Antibacterial activity of chitosan nanofiber meshes with liposomes immobilized releasing gentamicin.
Chitsan (Ch) nanofiber mesh (NFM) is a material with natural characteristics favoring its use in human wound dressing. The present work proposes a gentamicin-loaded liposome immobilized at the surface of Ch NFMs to promote its antibacterial activity. To achieve this purpose, Ch NFMs were functionalized with thiol groups, and gentamicin-loaded liposomes were covalently immobilized by the reaction of the SH groups with maleimide. The maximum concentration of SH groups (55.52±11.19nmolcm(-2)) was obtained at pH 7. A fluorescent dye was covalently bound to the SH groups present at the surface of electrospun Ch NFMs. Their spatial distribution was uniform throughout the NFMs when analyzed by fluorescence microscopy. Gentamicin was successfully encapsulated into the liposomes with an efficiency of 17%. Gentamicin-loaded liposomes were uniformly distributed at the surface of the Ch NFMs and the drug release kinetic showed a sustained release of gentamicin during 16h, achieving a steady state at 24h. The in vitro susceptibility tests confirmed that the gentamicin released from the liposomes immobilized at the surface of electrospun Ch NFM has bactericidal activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results show that the developed system has promising performance for wound dressing applications, avoiding infections caused by these common pathogens.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal. Electronic address: nelson.monteiro@gmail.com.
Nelson
N
Monteiro
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal; IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal. Electronic address: margarida.martins@dep.uminho.pt.
Margarida
M
Martins
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal. Electronic address: amartins@dep.uminho.pt.
Albino
A
Martins
CNC - Center for Neurosciences and Cell Biology, FFUC - Faculty of Pharmacy and University of Coimbra, 3000 Coimbra, Portugal. Electronic address: nuno.a.c.fonseca@gmail.com.
Nuno A
NA
Fonseca
CNC - Center for Neurosciences and Cell Biology, FFUC - Faculty of Pharmacy and University of Coimbra, 3000 Coimbra, Portugal. Electronic address: jmoreira@ff.uc.pt.
João N
JN
Moreira
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal. Electronic address: rgreis@dep.uminho.pt.
Rui L
RL
Reis
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal. Electronic address: nuno@dep.uminho.pt.
Nuno M
NM
Neves
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D055672:Static Electricity
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D008081:Liposomes; D048271:Chitosan
Chitosan; Drug delivery; Gentamicin; Liposomes; Wound dressing