Applications of magnetic surface imprinted materials for solid phase extraction of levofloxacin in serum samples.
In this work, molecularly imprinted magnetic carbon nanotubes (MCNTs@MIPs) was prepared with surface imprinting technique for extraction of levofloxacin in serum samples. The preparation of molecularly imprinted polymers (MIPs) used levofloxacin as template, methacrylic acid as functional monomer, and ethylene glycol dimethacrylate as cross-linker, and the magnetic carbon nanotubes (MCNTs) was synthesized by solvothermal method. The prepared polymers not only can be separated and collected easily by an external magnetic, but also exhibited high specific surface area and high selectivity to template molecules. Kinetic adsorption and static adsorption capacity investigations indicated that the synthesized MCNTs@MIPs had excellent recognition towards levofloxacin. Furthermore, magnetic solid phase extraction (MSPE) using the prepared MCNTs@MIPs as sorbent was then investigated, and an efficient sample cleanup was obtained with recoveries ranged from 78.7 ± 4.8 % to 83.4 ± 4.1%. In addition, several parameters, including the pH of samples, the amount of MCNTs@MIPs, the adsorption and desorption times, and the eluent, were investigated to obtain optimal extraction efficiency. Under the optimal extraction conditions, the stability of the polymer was also evaluated, and the average recovery reduced less than 7.6% after 5 cycles. MCNTs@MIPs successfully applied in the preconcentration and determination of levofloxacin in serum sample suggested that the MSPE method based on the novel polymers could be a promising alternative for selective and efficient extraction of trace amounts of pharmaceutical substances in bio-matrix samples.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
Deli
D
Xiao
Cuixia
C
Wang
Hao
H
Dai
Jun
J
Peng
Jia
J
He
Kai
K
Zhang
Sumei
S
Kong
Panzi
P
Qiu
Hua
H
He
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D064704:Levofloxacin / Q000097:blood / Q000302:isolation & purification*; D060328:Magnetic Phenomena; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D052616:Solid Phase Extraction
D000900:Anti-Bacterial Agents; D037742:Nanotubes, Carbon; D011109:Polymethacrylic Acids; D064704:Levofloxacin
carbon nanotubes; levofloxacin; magnetic solid phase extraction; molecularly imprinted polymers; serum samples