A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks.
A total of 350 ticks collected in Austria were analyzed for the presence of DNA sequences of B. miyamotoi. Three ticks gave positive results in a B. miyamotoi-specific nested PCR. Results were confirmed by sequencing the amplified glpQ gene from the positive samples. Moreover we developed a real-time PCR which unambiguously detected B. miyamotoi in all positive samples. Further genotyping of the samples found 100% identity of the 16S-23S intergenic spacer region with Swedish B. miyamotoi sequences. This is the first detection of the relapsing fever spirochete Borrelia miyamotoi in hard ticks in Austria. The results consolidate the picture of a European-wide distribution of B. miyamotoi and again underscore the need for clinical awareness to clarify possible involvement of this species in human disease.
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria. Electronic address: michael.a.reiter@meduniwien.ac.at.
Michael
M
Reiter
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Anna-Margarita
AM
Schötta
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Andreas
A
Müller
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Hannes
H
Stockinger
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Gerold
G
Stanek
D000818:Animals; D001317:Austria / Q000453:epidemiology; D001483:Base Sequence; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012061:Relapsing Fever / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D021901:DNA, Intergenic
Borrelia; Ixodes; Miyamotoi; PCR; Tick