Syntenin is involved in the bacteria clearance response of kuruma shrimp (Marsupenaeus japonicus).
Syntenin is a multifunctional cytosolic adaptor protein that contributes to cell migration, proliferation, attachment, and apoptosis, as well as immune response to virus, in vertebrates. However, the functions of syntenin in the antibacterial response of invertebrates remain unclear. In this study, we identified a syntenin-like gene (MjSyn) from the kuruma shrimp (Marsupenaeus japonicus) and detected its function in the antibacterial immunity of shrimp. The full-length MjSyn was 1223 bp with a 963 bp open reading frame that encodes 320 amino acids. The deduced MjSyn proteins contained two atypical PDZ domains (sequence repeat that was first reported in the postsynaptic density protein or PSD-95, DlgA, and ZO-1 protein), an N-terminal domain, and a C-terminal domain. Reverse transcription (RT)-PCR results showed that MjSyn was expressed in all tested tissues. Quantitative real-time PCR analysis revealed that MjSyn transcripts in the hemocyte, gill, and intestine were signiﬁcantly induced at various time points after infection with Staphylococcus aureus and Vibrio anguillarum. The knockdown of the expression of MjSyn by RNA interference resulted in a significant decrease in the phagocytic ability and increased bacteria number in vivo of shrimp. Moreover, the expression of MjCnx, a cytoplasma and membrane location lectin chaperone protein, was inhibited in the MjSyn-knocked down shrimp, which indicated a possible calnexin-related way. Thus, the MjSyn participates in the bacterial clearance response of kuruma shrimp, thereby providing new insight into the function of this kind of important adaptor protein.
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.
Qian
Q
Liu
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.
Xiao-wei
XW
Chen
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.
Chun-jing
CJ
Che
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China.
Ding
D
Ding
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong, 250100, China. Electronic address: cjkang@sdu.edu.cn.
Cui-jie
CJ
Kang
D000818:Animals; D001419:Bacteria / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology*; D005880:Gills / Q000378:metabolism*; D006434:Hemocytes / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D053676:Syntenins / Q000235:genetics* / Q000276:immunology
D017931:DNA Primers; D018076:DNA, Complementary; D053676:Syntenins
Bacteria clearance; Innate immunity; Phagocytosis; Shrimp; Syntenin