[The expression and significance of miRNA-324-3p and WNT2B in nasopharyngeal carcinoma].
OBJECTIVE
To investigate the expression and significance of miRNA-324-3p and its target gene WNT2B in tissue specimens of nasopharyngeal carcinoma (NPC) specimens.


METHODS
qRT-PCR was used to detect the expression of miRNA-324-3p and WNT2B mRNA, and Western blot was applied to assay the expression of WNT2B protein in 39 cases of NPC specimens and 21 cases of non-carcinoma epithelium. The relationship between their expression levels and clinicopathological characteristics and their correlation with clinical pathological parameters was analyzed.


RESULTS
The expression of miRNA-324-3p was significantly down-regulated decreased but WNT2B mRNA/protein increased obviously in NPC specimens (P < 0.01). A negative correlation between miRNA-324-3p and WNT2B was spotted (P < 0.05). The expression levels of these markers were closely correlated with T stage, clinic stage and cervical lymph node metastasis (P < 0.05).


CONCLUSIONS
The loss of miRNA-324-3p and ectopic WNT2B might co-induce the initiation and progression of NPC.
Chao
C
Liu
Guo
G
Li
Yong
Y
Liu
Zhongwu
Z
Su
Shuling
S
Ren
Tengbo
T
Deng
Yongquan
Y
Tian
Yuanzhen
Y
Qiu
D002277:Carcinoma; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
D006023:Glycoproteins; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C115496:WNT2B protein, human; D051153:Wnt Proteins