A lectin S-domain receptor kinase mediates lipopolysaccharide sensing in Arabidopsis thaliana.
The sensing of microbe-associated molecular patterns (MAMPs) triggers innate immunity in animals and plants. Lipopolysaccharide (LPS) from Gram-negative bacteria is a potent MAMP for mammals, with the lipid A moiety activating proinflammatory responses via Toll-like receptor 4 (TLR4). Here we found that the plant Arabidopsis thaliana specifically sensed LPS of Pseudomonas and Xanthomonas. We isolated LPS-insensitive mutants defective in the bulb-type lectin S-domain-1 receptor-like kinase LORE (SD1-29), which were hypersusceptible to infection with Pseudomonas syringae. Targeted chemical degradation of LPS from Pseudomonas species suggested that LORE detected mainly the lipid A moiety of LPS. LORE conferred sensitivity to LPS onto tobacco after transient expression, which demonstrated a key function in LPS sensing and indicated the possibility of engineering resistance to bacteria in crop species.
1] Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany. [2] Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
Stefanie
S
Ranf
Division of Immunochemistry/Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.
Nicolas
N
Gisch
Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
Milena
M
Schäffer
Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
Tina
T
Illig
Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
Lore
L
Westphal
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Yuriy A
YA
Knirel
Division of Immunochemistry/Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.
Patricia M
0000000310617023
PM
Sánchez-Carballo
Division of Immunochemistry/Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.
Ulrich
U
Zähringer
Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
Ralph
R
Hückelhoven
Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
Justin
0000000182697494
J
Lee
Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
Dierk
D
Scheel
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology*; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D057865:Plant Immunity / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology*; D044224:Pseudomonas syringae / Q000737:chemistry / Q000276:immunology; D015398:Signal Transduction; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D019076:Transgenes; D016959:Xanthomonas campestris / Q000737:chemistry / Q000276:immunology
D029681:Arabidopsis Proteins; D008070:Lipopolysaccharides; D017346:Protein Serine-Threonine Kinases; C000597754:SD1-29 protein, Arabidopsis