Combining detection of Notch1 and tumor necrosis factor-α converting enzyme is a reliable biomarker for the diagnosis of abdominal aortic aneurysms.
OBJECTIVE
Although many markers were associated with abdominal aortic aneurysm (AAA), there is no clear consensus on which marker is of the most value. Studies have implicated the role of Notch signaling in the pathogenesis of AAA. We investigate the value of plasma Jagged1, Notch receptors and tumor necrosis factor-α converting enzyme (TACE) in identifying AAA.


METHODS
42 patients with AAA and 36 controls were enrolled in our study. The concentrations of plasma Jagged1, Notch receptors and TACE were measured by enzyme-linked immunosorbent assay (ELISA). The diagnostic value of plasma Notch1 and TACE was assessed by logistic regression and receiver operator characteristic (ROC) curve. Double immunofluorescence staining was used to investigate the distribution of Notch1 and TACE in AAA tissue specimens.


RESULTS
The concentrations of plasma Notch1 and TACE were significantly higher in AAA than in the controls, respectively (Notch1: P < 0.001; TACE: P = 0.0001). The area under the curve (AUC) from ROC curve of plasma Notch1 and TACE in determining the presence of AAA was 0.878 and 0.804, respectively. Combining detection of plasma Notch1 and TACE could improve the accuracy in detecting AAA (AUC 0.984, P < 0.0001). The predicted probability cutoff of 0.70 gave a sensitivity of 90.5% and a specificity of 100% for combining detection of plasma Notch1 and TACE in predicting AAA.


CONCLUSIONS
This is the first report revealing that plasma Notch1 and TACE are highly expressed in AAA. Combining detection of plasma Notch1 and TACE may be reliable for identifying the presence of AAA.
Department of Vascular Surgery, Peking Union Medical Hospital, Beijing, China; Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Yue-Wei
YW
Wang
Department of Vascular Surgery, Peking Union Medical Hospital, Beijing, China.
Hua-Liang
HL
Ren
Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address: wanghf2003@126.com.
Hao-Fu
HF
Wang
Department of Vascular Surgery, Peking Union Medical Hospital, Beijing, China.
Fang-Da
FD
Li
Department of Physiology and Physiopathology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. Electronic address: hhli935@aliyun.com.
Hui-Hua
HH
Li
Department of Vascular Surgery, Peking Union Medical Hospital, Beijing, China. Electronic address: yuehongzheng@yahoo.com.
Yue-Hong
YH
Zheng
D051722:ADAM Proteins / Q000032:analysis* / Q000097:blood; D000072198:ADAM17 Protein; D000368:Aged; D000369:Aged, 80 and over; D017544:Aortic Aneurysm, Abdominal / Q000097:blood / Q000175:diagnosis*; D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000097:blood; D000072100:Jagged-1 Protein; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000097:blood; D008875:Middle Aged; D051881:Receptor, Notch1 / Q000032:analysis* / Q000097:blood; D051880:Receptors, Notch / Q000032:analysis / Q000097:blood; D015203:Reproducibility of Results; D000072099:Serrate-Jagged Proteins
D015415:Biomarkers; D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
Abdominal aortic aneurysm; Notch signaling; Notch1; TACE