Effects of dietary virgin olive oil polyphenols: hydroxytyrosyl acetate and 3, 4-dihydroxyphenylglycol on DSS-induced acute colitis in mice.
Hydroxytyrosol, a polyphenolic compound from extra virgin olive oil (EVOO) has exhibited an improvement in a model of DSS-induced colitis. However, other phenolic compounds present such as hydroxytyrosyl acetate (HTy-Ac) and 3,4-dihydroxyphenylglycol (DHPG) need to be explored to complete the understanding of the overall effects of EVOO on inflammatory colon mucosa. This study was designed to evaluate the effect of both HTy-Ac and DHPG dietary supplementation in the inflammatory response associated to colitis model. Six-week-old mice were randomized in four dietary groups: sham and control groups received standard diet, and other two groups were fed with HTy-Ac and DHPG, respectively, at 0.1%. After 30 days, all groups except sham received 3% DSS in drinking water for 5 days followed by a regime of 5 days of water. Acute inflammation was evaluated by Disease Activity Index (DAI), histology and myeloperoxidase (MPO) activity. Colonic expression of iNOS, COX-2, MAPKs, NF-kB and FOXP3 were determined by western blotting. Only HTy-Ac-supplemented group showed a significant DAI reduction as well as an improvement of histological damage and MPO. COX-2 and iNOS protein expression were also significantly reduced. In addition, this dietary group down-regulated JNK phosphorylation and prevented the DSS-induced nuclear translocation level of p65. However, no significant differences were observed in the FOXP3 expression. These results demonstrated, for the first time, that HTy-Ac exerts an antiinflammatory effect on acute ulcerative colitis. We concluded that HTy-Ac supplement might provide a basis for developing a new dietary strategy for the prevention of ulcerative colitis.
Department of Pharmacology, Faculty of Pharmacy, University of Seville. Electronic address: fidalgo@us.es.
Susana
S
Sánchez-Fidalgo
Department of Pharmacology, Faculty of Pharmacy, University of Seville.
Isabel
I
Villegas
Department of Pharmacology, Faculty of Pharmacy, University of Seville.
Marina
M
Aparicio-Soto
Department of Pharmacology, Faculty of Pharmacy, University of Seville.
Ana
A
Cárdeno
Department of Pharmacology, Faculty of Pharmacy, University of Seville.
Ma Ángeles
MÁ
Rosillo
Department of Organic Chemistry, Faculty of Pharmacy, University of Seville.
Alejandro
A
González-Benjumea
Department of Organic Chemistry, Faculty of Pharmacy, University of Seville.
Azucena
A
Marset
Department of Organic Chemistry, Faculty of Pharmacy, University of Seville.
Óscar
Ó
López
Department of Organic Chemistry, Faculty of Pharmacy, University of Seville.
Inés
I
Maya
Department of Organic Chemistry, Faculty of Pharmacy, University of Seville.
José G
JG
Fernández-Bolaños
Department of Pharmacology, Faculty of Pharmacy, University of Seville.
Catalina
C
Alarcón de la Lastra
D000085:Acetates / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity*; D005260:Female; D020935:MAP Kinase Signaling System; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D000069463:Olive Oil / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism
D000085:Acetates; D002396:Catechols; D016328:NF-kappa B; D000069463:Olive Oil; C524604:hydroxytyrosyl acetate; D008734:Methoxyhydroxyphenylglycol; D016264:Dextran Sulfate; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C010117:3,4-dihydroxyphenylglycol
3,4-dihydroxyphenylglycol; Hydroxytyrosyl acetate; Inflammation; NF-kB; Ulcerative colitis