Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei).
Litopenaeus vannamei is the most important farmed shrimp species globally, but its production is affected by several factors, including infectious disease. White spot syndrome virus (WSSV), in particular, causes significant shrimp losses. To understand the shrimp's immune response against WSSV, we cloned LvAV from L. vannamei and analyzed its expression pattern in different tissues, in addition to its expression following infection. We employed dsRNA and recombinant (r)LvAV to explore the potential role of LvAV in shrimp immunity when infected with WSSV. We find that LvAV is a C-type Lectin composed of 176 amino acids with a signal peptide and a specific C-type Lectin-type domain (CTLD). It shares 81% amino acid similarity with PmAV, an antiviral-like C-type Lectin from Penaeus monodom, and it is highly expressed in the hepatopancreas. Its expression is affected by infection with both WSSV and V. parahaemolyticus. Significantly, injection with rLvAV slowed WSSV replication, while injection with LvAV dsRNA initially led to enhanced virus propagation. Surprisingly, LvAV dsRNA subsequently led to a dramatic decrease in viral load in the later stages of infection, suggesting that LvAV may be subverted by WSSV to enhance viral replication or immune avoidance. Our results indicate that LvAV plays an important, but potentially complex role in the Pacific white shrimp's immune defense.
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Shulin
S
He
Department of Politics Teaching Office, Military Economics Academy of Airforce, Wuhan 430035, China.
Lei
L
Song
School of Resource & Environmental Management, Guizhou University of Finance and Economics, Guizhou 550025, China.
Zhaoying
Z
Qian
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Fujun
F
Hou
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yongjie
Y
Liu
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Xianzong
X
Wang
Fisheries College, Guangdong Ocean University, Guangdong 524088, China.
Zhangming
Z
Peng
Fisheries College, Guangdong Ocean University, Guangdong 524088, China.
Chengbo
C
Sun
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: liuxiaolin@nwsuaf.edu.cn.
Xiaolin
X
Liu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology*; D043143:Hepatopancreas / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000276:immunology*; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics; D019562:Viral Load / Q000235:genetics; D014779:Virus Replication / Q000235:genetics; D046848:White spot syndrome virus 1 / Q000502:physiology*
D037181:Lectins, C-Type; D012330:RNA, Double-Stranded
C-type lectin; Litopenaeus vannamei; LvAV; Vibrio parahaemolyticus; WSSV