Essential function of Aco2, a fusion protein of aconitase and mitochondrial ribosomal protein bL21, in mitochondrial translation in fission yeast.
A possible interaction between aconitase and a mitochondrial ribosomal protein was suggested in a genome-wide interactome study. In fission yeast Schizosaccharomyces pombe, the aco2(+) gene encodes a fusion protein between aconitase and a putative mitochondrial ribosomal protein bL21 (Mrpl49). Two types of aco2(+) transcripts are generated via alternative poly (A) site selection, producing both a single aconitase domain protein and the fusion form. The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial translation.
School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Soo-Jin
SJ
Jung
School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Youngdae
Y
Seo
School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Kyung-Chang
KC
Lee
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Daeyoup
D
Lee
School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: jhroe@snu.ac.kr.
Jung-Hye
JH
Roe
D000154:Aconitate Hydratase / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*
D024101:Mitochondrial Proteins; D012269:Ribosomal Proteins; D029702:Schizosaccharomyces pombe Proteins; C000597893:Aco2 protein, S pombe; D000154:Aconitate Hydratase
Dual function protein; Dual localized protein; Gene fusion; Mitochondrial targeting; Nuclear localization signal; Sub-cellular localization