Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.
In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31±0.32g/L) and mannitol (10.12±0.17g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44±0.54g/L succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24±0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Bing
B
Bai
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Jie-min
JM
Zhou
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Mao-hua
MH
Yang
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yi-lan
YL
Liu
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Xiao-hui
XH
Xu
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: jmxing@home.ipe.ac.cn.
Jian-min
JM
Xing
D004926:Escherichia coli / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods; D005947:Glucose / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D012624:Seaweed / Q000737:chemistry*; D019802:Succinic Acid / Q000302:isolation & purification / Q000378:metabolism*
D008353:Mannitol; D019802:Succinic Acid; D005947:Glucose
Escherichia coli; Laminaria japonica; Macroalgae; Mannitol; Succinic acid