Utility of recombinant fragment C for assessment of anti-tetanus antibodies in plasma.
Anti-tetanus antibodies in biological samples are typically detected using an enzyme-linked immunosorbent assay based on toxoided tetanus neurotoxin as antigen. We demonstrate that recombinantly produced fragment C of the toxin heavy chain is an effective alternative antigen for assessment of tetanus-immune status in plasma samples.
Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville VA, USA.
Girija
G
Ramakrishnan
TechLab Inc., Blacksburg, VA, USA.
Karl
K
Pedersen
TechLab Inc., Blacksburg, VA, USA.
Denis
D
Guenette
TechLab Inc., Blacksburg, VA, USA.
Joyce
J
Sink
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
Rashidul
R
Haque
Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville VA, USA.
William A
WA
Petri
TechLab Inc., Blacksburg, VA, USA.
Joel
J
Herbein
Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville VA, USA. Electronic address: cg2p@virginia.edu.
Carol A
CA
Gilchrist
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D000992:Antitoxins / Q000097:blood*; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007223:Infant; D010949:Plasma / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D013742:Tetanus / Q000276:immunology* / Q000517:prevention & control*; D013744:Tetanus Toxin / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000992:Antitoxins; D011994:Recombinant Proteins; D013744:Tetanus Toxin
ELISA; Fragment C; Immune response; Recombinant antigen; Tetanus; Vaccine surveillance