Mouse fibroblast L929 cells are less permissive to infection by Nelson Bay orthoreovirus compared to other mammalian cell lines.
In recent years, bats have been identified as a natural reservoir for a diverse range of viruses. Nelson Bay orthoreovirus (NBV) was first isolated from the heart blood of a fruit bat (Pteropus poliocephalus) in 1968. While the pathogenesis of NBV remains unknown, other related members of this group have caused acute respiratory disease in humans. Thus the potential for NBV to impact human health appears plausible. Here, to increase our knowledge of NBV, we examined the replication and infectivity of NBV using different mammalian cell lines derived from bat, human, mouse and monkey. All cell lines supported the replication of NBV; however, L929 cells showed a greater than 2 log reduction in virus titre compared with the other cell lines. Furthermore, NBV did not induce major cytopathic effects in the L929 cells, as was observed in other cell lines. Interestingly, the related Pteropine orthoreoviruses, Pulau virus (PulV) and Melaka virus (MelV) were able to replicate to high titres in L929 cells but infection resulted in reduced cytopathic effect. Our study demonstrates a unique virus-host interaction between NBV and L929 cells, where cells effectively control viral infection/replication and limit the formation of syncytia. By elucidating the molecular mechanisms that control this unique relationship, important insights will be made into the biology of this fusogenic virus.
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia 2ARC Centre of Excellence in Plant Cell Walls, The University of Melbourne, Parkville, Victoria 3010, Australia.
Lawrence
L
Mok
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
James W
JW
Wynne
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Samantha
S
Grimley
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Brian
B
Shiell
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Diane
D
Green
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Paul
P
Monaghan
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Jackie
J
Pallister
2ARC Centre of Excellence in Plant Cell Walls, The University of Melbourne, Parkville, Victoria 3010, Australia.
Antony
A
Bacic
1CSIRO Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia.
Wojtek P
WP
Michalski
D000818:Animals; D002460:Cell Line / Q000821:virology*; D002685:Chiroptera; D005347:Fibroblasts / Q000821:virology*; D000882:Haplorhini; D006801:Humans; D051379:Mice; D018165:Orthoreovirus / Q000254:growth & development / Q000502:physiology*; D019562:Viral Load; D056189:Viral Tropism; D014776:Virus Cultivation; D014779:Virus Replication