Circulating mesenchymal stem cells in patients with hypertrophic cardiomyopathy.
BACKGROUND
This study examines the mobilization of mesenchymal stem cells (MSCs) in patients with hypertrophic cardiomyopathy (HCM) compared to healthy individuals. The pathogenesis of myocardial hypertrophy in HCM is not fully understood. MSCs are involved in the process of neovascularization, fibrosis, and ventricular wall remodeling.


RESULTS
We included 40 patients with HCM and 23 healthy individuals. Using flow cytometry, we measured MSCs in peripheral blood, as a population of CD45-/CD34-/CD90+ cells and also as a population of CD45-/CD34-/CD105+ cells. The resulting MSC counts were expressed as percentages of the total cells. Patients with HCM were found to have a greater percentage of circulating CD45-/CD34-CD34-/CD90+ cells compared to controls (0.0041±0.005% vs. 0.0007±0.001%, respectively, P<.001). No significant difference in circulating CD45-/CD34-/CD105+ cells in the peripheral blood was found between HCM patients and controls (0.016±0.018% vs. 0.012±0.014%, respectively, P=.4). Notably, circulating CD45-/CD34-/CD90+ cells were positively correlated with left ventricular mass index (r=0.54, P<.001).


CONCLUSIONS
Patients with HCM reveal an increased mobilization of MSCs compared to healthy individuals. Although further research is needed to reveal the clinical significance of our findings, our data open a new dimension in the pathophysiology of the disease and may indicate new future therapeutic possibilities.
Department of Cardiology, Heraklion University Hospital, Crete, Greece. Electronic address: maryemarke@yahoo.gr.
Maria E
ME
Marketou
Department of Cardiology, Heraklion University Hospital, Crete, Greece.
Fragiskos I
FI
Parthenakis
Molecular Cardiology Laboratory, School of Medicine, University of Crete, Greece.
Athanasia
A
Kalyva
Department of Hematology, Heraklion University Hospital, Crete, Greece.
Charalampos
C
Pontikoglou
Department of Cardiology, Heraklion University Hospital, Crete, Greece.
Spyros
S
Maragkoudakis
Molecular Cardiology Laboratory, School of Medicine, University of Crete, Greece.
Joanna E
JE
Kontaraki
Department of Cardiology, Heraklion University Hospital, Crete, Greece.
Evangelos A
EA
Zacharis
Department of Cardiology, Heraklion University Hospital, Crete, Greece.
Alexandros
A
Patrianakos
Division of Biostatistics, School of Medicine, University of Crete, Greece.
Gregory
G
Chlouverakis
Department of Hematology, Heraklion University Hospital, Crete, Greece.
Helen A
HA
Papadaki
Department of Cardiology, Heraklion University Hospital, Crete, Greece.
Panos E
PE
Vardas
D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D002312:Cardiomyopathy, Hypertrophic / Q000097:blood* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D008875:Middle Aged
D015703:Antigens, CD
Flow cytometry; Hypertrophic cardiomyopathy; Mesenchymal stem cells