Automated centrifugal-microfluidic platform for DNA purification using laser burst valve and coriolis effect.
We report a fully automated DNA purification platform with a micropored membrane in the channel utilizing centrifugal microfluidics on a lab-on-a-disc (LOD). The microfluidic flow in the LOD, into which the reagents are injected for DNA purification, is controlled by a single motor and laser burst valve. The sample and reagents pass successively through the micropored membrane in the channel when each laser burst valve is opened. The Coriolis effect is used by rotating the LOD bi-directionally to increase the purity of the DNA, thereby preventing the mixing of the waste and elution solutions. The total process from the lysed sample injection into the LOD to obtaining the purified DNA was finished within 7 min with only one manual step. The experimental result for Salmonella shows that the proposed microfluidic platform is comparable to the existing devices in terms of the purity and yield of DNA.
School of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do, 440-746, South Korea.
Min-Seong
MS
Choi
Jae-Chern
JC
Yoo
D002498:Centrifugation; D003312:Coriolis Force; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D056656:Lab-On-A-Chip Devices; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012475:Salmonella / Q000737:chemistry* / Q000235:genetics
D004247:DNA