Non-derivatized glycan analysis by reverse phase liquid chromatography and ion mobility-mass spectrometry.
A simple method for the analysis of non-derivatized glycans using a reverse phase column on a liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instrument. The methodology supports both glycomic and proteomic work flows without the necessity of switching columns.
Department of Chemistry, Center for Innovative Technology, Institute of Chemical Biology, and Institute for Integrative Biosystems Research and Education, Vanderbilt University, 7330 Stevenson Center, Nashville, TN 37235, USA. john.a.mclean@vanderbilt.edu.
Nichole M
NM
Lareau
Jody C
JC
May
John A
JA
McLean
D056148:Chromatography, Reverse-Phase / Q000379:methods*; D054794:Glycomics; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
D011134:Polysaccharides