MicroRNA 26a modulates regulatory T cells expansion and attenuates renal ischemia-reperfusion injury.
Ischemia-reperfusion injury (IRI) was one of the main causes of acute kidney injury. Mir-26a has been reported to play functions in cellular differentiation, cell growth, cell apoptosis and metastasis. Furthermore, the renal vein levels of Mir-26a were demonstrated to be lower in the poststenotic kidney. However, the effect of Mir-26a on the renal IRI has never been investigated. In our current study, Mir-26a overexpression results in attenuated renal IRI and promoted tregs expansion. The promoted renal function after IRI induced by Mir-26a overexpression was abrogated by depletion of tregs with anti-CD25 antibodies. Mir-26a also significantly suppressed IL-6 expression. And IL-6 overexpression led to significant suppression of the Mir-26a-induced upregulation of Foxp3. Next, we performed additional experiments to determine the therapeutic potential of Mir-26a during the recovery phase after renal IRI. Results showed that Mir-26a treatment after IRI also induced significant expansion of Foxp3(+)CD4(+) Tregs in both spleen and renal on day 10 after IRI. Taken together, our data indicate an important role for Mir-26a in promoting tregs expansion in renal IRI that involving repression of IL-6 expression.
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: smliangchq@126.com.
Simin
S
Liang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Wenxuan
W
Wang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Xin
X
Gou
D058186:Acute Kidney Injury / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D007668:Kidney / Q000276:immunology* / Q000473:pathology; D008212:Lymphocyte Depletion; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015427:Reperfusion Injury / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D015850:Interleukin-6; D035683:MicroRNAs; C543693:Mirn26 microRNA, mouse
IL-6; Mir-26a; Regulatory T cells; Renal IRI