Gene silencing by DNA interference in fern gametophytes.
RNA interference is commonly used for posttranscriptional silencing of target gene transcripts. In fern gametophytes, however, sequence-specific gene silencing is possible by introducing double-stranded DNA fragments into gametophyte cells by particle bombardment. Silencing could be transmitted all over the gametophyte through live cells. Further, inheritance of the gene silencing to the progeny is depending on the gene used. Here we describe how to introduce the DNA fragments into the gametophyte cells and how to screen the DNA-transferred cells.
Biology Department, Faculty of Science, Kyushu University, 6-10-1 Hakozaki, Higashi Ward, Fukuoka, 812-8581, Japan, wadascb@kyushu-u.org.
Masamitsu
M
Wada
Hidenori
H
Tsuboi
D004247:DNA / Q000235:genetics*; D029624:Ferns / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques / Q000379:methods*; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors / Q000235:genetics; D055993:Germ Cells, Plant / Q000254:growth & development*
D004247:DNA