Role of Mucin 1 and Glycodelin A in recurrent implantation failure.
OBJECTIVE
To evaluate and compare the levels of Mucin 1 (MUC-1) and Glycodelin A (GdA) in precisely timed endometrial biopsies and blood samples taken from women with recurrent implantation failure, and women with proven fertility, in a control group.


METHODS
Molecular studies in human blood and tissue.


METHODS
University hospital.


METHODS
Women with recurrent implantation failure and women with proven fertility.


METHODS
Primary endometrial cells and blood samples during the implantation "window" (between day 7 and day 9 after the surge in luteinizing hormone).


METHODS
Expression of MUC-1 and GdA in the human endometrium and in blood during the implantation window were analyzed by enzyme-linked immunosorbent assay. Additionally, MUC-1 and GdA levels in tissue were analyzed by western blot during the same period.


RESULTS
Both blood and tissue measurements of MUC-1 and GdA were significantly lower in women with recurrent implantation failure than in fertile women during the implantation window. In addition, we found a highly significant correlation between blood vs. tissue measurements of both MUC-1 and GdA.


CONCLUSIONS
The present study reveals that blood and tissue levels of MUC-1 and GdA are much lower in women with RIF, compared with those in fertile women. Receptivity can be evaluated with noninvasive blood sampling, rather than more-invasive endometrium sampling, as the blood and tissue measurements of MUC-1 and GdA are correlated.
Department of Obstetrics and Gynecology, Istanbul University School of Medicine, Istanbul, Turkey. Electronic address: ercan.bastu@istanbul.edu.tr.
Ercan
E
Bastu
Health Research System Women's Hospital, Ankara, Turkey.
Mehmet F
MF
Mutlu
Department of Obstetrics and Gynecology, Istanbul University School of Medicine, Istanbul, Turkey.
Cenk
C
Yasa
Department of Obstetrics and Gynecology, Istanbul University School of Medicine, Istanbul, Turkey.
Ozlem
O
Dural
Department of Obstetrics and Gynecology, Istanbul University School of Medicine, Istanbul, Turkey.
Asli
A
Nehir Aytan
Bahceci Health Group, Istanbul, Turkey.
Cem
C
Celik
Department of Obstetrics and Gynecology, Istanbul University School of Medicine, Istanbul, Turkey.
Faruk
F
Buyru
Department of Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
John
J
Yeh
D000026:Abortion, Habitual / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008597:Menstrual Cycle / Q000097:blood / Q000378:metabolism; D018396:Mucin-1 / Q000097:blood / Q000378:metabolism / Q000502:physiology*
D000071816:Glycodelin; D006023:Glycoproteins; C060192:MUC1 protein, human; D018396:Mucin-1; C045246:PAEP protein, human
Glycodelin A; Mucin 1; infertility; recurrent implantation failure