Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression.
Eukaryotic translation elongation factor 1 alpha (EEF1A) plays a key role in protein synthesis. In higher vertebrates EEF1A occurs in two isoforms, EEF1A1 and EEF1A2, encoded by distinct genes. The purpose of this study was to compare the two porcine genes as for the genomic sequence, gene organization and mRNA expression in different tissues, as well as to search for polymorphism and chromosomal assignment. Standard methods of DNA and mRNA analysis were used. We determined the complete genomic sequence of the porcine EEF1A1 and EEF1A2 genes. The two genes differ in the lengths of transcription units (3102 and 8588 bp, respectively), but have similar genomic organization and their coding sequences are highly similar (78% identity of coding sequences and 92.4% identity of amino acid sequences). Several polymorphisms in the two genes were detected. EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively. mRNA of EEF1A1 was expressed in all studied tissues (the highest expression was in 44-day fetal muscle and low expression in adult liver and brain), while EEF1A2 was expressed only in skeletal-muscle, tongue, heart, diaphragm and brain tissues. EEF1A2 was not expressed in fetal muscle tissue (44 days). In this paper results are provided on genomic sequences, genomic organization, polymorphism, chromosomal assignment and spatial and temporal expressions of the porcine EEF1A1 and EEF1A2 genes. Novel polymorphisms were described in both genes. Porcine EEF1A2 was studied for the first time.
Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic.
Kateřina
K
Svobodová
Pavel
P
Horák
Antonín
A
Stratil
Heinz
H
Bartenschlager
Mario
M
Van Poucke
Pavla
P
Chalupová
Věra
V
Dvořáková
Christoph
C
Knorr
Roman
R
Stupka
Jaroslav
J
Čítek
Michal
M
Šprysl
Anna
A
Palánová
Luc J
LJ
Peelman
Hermann
H
Geldermann
Aleš
A
Knoll
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D020869:Gene Expression Profiling; D023281:Genomics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D020652:Peptide Elongation Factor 2 / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
D020648:Peptide Elongation Factor 1; D020652:Peptide Elongation Factor 2