An in vivo swine study for xeno-grafts of calcium sulfate-based bone grafts with human dental pulp stem cells (hDPSCs).
The purpose of this in vivo study was to evaluate the effect of human dental pulp stem cells (hDPSCs) on various resorbable calcium sulfate/calcium phosphate bone grafts in bone regeneration. Granular particles of calcium sulfate dehydrate (CSD), α-calcium sulfate hemihydrate/amorphous calcium phosphate (α-CSH/ACP), and CSD/β-tricalcium phosphates (β-TCP) were prepared for in vitro dissolution and implantation test. The chemical compositions of specimen residues after dissolution test were characterized by XRD. The ratios of new bone formation for implanted grafts/hDPSCs were evaluated using mandible bony defect model of Lanyu pig. All the graft systems exhibited a similar two-stage dissolution behavior and phase transformation of poor crystalline HAp. Eight weeks post-operation, the addition of hDPSCs to various graft systems showed statistically significant increasing in the ratio of new bone formation (p<0.05). Null hypothesis of hDPSCs showing no scaffold dependence in bone regeneration was rejected. The results suggest that the addition of hDPSCs to calcium sulfate based xenografts could enhance the bone regeneration in the bony defect.
Department of Veterinary Medicine, National Taiwan University, Taipei 110, Taiwan, ROC; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC.
Tzong-fu
TF
Kuo
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC; Dental Department, Wan-Fang Hospital, Taipei Medical University, Taipei 110, Taiwan, ROC; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC.
Sheng-Yang
SY
Lee
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC.
Hong-Da
HD
Wu
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC.
Malosi
M
Poma
Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC.
Yu-Wei
YW
Wu
Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC; School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC. Electronic address: yang820065@tmu.edu.tw.
Jen-Chang
JC
Yang
D000818:Animals; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000494:pharmacology; D002133:Calcium Sulfate / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D034421:Sus scrofa; D014183:Transplantation, Heterologous; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; C519480:amorphous calcium phosphate; C485817:beta-tricalcium phosphate; D002133:Calcium Sulfate
Dental implant; Human dental pulp stem cells (hDPSCs); Xeno-grafts