Mineral phosphate solubilization by Streptomyces sp. CTM396 involves the excretion of gluconic acid and is stimulated by humic acids.
The actinomycetes isolates (128) which were taken from agricultural soil samples and collected near a rock phosphate processing unit were screened for mineral phosphate-solubilizing (MPS) ability. A significant MPS activity was observed for 30 isolates on various phosphate sources when grown in the National Botanical Research Institute's phosphate broth. CTM396 and CTM397 strains which showed the highest MPS abilities were identified by 16S rDNA sequencing as members of the genus Streptomyces. Their MPS activity was proved to be concomitant with a drop in pH due to the secretion of gluconic acid (GA). This was correlated with the simultaneous detection by PCR of genes gdh [encoding the glucose dehydrogenase (GDH) responsible for GA production from glucose] and pqq (involved in biosynthesis of the pyrroloquinoline quinone cofactor of GDH), as well as the highlighting of GHD enzyme activity, for the first time in a Streptomyces sp. strain producing GA. Furthermore, the 0.05% of humic acids proved to have a stimulatory effect on the growth and the ability of CTM396 to solubilize Gafsa rock phosphate. According to this study, it is possible to use humic acids and Gafsa rock phosphate in association with spores of ad hoc Streptomyces strains as natural and efficient amendments to improve plant growth with no need of costly and pollutant transformation of Gafsa rock phosphate.
Laboratoire de Microorganismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, BP '1177' 3018 Sfax, Tunisie.
Mounira Ben
MB
Farhat
Laboratoire de Microorganismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, BP '1177' 3018 Sfax, Tunisie.
Ines
I
Boukhris
Laboratoire de Microorganismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, BP '1177' 3018 Sfax, Tunisie hichem.chouayekh@cbs.rnrt.tn.
Hichem
H
Chouayekh
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal; D005942:Gluconates / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006812:Humic Substances; D006863:Hydrogen-Ion Concentration; D008903:Minerals / Q000378:metabolism*; D045542:PQQ Cofactor / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology; D012995:Solubility; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005942:Gluconates; D006812:Humic Substances; D008903:Minerals; D010710:Phosphates; D045542:PQQ Cofactor; D042843:Glucose 1-Dehydrogenase; C030691:gluconic acid
improving plant growth; mechanism of phosphate solubilization; solubilization activity of Streptomyces