Gene expression by simian virus 40 large T antigen-induced medulloblastomas in mice.
Signaling pathways known to have components with mutations in human medulloblastoma include sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor. Microarray analysis was applied to examine the gene expression changes in medulloblastomas of pTet-on/pTRE-SV40Tag transgenic mice. Altogether, 14 112 genes were detectable, including 152 genes with significantly different expression levels. These genes were associated with immunity, the cell cycle, signal transduction, cytoskeleton and metabolism. To further confirm the microarray data, real-time polymerase chain reactions were used to examine the expression changes of genes related to sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor signal pathways. Immunohistochemistry detected insulin receptor substrate-1 in the nuclei of brain tumor tissue cells from pTet-on/pTRE-SV40Tag transgenic mice, suggesting that SV40 large T antigen may activate the insulin-like growth factor signal pathway to promote tumorigenesis.
Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, Key Laboratory of Brain Functional Genomics, MOE & STCSM, East China Normal University, Shanghai 200062, China.
Xiaoluan
X
Wei
Shanghai Laboratory Animal Research Center, Shanghai 200032, China.
Jie
J
Feng
Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, Key Laboratory of Brain Functional Genomics, MOE & STCSM, East China Normal University, Shanghai 200062, China.
Yinghe
Y
Hu
SV40 large T antigen; cDNA microarray; insulin-like growth factor; medulloblastoma; real-time polymerase chain reaction