[Examination of processed vegetable foods for the presence of common DNA sequences of genetically modified tomatoes].
The contamination of processed vegetable foods with genetically modified tomatoes was investigated by the use of qualitative PCR methods to detect the cauliflower mosaic virus 35S promoter (P35S) and the kanamycin resistance gene (NPTII). DNA fragments of P35S and NPTII were detected in vegetable juice samples, possibly due to contamination with the genomes of cauliflower mosaic virus infecting juice ingredients of Brassica species and soil bacteria, respectively. Therefore, to detect the transformation construct sequences of GM tomatoes, primer pairs were designed for qualitative PCR to specifically detect the border region between P35S and NPTII, and the border region between nopaline synthase gene promoter and NPTII. No amplification of the targeted sequences was observed using genomic DNA purified from the juice ingredients. The developed qualitative PCR method is considered to be a reliable tool to check contamination of products with GM tomatoes.
Research and Development Division, KAGOME Co., Ltd.
Mamiko
M
Kitagawa
Kosuke
K
Nakamura
Kazunari
K
Kondo
Shoji
S
Ubukata
Hiroshi
H
Akiyama
D001483:Base Sequence / Q000235:genetics*; D017796:Caulimovirus / Q000235:genetics; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D030841:Food, Genetically Modified; D007613:Kanamycin Resistance / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D018744:DNA, Plant; D004279:DNA, Viral