[Optimization of submerged culture conditions for Xylaria striata mycelium].
OBJECTIVE
To establish the optimum submerged culture condition of Xylaria striata mycelium.


METHODS
One-factor-at-a-time method and orthogonal experiment design were applied and the dry weight of mycelium was tested for evaluation the biomass of the growth of Xylaria striata mycelium.


RESULTS
The results from one-factor-at-a-time experiments showed that maltose, glucose or corn powder could be used as the best carbon source and the optimum nitrogen source was soybean powder. The best ratio of carbon source to nitrogen source was 5:1. In addition, the higher dry weight of mycelium was found at pH 6 when 2 mycelial discs were inoculated and fermented for 7 days. Otherwise, the growth of mycelium was observed to be promoted significantly by addition with K,Mg,P and VB1. The consequence of orthogonal experiment showed that the optimum carbon source and nitrogen source were maltose 4% and soybean powder 0.8%, respectively,and the highest mycelium biomass could be obtained at pH 7 and 25 °C shaking for 13 days.


CONCLUSIONS
A large amount of mycelium will be obtained under the optimum condition of liquid culture for Xylaria striata.
Xia
X
Liu
Cong
C
Gao
Yu-Ting
YT
Zhong
Xiao-Hong
XH
Yuan
Xin-Sheng
XS
He
Lin
L
Ma
D018533:Biomass; D019149:Bioreactors; D002244:Carbon; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development*; D009584:Nitrogen
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen