SNIPER peptide-mediated degradation of endogenous proteins.
Rapid and reversible methods for altering the function of endogenous proteins are not only indispensable tools for probing complex biological systems, but may potentially drive the development of new therapeutics for the treatment of human diseases. Genetic approaches have provided insights into protein function, but are limited in speed, reversibility and spatiotemporal control. To overcome these limitations, we have developed a peptide-based method (SNIPER: Selective Native Protein Eradication) to degrade any given endogenous protein at the post-translational level by harnessing chaperone-mediated autophagy, a major intracellular protein degradation pathway. This unit presents a typical strategy in the design and validation of a protein-knockdown peptide.
Brain Research Centre and Department of Medicine, Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia, Canada.
Xuelai
X
Fan
Yu Tian
YT
Wang
D000818:Animals; D001343:Autophagy; D005821:Genetic Techniques; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D011485:Protein Binding; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D059748:Proteolysis
D010455:Peptides; D011506:Proteins
chaperone-mediated autophagy; lysosome; peptide; protein knockdown