visual threat and safety cues as well as in the absence of visual stimulation. In line with previous research, we found a fear-induced potentiation of the startle blink responses when elicited during a conditioned threat stimulus and a rapid decline of amygdala activity after an initial differentiation of threat and safety cues in early acquisition trials. Increased activation during processing of threat cues was also found in the anterior insula, the anterior cingulate cortex (ACC), and the periaqueductal gray (PAG). More importantly, our results depict an increase of brain activity to probes presented during threatening in comparison to safety cues indicating an involvement of the anterior insula, the ACC, the thalamus, and the PAG in fear-potentiated startle processing during early extinction trials. Our study underlines that parallel assessment of fear-potentiated startle in fMRI paradigms can provide a helpful method to investigate common and distinct processing pathways in humans and animals and, thus, contributes to translational research.
Department of Psychology, University of Greifswald, Germany. Electronic address: katja.lindner@uni-greifswald.de.
Katja
K
Lindner
Department of Psychology, University of Greifswald, Germany.
Jörg
J
Neubert
Functional Imaging Unit, Center of Diagnostic Radiology and Neuroradiology, University of Greifswald, Germany.
Jörg
J
Pfannmöller
Functional Imaging Unit, Center of Diagnostic Radiology and Neuroradiology, University of Greifswald, Germany.
Martin
M
Lotze
Department of Psychology, University of Greifswald, Germany.
Alfons O
AO
Hamm
Department of Psychology, University of Greifswald, Germany.
Julia
J
Wendt
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001921:Brain / Q000098:blood supply* / Q000502:physiology; D003214:Conditioning, Classical / Q000502:physiology; D003463:Cues; D004576:Electromyography; D005108:Extinction, Psychological / Q000502:physiology*; D005239:Fear / Q000502:physiology*; D005260:Female; D005712:Galvanic Skin Response / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D008297:Male; D010100:Oxygen / Q000097:blood; D013216:Reflex, Startle / Q000502:physiology*; D057566:Self Report; D055815:Young Adult
D010100:Oxygen
Fear conditioning; Fear extinction; Fear-potentiated startle responses; fMRI