The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response.
We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of Golden Syrian hamsters. None of the vaccines tested conferred protection against a challenge using a virulent Leptospira interrogans strain. These results demonstrated that nanotubes can be used as antigen carriers for delivery in hosts and the induction of a humoral immune response against purified leptospiral antigens used in subunit vaccine preparations.
Centro de Desenvolvimento Tecnológico.
Daiane D
DD
Hartwig
Centro de Desenvolvimento Tecnológico.
Kátia L
KL
Bacelo
Centro de Desenvolvimento Tecnológico.
Thaís L
TL
Oliveira
Centro de Desenvolvimento Tecnológico.
Rodrigo
R
Schuch
Centro de Desenvolvimento Tecnológico.
Fabiana K
FK
Seixas
Centro de Desenvolvimento Tecnológico.
Tiago
T
Collares
Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Oscar
O
Rodrigues
Centro de Desenvolvimento Tecnológico.
Cláudia P
CP
Hartleben
Centro de Desenvolvimento Tecnológico.
Odir A
OA
Dellagostin
D000538:Aluminum Silicates / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002245:Carbon Dioxide / Q000276:immunology; D000077215:Clay; D006224:Cricetinae; D005240:Feasibility Studies; D056724:Immunity, Humoral / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D008647:Mesocricetus; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology
D000538:Aluminum Silicates; D000941:Antigens; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins; D002245:Carbon Dioxide; C041069:carboxyl radical; D000077215:Clay