Coronavirus envelope (E) protein remains at the site of assembly.
Coronaviruses (CoVs) assemble at endoplasmic reticulum Golgi intermediate compartment (ERGIC) membranes and egress from cells in cargo vesicles. Only a few molecules of the envelope (E) protein are assembled into virions. The role of E in morphogenesis is not fully understood. The cellular localization and dynamics of mouse hepatitis CoV A59 (MHV) E protein were investigated to further understanding of its role during infection. E protein localized in the ERGIC and Golgi with the amino and carboxy termini in the lumen and cytoplasm, respectively. E protein does not traffic to the cell surface. MHV was genetically engineered with a tetracysteine tag at the carboxy end of E. Fluorescence recovery after photobleaching (FRAP) showed that E is mobile in ERGIC/Golgi membranes. Correlative light electron microscopy (CLEM) confirmed the presence of E in Golgi cisternae. The results provide strong support that E proteins carry out their function(s) at the site of budding/assembly.
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, United States; School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401, United States; Microbiology Graduate Program, Arizona State University, Tempe, AZ 85287-5401, United States.
Pavithra
P
Venkatagopalan
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, United States; Department of Biochemistry and Chemistry, Arizona State University, Tempe, AZ 85287-5401, United States.
Sasha M
SM
Daskalova
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, United States; School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401, United States; Molecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ 85287-5401, United States.
Lisa A
LA
Lopez
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, United States; School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401, United States; Microbiology Graduate Program, Arizona State University, Tempe, AZ 85287-5401, United States.
Kelly A
KA
Dolezal
The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401, United States; School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401, United States. Electronic address: Brenda.Hogue@asu.edu.
Brenda G
BG
Hogue
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000821:virology; D006056:Golgi Apparatus / Q000737:chemistry / Q000821:virology; D007425:Intracellular Membranes / Q000737:chemistry*; D051379:Mice; D008853:Microscopy; D006517:Murine hepatitis virus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000032:analysis*; D019065:Virus Assembly; D057074:Virus Release
D014759:Viral Envelope Proteins
CLEM; Coronavirus; Envelope protein; FRAP; Live-cell imaging; Protein transport/localization; Virus assembly