Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells.
Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
Xiyun
X
Ruan
Shandong Medical Imaging Research Institute, Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
Zhenguo
Z
Yuan
Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
Yifeng
Y
Du
Xi'an Huaguang Biological Engineering Co., Ltd., Xi'an 710025, Shaanxi Province, China.
Guangxiao
G
Yang
Xi'an Huaguang Biological Engineering Co., Ltd., Xi'an 710025, Shaanxi Province, China.
Quanying
Q
Wang
antimicrobial peptide PR39; apoptosis; fusion gene; gene therapy; human thioredoxin; hypoxia; recombinant adeno-associated virus