BDP-30, a systemic resistance inducer from Boerhaavia diffusa L., suppresses TMV infection, and displays homology with ribosome-inactivating proteins.
Root extract of Boerhaavia diffusa L. induced systemic resistance in tobacco against Tobacco mosaic virus. A 30 kDa protein was isolated as the active component, called BDP-30 on the basis of the molecular weight and source plant. BDP-30, a glycoprotein, was found to be temperature and protease resistant. It was basic, possessing a pI greater than 9.0. In-gel proteolytic digestion of BDP-30 generated two peptides that possessed the amino acid sequence KLYDIPPLR and KVTLPYSGNYER by LC/MS/MS. Both peptides shared absolute sequence identity with trichosanthin, a ribosome-inactivating protein from Trichosanthes kirilowii, and a 78 percent and 100 percent homology respectively with an RIP from Bryonia dioica, bryodin. Further, effort was made to look at the fate of TMV in induced resistant Nicotiana tabacum cv. Xanthi, a systemic host of the virus, at specified days after inoculation in control and treated plants. TMV coat protein (CP) was detected by immunoblot 7 days post inoculation up to 21 days in the control set, but not in treated resistant plants. TMV RNA was detected by RT-PCR using TMV-CP specific primers. Resistant tobacco did not show presence of TMV RNA up to 21 days of inoculation. This suggests that BDP-30 may be suppressing TMV replication.
Molecular Plant Virology Lab, Department of Botany, Lucknow University, Lucknow 226 007, India.
Shalini
S
Srivastava
H N
HN
Verma
Aparana
A
Srivastava
Vivek
V
Prasad
D036022:Capsid Proteins / Q000302:isolation & purification; D060467:Disease Resistance / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D039281:Nyctaginaceae / Q000378:metabolism*; D010935:Plant Diseases / Q000517:prevention & control / Q000821:virology; D010936:Plant Extracts / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D054788:Ribosome Inactivating Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D036022:Capsid Proteins; D006023:Glycoproteins; C009496:H protein, Tobacco mosaic virus; D010936:Plant Extracts; D010940:Plant Proteins; D054788:Ribosome Inactivating Proteins