Extremophilic polysaccharide nanoparticles for cancer nanotherapy and evaluation of antioxidant properties.
Polysaccharides that show finest bioactivities and physicochemical properties are always promising for bionanoscience applications. Mauran is such a macromolecule extracted from halophilic bacterium, Halomonas maura for biotechnology and nanoscience applications. Antioxidant properties of MR/CH nanoparticles were studied using biochemical assays to prove the versatility of these test nanoparticles for biomedical applications. Here, we demonstrate the prospects of extremophilic polysaccharide, mauran based nanoparticles for scavenging reactive oxygen species in both in vitro and ex vivo conditions. 5-fluorouracil loaded MR/CH nanoparticles were tested for anticancer proliferation and compared their therapeutic efficiency using breast adenocarcinoma and glioma cells. Fluorescently labeled nanoparticles were employed to show the cellular uptake of these nanocarriers using confocal microscopic imaging and flow cytometry.
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Sreejith
S
Raveendran
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Vivekanandan
V
Palaninathan
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Yutaka
Y
Nagaoka
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Takahiro
T
Fukuda
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Seiki
S
Iwai
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Toshiaki
T
Higashi
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Toru
T
Mizuki
Saitama Medical University, Biomedical Research Centre, Division of Analytical Science, Saitama, 350-0495 Japan.
Yasushi
Y
Sakamoto
Sree Chitra Tirunal Institute for Medical Sciences and Technology (Govt. of India), Toxicology Division, BMT Wing, Thiruvananthapuram 695 012, Kerala, India.
P V
PV
Mohanan
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
Toru
T
Maekawa
Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan. Electronic address: sakthi@toyo.jp.
D Sakthi
DS
Kumar
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020632:Halomonas / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides
Antioxidant mechanism; Cancer therapy; Extremophiles; Mauran; Nanoparticles; Polysaccharides