Phytochemical investigation of Gynura bicolor leaves and cytotoxicity evaluation of the chemical constituents against HCT 116 cells.
Gynura bicolor (Compositae) is a popular vegetable in Asia and believed to confer a wide range of benefits including anti-cancer. Our previous findings showed that the ethyl acetate extract of G. bicolor possessed cytotoxicity and induced apoptotic and necrotic cell death in human colon carcinoma cells (HCT 116). A combination of column chromatography had been used to purify chemical constituents from the ethyl acetate and water extract of G. bicolor leaves. Eight chemical constituents 5-p-trans-coumaroylquinic acid (I), 4-hydroxybenzoic acid (II), rutin (III), kampferol-3-O-rutinoside (IV), 3,5-dicaffeoylquinic acid (V), kampferol-3-O-glucoside (VI), guanosine (VII) and chlorogenic acid (VIII) were isolated from G. bicolor grown in Malaysia. To our best knowledge, all chemical constituents were isolated for the first time from G. bicolor leaves except rutin (III). 3,5-dicaffeoylquinic acid (V), guanosine (VII) and chlorogenic acid (VIII) demonstrated selective cytotoxicity (selective index>3) against HCT 116 cancer cells compared to CCD-18Co human normal colon cells.
a Faculty of Science, Institute of Biological Sciences, University of Malaya , 50603 Kuala Lumpur , Malaysia.
Wuen Yew
WY
Teoh
b Phytochemistry Program, Natural Products Division, Forest Research Institute Malaysia , 52109 , Kepong , Selangor , Malaysia.
Hooi Poay
HP
Tan
b Phytochemistry Program, Natural Products Division, Forest Research Institute Malaysia , 52109 , Kepong , Selangor , Malaysia.
Sui Kiong
SK
Ling
c Biology Division, Centre for Foundation Studies in Science, University of Malaya , 50603 Kuala Lumpur , Malaysia.
Norhanom
N
Abdul Wahab
a Faculty of Science, Institute of Biological Sciences, University of Malaya , 50603 Kuala Lumpur , Malaysia.
Kae Shin
KS
Sim
D019659:Asteraceae / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification; D045325:HCT116 Cells / Q000187:drug effects; D006801:Humans; D008296:Malaysia; D015394:Molecular Structure; D010226:Parabens / Q000302:isolation & purification; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification; D012431:Rutin / Q000302:isolation & purification
D010226:Parabens; D064209:Phytochemicals; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002726:Chlorogenic Acid; D012431:Rutin; C038193:4-hydroxybenzoic acid; C100434:3,5-dicaffeoylquinic acid
3,5-dicaffeoylquinic acid; Gynura bicolor; HCT 116 cells; chlorogenic acid; cytotoxicity; guanosine