A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei.
Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.
Advanced Enzyme Technologies Ltd., 5th Floor, A WING, Sun Magnetica, LIC Service Road, Louiswadi, Thane (W), 400 604, Maharashtra, India, dina@advancedenzymes.com.
Dina B
DB
Saroj
Shrinivas N
SN
Dengeti
Supriya
S
Aher
Anil K
AK
Gupta
D017931:DNA Primers / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014242:Trichoderma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D020648:Peptide Elongation Factor 1