Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint.
Synovial chondromatosis (SC) of temporomandibular joint is rare proliferative disorder featured by the formation of cartilaginous nodules in synovium and joint space. Transforming growth factor beta 3 (TGF-β3) is closely related to chondrogenic differentiation, and might participate in pathogenesis of SC. We discovered that increased quantity of synoviocytes and blood vessels were observed in SC synovium. The vessel wall and sublining fibroblasts were stained positively by the antibodies against TGF-β3, fibroblast growth factor 2 (FGF-2), and CD34. In loose bodies (LBs), TGF-β3 was mainly expressed in chondrocytes and FGF-2 was expressed in chondrocytes, fibroblasts, and vessel walls. Expressions of TGF-β1, TGF-β3, FGF-2, Sox9, Wnt-4, Foxc2, and VEGF-A mRNA were significantly higher in SC synovium. Stimulation of TGF-β3 on synoviocytes increased alkaline phosphatase (ALP) activity and expressions of chondrogenic genes (Sox9, Col2α1, Aggrecan, Wnt-4, and Wnt-11), osteogenic genes (Runx2, Foxc2, osteocalcin, and Col1α1), and VEGF-A, but failed to influence FGF-2 expression. However, the addition of FGF-2 increased TGF-β3 expression. In conclusion, TGF-β3 existed in synovium and LBs of SC, and was responsible for the pathogenesis of SC.
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Yingjie
Y
Li
Department of Orthodontics, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Loaye Abdelaziz
LA
El Mozen
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Hengxing
H
Cai
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Wei
W
Fang
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Qinggong
Q
Meng
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Jian
J
Li
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Mohong
M
Deng
Department of Oral and Maxillofacial Surgery, The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School &Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Xing
X
Long
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015838:Chondromatosis, Synovial / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology; D013704:Temporomandibular Joint / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D053782:Transforming Growth Factor beta3 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D053782:Transforming Growth Factor beta3; D016222:Fibroblast Growth Factor 2