Genomic and phylogenetic characterization of novel, recombinant H5N2 avian influenza virus strains isolated from vaccinated chickens with clinical symptoms in China.
Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Shijiazhuang/1209/2013, A/chicken/Chiping/0321/2014, and A/chicken/Laiwu/0313/2014, were isolated from chickens with clinical symptoms of avian influenza. Complete genomic and phylogenetic analyses demonstrated that all three isolates are novel recombinant viruses with hemagglutinin (HA) and matrix (M) genes derived from H5N1, and remaining genes derived from H9N2-like viruses. The HA cleavage motif in all three strains (PQIEGRRRKR/GL) is characteristic of a highly pathogenic avian influenza virus strain. These results indicate the occurrence of H5N2 recombination and highlight the importance of continued surveillance of the H5N2 subtype virus and reformulation of vaccine strains.
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. hyingxu@163.com.
Huaiying
H
Xu
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China. mfshd@163.com.
Fang
F
Meng
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. hhddhh@163.com.|Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan, Shandong 250100, China. hhddhh@163.com.
Dihai
D
Huang
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. sxd1985wl@163.com.|Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan, Shandong 250100, China. sxd1985wl@163.com.
Xiaodan
X
Sheng
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. wangyouling71@163.com.
Youling
Y
Wang
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. weizhang1128@126.com.
Wei
W
Zhang
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China. wschang@sdau.edu.cn.
Weishan
W
Chang
Animal Diseases Diagnostic Laboratory, Ohio Department of Agriculture, Reynoldsburg, OH 43068, USA. wang.1074@buckeyemail.osu.edu.
Leyi
L
Wang
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. qinzm1997@163.com.|Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan, Shandong 250100, China. qinzm1997@163.com.
Zhuoming
Z
Qin
D000818:Animals; D002645:Chickens; D002681:China; D016679:Genome, Viral; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics; D053125:Influenza A Virus, H5N2 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics; D007252:Influenza Vaccines / Q000008:administration & dosage*; D005585:Influenza in Birds / Q000517:prevention & control / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D007252:Influenza Vaccines; D012367:RNA, Viral