Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress.
Ascorbate (AsA)-glutathione (GSH) cycle metabolism has been regarded as the most important defense mechanism for the resistance of plants under stress. In this study the influence of salicylic acid (SA) was studied on ascorbate-glutathione pathway, S-assimilation, photosynthesis and growth of mustard (Brassica juncea L.) plants subjected to 100 mM NaCl. Treatment of SA (0.5 mM) alleviated the negative effects of salt stress and improved photosynthesis and growth through increase in enzymes of ascorbate-glutathione pathway which suggest that SA may participate in the redox balance under salt stress. The increase in leaf sulfur content through higher activity of ATP sulfurylase (ATPS) and serine acetyl transferase (SAT) by SA application was associated with the increased accumulation of glutathione (GSH) and lower levels of oxidative stress. These effects of SA were substantiated by the findings that application of SA-analog, 2,6, dichloro-isonicotinic acid (INA) and 1 mM GSH treatment produced similar results on rubisco, photosynthesis and growth of plants establishing that SA application alleviates the salt-induced decrease in photosynthesis mainly through inducing the enzyme activity of ascorbate-glutathione pathway and increased GSH production. Thus, SA/GSH could be a promising tool for alleviation of salt stress in mustard plants.
a Department of Botany; Jamia Hamdard University ; New Delhi , India.
Rahat
R
Nazar
Shahid
S
Umar
Nafees A
NA
Khan
D001205:Ascorbic Acid / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D009149:Mustard Plant / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects*; D010937:Plant Growth Regulators / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology*; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000009:adverse effects; D013455:Sulfur / Q000378:metabolism*
D010937:Plant Growth Regulators; D012965:Sodium Chloride; D013455:Sulfur; D005978:Glutathione; D020156:Salicylic Acid; D001205:Ascorbic Acid
APX, Ascorbate peroxidase; ATP-sulfurylase; ATPS, ATP-sulfurylase; AsA-GSH, Ascorbate-glutathione; CAT, Catalase; Cys, Cysteine; DAS, Days after sowing; DHA, Dehydroascorbate; DHAR, Dehydroascorbate reductase; Fv/Fm, maximal PS II photochemical efficiency; GR, Glutathione reductase; GSH, Reduced glutathione; GSSG, Oxidized glutathione; ROS, Reactive oxygen species; RuBP, ribulose 1, 5-bisphosphate; S, sulfur; SAT, Serine acetyl transferase; TBARS, Thiobarbituric acid reactive substances; WUE, water use efficiency.; ascorbate; glutathione; gs, stomatal conductance; oxidative stress; photosynthesis; salicylic acid; salt stress