Fast determination of multiple-reaction intermediates for long-chain dicarboxylic Acid biotransformation by gas chromatography-flame ionization detector.
For the analysis of multiple-reaction intermediates for long-chain dicarboxylic acid biotransformation, simple and reproducible methods of extraction and derivatization were developed on the basis of gas chromatography with flame ionization detector (GC-FID) instead of mass spectrometry. In the derivatization step, change of the ratio of pyridine to MSTFA from 1:3 to 9:1 resulted in higher peak intensity (p = 0.021) and reproducibility (0.6%CV) when analyzing 32 g/l ricinoleic acid (RA). Extraction of RA and ω-hydroxyundec- 9-enoic acid with water containing 100 mM Tween 80 showed 90.4-99.9% relative extraction efficiency and 2-7%CV compared with those with hydrophobic ethyl acetate. In conclusion, reduction of the pyridine content and change of the extraction solvent to water with Tween 80 provided compatible derivatization and extraction methods to GC-FID-based analysis of longchain carboxylic acids.
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Yong-Han
YH
Cho
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Hye-Jin
HJ
Lee
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Jung-Eun
JE
Lee
Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea.
Soo-Jung
SJ
Kim
Department of Bio and Chemical Engineering, Hongik University, Sejong 339-701, Republic of Korea.
Kyungmoon
K
Park
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Do Yup
DY
Lee
Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
Yong-Cheol
YC
Park
D003998:Dicarboxylic Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D005410:Flame Ionization / Q000379:methods*; D011725:Pyridines / Q000378:metabolism; D012277:Ricinoleic Acids / Q000378:metabolism; D014476:Undecylenic Acids / Q000378:metabolism
D003998:Dicarboxylic Acids; D005231:Fatty Acids, Unsaturated; D011725:Pyridines; D012277:Ricinoleic Acids; D014476:Undecylenic Acids; C000590836:omega-hydroxyundec-9-enoic acid; C030521:ricinoleic acid; C023666:pyridine