Protective effects of Sesamum indicum extract against oxidative stress induced by vanadium on isolated rat hepatocytes.
Vanadium toxicity is a challenging problem to human and animal health with no entirely understanding cytotoxic mechanisms. Previous studies in vanadium toxicity showed involvement of oxidative stress in isolated liver hepatocytes and mitochondria via increasing of ROS formation, release of cytochrome c and ATP depletion after incubation with different concentrations (25-200 µM). Therefore, we aimed to investigate the protective effects of Sesamum indicum seed extract (100-300 μg/mL) against oxidative stress induced by vanadium on isolated rat hepatocytes. Our results showed that quite similar to Alpha-tocopherol (100 µM), different concentrations of extract (100-300 μg/mL) protected the isolated hepatocyte against all oxidative stress/cytotoxicity markers induced by vanadium in including cell lysis, ROS generation, mitochondrial membrane potential decrease and lysosomal membrane damage. Besides, vanadium induced mitochondrial/lysosomal toxic interaction and vanadium reductive activation mediated by glutathione in vanadium toxicity was significantly (P < 0.05) ameliorated by Sesamum indicum extracts. These findings suggested a hepato-protective role for extracts against liver injury resulted from vanadium toxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 979-985, 2016.
Department of Toxicology and Pharmacology, Zanjan Applied Pharmacology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Mir-Jamal
MJ
Hosseini
Faculty of Pharmacy, Department of Pharmacology and Toxicology, Zabol University of Medical Sciences, Sistan and Baluchestan, Iran.
Jafar
J
Shahraki
Faculty of Pharmacy, Department of Pharmacology and Toxicology, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran.
Saman
S
Tafreshian
Faculty of Pharmacy, Department of Pharmacology and Toxicology, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran.
Ahmad
A
Salimi
Faculty of Pharmacy, Department of Pharmacology and Toxicology, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran.
Mohammad
M
Kamalinejad
Faculty of Pharmacy, Department of Pharmacology and Toxicology, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran.
Jalal
J
Pourahmad
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D031685:Sesamum / Q000737:chemistry; D014639:Vanadium / Q000633:toxicity*
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014639:Vanadium; D045304:Cytochromes c; D005978:Glutathione
hepato-protection; hepatocytes; oxidative stress; sesamum indicum; vanadium