Submerged Culture of Grifola gargal and G. sordulenta (Higher Basidiomycetes) from Argentina as a Source of Mycelia with Antioxidant Activity.
Submerged culture is an alternative mycelium source for Grifola gargal and G. sordulenta, two rare edible mushrooms related to Grifola frondosa. This work studies their mycelia as a source of antioxidants. The efficient concentrations of methanolic extracts in both radical scavenging (RS) and reducing power (RP) abilities in G. gargal and in G. sordulenta showed a high antioxidant activity. In the experimental design used, the antioxidant activity mainly depended on the culture conditions rather than on the media composition. Irrespective of the basal culture medium, mycelium methanolic extracts of G. sordulenta obtained from culture in Erlenmeyer flasks showed equivalents to ascorbic acid (EQ(AA)) RS-EQ(AA) and RP-EQ(AA) contents higher than the corresponding values obtained with jar cultures. Under stationary cultivation, G. sordulenta produced approximately 50% higher content in both RS-EQ(AA) and RP-EQ(AA) than the medicinal mushroom G. frondosa. Phenolics correlated with RS-EQ(AA) and RP-EQ(AA) in G. gargal and with RP-EQ(AA) in G. sordulenta; besides, thin-layer chromatography showed these compounds to be at least in part related to the RS capacity. It is concluded that G. gargal and G. sordulenta mycelia are excellent sources of antioxidant metabolites.
Laboratory of Biotechnology of Edible and Medicinal Mushrooms, Centro de Recursos Naturales Renovables de la Zona Semiarida (CERZOS), Consejo Nacional de Investigaciones Cientificas y Tecnicas - Universidad Nacional del Sur (CONICET-UNS), Bahia Blanca.
Pablo Daniel
PD
Postemsky
Departamento de Agronomia, Universidad Nacional del Sur, Bahia Blanca, Argentina; Laboratorio de Biotecnologia de Hongos Comestibles y Medicinales (LBHCM), CERZOS-CONICET-UNS Argentina.
Néstor Raúl
NR
Curvetto
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001118:Argentina; D001205:Ascorbic Acid / Q000737:chemistry; D018929:Cell Culture Techniques; D048691:Grifola / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D000975:Antioxidants; D010936:Plant Extracts; D001205:Ascorbic Acid