Microbial respiration and natural attenuation of benzene contaminated soils investigated by cavity enhanced Raman multi-gas spectroscopy.
Soil and groundwater contamination with benzene can cause serious environmental damage. However, many soil microorganisms are capable to adapt and are known to strongly control the fate of organic contamination. Innovative cavity enhanced Raman multi-gas spectroscopy (CERS) was applied to investigate the short-term response of the soil micro-flora to sudden surface contamination with benzene regarding the temporal variations of gas products and their exchange rates with the adjacent atmosphere. (13)C-labeled benzene was spiked on a silty-loamy soil column in order to track and separate the changes in heterotrophic soil respiration - involving (12)CO2 and O2- from the natural attenuation process of benzene degradation to ultimately form (13)CO2. The respiratory quotient (RQ) decreased from a value 0.98 to 0.46 directly after the spiking and increased again within 33 hours to a value of 0.72. This coincided with the maximum (13)CO2 concentration rate (0.63 μmol m(-2) s(-1)), indicating the highest benzene degradation at 33 hours after the spiking event. The diffusion of benzene in the headspace and the biodegradation into (13)CO2 were simultaneously monitored and 12 days after the benzene spiking no measurable degradation was detected anymore. The RQ finally returned to a value of 0.96 demonstrating the reestablished aerobic respiration.
Leibniz Institute of Photonic Technology, Jena, Germany. torsten.frosch@uni-jena.de torsten.frosch@gmx.de.
Tobias
T
Jochum
Beate
B
Michalzik
Anne
A
Bachmann
Jürgen
J
Popp
Torsten
T
Frosch
D001554:Benzene / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism*; D004787:Environmental Pollution / Q000032:analysis; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods
D012987:Soil; D012989:Soil Pollutants; D002245:Carbon Dioxide; D001554:Benzene; D010100:Oxygen