A lower pH value benefits regeneration of Trichosanthes kirilowii by somatic embryogenesis, involving rhizoid tubers (RTBs), a novel structure.
A new approach was established for the regeneration of Trichosanthes kirilowii from root, stem, and leaf explants by somatic embryogenesis (SE), involving a previously unreported SE structure, rhizoid tubers (RTBs). During SE, special rhizoids were first induced from root, stem, and leaf explants with average rhizoid numbers of 62.33, 40.17, and 11.53 per explant, respectively, on Murashige and Skoog (MS) medium (pH 4.0) supplemented with 1.0 mg/L 1-naphthaleneacetic acid (NAA) under dark conditions. Further, one RTB was formed from each of the rhizoids on MS medium (pH 4.0) supplemented with 20 mg/L thidiazuron (TDZ) under light conditions. In the suitable range (pH 4.0-9.0), a lower pH value increased the induction of rhizoids and RTBs. Approximately 37.77, 33.47, and 31.07% of in vivo RTBs from root, stem, and leaf explants, respectively, spontaneously developed into multiple plantlets on the same MS medium (supplemented with 20 mg/L TDZ) for induction of RTBs, whereas >95.00% of in vitro RTBs from each kind of explant developed into multiple plantlets on MS medium supplemented with 5.0 mg/L 6-benzylaminopurine (BAP). Morphological and histological analyses revealed that RTB is a novel type of SE structure that develops from the cortex cells of rhizoids.
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Ke-dong
KD
Xu
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Yun-xia
YX
Chang
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Ju
J
Zhang
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Pei-long
PL
Wang
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Jian-xin
JX
Wu
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Yan-yan
YY
Li
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Xiao-wen
XW
Wang
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Wei
W
Wang
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Kun
K
Liu
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Yi
Y
Zhang
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
De-shui
DS
Yu
College of Life Science and Agronomy, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Li-bing
LB
Liao
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Yi
Y
Li
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Shu-ya
SY
Ma
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Guang-xuan
GX
Tan
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, East Wenchang Street, Zhoukou, 466001, People's Republic of China.
Cheng-wei
CW
Li
D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D012038:Regeneration; D029778:Trichosanthes / Q000254:growth & development / Q000502:physiology*