Reversible adsorption of catalase onto Fe(3+) chelated poly(AAm-GMA)-IDA cryogels.
In this presented study, poly(acrylamide-glycidyl methacrylate) [poly(AAm-GMA)] cryogels were synthesized by cryopolymerization technique at sub-zero temperature. Prepared cryogels were then functionalized with iminodiacetic acid (IDA) and chelated with Fe(3+) ions in order produce the metal chelate affinity matrix. Synthesized cryogels were characterized with FTIR, ESEM and EDX analysis, and it was found that the cryogel had sponge like structure with interconnected pores and their pore diameter was about 200 μm. Fe(3+) chelated poly(AAm-GMA)-IDA cryogels were used for the adsorption of catalase and optimum adsorption conditions were determined by varying the medium pH, initial catalase concentration, temperature and ionic strength. Maximum catalase adsorption onto Fe(3+) chelated poly(AAm-GMA)-IDA cryogel was found to be 12.99 mg/g cryogel at 25 °C, by using pH 5.0 acetate buffer. Adsorbed catalase was removed from the cryogel by using 1.0M of NaCl solution and desorption yield was found to be 96%. Additionally, reusability profile of the Fe(3+) chelated poly(AAm-GMA)-IDA cryogel was also investigated and it was found that, adsorption capacity of the cryogels didn't decrease significantly at the end of the 40 reuses. Catalase activity studies were also tested and it was demonstrated that desorbed catalase retained 70% of its initial activity.
Chemistry Department, Adnan Menderes University, Aydın, Turkey. Electronic address: daktas@adu.edu.tr.
Deniz
D
Aktaş Uygun
Koçarlı Vocational and Training School, Adnan Menderes University, Aydın, Turkey.
Murat
M
Uygun
Biochemistry Department, Ege University, İzmir, Turkey.
Sinan
S
Akgöl
Chemistry Department, Hacettepe University, Ankara, Turkey.
Adil
A
Denizli
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002374:Catalase / Q000302:isolation & purification*; D002417:Cattle; D059985:Cryogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007098:Imino Acids / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D059027:Recycling; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D000180:Acrylic Resins; D059985:Cryogels; D007098:Imino Acids; D007502:Iron Chelating Agents; D002374:Catalase; C008109:iminodiacetic acid
Catalase; Cryogel; IDA; IMAC