[CXCL12α and CXCL12β stimulate the migration of cultured LX-2 hepatic stellate cells].
OBJECTIVE
To investigate the influence of chemokine (C-X-C motif) ligand 12α (CXCL12α) and CXCL12β on migration of hepatic stellate cells (HSCs).


METHODS
The cultured human LX-2 HSCs in vitro were divided into control group, platelet-derived growth factor-BB (PDGF-BB) group (10 ng/mL), CXCL12α group (50, 100, 200 ng/mL) and CXCL12β group (50, 100, 200 ng/mL). Transwell™ assay was used to observe the effect of CXCL12α and CXCL12β on migration of LX-2 cells. Expression of chemokine (C-X-C motif) receptor 4 (CXCR4) in LX-2 cells after treated with or without recombinant human CXCL12 was examined by Western blotting.


RESULTS
The number of migration cells in each group were as follows, control group (66.33 ± 11.43), PDGF-BB group (127.47 ± 31.68), 50, 100, 200 ng/mL CXCL12α group (106.13 ± 12.94, 125.87 ± 17.00, 137.07 ± 21.03), 50, 100, 200 ng/mL CXCL12β group (103.80 ± 11.26, 122.33 ± 19.46, 124.40 ± 15.16). Both CXCL12α and CXCL12β induced the migration of LX-2 cells, and migratory response increased in a dose-dependent manner. Western blotting demonstrated that CXCR4 was expressed in LX-2 cells and expression level of CXCR4 protein was not altered after stimulation with exogenous CXCL12.


CONCLUSIONS
Both CXCL12α and CXCL12β could induce the migration of HSCs.
Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
Xingxing
X
Yuan
Department of Etiology and Carcinogenesis, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China.
Ting
T
Xiao
Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
Fangfang
F
Dai
Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
Yanhua
Y
Yu
Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
Jinli
J
Lou
D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism
D054377:Chemokine CXCL12; D019718:Receptors, CXCR4