H2S analysis in biological samples using gas chromatography with sulfur chemiluminescence detection.
Hydrogen sulfide (H2S) is a metabolite and signaling molecule in biological tissues that regulates many physiological processes. Reliable and sensitive methods for H2S analysis are necessary for a better understanding of H2S biology and for the pharmacological modulation of H2S levels in vivo. In this chapter, we describe the use of gas chromatography coupled to sulfur chemiluminescence detection to measure the rates of H2S production and degradation by tissue homogenates at physiologically relevant concentrations of substrates. This method allows separation of H2S from other sulfur compounds and provides sensitivity of detection to ~15 pg (or 0.5 pmol) of H2S per injected sample.
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Victor
V
Vitvitsky
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA. Electronic address: rbanerje@umich.edu.
Ruma
R
Banerjee
D000818:Animals; D002849:Chromatography, Gas / Q000592:standards; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008163:Luminescent Measurements / Q000592:standards; D012015:Reference Standards
D006862:Hydrogen Sulfide
Biological samples; Gas chromatography; Hydrogen sulfide; Sulfur chemiluminescence detection