[Effect of tensile stress on human heel skin fibroblast proliferation in vitro].
OBJECTIVE
To observe the effect of tensile stress on human heel skin fibroblast proliferation in vitro, providing a theoretical basis for preventing the wound edge skin necrosis and nonunion after calcaneal fracture surgery.


METHODS
Fibroblast cells were taken from lateral heel skin of a 40 year-old-man, then cultured and subcultured in vitro. After that, they were divided into three groups: 0 hours group, 6 hours group and 24 hours group and were tested by tensile stress testing. The levels of TGF-β1 and IL-6 in nutrient fluid were measured. Transmission electron microscope and light microscope was applied for observe mitochondria and nucleus.


RESULTS
Under 10% of the tensile stress, mitochondria decreased, the levels of TGF-β1 and IL-6 in nutrient fluid were decreased and cell proliferation was inhibited gradually with time increasing.


CONCLUSIONS
The human lateral heel skin in a long-time tensile stress state is an important cause of wound edge skin necrosis and nonunion after calcaneus fracture surgery.
Jun-hua
JH
Li
Zhong-ming
ZM
Huang
Shao-hua
SH
Du
Li-gang
LG
Huang
D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry* / Q000166:cytology*; D006365:Heel / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D012867:Skin / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D013718:Tensile Strength; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D015850:Interleukin-6; D053773:Transforming Growth Factor beta1