Control of hydroxyapatite crystal growth by gallic acid.
The purpose of this study is to detect the effect of gallic acid (GA) on hydroxyapatie crystal growth and find the mechanism of the regulation. We evaluated the morphology of HAP crystals grown under various amounts of GA (0, 0.05, 1, and 4 gL(-1)). Subsequently, the chemical composition, crystal size and the morphology were investigated via the energy-dispersive X-ray spectrometer, attenuated total fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction analysis, transmission electron microscope and scanning electron microscopy. In all groups, the Ca/P ratio was closed to 1.67. In the absence of GA, crystals did not arrange, while in the presence of GA, crystals tended to form spherules. The size of the crystals decreased with the concentration of GA increased. These results indicated the role of GA on the growth and morphology of hydroxyapatite crystals, which might be the key mechanism for gallic acid regulating the mineralization.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu; Department of Endodontics and Operative Dentistry, West China Hospital of Stomatology, Sichuan University.
Bei
B
Tang
He
H
Yuan
Lei
L
Cheng
Xuedong
X
Zhou
Xuelian
X
Huang
Jiyao
J
Li
D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry*; D008854:Microscopy, Electron; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D005707:Gallic Acid; D017886:Durapatite