Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-l-glutamic acid-modified bacterial magnetosomes.
To solve the problem of synthesized magnetic nanoparticles in cancer therapy, a new drug delivery system synthesized from bacteria was used to load cytosine arabinoside (Ara-C). Genipin (GP) and poly-l-glutamic acid (PLGA) were selected as dual cross-linkers. The preparation and characterization of Ara-C-loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ABMs-P), as well as their in vitro antitumor effects, were all investigated. Transmission electron micrographs (TEM) and Fourier transform infrared (FTIR) spectroscopy suggested that Ara-C could be bound to the membrane of BMs modified by GP-PLGA. The diameters of the BMs and ABMs-P were 42.0±8.6 nm and 74.9±8.2 nm, respectively. The zeta potential revealed that the nanoparticles were stable. Moreover, this system exhibited optimal drug-loading properties and long-term release behavior. The optimal encapsulation efficiency and drug-loading were 64.1%±6.6% and 38.9%±2.4%, respectively, and ABMs-P could effectively release 90% Ara-C within 40 days, without the release of an initial burst. In addition, in vitro antitumor experiments elucidated that ABMs-P is cytotoxic to HL-60 cell lines, with an inhibition rate of 95%. The method of coupling drugs on BMs using dual cross-linkers is effective, and our results reveal that this new system has potential applications for drug delivery in the future.
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China ; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Yuan-Gang
YG
Liu
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Qing-Lei
QL
Dai
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China ; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Shi-Bin
SB
Wang
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Qiong-Jia
QJ
Deng
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China ; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Wen-Guo
WG
Wu
College of Chemical Engineering, Huaqiao University, Xiamen, People's Republic of China ; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People's Republic of China.
Ai-Zheng
AZ
Chen
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003561:Cytarabine / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D039823:Iridoids / Q000737:chemistry*; D055944:Magnetosomes / Q000737:chemistry*
D000970:Antineoplastic Agents; D004337:Drug Carriers; D039823:Iridoids; D003561:Cytarabine; D018698:Glutamic Acid; C007834:genipin
drug delivery; dual cross-linkers; magnetosomes; nanoparticle