Genotyping and characterization of CTX-M-15 -producing Klebsiella pneumoniae isolated from an Iranian hospital.
The aims were to describe the genetic characterization of blaCTX-M-1 group gene in Klebsiella pneumoniae and to investigate the relationship between isolates by MLVA and PFGE. We analyzed 36 CTX-M group 1-ESBL producing K. pneumoniae. rmpA and wcaG virulence genes were identified by PCR. The genetic environment of blaCTX-M-1 was analyzed by PCR and sequencing. Plasmid replicons were determined using PCR-based replicon typing. The isolates were typed by MLVA and PFGE. All blaCTX-M-1 were blaCTX-M-15. The wcaG and rmpA were detected in 1 and 2 isolates, respectively. IncF were the most frequently detected replicons (63.88%). In all isolates, ISEcp1 was found upstream and orf477 downstream of blaCTX-M-15, IS26 was found in two isolates. MLVA identified 20 MLVA types, whereas PFGE identified 25 different profiles. The dissemination of CTX-M-15 in our isolates was due to the clonal spread of isolates and to the genetic transfer of mobile elements among unrelated strains.
a Department of Bacteriology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Safoura
S
Derakhshan
a Department of Bacteriology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Shahin Najar
SN
Peerayeh
a Department of Bacteriology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Bita
B
Bakhshi
D024881:Drug Resistance, Bacterial; D060005:Genotyping Techniques; D006761:Hospitals; D007492:Iran; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012093:Replicon; D037521:Virulence Factors / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D037521:Virulence Factors; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases
CTX-M-15 β-lactamase; IncF; Klebsiella pneumoniae; MLVA