Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy.
We investigated the expression of membrane complement regulators (CRegs), CD46, CD55 and CD59 in human mesothelial cells, and correlated with clinical background and level of complement (C) activation products in peritoneal dialysis (PD) fluids (PDF) to clarify influence of the C activation system in PD patients. Expression of CRegs was assessed on primary cultures of mesothelial cells (HPMC) harvested from PD fluid of 31 PD patients. Because expression of CD55 but not CD46 and CD59 in mesothelial cells was significantly correlated to value of dialysate-to-plasma creatinine concentration ratio (D/P Cre) (p<0.005) as an indicator of peritoneal function, we focused on analysis of CD55 expression of HPMCs in comparison with levels of C activation products in the PDF of the PD patients, and their background factors. When comparing expression of the CRegs between systemic neutrophils and HPMC, no correlation was observed, supporting that change of CRegs' expression in HPMC was independently occurring in the peritoneum. Expression of CD55 protein in HPMC was closely correlated with expression at the mRNA level (p<0.0001) and was inversely correlated with levels of sC5b-9 (p<0.05), but not C3, C4, IL6 and CA125 in the PDF. Complications of diabetes, usage of icodextrin and residual renal function were not correlated with change of CD55 expression in HPMCs. Our data show that the process of PD therapy modifies expression of CD55 on peritoneal mesothelium and triggers local C activation. These findings support efforts to modify PD therapy to limit effects on activation and regulation of the C system.
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yumi
Y
Sei
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan. Electronic address: mmizu@med.nagoya-u.ac.jp.
Masashi
M
Mizuno
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yasuhiro
Y
Suzuki
Immunology, Nagoya City University Graduate School of Medicine, Nagoya, Japan.
Masaki
M
Imai
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Keiko
K
Higashide
Complement Biology Group, Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
Claire L
CL
Harris
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Fumiko
F
Sakata
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Daiki
D
Iguchi
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Michitaka
M
Fujiwara
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yasuhiro
Y
Kodera
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Shoichi
S
Maruyama
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Seiichi
S
Matsuo
Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yasuhiko
Y
Ito
D018394:CA-125 Antigen / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology*; D018957:CD59 Antigens / Q000276:immunology*; D003167:Complement Activation; D003165:Complement System Proteins / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology* / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D008297:Male; D051916:Membrane Cofactor Protein / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D010530:Peritoneal Dialysis
D018394:CA-125 Antigen; C497857:CD46 protein, human; D019002:CD56 Antigen; D018957:CD59 Antigens; C508600:IL6 protein, human; D015850:Interleukin-6; C451759:MUC16 protein, human; D051916:Membrane Cofactor Protein; D008565:Membrane Proteins; C522918:NCAM1 protein, human; C051590:CD59 protein, human; D003165:Complement System Proteins
CD55; Complement; D/P creatinine; Membrane complement regulators; Peritoneal dialysis