Levels of PBDEs in plasma of juvenile starlings (Sturnus vulgaris) from British Columbia, Canada and assessment of PBDE metabolism by avian liver microsomes.
In this study, the levels of polybrominated diphenyl ethers (PBDEs), HO-PBDEs, and bromophenols were monitored in starling chick plasma samples collected in Delta (British Columbia, Canada) close to the Vancouver municipal landfill and in Glen Valley, a rural area in British Columbia. The in vitro formation of hydroxylated metabolites of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) was also investigated using starling chick liver microsomes. Total PBDE plasma levels were approximately 60 times higher in starling chicks from Delta than from Glen Valley, suggesting that the Delta site is a major source of PBDEs for the local population of starlings and that PBDEs previously measured in starling eggs are bioavailable to chicks. In both locations, BDE-47 and BDE-99 were the two major congeners present at similar concentrations, suggesting contamination with the Penta-BDE mixture. Among the several possible hydroxylated metabolites of PBDEs monitored in starling plasma, only 2,4,5-tribromophenol was detected and its levels did not exceed 18±7 pg/mL. Also, several hydroxylated metabolites of BDE-47 and BDE-99 were formed by starling chick liver microsomes, but in low amounts. Therefore, our data consistently suggest that oxidative metabolism of PBDEs in starling chicks proceeds at low rate in vivo and in vitro. In conclusion, the landfill located in Delta is a relevant source of bioavailable PBDEs for the local starling population. Because of the limited ability of starling chicks to metabolize PBDEs, these compounds are likely to bioaccumulate in starlings over time. The possible toxicological implications of PBDEs bioaccumulation in starlings are currently unknown and require further research.
The University of British Columbia, Faculty of Pharmaceutical Sciences, 2405 Wesbrook Mall, Vancouver, British Columbia, Canada; Toxicological Center, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
Claudio
C
Erratico
Simon Fraser University, Department of Biological Sciences, 8888 University Dr., Burnaby, British Columbia, Canada.
Heidi
H
Currier
The University of British Columbia, Faculty of Pharmaceutical Sciences, 2405 Wesbrook Mall, Vancouver, British Columbia, Canada.
András
A
Szeitz
The University of British Columbia, Faculty of Pharmaceutical Sciences, 2405 Wesbrook Mall, Vancouver, British Columbia, Canada.
Stelvio
S
Bandiera
Toxicological Center, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
Adrian
A
Covaci
Environment Canada, 5421 Robertson Road, Delta, British Columbia, Canada. Electronic address: john.elliott@ec.gc.ca.
John
J
Elliott
D000818:Animals; D001955:British Columbia; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000097:blood / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000097:blood / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D046749:Starlings / Q000378:metabolism
D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers
HO-PBDEs; In vitro metabolism; Liver microsomes; PBDEs; Starling chicks