Detection of enterovirus D68 in Canadian laboratories.
The recent emergence of a severe respiratory disease caused by enterovirus D68 prompted investigation into whether Canadian hospital and provincial laboratories can detect this virus using commercial and laboratory-developed assays. This study demonstrated analytical sensitivity differences between commercial and laboratory-developed assays for the detection of enterovirus D68.
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Dalhousie University, Halifax, Nova Scotia, Canada Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada, University of Alberta, Edmonton, Alberta, Canada.
Todd F
TF
Hatchette
Provincial Laboratory for Public Health (ProvLab), University of Alberta, Edmonton, Alberta, Canada Department Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
Steven J
SJ
Drews
Enterovirus and Enteric Virus Laboratory, National Microbiology Laboratory (NML), Winnipeg, Manitoba, Canada.
Elsie
E
Grudeski
Enterovirus and Enteric Virus Laboratory, National Microbiology Laboratory (NML), Winnipeg, Manitoba, Canada.
Tim
T
Booth
Laboratoire de Santé Publique du Québec (LSPQ)/Institut National de Santé Publique du Québec (INSPQ), Québec, Canada.
Christine
C
Martineau
Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
Kerry
K
Dust
Centre Hospitalier Universitaire George L. Dumont, Moncton, New Brunswick, Canada.
Richard
R
Garceau
Public Health Ontario and University of Toronto, Toronto, Ontario, Canada.
Jonathan
J
Gubbay
EORLA Regional Virology Laboratory, Children's Hospital of Eastern Ontario (CHEO), University of Ottawa, Ottawa, Ontario, Canada.
Tim
T
Karnauchow
Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
Mel
M
Krajden
Saskatchewan Disease Control Lab (SDCL), Ministry of Health, Regina, Saskatchewan, Canada.
Paul N
PN
Levett
Department of Microbiology, Mount Sinai Hospital, University Health Network, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Tony
T
Mazzulli
Saskatchewan Disease Control Lab (SDCL), Ministry of Health, Regina, Saskatchewan, Canada.
Ryan R
RR
McDonald
Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
Alan
A
McNabb
Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
Samira
S
Mubareka
Public Health Laboratory and Microbiology, St-John's, Newfoundland, Canada.
Robert
R
Needle
The Hospital for Sick Children, Toronto, Ontario, Canada.
Astrid
A
Petrich
The Hospital for Sick Children, Toronto, Ontario, Canada.
Susan
S
Richardson
St-Joseph's Healthcare, Hamilton, Ontario, Canada.
Candy
C
Rutherford
St-Joseph's Healthcare, Hamilton, Ontario, Canada.
Marek
M
Smieja
Provincial Laboratory for Public Health (ProvLab), University of Calgary, Calgary, Alberta, Canada.
Raymond
R
Tellier
Provincial Laboratory for Public Health (ProvLab), University of Alberta, Edmonton, Alberta, Canada Medical Microbiology and Immunology University of Alberta, Edmonton, Alberta, Canada.
Graham
G
Tipples
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Dalhousie University, Halifax, Nova Scotia, Canada Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada, University of Alberta, Edmonton, Alberta, Canada jason.leblanc@cdha.nshealth.ca.
Jason J
JJ
LeBlanc
D002170:Canada; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D030016:Enterovirus D, Human / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D059021:Laboratory Proficiency Testing; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity