BH3 Profiling Reveals Selectivity by Herpesviruses for Specific Bcl-2 Proteins To Mediate Survival of Latently Infected Cells.
Herpesviruses, including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), and Kaposi's sarcoma-associated herpesvirus, establish latency by modulating or mimicking antiapoptotic Bcl-2 proteins to promote survival of carrier cells. BH3 profiling, which assesses the contribution of Bcl-2 proteins towards cellular survival, was able to globally determine the level of dependence on individual cellular and viral Bcl-2 proteins within latently infected cells. Moreover, BH3 profiling predicted the sensitivity of infected cells to small-molecule inhibitors of Bcl-2 proteins.
Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, New York, USA.
Olesea
O
Cojohari
Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, New York, USA.
Christine M
CM
Burrer
Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, New York, USA.
Megan A
MA
Peppenelli
Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA.
Fardokht A
FA
Abulwerdi
Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA.
Zaneta
Z
Nikolovska-Coleska
Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, New York, USA chang@upstate.edu.
Gary C
http://orcid.org/0000-0001-8209-9777
GC
Chan
D000595:Amino Acid Sequence; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D003587:Cytomegalovirus / Q000472:pathogenicity / Q000502:physiology; D006564:Herpesviridae / Q000472:pathogenicity* / Q000502:physiology*; D006566:Herpesviridae Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000472:pathogenicity / Q000502:physiology; D019288:Herpesvirus 8, Human / Q000472:pathogenicity / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D008969:Molecular Sequence Data; D040081:Protein Array Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D017735:Virus Latency
D019253:Proto-Oncogene Proteins c-bcl-2; D014764:Viral Proteins