[Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro].
OBJECTIVE
To investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes in vitro.


METHODS
In vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or without pretreatment with PP2 (10 µmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells were determined using Western blotting.


RESULTS
PP2 pretreatment significantly increased the viability and decreased the apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R injury, and was significantly decreased by PP2 pretreatment (P<0.01).


CONCLUSIONS
PP2 protects rat astrocytes from H/R injury possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.
Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China.E-mail: guyuchen07@163.com.
Yuchen
Y
Gu
Xuhui
X
Tong
Li
L
Yu
Hao
H
Jiao
Binbin
B
Yu
Shuying
S
Dong
D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000473:pathology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005434:Flow Cytometry; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D019061:src-Family Kinases / Q000037:antagonists & inhibitors*
C412373:AG 1879; D011743:Pyrimidines; D019061:src-Family Kinases