In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates.
The in situ one-pot synthesis of peptide-polymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Jennifer
J
Collins
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Joji
J
Tanaka
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Paul
P
Wilson
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Kristian
K
Kempe
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Thomas P
TP
Davis
Michelle P
MP
McIntosh
Michael R
MR
Whittaker
†Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
David M
DM
Haddleton
D000180:Acrylic Resins / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D005260:Female; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D008301:Maleimides / Q000737:chemistry*; D010120:Oxytocics / Q000737:chemistry*; D010121:Oxytocin / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D055550:Protein Stability; D012996:Solutions
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D004220:Disulfides; D005609:Free Radicals; D008301:Maleimides; D010120:Oxytocics; D010636:Phenols; D012996:Solutions; C574088:poly(methoxy-polyethylene glycol acrylate); C043592:maleimide; D011092:Polyethylene Glycols; D010121:Oxytocin