Nanopore-based DNA-probe sequence-evolution method unveiling characteristics of protein-DNA binding phenomena in a nanoscale confined space.
Almost all of the important functions of DNA are realized by proteins which interact with specific DNA, which actually happens in a limited space. However, most of the studies about the protein-DNA binding are in an unconfined space. Here, we propose a new method, nanopore-based DNA-probe sequence-evolution (NDPSE), which includes up to 6 different DNA-probe systems successively designed in a nanoscale confined space which unveil the more realistic characteristics of protein-DNA binding phenomena. There are several features; for example, first, the edge-hindrance and core-hindrance contribute differently for the binding events, and second, there is an equilibrium between protein-DNA binding and DNA-DNA hybridization.
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Nannan
N
Liu
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Zekun
Z
Yang
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Xiaoding
X
Lou
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Benmei
B
Wei
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Juntao
J
Zhang
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Pengcheng
P
Gao
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Ruizuo
R
Hou
§Key Laboratory for Large-Format Battery Materials and Systems, Ministry of Education School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.|‡National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430074, P. R. China.
Fan
F
Xia
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D058608:Nanopores; D011506:Proteins / Q000737:chemistry*
D015342:DNA Probes; D011506:Proteins; D004247:DNA