Paraoxonase and arylesterase activities in patients with papillary thyroid cancer.
OBJECTIVE
The aim of this study was to evaluate paraoxonase-1 (PON1) activities and oxidative stress status, and the changes in their levels after total thyroidectomy in patients with papillary thyroid cancer (PTC).


METHODS
Twenty-five patients with PTC and 27 healthy controls were enrolled in the study. Blood samples were obtained from the PTC patients before and 3 months after the operation. Preoperative and postoperative serum samples from PTC patients and healthy controls were analyzed for paraoxonase (PON), arylesterase (ARE) activities, and lipid hydroperoxide (LOOH) and -SH (total free sulfhydryl) levels.


RESULTS
The preoperative PON, ARE and -SH levels of the patients with PTC were significantly lower compared to those of the control group (p = 0.033, p < 0.001, p = 0.002, respectively), while LOOH levels were significantly higher (p < 0.001). The levels of PON and ARE decreased significantly in patients with PTC after the operation (p = 0.038, p = 0.023, respectively), while LOOH and -SH levels remained unchanged (p = 0.117, p = 0.487, respectively). PON and ARE levels showed a positive correlation with -SH (r = 0.211, p = 0.065; r = 0.471, p < 0.001, respectively) and a negative correlation with LOOH (r = - 0.391, p < 0.001, r = - 0.486, p < 0.001, respectively).


CONCLUSIONS
Serum PON1 activity is decreased in patients with PTC, and serum PON1 is positively correlated with -SH, a well-known antioxidant, and negatively correlated with LOOH, an oxidant. PON1 activity is significantly decreased after total thyroidectomy.
Department of Internal Medicine, Division of Endocrinology, Faculty of Medicine, Gaziantep University , Gaziantep , Turkey.
Hakan
H
Korkmaz
Suzan
S
Tabur
Mesut
M
Özkaya
Nurten
N
Aksoy
Hamit
H
Yildiz
Ersin
E
Akarsu
D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D002291:Carcinoma, Papillary / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D018384:Oxidative Stress; D013438:Sulfhydryl Compounds / Q000097:blood; D013964:Thyroid Neoplasms / Q000097:blood / Q000201:enzymology* / Q000473:pathology
D008055:Lipids; D013438:Sulfhydryl Compounds; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
Arylesterase; oxidative status; papillary thyroid cancer; paraoxonase