Nuclear membrane R-type calcium channels mediate cytosolic ET-1-induced increase of nuclear calcium in human vascular smooth muscle cells.
The objective of this work was to verify whether, as in the case of the plasma membrane of human vascular smooth muscle cells (hVSMCs), cytosolic ET-1-induced increase of nuclear calcium is mediated via the activation of calcium influx through the steady-state R-type calcium channel. Pharmacological tools to identify the R-type calcium channels, as well as real 3-D confocal microscopy imaging techniques coupled to calcium fluorescent probes, were used to study the effect of cytosolic ET-1 on nuclear calcium in isolated nuclei of human hepatocytes and plasma membrane perforated hVSMCs. Our results showed that pre-treatment with pertussis toxin (PTX) or cholera toxin (CTX) prevented cytosolic ET-1 (10(-9) mol/L) from inducing a sustained increase in nuclear calcium. Furthermore, the L-type calcium channel blocker nifedipine did not prevent cytosolic ET-1 from inducing an increase in nuclear calcium, as opposed to the dual L- and R-type calcium channel blocker isradipine (PN200-110) (in the presence of nifedipine). In conclusion, the preventative effect with PTX and CTX, and the absence of an effect with nifedipine, as well as the blockade by isradipine on cytosolic ET-1-induced increase in nuclear calcium, suggest that this nuclear calcium influx in hVSMCs is due to activation of the steady-state R-type calcium channel. The sarcolemmal and nuclear membrane R-type calcium channels in hVSMCs are involved in ET-1 modulation of vascular tone in physiology and pathology.
Department of Anatomy and Cell Biology, Faculty of Medicine - University of Sherbrooke, 3001, 12th Avenue North, Sherbrooke, QC J1H 5N4, Canada.
Ghassan
G
Bkaily
Levon
L
Avedanian
Johny
J
Al-Khoury
Marc
M
Chamoun
Rana
R
Semaan
Cynthia
C
Jubinville-Leblanc
Pedro
P
D'Orléans-Juste
Danielle
D
Jacques
D001011:Aorta; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020908:Calcium Channels, R-Type / Q000737:chemistry / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D027682:Cation Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D008561:Membrane Fusion / Q000187:drug effects; D049990:Membrane Transport Modulators / Q000494:pharmacology; D018613:Microscopy, Confocal; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism*; D037342:Pertussis Toxin / Q000494:pharmacology; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism
C423009:CACNA1E protein, human; D002121:Calcium Channel Blockers; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D019332:Endothelin-1; D049990:Membrane Transport Modulators; D002772:Cholera Toxin; D037342:Pertussis Toxin
CTX; PTX; RCPG de la membrane nucléaire; calcium transport; isradipine; nifedipine; nifédipine; noyau; nuclear membrane GPCR; nucleus; transport de calcium