Urodynamic changes in mice with experimental autoimmune encephalomyelitis correlate with neurological impairment.
OBJECTIVE
Neurogenic bladder dysfunction is a major issue in Multiple Sclerosis (MS). High intravesical pressure should be treated early. Available therapies are insufficient and there is need for drug development and investigation of pathogenesis. Experimental Autoimmune Encephalomyelitis (EAE) in rodents is a well validated model to study MS. Previous research has shown that these animals develop urinary symptoms. However, from clinical studies, we know that symptoms do not necessarily reflect changes in bladder pressure. This paper aims to provide a complete overview of urodynamic changes in a model for detrusor overactivity in MS.


METHODS
Female C57Bl/6J mice, injected with MOG35-55 and control mice, injected with vehicle (Complete Freund's adjuvant), were monitored daily for neurologic symptoms. Within 1 month after symptom development, mice were used for cystometry or histology of the bladder.


RESULTS
Increasing disease score correlated with increased micturition frequency, basal pressure, and average pressure, and with a decrease in functional bladder capacity, voiding amplitude, and maximum pressure.


CONCLUSIONS
This paper provides a detailed description of bladder function in C57Bl/6J mice with Myelin Oligodendrocyte Glycoprotein peptide (MOG35-55 ) induced EAE. This EAE model induces detrusor overactivity in close relationship to neurological impairment. EAE in mice is a suitable model to study detrusor overactivity in MS. Neurourol. Urodynam. 35:450-456, 2016. © 2015 Wiley Periodicals, Inc.
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.|Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.
J
J
Franken
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.
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T
Gevaert
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.|Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.|Hasselt University - Biomedical Research Institute, Diepenbeek, Belgium.
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P
Uvin
Hasselt University - Biomedical Research Institute, Diepenbeek, Belgium.
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K
Wauterickx
Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.
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AC
Boeve
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.|Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.
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R
Rietjens
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.|Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.
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M
Boudes
Hasselt University - Biomedical Research Institute, Diepenbeek, Belgium.
J J A
JJ
Hendriks
Hasselt University - Biomedical Research Institute, Diepenbeek, Belgium.
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N
Hellings
Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Research Platform Leuven (TRPLe), KU Leuven - University of Leuven, Leuven, Belgium.
T
T
Voets
Department of Development and Regeneration, Laboratory of Experimental Urology, UZ Leuven - University of Leuven, Leuven, Belgium.
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D
De Ridder
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000503:physiopathology*; D005260:Female; D051379:Mice; D001743:Urinary Bladder / Q000503:physiopathology*; D001750:Urinary Bladder, Neurogenic / Q000503:physiopathology*; D053201:Urinary Bladder, Overactive / Q000503:physiopathology*; D014554:Urination; D014563:Urodynamics / Q000502:physiology*
animal; autoimmune; encephalomyelitis; experimental urinary bladder; neurogenic urinary bladder; overactive multiple sclerosis urodynamics models