Oxidation of H2S in mammalian cells and mitochondria.
Hydrogen sulfide (H2S) is the third gasotransmitter described in mammals. These gasotransmitters (H2S, CO, and NO) are small molecules able to diffuse freely across membranes and thus susceptible to reach easily intracellular targets, one of which is the respiratory enzyme cytochrome oxidase subject to complete inhibition by low micromolar concentrations of these gases. However in contrast to NO or CO, H2S can be metabolized by a sulfide quinone reductase feeding the mitochondrial respiratory chain with the hydrogen atoms of sulfide. Sulfide is thus a two-sided molecule: substrate or poison according to the concentration. The aim of this chapter is to present a mean to monitor sulfide oxidation by isolated mitochondria or cells and to summarize how the properties of this amazing couple (mitochondria and sulfide) translate into practical and conceptual consequences.
Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.
Abbas
A
Abou-Hamdan
Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.
Hala
H
Guedouari-Bounihi
Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.
Véronique
V
Lenoir
INRA-CRNH-IdF-AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behavior, Paris, France.
Mireille
M
Andriamihaja
INRA-CRNH-IdF-AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behavior, Paris, France.
François
F
Blachier
Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France. Electronic address: frederic.bouillaud@inserm.fr.
Frédéric
F
Bouillaud
D000818:Animals; D002460:Cell Line; D019069:Cell Respiration; D042967:Electron Transport Complex I / Q000378:metabolism; D004734:Energy Metabolism; D057075:Enzyme Assays; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D014451:Ubiquinone / Q000378:metabolism
D014451:Ubiquinone; D042967:Electron Transport Complex I; D006862:Hydrogen Sulfide
Bioenergetics; Hydrogen sulfide; Metabolism; Mitochondria; Oxygen; Respiration; Sulfur