Magnetically responsive siliceous frustules for efficient chemotherapy.
In the present investigation, curcumin loaded magnetically active frustules have been reported. The diatoms were cultured and frustules were obtained by chemical and thermal processes. The frustules were rendered magnetically active by incorporation of iron oxide nanoparticle using two different methods involving ferrofluid (CMDM-F) and in situ synthesis (CMDM-I) of iron oxide nanoparticle. These CMDM prepared by two techniques were characterized using FT-IR and vibrating sample magnetometer (VSM) analyses. Particle size and potential were measured using the Malvern Zetasizer. Scanning electron microscopy (SEM) was utilized for studying the surface morphology of CMDM, and in addition to this elemental analysis was also performed for confirming the presence of iron. The cell viability assay was carried out using the HeLa cell line. SEM images showed a change in surface morphology of diatoms before and after rendering magnetic activity. Cell viability assay revealed that CMDM-F had reasonably high cytotoxicity (60.2%) compared to Curcumin (42.1%), DM (1.9%), CDM (44.8%), and CMDM-I (59.9). Both, CMDM-F and CMDM-I showed improved cytotoxicity when compared with pure curcumin. The overall study suggests that the developed CMDM could be utilized as a potential carrier to deliver cargo for efficient chemotherapy.
Department of Biotechnology, School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
Vivek S
VS
Javalkote
H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
Abhijeet P
AP
Pandey
Department of Biotechnology, School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
Pravin R
PR
Puranik
H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India. Electronic address: pkdesh@rediffmail.com.
Prashant K
PK
Deshmukh
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology; D017377:Diatoms / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D000970:Antineoplastic Agents; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D003474:Curcumin
Cell viability; Cervical cancer; Curcumin; Diatoms; Ferrofluid