Two genes conferring resistance to Pythium stalk rot in maize inbred line Qi319.
Stalk rots are destructive diseases in maize around the world, and are most often caused by the pathogen Pythium, Fusarium and other fungi. The most efficient management for controlling stalk rots is to breed resistant cultivars. Pythium stalk rot can cause serious yield loss on maize, and to find the resistance genes from the existing germplasm is the basis to develop Pythium-resistance hybrid lines. In this study, we investigated the genetic resistance to Pythium stalk rot in inbred line Qi319 using F2 and F2:3 population, and found that the resistance to Pythium inflatum in Qi319 was conferred by two independently inherited dominant genes, RpiQI319-1 and RpiQI319-2. Linkage analysis uncovered that the RpiQI319-1 co-segregated with markers bnlg1203, and bnlg2057 on chromosome 1, and that the RpiQI319-2 locus co-segregated with markers umc2069 and bnlg1716 on chromosome 10. The RpiQI319-1 locus was further mapped into a ~500-kb interval flanked by markers SSRZ33 and SSRZ47. These results will facilitate marker-assisted selection of Pythium stalk rot-resistant cultivars in maize breeding. To our knowledge, this is the first report on the resistance to P. inflatum in the inbred line Qi319, and is also the first description of two independently inherited dominant genes conferring the resistance of Pythium stalk rot in maize.
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Feng-Jing
FJ
Song
Ming-Gang
MG
Xiao
Can-Xing
CX
Duan
Hong-Jie
HJ
Li
Zhen-Dong
ZD
Zhu
Bao-Tao
BT
Liu
Su-Li
SL
Sun
Xiao-Fei
XF
Wu
Xiao-Ming
XM
Wang
D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D054884:Host-Pathogen Interactions; D007178:Inbreeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011775:Pythium / Q000502:physiology; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology
D018744:DNA, Plant; D005819:Genetic Markers