Development of a hybridoma cell line secreting monoclonal antibody against S protein of a Chinese variant of PEDV.
Gene encoding 22-380 aa of spike protein of porcine epidemic diarrhea virus (PEDV) isolate in China was cloned and expressed as a recombinant protein in Escherichia coli BL21(DE3). Female BALB/c mice were immunized with the purified recombinant S protein, and a monoclonal antibody (MAb) designated as 2D1 against S protein was achieved by hybridoma technique. MAb 2D1 reacted with S protein of PEDV specifically. The monoclonal antibody 2D1 may provide a useful tool as a specific diagnostic reagent for detecting S protein of the Chinese variant of PEDV and for further investigation into the virus' pathogenic mechanism.
College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou, China .
Ximei
X
Lei
Jun
J
Zhao
Xiaomeng
X
Wang
Yongxiang
Y
Zhao
Chuanqing
C
Wang
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D002460:Cell Line; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D018703:Founder Effect; D015870:Gene Expression; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053485:Porcine epidemic diarrhea virus / Q000276:immunology / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D064370:Spike Glycoprotein, Coronavirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus