It takes acid, rather than ice, to freeze glucose.
Plasma glucose levels provide the cornerstone of diabetes evaluation. Unfortunately, glucose levels drop in vitro due to glycolysis. Guidelines provide suitable conditions which minimize glycolysis, such as immediate centrifugation or the use of ice/water slurry storage containers. For obvious practical reasons, most laboratories use blood collection tubes containing glycolysis inhibitors. We describe the effect of a variety of commonly used blood collection tubes on in vitro stability of glucose. Furthermore, we looked at the validity of the assumption that glycolytic activity is minimal when blood is kept in an ice/water slurry. Sodium fluoride alone does not reduce in vitro glycolysis in the first 120 minutes after phlebotomy. Addition of citrate almost completely prevented in vitro glycolysis, but showed a positive bias (0.2 mmol/l) compared to control. This is partly due to a small drop in glucose level in control blood, drawn according to the current guidelines. This drop occurs within 15 minutes, in which glycolysis has been described to be minimal and acceptable. NaF-EDTA-citrate based test tubes provide the best pre-analytical condition available. Furthermore, glucose levels are not stable in heparinized blood placed in an ice/water slurry. We strongly advise the use of NaF-EDTA-citrate based test tubes in diabetes research.
Dept. of Clinical Chemistry and Hematology, Amphia Hospital, Breda, The Netherlands.
S A A
SA
van den Berg
Dept. of Clinical Chemistry and Hematology, Amphia Hospital, Breda, The Netherlands.
M H M
MH
Thelen
Dept. of Clinical Chemistry and Hematology, Amphia Hospital, Breda, The Netherlands.
L P W
LP
Salden
Dept. of Internal Medicine, Amphia Hospital, Breda, The Netherlands.
S W
SW
van Thiel
Dept. of Clinical Chemistry and Hematology, Amphia Hospital, Breda, The Netherlands.
K J M
KJ
Boonen
D016477:Artifacts; D001786:Blood Glucose / Q000032:analysis / Q000737:chemistry*; D001800:Blood Specimen Collection / Q000379:methods*; D002951:Citrates / Q000737:chemistry*; D005260:Female; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007053:Ice; D008297:Male; D012969:Sodium Fluoride / Q000737:chemistry*
D001786:Blood Glucose; D002951:Citrates; D007053:Ice; D012969:Sodium Fluoride