Hematologic and immunological characteristics of Henoch-Schönlein purpura in rat and rabbit models induced with ovalbumin based on type III hypersensitivity.
Henoch-Schönlein purpura (HSP) is a common systemic vasculitis in children. Animal models of HSP are needed to better understand the mechanism of HSP. Here, we investigated hematologic and immunologic profiles in HSP rat and rabbit models. Models were established with ovalbumin (OVA) based on type III hypersensitivity. During the acute phase, the models exhibited varying degrees of cutaneous purpura, joint inflammatory response, gastrointestinal bleeding, glomerular capsule protein exudation, vascular dilatation, and increased IgA expression and immune complex deposition. Twenty four hours after antigen challenge, compared with the controls, the models showed a significantly increased white blood cell count and granulocytes count and percentage, decreased number and percentage of lymphocytes, no change in platelet concentration, significantly increased serum IL-4 and TNF-α levels, and decreased CD4(+) T cell, CD4/CD8 ratio, and C3 and C4 levels. Compared with the hematologic and immunologic profiles in pediatric HSP patients, the rat and rabbit HSP models can mimic pediatric HSP characteristics. Our studies provide two useful animal models for further investigations of the pathogenesis, diagnosis, drug screening and treatment of HSP.
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Yanhong
Y
Li
Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021.
Xiaochun
X
Feng
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Lan
L
Huang
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Hua
H
Zhu
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Yanfeng
Y
Xu
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Xiaolong
X
Sui
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Yuhuan
Y
Xu
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Yunlin
Y
Han
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China.
Chuan
C
Qin
D000818:Animals; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D011695:IgA Vasculitis / Q000097:blood / Q000139:chemically induced* / Q000276:immunology*; D007105:Immune Complex Diseases / Q000097:blood / Q000139:chemically induced / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010047:Ovalbumin; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D016207:Cytokines; D010047:Ovalbumin