Inhalation exposure model of hydrogen sulfide (H₂S)-induced hypometabolism in the male Sprague-Dawley rat.
Hydrogen sulfide (H2S) has been accepted as a physiologically relevant cell-signaling molecule with both toxic and beneficial effects depending on its concentration in mammalian tissues. Notably, exposure to H2S in breathable air has been shown to decrease aerobic metabolism and induce a reversible hypometabolic-like state in laboratory rodent models. Herein, we describe an experimental exposure setup that can be used to define the reversible cardiovascular and metabolic physiology of rodents (rats) during H2S-induced hypometabolism and following recovery.
Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA; Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Asaf
A
Stein
Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA; Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
David W
DW
Kraus
Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA; Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Jeannette E
JE
Doeller
Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA; Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA; Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA. Electronic address: sbailey@uab.edu.
Shannon M
SM
Bailey
D000280:Administration, Inhalation; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000008:administration & dosage* / Q000097:blood; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010092:Oximetry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D056152:Respiratory Rate / Q000187:drug effects; D015398:Signal Transduction
D006454:Hemoglobins; D000410:Alanine Transaminase; D003402:Creatine Kinase; D006862:Hydrogen Sulfide
Heart; Hydrogen sulfide; Hypometabolism; Inhalation exposure model; Lungs; Mitochondria; Oxygen tension; Rats