The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells.
The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.
Inserm U1085 IRSET, Université de Rennes 1, 35042 Rennes, France.
Igor
I
Stuparevic
Inserm U1085 IRSET, Université de Rennes 1, 35042 Rennes, France.
Emmanuelle
E
Becker
Rowan University, School of Osteopathic Medicine, Stratford, NJ 08084, USA.
Michael J
MJ
Law
Inserm U1085 IRSET, Université de Rennes 1, 35042 Rennes, France. Electronic address: michael.primig@inserm.fr.
Michael
M
Primig
D020121:5' Untranslated Regions / Q000235:genetics; D000085:Acetates / Q000494:pharmacology*; D001483:Base Sequence; D004790:Enzyme Induction / Q000187:drug effects; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D006655:Histone Deacetylases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D008540:Meiosis; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000096:biosynthesis* / Q000235:genetics
D020121:5' Untranslated Regions; D000085:Acetates; D007527:Isoenzymes; C087335:PEP1 protein, S cerevisiae; D022661:RNA, Untranslated; D012097:Repressor Proteins; C071731:SIN3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C089979:UME6 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C071665:RPD3 protein, S cerevisiae; D006655:Histone Deacetylases
5′-UTR; Isoform; RPD3; UME6; VTH1; VTH2