Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus.
The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish that inhabits the beach rocky bottom from Japan to the South China Sea. Eleven polymorphic microsatellite loci were developed from S. marmoratus and were used to identify polymorphisms in 30 samples from a wild population. The allele locus number ranged from 2 to 7. Polymorphism data content ranged from 0.032 to 0.751. The observed and expected heterozygosity levels were 0.0333-0.9667 and 0.0328-0.7675, respectively. Two loci, Smd1-112 and Smd2-80, deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers will facilitate further studies of genetic diversity and genetic differentiation of S. marmoratus.
Fisheries College, Jimei University, Xiamen, China.
H W
HW
Deng
Fisheries College, Jimei University, Xiamen, China lizhongbao@jmu.edu.cn.
Z B
ZB
Li
Fisheries College, Jimei University, Xiamen, China.
G
G
Dai
Fisheries College, Jimei University, Xiamen, China.
Y
Y
Yuan
Fisheries College, Jimei University, Xiamen, China.
Y F
YF
Ning
Fisheries College, Jimei University, Xiamen, China.
J B
JB
Shangguan
Fisheries College, Jimei University, Xiamen, China.
Y S
YS
Huang
D000483:Alleles; D000818:Animals; D002681:China; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*