BSA as additive: A simple strategy for practical applications of PNA in bioanalysis.
Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.
Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea; Center for RNA Research, Institute for Basic Science, Seoul National University, Seoul 151-747, Republic of Korea.
Jieon
J
Lee
Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea; Center for RNA Research, Institute for Basic Science, Seoul National University, Seoul 151-747, Republic of Korea.
Il-Soo
IS
Park
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
Henna
H
Kim
Center for RNA Research, Institute for Basic Science, Seoul National University, Seoul 151-747, Republic of Korea.
Jae-Sung
JS
Woo
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
Byong-Seok
BS
Choi
Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea; Center for RNA Research, Institute for Basic Science, Seoul National University, Seoul 151-747, Republic of Korea; Lemonex Inc., Seoul 151-742, Republic of Korea. Electronic address: dalheemin@snu.ac.kr.
Dal-Hee
DH
Min
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation*; D010087:Oxides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D010087:Oxides; D020135:Peptide Nucleic Acids; D012710:Serum Albumin, Bovine; D006108:Graphite; D004247:DNA
Bioanalysis; Bovine serum albumin; Graphene oxide; Peptide nucleic acid; Signal enhancement