Genetic analysis of seed-shattering genes in rice using an F₃:₄ population derived from an Oryza sativa x Oryza rufipogon cross.
Seed shattering of wild plant species is thought to be an adaptive trait to facilitate seed dispersal. For rice breeding, seed shatter-ing is an important trait for improving breeding strategies, particularly when developing lines use interspecific hybrids and introgression of genes from wild species. We developed F₃:₄ recombinant inbred lines from an interspecific cross between Oryza sativa cv. Ilpoombyeo and Oryza rufipogon. In this study, we genetically analyzed known shat-tering-related loci using the F₃:₄ population of O. sativa/O. rufipogon. CACTA-AG190 was significantly associated with the shattering trait CACTA-TD according to bulked segregant analysis results, and was found in the qSH-1 region of chromosome 1. Fine genetic mapping of the flanking regions around qSH-1 based on CACTA-AG190 revealed multiple-sequence variations. The highest limit of detection based on quantitative trait locus analysis was observed between shaap-7715 and a 518-bp insertion site. Two other quantitative trait locus analyses of seed-shattering-related loci, qSH-4 and sh-h, were performed using simple sequence repeat and allele-pecific single nucleotide polymor-phism markers. Our results can be applied for rice-breeding research, such as marker-assisted selection between cultivated and wild rice.
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
S-J
SJ
Kwon
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
J
J
Yu
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
Y J
YJ
Park
Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
J-H
JH
Son
Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
N-S
NS
Kim
Department of Applied Plant Sciences, Oriental Bio-Herb Research Institute, Kangwon National University, Chuncheon, Korea jukyonglee@kangwon.ac.kr.
J K
JK
Lee
D000483:Alleles; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D005820:Genetic Testing; D018895:Microsatellite Repeats; D008957:Models, Genetic; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D058614:Seed Dispersal / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity
D004251:DNA Transposable Elements; D018744:DNA, Plant; D005819:Genetic Markers