[Expression of acylamidase gene in Rhodococcus erythropolis strains].
The expression of a new acylamidase gene from R. erythropolis 37 was studied in Rhodococcus erythropolis strains. This acylamidase, as a result of its unique substrate specificity, can hydrolyse N-substituted amides (4'-nitroacetanilide, N-isopropylacrylamide, N'N-dimethylaminopropylacrylamide). A new expression system based on the use of the promoter region of nitrilhydratase genes from R. rhodochrous M8 was created to achieve constitutive synthesis of acylamidase in R. erythropolis cells. A fourfold improvement in the acylamidase activity of recombinant R. erythropolis cells as compared with the parent wild-type strain was obtained through the use of the new expression system.
K V
KV
Lavrov
A D
AD
Novikov
L E
LE
Riabchenko
A S
AS
Ianenko
D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000581:Amidohydrolases; C019665:amidase