Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs.
Haemophilus parasuis, the causative agent of Glässer's disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer's disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria.
Instituto de Agrobiotecnología, CSIC-Universidad Pública Navarra-Gobierno de Navarra, Mutilva, Spain.
Javier
J
Moleres
Departamento de Sanidad Animal, Facultad de Veterinaria y VISAVET, Universidad Complutense, Madrid, Spain.
Alfonso
A
Santos-López
Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias-INTIA, Navarra, Spain.
Isidro
I
Lázaro
Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias-INTIA, Navarra, Spain.
Javier
J
Labairu
Hospital Universitari Germans Trias i Pujol, Badalona, Spain Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain.
Cristina
C
Prat
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain Hospital Universitari Bellvitge, Barcelona, Spain.
Carmen
C
Ardanuy
Departamento de Sanidad Animal, Facultad de Veterinaria y VISAVET, Universidad Complutense, Madrid, Spain.
Bruno
B
González-Zorn
Centre de Recerca en Sanitat Animal (CReSA), Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain Virginia.Aragon@creas.uab.cat juncal.garmendia@unavarra.es.
Virginia
V
Aragon
Instituto de Agrobiotecnología, CSIC-Universidad Pública Navarra-Gobierno de Navarra, Mutilva, Spain Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain Virginia.Aragon@creas.uab.cat juncal.garmendia@unavarra.es.
Junkal
J
Garmendia
D000818:Animals; D000831:Animals, Newborn; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D006193:Haemophilus influenzae / Q000235:genetics; D044137:Haemophilus parasuis / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015661:Pasteurellaceae / Q000235:genetics; D010957:Plasmids / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013552:Swine; D014886:Weaning; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases