[Study on material basis of essential oil from Yin Teng Gu Bi Kang prescription on activating blood circulation].
OBJECTIVE
To explore the component difference of the serum containing essential oil from Yin Teng Gu Bi Kang prescription in pathologic and physiologic rat models, and to reveal the material basis of its efficacy of activating blood circulation.


METHODS
The essential oils were obtained by CO2 supercritical fluid extraction and the ingredients of the essential oils in vitro and in vivo (under physiological and pathological status) were analyzed by GC-MS to compare differences of the essential oil under physiological and pathological status in rats.


RESULTS
32 components were identified with the main components of Z-ligustilide (39.23%) and d-limonene (21.7%) in the essential oil. In vivo analysis on the essential oil indicated that 16 components were identified, 7 existed originally in essential oil and 9 were metabolites under physiological status; while 22 components were identified, 10 existed originally in essential oil and 12 were metabolites under pathological status (acute blood stasis). There were 7 common prototypes and 8 common metabolites under different physiological status.


CONCLUSIONS
The absorption and metabolism of essential oils were affected by blood stasis and the compounds migrating to blood may be the effective substance in activating blood circulation.
Yuan-Qing
YQ
Wang
Jian-Ye
JY
Yan
Li-Min
LM
Gong
Kun
K
Luo
Shun-Xiang
SX
Li
Yan-Tao
YT
Yang
Yu
Y
Xie
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D000818:Animals; D001775:Blood Circulation / Q000187:drug effects*; D025924:Chromatography, Supercritical Fluid; D053138:Cyclohexenes; D008401:Gas Chromatography-Mass Spectrometry; D000077222:Limonene; D009822:Oils, Volatile / Q000494:pharmacology*; D051381:Rats; D013729:Terpenes
D053138:Cyclohexenes; D009822:Oils, Volatile; D013729:Terpenes; C027820:ligustilide; D000077222:Limonene; D015107:4-Butyrolactone