Microreactor array device.
We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ~10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.
1] Engineering Arts LLC, Tempe, Arizona, U.S.A [2] The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Peter
P
Wiktor
Engineering Arts LLC, Tempe, Arizona, U.S.A.
Al
A
Brunner
Engineering Arts LLC, Tempe, Arizona, U.S.A.
Peter
P
Kahn
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Ji
J
Qiu
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Mitch
M
Magee
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Xiaofang
X
Bian
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Kailash
K
Karthikeyan
The Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona, U.S.A.
Joshua
J
LaBaer
D004247:DNA / Q000235:genetics*; D004867:Equipment Design; D020869:Gene Expression Profiling / Q000295:instrumentation*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation*; D015203:Reproducibility of Results
D020543:Proteome; D004247:DNA