Simple and rapid separation of soyasaponin Bb from a soy extract.
A simple method to separate soyasaponin Bb from a soy extract is presented. This method is based on the difference in the solubility of soyasaponin Bb and Ba and other components into 3:7 and 1:1 (v/v) acetone-water mixed solvents. The crude soyasaponin consisting of soyasaponins Aa, Ab, Ba, and Bb at the 10 wt% level and other components was examined as the soy extract. A 10 mg quantity of the crude soyasaponin was mixed with 1 mL of the 3:7 acetone-water containing 0.1 mol/L HCl, and the supernatant was removed to obtain a precipitate, which was found to contain mainly soyasaponins Bb and Ba. The precipitate was mixed with 0.4 mL of the 1:1 acetone-water containing 0.1 mol/L HCl; the supernatant was transferred, and was mixed with 0.6 mL of water to obtain a precipitate, which was found to contain mainly soyasaponin Bb. The yield was ca. 30%, which may be much higher than that by the conventional preparative chromatographic approach. The separation method is rapid and easy to carry out, and is useful for the preparation of a soyasaponin Bb sample.
Department of Bioscience, Fukui Prefectural University.
Hajime
H
Katano
Nobuhiro
N
Okamoto
Masahiro
M
Takakuwa
Shu
S
Taira
Taiho
T
Kambe
Masakazu
M
Takahashi
D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D010936:Plant Extracts / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification*; D013025:Glycine max / Q000737:chemistry*; D013997:Time Factors
D010936:Plant Extracts; D012503:Saponins; C000602851:soyasaponin Bb