Short communication: Bovine-derived proteins activate STAT3 in human skeletal muscle in vitro.
Bovine milk contains biologically active peptides that may modulate growth and development within humans. In this study, targeted bovine-derived proteins were evaluated for their effects on signal transducer and activator of transcription-3 (STAT3) phosphorylation in human skeletal muscle cells. Following an acute exposure, bovine-derived acidic fibroblast growth factor-1 (FGF) and leukemia inhibitory factor (LIF) activated STAT3 in differentiating myotubes. Chronic exposure to FGF and LIF during the proliferative phase reduced myoblast proliferation and elevated MyoD and creatine kinase (CKM) mRNA expression without altering apoptotic genes. In mature myotubes, neither FGF nor LIF elicited any action. Together, these data indicate that a reduction in proliferation in the presence of bovine-derived FGF or LIF may stimulate early maturation of myoblasts.
Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne, 3125, Australia; Basic and Clinical Myology Laboratory, Department of Physiology, University of Melbourne, Melbourne, 3010, Australia. Electronic address: marissa.caldow@unimelb.edu.au.
M K
MK
Caldow
Department of Zoology, University of Melbourne, Melbourne, 3010, Australia.
M R
MR
Digby
Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne, 3125, Australia; Liggins Institute, University of Auckland, Auckland 1142, New Zealand.
D
D
Cameron-Smith
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000187:drug effects*; D032446:Myoblasts / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D053605:Leukemia Inhibitory Factor; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D016220:Fibroblast Growth Factor 1
STAT3; bovine protein; cytokine; growth factor; skeletal muscle