Lead acetate-induced hepatoxicity in Wistar rats: possible protective role of combination therapy.
Experimental studies have shown that sulphur-containing antioxidants have beneficial effects against the detrimental properties of Pb. In the present study, we investigated the therapeutic potential of combined administration of N-acetyl cysteine (NAC; 50 mg/kg p.o.) and selenium (Se; 0.5 mg/kg p.o.) against lead acetate intoxication (Pb; 0.2% in water ad libitum) in a male rat model for 12 wk. The exposure of rats to lead acetate produced significant (P < 0.05) alterations in cytochrome P450 (CYP) activity, microsomal lipid peroxidation, reduced glutathione, and proteins. In addition, significant elevation in liver markers transaminases, triglycerides, cholesterol, and bilirubin as well as a decline in albumin were also compared with the experimental control rats. Combined treatment of lead-exposed animals with NAC and Se showed marked improvement of the biochemical, molecular, and histopathological findings. These experimental results strongly indicate the protective effect of NAC alone with Se against toxic effects of lead on liver tissue.
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research and School of Studies in Zoology, Jiwaji University, Gwalior (MP) India.
Samta
S
Sharma
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research and School of Studies in Zoology, Jiwaji University, Gwalior (MP) India.
Suchita
S
Raghuvanshi
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research and School of Studies in Zoology, Jiwaji University, Gwalior (MP) India.
Amita
A
Jaswal
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research and School of Studies in Zoology, Jiwaji University, Gwalior (MP) India.
Sadhana
S
Shrivastava
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research, and School of Studies in Zoology, Jiwaji University, Gwalior (M.P.), India.
Sangeeta
S
Shukla
D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004249:DNA Damage; D004359:Drug Therapy, Combination; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*
D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; D000251:Adenosine Triphosphatases; D012643:Selenium; C008261:lead acetate; D000111:Acetylcysteine