Inhibition of adhesion of Clostridium difficile to human intestinal cells after treatment with serum and intestinal fluid isolated from mice immunized with nontoxigenic C. difficile membrane fraction.
Diarrhea and pseudomembrane colitis caused by Clostridium difficile infection is a global health concern because of the high recurrence rate after standard antibiotic therapy. Vaccination presents a powerful countermeasure against disease recurrence. In this study, mice vaccinated with the nontoxigenic C. difficile membrane fraction generated a marked immune response to the antigen, as demonstrated by the serum IgG and intestinal fluid IgA levels. Significantly, pretreatment with harvested IgG- and IgA-containing fluids was sufficient to prevent in vitro adhesion of C. difficile to human Caco-2 intestinal cells. These results highlight the potential of nontoxigenic C. difficile membrane fraction as a vaccine candidate for C. difficile infection.
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan. Electronic address: senoh@niid.go.jp.
Mitsutoshi
M
Senoh
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Masaaki
M
Iwaki
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Akihiko
A
Yamamoto
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Haru
H
Kato
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Tadashi
T
Fukuda
National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Keigo
K
Shibayama
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001422:Bacterial Adhesion / Q000187:drug effects; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D018938:Caco-2 Cells; D016360:Clostridioides difficile / Q000276:immunology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D014611:Vaccination / Q000379:methods*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G
Adhesion; Clostridium difficile; Membrane fraction; Vaccine