A mutation in the human tetraspanin CD81 gene is expressed as a truncated protein but does not enable CD19 maturation and cell surface expression.
A homozygous mutation in a splice site of the CD81 gene was identified previously in a patient, as the cause in a case of common variable immune deficiency (CVID). CD19 expression is reduced in mice that lack CD81; however, B cells in this patient lacked completely CD19 surface expression. The mutation led to an absence of the CD81 protein on the cell surface and it was assumed that the CD81 protein was not produced. Here we demonstrate that a truncated human CD81 mutant (CD81mut) was actually produced, but retained intracellularly. We also demonstrate that the truncated CD81mut protein is in close proximity to the intracellularly sequestered CD19. However, this interaction does not enable normal CD19 maturation and surface expression. In addition, we show that specific domains of CD81 enable retrieval and trafficking of human CD19 to the cell surface. Finally, we demonstrate that surface expression of CD19 requires CD81, even in non-B cells.
Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, CA, USA.
Felipe
F
Vences-Catalán
Chiung-Chi
CC
Kuo
Yael
Y
Sagi
Homer
H
Chen
Neta
N
Kela-Madar
Menno C
MC
van Zelm
Jacques J M
JJ
van Dongen
Shoshana
S
Levy
D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D051379:Mice; D009154:Mutation; D021381:Protein Transport; D060225:Tetraspanin 28 / Q000235:genetics / Q000378:metabolism*
D018941:Antigens, CD19; C554798:CD81 protein, human; D060225:Tetraspanin 28