Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L.
Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Hossein
H
Mahmoudvand
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Fatemeh
F
Ezzatkhah
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Fariba
F
Sharififar
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Iraj
I
Sharifi
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Ebrahim Saedi
ES
Dezaki
D000080462:Acyclic Monoterpenes; D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000081008:Bicyclic Monoterpenes; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003511:Cyclohexanols / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D000077591:Eucalyptol; D020128:Inhibitory Concentration 50; D007895:Leishmania tropica / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D031651:Myrtus / Q000737:chemistry*; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
D000080462:Acyclic Monoterpenes; D000981:Antiprotozoal Agents; D000081008:Bicyclic Monoterpenes; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; C018584:linalool; C005451:alpha-pinene; D000077591:Eucalyptol
J774-A1 cell; Leishmania tropica; amastigote; cutaneous leishmaniasis; myrtle; promastigote