Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: a metabolomic approach.
In order to highlight differences in the metabolic profile of healthy (control) compared with asphyxiated newborns, by using untargeted metabolomic approach coupled with (1)H NMR spectroscopy, we evaluated the effects of asphyxia on newborn urine metabolites. Our results showed that lactate, glucose and TMAO, together with threonine plus 3-hydroxyisovalerate are the metabolites more characterizing the asphyxiated group; lower contribute to discrimination is related to other metabolites such as dimethylglycine, dimethylamine, creatine, succinate, formate, urea and aconitate. After 24-48h from resuscitation preterm asphyctic neonates showed their recovery pattern that still can be differentiated by the controls.
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy. Electronic address: mc.malergue@orange.fr.
M
M
Longini
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
S
S
Giglio
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
S
S
Perrone
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
A
A
Vivi
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
M
M
Tassini
Neonatal Intensive Care Unit, Puericulture Institute and Neonatal Section, University of Cagliari, Italy.
V
V
Fanos
1st Department of Neonatology, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
K
K
Sarafidis
Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
G
G
Buonocore
D001238:Asphyxia Neonatorum / Q000378:metabolism / Q000652:urine*; D006801:Humans; D007231:Infant, Newborn; D055432:Metabolomics / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy
(1)H NMR; Asphyxia; Metabolomics; Urine