Expression pattern of RAGE and IGF-1 in the human fetal ovary and ovarian serous carcinoma.
The expression pattern of RAGE and IGF-1 proteins in different ovarian cell lineages was histologically analyzed in six fetal, nine adult human ovaries, and nine serous ovarian carcinomas (OSC) using immunohistochemical methods. Mild expression of IGF-1 in ovarian surface epithelium (Ose) and oocytes in the 15-week human ovaries increased to moderate or strong in the stromal cells, oocytes and follicular cells in week 22. Occasional mild RAGE expression was observed in Ose during week 15, while strong expression characterized primordial follicles in week 22. In the reproductive human ovary, IGF-1 was mildly to moderately expressed in all ovarian cell lineages except in theca cells of the tertiary follicle where IGF-1 was negative. RAGE was strongly positive in the granulosa cells and some theca cells of the tertiary follicle, while negative to mildly positive in all cells of the secondary follicle. In the postmenopausal human ovary IGF-1 and RAGE were mildly expressed in Ose and stroma. In OSC, cells were strongly positive to IGF-1 and RAGE, except for some negative stromal cells. Different levels of IGF-1 and RAGE co-expression characterized fetal ovarian cells during development. In reproductive ovaries, IGF-1 and RAGE were co-localized in the granulosa and theca interna cells of tertiary follicles, while in postmenopausal ovaries and OSC, IGF-1 and RAGE were co-localized in Ose and OSC cells respectively. Our results indicate that intracellular levels of IGF-1 and RAGE protein might regulate the final destiny of the ovarian cell populations prior and during folliculogenesis, possibly controlling the metastatic potential of OSC as well.
Laboratory for Early Human Development, Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia.
Ana
A
Poljicanin
Laboratory for Early Human Development, Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia.
Natalija
N
Filipovic
Department of Gynecology, University Hospital in Split, Spinciceva 1, 21000 Split, Croatia.
Tanja
T
Vukusic Pusic
Department of Pathology, Cytology and Forensic Medicine, University Hospital in Mostar, Kralja Tvrtka bb, 88 000 Mostar, Bosnia and Herzegovina.
Violeta
V
Soljic
Laboratory for Early Human Development, Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia.
Ana
A
Caric
Laboratory for Early Human Development, Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia.
Mirna
M
Saraga-Babic
Laboratory for Early Human Development, Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia. Electronic address: katarina.vukojevic@mefst.hr.
Katarina
K
Vukojevic
D000328:Adult; D005260:Female; D005333:Fetus / Q000196:embryology* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009865:Oocytes / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000196:embryology* / Q000473:pathology; D000067759:Receptor for Advanced Glycation End Products / Q000096:biosynthesis*
C000593871:AGER protein, human; C583557:IGF1 protein, human; D009363:Neoplasm Proteins; D000067759:Receptor for Advanced Glycation End Products; D007334:Insulin-Like Growth Factor I
Human fetus; IGF-1; Ovary; RAGE; Serous ovarian carcinoma