Oral vaccination of mice with Trichinella spiralis nudix hydrolase DNA vaccine delivered by attenuated Salmonella elicited protective immunity.
We have previously reported that Trichinella spiralis Nudix hydrolase (TsNd) bound to intestinal epithelial cells (IECs), and the vaccination of mice with recombinant TsNd protein (rTsNd) produced a partial protective immunity against challenge infection in mice. In this study, the full-length cDNA sequence of TsNd gene was cloned into the eukaryotic expression plasmid pcDNA3.1, and the recombinant TsNd DNA was transformed into attenuated Salmonella typhimurium strain ⊿cyaSL1344. Oral immunization of mice with TsNd/S. typhimurium elicited a significant local mucosal IgA response and a systemic Th1/Th2 immune response. Cytokine profiling also showed a significant increase in the Th1 (IFN-γ, IL-2) and Th2 (IL-4, 10) responses in splenocytes of immunized mice upon stimulation with the rTsNd. The oral immunization of mice with TsNd/S. typhimurium displayed a statistically significant 73.32% reduction in adult worm burden and a 49.5% reduction in muscle larvae after challenge with T. spiralis muscle larvae, compared with PBS control group. Our results demonstrated that TsNd DNA delivered by attenuated live S. typhimurium elicited a local IgA response and a mixed Th1/Th2 immune response, and produced a partial protection against T. spiralis infection in mice.
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Pei
P
Liu
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China. Electronic address: wangzq@zzu.edu.cn.
Zhong Quan
ZQ
Wang
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Ruo Dan
RD
Liu
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Peng
P
Jiang
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Shao Rong
SR
Long
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Li Na
LN
Liu
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Xin Zhuo
XZ
Zhang
The Key Lab of Animal Disease and Public Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Xiang Chao
XC
Cheng
The Key Lab of Animal Disease and Public Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Chuan
C
Yu
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China.
Hui Jun
HJ
Ren
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China. Electronic address: cuij@zzu.edu.cn.
Jing
J
Cui
D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D016503:Drug Delivery Systems; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011755:Pyrophosphatases / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D017160:Trichinella spiralis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000096964:Nudix Hydrolases
D000909:Antibodies, Helminth; D016207:Cytokines; D011755:Pyrophosphatases; D014612:Vaccines; D019444:Vaccines, DNA; D000096964:Nudix Hydrolases
DNA vaccine; Nudix hydrolase (Nd); Salmonella typhimurium; Secretory IgA; Th1/Th2; Trichinella spiralis