Down-expression pattern of Ku70 and p53 coexisted in colorectal cancer.
To address the relationship of altered expression of double-strand break repair proteins Ku70 and p53 in clinical colorectal cancer (CRC), we examined the expression pattern of Ku70 and p53 by using fluorescent immunohistochemistry and real-time PCR assays in CRC and pericancerous samples from 152 Chinese patients. The results showed that down-expression pattern of both Ku70 and p53 coexisted in the CRC samples with significant correlating rate (R (2) = 0.9103; P < 0.001), and the down-expression of Ku70 and p53 was significantly associated with the advanced tumor node metastasis stage (Ku70: HR 3.453 in recurrence and 4.182 in survival, P < 0.001; P53: HR 3.114 in recurrence and 4.113 in survival, P < 0.001). The down-regulated Ku70 and p53 were associated with poor disease-free survival. Loss of Ku70 and p53 expression might serve as a biomarker of poor prognosis in CRC patients.
Department of Toxicology, School of Public Health, Guilin Medical University, North Huancheng 2nd Road, Guilin, 541004, Guangxi, China.
Yuanfang
Y
Lu
Jingyan
J
Gao
Yuanming
Y
Lu
D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D000072200:Ku Autoantigen; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen