Photothermally induced local dissociation of collagens for harvesting of cell sheets.
The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by a photothermal effect directed by near-infrared (NIR) light induces unfolding of absorbed collagen triple helices, yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of a living idiomorphic cell sheet, achieved upon irradiation with NIR light (λ=808 nm). The PEDOT layer was patterned and cells were successfully cultured on the patterned substrate. Cell sheets of various shapes mirroring the PEDOT pattern could be detached after a few minutes of irradiation with NIR light. The PEDOT patterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets. This photothermally induced dissociation of collagen provided a fast non-invasive harvesting method and tailor-made cell-sheet patterns.
Active Polymer Center for Pattern Integration and Department of Chemical and Biomolecular Engineering, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul 120-749 (Republic of Korea).
Jae Dong
JD
Kim
June Seok
JS
Heo
Teahoon
T
Park
Chihyun
C
Park
Hyun Ok
HO
Kim
Eunkyoung
E
Kim
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002469:Cell Separation / Q000379:methods*; D003094:Collagen / Q000378:metabolism* / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007259:Infrared Rays; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry; D013696:Temperature
D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D003094:Collagen
collagen peptides; conducting materials; photothermal effect; polymers; protein folding