In vivo study on the neurotransmitters and their metabolites change in depressive disorder rat plasma by ultra high performance liquid chromatography coupled to tandem mass spectrometry.
A sensitive and versatile, ultra-high performance, liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method coupled to pre-column derivatization for the simultaneous determination of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), dopamine (DA), norepinephrine (NE), homovanillic acid (HVA), γ-aminobutyric acid (GABA) and glutamic acid (Glu) was developed and validated in rat plasma. The analytes were dansylated under strong alkaline conditions after protein precipitation extraction, which were analyzed on a BEH C18 column using a gradient elution. The lower limit of quantification (LLOQ) values for 5-HT, 5-HIAA, DA, NE, HVA, GABA and Glu were 1.00, 1.00, 0.991, 0.992, 1.02, 1000, and 5030 pmol/mL, respectively. Good linearity was obtained (r > 0.99) and the intra- and inter-day precisions of the method (relative standard deviation, RSD%) were lower than 12%. The method was novel, sensitive and specific which can provide an alternative method for the quantification of neurotransmitters and their metabolites in plasma samples.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Longshan
L
Zhao
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Shuning
S
Zheng
Department of Pharmacology, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, China.
Guangyue
G
Su
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Xiumei
X
Lu
Department of Pharmacology, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, China.
Jingyu
J
Yang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: bearry200@126.com.
Zhili
Z
Xiong
Department of Pharmacology, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, China. Electronic address: chunfuw@gmail.com.
Chunfu
C
Wu
D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003866:Depressive Disorder / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D016014:Linear Models; D008297:Male; D018377:Neurotransmitter Agents / Q000097:blood* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers; D018377:Neurotransmitter Agents
Biomarker; Fluoxetine; Monoamine neurotransmitters; Tandem mass spectrometry