The association of serotype and pulsed-field gel electrophoresis genotype in isolates of Streptococcus pneumoniae isolated in Israel.
The relationship between Streptococcus pneumoniae isolates causing invasive infections in children admitted to a single center in central Israel was examined by pulsed-field gel electrophoresis (PFGE) and serotyping. Although there was a close correlation between serotype and PFGE clone, the genetic diversity varied by serotype, with some genotypes comprising multiple serotypes. Additionally, clones C and D were associated with higher penicillin minimum inhibitory concentrations. Serotyping alone may be insufficient for epidemiological mapping of pneumococcal isolates in the era of pneumococcal conjugate polysaccharide vaccines.
Infectious Diseases, Shaare Zedek Medical Center, PO Box 3235, Jerusalem 91031, Israel; Faculty of Medicine, Hebrew University, Jerusalem, Israel. Electronic address: mbarmeir@gmail.com.
M
M
Bar-Meir
Pediatrics, Shaare Zedek Medical Center, Jerusalem, Israel.
G
G
Naaman
Microbiology Laboratory, Shaare Zedek Medical Center, Jerusalem, Israel.
M
M
Assous
Central Laboratories, Ministry of Health, Jerusalem, Israel.
Z
Z
Korenman
Central Laboratories, Ministry of Health, Jerusalem, Israel.
L
L
Valinsky
Faculty of Medicine, Hebrew University, Jerusalem, Israel; Pediatric Pulmonology, Shaare Zedek Medical Center, Jerusalem, Israel.
E
E
Picard
D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D000069443:Heptavalent Pneumococcal Conjugate Vaccine / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007557:Israel; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011018:Pneumonia, Pneumococcal / Q000276:immunology* / Q000517:prevention & control; D065288:Serogroup; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
C538862:13-valent pneumococcal vaccine; D000900:Anti-Bacterial Agents; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D010406:Penicillins; D022242:Pneumococcal Vaccines
Electrophoresis; Gel; Genotype; Pulsed-field; S.pneumoniae; Serotype