Molecularly imprinted cryogels for human serum albumin depletion.
Molecularly imprinted polymers can be used for the selective capture of a target molecule from complex medium. Cryogels novel matrices, which characterized by their supermacropores that makes their use advantageous when studying with biological samples. By combining high selectivity of the molecular imprinting approach with using cryogel as a base polymer, in this protocol, preparation of the albumin-imprinted cryogels is described. This material is a useful candidate for the selective albumin depletion from the human serum sample prior to the detailed proteomic analysis.
Biochemistry Division, Department of Chemistry, Hacettepe University, Chemistry Department, Ankara, 06800, Turkey.
Muge
M
Andac
Igor Yu
IY
Galaev
Handan
H
Yavuz
Adil
A
Denizli
D059985:Cryogels / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D054802:Molecular Imprinting; D010649:Phenylalanine / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000737:chemistry; D040901:Proteomics; D012709:Serum Albumin / Q000737:chemistry* / Q000302:isolation & purification*
D059985:Cryogels; D011073:Polyamines; D012709:Serum Albumin; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate; D010649:Phenylalanine