[Isolation and physiological and biochemical characterization of the Chlamydomonas reinhardtii C-41 mutant, a superproducer of ξ-carotene].
The composition of the carotenes and xanthophylls of Chlamydomonas reinhardtii Dang. C-41, a mutant of a unicellular green alga and a superproducer of ξ-carotene, was studied. The light-harvesting complexes and a complex of the PS-II reaction center were established to be disrupted in the C-41 mutant. However, the mutant retained a high (up to 46%) photosynthetic activity and the capacity to accumulate chlorophylls and carotenoids (up to 50%). The composition of carotenes was studied, and it was shown that, in contrast to wild-type K(+) cells, which accumulate up to 95% of β13-carotene and 5% α-carotene, cells of the C-41 mutant contained 43% β13-carotene, 19% β3-zeacarotene, and 38% ξ-carotene. The high level of C-41 mutant biomass accumulation made it possible to recommend the mutant as a superproducer of ξ-carotene in phytobiotechnology.
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D002338:Carotenoids / Q000096:biosynthesis* / Q000302:isolation & purification; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002734:Chlorophyll / Q000096:biosynthesis; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression; D045342:Light-Harvesting Protein Complexes / Q000235:genetics* / Q000378:metabolism; D000077276:Lycopene; D009154:Mutation; D045332:Photosystem II Protein Complex / Q000235:genetics* / Q000378:metabolism; D024341:Xanthophylls / Q000096:biosynthesis; D019207:beta Carotene / Q000096:biosynthesis / Q000302:isolation & purification
D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D024341:Xanthophylls; D019207:beta Carotene; D002734:Chlorophyll; D002338:Carotenoids; C041635:alpha-carotene; C030657:zeacarotene; D000077276:Lycopene