Cis- and cell-type-dependent trans-requirements for Lassa virus-like particle production.
Lassa virus (LASV) small zinc-finger protein (Z), which contains two L-domain motifs, plays a central role in virus budding. Here, we report that co-expression of glycoprotein (GPC) altered the requirements for cholesterol but not the L-domains and host factor, Tsg101, for Z-induced virus-like particle (VLP) production. In particular, the cholesterol requirement for VLP production was cell-type-dependent. In addition, GPC was found to be important for co-localization of Z with CD63, a late endosomal marker. We also found that the N-terminal region (aa 3-10) of Z was critical for its myristoylation and VLP production. These findings will contribute to our understanding of LASV assembly and budding.
1Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Shuzo
S
Urata
1Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan 2The Graduate School of Biomedical Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Jiro
J
Yasuda
D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D007836:Lassa virus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D022701:Virosomes / Q000235:genetics* / Q000378:metabolism*; D019065:Virus Assembly; D057074:Virus Release
D014764:Viral Proteins; D022701:Virosomes; D002784:Cholesterol