Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury.
In the present study, human umbilical cord blood mesenchymal stem cells were injected into a rat model of traumatic brain injury via the tail vein. Results showed that 5-bromodeoxyuridine-labeled cells aggregated around the injury site, surviving up to 4 weeks post-transplantation. In addition, transplantation-related death did not occur, and neurological functions significantly improved. Histological detection revealed attenuated pathological injury in rat brain tissues following human umbilical cord blood mesenchymal stem cell transplantation. In addition, the number of apoptotic cells decreased. Immunohistochemistry and in situ hybridization showed increased expression of brain-derived neurotrophic factor, nerve growth factor, basic fibroblast growth factor, and vascular endothelial growth factor, along with increased microvessel density in surrounding areas of brain injury. Results demonstrated migration of transplanted human umbilical cord blood mesenchymal stem cells into the lesioned boundary zone of rats, as well as increased angiogenesis and expression of related neurotrophic factors in the lesioned boundary zone.
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Junjian
J
Zhao
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Naiyao
N
Chen
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Na
N
Shen
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China ; Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China.
Hui
H
Zhao
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Dali
D
Wang
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Jun
J
Shi
College of Life Science, Hebei United University, Tangshan 063000, Hebei Province, China.
Yang
Y
Wang
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Xiufeng
X
Cui
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Zhenyu
Z
Yan
Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China.
Hui
H
Xue
angiogenesis; basic fibroblast growth factor; brain-derived neurotrophic factor; human umbilical cord blood mesenchymal stem cells; nerve growth factor; traumatic brain injury; vascular endothelial growth factor