Identification of new SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat under water stressed condition.
Segregating F4 families from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes were made to identify SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (Triticum aestivum L.) under water-stressed condition and to map quantitative trait locus (QTL) for the three physiological traits. The parents and 150 F4 families were evaluated phenotypically for drought tolerance using two irrigation treatments (2500 and 7500 m3/ha). Using 400 SSR primers tested for polymorphism in testing parental and F4 families genotypes, the results revealed that QTL for leaf chlorophyll content, flag leaf senescence and cell membrane stability traits were associated with 12, 5 and 12 SSR markers, respectively and explained phenotypic variation ranged from 6 to 42%. The SSR markers for physiological traits had genetic distances ranged from 12.5 to 25.5 cM. These SSR markers can be further used in breeding programs for drought tolerance in wheat.
King Saud University Plant Production Department, College of Food and Agriculture Sciences Riyadh Saudi Arabia University of Alexandria Biotechnology Laboratory, Crop Science Department, Faculty of Agriculture Alexandria Egypt.
Mohamed N
MN
Barakat
King Saud University Botany and Microbiology Department, College of Pure Science Riyadh Saudi Arabia.
Mohamed
M
Saleh
King Saud University Plant Production Department, College of Food and Agriculture Sciences Riyadh Saudi Arabia.
Abdullah A
AA
Al-Doss
King Saud University Plant Production Department, College of Food and Agriculture Sciences Riyadh Saudi Arabia.
Khaled A
KA
Moustafa
King Saud University Plant Production Department, College of Food and Agriculture Sciences Riyadh Saudi Arabia National Research Centre Genetics and Cytology Department, Genetic Engineering and Biotechnology Division El-Dokki, Cairo Egypt.
Adel A
AA
Elshafei
King Saud University Botany and Microbiology Department, College of Pure Science Riyadh Saudi Arabia.
Fahed H
FH
Al-Qurainy
D002462:Cell Membrane / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D005819:Genetic Markers; D018515:Plant Leaves / Q000378:metabolism*; D040641:Quantitative Trait Loci; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics / Q000378:metabolism*; D014867:Water
D005819:Genetic Markers; D014867:Water; D002734:Chlorophyll
Physiological traits; QTL; SSR markers; Triticum aestivum