IGF1 regulation of BOULE and CDC25A transcripts via a testosterone-independent pathway in spermatogenesis of adult mice.
The Deleted in AZoospermia (DAZ) gene family plays an essential role in spermatogenesis and fertility in mammals. This gene family contains two autosomal genes, BOULE and DAZL (DAZ-Like), and the DAZ gene cluster in the Y chromosome. CDC25A (a cell cycle regulator) has been proposed as a putative substrate for the RNA-binding proteins of DAZ family. However, mechanisms regulating DAZ gene expression have been poorly investigated. We analyzed immunohistochemical localization of DAZL, BOULE and CDC25A, as well as the involvement of testosterone (T) and insulin-like growth factor 1 (IGF1) in the modulation of mRNA expression for DAZL, BOULE and CDC25A in the adult mouse testes. It was found that DAZL was mostly immunolocalized in spermatogonia, while BOULE and CDC25A were detected in spermatocytes and round spermatids. Three-color immunofluorescence showed that DAZL-positive cells also expressed proliferating cell nuclear antigen (PCNA). In vitro incubation of the testes showed that neither T nor IGF1 affected DAZL mRNA expression. However, either T or IGF1 increased BOULE mRNA expression. Antiandrogen flutamide abolished the T-induced increase in BOULE mRNA, but had no effect on the IGF1 induced increase in the mouse testes. Extracellular-signal-regulated kinase 1/2 (ERK1/2) inhibitor, U0126, prevented IGF1-induction of BOULE mRNA. It was found that IGF1 increased CDC25A mRNA expression and that U0126 - but not flutamide - abolished the IGF1-induced CDC25A mRNA expression. These results showed that IGF1 regulated the expression of BOULE and CDC25A mRNAs via ERK1/2 signaling and in T-independent pathway during spermatogenesis in the adult mouse testes.
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD, Universidad Maimónides, Hidalgo 775, C1405BCK, Buenos Aires, Argentina(1). Electronic address: gonzalez.candela@maimonides.edu.
Candela R
CR
Gonzalez
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD, Universidad Maimónides, Hidalgo 775, C1405BCK, Buenos Aires, Argentina(1).
Verónica B
VB
Dorfman
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD, Universidad Maimónides, Hidalgo 775, C1405BCK, Buenos Aires, Argentina(1).
Alfredo D
AD
Vitullo
D000818:Animals; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005485:Flutamide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*; D046509:Tissue Culture Techniques; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism*
C551589:Boll protein, mouse; C508515:DAZL protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C519530:insulin-like growth factor-1, mouse; D013739:Testosterone; D012313:RNA; D007334:Insulin-Like Growth Factor I; D005485:Flutamide; D048049:Extracellular Signal-Regulated MAP Kinases; C517005:Cdc25a protein, mouse; D020687:cdc25 Phosphatases
BOULE; CDC25A; IGF1; Spermatogenesis; Testosterone