Simultaneous determination of camptothecin and 10-hydroxycamptothecine in the Camptotheca acuminate, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection.
Camptotheca acuminata Decne is an important medicinal plant that contains various cytotoxic alkaloids, such as camptothecine (CPT) and 10-hydroxycamptothecine (HCPT). A rapid and sensitive liquid chromatography with fluorescence detection (LC-FLD) method for the quantification of CPT and HCPT is described. The separation was carried out on a DL-Cl8 column (4.6 × 150 mm, 5 µm), with the mobile phase of acetonitrile-sodium dihydrogen phosphate buffer (10 mm) using an gradient elution at the flow rate of 0.6 mL/min. The LC-FLD method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the above components. The lower detection limits of CPT and HCPT were 0.4 and 0.1 ng/mL, respectively. The precision was <1.58% and the mean recovery of the analytes was 96.0-98.6%. The LC-FLD method was successfully applied to determine CPT and HCPT in real samples including C. acuminate, HCPT injection and rat plasma.
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.|Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, 442008, China.
Peng
P
Li
Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, 442008, China.
Qinhua
Q
Chen
Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, 442008, China.
Fei
F
Yu
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.
Xiaoyu
X
Xie
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.|Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, 442008, China.
Sicen
S
Wang
D000284:Administration, Oral; D000818:Animals; D029769:Camptotheca / Q000737:chemistry*; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D057230:Limit of Detection; D010946:Plants, Medicinal / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; C028098:10-hydroxycamptothecin; D002166:Camptothecin
10-hydroxycamptothecine; Camptotheca acuminata Decne; camptothecin; liquid chromatography with fluorescence detection; plasma