Repeated exposure of male mice to low doses of lipopolysaccharide: dose and time dependent development of behavioral sensitization and tolerance in an automated light-dark anxiety test.
Although lipopolysaccharide (LPS) is widely used to examine immune behavior relationships there has been little consideration of the effects of low doses and the roles of sensitization and, or tolerance. Here low doses of LPS (1.0, 5.0 and 25.0 μg/kg) were peripherally administered to male mice on Days 1, 4, 28 and 32 after a baseline recording of anxiety-like behaviors in an automated light-dark apparatus (total time in the light chamber, number of light-dark transitions, nose pokes into the light chamber). LPS at 1.0 μg/kg, while having no significant effects on anxiety-like behaviors in the light-dark test on Days 1 and 4, displayed sensitization with the mice exhibiting significantly enhanced anxiety-like responses on Days 28 and 32. LPS at 5.0 μg/kg had no consistent significant effects on anxiety-like behavior on Days 1 and 4, with sensitization and enhanced anxiety-like behaviors on Day 28 followed by tolerance on Day 32. LPS at 25 μg/kg significantly enhanced anxiety-like behaviors on Day 1, followed by tolerance on Day 4, which was not evident by Day 28 and re-emerged on Day 32. There was a similar overall pattern of sensitization and tolerance for LPS-induced decreases in locomotor activity in the safe dark chamber, without, however, any significant effects on activity in the riskier light chamber. This shows that low doses of LPS induce anxiety-like behavior and these effects are subject to sensitization and tolerance in a dose, context, and time related manner.
Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA, USA.
Tomek J
TJ
Banasikowski
Department of Psychology, University of Western Ontario, London, ON, Canada.
Caylen J
CJ
Cloutier
Department of Psychology, University of Western Ontario, London, ON, Canada; Neuroscience Graduate Program, University of Western Ontario, London, ON, Canada.
Klaus-Peter
KP
Ossenkopp
Department of Psychology, University of Western Ontario, London, ON, Canada; Neuroscience Graduate Program, University of Western Ontario, London, ON, Canada. Electronic address: kavalier@uwo.ca.
Martin
M
Kavaliers
D000818:Animals; D001007:Anxiety / Q000276:immunology*; D001424:Bacterial Infections / Q000503:physiopathology* / Q000523:psychology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000633:toxicity*; D008297:Male; D051379:Mice; D009043:Motor Activity; D009483:Neuropsychological Tests; D010775:Photic Stimulation; D011897:Random Allocation; D013997:Time Factors
D008070:Lipopolysaccharides
Bacterial infection; Endotoxin priming; Immune response; LPS; Locomotor activity; Sickness behavior