Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.
Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ≤ 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening.
Laboratorio de Genética y Biología Molecular de Plantas, Centro de Investigación en Alimentación y Desarrollo Hermosillo, Sonora, Mexico.
Mitzuko
M
Dautt-Castro
Instituto Nacional de Medicina Genómica, Unidad de Genómica de Poblaciones, Aplicada a la Salud, Facultad de Qumica UNAM, Delegación Tlalpan, Mexico DF.
Adrian
A
Ochoa-Leyva
Laboratorio de Genética y Biología Molecular de Plantas, Centro de Investigación en Alimentación y Desarrollo Hermosillo, Sonora, Mexico.
Carmen A
CA
Contreras-Vergara
Laboratorio de Genética y Biología Molecular de Plantas, Centro de Investigación en Alimentación y Desarrollo Hermosillo, Sonora, Mexico.
Magda A
MA
Pacheco-Sanchez
Laboratorio de Genómica Funcional y Comparativa, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica San Luis Potosí (SLP), Mexico.
Sergio
S
Casas-Flores
Unidad Universitaria de Secuenciación Masiva de DNA, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Morelos, Mexico.
Alejandro
A
Sanchez-Flores
United States Department of Agriculture - Agricultural Research Service, Subtropical Horticulture Research Station Miami, FL, USA.
David N
DN
Kuhn
Laboratorio de Genética y Biología Molecular de Plantas, Centro de Investigación en Alimentación y Desarrollo Hermosillo, Sonora, Mexico.
Maria A
MA
Islas-Osuna
Mangifera indica L.; cell wall hydrolytic enzymes; ethylene; fruit quality; fruit ripening; mesocarp; transcriptome