A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on microfluidic compact disk platform.
In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10(-3) ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.
Shah Mukim
SM
Uddin
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. fatimah@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. fatimah@um.edu.my.
Fatimah
F
Ibrahim
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.
Abkar Ahmed
AA
Sayad
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.
Aung
A
Thiha
Microbiology Unit, Institute of Biological Sciences, Faculty of Science, University of Malaya 50603 Kuala Lumpur, Malaysia. xp_527@yahoo.com.
Koh Xiu
KX
Pei
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.
Mas S
MS
Mohktar
Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. uda@unimap.edu.my.
Uda
U
Hashim
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.|Department of Biomedical Engineering, Inje University, Gimhae 621-749, South Korea. thongkl@um.edu.my.
Jongman
J
Cho
Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. xp_527@yahoo.com.|Microbiology Unit, Institute of Biological Sciences, Faculty of Science, University of Malaya 50603 Kuala Lumpur, Malaysia. xp_527@yahoo.com.
Kwai Lin
KL
Thong
D016250:Compact Disks; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D005516:Food Microbiology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification* / Q000472:pathogenicity
D004269:DNA, Bacterial