Short communication: Variable number of tandem repeat polymorphisms in DGAT1 gene of buffaloes (Bubalus bubalis) is associated with milk constituents.
The diacylglycerol-O-transferase 1 gene is a positional and functional candidate for milk composition traits. The objective of this study was to evaluate the segregation of the variable number of tandem repeat polymorphisms in the regulatory region of diacylglycerol-O-transferase 1 gene in a water buffalo herd, and to assess the association of this mutation with milk production traits. For this purpose, 196 Murrah buffalo cows were genotyped by PCR. The association of the marker with total milk, fat, and protein yields at 305 d of lactation, milk fat and protein percentage, and somatic cell scores were evaluated by single-trait analyses using a generalized mixed model. Two segregating alleles were identified in the population. The allele with 2 repeats affected fat percentage favorably. The present results suggest that this polymorphism is an interesting marker to include in the genetic evaluation of buffaloes.
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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Cardoso
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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de Souza
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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Aspilcueta-Borquis
Instituto Federal Goiano-Campus Rio Verde, GO 75901-970, Rio Verde, Brazil.
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Araujo Neto
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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de Camargo
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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Hurtado-Lugo
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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Scalez
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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de Freitas
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil.
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Albuquerque
Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, SP 14884-900, Brazil. Electronic address: tonhati@fcav.unesp.br.
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Tonhati
D000483:Alleles; D000818:Animals; D002020:Buffaloes / Q000235:genetics; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D004041:Dietary Fats / Q000032:analysis; D005260:Female; D005838:Genotype; D007774:Lactation; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D018598:Minisatellite Repeats; D009154:Mutation; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
D004041:Dietary Fats; D008894:Milk Proteins; D051048:Diacylglycerol O-Acyltransferase
Murrah; fat percentage; molecular marker; variable number of tandem repeats (VNTR)