Desorption electrospray ionization-based imaging of interaction between vascular graft and human body.
The desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) is known as a fast and convenient MS-based method for lipid imaging in various biological materials. Here, we applied this technique to visualize lipid distribution in a vascular graft removed from a patient's body. This is a good example of the DESI system capabilities toward imaging of interaction between artificial material and living tissues. Detailed analysis allowed for visualization of the spatial distribution of selected lipids in this implanted, artificial material. Not only DESI-MSI allowed visualization of lipid distribution in the investigated material but also enabled identification of the detected molecular species using MS/MS. Here, this technique was successfully used to evaluate the saturation and spatial distribution of endogenous lipids in the artificial vascular graft. Unambiguous identification of the lipids was done with the aid of fragmentation procedure. We also showed that various lipids localize preferably in graft material or internal plaque existing inside the graft.
Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, 30-059, Krakow, Poland.
Anna
A
Bodzon-Kulakowska
Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, 30-059, Krakow, Poland.
Anna
A
Drabik
Department of Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Bialystok University of Technology, 15-351, Bialystok, Poland.
Joanna
J
Mystkowska
Department of Vascular Surgery and Transplantology, Medical University of Bialystok, 15-089, Białystok, Poland.
Michal
M
Chlabicz
Department of Vascular Surgery and Transplantology, Medical University of Bialystok, 15-089, Białystok, Poland.
Marek
M
Gacko
Department of Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Bialystok University of Technology, 15-351, Bialystok, Poland.
Jan R
JR
Dabrowski
Academic Centre for Materials and Nanotechnology (ACMiN), AGH University of Science and Technology, 30-059, Krakow, Poland.
Przemyslaw
P
Mielczarek
Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, 30-059, Krakow, Poland.|Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-819, Zabrze, Poland.
Jerzy
J
Silberring
Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, 30-059, Krakow, Poland.|Academic Centre for Materials and Nanotechnology (ACMiN), AGH University of Science and Technology, 30-059, Krakow, Poland.
Piotr
P
Suder
D000368:Aged; D001807:Blood Vessel Prosthesis; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization
D008055:Lipids
cholesterol plaque; molecular imaging; vascular graft