A sufficient role of MHC class I molecules on hepatocytes in anti-plasmodial activity of CD8 (+) T cells in vivo.
Although CD8(+) T cells are shown to mediate the protective immunity against the liver stages of malaria parasites in mice, whether the direct presentation of malaria antigen by major histocompatibility complex (MHC) class I molecules expressed on the liver of infected host is required for anti-plasmodial activity of CD8(+) T cells is still unknown. Presently, there is only one CD8(+) epitope, SYVPSAEQI, derived from the circumsporozoite protein of Plasmodium yoelii (PyCS), that mediates anti-malarial protection and is presented in the context of a K(d) molecule. Therefore, to investigate the mode of anti-plasmodial activity of CD8+ T cells, we have previously generated C57BL/6 transgenic (Tg) mice, in which a K(d) molecule is expressed only on hepatocyte (Alb-K(d)) or dendritic cell (DC; CD11c-K(d)), by using albumin promoter or CD11c promoter, respectively. We have also generated MHC-I-K(d) Tg mice, which express the K(d) molecule under the MHC class I (MHC-I) promoter, as a positive control. From splenocytes collected from CD11c-K(d) Tg mice immunized with a synthetic peptide, SYVPSAEQI, which corresponds to the CD8(+) T-cell epitope of PyCS, emulsified in incomplete Freund's adjuvant , a PyCS-specific CD8(+) T-cell line was generated. This PyCS-specific CD8(+)T-cell line was then adoptively transferred into a cohort of either MHC-K(d) Tg or Alb-K(d) Tg mice listed above, as well as wild-type C57BL/6 mice. Then both transferred and non-transferred mice were challenged with live malaria parasites. We found that the adoptive transfer of a PyCS-specific CD8(+) T-cell line resulted in a significant inhibition of the parasite burden in the liver of Alb-K(d) Tg, as well as MHC-I-K(d) Tg mice, but not of C57BL/6 mice. These results indicate that the K(d) molecule expressed by hepatocytes is sufficient in mediating the anti-plasmodial activity of PyCS-specific CD8(+) T cells in vivo.
HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center - The Rockefeller University New York, NY, USA.
Jing
J
Huang
HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center - The Rockefeller University New York, NY, USA.
Tiffany
T
Tsao
Department of Pathology, New York University New York, NY, USA.
Min
M
Zhang
HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center - The Rockefeller University New York, NY, USA.
Urvashi
U
Rai
HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center - The Rockefeller University New York, NY, USA.
Moriya
M
Tsuji
HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center - The Rockefeller University New York, NY, USA.
Xiangming
X
Li
CD8+ T cell; MHC class I; liver; malaria; transgenic mouse