Highly sensitive cell imaging "Off-On" fluorescent probe for mitochondria and ATP.
A smart Off-On molecular scaffold/fluorescent probe 1 has been designed and synthesized. The probe has shown considerable photostability, cell permeability, organelle specificity and selectivity for ATP. The multicolor live cell imaging experiments in HeLa cells showed high selectivity of probe 1 for mitochondria with fluorescence "turn-on" response. As a proof of concept and promising prospects for application in biological sciences probe 1 has been utilized to detect ATP sensitively in a partial aqueous medium and intracellularly in HeLa cells. The favorable interaction between triphosphate unit of ATP and piperazine N atoms of probe 1 is attributed to synergistic effects of H-bonding and electrostatic interactions that encouraged the CH-π and π→π stacking between anthracene and purine rings. Consequently, the observed enhanced "turn-on" emission and a naked-eye sensitive blue-green color in the medium is attributable to arrest in photoinduced electron transfer (PET) process.
Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Priyanka
P
Srivastava
Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Syed S
SS
Razi
Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Rashid
R
Ali
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Saurabh
S
Srivastav
Indian Institute of Toxicology and Research (IITR), Lucknow 226001, UP, India.
Satyakam
S
Patnaik
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Saripella
S
Srikrishna
Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India. Electronic address: arvindmisra2003@yahoo.com.
Arvind
A
Misra
D000255:Adenosine Triphosphate / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006367:HeLa Cells; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D000069416:Intravital Microscopy / Q000379:methods; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D057054:Molecular Imaging / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate
ATP; Biosensor; Fluorescence; Live cell imaging; Mitochondria; PET