New insight of squaraine-based biocompatible surface-enhanced Raman scattering nanotag for cancer-cell imaging.
OBJECTIVE
Development of highly sensitive diagnostic nanoprobe for cancer imaging based on surface-enhanced Raman scattering (SERS) platform.


METHODS
Synthesis of novel squaraine dyes as a Raman signature molecule denoted as lipoic-squaraine-lipoic (LSL), propyl-squaraine-lipoic (PSL) and propyl-squaraine-propyl (PSP). The SERS-nanotag constructed with a Raman signature molecule which is attached on gold nanoparticle and further encapsulated with heterofunctionalized PEG. Antibody conjugation with best SERS-nanotag for target specific recognition.


RESULTS
SERS nanotag Au-LSL-PEG showed significant signal intensity and remarkable stability. Anti-EGF receptor and Her2-conjugated Au-LSL-PEG-nanotag were successfully applied for selective recognition of cancer cells like A549, OSCC and MCF7.


CONCLUSIONS
The newly developed SERS-nanotag Au-LSL-PEG serves as a valuable tool for diagnostic detection of cancer cells, and may find potential applications for cancer screening in real patient samples.
CSIR-National Institute for Interdisciplinary Science & Technology, Chemical Science & Technology Division, Organic Chemistry Section, Industrial Estate, Thiruvananthapuram 695019, Kerala, India.
An
A
Ramya
Animesh
A
Samanta
N
N
Nisha
Young-Tae
YT
Chang
Kaustabh Kumar
KK
Maiti
D045744:Cell Line, Tumor; D003503:Cyclobutanes; D006046:Gold; D006801:Humans; D018796:Immunoconjugates; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis*; D010636:Phenols; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
D003503:Cyclobutanes; D006046:Gold; D018796:Immunoconjugates; D010636:Phenols; D018719:Erb-b2 Receptor Tyrosine Kinases; C480596:squaraine
Raman signature molecule; SERS; antibody conjugation; cancer screening; squaraine dye