Comparison of different sampling types across the rearing period in broiler flocks for isolation of Campylobacter spp.
Campylobacter is the most common bacterial cause of human gastrointestinal disease in most developed countries. It is generally accepted that poultry products are a significant source of foodborne Campylobacter infections in humans. Assessing the effectiveness of any potential intervention at farm level requires monitoring of the Campylobacter status of broiler flocks, using appropriate sampling methods. The aim of this study was to assess the influence of the sample type across the rearing period for the detection of Campylobacter spp. at farm level. During this study, 21 commercial broiler farms were intensively sampled. Each farm was visited and sampled at different times during the rearing period (d 1, 7, 14, 21, 28, 35, and 42). On the first day of rearing, the status of the house and the day-old flock was evaluated, and environmental and cecal samples were collected. During rearing, 4 different sample types were collected: feces with sock swabs (sock swabs), feces directly from the litter (feces), cloacal swabs, and cecal content. All samples were analyzed according to ISO 10272-1:2006 (Annex E) and also by direct culture. The results of this study showed that Campylobacter spp. were detected in all of the sample types on d 14 of rearing. From this point on, the detection increased significantly, with a maximum detection rate by the end of rearing, regardless of the sample type. All samples that were negative upon direct culture were also negative after pre-enrichment. At the end of rearing, the percentage of samples positive for Campylobacter spp. was 71.4% for cecal samples, 61.9% for cloacal swabs, 45.2% for sock swabs, and 69.1% for fecal samples. C. jejuni was detected in all the sample types, with positive rates ranging from 67.1 to 76.0% for cecal samples and cloacal content, respectively. Cecal samples, cloacal swabs, and fecal samples cultured by direct plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA) without pre-enrichment have the same sensitivity for detection of Campylobacter spp. in broiler flocks independent of the day of rearing.
Biomedical Sciences Institute, Department of Animal Production, Animal Health, Veterinary Public Health and Food Technology, Veterinary Faculty, University CEU-Cardenal Herrera, C/ Tirant Lo Blanc 7, 46115 Alfara del Patriarca, Valencia, Spain sofia.ingresa@uch.ceu.es.
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Ingresa-Capaccioni
Biomedical Sciences Institute, Department of Animal Production, Animal Health, Veterinary Public Health and Food Technology, Veterinary Faculty, University CEU-Cardenal Herrera, C/ Tirant Lo Blanc 7, 46115 Alfara del Patriarca, Valencia, Spain.
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González-Bodí
Institute of Science and Animal Technology, Polytechnic University of Valencia, C/ Camino de Vera s/n, 46022, Valencia, Spain.
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Jiménez-Trigos
Institute of Science and Animal Technology, Polytechnic University of Valencia, C/ Camino de Vera s/n, 46022, Valencia, Spain.
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Marco-Jiménez
Centre of Poultry Quality and Feed of the Valencian Region (CECAV), C/ Nules 16, 12539 Alquerías del Niño Perdido, Castellón, Spain.
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Catalá
Biomedical Sciences Institute, Department of Animal Production, Animal Health, Veterinary Public Health and Food Technology, Veterinary Faculty, University CEU-Cardenal Herrera, C/ Tirant Lo Blanc 7, 46115 Alfara del Patriarca, Valencia, Spain.
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Vega
Biomedical Sciences Institute, Department of Animal Production, Animal Health, Veterinary Public Health and Food Technology, Veterinary Faculty, University CEU-Cardenal Herrera, C/ Tirant Lo Blanc 7, 46115 Alfara del Patriarca, Valencia, Spain.
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Marin
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary*; D002167:Campylobacter / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D002988:Cloaca / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005766:Gastrointestinal Contents / Q000382:microbiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology
Campylobacter; broiler; sample type