A novel chromatographic media: histidine-containing composite cryogels for HIgG separation from human serum.
Histidine-containing microspheres (HCM) with 2 μm in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.
Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey.
Gözde
G
Baydemir
Mehmet
M
Odabaşı
D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods*; D059985:Cryogels / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry; D008863:Microspheres; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000178:Acrylamides; D002021:Buffers; D059985:Cryogels; D007074:Immunoglobulin G; D008689:Methacrylates; D014867:Water; D011102:Polyhydroxyethyl Methacrylate; D006639:Histidine; C005044:hydroxyethyl methacrylate; C021221:N,N'-methylenebisacrylamide