Proinflammatory cytokines in prostate cancer development and progression promoted by high-fat diet.
BACKGROUND
We aimed to examine whether proinflammatory cytokines participated in prostate cancer (PCa) development and progression promoted by high-fat diet (HFD).


METHODS
TRAMP (transgenic adenocarcinoma mouse prostate) mice were randomly divided into two groups: normal diet group and HFD group. Mortality rate and tumor formation rate were examined. TRAMP mice were sacrificed and sampled on the 20th, 24th, and 28th week, respectively. Levels of proinflammatory cytokines, including IL-1α, IL-1β, IL-6, and TNF-α, were tested by FlowCytomix. Prostate tissue of TRAMP mice was used for histology study.


RESULTS
A total of 13 deaths of TRAMP mice were observed, among which 3 (8.33%) were from the normal diet group and 10 (27.78%) from the HFD group. The mortality rate of TRAMP mice from HFD group was significantly higher than that of normal diet group (P = 0.032). Tumor formation rate at 20th week of age of HFD group was significantly higher than that of normal diet group (P = 0.045). Proinflammatory cytokines levels, including IL-1α, IL-1β, IL-6, and TNF-α, were significantly higher in HFD TRAMP mice.


CONCLUSIONS
HFD could promote TRAMP mouse PCa development and progression with elevated proinflammatory cytokines levels. Proinflammatory cytokines could contribute to PCa development and progression promoted by HFD.
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Hua
H
Xu
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Meng-bo
MB
Hu
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Pei-de
PD
Bai
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Wen-hui
WH
Zhu
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Sheng-hua
SH
Liu
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Jun-yao
JY
Hou
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zu-quan
ZQ
Xiong
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Qiang
Q
Ding
Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Hao-wen
HW
Jiang
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D018450:Disease Progression; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology*; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha