Structural phenotyping of stem cell-derived cardiomyocytes.
Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Francesco Silvio
FS
Pasqualini
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Sean Paul
SP
Sheehy
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Ashutosh
A
Agarwal
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Yvonne
Y
Aratyn-Schaus
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA. Electronic address: kkparker@seas.harvard.edu.
Kevin Kit
KK
Parker
D000465:Algorithms; D000818:Animals; D001699:Biometry / Q000379:methods*; D002454:Cell Differentiation; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D000069550:Machine Learning; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D051381:Rats; D013234:Stem Cells / Q000166:cytology*