Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds from traditional Chinese medicine and natural products.
Screening and identifying active compounds from traditional Chinese medicine (TCM) and other natural products plays an important role in drug discovery. Here, we describe a magnetic beads-based multi-target affinity selection-mass spectrometry approach for screening bioactive compounds from natural products. Key steps and parameters including activation of magnetic beads, enzyme/protein immobilization, characterization of functional magnetic beads, screening and identifying active compounds from a complex mixture by LC/MS, are illustrated. The proposed approach is rapid and efficient in screening and identification of bioactive compounds from complex natural products.
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, 866 Yuhangtang Rd, Hangzhou, 310058, China.
Yaqi
Y
Chen
Zhui
Z
Chen
Yi
Y
Wang
D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D059346:Magnets / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008516:Medicine, Chinese Traditional; D008863:Microspheres; D015203:Reproducibility of Results
D001688:Biological Products; D004800:Enzymes, Immobilized