Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria.
Bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. This pathology is at least partially caused by Anaplasma phagocytophilum, Anaplasma marginale, Anaplasma centrale, and Anaplasma bovis. The global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. In this study, we developed a new qPCR tool targeting the 23S rRNA gene for the detection of Anaplasmataceae bacteria. The primers and probe for the qPCR reaction had 100% specificity and could identify at least A. phagocytophilum, A. marginale, A. centrale, Anaplasma ovis, Anaplasma platys, Ehrlichia canis, Ehrlichia ruminantium, Neorickettisa sennetsu, and Neorickettsia risticii. We used this tool to test samples of bovines from Batna (Algeria), an area from which bovine anaplasmosis have never been reported. We identified three genetic variants of A. phagocytophilum, A. platys and Anaplasma sp. "variant 4". This finding should attract the attention of public authorities to assess the involvement of these pathogens in human and animal health.
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Aix-Marseille Université, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
Mustapha
M
Dahmani
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Aix-Marseille Université, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
Bernard
B
Davoust
Clinique vétérinaire Le Refuge, Batna, Algeria.
Mohamed Seghir
MS
Benterki
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Aix-Marseille Université, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France; Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Institut de Recherche pour le Développement, Centre IRD Hann, BP 1386, Dakar, Senegal.
Florence
F
Fenollar
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Aix-Marseille Université, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France; Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Institut de Recherche pour le Développement, Centre IRD Hann, BP 1386, Dakar, Senegal.
Didier
D
Raoult
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Aix-Marseille Université, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France; Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198 INSERM 1095, Institut de Recherche pour le Développement, Centre IRD Hann, BP 1386, Dakar, Senegal. Electronic address: olegusss1@gmail.com.
Oleg
O
Mediannikov
D000462:Algeria; D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification; D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification*; D000710:Anaplasmataceae / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial
23S rRNA gene; Algeria; Anaplasma phagocytophilum; Anaplasma platys; Anaplasmataceae; Cattle; PCR assay