Establishment of primary cultures of craniopharyngioma cells.
Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research.
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Hao
H
Liu
Department of Neurosurgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
Liang
L
Liu
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Zhiyong
Z
Liu
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Qiang
Q
Li
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Chao
C
You
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Jianguo
J
Xu
cell line; craniopharyngioma; culture in vitro; cytokeratin-7; primary culture