Activated expression of AtEDT1/HDG11 promotes lateral root formation in Arabidopsis mutant edt1 by upregulating jasmonate biosynthesis.
Root architecture is crucial for plants to absorb water and nutrients. We previously reported edt1 (edt1D) mutant with altered root architecture that contributes significantly to drought resistance. However, the underlying molecular mechanisms are not well understood. Here we report one of the mechanisms underlying EDT1/HDG11-conferred altered root architecture. Root transcriptome comparison between the wild type and edt1D revealed that the upregulated genes involved in jasmonate biosynthesis and signaling pathway were enriched in edt1D root, which were confirmed by quantitative RT-PCR. Further analysis showed that EDT1/HDG11, as a transcription factor, bound directly to the HD binding sites in the promoters of AOS, AOC3, OPR3, and OPCL1, which encode four key enzymes in JA biosynthesis. We found that the jasmonic acid level was significantly elevated in edt1D root compared with that in the wild type subsequently. In addition, more auxin accumulation was observed in the lateral root primordium of edt1D compared with that of wild type. Genetic analysis of edt1D opcl1 double mutant also showed that HDG11 was partially dependent on JA in regulating LR formation. Taken together, overexpression of EDT1/HDG11 increases JA level in the root of edt1D by directly upregulating the expressions of several genes encoding JA biosynthesis enzymes to activate auxin signaling and promote lateral root formation.
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Xiao-Teng
XT
Cai
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Ping
P
Xu
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Yao
Y
Wang
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Cheng-Bin
CB
Xiang
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism*; D005453:Fluorescence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics*
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C586313:HDG11 protein, Arabidopsis; D007210:Indoleacetic Acids; D054883:Oxylipins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; C011006:jasmonic acid
EDT1/HDG11; OPCL1; OPR3; jasmonic acid; lateral root