Dicentric assay: inter-laboratory comparison in Indian laboratories for routine and triage applications.
An Inter-Laboratory Comparison (ILC) study on Dicentric Chromosome Assay (DCA) was carried out between two Indian biodosimetry labs. Human peripheral blood samples exposed to 10 different doses of X-rays up to 5Gy were shared between the labs to generate calibration data. Validation of calibration curves was done by dose estimation of coded samples exposed to X- or gamma radiation. Reliability of the DCA data for triage application was evaluated by scoring 20, 50 and 100 metaphases in the dose range of 0.5-3.0Gy. No significant difference was observed between labs regarding the established calibration data as well as the DCA triage dose assessments. Scoring of 20 metaphases (MP) was adequate to detect radiation exposure of >2Gy whereas 50 MP were sufficient to determine exposures of 0.5Gy. Both labs performed the DCA in a reliable manner and made the first step in setting up a biodosimetry network in India.
Radiological Safety Division, Indira Gandhi Center for Atomic Research, Kalpakkam, Tamilnadu, India.
D
D
Bakkiam
Sri Ramachandra University, Porur, Chennai 600116, Tamilnadu, India.
M
M
Bhavani
Radiological Safety Division, Indira Gandhi Center for Atomic Research, Kalpakkam, Tamilnadu, India. Electronic address: aakgen1@igcar.gov.in.
A Arul
AA
Anantha Kumar
Radiological Safety Division, Indira Gandhi Center for Atomic Research, Kalpakkam, Tamilnadu, India.
Swetha
S
Sonwani
Sri Ramachandra University, Porur, Chennai 600116, Tamilnadu, India.
P
P
Venkatachalam
Radiological Safety Division, Indira Gandhi Center for Atomic Research, Kalpakkam, Tamilnadu, India.
K
K
Sivasubramanian
Radiological Safety Division, Indira Gandhi Center for Atomic Research, Kalpakkam, Tamilnadu, India.
B
B
Venkatraman
D001681:Biological Assay / Q000592:standards*; D002138:Calibration; D002869:Chromosome Aberrations / Q000528:radiation effects*; D003955:Diagnostic Tests, Routine / Q000592:standards; D004307:Dose-Response Relationship, Radiation; D007194:India; D015162:Micronucleus Tests / Q000592:standards*; D011785:Quality Assurance, Health Care / Q000592:standards*; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000592:standards*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016037:Single-Blind Method; D014218:Triage / Q000592:standards*
Biodosimetry; Dicentric assay; Inter-laboratory comparison; Triage dose estimation