X-box-binding protein 1-modified neural stem cells for treatment of Parkinson's disease.
X-box-binding protein 1-transfected neural stem cells were transplanted into the right lateral ventricles of rats with rotenone-induced Parkinson's disease. The survival capacities and differentiation rates of cells expressing the dopaminergic marker tyrosine hydroxylase were higher in X-box-binding protein 1-transfected neural stem cells compared to non-transfected cells. Moreover, dopamine and 3,4-dihydroxyphenylacetic acid levels in the substantia nigra were significantly increased, α-synuclein expression was decreased, and neurological behaviors were significantly ameliorated in rats following transplantation of X-box-binding protein 1-transfected neural stem cells. These results indicate that transplantation of X-box-binding protein 1-transfected neural stem cells can promote stem cell survival and differentiation into dopaminergic neurons, increase dopamine and 3,4-dihydroxyphenylacetic acid levels, reduce α-synuclein aggregation in the substantia nigra, and improve the symptoms of Parkinson's disease in rats.
Department of Gynecology and Obstetrics, Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
Lihui
L
Si
Department of Gynecology and Obstetrics, Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
Tianmin
T
Xu
Department of Neurology, Norman Bethune First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
Fengzhang
F
Wang
Department of Neurology, Norman Bethune First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
Qun
Q
Liu
Department of Gynecology and Obstetrics, Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
Manhua
M
Cui
Parkinson's disease; X-box-binding protein 1; neural stem cells; α-synuclein; dopamine