[Determination method of ultra-high-intensity sweetener, advantame, in processed foods by HPLC and LC-MS/MS].
A simple method using HPLC and LC-MS/MS was developed for the determination of ultra-high-intensity sweetener, advantame, in processed foods. Advantame was extracted by dialysis, and cleaned up on a Sep-Pak Plus C18 cartridge, then determined by HPLC and LC-MS/MS. The recoveries from 5 kinds of processed foods fortified at the levels of 0.001 g/kg and 0.01 g/kg were 64.1-89.9% (RSD 0.9-6.9%) by HPLC and 68.8-99.9% (RSD 0.8-4.9%) by LC-MS/MS. The quantitation limit was 0.0004 g/kg by HPLC and 0.00004 g/kg by LC-MS/MS.
Nagoya City Public Health Research Institute.
Miki
M
Kobayashi
Hisaya
H
Terada
Masahiro
M
Nakajima
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000032:analysis* / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods; D013549:Sweetening Agents / Q000032:analysis* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004151:Dipeptides; D013549:Sweetening Agents; C570172:N-(N-(3-(3-hydroxy-4-methoxyphenyl) propyl)-alpha-aspartyl)-L-phenylalanine 1-methyl ester