The effect of serum, intestinal and peritoneal visfatin levels on early diagnosis of acute mesenteric ischemia.
BACKGROUND
Acute mesenteric ischemia (AMI) is a rapidly progressive disease where early diagnosis is life-saving. As a new cytokine, levels of thevisfatin might be affected during the ischema and reperfusion. In our study, we obtained changes of visfatin levels in the serum, peritoneal and intestinal lavage samples in rats, to investigate the effectiveness of these changes in the early diagnosis of AMI.


METHODS
In group 1 (Sham group) the intestine was exteriorated after the laparotomy was performed and allowed to stand for 3 hours without ischemia. In group 2 (acute mesenteric ischemia-reperfusion group) the mesenteric artery was ligated and, mesenteric blood flow was restored after 60-minute ischemia. To compare with intestinal injury, in group 3 (acute pancreatitis group) the ductus pancreaticus was ligated, and the abdomen was closed for 3 days in expectation of the formation of pancreatitis. In all of the groups, the intestinal lavage, peritoneal lavage and blood samples were analyzed to evaluate the levels of visfatin, TNF-alpha, IL-6 and IL-8. Samples were taken before the procedure in all groups; additionally 60 minutes after ischemia and 120 minutes after reperfusion in group 2; and after the development of the pancreatitis in group 3.


RESULTS
Serum, intestinal and peritoneal lavage visfatin levels were found to be increased in group 2 and group 3 (P<0.05). In group 2, while serum TNF-alpha levels were increased in both ischemia and reperfusion; in intestinal lavage sample the increase was only in the ischemic phase (P<0.05). In group 2, IL-8 levels were significantly increased after ischemia in serum (P=0.03) and after reperfusion in intestinal lavage (P=0.004) samples.


CONCLUSIONS
Serum, intestinal and peritoneal visfatin levels were increased not only in the case of mesenteric ischemia, but also in acute pancreatitis. In these two clinical pathologies, the visfatin levels of the intestinal and peritoneal cavitiesmay increase parallel to the serum visfatin levels.
GATA Haydarpasa Training Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey - dralperucak@gmail.com.
Alper
A
Ucak
Veysel
V
Temizkan
Huseyin
H
Sen
Erman C
EC
Bulut
Murat F
MF
Can
Murat
M
Ugur
Arif
A
Selcuk
Zafer
Z
Kucukodaci
Ömer
Ö
Ozcan
D000208:Acute Disease; D000818:Animals; D016207:Cytokines / Q000737:chemistry*; D004195:Disease Models, Animal; D042241:Early Diagnosis; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D065666:Mesenteric Ischemia / Q000175:diagnosis*; D054409:Nicotinamide Phosphoribosyltransferase / Q000737:chemistry*; D010195:Pancreatitis / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C504970:nicotinamide phosphoribosyltransferase, rat