Effects of neurotrophin receptor-mediated MAGE homology on proliferation and odontoblastic differentiation of mouse dental pulp cells.
OBJECTIVE
This study aimed to investigate effects of neurotrophin receptor-mediated melanoma antigen-encoding gene homology (NRAGE) on proliferation and odontoblastic differentiation of mouse dental pulp cells (mDPCs).


METHODS
Mouse dental pulp cells were infected with recombinant lentivirus to stably knockdown expression of NRAGE, and biological effects of NRAGE on the cells were detected. Proliferation and odontoblastic differentiation of mDPCs were observed. Simultaneously, mRNA and protein levels of NRAGE and nuclear factor-κB (NF-κB) protein expression were detected. Immunofluorescence assay was used to detect expression and location of NRAGE and NF-κB.


RESULTS
NRAGE mRNA and protein levels reduced significantly after mDPC odontoblastic induction. Knockdown of NRAGE inhibited the proliferation of mDPCs. However, knockdown of NRAGE enhanced their odontoblastic differentiation with up-regulated ALPase activity. It also promoted mineral nodule formation as well as mRNA and protein expressions of ALP, DSPP and DMP1. Protein levels of NF-κB/p50 significantly increased, whereas NF-κB/p105 protein expression decreased in the mDPC/shNRG group. Immunofluorescence revealed that relocation of NF-κB was similar to that of NRAGE during odontoblastic induction, in which NF-κB translocated from the cytoplasm to the nucleus.


CONCLUSIONS
NRAGE is a potent regulator of proliferation and odontoblastic differentiation of mDPCs, which might be via the NF-κB signalling pathway.
Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
S
S
Qi
Q
Q
Wu
J
J
Ma
J
J
Li
F
F
Chen
Y
Y
Xu
Q
Q
Pan
R
R
Wang
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D052002:NF-kappa B p50 Subunit / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009804:Odontoblasts / Q000166:cytology*; D010750:Phosphoproteins / Q000096:biosynthesis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D012795:Sialoglycoproteins / Q000096:biosynthesis; D000099311:Dentin Sialophosphoprotein
D048868:Adaptor Proteins, Signal Transducing; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D052002:NF-kappa B p50 Subunit; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C480020:Atrnl1 protein, mouse; C513308:Dmp1 protein, mouse; C486541:Maged1 protein, mouse; C073701:Nfkb1 protein, mouse