Salivary, blood and plasma nitrite concentrations in periodontal patients and healthy individuals before and after periodontal treatment.
BACKGROUND
To date, no study has employed ozone-based reductive chemiluminescence to compare nitrite concentration in the saliva of periodontal disease (PD) and healthy individuals or in the various blood compartments of the same individuals before and after periodontal treatment. We evaluated nitrite concentrations in whole, submandibular, and parotid saliva, as well as in whole blood, erythrocytes, and plasma of healthy volunteers and patients with chronic periodontitis.


METHODS
Data obtained for the PD and control groups were compared before and 3 months after periodontal therapy.


RESULTS
At baseline, stimulated whole saliva nitrite concentration was lower in PD patients (mean=57.3 ± 9.8 μmol/L) as compared with healthy individuals (92.5 ± 13.6 μmol/L, P<0.05). PD and periodontal treatment did not affect submandibular or parotid saliva nitrite concentrations. PD patients presented higher baseline whole blood nitrite concentration (238.4 ± 45.7 μmol/L) as compared with values recorded 3 months after therapy (141.3 ± 20.1 nmol/L, P<0.05). PD patients' erythrocytes exhibited higher baseline nitrite concentration (573.1 ± 97.8 nmol/L) as compared with three months after therapy (298.7 ± 52.1 nmol/L, P<0.05). Again, PD and PD treatment did not impact plasma nitrite concentration.


CONCLUSIONS
PD patients had lower nitrite concentration in whole saliva, and this situation remained unchanged after periodontal treatment. Nevertheless, erythrocytes and whole blood nitrite levels diminished after periodontal treatment.
Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: cesar.meschiari@usp.br.
Cesar A
CA
Meschiari
Department of Morphology and Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Leonardo R
LR
Zuardi
Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Valeria A
VA
Gomes
Department of Morphology and Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Glauce R
GR
Costa de Almeida
Department of Oral Maxillofacial Surgery & Traumatology and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Arthur B
AB
Novaes
Department of Morphology and Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Raquel F
RF
Gerlach
School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil.
Andrea M
AM
Marcaccini
D006801:Humans; D009573:Nitrites / Q000032:analysis* / Q000097:blood*; D010510:Periodontal Diseases / Q000097:blood* / Q000188:drug therapy* / Q000378:metabolism; D010949:Plasma / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*
D009573:Nitrites
Blood; Chemiluminescence; Nitric oxide; Nitrite; Periodontal disease; Saliva