[Preparation and identification of monoclonal antibody against repression and activation protein 1].
OBJECTIVE
To prepare and identify monoclonal antibody (mAb) against human telomere-associated repression and activation protein 1 (Rap1).


METHODS
BALB/c mice were immunized with recombinant Rap1 protein. The spleen cells of the immunized mice were fused with Sp2/0 cells. The positive clones were confirmed and selected by indirect ELISA for titer determination and specificity identification.


RESULTS
One hybridoma cell strain secreting specific mAb against Rap1 was obtained, namely 6F11. ELISA showed that the titer of the ascites was 1:10 000. Western blotting, immunoprecipitation and immunofluorescence experiments demonstrated that the mAb could specifically recognize and bind Rap1.


CONCLUSIONS
We have prepared mAb against human telomere-associated protein Rap1. The Rap1 mAb has a good binding ability and specificity.
Ministry of Education Key Laboratory of Carcinogenesis and Translational Research, Laboratory of Biochemistry and Molecular Biology, Cancer Hospital & Beijing Institute for Cancer Research, Peking University, Beijing 100142, China.
Lin
L
Meng
Ministry of Education Key Laboratory of Carcinogenesis and Translational Research, Laboratory of Biochemistry and Molecular Biology, Cancer Hospital & Beijing Institute for Cancer Research, Peking University, Beijing 100142, China.
Chengchao
C
Shou
Ministry of Education Key Laboratory of Carcinogenesis and Translational Research, Laboratory of Biochemistry and Molecular Biology, Cancer Hospital & Beijing Institute for Cancer Research, Peking University, Beijing 100142, China.
Shenyi
S
Lian
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D047468:Immunoprecipitation; D051379:Mice; D008807:Mice, Inbred BALB C; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000089804:Shelterin Complex; C440809:TERF2IP protein, human; D034501:Telomere-Binding Proteins