Common isolation of New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae in a large surgical hospital in Vietnam.
This study sought to monitor the presence of carbapenem-resistant Enterobacteriaceae (CRE) and the proportion New Delhi metallo-beta-lactamase 1 (NDM-1)-producing bacteria between August 2010 and December 2012 in a surgical hospital in Vietnam. We identified 47 CRE strains from a total of 4,096 Enterobacteriaceae isolates (1.1 %) that were NDM-1-positive from 45 patients admitted to 11 different departments, with the majority being from the urology department. The NDM-1 gene was found in seven different species. Genotyping revealed limited clonality of NDM-1-positive isolates. Most of the isolates carried the NDM-1 gene on a plasmid and 17.8 % (8/45) of those were readily transferable. We found five patients at admission and one patient at discharge with NDM-1-positive bacteria in their stool. From 200 screening environmental hospital samples, five were confirmed to be NDM-1-positive and included Acinetobacter species (n = 3) and Enterobacter aerogenes (n = 2). The results reveal that NDM-1-producing Enterobacteriaceae are commonly isolated in patients admitted to a Vietnamese surgical hospital and are also detected in the hospital environment.
National Institute of Hygiene and Epidemiology, Yersin Street 1, Hanoi, Vietnam, Trhuyhoang1791@gmail.com.
H H
HH
Tran
S
S
Ehsani
K
K
Shibayama
M
M
Matsui
S
S
Suzuki
M B
MB
Nguyen
D N
DN
Tran
V P
VP
Tran
D L
DL
Tran
H T
HT
Nguyen
D A
DA
Dang
H S
HS
Trinh
T H
TH
Nguyen
H F L
HF
Wertheim
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology*; D004783:Environmental Microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing; D010957:Plasmids / Q000032:analysis; D014744:Vietnam / Q000453:epidemiology; D055815:Young Adult; D001618:beta-Lactamases / Q000378:metabolism*
D001618:beta-Lactamases; C552864:beta-lactamase NDM-1