The prognostic significance of Src and p-Src expression in patients with osteosarcoma.
BACKGROUND
The expression of Src and phospho-Src (p-Src) is closely related to tumor invasion and metastasis. The aim of the present study was to investigate the expression of these molecules in osteosarcoma and their relationship with each other, to provide a theoretical basis to understand the prognosis of osteosarcoma.


METHODS
We selected surgically resected osteosarcoma specimens from 116 patients of Zhongnan Hospital of Wuhan University and Hubei Cancer Hospital, Hubei, China, between January 2000 and January 2010 with detailed follow-up data. Twenty osteochondroma specimens from the corresponding period were used as controls. Expression of Src and p-Src was detected in osteosarcoma and osteochondroma by immunohistochemistry. We analyzed the relationship of the 2 proteins and osteosarcoma patient prognosis.


RESULTS
The expression of Src and p-Src in osteosarcoma was significantly higher than the expression level in osteochondroma (P<0.05). The expression levels of the 2 proteins, clinical stage, and tumor metastasis were significantly associated with survival time (P<0.05), but there was no correlation between age or sex and survival time. The expression of Src and p-Src in osteosarcoma was positively correlated.


CONCLUSIONS
Src and p-Src can be used as an auxiliary indicator to determine a malignant phenotype of bone tumors, and the combined detection of Src and p-Src may indicate the prognosis of osteosarcoma.
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Chao
C
Hu
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Zhouming
Z
Deng
Department of Orthopedics, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China (mainland).
Yi
Y
Zhang
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Lei
L
Yan
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Lin
L
Cai
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Jun
J
Lei
Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China (mainland).
Yuanlong
Y
Xie
D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D015831:Osteochondroma / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D010766:Phosphorylation; D011182:Postoperative Care; D011379:Prognosis; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D055815:Young Adult
D016392:Proto-Oncogene Proteins pp60(c-src)