Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane.
The aim of this work was to study the impact of environmental factors on the bioremediation of Cr(VI) and lindane contaminated soil, by an actinobacterium, Streptomyces sp. M7, in order to optimize the process. Soil samples were contaminated with 25 µg kg(-1) of lindane and 50 mg kg(-1) of Cr(VI) and inoculated with Streptomyces sp. M7. The lowest inoculum concentration which simultaneously produced highest removal of Cr(VI) and lindane was 1 g kg(-1). The influence of physical and chemical parameters was assessed using a full factorial design. The factors and levels tested were: Temperature: 25, 30, 35°C; Humidity: 10%, 20%, 30%; Initial Cr(VI) concentration: 20, 50, 80 mg kg(-1); Initial lindane concentration: 10, 25, 40 µg kg(-1). Streptomyces sp. M7 exhibited strong versatility, showing the ability to bioremediate co-contaminated soil samples at several physicochemical conditions. Streptomyces sp. M7 inoculum size was optimized. Also, it was fitted a model to study this process, and it was possible to predict the system performance, knowing the initial conditions. Moreover, optimum temperature and humidity conditions for the bioremediation of soil with different concentrations of Cr(VI) and lindane were determined. Lettuce seedlings were a suitable biomarker to evaluate the contaminants mixture toxicity. Streptomyces sp. M7 carried out a successful bioremediation, which was demonstrated through ecotoxicity test with Lactuca sativa.
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET. Av. Belgrano y Pasaje Caseros. 4000 Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 491, 4000 Tucumán, Argentina.
JuanDaniel
J
Aparicio
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET. Av. Belgrano y Pasaje Caseros. 4000 Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 491, 4000 Tucumán, Argentina.
María Zoleica Simón
MZ
Solá
Unidad de Administración Territorial, Centro Científico Tecnológico, CCT-CONICET-Tucumán, Crisóstomo Álvarez 722, 4000 Tucumán, Argentina; Universidad del Norte Santo Tomás de Aquino (UNSTA), 4000 Tucumán, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Catamarca. Esquiú 799, 4700 Catamarca, Argentina.
Claudia Susana
CS
Benimeli
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET. Av. Belgrano y Pasaje Caseros. 4000 Tucumán, Argentina; Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 491, 4000 Tucumán, Argentina; Universidad del Norte Santo Tomás de Aquino (UNSTA), 4000 Tucumán, Argentina.
María Julia
MJ
Amoroso
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET. Av. Belgrano y Pasaje Caseros. 4000 Tucumán, Argentina; Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán. Miguel Lillo 205, 4000 Tucumán, Argentina. Electronic address: mpolti@proimi.org.ar.
Marta Alejandra
MA
Polti
D001673:Biodegradation, Environmental; D002857:Chromium / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D001556:Hexachlorocyclohexane / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D013302:Streptomyces / Q000378:metabolism*; D013696:Temperature
D012987:Soil; D012989:Soil Pollutants; D002857:Chromium; C074702:chromium hexavalent ion; D001556:Hexachlorocyclohexane
Actinobacteria; Bioremediation; Chromium; Lindane; Soil