YKL-40 level in gingival crevicular fluid from patients with periodontitis and type 2 diabetes.
OBJECTIVE
YKL-40 is a chitin-binding glycoprotein, the level of which increases in inflammatory diseases, diabetes mellitus (DM), cardiovascular diseases, and tumors. Gingival crevicular fluid (GCF) contains many proteins and markers of periodontitis. The purpose of this study was to investigate YKL-40 level in GCF from patients with periodontitis and DM and the association between YKL-40 level and chronic periodontitis (CP) or DM.


METHODS
The subjects were 121 patients with DM, CP, DM and periodontitis (DM-P), and healthy subjects (H). GCF was collected using paper strips after the sites for GCF collection were clinically evaluated for probing depth (PD), gingival index (GI), and bleeding on probing (BOP). YKL-40 in GCF was identified by Western blotting, and its level was determined by ELISA.


RESULTS
YKL-40 was contained in GCF samples from H, DM, CP, and DM-P sites, and its levels (amount and concentration) in CP and DM-P were significantly higher than those in H and DM. GCF YKL-40 level significantly correlated with PD and GI, and its level in BOP-positive sites was significantly higher than that in BOP-negative ones.


CONCLUSIONS
GCF YKL-40 level was elevated in periodontitis, but not DM. YKL-40 in GCF may be an inflammatory marker for periodontitis.
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
J
J
Kido
Clinical Research Center for Diabetes, Tokushima University Hospital, Tokushima, Japan.
Y
Y
Bando
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
M
M
Bando
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
Y
Y
Kajiura
Clinical Research Center for Diabetes, Tokushima University Hospital, Tokushima, Japan.
Y
Y
Hiroshima
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
Y
Y
Inagaki
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
H
H
Murata
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
T
T
Ikuta
Department of Anatomy and Cell Biology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
R
R
Kido
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
K
K
Naruishi
Clinical Research Center for Diabetes, Tokushima University Hospital, Tokushima, Japan.
M
M
Funaki
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
T
T
Nagata
D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D016022:Case-Control Studies; D000071451:Chitinase-3-Like Protein 1 / Q000097:blood / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000097:blood / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000378:metabolism; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000378:metabolism; D010518:Periodontitis / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*
D015415:Biomarkers; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1
YKL-40; diabetes; gingival crevicular fluid; periodontitis