Boosting of activity enhancement of K(+)-responsive quadruplex hammerhead ribozyme.
Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K(+)via quadruplex formation of embedded r(GGA)3GG, were developed. Different strategies were applied to suppress basal activity when K(+) is absent. As a result, the activity enhancement upon the addition of K(+) has reached as high as 21-fold.
Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. katahira.masato.6u@kyoto-u.ac.jp nagata.takashi.6w@kyoto-u.ac.jp.
Yudai
Y
Yamaoki
Tsukasa
T
Mashima
Takashi
T
Nagata
Masato
M
Katahira
D015374:Biosensing Techniques; D002384:Catalysis; D002414:Cations, Monovalent; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D011188:Potassium / Q000032:analysis* / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D013379:Substrate Specificity
D002414:Cations, Monovalent; D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D011188:Potassium