Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach.
A comparative proteomic approach was carried out between two contrasting alfalfa cultivars, nonomu (NM-801; salt tolerant) and vernal (VN; salt intolerant) in terms of salt tolerance. Seedlings were subjected to salt stress (50 and 100 mM NaCl) for three days. Several physiological parameters (leaf water, chlorophyll, root Na(+), K(+), and Ca(2+)) and root proteome profile were analyzed. Comparison of physiological status revealed that NM-801 is more tolerant to salt than VN. Eighty three differentially expressed proteins were found on 2-DE maps, of which 50 were identified by MALDI-TOF or MALDI-TOF/TOF mass spectrometry. These proteins were involved in ion homeostasis, protein turnover and signaling, protein folding, cell wall components, carbohydrate and energy metabolism, reactive oxygen species regulation and detoxification, and purine and fatty acid metabolism. The comparative proteome analysis showed that 33 salt-responsive proteins were significantly changed in both cultivars, while 17 (14 in VN and 3 in NM-801) were cultivar-specific. Peroxidase, protein disulfide-isomerase, NAD synthetase, and isoflavone reductase were up-regulated significantly only in NM-801 in all salt concentrations. In addition, we identified novel proteins including NAD synthetase and biotin carboxylase-3 that were not reported previously as salt-responsive. Taken together, these results provide new insights of salt stress tolerance in alfalfa.
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Md Atikur
MA
Rahman
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea; National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh.
Iftekhar
I
Alam
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yong-Goo
YG
Kim
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Na-Young
NY
Ahn
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Sung-Hyun
SH
Heo
Division of Life Science, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.
Dong-Gi
DG
Lee
Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
Gongshe
G
Liu
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: hyun@gnu.ac.kr.
Byung-Hyun
BH
Lee
D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D000455:Medicago sativa / Q000145:classification / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D055049:Salt Tolerance; D055051:Salt-Tolerant Plants / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*; D013045:Species Specificity; D013312:Stress, Physiological
D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride
Alfalfa; Forage; Legume; Proteome; Salt stress