Cadherin-6B is required for the generation of Islet-1-expressing dorsal interneurons.
Cadherin-6B induces bone morphogenetic protein (BMP) signaling to promote the epithelial mesenchymal transition (EMT) in the neural crest. We have previously found that knockdown of Cadherin-6B inhibits both BMP signaling and the emigration of the early pre-migratory neural crest cells from the dorsal neural tube. In this study, we found that inhibition of BMP signaling in the neural tube, mediated by the ectopic expression of Smad-6 or Noggin, decreased the size of the Islet-1-positive dorsal cell population. Knockdown or loss of function of Cadherin-6B suppressed the generation of Islet-1-expressing cells in the dorsal neural tube, but not the Lim-1/2 positive dorsal cell population. Our results thus indicate that Cadherin-6B is necessary for the generation of Islet-1-positive dorsal interneurons, as well as the initiation of pre-migratory neural crest cell emigration.
East-West Medical Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea; College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: kisookpark@khu.ac.kr.
Ki-Sook
KS
Park
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
Barry M
BM
Gumbiner
D000818:Animals; D030161:Avian Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D015820:Cadherins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002465:Cell Movement; D002642:Chick Embryo; D058750:Epithelial-Mesenchymal Transition; D055785:Gene Knockdown Techniques; D007395:Interneurons / Q000378:metabolism*; D060850:LIM-Homeodomain Proteins / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D054259:Neural Tube / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D015398:Signal Transduction; D051905:Smad6 Protein / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D000099176:Noggin Protein
D030161:Avian Proteins; D019485:Bone Morphogenetic Proteins; D015820:Cadherins; D002352:Carrier Proteins; D060850:LIM-Homeodomain Proteins; D051905:Smad6 Protein; D014157:Transcription Factors; D000099176:Noggin Protein; C063843:insulin gene enhancer binding protein Isl-1; C087310:K cadherin
Bone morphogenetic protein 4; Cadherin-6B; Dorsal interneurons; Islet-1