MicroRNA-328 inhibits proliferation of human melanoma cells by targeting TGFβ2.
Some microRNAs (miRNAs) have been shown to act as oncogenes or tumor suppressor genes in human melanomas. miR-328 is upregulated in blood cells of melanoma patients compared to in healthy controls. This suggests a role for miR-328 in melanoma that warrants investigation. In this study, we demonstrated miR-328 levels to be dramatically decreased in human melanoma cell lines. Moreover, forced expression of miR-328 inhibited proliferation and induced G1-phase arrest of the SK-MEL-1 melanoma cell line. We identified TGFβ2 as a direct target gene for miR-328 using a fluorescent reporter assay and western blotting. Levels of TGFβ2 were dramatically increased in human melanoma cell lines and were inversely correlated with the miR-328 expression level. Our findings provide new insights into the mechanisms of human melanoma development, indicating that miR-328 has therapeutic potential for this disease.
Department of Dermatology, Guangzhou First People's Hospital, Guangzhou, Guangdong Province, China E-mail : gzsdyrmyynsyypfk@126.com.
Jing-Rong
JR
Li
Jian-Qin
JQ
Wang
Qing
Q
Gong
Rui-Hua
RH
Fang
Yun-Long
YL
Guo
D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053781:Transforming Growth Factor beta2 / Q000235:genetics / Q000378:metabolism*
C548170:MIRN328 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C509041:TGFB2 protein, human; D053781:Transforming Growth Factor beta2