Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells.
BACKGROUND
BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells.


RESULTS
The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells.


CONCLUSIONS
Our study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. leimiao.yin@gmail.com.
Lei-Miao
LM
Yin
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. zhenjiu94@126.com.
Ying
Y
Wei
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. wqwang1988@163.com.
Wen-Qian
WQ
Wang
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. wyshcn@139.com.
Yu
Y
Wang
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. xydong0303@126.com.
Yu-Dong
YD
Xu
Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030, China. yyq@shutcm.edu.cn.
Yong-Qing
YQ
Yang
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D040502:Calgranulin B / Q000008:administration & dosage; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D061251:Primary Cell Culture; D051381:Rats; D011933:Reagent Kits, Diagnostic; D013673:Technology Assessment, Biomedical / Q000379:methods*; D013778:Tetrazolium Salts / Q000494:pharmacology*; D014132:Trachea / Q000166:cytology* / Q000254:growth & development
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D040502:Calgranulin B; D011933:Reagent Kits, Diagnostic; D013778:Tetrazolium Salts; D001973:Bromodeoxyuridine