Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35.
PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer's disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Mingmin
M
Yan
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Shanping
S
Mao
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Huimin
H
Dong
Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Baohui
B
Liu
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Qian
Q
Zhang
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Gaofeng
G
Pan
Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Zhiping
Z
Fu
PC12 cells; Schisandrin B; amyloid precursor protein; amyloid β-protein 25–35; neural protection; vacuolar protein sorting 35