A novel marker for undifferentiated human embryonic stem cells.
Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs. The antigen recognized by MAb2 is expressed on the cell surface of undifferentiated hESCs; three diffused bands with molecular mass between 30 and 60 kDa in the lysates of hESCs were diminished during hESC differentiation into neural cells. The expression of MAb2 antigen was also observed on the plasma membrane of lung cancer cells, and MAb2 detected 55, 50, and 35 kDa protein bands in the cell lysates. Immunoprecipitation followed by proteomics analyses identified CD147/basigin as a MAb2 antigen. Finally, the positive expression of CD147/basigin protein in undifferentiated hESCs was confirmed. These results suggested that CD147/basigin could be another undifferentiated hESC marker.
1 Environmental Health Science Laboratory, Sumitomo Chemical Co. , Osaka, Japan .
Kiyoshi
K
Higashi
Masaki
M
Yagi
Tatsuhiko
T
Arakawa
Kouji
K
Asano
Kumiko
K
Kobayashi
Taro
T
Tachibana
Koichi
K
Saito
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D051926:Basigin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; C497886:BSG protein, human; D015415:Biomarkers; D051926:Basigin