Manipulation of a quasi-natural cell block for high-efficiency transplantation of adherent somatic cells.
Recent advances have raised hope that transplantation of adherent somatic cells could provide dramatic new therapies for various diseases. However, current methods for transplanting adherent somatic cells are not efficient enough for therapeutic applications. Here, we report the development of a novel method to generate quasi-natural cell blocks for high-efficiency transplantation of adherent somatic cells. The blocks were created by providing a unique environment in which cultured cells generated their own extracellular matrix. Initially, stromal cells isolated from mice were expanded in vitro in liquid cell culture medium followed by transferring the cells into a hydrogel shell. After incubation for 1 day with mechanical agitation, the encapsulated cell mass was perforated with a thin needle and then incubated for an additional 6 days to form a quasi-natural cell block. Allograft transplantation of the cell block into C57BL/6 mice resulted in perfect adaptation of the allograft and complete integration into the tissue of the recipient. This method could be widely applied for repairing damaged cells or tissues, stem cell transplantation, ex vivo gene therapy, or plastic surgery.
Department of Biomedical Sciences, Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Chonbuk, South Korea.
H J
HJ
Chung
Department of Biomedical Sciences, Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Chonbuk, South Korea.
M M
MM
Hassan
Department of Biomedical Sciences, Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Chonbuk, South Korea.
J O
JO
Park
JINIS BDRD Institute, JINIS Biopharmaceuticals Co., Wanju, Chonbuk, South Korea.
H J
HJ
Kim
Department of Biomedical Sciences, Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Chonbuk, South Korea.
S T
ST
Hong
D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000637:transplantation*; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D003094:Collagen; D004338:Drug Combinations; D004526:Efficiency; D005260:Female; D007797:Laminin; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008810:Mice, Inbred C57BL; D061251:Primary Cell Culture / Q000379:methods; D011509:Proteoglycans; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous / Q000379:methods
D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen