Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing.
Coilia nasus is an anadromous kind of small to moderate size fish species, and limited transcriptomics research has been performed. In the present study, we performed Illumina sequencing to produce a 22,996,612 clean reads representing with a total of 4,599,079,601 (4.5Gb) nucleotides comprehensive transcript dataset for ovary of C. nasus. Over 20 million total reads were assembled into 63,141 unigenes, and 27,027 annotated genes were predicted by Blastx and ESTScan, respectively. Applying Blast analysis and functional annotation (e.g., GO, COG, Swissprot and KEGG), we have sampled an extensive and diverse expressed gene catalog for C. nasus representing a large proportion of the genes expressed in the ovary development process. This approach will assist in the discovery and annotation of novel genes that play key roles in anadromous fish spawning migration process.
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China. Electronic address: duanjr@ffrc.cn.
Jin-Rong
JR
Duan
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China. Electronic address: zhouyf@ffrc.cn.
Yan-Feng
YF
Zhou
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China. Electronic address: xudp@ffrc.cn.
Dong-Po
DP
Xu
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China. Electronic address: zhangmy@ffrc.cn.
Min-Ying
MY
Zhang
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China. Electronic address: liuk@ffrc.cn.
Kai
K
Liu
Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture, Yangtze River Fisheries Research Institute, CAFS, Wuhan 4302231, China. Electronic address: shiy@yfi.ac.cn.
Ying
Y
Shi
Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture, Yangtze River Fisheries Research Institute, CAFS, Wuhan 4302231, China. Electronic address: weiqw@yf.iac.cn.
Qi-Wei
QW
Wei
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Shanshui Road 9, 214128, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Xuejiali 69, 214128, China. Electronic address: fangdyan@gmail.com.
Di-An
DA
Fang
D025041:Animal Migration / Q000502:physiology*; D000818:Animals; D002681:China; D005260:Female; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D010053:Ovary / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000502:physiology*; D045483:Rivers; D059467:Transcriptome
D012313:RNA
Coilia nasus; Illumina sequencing; Ovary transcriptome; Spawning migration