Virus-induced gene silencing (VIGS) for functional genomics in rice using Rice tungro bacilliform virus (RTBV) as a vector.
The large-scale functional analysis of genes in plants depends heavily on robust techniques for gene silencing. Virus-induced gene silencing (VIGS) is a transient gene silencing method for plants, triggered by the inoculation of a modified viral vector carrying a fragment of the gene targeted for silencing. Here we describe a VIGS protocol for rice, based on the Rice tungro bacilliform virus (RTBV, a DNA virus). We present an updated and detailed protocol for silencing of the gene encoding Phytoene desaturase in rice, using the RTBV-VIGS system.
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
Ravi
R
Kant
Shweta
S
Sharma
Indranil
I
Dasgupta
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000502:physiology / Q000821:virology; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques / Q000379:methods*; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D052641:Tungrovirus / Q000235:genetics*
D010940:Plant Proteins; D010088:Oxidoreductases; C022398:phytoene dehydrogenase