The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites. In resistant host plants, relatively few females develop to maturity. Little is known about the physiological and biochemical changes induced during CCN infection. Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis. Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation. Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan. Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars. In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition. During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections. Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Jessika
J
Aditya
South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia.
John
J
Lewis
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Neil J
NJ
Shirley
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Hwei-Ting
HT
Tan
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Marilyn
M
Henderson
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Geoffrey B
GB
Fincher
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Rachel A
RA
Burton
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Diane E
DE
Mather
Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia.
Matthew R
http://orcid.org/0000-0003-4661-6700
MR
Tucker
D000818:Animals; D002473:Cell Wall; D016000:Cluster Analysis; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D004198:Disease Susceptibility; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015726:Giant Cells / Q000502:physiology; D060434:Herbivory / Q000502:physiology; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000469:parasitology*; D009005:Monosaccharides / Q000032:analysis; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology; D011134:Polysaccharides / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014431:Tylenchoidea / Q000502:physiology*; D047071:beta-Glucans / Q000378:metabolism*
D009005:Monosaccharides; D010940:Plant Proteins; D011134:Polysaccharides; D012333:RNA, Messenger; D047071:beta-Glucans
Heterodera avenae; Hordeum vulgare; barley; cell wall; cellulose synthase-like; cereal cyst nematode (CCN)