Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography.
High-speed counter-current chromatography (HSCCC) was successfully applied for the preparative separation and purification of N-feruloyl serotonin (NF) and N-(p-coumaroyl) serotonin (NP) from safflower seed meal. After the measurement of partition coefficient of the two target compounds in the two-phase solvent systems, the HSCCC was performed well with a two-phase solvent system composed of CHCl3-methanol-0.1 M HCl at a volume ratio of 1 : 1 : 1, v/v. The upper phase was used as stationary phase and the lower phase was used as mobile phase. Under the optimized condition, 7.5 mg NF and 6.9 mg NP were separated from 40 mg crude sample with the purity of 98.8 and 97.3%, respectively. The structures of the isolated compounds were identified by (1)H NMR and (13)C NMR.
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China University of the Chinese Academy of Sciences, Beijing 100049, China.
Qiulong
Q
Zhang
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China University of the Chinese Academy of Sciences, Beijing 100049, China.
Na
N
Hu
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China University of the Chinese Academy of Sciences, Beijing 100049, China.
Wencong
W
Li
Qinghai Red Cross Hospital, Xining 810099, China.
Chenxi
C
Ding
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China University of the Chinese Academy of Sciences, Beijing 100049, China.
Tao
T
Ma
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China.
Bo
B
Bai
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China.
Honglun
H
Wang
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China.
Yourui
Y
Suo
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China.
Xiaoyan
X
Wang
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xin'ning Road, Xining, Qinghai 810008, PR China Qinghai Nationalities University, Xining 810007, China Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province, Xining 810001, China cxding@nwipb.cas.cn.
Chenxu
C
Ding
D031187:Carthamus tinctorius / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012639:Seeds / Q000737:chemistry*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
C512561:N-feruloylserotonin; D012701:Serotonin; C114774:N-(p-coumaroyl)serotonin