Preparation and rebinding properties of protein-imprinted polysiloxane using mesoporous calcium silicate grafted non-woven polypropylene as matrix.
Calcium silicate particle containing mesoporous SiO2 (CaSiO3@SiO2) was grafted on the surface of non-woven polypropylene. The PP non-woven grafted calcium silicate containing mesoporous SiO2 (PP-g-CaSiO3@SiO2) was used as the matrix to prepare bovine serum albumin (BSA) molecularly imprinted polysiloxane (MIP) by using silanes as the functional monomers and BSA as the template. PP non-woven grafted BSA-imprinted polysiloxane (PP-g-CaSiO3@SiO2 MIP) was characterized by scanning electron microscope (SEM), Fourier transform infrared spectometry (FTIR) and drilling string compensator (DSC). Influence factors on the rebinding capacity of the MIP were investigated, such as grafting degree, the pH in treating CaSiO3 and the type and proportion of silanes. The rebinding properties of BSA on PP-g-CaSiO3@SiO2 and MIP were investigated under different conditions. The results indicated that the rebinding capacity of MIP for BSA reached 56.32 mg/g, which was 2.65 times of NIP. The non-woven polypropylene grafted BSA-imprinted polysiloxane could recognize the template protein and the selectivity factor (β) was above 2.4 when using ovalbumin, hemoglobin and γ-globulin as control proteins. The PP-g-CaSiO3@SiO2 MIP has favorable reusability.
The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Bohong
B
Kan
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin, China.
Lingzhi
L
Feng
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin, China.
Kongyin
K
Zhao
The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Junfu
J
Wei
Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, China.
Dunwan
D
Zhu
Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, China.
Linhua
L
Zhang
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin, China.
Qian
Q
Ren
D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D015394:Molecular Structure; D011126:Polypropylenes / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D017640:Silicates / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D017610:Calcium Compounds; D011126:Polypropylenes; D011506:Proteins; D017640:Silicates; D012833:Siloxanes; C031293:calcium silicate
graft; mesoporous calcium silicate; non-woven polypropylene; polysiloxane; protein molecular imprinting