Quantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in the plasma of Wistar rats during the oral glucose tolerance test.
The purpose of this study was to gain insight into the production behavior of free adducts of advanced glycation end-products (AGEs) in Wistar rats under acute hyperglycemic conditions. Five AGE-free adducts as well as their precursors (i.e., highly reactive carbonyl intermediates of methylglyoxal and glyoxal) in rat plasma were quantitatively determined at greater than nanomolar levels using the liquid chromatography/tandem mass spectrometry method coupled with 2,4,6-trinitrobenzene sulfonate and 2,3-diaminonaphthalene derivatization techniques. An oral glucose (2 g/kg dose) tolerance test to 10-week-old Wistar rats provided evidence that the plasma levels of diabetes-related metabolites did not change acutely within 120 min, irrespective of increasing blood glucose levels.
Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyushu University.
Si Jing
SJ
Chen
Chiwa
C
Aikawa
Toshiro
T
Matsui
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005951:Glucose Tolerance Test; D017127:Glycation End Products, Advanced / Q000097:blood*; D006037:Glyoxal / Q000097:blood*; D008297:Male; D017208:Rats, Wistar
D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D006037:Glyoxal