Silylated precision particles for controlled release of proteins.
With the recent advances in the development of novel protein based therapeutics, controlled delivery of these biologics is an important area of research. Herein, we report the synthesis of microparticles from bovine serum albumin (BSA) as a model protein using Particle Replication in Non-wetting Templates (PRINT) with specific size and shape. These particles were functionalized at room temperature using multifunctional chlorosilane that cross-link the particles to render them to slowly-dissolving in aqueous media. Mass spectrometric study of the reaction products of diisopropyldichlorosilane with individual components of the particles revealed that they are capable of reacting and forming cross-links. Energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were also used to confirm the functionalization of the particles. Cross sectional analysis using focused ion beam (FIB) and EDS proved that the functionalization occurs throughout the bulk of the particles and is not just limited to the surface. Circular dichroism data confirmed that the fraction of BSA molecules released from the particles retains its secondary structure thereby indicating that the system can be used for delivering protein based formulations while controlling the dissolution kinetics.
†Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.
Khosrow
K
Khodabandehlou
Amar S
AS
Kumbhar
Sohrab
S
Habibi
Ashish A
AA
Pandya
J Christopher
JC
Luft
†Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.
Saad A
SA
Khan
†Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.|∇Sloan-Kettering Institute for Cancer Research, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, United States.
Joseph M
JM
DeSimone
D065966:Absorption, Physicochemical; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004058:Diffusion; D006859:Hydrogen / Q000737:chemistry; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D003692:Delayed-Action Preparations; D053769:Nanocapsules; D012821:Silanes; D012710:Serum Albumin, Bovine; D006859:Hydrogen; D012825:Silicon
bovine serum albumin; controlled release; diisopropyldichlorosilane; functionalization; protein delivery; silylation