Commercially Available Rapid Methods for Detection of Selected Food-borne Pathogens.
Generally, the enumeration and isolation of food-borne pathogens is performed using culture-dependent methods. These methods are sensitive, inexpensive, and provide both qualitative and quantitative assessment of the microorganisms present in a sample, but these are time-consuming. For this reason, researchers are developing new techniques that allow detection of food pathogens in shorter period of time. This review identifies commercially available methods for rapid detection and quantification of Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Shiga toxin-producing Escherichia coli in food samples. Three categories are discussed: immunologically based methods, nucleic acid-based assays, and biosensors. This review describes the basic mechanism and capabilities of each method, discusses the difficulties of choosing the most convenient method, and provides an overview of the future challenges for the technology for rapid detection of microorganisms.
a Department of Food Science , Pennsylvania State University , University Park , Pennsylvania , USA.
Wladir B
WB
Valderrama
a Department of Food Science , Pennsylvania State University , University Park , Pennsylvania , USA.
Edward G
EG
Dudley
a Department of Food Science , Pennsylvania State University , University Park , Pennsylvania , USA.
Stephanie
S
Doores
a Department of Food Science , Pennsylvania State University , University Park , Pennsylvania , USA.
Catherine N
CN
Cutter
D015374:Biosensing Techniques; D005516:Food Microbiology / Q000379:methods*; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay; D008089:Listeria monocytogenes / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000302:isolation & purification; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013997:Time Factors
Food safety; Listeria monocytogenes; Salmonella spp; Shiga toxin-producing Escherichia coli (STEC); Staphylococcus aureus