Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide.
Aquaporins are water channel proteins that mediate the fine-tuning of cell membrane water permeability during development or in response to environmental stresses. The present work focuses on the oxidative stress-induced redistribution of plasma membrane intrinsic protein (PIP) aquaporins from the plasma membrane (PM) to intracellular membranes. This process was investigated in the Arabidopsis root. Sucrose density gradient centrifugation showed that exposure of roots to 0.5 mM H2O2 induces significant depletion in PM fractions of several abundant PIP homologs after 15 min. Analyses by single-particle tracking and fluorescence correlative spectroscopy showed that, in the PM of epidermal cells, H2O2 treatment induces an increase in lateral motion and a reduction in the density of a fluorescently tagged form of the prototypal AtPIP2;1 isoform, respectively. Co-expression analyses of AtPIP2;1 with endomembrane markers revealed that H2O2 triggers AtPIP2;1 accumulation in the late endosomal compartments. Life-time analyses established that the high stability of PIPs was maintained under oxidative stress conditions, suggesting that H2O2 triggers a mechanism for intracellular sequestration of PM aquaporins without further degradation. In addition to information on cellular regulation of aquaporins, this study provides novel and complementary insights into the dynamic remodeling of plant internal membranes during oxidative stress responses.
Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 2, Place Viala, F-34060 Montpellier Cedex 2, France.
Michael M
MM
Wudick
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Xiaojuan
X
Li
Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 2, Place Viala, F-34060 Montpellier Cedex 2, France.
Valeria
V
Valentini
Department of Plant Molecular Biology, Université de Lausanne, 1015 Lausanne, Switzerland.
Niko
N
Geldner
Plant Molecular and Cellular Biology Laboratory, The Salk Institute, La Jolla, CA 92037, USA.
Joanne
J
Chory
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Jinxing
J
Lin
Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 2, Place Viala, F-34060 Montpellier Cedex 2, France. Electronic address: maurel@supagro.inra.fr.
Christophe
C
Maurel
Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 2, Place Viala, F-34060 Montpellier Cedex 2, France. Electronic address: luu@supagro.inra.fr.
Doan-Trung
DT
Luu
D020346:Aquaporins / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004058:Diffusion / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects
D020346:Aquaporins; D029681:Arabidopsis Proteins; C491680:plasma membrane intrinsic protein 2 Arabidopsis; D006861:Hydrogen Peroxide
Arabidopsis; aquaporin; reactive oxygen species; root; stress