Basic techniques in mammalian cell tissue culture.
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.
Hayward Genetics Program and Department of Pediatrics, Tulane University School of Medicine, New Orleans, Louisiana.
Katy
K
Phelan
Genetic Diagnostic Laboratory, Children's Hospital at Erlanger, Chattanooga, Tennessee.
Kristin M
KM
May
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005615:Freezing; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D013194:Staining and Labeling; D014186:Transportation; D014357:Trypsin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; D014357:Trypsin
aseptic technique; freezing cells; mammalian cells; medium; passaging cells; tissue culture