High-throughput RNA profiling via up-front sample parallelization.
We describe a method called modular, early-tagged amplification (META) RNA profiling that can quantify a broad panel of microRNAs or mRNAs simultaneously across many samples and requires far less sequence depth than existing digital profiling technologies. The method assigns quantitative tags during reverse transcription to permit up-front sample pooling before competitive amplification and deep sequencing. This simple, scalable and inexpensive approach improves the practicality of large-scale gene expression studies.
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Azeet
A
Narayan
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Ananth
A
Bommakanti
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Abhijit A
AA
Patel
D002460:Cell Line; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D012313:RNA / Q000097:blood / Q000235:genetics* / Q000528:radiation effects
D012313:RNA