Desulfuromonas carbonis sp. nov., an Fe(III)-, S0- and Mn(IV)-reducing bacterium isolated from an active coalbed methane gas well.
A novel, mesophilic, obligately anaerobic, acetate-oxidizing, dissimilatory iron-, sulfur-, and manganese-reducing bacterium, designated strain ICBM(T), was obtained from an active, coalbed methane gas well in Indiana, USA. Strain ICBM(T) was a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium that was rich in c-type cytochromes and formed red colonies in solid medium. Strain ICBM(T) conserved energy to support growth from the oxidation of acetate, propionate, pyruvate, malate, fumarate, succinate and dl-lactate, concomitant with dissimilatory iron reduction. Strain ICBM(T) fermented fumarate yielding succinate and acetate. Strain ICBM(T) was able to grow in the temperature range of 10 °C to 37 °C, NaCl concentration range of 0 to 1.2 M, and pH range of 6.5 to 8.0. The physiological characteristics of strain ICBM(T) indicated that it belongs to the Desulfuromonas cluster. The G+C content of its genomic DNA was 61.2 mol%. The predominant cellular fatty acids were C16 : 0 (39.3%), C16 : 1ω7c and/or iso-C15 : 0 2-OH (36.6%). The closest cultured phylogenetic relative of strain ICBM(T) was Desulfuromonas michiganensis BB1(T) with only 95% 16S rRNA gene sequence similarity. This confirmed that strain ICBM(T) is affiliated with the genus Desulfuromonas . On the basis of phenotypic and genotypic differences between strain ICBM(T) and other taxa of the genus Desulfuromonas , strain ICBM(T) represents a novel species for which the name Desulfuromonas carbonis sp. nov. is proposed (type strain ICBM(T) = DSM 29759(T) = JCM 30471(T)). Strain ICBM(T) is the first Fe(III)-, S(0)-, and Mn(IV)-reducing bacterium that was isolated from a coal bed.
School of Public and Environmental Affairs, Indiana University, MSB II, 702 N. Walnut Grove Ave, Bloomington, IN 47405-2204, USA.
Thuy T
TT
An
School of Public and Environmental Affairs, Indiana University, MSB II, 702 N. Walnut Grove Ave, Bloomington, IN 47405-2204, USA.
Flynn W
FW
Picardal
D015373:Bacterial Typing Techniques; D001482:Base Composition; D003580:Cytochromes / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D045287:Desulfuromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005290:Ferric Compounds / Q000378:metabolism; D007196:Indiana; D017895:Manganese Compounds / Q000378:metabolism; D008697:Methane; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003580:Cytochromes; D004269:DNA, Bacterial; D005227:Fatty Acids; D005290:Ferric Compounds; D017895:Manganese Compounds; D012336:RNA, Ribosomal, 16S; D008697:Methane