Microbacterium proteolyticum sp. nov. isolated from roots of Halimione portulacoides.
An endophytic actinobacterial strain RZ36T, isolated from roots of the salt-marsh plant Halimione portulacoides, was subjected to a polyphasic taxonomic characterization. 16S rRNA gene sequence analysis revealed that this strain belonged to the genus Microbacterium. The closest phylogenetic relative was Microbacterium hominis DSM 12509T, with a pairwise 16S rRNA gene sequence similarity of 98.8 %. The DNA-DNA hybridization value between strain RZ36T and M. hominis DSM 12509T was 16 %. The affiliation to the genus Microbacterium was corroborated by phenotypic and chemotaxonomic characteristics. The cell-wall peptidoglycan type was B2β and the diagnostic diamino acid was ornithine. Whole-cell sugars detected were galactose, glucose, rhamnose, ribose and xylose. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0 and the major menaquinone was MK-11 (64 %). Main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The genomic DNA G+C content was 69.7 mol%. Thus, on the basis of phenotypic, genotypic and chemotaxonomic data, strain RZ36T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium proteolyticum sp. nov. (type strain RZ36T = DSM 27100T = CECT 8356T) is proposed.
Departamento de Biologia, CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Artur
A
Alves
Departamento de Microbiología y Genética, Edificio Departamental, Lab 214, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Raúl
R
Riesco
Departamento de Biologia, CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
António
A
Correia
Departamento de Microbiología y Genética, Edificio Departamental, Lab 214, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Martha E
ME
Trujillo
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D027703:Amaranthaceae / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2