Ratiometric iridium(III) complex-based phosphorescent chemodosimeter for Hg(2+) applicable in time-resolved luminescence assay and live cell imaging.
A novel iridium(III) complex-based probe Ir4-1 has been designed and synthesized conveniently by incorporating the chemodosimeter into phosphorescent luminophor, which displayed ratiometric luminescence change from yellowish-green to reddish-yellow only toward Hg(2+) ions in aqueous media via desulfurization and intramolecular cyclization with a broad pH range of 5-10. The phosphorescent chemodosimeter could eliminate effectively the signal interference from the short-lived fluorescent background, and the signal-to-noise ratio of the detection was improved distinctly by using time-resolved photoluminescence technique. Furthermore, the mechanism of phosphoresce change of the chemodosimeter was analyzed in detail by time-dependent density functional theory (TD-DFT) calculations, and the probe with long-wavelength emission could be applied to label cells and monitor intracellular Hg(2+) effectively by luminescence ratio imaging.
†Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Jiaxi
J
Ru
‡School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Xu
X
Chen
‡School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Liping
L
Guan
†Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Xiaoliang
X
Tang
‡School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Chunming
C
Wang
‡School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Yue
Y
Meng
†Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Guolin
G
Zhang
†Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Weisheng
W
Liu
D045744:Cell Line, Tumor; D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D007495:Iridium / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging; D009942:Organometallic Compounds / Q000737:chemistry*; D011789:Quantum Theory; D013997:Time Factors
D049408:Luminescent Agents; D009942:Organometallic Compounds; D007495:Iridium; D008628:Mercury