Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense.
Cadmium (Cd) may pose risks to freshwater organisms, including crabs that live at the interface of sediments and water column all year round. One of the major changes that occur during oocyte maturation of crabs is the production of vitellin. In the present study, the authors investigated the effects of Cd on oocyte size, vitellin level, and vitellogenin (Vtg) messenger RNA (mRNA) expression in the ovary of the freshwater crab Sinopotamon henanense. The authors studied the impacts of Cd on carbohydrate as well as on the protein metabolism, metallothionein, glutathione (GSH) synthesis, energy-related parameters, and mRNA expression of genes involved in energy metabolism. After Cd treatment, vitellin concentration, Vtg mRNA expression, and oocyte diameter decreased. Less carbohydrate and enhanced protein catabolism were found in the ovary. Adenosine triphosphate to adenosine diphosphate (ATP:ADP) ratios, reduced nicotinamide adenine dinucleotide (NADH) to oxidized nicotinamide adenine dinucleotide (NAD(+) ) ratios, mitochondrial membrane potential, and energy production-related mRNA expressions increased in the ovary after 10 d Cd treatment and decreased after 20 d. Metallothionein and GSH synthesis were up-regulated after 10 d on Cd exposure. Moreover, Cd caused a time-dependent up-regulation of malondialdehyde. The authors' findings show significant effects of Cd on vitellogenesis, which suggests that Cd slows down vitellogenesis in S. henanense because of excessive energy consumption and an activated defense system.
School of Life Science, Shanxi University, Taiyuan, Shanxi Province, People's Republic of China.
Jian
J
Yang
School of Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi Province, People's Republic of China.
Dongmei
D
Liu
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
Hans-Uwe
HU
Dahms
School of Life Science, Shanxi University, Taiyuan, Shanxi Province, People's Republic of China.
Lan
L
Wang
D000818:Animals; D003386:Brachyura / Q000187:drug effects* / Q000254:growth & development*; D002104:Cadmium / Q000633:toxicity*; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D005618:Fresh Water / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008668:Metallothionein / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects; D014818:Vitellogenesis / Q000187:drug effects*; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D002104:Cadmium; D008315:Malondialdehyde; D008668:Metallothionein; D005978:Glutathione
Energy production; Sinopotamon henanense; Vitellin; Vitellogenin