Carbohydrate conjugation through microwave-assisted functionalization of single-walled carbon nanotubes using perfluorophenyl azides.
Carbohydrate-functionalized single-walled carbon nanotubes (SWNTs) were synthesized using microwave-assisted reaction of perfluorophenyl azide with the nanotubes. The results showed that microwave radiation provides a rapid and effective means to covalently attach carbohydrates to SWNTs, producing carbohydrate-SWNT conjugates for biorecognition. The carbohydrate-functionalized SWNTs were furthermore shown to interact specifically with cognate carbohydrate-specific proteins (lectins), resulting in predicted recognition patterns. The carbohydrate-presenting SWNTs constitute a new platform for sensitive protein- or cell recognition, which pave the way for glycoconjugated carbon nanomaterials in biorecognition applications.
KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden.
Na
N
Kong
KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden.
Manishkumar R
MR
Shimpi
KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden. Electronic address: ramstrom@kth.se.
Olof
O
Ramström
KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden; Department of Chemistry, University of Massachusetts Lowell, 1 University Ave., Lowell, MA 01854, United States. Electronic address: Mingdi_Yan@uml.edu.
Mingdi
M
Yan
D001386:Azides / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D037102:Lectins / Q000378:metabolism; D008872:Microwaves; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
D001386:Azides; D002241:Carbohydrates; D006845:Hydrocarbons, Fluorinated; D037102:Lectins; D037742:Nanotubes, Carbon; C556136:perfluorophenyl azide
Carbohydrate; Covalent functionalization; Lectin; Microwave; Perfluorophenyl azide; Single-walled carbon nanotubes