Aptamer-modified magnetic beads in affinity separation of proteins.
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
Institut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Callinstr. 5, 30167, Hannover, Germany.
Guohong
G
Zhu
Johanna-Gabriela
JG
Walter
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D043182:Carboxylesterase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000737:chemistry; D059346:Magnets / Q000737:chemistry*; D008863:Microspheres; D011551:Pseudomonas fluorescens / Q000201:enzymology
D052157:Aptamers, Nucleotide; D006639:Histidine; D043182:Carboxylesterase; C469486:Ces1c protein, mouse