Relationship between male sterility and β-1,3-glucanase activity and callose deposition-related gene expression in wheat (Triticum aestivum L.).
In previous studies, we first isolated one different protein β-1,3-glucanase using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from normal wheat (Triticum aestivum L.) and chemical hybridization agent-induced male sterility (CIMS) wheat. In this experiment, β-1,3-glucanase activity and the expression of a callose deposition-related gene, UDP-glucose phosphorylase (UGPase), were determinate in normal, CIMS, and genetic male sterility (GS) wheat. β-1,3-glucanase activity was significantly different between the fertile and sterile lines during callose synthesis and degradation, but there was no difference between CIMS and GS wheat. The UGPase gene of callose deposition was highly expressed in the meiophase and sharply decreased in the tetrad stage. However, the expression of the UGPase gene was significantly different between the fertile and sterile lines. These data indicated that β-1,3-glucanase activity and the expression of the UGPase gene play important roles in the male sterility of wheat. Consequently, pollen mother cells (PMCs) might degenerate at the early meiosis stage, and differences in UGPase gene expression and β-1,3-glucanase activity might eventually result in complete pollen collapse. In addition, the critical period of anther abortion might be the meiosis stage to the tetrad stage rather than what we previously thought, the mononuclear period.
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
H Z
HZ
Liu
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China zhanggsh58@aliyun.com.
G S
GS
Zhang
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
W W
WW
Zhu
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
Q S
QS
Ba
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
N
N
Niu
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
J W
JW
Wang
Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, China.
S C
SC
Ma
Department of Life Sciences, Zhoukou Normal University, Zhoukou, Henan, China.
J S
JS
Wang
D018076:DNA, Complementary / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins; D011058:Pollen / Q000378:metabolism / Q000648:ultrastructure; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014908:Triticum / Q000201:enzymology* / Q000235:genetics* / Q000648:ultrastructure
D018076:DNA, Complementary; D005936:Glucans; D010940:Plant Proteins; D012337:RNA, Ribosomal, 18S; C048306:callose; D009713:Nucleotidyltransferases; D043326:Glucan 1,3-beta-Glucosidase