Reconstitution of a bioengineered salivary gland using a three-dimensional cell manipulation method.
One concept in regenerative therapy is the replacement of a lost or damaged organ with a regenerated, fully functional organ. Three-dimensional cell manipulation techniques, designated "organ germ methods," enable the normal development of a bioengineered organ germ in several types of ectodermal organs, such as teeth, hair follicles, and secretory glands. This method is useful for both organ regeneration technology and developmental biology, including cell kinetic analysis and the elucidation of gene regulation during organogenesis. Here, we describe a protocol for salivary gland reconstitution using the organ germ method to transplant a bioengineered salivary gland germ.
Organ Technologies Inc, Minato-ku, Tokyo, Japan.|RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan.
Miho
M
Ogawa
Organ Technologies Inc, Minato-ku, Tokyo, Japan.|RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan.
Takashi
T
Tsuji
D000818:Animals; D001698:Biomedical Engineering / Q000379:methods*; D004210:Dissection; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D008648:Mesoderm / Q000166:cytology; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D012469:Salivary Glands / Q000502:physiology* / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods*
bioengineered salivary gland; organ culture; organ germ method; regeneration; transplantation