DNA sequence polymorphism within the bovine adenosine monophosphate deaminase 1 (AMPD1) is associated with production traits in Chinese cattle.
The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. The five 19-bp polymerase chain reaction products of the AMPD1 gene exhibited 3 genotypes and 2 alleles: WW: homozygote genotype (wild-type); DD: homozygote genotype (mutant-type); WD: heterozygote genotype. Frequencies of the W allele varied from 66.15-70.35%. The associations between the 18-bp deletion mutation in the AMPD1 gene with production traits in 226 Jia-Xian red cattle was analyzed. The animals with genotype WW showed significantly higher heart girth and body weight than those with genotypes WD and DD at 24 months (P < 0.01). Our results indicate that the deletion mutation in the AMPD1 gene is associated with production traits, and may be used for marker-assisted selection in beef cattle breeding programs.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
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Wei
Research Center of Cattle Engineering Technology in Henan, Zhengzhou, Henan, China.
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Wang
Institute of Animal Husbandry and Veterinary Medicine, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
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Chen
Research Center of Cattle Engineering Technology in Henan, Zhengzhou, Henan, China.
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Niu
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China kuili@iascaas.net.cn.
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Li
D000659:AMP Deaminase / Q000235:genetics*; D000818:Animals; D001835:Body Weight / Q000235:genetics*; D001947:Breeding; D002417:Cattle; D056726:Genetic Association Studies; D005838:Genotype; D008460:Meat; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics
D000659:AMP Deaminase