Three new secoiridoid glycosides from the rhizomes and roots of Gentiana scabra and their anti-inflammatory activities.
Three new (1-3) and 17 known (4-20) iridoid and secoiridoid glycosides were isolated from a methanol extract of the rhizomes and roots of Gentiana scabra. Their chemical structures were elucidated from 1D and 2D NMR, IR absorption, and HR-ESI-MS spectra, as well as comparisons of these data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 6, 10 and 20 exhibited significant inhibitory effects on LPS-induced IL-12 p40 and IL-6 production with IC50 values of 1.62-14.29 μM. Compound 10 also showed a strong inhibitory effect on the LPS-stimulated production of TNF-α with an IC50 value of 10.45 μM.
a School of Biotechnology, Yeungnam University , Gyeongsan , Gyeongbuk 712-749 , South Korea.
Wei
W
Li
Wei
W
Zhou
Sohyun
S
Kim
Jung-Eun
JE
Koo
Yuna
Y
Kim
Young-Sang
YS
Koh
Sang Hee
SH
Shim
Jin Yeul
JY
Ma
Young Ho
YH
Kim
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D030012:Gentiana / Q000737:chemistry*; D053762:Interleukin-12 Subunit p40 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D057889:Iridoid Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D053762:Interleukin-12 Subunit p40; D015850:Interleukin-6; D057889:Iridoid Glycosides; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha
Gentiana scabra; Gentianaceae; anti-inflammatory; secoiridoid glycoside