Computational and experimental studies of reassociating RNA/DNA hybrids containing split functionalities.
Recently, we developed a novel technique based on RNA/DNA hybrid reassociation that allows conditional activation of different split functionalities inside diseased cells and in vivo. We further expanded this idea to permit simultaneous activation of multiple different functions in a fully controllable fashion. In this chapter, we discuss some novel computational approaches and experimental techniques aimed at the characterization, design, and production of reassociating RNA/DNA hybrids containing split functionalities. We also briefly describe several experimental techniques that can be used to test these hybrids in vitro and in vivo.
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA.
Kirill A
KA
Afonin
Basic Science Program, Leidos Biomedical Research Inc., National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.
Eckart
E
Bindewald
Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, NCI, National Cancer Institute, Frederick, Maryland, USA.
Maria
M
Kireeva
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA. Electronic address: shapirbr@mail.nih.gov.
Bruce A
BA
Shapiro
D000465:Algorithms; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D020868:Gene Silencing; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011381:Programming Languages; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D034741:RNA, Small Interfering; D013816:Thermodynamics
D004798:Enzymes; D034741:RNA, Small Interfering; D012313:RNA; D004247:DNA
RNA/DNA hybrids; Specific gene silencing; Split functionalities; Thermodynamic of RNA/DNA hybrids reassociation; siRNA delivery