Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.
The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A (125)I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Anna
A
Golunova
‡Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Řež 130, 250 68 Řež, Czech Republic.
David
D
Chvátil
‡Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Řež 130, 250 68 Řež, Czech Republic.
Pavel
P
Krist
§Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Josef
J
Jaroš
§Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Veronika
V
Jurtíková
§Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Jakub
J
Pospíšil
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Ilya
I
Kotelnikov
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Lucie
L
Abelová
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Jiří
J
Kotek
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Tomáš
T
Sedlačík
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.|‡Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Řež 130, 250 68 Řež, Czech Republic.
Jan
J
Kučka
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Jana
J
Koubková
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Hana
H
Studenovská
∥Department of Plastic and Aesthetic Surgery, St. Anne University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
Libor
L
Streit
§Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Aleš
A
Hampl
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
František
F
Rypáček
†Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06 Prague 6, Czech Republic.
Vladimír
V
Proks
D017667:Adipocytes / Q000187:drug effects; D059985:Cryogels / Q000138:chemical synthesis* / Q000494:pharmacology; D004583:Electrons; D006801:Humans; D058105:Polymerization; D016062:Porosity
D059985:Cryogels