Curcumin restores mitochondrial functions and decreases lipid peroxidation in liver and kidneys of diabetic db/db mice.
BACKGROUND
Nitrosative and oxidative stress play a key role in obesity and diabetes-related mitochondrial dysfunction. The objective was to investigate the effect of curcumin treatment on state 3 and 4 oxygen consumption, nitric oxide (NO) synthesis, ATPase activity and lipid oxidation in mitochondria isolated from liver and kidneys of diabetic db/db mice.


RESULTS
Hyperglycaemia increased oxygen consumption and decreased NO synthesis in liver mitochondria isolated from diabetic mice relative to the control mice. In kidney mitochondria, hyperglycaemia increased state 3 oxygen consumption and thiobarbituric acid-reactive substances (TBARS) levels in diabetic mice relative to control mice. Interestingly, treating db/db mice with curcumin improved or restored these parameters to normal levels; also curcumin increased liver mitochondrial ATPase activity in db/db mice relative to untreated db/db mice.


CONCLUSIONS
These findings suggest that hyperglycaemia modifies oxygen consumption rate, NO synthesis and increases TBARS levels in mitochondria from the liver and kidneys of diabetic mice, whereas curcumin may have a protective role against these alterations.
Departamento de Ciencias Médicas, Universidad de Guanajuato, C.P. León, 37320, Obregón, GTO, México. sugo9883@hotmail.com.
María G
MG
Soto-Urquieta
Departamento de Ciencias Médicas, Universidad de Guanajuato, C.P. León, 37320, Obregón, GTO, México. lobris@yahoo.com.
Sergio
S
López-Briones
Departamento de Ciencias Médicas, Universidad de Guanajuato, C.P. León, 37320, Obregón, GTO, México. vicpe@yahoo.com.
Victoriano
V
Pérez-Vázquez
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, MICH, México. fredisaav2002@yahoo.com.mx.|University of Texas Medical Branch at Galveston, Galveston, TX, USA. fredisaav2002@yahoo.com.mx.
Alfredo
A
Saavedra-Molina
Departamento de Biología, Universidad de Guanajuato, León, GTO, México. gonzang@ugto.mx.
Gloria A
GA
González-Hernández
Departamento de Ciencias Médicas, Universidad de Guanajuato, C.P. León, 37320, Obregón, GTO, México. joelre@ugto.mx.
Joel
J
Ramírez-Emiliano
D000251:Adenosine Triphosphatases / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D019069:Cell Respiration / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000178:diet therapy* / Q000503:physiopathology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005838:Genotype; D006943:Hyperglycemia / Q000178:diet therapy / Q000209:etiology; D007668:Kidney / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D000068618:Selective Breeding
D009569:Nitric Oxide; D000251:Adenosine Triphosphatases; D003474:Curcumin