Anxiolytic Effects of Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Higher Basidiomycetes) on Ischemia-Induced Anxiety in Rats.
We investigated the anxiolytic effects Agaricus brasiliensis extract (AbSE) on ischemia-induced anxiety using the plus-maze test and the social interaction test. The animals were treated orally with AbSE (4, 8, and 10 mg/kg/d, respectively) for 30 d, followed by middle cerebral artery occlusion-induced cerebral ischemia. Levels of noradrenaline, dopamine, and serotonin in the cerebral cortex of rats, as well as oxidative stress and plasma corticosterone levels were analyzed, respectively. The rota-rod test was carried out to exclude any false positive results in experimental procedures related to anxiety disorders, and the catalepsy test was carried out to investigate whether AbSE induces catalepsy. Our results demonstrate that oral administration of AbSE presented anxiolytic-like effects in the elevated plus-maze test and the social interaction test. Furthermore, AbSE did not induce extrapyramidal symptoms in the catalepsy test. The mechanism underlying the anxiolytic effect of AbSE might be increased brain monoamine levels and plasma corticosterone levels and decreased oxidative stress in cerebral ischemia/reperfusion rats.
Department of Biochemistry and Molecular Biology, Qiqihar Medical University, Qiqihar Heilongjiang, PR China.
Chunjing
C
Zhang
Shandong College of Chinese Medicine, Shandong Yantai, PR China.
Xiulan
X
Gao
Department of Clinical Pathogenic Microorganisms, Qiqihar Medical University, Qiqihar Heilongjiang, PR China.
Yan
Y
Sun
Qiqihar Medical University, Heilongjiang Qiqihar, China.
Xiaojie
X
Sun
Qiqihar Medical University, Heilongjiang Qiqihar, China.
Yanmin
Y
Wu
Heilongjiang Nursing College, Heilongjiang Harbin, China.
Ying
Y
Liu
Department of Biochemistry and Molecular Biology, Harbin Medical University, Heilongjiang Harbin; Department of Biology Genetics, Qiqihar Medical University, Qiqihar Heilongjiang, PR China.
Haitao
H
Yu
Departments of Clinical Psychology, Qiqihar Medical University, Heilongjiang Qiqihar, China.
Guangcheng
G
Cui
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage*; D001007:Anxiety / Q000188:drug therapy* / Q000378:metabolism / Q000523:psychology; D001522:Behavior, Animal; D006801:Humans; D007511:Ischemia / Q000150:complications*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D014151:Anti-Anxiety Agents; D010936:Plant Extracts