Different NaCl-induced calcium signatures in the Arabidopsis thaliana ecotypes Col-0 and C24.
A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.
Australian Centre for Plant Functional Genomics and The University of Adelaide, Adelaide, Australia; Centre for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Saudi Arabia.
Sandra M
SM
Schmöckel
Australian Centre for Plant Functional Genomics and The University of Adelaide, Adelaide, Australia; The Plant Accelerator, Australian Plant Phenomics Facility and The University of Adelaide, Adelaide, Australia.
Alexandre F
AF
Garcia
The Plant Accelerator, Australian Plant Phenomics Facility and The University of Adelaide, Adelaide, Australia.
Bettina
B
Berger
Centre for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Saudi Arabia.
Mark
M
Tester
Department of Plant Sciences, The University of Cambridge, Cambridge, United Kingdom.
Alex A R
AA
Webb
Australian Centre for Plant Functional Genomics and The University of Adelaide, Adelaide, Australia.
Stuart J
SJ
Roy
D000331:Aequorin / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D060146:Ecotype; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D012965:Sodium Chloride / Q000378:metabolism*
D029681:Arabidopsis Proteins; D012965:Sodium Chloride; D000331:Aequorin; D002118:Calcium