Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins.
This chapter details procedures for (1) functionalizing macroporous silica particles with lectins, a class of proteins that have affinity for the glycan moieties on glycoproteins, and (2) utilizing the lectin-silica material for high-performance affinity chromatography (HPAC) to enrich glycoproteins from small volumes of biological sample materials.
Merck & Co., 126 E. Lincoln Avenue, 2000, Rahway, NJ, 07090, USA, benjamin.mann@merck.com.
Benjamin F
BF
Mann
D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D037102:Lectins / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
D006023:Glycoproteins; D037102:Lectins; D012822:Silicon Dioxide