Abundance and diversity of methanogens: potential role in high arsenic groundwater in Hetao Plain of Inner Mongolia, China.
To investigate the community diversity and abundance of methanogens and their potential role in high arsenic groundwater, 17 groundwater samples from Hetao Plain of Inner Mongolia were investigated with an integrated method including 16S rRNA gene clone library, quantitative polymerase chain reaction and geochemistry analyses. Total arsenic (AsTot) concentrations were 82.7-1088.7 μg/L and arsenite (AsIII) mostly dominated in these samples with percentages of 0.04-0.79. CH₄ concentrations ranged from 0.01 to 292 μg/L and distinctly elevated only when AsTot were relatively high and SO₄(2-) were distinctly low. Principal component analysis indicated that these samples were divided into three groups according to the variations of AsTot, CH₄ and SO₄(2-). AsTot concentrations were distinctly high in the group with high CH₄ and low SO₄(2-) comparing to the other two groups (one with high CH₄ and high SO₄(2-), the other with low CH₄ and SO₄(2-)). The mcrA gene (methyl coenzyme-M reductase gene) based phylogenetic analysis of methanogens population showed that methanogenic archaea was diverse but mainly composed of Methanomicrobiales, Methanosarcinales, Methanobacteria and unidentified groups, with Methanomicrobiales being distinctly dominant (50.6%). The mcrA gene abundance in high arsenic groundwater ranged from 3.01 × 10(3) to 3.80 × 10(6)copies/L and accounted for 0-30.2% of total archaeal 16S rRNA genes. The abundance of mcrA genes was positively correlated with the concentrations of AsTot (R=0.59), AsIII (R=0.57) and FeII (R=0.79), while it was negatively correlated with oxidation-reduction potential (R=-0.66) and SO₄(2-) concentration (R=-0.64). These results implied that methanogenic archaea might accelerate As release in groundwater aquifers in Hetao Plain.
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China.
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Wang
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China. Electronic address: pli@cug.edu.cn.
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Li
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China.
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Dai
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China.
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Zhang
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China.
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Jiang
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China.
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Jiang
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China. Electronic address: yx.wang@cug.edu.cn.
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Wang
D001105:Archaea; D001151:Arsenic / Q000032:analysis*; D002681:China; D060587:Groundwater / Q000737:chemistry / Q000382:microbiology*; D019638:Methanomicrobiaceae / Q000145:classification*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*
D014874:Water Pollutants, Chemical; D001151:Arsenic
High arsenic groundwater; Inner Mongolia; Methanogen diversity; mcrA; qPCR