Immunohistochemical labelling of cytokines in calves infected experimentally with Mycoplasma bovis.
To gain further insight into the pathogenesis of Mycoplasma bovis-associated pneumonia, cytokine expression in different pulmonary compartments was examined. The expression of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and interferon (IFN)-γ was examined immunohistochemically in the lung of 10 calves infected experimentally with M. bovis. M. bovis antigen was located in respiratory epithelial cells and within inflammatory cells in the airway lumina. Immunolabelling for TNF-α, IL-4 and IFN-γ was usually associated with inflammation, particularly in macrophages and lymphocytes in hyperplastic bronchus-associated lymphoid tissue (BALT), in thickened alveolar septa and in the bronchoalveolar exudate of infected animals. In M. bovis infection, macrophage and lymphocyte activation results in expression of a number of cytokines capable of inducing lung lesions and hyperplasia of the BALT. The cytokines examined likely play a role in pulmonary defence against M. bovis infection.
Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain. Electronic address: frodriguez@dmor.ulpgc.es.
F
F
Rodríguez
Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
P
P
Castro
Unit of Epidemiology and Preventive Medicine, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
J B
JB
Poveda
Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
A M
AM
Afonso
Unit of Veterinary Histology and Pathology, Institute for Animal Health, Veterinary School, Universidad de Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
A
A
Fernández
D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000096:biosynthesis*; D007150:Immunohistochemistry; D045682:Mycoplasma bovis; D011019:Pneumonia, Mycoplasma / Q000276:immunology / Q000473:pathology*
D016207:Cytokines
Mycoplasma bovis; calf; cytokine; immunohistochemistry