Simultaneous quantification of digoxin, digitoxin, and their metabolites in serum using high performance liquid chromatography-tandem mass spectrometry.
The study describes an LC-MS/MS method for simultaneous quantification of the cardiac glycosidic drugs digoxin and digitoxin and several of their metabolites. The assay represents a useful reference method for immunoassay-based tests, which are easily biased by the presence of metabolites of the target analytes or structurally similar substances.
Roche Diagnostics GmbH, Penzberg, Germany.|Institute of Bioanalytical Chemistry, Saarland University, Saarbrücken, Germany.
Caroline
C
Bylda
Roche Diagnostics GmbH, Penzberg, Germany.
Roland
R
Thiele
Roche Diagnostics GmbH, Penzberg, Germany.
Uwe
U
Kobold
Institute of Bioanalytical Chemistry, Saarland University, Saarbrücken, Germany.
Dietrich A
DA
Volmer
D000889:Anti-Arrhythmia Agents / Q000097:blood / Q000378:metabolism; D002316:Cardiotonic Agents / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004074:Digitoxin / Q000097:blood* / Q000378:metabolism; D004077:Digoxin / Q000097:blood* / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000889:Anti-Arrhythmia Agents; D002316:Cardiotonic Agents; D004077:Digoxin; D004074:Digitoxin
LC-MS/MS; digitoxin; digoxin; sample preparation