Effects of sleeve gastrectomy and gastric banding on the hypothalamic feeding center in an obese rat model.
OBJECTIVE
Laparoscopic sleeve gastrectomy (SG) and gastric banding (GB) are popular bariatric procedures for treating morbid obesity. This study aimed to investigate changes in the hypothalamic feeding center after these surgeries in a diet-induced obese rat model.


METHODS
Obesity was induced in 60 Sprague-Dawley rats using a high-energy diet for 6 weeks. These rats were divided into four groups: the sham-operated (SO) control, pair-fed (PF) control, SG and GB groups. Six weeks after the surgery, metabolic parameters, the plasma levels of leptin, ghrelin, peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) and the hypothalamic mRNA expressions of neuropeptide Y (NPY) and pro-opiomelanocortin (POMC) were measured.


RESULTS
Compared with those observed in the SO group, the body and fat tissue weights were significantly decreased and the metabolic parameters were significantly improved in the PF, SG and GB groups 6 weeks after surgery. The plasma ghrelin levels were significantly lower and the PYY and GLP-1 levels were significantly higher in the SG group than in the PF, GB and SO groups. Compared with that seen in the PF and GB groups, the hypothalamic mRNA expression of NPY was significantly lower and the expression of POMC was significantly higher in the SG group.


CONCLUSIONS
SG may affect the neurological pathway associated with appetite in the hypothalamus and thereby control ingestive behavior.
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan. kawasaki@oita-u.ac.jp.
Takahide
T
Kawasaki
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Masayuki
M
Ohta
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Yuichiro
Y
Kawano
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Takashi
T
Masuda
Department of Endocrinology, Metabolism, and Rheumatology, Oita University Faculty of Medicine, Oita, Japan.
Koro
K
Gotoh
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Masafumi
M
Inomata
Oita University, Oita, Japan.
Seigo
S
Kitano
D000818:Animals; D050110:Bariatric Surgery / Q000379:methods*; D004195:Disease Models, Animal; D005247:Feeding Behavior / Q000502:physiology*; D005743:Gastrectomy / Q000379:methods*; D054439:Ghrelin / Q000097:blood; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D007031:Hypothalamus / Q000378:metabolism / Q000503:physiopathology*; D020738:Leptin / Q000097:blood; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D009767:Obesity, Morbid / Q000378:metabolism / Q000503:physiopathology* / Q000523:psychology / Q000601:surgery*; D019894:Peptide YY / Q000097:blood; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley
D054439:Ghrelin; D020738:Leptin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D019894:Peptide YY; D011333:Pro-Opiomelanocortin; D052216:Glucagon-Like Peptide 1
Bariatric surgery; Gastric banding; Hypothalamus; Ingestive behavior; Sleeve gastrectomy