Boronate affinity nanoparticles for RNA isolation.
In this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanoparticles were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FTIR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles.
Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey.
Aykut
A
Toprak
Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey.
Cansu
C
Görgün
Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey.
Cansu İlke
Cİ
Kuru
Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey.
Ceren
C
Türkcan
Adnan Menderes University, Koçarlı Vocational and Training School, 09010 Aydın, Turkey.
Murat
M
Uygun
Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey. Electronic address: sinanakgol@yahoo.co.uk.
Sinan
S
Akgöl
D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011102:Polyhydroxyethyl Methacrylate; D012331:RNA, Fungal / Q000302:isolation & purification*; D059027:Recycling; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature
D001897:Boronic Acids; D007093:Imidazoles; D012331:RNA, Fungal; D011102:Polyhydroxyethyl Methacrylate; C017424:2-imidazoline; C010686:benzeneboronic acid
Boronate affinity; Nanoparticle; RNA