Halorubrum persicum sp. nov., an extremely halophilic archaeon isolated from sediment of a hypersaline lake.
An extremely halophilic archaeon belonging to the genus Halorubrum, strain C49T, was isolated from sediment of the hypersaline lake Aran-Bidgol in Iran. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain C49T was closely related to Halorubrum saccharovorum JCM 8865T (99.5 %) and other species of the genus Halorubrum. Studies based on multilocus sequence analysis revealed that strain C49T is placed among the species of Halorubrum; the strain constituted a defined branch in comparison with the type strains of species of Halorubrum, while the 16S rRNA gene sequence divergence could not define the status of the newly isolated strain. For optimum growth, strain C49T required 20 % (w/v) salts at pH 7.0 and 37 °C under aerobic conditions. Mg2+ was not required. The cells were pleomorphic rods, motile and stained Gram-variable. Colonies of the strain were pink. Hypotonic treatment with <12 % NaCl provoked cell lysis. The polar lipid pattern of strain C49T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester derived from both C20C20 and C20C25 archaeol, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 64.2 mol%. DNA-DNA hybridization studies and average nucleotide identity confirmed that strain C49T constitutes a distinct genospecies. Data obtained in this study show that strain C49T represents a novel species, for which the name Halorubrum persicum sp. nov. is proposed. The type strain is C49T ( = IBRC-M 10232T = JCM 30541T).
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Paulina
P
Corral
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Rafael R
RR
de la Haba
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Cristina
C
Sánchez-Porro
Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
Mohammad Ali
MA
Amoozegar
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
R Thane
RT
Papke
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Antonio
A
Ventosa
D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019766:Genes, Archaeal; D019015:Geologic Sediments / Q000382:microbiology*; D055352:Halorubrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA
D019641:DNA, Archaeal; D005227:Fatty Acids; C407124:PGP-Me; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S