Role of extracellular polymeric substances in biosorption of dye wastewater using aerobic granular sludge.
In this study, the role of extracellular polymeric substances (EPS) in biosorption of dye wastewater was evaluated using aerobic granular sludge as biosorbent. Based on the experimental data, the removal efficiencies of methylene blue (MB) by EPS and Sludge were 9.38 and 80.72%, respectively, implying that EPS made a certain contribution for MB removal. The adsorption rates of EPS, Sludge, and total Sludge+EPS for MB were better fitted with pseudo-second order kinetic model, and the equilibrium adsorption isotherm data agreed well with Langmuir model. The interaction between EPS and MB was explored by a combined three-dimensional excitation-emission matrix (3D-EEM) and synchronous fluorescence spectra. 3D-EEM indicated that protein- and humic acid-like substances were the main peaks of EPS, and gradually quenched with increased MB concentrations. According to synchronous fluorescence spectra, the main fluorescence quenching was caused by tryptophan residues, and the type belonged to a combined dynamic and static quenching.
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
Dong
D
Wei
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
Bingfeng
B
Wang
School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
Huu Hao
HH
Ngo
School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
Wenshan
W
Guo
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
Fei
F
Han
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
Xiaodong
X
Wang
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China. Electronic address: dubin61@gmail.com.
Bin
B
Du
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Qin
Q
Wei
D065567:Absorption, Physiological / Q000502:physiology; D001420:Bacteria, Aerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001704:Biopolymers / Q000096:biosynthesis*; D004396:Coloring Agents / Q000302:isolation & purification* / Q000378:metabolism*; D003198:Computer Simulation; D008954:Models, Biological; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods
D001704:Biopolymers; D004396:Coloring Agents; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
Aerobic granular sludge; Biosorption; Dye wastewater; Excitation–emission matrix (EEM); Extracellular polymeric substances (EPS)