Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy.
BACKGROUND
We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum.


METHODS
Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples.


RESULTS
Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG.


CONCLUSIONS
This study proves that VSG suppresses hepatic glucose production.
Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea.
Yunmee
Y
Lho
Diabetes Complications Research Center, UCD Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
Carel W
CW
le Roux
Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Kyeongsang National University, Jinju, Republic of Korea.
Hyeon Soo
HS
Park
Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Kyeongsang National University, Jinju, Republic of Korea.
Gon Sup
GS
Kim
KM Health Technology Research Group, Medical Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Jeeyoun
J
Jung
Integrated Metabolomics Research Group of Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea.
Geum-Sook
GS
Hwang
Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea.
Youn Kyoung
YK
Seo
Department of Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea. htksurgeon@gmail.com.
Tae Kyung
TK
Ha
Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea. hanne.md@gmail.com.
Eunyoung
E
Ha
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004195:Disease Models, Animal; D004435:Eating / Q000502:physiology; D005743:Gastrectomy / Q000379:methods; D005943:Gluconeogenesis / Q000502:physiology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism* / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015431:Weight Loss
D001786:Blood Glucose; D007328:Insulin; D005952:Glucose-6-Phosphatase
Gluconeogenesis; Metabolome; Proteome; Vertical sleeve gastrectomy