Magnetic resonance microscopy of chemically fixed human embryos at high spatial resolution.
We acquired magnetic resonance (MR) microscopic images of chemically fixed human embryos of Carnegie stages 16 to 22 with a large image matrix (256 × 256 × 512) using an MR microscope that we developed with a 9.4-tesla vertical wide-bore superconducting magnet and a dual-channel receiver system to extend the dynamic range of the MR signal. The images showed clear anatomical structures at spatial resolutions of (40 µm)(3) to (60 µm)(3). We concluded that the experimental technique we developed will aid construction of the next anatomical database of the collection of chemically fixed human embryos.
Institute of Applied Physics, University of Tsukuba.
Yosuke
Y
Otake
Shinya
S
Handa
Katsumi
K
Kose
Kohei
K
Shiota
Shigehito
S
Yamada
Chigako
C
Uwabe
D019412:Anatomy, Cross-Sectional / Q000379:methods; D066264:Datasets as Topic; D004622:Embryo, Mammalian / Q000033:anatomy & histology*; D005865:Gestational Age; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D059629:Signal-To-Noise Ratio