Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups.
We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this study. We chose a bacterial strain for polymerase chain reaction (PCR) 25S rDNA genotyping and random amplified polymorphic DNA (RAPD) genotyping. The rate of caries-active in Han children was higher than that in Uygur children, with values of 39.6 and 24.3%, respectively. The detection rate of C. albicans was closely correlated to the caries filling index classification (X(2) = 31.037, P = 0.000, r = 0.421; X(2) = 80.454, P = 0.000, r = 0.497). PCR of 25S rDNA from 40 strains of Han and Uygur children revealed 3 genotypes, while RAPD analysis revealed 5 genotypes. The distribution of 25S rDNA genotyping of Han children from PCR differed from that of Uygur children (X(2) = 7.697, P = 0.021), both of which were mainly the A type. RAPD genotyping of both Han and Uygur children showed similar results (X(2) = 1.573, P = 0.814). There were differences in the distributions of C. albicans in children of different nationalities. C. albicans is a key factor causing caries. The PCR 25S rDNA genotyping method is simple and sensitive, while the RAPD genotyping method is reliable and comprehensive.
Department of Operative Dentistry and Endodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
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N
Wu
Department of Operative Dentistry and Endodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
J
J
Lin
Department of Operative Dentistry and Endodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
L
L
Wu
Department of Operative Dentistry and Endodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China merryljin@sina.com.
J
J
Zhao
D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity; D002675:Child, Preschool; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics*; D003731:Dental Caries / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology / Q000473:pathology; D012335:RNA, Ribosomal / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S