Single-step immobilization of cell adhesive peptides on a variety of biomaterial substrates via tyrosine oxidation with copper catalyst and hydrogen peroxide.
Immobilization of biologically active peptides which were isolated from extracellular matrix proteins is a powerful strategy for the design and functionalization of biomaterial substrates. However, the method of peptide immobilization was restricted, that is, peptide is often immobilized through the reactive groups inherent in substrates with multistep reactions. Here, we report a single-step immobilization of fibronectin-derived cell adhesive peptide (Arg-Glu-Asp-Val; REDV) onto polymer materials by use of tyrosine oxidation with copper catalyst and hydrogen peroxide. REDV peptide was successfully immobilized on tissue culture polystyrene, poly(ethylene terephthalate), poly(vinyl chloride), expanded-poly(tetrafluoroethylene), and poly(l-lactic acid), resulting in enhanced adhesion of human umbilical vein endothelial cells. This method is a single-step reaction under very mild conditions and is available for the biological functionalization of various medical devices.
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
Sachiro
S
Kakinoki
Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
Tetsuji
T
Yamaoka
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D002384:Catalysis; D002413:Cations, Divalent; D002448:Cell Adhesion; D003300:Copper / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010084:Oxidation-Reduction; D011091:Polyesters; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011143:Polyvinyl Chloride / Q000737:chemistry; D013499:Surface Properties; D014443:Tyrosine / Q000737:chemistry*
D001672:Biocompatible Materials; D002413:Cations, Divalent; D005353:Fibronectins; D055767:Immobilized Proteins; D009842:Oligopeptides; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011108:Polymers; D011137:Polystyrenes; D019344:Lactic Acid; D014443:Tyrosine; C033616:poly(lactide); D003300:Copper; D011143:Polyvinyl Chloride; C086684:arginyl-glycyl-aspartyl-valine; D006861:Hydrogen Peroxide