An extract of Perilla stem inhibits Src homology phosphatase-1 (SHP)-1 and influences insulin signaling.
Protein tyrosine phosphatases (PTPs) are enzymes that catalyze protein tyrosine dephosphorylation of which Src homology phosphatase-1 (SHP-1) is one of the best-validated, a widely distributed intracellular tyrosine phosphatase that contains two SH2 domains. Down regulation of SHP-1 tyrosine phosphatases was significantly increased sensitivity to insulin in insulin signaling pathway. Through in vitro enzymatic reaction kinetics experiment, we found that the extract of Perilla stem was a potential inhibitor to δSHP-1, the catalytic domain of SHP-1 protein tyrosine phosphatase, and its IC(50) was 4ug/ml, and was more sensitive towards SHP-1than other PTPs, which indicated that SHP-1 might be a target of the extract of Perilla stem. It can strengthened the level of tyrosine phosphorylation of insulin receptor (IR) and extracellular signal-regulated protein kinase (ERK) in HepG2 cells, and then activated the insulin signaling pathway through inhibiting the protein phosphorylation of SHP-1. These results demonstrated that the extract of Perilla stem could play an important role for diabetes treatment through inhibiting the level of SHP-1 in insulin signaling pathway.
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Liu
L
Peng
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Zhang
Z
Lei
The First Hospital of Jilin University, Changchun, PR China.
Xie
X
Xiao-na
The First Hospital of Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Wang
W
Deli
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Sun
S
Jing
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Wang
W
Yong-sen
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China.
Wang
W
Zhi
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Xing
X
Shu
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Ma
M
Jun-feng
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Li
L
Wan-nan
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China / The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China.
Fu
F
Xue-qi
D056945:Hep G2 Cells; D006801:Humans; D007328:Insulin / Q000502:physiology*; D031343:Perilla; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects*
D007328:Insulin; D010936:Plant Extracts; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6