The tomato I-3 gene: a novel gene for resistance to Fusarium wilt disease.
Plant resistance proteins provide race-specific immunity through the recognition of pathogen effectors. The resistance genes I, I-2 and I-3 have been incorporated into cultivated tomato (Solanum lycopersicum) from wild tomato species to confer resistance against Fusarium oxysporum f. sp. lycopersici (Fol) races 1, 2 and 3, respectively. Although the Fol effectors corresponding to these resistance genes have all been identified, only the I-2 resistance gene has been isolated from tomato. To isolate the I-3 resistance gene, we employed a map-based cloning approach and used transgenic complementation to test candidate genes for resistance to Fol race 3. Here, we describe the fine mapping and sequencing of genes at the I-3 locus, which revealed a family of S-receptor-like kinase (SRLK) genes. Transgenic tomato lines were generated with three of these SRLK genes and one was found to confer Avr3-dependent resistance to Fol race 3, confirming it to be I-3. The finding that I-3 encodes an SRLK reveals a new pathway for Fol resistance and a new class of resistance genes, of which Pi-d2 from rice is also a member. The identification of I-3 also allows the investigation of the complex effector-resistance protein interaction involving Avr1-mediated suppression of I-2- and I-3-dependent resistance in tomato.
Division of Plant Sciences, Research School of Biology, The Australian National University, Acton, ACT, 2601, Australia.
Ann-Maree
AM
Catanzariti
Division of Plant Sciences, Research School of Biology, The Australian National University, Acton, ACT, 2601, Australia.
Ginny T T
GTT
Lim
Division of Plant Sciences, Research School of Biology, The Australian National University, Acton, ACT, 2601, Australia.
David A
DA
Jones
D060054:Agrobacterium / Q000502:physiology; D000595:Amino Acid Sequence; D020029:Base Pairing; D016923:Cell Death; D002462:Cell Membrane / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium / Q000502:physiology*; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056726:Genetic Association Studies; D056426:Genetic Loci; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D016415:Sequence Alignment; D014026:Nicotiana / Q000166:cytology
D010940:Plant Proteins; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
Fusarium wilt; S-receptor-like kinase (SRLK); Solanum lycopersicum (tomato); effector-triggered immunity (ETI); fungal pathogen; plant resistance genes