Development of novel and polymorphic microsatellite DNA loci from Haliotis ovina.
Twelve microsatellite loci were developed from Haliotis ovina by the magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from 3 wild populations. The number of alleles per locus ranged from 2 to 5, and the polymorphism information content ranged from 0.1228 to 0.6542. Observed and expected heterozygosities ranged from 0.0000 to 0.7778 and 0.1288 to 0.6310, respectively. These loci should provide useful information for genetic studies such as genetic diversity, pedigree analysis, construction of genetic linkage maps, and marker-assisted selection breeding in H. ovina.
Fisheries College, Jimei University, Xiamen, China lizhongbao@jmu.edu.cn.
Z B
ZB
Li
Fisheries College, Jimei University, Xiamen, China.
X J
XJ
Tian
Fisheries College, Jimei University, Xiamen, China.
J B
JB
Shangguan
Fisheries College, Jimei University, Xiamen, China.
Y Y
YY
Cao
Fisheries College, Jimei University, Xiamen, China.
G L
GL
Zhang
Fisheries College, Jimei University, Xiamen, China.
L N
LN
Chen
D000818:Animals; D004247:DNA / Q000235:genetics*; D049851:Gastropoda / Q000235:genetics*; D056426:Genetic Loci; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic
D005819:Genetic Markers; D004247:DNA