The long-term effects of FSH and triiodothyronine administration during the pubertal period on Connexin 43 expression and spermatogenesis efficiency in adult rats.
Follicle-stimulating hormone (FSH) and triiodothyronine (T3) are known regulatory factors of spermatogenesis initiation. Hyperstimulation of both hormones evokes regressional changes in connexin 43 expression and the seminiferous epithelium in young rats during testicular maturation. However, separate treatments with T3 reduce Sertoli cell number, which seems to be closely connected with the maturation of connexin 43 gap junctions. FSH elevates Sertoli cell number and function, but this effect may take place regardless of the presence of connexin 43-dependent intercellular communication. The aim of the study was to evaluate the later effects of such treatments. Newborn, male Wistar rats were divided randomly into experimental groups receiving daily subcutaneous injections of either 7.5 IU/animal FSH, or 100 mg/kg b.w. T3, or both substances or the same volume of vehicle (control group) until day 15 of life. The animals were sacrificed on day 50. Morphometric analysis and immunohistochemical reactions were performed using antibodies against Vimentin, Proliferating Cell Nuclear Antigen and Connexin 43 in the testis. Sertoli cell count, efficiency of spermatogenesis, and hormonal pattern were examined. Disturbances in the connexin 43 expression reduced the number of Sertoli cells, the efficiency of spermatogenesis and impaired endocrine function of testes in adult rats treated with FSH and T3 during puberty. Stimulation with FSH alone increased Sertoli cell number, but was associated with a negative effect on cell-to-cell connexin 43-dependent communication, with a consequential reduction of spermatogenesis efficiency. J. Exp. Zool. 323A: 256-265, 2015. © 2015 Wiley Periodicals, Inc.
Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
Katarzyna
K
Marchlewska
Jolanta
J
Slowikowska-Hilczer
Renata
R
Walczak-Jedrzejowska
Elzbieta
E
Oszukowska
Eliza
E
Filipiak
Krzysztof
K
Kula
D000818:Animals; D000831:Animals, Newborn; D018031:Connexin 43 / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D017629:Gap Junctions / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D012708:Sertoli Cells / Q000502:physiology; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D014284:Triiodothyronine / Q000494:pharmacology*
D018031:Connexin 43; D014284:Triiodothyronine; D005640:Follicle Stimulating Hormone