Mutated myocilin and heterozygous Sod2 deficiency act synergistically in a mouse model of open-angle glaucoma.
Glaucoma is a multifactorial optic neuropathy characterized by retinal ganglion cell (RGC) death and axonal degeneration leading to irreversible blindness. Mutations in the myocilin (MYOC) gene are the most common genetic factors of primary open-angle glaucoma. To develop a genetic mouse model induced by the synergistic interaction of mutated myocilin and another significant risk factor, oxidative stress, we produced double-mutant mice (Tg-MYOC(Y437H/+)/Sod2(+/-)) bearing human MYOC with a Y437H point mutation and a heterozygous deletion of the gene for the primary antioxidant enzyme, superoxide dismutase 2 (SOD2). Sod2 is broadly expressed in most tissues including the trabecular meshwork (TM) and heterozygous Sod2 knockout mice exhibit the reduced SOD2 activity and oxidative stress in all studied tissues. Accumulation of Y437H myocilin in the TM induced endoplasmic reticulum stress and led to a 45% loss of smooth muscle alpha-actin positive cells in the eye drainage structure of 10- to 12-month-old Tg-MYOC(Y437H/+)/Sod2(+/-) mice as compared with wild-type littermates. Tg-MYOC(Y437H/+)/Sod2(+/-) mice had higher intraocular pressure, lost about 37% of RGCs in the peripheral retina, and exhibited axonal degeneration in the retina and optic nerve as compared with their wild-type littermates. Single-mutant littermates containing MYOC(Y437H/+) or Sod2(+/-) exhibited no significant pathological changes until 12 months of age. Additionally, we observed elevated expression of endothelial leukocyte adhesion molecule-1, a human glaucoma marker, in the TM of Tg-MYOC(Y437H/+)/Sod2(+/-) mice. This is the first reported animal glaucoma model that combines expression of a glaucoma-causing mutant gene and an additional mutation mimicking a deleterious environment factor that acts synergistically.
Section on Retinal Ganglion Cell Biology, Laboratory of Retinal Cell and Molecular Biology and.
Myung Kuk
MK
Joe
Section on Retinal Ganglion Cell Biology, Laboratory of Retinal Cell and Molecular Biology and.
Naoki
N
Nakaya
Histology Core, National Eye Institute, NIH, Bethesda, MD 20895, USA.
Mones
M
Abu-Asab
Section on Retinal Ganglion Cell Biology, Laboratory of Retinal Cell and Molecular Biology and tomarevs@nei.nih.gov.
Stanislav I
SI
Tomarev
D000199:Actins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001369:Axons / Q000378:metabolism / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D004843:Epistasis, Genetic; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D005902:Glaucoma, Open-Angle / Q000175:diagnosis / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D057895:Haploinsufficiency; D006579:Heterozygote; D007429:Intraocular Pressure; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009901:Optic Nerve Diseases / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000172:deficiency*; D014129:Trabecular Meshwork / Q000378:metabolism; D000099179:Myocilin; D000099156:Superoxide Dismutase 2
D000199:Actins; D003598:Cytoskeletal Proteins; D019040:E-Selectin; D005136:Eye Proteins; D006023:Glycoproteins; D013482:Superoxide Dismutase; D000099179:Myocilin; D000099156:Superoxide Dismutase 2