Complex formation of blueberry (Vaccinium angustifolium) anthocyanins during freeze-drying and its influence on their biological activity.
Biological activity of polyphenols is influenced by their uptake and is highly influenced by their interactions with the food matrix. This study evaluated the complex formation of blueberry polyphenols with fruit matrixes such as pectin and cellulose and their effect on the biological and antiproliferative properties of human colon cell lines HT-29 and CRL 1790. Free or complexed polyphenols were isolated by dialyzing aqueous or methanolic blueberry homogenates. Seven phenolic compounds and thirteen anthocyanins were identified in blueberry extracts. Blueberry extracts showed varying degrees of antioxidant and antiproliferative activities, as well as α-glucosidase activity. Fruit matrix containing cellulose and pectin, or purified polygalacturonic acid and cellulose, did not retain polyphenols and showed very low antioxidant or antiproliferative activities. These findings suggest that interactions between polyphenols and the food matrix may be more complex than a simple association and may play an important role in the bioefficacy of blueberry polyphenols.
#Plant-Food Molecular Breeding, NutriOmics Group, Instituto Technológico de Estudios Superiores, Monterrey, N.L., Mexico.
Julieta
J
Correa-Betanzo
Priya
P
Padmanabhan
Milena
M
Corredig
Jayasankar
J
Subramanian
Gopinadhan
G
Paliyath
D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D029798:Blueberry Plants / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005519:Food Preservation; D005612:Freeze Drying; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology
D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
anthocyanins; antioxidant activity; antiproliferative activity; flavonoids; food matrix interactions; α-glucosidase