Applicability of the ParaDNA(®) Screening System to Seminal Samples.
Seminal fluid represents a common biological material recovered from sexual assault crime scenes. Such samples can be prescreened using different techniques to determine cell type and relative amount before submitting for full STR profiling. The ParaDNA(®) Screening System is a novel forensic test which identifies the presence of DNA through amplification and detection of two common STR loci (D16S539 and TH01) and the Amelogenin marker. The detection of the Y allele in samples could provide a useful tool in the triage and submission of sexual assault samples by enforcement authorities. Male template material was detected on a range of common sexual assault evidence items including cotton pillow cases, condoms, swab heads and glass surfaces and shows a detection limit of 1 in 1000 dilution of neat semen. These data indicate this technology has the potential to be a useful tool for the detection of male donor DNA in sexual assault casework.
LGC Forensics, Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, U.K.
Nicholas D
ND
Tribble
Jamie A D
JA
Miller
Nick
N
Dawnay
Nicola J
NJ
Duxbury
D000483:Alleles; D053523:Amelogenin / Q000235:genetics; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D015342:DNA Probes; D005453:Fluorescence; D005554:Forensic Medicine / Q000295:instrumentation*; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D012742:Sex Offenses; D012984:Software
D053523:Amelogenin; D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA
DNA; forensic science; semen; sexual violence; short tandem repeats; triage