A comparison of the effects of tributyltin chloride and triphenyltin chloride on cell proliferation, proapoptotic p53, Bax, and antiapoptotic Bcl-2 protein levels in human breast cancer MCF-7 cell line.
In recent years it was disclosed, that numerous organotin(IV) derivatives have remarkable cytotoxicity against several types of cancer cells. The property to inhibit cell growth makes these compounds promising for antitumor therapy, as the clinical effectiveness of cisplatin is limited by drug resistance and significant side effects. Tributyltin and triphenyltin are known as endocrine disruptors. Moreover, the compounds exert their toxicity in mammals predominantly through nuclear receptor signaling. Here we present the effects of tributyltin chloride (TBT-Cl) and triphenyltin chloride (TPT-Cl) on cell proliferation, expression of proapoptotic p53, Bax, and antiapoptotic Bcl-2 proteins in human breast cancer MCF-7 cell line. Dose and time dependent (24, 48 and 72 h) cell expositions have demonstrated TBT-Cl as more effective in inhibiting MCF-7 cell proliferation than TPT-Cl. Short time treatment with TBT-Cl displayed marked stimulation of p53 protein expression when compared to TPT-Cl. Both organotin compounds displayed similar mild enhancement of Bax protein expression. The 24h exposition of TPT-Cl induced substantial diminution of Bcl-2 protein expression in comparison with both, untreated cells and TBT-Cl treated cells. Our observations indicate that TBT-Cl and TPT-Cl have different antiproliferative potency and distinct impact on expression of apoptosis marker proteins.
Institute of Experimental Endocrinology, Laboratory of Cell Endocrinology, Slovak Academy of Sciences, Vlarska 3, 83306 Bratislava, Slovak Republic. Electronic address: maria.fickova@savba.sk.
Maria
M
Fickova
Institute of Experimental Endocrinology, Laboratory of Cell Endocrinology, Slovak Academy of Sciences, Vlarska 3, 83306 Bratislava, Slovak Republic.
Ladislav
L
Macho
Institute of Experimental Endocrinology, Laboratory of Cell Endocrinology, Slovak Academy of Sciences, Vlarska 3, 83306 Bratislava, Slovak Republic.
Julius
J
Brtko
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009947:Organotin Compounds / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D014220:Trialkyltin Compounds / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
D009947:Organotin Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; D014220:Trialkyltin Compounds; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C011559:tributyltin; C012594:triphenyltin chloride
Antiproliferative effects; Apoptosis; Human breast carcinoma cells; Organotin compounds