Effects of a hydroxyapatite-coated nanotube surface of titanium on MC3T3-E1 cells: an in vitro study.
OBJECTIVE
To compare the biological behavior of mouse osteoblast-like cells (MC3T3-E1) on hydroxyapatite (HA)-coated nanotube surface of titanium and plasma-sprayed HA (HA-PS)-coated titanium surface.


METHODS
The HA-coated nanotube surface of titanium were fabricated by anodization coupled with alternative immersion method (AIM). MC3T3-E1 osteoblast cells cultured in vitro were seeded onto these different surfaces; their growth states were examined by a confocal laser scanning microscope; the proliferation behavior, alkaline phosphatase (ALP) activity, osteocalcin (OCN) secretion, and analysis of osteoblastic gene expressions were also compared in detail.


RESULTS
Significant increases in ALP activity and OCN production on days 7 and 14 (P < 0.05) were observed for AIM-coated HA (HA-AIM) surfaces. However, cells cultured on HA-AIM-coated surfaces showed a delayed growth pattern. Real-time polymerase chain reaction analyses showed significantly higher relative mRNA expression levels of osteoblastic genes (runt-related protein 2, osterix, osteopontin, OCN) in cells cultured on the HA-AIM-coated nanotube surfaces as compared with cells cultured on the HA-PS and baer Ti surfaces.


CONCLUSIONS
The current research showed that the HA-AIM-coated nanotubular Ti surfaces enhance osteoblast differentiation, which had the potential to further improve osseointegration.
*Resident, Department of Oral and Maxillofacial Implantology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China. †Resident, Department of Stomatology, Qianfoshan Hospital, Shandong University, Shandong, People's Republic of China. Currently, a PhD candidate at the Department of Bone Metabolism, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, People's Republic of China. ‡Researcher, School of Materials Science and Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China. §Attending, Department of Oral and Maxillofacial Implantology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China. ‖Professor and Director, Department of Oral and Maxillofacial Implantology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China.
Shi-Chong
SC
Qiao
Juan
J
Du
Jing-Mei
JM
Zhao
Jun-Yu
JY
Shi
Ying-Xin
YX
Gu
Hong-Chang
HC
Lai
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use*; D066298:In Vitro Techniques; D051379:Mice; D018613:Microscopy, Confocal; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D014025:Titanium; D059467:Transcriptome
D015675:Osteocalcin; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase