Genome level analysis of bacteriocins of lactic acid bacteria.
Bacteriocins are antimicrobial peptides which are ribosomally synthesized by mainly all bacterial species. LABs (lactic acid bacteria) are a diverse group of bacteria that include around 20 genera of various species. Though LABs have a tremendous potential for production of anti-microbial peptides, this group of bacteria is still underexplored for bacteriocins. To study the diversity among bacteriocin encoding clusters and the putative bacteriocin precursors, genome mining was performed on 20 different species of LAB not reported to be bacteriocin producers. The phylogenetic tree of gyrB, rpoB, and 16S rRNA were constructed using MEGA6 software to analyze the diversity among strains. Putative bacteriocins operons identified were found to be diverse and were further characterized on the basis of physiochemical properties and the secondary structure. The presence of at least two cysteine residues in most of the observed putative bacteriocins leads to disulphide bond formation and provide stability. Our data suggests that LABs are prolific source of low molecular weight non modified peptides.
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India.
Neetigyata Pratap
NP
Singh
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India.
Abhay
A
Tiwari
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India.
Ankiti
A
Bansal
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India.
Shruti
S
Thakur
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India.
Garima
G
Sharma
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, India. Electronic address: reema.gabrani@jiit.ac.in.
Reema
R
Gabrani
D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D023281:Genomics; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007934:Leuconostoc / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D010802:Phylogeny; D017433:Protein Structure, Secondary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013291:Streptococcus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001430:Bacteriocins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid
BAGEL3; Bacteriocins; MEGA6; ProtParam; SOPMA