Crystallographic structure of Ni-Co coating on the affinity adsorption of histidine-tagged protein.
The principle of immobilized metal affinity chromatography (IMAC) has been recently implemented for protein microarrays for the study of protein abundance and function. Ni-Co film fabricated by electrodeposition is a novel microarray surface in an alloy type for immobilizing histidine-tagged proteins based on IMAC. In this paper, the effects of crystallographic structures and surface properties of Ni-Co coatings, with and without the annealing process, on the immobilization of histidine-tagged proteins were systematically investigated. The experimental results reveal that the stronger hcp texture, due to a higher Co content, results in better affinity adsorption for histidine-tagged biotin. Nevertheless, the allotropic phase transformation from hcp to fcc, due to the annealing process, leads to the decrease of affinity adsorption. The wettability property and the surface roughness of Ni-Co coating are, however, not important factors. Obviously, the crystallographic structure of Ni-Co coating is the dominant factor for the specific affinity adsorption of histidine-tagged protein.
Department of Mechanical Engineering, Chung Yuan Christian University, Chung Li 32023, Taiwan; Center for Biomedical Technology (CBT), Chung Yuan Christian University, Chung Li 32023, Taiwan. Electronic address: justin@cycu.edu.tw.
Yaw-Jen
YJ
Chang
Department of Mechanical Engineering, Chung Yuan Christian University, Chung Li 32023, Taiwan.
Sheng-Zheng
SZ
Chen
Department of Mechanical Engineering, Chung Yuan Christian University, Chung Li 32023, Taiwan.
Ching-Yuan
CY
Ho
D000327:Adsorption; D000497:Alloys / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002232:Carbocyanines; D002846:Chromatography, Affinity; D005456:Fluorescent Dyes; D006639:Histidine / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation; D011506:Proteins / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
D000497:Alloys; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009842:Oligopeptides; D011506:Proteins; C085321:cyanine dye 5; D006639:Histidine; D001710:Biotin; D019809:Streptavidin
Affinity adsorption; Crystallographic structure; Electrodeposition; Histidine; Immobilized metal affinity chromatography (IMAC)