Phenotypic and genotypic characterization of biofilm formation in Staphylococcus haemolyticus.
Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to produce biofilm has contributed to its emergence as a nosocomial pathogen. In this study, some growth conditions were tested to determine their influence on biofilm formation. Brain-heart infusion (BHI) broth containing glucose was used to screen 64 clinical strains. A strong biofilm producer strain showed cells surrounded by a thick layer of extracellular matrix. The presence of atlE, fbp, bap, and icaA genes was analyzed. We concluded that S. haemolyticus biofilm production can be increased with cells grown in BHI, and highlighted that it could be an ica-independent process.
Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
Elaine M
EM
Barros
Moara
M
Lemos
Thais
T
Souto-Padrón
Marcia
M
Giambiagi-deMarval
D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D001704:Biopolymers / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D010641:Phenotype; D013203:Staphylococcal Infections / Q000382:microbiology; D041162:Staphylococcus haemolyticus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
D001704:Biopolymers; D003470:Culture Media