Biomineralization of azo dye bearing wastewater in periodic discontinuous batch reactor: Effect of microaerophilic conditions on treatment efficiency.
The present study illustrates the influence of microaerophilic condition on periodic discontinuous batch reactor (PDBR) operation in treating azo dye containing wastewater. The process performance was evaluated with the function of various dye load operations (50-750 mg/l) by keeping the organic load (1.6 kg COD/m(3)-day) constant. Initially, lower dye operation (50mg dye/l) resulted in higher dye [45 mg dye/l (90%)] and COD [SDR: 1.29 kg COD/m(3)-day (92%)] removal efficiencies. Higher dye load operation (750 mg dye/l) also showed non-inhibitory performance with respect to dye [600 mg dye/l (80%)] and COD [1.25 kg COD/m(3)-day (80%)] removal efficiencies. Increment in dye load showed increment in azo reductase and dehydrogenase activities (39.6 U; 4.96 μg/ml; 750 mg/l). UV-Vis spectroscopy (200-800 nm), FTIR and (1)H NMR studies revealed the disappearance of azo bond (-NN-). First derivative cyclic voltammogram supported the involvement of various membrane bound redox shuttlers, viz., cytochrome-C, cytochrome-bc1 and flavoproteins (FAD (H)).
Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500007, India.
A
A
Naresh Kumar
Academy of Scientific and Innovative Research (AcSIR), India.
C
C
Nagendranatha Reddy
Academy of Scientific and Innovative Research (AcSIR), India. Electronic address: vmohan_s@yahoo.com.
S
S
Venkata Mohan
D001391:Azo Compounds / Q000737:chemistry*; D001419:Bacteria / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002384:Catalysis; D004396:Coloring Agents / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D014450:Electron Transport Complex III / Q000737:chemistry; D004583:Electrons; D005420:Flavoproteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry
D001391:Azo Compounds; D004396:Coloring Agents; D045304:Cytochromes c; D014450:Electron Transport Complex III; D005420:Flavoproteins; D007220:Industrial Waste; D010088:Oxidoreductases; D062065:Wastewater; D014874:Water Pollutants, Chemical
Azo dye removal; Azo reductase; Cyclic voltammetry; First derivative analysis; Sequencing batch reactor (SBR)