Protective effect of Lactobacillus plantarum 21, a probiotic on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats.
Inflammatory mediators play a crucial role in ulcerative colitis (UC). The present study was aimed to evaluate the effects of Lactobacillus plantarum 21 (LAB 21) on inflammatory mediators in trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats. The inflammatory response was assessed by changes in colon morphology, histopathology, and measurement of reduced glutathione (GSH), lipid peroxidation (TBARS), nitric oxide (NO), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin 10 (IL-10) mRNA and protein levels by ELISA. Besides, protein expressions of IL-1β and IL-10 were also evaluated by western blot. Treatment with LAB 21 (1×10(10)CFU/rat/day) and sulfasalazine (500mgkg(-1) body weight) for 14days after induction of colitis, significantly decreased TBARS, NO and increased GSH concentration. The protein and mRNA expressions of IL-1β and TNFα were down-regulated, whereas, protein and mRNA expression of IL-10 was up-regulated in LAB 21-treated rats. Moreover, LAB 21 attenuated the macroscopic colonic damage, histopathological changes induced by TNBS. These results suggest that LAB 21 may be effective in the treatment of UC by immunomodulatory and antioxidant properties.
Department of Pharmacology & Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: satish513512@gmail.com.
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Satish Kumar
Livestock Products & Technology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India.
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Kondal Reddy
Department of Pharmacology & Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India.
A Gopala
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Reddy
Department of Molecular Genetics and Breeding, Directorate of Poultry Research, Rajendranagar, Hyderabad 500 030, India.
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Vinoth
Department of Veterinary Pathology, NTR College of Veterinary Science, Gannavaram 521102, India.
Sudha Rani Chowdary
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Department of Pharmacology & Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India.
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Boobalan
Department of Pharmacology & Toxicology, NTR College of Veterinary Science, Gannavaram 521102, India.
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Rao
D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000473:pathology; D005260:Female; D005978:Glutathione / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D048191:Lactiplantibacillus plantarum; D009569:Nitric Oxide / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D053583:Interleukin-1beta; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; D014302:Trinitrobenzenesulfonic Acid; D005978:Glutathione
Colitis; Cytokines; Free radicals; LAB 21; NO