Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro.
Pancreatic stem cells were isolated and cultured from aborted human fetal pancreases of gestational age 14-20 weeks. They were seeded at a density of 1 × 10(4) in serum-free media for differentiation into neuron-like cells, expressing β-tubulin III and glial fibrillary acidic protein. These neuron-like cells displayed a synapse-like morphology and appeared to form a neuronal network. Pancreatic stem cells were also seeded at a density of 1 × 10(5) for differentiation into islet-like cells, expressing insulin and glucagon, with an islet-like morphology. These cells had glucose-stimulated secretion of human insulin and C-peptide. Results suggest that pancreatic stem cells can be differentiated into neuron-like and islet-like cells.
Department of Endocrinology, Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Xiufeng
X
Hua
Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Yanwei
Y
Wang
Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Peiwen
P
Lian
Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Shouxin
S
Zhang
Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Jianyuan
J
Li
Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Haiyan
H
Wang
Department of Endocrinology, Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Shulin
S
Chen
Department of Endocrinology, Yuhuangding Hospital, Yantai 264000, Shandong Province, China.
Wei
W
Gao
differentiation; fetal pancreas; islet-like cells; neural regeneration; neuron-like cells; pancreatic stem cells