A Microfluidic Diffusion Cell for Fast and Easy Percutaneous Absorption Assays.
OBJECTIVE
Percutaneous absorption assays of molecules for pharmaceutical and cosmetology purposes are important to determine the bioavailability of new compounds, once topically applied. The current method of choice is to measure the rate of diffusion through excised human skin using a diffusion cell. This method however entails significant drawbacks such as scarce availability and poor reproducibility of the sample, low sampling rate, and tedious assay setup.


METHODS
The objective of the present work is to propose an alternative method that overcomes these issues by integrating an experimental model of the skin (artificial stratum corneum) and online optical sensors into a microfluidic device.


RESULTS
The measurement of the diffusion profile followed by the calculation of the permeability coefficients and time lag were performed on seven different molecules and obtained data positively fit with those available from literature on human skin penetration. The coating of the lipid mixture to generate the artificial stratum corneum also proved robust and reproducible. The results show that the proposed device is able to give fast, real-time, accurate, and reproducible data in a user-friendly manner, and can be produced at a large scale.


CONCLUSIONS
These assets should help both the cosmetics and pharmaceutics fields where the skin is the target or a pathway of a formulated compound, by allowing more candidate molecules or formulations to be assessed during the various stages of their development.
LIMMS/CNRS-IIS (UMI 2820), Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, 153-8505, Tokyo, Japan, cprovin@iis.u-tokyo.ac.jp.
Christophe
C
Provin
Alexandre
A
Nicolas
Sébastien
S
Grégoire
Teruo
T
Fujii
D000279:Administration, Cutaneous; D002463:Cell Membrane Permeability; D002626:Chemistry, Pharmaceutical; D003199:Computer Systems; D003358:Cosmetics / Q000493:pharmacokinetics; D003517:Cyclopentanes / Q000493:pharmacokinetics; D004058:Diffusion; D015194:Diffusion Chambers, Culture; D006801:Humans; D066298:In Vitro Techniques; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D054883:Oxylipins / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012869:Skin Absorption
D003358:Cosmetics; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid