Adhesion, invasion, intracellular survival and cytotoxic activity of strains of Aeromonas spp. in HEp-2, Caco-2 and T-84 cell lines.
The genus Aeromonas contains important pathogen for both humans and other animals, being responsible for the etiology of intestinal and extraintestinal diseases. The pathology caused by these bacteria involves several virulence factors, such as the ability to produce toxins, adhesion and invasion. The properties conferred by these factors have been extensively studied in experiments of interaction between bacterial strains and cell culture. We evaluate the interaction of eight Aeromonas spp. strains, previously isolated from human faeces, food and water with HEp-2, Caco-2 and T-84 cell lines. Cytotoxic effects, the pattern of adhesion, invasive capacity and intracellular survival were analyzed. The results showed that Aeromonas strains were adherent to three cells lines in 6 h of incubation, displaying the aggregative adherence pattern. Among eight strains studied, 50% produced cytotoxic effects on HEp-2 cells, while none of the strains produced cytotoxic effects on Caco-2 and T-84 cells at 48 h. This study demonstrated that subsets of Aeromonas isolated from different sources were able to invade intestinal (T-84, Caco-2) and epithelial (HEp-2) cell lines cultivated in vitro surviving in intracellular environments up to 72 h. Finally, our results support the pathogenic potential of Aeromonas, especially those of food and clinical sources.
Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de setembro, 87- fundos, 3° andar, Vila Isabel, RJ, CEP 20551-030, Brazil.
Paula Azevedo
PA
dos Santos
Ana Claudia Machado
AC
Pereira
Andréa Fonseca
AF
Ferreira
Maria Angélica
MA
de Mattos Alves
Ana Cláudia Paula
AC
Rosa
Angela Corrêa
AC
Freitas-Almeida
D000333:Aeromonas / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002460:Cell Line; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005618:Fresh Water / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability; D014774:Virulence