[Expression of CD133 and Notch1 in non-small cell lung cancer and the clinicopathological significance].
OBJECTIVE
To investigate the presence of cancer stem cells (CSCs) exist in non-small cell lung cancer (NSCLC) and explore the relationship among the expressions of CD133, Notch1, and vascular endothelial growth factor (VEGF) and their relations with the clinicopathological parameters of the patients.


METHODS
A total of 305 specimens of NSCLC and 80 normal lung tissue specimens were analyzed for CD133, Notch1, and VEGF protein expressions by immunohistochemical staining.


RESULTS
In NSCLC specimens, the positivity rates of CD133, Notch1, and VEGF were 48.9%, 43.9%, and 45.6%, respectively, significantly higher than those in normal lung tissues (10.0%, 15.0%, and 0%, respectively, P<0.01). The expression levels of CD133, Notch1, and VEGF proteins were significantly correlated with the tumor grades, lymph node metastasis, TNM stages, and postoperative survival time of the patients (P<0.01). A positive correlation was found among the expression levels of CD133, Notch1, and VEGF proteins. Kaplan-Meier survival analysis showed a significantly lower overall mean survival time of the patients positive for CD133, Notch1, and VEGF than that of the negative patients (P<0.001). Cox regression analysis suggested that positive expressions of CD133 and Notch1 were independent prognostic factors of NSCLC (P<0.05).


CONCLUSIONS
CD133, Notch1, and VEGF may play important roles in the occurrence, progression, invasion, and metastasis of NSCLC. CD133 and Notch1 have important values for predicting the prognosis and evaluating disease progression of the patients.
Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.E-mail: zhou_lei03@163.com.
Lei
L
Zhou
Shiwu
S
Wu
Lan
L
Yu
Xiaomeng
X
Gong
Wenqing
W
Song
Zenong
Z
Cheng
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D008207:Lymphatic Metastasis; D014411:Neoplastic Stem Cells / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011379:Prognosis; D051881:Receptor, Notch1 / Q000378:metabolism*; D012044:Regression Analysis; D015996:Survival Rate; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C497761:NOTCH1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides; D051881:Receptor, Notch1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A