Biorefining strategy for maximal monosaccharide recovery from three different feedstocks: eucalyptus residues, wheat straw and olive tree pruning.
This work proposes the biorefining of eucalyptus residues (ER), wheat straw (WS) and olive tree pruning (OP) combining hydrothermal pretreatment (autohydrolysis) with acid post-hydrolysis of the liquid fraction and enzymatic hydrolysis of the solid fraction towards maximal recovery of monosaccharides from those lignocellulose materials. Autohydrolysis of ER, WS and OP was performed under non-isothermal conditions (195-230°C) and the non-cellulosic saccharides were recovered in the liquid fraction while cellulose and lignin remained in the solid fraction. The acid post-hydrolysis of the soluble oligosaccharides was studied by optimizing sulfuric acid concentration (1-4%w/w) and reaction time (10-60 min), employing a factorial (2(2)) experimental design. The solids resulting from pretreatment were submitted to enzymatic hydrolysis by applying commercial cellulolytic enzymes Celluclast® 1.5L and Novozyme® 188 (0.225 and 0.025 g/g solid, respectively). This strategy provides high total monosaccharide recovery or high glucose recovery from lignocellulosic materials, depending on the autohydrolysis conditions applied.
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal; Instituto Superior de Agronomia (ISA), Universidade de Lisboa, Linking Landscape, Environment, Agriculture and Food (LEAF), Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Talita
T
Silva-Fernandes
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Luís Chorão
LC
Duarte
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Florbela
F
Carvalheiro
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Susana
S
Marques
Instituto Superior de Agronomia (ISA), Universidade de Lisboa, Linking Landscape, Environment, Agriculture and Food (LEAF), Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Maria Conceição
MC
Loureiro-Dias
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. Electronic address: cesar.fonseca@lneg.pt.
César
C
Fonseca
Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Francisco
F
Gírio
D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D005591:Chemical Fractionation; D005052:Eucalyptus / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D007700:Kinetics; D008031:Lignin / Q000737:chemistry; D009005:Monosaccharides / Q000302:isolation & purification*; D031658:Olea / Q000737:chemistry*; D012044:Regression Analysis; D014197:Trees / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products; D014994:Xylose / Q000032:analysis
D002241:Carbohydrates; D009005:Monosaccharides; D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D014994:Xylose; D005947:Glucose
Autohydrolysis; Chemical post-hydrolysis; Enzymatic hydrolysis; Lignocellulosic materials; Sugar-based biorefinery