[Single-laboratory validation study of simple and simultaneous determination method for pesticide residues in meat by LC-MS/MS].
We performed a single-laboratory validation study of a simple and simultaneous determination method for pesticide residues in meat using LC-MS/MS. Water was added to the sample and the mixture was homogenized. Next, pesticides were extracted with acetonitrile containing 1 vol% formic acid using a homogenizer, and salted out with magnesium sulfate, trisodium citrate and sodium chloride. After centrifugation, the acetonitrile layer was made up to standard volume and analyzed by LC-MS/MS. This method was assessed by performing recovery tests in retail bovine, swine and chicken muscle samples spiked with the 132 pesticides at the levels of 0.01 and 0.04 μg/g. Among them, 125 pesticides satisfied the Japanese method validation guideline criteria in bovine, 120 in swine and 127 in chicken.
Tokyo Metropolitan Institute of Public Health.
Takeo
T
Sasamoto
Maki
M
Kobayashi
Naoko
N
Sakai
Kyoko
K
Kamijo
Harunori
H
Ootani
Masaki
M
Hayashi
Itoko
I
Baba
Chikako
C
Hanashiro
Ichiro
I
Takano
D000097:Acetonitriles; D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D017408:Guidelines as Topic; D007564:Japan; D008460:Meat / Q000032:analysis*; D010573:Pesticide Residues / Q000302:isolation & purification*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D000097:Acetonitriles; D010573:Pesticide Residues; C032159:acetonitrile