Distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing DNA dataset analysis.
This study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. Samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. The 13 samples were sequenced using an Illumina Hi-Seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. Bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. All samples were cultures for fungal growth. The sequencing data revealed that Aspergillus flavus and Aspergillus oryzae were the dominant fungal strains in the rhinosinusitis samples, and Bacillus cereus and Bacillus thuringiensis were the dominant bacterial strains. Indeed, the fungi and bacteria were associated with the development of fungal rhinosinusitis. Furthermore, B. cereus and B. thuringiensis may cooperate with Pseudomonas aeruginosa to inhibit the growth of fungal mycelia. Knowledge of the microbial composition can provide a diagnostic reference for patients with maxillary sinusitis.
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China; Department of Otolaryngology, Daqing Oilfield General Hospital, Daqing, Heilongjiang Province 163001, China.
Junyi
J
Zhang
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China. Electronic address: yunchuanlii@163.com.
Yunchuan
Y
Li
Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Xinxin
X
Lu
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China.
Xiangdong
X
Wang
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China.
Hongrui
H
Zang
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China.
Tong
T
Wang
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China.
Bing
B
Zhou
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren hospital, Capital Medical University, Beijing 100730, China.
Luo
L
Zhang
D000328:Adult; D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D005658:Fungi / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D015523:Maxillary Sinusitis / Q000235:genetics / Q000382:microbiology*; D008875:Middle Aged; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004271:DNA, Fungal
Bacteria; Fungi; High-throughput sequencing; Maxillary sinusitis