Two dimensional separations of human urinary protein digest using a droplet-interfaced platform.
For highly complex mixtures, coelution is a common phenomenon in chromatography. A great deal of resolution is hidden in coelution, and lost due to inevitable molecular diffusion during sample transfer. The molecular diffusion may lead to band broadening and remix of separated peaks, which cause degradation of achievable resolution. In this study, we introduced droplet microfluidics as a high performance sample transfer tool in two dimensional nanoflow liquid chromatography-capillary electrophoresis separation of a human urine sample. The fine fractionation capability and sampling completeness enabled by the droplet-interface demonstrated the 2D system's usefulness in high-resolution mapping of real world biological samples.
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Linquan
L
Ye
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Xin
X
Wang
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Jing
J
Han
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Fan
F
Gao
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Lingjia
L
Xu
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Zhiliang
Z
Xiao
Department of Urology, Xiamen University Affiliated Zhongshan Hospital, Xiamen 361004, China.
Peiming
P
Bai
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Qiuquan
Q
Wang
Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: bozhang@xmu.edu.cn.
Bo
B
Zhang
D000328:Adult; D002853:Chromatography, Liquid / Q000295:instrumentation; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation; D010316:Particle Size; D011506:Proteins / Q000302:isolation & purification*; D013499:Surface Properties; D016482:Urinalysis / Q000295:instrumentation*
D011506:Proteins
Capillary electrophoresis; Liquid chromatography; Microfluidics; Multidimensional separation; Proteomics