[A role for calreticulin in functioning of mitochondrial ATP-dependent potassium channel].
Homology of the amino acid sequence of the mitochondrial potassium-transporting protein (MW 57kDa), having the properties of a channel subunit of the mitochondrial ATP-dependent potassium channel, and calreticulin (MW 55kDa) was detected by MALDI-TOF-TOF analysis method. Inhibitory analysis of ATP-dependent potassium transport in mitochondria with polyclonal antibodies to calreticulin was carried out. A dose-dependent inhibition of potassium transport in mitochondria by these antibodies was shown. The maximum value of inhibition was 55-60%. Based on these data it is hypothesized that at least two types of ATP-sensitive potassium channels are localized in mitochdndrial membrane. It is expected that the type of mitochondrial ATP-dependent potassium channel, which includes homologous calreticulin protein is localized mainly at mitochondrial and reticulum membrane contact sites.
M I
MI
Shigaeva
E Iu
EIu
Talanov
N I
NI
Venediktova
S V
SV
Murzaeva
G D
GD
Mironova
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D064032:Calbindin 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000906:Antibodies; C576512:Calb2 protein, rat; D064032:Calbindin 2; D027682:Cation Transport Proteins; D024101:Mitochondrial Proteins; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase