Understanding of altered N-glycosylation-related gene expression in recombinant Chinese hamster ovary cells subjected to elevated ammonium concentration by digital mRNA counting.
To understand the effects of ammonium on N-glycosylation, recombinant Chinese hamster ovary (rCHO) cells that produce the Fc-fusion protein were cultivated in serum-free suspension cultures with 10 mM ammonium addition. The addition of ammonium to the cultures reduced the relative proportion of acidic isoforms and sialic acid content of an Fc-fusion protein. Fifty two N-glycosylation-related gene expressions were assessed by the NanoString nCounter system, which provides a digital readout using custom-designed color-coded probes. Among these queried genes, thirteen genes (gale, nans, gpi, man2a1, b4galt5, b4galt7, st3gal2, st3gal5, glb1, hexa, hexb, neu1, and neu3) were up-regulated over 1.5 times in the culture with ammonium addition after 5 days of culture; however, none of the 54 genes were significantly different after 3 days of culture. In particular, the expression level of neu1 (sialidase-1) and neu3 (sialidase-3), which play a role in reduction of sialylation, increased over 2 times. Likewise, the protein expression levels of sialidase-1 and sialidase-3 determined by Western blot analysis were also increased significantly in the culture with ammonium addition. Transient transfection of neu-1 or neu3-targeted siRNAs significantly improved the sialic acid content of the Fc-fusion protein in the culture with ammonium addition, indicating that the decreased sialic acid content was in part due to the increased expression level of sialidase. Taken together, the results obtained in this study provide a better understanding of the detrimental effect of ammonium on N-glycosylation, especially sialylation, in rCHO cells.
Department of Biological Sciences, KAIST, 335 Gwaha k-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
Tae Kwang
TK
Ha
Biotechnology Process Engineering Center, KRIBB, Ochang, 363-883, Republic of Korea. ygkim@kribb.re.kr.
Yeon-Gu
YG
Kim
Department of Biological Sciences, KAIST, 335 Gwaha k-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea. gyunminlee@kaist.ac.kr.
Gyun Min
GM
Lee
D064751:Ammonium Compounds / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006031:Glycosylation; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
D064751:Ammonium Compounds; D003470:Culture Media; D012333:RNA, Messenger; D011994:Recombinant Proteins
CHO cells; Fc-fusion protein; N-glycosylation; ammonium; nanostring nCounter system