Dietary unsaturated fatty acids differently affect catecholamine handling by adrenal chromaffin cells.
Catecholamines (CA) play an important role in cardiovascular (CDV) disease risk. Namely, noradrenaline (NA) levels positively correlate whereas adrenaline (AD) levels negatively correlate with obesity and/or CDV disease. Western diets, which are tipically rich in Ω-6 fatty acids (FAs) and deficient in Ω-3 FAs, may contribute to the development of obesity, type 2 diabetes and/or coronary artery disease. Taking this into consideration and the fact that our group has already described that saturated FAs affect catecholamine handling by adrenal chromaffin cells, this work aimed to investigate the effect of unsaturated FAs upon catecholamine handling in the same model. Our results showed that chronic exposure to unsaturated FAs differently modulated CA cellular content and release, regardless of both FA series and number of carbon atoms. Namely, the Ω-6 arachidonic and linoleic acids, based on their effect on CA release and cellular content, seemed to impair NA and AD vesicular transport, whereas γ-linolenic acid selectively impaired AD synthesis and release. Within the Ω-9 FAs, oleic acid was devoid of effect, and elaidic acid behaved similarly to γ-linolenic acid. Eicosapentaenoic and docosahexaenoic acids (Ω-3 series) impaired the synthesis and release of both NA and AD. These results deserve attention and future development, namely, in what concerns the mechanisms involved and correlative effects in vivo.
Department of Biochemistry, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto.
Andreia
A
Gomes
Department of Biochemistry, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto.
Gustavo
G
Correia
Department of Biochemistry, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto.
Marisa
M
Coelho
Department of Biochemistry, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal.
João Ricardo
JR
Araújo
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal.
Maria João
MJ
Pinho
Molecular Oncology Group, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal; ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira n.° 228, 4050-313 Porto, Portugal.
Ana Luisa
AL
Teixeira
Molecular Oncology Group, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal; ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira n.° 228, 4050-313 Porto, Portugal; CEBIMED, Faculty of Health Sciences of Fernando Pessoa University, Praça 9 de Abril, n° 349, 4249-004 Porto, Portugal; LPCC, Research Department-Portuguese League Against Cancer (Núcleo Regional do Norte), Estrada Interior da Circunvalação, n° 6657, 4200-177 Porto, Portugal.
Rui
R
Medeiros
Department of Biochemistry, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal; Department of Medical Education and Simulation, Faculty of Medicine,University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto. Electronic address: lribeiro@med.up.pt.
Laura
L
Ribeiro
D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*
D002395:Catecholamines; D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated
Adrenaline; Noradrenaline; Phenylethanolamine N-methyltransferase; Tyrosine hydroxylase; Unsaturated lipids