Mechanisms on boron-induced alleviation of aluminum-toxicity in Citrus grandis seedlings at a transcriptional level revealed by cDNA-AFLP analysis.
The physiological and biochemical mechanisms on boron (B)-induced alleviation of aluminum (B)-toxicity in plants have been examined in some details, but our understanding of the molecular mechanisms underlying these processes is very limited. In this study, we first used the cDNA-AFLP to investigate the gene expression patterns in Citrus grandis roots responsive to B and Al interactions, and isolated 100 differentially expressed genes. Results showed that genes related to detoxification of reactive oxygen species (ROS) and aldehydes (i.e., glutathione S-transferase zeta class-like isoform X1, thioredoxin M-type 4, and 2-alkenal reductase (NADP+-dependent)-like), metabolism (i.e., carboxylesterases and lecithin-cholesterol acyltransferase-like 4-like, nicotianamine aminotransferase A-like isoform X3, thiosulfate sulfurtransferase 18-like isoform X1, and FNR, root isozyme 2), cell transport (i.e., non-specific lipid-transfer protein-like protein At2g13820-like and major facilitator superfamily protein), Ca signal and hormone (i.e., calcium-binding protein CML19-like and IAA-amino acid hydrolase ILR1-like 4-like), gene regulation (i.e., Gag-pol polyprotein) and cell wall modification (i.e., glycosyl hydrolase family 10 protein) might play a role in B-induced alleviation of Al-toxicity. Our results are useful not only for our understanding of molecular processes associated with B-induced alleviation of Al-toxicity, but also for obtaining key molecular genes to enhance Al-tolerance of plants in the future.
College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Xin-Xing
XX
Zhou
College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Lin-Tong
LT
Yang
Institute of Materia Medica, Fujian Academy of Medical Sciences, Fuzhou 350001, China.
Yi-Ping
YP
Qi
College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Peng
P
Guo
College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Key Laboratory for Plant Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Li-Song
LS
Chen
D000535:Aluminum / Q000378:metabolism / Q000633:toxicity*; D001895:Boron / Q000378:metabolism / Q000494:pharmacology*; D002957:Citrus / Q000187:drug effects* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018517:Plant Roots / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D018076:DNA, Complementary; D000535:Aluminum; D001895:Boron