Nutrition-dependent changes of mouse adipose tissue compositions monitored by NMR, MS, and chromatographic methods.
Many diseases nowadays are assumed to be genetically determined. Therefore, many knockout mouse models have been established and are widely used. Unfortunately, nutrition (in particular the fat content of food) is often neglected in studies on these disease models. In this study the effects of nutrition on the lipid (triacylglycerol, TAG) compositions of different mouse adipose tissues were investigated. Mice were subjected to different diets [high fat (HF) vs. standard diet (SD)] and different adipose tissue samples (brown, visceral, and subcutaneous fat) were isolated after 12 weeks. Subsequent to lipid extraction, the organic extracts were analyzed by mass spectrometry (MALDI and ESI), high-resolution (1)H and (31)P NMR spectroscopy, high-performance thin-layer chromatography (HPTLC), and gas chromatography (GC). In adipose tissue of mice fed with HF diet, (a) decreased double bond contents and (b) decreased fatty acyl chain lengths of tissue TAGs were observed; this trend could be concomitantly monitored by all methods used. However, the adipose tissue still contained significant amounts of slightly unsaturated fatty acyl residues (18:1). Thus, a certain double bond content seems necessary to maintain the properties of adipose tissues.
Medical Faculty, Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany.
Yulia
Y
Popkova
Andrej
A
Meusel
Jana
J
Breitfeld
Dorit
D
Schleinitz
Johannes
J
Hirrlinger
Dirk
D
Dannenberger
Peter
P
Kovacs
Jürgen
J
Schiller
D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000032:analysis / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000032:analysis* / Q000378:metabolism
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D014280:Triglycerides