Trading polymeric microspheres: exchanging DNA molecules via microsphere interaction.
A new class of artificial molecular transport system is constructed by polymeric microspheres. The microspheres are prepared by self-assembly of poly(ethylene glycol)-block-poly(3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate), PEG-b-PDMAPS, by intermolecular dipole-dipole interaction of sulfobetaine side chains in water. Below the upper critical solution temperature (UCST) of PEG-b-PDMAPS, the microspheres (∼1μm) interact with other microspheres by partial and transit fusion. In order to apply the interaction between microspheres, a 3'-TAMRA-labeled single-stranded DNA oligomer (ssDNA) is encapsulated into a PEG-b-PDMAPS microsphere by thermal treatment. The exchange of ssDNA between microspheres is confirmed by fluorescence resonance energy transfer (FRET) quenching derived from double-stranded formation with complementary 5'-BHQ-2-labeled ssDNA encapsulated in PEG-b-PDMAPS microspheres. The exchange rate of ssDNA is controllable by tuning the composition of the polymer. The contact-dependent transport of molecules can be applied in the areas of microreactors, sensor devices, etc.
Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan. Electronic address: morimoto@material.tohoku.ac.jp.
Nobuyuki
N
Morimoto
Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Kanna
K
Muramatsu
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Shin-Ichiro M
SM
Nomura
Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Makoto
M
Suzuki
D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004339:Drug Compounding; D055459:Facilitated Diffusion; D031541:Fluorescence Resonance Energy Transfer; D006358:Hot Temperature; D008689:Methacrylates / Q000737:chemistry*; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D014867:Water
C530265:3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate; D015342:DNA Probes; D004277:DNA, Single-Stranded; D008689:Methacrylates; D000644:Quaternary Ammonium Compounds; D014867:Water; D011092:Polyethylene Glycols
Double strand formation; Microspheres; Self-assembly; Single-stranded DNA oligomer; Zwitterionic block copolymers