Nasal ectomesenchymal stem cells: multi-lineage differentiation and transformation effects on fibrin gels.
Ectomesenchymal stem cells (EMSCs) are novel adult stem cells derived from the cranial neural crest. However, their stemness and multi-lineage differentiation potential on three-dimensional fibrin gels has not yet been explored. The objective of this study was to investigate induced differentiation of EMSCs on fibrin gels and their remodeling effects on the scaffolds during the induced differentiation process. The results indicated that CD133(+)/nestin(+)/CD44(+) EMSCs were extensively distributed in the lamina propria of the nasal mucosa. The passaged cells could be induced to differentiate to a greater degree into neurons, Schwann cells and osteoblasts on three-dimensional fibrin gels than on two-dimensional glass slides. More importantly, the induced Schwann cells and osteoblasts exerted channelized and calcified remodeling effects, respectively, on the fibrin gels. Thus, these reshaped scaffolds have desirable biological properties, such as good cell adhesion, biocompatibility and guidance over the cell behavior, providing a tissue-committed niche for specific tissue generation.
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, PR China; Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China.
Zhijian
Z
Zhang
Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China; Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212001, PR China.
Qinghua
Q
He
Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China; Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212001, PR China.
Wenwen
W
Deng
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, PR China; Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China.
Qian
Q
Chen
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, PR China; Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China.
Xinyuan
X
Hu
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, PR China; Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China.
Aihua
A
Gong
Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China; Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212001, PR China.
Xia
X
Cao
Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China; Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212001, PR China.
Jiangnan
J
Yu
Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang 212001, PR China; Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212001, PR China. Electronic address: xmxu@ujs.edu.cn.
Ximing
X
Xu
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D005337:Fibrin / Q000494:pharmacology* / Q000648:ultrastructure; D005782:Gels / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009297:Nasal Mucosa / Q000166:cytology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011107:Polylysine / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects
D005782:Gels; D011107:Polylysine; D005337:Fibrin
Ectomesenchymal stem cells; Fibrin gel; Induced differentiation; Neural crest; Stemness; Transformation