Functional analysis of GmCPDs and investigation of their roles in flowering.
The onset of floral development is a pivotal switch in the life of soybean. Brassinosteroids (BRs), a group of steroidal phytohormones with essential roles in plant growth and development, are associated with flowering induction. Genes involved in BR biosynthesis have been studied to a great extent in Arabidopsis, but the study of these genes has been limited in soybean. In this study, four CPD homologs (GmCPDs) catalyzing BR synthesis were isolated from soybean. Transcripts were mainly confined to cotyledons and leaves and were down-regulated in response to exogenous BR. Bioinformatic analysis showed strong sequence and structure similarity between GmCPDs and AtCPD as well as CPDs of other species. Overexpression of GmCPDs in an Arabidopsis BR-deficient mutant rescued the phenotype by restoring the biosynthesis pathway, revealing the functional roles of each GmCPDs in. Except for the rescue of root development, leaf expansion and plant type architecture, GmCPDs in expression also complemented the late flowering phenotype of Arabidopsis mutants deficient in CPD. Further evidence in soybean plants is that the expression levels of GmCPDs in are under photoperiod control in Zigongdongdou, a photoperiod-sensitive variety, and show a sudden peak upon floral meristem initiation. Together with increased GmCPDs in expression in the leaves and cotyledons of photoperiod-insensitive early-maturity soybean, it is clear that GmCPDs in contribute to flowering development and are essential in the early stages of flowering regulation.
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China; College of Plant Science, Jilin University, Changchun, 130062, Jilin, China.
Miao
M
Wang
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Xin
X
Xu
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Xinxin
X
Zhang
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Shi
S
Sun
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Cunxiang
C
Wu
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Wensheng
W
Hou
College of Plant Science, Jilin University, Changchun, 130062, Jilin, China.
Qingyu
Q
Wang
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Tianfu
T
Han
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D060406:Brassinosteroids / Q000096:biosynthesis / Q000494:pharmacology; D003001:Cloning, Molecular; D035264:Flowers / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D009154:Mutation; D017440:Photoperiod; D010802:Phylogeny; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D013025:Glycine max / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D010940:Plant Proteins; D013250:Steroid Hydroxylases