Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Ming
M
Yuan
Cambridge Suda Genome Research Center, Soochow University, Soochow, Jiangsu Province, People's Republic of China Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Wensheng
W
Zhang
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Jun
J
Wang
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Chadwan
C
Al Yaghchi
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Jahangir
J
Ahmed
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
Louisa
L
Chard
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom Sino-British Research Centre for Molecular Oncology, Zhengzhou University, China bci-director@qmul.ac.uk Yaohe.wang@qmul.ac.uk.
Nick R
NR
Lemoine
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom Sino-British Research Centre for Molecular Oncology, Zhengzhou University, China bci-director@qmul.ac.uk Yaohe.wang@qmul.ac.uk.
Yaohe
Y
Wang
D064113:CRISPR-Cas Systems; D016679:Genome, Viral; D008967:Molecular Biology / Q000379:methods*; D011995:Recombination, Genetic; D013678:Technology, Pharmaceutical / Q000379:methods; D014616:Vaccinia virus / Q000235:genetics*