Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB.
The study investigated the role of wnt1 in the inflammatory response initiated by lipolysaccharide (LPS), and analyzed the association between wnt1, NF-KB, and inflammatory factors. THP-1 cells were activated with phorbol-12-myristate-13-acetate (PMA) and treated with LPS to induce inflammation. THP-1 cells were transfected with wnt1siRNA and overexpression plasmid to explore the relationship among wnt1, SRA, and NF-KB. Inhibitor of β-catenin and siRNA of FZD1were used to investigate the signaling events involved in SRA activation induced by wnt1. Levels of NF-kB protein and inflammatory cytokines were assessed followingwnt1 siRNA and LPS treatment. PMA activation and LPS treatment of THP-1 cells increased wnt1 protein levels. Wnt1 promoted SRA expression through activation of canonical wnt pathway. Wnt1 increased NF-kB protein levels and enhanced the secretion of IL-6, TNF-α, and iNOS through binding to SRA. These findings suggest that wnt1 increased SRA and NF-kB protein levels and participated in the inflammatory response.
Department of Cardiology, First Affiliated Hospital, College of Medicine, Zhejiang University, 79Qingchun Road, Hangzhou, 310003, China.
Wenting
W
Zhao
Zewei
Z
Sun
Shuai
S
Wang
Zhenwei
Z
Li
Liangrong
L
Zheng
D002460:Cell Line; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis*; D051117:Scavenger Receptors, Class A / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*; D051155:Wnt1 Protein / Q000096:biosynthesis* / Q000494:pharmacology
D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051117:Scavenger Receptors, Class A; C495247:WNT1 protein, human; D051155:Wnt1 Protein