Regulatory function of β1,4-galactosyltransferase I expression on Lewis-Y glycan and embryo implantation.
β1,4-Galactosyltransferase I (β1,4-GalT-I), a key enzyme in glycobiology, mediates several biological mechanisms. However, the correlation between β-1,4-GalT-I expression in the uterine endometrium and embryo implantation remains unclear. This study aims to elucidate the relationship between β1,4-GalT-I and Lewis(Y) (Le(Y)) glycan during embryo implantation. So far, using green fluorescent protein as an indicator, β1,4-GalT-I interference plasmid (pcDNA3.0-siGalT I), overexpression plasmid (pcDNA3.0-HA-GalT I), interference control plasmid (control pcDNA3.0-siGalT I), and empty vector (pcDNA3.0) were transfected into human uterine epithelial RL95-2 cells that imitate the receptive endometrium. Invasive embryos at pre-implantation and treated RL95-2 cells were co-cultured to determine embryo attachment in each of the transfection groups. The results showed that plasmid transfection was successful in all the groups. β1,4-GalT-I and Fucosyltransferase 1 (FUT1) gene expression declined in the interference group, and the synthesis of Le(Y) decreased accordingly, but the expression of this antigen increased in the overexpression group. After co-culturing of the embryos and 36h transfection of RL95-2, the results of these in vitro implantation models showed that the attachment rate was lower in the interference group (30.0 ± 0.2%) than in the untreated group (50.0 ± 0.6%), empty vector group (50.0 ± 0.2%), and interference control group (46.7 ± 0.6%), however, it was highest in the overexpression group (70.0 ± 0.2%). These results indicated that β1,4-galactosyltransferase I possibly regulate mutual uterus-embryo adhesion and embryo implantation by regulating cell surface Le(Y) glycan expression.
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China; Reproduction Medicine Center, Xuzhou Central Hospital, Xuzhou 221009, China.
Juan
J
Gu
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China.
Jianhui
J
Fan
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China.
Yuefei
Y
Xu
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China.
Yunpeng
Y
Xie
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China.
Ting
T
Gong
Department of Biochemistry and Molecular Biology, Liaoning Provincial Key Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian 116044, China. Electronic address: kongying@dlmedu.edu.cn.
Ying
Y
Kong
D000818:Animals; D018920:Coculture Techniques; D046149:Embryo Culture Techniques; D010064:Embryo Implantation; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000378:metabolism*; D008297:Male; D051379:Mice; D009237:N-Acetyllactosamine Synthase / Q000235:genetics / Q000378:metabolism*; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D005647:Fucosyltransferases; D007983:Lewis Blood Group Antigens; D009237:N-Acetyllactosamine Synthase; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C094162:Lewis Y antigen
Embryo implantation; FUT1 gene; Le(Y) glycan; β1,4-Galactosyltransferase I