Leeuwenhoekiella polynyae sp. nov., isolated from a polynya in western Antarctica.
A Gram-stain-negative, motile by gliding, rod-shaped bacterial strain, designated SOJ2014-1(T) was isolated from surface water of a polynya in the Antarctic Ocean. A comparative 16S rRNA gene sequence analysis showed that strain SOJ2014-1(T) belongs to the genus Leeuwenhoekiella and is most closely related to Leeuwenhoekiella marinoflava DSM 3653(T) (97.5% 16S rRNA gene sequence similarity). The G+C content of the genomic DNA of strain SOJ2014-1(T) was 38.8 mol%. Its predominant cellular fatty acids were summed feature 3 (composed of C16 : 1ω6c and/or C16 : 1ω7c), iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G and summed feature 9 (composed of iso-C17 : 1ω9c and/or 10-methyl C16 : 0). DNA-DNA relatedness between strain SOJ2014-1(T) and close relatives, L. marinoflava DSM 3653(T) and Leeuwenhoekiella aequorea LMG 22550(T), was below 49%. The respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The strain grew at 0-35 °C (optimum, 25 °C) with 0-14.0% (w/v) NaCl (optimum, 1.0-5.0%). It was strictly aerobic and had different carbohydrate utilization traits compared with L. marinoflava DSM 3653(T). Based on the phenotypic, chemotaxonomic and phylogenetic analyses, strain SOJ2014-1(T) is proposed as a representative of a novel species, Leeuwenhoekiella polynyae. The type strain is SOJ2014-1(T) ( =KCTC 42185(T) =JCM 30387(T)).
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Ok-Ja
OJ
Si
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
So-Jeong
SJ
Kim
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Man-Young
MY
Jung
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Seon-Bin
SB
Choi
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Jong-Geol
JG
Kim
Microbial Resource Center/KCTC, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Song-Gun
SG
Kim
Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.
Seong Woon
SW
Roh
Korea Polar Research Institute, Incheon 406-840, Republic of Korea.
SangHoon
S
Lee
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Sung-Keun
SK
Rhee
D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6