Retinal angiography with real-time speckle variance optical coherence tomography.
This report describes a novel, non-invasive and label-free optical imaging technique, speckle variance optical coherence tomography (svOCT), for visualising blood flow within human retinal capillary networks. This imaging system uses a custom-built swept source OCT system operating at a line rate of 100 kHz. Real-time processing and visualisation is implemented on a consumer grade graphics processing unit. To investigate the quality of microvascular detail acquired with this device we compared images of human capillary networks acquired with svOCT and fluorescein angiography. We found that the density of capillary microvasculature acquired with this svOCT device was visibly greater than fluorescein angiography. We also found that this svOCT device had the capacity to generate en face images of distinct capillary networks that are morphologically comparable with previously published histological studies. Finally, we found that this svOCT device has the ability to non-invasively illustrate the common manifestations of diabetic retinopathy and retinal vascular occlusion. The results of this study suggest that graphics processing unit accelerated svOCT has the potential to non-invasively provide useful quantitative information about human retinal capillary networks. Therefore svOCT may have clinical and research applications for the management of retinal microvascular diseases, which are a major cause of visual morbidity worldwide.
School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
Jing
J
Xu
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Sherry
S
Han
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada Centre for Ophthalmology and Visual Science, Lions Eye Institute, The University of Western Australia, Perth, Western Australia, Australia The ARC Centre of Excellence in Vision Science, The University of Western Australia, Perth, Western Australia, Australia.
Chandrakumar
C
Balaratnasingam
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Zaid
Z
Mammo
School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
Kevin S K
KS
Wong
School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
Sieun
S
Lee
School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
Michelle
M
Cua
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Mei
M
Young
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Andrew
A
Kirker
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
David
D
Albiani
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Farzin
F
Forooghian
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Paul
P
Mackenzie
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
Andrew
A
Merkur
Centre for Ophthalmology and Visual Science, Lions Eye Institute, The University of Western Australia, Perth, Western Australia, Australia The ARC Centre of Excellence in Vision Science, The University of Western Australia, Perth, Western Australia, Australia.
Dao-Yi
DY
Yu
School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada.
Marinko V
MV
Sarunic
D003930:Diabetic Retinopathy / Q000175:diagnosis* / Q000503:physiopathology; D005451:Fluorescein Angiography / Q000379:methods*; D005654:Fundus Oculi; D006801:Humans; D012039:Regional Blood Flow; D015203:Reproducibility of Results; D015356:Retinal Artery Occlusion / Q000175:diagnosis* / Q000503:physiopathology; D012170:Retinal Vein Occlusion / Q000175:diagnosis* / Q000503:physiopathology; D012171:Retinal Vessels / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000379:methods*
Imaging; Retina