Molecularly imprinted coated graphene oxide solid-phase extraction monolithic capillary column for selective extraction and sensitive determination of phloxine B in coffee bean.
A method was developed to sensitively determine phloxine B in coffee bean by molecularly imprinted polymers (MIPs) coated graphene oxide (GO) solid-phase extraction (GO-MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC-LIF). The GO-MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using GO as supporting material, phloxine B, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade GO-MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions. The GO-MIPs were characterized by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FT-IR). The mean recoveries of phloxine B in coffee bean ranged from 89.5% to 91.4% and the intra-day and inter-day relative standard deviation (RSD) values all ranged from 3.6% to 4.7%. Good linearity was obtained over 0.001-2.0 μg mL(-1) (r=0.9995) with the detection limit (S/N=3) of 0.075 ng mL(-1). Under the selected conditions, enrichment factors of over 90-fold were obtained and extraction on the monolithic column effectively cleaned up the coffee bean matrix. The results demonstrated that the proposed GO-MISPE HPLC-LIF method can be applied to sensitively determine phloxine B in coffee bean.
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: zhaihaiyun@126.com.
Haiyun
H
Zhai
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zihao
Z
Su
School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: chenzg@mail.sysu.edu.cn.
Zuanguang
Z
Chen
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhenping
Z
Liu
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Kaisong
K
Yuan
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Lu
L
Huang
D003069:Coffee / Q000737:chemistry*; D010697:Eosine I Bluish / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D054802:Molecular Imprinting; D010087:Oxides / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D052616:Solid Phase Extraction / Q000295:instrumentation*
D003069:Coffee; D010087:Oxides; D010936:Plant Extracts; D006108:Graphite; D010697:Eosine I Bluish
Graphene oxide-molecularly imprinted polymers; In situ polymerization; Monolithic capillary column; Phloxine B; Solid-phase extraction