In vitro degradation and cell response of calcium carbonate composite ceramic in comparison with other synthetic bone substitute materials.
The robust calcium carbonate composite ceramics (CC/PG) can be acquired by fast sintering calcium carbonate at a low temperature (650 °C) using a biocompatible, degradable phosphate-based glass (PG) as sintering agent. In the present study, the in vitro degradation and cell response of CC/PG were assessed and compared with 4 synthetic bone substitute materials, calcium carbonate ceramic (CC), PG, hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ceramics. The degradation rates in decreasing order were as follows: PG, CC, CC/PG, β-TCP, and HA. The proliferation of rat bone mesenchymal stem cells (rMSCs) cultured on the CC/PG was comparable with that on CC and PG, but inferior to HA and β-TCP. The alkaline phosphatase (ALP) activity of rMSCs on CC/PG was lower than PG, comparable with β-TCP, but higher than HA. The rMSCs on CC/PG and PG had enhanced gene expression in specific osteogenic markers, respectively. Compared to HA and β-TCP, the rMSCs on the CC/PG expressed relatively lower level of collagen I and runt-related transcription factor 2, but showed more considerable expression of osteopontin. Although CC, PG, HA, and β-TCP possessed impressive performances in some specific aspects, they faced extant intrinsic drawbacks in either degradation rate or mechanical strength. Based on considerable compressive strength, moderate degradation rate, good cell response, and being free of obvious shortcoming, the CC/PG is promising as another choice for bone substitute materials.
Department of Biomedical Engineering, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
Fupo
F
He
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Jing
J
Zhang
Department of Biomedical Engineering, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
Fanwen
F
Yang
Department of Biomedical Engineering, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
Jixiang
J
Zhu
Department of Biomedical Engineering, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
Xiumei
X
Tian
Department of Biomedical Engineering, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China. Electronic address: xmchenw@126.com.
Xiaoming
X
Chen
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D002119:Calcium Carbonate / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology*; D019245:Compressive Strength; D005786:Gene Expression Regulation / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D014961:X-Ray Diffraction
D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D000469:Alkaline Phosphatase; D002119:Calcium Carbonate
Calcium carbonate; Calcium phosphate; Cell response; In vitro degradation; Phosphate-based glass