Assay methods for H2S biogenesis and catabolism enzymes.
H2S is produced from sulfur-containing amino acids, cysteine and homocysteine, or a catabolite, 3-mercaptopyruvate, by three known enzymes: cystathionine β-synthase, γ-cystathionase, and 3-mercaptopyruvate sulfurtransferase. Of these, the first two enzymes reside in the cytoplasm and comprise the transsulfuration pathway, while the third enzyme is found both in the cytoplasm and in the mitochondrion. The following mitochondrial enzymes oxidize H2S: sulfide quinone oxidoreductase, sulfur dioxygenase, rhodanese, and sulfite oxidase. The products of the sulfide oxidation pathway are thiosulfate and sulfate. Assays for enzymes involved in the production and oxidative clearance of sulfide to thiosulfate are described in this chapter.
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA. Electronic address: rbanerje@umich.edu.
Ruma
R
Banerjee
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Taurai
T
Chiku
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Omer
O
Kabil
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Marouane
M
Libiad
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Nicole
N
Motl
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Pramod K
PK
Yadav
D000818:Animals; D003541:Cystathionine beta-Synthase / Q000737:chemistry*; D003542:Cystathionine gamma-Lyase / Q000737:chemistry*; D049308:Dioxygenases / Q000737:chemistry; D057075:Enzyme Assays; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D013466:Sulfurtransferases / Q000737:chemistry; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry
D049308:Dioxygenases; C477654:sulfur dioxygenase; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
Cystathionine β-synthase; Hydrogen sulfide; Mercaptopyruvate sulfurtransferase; Rhodanese; Sulfur dioxygenase; γ-Cystathionase