Selecting an indigenous microalgal strain for lipid production in anaerobically treated piggery wastewater.
The aim of this study was to select a potential microalgal strain for lipid production and to examine the suitability of anaerobically treated piggery wastewater as a nutrient source for production of lipid-rich biomass with the selected microalga. Biomass and lipid productivity of three microalgal strains (Chlorella sorokiniana CY1, Chlorella vulgaris CY5 and Chlamydomonas sp. JSC-04) were compared by using different media, nitrogen sources, and nitrogen concentrations. The highest lipid content and productivity (62.5 wt%, 162 mg/L/d) were obtained with C. vulgaris with BG-11 with 62 mg N/L. Secondly, C. vulgaris was cultivated in sterilized, diluted (1-20×), anaerobically treated piggery wastewater. Biomass production decreased and lipid content increased, when wastewater was more diluted. The highest lipid content of 54.7 wt% was obtained with 20× dilution, while the highest lipid productivity of 100.7 mg/L/d with 5× dilution. Piggery wastewater is a promising resource for mass production of oleaginous microalgal biomass.
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere 33101, Finland. Electronic address: jatta.marjakangas@tut.fi.
Jatta M
JM
Marjakangas
Center of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Chun-Yen
CY
Chen
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere 33101, Finland.
Aino-Maija
AM
Lakaniemi
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere 33101, Finland.
Jaakko A
JA
Puhakka
Department of Environmental Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Liang-Ming
LM
Whang
Center of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan.
Jo-Shu
JS
Chang
D000693:Anaerobiosis; D000818:Animals; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000378:metabolism*; D013552:Swine; D062065:Wastewater; D018508:Water Purification
D008055:Lipids; D062065:Wastewater
Chlorella vulgaris; Lipid production; Piggery wastewater