Effects on g2/m phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in l132 nontumorigenic human lung epithelial cells.
The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.
Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.
Hee
H
Jin
Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.
Hye Eun
HE
Yoon
Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 402-751, Korea.
Jae-Seon
JS
Lee
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Korea.
Jae-Kyung
JK
Kim
Power Systems Research Division, Electrical Environment Team, Korea Electrotechnology Research Institute, Changwon 642-120, Korea.
Sung Ho
SH
Myung
Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.
Yun-Sil
YS
Lee
Aneuploidy; Extremely low frequency; G2/M phase; H2O2; Ionizing radiation