Cryogels with affinity ligands as tools in protein purification.
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.
Norwegian Group AS, 8116, Stavanger, 4068, Norway.
Solmaz
S
Hajizadeh
Bo
B
Mattiasson
D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D059985:Cryogels / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000302:isolation & purification; D055767:Immobilized Proteins / Q000737:chemistry; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D059985:Cryogels; D055767:Immobilized Proteins; D008024:Ligands; D011506:Proteins; D003208:Concanavalin A; D011142:Polyvinyl Alcohol; D006735:Horseradish Peroxidase