Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans.
The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.
Department of Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Sang Duck
SD
Jeon
Department of Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Su Jung
SJ
Kim
Department of Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Sung Hyun
SH
Park
Changhae Advanced Institute of Technology, Changhae Ethanol Co., Ltd., Jeonju 561-203, Republic of Korea.
Gi-Wook
GW
Choi
Department of Biotechnology, Korea University, Seoul 136-701, Republic of Korea. Electronic address: samhan@korea.ac.kr.
Sung Ok
SO
Han
D001426:Bacterial Proteins / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D046974:Clostridium cellulovorans / Q000378:metabolism*; D006868:Hydrolysis
D001426:Bacterial Proteins; D002482:Cellulose
Cellulose hydrolysis; Cellulosome; Clostridium cellulovorans; Scaffolding protein; Synergistic effect