ABCA1 expression in macrophages of allogeneic hematopoietic stem cell transplantation patients with severe infection undergoing continuous blood purification.
BACKGROUND
Excessive activation of the inflammatory mediator cascade after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients is associated with high mortality. Many studies have shown that continuous blood purification (CBP) could improve the prognosis of allo-HSCT patients with severe infection. However, the exact mechanism remains unclear. The aim of this study was to observe the effect of CBP on the expression of ATP-binding cassette transporter A1 (ABCA1) in macrophages, and to investigate the interventional effects of CBP on serum cytokine in allo-HSCT patients with severe infection.


METHODS
A total of 26 allo-HSCT patients with severe infection were included in this study. Before CBP and after CBP, blood samples were collected to observe hepatic and renal function, and the serum levels of TNF-α, IL-1, IL-6, and IL-10 were detected via ELISA. The THP-1 macrophages were exposed to serum samples obtained from patients at specific time points during CBP to test the changes of ABCA1 in macrophages by real-timePCR and Western blotting.


RESULTS
Serum creatinine, alanine aminotransferase, and C reaction protein (CRP) levels decreased significantly after CBP. Moreover, TNF-α, IL-1, and IL-6 serum levels decreased significantly, but IL-10 level increased significantly after CBP (P<.05). After CBP, ABCA1 expression levels were higher than those before CBP, and ABCA1 expression was significantly increased with the supplementation of CBP (P<.05).


CONCLUSIONS
CBP improved the condition of allo-HSCT patients with severe infection. CBP may be a potent up-regulator of the ABCA1 levels in macrophages of allo-HSCT patients with severe infection.
1 Department of Hematology, the Third People's Hospital of Zhengzhou, Zhengzhou - China.
Xiu-Juan
XJ
Dong
Zhi-Min
ZM
Zhang
Xiao-Wu
XW
Zhao
Fan
F
Wu
D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D016207:Cytokines / Q000097:blood; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006440:Hemofiltration; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008297:Male; D012720:Severity of Illness Index; D013997:Time Factors; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D015854:Up-Regulation; D055815:Young Adult
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators