Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-D-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.
1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan.
Yoshihiko
Y
Onda
1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan [3] Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan.
Keiichi
K
Mochida
Integrated Genome Informatics Research Unit, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan.
Takuhiro
T
Yoshida
Integrated Genome Informatics Research Unit, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan.
Tetsuya
T
Sakurai
School of Biological Sciences, University of Adelaide, Australia.
Roger S
RS
Seymour
United Graduate School of Agricultural Science, Iwate University, Morioka, Japan.
Yui
Y
Umekawa
Department of Biology and Botanical Garden, University of Crete, Heraklion, Greece.
Stergios Arg
SA
Pirintsos
1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan.
Kazuo
K
Shinozaki
Cryobiofrontier Research Center, Iwate University, Morioka, Japan.
Kikukatsu
K
Ito
D031058:Arum / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D035264:Flowers / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009812:Odorants / Q000032:analysis; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013729:Terpenes / Q000378:metabolism; D014166:Transferases / Q000235:genetics / Q000378:metabolism; D000099188:Alternative Oxidase
D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D013729:Terpenes; D014166:Transferases; D000099188:Alternative Oxidase; C109461:deoxyxylulose-5-phosphate synthase