Biodegradable hydrophilic polyurethane PEGU25 loading antimicrobial peptide Bmap-28: a sustained-release membrane able to inhibit bacterial biofilm formation in vitro.
Catheter-related infection makes up a large part of hospital infection and contributes 80% to all nosocomial urological infection, costing hundreds of millions dollar per year for treatment. Biodegradable hydrophilic material incorporating antibiotic substance is a promising way to prevent catheter-related infection. And antimicrobial peptide seems an optimal drug for its desirable antibiotic effect. In the current research, we produced a new kind of antibiotic material by incorporating antimicrobial peptide Bmap-28 with polyurethane PEGU25 and tested its effect on Proteus mirabilis in vitro. Compared with the control group, PEGU25 membrane incorporating Bmap-28 had a significant lower bacteria load after co-cultured with the Proteus mirabilis. And its antibiotic effect could be observed throughout the whole 7-day test. Also the Bmap-28 membrane could delay catheter obstruction caused by encrustation. Our findings reveal that PEGU25 incorporating Bmap-28 can well inhibit bacterial biofilm formation of common pathogens for catheter-related urinary tract infection in vitro, which makes it a promising antibiotic material for medical tubes for urology.
1] Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China [2] Department of Urology of the First Affiliated Hospital of Anhui Medical University, Hefei. 230032, China.
Jianzhong
J
Wang
Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China.
Qinyu
Q
Liu
Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China.
Ye
Y
Tian
Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China.
Zhongyu
Z
Jian
Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China.
Hong
H
Li
Department of Urology of West China Hospital, Sichuan University, Chengdu. 610041, China.
Kunjie
K
Wang
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000633:toxicity; D000890:Anti-Infective Agents; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D015169:Colony Count, Microbial; D003692:Delayed-Action Preparations; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011140:Polyurethanes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D011513:Proteus mirabilis / Q000187:drug effects / Q000502:physiology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C102360:BMAP-28; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D011140:Polyurethanes; D011506:Proteins