Fast clonal expansion and limited neural stem cell self-renewal in the adult subependymal zone.
We analyzed the progeny of individual neural stem cells (NSCs) of the mouse adult subependymal zone (SEZ) in vivo and found a markedly fast lineage amplification, as well as limited NSC self-renewal and exhaustion in a few weeks. We further unraveled the mechanisms of neuronal subtype generation, finding that a higher proportion of NSCs were dedicated to generate deep granule cells in the olfactory bulb and that larger clones were produced by these NSCs.
1] Institute of Stem Cell Research, Helmholtz Center, Munich, Germany. [2] Biomedical Center, University of Munich, Munich, Germany.
Filippo
F
Calzolari
Institute of Stem Cell Research, Helmholtz Center, Munich, Germany.
Julia
J
Michel
Institute of Stem Cell Research, Helmholtz Center, Munich, Germany.
Emily Violette
EV
Baumgart
1] Institute of Computational Biology, Helmholtz Center, Munich, Germany. [2] Institute for Mathematical Sciences, Technical University Munich, Garching, Germany.
Fabian
0000000224191943
F
Theis
1] Institute of Stem Cell Research, Helmholtz Center, Munich, Germany. [2] Biomedical Center, University of Munich, Munich, Germany. [3] Munich cluster for Systems Neurology (SYNERGY), Ludwig-Maximilians-Universität, München, Germany.
Magdalena
M
Götz
1] Institute of Stem Cell Research, Helmholtz Center, Munich, Germany. [2] Biomedical Center, University of Munich, Munich, Germany. [3] Munich cluster for Systems Neurology (SYNERGY), Ludwig-Maximilians-Universität, München, Germany.
Jovica
J
Ninkovic
D000818:Animals; D019070:Cell Lineage / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D002999:Clone Cells / Q000502:physiology; D004805:Ependyma; D020547:Lateral Ventricles / Q000166:cytology*; D051379:Mice; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology; D013194:Staining and Labeling