The effect of Fe2O3 and ZnO nanoparticles on cytotoxicity and glucose metabolism in lung epithelial cells.
Metallic nanoparticles (NPs) have potential applications in industry and medicine, but they also have the potential to cause many chronic pulmonary diseases. Mechanisms for their cytotoxicity, glucose and energy metabolism responses need to be fully explained in lung epithelial cells after treatment with metallic nanoparticles. In our study, two different metallic nanoparticles (Fe2 O3 and ZnO) and two cell-based assays (BEAS-2B and A549 cell lines) were used. Our findings demonstrate that ZnO nanoparticles, but not Fe2 O3 nanoparticles, induce cell cycle arrest, cell apoptosis, reactive oxygen species (ROS) production, mitochondrial dysfunction and glucose metabolism perturbation, which are responsible for cytotoxicity. These results also suggest that the glucose metabolism and bioenergetics had a great potential in evaluating the cytotoxicity and thus were very helpful in understanding their underlying molecular mechanisms.
State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, the Fourth Military Medical University, 710032, Xi'an, China.
Xiaofeng
X
Lai
Yifang
Y
Wei
Hu
H
Zhao
Suning
S
Chen
Xin
X
Bu
Fan
F
Lu
Dingding
D
Qu
Libo
L
Yao
Jianyong
J
Zheng
Jian
J
Zhang
D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D005290:Ferric Compounds / Q000633:toxicity*; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020545:Respiratory Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D015034:Zinc Oxide / Q000633:toxicity*
D005290:Ferric Compounds; D017382:Reactive Oxygen Species; C000499:ferric oxide; D005947:Glucose; D015034:Zinc Oxide
ROS; cell cycle arrest; cytotoxicity; glucose metabolism; mitochondrial dysfunction; nanoparticles