Paracellular pathway remodeling enhances sodium secretion by teleost fish in hypersaline environments.
In vertebrate salt-secreting epithelia, Na(+) moves passively down an electrochemical gradient via a paracellular pathway. We assessed how this pathway is modified to allow Na(+) secretion in hypersaline environments. Mummichogs (Fundulus heteroclitus) acclimated to hypersaline [2× seawater (2SW), 64‰] for 30 days developed invasive projections of accessory cells with an increased area of tight junctions, detected by punctate distribution of CFTR (cystic fibrosis transmembrane conductance regulator) immunofluorescence and transmission electron miscroscopy of the opercular epithelia, which form a gill-like tissue rich in ionocytes. Distribution of CFTR was not explained by membrane raft organization, because chlorpromazine (50 μmol l(-1)) and filipin (1.5 μmol l(-1)) did not affect opercular epithelia electrophysiology. Isolated opercular epithelia bathed in SW on the mucosal side had a transepithelial potential (Vt) of +40.1±0.9 mV (N=24), sufficient for passive Na(+) secretion (Nernst equilibrium voltage≡ENa=+24.11 mV). Opercular epithelia from fish acclimated to 2SW and bathed in 2SW had higher Vt of +45.1±1.2 mV (N=24), sufficient for passive Na(+) secretion (ENa=+40.74 mV), but with diminished net driving force. Bumetanide block of Cl(-) secretion reduced Vt by 45% and 29% in SW and 2SW, respectively, a decrease in the driving force for Na(+) extrusion. Estimates of shunt conductance from epithelial conductance (Gt) versus short-circuit current (Isc) plots (extrapolation to zero Isc) suggested a reduction in total epithelial shunt conductance in 2SW-acclimated fish. In contrast, the morphological elaboration of tight junctions, leading to an increase in accessory-cell-ionocyte contact points, suggests an increase in local paracellular conductance, compensating for the diminished net driving force for Na(+) and allowing salt secretion, even in extreme salinities.
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
Regina R F
RR
Cozzi
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
George N
GN
Robertson
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
Melanie
M
Spieker
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
Lauren N
LN
Claus
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
Gabriella M M
GM
Zaparilla
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5.
Kelly L
KL
Garrow
Department of Biology, St Francis Xavier University, J. Bruce Brown Hall, Room 214, 2320 Notre Dame Avenue, Antigonish, Nova Scotia, Canada B2G 2W5 bmarshal@stfx.ca.
William S
WS
Marshall
D000064:Acclimatization; D000818:Animals; D001693:Biological Transport, Active; D002746:Chlorpromazine / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005372:Filipin / Q000494:pharmacology; D023781:Fundulidae / Q000378:metabolism*; D008564:Membrane Potentials; D054712:Salinity; D012964:Sodium / Q000378:metabolism*
D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005372:Filipin; D012964:Sodium; D002746:Chlorpromazine
CFTR; Chloride cell; Electrophysiology; Euryhaline; Fundulus heteroclitus; Gill epithelium; Immunocytochemistry; Ionocyte; Teleost fish; Tight junction