[Formation of trans-1,3-pentadiene in off-flavor food].
The purpose of the present study is to investigate the production of trans-1,3-pentadiene in a sorbic acid-containing food which was the subject of a complaint that it was off-flavor. Penicillium sp. was isolated from the off-flavor food. The isolated Penicillium sp. was identified as Penicillium chrysogenum by DNA sequencing of the internal transcribed spacer region and the D1/D2 region of the 28S subunit. When P. chrysogenum was cultured in the presence of potassium sorbate, trans-1,3-pentadiene was produced and detected by GC-MS after solid-phase micro extraction. The production of trans-1,3-pentadiene by P. chrysogenum in the culture solution was pH-dependent. These results suggest that the production of trans-1,3-pentadiene in the off-flavor food was mainly due to the decomposition of sorbic acid by P. chrysogenum.
Saitama Prefectural Institute of Public Health.
Koichi
K
Imai
Aoi
A
Yoneda
Ikue
I
Osaka
Rie
R
Ishii
Mariko
M
Takano
D000466:Alkadienes / Q000032:analysis* / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D005503:Food Additives / Q000378:metabolism; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D010408:Penicillium chrysogenum / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010420:Pentanes / Q000032:analysis* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D052617:Solid Phase Microextraction; D013011:Sorbic Acid / Q000378:metabolism
D000466:Alkadienes; D004271:DNA, Fungal; D005503:Food Additives; D010420:Pentanes; C011970:1,3-pentadiene; D013011:Sorbic Acid