Gene manipulation for regenerative studies using the Iberian ribbed newt, Pleurodeles waltl.
Newts provide a unique model animal for regenerative studies. An experimental model system for molecular genetics in newts is needed in order to clarify the mechanisms of regeneration from the perspective of gene functions. We have identified that Iberian ribbed newt (Pleurodeles waltl) is a suitable model animal for such studies. Here we describe protocols for gene manipulation using Pleurodeles waltl.
Department of Biomedical Sciences, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, 683-8503, Tottori, Japan, toshi2@med.tottori-u.ac.jp.
Toshinori
T
Hayashi
Takashi
T
Takeuchi
D000822:Animal Husbandry; D000818:Animals; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics; D007315:Insemination, Artificial; D008297:Male; D008845:Microinjections; D010957:Plasmids / Q000235:genetics; D010999:Pleurodeles / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000235:genetics*
D012333:RNA, Messenger; D015252:Deoxyribonucleases, Type II Site-Specific