Potentiation of ciprofloxacin action against Gram-negative bacterial biofilms by a nitroxide.
We previously showed that soluble nitroxides (nitric oxide analogues) mimicked the well-established ability of nitric oxide to cause biofilm dispersal and further showed that these compounds could prevent biofilm formation. Here, we investigated the effect of the nitroxide carboxy-TEMPO in combination with sub μg/ml concentrations of ciprofloxacin on pre-formed flow cell biofilms formed by Gram-negative bacteria. Combination therapy led to substantial eradication of existing biofilms formed by Pseudomonas aeruginosa PA14 (99.3%) and Escherichia coli O157 (93%).
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Fany
F
Reffuveille
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
César de la
Cde L
Fuente-Núñez
ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Faculty of Science and Engineering, Queensland University of Technology, Queensland 4001, Australia.
Kathryn E
KE
Fairfull-Smith
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada bob@hancocklab.com.
Robert E W
RE
Hancock
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects*; D002939:Ciprofloxacin / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004357:Drug Synergism; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology
D000900:Anti-Bacterial Agents; D003497:Cyclic N-Oxides; D002939:Ciprofloxacin
biofilm; ciprofloxacin; nitroxide