Circulating MicroRNA Let-7d in Attention-Deficit/Hyperactivity Disorder.
Up to date, there has been no molecular signature available in the clinical practice for attention-deficit/hyperactivity disorder (ADHD). To investigate circulating miRNA let-7d significance in ADHD, we investigated serum miRNA let-7d in 35 newly diagnosed ADHD subjects who were randomly selected from 406 patients out of 7450 children, paired with gender- and age-matched control through case-control study. We observed that circulating miRNA let-7d was significantly higher in ADHD subjects than in control (p < 0.05). Higher circulation level of miRNA let-7d was significantly associated with ADHD (odds ratio 16.7; 95% confidence, p < 0.05). Meanwhile, serum galectin-3 level was down-regulated in ADHD subjects and the subjects with low galectin-3 expression accounted for 66% in ADHD. The difference of the serum galectin-3 levels between ADHD and non-ADHD groups reached significance (p < 0.05). In 1-year follow-up, a significantly higher rate of clinical improvement was noted in subjects with low level of circulating miRNA let-7d (p < 0.05) than those with high level of circulating miRNA let-7d. Our data demonstrated that miRNA let-7d was elevated in the serum of ADHD subjects, which might be a novel, useful molecule signature for ADHD.
Department of Children's Health Care, Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, 325003, People's Republic of China, jaemny@163.com.
Li Hui
LH
Wu
Min
M
Peng
Mei
M
Yu
Qian Lei
QL
Zhao
Chao
C
Li
Yu Tong
YT
Jin
Yong
Y
Jiang
Zhong Yi
ZY
Chen
Nian Hui
NH
Deng
Hui
H
Sun
Xing Zhong
XZ
Wu
D000293:Adolescent; D000818:Animals; D001289:Attention Deficit Disorder with Hyperactivity / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D004195:Disease Models, Animal; D005260:Female; D037502:Galectin 3 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012494:Sampling Studies; D016896:Treatment Outcome
D015415:Biomarkers; D037502:Galectin 3; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human