Effect of the tyrosinase inhibitor (S)-N-trans-feruloyloctopamine from garlic skin on tyrosinase gene expression and melanine accumulation in melanoma cells.
In our searching for novel tyrosinase inhibitors from natural sources, (S)-N-trans-feruloyloctopamine isolated from garlic skin was found to be a potential mushroom tyrosinase inhibitor. Here, we examined the effects of the potential tyrosinase inhibitor in B16F10 cells on intracellular melanin contents, cytotoxicity, and the signaling mechanism involved in the expression of tyrosinase. The results showed the inhibitor displayed little or no cytotoxicity at all concentrations examined and decreased the relative melanin contents in a dose-dependent manner in the α-MSH-stimulated B16F10 cells. Real-time PCR and Western blot analysis showed that it inhibits melanogenesis signaling by down-regulates mRNA and protein expression levels of tyrosinase, which leads to a lower melanin contents. These results suggested that (S)-N-trans-feruloyloctopamine was an ideal tyrosinase inhibitor, and could be used in food and medical industry.
Gansu College of Traditional Chinese Medicine, 35 Dingxi East Road, Lanzhou 730000, People's Republic of China.
Yan
Y
Wu
School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China.
Zheng-Rong
ZR
Wu
School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China.
Peng
P
Chen
School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China.
Yang-Li
Gansu College of Traditional Chinese Medicine, 35 Dingxi East Road, Lanzhou 730000, People's Republic of China.
Wan-Rong
WR
Deng
School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China.
You-Quan
YQ
Wang
School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of China. Electronic address: wuzhrong@163.com.
Hong-Yu
HY
Li
D000363:Agaricales / Q000201:enzymology; D000818:Animals; D045744:Cell Line, Tumor; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008543:Melanins / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D009655:Octopamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship
D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D008543:Melanins; C498173:N-feruloyloctopamine; D009655:Octopamine; D014442:Monophenol Monooxygenase
(S)-N-trans-Feruloyloctopamine; Anti-melanogenic effect; Cytotoxicity; Tyrosinase expression