TRAF protein function in noncanonical NF-κB signaling.
Nuclear factor-κB (NF-κB) signaling is classified into the canonical and noncanonical pathways. We describe in this chapter the methods used to study the noncanonical pathway, including derivation of primary cells, pathway stimulation, and immunoblotting.
Medical Scientist Training Program, David Geffen School of Medicine at the University of California Los Angeles, 23-385 Center for the Health Sciences, 957041/MC 704117, Los Angeles, CA, 90095, USA.
Anna D
AD
Reichardt
Jose
J
Pindado
Shivam A
SA
Zaver
Genhong
G
Cheng
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D015153:Blotting, Western; D018929:Cell Culture Techniques; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism; D018189:Immunomagnetic Separation / Q000379:methods; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism*; D061251:Primary Cell Culture; D011485:Protein Binding; D021381:Protein Transport; D015398:Signal Transduction; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / Q000378:metabolism*
D016328:NF-kappa B; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins