Haemophilus parasuis induces activation of NF-κB and MAP kinase signaling pathways mediated by toll-like receptors.
Glässer's disease in pigs caused by Haemophilus parasuis is characterized by a severe membrane inflammation. In our previous study, we have identified activation of the transcription factor NF-κB after H. parasuis infection of porcine epithelial cells. In this study, we found that H. parasuis infection also contributed to the activation of p38/JNK MAPK pathway predominantly linked to inflammation, but not the ERK MAPK pathway associated with growth, differentiation and development. Inhibition of NF-κB, p38 and JNK but not ERK activity significantly reduced IL-8 and CCL4 expression by H. parasuis. We also found TLR1, TLR2, TLR4 and TLR6 were required for NF-κB, p38 and JNK MAPK activation. Furthermore, MyD88 and TRIF signaling cascades were essential for H. parasuis-induced NF-κB activation. These results provided new insights into the molecular pathways underlying the inflammatory response induced by H. parasuis.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Yushan
Y
Chen
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Ting
T
Liu
Section of Paediatrics, Imperial College London, St Mary's Campus, London W2 1PG, UK.
Paul
P
Langford
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Kexin
K
Hua
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Shanshan
S
Zhou
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Yajun
Y
Zhai
Hubei Center for Animal Disease Control and Prevention, Wuhan 430070, China.
Hongde
H
Xiao
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Rui
R
Luo
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Dingren
D
Bi
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: jinhui@mail.hzau.edu.cn.
Hui
H
Jin
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Rui
R
Zhou
D000818:Animals; D002460:Cell Line; D054407:Chemokine CCL4 / Q000276:immunology; D006192:Haemophilus Infections / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D048670:MAP Kinase Kinase 4 / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000473:pathology; D051193:Toll-Like Receptors / Q000276:immunology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology
D054407:Chemokine CCL4; D016209:Interleukin-8; D016328:NF-kappa B; D051193:Toll-Like Receptors; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4
Haemophilus parasuis; Inflammatory response; MAPK pathway; NF-κB pathway; TLRs