AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip.
Nitrogen and phosphorus are among the most widely used fertilizers worldwide. Nitrate (NO3(-)) and phosphate (PO4(3-)) are also signalling molecules whose respective transduction pathways are being intensively studied. However, plants are continuously challenged with combined nutritional deficiencies, yet very little is known about how these signalling pathways are integrated. Here we report the identification of a highly NO3(-)-inducible NRT1.1-controlled GARP transcription factor, HRS1, document its genome-wide transcriptional targets, and validate its cis-regulatory elements. We demonstrate that this transcription factor and a close homologue repress the primary root growth in response to P deficiency conditions, but only when NO3(-) is present. This system defines a molecular logic gate integrating P and N signals. We propose that NO3(-) and P signalling converge via double transcriptional and post-transcriptional control of the same protein, HRS1.
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Anna
A
Medici
Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York 10003, USA.
Amy
A
Marshall-Colon
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Elsa
E
Ronzier
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Wojciech
W
Szponarski
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093-0116, USA.
Rongchen
R
Wang
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Alain
A
Gojon
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093-0116, USA.
Nigel M
NM
Crawford
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Sandrine
S
Ruffel
Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York 10003, USA.
Gloria M
GM
Coruzzi
Biochimie et Physiologie Moléculaire des Plantes, Institut Claude Grignon, UMR5004 CNRS/INRA/Supagro-M/UM2, Place Viala, F-34060 Montpellier cedex 2, France.
Gabriel
0000000336936735
G
Krouk
D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D015151:Immunoblotting; D016013:Likelihood Functions; D018519:Meristem / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009566:Nitrates / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; D017931:DNA Primers; C558731:HRS1 protein, Arabidopsis; D009566:Nitrates; D010710:Phosphates; D014157:Transcription Factors