[Redox-dependent ferric oxide nanoparticles loaded with doxorubicin and their influence on the functions of mitochondria].
The spectral, fluorescent and functional properties of ferric oxide and ferric hydroxide nanoparticles loaded with doxorubicin and stabilized with citric acid or lysine were studied in comparison with free doxorubicin. Their effect on the opening of calcium-induced mitochondrial pore and the possibility of the controlled release of doxorubicin under the influence of redox stimuli were investigated. The data show that the effect of nanoparticles on mitochondria depends on the type of a stabilizer. The spectral and fluorescence methods used allow us to estimate the presence or absence of free doxorubicin in solution of nanoparticles and the:concentration of bound doxorubicin. It is shown that the dithiotreitol and glutathione increase the amplitude of absorption and fluorescence of doxorubicin during incubation with nanoparticles. It is assumed that this effect may be associated with the reduction of the oxidized iron by thiols with subsequent release of doxorubicin.
T A
TA
Fedotcheva
A G
AG
Akopdzhanov
N L
NL
Shimanovskiĭ
V V
VV
Mingalev
V V
VV
Banin
A A
AA
Zemlianaia
V V
VV
Teplova
N I
NI
Fedotcheva
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology*; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005290:Ferric Compounds; C000499:ferric oxide; D004317:Doxorubicin