Temporal profile of inflammatory response to fracture and hemorrhagic shock: Proposal of a novel long-term survival murine multiple trauma model.
Hemorrhagic shock (hS) interacts with the posttraumatic immune response and fracture healing in multiple trauma. Due to the lack of a long-term survival multiple trauma animal models, no standardized analysis of fracture healing referring the impact of multiple trauma on fracture healing was performed. We propose a new long-term survival (21 days) murine multiple trauma model combining hS (microsurgical cannulation of carotid artery, withdrawl of blood and continuously blood pressure measurement), femoral (osteotomy/external fixation) and tibial fracture (3-point bending technique/antegrade nail). The posttraumatic immune response was measured via IL-6, sIL-6R ELISA. The hS was investigated via macrohemodynamics, blood gas analysis, wet-dry lung ration and histologic analysis of the shock organs. We proposed a new murine long-term survival (21 days) multiple trauma model mimicking clinical relevant injury patterns and previously published human posttraumatic immune response. Based on blood gas analysis and histologic analysis of shock organs we characterized and standardized our murine multiple trauma model. Furthermore, we revealed hemorrhagic shock as a causative factor that triggers sIL-6R formation underscoring the fundamental pathophysiologic role of the transsignaling mechanism in multiple trauma.
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
Christian
C
Kleber
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
Christopher A
CA
Becker
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
Tom
T
Malysch
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
Jens M
JM
Reinhold
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
Serafeim
S
Tsitsilonis
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
Georg N
GN
Duda
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
Katharina
K
Schmidt-Bleek
Center for Musculoskeletal Surgery and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
Klaus D
KD
Schaser
D000818:Animals; D001784:Blood Gas Analysis; D004195:Disease Models, Animal; D005260:Female; D005264:Femoral Fractures / Q000150:complications / Q000276:immunology*; D017102:Fracture Healing; D006439:Hemodynamics; D015850:Interleukin-6 / Q000097:blood; D008810:Mice, Inbred C57BL; D009104:Multiple Trauma / Q000097:blood / Q000276:immunology*; D020556:Neutrophil Infiltration; D011897:Random Allocation; D019947:Receptors, Interleukin-6 / Q000097:blood; D012119:Respiration; D012771:Shock, Hemorrhagic / Q000097:blood / Q000276:immunology*; D013978:Tibial Fractures / Q000150:complications / Q000276:immunology*; D013997:Time Factors
D015850:Interleukin-6; D019947:Receptors, Interleukin-6
animal model; fracture; hemorrhagic shock; mouse; multiple trauma