Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase.
The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
Laboratory of Bioprocess Engineering, Faculty of Food Engineering - UNICAMP, Campinas, Brazil. Electronic address: susanduarte@hotmail.com.
Susan Hartwig
SH
Duarte
Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Gonzalo Lázaro del Peso
GL
Hernández
Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Albert
A
Canet
Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Maria Dolors
MD
Benaiges
Laboratory of Bioprocess Engineering, Faculty of Food Engineering - UNICAMP, Campinas, Brazil.
Francisco
F
Maugeri
Department of Chemical Engineering, School of Engineering - Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Francisco
F
Valero
D055162:Biocatalysis; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000032:analysis; D006586:Hexanes / Q000737:chemistry; D008049:Lipase / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification; D009821:Oils / Q000737:chemistry*; D000069463:Olive Oil / Q000737:chemistry; D011995:Recombination, Genetic / Q000235:genetics; D012233:Rhizopus / Q000201:enzymology*; D012997:Solvents
D056804:Biofuels; D004800:Enzymes, Immobilized; D004952:Esters; D006586:Hexanes; D008055:Lipids; D009821:Oils; D000069463:Olive Oil; D012997:Solvents; C026385:n-hexane; D008049:Lipase
Biodiesel; Rhizopus oryzae lipase; Single cell oil