[Preparation and characterization of uricase in uricase-catalase liposomes prepared using borate buffer].
OBJECTIVE
To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer.


METHODS
BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined.


RESULTS
The optimal temperature of BUCLP and URI was 40 degrees celsius, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 µmol/L and 13.623 µmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm.


CONCLUSIONS
Uricase activity is enhanced after loading in uricase and catalase liposomes.
Medicine Engineering Research Center, Key Laboratory of Biochemical & Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China.E-mail: zhouyunli716@163.com.
Yunli
Y
Zhou
Lin
L
Yang
Zijun
Z
Yan
Xue
X
Deng
Jingqing
J
Zhang
D001881:Borates; D002374:Catalase; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry; D013696:Temperature; D014503:Urate Oxidase / Q000737:chemistry*
D001881:Borates; D008081:Liposomes; D002374:Catalase; D014503:Urate Oxidase