P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts.
The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China.
Xiao-Ya
XY
Liu
Jian
J
Yang
Li
L
Xie
Jing
J
Li
Xi-Jiao
XJ
Song
Jian-Ping
JP
Chen
Heng-Mu
HM
Zhang
D015153:Blotting, Western; D002736:Chloroplasts / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D012275:Oryza; D021381:Protein Transport; D012087:Reoviridae / Q000502:physiology*; D014026:Nicotiana; D017361:Viral Nonstructural Proteins / Q000032:analysis*; D014771:Virion / Q000737:chemistry*
D017361:Viral Nonstructural Proteins