The effect of co-administration of Lawsonia inermis extract and octreotide on experimental hepatocellular carcinoma.
OBJECTIVE
To investigate the effect of Lawsonia inermis total methanolic extract (LIE) and octreotide (OC) on hepatocellular carcinoma (HCC) progression, depending on somatostatin receptor 2 (SSTR-2) and Alfa fetoprotein (AFP) perturbations.


METHODS
Sixty albino mice, divided into five groups (12/each); all except control were injected with single diethyl nitrosamine (DENA) dose of 90 mg/kg body weight, intraperitoneally (IP). DENA group was killed at the last day of week 18. LIE group was given 200 mg/100 ml drinking water from first day of DENA injection until end of week 18. OC group received OC (0.1 mg/kg body weight, twice daily by subcutaneous injection, SC from the first day of week 17 till end of week 18. LIE + OC was given medications till the last day of week 18. Serum AFP, liver tissue SSTR-2 mRNA, its protein expression, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed.


RESULTS
A significant increase in plasma AFP and hepatic mRNA, associated to liver tissue neoplastic changes, SSTR-2 expression and MDA with decreased hepatic GSH were observed in DENA group. These changes were significantly improved by LIE and/or OC.


CONCLUSIONS
LIE and/or OC treatment has effective chemopreventive action due to their ability to alleviate oxidative stress, desensitizing cellular growth receptor to SST.
Biochemistry Department, Colleges of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt, nabilmohie@yahoo.com.
N M
NM
Abdel-Hamid
O M
OM
Mohafez
M H
MH
Nazmy
A
A
Farhan
K
K
Thabet
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D031564:Lawsonia Plant / Q000737:chemistry*; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D015282:Octreotide / Q000008:administration & dosage / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000235:genetics / Q000378:metabolism
D010936:Plant Extracts; D017481:Receptors, Somatostatin; C492049:Sstr2 protein, mouse; D000509:alpha-Fetoproteins; D015282:Octreotide