The presence of variant genotype of the mannose-binding lectin gene (MBL2) is not associated with increased restenosis rate in carotid surgery.
BACKGROUND
We investigated the role of mannose-binding lectin (MBL) variant genotypes in patients with significant carotid restenosis after carotid endarterectomy (CEA) and who underwent a revision CEA.


METHODS
This was a cross-sectional analysis of 97 patients enrolled between 2001 and 2013. Three groups were investigated: group A included patients with internal carotid artery restenosis, group B included patients without restenosis after CEA, and group C included patients with peripheral arterial disease but without any signs of a carotid stenosis. Venous blood samples were drawn for the genotyping for MBL2 by polymerase chain reaction and for the determination of the MBL serum concentration by enzyme-linked immunoabsorbent assay.


RESULTS
The serum concentration of MBL was higher in patients with the normal genotype than in those with the genotype variants of MBL (95% confidence interval, 272.8-1008.7 μg/L; P = .001). There was no statistically significant difference among groups A, B, or C with respect to the presence of a variant genotype. Similarly, there was no significant gender difference regarding the presence of a variant genotype (P = .325).


CONCLUSIONS
The presence of a variant genotype of the MBL2 gene (and the correspondingly lower serum concentration of this molecule) was not correlated with the development of carotid restenosis after CEA beyond a follow-up of 12 months.
Department of Vascular and Endovascular Surgery-University Hospital of Cologne, Cologne, Germany. Electronic address: waelsahmad@gmail.com.
Wael
W
Ahmad
Department of Vascular and Endovascular Surgery-University Hospital of Cologne, Cologne, Germany.
Payman
P
Majd
Department of Vascular and Endovascular Surgery-University Hospital of Cologne, Cologne, Germany.
Thomas
T
Lübke
Division of Vascular Surgery, Merheim Clinical Center, Cologne, Germany.
Marco
M
Aleksic
Department of Vascular and Endovascular Surgery-University Hospital of Cologne, Cologne, Germany.
Jan Sigge
JS
Brunkwall
D000368:Aged; D016893:Carotid Stenosis / Q000235:genetics / Q000601:surgery*; D016894:Endarterectomy, Carotid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012008:Recurrence
C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin