[Expression of limulus Factor C in silkworm larvae by Bac-to-Bac/BmNPV baculovirus expression system].
Limulus Factor C, a serine protease zymogen from the amoebocytes of the limulus, has high affinity for endotoxin. When Factor C is activated by endotoxin, it hydrolyses artificial tripeptide substrate and measurable products are released, so it can be used as an alternative reagent for endotoxin analysis. Factor C gene of Tachypleus tridentatus was obtained through RT-PCR and the recombinant protein was expressed by Bac-to-Bac/BmNPV baculovirus expression system in silkworm larvae. The activity of Factor C was detected with diluted serum of silkworm larvae, and the sensitivity of endotoxin detected was 0.2 EU/mL when the serum was diluted at 1:500. The silkworm larvae expressed limulus Factor C could be used to develop a new low-cost endotoxin test reagent.
Jing
J
Qi
Tao
T
Liu
Zhen
Z
Li
Chengliang
C
Gong
Haiping
H
Wu
Chun
C
Zhang
D000818:Animals; D060829:Arthropod Proteins / Q000096:biosynthesis*; D016367:Baculoviridae; D012831:Bombyx / Q000378:metabolism*; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005822:Genetic Vectors; D007814:Larva / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D012697:Serine Endopeptidases / Q000096:biosynthesis*
D060829:Arthropod Proteins; D004792:Enzyme Precursors; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; C057151:Limulus clotting factor C