Real-time ultrasound-guided needle injection of the mouse jugular vein.
This unit describes a novel method for direct venous injection into mice that offers potentially significant advantages over commonly used mouse vein injection techniques. This is achieved via percutaneous needle placement into the mouse jugular vein under real-time B-mode ultrasound (US) imaging. Real-time US imaging of the injection process allows for immediate determination of the overall success of injection. Unique, and potentially significant, advantages of this technique over others include: (1) direct visual confirmation of needle tip placement in the lumen of the vein, (2) immediate visual detection of extravascular extravasation of injectate, when compared to blinded techniques, such as tail vein injections, and (3) reduced morbidity and mortality compared to surgical vascular access techniques (i.e., jugular vein cannulation). This technique may lead to more accurate determination of the success of the injection procedure for each mouse, thus improving the quality of acquired data in dependent mouse experiments.
University of South Florida, Division of Comparative Medicine, Tampa, Florida.
Dominique
D
Abrahams
Department of Cancer Imaging and Metabolism, Moffitt Cancer Center and Research Institute, Tampa, Florida.
Yolaine
Y
Jeune-Smith
University of South Florida, Division of Comparative Medicine, Tampa, Florida.
Aimee
A
Bode
University of South Florida, Division of Comparative Medicine, Tampa, Florida.
Margaret
M
Baldwin
Department of Cancer Imaging and Metabolism and Department of Diagnostic Imaging, Moffitt Cancer Center and Research Institute, Tampa, Florida.
Jung W
JW
Choi
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007275:Injections, Intravenous / Q000379:methods*; D007601:Jugular Veins / Q000000981:diagnostic imaging*; D051379:Mice; D018084:Ultrasonography, Interventional / Q000295:instrumentation*
jugular vein injection; mouse jugular vein; mouse vein injection; tail vein injection; ultrasound guided injection