Synthesis of multifunctional cellulose nanocrystals for lectin recognition and bacterial imaging.
Multifunctional cellulose nanocrystals have been synthesized and applied as a new type of glyconanomaterial in lectin binding and bacterial imaging. The cellulose nanocrystals were prepared by TEMPO-mediated oxidation and acidic hydrolysis, followed by functionalization with a quinolone fluorophore and carbohydrate ligands. The cellulose nanocrystals were subsequently applied in interaction studies with carbohydrate-binding proteins and in bacterial imaging. The results show that the functional cellulose nanocrystals could selectively recognize the corresponding cognate lectins. In addition, mannosylated nanocrystals were shown to selectively interact with FimH-presenting E. coli, as detected by TEM and confocal fluorescence microscopy. These glyconanomaterials provide a new application of cellulose nanocrystals in biorecognition and imaging.
Juan
J
Zhou
Núria
N
Butchosa
§Department of Chemistry, University of Massachusetts-Lowell, 1 University Avenue, Lowell, Massachusetts 01854, United States.
H Surangi N
HS
Jayawardena
§Department of Chemistry, University of Massachusetts-Lowell, 1 University Avenue, Lowell, Massachusetts 01854, United States.
JaeHyeung
J
Park
⊥School of Biotechnology, AlbaNova University Center, KTH - Royal Institute of Technology, S-10691 Stockholm, Sweden.
Qi
Q
Zhou
§Department of Chemistry, University of Massachusetts-Lowell, 1 University Avenue, Lowell, Massachusetts 01854, United States.
Mingdi
M
Yan
Olof
O
Ramström
D002482:Cellulose / Q000737:chemistry*; D004926:Escherichia coli / Q000648:ultrastructure*; D037102:Lectins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding
D037102:Lectins; D002482:Cellulose