Mouse models of bone healing: fracture, marrow ablation, and distraction osteogenesis.
Three commonly used murine surgical models of bone healing [closed fracture with intramedullary fixation, distraction osteogenesis (DO), and marrow ablation by reaming] are presented. Detailed surgical protocols for each model are outlined. The nature of the regenerative processes and the types of research questions that may be addressed with these models are briefly outlined. The relative strengths and weaknesses of these models are compared to a number of other surgical models that are used to address similar research questions.
Orthopaedic Research Laboratory, Boston University School of Medicine, Boston, Massachusetts.|Department of Orthopaedic Surgery, Boston Medical Center, Boston, Massachusetts.
Kyle
K
Lybrand
Orthopaedic Research Laboratory, Boston University School of Medicine, Boston, Massachusetts.
Beth
B
Bragdon
Orthopaedic Research Laboratory, Boston University School of Medicine, Boston, Massachusetts.
Louis
L
Gerstenfeld
D055011:Ablation Techniques; D000818:Animals; D001853:Bone Marrow / Q000601:surgery*; D005594:Fracture Fixation, Intramedullary; D050723:Fractures, Bone / Q000601:surgery*; D005596:Fractures, Closed / Q000601:surgery; D006801:Humans; D051379:Mice; D023421:Models, Animal; D019857:Osteogenesis, Distraction
distraction osteogenesis; fracture; murine models; orthopedic surgery; reaming