MR VIGS: microRNA-based virus-induced gene silencing in plants.
In plants, microRNA (miRNA)-based virus-induced gene silencing, dubbed MR VIGS, is a powerful technique to delineate the biological functions of genes. By targeting to a specific sequence, miRNAs can knock down expression of genes with fewer off-target effects. Here, using a modified Cabbage leaf curling virus (CaLCuV) and Tobacco rattle virus (TRV) as vectors, we describe two virus-based miRNA expression systems to perform MR VIGS for plant functional genomics assays.
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, 16 Xuelin Street, Jianggan, Zhejiang, Hangzhou, China.
Weiwei
W
Chen
Qi
Q
Zhang
Junhua
J
Kong
Feng
F
Hu
Bin
B
Li
Chaoqun
C
Wu
Cheng
C
Qin
Pengcheng
P
Zhang
Nongnong
N
Shi
Yiguo
Y
Hong
D016960:Agrobacterium tumefaciens / Q000821:virology; D001937:Brassica / Q000235:genetics / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology
D035683:MicroRNAs; D010940:Plant Proteins