Characterizing novel thermophilic amylase producing bacteria from taptapani hot spring, odisha, India.
BACKGROUND
Amylases play a vital role in biotechnological studies and rank an important position in the world enzyme market (25% to 33%). Bioprocess method of amylase production is more effective than the other sources, since the technique is easy, cost effective, fast, and the enzymes of required properties can be procured.


OBJECTIVE
The current study aimed to report the characteristics of novel amylase producing bacterial strains isolated from Taptapani hot spring, Odisha, India.


METHODS
Bacterial strains were isolated by dilution plating method from the water samples collected from Taptapani Hot Spring, Odisha and screened for amylase production through starch hydrolysis. The bacterial isolates were identified morphologically, biochemically, and finally by 16S rDNA profiling.


RESULTS
Based on the morphological, physiological, biochemical characteristics and the molecular characterization, the isolates SS1, SS2, and SS3 were identified as Bacillus barbaricus, Aeromonas veroni, and Stenotrophomonas maltophilia, respectively. The approximate molecular weight of enzymes from SS1, SS2, and SS3 strains were 19 kDa, 56 kDa and 49 kDa, respectively.


CONCLUSIONS
The current report isolates, characterizes, and demonstrates the novel heat-adapted amylase-producing bacteria SS1, SS2 and SS3 from Taptapani hot spring, indicating its potentiality and stability under acidic conditions.
Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India.
Sudip Kumar
SK
Sen
Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India.
Sangeeta
S
Raut
Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India.
Soumya
S
Satpathy
Department of Biotechnology, National Institute of Technology, Warangal, Andhra Pradesh, India.
Prangya Ranjan
PR
Rout
Department of Biotechnology, Oriental Institute of Science and Technology, Midnapore, West Bengal, India.
Bidyut
B
Bandyopadhyay
Department of Microbiology, Vidyasagar University, Midnapore, West Bengal, India.
Pradeep Kumar
PK
Das Mohapatra
Alpha-amylase; Hot spring; Polysaccharides