Reactive oxygen species mediate visceral pain-related amygdala plasticity and behaviors.
Accumulating evidence suggests an important contribution of reactive oxygen species (ROS) to pain and neuropsychiatric disorders, but their role in pain-related plasticity in the brain is largely unknown. Neuroplasticity in the central nucleus of the amygdala (CeA) correlates positively with pain behaviors in different models. Little is known, however, about mechanisms of visceral pain-related amygdala changes. The electrophysiological and behavioral studies reported here addressed the role of ROS in the CeA in a visceral pain model induced by intracolonic zymosan. Vocalizations to colorectal distension and anxiety-like behavior increased after intracolonic zymosan and were inhibited by intra-CeA application of a ROS scavenger (tempol, a superoxide dismutase mimetic). Tempol also induced a place preference in zymosan-treated rats but not in controls. Single-unit recordings of CeA neurons in anesthetized rats showed increases of background activity and responses to visceral stimuli after intracolonic zymosan. Intra-CeA application of tempol inhibited the increased activity but had no effect under normal conditions. Whole-cell patch-clamp recordings of CeA neurons in brain slices from zymosan-treated rats showed that tempol decreased neuronal excitability and excitatory synaptic transmission of presumed nociceptive inputs from the brainstem (parabrachial area) through a combination of presynaptic and postsynaptic actions. Tempol had no effect in brain slices from sham controls. The results suggest that ROS contribute to visceral pain-related hyperactivity of amygdala neurons and amygdala-dependent behaviors through a mechanism that involves increased excitatory transmission and excitability of CeA neurons.
Department of Pharmacology and Neuroscience, School of Medicine, Texas Tech University Health Sciences Center (TTUHSC), Lubbock, TX Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, The Fourth Military Medical University, Xi'an, China.
Guangchen
G
Ji
Zhen
Z
Li
Volker
V
Neugebauer
D000679:Amygdala / Q000166:cytology / Q000187:drug effects / Q000503:physiopathology*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001007:Anxiety / Q000188:drug therapy / Q000523:psychology; D003497:Cyclic N-Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004594:Electrophysiology / Q000379:methods; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013113:Spin Labels; D009435:Synaptic Transmission / Q000187:drug effects; D059265:Visceral Pain / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology* / Q000523:psychology*; D014828:Vocalization, Animal / Q000187:drug effects; D015054:Zymosan / Q000633:toxicity
D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D015054:Zymosan; C001803:tempol