Hybridisation potential of 1',3'-Di-O-methylaltropyranoside nucleic acids.
In further study of our series of six-membered ring-containing nucleic acids, different 1',3'-di-O-methyl altropyranoside nucleoside analogs (DMANA) were synthesized comprising all four base moieties, adenine, cytosine, uracil and guanine. Following assembly into oligonucleotides (ONs), their affinity for natural oligonucleotides was evaluated by thermal denaturation of the respective duplexes. Data were compared with results obtained previously for both anhydrohexitol (HNAs) and 3'-O-methylated altrohexitol modified ONs (MANAs). We hereby demonstrate that ONs modified with DMANA monomers, unlike some of our previously described analogues with constrained 6-membered hexitol rings, did not improve thermodynamic stability of dsRNA complexes, most probably in view of an energetic penalty when forced in the required 1C4 pairing conformation. Overall, a single incorporation was more or less tolerated or even positive for the adenine congener, but incorporation of a second modification afforded a slight destabilization (except for A), while a fully modified sequence displayed a thermal stability of -0.3 °C per modification. The selectivity of pairing remained very high, and the new modification upon incorporation into a DNA strand, strongly destabilized the corresponding DNA duplexes. Unfortunately, this new modification does not bring any advantage to be further evaluated for antisense or siRNA applications.
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. akkaladevi.venkatesham@rega.kuleuven.be.
Akkaladevi
A
Venkatesham
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. ddkachare@gmail.com.
Dhuldeo
D
Kachare
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. Guy.Schepers@rega.kuleuven.be.
Guy
G
Schepers
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. Jef.Rozenski@rega.kuleuven.be.
Jef
J
Rozenski
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. Mathy.Froeyen@rega.kuleuven.be.
Mathy
M
Froeyen
Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, Leuven BE-3000, Belgium. Arthur.Vanaerschot@rega.kuleuven.be.
Arthur
A
Van Aerschot
D000225:Adenine / Q000737:chemistry; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics; D006147:Guanine / Q000737:chemistry; D009691:Nucleic Acid Denaturation / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics; D013402:Sugar Alcohols / Q000737:chemistry; D013816:Thermodynamics; D014498:Uracil / Q000737:chemistry
D009696:Nucleic Acids; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D013402:Sugar Alcohols; C543192:hexitol; D014498:Uracil; D006147:Guanine; D003596:Cytosine; D004247:DNA; D000225:Adenine