Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea.
Chlamydia pecorum (designated 22-58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C. pecorum positive reactions in the jejunal villi. C. pecorum, designated 24-100, was isolated from the feces of a calf with diarrhea in another farm in Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers against C. pecorum was confirmed in paired sera. Nucleotide sequence identities of omp1 genes of the 2 isolates were 100%. The isolates were genetically and antigenically more closely related to C. pecorum Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from sheep with polyarthritis. These results indicate that C. pecorum strains similar to 22-58 and 24-100 might be endemic in Yamaguchi Prefecture and cause enteric disease in cattle.
Yamaguchi Chubu Livestock Hygiene Service Center, 671-5 Kagawa, Yamaguchi 754-0897, Japan.
Akifumi
A
Ohtani
Masahito
M
Kubo
Hiroshi
H
Shimoda
Kenji
K
Ohya
Tadashi
T
Iribe
Daiki
D
Ohishi
Daiji
D
Endoh
Tsutomu
T
Omatsu
Tetsuya
T
Mizutani
Hideto
H
Fukushi
Ken
K
Maeda
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002689:Chlamydia / Q000235:genetics* / Q000276:immunology; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D007422:Intestines / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary
D000942:Antigens, Bacterial