The effect of 595 nm pulsed dye laser on connective tissue growth factor (CTGF) expression in cultured keloid fibroblasts.
OBJECTIVE
To investigate the effect of pulsed dye laser (PDL 595 nm) on the proliferation and expression of connective tissue growth factor (CTGF) in cultured keloid fibroblasts.


METHODS
Cultured keloid fibroblasts were exposed to pulsed dye laser irradiation at fluences of 6, 8, and 10 J/cm(2) , with pulse durations of 1.5, 3, and 10 ms. The viability of keloid fibroblasts was measured with CCK-8 at 72, 24, and 12 hours prior to irradiation. Subsequently, viability was measured at 12, 24, and 72 hours post-irradiation. Additionally, the fibroblast cell cycle and apoptosis rate were measured by flow cytometry. Finally, keloid fibroblasts underwent real-time polymerase chain reaction (PCR) and Western blot to investigate the CTGF mRNA and protein expression after PDL irradiation. The untreated cultured keloid fibroblasts served as controls.


RESULTS
The proliferation of keloid fibroblasts was significantly inhibited after PDL irradiation. Both CTGF mRNA and protein expression were significantly down-regulated in 1.5, 3, and 10 ms pulse duration groups, in a dose dependent manner (P < 0.05). However, there was no statistically significant difference between groups of different pulse duration in 6, 8, and 10 J/cm(2) fluence ranges (P > 0.05).


CONCLUSIONS
Within certain fluence ranges, pulsed dye laser can effectively suppress the growth of keloids and significantly down-regulate CTGF mRNA and CTGF expression.
Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, 200040, China; Department of Dermatology, Huadong Hospital, Fudan University, Shanghai, 200040, China.
Rongyi
R
Zhu
Baishuang
B
Yue
Qiaorong
Q
Yang
Ying
Y
Ma
Guiqin
G
Huang
Ming
M
Guan
Mathew M
MM
Avram
Zhong
Z
Lu
D017209:Apoptosis / Q000528:radiation effects; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D054025:Lasers, Dye; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor
connective tissue growth factor; culture; keloid; keloid fibroblast; pulsed dye laser; scar; wound healing