Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp.
Identification and characterization of the RNAi-related genes is the key to understanding RNAi mechanism in shrimp. In this study, we have identified and characterized a novel putative RNA helicase gene, Mj-mov-10 from the kuruma shrimp, Marsupenaeus japonicus and its implication in shrimp RNAi was demonstrated. The full-length Mj-mov-10 gene contained 3536bp, including 239 bp of 5'UTR, 2895 bp of the open reading frame (ORF) and 402bp of 3'UTR, respectively. An ORF of Mj-mov-10 could be translated to a 109-kDa protein which consists of a single helicase core domain containing seven signature motifs of the RNA helicase superfamily-1. Mj-MOV-10 protein shared 47% and 40% identity with mammalian MOV-10 and plant SDE3, respectively. Expression of Mj-mov-10 gene was significantly up-regulated upon dsRNA and white spot syndrome virus (WSSV) challenge. In vivo gene knockdown of Mj-mov-10 resulted in an increase of a susceptibility of shrimp to WSSV infection. Our results implied the functional significance of Mj-MOV-10 in dsRNA-mediated gene silencing and antiviral defense mechanism in shrimp.
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
Amnat
A
Phetrungnapha
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.
Hidehiro
H
Kondo
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.
Ikuo
I
Hirono
Institute of Molecular Biosciences, Mahidol University, Phutthamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand; Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Phayathai, Bangkok 10400, Thailand.
Sakol
S
Panyim
Institute of Molecular Biosciences, Mahidol University, Phutthamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand. Electronic address: chalermporn.ong@mahidol.ac.th.
Chalermporn
C
Ongvarrasopone
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics*; D020365:RNA Helicases / Q000235:genetics*; D034622:RNA Interference
D060829:Arthropod Proteins; D020365:RNA Helicases
Double stranded RNA; RISC; RNAi; Shrimp; White spot syndrome virus