Antibody purification from human plasma by metal-chelated affinity membranes.
Immobilized metal ion affinity chromatography (IMAC) has been used for purification of proteins. IMAC introduces a new approach for selectively interacting biomolecules on the basis of their affinities for metal ions. The separation is based on different binding abilities of the proteins to the chelated metal ions on support. Here, N-methacryloyl-(L)-histidine methyl ester (MAH) is used as the metal-chelating ligand. Poly(hydroxyethyl methacrylate) Poly(HEMA) based membranes were prepared by photo-polymerization technique. Then, Zn(2+), Ni(2+), Co(2+), and Cu(2+) ions were chelated directly on the poly(HEMA-MAH) membranes for purification of immunoglobulin G (IgG) from human plasma.
Biochemistry Division, Department of Chemistry, Hacettepe University, Chemistry Department, Ankara, 06800, Turkey.
Handan
H
Yavuz
Nilay
N
Bereli
Fatma
F
Yılmaz
Canan
C
Armutcu
Adil
A
Denizli
D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006639:Histidine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D008567:Membranes, Artificial; D008670:Metals / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000737:chemistry; D058105:Polymerization; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013499:Surface Properties
D002614:Chelating Agents; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D008670:Metals; C486017:N-methacryloly-(L)-histidinemethylester; D011073:Polyamines; D013438:Sulfhydryl Compounds; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate; D006639:Histidine