[Proportion and significance of CD1d(hi)CD5⁺CD19⁺ regulatory B cell in peripheral blood of patients with neuromyelitis optica].
OBJECTIVE
To detect the proportion of CD1d(hi)CD5⁺CD19⁺ regulatory B cells (Bregs) in peripheral blood of the patients with neuromyelitis optica (NMO), and explore whether CD1d(hi)CD5⁺CD19⁺ Bregs can play a role as a biomarker in the diagnosis of NMO versus multiple sclerosis (MS).


METHODS
Flow cytometry was performed to detect the proportion of CD1d(hi)CD5⁺CD19⁺ Bregs in peripheral blood from 44 cases of NMO, 38 cases of MS, and 30 healthy controls. The serum level of aquaporin-4 antibody (AQP4-Ab) of patients with NMO was detected by indirect immunofluorescence assay.


RESULTS
The proportion of CD1d(hi)CD5⁺CD19⁺ Bregs in CD19⁺ B cells and lymphocytes was significantly lower in NMO group than in MS and control groups; however, there was no significant difference between MS group and control group. The proportion of CD1d(hi)CD5⁺CD19⁺ Bregs in CD19⁺ B cells and lymphocytes was lower in AQP4-Ab-positive NMO patients than in AQP4-Ab-negative NMO patients, and the difference was statistically significant.


CONCLUSIONS
CD1d(hi)CD5⁺CD19⁺ Bregs may be a biomarker in the differential diagnosis of NMO versus MS.
Department of Neurology in South Building, General Hospital of PLA, Beijing 100853, China.
Fen
F
Yang
Department of Neurology, General Hospital of PLA, Beijing 100853, China.
Dehui
D
Huang
Department of Neurology in South Building, General Hospital of PLA, Beijing 100853, China.
Chen
C
Cheng
Department of Neurology in South Building, General Hospital of PLA, Beijing 100853, China.
Weiping
W
Wu
D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis; D055610:Antigens, CD1d / Q000032:analysis; D060151:B-Lymphocytes, Regulatory / Q000166:cytology*; D001772:Blood Cell Count; D018956:CD5 Antigens / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009471:Neuromyelitis Optica / Q000097:blood* / Q000175:diagnosis; D055815:Young Adult
D018941:Antigens, CD19; D055610:Antigens, CD1d; D018956:CD5 Antigens