Influence of 4 weeks of bovine colostrum supplementation on neutrophil and mucosal immune responses to prolonged cycling.
Bovine colostrum (COL) has been advocated as a nutritional countermeasure to exercise-induced immune dysfunction. The aims of this study were to identify the effects of 4 weeks of COL supplementation on neutrophil responses and mucosal immunity following prolonged exercise. In a randomized double-blind, parallel group design, participants [age 28 ± 8 years; body mass 79 ± 7 kg; height 182 ± 6 cm; maximal oxygen uptake (V̇O2max) 55 ± 9 mL/kg/min] were assigned to 20 g per day of COL (n = 10) or an isoenergetic/isomacronutrient placebo (PLA; n = 10) for 4 weeks. Venous blood and unstimulated saliva samples were obtained before and after 2.5 h of cycling at 15% Δ (∼55-60% V̇O2max). A significantly greater formyl-methionyl-leucyl phenylalanine-stimulated oxidative burst was observed in the COL group compared with PLA group (P < 0.05) and a trend toward a time × group interaction (P = 0.06). However, there was no effect of COL on leukocyte trafficking, phorbol-12-myristate-13-acetate-stimulated oxidative burst, bacterial-stimulated neutrophil degranulation, salivary secretory IgA, lactoferrin or lysozyme (P > 0.05). These findings provide further evidence of the beneficial effects of COL on receptor-mediated stimulation of neutrophil oxidative burst in a model of exercise-induced immune dysfunction.
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.|Clinical Research Centre, Prince Phillip Hospital, Llanelli, UK.
A W
AW
Jones
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
R
R
Thatcher
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
D S
DS
March
Endurance Research Group, School of Sport and Exercise Sciences, University of Kent at Medway, Chatham, UK.
G
G
Davison
D000328:Adult; D000818:Animals; D002417:Cattle; D015550:Cell Degranulation; D003126:Colostrum / Q000276:immunology*; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007958:Leukocyte Count; D009061:Mouth Mucosa / Q000276:immunology* / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology*; D061251:Primary Cell Culture; D016897:Respiratory Burst / Q000187:drug effects; D012463:Saliva / Q000276:immunology / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D055815:Young Adult
D007070:Immunoglobulin A; C016658:phorbolol myristate acetate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate
Immune function; antimicrobial peptides; exercise; innate immune system; leukocyte