S. cerevisiae × S. eubayanus interspecific hybrid, the best of both worlds and beyond.
Saccharomyces pastorianus lager-brewing yeasts have descended from natural hybrids of S. cerevisiae and S. eubayanus. Their alloploidy has undoubtedly contributed to successful domestication and industrial exploitation. To understand the early events that have led to the predominance of S. pastorianus as lager-brewing yeast, an interspecific hybrid between S. cerevisiae and S. eubayanus was experimentally constructed. Alloploidy substantially improved the performance of the S. cerevisiae × S. eubayanus hybrid as compared to either parent regarding two cardinal features of brewing yeasts: tolerance to low temperature and oligosaccharide utilization. The hybrid's S. eubayanus subgenome conferred better growth rates and biomass yields at low temperature, both on glucose and on maltose. Conversely, the ability of the hybrid to consume maltotriose, which was absent in the S. eubayanus CBS12357 type strain, was inherited from its S. cerevisiae parent. The S. cerevisiae × S. eubayanus hybrid even outperformed its parents, a phenomenon known as transgression, suggesting that fast growth at low temperature and oligosaccharide utilization may have been key selective advantages of the natural hybrids in brewing environments. To enable sequence comparisons of the parental and hybrid strains, the genome of S. eubayanus CBS12357 type strain (Patagonian isolate) was resequenced, resulting in an improved publicly available sequence assembly.
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands Netherlands Consortium for Systems Biology, 1098 XH Amsterdam, the Netherlands.
Marit
M
Hebly
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.
Anja
A
Brickwedde
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.
Irina
I
Bolat
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.
Maureen R M
MR
Driessen
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.
Erik A F
EA
de Hulster
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.
Marcel
M
van den Broek
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands Netherlands Consortium for Systems Biology, 1098 XH Amsterdam, the Netherlands Platform Green Synthetic Biology, 2628 BC Delft, the Netherlands.
Jack T
JT
Pronk
Heineken Global Supply Chain, Technology & Policies, 2382 PH Zoeterwoude, the Netherlands.
Jan-Maarten
JM
Geertman
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands Platform Green Synthetic Biology, 2628 BC Delft, the Netherlands.
Jean-Marc
JM
Daran
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands Netherlands Consortium for Systems Biology, 1098 XH Amsterdam, the Netherlands p.a.s.daran-lapujade@tudelft.nl.
Pascale
P
Daran-Lapujade
D000434:Alcoholic Beverages / Q000382:microbiology; D002678:Chimera / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D003433:Crosses, Genetic; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D009844:Oligosaccharides / Q000378:metabolism; D011003:Ploidies; D012440:Saccharomyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D013696:Temperature
D003470:Culture Media; D009844:Oligosaccharides
Saccharomyces cerevisiae; Saccharomyces eubayanus; Saccharomyces pastorianus; genome sequence; interspecific hybrid; maltotriose; temperature