Genetic characterization of Moniezia species in Senegal and Ethiopia.
Genetic diversity of Moniezia spp. from domestic ruminants in Senegal and Ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as Moniezia benedeni. On the other hand, the tapeworms obtained from sheep and goat were identified as Moniezia expansa or could not be identified because of the lack of diagnostic morphologic character, i.e. interproglottidal glands (IPGs). Phylogenetic analysis based on cox1 gene sequences revealed that the worms from sheep/goat and cattle formed distinct clades, and three mitochondrial lineages were confirmed within the sheep/goat tapeworms. The maximum pairwise divergences among the three mitochondrial linages were about 3% in cox1 and 0.1% in SSU rDNA, while that between the worms from sheep/goat and cattle reached 13% in cox1 and 2.7% in SSU rDNA. All of the three mitochondrial lineages contained tapeworms morphologically identified as M. expansa, and the tapeworms without IPGs were confirmed in one of the three lineages, indicating the tapeworms without IPGs were also M. expansa.
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal.
Gora
G
Diop
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510 Japan. Electronic address: yanagida@asahikawa-med.ac.jp.
Tetsuya
T
Yanagida
College of Veterinary Medicine, Haramaya University, P.O. Box 281, Dire-Dawa Ethiopia.
Zerihun
Z
Hailemariam
Department of Biology, Haramaya University, P.O. Box 138, Dire-Dawa Ethiopia.
Sissay
S
Menkir
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510 Japan.
Minoru
M
Nakao
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510 Japan.
Yasuhito
Y
Sako
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal.
Cheikh Tidiane
CT
Ba
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510 Japan.
Akira
A
Ito
D000818:Animals; D000829:Animals, Domestic; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D002589:Cestoda / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005002:Ethiopia / Q000453:epidemiology; D014644:Genetic Variation; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D008969:Molecular Sequence Data; D010802:Phylogeny; D012675:Senegal / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*
D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal
Ethiopia; Mitochondrial DNA; Moniezia; Senegal