Tri-allelic pattern of short tandem repeats identifies the murderer among identical twins and suggests an embryonic mutational origin.
Monozygotic twins can be co-identified by genotyping of short tandem repeats (STRs); however, for distinguishing them, STR genotyping is ineffective, especially in the case of murder. Here, a rarely occurring tri-allelic pattern in the vWA locus (16, 18, 19) was identified only in the DNA of one identical twin, which could help to exonerate the innocent twin in a murder charge. This mutation was defined as primary through genotyping of the family and could be detected in blood, buccal and semen samples from the individual; however, two alternative allele-balanced di-allelic patterns (16, 18 or 16, 19) were detected in hair root sheath cells. Such a kind of segregation indicates a one-step mutation occurs in cell mitosis, which is after embryonic zygote formation and during the early development of the individual after the division of the blastocyte. Sequencing revealed the insertion between the allele 18 and 19 is a repeat unit of TAGA/TCTA (plus/minus strand), which belongs to "AGAT/ATCT"-based core repeats identified from all tri-allelic pattern reports recorded in the STR base and a detailed model was proposed for STR repeat length variation caused by false priming during DNA synthesis. Our model illustrates the possible origination of allele-balanced and unbalanced tri-allelic pattern, clarifies that the genotypes of parent-child mismatches, aberrant di-allelic patterns, and type 1 or 2 tri-allelic patterns should be considered as independent, but interconnected forms of STR mutation.
Department of Biochemistry and Molecular Biology, Xi'an, Shaanxi, China; Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: lfwang@fmmu.edu.cn.
Li-Feng
LF
Wang
Department of Biochemistry and Molecular Biology, Xi'an, Shaanxi, China; Center for DNA Typing, Xi'an, Shaanxi, China; Fourth Military Medical University, Xi'an, Shaanxi, China.
Ying
Y
Yang
Department of Biochemistry and Molecular Biology, Xi'an, Shaanxi, China; Center for DNA Typing, Xi'an, Shaanxi, China; Fourth Military Medical University, Xi'an, Shaanxi, China.
Xiao-Nan
XN
Zhang
Public Security Bureau, Qi'shan, Shaanxi, China.
Xiao-Liang
XL
Quan
Department of Biochemistry and Molecular Biology, Xi'an, Shaanxi, China; Center for DNA Typing, Xi'an, Shaanxi, China; Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: wuym@fmmu.edu.cn.
Yuan-Ming
YM
Wu
D000483:Alleles; D006708:Homicide; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation; D014430:Twins, Monozygotic
Identical twins; STR mutation; Tri-allelic pattern