Asymmetric biodegradable microdevices for cell-borne drug delivery.
Use of live cells as carriers for drug-laden particulate structures possesses unique advantages for drug delivery. In this work, we report on the development of a novel type of particulate structures called microdevices for cell-borne drug delivery. The microdevices were fabricated by soft lithography with a disklike shape. Each microdevice was composed of a layer of biodegradable thermoplastic such as poly(lactic-co-glycolic acid). One face of the thermoplastic layer was covalently grafted with a cell-adhesive polyelectrolyte such as poly-l-lysine. This asymmetric structure allowed the microdevices to bind to live cells through bulk mixing without causing cell aggregation. Moreover, the cell-microdevice complexes were largely stable, and the viability and proliferation ability of the cells were not affected by the microdevices over a week. In addition, sustained release of a mock drug from the microdevices was demonstrated. This type of microdevice promises to be clinically useful for sustained intravascular drug delivery.
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.
Junfei
J
Xia
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.
Zhibin
Z
Wang
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.
Danting
D
Huang
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.
Yuanwei
Y
Yan
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.
Yan
Y
Li
†Department of Chemical and Biomedical Engineering FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310-2870, United States.|‡Integrative NanoScience Institute, Florida State University, Tallahassee, Florida 32306-4370, United States.
Jingjiao
J
Guan
D020341:Absorbable Implants; D017690:Cell Transplantation / Q000295:instrumentation*; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry; D004343:Drug Implants / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008904:Miniaturization; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054457:Tissue Scaffolds
D003692:Delayed-Action Preparations; D004343:Drug Implants; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
PLGA; cell therapies; cell-mediated drug delivery; microcontact printing; microfabrication; microparticles