Engineering a Ca⁺⁺⁺-sensitive (bio)sensor from the pore-module of a potassium channel.
Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.
Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy. mattia.difrancesco@hotmail.it.
Mattia Lorenzo
ML
DiFrancesco
Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy. sabrina.gazzarrini@unimi.it.
Sabrina
S
Gazzarrini
Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy. cristina.arrigoni@ucsf.edu.
Cristina
C
Arrigoni
Institute of Biophysics-Milan section, National Research Council, Via Celoria 26, Milano 20133, Italy. giulia.romani@unimi.it.
Giulia
G
Romani
Department of Biology, Technische Universität Darmstadt, Schnittspahnstrasse 3, Darmstadt 64287, Germany. thiel@bio.tu-darmstadt.de.
Gerhard
G
Thiel
Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy. anna.moroni@unimi.it.|Institute of Biophysics-Milan section, National Research Council, Via Celoria 26, Milano 20133, Italy. anna.moroni@unimi.it.
Anna
A
Moroni
D015374:Biosensing Techniques; D002118:Calcium / Q000737:chemistry / Q000302:isolation & purification*; D020013:Calcium Signaling / Q000502:physiology; D008564:Membrane Potentials; D015221:Potassium Channels / Q000737:chemistry* / Q000502:physiology
D015221:Potassium Channels; D002118:Calcium