Chemiluminescent detection of enzymatically produced H2S.
Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assist other investigators with use of these chemical tools. We highlight the use of these tools use by displaying their selectivity and high sensitivity toward H2S and provide examples of assays we have developed to detect enzymatically produced H2S.
Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, Oregon, USA.
T
T
Spencer Bailey
Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, Oregon, USA. Electronic address: pluth@uoregon.edu.
Michael D
MD
Pluth
D000818:Animals; D002460:Cell Line; D003542:Cystathionine gamma-Lyase / Q000737:chemistry / Q000378:metabolism; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry*; D055668:Photochemical Processes; D051381:Rats
D005456:Fluorescent Dyes; D008165:Luminol; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
Chemiluminescence; Hydrogen sulfide; Luminescence; Luminol