Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats.
Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Fengguo
F
Zhai
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Xiuping
X
Zhang
Department of Endocrinology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Yue
Y
Guan
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Xudong
X
Yang
Department of Thoracic Surgery, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Yang
Y
Li
Department of Biochemistry, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Gaochen
G
Song
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China.
Lixin
L
Guan
apoptosis; bcl-2; caspase-3; focal cerebral ischemia; microRNA; neural regeneration