[Construction and characterization of a novel recombinant retroviral vector expressing mouse T-bet].
In order to study T-bet function in mouse cells, a novel retroviral vector expressing mouse T-bet and reporter gene Thy1.1 was constructed. Retrovirus particles were then produced by transfection of the recombinant retroviral plasmid into a packaging cell line Platinum-E. The recombinant retrovirus played considerable infection ability. T-bet expression was then identified by FACS after infection of CD4+ primary T cells from T-bet knockout mouse with recombinant retrovirus. To determine if exogenous expressing T-bet has normal function, we checked the expression level of T-bet target gene, Ifng. IFN-y expression was upregulated in the T-bet knockout T cells infected with recombinant retrovirus. In conclusion, we successfully constructed an effective mouse T-bet recombinant retroviral vector.
Xuejie
X
Zhang
Jianhua
J
Zhang
Wei
W
Zhang
Jie
J
Guo
Xuyu
X
Zhou
D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D011994:Recombinant Proteins / Q000096:biosynthesis; D012190:Retroviridae; D020825:T-Box Domain Proteins / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection; D000098815:T-bet Transcription Factor
D011994:Recombinant Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D007371:Interferon-gamma