Optimisation of high-pressure ultrasonic-assisted extraction and antioxidant capacity of polysaccharides from the rhizome of Ligusticum chuanxiong.
High-pressure ultrasound-assisted extraction was used to extract polysaccharides from the Ligusticum chuanxiong rhizomes. Three independent variables, namely, extraction temperature (X1), ultrasonic power (X2) and extraction time (X3) were investigated. Response surface methodology was performed based on the results of single-factor tests. Experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and results were examined using appropriate statistical methods. The optimal conditions were as follows: extraction temperature of 85°C, ultrasonic power of 187 W and extraction time of 29 min. Under these conditions, the experimental yield of polysaccharides was 5.33%, which is close to the predicted yield of 5.41%. The extracted and purified polysaccharides showed excellent antioxidative effects on 2,2-diphenyl-1-picryl-hydrazy, hydroxyl and superoxide radicals in vitro.
College of Agronomy, Sichuan Agricultural University, Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of PR China, Chengdu 611130, China; Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Jin-Liang
JL
Liu
College of Agronomy, Sichuan Agricultural University, Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of PR China, Chengdu 611130, China.
Shun-Lin
SL
Zheng
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Qiao-Jia
QJ
Fan
College of Agronomy, Sichuan Agricultural University, Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of PR China, Chengdu 611130, China. Electronic address: yuanjichao5@163.com.
Ji-Chao
JC
Yuan
College of Agronomy, Sichuan Agricultural University, Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of PR China, Chengdu 611130, China.
Shi-Min
SM
Yang
College of Agronomy, Sichuan Agricultural University, Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of PR China, Chengdu 611130, China.
Fan-Lei
FL
Kong
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005591:Chemical Fractionation / Q000379:methods*; D005609:Free Radicals / Q000037:antagonists & inhibitors; D030004:Ligusticum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011312:Pressure; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D000069453:Ultrasonic Waves
D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D011134:Polysaccharides
Antioxidant; High-pressure ultrasonic-assisted extraction; Ligusticum chuanxiong; Polysaccharides; Response surface methodology