Development and application of an analytical method for curdione quantification in pregnant Sprague-Dawley rats by LC-MS/MS.
The vaginal administration route suffers from relatively low absorption efficiency, which may hinder the identification of the toxicokinetics of curdione in pregnant women. A sensitive analytical method for determining the plasma concentration of curdione was developed and applied in the determination of curdione in pregnant Sprague-Dawley rats as a simulated model. Glimepiride was used as an internal standard and chromatographic separation was achieved on a Capcell Pak C18 MGIII column. A gradient elution profile with 0.5% formic acid (A)-0.5% formic acid-acetonitrile (B) was selected as mobile phase. The selected reaction monitoring mode was used for quantification based on the target fragment ions m/z 237.2 to m/z 135.1 for curdione and m/z 491.3 to m/z 352.1 for the glimepiride. The standard curve was linear over the range of 0.5-500 ng/mL for curdione in rat plasma and yielded a consistent peak pattern, even at the lower limit of quantitation of 0.5 ng/mL. The retention times of curdione and IS were 6.55 and 6.59 min, respectively. The mean recovery of curdione in rat plasma was 95.5-101.1%. The intra-day and inter-day precisions were between 2.35 and 9.08%. This LC-MS/MS method provides a simple and sensitive means for determining the plasma concentration.
Pharmacy School of Fudan University, 2140 Xie Tu Road, Shanghai, People's Republic of China.|Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Xiang
http://orcid.org/0000-0001-8249-5243
X
Meng
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Ting
T
Zhang
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Ying
Y
Li
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Qi
Q
Pan
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Juan
J
Jiang
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Yongwei
Y
Luo
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Liming
L
Chong
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Yang
Y
Yang
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Sichong
S
Xu
Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Li
L
Zhou
Pharmacy School of Fudan University, 2140 Xie Tu Road, Shanghai, People's Republic of China.|Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai, 200032, People's Republic of China.
Zuyue
Z
Sun
D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D045788:Sesquiterpenes, Germacrane / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D013488:Suppositories; D053719:Tandem Mass Spectrometry / Q000379:methods*
D045788:Sesquiterpenes, Germacrane; D013488:Suppositories; C504126:curdione
LC-MS/MS; curdione; plasma; toxicokinetics