Use of phosphorodithioate-based compounds as hydrogen sulfide donors.
Synthetic hydrogen sulfide (H2S) donors are useful research tools as well as potential therapeutic agents. In this chapter, we report the detailed protocols for the synthesis and evaluation of a series of phosphorodithioate-based H2S donors. Fluorescence assays were used to determine H2S release from the donors in both aqueous buffers and in cultured cells. These donors were found to be slow-release donors, much like the well-known GYY4137. These donors also showed some protective effects against hydrogen peroxide (H2O2)-induced oxidative damage in myocytes.
Department of Chemistry, Washington State University, Pullman, Washington, USA.
Chung-Min
CM
Park
Department of Chemistry, Washington State University, Pullman, Washington, USA. Electronic address: mxian@wsu.edu.
Ming
M
Xian
D000818:Animals; D002138:Calibration; D002460:Cell Line; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D010710:Phosphates / Q000737:chemistry*; D051381:Rats; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000592:standards
D010710:Phosphates; C083120:phosphorodithioic acid; D006862:Hydrogen Sulfide
Cytoprotection; Donor; Hydrogen sulfide; Hydrolysis; Phosphorodithioate