Adsorption of hyaluronic acid on solid supports: role of pH and surface chemistry in thin film self-assembly.
Owing to its biocompatibility, resistance to biofouling, and desirable physicochemical and biological properties, hyaluronic acid (HA) has been widely used to modify the surface of various materials. The role of various physicochemical factors in HA adsorption remains, however, to be clarified. Herein, we employed quartz crystal microbalance with dissipation (QCM-D) in order to investigate HA adsorption at different pH conditions onto three substrates-silicon oxide, amine-terminated self-assembled monolayer (SAM) on gold, and carboxylic acid-terminated SAM on gold. The QCM-D experiments indicated specific pH conditions where either strong or weak HA adsorption occurs. The morphology of the adsorbed HA layers was investigated by atomic force microscopy (AFM), and we identified that strong HA adsorption produced a complete, homogenous and smooth HA layer, while weak HA adsorption resulted in rough and inhomogeneous HA layers. The observed specifics of the kinetics of HA adsorption, including a short initial linear phase and subsequent long non-linear phase, were described by using a mean-field kinetic model taking HA diffusion limitations and reconfiguration in the adsorbed state into account. The findings extend the physicochemical background of design strategies for improving the use of passive HA adsorption for surface modification applications.
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Jae-Hyeok
JH
Choi
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Seong-Oh
SO
Kim
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Eric
E
Linardy
Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, 500 Main Street, Cambridge, MA 02139, USA.
Erik C
EC
Dreaden
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore; Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia.
Vladimir P
VP
Zhdanov
Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, 500 Main Street, Cambridge, MA 02139, USA.
Paula T
PT
Hammond
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore; School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore. Electronic address: njcho@ntu.edu.sg.
Nam-Joon
NJ
Cho
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D017655:Silicon Compounds / Q000737:chemistry; D013499:Surface Properties
D000588:Amines; D002264:Carboxylic Acids; D010087:Oxides; D017655:Silicon Compounds; C116473:silicon monoxide; D006046:Gold; D006820:Hyaluronic Acid
Adsorption kinetics; Atomic force microscopy; Hyaluronic acid; Quartz crystal microbalance; Self-assembly; Thin film; pH condition