[Degradation of polyribonucleotides: biocatalysis and the monitoring of products].
Macroporous monolithic material containing covalently linked ribonuclease A was used to create high-performance flow heterogeneous biocatalysts (bioreactors). The kinetic parameters of the degradation of polycytidylic acid were identified, and the properties of the obtained systems were compared. A HPLC method has been developed for monitoring products of biocatalytic degradation of RNA, and the possibility of using biocatalytic and HPLC columns in RNA degradation processes in a multicomponent mixture of biological molecules was shown.
E G
EG
Vlakh
M V
MV
Volokitina
D O
DO
Vinokhodov
T B
TB
Tennikova
D000818:Animals; D055162:Biocatalysis; D019149:Bioreactors; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D011066:Poly C / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D059367:RNA Cleavage; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*
D004800:Enzymes, Immobilized; D008689:Methacrylates; D011066:Poly C; D012313:RNA; D012259:Ribonuclease, Pancreatic