Special Issue on "Analytical Methods for Oxidized Biomolecules and Antioxidants" The use of isoprostanoids as biomarkers of oxidative damage, and their role in human dietary intervention studies.
Isoprostanoids are a group of non-enzymatic oxidized lipids from polyunsaturated fatty acids. They are commonly used as biomarkers for oxidative damage, to assess in vivo lipid peroxidation in diseases related to the vascular system and neurodegeneration. Currently, there is a mismatch with the outcome in the use of these biomarkers in intervention studies, particularly when testing the effect of antioxidants such as vitamins C and E, or zinc, or a cocktail of these, with other food components. Much of this is because the biomarkers, the method of measurement, and the duration of supplementation are unsuitable. In this review, we will highlight the formation of isoprostanoids from their respective fatty acids, and their application as biomarkers for oxidative damage in vivo, considering human dietary intervention studies evaluating plasma and urine, using mass spectrometry techniques.
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Universités of Montpellier , France.
J-M
JM
Galano
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Lee
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Durand
J C-Y
JC
Lee
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D004032:Diet / Q000009:adverse effects; D019587:Dietary Supplements; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D028421:Isoprostanes / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015227:Lipid Peroxidation / Q000187:drug effects; D013058:Mass Spectrometry / Q000379:methods; D015596:Nutrition Assessment; D009752:Nutritional Status; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011237:Predictive Value of Tests; D053719:Tandem Mass Spectrometry; D016896:Treatment Outcome
D000975:Antioxidants; D015415:Biomarkers; D028421:Isoprostanes
antioxidants; dihomo-isoprostanes; isoprostanes; mass spectrometry; neuroprostanes; phytoprostanes