microRNA-181a enhances cell proliferation in acute lymphoblastic leukemia by targeting EGR1.
Acute lymphoblastic leukemia (ALL) is an aggressive cancer that occurs in both children and adults. Starting from an integrated analysis of miRNA/mRNA expression profiles in 20 ALL patients, we identify a negative correlation between miR-181a and EGR1. Coherently, miR-181a over-expression in Jurkat T-ALL cells decreases EGR1 expression, increasing cell proliferation and enhancing the cell-cycle progression from G1 to S phase. We show that EGR1 is a new direct target of miR-181a. Our findings suggest that miR-181a behaves as an onco-miRNA in ALL by down-regulating EGR1.
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Lorena
L
Verduci
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Gianluca
G
Azzalin
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Silvia
S
Gioiosa
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Claudia
C
Carissimi
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Ilaria
I
Laudadio
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy. Electronic address: valerio.fulci@uniroma1.it.
Valerio
V
Fulci
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Giuseppe
G
Macino
D000328:Adult; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
Acute lymphoblastic leukemia (ALL); Cell cycle; Cell proliferation; EGR1; miR-181a; onco-miRNA