[Polymorphism analysis of Plasmodium falciparum histidine-rich protein II and III].
OBJECTIVE
To analyze the polymorphism of Plasmodium falciparum histidine-rich protein (PfHRP) II and III.


METHODS
Genomic DNA was isolated from blood samples of 20 patients infected with Plasmodium falciparum in Yunnan Province. Blood samples were tested by microscopy and RDTs. The Pfhrp2 and Pfhrp3 gene fragments were amplified by PCR and sequenced. The sequencing results were analyzed and compared using the bioinformatics software.


RESULTS
20 patients infected with Plasmodium falciparum tested by microscopy and RDTs. PCR showed that the Pfhrp2 gene was with 389~986 bp, and Pfhrp3 gene with 329-640 bp. All PfH-IRP II sequences started with type 1 repeat (AHHAHHVAD) and ended with the type 12 repeat (AHHAAAHHEAATH). The number of type 7 (AHHAAD), type 2 (AHHAHHAAD) and type 6 (AHHATD) within PfHRP II was more than the other types of repeats, as well as type 16 (AHHAAN) and type 17 (AHHDG) for PfHRP III. Type 11 repeat (AHN) was not found from the PfHRP II and PfHRP III sequences.


CONCLUSIONS
There is an extensive diversity in Pfhrp2 and Pfhrp3 fragments in the individuals infected with P. falciparum in Yunnan. Some types of repeats are shared by PfHRP II and PfHRP III.
Ying-chao
YC
Yang
Jin
J
Zhang
Guo-zhu
GZ
Wang
Shu-ying
SY
Bo
Ying
Y
Zhang
Xiao-fang
XF
Xin
D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D010455:Peptides / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*
D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D010455:Peptides; D015800:Protozoan Proteins; C042968:knob protein, Plasmodium falciparum