Angiogenic tube formation of bovine aortic endothelial cells grown on patterns formed by H2/He plasma treatment of the plasma polymerized hexamethyldisiloxane film.
Angiogenesis, the process to generate new vessels, is necessary for normal development in children as well as the wound healing and the tumor growth in adults. Therefore, it is physiologically and/or pathophysiologically significant to monitor angiogenesis. However, classical in vitro methods to evaluate angiogenesis take a long time and are expensive. Here, the authors developed a novel method to analyze the angiogenesis in a simple and economical way, using patterned films. In this study, the authors fabricated a plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film deposited by capacitively coupled plasma chemical vapor deposition system with various plasma powers. The patterned PPHMDSO film was plasma treated by 10:90 H2/He mixture gas through a metal shadow mask. The films were characterized by water contact angle, atomic force microscopy, x-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy analyses. Our results show that the PPHMDSO film suppresses the cell adhesion, whereas surface modified PPHMDSO film enhances the cell adhesion and proliferation. From cell culture experiments, the authors found that the patterned film with 300 μm line interval was most efficient to evaluate the tube formation, a sapient angiogenic indicator. This patterned film will provide an effective and promising method for evaluating angiogenesis.
Brain Korea 21 Physics Research Division, Department of Physics, Institute of Basic Science, Sungkyunkwan University, Suwon 440-746, South Korea.
Jisoo
J
Park
Brain Korea 21 Physics Research Division, Department of Physics, Institute of Basic Science, Sungkyunkwan University, Suwon 440-746, South Korea.
Myunghoon
M
Ha
Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University, Yongin 448-701, South Korea.
Hye-Rim
HR
Lee
Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University, Yongin 448-701, South Korea.
Heonyong
H
Park
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, South Korea.
Jung-Hoon
JH
Yu
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, South Korea.
Jin-Hyo
JH
Boo
Brain Korea 21 Physics Research Division, Department of Physics, Institute of Basic Science, Sungkyunkwan University, Suwon 440-746, South Korea.
Donggeun
D
Jung
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic; D058626:Plasma Gases; D012833:Siloxanes
D058626:Plasma Gases; D012833:Siloxanes; C015917:hexamethyldisiloxane