DNA base pair resolution measurements using resonance energy transfer efficiency in lanthanide doped nanoparticles.
Lanthanide-doped nanoparticles are of considerable interest for biodetection and bioimaging techniques thanks to their unique chemical and optical properties. As a sensitive luminescence material, they can be used as (bio) probes in Förster Resonance Energy Transfer (FRET) where trivalent lanthanide ions (La3+) act as energy donors. In this paper we present an efficient method to transfer ultrasmall (ca. 8 nm) NaYF4 nanoparticles dispersed in organic solvent to an aqueous solution via oxidation of the oleic acid ligand. Nanoparticles were then functionalized with single strand DNA oligomers (ssDNA) by inducing covalent bonds between surface carboxylic groups and a 5' amine modified-ssDNA. Hybridization with the 5' fluorophore (Cy5) modified complementary ssDNA strand demonstrated the specificity of binding and allowed the fine control over the distance between Eu3+ ions doped nanoparticle and the fluorophore by varying the number of the dsDNA base pairs. First, our results confirmed nonradiative resonance energy transfer and demonstrate the dependence of its efficiency on the distance between the donor (Eu3+) and the acceptor (Cy5) with sensitivity at a nanometre scale.
LBPA, IDA (FR3242), ENS Cachan, 61 Avenue du Président Wilson, F-94235 Cachan, France.
Aleksandra
A
Delplanque
Institute of Physical and Theoretical Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Dominika
D
Wawrzynczyk
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland.
Pawel
P
Jaworski
Institute of Physical and Theoretical Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Katarzyna
K
Matczyszyn
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland; Department of Toxicology, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.
Krzysztof
K
Pawlik
LBPA, IDA (FR3242), ENS Cachan, 61 Avenue du Président Wilson, F-94235 Cachan, France.
Malcolm
M
Buckle
Institute of Physical and Theoretical Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Marcin
M
Nyk
LBPA, IDA (FR3242), ENS Cachan, 61 Avenue du Président Wilson, F-94235 Cachan, France.
Claude
C
Nogues
Institute of Physical and Theoretical Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Marek
M
Samoc
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004735:Energy Transfer; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles
D028581:Lanthanoid Series Elements; D004247:DNA