Central obesity and not age increases skeletal muscle lipids, without influencing lean body mass and strength.
OBJECTIVE
To measure skeletal muscle lipid infiltration, its association with insulin resistance (IR) lean mass and function, in Chilean men differing in age and body composition. Our hypothesis was that muscle lipid accumulation would be higher among older and heavier individuals and this would deteriorate insulin sensitivity (IS) and decrease muscle mass and function, both features of the ageing process.


METHODS
Healthy men (38 < 55 and 18 > 65 years), underwent anthropometric measurements, body composition assessment through radiologic densitometry, Nuclear Magnetic Resonance spectroscopy at the tibialis anterioris muscle to measure intra (IMCL) and extramyocellular lipids (EMCL), quadriceps and handgrip strength, 12 minute walking distance and serum biochemistry (haemoglobin, lipoproteins, creatinine, ultrasensitive C Reactive Protein, fasting and post glucose insulin and glucose concentrations, to assess IS). Physical activity was estimated by actigraphy.


RESULTS
23 men were eutrophic, 26 were overweight and 7 were obese and mostly sedentary, independent of age. Both IMCL and EMCL were higher in overweight/ obese men. Abdominal fat was negatively associated with IS and positively correlated with muscle lipid accretion (both IMCL and EMCL), but not with age. As expected, older individuals had lower muscle mass and strength, but not more adipose tissue nor intramyocellular lipids, yet were more glucose intolerant.


CONCLUSIONS
central obesity was associated with IMCL and EMCL infiltration and IR. This type of lipid accretion was not related with ageing nor age-related sarcopenia. Older individuals were more glucose intolerant, which was explained by a decrease of insulin secretion more than adiposity-related IR.
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
María Pía
MP
de la Maza
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Sandra
S
Hirsch
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Natalia
N
Jara
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Laura
L
Leiva
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Gladys
G
Barrera
Radiology Department, Faculty of Medicine, Clínica Alemana and Universidad del Desarrollo, Santiago de Chile. Chile.. mpmaza@inta.uchile.cl.
Claudio
C
Silva
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Loreto
L
Pañella
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Sandra
S
Henríquez
Institute of Nutrition and Food Technology, University of Chile.. mpmaza@inta.uchile.cl.
Daniel
D
Bunout
D056044:Actigraphy; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000886:Anthropometry; D001823:Body Composition; D015992:Body Mass Index; D002677:Chile; D005260:Female; D018149:Glucose Intolerance / Q000453:epidemiology / Q000378:metabolism; D018737:Hand Strength; D006801:Humans; D007333:Insulin Resistance; D008055:Lipids / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000473:pathology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D056128:Obesity, Abdominal / Q000378:metabolism*; D012016:Reference Values
D008055:Lipids