Specific in vitro interferon-gamma and IL-2 production as biomarkers during treatment of chronic Q fever.
BACKGROUND
Antibiotic treatment of chronic Q fever is cumbersome and of long duration. To monitor treatment, there is a need for alternative biomarkers. Coxiella burnetii-specific interferon (IFN)-γ and interleukin (IL)-2 production reflect the type of effector and memory T-cell response. In chronic Q fever, C. burnetii-specific IFN-γ production is higher and IL-2 production is lower than in individuals with past Q fever. Here we explore whether C. burnetii-specific IFN-γ and IL-2 production correlate to treatment response.


METHODS
We studied the longitudinal C. burnetii-specific IFN-γ/IL-2 ratio in fifteen proven chronic Q fever patients. All patients were followed for at least 18 months during antibiotic treatment. Treatment was considered successful when clinical recovery was observed, a positive PCR for C. burnetii DNA in blood became persistently negative, anti-phase I IgG showed a fourfold decrease or more, and imaging techniques showed disappearance of infectious foci.


RESULTS
Overall, the IFN-γ/IL-2 ratio declined when patients experienced a successful treatment outcome. When treatment failed, IFN-γ/IL-2 ratios did not significantly decrease. The median (±IQR) slope of the longitudinal IFN-γ/IL-2 ratio with successful treatment was -2.10 (-7.02 to -0.06), and -0.15 (-1.13 to 0.25) with unsuccessful treatment (P = 0.19). Q fever endocarditis patients had higher IFN-γ/IL-2 ratios than patients with endovascular infections.


CONCLUSIONS
We propose that the IFN-γ/IL-2 ratio can be used as an additional biomarker for monitoring chronic Q fever treatment, with declining ratios being indicative of successful treatment.
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Teske
T
Schoffelen
Department of Medical Microbiology, Laboratory for Pathology and Medical Microbiology Veldhoven, Netherlands.
Marjolijn C
MC
Wegdam-Blans
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Anne
A
Ammerdorffer
Department of Internal Medicine, Catharina Hospital Eindhoven, Netherlands.
Marjolijn J H
MJ
Pronk
Department of Internal Medicine, Elkerliek Hospital Helmond, Netherlands.
Yvonne E P
YE
Soethoudt
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Mihai G
MG
Netea
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Jos W M
JW
van der Meer
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Chantal P
CP
Bleeker-Rovers
Department of Internal Medicine, Radboud University Medical Center Nijmegen, Netherlands.
Marcel
M
van Deuren
Coxiella burnetii; Q fever; biomarker; cell-mediated immunity; interferon-gamma; interleukin-2; serology; treatment