Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage.
This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers inhibit fibril formation. We also investigated cell viability, the production of reactive oxygen species (ROS), and mitochondrial membrane potential. mHippoE-18 cells were preincubated with carbosilane dendrimers before rotenone was added. All the dendrimers possess potential protection activity. Preincubation with dendrimers contributed to: increased viability, higher mitochondrial membrane potential, and reduced ROS level in cells. The probable mechanism of cell protection lies in the ability of dendrimers to capture rotenone by encapsulating or binding to its surface groups. The fact that dendrimers have prevention potential is important in the search for new pharmacological strategies against neurodegenerative disorders.
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland. Electronic address: milowska@biol.uni.lodz.pl.
Katarzyna
K
Milowska
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
Aleksandra
A
Szwed
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
Marta
M
Mutrynowska
Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN).
Rafael
R
Gomez-Ramirez
Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN).
Francisco Javier
FJ
de la Mata
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
Teresa
T
Gabryelak
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
Maria
M
Bryszewska
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D050091:Dendrimers / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*; D012821:Silanes / Q000494:pharmacology*; D051844:alpha-Synuclein / Q000037:antagonists & inhibitors* / Q000378:metabolism
D050091:Dendrimers; D017382:Reactive Oxygen Species; D012821:Silanes; D051844:alpha-Synuclein; C504072:carbosilane; D012402:Rotenone
Carbosilane dendrimers; Parkinson’s disease; Rotenone; α-Synuclein