Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma.
OBJECTIVE
Hyperbaric oxygen therapy (HBOT) is used in the treatment of radiation-induced tissue injury but its effect on (residual) tumor tissue is indistinct and therefore investigated in this study.


METHODS
Orthotopic FaDu tumors were established in mice, and the response of the (irradiated) tumors to HBOT was monitored by bioluminescence imaging. Near infrared fluorescence imaging using AngioSense750 and Hypoxisense680 was applied to detect tumor vascular permeability and hypoxia.


RESULTS
HBOT treatment resulted in accelerated growth of non-irradiated tumors, but mouse survival was improved. Tumor vascular leakiness and hypoxia were enhanced after HBOT, whereas histological characteristics, epithelial-to-mesenchymal transition markers, and metastatic incidence were not influenced.


CONCLUSIONS
Squamous cell carcinoma responds to HBOT with respect to tumor growth, vascular permeability, and hypoxia, which may have implications for its use in cancer patients. The ability to longitudinally analyze tumor characteristics highlights the versatility and potential of optical imaging methods in oncological research.
Department of Oral and Maxillofacial Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.braks@erasmusmc.nl.
Joanna A M
JA
Braks
Department of Oral and Maxillofacial Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Linda
L
Spiegelberg
Department of Pathology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Senada
S
Koljenovic
Department of Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Yanto
Y
Ridwan
Department of Otorhinolaryngology and Head & Neck Surgery, Erasmus Medical Center, PO Box 1738, 3015 CE, Rotterdam, Netherlands.
Stijn
S
Keereweer
Department of Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.|Department of Radiation Oncology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Roland
R
Kanaar
Department of Oral and Maxillofacial Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Eppo B
EB
Wolvius
Department of Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.|Department of Radiation Oncology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.|Department of Vascular Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.
Jeroen
J
Essers
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology* / Q000628:therapy*; D006801:Humans; D006931:Hyperbaric Oxygenation; D051379:Mice; D008807:Mice, Inbred BALB C; D061848:Optical Imaging / Q000379:methods*; D010100:Oxygen / Q000627:therapeutic use
D010100:Oxygen
Animal model; Head and neck cancer; Hyperbaric oxygen therapy; Lymph node metastasis; Near infrared fluorescence; Optical imaging; Radiation; Squamous cell carcinoma