Crystal structure of Gib2, a signal-transducing protein scaffold associated with ribosomes in Cryptococcus neoformans.
The atypical Gβ-like/RACK1 Gib2 protein promotes cAMP signalling that plays a central role in regulating the virulence of Cryptococcus neoformans. Gib2 contains a seven-bladed β transducin structure and is emerging as a scaffold protein interconnecting signalling pathways through interactions with various protein partners. Here, we present the crystal structure of Gib2 at a 2.2-Å resolution. The structure allows us to analyse the association between Gib2 and the ribosome, as well as to identify the Gib2 amino acid residues involved in ribosome binding. Our studies not only suggest that Gib2 has a role in protein translation but also present Gib2 as a physical link at the crossroads of various regulatory pathways important for the growth and virulence of C. neoformans.
School of Biological Sciences, Nanyang Technological University, Singapore.
Rya
R
Ero
School of Biological Sciences, Nanyang Technological University, Singapore.
Valya Tenusheva
VT
Dimitrova
School of Biological Sciences, Nanyang Technological University, Singapore.
Yun
Y
Chen
School of Biological Sciences, Nanyang Technological University, Singapore.
Wenting
W
Bu
School of Biological Sciences, Nanyang Technological University, Singapore.
Shu
S
Feng
Public Health Research Institute, Department of Microbiology and Molecular Genetics, Rutgers University, Newark, NJ, USA.
Tongbao
T
Liu
1] The Research Institute for Children, Children's Hospital, New Orleans, LA, USA [2] Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, USA [3] Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
Ping
P
Wang
Public Health Research Institute, Department of Microbiology and Molecular Genetics, Rutgers University, Newark, NJ, USA.
Chaoyang
C
Xue
School of Biological Sciences, Nanyang Technological University, Singapore.
Suet Mien
SM
Tan
1] School of Biological Sciences, Nanyang Technological University, Singapore [2] Institute of Molecular and Cell Biology, A*STAR, Singapore.
Yong-Gui
YG
Gao
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000378:metabolism*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000737:chemistry; D012270:Ribosomes / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D016415:Sequence Alignment; D015398:Signal Transduction
C111825:ASC1 protein, S cerevisiae; D048868:Adaptor Proteins, Signal Transducing; D005656:Fungal Proteins; D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; D039601:Eukaryotic Initiation Factor-4A; D019204:GTP-Binding Proteins