VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.
Mette
M
Grønlund
D016960:Agrobacterium tumefaciens / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques / Q000379:methods; D020868:Gene Silencing; D038821:Mycorrhizae / Q000502:physiology; D018532:Pisum sativum / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics* / Q000502:physiology; D016961:Rhizobium leguminosarum / Q000502:physiology; D013559:Symbiosis
D010940:Plant Proteins