Afzelin attenuates asthma phenotypes by downregulation of GATA3 in a murine model of asthma.
Asthma is a serious health problem causing significant mortality and morbidity globally. Persistent airway inflammation, airway hyperresponsiveness, increased immunoglobulin E (IgE) levels and mucus hypersecretion are key characteristics of the condition. Asthma is mediated via a dominant T-helper 2 (Th2) immune response, causing enhanced expression of Th2 cytokines. These cytokines are responsible for the various pathological changes associated with allergic asthma. To investigate the anti-asthmatic potential of afzelin, as well as the underlying mechanisms involved, its anti-asthmatic potential were investigated in a murine model of asthma. In the present study, BALB/c mice were systemically sensitized using ovalbumin (OVA) followed by aerosol allergen challenges. The effect of afzelin on airway hyperresponsiveness, eosinophilic infiltration, Th2 cytokine and OVA‑specific IgE production in a mouse model of asthma were investigated. It was found that afzelin‑treated groups suppressed eosinophil infiltration, allergic airway inflammation, airway hyperresponsiveness, OVA-specific IgE and Th2 cytokine secretion. The results of the present study suggested that the therapeutic mechanism by which afzelin effectively treats asthma is based on reduction of Th2 cytokine via inhibition of GATA-binding protein 3 transcription factor, which is the master regulator of Th2 cytokine differentiation and production.
Department of Respiratory Diseases, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
Wenbo
W
Zhou
Department of Respiratory Diseases, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
Xiuhong
X
Nie
D000485:Allergens / Q000633:toxicity; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D016535:Bronchial Hyperreactivity / Q000235:genetics* / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004804:Eosinophils / Q000378:metabolism* / Q000473:pathology; D050990:GATA3 Transcription Factor / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008362:Mannosides / Q000008:administration & dosage*; D051379:Mice; D010047:Ovalbumin / Q000008:administration & dosage; D044945:Proanthocyanidins / Q000008:administration & dosage*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
D000485:Allergens; D016207:Cytokines; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D008362:Mannosides; D044945:Proanthocyanidins; D007073:Immunoglobulin E; C477954:afzelin; D010047:Ovalbumin