Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos.
It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels (except at the 2-cell stage). This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early pre-implantation mouse embryos.
School of Life Science, Anhui University, Hefei 230601, China;School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China.
Fang
F
Ding
School of Life Science, Anhui University, Hefei 230601, China;School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China.
Li
L
Chen
School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China;Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang 236037, China.
Yong
Y
Liu
School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China;Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang 236037, China.
Feng-Rui
FR
Wu
School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China;Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang 236037, China.
Biao
B
Ding
Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang 236037, China.
Wen-Yong
WY
Li
School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China. wangrbnu@aliyun.com.
Rong
R
Wang
D000107:Acetylation; D000818:Animals; D047108:Embryonic Development / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy
D006657:Histones; D000431:Ethanol
Alcohol intake; Embryo development; H3K9 acetylation; Mice; Pre-implantation embryos