Transmembrane protein 173 inhibits RANKL-induced osteoclast differentiation.
Tmem173 was identified as a growth inhibitor associated with major histocompatibility complex (MHC) class II and a potential stimulator for IFN-β, an innate immune inducer and a negative feedback controller for RANKL-induced osteoclast differentiation of monocytic macrophage cells. In this study, we confirmed that transmembrane protein 173 (Tmem173) overexpression inhibited the expression of osteoclast-specific genes, tartrate-resistant acid phosphatase (TRAP), cathepsin K, and matrix metalloproteinase-9 (MMP-9), as well as bone resorption pit formation in RANKL-treated RAW 264.7 cells. Activation of osteoclast-specific transcription factors, c-Fos and nuclear factor of activated T cells cytoplasmic-1 (NFATc1), and RANKL-induced activation of ERK were also down-regulated by Tmem173 overexpression. Collectively, these results suggest that Tmem173 plays a regulatory role in RANKL-RANK-mediated signaling in osteoclastogenesis.
Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea; Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Chung-Hyeon
CH
Choe
Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea.
In Sun
IS
Park
Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Jisang
J
Park
Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea.
Kang-Yeol
KY
Yu
Department of Oral and Maxillofacial Surgery, Korea University Ansan Hospital, Ansan 425-707, Republic of Korea.
Hyonseok
H
Jang
Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea. Electronic address: ju226@jbmi.re.kr.
Ju
J
Kim
Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: yongsuk@jbnu.ac.kr.
Yong-Suk
YS
Jang
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000099197:STING Protein
D008565:Membrane Proteins; D053244:Osteoprotegerin; D053245:RANK Ligand; C530855:Sting1 protein, mouse; D000099197:STING Protein; C505689:Tnfsf11 protein, mouse
Osteoclastogenesis; RANK ligand; Tartrate-resistant acid phosphatase; Transmembrane protein 173