Construction and use of a zebrafish heart voltage and calcium optical mapping system, with integrated electrocardiogram and programmable electrical stimulation.
Zebrafish are increasingly being used as a model of vertebrate cardiology due to mammalian-like cardiac properties in many respects. The size and fecundity of zebrafish make them suitable for large-scale genetic and pharmacological screening. In larger mammalian hearts, optical mapping is often used to investigate the interplay between voltage and calcium dynamics and to investigate their respective roles in arrhythmogenesis. This report outlines the construction of an optical mapping system for use with zebrafish hearts, using the voltage-sensitive dye RH 237 and the calcium indicator dye Rhod-2 using two industrial-level CCD cameras. With the use of economical cameras and a common 532-nm diode laser for excitation, the rate dependence of voltage and calcium dynamics within the atrial and ventricular compartments can be simultaneously determined. At 140 beats/min, the atrial action potential duration was 36 ms and the transient duration was 53 ms. With the use of a programmable electrical stimulator, a shallow rate dependence of 3 and 4 ms per 100 beats/min was observed, respectively. In the ventricle the action potential duration was 109 ms and the transient duration was 124 ms, with a steeper rate dependence of 12 and 16 ms per 100 beats/min. Synchronous electrocardiograms and optical mapping recordings were recorded, in which the P-wave aligns with the atrial voltage peak and R-wave aligns with the ventricular peak. A simple optical pathway and imaging chamber are detailed along with schematics for the in-house construction of the electrocardiogram amplifier and electrical stimulator. Laboratory procedures necessary for zebrafish heart isolation, cannulation, and loading are also presented.
Molecular Cardiac Physiology Group, Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada;
Eric
E
Lin
Molecular Cardiac Physiology Group, Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada;
Calvin
C
Craig
Molecular Cardiac Physiology Group, Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada;
Marcel
M
Lamothe
Biomedical Optics Research Group, School of Engineering Science, Simon Fraser University, Burnaby;
Marinko V
MV
Sarunic
Medical Image Analysis Lab, School of Engineering Science, Simon Fraser University, Burnaby, Canada; and.
Mirza Faisal
MF
Beg
Molecular Cardiac Physiology Group, Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada; Cardiovascular Sciences, Child and Family Research Institute, Vancouver, Canada tibbits@sfu.ca.
Glen F
GF
Tibbits
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D016275:Atrial Function / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D004558:Electric Stimulation; D004562:Electrocardiography; D022062:Electrophysiologic Techniques, Cardiac; D055724:Electrophysiological Phenomena; D006321:Heart / Q000294:innervation* / Q000502:physiology; D016276:Ventricular Function / Q000502:physiology; D056969:Voltage-Sensitive Dye Imaging; D015027:Zebrafish / Q000502:physiology*
D002118:Calcium
RH 237; Rhod-2; action potential; calcium dynamics; teleost heart