Morphological, Molecular, and Biochemical Characterization of Monounsaturated Fatty Acids-Rich Chlamydomonas sp. KIOST-1 Isolated from Korea.
Microalgae hold promise as producers of sustainable biomass for the production of biofuels and other biomaterials. However, the selection of strains with efficient and robust production of desirable resources remains challenging. In this study, we isolated a green microalga from Korea and analyzed its morphological, molecular, and biochemical characteristics. Microscopic and phylogenetic analyses demonstrated that the isolate could be classified into the genus Chlamydomonas, and we designated the isolate Chlamydomonas s p. K IOST -1. Compositions of protein, lipid, and carbohydrate in the microalgal cells were estimated to be 58.8 ± 0.2%, 22.7 ± 1.2%, and 18.5 ± 1.0%, respectively. Similar to other microalgae belonging to Chlorophyceae, the dominant amino acid and monosaccharide in Chlamydomonas sp. KIOST-1 were glutamic acid and glucose. On the other hand, the proportions of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids clearly differed from other species in the genus Chlamydomonas, and monounsaturated fatty acids accounted for a large portion (41.3%) of the total fatty acids in the isolate. Based on these results, Chlamydomonas sp. KIOST-1 has advantageous characteristics for biomass production.
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Seon-Mi
SM
Jeon
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.|Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 404-170, Republic of Korea.
Ji Hyung
JH
Kim
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Taeho
T
Kim
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Areumi
A
Park
Deep-sea and Seabed Resources Research Division, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Ah-Ra
AR
Ko
Deep-sea and Seabed Resources Research Division, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Se-Jong
SJ
Ju
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Soo-Jin
SJ
Heo
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Chulhong
C
Oh
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.|Departments of Marine Biology, Faculty of Marine Science, King AbdulAziz University, Jeddah 21589, Saudi Arabia.
Md Abu
MA
Affan
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.|Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843-2132, USA.
Won-Bo
WB
Shim
Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Do-Hyung
DH
Kang
D018533:Biomass; D002696:Chlamydomonas / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis / Q000737:chemistry*; D056910:Republic of Korea
D005229:Fatty Acids, Monounsaturated