[Seroprevalance of Toxoplasma gondii infection and genotyping of the isolates from cancer patients in Anhui, eastern China].
OBJECTIVE
To investigate the seroprevalence of Toxoplasma gondii infection and identify the genotypes of T. gondii isolates from cancer patients in Anhui Province.


METHODS
Three hundred and fifty-six blood samples were collected from outpatients and hospitalized cancer patients in Hefei, Anhui Province. IgG and gM antibodies specific to T gondii were determined by ELISA. The ELISA positive samples were subjected to detection of Toxoplasma DNA with PCR targeting a 529-bp repeat element of T. gondii. Genotyping of T. gondii isolates was performed using multilocus PCR-RFLP and 10 genetic markers, including 9 nuclear loci, sagl, sag2, sag3, btub, gra6, L358, pkl, c22-8, c29-2, and apico.


RESULTS
Among 356 cancer patients, 21 (5.9%) cases were found to be IgG-positive and 8 (2.3%) were IgM-positive, and five of them were found to have both IgG and IgM antibodies. The total seroprevalence of Toxoplasma infection was 6.8%. Six PCR-positive samples were genotyped at 10 loci and two of them obtained all genetic markers and identified as the genotype of Chinese 1 (ToxoDB#9).


CONCLUSIONS
In this study, latent and active toxoplasmosis exist in the patients with malignant tumors, and two isolates are genotyped as the type of Chinese 1 (ToxoDB#9) in Anhui, China.
Qian
Q
Shen
Lin
L
Wang
Qiang
Q
Fang
Ji-long
JL
Shen
Lin-jie
LJ
Zhang
D000818:Animals; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D009369:Neoplasms / Q000150:complications*; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics*; D014123:Toxoplasmosis / Q000097:blood / Q000150:complications / Q000453:epidemiology*
D005819:Genetic Markers