Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice.
Brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus. The Outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. In the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the Truncated form of Omp31 (TOmp31) and L7-L12 antigens. Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against B. melitensis and B. abortus challenge. Moreover, rL7/L12-TOmp31 elicited a strong specific IgG response (higher IgG2a titers) and significant IFN-γ/IL2 production and T-cell proliferation was also observed. The T helper1 (Th1) oriented response persisted for 12 weeks after the first immunization. The rL7/L12-TOmp31 could be a new potential antigen candidate for the development of a subunit vaccine against B. melitensis and B. abortus.
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Maryam
M
Golshani
Department of Molecular Immunology and Vaccine Research, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Sima
S
Rafati
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Amir
A
Dashti
Department of Molecular Immunology and Vaccine Research, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Elham
E
Gholami
Department of Bacteriology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Seyed Davar
SD
Siadat
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Mana
M
Oloomi
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Anis
A
Jafari
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran. Electronic address: bouzari@pasteur.ac.ir.
Saeid
S
Bouzari
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D002002:Brucella / Q000235:genetics / Q000276:immunology*; D002004:Brucella Vaccine / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D002006:Brucellosis / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014611:Vaccination
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D018664:Interleukin-12; D007371:Interferon-gamma
Brucella abortus; Brucella melitensis; Fusion protein; Human vaccine; L7/L12; Truncated Omp31