H3 receptor renal expression in normal and diabetic rats.
BACKGROUND
To extend our previous observation of H4R upregulation in the kidney of diabetic rats, we evaluated in the same specimens the presence of the H3R.


METHODS
Kidney specimens from 24 8-week-old male Wistar rats (12 non-diabetic and 12 diabetic animals) were processed for both immunohistochemical and immunofluorescence analyses.


CONCLUSIONS
H3R is expressed in the apical membrane by collecting duct cells in the kidney of rats and it is significantly increased in diabetic animals. These data support the hypothesis that H3R could also mediate non-neuronal histamine effects, suggesting its involvement in fluid homeostasis.
Dipartimento di Medicina Sperimentale e Clinica Sezione di Anatomia e Istologia Università degli Studi di Firenze, Largo Brambilla 3, 50134, Florence, Italy.
Alessandro
A
Pini
Paul L
PL
Chazot
Eleonora
E
Veglia
Aldo
A
Moggio
Arianna Carolina
AC
Rosa
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018100:Receptors, Histamine H3 / Q000096:biosynthesis*
D018100:Receptors, Histamine H3