Start codon targeted (SCoT) polymorphism reveals genetic diversity in wild and domesticated populations of ramie (Boehmeria nivea L. Gaudich.), a premium textile fiber producing species.
Twenty-four start codon targeted (SCoT) markers were used to assess genetic diversity and population structure of indigenous, introduced and domesticated ramie (Boehmeria nivea L. Gaudich.). A total of 155 genotypes from five populations were investigated for SCoT polymorphism, which produced 136 amplicons with 87.5% polymorphism. Polymorphism information content and resolving power of the SCoT markers were 0.69 and 3.22, respectively. The Indian ramie populations exhibited high SCoT polymorphism (> 50%), high genetic differentiation (GST = 0.27) and moderate gene flow (Nm = 1.34). Analysis of molecular variance identified significant differences for genetic polymorphism among the populations explaining 13.1% of the total variation. The domesticated population exhibited higher genetic polymorphism and heterozygosity compared to natural populations. Cluster analysis supported population genetic analysis and suggested close association between introduced and domesticated genotypes. The present study shows effectiveness of employing SCoT markers in a cross pollinated heterozygous species like Boehmeria, and would be useful for further studies in population genetics, conservation genetics and cultivar improvement.
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
Pratik
P
Satya
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
Maya
M
Karan
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
Sourav
S
Jana
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
Sabyasachi
S
Mitra
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
Amit
A
Sharma
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.
P G
PG
Karmakar
ICAR-National Institute of Research on Jute and Allied Fibre Technology, Kolkata 700040, India.
D P
DP
Ray
AMOVA; Boehmeria nivea; Genetic diversity; India; Population structure; Ramie; SCoT