Acoustic-frequency vibratory stimulation regulates the balance between osteogenesis and adipogenesis of human bone marrow-derived mesenchymal stem cells.
Osteoporosis can be associated with the disordered balance between osteogenesis and adipogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs). Although low-frequency mechanical vibration has been demonstrated to promote osteogenesis, little is known about the influence of acoustic-frequency vibratory stimulation (AFVS). BM-MSCs were subjected to AFVS at frequencies of 0, 30, 400, and 800 Hz and induced toward osteogenic or adipogenic-specific lineage. Extracellular matrix mineralization was determined by Alizarin Red S staining and lipid accumulation was assessed by Oil Red O staining. Transcript levels of osteogenic and adipogenic marker genes were evaluated by real-time reverse transcription-polymerase chain reaction. Cell proliferation of BM-MSCs was promoted following exposure to AFVS at 800 Hz. Vibration at 800 Hz induced the highest level of calcium deposition and significantly increased mRNA expression of COL1A1, ALP, RUNX2, and SPP1. The 800 Hz group downregulated lipid accumulation and levels of adipogenic genes, including FABP4, CEBPA, PPARG, and LEP, while vibration at 30 Hz supported adipogenesis. BM-MSCs showed a frequency-dependent response to acoustic vibration. AFVS at 800 Hz was the most favorable for osteogenic differentiation and simultaneously suppressed adipogenesis. Thus, acoustic vibration could potentially become a novel means to prevent and treat osteoporosis.
Orthopaedic Institute, Soochow University, Department of Orthopaedics, the 1st Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, China ; School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu 215123, China.
Xi
X
Chen
Orthopaedic Institute, Soochow University, Department of Orthopaedics, the 1st Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, China.
Fan
0000-0001-5357-1374
F
He
Orthopaedic Institute, Soochow University, Department of Orthopaedics, the 1st Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, China.
Dong-Yan
DY
Zhong
Orthopaedic Institute, Soochow University, Department of Orthopaedics, the 1st Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, China.
Zong-Ping
ZP
Luo
D050156:Adipogenesis; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D013016:Sound; D014732:Vibration
D000943:Antigens, Differentiation