Regulatory T-cell subsets in response to specific Mycobacterium tuberculosis antigens in vitro distinguish among individuals with different QTF and TST reactivity.
Regulatory T cells (Tregs), a subset of CD4+ T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with mycobacterial antigens ESAT-6, CFP-10, and TB7.7 to assess their capacity to distinguish subjects with different reactivity to the QuantiFERON-TB® Gold In-Tube (QFT-IT) test and the tuberculin skin test (TST). Increased frequencies of CD4+CD25highCD39+ cells were found for the [TST+, QTF+] compared with the [TST+, QTF-] group. Also, higher frequencies were observed for the [TST+, QTF+] compared with the [TST+, QTF-] and [TST-, QTF-] groups in CD4+CD25highFoxp3+ and CD4+CD25highCD39+Foxp3+ populations. Receiver operating characteristics (ROC curve) analysis confirmed these discriminating results. QFT-IT and TST quantitative values correlated with several Treg population frequencies.
Medical Research Unit Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
C J
CJ
Serrano
Medical Research Unit Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico; National Council of Science and Technology (CONACYT), Catedras-CONACYT, Mexico.
J E
JE
Castañeda-Delgado
Medical Research Unit Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
J L
JL
Trujillo-Ochoa
Department of Immunology, School of Medicine, Autonomous University of San Luis Potosi-UASLP, SLP, Mexico.
R
R
González-Amaro
Medical Research Unit Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
M H
MH
García-Hernández
Medical Research Unit Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico. Electronic address: enciso_2000@yahoo.com.
J A
JA
Enciso-Moreno
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D059425:Interferon-gamma Release Tests; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism; D055985:Latent Tuberculosis / Q000276:immunology*; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000635:transmission*; D055815:Young Adult
D000942:Antigens, Bacterial; D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit; C072681:Mycobacterium tuberculosis antigens; D001081:Apyrase; C067869:CD39 antigen
QFT; Regulatory T cells; TST; Tuberculosis