Biomimetic magnetic silk scaffolds.
Magnetic silk fibroin protein (SFP) scaffolds integrating magnetic materials and featuring magnetic gradients were prepared for potential utility in magnetic-field assisted tissue engineering. Magnetic nanoparticles (MNPs) were introduced into SFP scaffolds via dip-coating methods, resulting in magnetic SFP scaffolds with different strengths of magnetization. Magnetic SFP scaffolds showed excellent hyperthermia properties achieving temperature increases up to 8 °C in about 100 s. The scaffolds were not toxic to osteogenic cells and improved cell adhesion and proliferation. These findings suggest that tailored magnetized silk-based biomaterials can be engineered with interesting features for biomaterials and tissue-engineering applications.
†Consiglio Nazionale delle Ricerche-Institute for Nanostructured Materials, I-40129 Bologna-Roma, Italy.|‡Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, United States.|§Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Sangram K
SK
Samal
Mamoni
M
Dash
⊥Laboratory of Biomechanics and Technology Innovation, NABI, Rizzoli Orthopaedic Institute, 40136 Bologna, Italy.
Tatiana
T
Shelyakova
#Department of Basic Medical Science - Tissue Engineering Group, Ghent University, De Pintelaan 185 (6B3), 9000 Ghent, Belgium.
Heidi A
HA
Declercq
∇Dresden High Magnetic Field Laboratory (HLD-EMFL), Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328 Dresden, Germany.
Marc
M
Uhlarz
○International Iberian Nanotechnology Laboratory (INL), Av. Mestre José Veiga, 4715-330 Braga, Portugal.
Manuel
M
Bañobre-López
Peter
P
Dubruel
#Department of Basic Medical Science - Tissue Engineering Group, Ghent University, De Pintelaan 185 (6B3), 9000 Ghent, Belgium.
Maria
M
Cornelissen
∇Dresden High Magnetic Field Laboratory (HLD-EMFL), Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328 Dresden, Germany.
Thomas
T
Herrmannsdörfer
○International Iberian Nanotechnology Laboratory (INL), Av. Mestre José Veiga, 4715-330 Braga, Portugal.
Jose
J
Rivas
†Consiglio Nazionale delle Ricerche-Institute for Nanostructured Materials, I-40129 Bologna-Roma, Italy.
Giuseppina
G
Padeletti
§Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Stefaan
S
De Smedt
§Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Kevin
K
Braeckmans
‡Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, United States.
David L
DL
Kaplan
†Consiglio Nazionale delle Ricerche-Institute for Nanostructured Materials, I-40129 Bologna-Roma, Italy.
V Alek
VA
Dediu
D016475:3T3 Cells; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005349:Fibroins / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D051379:Mice; D010316:Particle Size; D054457:Tissue Scaffolds
D058185:Magnetite Nanoparticles; D005349:Fibroins
biomaterials; hyperthermia; magnetic field; magnetic gradient; magnetic nanoparticles; magnetic scaffold; silk protein; tissue engineering