BDNF-TrkB axis regulates migration of the lateral line primordium and modulates the maintenance of mechanoreceptor progenitors.
BDNF and its specialized receptor TrkB are expressed in the developing lateral line system of zebrafish, but their role in this organ is unknown. To tackle this problem in vivo, we used transgenic animals expressing fluorescent markers in different cell types of the lateral line and combined a BDNF gain-of-function approach by BDNF mRNA overexpression and by soaking embryos in a solution of BDNF, with a loss-of-function approach by injecting the antisence ntrk2b-morpholino and treating embryos with the specific Trk inhibitor K252a. Subsequent analysis demonstrated that the BDNF-TrkB axis regulates migration of the lateral line primordium. In particular, BDNF-TrkB influences the expression level of components of chemokine signaling including Cxcr4b, and the generation of progenitors of mechanoreceptors, at the level of expression of Atoh1a-Atp2b1a.
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Eugene V
EV
Gasanov
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Lola M
LM
Rafieva
Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Vladimir P
VP
Korzh
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D002465:Cell Movement; D053595:Embryonic Stem Cells / Q000502:physiology*; D029941:Fish Proteins / Q000502:physiology*; D015870:Gene Expression; D053403:Lateral Line System / Q000166:cytology / Q000196:embryology*; D008465:Mechanoreceptors / Q000502:physiology*; D020813:Receptor, trkB / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*
D019208:Brain-Derived Neurotrophic Factor; D029941:Fish Proteins; D020813:Receptor, trkB