Identification and characterization of novel serum microRNAs in unstable angina pectoris and subclinical atherosclerotic patients.
BACKGROUND
MicroRNAs (miRNAs) are involved in cardiac developmental and pathological processes, and serum profile is useful for identifying novel miRNAs.


RESULTS
Serum samples were collected from unstable angina pectoris (UAP) and subclinical atherosclerotic (AS) patients. Solexa sequencing was used to predict novel miRNAs in 15 control individuals, 15 AS patients and 15 UAP patients. After bioinformatics analysis and filtering out in the newest version of miRbase (version 20.0), three novel miRNAs were validated in 80 control individuals, 80 AS patients and 80 UAP patients by quantitative reverse transcriptase polymerase chain reaction. Two of the three novel microRNAs (N1 and N3) were expressed at the highest levels in the AS group. N1 had an area under curve (AUC) of 0.811 (95% confidence interval 0.743-0.880) for AS. N3 showed a moderate separation with an area under curve (AUC) of 0.748 (95% confidence interval 0.664-0.833) for AS. Combined the two novel microRNAs can significantly distinguish AS from control.


CONCLUSIONS
Three novel miRNAs were identified by Solexa sequencing and two of them may be new potential predictors for arthrosclerosis.
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China; Department of Clinical Laboratory, Affiliated Hospital of Chengde Medical University, No. 36 Nanyingzi Road, Chengde 067000, China.
Qiyu
Q
Sun
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Xingwang
X
Jia
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Jing
J
Gao
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Pengjun
P
Zhang
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Wenjun
W
Mou
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Caie
C
Yang
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Hongli
H
Tong
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
Xinyu
X
Wen
Department of Clinical Biochemistry, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China. Electronic address: 13683048264@163.com.
Yaping
Y
Tian
D000789:Angina, Unstable / Q000097:blood*; D050197:Atherosclerosis / Q000097:blood*; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D008969:Molecular Sequence Data; D012372:ROC Curve
D015415:Biomarkers; D035683:MicroRNAs
Atherosclerosis; Solexa; Unstable angina pectoris; microRNA