Application of paper spray-MS in PK studies using sunitinib and benzethonium as model compounds.
OBJECTIVE
Current bioanalytical methods applied in nonclinical PK studies for screening drug candidates demand significant amount of time and resources, hence, the need to develop alternative methods.


RESULTS
A proof-of-concept paper spray-MS method for the detection and quantitation of small molecules in plasma has been developed and validated using sunitinib and benzethonium as model compounds. The method includes single oral or intravenous administration of sunitinib to mice and serial micro-volume (20 µl) blood collection at different time intervals. The method is rapid with overall analysis time of 1 min and a full PK profile of sunitinib was obtained from a single mouse.


CONCLUSIONS
The paper spray-MS approach is simple, sensitive and can potentially enable significant reduction of animal use and cost.
School of Biological Sciences, Nanyang Technological University, Singapore.
John
J
Takyi-Williams
Xueming
X
Dong
Haiqing
H
Gong
Yang
Y
Wang
Wenying
W
Jian
Chuan-Fa
CF
Liu
Kai
K
Tang
D000818:Animals; D001558:Benzethonium / Q000032:analysis* / Q000493:pharmacokinetics*; D001774:Blood Chemical Analysis / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR; D010209:Paper; D011758:Pyrroles / Q000097:blood* / Q000493:pharmacokinetics*; D000077210:Sunitinib
D007211:Indoles; D011758:Pyrroles; D001558:Benzethonium; D000077210:Sunitinib