Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure.
Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zero-five years old. Host immunity and viral genetic variability are important factors that can make vaccine production difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment with the child's serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon alteration in absence and presence of human sera in individual clones of BR-85 sample.
Instituto de Ciências Biomédicas.
Claudia Trigo Pedroso
CT
Moraes
Instituto de Ciências Biomédicas.
Danielle Bruna Leal
DB
Oliveira
Instituto de Ciências Biomédicas.
Angelica Cristine Almeida
AC
Campos
Instituto de Ciências Biomédicas.
Patricia Alves
PA
Bosso
Instituto de Ciências Biomédicas.
Hildener Nogueira
HN
Lima
Instituto de Ciências Biomédicas.
Klaus Eberhard
KE
Stewien
Hospital Universitário, Universidade de São Paulo, São Paulo, SP, Brasil.
Alfredo Elias
AE
Gilio
Hospital Universitário, Universidade de São Paulo, São Paulo, SP, Brasil.
Sandra Elisabete
SE
Vieira
Instituto Butantan, São Paulo, SP, Brasil.
Viviane Fongaro
VF
Botosso
Instituto de Ciências Biomédicas.
Edison Luiz
EL
Durigon
D000914:Antibodies, Viral / Q000302:isolation & purification*; D001483:Base Sequence / Q000235:genetics; D002648:Child; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D009035:Mothers; D009154:Mutation; D017817:Nasal Lavage Fluid / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D010948:Viral Plaque Assay
D000914:Antibodies, Viral