Genetic variability analysis of Zymomonas mobilis strains from the UFPEDA microorganisms collection.
Zymomonas mobilis is a Gram-negative bacterium that has drawn attention in the bioethanol industry. Besides bioethanol, this bacterium also produces other biotechnological products such as levans, which show antitumor activity. Molecular studies involving Z. mobilis have advanced to the point that allows us to characterize interspecies genetic diversity and understand their metabolism, and these data are essential for better utilization of this species. In this study, the genetic diversity of 24 strains from the Microorganisms Collection of Departamento de Antibióticos (UFPEDA) from Universidade Federal de Pernambuco were characterized. The methods used were amplified ribosomal DNA restriction analysis and diversity analysis of the internally transcribed 16S-23S rDNA spacer region (ISR). These analyses revealed low genetic variability of the 16S rDNA gene. These data confirm that these isolates are, or are closely related to, Z. mobilis. Moreover, the analysis of the ISR confirmed the genetic variability of strains deposited in the UFPEDA collection of microorganisms and grouped these strains into ten ribotypes, which can be used in the future for breeding programs and for the preservation of biodiversity. Furthermore, this study characterized the genetic variability between the UFPEDA 205/ ZAP, UFPEDA 98/AG11, and ZAG strains, which were obtained by spheroplast fusion among them. The data also indicate that there is genetic variability among the UFPEDA 202/CP4 and UFPEDA 633/ ZM4 strains, demonstrating that these important Z. mobilis strains are distinct, as suggested in previous studies.
Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil.
L C N
LC
Silva
Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil.
J M
JM
Araújo
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brasil.
J L
JL
Azevedo
Departamento de Biofísica e Radiobiologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil.
R J S A
RJ
Padilha
Departamento de Engenharia Biomédica, Centro de Tecnologia e Geociências, Universidade Federal de Pernambuco, Recife, PE, Brasil ricardo.yara@ufpe.br.
R
R
Yara
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D056804:Biofuels / Q000382:microbiology; D004275:DNA, Ribosomal / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism; D014644:Genetic Variation; D017355:Zymomonas / Q000235:genetics* / Q000378:metabolism
D056804:Biofuels; D004275:DNA, Ribosomal; D000431:Ethanol