[Expression of transient receptor potential canonical 1 in ozone-induced inflammatory lung tissues in mice].
OBJECTIVE
To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of ozone-induced lung inflammation and explore its role in lung inflammation.


METHODS
In a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern blotting and immunohistochemistry.


RESULTS
Compared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice (P<0.01).


CONCLUSIONS
TRPC1 may play a role in ozone-induced lung inflammation in mice.
Department of Physiology, Guangzhou Medical University, Guangzhou 510182, China.E-mail: fuzhaodi2013@tom.com.
Zhaodi
Z
Fu
Lifen
L
Zhou
Jianrong
J
Huang
Shuyi
S
Guo
Jiechun
J
Zhang
Yongbiao
Y
Fang
Xiaoai
X
Liu
Qingzi
Q
Chne
Jianhua
J
Li
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D015870:Gene Expression; D007249:Inflammation / Q000473:pathology*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010126:Ozone / Q000009:adverse effects*; D011014:Pneumonia / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger; D050052:TRPC Cation Channels / Q000378:metabolism*
D012333:RNA, Messenger; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D010126:Ozone