Structural studies of the C-terminal 19-peptide of serum amyloid A and its Pro → Ala variants interacting with human cystatin C.
Serum amyloid A (SAA) is a multifunctional acute-phase protein whose concentration in serum increases markedly following a number of chronic inflammatory and neoplastic diseases. Prolonged high SAA level may give rise to reactive systemic amyloid A (AA) amyloidosis, where the N-terminal segment of SAA is deposited as amyloid fibrils. Besides, recently, well-documented association of SAA with high-density lipoprotein or glycosaminoglycans, in particular heparin/heparin sulfate (HS), and specific interaction between SAA and human cystatin C (hCC), the ubiquitous inhibitor of cysteine proteases, was proved. Using a combination of selective proteolytic excision and high-resolution mass spectrometry, a hCC binding site in the SAA sequence was determined as SAA(86-104). The role of this SAA C-terminal fragment as a ligand-binding locus is still not clear. It was postulated important in native SAA folding and in pathogenesis of AA amyloidosis. In the search of conformational details of this SAA fragment, we did its structure and affinity studies, including its selected double/triple Pro → Ala variants. Our results clearly show that the SAA(86-104) 19-peptide has rather unordered structure with bends in its C-terminal part, which is consistent with the previous results relating to the whole protein. The results of affinity chromatography, fluorescent ELISA-like test, CD and NMR studies point to an importance of proline residues on structure of SAA(86-104). Conformational details of SAA fragment, responsible for hCC binding, may help to understand the objective of hCC-SAA complex formation and its importance for pathogenesis of reactive amyloid A amyloidosis.
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Martyna
M
Maszota
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Natalia
N
Karska
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Marta
M
Spodzieja
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Jerzy
J
Ciarkowski
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Aleksandra S
AS
Kołodziejczyk
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-952, Gdańsk, Poland.
Sylwia
S
Rodziewicz-Motowidło
Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Kładki 24, 80-822, Gdańsk, Poland.
Paulina
P
Czaplewska
D000409:Alanine / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D055316:Cystatin C / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011392:Proline / Q000737:chemistry; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D000685:Serum Amyloid A Protein / Q000737:chemistry* / Q000378:metabolism
C525030:CST3 protein, human; D055316:Cystatin C; D010455:Peptides; D020033:Protein Isoforms; D000685:Serum Amyloid A Protein; D011392:Proline; D000409:Alanine
ELISA; NMR; SAA; conformation; hCC; protein-protein interaction