Endothelial nitric oxide synthase gene polymorphism relationship with semen parameters and oxidative stress in infertile oligoasthenoteratozoospermic men.
OBJECTIVE
To assess endothelial nitric oxide synthase (eNOS) gene polymorphism relationship with semen parameters and oxidative stress in infertile oligoasthenoteratozoospermic (OAT) men.


METHODS
Three hundred subjects were divided into healthy fertile men (n = 80) and infertile OAT men (n = 220). They were subjected to history taking, clinical examination, and semen analysis in addition to malondialdehyde and glutathione peroxidase estimation in seminal plasma. Polymorphisms of eNOS G894T and T786C genotypes in peripheral blood were identified by the polymerase chain reaction-restriction fragment length polymorphism analysis.


RESULTS
Comparing infertile OAT men with fertile controls, eNOS genotype G894T demonstrated prevalence of 36.8% vs 50% for wild type (GG), 35.0% vs 47.5% for heterozygous type (GT), and 28.2% vs 2.5% for mutant homozygous type (TT). Compared with GG homozygotes, carriers with A allele exhibited >1.716-fold increased risk of OAT occurrence. Comparing infertile OAT men with fertile controls, eNOS genotype T786C demonstrated prevalence of 37.3% vs 51.3% for wild type (TT), 32.7% vs 45% for heterozygous type (TC), and 30% vs 3.7% for mutant type (CC) with significant differences. Compared with TT homozygotes, carriers with C allele exhibited >1.769-fold increased risk of OAT occurrence. G894T and T786C genotypes demonstrated significant negative correlation with sperm count, total sperm motility, sperm normal forms, and seminal glutathione peroxidase, and significant positive correlation with seminal malondialdehyde. G894T genotype demonstrated significant positive correlation with T786C genotype.


CONCLUSIONS
There is a significant relationship between eNOS genotypes T786C, G894T polymorphisms with decreased sperm parameters and increased seminal oxidative stress.
Department of Andrology, Sexology & STDs, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: taymour1155@link.net.
Taymour
T
Mostafa
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Laila A
LA
Rashed
Department of Andrology, Sexology & STDs, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
Nashaat
N
Nabil
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Hanan
H
Fouad
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Dina
D
Sabry
Department of Andrology, Sexology & STDs, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
Dalia M
DM
El-Saied
D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics*; D006801:Humans; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic; D055101:Semen Analysis
D052250:Nitric Oxide Synthase Type III