Immediate human pulp response to ethanol-wet bonding technique.
OBJECTIVE
To evaluate the short-term response of human pulps to ethanol-wet bonding technique.


METHODS
Deep class V cavities were prepared on 17 sound premolars and divided into three groups. After acid-etching, the cavities from groups 1 (G1) and 2 (G2) were filled with 100% ethanol or distilled water, respectively, for 60 s before the application of Single Bond 2. In group 3 (G3, control), the cavity floor was lined with calcium hydroxide before etching and bonding. All cavities were restored with resin composite. Two teeth were used as intact control. The teeth were extracted 48h after the clinical procedures. From each tooth serial sections were obtained and stained with haematoxylin and eosin (H/E) and Masson's trichrome. Bacteria microleakage was assessed using Brown & Brenn. All sections were blindly evaluated for five histological features.


RESULTS
Mean remaining dentine thickness was 463±65μm (G1); 425±184μm (G2); and 348±194μm (G3). Similar pulp reactions followed ethanol- or water-wet bonding techniques. Slight inflammatory responses and disruption of the odontoblast layer related to the cavity floor were seen in all groups. Stained bacteria were not detected in any cavities. Normal pulp tissue was observed in G3 except for one case.


CONCLUSIONS
After 48h, ethanol-wet bonding does not increase pulpal damage compared to water-wet bonding technique.


CONCLUSIONS
Ethanol-wet bonding may increase resin-dentine bond durability. This study reported the in vivo response of human pulp tissue when 100% ethanol was applied previously to an etch-and-rinse simplified adhesive system.
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
Débora Lopes Salles
DL
Scheffel
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Av. Décima Primeira Avenida, 62000 - Setor Leste Universitário, Goiânia, Goiás 74605-020, Brazil.
Nancy Tomoko
NT
Sacono
Department of Dentistry, Dental School, Federal University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal 70910-900, Brazil.
Ana Paula Dias
AP
Ribeiro
Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
Diana Gabriela
DG
Soares
Department of Physiology and Pathology, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
Fernanda Gonçalves
FG
Basso
Department of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, CL-2112, Augusta, GA 30912-1129, USA.
David
D
Pashley
Department of Physiology and Pathology, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil.
Carlos Alberto de Souza
CA
Costa
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil. Electronic address: jhebling@foar.unesp.br.
Josimeri
J
Hebling
D000293:Adolescent; D001641:Bicuspid; D001672:Biocompatible Materials / Q000627:therapeutic use; D001840:Dental Bonding / Q000379:methods*; D003736:Dental Cavity Lining; D003737:Dental Cavity Preparation; D003763:Dental Leakage / Q000382:microbiology; D003782:Dental Pulp / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D003789:Dental Pulp Exposure / Q000628:therapy; D003804:Dentin / Q000382:microbiology / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009804:Odontoblasts / Q000473:pathology
D001672:Biocompatible Materials; D017220:Dentin-Bonding Agents; D000431:Ethanol
Biocompatibility; Dental pulp; Dentine bonding agent; Ethanol