Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line.
Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription factor that stimulates cell proliferation. The ethanol saraphi seed (ESS) extract was fractionated using n-hexane, ethyl acetate, n-butanol and water to obtain n-hexane saraphi seed (HSS), ethyl acetate saraphi seed (EASS), n-butanol saraphi seed (BSS), and water saraphi seed (WSS) extracts, respectively. The ESS, HSS and EASS extracts had strong cytotoxic effects on K562 cells in the MTT assay. All three fractions decreased WT1 protein levels and decreased total cell numbers. The HSS extract decreased the WT1 protein levels in a time- and dose-dependent manner. HPLC and NMR analyses indicated that the active compound of HSS was mammea E/BB. M. siamensis seeds are thus identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression.
a Department of Pharmaceutical Science , Faculty of Pharmacy, Chiang Mai University , Chiang Mai 50200 , Thailand.
Methee
M
Rungrojsakul
b Department of Chemistry , Faculty of Science, Chiang Mai University , Chiang Mai 50200 , Thailand.
Aroonchai
A
Saiai
a Department of Pharmaceutical Science , Faculty of Pharmacy, Chiang Mai University , Chiang Mai 50200 , Thailand.
Chadarat
C
Ampasavate
c Department of Medical Technology , Faculty of Associated Medical Sciences, Chiang Mai University , Chiang Mai 50200 , Thailand.
Songyot
S
Anuchapreeda
a Department of Pharmaceutical Science , Faculty of Pharmacy, Chiang Mai University , Chiang Mai 50200 , Thailand.
Siriporn
S
Okonogi
D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D031252:Mammea / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D025721:WT1 Proteins / Q000378:metabolism*
D003374:Coumarins; C554271:Mammea E-BB compound; D010936:Plant Extracts; D025721:WT1 Proteins; C583751:WT1 protein, human
K562; Mammea siamensis; Wilms' tumour 1; leukaemia; mammea E/BB