Comparison of the performance of 2 commercial multiplex PCR platforms for detection of respiratory viruses in upper and lower tract respiratory specimens.
The performance of the CLART® PneumoVir system with that of the Luminex xTAG RVP Fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n=183) from unique patients with influenza-like syndrome or lower tract respiratory infection. Nested PCR coupled to automated sequencing was used for resolution of discrepancies. Fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n=21) or fully discordant (n=35) results (Kappa coefficient, 0.62). The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, influenza A virus/H3N2, influenza B virus, human metapneumovirus, and parainfluenza virus, whereas the sensitivity of the latter was higher for most targeted viruses except, notably, for picornaviruses. This was irrespective of either the origin of the respiratory specimen or the age group to which the patients belonged.
Servicio de Microbiología, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
Elisa
E
Costa
Servicio de Microbiología, Hospital Ramón y Cajal, and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain; Red Española para la Investigación en Enfermedades Infecciosas (REIPI), Madrid, Spain.
Mario
M
Rodríguez-Domínguez
Servicio de Microbiología, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
María Ángeles
MÁ
Clari
Servicio de Microbiología, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
Estela
E
Giménez
Servicio de Microbiología, Hospital Ramón y Cajal, and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain; CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
Juan Carlos
JC
Galán
Servicio de Microbiología, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain. Electronic address: david.navarro@uv.es.
David
D
Navarro
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012137:Respiratory System / Q000821:virology*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D014780:Viruses / Q000302:isolation & purification*; D055815:Young Adult
Acute respiratory tract infection; Multiplex PCR; Respiratory viruses