Isolation of human mesenchymal stem cells and their cultivation on the porous bone matrix.
Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbone (NKB) as a scaffold. Thus, the application of MSCs in repair and tissue regeneration processes depends principally on the efficient implementation of the techniques for placing these cells in a host tissue. For this reason, the design of biomaterials and cellular scaffolds has gained importance in recent years because the topographical characteristics of the selected scaffold must ensure adhesion, proliferation and differentiation into the desired cell lineage in the microenvironment of the injured tissue. This option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) employs bovine bone matrix as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of the bovine bone matrix Nukbone (NKB), i.e., spreading on the surface, macroporous covering and colonizing the depth of the biomaterial, after the cell isolation process. We present the procedure for isolating and culturing MSCs on a bovine matrix.
Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM).
Nayeli
N
Rodríguez-Fuentes
Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM).
Olivia
O
Reynoso-Ducoing
Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM).
Ana
A
Rodríguez-Hernández
Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM).
Javier R
JR
Ambrosio-Hernández
Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM).
Maria C
MC
Piña-Barba
Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM).
Armando
A
Zepeda-Rodríguez
Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM).
Marco A
MA
Cerbón-Cervantes
Depto. Genetica y Biología Molecular, Cinvestav-IPN.
José
J
Tapia-Ramírez
Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud; luzalcantara@gmail.com.
Luz E
LE
Alcantara-Quintana
D000650:Amnion / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials; D001857:Bone Matrix; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D054457:Tissue Scaffolds
D001672:Biocompatible Materials