Rhodamine-123: a p-glycoprotein marker complex with sodium lauryl sulfate.
Aim of this study was to investigate the role of sodium lauryl sulfate (SLS) as P-glycoprotein inhibitor. The everted rat gut sac model was used to study in-vitro mucosal to serosal transport of Rhodamine-123 (Rho-123). Surprisingly, SLS decreases the serosal absorption of Rho-123 at all investigated concentrations. Investigation reveals complex formation between Rhodamine-123 and sodium lauryl sulfate. Interaction profile of SLS & Rho-123 was studied at variable SLS concentrations. The SLS concentration higher than critical micelle concentration (CMC) increases the solubility of Rho-123 but could not help in serosal absorption, on the contrary the absorption of Rho-123 decreased. Rho-123 and SLS form pink color complex at sub-CMC. The SLS concentrations below CMC decrease the solubility of Rho-123. For further studies, Rho-123 & SLS complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (DSC). Thermal analysis also proved the formation of complex between SLS & Rho-123. The P values were found to be significant (<0.05) except group comprising 0.0001% SLS, and that is because 0.0001% SLS is seems to be very low to affect the solubility or complexation of Rho-123.
Department Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Abdullah M
AM
Al-Mohizea
Department Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Fahad Ibrahim
FI
Al-Jenoobi
Department Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Mohd Aftab
MA
Alam
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors*; D000818:Animals; D001692:Biological Transport; D015415:Biomarkers; D008297:Male; D008823:Micelles; D051381:Rats; D017207:Rats, Sprague-Dawley; D020112:Rhodamine 123 / Q000737:chemistry* / Q000493:pharmacokinetics; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012995:Solubility
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015415:Biomarkers; D008823:Micelles; D020112:Rhodamine 123; D012967:Sodium Dodecyl Sulfate