Protein imaging. Single-protein spin resonance spectroscopy under ambient conditions.
Magnetic resonance is essential in revealing the structure and dynamics of biomolecules. However, measuring the magnetic resonance spectrum of single biomolecules has remained an elusive goal. We demonstrate the detection of the electron spin resonance signal from a single spin-labeled protein under ambient conditions. As a sensor, we use a single nitrogen vacancy center in bulk diamond in close proximity to the protein. We measure the orientation of the spin label at the protein and detect the impact of protein motion on the spin label dynamics. In addition, we coherently drive the spin at the protein, which is a prerequisite for studies involving polarization of nuclear spins of the protein or detailed structure analysis of the protein itself.
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences. Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei 230026, China.
Fazhan
F
Shi
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences.
Qi
Q
Zhang
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences. Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei 230026, China.
Pengfei
P
Wang
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230000, China.
Hongbin
H
Sun
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230000, China.
Jiarong
J
Wang
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences. Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei 230026, China.
Xing
X
Rong
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences.
Ming
M
Chen
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences. Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei 230026, China.
Chenyong
C
Ju
3rd Physics and Integrated Quantum Science and Technology (IQST), University of Stuttgart, 70569 Stuttgart, Germany.
Friedemann
F
Reinhard
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230000, China.
Hongwei
H
Chen
3rd Physics and Integrated Quantum Science and Technology (IQST), University of Stuttgart, 70569 Stuttgart, Germany.
Jörg
J
Wrachtrup
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230000, China.
Junfeng
J
Wang
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei 230026, China. Joint Laboratory of Quantum Biophysics, USTC Institute of Biophysics and Chinese Academy of Sciences. Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei 230026, China.
Jiangfeng
J
Du
D018130:Diamond / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D064246:Mad2 Proteins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D057054:Molecular Imaging / Q000379:methods*; D009038:Motion; D009584:Nitrogen / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels
D064246:Mad2 Proteins; D011506:Proteins; D013113:Spin Labels; D011107:Polylysine; D018130:Diamond; D009584:Nitrogen