The effects of ROS in prostatic stromal cells under hypoxic environment.
OBJECTIVE
The objective of this study is to explore the effects of reactive oxygen species (ROS) under hypoxic environment in prostatic stromal cells (PSC).


METHODS
To detect the expression of ROS in PSC and the tissues of benign prostatic hyperplasia (BPH) by flow cytometry; under hypoxic conditions, to observe the changes of cells growth and ROS in PSC; quantitative PCR was used to detect hypoxia inducible factor-1α (HIF-1α), androgen receptors (AR), vascular endothelial growth factor (VEGF), and interleukin-8 (IL-8) in PSC; After edaravone intervening, to examine the changes of cells growth, ROS, HIF-1α, AR, VEGF, and IL-8 under hypoxic conditions.


RESULTS
The expression of ROS in tissues and cells which under hypoxic condition was significantly increased. 3% O2 promoted the proliferation. The HIF-1α, AR, VEGF, and IL-8 were upregulated under 3% O2. After edaravone intervening, ROS significantly decreased, HIF-1α and VEGF were downregulated, and cell proliferation declined.


CONCLUSIONS
Hypoxia stimulates the generation of ROS, and the ROS may play a key role in BPH.
Department of Urology, Affiliated Hospital of Qinghai University , Xining , PR China and.
Hailin
H
Ren
Xiaona
X
Li
Guojun
G
Cheng
Ning
N
Li
Zhi
Z
Hou
Jiming
J
Suo
Jian
J
Wang
Xi
X
Za
D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C508606:CXCL8 protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D011944:Receptors, Androgen; D042461:Vascular Endothelial Growth Factor A
AR; BPH; HIF-1α; IL-8; ROS; VEGF; edaravone; hypoxia