Protective effect of allicin against glycidamide-induced toxicity in male and female mice.
Acrylamide is known to be a neurotoxic, genotoxic, and carcinogenic compound. Glycidamide has a close relationship to the toxic mechanism of acrylamide. In order to explore the toxic mechanism of acrylamide, we further discussed the effects of oral administration of allicin on glycidamide-induced toxicity by determining the hematological parameters like AST, ALT, LDH, BUN, creatinine, ROS, and 8-OHdG, and biochemical parameters such as MDA, MPO, SOD, GST and GSH in the kidney, liver, brain and lung of male and female mice for the first time. We found that the same dose of glycidamide had more toxic effects and damage effects to the mice compared to the previous study of acrylamide. It could markedly increase the level of AST, ALT, LDH, BUN, ROS, 8-OHdG, MDA, MPO while decrease the SOD, GST and GSH. However, our data showed the oral administered allicin with a concentration of 5, 10, and 20 mg/kg b.w./day could significantly decrease the damage indexes of AST, ALT, LDH, BUN, ROS, 8-OHdG, MDA, and MPO, while increase the antioxidant indicators of SOD, GST and GSH. Thus allicin could be used as an effective dietary supplement for the chemoprevention of glycidamide genotoxicity internally, and to prevent the tissue damage and toxicity induced by glycidamide.
College of Quartermaster Technology, Jilin University, 130062 Changchun, China. yuanyuan1024@gmail.com.
En-Ting
ET
Wang
Dong-Yan
DY
Chen
Huang-You
HY
Liu
Hai-Yang
HY
Yan
Yuan
Y
Yuan
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000276:immunology; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004852:Epoxy Compounds / Q000633:toxicity*; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013441:Sulfinic Acids / Q000008:administration & dosage*; D016896:Treatment Outcome
D000975:Antioxidants; D004220:Disulfides; D004852:Epoxy Compounds; D017382:Reactive Oxygen Species; D013441:Sulfinic Acids; C006452:allicin; C071834:glycidamide