Acute and subchronic (28 days) oral toxicity studies of Codonopsis lanceolata extract in Sprague-Dawley rats.
Codonopsis lanceolata is a perennial plant that has been used as a food and in traditional medicine for the treatment of cough, bronchitis, and inflammation in East Asia including Korea, Japan, and China. However, information regarding its toxicity is limited. Therefore, we performed a safety evaluation of aqueous C. lanceolata root extract (CLE) in Sprague-Dawley rats. Assessment of acute toxicity revealed that CLE did not influence mortality, clinical appearance, body weight gain, or necropsy findings at a dose of 5000 mg/kg body weight. In the subchronic oral toxicity, data revealed that several significant alteration in food consumption, water consumption, protein excretion, WBCs levels, TGs, BUN levels, and the absolute and relative weights in the liver, spleen and lungs. However, these changes were transient and were not considered treatment related because they showed no apparent dose dependent. These results suggest that CLE (1250, 2500, and 5000 mg/kg body weight/day) administered orally does not cause acute or subchronic toxicity to male or female rats. The 50% lethal dose (LD50) of CLE was determined to be greater than 5000 mg/kg.
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea; National Institute of Biological Resources, Incheon 404-708, South Korea.
Jong Seok
JS
Lee
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.
Young-Hyun
YH
Kim
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.
Dan-Bi
DB
Kim
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.
Gi-Hae
GH
Shin
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea.
Jin-Ha
JH
Lee
Hurum Central Research Institute, Ochang 363-883, South Korea.
Ju-Hyun
JH
Cho
Department of Food Science and Biotechnology, CHA University, Seongnam 463-836, South Korea.
Boo-Yong
BY
Lee
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address: loh99@kangwon.ac.kr.
Ok-Hwan
OH
Lee
D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D029745:Codonopsis; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018517:Plant Roots; D010946:Plants, Medicinal; D011507:Proteinuria / Q000139:chemically induced / Q000652:urine; D017207:Rats, Sprague-Dawley; D018570:Risk Assessment; D013997:Time Factors; D023382:Toxicity Tests, Acute / Q000379:methods; D036401:Toxicity Tests, Chronic / Q000379:methods
D015415:Biomarkers; D010936:Plant Extracts
Codonopsis lanceolata; Hematology; Histopathology; Serum biochemistry; Toxicity