Oxidative stress markers at birth: Analyses of a neonatal population.
In order to further understand neonatal stress and, thus, control it efficaciously, there is a need for more information on the manifestations of stress at the molecular level in the newborn, with particular regard to oxidants, and anti-oxidant and anti-stress mechanisms, including mitochondrial heat shock protein-chaperones such as Hsp60. We investigated patterns of anti-oxidants, biomarkers of oxidative stress, and Hsp60 levels in sera from newborns and found significant associations between glutathione (GSH) levels and gestational age, delivery modality, and lipid hydroperoxydes (LOOH) level. LOOH levels and spontaneous (vaginal) delivery were independently associated with increased GSH levels when these were above the median. Hsp60 and LOOH levels were positively correlated whereas Hsp60 and GSH levels were inversely correlated in spontaneously delivered newborns; in contrast, Hsp60 and GSH levels were positively correlated in newborns delivered by cesarea. Our results point to new directions in the search for definite patterns of GSH, LOOH, and Hsp60 in the newborn's serum that might have functional and diagnostic significance and that could help in the monitoring of newborn health during and after delivery. In addition, the data provide a starting basis for investigating the precise roles and interplay of GSH and Hsp60 in the maintenance of an optimal redox balance at birth to cope with the stress inherent to delivery, and also for investigating the predictive value of any given pattern of GSH, LOOH, and Hsp60 at birth with regard to health status and risk of disease in adult life.
Department of Sciences for Health Promotion and Mother and Child Care, Pediatric Unit, University of Palermo, Palermo, Italy.
Mario
M
Giuffrè
Biomedical Department of Internal Medicine and Medical Specialties, University of Palermo, Italy; Euro-Mediterranean Institute of Science and Technology, Palermo, Italy.
Manfredi
M
Rizzo
Department of Sciences for Health Promotion and Mother and Child Care, Pediatric Unit, University of Palermo, Palermo, Italy.
Giusy
G
Scaturro
Euro-Mediterranean Institute of Science and Technology, Palermo, Italy; Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Italy.
Alessandro
A
Pitruzzella
Euro-Mediterranean Institute of Science and Technology, Palermo, Italy; Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Italy.
Antonella
A
Marino Gammazza
Euro-Mediterranean Institute of Science and Technology, Palermo, Italy; Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Italy.
Francesco
F
Cappello
Department of Sciences for Health Promotion and Mother and Child Care, Pediatric Unit, University of Palermo, Palermo, Italy.
Giovanni
G
Corsello
Euro-Mediterranean Institute of Science and Technology, Palermo, Italy; Department of Biomedical and Biotechnological Sciences, University of Catania, Italy. Electronic address: livolti@unict.it.
Giovanni
G
Li Volti
D000328:Adult; D015415:Biomarkers / Q000097:blood; D018834:Chaperonin 60 / Q000097:blood*; D005260:Female; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D024101:Mitochondrial Proteins / Q000097:blood*; D018384:Oxidative Stress
D015415:Biomarkers; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D008054:Lipid Peroxides; D024101:Mitochondrial Proteins; D005978:Glutathione
Anti-stress molecules; Glutathione; Hsp60; Lipid hydroperoxydes; Neonatal stress; Oxidative-stress markers