Comparative study of behavioural tests in the SOD1G93A mouse model of amyotrophic lateral sclerosis.
In preclinical trials, a sensitive functional test is required to detect changes in the motor behaviour of the SOD1G93A mouse model of amyotrophic lateral sclerosis (ALS). We evaluated changes in body weight and motor impairment in behavioural tests, such as the rotarod, the hanging-wire test and the treadmill, of transgenic and wild type mice. We found differences in detection of the onset of symptoms and progression of the disease between the different tests assessed. Moreover, the data showed significant gender differences in the motor behaviour of this mouse model. The rotarod and the hanging-wire test were more sensitive to detect early motor impairment. Moreover, the results suggested that the rotarod and hanging-wire became the most accurate tests rather than treadmill to characterise the ALS disease phenotype.
LAGENBIO-I3A, Facultad de Veterinaria, IIS Aragón, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Sara
S
Oliván
Ana Cristina
AC
Calvo
Amaya
A
Rando
María Jesús
MJ
Muñoz
Pilar
P
Zaragoza
Rosario
R
Osta
D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000503:physiopathology* / Q000523:psychology; D000818:Animals; D000067290:Behavior Rating Scale; D001522:Behavior, Animal; D001835:Body Weight / Q000235:genetics; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D008297:Male; D008822:Mice, Transgenic; D009043:Motor Activity / Q000235:genetics*; D009154:Mutation; D045442:Rotarod Performance Test; D012727:Sex Characteristics; D013482:Superoxide Dismutase / Q000235:genetics*
C109415:SOD1 G93A protein; D013482:Superoxide Dismutase