Polymorphism identification in BMP15 and GDF9 genes and their association with egg production in chickens.
1. Mutations in growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are significantly associated with reproductive performance in mammals and the objective of the present study was to identify polymorphic sites and elucidate the association between genotypes in BMP15 and GDF9 and egg production. 2. Polymorphisms in BMP15 exon1 and GDF9 exon2 were detected by DNA sequencing and PCR-RFLP. Three SNPs were detected in each of BMP15 (A111G, C231T and C34T) and GDF9 (G593A, T824C and C896T). C34T leads to the substitution of Leu by Phe, which was predicted to affect protein function. 3. Results of the association analysis indicated that C34T had an effect on total egg production at 300 d of age (EN) and age at first laying (AFE). G593A affected EN and both C231T and C896T influenced AFE. The TGC1TGC1 diplotype in BMP15 had the highest EN. 4. In conclusion, EN may be significantly improved by marker-assisted selection of the BMP15 genotypes in maternal lines of Shaobo hens.
a Institute of Poultry Science , Chinese Academy of Agriculture Science , Yangzhou , P. R. China.
H Y
HY
Huang
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Z
Liang
S F
SF
Li
C M
CM
Li
Z H
ZH
Zhao
Q B
QB
Wang
D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics* / Q000378:metabolism; D001947:Breeding; D002645:Chickens / Q000235:genetics / Q000502:physiology*; D055429:Growth Differentiation Factor 9 / Q000235:genetics* / Q000378:metabolism; D010063:Ovum / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012098:Reproduction
D055430:Bone Morphogenetic Protein 15; D055429:Growth Differentiation Factor 9