Nucleic acid from beans extracted by ethanediamine magnetic particles.
Ethanediamine magnetite nanoparticles (EDAMPs) were used as adsorbents to isolate genomic DNA from various bean-species. A "single-pot" preparation of EDAMPs was described. Further characterization, including transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), thermo-gravimetric analysis (TGA), X-ray powder diffraction (XRD) and Fourier Transform Infrared Spectrophotometer (FT-IR) were used to demonstrate the efficiency of this simple and general identification method. The EDAMPs provided excellent yields of genomic DNA. The isolated DNA was suitable for use in further applications by the Polymerase Chain Reaction (PCR) for the detection of Genetically Modified (GM) food and non-GM food. The EDAMPs are effective for bioseparation applications.
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Fengxiang
F
Qie
State Key Laboratory of Chemical Resource Engineering, College of Science, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Guoxin
G
Zhang
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Jianxuan
J
Hou
State Key Laboratory of Chemical Resource Engineering, College of Science, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Xiaoming
X
Sun
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Shi-Zhong
SZ
Luo
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Tianwei
T
Tan
Beans; Electrophoresis; Ethanediamine magnetite nanoparticles; Genomic DNA