Mussel-inspired surface modification of magnetic@graphite nanosheets composite for efficient Candida rugosa lipase immobilization.
By the facile adhesion way, the novel composite complex by polydopamine (PDA) and magnetic graphite nanosheets (Fe3O4@GNSs) has been successfully synthesized. The resulting composite was characterized by means of scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, and Raman spectra, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Meanwhile, the PDA functionalized Fe3O4@GNSs (Fe3O4@GNSs-PDA) was applied for Candida rugosa lipase (CRL) immobilization covalently without any toxic coupling agent. Combining the superior physical properties and chemical stability of Fe3O4@GNSs and the well biocompatibility, functional characteristics of PDA, the Fe3O4@GNSs-PDA composite displayed several advantages, including the high enzyme capacity, enzyme activity and stability and a decrease in enzyme loss. Our work demonstrated that the mussel-inspired Fe3O4@GNSs can be extended to many other applications such as biocatalytic, genetic and industrial.
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, Lanzhou, 730000, China.
Chen
C
Hou
Lincheng
L
Zhou
Hao
H
Zhu
Xinyu
X
Wang
Niran
N
Hu
Fang
F
Zeng
Liyuan
L
Wang
Hang
H
Yin
D000268:Adhesiveness; D000818:Animals; D032701:Biomimetics; D001709:Biotechnology; D049872:Bivalvia; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D060328:Magnetic Phenomena; D008280:Magnetics; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
D004800:Enzymes, Immobilized; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D006108:Graphite; D008049:Lipase