Constitutive arsenite oxidase expression detected in arsenic-hypertolerant Pseudomonas xanthomarina S11.
Pseudomonas xanthomarina S11 is an arsenite-oxidizing bacterium isolated from an arsenic-contaminated former gold mine in Salsigne, France. This bacterium showed high resistance to arsenite and was able to oxidize arsenite to arsenate at concentrations up to 42.72 mM As[III]. The genome of this strain was sequenced and revealed the presence of three ars clusters. One of them is located on a plasmid and is organized as an "arsenic island" harbouring an aio operon and genes involved in phosphorous metabolism, in addition to the ars genes. Neither the aioXRS genes nor a specific sigma-54-dependent promoter located upstream of aioBA genes, both involved in regulation of arsenite oxidase expression in other arsenite-oxidizing bacteria, could be identified in the genome. This observation is in accordance with the fact that no difference was observed in expression of arsenite oxidase in P. xanthomarina S11, whether or not the strain was grown in the presence of As[III].
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: sandrine.koechler@unistra.fr.
Sandrine
S
Koechler
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: ploetze@unistra.fr.
Florence
F
Arsène-Ploetze
Université de Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, 43 boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France. Electronic address: celine.brochier-armanet@univ-lyon1.fr.
Céline
C
Brochier-Armanet
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: florencegoulhen@yahoo.com.
Florence
F
Goulhen-Chollet
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: salmeron_audrey@yahoo.fr.
Audrey
A
Heinrich-Salmeron
Plateforme Biopuces et séquençage, IGBMC, 1 rue Laurent Fries Parc d'Innovation, 67400 Illkirch, France. Electronic address: jost@igbmc.fr.
Bernard
B
Jost
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: didier.lievremont@unistra.fr.
Didier
D
Lièvremont
Plateforme Biopuces et séquençage, IGBMC, 1 rue Laurent Fries Parc d'Innovation, 67400 Illkirch, France. Electronic address: mkoch@igbmc.fr.
Muriel
M
Philipps
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: f.plewniak@unistra.fr.
Frédéric
F
Plewniak
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: philippe.bertin@unistra.fr.
Philippe N
PN
Bertin
UMR7156 Université de Strasbourg/CNRS Génétique Moléculaire, Génomique, Microbiologie, Département Micro-organismes, Génomes, Environnement, 28 rue Goethe, 67083 Strasbourg Cedex, France. Electronic address: lett@unistra.fr.
Marie-Claire
MC
Lett
D001149:Arsenates / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism* / Q000494:pharmacology; D053501:Arsenite Transporting ATPases / Q000235:genetics / Q000378:metabolism; D018053:Arsenites / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial; D005602:France; D015964:Gene Expression Regulation, Bacterial; D008906:Mining; D009876:Operon; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification
D001149:Arsenates; D018053:Arsenites; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010088:Oxidoreductases; C078179:arsenite oxidase; D053501:Arsenite Transporting ATPases; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid
Arsenic tolerance; Genome; Metal; Resistance