Prediction of Pharmacokinetics and Penetration of Moxifloxacin in Human with Intra-Abdominal Infection Based on Extrapolated PBPK Model.
The aim of this study is to develop a physiologically based pharmacokinetic (PBPK) model in intra-abdominal infected rats, and extrapolate it to human to predict moxifloxacin pharmacokinetics profiles in various tissues in intra-abdominal infected human. 12 male rats with intra-abdominal infections, induced by Escherichia coli, received a single dose of 40 mg/kg body weight of moxifloxacin. Blood plasma was collected at 5, 10, 20, 30, 60, 120, 240, 480, 1440 min after drug injection. A PBPK model was developed in rats and extrapolated to human using GastroPlus software. The predictions were assessed by comparing predictions and observations. In the plasma concentration versus time profile of moxifloxcinin rats, Cmax was 11.151 µg/mL at 5 min after the intravenous injection and t1/2 was 2.936 h. Plasma concentration and kinetics in human were predicted and compared with observed datas. Moxifloxacin penetrated and accumulated with high concentrations in redmarrow, lung, skin, heart, liver, kidney, spleen, muscle tissues in human with intra-abdominal infection. The predicted tissue to plasma concentration ratios in abdominal viscera were between 1.1 and 2.2. When rat plasma concentrations were known, extrapolation of a PBPK model was a method to predict drug pharmacokinetics and penetration in human. Moxifloxacin has a good penetration into liver, kidney, spleen, as well as other tissues in intra-abdominal infected human. Close monitoring are necessary when using moxifloxacin due to its high concentration distribution. This pathological model extrapolation may provide reference to the PK/PD study of antibacterial agents.
Tianjin First Central Hospital, Tianjin 300192, China.
LiQin
L
Zhu
Tianjin Medical University, Tianjin 300070, China.
JianWei
J
Yang
Tianjin First Central Hospital, Tianjin 300192, China. ; Tianjin Medical University, Tianjin 300070, China.
Yuan
Y
Zhang
Tianjin Medical University, Tianjin 300070, China.
YongMing
Y
Wang
Tianjin First Central Hospital, Tianjin 300192, China.
JianLei
J
Zhang
The 153 Central Hospital of the Chinese People's Liberation Army, Henan 450000, China.
YuanYuan
Y
Zhao
Tianjin Medical University, Tianjin 300070, China.
WeiLin
W
Dong
Intra-abdominal infection; Moxifloxacin; PBPK modeling; Tissue penetration