C-type lectin-like domain and fibronectin-like type II domain of phospholipase A(2) receptor 1 modulate binding and migratory responses to collagen.
Phospholipase A2 receptor 1 (PLA2R) mediates collagen-dependent migration. The mechanisms by which PLA2R interacts with collagen remain unclear. We produced HEK293 cells expressing full-length wild-type PLA2R or a truncated PLA2R that lacks fibronectin-like type II (FNII) domains or several regions of C-type lectin-like domain (CTLD). We show that the CTLD1-2 as well as the FNII domain of PLA2R are responsible for binding to collagen and for collagen-dependent migration. Thus, multiple regions and domains of the extracellular portion of PLA2R participate in the responses to collagen. These data suggest a potentially new mechanism for PLA2R-mediated biological response beyond that of a receptor for secretory PLA2.
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Soichiro
S
Takahashi
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Kazuhiro
K
Watanabe
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Yosuke
Y
Watanabe
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Daisuke
D
Fujioka
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Takamitsu
T
Nakamura
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Kazuto
K
Nakamura
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan.
Jun-ei
JE
Obata
Department of Internal Medicine II, University of Yamanashi, Faculty of Medicine, Chuo, Yamanashi, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan. Electronic address: kugiyama@yamanashi.ac.jp.
Kiyotaka
K
Kugiyama
D000818:Animals; D001665:Binding Sites; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D051379:Mice; D009154:Mutation; D017434:Protein Structure, Tertiary; D054507:Receptors, Phospholipase A2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D005353:Fibronectins; D037181:Lectins, C-Type; C516360:PLA2R1 protein, human; C516357:Pla2r1 protein, mouse; D054507:Receptors, Phospholipase A2; D003094:Collagen
Binding; Collagen; Fibronectin-like type II domain; Migration; Phospholipase A(2) receptor 1; Secretory phospholipase A(2)