Affinity chromatography: a historical perspective.
Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation of new binding agents, supports, and immobilization methods for this method are considered. Various applications of affinity chromatography are also summarized, as well as the influence this field has played in the creation of other affinity-based separation or analysis methods.
Department of Chemistry, University of Nebraska-Lincoln, 704 Hamilton Hall, 639 N 12 Street, Lincoln, NE, 68588-0304, USA, dhage1@unl.edu.
David S
DS
Hage
Ryan
R
Matsuda
D002846:Chromatography, Affinity / Q000266:history* / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D008024:Ligands
D008024:Ligands