Reduction of protein kinase C α (PKC-α) promote apoptosis via down-regulation of Dicer in bladder cancer.
In clinic, we examined the expression of protein kinase C (PKC)-α and Dicer in the samples of bladder cancer patients, and found that the two proteins have a line correlation. Our study confirmed this correlation existing by clearing the decreasing expression of Dicer after the PKC-α knockdown. Treatment of bladder cancer cell lines (T24, 5637) with the PKC-α or Dicer knockdown and the PKC inhibitors (Calphostin C and Gö 6976) can promote the apoptosis. Inhibition of PKC can increase the activities of caspase-3 and PARP, however, decrease the expression of Dicer. And knockdown of the PKC-α or Dicer can also activate the caspase-3 or the PARP. Considering the reduction of PKC-α can induce the Dicer down-regulation, we make the conclusion that the reduction of PKC-α can promote the apoptosis via the down-regulation of Dicer in bladder cancer.
Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, China.
Zhenming
Z
Jiang
Chuize
C
Kong
Zhe
Z
Zhang
Yuyan
Y
Zhu
Yuxi
Y
Zhang
Xi
X
Chen
D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D015153:Blotting, Western; D002227:Carbazoles / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009281:Naphthalenes / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
D002227:Carbazoles; D004791:Enzyme Inhibitors; D009281:Naphthalenes; C081021:Go 6976; D011065:Poly(ADP-ribose) Polymerases; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha; D043244:Ribonuclease III; D053148:Caspase 3; C058819:calphostin C
Dicer; PKC-α; apoptosis; bladder cancer; caspase-3