Development of magnetic separation and quantum dots labeled immunoassay for the detection of mercury in biological samples.
A rapid and sensitive immunoassays of mercury (Hg) in biological samples was developed using quantum dots (QDs) and magnetic beads (MBs) as fluorescent and separated probes, respectively. A monoclonal antibody (mAb) that recognizes an Hg detection antigen (BSA-DTPA-Hg) complex was produced by the injection of BALB/c mice with an Hg immunizing antigen (KLH-DTPA-Hg). Then the ascites monoclonal antibodies were purified. The Hg monoclonal antibody (Hg-mAb) is conjugated with MBs to separate Hg from biological samples, and the other antibody, which is associated with QDs, is used to detect the fluorescence. The Hg in biological samples can be quantified using the relationship between the QDs fluorescence intensity and the concentration of Hg in biological samples following magnetic separation. In this method, the detection linear range is 1-1000ng/mL, and the minimum detection limit is 1ng/mL. The standard addition recovery rate was 94.70-101.18%. The relative standard deviation values were 2.76-7.56%. Furthermore, the Hg concentration can be detected in less than 30min, the significant interference of other heavy metals can be avoided, and the simultaneous testing of 96 samples can be performed. These results indicate that the method could be used for rapid monitoring Hg in the body.
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Hubo
H
Sun
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Mengmeng
M
Wang
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Jilong
J
Wang
Medical Experiment and Test Center, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Mi
M
Tian
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Hui
H
Wang
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China.
Zhiwei
Z
Sun
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing 100069, China. Electronic address: huangpl@ccmu.edu.cn.
Peili
P
Huang
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002417:Cattle; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006825:Hybridomas; D007118:Immunoassay / Q000379:methods*; D060328:Magnetic Phenomena; D008628:Mercury / Q000652:urine*; D008807:Mice, Inbred BALB C; D004369:Pentetic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D045663:Quantum Dots / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; C029560:bovine serum albumin-DTPA complex; D012710:Serum Albumin, Bovine; D004369:Pentetic Acid; D008628:Mercury
Magnetic beads; Mercury; Monoclonal antibody; Quantum dots