Metabolomics specificity of tuberculosis plasma revealed by (1)H NMR spectroscopy.
Tuberculosis (TB) is a communicable disease of major global importance and causes metabolic disorder of the patients. In a previous study, we found that the plasma metabolite profile of TB patients differs from that of healthy control subjects based on nuclear magnetic resonance (NMR) spectroscopy. In order to evaluate the TB specificity of the metabolite profile, a total of 110 patients, including 40 with diabetes, 40 with malignancy, and 30 with community-acquired pneumonia (CAP), assessed by NMR spectroscopy, and compared to those of patients with TB. Based on the orthogonal partial least-squares discriminant analysis (OPLS-DA), the metabolic profiles of these diseases were significant different, as compared to the healthy controls and TB patients, respectively. The score plots of the OPLS-DA model demonstrated that TB was easily distinguishable from diabetes, CAP and malignancy. Plasma levels of ketone bodies, lactate, and pyruvate were increased in TB patient compared to healthy control, but lower than CAP and malignancy. We conclude that the metabolic profiles were TB-specific and reflected MTB infection. Our results strongly support the NMR spectroscopy-based metabolomics could contribute to an improved understanding of disease mechanisms and may offer clues to new TB clinic diagnosis and therapies.
Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China; Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Tibet University for Nationalities School of Medicine, Xianyang, Shanxi 712082, China. Electronic address: abby_aiping@126.com.
Aiping
A
Zhou
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: nijinjing45@163.com.
Jinjing
J
Ni
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: xvzhihong@126.com.
Zhihong
Z
Xu
Shanghai Institute of Immunology, Shanghai 200025, China. Electronic address: ywang@sibs.ac.cn.
Ying
Y
Wang
Renji Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China. Electronic address: zhanghaomin1976@163.com.
Haomin
H
Zhang
Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China. Electronic address: wwj1210@126.com.
Wenjuan
W
Wu
Shanghai Public Health Clinical Center, Shanghai 201508, China. Electronic address: tubercle@shaphc.org.
Shuihua
S
Lu
Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address: petros@jhmi.edu.
Petros C
PC
Karakousis
Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China; Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: yfyao@sjtu.edu.cn.
Yu-Feng
YF
Yao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D017714:Community-Acquired Infections / Q000097:blood / Q000175:diagnosis; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007657:Ketone Bodies / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis; D011014:Pneumonia / Q000097:blood / Q000175:diagnosis; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D066244:Proton Magnetic Resonance Spectroscopy; D019289:Pyruvic Acid / Q000097:blood; D014376:Tuberculosis / Q000097:blood* / Q000175:diagnosis / Q000382:microbiology
D015415:Biomarkers; D007657:Ketone Bodies; D019344:Lactic Acid; D019289:Pyruvic Acid
Community-acquired pneumonia; Diabetes; Malignancy; Metabolite; Plasma; Tuberculosis