Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags.
Parenteral nutrition bags for newborns were found contaminated by a previously undescribed member of the family Enterobacteriaceae. The six isolates studied by rrs gene (encoding 16S rRNA) sequence analysis and multi-locus sequence analysis (MLSA) formed a discrete branch close to the genera Ewingella, Rahnella, Yersinia,Hafnia and Serratia. Phenotypically, the new taxon was distinct from these five genera. The new taxon gave positive results in Voges-Proskauer, Simmons citrate and o-nitrophenyl-β-galactoside hydrolysis tests; fermented d-glucose, d-mannitol, l-rhamnose, melibiose, l-arabinose and d-xylose; hydrolysed aesculin; and did not ferment maltose, trehalose, raffinose, d-sorbitol, sucrose or cellobiose. Tests for motility, gas production, urease, gelatinase and nitrate reduction were also negative. All isolates failed to grow at 37 °C. The DNA G+C content of strain 130333T was 53 mol%. On the basis of data obtained in this study, the six isolates represent a novel species of a new genus in the family Enterobacteriaceae, named Rouxiella chamberiensis gen. nov., sp. nov. The type strain of the type species is 130333T ( = CIP 110714T = DSM 28324T).
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Anne
A
Le Flèche-Matéos
Laboratoire de bactériologie, Centre Hospitalier de Chambéry, F-73011 Chambéry Cedex, France.
Marion
M
Levast
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Fabienne
F
Lomprez
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Yolande
Y
Arnoux
Laboratoire de bactériologie, Centre Hospitalier de Chambéry, F-73011 Chambéry Cedex, France.
Clément
C
Andonian
Laboratoire de Biologie-Sécurité-Environnement, Centre Hospitalo-Universitaire de Lyon, F-69437 Lyon Cedex 03, France.
Michel
M
Perraud
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Véronique
V
Vincent
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Meriadeg
M
Ar Gouilh
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Jean-Michel
JM
Thiberge
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Mathias
M
Vandenbogaert
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Laure
L
Diancourt
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Valérie
V
Caro
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Ana Maria
AM
Burguière
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Jean-Claude
JC
Manuguerra
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004866:Equipment Contamination; D005602:France; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010288:Parenteral Nutrition; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D002241:Carbohydrates; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S