Identification and characterization of a vitamin D₃ decomposition product bactericidal against Helicobacter pylori.
This study demonstrated that the vitamin D₃ decomposition product VDP1 exerts an antibacterial action against Helicobacter pylori but not against other bacteria. Treatment with VDP1 induced a collapse of cell membrane structures of H. pylori and ultimately lysed the bacterial cells. A unique dimyristoyl phosphatidylethanolamine in the membrane lipid compositions contributed to the interaction of VDP1 with H. pylori cells. In separate experiments, VDP1 had no influence on the viability of the human cancer cell lines MKN45 and T47D and lacked any vitamin D₃-like hormonal action against the latter. In both (1)H and (13)C NMR analyses, the spectra patterns of VDP1 corresponded with those of Grundmann's ketone. These results suggest that VDP1 (or Grundmann's ketone-type indene compound) may become a fundamental structure for the development of new antibacterial substances with selective bactericidal action against H. pylori.
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi. 329-0498, Japan.
Kouichi
K
Hosoda
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi. 329-0498, Japan.
Hirofumi
H
Shimomura
Faculty of Pharmaceutical Sciences, Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa. 245-0066, Japan.
Kiyofumi
K
Wanibuchi
Faculty of Pharmaceutical Sciences, Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa. 245-0066, Japan.
Hisashi
H
Masui
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi. 329-0498, Japan.
Avarzed
A
Amgalanbaatar
Department of Microbiology, Kitasato University School of Medicine, 1-15-1, Kitasato, Minami-ku, Sagamihara, Kanagawa. 252-0374, Japan.
Shunji
S
Hayashi
Faculty of Pharmaceutical Sciences, Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa. 245-0066, Japan.
Takashi
T
Takahashi
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi. 329-0498, Japan.
Yoshikazu
Y
Hirai
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002762:Cholecalciferol / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D016480:Helicobacter pylori / Q000187:drug effects* / Q000502:physiology*; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D002762:Cholecalciferol