NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3.
NgR1, a Nogo receptor, is involved in inhibition of neurite outgrowth and axonal regeneration and regulation of synaptic plasticity. P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. Neuronally differentiated P19 cells expressed the mRNA and protein of NgR1, which could stimulate the phosphorylation of STAT3 when activated by Nogo-P4 peptide, an active segment of Nogo-66. During the whole period of differentiation, mRNAs of all of the NMDA receptor subtypes tested (NR1, NR2A-2D) were consistently expressed, which meant that neuronally differentiated P19 cells maintained some characteristics of neurons, especially central nervous system neurons. Our results suggests that neuronally differentiated P19 cells expressing NgR1 may be an efficient and convenient in vitro model for studying the molecular mechanism of cellular events that involve NgR1 and its binding partners, and for screening compounds that activate or inhibit NgR1.
College of Pharmacy, Korea University, Sejong 339-700, Korea.
Su In
SI
Lee
Bioevaluation Center, KRIBB, Ochang 363-883, Korea.
Jieun
J
Yun
College of Pharmacy, Korea University, Sejong 339-700, Korea.
Ji-Young
JY
Baek
College of Pharmacy, Korea University, Sejong 339-700, Korea.
Yun-Ji
YJ
Jeong
Bioevaluation Center, KRIBB, Ochang 363-883, Korea.
Jin-Ah
JA
Kim
Bioevaluation Center, KRIBB, Ochang 363-883, Korea.
Jong Soon
JS
Kang
College of Pharmacy, Chungbuk National University, Cheongju 362-763, Korea.
Sun Hong
SH
Park
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Sang Kyum
SK
Kim
College of Pharmacy, Korea University, Sejong 339-700, Korea. ; Research Driven Hospital, Korea University Guro Hospital, Biomedical Research Center, Seoul 152-703, Korea.
Song-Kyu
SK
Park
NMDA receptor; Neuronal differentiation; Nogo receptor; P19 cell; STAT3