Lipid metabolism in response to individual short chain fatty acids during mixotrophic mode of microalgal cultivation: Influence on biodiesel saturation and protein profile.
Critical influence of different short chain fatty acids as organic carbon source, during growth (GP) and nutrient stress lipogenic phase (NSLP) was investigated on biomass and lipid productivity, in mixotrophic fed-batch microalgae cultivation. Nutrient deprivation induced physiological stress stimulated highest lipid productivity with acetate (total/neutral lipids, 35/17) with saturation index of 80.53% by the end of NSLP followed by butyrate (12/7%; 78%). Biomass growth followed the order of acetate (2.23 g/l) >butyrate (0.99 g/l) >propionate (0.77 g/l). VFA removal (as COD) was maximum with acetate (87%) followed by butyrate (55.09%) and propionate (10.60%). Palmitic acid was the most dominant fatty acid found in the fatty acid composition of all variants and butyrate fed system yielded a maximum of 44% palmitic acid. Protein profiling illustrated prominence of acetyl CoA-synthetase activity in acetate system. Thus, fatty acids provide a promising alternative feedstock for biodiesel production with integrated microalgae-biorefinery.
Academy of Scientific and Innovative Research, India.
Rashmi
R
Chandra
Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
Somya
S
Arora
Academy of Scientific and Innovative Research, India.
M V
MV
Rohit
Academy of Scientific and Innovative Research, India; Bioengineering and Environmental Sciences (BEES), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India. Electronic address: vmohan_s@yahoo.com.
S
S
Venkata Mohan
D000106:Acetate-CoA Ligase / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D001709:Biotechnology; D002087:Butyrates / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D000077194:Chlorophyll A; D005232:Fatty Acids, Volatile / Q000737:chemistry*; D005742:Gasoline; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000378:metabolism*; D009566:Nitrates / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011422:Propionates / Q000737:chemistry; D062065:Wastewater; D018508:Water Purification / Q000379:methods
D000106:Acetate-CoA Ligase; D000085:Acetates; D056804:Biofuels; D002087:Butyrates; D002734:Chlorophyll; D000077194:Chlorophyll A; D005232:Fatty Acids, Volatile; D005742:Gasoline; D008055:Lipids; D009566:Nitrates; D019308:Palmitic Acid; D010710:Phosphates; D011422:Propionates; D062065:Wastewater; C037184:chlorophyll b
Biodiesel; Biohydrogen; Palmitic acid; SDS–PAGE; Volatile fatty acids