Elevated expression of esterase and cytochrome P450 are related with lambda-cyhalothrin resistance and lead to cross resistance in Aphis glycines Matsumura.
A resistant strain of the Aphis glycines Matsumura (CRR) has developed 76.67-fold resistance to lambda-cyhalothrin compared with the susceptible (CSS) strain. Synergists piperonyl butoxide (PBO), S,S,S-Tributyltrithiophosphate (DEF) and triphenyl phosphate (TPP) dramatically increased the toxicity of lambda-cyhalothrin to the resistant strain. Bioassay results indicated that the CRR strain had developed high levels of cross-resistance to chlorpyrifos (11.66-fold), acephate (8.20-fold), cypermethrin (53.24-fold), esfenvalerate (13.83-fold), cyfluthrin (9.64-fold), carbofuran (14.60-fold), methomyl (9.32-fold) and bifenthrin (4.81-fold), but did not have cross-resistance to chlorfenapyr, imidacloprid, diafenthiuron, abamectin. The transcriptional levels of CYP6A2-like, CYP6A14-like and cytochrome b-c1 complex subunit 9-like increased significantly in the resistant strain than that in the susceptible. Similar trend were observed in the transcripts and DNA copy number of CarE and E4 esterase. Overall, these results demonstrate that increased esterase hydrolysis activity, combined with elevated cytochrome P450 monooxygenase detoxicatication, plays an important role in the high levels of lambda-cyhalothrin resistance and can cause cross-resistance to other insecticides in the CRR strain.
College of Plant Science, Jilin University, Changchun 130062, China.
Jinghui
J
Xi
College of Plant Science, Jilin University, Changchun 130062, China.
Yiou
Y
Pan
College of Plant Science, Jilin University, Changchun 130062, China.
Rui
R
Bi
Department of Entomology, China Agricultural University, Beijing 100193, China.
Xiwu
X
Gao
College of Plant Science, Jilin University, Changchun 130062, China; Department of Entomology, China Agricultural University, Beijing 100193, China.
Xuewei
X
Chen
College of Plant Science, Jilin University, Changchun 130062, China.
Tianfei
T
Peng
Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China.
Min
M
Zhang
College of Plant Science, Jilin University, Changchun 130062, China.
Hua
H
Zhang
College of Plant Science, Jilin University, Changchun 130062, China.
Xiaoyue
X
Hu
College of Plant Science, Jilin University, Changchun 130062, China. Electronic address: shangqingli@163.com.
Qingli
Q
Shang
D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*
D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017160:cypermethrin; C099952:bifenthrin; D003577:Cytochrome P-450 Enzyme System; D004950:Esterases; C052570:cyfluthrin; C037304:cyhalothrin; C017690:fenvalerate
Aphis glycines; Cross resistance; Cytochrome P450 monooxygenase; Esterase; Insecticide resistance; Synergism