Second derivative multispectral algorithm for quantitative assessment of cutaneous tissue oxygenation.
We report a second derivative multispectral algorithm for quantitative assessment of cutaneous tissue oxygen saturation (StO₂). The algorithm is based on a forward model of light transport in multilayered skin tissue and an inverse algorithm for StO₂ reconstruction. Based on the forward simulation results, a parameter of a second derivative ratio (SDR) is derived as a function of cutaneous tissue StO₂. The SDR function is optimized at a wavelength set of 544, 552, 568, 576, 592, and 600 nm so that cutaneous tissue StO₂ can be derived with minimal artifacts by blood concentration, tissue scattering, and melanin concentration. The proposed multispectral StO₂ imaging algorithm is verified in both benchtop and in vivo experiments. The experimental results show that the proposed multispectral imaging algorithm is able to map cutaneous tissue StO₂ in high temporal resolution with reduced measurement artifacts induced by different skin conditions in comparison with other three commercial tissue oxygen measurement systems. These results indicate that the multispectral StO₂ imaging technique has the potential for noninvasive and quantitative assessment of skin tissue oxygenation with a high temporal resolution.
The Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210, United States.
Jiwei
J
Huang
University of Science and Technology of China, Department of Precision Machinery and Precision Instrumentation, Hefei, Anhui 230027, China.
Shiwu
S
Zhang
The Ohio State University, Department of Surgery, Columbus, Ohio 43210, United States.
Surya
S
Gnyawali
The Ohio State University, Department of Surgery, Columbus, Ohio 43210, United States.
Chandan K
CK
Sen
The Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210, United StatesbUniversity of Science and Technology of China, Department of Precision Machinery and Precision Instrumentation, Hefei, Anhui 230027, China.
Ronald X
RX
Xu
D000465:Algorithms; D000704:Analysis of Variance; D006801:Humans; D008543:Melanins; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D012867:Skin / Q000098:blood supply / Q000378:metabolism*; D012880:Skin Pigmentation
D008543:Melanins; D010100:Oxygen