Nebivolol to attenuate the effects of hyper-homocysteinaemia in rats.
OBJECTIVE
This study investigated the prophylactic effect of nebivolol against hyper-homocysteinaemia (hHcy) induced oxidative stress in brain, heart, liver and kidney tissues and histomorphometric changes in the thoracic aorta.


METHODS
Twenty-four adult male Wistar rats were divided into a control, nebivolol, hHcy and nebivolol+hHcy group. hHcy was induced by oral administration of L-methionine (1 g/kg/day) for 28 days. 10 mg/kg/day nebivolol was administered orally for 28 days. Malondialdehyde (MDA) and glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activities in the tissues were determined. The total cross-sectional area (TCSA), luminal cross-sectional area (LCSA) and intima-media thickness (IMT) were measured in the thoracic aorta.


RESULTS
Homocysteine (Hcy) levels were lower in the nebivolol+hHcy group than in the hHcy group. Nebivolol treatment significantly decreased high MDA levels in the brain, heart and liver tissues. The level of GSH was higher in the brain, heart and kidney tissues of the nebivolol+hHcy group (P<0.001). The activity of CAT increased only in the kidney tissue of the nebivolol+hHcy group (P<0.01), and the activity of SOD was significantly increased in all the tissues in this group. Increased TCSA and IMT in the nebivolol+hHcy group were significantly decreased after nebivolol administration. The LCSA was significantly higher in the hHcy group than the control group, probably due to outward vascular remodelling.


CONCLUSIONS
Nebivolol treatment may be useful in different clinical scenarios where hHcy affects physiopathological pathways.
Department of Cardiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey. Electronic address: cagdasakgullu@gmail.com.
Cagdas
C
Akgullu
Department of Cardiology, Aydın City Hospital, Aydin, Turkey.
Mustafa Ahmet
MA
Huyut
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
Murat
M
Boyacioglu
Department of Histology and Embryology, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
Ozay
O
Guleş
Department of Cardiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
Ufuk
U
Eryilmaz
Department of Cardiology, Aydın City Hospital, Aydin, Turkey.
Tolga
T
Hekim
Department of Cardiology, Ada Tıp Hospital, Sakarya, Turkey.
Emir
E
Dogan
Department of Cardiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
Cemil
C
Zencir
Department of Cardiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
Hasan
H
Güngör
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019610:Cytoprotection; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D020138:Hyperhomocysteinemia / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008715:Methionine; D009206:Myocardium / Q000378:metabolism; D000068577:Nebivolol / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D066253:Vascular Remodeling / Q000187:drug effects
D000975:Antioxidants; D015415:Biomarkers; D000068577:Nebivolol; D008315:Malondialdehyde; D008715:Methionine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
Aorta; Hyper-homocysteinaemia; Nebivolol; Oxidant/anti-oxidant parameters; Rats