Whole genome sequencing and phylogenetic analysis of feline anelloviruses.
Torque teno felis virus (FcTTV) was detected in the cat population in the Czech Republic. A total of 110 serum samples were tested by a nested PCR technique using specific primers, situated in the highly conserved untranslated region of the virus genome. The frequency of feline TT virus in the Czech Republic was found to be 33.63%. Sequencing of PCR product from several virus strains showed that all of them are closely related and belong to the same virus species. Whole genome sequencing of three strains was performed to compare overall genetic heterogeneity of feline TT viruses. One of these three strains showed more that 10% difference at the nucleotide level. Furthermore we didn't find any correlation between FcTTV infection and sex or health status of examined animals.
Institute of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Veronika
V
Jarošová
Institute of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Kristýna
K
Hrazdilová
Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Zita
Z
Filipejová
Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Pavel
P
Schánilec
Institute of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. Electronic address: celerv@vfu.cz.
Vladimír
V
Celer
D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000821:virology; D002415:Cats / Q000821:virology; D018153:Czech Republic / Q000453:epidemiology; D004266:DNA Virus Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D022783:Torque teno virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
C446939:ORF1 protein, TT virus; D014764:Viral Proteins
Anelloviruses; Cats; Genome sequencing; Prevalence