Serum containing Tongqiaohuoxue decoction suppresses glutamate-induced PC12 cell injury.
Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctorius, Rhizoma chuanxiong, Semen pruni persicae, and Radix Paeoniae Rubra. After 24 hours of co-cultivation, glutamate (12.5 mM) was added to the culture medium. We found that serum containing Tongqiaohuoxue decoction prevented the increase in reactive oxygen species, and the decreases in superoxide dismutase and Na(+)-K(+)-ATPase activity, induced by glutamate. It also reduced the concentration of malondialdehyde, enhanced the mitochondrial transmembrane potential, inhibited the elevation of cellular calcium, and decreased phosphorylation of calmodulin-dependent protein kinase II. Thus, serum containing Tongqiaohuoxue decoction had protective effects on cell proliferation and membrane permeability in glutamate-injured PC12 cells.
Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032, Anhui Province, China ; Anhui Anke Biotechnology (Group) Co., Ltd., Hefei 230088, Anhui Province, China ; Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China.
Ning
N
Wang
Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China.
Yi
Y
Deng
Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032, Anhui Province, China.
Wei
W
Wei
Anhui Anke Biotechnology (Group) Co., Ltd., Hefei 230088, Anhui Province, China.
Lihua
L
Song
Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China.
Yan
Y
Wang
PC12 cells; Tongqiaohuoxue decoction; glutamate; neural regeneration; serum pharmacology of Chinese medicine