RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane.
RhoD is a member of the Rho GTPase family and it coordinates actin dynamics and membrane trafficking. Activation of RhoD results in formation of filopodia, dissolution of stress fibers, and the subsequent formation of short actin bundles. In addition, RhoD localizes to early endosomes and recycling endosomes, and has a regulatory role in endosome trafficking. In this study, we report on a function of RhoD in the regulation of Golgi homeostasis. We show that manipulation of protein and activation levels of RhoD, as well as of its binding partner WHAMM, result in derailed localization of Golgi stacks. Moreover, vesicle trafficking from the endoplasmic reticulum to the plasma membrane via the Golgi apparatus measured by the VSV-G protein is severely hampered by manipulation of RhoD or WHAMM. In summary, our studies demonstrate a novel role for this member of the Rho GTPases in the regulation of Golgi function.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Magdalena
M
Blom
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Katarina
K
Reis
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Vishal
V
Nehru
Applied Physics, Royal Institute of Technology, Science for Life Laboratory, SE-171 21 Stockholm, Sweden.
Hans
H
Blom
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Annica K B
AKB
Gad
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden. Electronic address: pontus.aspenstrom@ki.se.
Pontus
P
Aspenström
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D055785:Gene Knockdown Techniques; D006056:Golgi Apparatus / Q000201:enzymology*; D006706:Homeostasis; D006801:Humans; D007425:Intracellular Membranes / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D021381:Protein Transport; D022161:Transport Vesicles / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000502:physiology*
D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C578123:WHAMM protein, human; C105243:RHOD protein, human; D020741:rho GTP-Binding Proteins
Golgi; Rho GTPases; RhoD; VSV-G; WHAMM