Staphylococcus aureus Inhibits Neutrophil-derived IL-8 to Promote Cell Death.
While Staphylococcus aureus accelerates human neutrophil cell death, the underlying host- and pathogen-derived mechanisms remain incompletely defined. Previous studies demonstrated that the S. aureus SaeR/S sensory system is essential for pathogen survival following neutrophil phagocytosis. Herein, we demonstrate that the SaeR/S system promoted accelerated cell death, suppressed phosphorylation of nuclear factor-κB, and reduced interleukin-8 (IL-8) production in human neutrophils. Treatment of neutrophils with recombinant IL-8 significantly reduced bacterial burden and apoptosis. Our findings demonstrate a mechanism by which S. aureus suppresses the early neutrophil-derived IL-8 response to disrupt cell fate and promote disease.
Department of Microbiology and Immunology, Montana State University, Bozeman.
Oliwia W
OW
Zurek
Department of Microbiology and Immunology, Montana State University, Bozeman.
Kyler B
KB
Pallister
Department of Microbiology and Immunology, Montana State University, Bozeman.
Jovanka M
JM
Voyich
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology*; D014157:Transcription Factors
D001426:Bacterial Proteins; D016209:Interleukin-8; D016328:NF-kappa B; C433805:SaeR protein, Staphylococcus aureus; D014157:Transcription Factors; D011494:Protein Kinases; C527351:SaeS protein, Staphylococcus aureus
IL-8; NF-κB; SaeR/S; Staphylococcus aureus; cell fate; neutrophils; pathogenesis