A dual-responsive pH-sensor and its potential as a universal probe for assays of pH-changing enzymes.
We described a dual turn-on probe sensitive to both acidity and basicity, which could be designed by connecting a fluorophore to a quencher via metal-ligand interaction. Atto488-labeled nitrilotriacetic acid and polyhistidine peptide were used as the fluorophore and the quencher, respectively, and linked to each other by coordination with a cobalt(II) ion. After preparation of the probe, the pH-sensitive dual turn-on property of the probe has been successfully observed upon responding to both acidity and basicity of the solution. The probe has been employed as a signal reporter in assays of pH-changing enzymes such as penicillinase generating acidity and adenosine deaminase generating basicity. Furthermore, the practical utility of the probe was also demonstrated by utilizing the probe in the discrimination of β-lactamase-producing bacteria.
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. drahn@kist.re.kr.
Hien Bao Dieu
HB
Thai
Jin Kyung
JK
Yu
Yeon-Joon
YJ
Park
Dae-Ro
DR
Ahn
D001419:Bacteria / Q000201:enzymology; D003035:Cobalt / Q000737:chemistry; D057075:Enzyme Assays / Q000379:methods*; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D001618:beta-Lactamases / Q000378:metabolism*
C033223:polyhistidine; D003035:Cobalt; D006639:Histidine; D001618:beta-Lactamases; D009571:Nitrilotriacetic Acid