Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus.
MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated from bovine mammary gland tissue samples from the S. aureus-induced mastitis group and controls. miRNAs were analyzed using Solexa sequencing and bioinformatics processing for the experimental group and control group. Two miRNA libraries were constructed respectively. A total of 370 known bovine miRNAs and 341 novel mi RNAs were detected for the S. aureus and 358 known bovine miRNAs and 232 novel miRNAs for control groups. A total of 77 miRNAs in the S. aureus group showed significant differences compared to the control group. GO (Gene Ontology) analysis showed these target genes were involved in the regulation of cells, binding, etc., while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in endocytosis, and olfactory transduction pathways involved in cancer. These results provide an experimental basis to reveal the cause and regulatory mechanism of mastitis and also suggest the potential of miRNAs to serve as biomarkers for the diagnosis of mastitis in dairy cows.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. lirui871025@126.com.
Rui
R
Li
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. yangdazhangcl@163.com.
Cheng-Long
CL
Zhang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. kevin89free@163.com.
Xiang-Xiang
XX
Liao
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. dan0513_2015@126.com.
Dan
D
Chen
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. 15371254521@163.com.
Wen-Qiang
WQ
Wang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. zhuyh2015@163.com.
Yi-Hui
YH
Zhu
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. xhgeng2012@163.com.
Xiao-Han
XH
Geng
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. djji@yzu.edu.cn.
De-Jun
DJ
Ji
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. cattle@yzu.edu.cn.
Yong-Jiang
YJ
Mao
The Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, Toronto, ON M5S 2J7, Canada. yunchen.gong@utoronto.ca.
Yun-Chen
YC
Gong
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. yzp@yzu.edu.cn.
Zhang-Ping
ZP
Yang
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification
D015415:Biomarkers; D004269:DNA, Bacterial; D035683:MicroRNAs