Hydrophilic PCU scaffolds prepared by grafting PEGMA and immobilizing gelatin to enhance cell adhesion and proliferation.
Gelatin contains many functional motifs which can modulate cell specific adhesion, so we modified polycarbonate urethane (PCU) scaffold surface by immobilization of gelatin. PCU-g-gelatin scaffolds were prepared by direct immobilizing gelatins onto the surface of aminated PCU scaffolds. To increase the immobilization amount of gelatin, poly(ethylene glycol) methacrylate (PEGMA) was grafted onto PCU scaffolds by surface initiated atom transfer radical polymerization. Then, following amination and immobilization, PCU-g-PEGMA-g-gelatin scaffolds were obtained. Both modified scaffolds were characterized by chemical and biological methods. After immobilization of gelatin, the microfiber surface became rough, but the original morphology of scaffolds was maintained successfully. PCU-g-PEGMA-g-gelatin scaffolds were more hydrophilic than PCU-g-gelatin scaffolds. Because hydrophilic PEGMA and gelatin were grafted and immobilized onto the surface, the PCU-g-PEGMA-g-gelatin scaffolds showed low platelet adhesion, perfect anti-hemolytic activity and excellent cell growth and proliferation capacity. It could be envisioned that PCU-g-PEGMA-g-gelatin scaffolds might have potential applications in tissue engineering artificial scaffolds.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Changcan
C
Shi
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Wenjie
W
Yuan
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Musammir
M
Khan
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Qian
Q
Li
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China; Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin) Tianjin 300072, China. Electronic address: yakaifeng@tju.edu.cn.
Yakai
Y
Feng
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China; Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin 300072, China.
Fanglian
F
Yao
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China. Electronic address: wenchengzhang@yahoo.com.
Wencheng
W
Zhang
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005780:Gelatin / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000494:pharmacology*; D008689:Methacrylates / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011140:Polyurethanes / Q000494:pharmacology*; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water
D055767:Immobilized Proteins; D008689:Methacrylates; D011140:Polyurethanes; C524499:polyethylene glycol methacrylate; D014867:Water; D011092:Polyethylene Glycols; D005780:Gelatin
Gelatin; Immobilization; PEGMA; Polycarbonate urethanes; Scaffolds