Factors affecting the accumulation of curcumin in microrhizomes of Curcuma aromatica Salisb.
Curcuminoids, and mainly curcumin, are potential therapeutic agents for the prevention of various diseases; however, little is known about the factors that influence their accumulation in Curcuma species. In this study, the effects of factors such as sucrose concentration, different ratios of 6-benzylaminopurine (6-BA) and α-naphthalene acetic acid (NAA), and light quality on the accumulation of curcumin and other curcuminoids in Curcuma aromatica were investigated. Microrhizomes grown on media containing 3% sucrose produced more curcumin and other curcuminoids than those grown on higher concentrations. Moreover, when compared to other ratios of 6-BA and NAA, microrhizomes induced on 3% sucrose media supplemented with 3.0 mg/L 6-BA and 0.5 mg/L NAA produced more curcumin and other curcuminoids; however, the amount was less than in microrhizomes grown on 3% sucrose alone. We determined that a 5% sucrose medium supplemented with 3.0 mg/L of 6-BA and 0.5 mg/L of NAA enhanced the levels of curcumin and curcuminoids and that exposure to red light further increased production.
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China.
Ke
0000-0002-7639-3221
K
Wu
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China.
Xiaoxia
X
Zhang
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China.
Shulan
S
Sun
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China.
Xiaojing
X
Wang
D001593:Benzyl Compounds; D030024:Curcuma / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003474:Curcumin / Q000378:metabolism*; D007701:Kinetin / Q000494:pharmacology; D008027:Light; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D011687:Purines; D027343:Rhizome / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology
D001593:Benzyl Compounds; D009280:Naphthaleneacetic Acids; D011687:Purines; C034182:1-naphthaleneacetic acid; D013395:Sucrose; D003474:Curcumin; C480551:benzylaminopurine; D007701:Kinetin