Casein Kinase 2 Negatively Regulates Abscisic Acid-Activated SnRK2s in the Core Abscisic Acid-Signaling Module.
SnRK2 kinases, PP2C phosphatases and the PYR/PYL/RCAR receptors constitute the core abscisic acid (ABA) signaling module that is thought to contain all of the intrinsic properties to self-regulate the hormone signal output. Here we identify Casein Kinase (CK)2 as a novel negative regulator of SnRK2. CK2 phosphorylates a cluster of conserved serines at the ABA box of SnRK2, increasing its binding to PP2C and triggering protein degradation. Consequently, CK2 action has implications on SnRK2 protein levels, as well as kinase activity and its response to abiotic stimuli.
Centre for Research in Agricultural Genomics, Parc de Recerca UAB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain. Electronic address: bvigmm@cid.csic.es.
Belmiro
B
Vilela
Centre for Research in Agricultural Genomics, Parc de Recerca UAB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
Elena
E
Nájar
Centre for Research in Agricultural Genomics, Parc de Recerca UAB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
Victoria
V
Lumbreras
ISV - Institut de Sciences du Végétal, CNRS, bat 23, avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
Jeffrey
J
Leung
Centre for Research in Agricultural Genomics, Parc de Recerca UAB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
Montserrat
M
Pagès
D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D015398:Signal Transduction; D003313:Zea mays / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D000040:Abscisic Acid; D047390:Casein Kinase II; D010749:Phosphoprotein Phosphatases
abiotic stress; maize; protein phosphorylation