The minimum peptides of IGF-1 and substance P protect vestibular hair cells against neomycin ototoxicity.
CONCLUSIONS
Our data indicate that SSSR and SSSR + FGLM-NH2 protect sensory hair cells against neomycin-induced death in the vestibular epithelium. In addition, the results show that SSSR and FGLM-NH2 can be used as protective molecules against aminoglycoside ototoxicity.


OBJECTIVE
This study investigated the role of the peptides SSSR and SSSR + FGLM-NH2 in mammalian vestibular hair cell death induced by aminoglycoside.


METHODS
Cultured utricles from mature CBA/N mice were used in this study. The cultured utricles were assigned to five groups (control group, neomycin group, neomycin + SSSR group, neomycin + FGLM-NH2 group, and neomycin + SSSR + FGLM-NH2 group). Aat 24 h after exposure to neomycin, the hair cells were labeled immunohistochemically, and the rate of survival of vestibular hair cells was evaluated using a fluorescence microscope.


RESULTS
The rate of survival of vestibular hair cells was significantly higher in the neomycin + SSSR and neomycin + SSSR + FGLM-NH2 groups than in the neomycin group. The results suggest that SSSR could protect hair cells against aminoglycoside ototoxicity.
Department of Otolaryngology, Yamaguchi University Graduate School of Medicine , Ube, Yamaguchi , Japan.
Shuhei
S
Yoshida
Kazuma
K
Sugahara
Makoto
M
Hashimoto
Yoshinobu
Y
Hirose
Hiroaki
H
Shimogori
Hiroshi
H
Yamashita
D000818:Animals; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D004357:Drug Synergism; D018069:Hair Cells, Vestibular / Q000187:drug effects* / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008808:Mice, Inbred CBA; D008856:Microscopy, Fluorescence; D009355:Neomycin / Q000633:toxicity*; D009842:Oligopeptides / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology*
D009842:Oligopeptides; C519530:insulin-like growth factor-1, mouse; C112946:phenylalanyl-glycyl-leucyl-methioninamide; C530429:seryl-seryl-seryl-arginine; D013373:Substance P; D007334:Insulin-Like Growth Factor I; D009355:Neomycin
FGLM-NH2; SSSR; aminoglycoside; hair cell degeneration