[Identification of major components of Xingsusan decoction by HPLC-HR-TOF/MS].
OBJECTIVE
The major components of Xingsusan decoction were studied by HPLC-HR-TOF-MS.


METHODS
A Welchrom- C18 column (4.6 mm x 250 mm, 5 μm) was used at a flow rate of 1.0 mL/min. The HPLC gradient was acetonitrile-formic acid (99.9: 0.1, V/V) (B) and water-formic acid (99.9:0.1, V/V) (A). HR-MS/MS analysis were carried out using a Micro/TOF-Q II Focus mass spectrometer fitted with an ESI source operating in Auto-MSn mode to obtain fragment ions. TIC chemical profiles of the extract were established in both positive and negative modes. The structures of the known components in Xingsusan decoction were identified by comparing their retention times, molecular weight and CID fragmentation patterns with their corresponding compounds reported in the literature.


RESULTS
A total of 52 components were simultaneously detected in Q-TOF/MS.


CONCLUSIONS
This method can be used as reference for other Chinese Medicine formulas study.
Jian-Zhong
JZ
Ding
Cheng-Wu
CW
Song
Sheng
S
Zhang
Jun-Yi
JY
Liu
Rong-Zeng
RZ
Huang
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005561:Formates; D053719:Tandem Mass Spectrometry
D000097:Acetonitriles; D004365:Drugs, Chinese Herbal; D005561:Formates; C030544:formic acid; C032159:acetonitrile