Twin-column CaptureSMB: a novel cyclic process for protein A affinity chromatography.
A twin-column counter-current chromatography processes, CaptureSMB, was used for the protein A affinity capture of a monoclonal antibody (mAb). By means of sequential loading, the process improves the utilization of the stationary phase by achieving loadings much closer to the static binding capacity of the resin in comparison to batch chromatography. Using a mAb capture case study with protein A affinity chromatography, the performance and product quality obtained from CaptureSMB and batch processes were compared. The effect of the flow rate, column length and titer concentration on the process performance and product quality were evaluated. CaptureSMB showed superior performance compared to batch chromatography with respect to productivity, capacity utilization, product concentration and buffer consumption. A simplified economic evaluation showed that CaptureSMB could decrease resin costs of 10-30% depending on the manufacturing scenario.
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Switzerland(1).
Monica
M
Angarita
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Switzerland(1); ChromaCon AG, Zürich, Switzerland(2).
Thomas
T
Müller-Späth
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Switzerland(1).
Daniel
D
Baur
JSR Life Sciences, JSR Micro N.V., Leuven, Belgium.
Roel
R
Lievrouw
JSR Life Sciences, JSR Micro N.V., Leuven, Belgium.
Geert
G
Lissens
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Switzerland(1). Electronic address: massimo.morbidelli@chem.ethz.ch.
Massimo
M
Morbidelli
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002021:Buffers; D002846:Chromatography, Affinity / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D013205:Staphylococcal Protein A / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D002021:Buffers; D013205:Staphylococcal Protein A
Breakthrough curve; Monoclonal antibody; Periodic counter-current chromatography; Protein A affinity chromatography; Sequential capture