Electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in the injured spinal cord of adult rats.
A contusive model of spinal cord injury at spinal segment T8-9 was established in rats. Huantiao (GB30) and Huatuojiaji (Ex-B05) were punctured with needles, and endogenous neural stem cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) and NG2. Double immunofluorescence staining showed that electroacupuncture markedly increased the numbers of BrdU(+)/NG2(+) cells at spinal cord tissue 15 mm away from the injury center in the rostral and caudal directions. The results suggest that electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in rats with spinal cord injury.
Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Haiying
H
Wu
Kunming University, Kunming 650118, Yunnan Province, China.
Min
M
Hu
Kunming University, Kunming 650118, Yunnan Province, China.
Dekai
D
Yuan
Department of Otolaryngology, Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China.
Haiying
H
Wu
Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Yunhui
Y
Wang
Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Jing
J
Wang
Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Tao
T
Li
Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Chuanyun
C
Qian
Department of Minimally Invasive Neurosurgery, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
Hualin
H
Yu
electroacupuncture; endogenous oligodendrocyte progenitor cells; neural regeneration; proliferation; remyelination; spinal cord injury