Administration of Fenugreek Seed Extract Produces Better Effects in Glibenclamide-Induced Inhibition in Hepatic Lipid Peroxidation: An in vitro Study.
OBJECTIVE
To evaluate the comparative effects of fenugreek (Trigonella foenum graecum) seed extract (FSE) alone and in combination with an antidiabetic conventional medicine, glibenclamide (GLB), on the inhibition of in vitro lipid peroxidation (LPO) in liver, the major target organ of a drug.


METHODS
LPO was induced by ferrous sulphate (FeSo4), hydrogen peroxide (H2O2) and carbon tetrachloride (CCl4) and the effects of test seed extract and/or GLB were evaluated.


RESULTS
While FeSo4, H2O2and CCl4markedly enhanced the hepatic LPO, simultaneous administration of FSE reduced it in a concentration dependent manner. However, when both FSE and GLB were added to the incubation mixture, chemically induced hepatic LPO was further inhibited. The test extract also exhibited high antioxidative activity in 1,1-diphenyl-2-picrylhydrazyl radical and in 2,2'-azinobis, 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging assays.


CONCLUSIONS
FSE therapy in moderate concentration along with a hypoglycemic drug may prove to be advantageous in ameliorating diabetes mellitus and other diseases that are LPO mediated.
School of Life Sciences, Devi Ahilya University, Takshashila Campus, Indore, 452017, India. nehasharma.908@gmail.com.
Sharma
S
Neha
School of Life Sciences, Devi Ahilya University, Takshashila Campus, Indore, 452017, India.
Kar
K
Anand
School of Life Sciences, Devi Ahilya University, Takshashila Campus, Indore, 452017, India.
Panda
P
Sunanda
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D005296:Ferrous Compounds; D016166:Free Radical Scavengers / Q000494:pharmacology; D005905:Glyburide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology*; D051381:Rats; D013451:Sulfonic Acids / Q000737:chemistry; D029910:Trigonella
D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C020748:ferrous sulfate; C056164:fenugreek seed meal; C004931:1,1-diphenyl-2-picrylhydrazyl; D005905:Glyburide
antioxidant activity; fenugreek; glibenclamide; lipid peroxidation