Simultaneous generation of many RNA-seq libraries in a single reaction.
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Alexander A
AA
Shishkin
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Georgia
G
Giannoukos
Bioinformatics Core, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Alper
A
Kucukural
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Dawn
D
Ciulla
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Michele
M
Busby
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Christine
C
Surka
1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Jenny
J
Chen
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Roby P
RP
Bhattacharyya
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Robert F
RF
Rudy
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Milesh M
MM
Patel
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Nathaniel
N
Novod
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Deborah T
DT
Hung
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Andreas
A
Gnirke
1] Bioinformatics Core, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Manuel
M
Garber
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Mitchell
M
Guttman
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Jonathan
J
Livny
D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D020869:Gene Expression Profiling / Q000592:standards; D015723:Gene Library; D017423:Sequence Analysis, RNA / Q000191:economics / Q000379:methods* / Q000592:standards; D013997:Time Factors