The potential protective role of the combination of IL-22 and TNF-α against genital tract Chlamydia trachomatis infection.
Th22 cells are a novel class of lymphocytes characterized by the secretion of both IL-22 and TNF-α. In summary, Th22 cells have little or no direct impact on other immune cells, but exert selective effects on epithelia. It is not known, however, whether Th22 cells play a role in genital mucosal immunity. Here, we demonstrate that IL-22 and TNF-α synergistically induce several immunomodulatory molecules, such as the antimicrobial peptide mBD-2 (murine β-defensin 2) and the antimicrobial chemokines CXCL-9, -10, and -11 in primary murine oviduct epithelial cells (MOECs). The induction of innate immunity is relevant in an in vitro infection model, in which MOECs stimulated with Th22 cell supernatants or recombinant IL-22 and TNF-α effectively inhibit the growth of Chlamydia trachomatis and maintain the survival of the epithelia compared with IL-22 or TNF-α alone. In summary, we demonstrate that the Th22 cell cytokines IL-22 and TNF-α play important roles in genital tract infection. The potential for Th22 cell cytokines to modulate innate immune mediators may lead to the development of new topical agents to treat and/or prevent immune-mediated sexually transmitted diseases (STDs). In summary, we demonstrate that IL-22 and TNF-α represent a potent, synergistic cytokine combination for inducing genital mucosal immunity.
Department of Obstetrics and Gynecology, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, PR China.
Xiumin
X
Zhao
Department of Obstetrics and Gynecology, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, PR China.
Danyang
D
Zhu
First Affiliated Hospital, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
Jiangbin
J
Ye
First Affiliated Hospital, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
Xingqun
X
Li
Department of Microbiology and Immunology, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
Zhibin
Z
Wang
Department of Microbiology and Immunology, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
Lifang
L
Zhang
Department of Microbiology and Immunology, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China. Electronic address: wenxu.cn@gmail.com.
Wen
W
Xu
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D016764:Cell Polarity / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D002692:Chlamydia trachomatis / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D005835:Genitalia / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D007378:Interleukins / Q000494:pharmacology*; D008807:Mice, Inbred BALB C; D010057:Oviducts / Q000473:pathology; D054367:Receptors, CXCR3 / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D000097043:Interleukin-22
D023181:Antimicrobial Cationic Peptides; D018925:Chemokines; D004268:DNA-Binding Proteins; D007378:Interleukins; D054367:Receptors, CXCR3; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22; C515188:Cxcr3 protein, mouse; C489326:Mbd2 protein, mouse; C415369:interleukin-22 receptor
Chemokine; Defensin; IL-22; Oviduct epithelial cell; Th22 cell