[Caspase-independent programmed cell death induced by temozolomide in rat glioma C6 cell line].
OBJECTIVE
To investigate programmed cell death induced by temozolomide in rat glioma C6 cell line.


METHODS
Rat glioma C6 cell line was treated by temozolomide at different concentrations and for different time lengths. MTT assay was used to evaluate the cell inhibition rate to determine the optimal exposure time and concentration. After the exposure to 400 µg/ml temozolomide for 24 h, the cells were observed for programmed cell death using HE staining, Hochest and Tunnel assay, Western blotting, and immunohistochemistry.


RESULTS
MTT, HE staining, and Hochest and Tunnel assay all showed temozolomide-induced apoptosis in rat glioma C6 cell line. Western blotting and immunohistochemistry revealed up-regulation of Bax and down-regulation of Bcl-2 expression in the exposed cells, where the expressions of caspases 3, 8, 9, and 12 remained unchanged.


CONCLUSIONS
Temozolomide induces apoptosis in rat glioma C6 cell line through a caspase- independent pathway.
Department of Neurology, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.E-mail: chinaylh2003@163.com.
Lihua
L
Liu
Ming
M
Zhang
D000818:Animals; D017209:Apoptosis; D020169:Caspases; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation; D005910:Glioma / Q000473:pathology*; D051381:Rats; D000077204:Temozolomide; D015854:Up-Regulation
D003606:Dacarbazine; D020169:Caspases; D000077204:Temozolomide