Comparison of extraction methods of total microbial DNA from freshwater.
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water. DNA extraction was analyzed by agarose gel electrophoresis, ultraviolet-spectroscopy, restriction enzyme digestion, and polymerase chain reaction. The results showed that the modified filtration-membrane method was the most efficient for extracting microbial DNA from freshwater with high integrity and purity and is suitable for molecular applications.
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
P
P
Li
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
S F
SF
Yang
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
B B
BB
Lv
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
K
K
Zhao
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
M F
MF
Lin
Institute of ECO-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.
S
S
Zhou
Institute of ECO-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.
X
X
Song
Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China sunsite@126.com.
X M
XM
Tang
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002498:Centrifugation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology
D004269:DNA, Bacterial