Oleiagrimonas soli gen. nov., sp. nov., a genome-sequenced gammaproteobacterium isolated from an oilfield.
A yellow-pigmented strain, designated strain 3.5X(T), was isolated from oil-contaminated saline soil in Gudao, Shandong Province, China, and was characterized taxonomically. The results showed that the isolate was a Gram-stain-negative, aerobic, motile, rod-shaped cell with a polar flagellum. Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Xanthomonadaceae in the class Gammaproteobacteria and represented an independent taxon separated from other genera. The closest relative of strain 3.5X(T) was Fulvimonas soli DSM 14263(T) (94% similarity). The genomic DNA G+C content was 67 mol% by thermal denaturation and 66.3 mol% from genome sequences. The cells mainly consisted of branched fatty acids, with iso-C17 : 0, iso-C15 : 0, iso-C17 : 1ω9c and/or C16 : 010-methyl and iso-C11 : 0 as the major fatty acids. The main polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol and phosphatidylethanolamine. In addition, ubiquinone Q-8 was the major component of the quinone system and the polyamine pattern contained the major compound spermidine plus minor amounts of putrescine and spermine. On the basis of phenotypic, genotypic and phylogenetic distinctiveness, it is proposed that the isolate represents a novel species in a novel genus, namely Oleiagrimonas soli gen. nov., sp. nov. The type strain is 3.5X(T) ( =NBRC 110685(T) =KCTC 42351(T) =CPCC 100614(T)).
School of Environment, Tsinghua University, Beijing 100084, PR China.
Tingting
T
Fang
School of Environment, Tsinghua University, Beijing 100084, PR China.
Hui
H
Wang
School of Environment, Tsinghua University, Beijing 100084, PR China.
Yong
Y
Huang
School of Environment, Tsinghua University, Beijing 100084, PR China.
Haiyan
H
Zhou
School of Environment, Tsinghua University, Beijing 100084, PR China.
Peiyan
P
Dong
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D011073:Polyamines / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8