Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation.
Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M₀) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamma. In the M₁ generation, 2999 single plants were harvested. The next season, these seeds were bulked and planted to obtain M₂ progeny. The seeds of 1900 M₂ plants were picked separately. Salinity tolerance was tested in the M₃ generation. Among M₃ plantlets, 10 salt-tolerant tobacco mutants were selected. According to the results of the selection studies, 100- and 200-Gy gamma radiation doses were the effective doses to obtain the desired mutants. Glutathione reductase enzyme activities of salt-tolerant tobacco mutants were determined biochemically as a stress-tolerance marker. The differences between control and salt-tolerant mutants belonging to the Akhisar 97 and İzmir Özbaş tobacco varieties were evaluated by random amplified polymorphic DNA analysis. The total polymorphism rate was 73.91%.
Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey ocelik@iku.edu.tr.
Ö
Ö
Çelik
Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey.
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Atak
D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D018525:Germination; D005980:Glutathione Reductase / Q000378:metabolism; D016296:Mutagenesis; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000528:radiation effects*
D017931:DNA Primers; D018744:DNA, Plant; D005980:Glutathione Reductase