Generation of aneurogenic larvae by parabiosis of salamander embryos.
Limb regeneration of salamanders is nerve dependent, and the removal of the nerves in early stages of limb regeneration severely curtails the proliferation of the blastemal cells and growth of the regenerate. The removal of the neural tube from a developing salamander embryo results in an aneurogenic larva and the aneurogenic limb (ANL) develops independently without innervation. Paradoxically, the limb in an ANL is capable of regeneration in a nerve-independent manner. Here, we describe a detailed method for the generation of ANL in the spotted salamander, Ambystoma maculatum, for regeneration studies.
Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, UK, anoop.kumar@ucl.ac.uk.
Anoop
A
Kumar
Jean Paul
JP
Delgado
D000822:Animal Husbandry; D000818:Animals; D004625:Embryo, Nonmammalian; D007814:Larva / Q000502:physiology; D010227:Parabiosis / Q000379:methods*; D012038:Regeneration; D014562:Caudata / Q000196:embryology*