Serum biomarkers for early detection of hepatocellular carcinoma associated with HCV infection in egyptian patients.
BACKGROUND
Early detection of hepatocellular carcinoma using serological markers with better sensitivity and specificity than alpha fetoprotein (AFP) is needed.


OBJECTIVE
The aim of this study was to evaluate the diagnostic value of serum sICAM-1, β-catenin, IL-8, proteasome and sTNFR-II in early detection of HCC.


METHODS
Serum levels of IL-8, sICAM-1, sTNFR-II, proteasome and β-catenin were measured by ELISA assay in 479 serum samples from 192 patients with HCC, 96 patients with liver cirrhosis (LC), 96 patients with chronic hepatitis C (CHC) and 95 healthy controls.


RESULTS
Serum levels of proteasome, sICAM-1, β-catenin and αFP were significantly elevated in HCC group compared to other groups (P-value<0.001), where serum level of IL-8 was significantly elevated in the LC and HCC groups compared to CHC and control groups (P-value <0.001), while no significant difference was noticed in patients with HCC and LC (P-value=0.09). Serum level of sTNFR- II was significantly elevated in patients with LC compared to HCC, CHC and control groups (P-value <0.001); also it was significantly higher in HCC compared to CHC and control groups (P-value <0.001). ROC curve analysis of the studied markers between HCC and other groups revealed that the serum level of proteasome had sensitivity of 75.9% and specificity of 73.4% at a cut-off value of 0.32 μg/ml with AUC 0.803 sICAM-1 at cut off value of 778ng/ml, the sensitivity was 75.8% and the specificity was 71.8% with AUC 0.776. β-catenin had sensitivity and specificity of 70% and 68.6% respectively at a cut off value of 8.75ng/ml with an AUC of 0.729. sTNFR-II showed sensitivity of 86.3% and specificity of 51.8% at a cut off value of 6239.5pg/ml with an AUC of 0.722. IL-8 had sensitivity of 70.4% and specificity of 52.3% at a cut off value of 51.5pg/ml with AUC 0.631.


CONCLUSIONS
Our data supported the role of proteasome, sICAM-1, sTNFR-II and β-catenin in early detection of HCC. Also, using this panel of serological markers in combination with αFP may offer improved diagnostic performance over αFP alone in the early detection of HCC.
Virology and Immunolgy Unit, Cancer Biology Department, National Cancer Institute, Cairo, Egypt E-mail : ncizekri@yahoo.com.
Abdel-Rahman
AR
Zekri
Amira Salah El-Din
AS
Youssef
Yasser Mabrouk
YM
Bakr
Reham Mohamed
RM
Gabr
Mahmoud Nour El-Din
MN
El-Rouby
Ibtisam
I
Hammad
Entsar Abd El-Monaem
EA
Ahmed
Hanan Abd El-Haleem
HA
Marzouk
Mohammed Mahmoud
MM
Nabil
Hanan Abd El-Hafez
HA
Hamed
Yasser Hamada Ahmed
YH
Aly
Khaled S
KS
Zachariah
Gamal
G
Esmat
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D016022:Case-Control Studies; D042241:Early Diagnosis; D004534:Egypt; D005260:Female; D005500:Follow-Up Studies; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000097:blood* / Q000150:complications / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve; D055815:Young Adult
D014408:Biomarkers, Tumor