Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells.
Dysregulation of androgen-binding protein (ABP) is associated with a number of endocrine and andrology diseases. However, the ABP metabolism in Sertoli cells is largely unknown. We report that autophagy degrades ABP in rat Sertoli cells, and the autophagic clearance of ABP is regulated by testosterone, which prolongs the ABP biological half-life by inhibiting autophagy. Further studies identified that the autophagic clearance of ABP might be selectively regulated by testosterone, independent of stress (hypoxia)-induced autophagic degradation. These data demonstrate that testosterone up-regulates ABP expression at least partially by suppressing the autophagic degradation. We report a novel finding with respect to the mechanisms by which ABP is cleared, and by which the process is regulated in Sertoli cells.
1] Department of Biobank, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 1, 1630 DongFang Road, Shanghai. 200127, China [2] Department of Urology, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 7, 1630 DongFang Road, Shanghai. 200127, China.
Yi
Y
Ma
Department of Central Laboratory, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 5, 1630 DongFang Road, Shanghai. 200127, China.
Hao-Zheng
HZ
Yang
Department of Central Laboratory, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 5, 1630 DongFang Road, Shanghai. 200127, China.
Long-Mei
LM
Xu
Department of Urology, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 7, 1630 DongFang Road, Shanghai. 200127, China.
Yi-Ran
YR
Huang
Department of Biobank, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 1, 1630 DongFang Road, Shanghai. 200127, China.
Hui-Li
HL
Dai
Department of Biobank, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Building 1, 1630 DongFang Road, Shanghai. 200127, China.
Xiao-Nan
XN
Kang
D000727:Androgen-Binding Protein / Q000378:metabolism*; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D002478:Cells, Cultured; D008297:Male; D008657:Metabolic Clearance Rate; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology* / Q000502:physiology*; D013739:Testosterone / Q000378:metabolism*
D000727:Androgen-Binding Protein; D013739:Testosterone