Occurrence and molecular identification of Giardia duodenalis from stray cats in Guangzhou, southern China.
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Guochao
G
Zheng
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Wei
W
Hu
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Yuanjia
Y
Liu
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Qin
Q
Luo
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Liping
L
Tan
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Guoqing
G
Li
D000818:Animals; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D002681:China; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000469:parasitology / Q000662:veterinary*; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S
China; Giardia duodenalis; cat; multilocus genotyping; zoonotic assemblage