Preparation and separation of DNA-wrapped carbon nanotubes.
Purification of single-chirality single-wall carbon nanotubes (SWCNTs) from their synthetic mixture is a prerequisite for many applications. DNA-controlled carbon nanotube (CNT) purification has evolved over a decade along with other separation techniques utilizing different types of dispersing agents such as surfactants and polymers. The size of single-stranded DNA (ssDNA) libraries affords practically unlimited ways of coating SWCNTs. Recent developments in separating surfactant-dispersed SWCNTs by polymer aqueous two-phase (ATP) extraction has enabled rapid and efficient SWCNT separation on a larger volume scale. Applying the ATP separation method to DNA-SWCNT hybrids opens a new route for effective sorting of nanotubes into each and every single-chirality species. Here, we report protocols for purifying as many as 15 single-chirality nanotube species from a synthetic mixture based on the separation of DNA-SWCNTs by the aqueous two-phase (ATP) method.
Materials Science and Engineering Division, National Institute of Standards and Technology, Gaithersburg, Maryland.
Geyou
G
Ao
Ming
M
Zheng
D002623:Chemistry Techniques, Analytical; D004277:DNA, Single-Stranded / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000032:analysis / Q000737:chemistry; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry
D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D011108:Polymers; D013501:Surface-Active Agents
ATP system; DNA sequences; carbon nanotube purification; modulating agents; single-chirality