Association between cationic liposomes and low molecular weight hyaluronic acid.
This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential.
†Brazilian Synchrotron Light Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
Antonio A M
AA
Gasperini
†Brazilian Synchrotron Light Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.|‡Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo, Rua Talim, 330, CEP 12.231-280, São José dos Campos, São Paulo, Brazil.
Ximena E
XE
Puentes-Martinez
§School of Chemical Engineering, University of Campinas, UNICAMP, PO box 6066, CEP 13.083-970, Campinas, São Paulo, Brazil.
Tiago Albertini
TA
Balbino
∥Centro Universitário das Faculdades Associadas de Ensino UNIFAE, Largo Engenheiro Paulo de Almeida Sandeville, 15, CEP 13.870-377, São João da Boa Vista, São Paulo, Brazil.
Thais de Paula
Tde P
Rigoletto
§School of Chemical Engineering, University of Campinas, UNICAMP, PO box 6066, CEP 13.083-970, Campinas, São Paulo, Brazil.
Gabriela de Sá Cavalcanti
Gde S
Corrêa
⊥Brazilian Nanotechnology National Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
Alexandre
A
Cassago
⊥Brazilian Nanotechnology National Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
Rodrigo Villares
RV
Portugal
§School of Chemical Engineering, University of Campinas, UNICAMP, PO box 6066, CEP 13.083-970, Campinas, São Paulo, Brazil.
Lucimara Gaziola
LG
de La Torre
†Brazilian Synchrotron Light Laboratory, CNPEM, Caixa Postal 6192, CEP 13.083-970, Campinas, São Paulo, Brazil.
Leide P
LP
Cavalcanti
D002412:Cations / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008970:Molecular Weight; D010714:Phosphatidylethanolamines / Q000737:chemistry
D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D006820:Hyaluronic Acid