A metastatic hepatoma model of rats using the 13762-MAT-B-III cell line: basic characteristics and potential as a tool for interventional oncology experiments.
OBJECTIVE
To evaluate the characteristics of a 13762-MAT-B-III model in the rat liver and to assess the adequacy of the model for transarterial embolization (TAE) study.


METHODS
One hundred thousand 13762-MAT-B-III rat breast cancer cells were inoculated into the livers of 11 Fisher 344 rats. Natural course via magnetic resonance imaging (MRI) follow-up, histological characteristics and tumor response after TAE were evaluated.


RESULTS
The tumor induction rate of the 13762-MAT-B-III hepatoma model was 100%. Except for one tumor that started to regress after 14 days, the 13762-MAT-B-III hepatomas showed rapid growth, up to 13.1±1.4 mm, at 21 days. Peritoneal seeding was observed in all rats. TAE was conducted successfully in three rats on day 11. The TAE-treated hepatomas were significantly smaller on day 21 (p=0.040) and had a significantly greater apoptosis ratio (p=0.046).


CONCLUSIONS
The 13762-MAT-B-III hepatoma model can be useful in many interventional oncology studies by providing consistent and rapidly growing tumors.
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Jin Woo
JW
Choi
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea angiointervention@gmail.com.
Hyo-Cheol
HC
Kim
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Song Yi
SY
Baek
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Young Jin
YJ
Ryu
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Jin Wook
JW
Chung
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000556:secondary / Q000628:therapy*; D045744:Cell Line, Tumor; D016461:Chemoembolization, Therapeutic; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D008297:Male; D011916:Rats, Inbred F344
13762-MAT-B-III; animal model; liver; metastasis; transarterial embolization