Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene.
ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Northeast Region, Ministry of Agriculture, The Northeast Agriculture University, Harbin, China.
J X
JX
Juan
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Northeast Region, Ministry of Agriculture, The Northeast Agriculture University, Harbin, China yxh100@sohu.com.
X H
XH
Yu
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Northeast Region, Ministry of Agriculture, The Northeast Agriculture University, Harbin, China.
X M
XM
Jiang
Harbin Baidu Science and Technology Development Co., Ltd., Northeast Agricultural University, Harbin, Heilongjiang, China.
Z
Z
Gao
Heilongjiang Academy of Sciences Natural and Ecological Research Institute, Xiangfang District, Harbin, Heilongjiang, China.
Y
Y
Zhang
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Northeast Region, Ministry of Agriculture, The Northeast Agriculture University, Harbin, China.
W
W
Li
Berries Research Institute, Heilongjiang Academy of Agricultural Sciences, Suiling, Heilongjiang, China.
Y D
YD
Duan
Berries Research Institute, Heilongjiang Academy of Agricultural Sciences, Suiling, Heilongjiang, China.
G
G
Yang
D000222:Adaptation, Physiological / Q000187:drug effects; D060054:Agrobacterium / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002228:Carbenicillin / Q000494:pharmacology; D003080:Cold Temperature; D018548:Cotyledon / Q000187:drug effects / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D017343:Genes, Plant; D005822:Genetic Vectors / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects
D000900:Anti-Bacterial Agents; C510502:ICE1 protein, Arabidopsis; D014157:Transcription Factors; D007612:Kanamycin; D002228:Carbenicillin