Proteomic Analysis of Erythritol-Producing Yarrowia lipolytica from Glycerol in Response to Osmotic Pressure.
Osmotic pressure is a critical factor for erythritol production with osmophilic yeast. Protein expression patterns of an erythritol-producing yeast, Yarrowia lipolytica, were analyzed to identify differentially-expressed proteins in response to osmotic pressure. In order to analyze intracellular protein levels quantitatively, two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Y. lipolytica cultured under low (3.17 osmol/kg) and high (4.21 osmol/kg) osmotic pressures. Proteomic analyses allowed identification of 54 differentially-expressed proteins among the proteins distributed in the range of pI 3-10 and 14.4-97.4 kDa molecular mass between the osmotic stress conditions. Remarkably, the main proteins were involved in the pathway of energy, metabolism, cell rescue, and stress response. The expression of such enzymes related to protein and nucleotide biosynthesis was inhibited drastically, reflecting the growth arrest of Y. lipolytica under hyperosmotic stress. The improvement of erythritol production under high osmotic stress was due to the significant induction of a range of crucial enzymes related to polyols biosynthesis, such as transketolase and triosephosphate isomerase, and the osmotic stress responsive proteins like pyridoxine-4-dehydrogenase and the AKRs family. The polyols biosynthesis was really related to an osmotic response and a protection mechanism against hyperosmotic stress in Y. lipolytica. Additionally, the high osmotic stress could also induce other cell stress responses as with heat shock and oxidation stress responses, and these responsive proteins, such as the HSPs family, catalase T, and superoxide dismutase, also had drastically increased expression levels under hyperosmotic pressure.
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Li-Bo
LB
Yang
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Xiao-Meng
XM
Dai
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Zhi-Yong
ZY
Zheng
Jiangsu Rayguang Biotechnology Co., Ltd., Wuxi, Jiangsu 214125, P.R. China.
Li
L
Zhu
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Xiao-Bei
XB
Zhan
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Chi-Chung
CC
Lin
D015180:Electrophoresis, Gel, Two-Dimensional; D004896:Erythritol / Q000378:metabolism*; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism*; D007526:Isoelectric Point; D053858:Metabolic Networks and Pathways; D008970:Molecular Weight; D009997:Osmotic Pressure; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D013312:Stress, Physiological; D025062:Yarrowia / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*
D005656:Fungal Proteins; D020543:Proteome; D005990:Glycerol; D004896:Erythritol
Erythritol; Osmotic stress response; Proteomics; Yarrowia lipolytica