Impact of high dietary zinc on zinc accumulation, enzyme activity and proteomic profiles in the pancreas of piglets.
The exocrine pancreas plays an important role in zinc homeostasis. Feeding very high (2000-3000mgzinc/kg diet) levels of zinc oxide to piglets for short periods is a common practice in the swine industry to improve performance and prevent diseases. The impact on pancreatic function and possible side effects during long-term feeding of high dietary zinc levels are still poorly understood. A total of 54 weaned piglets were either fed with low (57mg/kg, LZn), normal (164mg/kg, NZn) or high (2425mg/kg, HZn) zinc concentration in the diets. After 4 weeks of feeding, ten piglets per treatment were euthanized and pancreas samples were taken. Tissue zinc concentration and metallothionein abundance was greater with HZn compared with NZn and LZn (P<0.05). Similarly, activity of α-amylase, lipase, trypsin and chymotrypsin was higher with HZn as compared with NZn and LZn diets (P<0.05), whereas elastase activity was unchanged. Total trolox equivalent antioxidative capacity of pancreas tissue was higher with HZn diets compared with the other treatments (P<0.05). Pancreatic protein profiles of NZn and HZn fed piglets were obtained by 2D-DIGE technique and revealed 15 differentially expressed proteins out of 2100 detected spots (P<0.05). The differentially expressed proteins aldose reductase, eukaryotic elongation factor II and peroxiredoxin III were confirmed by immunoblotting. Identified proteins include zinc finger-containing transcription factors and proteins mainly associated with oxidative stress response and signal transduction in HZn compared with NZn pigs. Histologic examination however showed no morphologic changes. The results suggest that long-term supply of very high dietary zinc increases zinc and metallothionein concentration, and digestive enzyme activity, but also triggers oxidative stress reactions in the pancreas of young pigs. The data provide new insights into pancreatic function under outbalanced zinc homeostasis.
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Strasse 49, D-14195 Berlin, Germany. Electronic address: robert.pieper@fu-berlin.de.
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Pieper
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Strasse 49, D-14195 Berlin, Germany.
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Martin
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Strasse 49, D-14195 Berlin, Germany.
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Schunter
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Strasse 49, D-14195 Berlin, Germany.
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Villodre Tudela
Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Takustrasse 3, D-14195 Berlin, Germany.
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Weise
Institute of Veterinary Pathology, Department of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Strasse 15, D-14163 Berlin, Germany.
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Klopfleisch
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Strasse 49, D-14195 Berlin, Germany.
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Zentek
Institute of Veterinary Biochemistry, Department of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, D-14163 Berlin, Germany.
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Einspanier
Institute of Veterinary Biochemistry, Department of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, D-14163 Berlin, Germany.
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Bondzio
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D004032:Diet; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012984:Software; D034421:Sus scrofa; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology*
D000975:Antioxidants; D020543:Proteome; D008668:Metallothionein; D015032:Zinc
Enzyme activity; Oxidative stress; Pancreas; Pigs; Proteomics; Zinc oxide