Pitfalls of insulin-like growth factor-i and growth hormone assays.
Accurate measurement of growth hormone (GH) and insulin-like growth factor-I (IGF-I) are the key to correct diagnosis of acromegaly and GH deficiency. Unfortunately, there is much variation involved when these hormones are measured at different sites and using different assay methods. There is an ongoing global effort to standardize the measurement process to obtain more comparable results in the future. This review discusses common pitfalls in the measurement of GH and IGF-I and guides laboratories in their analyses of these hormones.
Endocrine Research Unit, Medizinische Klinik und Poliklinik IV, Klinikum der Ludwig-Maximilians-Universität (LMU), Ziemssenstr. 1, Munich 80336, Germany.
Riia K
RK
Junnila
Department of Endocrinology and Metabolic Diseases, Campus Charité Mitte, Charité Universitaetsmedizin, Charitéplatz 1, Berlin 10117, Germany.
Christian J
CJ
Strasburger
Endocrine Research Unit, Medizinische Klinik und Poliklinik IV, Klinikum der Ludwig-Maximilians-Universität (LMU), Ziemssenstr. 1, Munich 80336, Germany. Electronic address: martin.bidlingmaier@med.uni-muenchen.de.
Martin
M
Bidlingmaier
D019382:Human Growth Hormone / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D017410:Practice Guidelines as Topic / Q000592:standards*
D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I
Antibody; Binding protein; Growth hormone; Immunoassay; Insulin-like growth factor I; Provocative testing; Standard preparation