Study of murine experimental Jorge Lobo's disease by analysis of peritoneal lavage cells and footpad histopathology: early versus chronic lesions.
The murine model of Jorge Lobo's disease is characterized by histological alterations similar to those seen in human disease, including a large number of viable fungi. This study evaluated the immune response of mice with early and late macroscopic lesions (5 and 13 months post-inoculation [p.i.], respectively) by the analysis of peritoneal lavage cells and footpad (FP) histology. The FP of mice were inoculated with 1 × 10(6) fungi (viability index of 41%). At 5 and 13 months p.i., the granuloma mainly consisted of macrophages and multinucleated giant cells, but a larger number of neutrophils was observed at 5 months and lymphocytes at 13 months. The number of fungi in the FP and fungal viability were 1.8 ± 1.1 × 10(6) fungi/ml and 38.5% at 5 months p.i. and 30.8 ± 11.7 × 10(6) fungi/ml and 9% at 13 months (P < .05). Higher production of H₂O₂, O₂(-), IL-10, and TNF-α were observed at 13 months (P < .05), but there was no significant difference in the production of NO, IL-2, IL-4, IL-12 and IFN-γ. The results showed significant differences between early and late lesions and support the use of BALB/c mice for evaluation of the different phases of infection.
Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil fmoreno@ilsl.br.
Fátima Regina
FR
Vilani-Moreno
Equipe Técnia de Microbiologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Sônia Maria Usó Ruiz
SM
Silva
Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Adriana Sierra Assencio Almeida
AS
Barbosa
Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Beatriz Gomes Carreira
BG
Sartori
Setor de Biotério, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Sílvia Cristina
SC
Barboza Pedrini
Equipe Técnica de Patologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Adauto José Ferreira
AJ
Nunes
Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Milca Ribeiro
MR
Saruhashi
Faculdade de Odontologia, Universidade de São Paulo, Bauru, SP, Brazil.
José Roberto Pereira
JR
Lauris
Equipe Técnia de Microbiologia, Instituto Lauro de Souza Lima, Bauru, SP, Brazil.
Suzana Madeira
SM
Diório
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003584:Cytological Techniques; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D005528:Foot / Q000473:pathology*; D005658:Fungi / Q000254:growth & development; D006099:Granuloma / Q000473:pathology; D006651:Histocytochemistry; D007962:Leukocytes / Q000276:immunology; D060368:Lobomycosis / Q000473:pathology*; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010533:Peritoneal Lavage
D016207:Cytokines; D009569:Nitric Oxide
Jorge Lobo's disease; cytokines; histopathology; mouse; peritoneal lavage