[Elevated levels of dendritic cell-correlated miRNAs in ascites and sera of patients with ovarian cancer].
OBJECTIVE
To investigate the relationship between the clinical characteristics of ovarian cancer and the levels of miRNAs which could regulate the differentiation of dendritic cells, and assess its value in estimating the immune state and prognosis of ovarian cancer patients.


METHODS
Real-time quantitative PCR was used to determine the levels of miRNAs in ascites and sera of 39 cases of ovarian cancer and in the sera of 20 healthy women.


RESULTS
The levels of miR-21, miR-222 and miR-142-3p in sera of ovarian cancer patients were significantly higher than those of the healthy. The expression of miR-21 was higher in the advanced stages (III and IV) than in the earlier stages (I and II ), whereas the level of serum miR-142-3p was lower in high pathological grade than in low grade. The levels of miR-21 and miR-222 in the ascites were higher than those in the peripheral blood.


CONCLUSIONS
The increased expressions of miR-21, miR-222 and miR-142-3p in the ascites and sera of ovarian cancer patients might be correlated with the clinical pathological grading of the patients.
Department of Gynaecology and Obstetrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Xiaoqing
X
Wu
Department of Gynaecology and Obstetrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Xiaogai
X
Zhi
Department of Gynaecology and Obstetrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Mengna
M
Liu
Department of Gynaecology and Obstetrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Jingyan
J
Xie
Department of Gynaecology and Obstetrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Shuli
S
Zhao
D000368:Aged; D001201:Ascites / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D035683:MicroRNAs