Regulation of NF-κB signaling in osteoclasts and myeloid progenitors.
The transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is crucial for immune responses and skeletal development. Work in recent years has shown that various members of the NF-κB family are viable targets to regulate activity and survival of bone cells and hence bone metabolism. In this regard, deletion of upstream kinases or distal NF-κB subunits resulted with bone deformities. Thus, it has become increasingly apparent that detailed investigation of NF-κB in bone cells may provide opportunities to design new therapeutic modalities. In this chapter we present modified methodology describing efficient approaches to regulate the NF-κB pathway in vitro and in vivo to assess its function in bone cells and tissues.
Department of Orthopedic Surgery-Research, Washington University School of Medicine, 660 S. Euclid Ave, Campus Box 8233, Saint Louis, MO, 63110, USA.
Gaurav
G
Swarnkar
Yousef
Y
Abu-Amer
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D001862:Bone Resorption / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D051550:I-kappa B Kinase / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D023461:Myeloid Progenitor Cells / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D011485:Protein Binding; D015398:Signal Transduction / Q000187:drug effects; D058207:Sumoylation
D047908:Intracellular Signaling Peptides and Proteins; C467063:NBD peptide, mouse; C514981:NEMO protein, mouse; D016328:NF-kappa B; D010455:Peptides; D051550:I-kappa B Kinase