Are fat acids of human milk impacted by pasteurization and freezing?
The Human Milk Bank undergo human milk to pasteurization, followed by storage in a freezer at -18° C for up to six months to thus keep available the stocks of this product in maternal and infant hospitals. The objective of this study was to evaluate the effects of processing on the lipid fraction of human milk. A sample of human milk was obtained from a donor and was subdivided into ten sub-samples that was subjected to the following treatments: LC = raw milk; T0 = milk after pasteurization; T30 = milk after pasteurization and freezing for 30 days; T60 = milk after pasteurization and freeze for 60 days, and so on every 30 days until T240 = milk after pasteurization and freezing for 240 days, with 3 repetitions for each treatment. Lipids were extracted, methylated and fatty acid profiles determined by gas chromatography. The fatty acids were characterized by nuclear magnetic resonance and functional groups were identified by infrared spectroscopy. There were variations in the concentration of fatty acids. For unsaturated fatty acids there was increasing trend in their concentrations. The IR and NMR analyze characterized and identified functional groups presents in fatty acids.
Faculty of Agronomy and Veterinary Medicine, Laboratory of Food Analysis University of Brasilia.. luizborgo@gmail.com.
Luiz Antônio
LA
Borgo
Department of Nutrition. University of Brasilia.. luizborgo@gmail.com.
Wilma Maria
WM
Coelho Araújo
Institute of Chemistry, University of Brasilia. Brazil.. luizborgo@gmail.com.
Maria Hosana
MH
Conceição
Institute of Chemistry, University of Brasilia. Brazil.. luizborgo@gmail.com.
Inês
I
Sabioni Resck
Faculty of Agronomy and Veterinary Medicine, Laboratory of Food Analysis University of Brasilia.. luizborgo@gmail.com.
Márcio Antonio
MA
Mendonça
D002849:Chromatography, Gas; D003080:Cold Temperature; D015925:Cryopreservation; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry*; D005260:Female; D005519:Food Preservation; D006801:Humans; D017784:Milk Banks; D008895:Milk, Human / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D061352:Pasteurization; D013055:Spectrophotometry, Infrared
D005227:Fatty Acids