Characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis.
The affinity of boronic acids toward cis-diol-containing biomolecules has found wide applications in many fields, such as sensing, separation, drug delivery, and functional materials. A sound understanding of the binding interactions will greatly facilitate exquisite applications of this chemistry. Traditional techniques are associated with some apparent drawbacks, so they are only applicable to a limited range of boronic acids and cis-diol-containing biomolecules. This chapter describes an affinity capillary electrophoresis (ACE) method for the characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules. As compared with existing approaches, such as (11)B NMR, the ACE method exhibits several significant advantages: (1) possibility of simultaneous study of multiple interactions, (2) low requirement on the purity of the binding species, (3) widely applicable to almost all types of cis-diol-containing compounds and boronic acids, and (4) high accuracy and precision.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Hankou Road 22, Nanjing, 210093, China.
Chenchen
C
Lü
Zhen
Z
Liu
D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D001897:Boronic Acids / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006018:Glycols; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D001704:Biopolymers; D001897:Boronic Acids; D006018:Glycols; D009005:Monosaccharides; D009705:Nucleosides; D011506:Proteins