Dynamic changes in hyperglycosylated human chorionic gonadotrophin throughout the first trimester of pregnancy and its role in early placentation.
OBJECTIVE
What is the in situ localization and function of hyperglycosylated hCG (hCG-H) in first trimester pregnancy tissues?


CONCLUSIONS
HCG-H localizes to the syncytiotrophoblast, cytotrophoblast and invasive extravillous trophoblast within the maternal decidua and promotes invasion during the first trimester of pregnancy.


BACKGROUND
Serum levels of hCG-H decline dramatically throughout the first trimester of pregnancy. As hCG-H is produced by choriocarcinoma cells, it is proposed to regulate trophoblast invasion.


METHODS
Tissues were collected from elective first trimester pregnancy terminations. Placental villous and decidua basalis were collected from Week 6 to Week 12 of gestation (n = 49).


METHODS
Tissues were collected from elective first trimester surgical pregnancy terminations to determine localization, abundance and function of hCG-H. Placental villous outgrowth studies determined the impact of neutralizing endogenous hCG-H on trophoblast function. Real-time proliferation, migration and invasion assays using JEG-3 choriocarcinoma cells further elucidated the role of hCG-H in trophoblast function.


RESULTS
HCG-H localized to syncytiotrophoblast layer of the placental villous from gestational weeks 6-9; thereafter hCG-H localized as a discrete layer between syncytio- and cyto-trophoblast layers. Immunoreactive hCG-H was also observed within the cytotrophoblast layer in Week 7-8 of gestation. HCG-H abundance decreased within placental villous from Weeks 6-12 of gestation (n = 3 placentas per gestational weeks 6-12). HCG-H also localized to anchoring villi within maternal decidua, extravillous trophoblasts invading into the maternal decidua and endovascular trophoblasts remodeling maternal blood vessels. Treatment of primary first trimester villous explants with hCG-H neutralizing antibody reduced trophoblast outgrowth (n = 3 placentas, P < 0.05). Treatment of a trophoblast cell line with neutralizing antibody reduced trophoblast invasion (n = 4, P < 0.05) but did not affect migration or proliferation.


CONCLUSIONS
Functional invasion and migration assays performed using cell lines. Not possible to perform such assays with primary human material.


CONCLUSIONS
HCG-H is an important autocrine factor facilitating trophoblast invasion in the first trimester of pregnancy. Targeting hCG-H may prove useful in the treatment of pathologic pregnancies, such as ectopic pregnancies, or pregnancy complications including pre-eclampsia and gestational trophoblast diseases.


BACKGROUND
This work was supported by the Victorian Government Operational Infrastructure Support Program. J.E. is supported by NHMRC project grant #1047756, L.A.S. and E.D. by NHMRC Fellowships #1002018 and #550905 respectively and E.M. by an NHMRC Early Career Fellowship #611827. The authors have no conflicts of interest relating to this work.
MIMR-PHI Institute, 27-31 Wright St, Clayton, VIC 3168, Australia Department of Physiology, Monash University, Clayton, VIC 3800, Australia jemma.evans@mimr-phi.org.
J
J
Evans
MIMR-PHI Institute, 27-31 Wright St, Clayton, VIC 3168, Australia Department of Obstetrics and Gynecology, Monash University, Clayton, VIC 3800, Australia.
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LA
Salamonsen
MIMR-PHI Institute, 27-31 Wright St, Clayton, VIC 3168, Australia.
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E
Menkhorst
MIMR-PHI Institute, 27-31 Wright St, Clayton, VIC 3168, Australia Department of Anatomy and Development, Monash University, Clayton, VIC 3800, Australia.
E
E
Dimitriadis
D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000097:blood / Q000502:physiology*; D002824:Chorionic Villi / Q000502:physiology; D003656:Decidua / Q000502:physiology; D005260:Female; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000502:physiology*; D010929:Placentation / Q000502:physiology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000502:physiology
D006063:Chorionic Gonadotropin
hyperglycosylated hCG; placentation; trophoblast