Antimicrobial behavior of biosynthesized silica-silver nanocomposite for water disinfection: a mechanistic perspective.
The biosynthesis of nano-silica silver nanocomposite (NSAgNC) and it is as antibacterial effect on gram-negative bacteria viz.Escherichia coli and Pseudomonas aeruginosa has been investigated for disinfection of water. The as-synthesized NSAgNC exhibited antibacterial activity in a dose dependent manner and ∼ 99.9% of E. coli and P. aeruginosa were killed at a concentration of 1.5 mg/mL of NSAgNC (5.1 wt% Ag) within 5h. The NSAgNC showed similar antibacterial activities both in oxic and anoxic conditions. The results further demonstrated that NSAgNC exhibited reactive oxygen species (ROS) independent "particle specific" antibacterial activity through multiple steps in absence of leached out Ag(+) ions. The initial binding of NSAgNC on the cell wall caused loss of cell membrane integrity and leakage of cytoplasmic materials. Inhibition of respiratory chain dehydrogenase by NSAgNC caused metabolic inactivation of the cells and affecting the cell viability. Genomic and proteomic studies further demonstrated the fragmentations of both plasmid and genomic DNA and down regulation of protein expression in NSAgNC treated cells, which leading to the cell death. Thus the biosynthesized NSAgNC has great potential as disinfectant for water purification while minimizing the toxic effects.
Bioproducts Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110001, India.
Thanusu
T
Parandhaman
Bioproducts Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India.
Anisha
A
Das
Bioproducts Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India.
B
B
Ramalingam
Polymer Division, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India.
Debasis
D
Samanta
Bioproducts Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India.
T P
TP
Sastry
Chemical Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110001, India. Electronic address: abmandal@hotmail.com.
Asit Baran
AB
Mandal
Bioproducts Laboratory, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai 600020, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110001, India. Electronic address: sujoy@clri.res.in.
Sujoy K
SK
Das
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D058491:Bacterial Load; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000235:genetics; D004203:Disinfection / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects; D000860:Hypoxia; D053761:Nanocomposites / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D040901:Proteomics; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D017382:Reactive Oxygen Species; D018030:Silver Compounds; D012822:Silicon Dioxide
DNA damage; Oxidative stress; Particle-specific activity; Reactive oxygen species; Silver nanomaterial; Water disinfection