Methodology for optimally sized centrifugal partition chromatography columns.
Centrifugal Partition Chromatography (CPC) is a separation process based on the partitioning of solutes between two partially miscible liquid phases. There is no solid support for the stationary phase. The centrifugal acceleration is responsible for both stationary phase retention and mobile phase dispersion. CPC is thus a process based on liquid-liquid mass transfer. The separation efficiency is mainly influenced by the hydrodynamics of the phases in each cell of the column. Thanks to a visualization system, called "Visual CPC", it was observed that the mobile phase can flow through the stationary phase as a sheet, or a spray. Hydrodynamics, which directly governs the instrument efficiency, is directly affected during scale changes, and non-linear phenomena prevent the successful achievement of mastered geometrical scale changes. In this work, a methodology for CPC column sizing is proposed, based on the characterization of the efficiency of advanced cell shapes, taking into account the hydrodynamics. Knowledge about relationship between stationary phase volume, cell efficiency and separation resolution in CPC allowed calculating the optimum cell number for laboratory and industrial scale CPC application. The methodology is highlighted with results on five different geometries from 25 to 5000 mL, for two applications: the separation of alkylbenzene by partitioning with heptane/methanol/water biphasic system; and the separation of peptides by partitioning with n-butanol/acetic acid/water (4/1/5) biphasic system. With this approach, it is possible to predict the optimal CPC column length leading to highest productivity.
GEPEA, UMR CNRS 6144, Université de Nantes, 37 bd de l'Université 44602 Saint Nazaire Cedex, France; Kromaton, Rousselet Centrifugation SA, Annonay, France.
Sébastien
S
Chollet
GEPEA, UMR CNRS 6144, Université de Nantes, 37 bd de l'Université 44602 Saint Nazaire Cedex, France. Electronic address: luc.marchal@univ-nantes.fr.
Luc
L
Marchal
Kromaton, Rousselet Centrifugation SA, Annonay, France.
Jérémy Meucci
ICMR, UMR CNRS 7312, Université de Reims, France.
Jean-Hugues
JH
Renault
GEPEA, UMR CNRS 6144, Université de Nantes, 37 bd de l'Université 44602 Saint Nazaire Cedex, France.
Jack
J
Legrand
GEPEA, UMR CNRS 6144, Université de Nantes, 37 bd de l'Université 44602 Saint Nazaire Cedex, France.
Alain
A
Foucault
D001555:Benzene Derivatives / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D057446:Hydrodynamics; D010446:Peptide Fragments / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D001555:Benzene Derivatives; D010446:Peptide Fragments; D012997:Solvents; D014867:Water
Centrifugal Partition Chromatography; Engineering; Flow pattern; Mass transfer; Scale-up method