Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta).
Four Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from a faecal sample of a 5-year-old ring-tailed lemur (Lemur catta). The strains showed a peculiar morphology, resembling a small coiled snake, a ring shape, or forming a little 'Y' shape. The isolated strains appeared identical, and LMC 13T was chosen as a representative strain and characterized further. Strain LMC 13T showed an A3β peptidoglycan type, similar to that found in Bifidobacterium longum. The DNA base composition was 57.2 mol% G+C. Almost-complete 16S rRNA, hsp60, rpoB, dnaJ, dnaG, purF, clpC and rpoC gene sequences were obtained, and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strain LMC 13T showed the highest similarity to B. longum subsp. suis ATCC 27533T (96.65 %) and Bifidobacterium saguini DSM 23967T (96.64 %). Strain LMC 13T was located in an actinobacterial cluster and was more closely related to the genus Bifidobacteriumthan to other genera in the Bifidobacteriaceae. On the basis of these results, strain LMC 13T represents a novel species within the genus Bifidobacterium, for which the name Bifidobacterium lemurum sp. nov. is proposed; the type strain is LMC 13T ( = DSM 28807T = JCM 30168T).
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Monica
M
Modesto
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Samanta
S
Michelini
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Ilaria
I
Stefanini
Natura Viva Garda Zoological Park S.r.l, Bussolengo, Verona, Italy.
Camillo
C
Sandri
Natura Viva Garda Zoological Park S.r.l, Bussolengo, Verona, Italy.
Caterina
C
Spiezio
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Annamaria
A
Pisi
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Gianfranco
G
Filippini
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Bruno
B
Biavati
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
Paola
P
Mattarelli
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D007900:Lemur / Q000382:microbiology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S