Cloning and expression pattern of gsdf during the first maleness reproductive phase in the protandrous Acanthopagrus latus.
Gonadal soma-derived factor (Gsdf) is a new member of the transforming growth factor beta superfamily. As a teleost- and gonad-specific growth factor, several studies indicate that Gsdf plays an important role in early germ cell development. In the present study, for the first time, a 1700-bp long gsdf gene was cloned from a protandrous species, Acanthopagrus latus. We further analyzed the cellular localization and the expression patterns of gsdf in respective testicular and ovarian zones during the first maleness reproductive phase. The results showed that gsdf transcripts were highly expressed in the ovotestis during sex differentiation, and the somatic cells of the testicular zone expressed many more gsdf transcripts than those of the ovarian zone. At the onset of puberty, the gsdf expression levels decreased gradually during spermatogenesis. Conversely, the ovarian zone exhibited a stable increase pattern which was similar to the plasma 17β-estradiol (E2) levels. These results suggested that Gsdf may participates in early germ cell development, e.g. proliferation and differentiation of spermatogonia and oogonia in A. latus.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China.
Yuan
Y
Chen
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China.
Wan Shu
WS
Hong
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China.
Qiong
Q
Wang
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China; Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361005, China. Electronic address: chenshixi@xmu.edu.cn.
Shi Xi
SX
Chen
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D006066:Gonads / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012098:Reproduction / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics*; D012741:Sexual Maturation / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism*
D029941:Fish Proteins; D012333:RNA, Messenger
11-Ketotestosterone; 17β-Estradiol; Acanthopagrus latus; Gonadal soma-derived factor; Protandry