T-style keratoprosthesis based on surface-modified poly (2-hydroxyethyl methacrylate) hydrogel for cornea repairs.
Corneal disease is a common cause of blindness, and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents a novel T-style design of a keratoprosthesis and its preparation methods, in which a mechanically and structurally effective artificial cornea is made based on a poly(2-hydroxyethyl methacrylate) hydrogel. The porous skirt was modified with hyaluronic acid and cationized gelatin, and the bottom of the optical column was coated with poly(ethylene glycol). The physical properties of the T-style Kpro were analyzed using ultraviolet and visible spectrophotometry and electron scanning microscopy. The surface chemical properties were characterized using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The surface modification in the spongy skirt promoted cell adhesion and produced a firm bond between the corneal tissue and the implant device, while the surface modification in the optic column resisted cell adhesion and prevented retroprosthetic membrane formation. Through improved surgical techniques, the novel T-style keratoprosthesis provides enough mechanical stability to facilitate long-term biointegration with the host environment. In vivo implantation experiments showed that the T-style keratoprosthesis is a promising cornea alternative for patients with severe limbal stem cell deficiency and corneal opacity.
Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China.
Jun
J
Xiang
Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China; State Key Laboratory of Molecular Engineering of Polymers, Fudan University, China.
Jianguo
J
Sun
Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China.
Jiaxu
J
Hong
Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China.
Wentao
W
Wang
Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China.
Anji
A
Wei
Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China.
Qihua
Q
Le
Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, China. Electronic address: jianjiang-xu@163.com.
Jianjiang
J
Xu
D000818:Animals; D001187:Artificial Organs; D002056:Burns / Q000473:pathology / Q000628:therapy; D002448:Cell Adhesion; D003315:Cornea / Q000473:pathology*; D003319:Corneal Stroma / Q000473:pathology; D004195:Disease Models, Animal; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D019736:Prostheses and Implants; D011474:Prosthesis Design; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D034421:Sus scrofa; D014867:Water; D014945:Wound Healing
D014867:Water; D011102:Polyhydroxyethyl Methacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
Artificial cornea; Biointegration; Cell adhesion; PHEMA hydrogel; Surface modification