Mining the granule proteome: a potential source of endocrine biomarkers.
Proteomics of secretory granules is an emerging strategy for identifying secreted proteins, including potentially novel candidate biomarkers and peptide hormones. In addition, proteomics can provide information about the abundance, localization and structure (post-translational modification) of granule proteins and peptides. Analytical strategies within this research line include so-called 'subtractive proteomics', 'peptidomics' and granule purification by the use of multiple gradient centrifugations. Here we review the literature, and describe the challenges and opportunities in proteomics of secretory granules.
Department of Clinical Biochemistry (KB3014), Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
Jakob
J
Albrethsen
Jens P
JP
Goetze
Anders H
AH
Johnsen
D015415:Biomarkers / Q000378:metabolism; D055098:Endocrine Cells / Q000166:cytology*; D006801:Humans; D010455:Peptides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D022142:Secretory Vesicles / Q000378:metabolism*
D015415:Biomarkers; D010455:Peptides; D020543:Proteome
gradient centrifugation; granule; peptide hormone; peptidomics; proteomics; secretomics; secretory vesicle