Selenium Deficiency Downregulates Selenoproteins and Suppresses Immune Function in Chicken Thymus.
Selenoproteins and selenium (Se) play important roles in the immune system. Selenoprotein expression in the immune system of mammals is sensitive to dietary Se levels; however, little is known about the expression of selenoproteins and their immune functions in the chicken thymus. We assessed selenoprotein gene expression and cytokine content in the chicken thymus in this study. The animals were randomly assigned to two groups as follows: the Se-deficient group (L group) was fed a diet containing 0.033 mg Se/Kg, and the control group was fed the same basal diet supplemented with Se at 0.15 mg/kg (sodium selenite). Real-time qPCR was used to investigate the expression level of selenoproteins on days 15, 25, 35, 45, and 55, and ELISA was used to evaluate the cytokine content on days 15, 35, and 55. The messenger RNA (mRNA) levels of Txnrd1, Txnrd2, Txnrd3, Dio1, Dio2, Dio3, GPx1, GPx2, GPx3, Gpx4, Sepp1, Selo, Sep15, Sepx1, Sels, Seli, Selu, Selh, and SPS2 were all significantly decreased (P < 0.05) in the L group compared to the control group. A significant decrease in IL-2, IL-10, IL-17, IL-1β, IFN-α, and IFN-β was observed in the L group, and there was also a significant increase in IL-6, IL-8, IFN-γ, and TNF-α in the L group. In summary, Se deficiency results in significant changes in the expression of selenoproteins, which may cause oxidative stress in the chicken thymus tissue. Moreover, immunological changes and immune stress may occur because of Se deficiency in the chicken thymus.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Pervez Ahmed
PA
Khoso
Zijiang
Z
Yang
Chunpeng
C
Liu
Shu
S
Li
D000818:Animals; D000831:Animals, Newborn; D030161:Avian Proteins / Q000235:genetics; D002645:Chickens; D016207:Cytokines / Q000378:metabolism; D004032:Diet; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D051140:Selenoproteins / Q000235:genetics*; D013950:Thymus Gland / Q000276:immunology* / Q000378:metabolism*; D013997:Time Factors
D030161:Avian Proteins; D016207:Cytokines; D012333:RNA, Messenger; D051140:Selenoproteins; D012643:Selenium