Transgenesis in axolotl (Ambystoma mexicanum).
Transgenic animals have been indispensable in elucidating and deciphering mechanisms underlying various biological phenomena. In regeneration, transgenic animals expressing fluorescent protein genes have been crucial for identifying the source cells for regeneration and the mechanism of blastema formation. Animals are usually generated by manipulating their genome using various techniques at/in one cell embryo/fertilized egg stage. Here, we describe the generation of germline transgenic axolotls (Ambystoma mexicanum) using the I-SceI meganuclease and Tol2 transposase.
DFG Center for Regenerative Therapies, Technische Universität Dresden, Fetscherstrasse 105, 01307, Dresden, Germany.
Shahryar
S
Khattak
Elly M
EM
Tanaka
D000558:Ambystoma mexicanum / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000235:genetics; D018014:Gene Transfer Techniques; D007267:Injections; D007814:Larva / Q000235:genetics; D010063:Ovum / Q000378:metabolism; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D019895:Transposases / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D004247:DNA; D019895:Transposases