Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence.
The satellite cells, which serve as adult muscle stem cells, are both located beneath myofiber basement membranes and closely associated with capillary endothelial cells. We observed that 90% of capillaries were associated with pericytes in adult mouse and human muscle. During post-natal growth, newly formed vessels with their neuroglial 2 proteoglycan (NG2)-positive pericytes became progressively associated with the post-natal muscle stem cells, as myofibers increased in size and satellite cells entered into quiescence. In vitro, human muscle-derived pericytes promoted myogenic cell differentiation through insulin-like growth factor 1 (IGF1) and myogenic cell quiescence through angiopoietin 1 (ANGPT1). Diphtheria toxin-induced ablation of muscle pericytes in growing mice led both to myofiber hypotrophy and to impaired establishment of stem cells quiescence. Similar effects were observed following conditional in vivo deletion of pericyte Igf1 and Angpt1 genes, respectively. Our data therefore demonstrate that, by promoting post-natal myogenesis and stem cell quiescence, pericytes play a key role in the microvascular niche of satellite cells.
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est, 5 boulevard Descartes, Marne-la-Vallée cedex 2 F-77454, France.
Enis
E
Kostallari
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est Créteil, 62 avenue du Général de Gaulle, Créteil F-94000, France.
Yasmine
Y
Baba-Amer
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est Créteil, 62 avenue du Général de Gaulle, Créteil F-94000, France.
Sonia
S
Alonso-Martin
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université d'Evry-Val d'Essonne, Boulevard François Mitterrand, Evry F-91000, France.
Pamela
P
Ngoh
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est Créteil, 62 avenue du Général de Gaulle, Créteil F-94000, France Etablissement Français du Sang, Créteil 94017, France Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort 94700, France Hôpital Henri Mondor, Département de Pathologie, 51 avenue du Maréchal de Lattre de Tassigny, Créteil F-94010, France.
Frederic
F
Relaix
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est Créteil, 62 avenue du Général de Gaulle, Créteil F-94000, France.
Peggy
P
Lafuste
Institut Mondor de Recherche Biomédicale, INSERM U955-E10, Faculté de Médecine, 8 rue du Général Sarrail, Créteil F-94010, France Université Paris-Est Créteil, 62 avenue du Général de Gaulle, Créteil F-94000, France Hôpital Henri Mondor, Département de Pathologie, 51 avenue du Maréchal de Lattre de Tassigny, Créteil F-94010, France romain.gherardi@hmn.aphp.fr.
Romain K
RK
Gherardi
D000293:Adolescent; D042683:Angiopoietin-1 / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D042783:Endothelial Cells / Q000166:cytology; D017353:Gene Deletion; D006801:Humans; D007223:Infant; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008810:Mice, Inbred C57BL; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D020286:Pericytes / Q000166:cytology* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D055815:Young Adult
D042683:Angiopoietin-1; C467628:Angpt1 protein, mouse; D011956:Receptors, Cell Surface; D007334:Insulin-Like Growth Factor I
Human; Mouse; Pericytes; Post-natal development; Satellite cell differentiation; Satellite cell quiescence; Skeletal muscle