Sensitive single quadrupole LC/MS method for determination of lapatinib in human plasma.
A sensitive liquid chromatographic-single quadrupole mass spectrometric method was developed and validated for the determination of lapatinib in human plasma. Following a liquid-liquid extraction with methyl t-butyl ether, lapatinib and isotope labelled lapatinib, used as an internal standard (IS), were separated from the endogenous compounds on a Zorbax SB-C18 (150 x 3 mm, 3.5 μm) column. An isocratic elution with the mobile phase consisting of formic buffer and the mixture of acetonitrile, methanol and formic acid was used. Mass spectrometry with positive electrospray ionization in a single ion monitoring mode was applied. The proposed method provides the satisfactory recovery of lapatinib from human plasma and a sensitivity comparable to numerous tandem mass spectrometric methods, with a lower limit of quantification of 5 ng/mL. The validated method may be applied to pharmacokinetic studies in humans following a single 250 mg oral dose.
Jacek
J
Musijowski
Monika
M
Filist
Piotr J
PJ
Rudzki
D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D000077341:Lapatinib; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry / Q000379:methods*; D011799:Quinazolines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results
D000970:Antineoplastic Agents; D011799:Quinazolines; D000077341:Lapatinib