Proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus).
The Chinese giant salamander (Andrias davidianus), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin has developed mucous gland which could secrete a large amount of mucus under the scraping and electric stimulation, and the molting is the degraded skin stratum corneum. Although several proteomic studies have focused on functional proteomes of mammalian and frog skin, the skin proteome of Chinese giant salamander has not yet been carefully studied. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. Our findings indicated that 249 proteins were identified in the skin, 155 proteins in the mucus, and 97 proteins in the molting. Furthermore, Gene Ontology (GO) analysis showed that these proteins participated in various physiological activities, including extracellular matrix organization, defense, immune response, wound healing, respiration, etc. In conclusion, the proteomic results provide new insight in the aspects of the proteomes in the skin, mucus and the molting of Chinese giant salamander.


CONCLUSIONS
This was the first study to examine the protein expression abundance in the skin, mucus and molting of Chinese giant salamander by a proteomics approach. Meantime, the identification of a more global proteome in normal skin may provide a basis for characterizing and comparing the skin proteomes from other amphibian species.
State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China; Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Xiaofang
X
Geng
Department of Laboratory Animal Science, Third Military Medical University, Chongqing 400038, China.
Hong
H
Wei
Chongqing Kui Xu Biotechnology Incorporated Company, Kaixian Country, Chongqing 405423, China.
Haitao
H
Shang
Chongqing Kui Xu Biotechnology Incorporated Company, Kaixian Country, Chongqing 405423, China.
Minghui
M
Zhou
Chongqing Kui Xu Biotechnology Incorporated Company, Kaixian Country, Chongqing 405423, China.
Bing
B
Chen
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Fuchun
F
Zhang
State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China; Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China.
Xiayan
X
Zang
State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China; Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China.
Pengfei
P
Li
State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China; Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China.
Jingyan
J
Sun
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China; Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Jing
J
Che
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China; Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Yaping
Y
Zhang
State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, China; Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang 453007, China. Electronic address: cellkeylab@126.com.
Cunshuan
C
Xu
D029845:Amphibian Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012454:Salamandra / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
D029845:Amphibian Proteins; D020543:Proteome
Chinese giant salamander; Defense; Molting; Mucus; Proteome; Skin