Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis.
OBJECTIVE
To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).


METHODS
Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 µL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.


RESULTS
All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.


CONCLUSIONS
The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.
Department of Prosthodontics, Faculty of Dentistry, İnönü University, Malatya, Turkey.
Meral Arslan
MA
Malkoç
Department of Prosthodontics, Faculty of Dentistry, İnönü University, Malatya, Turkey.
Necla
N
Demİr
Department of Restorative Dentistry, Faculty of Dentistry, Kırıkkale University, Kırıkkale, Turkey.
Abdulkadir
A
Şengün
Research Center, Faculty of Dentistry, Selçuk University, Konya, Turkey.
Şerife Buket
ŞB
Bozkurt
Department of Periodontology, Faculty of Dentistry, Selçuk University, Konya, Turkey.
Sema Sezgin
SS
Hakki
Cytotoxicity; Dental pulp-derived cells; Real-time cell analysis; Temporary cements