MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro.
Previous work from our laboratory demonstrated the existence of miRNAs in human follicular fluid. In the current study, we have sought to identify miRNAs that might affect oocyte/embryo quality in patients undergoing intracytoplasmic sperm injection and to investigate their roles in in vitro fertilization outcomes in mouse oocytes. 53 samples were classified as Group 1 (high quality) if the day-3 embryos had seven and more cells or as Group 2 (low quality) if the embryos had six and fewer cells. TaqMan Human microRNAs cards and qRT-PCR were performed to verify differently expressed miRNAs. The function of the corresponding miRNA was investigated in mouse oocytes by injecting them with miRNA-inhibitor oligonucleotides. We found that hsa-miR-320a and hsa-miR-197 had significantly higher expression levels in the Group 1 follicular fluids than in Group 2 (p = 0.0073 and p = 0.008, respectively). Knockdown of mmu-miR-320 in mouse oocytes strongly decreased the proportions of MII oocytes that developed into two-cell and blastocyst stage embryos (p = 0.0048 and p = 0.0069, respectively). Wnt signaling pathway components had abnormal expression level in miR-320 inhibitor-injected oocytes. This study provides the first evidence that miRNAs in human follicular fluid are indicative of and can influence embryo quality.
1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Ruizhi
R
Feng
1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Qing
Q
Sang
Guangdong No.2 provincial people's hospital, Guangzhou, China.
Yan
Y
Zhu
Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Wei
W
Fu
Shanghai Institute of Planned Parenthood Research, Shanghai, China.
Miao
M
Liu
Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Yan
Y
Xu
Shanghai Institute of Planned Parenthood Research, Shanghai, China.
Huijuan
H
Shi
1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Yao
Y
Xu
1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Ronggui
R
Qu
Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
Renjie
R
Chai
Department of Physiology/Endocrinology, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.
Ruijin
R
Shao
State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.
Li
L
Jin
1] Institutes of Biomedical Sciences, Fudan University, Shanghai, China [2] Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China.
Lin
L
He
Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Xiaoxi
X
Sun
1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Lei
L
Wang
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism; D034622:RNA Interference; D060449:Wnt Signaling Pathway
C572916:MIRN320 microRNA, human; D035683:MicroRNAs