Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103.
This study investigated the mechanism of arsenic resistance in the diazotrophic bacterium Herbaspirillum sp. GW103 isolated from rhizosphere soil of Phragmites austrails. The isolate Herbaspirillum sp. GW103 exhibited maximum tolerance to arsenic (550 mg/L). Four different arsenate reductase (arsC) genes (arsC1, arsC2, arsC3 and arsC4) were located in the genome of the isolate Herbaspirillum sp. GW103. The expression pattern of the arsC1 differed from other genes. All four types of arsC genes had different protein secondary structures and stereochemical properties. Molecular modeling and structural analysis of arsC genes revealed close structural homology with arsC family proteins from Escherichia coli (PDB ID: 1I9D) and Pseudomonas aeruginosa (PDB ID: 1RW1).
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
Muthusamy
M
Govarthanan
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea.
Sang-Myeong
SM
Lee
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea. Electronic address: kannan@jbnu.ac.kr.
Seralathan
S
Kamala-Kannan
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, South Korea. Electronic address: btoh@jbnu.ac.kr.
Byung-Taek
BT
Oh
D000595:Amino Acid Sequence; D053502:Arsenate Reductases / Q000737:chemistry / Q000235:genetics*; D001151:Arsenic / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D042803:Herbaspirillum / Q000201:enzymology* / Q000235:genetics*; D019216:Metals, Heavy; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D058441:Rhizosphere; D012988:Soil Microbiology
D001426:Bacterial Proteins; D019216:Metals, Heavy; D053502:Arsenate Reductases; D001151:Arsenic
Arsenate reductase; Heavy metals; Herbaspirillum sp.; Real-time PCR