Differential microfluidic sensor on printed circuit board for biological cells analysis.
Coulter principal based resistive pulse sensor has been demonstrated as an important platform in biological cell detection and enumeration since several decades ago. Recently, the miniaturized micro-Coulter counter has attracted much attention due to its advantages in point of care diagnostics for on chip detection and enumeration of rare cells, such as circulating tumor cells. In this paper, we present a microfluidic cytometer with differential amplifier based on Coulter principle on a SU-8 coated printed circuit board substrate. The electrical current changes induced by the blockage of the microparticles in the sensing aperture are calibrated by polystyrene particles of standard size. Finally, HeLa cells are used to evaluate the performance of the proposed device for enumeration of biological samples. The proposed cytometer is built upon the cheap and widely available printed circuit board substrate and shows its great potential as personalized healthcare monitor.
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, P. R. China.
Dongyuan
D
Shi
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, P. R. China.|Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore, Singapore.
Jinhong
J
Guo
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, P. R. China.
Liang
L
Chen
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, P. R. China.
Chuncheng
C
Xia
China Aerodynamics Research & Development Center; Mianyang, Sichuan, P. R. China.
Zhefeng
Z
Yu
Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore, Singapore.
Ye
Y
Ai
Institute for Clean Energy and Advanced Materials, Southwest University, Beibei, Chongqing, P. R. China.
Chang Ming
CM
Li
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
Yuejun
Y
Kang
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, P. R. China.
Zhiming
Z
Wang
D004581:Electronics / Q000295:instrumentation*; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009360:Neoplastic Cells, Circulating / Q000737:chemistry*; D057285:Precision Medicine / Q000295:instrumentation*
Circulating tumor cell; Coulter counter; PCB; Personalized healthcare monitor