Lack of influence of the ACE1 gene I/D polymorphism on the formation and growth of benign uterine leiomyoma in Turkish patients.
Uterine leiomyomas (ULM), are benign tumors of the smooth muscle cells of the myometrium. They represent a common health problem and are estimated to be present in 30-70% of clinically reproductive women. Abnormal angiogenesis and vascular-related growth factors have been suggested to be associated with ULM growth. The angiotensin-I converting enzyme (ACE) is related with several tumors. The aim of this study was to identify possible correlation between ULM and the ACE I/D polymorphism, to evaluate whether the ACE I/D polymorphism could be a marker for early diagnosis and prognosis. ACE I/D was amplified with specific primer sets recognizing genomic DNA from ULM (n=72) and control (n=83) volunteers and amplicons were separated on agarose gels. The observed genotype frequencies were in agreement with Hardy-Weinberg equilibrium (χ2=2.162, p=0.339). There was no association between allele frequencies and study groups (χ2=0.623; p=0.430 for ACE I allele, χ2=0.995; p=0.339 for ACE D allele). In addition, there were no significant differences between ACE I/D polymorphism genotype frequencies and ULM range in size and number (χ2=1.760; p=0.415 for fibroid size, χ2=0.342; p=0.843 for fibroid number). We conclude that the ACE gene I/D polymorphism is not related with the size or number of ULM fibroids in Turkish women. Thus it cannot be regarded as an early diagnostic parameter nor as a risk estimate for ULM predisposition.
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul Turkey E-mail : turgay.isbir@yeditepe.edu.tr, tisbir@superonline.com.
Guldal Inal
GI
Gultekin
Seda Gulec
SG
Yilmaz
Ozlem Timirci
OT
Kahraman
Hande
H
Atasoy
A Burak
AB
Dalan
Rukset
R
Attar
Ahmet
A
Buyukoren
Nazli
N
Ucunoglu
Turgay
T
Isbir
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D064368:Healthy Volunteers; D006801:Humans; D007889:Leiomyoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D008297:Male; D009367:Neoplasm Staging; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors; D014421:Turkey
D007703:Peptidyl-Dipeptidase A