In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety.
The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples. Hence, the purpose of this study was to utilize the insertion sequence IS6110 for amplification of DNA from a smear-positive sample for tuberculosis (TB) diagnosis. Among the 52 positive bacilloscopies, sensitivity, specificity, positive predictive value and negative predictive value were 52.3%, 100%, 100% and 89.7%, respectively whereas accuracy was 90.7%. The IS6110-based PCR for TB diagnosis developed in DNA extracted from a positive smear is a fast, simple, specific, and safe method.
Universidade Federal de Minas Gerais, UFMG, Brasil.
Isabela Neves
IN
de Almeida
Clinical Hospital of UFMG, Brasil.
Agdemir Valéria
AV
Aleixo
Universidade Federal de Minas Gerais, UFMG, Brasil.
Wânia da Silva
Wda S
Carvalho
Research Group Coordinator Faculdade de Medicina/UFMG_REDE-TB, Brasil. Electronic address: silvanaspindola@gmail.com.
Silvana Spindola
SS
de Miranda
D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012449:Safety; D013048:Specimen Handling; D014376:Tuberculosis / Q000175:diagnosis*
D004269:DNA, Bacterial
Biosafety; Bioseguridad; IS6110-PCR; Láminas; M. tuberculosis; PCR IS6110; Slides; Tuberculosis