Primary characterization and evaluation of anti ulcerogenic activity of an aqueous extract from callus culture of Cereus peruvianus Mill. (Cactaceae).
In the current study we reported cultivation, extraction procedure, analysis and preliminary characterization of the aqueous extract from Cereus peruvianus callus culture and evaluated its anti ulcerogenic activity in vivo models of experimental ulcers in Wistar rats. The obtained aqueous extract from callus (AC) was dialyzed and subjected to freeze-thaw process, providing a possible polysaccharide. The carbohydrate and protein contents of the aqueous extract were estimated at 53.4% and 0.66%, respectively, composed primarily of galactose, arabinose and galacturonic acid, with minor amounts of glucose. This appeared heterogeneous when analyzed by high-performance size-exclusion chromatography and a multiangle laser light scattering detector (HPSEC-MALLS). The AC was found to be significantly effective against ethanol-induced lesions but was ineffective against indomethacin-induced lesions. The callus culture of C. peruvianus is an alternative source for the synthesis of substances originally produced by plants. The calluses grown indefinitely in vitro under controlled conditions are stable tissues, and the aqueous extract from calluses may be used instead of fully developed plants using the protocols described in this study.
Milena O
MO
Jayme
Franciele Q
FQ
Ames
Ciomar A
CA
Bersani-Amado
Maria de Fatima P S
Mde F
Machado
Claudete A
CA
Mangolin
Regina A C
RA
Goncalves
Departamento de Biotecnologia, Genetica e Biologia Celular, Universidade Estadual de Maringa, CEP 87.020-900 Maringa, PR, Brazil. mfpsmachado@uem.br.
Arildo J B
AJ
de Oliveira
D000818:Animals; D000897:Anti-Ulcer Agents / Q000032:analysis / Q000627:therapeutic use*; D029421:Cactaceae; D002241:Carbohydrates / Q000032:analysis; D000431:Ethanol; D007213:Indomethacin; D008297:Male; D010936:Plant Extracts / Q000032:analysis / Q000627:therapeutic use*; D010940:Plant Proteins / Q000032:analysis; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*
D000897:Anti-Ulcer Agents; D002241:Carbohydrates; D010936:Plant Extracts; D010940:Plant Proteins; D000431:Ethanol; D007213:Indomethacin