Proteomic screening of antigenic proteins from the hard tick, Haemaphysalis longicornis (Acari: Ixodidae).
Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis.
Department of Veterinary Parasitology , College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University, Jeonju 561-756, Korea.
Young-Ha
YH
Kim
Department of Veterinary Parasitology , College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University, Jeonju 561-756, Korea ; Department of Medicine, Surgery and Obstetrics, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
Mohammad Saiful
MS
Slam
Department of Veterinary Parasitology , College of Veterinary Medicine and Biosafety Research Centre, Chonbuk National University, Jeonju 561-756, Korea.
Myung-Jo
MJ
You
D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000276:immunology*; D060829:Arthropod Proteins / Q000032:analysis* / Q000276:immunology*; D004586:Electrophoresis; D015151:Immunoblotting; D026863:Ixodidae / Q000737:chemistry*; D008403:Mass Screening; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000941:Antigens; D060829:Arthropod Proteins
Haemaphysalis longicornis; antigenic protein; proteomic approach; tick