Biological Activities of the Polysaccharides Produced from Different Sources of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus.
Xylaria nigripes, a local rare medicinal fungus, has multi-antioxidant activities owing to its water extraction as shown by previous research. However, the main indicator causing the antioxidant effect was not clear, so this research focused on the antioxidant activities from different sources of X. nigripes such as fruiting body polysaccharides, mycelium intracellular polysaccharides, mycelium extracellular polysaccharides, and their deproteinization products. The mycelium intracellular polysaccharide (XnIPS-1) from X. nigripes showed the highest reducing power of antioxidant activity, since it revealed the lowest IC50 values in all the assayed methodologies. The IC50 values of chelating ferrous ion ability, ABTS radical scavenging activity, and DPPH free radical scavenging were 1412, 174.25, and 351.56 µg/mL, respectively. In addition to these results, this research also explored the mechanism between polysaccharides and antioxidants compared by FT-IR analysis. The spectrum shows that the X. nigripes polysaccharide structure changed after the proteins were removed.
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Mu-Chun
MC
Hung
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Chia-Chun
CC
Tsai
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.); Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Tai-Hao
TH
Hsu
Department of Bioresources, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Zeng-Chin
ZC
Liang
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan.
Fang-Yi
FY
Lin
School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan.
Shih-Liang
SL
Chang
Department of Medicinal Botanicals and Healthcare, Department of Bioresources, Da-Yeh University, Datsun, Changhua, 51591 Taiwan.
Wai-Jane
WJ
Ho
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Chang-Wei
CW
Hsieh
D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles / Q000378:metabolism; D001688:Biological Products / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D056229:Fungal Structures / Q000737:chemistry; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry*; D010851:Picrates / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism; D013098:Xylariales / Q000737:chemistry*
D000975:Antioxidants; D052160:Benzothiazoles; D001688:Biological Products; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D062610:Fungal Polysaccharides; D010851:Picrates; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl