Analysis of FHIT gene methylation in egyptian breast cancer women: association with clinicopathological features.
BACKGROUND
Fragile histidine triad (FHIT) gene is a tumor suppressor gene which involved in breast cancer pathogenesis. Epigenetics alterations in FHIT contributes to tumorigenesis of breast cancer.


OBJECTIVE
Our objective was to study FHIT promoter region hypermethylation in Egyptian breast cancer patients and its association with clinicopathological features.


METHODS
Methylation-specific polymerase chain reaction was performed to study the hypermethylation of FHIT promoter region in 20 benign breast tissues and 30 breast cancer tissues.


RESULTS
The frequency of hypermethylation of FHIT promoter region was significantly increased in breast cancer patients compared to bengin breast disease patients. The Odd ?s ratio (95%CI) of development of breast cancer in individuals with FHIT promoter hypermethylation (MM) was 11.0 (1.22-250.8). There were also significant associations between FHIT promoter hypermethylation and estrogen, progesterone receptors negativity, tumor stage and nodal involvment in breast cancer pateints.


CONCLUSIONS
Our results support an association between FHIT promotor hypermethylation and development of breast cancer in Egyptian breast cancer patients. FHIT promoter hypermethylation is associated with some poor prognostic features of breast cancer.
Clinical Pathology, Faculty of Medicine, Zagazig University Hospitals, Zagazig, Egypt E-mail : dr.marwa_samir@yahoo.com.
Seham Mahrous
SM
Zaki
Hala A
HA
Abdel-Azeez
Mona Roshdy
MR
El Nagar
Khaled Abdel-Aziz
KA
Metwally
Marwa M Samir
MM
S Ahmed
D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000556:secondary*; D018275:Carcinoma, Lobular / Q000235:genetics / Q000556:secondary*; D016022:Case-Control Studies; D019175:DNA Methylation; D004534:Egypt; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase