In vitro antibacterial activity of selected medicinal plants from lower Himalayas.
The present studies cover antibacterial activity of the crude methanolic extracts of 11 medicinal plants viz. Adhatoda vasica, Bauhenia variegate, Bombax ceiba, Carrisa opaca, Caryopteris grata, Debregeasia salicifolia, Lantana camara, Melia azedarach, Phyllanthus emblica, Pinus roxburghii and Olea ferruginea collected from lower Himalayas against two Gram positive (Staphylococcus aureus, Micrococcus luteus) and two Gram negative (Escherichia coli, Pseudomonas aureginosa) bacterial strains. The extracts were applied at four different concentrations (120 mg/mL, 90mg/mL, 60mg/mL and 30mg/mL) in dimethyl sulfoxide (DMSO) by using agar well diffusion method. Antibacterial activities against Staphylococcus aureus and Micrococcus luteus were observed formethanolic extracts of all the above mentioned plants. Greater antibacterial activity against Pseudomonas aeruginosa was only exhibited by Phyllanthus emblica, Pinus roxburghii, Debregeasia salicifolia and Lantana camara. Escherichia coli was highly resistant to all the plant extracts at all concentrations. It is inferred that methanolic crude extracts of the above mentioned plantsexhibitantibacterial activities against pathogenic bacteria, which proved the ethnobotanical importance of the selected plants that indigenous people use for cure against various diseases.
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Zulqarnain
Interdisciplinary Research Centre in Biomedical Materials,COMSATS Institute of Information Technology, Lahore, Pakistan.
Abdur
A
Rahim
Department of Environmental Sciences COMSATS, Abbottabad, Pakistan.
Khalid
K
Ahmad
Department of Botany University of Science and Technology Bannu, KPK, Pakistan.
Faizan
F
Ullah
Institute of Chemical Sciences, Gomal University, D. I. Khan, Pakistan.
Hamid
H
Ullah
Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Umar
U
Nishan
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
D000900:Anti-Bacterial Agents; D010936:Plant Extracts