[Study on UPLC fingerprint of Xiasangju Granules].
OBJECTIVE
To establish a UPLC fingerprint method of Xiasangju Granules.


METHODS
UPLC analysis was performed on a Waters ACQUITY UPLC H-Class system and carried out at 30 °C on a Waters Column ACQUITY UPLC BEH C18 (2.1 mm x 50 mm, 1.7 μm). A binary gradient elution system was composed of acetonitrile (phase A)and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 320 nm,the mobile flow rate was at 0.4 mL/min. A matrix including 16 variations (characteristic peaks area)and 12 samples was constructed for similarity evaluation, cluster analysis and principle component analysis.


RESULTS
The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 16 characteristic peaks were designated. 12 samples were divided into 6 groups.


CONCLUSIONS
It is a reliable, available and quick method for quality control of Xiasangju Granules.
Bo-Hou
BH
Xia
Yi
Y
Cao
Wen-Jian
WJ
Xie
Zhao-Dong
ZD
Xu
Li-Mei
LM
Lin
Duan-Fang
DF
Liao
D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control
D004365:Drugs, Chinese Herbal