ENU mutagenesis in the mouse.
This article describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.
Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
Rolf
R
Stottmann
David
D
Beier
D000818:Animals; D005038:Ethylnitrosourea / Q000494:pharmacology*; D016678:Genome / Q000187:drug effects*; D006801:Humans; D051379:Mice / Q000235:genetics*; D023421:Models, Animal; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D010641:Phenotype
D009153:Mutagens; D005038:Ethylnitrosourea
alkylating agent; genome sequencing; per locus mutation frequency; phenotype-driven mutagenesis; spermatogonial stem cells