Chronic treatment with ginsenoside Rg1 promotes memory and hippocampal long-term potentiation in middle-aged mice.
Ginseng serves as a potential candidate for the treatment of aging-related memory decline or memory loss. However, the related mechanism is not fully understood. In this study, we applied an intraperitoneal injection of ginsenoside Rg1, an active compound from ginseng in middle-aged mice and detected memory improvement and the underlying mechanisms. Our results showed that a period of 30-day administration of ginsenoside Rg1 enhanced long-term memory in the middle-aged animals. Consistent with the memory improvement, ginsenoside Rg1 administration facilitated weak theta-burst stimulation (TBS)-induced long-term potentiation (LTP) in acute hippocampal slices from middle-aged animals. Ginsenoside Rg1 administration increased the dendritic apical spine numbers and area in the CA1 region. In addition, ginsenoside Rg1 administration up-regulated the expression of hippocampal p-AKT, brain-derived neurotrophic factor (BDNF), proBDNF and glutamate receptor 1 (GluR1), but not p-ERK. Interestingly, the phosphatase and tensin homolog deleted on chromosome ten (PTEN) inhibitor (bpV) mimicked the ginsenoside Rg1 effects, including increasing p-AKT expression, promoting hippocampal basal synaptic transmission, LTP and memory. Taken together, our data suggest that ginsenoside Rg1 treatment improves memory in middle-aged mice possibly through regulating the PI3K/AKT pathway, altering apical spines and facilitating hippocampal LTP.
Key Laboratory of Xin'An Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, China. Electronic address: guoqizhu@gmail.com.
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Zhu
Shanghai University of Traditional Chinese Medicine, Shanghai, China.
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Wang
Key Laboratory of Xin'An Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, China.
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Li
Key Laboratory of Xin'An Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, China. Electronic address: wangjian6301@163.com.
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Wang
D000375:Aging / Q000187:drug effects / Q000502:physiology; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005239:Fear / Q000187:drug effects / Q000502:physiology; D036145:Ginsenosides / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D057567:Memory, Long-Term / Q000187:drug effects* / Q000502:physiology; D008810:Mice, Inbred C57BL; D018697:Nootropic Agents / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D018091:Receptors, AMPA / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D046509:Tissue Culture Techniques
D019208:Brain-Derived Neurotrophic Factor; D048049:Extracellular Signal-Regulated MAP Kinases; D036145:Ginsenosides; D018697:Nootropic Agents; D051059:PTEN Phosphohydrolase; D051057:Proto-Oncogene Proteins c-akt; D018091:Receptors, AMPA; C487757:Pten protein, mouse; C035054:ginsenoside Rg1
BDNF; ginsenoside Rg1; long-term potentiation; synapses