Metagenome of a microbial community inhabiting a metal-rich tropical stream sediment.
Here, we describe the metagenome and functional composition of a microbial community in a historically metal-contaminated tropical freshwater stream sediment. The sediment was collected from the Mina Stream located in the Iron Quadrangle (Brazil), one of the world's largest mining regions. Environmental DNA was extracted and was sequenced using SOLiD technology, and a total of 7.9 Gbp was produced. A taxonomic profile that was obtained by comparison to the Greengenes database revealed a complex microbial community with a dominance of Proteobacteria and Parvarcheota. Contigs were recruited by bacterial and archaeal genomes, especially Candidatus Nitrospira defluvii and Nitrosopumilus maritimus, and their presence implicated them in the process of N cycling in the Mina Stream sediment (MSS). Functional reconstruction revealed a large, diverse set of genes for ammonium assimilation and ammonification. These processes have been implicated in the maintenance of the N cycle and the health of the sediment. SEED subsystems functional annotation unveiled a high degree of diversity of metal resistance genes, suggesting that the prokaryotic community is adapted to metal contamination. Furthermore, a high metabolic diversity was detected in the MSS, suggesting that the historical arsenic contamination is no longer affecting the prokaryotic community. These results expand the current knowledge of the microbial taxonomic and functional composition of tropical metal-contaminated freshwater sediments.
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Patrícia S
PS
Costa
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Mariana P
MP
Reis
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Marcelo P
MP
Ávila
Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
Laura R
LR
Leite
Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
Flávio M G
FM
de Araújo
Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
Anna C M
AC
Salim
Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
Guilherme
G
Oliveira
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Francisco
F
Barbosa
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Edmar
E
Chartone-Souza
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Andréa M A
AM
Nascimento
D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001938:Brazil; D004783:Environmental Microbiology; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D054892:Metagenome; D008670:Metals / Q000378:metabolism; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017423:Sequence Analysis, RNA; D014329:Tropical Climate
D008670:Metals