Simultaneous Visualization of Multiple mRNAs and Matrix Metalloproteinases in Living Cells Using a Fluorescence Nanoprobe.
Simultaneous monitoring of multiple tumour markers is of great significance for improving the accuracy of early cancer detection. In this study, a fluorescence nanoprobe has been prepared that can simultaneously monitor and visualize multiple mRNAs and matrix metalloproteinases (MMPs) in living cells. Confocal fluorescence imaging results indicate that the nanoprobe could effectively distinguish between cancer cells and normal cells even if one tumour maker of normal cells was overexpressed. Furthermore, it can detect changes in the expression levels of mRNAs and MMPs in living cells. The current approach could provide new tools for early cancer detection and monitoring the changes in expression levels of biomarkers during tumour progression.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals Shandong Normal University, Jinan 250014 (P.R. China).
Wei
W
Pan
Huijun
H
Yang
Na
N
Li
Limin
L
Yang
Bo
B
Tang
D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000032:analysis*; D009369:Neoplasms / Q000175:diagnosis; D061848:Optical Imaging; D012333:RNA, Messenger / Q000032:analysis*
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D020782:Matrix Metalloproteinases
analytical methods; imaging agents; mRNA; matrix metalloproteinases; nanoprobes