Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate isolated from the leaves of Andrographis lineata.Wall. ex. Nees suppress adipogenesis in 3T3-L1 preadipocytes for type 2 diabetes.
The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of Andrographis lineata (EtALL) which suppress the differentiation of 3T3-L1 adipocytes. The ethanolic leaf extract was subjected to bioassay guided fractionation in 3T3-L1 cell lines. Five fractions were isolated from the EtALL extract by column chromatography. All the Fractions (I-V) along with EtALL were screened for adipogenesis activity (Oil-Red-O staining).The fraction which showed maximum adipogenesis activity was purified by thin layer chromatography. The bioactive Fraction IV was found to have maximum adipogenic (96.83%) activity and the activity was comparable to Rosiglitazone. The spectroscopic data analysis reveals that, the isolated bioactive compound was Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate (DTδT), a combination of truxillic and truxinic acid derivative. DTδT showed insulin mimicking (131.2%), sensitizing (810.02%) and adipogenic activity (80.23%). Hence our present study concluded that, Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate isolated from the ethanolic leaf extract of Andrographis lineata stimulates glucose uptake, potentiates insulin-stimulated glucose in 3T3-L1 adipocytes without increasing adiposity.
Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamilnadu, India.
Vijayakumar Sudarshana
VS
Deepa
Department of Biotechnology, Anna University Chennai (BIT Campus), Tiruchirappalli, Tamilnadu, India.
Krishnasamy
K
Rajaram
Department of Biotechnology, Anna University Chennai (BIT Campus), Tiruchirappalli, Tamilnadu, India.
Periyasamy
P
Sureshkumar
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003503:Cyclobutanes / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry
D003503:Cyclobutanes; D007004:Hypoglycemic Agents; D007328:Insulin; C000597069:dimethyl 3, 3', 4, 4'-tetrahydroxy-delta-truxinate