Proteomic and gene expression analysis of zebrafish brain undergoing continuous light/dark stress.
Several organisms irrespective of their complexity in structure and function have an inbuilt circadian rhythm. Zebrafish could be used as an alternate model animal in sleep research as it exhibits similar sleep-wake dynamics as mammals and Drosophila. In this study, we have analysed the adult zebrafish brain for its differential proteome and gene expression during perturbed light/dark cycle. A total of 53 and 25 proteins including sncb, peroxiredoxins and TCR alpha were identified based on two-dimensional gel electrophoresis Fourier transform mass spectrometer/ion trap tandem mass spectrometer and differential in-gel electrophoresis MALDI TOF MS/MS analysis, respectively, with at least 1.5-fold changes between the control and experimental brains. Real time-polymerase chain reaction revealed that many circadian pathway-associated genes, such as per1b, bmal1b, cry1b, bmal2 and nr1d2, were differentially regulated during continuous light/dark exposures. It is hypothesized that the differential regulation of these genes might lead to the discovery of potential diagnostic markers for gaining insight into the light/dark-associated stress in humans.
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Sruthi
S
Purushothaman
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Sandeep
S
Saxena
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Vupalapathy
V
Meghah
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Mula G
MG
Meena Lakshmi
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Sachin K
SK
Singh
Proteomics Facility, CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Cherukuvada V
CV
Brahmendra Swamy
CSIR - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India.
Mohammed M
MM
Idris
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects*; D002940:Circadian Rhythm / Q000235:genetics / Q000528:radiation effects*; D003624:Darkness; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008027:Light; D008297:Male; D023421:Models, Animal; D017440:Photoperiod; D020543:Proteome / Q000378:metabolism / Q000528:radiation effects*; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D012890:Sleep / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological / Q000528:radiation effects*; D053719:Tandem Mass Spectrometry; D014851:Wakefulness / Q000528:radiation effects; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000378:metabolism*
D020543:Proteome; D029961:Zebrafish Proteins
2DE; DIGE; Network Pathway