Increased α1-adrenoreceptor activity is required to sustain blood pressure in female rats under food restriction.
OBJECTIVE
We evaluated the effect of food restriction (FR) on the various reflexes involved in short term cardiovascular regulation; we also evaluated the contribution of the sympathetic nervous systemand of the plasmatic nitric oxide (NO) in the development of the counterregulatory cardiovascular changes triggered by FR.


METHODS
Female rats were subjected to FR for 14 days, and after this period biochemical measurements of biochemical parameters were performed. For physiological tests, animals were anaesthetised, and a catheter was inserted into the femoral artery and vein for the acquisition of blood pressure and heart hate, and drug infusion, respectively.We then tested the Bezold–Jarisch reflex, the baroreflex and chemoreflex and the effect of the infusion of adrenergic receptor antagonists in control and food restricted animals.


RESULTS
The rats subjected to severe FR presented biochemical changes characteristic of malnutrition with a great catabolic state. FR also led to hypotension and bradycardia besides reducing the plasmatic concentration of NO. Moreover, activation of the Bezold–Jarisch reflex induced a more pronounced hypotensive response in animals subjected to FR. Intravenous infusion of a α1-adrenoreceptor antagonist induced a greater hypotensive response and a more pronounced tachycardic response in animals under food restriction,while the infusion of β-adrenoreceptor antagonist induced lower increases in blood pressure in these animals.


CONCLUSIONS
Our results suggest that an increased α1-adrenoreceptor activity in the resistance arteries coupled with a reduction of plasmatic NO contributes in a complementary manner to maintain the blood pressure levels in animals under FR.
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Aline Arlindo
AA
de Souza
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil. Electronic address: rodrigo.menezes@iceb.ufop.br.
Rodrigo Cunha
RC
de Menezes
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Aline Rezende
AR
Abreu
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Glaucy Rodrigues
GR
Araujo
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Daniela Caldeira
DC
Costa
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Deoclecio Alves
DA
Chianca
D000818:Animals; D000856:Anorexia Nervosa / Q000378:metabolism / Q000503:physiopathology; D017704:Baroreflex; D001794:Blood Pressure; D031204:Caloric Restriction; D005260:Female; D006339:Heart Rate; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*
D018340:Receptors, Adrenergic, alpha-1; D009569:Nitric Oxide
Cardiovascular reflexes; Food restriction; α1-Adrenoreceptors