Collagen matrix as a tool in studying fibroblastic cell behavior.
Type I collagen is a fibrillar protein, a member of a large family of collagen proteins. It is present in most body tissues, usually in combination with other collagens and other components of extracellular matrix. Its synthesis is increased in various pathological situations, in healing wounds, in fibrotic tissues and in many tumors. After extraction from collagen-rich tissues it is widely used in studies of cell behavior, especially those of fibroblasts and myofibroblasts. Cells cultured in a classical way, on planar plastic dishes, lack the third dimension that is characteristic of body tissues. Collagen I forms gel at neutral pH and may become a basis of a 3D matrix that better mimics conditions in tissue than plastic dishes.
a Department of Medical Biochemistry; Medical Faculty in Hradec Králové; Charles University ; Prague , Czech Republic.
Jiří
J
Kanta
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006801:Humans
D003094:Collagen
3-dimensional; TGFβ-1; AP-1; activator protein 1; ECM; c-Jun N-terminal kinase; MFB; cell culture; cell proliferation; collagen; extracellular matrix; extracellular matrix; ERK; extracellular signal-regulated kinase; FAK; fibroblasts; fibronectin; focal adhesion kinase; GT; granulation tissue; HSC; hepatic stellate cells; JNK; integrins; megakaryoblastic leukemia 1; MMP; metalloproteinases; metalloproteinases; NF-κB; myofibroblasts; myofibroblasts; MKL1; nuclear factor kappa B; PI3K/Akt; phosphatidylinositide 3-kinase/Ak strain transforming; PEG; polyethylene glycol; α-SMA; substrate stiffness; tissue inhibitor of metalloproteinases; TNF-α; transforming growth factor β 1; TIMP; tumor necrosis factor α; α-smooth muscle actin; 3D