Structure and genetics of the O-antigen of Enterobacter cloacae G3054 containing di-N-acetylpseudaminic acid.
Mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3054 resulted in the cleavage of the O-polysaccharide at the linkage of residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac) in the main chain. The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, and the following structure of the branched pentasaccharide O-unit of the O-polysaccharide was established: [structure: see text] The O-antigen gene cluster of E. cloacae G3054 between conserved genes galF and gnd was sequenced. Most genes necessary for the O-antigen synthesis were found in the cluster and their functions were tentatively assigned by comparison with sequences in the available databases.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
Andrei V
AV
Perepelov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Min
M
Wang
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Andrei V
AV
Filatov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Xi
X
Guo
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Alexander S
AS
Shashkov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; Tianjin Biochip Corporation, TEDA, Tianjin 300457, China.
Lei
L
Wang
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Yuriy A
YA
Knirel
D002240:Carbohydrate Sequence; D016972:Enterobacter cloacae / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D066244:Proton Magnetic Resonance Spectroscopy; D012794:Sialic Acids / Q000737:chemistry*
C502287:5,7-diacetamido-3,5,7,9-tetradeoxynon-2-ulosonic acid; D008070:Lipopolysaccharides; D019081:O Antigens; D012794:Sialic Acids
Bacterial polysaccharide structure; Enterobacter cloacae; O-antigen; O-antigen gene cluster; Pseudaminic acid