Expression of fructose 1,6-bisphosphatase and phosphofructokinase is induced in hepatopancreas of the white shrimp Litopenaeus vannamei by hypoxia.
Marine organisms are exposed to hypoxia in natural ecosystems and during farming. In these circumstances marine shrimp survive and synthesize ATP by anaerobic metabolism. Phosphofructokinase (PFK) and fructose 1,6-bisphosphatase (FBP) are key enzymes in carbohydrate metabolism. Here we report the cDNA of FBP from the shrimp Litopenaeus vannamei hepatopancreas and expression of PFK and FBP under normoxia and hypoxia. Hypoxia induces PFK and FBP expression in hepatopancreas but not in gills and muscle. Induction in hepatopancreas of the glycolytic and gluconeogenic key enzymes, PFK and FBP, suggests that PFK could be a key factor for increasing anaerobic rate, while FBP is probably involved in the activation of gluconeogenesis or the pentose-phosphates pathway during hypoxia in the highly active metabolism of hepatopancreas.
Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
Keni
K
Cota-Ruiz
Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
Alma B
AB
Peregrino-Uriarte
Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
Monserrath
M
Felix-Portillo
Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
José A
JA
Martínez-Quintana
Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico. Electronic address: gyepiz@ciad.mx.
Gloria
G
Yepiz-Plascencia
D000595:Amino Acid Sequence; D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D043143:Hepatopancreas / Q000201:enzymology / Q000503:physiopathology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics*; D025221:Phosphofructokinases / Q000235:genetics*; D016415:Sequence Alignment
D025221:Phosphofructokinases; D006597:Fructose-Bisphosphatase
Expression; Fructose 1,6-bisphosphatase (FBP); Gluconeogenesis; Glycolysis; Hypoxia; Phosphofructokinase (PFK)