Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus.
Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.
Faculty of Agronomy, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China. wangyuelin1989@163.com.|Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. wangyuelin1989@163.com.
Yun-Peng
YP
Wang
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. weizy@cjaas.com.
Zheng-Yi
ZY
Wei
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. yuying0609@126.com.|College of Biological Sciences, China Agricultural University, No. 2 West Yuanmingyuan Road, Beijing 100094, China. yuying0609@126.com.
Yu-Ying
YY
Zhang
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. lincj@cjaas.com.
Chun-Jing
CJ
Lin
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. zhongxf@cjaas.com.
Xiao-Fang
XF
Zhong
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. wangyuelin1989@163.com.
Yue-Lin
YL
Wang
Faculty of Agronomy, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China. 99n2@163.com.
Jing-Yong
JY
Ma
Faculty of Agronomy, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China. majian19790106@163.com.
Jian
J
Ma
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China. xingsc@cjaas.com.
Shao-Chen
SC
Xing
D000528:Alternaria / Q000187:drug effects / Q000502:physiology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D015153:Blotting, Western; D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects; D054884:Host-Pathogen Interactions / Q000187:drug effects; D020082:Magnaporthe / Q000502:physiology; D018613:Microscopy, Confocal; D012275:Oryza / Q000382:microbiology; D010455:Peptides / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000382:microbiology
D023181:Antimicrobial Cationic Peptides; C446524:MSI-99 peptide; D010455:Peptides; D011994:Recombinant Proteins