A single bout of dynamic exercise by healthy adults enhances the generation of monocyte-derived-dendritic cells.
The ex vivo generation of monocyte-derived-dendritic cells (mo-DCs) has facilitated the use of DCs in immunotherapy research. However, low blood monocyte numbers frequently limit the manufacture of sufficient numbers of mo-DCs for subsequent experimental and clinical procedures. Because exercise mobilizes monocytes to the blood, we tested if acute dynamic exercise by healthy adults would augment the generation of mo-DCs without compromising their differentiation or function. We compared mo-DC generation from before- and after-exercise blood over 8-days of culture. Function was assessed by FITC-dextran uptake and the stimulation of autologous cytomegalovirus (pp65)-specific-T-cells. Supporting the hypothesis, we found a near fourfold increase in number of mo-DCs generated after-exercise. Furthermore, relative FITC-dextran uptake, differentiation rate, and stimulation of pp65-specific-T-cells did not differ between before- and after-exercise mo-DCs. We conclude that exercise enhances the ex vivo generation of mo-DCs without compromising their function, and so may overcome some limitations associated with manufacturing these cells for immunotherapy.
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA.
Emily C P
EC
LaVoy
Program for Cell Enhancement and Technologies for Immunotherapy, Children's National Health System and The George Washington University, Washington D.C., USA.
Catherine M
CM
Bollard
Program for Cell Enhancement and Technologies for Immunotherapy, Children's National Health System and The George Washington University, Washington D.C., USA.
Patrick J
PJ
Hanley
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA.
Daniel P
DP
O'Connor
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA.
Thomas W
TW
Lowder
Department of Clinical Psychology, University of Amsterdam, Amsterdam, The Netherlands.
Jos A
JA
Bosch
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA. Electronic address: rjsimpso@central.uh.edu.
Richard J
RJ
Simpson
D000328:Adult; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000276:immunology / Q000493:pharmacokinetics; D015444:Exercise; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010750:Phosphoproteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology; D055815:Young Adult
D003911:Dextrans; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate
Dendritic cells; Immunology; Immunotherapy; Physical activity