Comparative genomics of Cluster O mycobacteriophages.
Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages--Corndog, Catdawg, Dylan, Firecracker, and YungJamal--designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.
Department of Biology, James Madison University, Harrisonburg, Virginia, United States of America.
Steven G
SG
Cresawn
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Welkin H
WH
Pope
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Deborah
D
Jacobs-Sera
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Charles A
CA
Bowman
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Daniel A
DA
Russell
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Rebekah M
RM
Dedrick
Department of Biology, Baylor University, Waco, Texas, United States of America.
Tamarah
T
Adair
Department of Biology, Gonzaga University, Spokane, Washington, United States of America.
Kirk R
KR
Anders
Center for Life Sciences Education, The Ohio State University, Columbus, Ohio, United States of America.
Sarah
S
Ball
Biology Department, Illinois Wesleyan University, Bloomington, Illinois, United States of America.
David
D
Bollivar
Center for Life Sciences Education, The Ohio State University, Columbus, Ohio, United States of America.
Caroline
C
Breitenberger
Department of Microbiology & Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
Sandra H
SH
Burnett
Biology Department, Seton Hill University, Greensburg, Pennsylvania, United States of America.
Kristen
K
Butela
Biology Department, Carthage College, Kenosha, Wisconsin, United States of America.
Deanna
D
Byrnes
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
Sarah
S
Carzo
Department of Chemistry & Biochemistry, Providence College, Providence, Rhode Island, United States of America.
Kathleen A
KA
Cornely
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
Trevor
T
Cross
Biology Department, College of Idaho, Caldwell, Idaho, United States of America.
Richard L
RL
Daniels
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
David
D
Dunbar
School of Sciences, University of Louisiana at Monroe, Monroe, Louisiana, United States of America.
Ann M
AM
Findley
School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, United States of America.
Chris R
CR
Gissendanner
Department of Biological Sciences & Geology, Queensborough Community College, Bayside, New York, United States of America.
Urszula P
UP
Golebiewska
Department of Molecular, Cell & Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.
Grant A
GA
Hartzog
Department of Natural Sciences, Del Mar College, Corpus Christi, Texas, United States of America.
J Robert
JR
Hatherill
Department of Biological Sciences, University of North Texas, Denton, Texas, United States of America.
Lee E
LE
Hughes
School of Life Sciences, University of QwaZulu-Natal, Durban, South Africa.
Chernoh S
CS
Jalloh
Department of Molecular, Cell & Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.
Carla
C
De Los Santos
Department of Molecular, Cell & Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.
Kevin
K
Ekanem
School of Life Sciences, University of QwaZulu-Natal, Durban, South Africa.
Sphindile L
SL
Khambule
Department of Biology, Western Kentucky University, Bowling Green, Kentucky, United States of America.
Rodney A
RA
King
Department of Biology, Saint Joseph's University, Philadelphia, Pennsylvania, United States of America.
Christina
C
King-Smith
Department of Biology, University of Wisconsin-River Falls, River Falls, Wisconsin, United States of America.
Karen
K
Klyczek
Department of Biology, Gettysburg College, Gettysburg, Pennsylvania, United States of America.
Greg P
GP
Krukonis
Department of Math & Computer Science, Wilkes University, Wilkes Barre, Pennsylvania, United States of America.
Christian
C
Laing
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Jonathan S
JS
Lapin
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.
A Javier
AJ
Lopez
School of Life Sciences, University of QwaZulu-Natal, Durban, South Africa.
Sipho M
SM
Mkhwanazi
Department of Molecular & Biomedical Sciences, University of Maine Honors College, Orono, Maine, United States of America.
Sally D
SD
Molloy
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
Deborah
D
Moran
KwaZulu-Natal Research Institute for Tuberculosis & HIV, Durban, South Africa.
Vanisha
V
Munsamy
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Eddie
E
Pacey
Department of Biological Sciences, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.
Ruth
R
Plymale
Department of Biology, Gonzaga University, Spokane, Washington, United States of America.
Marianne
M
Poxleitner
Department of Biological Sciences, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America.
Nathan
N
Reyna
Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America.
Joel F
JF
Schildbach
Department of Biology, Hope College, Holland, Michigan, United States of America.
Joseph
J
Stukey
Department of Biology, Brown University, Providence, Rhode Island, United States of America.
Sarah E
SE
Taylor
Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of America.
Vassie C
VC
Ware
School of Life Sciences, University of QwaZulu-Natal, Durban, South Africa.
Amanda L
AL
Wellmann
Biology Department, The College of St. Scholastica, Duluth, Minnesota, United States of America.
Daniel
D
Westholm
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
Donna
D
Wodarski
Department of Biology, Cabrini College, Radnor, Pennsylvania, United States of America.
Michelle
M
Zajko
School of Life Sciences, University of QwaZulu-Natal, Durban, South Africa.
Thabiso S
TS
Zikalala
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Roger W
RW
Hendrix
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Graham F
GF
Hatfull
D004279:DNA, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D009160:Mycobacteriophages / Q000235:genetics*; D010802:Phylogeny
D004279:DNA, Viral