The DREAM complex promotes gene body H2A.Z for target repression.
The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual pattern of high gene body HTZ-1/H2A.Z. In mutants of lin-35, the sole p130/Rb-like gene in C. elegans, DREAM targets have reduced gene body HTZ-1/H2A.Z and increased expression. Consistent with a repressive role for gene body H2A.Z, many DREAM targets are up-regulated in htz-1/H2A.Z mutants. Our results indicate that the DREAM complex facilitates high gene body HTZ-1/H2A.Z, which plays a role in target gene repression.
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom;
Isabel
I
Latorre
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom;
Michael A
MA
Chesney
Molecular, Cell, and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA;
Jacob M
JM
Garrigues
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom;
Przemyslaw
P
Stempor
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom;
Alex
A
Appert
EMBL-CRG Systems Biology Unit, Centre for Genomic Regulation (CRG), Universitat Pompeu Fabra (UPF), Barcelona 08003, Spain.
Mirko
M
Francesconi
Molecular, Cell, and Developmental Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA;
Susan
S
Strome
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom; j.ahringer@gurdon.cam.ac.uk.
Julie
J
Ahringer
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018816:Genes, cdc / Q000235:genetics; D006657:Histones / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D059467:Transcriptome
D029742:Caenorhabditis elegans Proteins; D006657:Histones
C. elegans; H2A.Z; Retinoblastoma/DREAM; transcriptional repression