Doing that thing that scientists do: A discovery-driven module on protein purification and characterization for the undergraduate biochemistry laboratory classroom.
In traditional introductory biochemistry laboratory classes students learn techniques for protein purification and analysis by following provided, established, step-by-step procedures. Students are exposed to a variety of biochemical techniques but are often not developing procedures or collecting new, original data. In this laboratory module, students develop research skills through work on an original research project and gain confidence in their ability to design and execute an experiment while faculty can enhance their scholarly pursuits through the acquisition of original data in the classroom laboratory. Students are prepared for a 6-8 week discovery-driven project on the purification of the Escherichia coli cytidylate kinase (CMP kinase) through in class problems and other laboratory exercises on bioinformatics and protein structure analysis. After a minimal amount of guidance on how to perform the CMP kinase in vitro enzyme assay, SDS-PAGE, and the basics of protein purification, students, working in groups of three to four, develop a protein purification protocol based on the scientific literature and investigate some aspect of CMP kinase that interests them. Through this process, students learn how to implement a new but perhaps previously worked out procedure to answer their research question. In addition, they learn the importance of keeping a clear and thorough laboratory notebook and how to interpret their data and use that data to inform the next set of experiments. Following this module, students had increased confidence in their ability to do basic biochemistry techniques and reported that the "self-directed" nature of this lab increased their engagement in the project.
Department of Chemistry, Vassar College, Poughkeepsie, New York, 12604.
Teresa A
TA
Garrett
Department of Biology, Vassar College, Poughkeepsie, New York, 12604.
Joseph
J
Osmundson
Department of Biology, Vassar College, Poughkeepsie, New York, 12604.
Marisa
M
Isaacson
Department of Chemistry, Vassar College, Poughkeepsie, New York, 12604.
Jennifer
J
Herrera
D001671:Biochemistry / Q000193:education* / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry / Q000302:isolation & purification*
D029968:Escherichia coli Proteins; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase
active learning; biochemistry laboratory; discovery-driven; enzyme purification; inquiry based teaching; integration of research into undergraduate teaching