Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence.
Oropouche virus (OROV) is a public health threat in South America, and in particular in northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil, in 2009. We also report the complete genome sequences of two OROV reassortants isolatd from two marmosets in Minas Gerais state, south-east Brazil, in 2012 that contained a novel M genome segment. Interestingly, all 10 isolates possessed a 947 nt S segment that lacked 11 residues in the S-segment 3' UTR compared with the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated, and the identification of yet another reassortant highlights the importance of bunyavirus surveillance in South America.
1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK 2Biomedical Sciences Research Complex, School of Biology, University of St Andrews, St Andrews KY16 9ST, Scotland, UK.
Natasha L
NL
Tilston-Lunel
1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK.
Joseph
J
Hughes
1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK 3Department of Cell and Molecular Biology, University of Sao Paulo School of Medicine, 3900, Av Bandeirantes, Ribeirão Preto, SP 14049-900, Brazil.
Gustavo Olszanski
GO
Acrani
4Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Brazil.
Daisy E A
DE
da Silva
5Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ananindeua, Brazil.
Raimunda S S
RS
Azevedo
5Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ananindeua, Brazil.
Sueli G
SG
Rodrigues
5Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ananindeua, Brazil.
Pedro F C
PF
Vasconcelos
4Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Brazil.
Marcio R T
MR
Nunes
1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK.
Richard M
RM
Elliott
D000293:Adolescent; D000328:Adult; D000818:Animals; D001938:Brazil; D002044:Bunyaviridae Infections / Q000662:veterinary / Q000821:virology; D002144:Callithrix; D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D055815:Young Adult
D012367:RNA, Viral