Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development.
We achieved possibility of isolation, characterization human umbilical cord blood endothelial progenitor cells (EPCs), examination potency of EPCs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (AMI). EPCs were separated and cultured from umbilical cord blood. Their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated LDL and FITC-labeled Ulex europaeus agglutinin 1 (DILDL-UEA-1). EPCs of cord blood were counted. Human VEGFR-2 and eNOS from the cultured EPCs were assessed by qPCR. Human EPCs was transplanted intramyocardially in canines with AMI. ECG and cardiac enzymes (CK-MB and Troponin I) were measured to assess severity of cellular damage. Histopathology was done to assess neovascularisation. Immunostaining was done to detect EPCs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes (hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted EPCs. Cultured human cord blood exhibited an increased number of EPCs and significant high expression of hVEGFR-2 and eNOS genes in the culture cells. Histopathology showed increased neovascularization and immunostaining showed presence of EPCs newly differentiated into cardiomyocyte-like cells. Our findings suggested that hEPCs can mediate angiogenesis and differentiate into cardiomyoctes in canines with AMI.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Abd El Aziz
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt ; Clinical Biochemistry Department, Faculty of Medicine, King Abdulaziz University, North Jedda, Saudi Arabia.
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Abd El Nabi
Cardiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Abd El Hamid
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Sabry
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt ; Clinical Biochemistry Department, Faculty of Medicine, King Abdulaziz University, Rabigh branch, Jeddah, Saudi Arabia.
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Atta
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Rahed
Surgery Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
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Shamaa
Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Mahfouz
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Taha
Nuclear Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Elrefaay
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
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Gharib
Obstetrics and Gynaecology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Khaled A
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Elsetohy
AMI, acute myocardial infarction; Acute myocardial infarction; CAG, coronary angiography; CFU, colony forming unit; CTO, chronic total occlusion; Canine; DILDL-FITC labeled UEA-11, 1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-labeled acetylated LDL (DiLDL,) and FITC-labeled Ulex europaeus agglutinin-1; Human EPCs; MVD, multivessel disease; Neovascularization