The correlation between fibronectin adsorption and attachment of vascular cells on heparinized polycaprolactone membrane.
The heparin-immobilized polycaprolactone (PCL) membrane was developed to investigate the effect of heparin on the adsorption behaviors of serum proteins, in particular fibronectin (Fn), and the attachment of different types of vascular cells. The heparin was covalently immobilized onto aminolyzed PCL membrane by amidation reaction. The grafting amount of heparin increased from 0.35 μg/cm(2) to 1.31 μg/cm(2) when the aminolysis time was prolonged from 10 min to 60 min. The adhesion of endothelial cells (ECs) and smooth muscle cells (SMCs) on the heparinized PCL surface was significantly enhanced and reduced, respectively. The adsorption of Fn was strongly improved on the heparinized PCL surface than on the pristine PCL, and showed a positive correlation with the heparin density. Therefore, the adsorbed Fn takes a decisive role on the selective adhesion of ECs and the suppression of SMCs attachment.
MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Mingcong
M
Sun
MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Jun
J
Deng
MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: cygao@mail.hz.zj.cn.
Changyou
C
Gao
D000327:Adsorption / Q000187:drug effects; D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D005353:Fibronectins / Q000302:isolation & purification*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008567:Membranes, Artificial; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*
D000925:Anticoagulants; D001672:Biocompatible Materials; D005353:Fibronectins; D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D006493:Heparin
Endothelial cells; Fibronectin; Heparin; Polycaprolactone; Smooth muscle cells