S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting.
Selective targeting of tumor cells by nanoparticle-based drug delivery systems is highly desirable because it maximizes the drug concentration at the desired target while simultaneously protecting the surrounding healthy tissues. Here, we show a design for smart nanocarriers based on a biomimetic approach that utilizes the building principle of virus envelope structures. Emulsomes and CurcuEmulsomes comprising a tripalmitin solid core surrounded by phospholipid layers are modified by S-layer proteins that self-assemble into a two-dimensional array to form a surface layer. One significant advantage of this nanoformulation is that it increases the solubility of the lipophilic anti-cancer agent curcumin in the CurcuEmulsomes by a factor of 2700. In order to make the emulsomes specific for IgG, the S-layer protein is fused with two protein G domains. This S-layer fusion protein preserves its recrystallization characteristics, forming an ordered surface layer (square lattice with 13 nm unit-by-unit distance). The GG domains are presented in a predicted orientation and exhibit a selective binding affinity for IgG.
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 11, 1190 Vienna, Austria; Department of Biomedical Engineering, School of Engineering and Natural Sciences, Istanbul Medipol University, Ekinciler Cad. No. 19 Kavacık Kavşağı, Beykoz 34810, Istanbul, Turkey. Electronic address: m.h.ucisik@gmail.com.
Mehmet H
MH
Ucisik
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 11, 1190 Vienna, Austria.
Seta
S
Küpcü
Institute for Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 11, 1190 Vienna, Austria.
Andreas
A
Breitwieser
Octapharma GmbH, Oberlaaer Straße 235, 1100 Vienna, Austria.
Nicola
N
Gelbmann
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 11, 1190 Vienna, Austria.
Bernhard
B
Schuster
Institute for Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 11, 1190 Vienna, Austria.
Uwe B
UB
Sleytr
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D001406:Bacillaceae / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004655:Emulsions; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018796:Immunoconjugates / Q000737:chemistry / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019251:Nucleocapsid / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D014280:Triglycerides / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D001426:Bacterial Proteins; D004655:Emulsions; D018796:Immunoconjugates; D007074:Immunoglobulin G; D008081:Liposomes; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; C120455:SbpA protein, bacteria; D014280:Triglycerides; C005859:tripalmitin; D003474:Curcumin
Active drug delivery; Curcumin; Emulsomes; Immunoglobulin G (IgG) Targeting; S-layer (fusion) proteins