Intravital microscopic methods to evaluate anti-inflammatory effects and signaling mechanisms evoked by hydrogen sulfide.
Hydrogen sulfide (H2S) is an endogenous gaseous signaling molecule with potent anti-inflammatory properties. Exogenous application of H2S donors, administered either acutely during an inflammatory response or as an antecedent preconditioning intervention that invokes the activation of anti-inflammatory cell survival programs, effectively limits leukocyte rolling, adhesion and emigration, generation of reactive oxygen species, chemokine and cell adhesion molecule expression, endothelial barrier disruption, capillary perfusion deficits, and parenchymal cell dysfunction and injury. This chapter focuses on intravital microscopic methods that can be used to assess the anti-inflammatory effects exerted by H2S, as well as to explore the cellular signaling mechanisms by which this gaseous molecule limits the aforementioned inflammatory responses. Recent advances include use of intravital multiphoton microscopy and optical biosensor technology to explore signaling mechanisms in vivo.
Harry S. Truman Veterans Administration Hospital, Cardiology, Columbia, Missouri, USA.
Mozow Y
MY
Zuidema
Department of Medical Pharmacology and Physiology, School of Medicine, One Hospital Drive, University of Missouri, Columbia, Missouri, USA; Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri, USA. Electronic address: korthuisr@health.missouri.edu.
Ronald J
RJ
Korthuis
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D017078:Laser-Doppler Flowmetry; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012709:Serum Albumin; D015398:Signal Transduction
D000894:Anti-Inflammatory Agents, Non-Steroidal; C518288:FITC-albumin; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D018799:Intercellular Adhesion Molecule-1; D051547:Heme Oxygenase-1; D016650:Fluorescein-5-isothiocyanate; D006862:Hydrogen Sulfide
Adhesion molecule expression; Chemokine expression; Hydrogen sulfide; Inflammation; Intravital microscopy; Ischemia; Leukocyte adhesion; Leukocyte emigration; Leukocyte rolling; Microvascular permeability; No-reflow; Preconditioning; Reactive oxygen and nitrogen species; Reperfusion