Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation.
In this work the surface-enhanced Raman scattering (SERS) spectra of five genomic DNAs from non-cryopreserved control tomato plants (Lycopersicon esculentum Mill. cultivars Siriana, Darsirius, Kristin, Pontica and Capriciu) respectively, have been analyzed in the wavenumber range 400-1800 cm(-1). Structural changes induced in genomic DNAs upon cryopreservation were discussed in detail for four of the above mentioned tomato cultivars. The surface-enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. We have found, that DNA isolated from Siriana cultivar leaf tissues suffers the weakest structural changes upon cryogenic storage of tomato shoot apices. On the contrary, genomic DNA extracted from Pontica cultivar is the most responsive system to cryopreservation process. Particularly, both C2'-endo-anti and C3'-endo-anti conformations have been detected. As a general observation, the wavenumber range 1511-1652 cm(-1), being due to dA, dG and dT residues seems to be influenced by cryopreservation process. These changes could reflect unstacking of DNA bases. However, not significant structural changes of genomic DNAs from Siriana, Darsirius and Kristin have been found upon cryopreservation process of tomato cultivars. Based on this work, specific plant DNA-ligand interactions or accurate local structure of DNA in the proximity of a metallic surface, might be further investigated using surface-enhanced Raman spectroscopy.
National Institute for Research & Development of Isotopic and Molecular Technologies, Donat 67-103, 400293 Cluj-Napoca, Romania. Electronic address: cmuntean@itim-cj.ro.
Cristina M
CM
Muntean
Babeş-Bolyai University, Faculty of Physics, Kogalniceanu 1, 400084 Cluj-Napoca, Romania.
Nicolae
N
Leopold
National Institute for Research & Development of Isotopic and Molecular Technologies, Donat 67-103, 400293 Cluj-Napoca, Romania.
Carmen
C
Tripon
Institute of Biological Research, Branch of National Institute of Research and Development for Biological Sciences, Republicii 48, 400015 Cluj-Napoca, Romania.
Ana
A
Coste
Institute of Biological Research, Branch of National Institute of Research and Development for Biological Sciences, Republicii 48, 400015 Cluj-Napoca, Romania.
Adela
A
Halmagyi
D003102:Colloids; D015925:Cryopreservation; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D018745:Genome, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development*; D018515:Plant Leaves / Q000235:genetics; D013059:Spectrum Analysis, Raman
D003102:Colloids; D018744:DNA, Plant
DNA; Leaf tissue; Silver colloidal nanoparticles; Surface-enhanced Raman scattering (SERS); Tomato cultivars