Method of isolated ex vivo lung perfusion in a rat model: lessons learned from developing a rat EVLP program.
The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. As this technology expands and improves, the ability to potentially evaluate and improve the quality of substandard lungs prior to transplant is a critical need. In order to more rigorously evaluate these approaches, a reproducible animal model needs to be established that would allow for testing of improved techniques and management of the donated lungs as well as to the lung-transplant recipient. In addition, an EVLP animal model of associated pathologies, e.g., ventilation induced lung injury (VILI), would provide a novel method to evaluate treatments for these pathologies. Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs. The goal is to provide the research community with key information and "pearls of wisdom"/techniques that arose from trial and error and are critical to establishing an EVLP system that is robust and reproducible.
Department of Biomedical Engineering, Ohio State University Wexner Medical Center; Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center.
Kevin
K
Nelson
Department of Biomedical Engineering, Ohio State University Wexner Medical Center; Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center.
Christopher
C
Bobba
The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center.
Emre
E
Eren
Division of Cardiac Surgery, Department of Surgery, Ohio State University Wexner Medical Center.
Tyler
T
Spata
Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center.
Malak
M
Tadres
Departments of Pediatrics and Internal Medicine, Ohio State University; Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs, Nationwide Children's Hospital.
Don
D
Hayes
The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center; Division of Transplantation, Department of Surgery, Ohio State University Wexner Medical Center.
Sylvester M
SM
Black
Department of Biomedical Engineering, Ohio State University Wexner Medical Center; Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center; The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center; samir.ghadiali@osumc.edu.
Samir
S
Ghadiali
Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center; The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center; Division of Cardiac Surgery, Department of Surgery, Ohio State University Wexner Medical Center; Bryan.Whitson@osumc.edu.
Bryan A
BA
Whitson
D000818:Animals; D005112:Extracorporeal Circulation / Q000295:instrumentation / Q000379:methods; D008168:Lung / Q000098:blood supply* / Q000601:surgery*; D016040:Lung Transplantation / Q000295:instrumentation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D010477:Perfusion / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley