Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation.
Cell encapsulation in three-dimensional (3D) hydrogels can mimic native cell microenvironment and plays a major role in cell-based transplantation therapies. In this contribution, a novel in situ-forming hydrogel, Dex-l-DTT hydrogel ("l" means "linked-by"), by cross-linking glycidyl methacrylate derivatized dextran (Dex-GMA) and dithiothreitol (DTT) under physiological conditions, has been developed using thiol-Michael addition reaction. The mechanical properties, gelation process and degree of swelling of the hydrogel can be easily adjusted by changing the pH of phosphate buffer saline. The 3D cell encapsulation ability is demonstrated by encapsulating rat bone marrow mesenchymal stem cells (BMSCs) and NIH/3T3 fibroblasts into the in situ-forming hydrogel with maintained high viability. The BMSCs also maintain their differentiation potential after encapsulation. These results demonstrate that the Dex-l-DTT hydrogel holds great potential for biomedical field.
School of Science, State Key Laboratory for Mechanical Behaviour of Materials, Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China.
Zhen Qi
ZQ
Liu
School of Science, State Key Laboratory for Mechanical Behaviour of Materials, Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China.
Zhao
Z
Wei
School of Medicine, Yan'an University, Yan'an 716000, China; Department of Surgical Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
Xv Long
XL
Zhu
Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Guo You
GY
Huang
Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Feng
F
Xu
School of Science, State Key Laboratory for Mechanical Behaviour of Materials, Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China.
Jian Hai
JH
Yang
RIKEN 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.
Yoshihito
Y
Osada
Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, H-1084 Budapest, Nagyvárad tér 4, Hungary.
Miklós
M
Zrínyi
Department of Surgical Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
Jian Hui
JH
Li
School of Science, State Key Laboratory for Mechanical Behaviour of Materials, Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: chenym@mail.xjtu.edu.cn.
Yong Mei
YM
Chen
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004229:Dithiothreitol / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D044367:Phase Transition; D051381:Rats
D003432:Cross-Linking Reagents; D003911:Dextrans; D004852:Epoxy Compounds; D020100:Hydrogels; D008689:Methacrylates; C007870:glycidyl methacrylate; D004229:Dithiothreitol
3D cell encapsulation; Bone marrow mesenchymal stem cells; Dextran; Hydrogel; Thiol-Michael addition