Glucose tolerance tests for systematic screening of glucose homeostasis in mice.
This article presents a detailed description of intraperitoneal and oral glucose tolerance tests in mice. The former is widely used in initial high-throughput phenotyping of mutant mice to assess a diabetic phenotype and alterations in glucose homeostasis. Each protocol provides a comprehensive description of each step in the workflow, including variation of the standard protocol under particular circumstances (e.g., sensitivity to food deprivation, excessive deviations in body composition, or need for extra blood samples for additional analyses). We also describe how reduction of body mass and body temperature can be used as additional readouts to monitor metabolic function in response to food deprivation.
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.|These authors contributed equally to this work.
Jan
J
Rozman
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.|Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.|These authors contributed equally to this work.
Birgit
B
Rathkolb
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.|These authors contributed equally to this work.
Susanne
S
Neschen
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.
Helmut
H
Fuchs
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.
Valérie
V
Gailus-Durner
Molecular Nutritional Medicine, Else-Kröner Fresenius Center for Food Sciences & ZIEL-Research Center for Nutrition and Food Sciences, Technische Universität München, Freising, Germany.
Martin
M
Klingenspor
Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.
Eckhard
E
Wolf
Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.|German Research Center for Diabetes Research, Neuherberg, Germany.|Institute of Experimental Genetics, Life and Food Science Center Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.|Corresponding author: Martin Hrabě de Angelis (hrabe@helmholtz-muenchen.de).
Martin
M
Hrabě de Angelis
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis; D005951:Glucose Tolerance Test; D006706:Homeostasis; D006801:Humans; D051379:Mice / Q000502:physiology*; D023421:Models, Animal
D001786:Blood Glucose
IPGTT; OGTT; glucose intolerance; glucose tolerance test; high-throughput phenotyping; mouse models; thermovision; type 2 diabetes mellitus