Inter-epidemic acquisition of Rift Valley fever virus in humans in Tanzania.
BACKGROUND
In East Africa, epidemics of Rift Valley fever (RVF) occur in cycles of 5-15 years following unusually high rainfall. RVF transmission during inter-epidemic periods (IEP) generally passes undetected in absence of surveillance in mammalian hosts and vectors. We studied IEP transmission of RVF and evaluated the demographic, behavioural, occupational and spatial determinants of past RVF infection.


METHODS
Between March and August 2012 we collected blood samples, and administered a risk factor questionnaire among 606 inhabitants of 6 villages in the seasonally inundated Kilombero Valley, Tanzania. ELISA tests were used to detect RVFV IgM and IgG antibodies in serum samples. Risk factors were examined by mixed effects logistic regression.


RESULTS
RVF virus IgM antibodies, indicating recent RVFV acquisition, were detected in 16 participants, representing 2.6% overall and in 22.5% of inhibition ELISA positives (n = 71). Four of 16 (25.0%) IgM positives and 11/71 (15.5%) of individuals with inhibition ELISA sero-positivity reported they had had no previous contact with host animals. Sero-positivity on inhibition ELISA was 11.7% (95% CI 9.2-14.5) and risk was elevated with age (odds ratio (OR) 1.03 per year; 95% CI 1.01-1.04), among milkers (OR 2.19; 95% CI 1.23-3.91), and individuals eating raw meat (OR 4.17; 95% CI 1.18-14.66). Households keeping livestock had a higher probability of having members with evidence of past infection (OR = 3.04, 95% CI = 1.42-6.48) than those that do not keep livestock.


CONCLUSIONS
There is inter-epidemic acquisition of RVFV in Kilombero Valley inhabitants. In the wake of declining malaria incidence, these findings underscore the need for clinicians to consider RVF in the differential diagnosis for febrile illnesses. Several types of direct contact with livestock are important risk factors for past infection with RVFV in this study's population. However, at least part of RVFV transmission appears to have occurred through bites of infected mosquitoes.
Ifakara Health Institute, Ifakara, Tanzania; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium.
Robert David
RD
Sumaye
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Emmanuel Nji
EN
Abatih
Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium.
Etienne
E
Thiry
Ifakara Health Institute, Ifakara, Tanzania.
Mbaraka
M
Amuri
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Dirk
D
Berkvens
Ifakara Health Institute, Ifakara, Tanzania.
Eveline
E
Geubbels
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012295:Rift Valley Fever / Q000453:epidemiology* / Q000209:etiology; D012296:Rift Valley fever virus / Q000276:immunology; D016036:Seroepidemiologic Studies; D013636:Tanzania / Q000453:epidemiology
D000914:Antibodies, Viral