Reducing isozyme competition increases target fatty acid accumulation in seed triacylglycerols of transgenic Arabidopsis.
One goal of green chemistry is the production of industrially useful fatty acids (FAs) in crop plants. We focus on hydroxy fatty acids (HFAs) and conjugated polyenoic FAs (α-eleostearic acids [ESAs]) using Arabidopsis (Arabidopsis thaliana) as a model. These FAs are found naturally in seed oils of castor (Ricinus communis) and tung tree (Vernicia fordii), respectively, and used for the production of lubricants, nylon, and paints. Transgenic oils typically contain less target FA than that produced in the source species. We hypothesized that competition between endogenous and transgenic isozymes for substrates limits accumulation of unique FAs in Arabidopsis seeds. This hypothesis was tested by introducing a mutation in Arabidopsis diacylglycerol acyltransferase1 (AtDGAT1) in a line expressing castor FA hydroxylase and acyl-Coenzyme A:RcDGAT2 in its seeds. This led to a 17% increase in the proportion of HFA in seed oil. Expression of castor phospholipid:diacylglycerol acyltransferase 1A in this line increased the proportion of HFA by an additional 12%. To determine if our observations are more widely applicable, we investigated if isozyme competition influenced production of ESA. Expression of tung tree FA conjugase/desaturase in Arabidopsis produced approximately 7.5% ESA in seed lipids. Coexpression of VfDGAT2 increased ESA levels to approximately 11%. Overexpression of VfDGAT2 combined with suppression of AtDGAT1 increased ESA accumulation to 14% to 15%. Our results indicate that isozyme competition is a limiting factor in the engineering of unusual FAs in heterologous plant systems and that reduction of competition through mutation and RNA suppression may be a useful component of seed metabolic engineering strategies.
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (H.v.E., N.D.A., J.B.);Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, Louisiana 70124 (J.S.); andDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312 (M.Z.).
Harrie
H
van Erp
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (H.v.E., N.D.A., J.B.);Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, Louisiana 70124 (J.S.); andDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312 (M.Z.).
Jay
J
Shockey
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (H.v.E., N.D.A., J.B.);Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, Louisiana 70124 (J.S.); andDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312 (M.Z.).
Meng
M
Zhang
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (H.v.E., N.D.A., J.B.);Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, Louisiana 70124 (J.S.); andDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312 (M.Z.).
Neil D
ND
Adhikari
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (H.v.E., N.D.A., J.B.);Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, Louisiana 70124 (J.S.); andDepartment of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312 (M.Z.) jab@wsu.edu.
John
J
Browse
D031284:Aleurites / Q000201:enzymology; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism; D053898:Biosynthetic Pathways; D002367:Ricinus communis / Q000201:enzymology; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006720:Homozygote; D007527:Isoenzymes / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D014280:Triglycerides / Q000378:metabolism*
D029681:Arabidopsis Proteins; D005227:Fatty Acids; D007527:Isoenzymes; D014280:Triglycerides; C579477:DGAT1 protein, Arabidopsis; D051048:Diacylglycerol O-Acyltransferase