Ovariectomy does not exacerbate the negative effects of sleep deprivation on synaptic plasticity in rats.
In our previous work, we found that female rats showed more cognitive impairment than male rats following 72h sleep deprivation (SD). Here, we compared the intact female with ovariectomized (OVX) rats to assess the potential modulatory effects of endogenous female sex hormones against the 48h SD-induced cognitive and synaptic modulations. The multiple platform method was applied for SD induction and spatial performances were determined using Morris water maze (MWM) task. Early longterm potentiation (E-LTP) was evaluated in area CA1 of the hippocampus and PCR and western blotting assays were employed to assess hippocampal BDNF gene and protein expression. To reveal any influence of sleep loss on stress level, we also measured the plasma corticosterone levels of animals. Regardless of reproductive status, SD significantly impaired short-term memory and LTP, but did not significantly change the BDNF expression in the hippocampus. The corticosterone levels were decreased in both intact and OVX female rats following SD. These findings suggest that depletion of female sex steroid hormones does not lead to any heightened responsivity of female animals to the negative effects of SD on cognitive and synaptic functions.
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran; Quchan Higher Health Education Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Vhajali@yahoo.com.
Vahid
V
Hajali
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Vahid
V
Sheibani
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Iran.
Saeed E
SE
Mahani
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Iran.
Zahra
Z
Hajializadeh
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Mohammad
M
Shabani
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Iran.
Hamzeh P
HP
Aliabadi
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran; Faculty of Medicine, Ardebil University of Medical Sciences, Ardebil, Iran.
Hakimeh
H
Saadati
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Khadijeh
K
Esmaeilpour
D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000473:pathology; D018782:Maze Learning; D008570:Memory, Short-Term / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D010052:Ovariectomy; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012892:Sleep Deprivation / Q000097:blood / Q000473:pathology* / Q000503:physiopathology*; D065853:Spatial Learning / Q000502:physiology; D013550:Swimming
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D003345:Corticosterone
Brain derived neurotropic factor; Long-term potentiation; Ovariectomy; Sleep deprivation; Spatial memory