Anticancer effect of a novel palladium-saccharinate complex of terpyridine by inducing apoptosis on Ehrlich ascites carcinoma (EAC) in Balb-C mice.
OBJECTIVE
[Pd(sac)(terpy)](sac)•4H2O (sac=saccharinate and terpy=2,2':6',2"-terpyridine) is newly-synthesized palladium(II) (Pd) complex. We investigated the antiproliferative and apoptotic effects of this complex on Ehrlich ascites carcinoma (EAC).


METHODS
EAC cells were administered to 33 Balb/c mice. Mice were divided randomly into four groups: control, cisplatin, Pd(II) complex and paclitaxel. Control group animals received 0.9% NaCl; other groups received treatments cisplatin, Pd(II) complex and paclitaxel on days 7 and 12. At day 14, animals were sacrificed. Expression of active caspase-3, p53 and proliferating cell nuclear antigen (PCNA) was investigated and apoptosis was evaluated by terminal deoxynucleotidyltransferase (TdT)-mediated nick-end labelling (TUNEL) technique.


RESULTS
Expression of p53 and PCNA were found to be decreased (p<0.0001), cells with active caspase-3 and TUNEL-positive cells were found to be increased (p<0.0001) in all treatment groups.


CONCLUSIONS
Like cisplatin and paclitaxel, this Pd(II) complex has a strong anticancer activity against EAC by inducing apoptosis and suppressing proliferation in vivo.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Elif Ilkay
EI
Ikitimur-Armutak
Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Kivilcim
K
Sonmez
Section of Zoology, Istanbul University, Istanbul, Turkey.
Kadriye
K
Akgun-Dar
Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Gulbin
G
Sennazli
Section of Botany, Department of Biology, Istanbul University, Istanbul, Turkey.
Aysegul
A
Kapucu
Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Funda
F
Yigit
Department of Inorganic Chemistry, Faculty of Chemistry, Uludag University, Bursa, Turkey.
Veysel Turan
VT
Yilmaz
Department of Biochemistry, Faculty of Medicine, Uludag University, Bursa, Turkey engin_ulukaya@yahoo.com.
Engin
E
Ulukaya
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D056831:Coordination Complexes / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010165:Palladium / Q000737:chemistry*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011725:Pyridines / Q000494:pharmacology*; D012439:Saccharin / Q000031:analogs & derivatives* / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D000970:Antineoplastic Agents; D056831:Coordination Complexes; D018809:Proliferating Cell Nuclear Antigen; D011725:Pyridines; D016159:Tumor Suppressor Protein p53; D010165:Palladium; D053148:Caspase 3; D012439:Saccharin
EAC; PCNA; Pd(II) complex; apoptosis; in vivo; tumor