A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance.
The mycotoxin deoxynivalenol (DON) acts as a disease virulence factor for Fusarium fungi, and tolerance of DON enhances wheat resistance to Fusarium head blight (FHB) disease. Two variants of an ATP-binding cassette (ABC) family C transporter gene were cloned from DON-treated wheat mRNA, namely TaABCC3.1 and TaABCC3.2. These represent two of three putative genes identified on chromosomes 3A, 3B, and 3D of the wheat genome sequence. Variant TaABCC3.1 represents the DON-responsive transcript previously associated with DON resistance in wheat. PCR-based mapping and in silico sequence analyses located TaABCC3.1 to the short arm of wheat chromosome 3B (not within the FHB resistance quantitative trait locus Fhb1). In silico analyses of microarray data indicated that TaABCC3 genes are expressed in reproductive tissue and roots, and in response to the DON producer Fusarium graminearum. Gene expression studies showed that TaABCC3.1 is activated as part of the early host response to DON and in response to the FHB defence hormone jasmonic acid. Virus-induced gene silencing (VIGS) confirmed that TaABCC3 genes contributed to DON tolerance. VIGS was performed using two independent viral construct applications: one specifically targeted TaABCC3.1 for silencing, while the other targeted this gene and the chromosome 3A homeologue. In both instances, VIGS resulted in more toxin-induced discoloration of spikelets, compared with the DON effects in non-silenced spikelets at 14 d after toxin treatment (≥2.2-fold increase, P<0.05). Silencing by both VIGS constructs enhanced head ripening, and especially so in DON-treated heads. VIGS of TaABCC3 genes also reduced the grain number by more than 28% (P<0.05), both with and without DON treatment, and the effects were greater for the construct that targeted the two homeologues. Hence, DON-responsive TaABCC3 genes warrant further study to determine their potential as disease resistance breeding targets and their function in grain formation and ripening.
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland.
Stephanie
S
Walter
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland.
Amal
A
Kahla
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland.
Chanemoughasoundharam
C
Arunachalam
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland.
Alexandre
A
Perochon
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland.
Mojibur R
MR
Khan
USDA-ARS, Crop Production and Pest Control Research Unit and Purdue University, Department of Agronomy, 915 West Street, West Lafayette, IN 47907-2054, USA.
Steven R
SR
Scofield
UCD Earth Institute and School of Biology & Environment Science, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland fiona.doohan@ucd.ie.
Fiona M
FM
Doohan
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005670:Fusarium / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D009183:Mycotoxins / Q000378:metabolism / Q000494:pharmacology*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological; D014255:Trichothecenes / Q000378:metabolism / Q000494:pharmacology*; D014908:Triticum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D037521:Virulence Factors
D018528:ATP-Binding Cassette Transporters; D009183:Mycotoxins; D010940:Plant Proteins; D014255:Trichothecenes; D037521:Virulence Factors; C007262:deoxynivalenol
ABC transporter; Fusarium head blight; breeding; deoxynivalenol; grain formation; multidrug resistance; ripening; toxin resistance; wheat.