[Inhibitory effect of wogonin on the proliferation and invasion of glioblastoma U87 cells and related mechanism].
OBJECTIVE
To explore the effect of wogonin on the proliferation and invasion of glioblastoma U87 cells and its related mechanism.


METHODS
Glioblastoma U87 cells were cultured in vitro in RPMI1640 medium added with 100 mL/L fetal bovine serum. After cell adherence, the cells were inoculated with 20 μL culture solution (control group), 20 μL wogonin solutions (0, 50 and 100) μmol/L for 48 hours, respectively. The 0 μmol/L wogonin group used 20 μL PBS instead. Cell proliferation was analyzed by MTT assay. The cell invasion ability was detected using Transwell™ invasion assay. The expressions of ezrin mRNA was examined through real-time quantitative PCR. The expressions of ezrin, Bcl-2 and Bax proteins as well as phosphorylated ezrin protein level were measured by Western blotting. Apoptosis index (AI) was determined through terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay.


RESULTS
The inhibitory rate of cell proliferation significantly increased in 50 and 100 μmol/L wogonin groups as compared with 0 μmol/L wogonin group and control group. Moreover, that was higher in 100 μmol/L wogonin group than 50 μmol/L wogonin group. In comparison with control and 0 μmol/L wogonin groups, the mean cell numbers of permeated membrane, levels of ezrin mRNA, ezrin protein and Bcl-2 protein, and phosphorylated ezrin protein level gradually decreased but the level of Bax protein and AI were gradually elevated with the increase of wogonin concentrations; however, there was no significant difference in these indicators between 0 μmol/L wogonin group and control group.


CONCLUSIONS
Wogonin could attenuate the proliferation and invasion of wogonin U87 cells, which may be associated with the inhibition of ezrin protein expression and phosphorylation activity and the induction of cell apoptosis.
Department of Neurosurgery, First Affiliated Hospital, University of South China, Hengyang 421001, China.
Zhiqing
Z
Zeng
Department of Neurosurgery, First Affiliated Hospital, University of South China, Hengyang 421001, China.
Hong
H
Liu
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology*; D005909:Glioblastoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C085514:wogonin