Proteomic analysis reveals the mechanisms of Mycena dendrobii promoting transplantation survival and growth of tissue culture seedlings of Dendrobium officinale.
OBJECTIVE
Dendrobium officinale is an important traditional Chinese medicinal herb. Its seedlings generally show low survival and growth when transferred from in vitro tissue culture to a greenhouse or field environment. In this study, the effect of Mycena dendrobii on the survival and growth of D. officinale tissue culture seedlings and the mechanisms involved was explored.


RESULTS
Mycena dendrobii were applied underneath the roots of D. officinale tissue culture seedlings. The seedling survival and growth were analysed. The root proteins induced by M. dendrobii were identified using two-dimensional (2-D) electrophoresis and matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS). Mycena dendrobii treatment significantly enhanced survival and growth of D. officinale seedlings. Forty-one proteins induced by M. dendrobii were identified. Among them, 10 were involved in defence and stress response, two were involved in the formation of root or mycorrhizae, and three were related to the biosynthesis of bioactive constituents.


CONCLUSIONS
These results suggest that enhancing stress tolerance and promoting new root formation induced by M. dendrobii may improve the survival and growth of D. officinale tissue culture seedlings.


CONCLUSIONS
This study provides a foundation for future use of M. dendrobii in the large-scale cultivation of Dendrobiums.
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
X B
XB
Xu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
X Y
XY
Ma
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
H H
HH
Lei
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
H M
HM
Song
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
Q C
QC
Ying
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
M J
MJ
Xu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
S B
SB
Liu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
H Z
HZ
Wang
D000363:Agaricales / Q000254:growth & development / Q000502:physiology*; D031667:Dendrobium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D040901:Proteomics; D036226:Seedlings / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology
D010940:Plant Proteins
Dendrobium; Mycena dendrobii; mycorrhiza; proteome; transplantation