Immunomodulatory Activities on RAW 264.7 Macrophages of a Polysaccharide from Veiled Lady Mushroom, Dictyophora indusiata (Higher Basidiomycetes).
A water-soluble polysaccharide named DI was extracted from the fruiting bodies of gastroid mushroom Dictyophora indusiata with boiling water. The chemical and physical characteristics of DI were investigated by a combination of chemical and instrumental analysis methods. The immunomodulatory activities on RAW 264.7 macrophage of DI in vitro were also studied. The results showed that DI is a β-(1→3)-glucan with side branches of β-(1→6)-glucosyl units, and it has triple-helical structure. DI has no toxic effect on cells, but can promote macrophage multiplication. DI significantly affects the immune function by promoting the production of nitric oxide and cytokines, such as tumor necrosis factor-α, interleukin-1, -6, and -12, showing an obvious dose-effect relationship. This work extends the application scope of the polysaccharide from D. indusiata in the biomedical field.
School of Pharmaceutics Science, Jiangnan University, Wuxi 214122, People's Republic of China.
Haitian
H
Fu
Wuxi Medical School, Jiangnan University, Wuxi 214122, People's Republic of China.
Chao
C
Deng
Wuxi Medical School, Jiangnan University, Wuxi 214122, People's Republic of China.
Liping
L
Teng
School of Pharmaceutics Science, Jiangnan University, Wuxi 214122, People's Republic of China.
Li
L
Yu
Wuxi No. 2 People's Hospital, Wuxi 214002, People's Republic of China.
Tong
T
Su
Wuxi No. 2 People's Hospital, Wuxi 214002, People's Republic of China.
Xin
X
Xu
School of Pharmaceutics Science, Jiangnan University, Wuxi 214122, People's Republic of China.
Jinghua
J
Chen
Wuxi No. 2 People's Hospital, Wuxi 214002, People's Republic of China.
Chengjian
C
Yang
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D048690:Fruiting Bodies, Fungal; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D007109:Immunity / Q000187:drug effects*; D007155:Immunologic Factors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D000067996:RAW 264.7 Cells; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
D001688:Biological Products; D016207:Cytokines; D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D047071:beta-Glucans; D009569:Nitric Oxide