New monoclonal antibodies specific for 1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole.
1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole (AM694) is one of the synthetic cannabinoids and an illegal drug in Japan. It is important to generate a monoclonal antibody (MAb) against AM694 for use in the rapid and sensitive detection of the drug. Two monoclonal antibodies, named HN0124 (IgG1) and NK0504 (IgG1), were obtained, which were possibly effective for detecting AM694 and its derivatives. The cross-reactive ability of these MAbs was evaluated using a competitive enzyme-linked immunosorbent assay. In the results, both of these antibodies recognize 1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole, 1-(5-fluoropentyl)-3-(3-iodobenzoyl)indole, 1-(5-fluoropentyl)-3-(4-iodobenzoyl)indole. Forty nmol/L AM694 can be detected using HN0124 MAb. Thus, MAbs produced in this study could be considered a useful tool for the detection of AM694.
Bioscience Technology Development Office, R&D Division, Panasonic Co. , Souraku-gun, Japan .
Hiroshi
H
Nakayama
Noriko
N
Kenjyou
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D001667:Binding, Competitive; D063386:Cannabinoid Receptor Agonists / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D013287:Illicit Drugs / Q000032:analysis*; D007114:Immunization; D018796:Immunoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000737:chemistry*; D007211:Indoles / Q000032:analysis*; D007564:Japan; D057230:Limit of Detection; D051379:Mice
C568441:AM 694; D000911:Antibodies, Monoclonal; D063386:Cannabinoid Receptor Agonists; D006241:Haptens; D013287:Illicit Drugs; D018796:Immunoconjugates; D007074:Immunoglobulin G; D007211:Indoles; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin