Evaluation of the immunochromatographic device for the detection of verotoxins in cultures of food materials.
The immunochromatographic assay, which targets Shiga toxin 1/verotoxin 1 (VT1) and/or Shiga toxin 2/verotoxin 2 (VT2) independently with same test device, was used for easily, rapidly and specifically detecting verotoxin-producing Escherichia coli among E. coli strains from food and fecal materials. All 10 strains of VT 1 and/or VT 2- producing E. coli among E. coli isolates from various sources showed a positive reaction to VT1- or VT2- antibodies, but other gram-negative and positive bacterial species had a negative reaction. Bacterial counts of 10(8) cfu/ml in enrichment broth and food suspension were required for the detection of VT-producing E. coli. The IC assay described here could detect easily and specifically the verotoxin-producing E. coli within 20 min by pure culture.
Laboratory of Hygiene, Kagawa Nutrition University.
Shigeko
S
Ueda
Miki
M
Iwase
Manami
M
Yamaguchi
D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D012680:Sensitivity and Specificity; D022603:Shiga Toxins / Q000032:analysis*; D013997:Time Factors
D022603:Shiga Toxins