Bone regeneration with a collagen model polypeptides/α-tricalcium phosphate sponge in a canine tibia defect model.
BACKGROUND
We evaluated the effects of synthesized collagen model polypeptides consisting of a proline-hydroxyproline-glycine (poly(PHG)) sequence combined with porous alpha-tricalcium phosphate (α-TCP) particles on bone formation in a canine tibia defect model.


METHODS
The porous α-TCP particles were mixed with a poly(PHG) solution, and the obtained sponge was then cross-linked and characterized by x-ray diffraction and scanning electron microscopy. Tibia defects were analyzed in 12 healthy beagles using microcomputed tomography and histological evaluation.


RESULTS
At 2 and 4 weeks, the volume density of new bone was higher in the poly(PHG)/α-TCP group than in poly(PHG) alone group (P < 0.05); however, there was no difference at 8 weeks (P > 0.05). Histological evaluation at 4 weeks after implantation revealed that the poly(PHG) had degraded, and newly formed bone was present on the surface of the α-TCP particles. At 8 weeks, continuous cortical bone formation with a Haversian structure covered the top of the bone defects in both groups.


CONCLUSIONS
This study demonstrates that the composite created using porous α-TCP particles and poly(PHG) is sufficiently adaptable for treating bone defects.
*First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan. †Department of Biomaterials, Osaka Dental University, Osaka, Japan. ‡Department of Oral Implantology, Osaka Dental University, Osaka, Japan.
Tomohiko
T
Ito
Yoshiya
Y
Hashimoto
Shunsuke
S
Baba
Tomio
T
Iseki
Shosuke
S
Morita
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods; D002130:Calcium Phosphates / Q000627:therapeutic use*; D003094:Collagen / Q000627:therapeutic use*; D004285:Dogs; D010455:Peptides / Q000627:therapeutic use*; D010521:Periosteum / Q000601:surgery; D015503:Surgical Sponges; D013977:Tibia / Q000000981:diagnostic imaging / Q000502:physiology* / Q000637:transplantation*; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
D018786:Bone Substitutes; D002130:Calcium Phosphates; D010455:Peptides; C485828:alpha-tricalcium phosphate; C538997:poly(prolyl-hydroxyprolyl-glycyl); D003094:Collagen