Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.
Protein-protein interactions (PPIs) are important for various biological processes in living cells. Several methods have been developed for the visualization of PPIs in vivo; however, these methods are unsuitable for visualization of post-PPI events such as dissociation and translocation. In this study, we applied a split SNAP-tag system for the visualization of post-PPI events. This method enabled tracking of the protein following dissociation from the protein-protein complex. Thus, the split SNAP-tag system should prove to be a useful tool for visualization of post-PPI events.
Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan. Electronic address: mie.m.aa@m.titech.ac.jp.
Masayasu
M
Mie
Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan.
Tatsuhiko
T
Naoki
Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan.
Eiry
E
Kobatake
D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D051571:Protein Kinase C-alpha / Q000378:metabolism
D019853:O(6)-Methylguanine-DNA Methyltransferase; D051571:Protein Kinase C-alpha
Complementation; Protein–protein interaction; SNAP-tag; Visualization