The synthesis and characterization of a novel potassium chloride-fluoridated hydroxyapatite varnish for treating dentin hypersensitivity.
Dentin hypersensitivity is treated using materials that occlude the dentinal tubules or release potassium ions that induce nerve desensitization. In this study we formulated a novel varnish containing potassium chloride and fluoridated hydroxyapatite and evaluated its physical properties and cytotoxicity. Potassium ion release from the varnish was measured. Dentin permeability was evaluated by measuring the hydraulic conductance of etched dentin discs treated with the varnish. The direct contact test and MTT assay were performed to evaluate the varnish's cytotoxicity. We found that the varnish released potassium ions over 6 h, and demonstrated a statistically higher reduction in dentin permeability compared to commercial fluoride varnish or control. Dentin disc scanning electron microscopy images demonstrated occluded dentinal tubules in the novel varnish group after brushing. The cytotoxicity tests indicated the varnish was biocompatible with gingival and pulpal fibroblasts. We propose the novel varnish is a potential material for use in hypersensitivity management.
Dental Biomaterials Science, Graduate School-Interdisciplinary Program, Chulalongkorn University.
Yossakit
Y
Lochaiwatana
Suchit
S
Poolthong
Isao
I
Hirata
Masayuki
M
Okazaki
Somporn
S
Swasdison
Noppakun
N
Vongsavan
D003782:Dental Pulp / Q000166:cytology; D057133:Dentin Desensitizing Agents / Q000138:chemical synthesis*; D003806:Dentin Permeability / Q000187:drug effects; D003807:Dentin Sensitivity / Q000188:drug therapy*; D005347:Fibroblasts / Q000187:drug effects; D005460:Fluorides, Topical / Q000737:chemistry; D005881:Gingiva / Q000166:cytology; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011189:Potassium Chloride / Q000737:chemistry*; D012969:Sodium Fluoride / Q000737:chemistry; D018675:Toxicity Tests
D057133:Dentin Desensitizing Agents; D005460:Fluorides, Topical; D006882:Hydroxyapatites; C023807:sodium fluoride topical preparation; C028869:fluor-hydroxylapatite; D011189:Potassium Chloride; D012969:Sodium Fluoride