Enantioselective apoptosis induced by individual isomers of bifenthrin in Hep G2 cells.
Bifenthrin (BF) has been used in racemate for agricultural purposes against soil insects, leading to increased inputs into soil environments. However, most of the studies about the toxicology research on BF were performed in its racemic form. The aim of the present study was to evaluate the enantiomer-specific cis-BF-induced apoptosis and intracellular reactive oxygen species (ROS) generation on human hepatocarcinoma cells (Hep G2). The results of cell viability assay and cytoflow assay indicated an obvious enantioselective hepatocyte toxicity of 1S-cis-BF in Hep G2 cells. 1S-cis-BF also induced ROS production, up-regulated Bax protein expression and down-regulated Bcl-2 expression levels. The present study suggested that enantioselective toxicity should be evaluated on currently used chiral pesticides, such as synthetic pyrethroids.
Department of Public Health, School of Medicine, China Three Gorges University, 8# Daxue Avenue, Yichang 443002, China. Electronic address: hgliu@ctgu.edu.cn.
Huigang
H
Liu
Department of Public Health, School of Medicine, China Three Gorges University, 8# Daxue Avenue, Yichang 443002, China.
Juan
J
Li
D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D011722:Pyrethrins / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013237:Stereoisomerism
D007306:Insecticides; D011722:Pyrethrins; D017382:Reactive Oxygen Species; C099952:bifenthrin
Apoptosis; Enantioselective; Pyrethroid pesticides