Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol.
The growth, lipid production, and carbon use efficiency of two oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, were compared under nitrogen-limiting conditions with glycerol as the carbon source. Final biomass concentrations of R. babjevae and R. diobovatum were 9.4±0.80g/L and 12.0±0.82g/L, respectively, after 120h of growth. Final lipid accumulation in for R. babjevae and R. diobovatum were 34.9±3.0% cell dry weight (cdw) and 63.7±4.5% cdw, respectively. Biomass production increased to 9.9±0.2g/L for R. babjevae and 14.1±0.2g/L for R. diobovatum in medium containing biodiesel-derived waste glycerol, but lipid accumulation decreased in both species. In a 7L bioreactor with biodiesel-derived glycerol as carbon source, R. diobovatum produced 13.6±0.4g/L biomass and accumulated lipids to 50.7±2.2% cdw, matching flask experiments.
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: garretmunch@gmail.com.
Garret
G
Munch
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: rsestric@gmail.com.
Ryan
R
Sestric
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: Richard.Sparling@umanitoba.ca.
Richard
R
Sparling
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: david.levin@umanitoba.ca.
David B
DB
Levin
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: nazim.cicek@umanitoba.ca.
Nazim
N
Cicek
D001487:Basidiomycota / Q000145:classification / Q000502:physiology*; D061249:Batch Cell Culture Techniques / Q000379:methods; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D005990:Glycerol / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control*; D050356:Lipid Metabolism / Q000502:physiology*; D013045:Species Specificity
D056804:Biofuels; D007220:Industrial Waste; D005990:Glycerol
Biodiesel; Biodiesel-derived waste glycerol; Oleaginous yeasts; Rhodosporidium; Single-cell oil