Removal of iron by chelation with molecularly imprinted supermacroporous cryogel.
Iron chelation therapy can be used for the selective removal of Fe(3+) ions from spiked human plasma by ion imprinting. N-Methacryloyl-(L)-glutamic acid (MAGA) was chosen as the chelating monomer. In the first step, MAGA was complexed with the Fe(3+) ions to prepare the precomplex, and then the ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) [PHEMAGA-Fe(3+)] cryogel column was prepared by cryo-polymerization under a semi-frozen temperature of - 12°C for 24 h. Subsequently, the template, of Fe(3+) ions was removed from the matrix by using 0.1 M EDTA solution. The values for the specific surface area of the imprinted PHEMAGA-Fe(3+) and non-imprinted PHEMAGA cryogel were 45.74 and 7.52 m(2)/g respectively, with a pore size in the range of 50-200 μm in diameter. The maximum Fe(3+) adsorption capacity was 19.8 μmol Fe(3+)/g cryogel from aqueous solutions and 12.28 μmol Fe(3+)/g cryogel from spiked human plasma. The relative selectivity coefficients of ion-imprinted cryogel for Fe(3+)/Ni(2+) and Fe(3+)/Cd(2+) were 1.6 and 4.2-fold greater than the non-imprinted matrix, respectively. It means that the PHEMAGA-Fe(3+) cryogel possesses high selectivity to Fe(3+) ions, and could be used many times without significantly decreasing the adsorption capacity.
a Department of Chemistry , Hacettepe University , Beytepe, Ankara , Turkey.
Duygu
D
Çimen
a Department of Chemistry , Hacettepe University , Beytepe, Ankara , Turkey.|b Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe, Ankara , Turkey.
Ilgım
I
Göktürk
c Department of Chemistry Technology , Vocational School of Gerede , Bolu , Turkey.
Fatma
F
Yılmaz
D059985:Cryogels / Q000138:chemical synthesis / Q000737:chemistry*; D005971:Glutamates / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D016062:Porosity
D059985:Cryogels; D005971:Glutamates; D007502:Iron Chelating Agents; D008689:Methacrylates; C514131:methacryloylamidoglutamic acid; D007501:Iron
affinity; cryogel; iron removal; metal detoxification; molecular recognition